LOCUS       DACEZN010000001        61734 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEZN010000001 DACEZN010000000
VERSION     DACEZN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.001"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(71..1462)
                     /locus_tag="IXH60_000001"
     CDS             complement(71..1462)
                     /locus_tag="IXH60_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1988975.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1675..2061)
                     /locus_tag="IXH60_000002"
     CDS             complement(1675..2061)
                     /locus_tag="IXH60_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1988976.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2117..2740)
                     /locus_tag="IXH60_000003"
     CDS             complement(2117..2740)
                     /locus_tag="IXH60_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1988977.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2804..3472)
                     /locus_tag="IXH60_000004"
     CDS             complement(2804..3472)
                     /locus_tag="IXH60_000004"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1988978.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3484..3924)
                     /locus_tag="IXH60_000005"
     CDS             complement(3484..3924)
                     /locus_tag="IXH60_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1988979.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(3983..4654)
                     /locus_tag="IXH60_000006"
     CDS             complement(3983..4654)
                     /locus_tag="IXH60_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1988980.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4839..5237)
                     /locus_tag="IXH60_000007"
     CDS             complement(4839..5237)
                     /locus_tag="IXH60_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1988981.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5234..6007)
                     /locus_tag="IXH60_000008"
     CDS             complement(5234..6007)
                     /locus_tag="IXH60_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1988982.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6004..6687)
                     /locus_tag="IXH60_000009"
     CDS             complement(6004..6687)
                     /locus_tag="IXH60_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1988983.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6854..7741)
                     /locus_tag="IXH60_000010"
     CDS             complement(6854..7741)
                     /locus_tag="IXH60_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1988984.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7738..8607)
                     /locus_tag="IXH60_000011"
     CDS             complement(7738..8607)
                     /locus_tag="IXH60_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1988985.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8744..10030)
                     /locus_tag="IXH60_000012"
     CDS             complement(8744..10030)
                     /locus_tag="IXH60_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1988986.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10303..10881
                     /locus_tag="IXH60_000013"
     CDS             10303..10881
                     /locus_tag="IXH60_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1988987.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10881..11810
                     /locus_tag="IXH60_000014"
     CDS             10881..11810
                     /locus_tag="IXH60_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1988988.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11807..13543
                     /locus_tag="IXH60_000015"
     CDS             11807..13543
                     /locus_tag="IXH60_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1988989.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13606..14442)
                     /gene="pheA"
                     /locus_tag="IXH60_000016"
     CDS             complement(13606..14442)
                     /gene="pheA"
                     /locus_tag="IXH60_000016"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1988990.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14439..14948)
                     /locus_tag="IXH60_000017"
     CDS             complement(14439..14948)
                     /locus_tag="IXH60_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1988991.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(14941..16236)
                     /gene="aroA"
                     /locus_tag="IXH60_000018"
     CDS             complement(14941..16236)
                     /gene="aroA"
                     /locus_tag="IXH60_000018"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1988992.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16249..17340)
                     /locus_tag="IXH60_000019"
     CDS             complement(16249..17340)
                     /locus_tag="IXH60_000019"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1988993.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17357..18523)
                     /gene="aroC"
                     /locus_tag="IXH60_000020"
     CDS             complement(17357..18523)
                     /gene="aroC"
                     /locus_tag="IXH60_000020"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1988994.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18525..19589)
                     /gene="aroB"
                     /locus_tag="IXH60_000021"
     CDS             complement(18525..19589)
                     /gene="aroB"
                     /locus_tag="IXH60_000021"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1988995.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19622..20644)
                     /gene="aroF"
                     /locus_tag="IXH60_000022"
     CDS             complement(19622..20644)
                     /gene="aroF"
                     /locus_tag="IXH60_000022"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1988996.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21303..22277)
                     /locus_tag="IXH60_000023"
     CDS             complement(21303..22277)
                     /locus_tag="IXH60_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1988997.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22258..24141)
                     /locus_tag="IXH60_000024"
     CDS             complement(22258..24141)
                     /locus_tag="IXH60_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1988998.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24159..26384)
                     /locus_tag="IXH60_000025"
     CDS             complement(24159..26384)
                     /locus_tag="IXH60_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1988999.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26499..27533
                     /locus_tag="IXH60_000026"
     CDS             26499..27533
                     /locus_tag="IXH60_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1989000.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27600..28613)
                     /locus_tag="IXH60_000027"
     CDS             complement(27600..28613)
                     /locus_tag="IXH60_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1989001.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28691..28879)
                     /locus_tag="IXH60_000028"
     CDS             complement(28691..28879)
                     /locus_tag="IXH60_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1989002.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29319..29447)
                     /locus_tag="IXH60_000029"
     CDS             complement(29319..29447)
                     /locus_tag="IXH60_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1989003.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29450..30823)
                     /gene="rlmD"
                     /locus_tag="IXH60_000030"
     CDS             complement(29450..30823)
                     /gene="rlmD"
                     /locus_tag="IXH60_000030"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1989004.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(30934..31989)
                     /locus_tag="IXH60_000031"
     CDS             complement(30934..31989)
                     /locus_tag="IXH60_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1989005.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(31999..33429)
                     /gene="gatB"
                     /locus_tag="IXH60_000032"
     CDS             complement(31999..33429)
                     /gene="gatB"
                     /locus_tag="IXH60_000032"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1989006.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33426..34898)
                     /gene="gatA"
                     /locus_tag="IXH60_000033"
     CDS             complement(33426..34898)
                     /gene="gatA"
                     /locus_tag="IXH60_000033"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1989007.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34898..35203)
                     /gene="gatC"
                     /locus_tag="IXH60_000034"
     CDS             complement(34898..35203)
                     /gene="gatC"
                     /locus_tag="IXH60_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1989008.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35309..37345)
                     /gene="ligA"
                     /locus_tag="IXH60_000035"
     CDS             complement(35309..37345)
                     /gene="ligA"
                     /locus_tag="IXH60_000035"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1989009.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37362..39596)
                     /gene="pcrA"
                     /locus_tag="IXH60_000036"
     CDS             complement(37362..39596)
                     /gene="pcrA"
                     /locus_tag="IXH60_000036"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1989010.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39751..41310
                     /locus_tag="IXH60_000037"
     CDS             39751..41310
                     /locus_tag="IXH60_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1989011.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41421..43352)
                     /locus_tag="IXH60_000038"
     CDS             complement(41421..43352)
                     /locus_tag="IXH60_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1989012.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43370..44284)
                     /gene="pfkB"
                     /locus_tag="IXH60_000039"
     CDS             complement(43370..44284)
                     /gene="pfkB"
                     /locus_tag="IXH60_000039"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1989013.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSETETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44281..45033)
                     /locus_tag="IXH60_000040"
     CDS             complement(44281..45033)
                     /locus_tag="IXH60_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1989014.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45192..45401)
                     /locus_tag="IXH60_000041"
     CDS             complement(45192..45401)
                     /locus_tag="IXH60_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989015.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45412..46740)
                     /locus_tag="IXH60_000042"
     CDS             complement(45412..46740)
                     /locus_tag="IXH60_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989016.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47105..47692)
                     /locus_tag="IXH60_000043"
     CDS             complement(47105..47692)
                     /locus_tag="IXH60_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1989017.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47775..49025)
                     /gene="clpX"
                     /locus_tag="IXH60_000044"
     CDS             complement(47775..49025)
                     /gene="clpX"
                     /locus_tag="IXH60_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1989018.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49272..50561)
                     /locus_tag="IXH60_000045"
     CDS             complement(49272..50561)
                     /locus_tag="IXH60_000045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1989019.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50715..51647)
                     /locus_tag="IXH60_000046"
     CDS             complement(50715..51647)
                     /locus_tag="IXH60_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989020.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51804..52463)
                     /locus_tag="IXH60_000047"
     CDS             complement(51804..52463)
                     /locus_tag="IXH60_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1989021.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52466..53491)
                     /gene="sppA"
                     /locus_tag="IXH60_000048"
     CDS             complement(52466..53491)
                     /gene="sppA"
                     /locus_tag="IXH60_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1989022.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53504..53794)
                     /locus_tag="IXH60_000049"
     CDS             complement(53504..53794)
                     /locus_tag="IXH60_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989023.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54203..56008
                     /gene="glmS"
                     /locus_tag="IXH60_000050"
     CDS             54203..56008
                     /gene="glmS"
                     /locus_tag="IXH60_000050"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1989024.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56080..57435)
                     /locus_tag="IXH60_000051"
     CDS             complement(56080..57435)
                     /locus_tag="IXH60_000051"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1989025.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57459..58628)
                     /locus_tag="IXH60_000052"
     CDS             complement(57459..58628)
                     /locus_tag="IXH60_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989026.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58625..59512)
                     /locus_tag="IXH60_000053"
     CDS             complement(58625..59512)
                     /locus_tag="IXH60_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1989027.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59701..>61734)
                     /gene="nifJ"
                     /locus_tag="IXH60_000054"
     CDS             complement(59701..>61734)
                     /gene="nifJ"
                     /locus_tag="IXH60_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1989028.1"
                     /translation="LGRRINTAMETAFFKLADIIPFDEVLPLLKEEALKSYGHKSMAI
                     VEKNIQAIDRTTELLHQVAIPDHWRTLEMPEKINKRNTGYIQEILEPVNAQEGNQLSV
                     GTLVANGMTTGEMPLGTASMEKRGVALEVPEWISDRCTMCNECAFVCPHAAIRPFLAD
                     EEEMEEAPEGFIVREMRGADGVKYRIQVSVEDCTGCGLCVEACPAKEKALVMKPYEEQ
                     KEQAVNWAFAMTLRQKANPVKGKNTVLSTQFNQPLLEFSGACSGCGETPYVKLLTQMF
                     GDRMMIANATGCSSIWGGASPASPYTTNECGQGPAWSNSLLEDNAEFGYGMLLAAQTR
                     REALASSIKEVLPKVSEELRELMEDWLVHMFESEGTQQRAAKLQAAIESERSIFPELE
                     DIYKRKDLFVKNSQWMIGGDGWAYDIGFGGIDHVLASGADVNILVLDNEVYSNTGGQT
                     SKATPASAIAKFSASGKYASKKDLGMMAMTYGNVYVAQIASGANQMQTIKAFEEAEKY
                     PGPSLIIAYTPCITHGLLGGMKESLKEAKEAVSSGYWSLYRYNPTMVEKNKNPMTLDY
                     KKPDFSAMPDFMRKQVRFSSLDNANPNLAKALFEKTVHDAKTRFYNYAALTGELEKIK
                     KRLEPEESIDVPVKTRKEKTTDLEAEERRAARRAARAERRKKMEQATKEKKEK"
BASE COUNT        17094 a        13410 c        11001 g        20229 t
ORIGIN      
        1 gagaaactat ttttaggaaa cttcttctta tttcttaaag ctgaccactt ctatcacaac
       61 ctctttttga ctatgcattc acatcgatca ctttagaatc atcattgatg acatcgcgat
      121 ctaaatcttt gatgcgtgca cttgtcacga taccagctac catgctaccg ctgacgttta
      181 ctgctgtacg tgccatatcg attaatggtt caacagagat aactaaccca acgattgcta
      241 ctggcaagtt cattgcccct aaaacgatca gtgaagcaaa tgttgcgccc ccaccgactc
      301 cagctacacc aaaagaactg attgttacaa ttgccacaag catcaaaata aattccaaac
      361 taaagacatt cactccagct gctggtgcga cgatcgttgc caacatagct ggataaacgc
      421 cagcacagcc gttttgtcca atagacaaac caaaacttgc tgagaaatta gctgttgcat
      481 catctactcc taaagctttt gtctgagtct cgatattcaa tggcaaagct ccagcacttg
      541 aacgtgaagt aaacgcaaag ctcaataccg gaaaggcttt tttgaaatag ttgattggat
      601 tgacttttgc accaatcaaa atcagtgagt ggacaatcaa aacagcaagc aatgctacgt
      661 aagaagcaac gacaaattta cctaaattga cgatagctgc aaaatcactt gttgccaaaa
      721 cgtttgtcat caaagcaaat actccataag gtgtcaaacg caaaacaagc gtcacgatcc
      781 gcataatgat tttatacaga ctgtcgacta aatctgaaaa gaatgctgct tcttttggcg
      841 cttttctttt cacgcctaga taagcgattc cgacaaatgc cgcgaaaata acgactccaa
      901 tcgtacttgt tgcacgacta cctgctaaat cagcaaaaac gtttttaggg ataaaggata
      961 aaatctgctg tggaatcgat aagttttcaa ctgtttcctg acgtgttgcc agttcagaga
     1021 ttcgtgctgt ttctgcagct ccttgtgtga atttagctcc ttggagtccg aacagcattg
     1081 ttgaaccaat tccgatcaat gctgcaatag ctgttgttcc aagtaaagaa gtcaatacag
     1141 tcgcactaat tttccctagc ttttcagaaa ctttcatttt tgtgaatgca ccaatgatcg
     1201 aaataaacac taaaggcata actagcattt gcaagaatga tacatatcct tgacctacaa
     1261 tcccgatcca ttccattgat tgagtaacga ctttgtcgtc tacaccaaag atcaattgaa
     1321 gcagaccgcc aaagacgata ccaactccta atgctgtaaa gacgcgtgtt gaaaattttg
     1381 tatggcgctt ttgcatacga tagaacacat ataataatgc cgcaaaaact agaaccacta
     1441 aaactgtgat gagtgttgtc atttttctcc tcctcatatt attaatgaca agtccatcga
     1501 aggaagtaaa ggttcccgag aatgattctt caaccatcct cctgaaaaaa tttttttcag
     1561 tgacttttat attttagacg atttttatcg aaatgacatt ctttttgctt acaattagta
     1621 ataaaaaaac atacctgtga tagctttaat ctatcgcagg tatgttttta ctgtctattc
     1681 ttgtttttct gattgaaaat tcaattttac cccaggctga tctaaggcga tctctgtcac
     1741 ctcataagac attcggcgta cgatatcgaa taacggtgct actagctcgt ccacttcttc
     1801 ttgtgtgaca tcattttgac cttcaatttt ccgattgagg atatgattga tctgactcac
     1861 acttccagat aaaacatatg tatcaaacac taaacggaac tctaagcgtg ctcctacgat
     1921 gctatgttct ttcggatagt tttcgatcgt ttcttttaaa ggcgagaagc ctactttcaa
     1981 atctgtttct acttcttggt tcggcaatct catatcataa tgaaatgctt ctacaacttc
     2041 tttttgtctt ctgatttcca tataatttta tcactccttt atgaaaatta taacatatct
     2101 cctttgaaag gggatactac tttttaatga tgctctgcaa ggtaaggatt tcattcagat
     2161 ttcctaccac ataatctgcc gggatctcac ccagaaaatg atcgatgtcg taaagacaag
     2221 tagcgactcc tgcatttttc cctgcttcga tatctaaacg acgatcgccg atcatcatcg
     2281 tgtctgttct ttctaaatga aaagtatcga tcaaataatt taaagatgct ggatcaggtt
     2341 tacgaggaaa gtcctgatcg atccctacta cttcctcaat cagatgtgcc aatcgatgtt
     2401 cttttaacag gccccatgtt gattctgtca aacggtgggt caggatgaaa tgacgaccgc
     2461 cgttgtcttt tagcatttcc agtacttgtt tcgtttctgg aaatggacgg gaaacttccg
     2521 tactttcttt ttcatatcgg tgaaataatt cattaaactc tctggcattc aagtttgatt
     2581 cattgatcaa ataactagta gaatactttt tcattttgaa gtagatttct tttttgtcca
     2641 tactgatacc aaaatctttt agtgcttgcc atgcaccatc taccattgcg ggatacgtat
     2701 caaacaatgt accatcaaag tcccatatat aatttttcat gttccttttg ttccattcct
     2761 ttgcttcttt tttctatctc tcacttttag ataaaggtgt tctttaaagt atttttttgt
     2821 ccagaattgt caaatcatct gctaatgtat actggattgg ttgtgcattt gggatatcga
     2881 tcgtatccat ttgatcttca ggaacatctt ctaaatattt tgtcagtgct cggatgctat
     2941 taccatgacc cacaactagt aaatcttgcc gatcttttag ttttggtgct agatgatctt
     3001 gccataaggg aatgatccga ttcactgtca tttccagact ttctccgtat gggatcaaat
     3061 gcggatccaa gtgatcatat ctgcggtcaa atctattttc tgcggttaac ggcggaactt
     3121 ccgtatatcc tctgcgccat gtttttactt gatcttttcc gtatatttct ttcatcttat
     3181 ctttgttttt acccacaaga tccccataat gccgttcatt tagacgccat gtttttatta
     3241 taggaagcca taactgaccc atttcttcta gtgcgacttg tccggtaaga atcgcacgtt
     3301 tcaaaacaga ggtataaacg gcatcgaatt gaagtccggc ttcaagcatt ttttgaccgg
     3361 caactgctgc ttgttctttt cctttttctg ttaaggcgac atctagccat cctgtccagt
     3421 aattttcaaa atttgcttcg ctttcgccat gacgtaataa tactaatttc attcttttct
     3481 cccttaaaca ttcgtttcga attttatacc acatgttttg catgtcacac gtattcgtcc
     3541 ttttccttta ggtgcacgct gcttttgatg acaattcggg cactcaaaaa aagtataact
     3601 ttttcgatcc attttgactt gctttttgtt ttttatctga tgatattttt ctttgactct
     3661 atcaagatat ttcaaataac gttgattctc atttagtctg gggtagattt tttttgatag
     3721 gaaacgaaat atcatccatc cgaaaaagac gatgaacaac aattgtccga ttctataggg
     3781 caaccaacga cttagcaaac taaagacaag cgcagcgatc aatagaccgc gattcaactg
     3841 atcaaatttc gcatatcttc cctgcataag tttgtgatag ttctgctgaa atcgtattag
     3901 cctttctaac catgtttgca tcatattatc tcctccatca tctaatattt attatgacaa
     3961 aaaaatccaa aaagaaaaag ccttaattgg ccttttccat tgcaacttgg tgaaggacat
     4021 tccgatttct ggtaaagtca atcacaatca ctttcttttt atcttctctg ttgactactc
     4081 gaaatgtgtc cgtcaatgga agaaacagct tcttagatgg tagctgttgc atacgtataa
     4141 aacgatggat catcgtaatc aattgaggaa attgaataga cgtgtgaagc agacctggat
     4201 attttttcaa cagctgtgtc gttttgatca gattccgttt tttactgatc acttttaaaa
     4261 agctttttag aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt
     4321 gataacgcat ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat
     4381 cgatcattaa accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa
     4441 gctcgatata atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg
     4501 acagcccttc tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg
     4561 aaaaatgcag caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa
     4621 tcagagtatc ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt
     4681 taaaatagca tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta
     4741 ctatttcata agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg
     4801 ccttcttgtt tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta
     4861 tcgttatttt catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta
     4921 gtgataccaa tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg
     4981 atgttgatgc cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat
     5041 gtcaacacaa tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt
     5101 acaccagaac ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag
     5161 tcgataacat cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat
     5221 gctacaagat ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt
     5281 ctttttcatc ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca
     5341 atgcaatcat tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg
     5401 cagcgattgc cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc
     5461 caccgttatc aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg
     5521 ctagcccgta tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca
     5581 tagacaatcc tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc
     5641 ggattctccc ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag
     5701 caaaccctac taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca
     5761 atcccgcaat agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt
     5821 gcaggaaaag gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac
     5881 gctttttgcg catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg
     5941 ccactgccaa aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga
     6001 aactcattgg aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat
     6061 accgaccatt ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc
     6121 tccggcaagg gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc
     6181 aaaagcaatc ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta
     6241 gcctatagcc atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc
     6301 tagaaatact ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc
     6361 gtcagatata tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat
     6421 cacgatcccg atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat
     6481 atatttccct actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag
     6541 cgtcattccg atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc
     6601 taacggtgca aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc
     6661 aatcacttct ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt
     6721 aaaatgaaag agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata
     6781 aaataacaca cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc
     6841 ttattgtgtt tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa
     6901 caaaaaatac gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat
     6961 agtctggtgt ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag
     7021 gagattgcaa agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga
     7081 ttgcagccgc ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta
     7141 gttcttttag accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat
     7201 tttgtcgaaa gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga
     7261 aactgttgat tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca
     7321 tcaatgccgc aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc
     7381 gtttactttg ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca
     7441 cagtggtcac caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat
     7501 tggaaaacaa attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg
     7561 tgatatccac gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa
     7621 taaatgatag gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt
     7681 ctaatgcttg ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca
     7741 tcgactttct cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat
     7801 aacaaaggaa actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata
     7861 gatgtattga gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac
     7921 ttcatcaaaa agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg
     7981 ttttaaattt gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga
     8041 tgctgcttca taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt
     8101 gtatccagtc caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg
     8161 agtcaaccag ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag
     8221 gccagattga gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca
     8281 tggaagaaag atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag
     8341 gttggaaata aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa
     8401 tgtattaaaa aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa
     8461 cccgttaaat gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac
     8521 catcggaata aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc
     8581 gtttttgaat aaatctttct gcttcattct tttccctcca attctatcat tgattatcta
     8641 catgaaaaga caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt
     8701 ttgtctgatg aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg
     8761 cttctacttg tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc
     8821 gatgaagagc ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt
     8881 ctggtatttc agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt
     8941 cagaagcttc ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga
     9001 tttctttcgc aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat
     9061 ctttcgctga atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt
     9121 tagaagcttg cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt
     9181 ttccagattg gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag
     9241 aagctactgc tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga
     9301 ctagatcttc tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt
     9361 cttggatcgt gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa
     9421 tcatccttac tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt
     9481 ctttgacatc tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt
     9541 ctgtataacc tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat
     9601 cgaaaggaac accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa
     9661 aatgatcttc ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat
     9721 agccttgtat cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc
     9781 tcgctaaagc ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta
     9841 tctctgtttc ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg
     9901 tctcatccca cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc
     9961 cgcatcctgt taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc
    10021 tgatgttcat ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca
    10081 tcataccaaa atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa
    10141 aataataaaa aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa
    10201 gatcgaaatg gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa
    10261 gtatttttaa attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt
    10321 cttatcgatg atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag
    10381 cacgggtttc aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg
    10441 gtacatgcaa tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa
    10501 ttatcgcggc tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat
    10561 gatgaatttg atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag
    10621 cctcgattga gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa
    10681 aaaaagccca acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca
    10741 cagcagattg ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg
    10801 tttttctgtt atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac
    10861 agtcgatcac ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta
    10921 ccttttcgga agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt
    10981 ttgttcaaaa gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt
    11041 ttgcaatcca agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac
    11101 gtttctatca taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca
    11161 accacgagca gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag
    11221 ctgtcaaaga acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt
    11281 atttgaaaca tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc
    11341 atgttgggaa tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg
    11401 ctgagtattt cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact
    11461 tttctgcaaa tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc
    11521 aggaaaatta tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat
    11581 atagctatct ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa
    11641 aaaaagtccg tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg
    11701 aaatctgtga gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag
    11761 agacaggaat gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat
    11821 caaagagcat gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca
    11881 agccatcgtc atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt
    11941 caatcaatcg aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga
    12001 tacaatcgag aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc
    12061 caatgatcca gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa
    12121 tgactctctt tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa
    12181 tggaagaact tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca
    12241 atctgcgatt gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca
    12301 ccacggattg actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa
    12361 aaatacattg aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc
    12421 gaatcttttt caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga
    12481 acagacgatc atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa
    12541 aaaactaaca gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct
    12601 gtaccgccag gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat
    12661 gggcgtgatt tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt
    12721 ggtatttatg tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa
    12781 acggctagag gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca
    12841 attgctaggg aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac
    12901 gatccaagac gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca
    12961 gccccatttt atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac
    13021 agatgaagca gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa
    13081 aaaagaagaa gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct
    13141 tttgagtatg cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca
    13201 gtcattatct atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc
    13261 ttttcacaat aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct
    13321 tttattcgaa gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa
    13381 tgatcatttt tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg
    13441 cgatgcatat ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg
    13501 tctaccgctt cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg
    13561 atttttttat tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc
    13621 ttctactgtc agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat
    13681 ttcttggagc gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa
    13741 aaaatattct cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc
    13801 ccgccagcca aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt
    13861 caagatgaca ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac
    13921 gatccaaaaa cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa
    13981 tccaacatgc tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc
    14041 tgctacatac attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata
    14101 atgcgtttct aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt
    14161 aatctttgtt gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt
    14221 aacttcgact tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa
    14281 tgaattctct acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca
    14341 aacagggatg gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg
    14401 ataagtgaat gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct
    14461 ataatggtat cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca
    14521 ctttcttcat accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt
    14581 ccaagagcta aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc
    14641 gcttctgcct ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca
    14701 ctttggacaa tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa
    14761 atatctgact ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc
    14821 gacaattgga tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat
    14881 tgagctaacc ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg
    14941 ctagccatga gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata
    15001 agagattgaa acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc
    15061 tacttgcagc atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag
    15121 aggtgtcggc ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt
    15181 ctgtaattct tctgccaccg catcaattct gttggtttct tttactttta attcttctgc
    15241 gtctttgatg atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc
    15301 atcaatcaga cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt
    15361 gacacttaaa tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc
    15421 catctcctcc aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa
    15481 caacagttgg ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat
    15541 atcacctgga acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt
    15601 tttatcttcc actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg
    15661 ggaacgttct ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa
    15721 agcagatttg acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg
    15781 ggttcctgtc acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc
    15841 catctcgcgt aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc
    15901 gaacagagta gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt
    15961 accggagttt cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc
    16021 atgaatcttg atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg
    16081 taaagtactc agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc
    16141 aattgctcca aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt
    16201 ccctcttaat ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca
    16261 gcaatgataa ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt
    16321 tttaaaactg atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt
    16381 aatcaaagaa actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc
    16441 atctggcaca tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg
    16501 caactggtct cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc
    16561 agtcatgatc cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa
    16621 taatatctgc ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat
    16681 cctagtgata tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt
    16741 gaattgttct gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat
    16801 tcctgttata cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca
    16861 aaatagttct tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta
    16921 ataggcattt tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc
    16981 cattggatgt ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg
    17041 cttcgtactg ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac
    17101 atcaagataa gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc
    17161 tgcacctgtt tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc
    17221 acgggttttt gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat
    17281 acaaagtgcc atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat
    17341 cttatttgcc ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt
    17401 tcaactcaac aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg
    17461 ctacaacatt ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta
    17521 cacttgcttt gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg
    17581 taggaatggg tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt
    17641 cgaagccgcc tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct
    17701 catccatcac ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc
    17761 ctttaaaggc attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc
    17821 attgtacgta gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc
    17881 cgatcgtatc tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg
    17941 ctggatcaac aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga
    18001 tatttccagg tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga
    18061 tgtcaagttc ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct
    18121 ctcttgcaga ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc
    18181 ctaccaaatc cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt
    18241 caggcacttc tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca
    18301 ctaaagtgac aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac
    18361 gatctttttc gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt
    18421 cgtgattgat ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg
    18481 ttaattctgg tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag
    18541 taatctttga atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc
    18601 aagatgatat tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat
    18661 tcatctggat gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct
    18721 aaagcagctg tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct
    18781 accattccaa tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca
    18841 attgcatggc caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca
    18901 tcttcttcca ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt
    18961 tccagaagat ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct
    19021 atagctgctg atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt
    19081 tttagaacct caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt
    19141 ttcgctttgt ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt
    19201 agcgtagtcg gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct
    19261 gctagatcac ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca
    19321 ttatctgcta aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc
    19381 gccggtacga ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt
    19441 tctgcatata gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa
    19501 atcgtttgaa gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat
    19561 ttttggttat caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga
    19621 attaatcgag tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca
    19681 tgcgtgagta agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat
    19741 caggatggat ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg
    19801 gtggtacaag ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag
    19861 ttaatttttt catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt
    19921 aagtccgaat cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt
    19981 ctgcagcatt aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt
    20041 tacctgtttt accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct
    20101 gtaggatatc gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa
    20161 tcactttcat tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta
    20221 aaccttggaa agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg
    20281 cgcccccggc tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac
    20341 aaggaccagc catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga
    20401 ctgtattgcc aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag
    20461 cattttctac tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt
    20521 ttagtccaat cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt
    20581 cgatatgatc gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta
    20641 tcattgtttt tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt
    20701 ttcaaggtgg ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg
    20761 ctccttcttt cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat
    20821 tgcctctaca gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc
    20881 cactatagat gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat
    20941 agatacaaac ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag
    21001 ctaaagtaat acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa
    21061 ctgatgtaag aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg
    21121 aaactttttt taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt
    21181 attgaagaaa atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga
    21241 catatagaat aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat
    21301 tgctactgaa taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac
    21361 tcaacatccg ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta
    21421 agaaccgttc gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct
    21481 gctttgaatg gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa
    21541 aaatcttctt gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat
    21601 ttcgtttcca tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct
    21661 gccactttat aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat
    21721 aggccttcat actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg
    21781 ggtaattccg gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct
    21841 ccagcgattt ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta
    21901 aaaatgattt caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca
    21961 atcggcaaat cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac
    22021 aatccggctc ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg
    22081 gtcgttgctc gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta
    22141 ttgagcgatt ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg
    22201 acacctaatg tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta
    22261 tttactgtat ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc
    22321 tgcaaaaacg atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc
    22381 gccatagcgc atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt
    22441 aaaatcccat atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga
    22501 atccattcca cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt
    22561 ttccgcttta ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt
    22621 tacatctgaa atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag
    22681 tcgatcgggg tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata
    22741 agattcattt ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt
    22801 cgtttttgct cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta
    22861 agaaggctca aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc
    22921 catcacatgg attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg
    22981 agatgtccgt acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc
    23041 ttctatagtg atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat
    23101 aatcatccgt tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc
    23161 gaaggaatag tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag
    23221 cttatataaa ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg
    23281 aatcgtcatt tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc
    23341 tagtatcgct acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc
    23401 aagtttgcca ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg
    23461 gttttgtcct cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc
    23521 ctgatcttcc aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc
    23581 ttcttccaaa taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc
    23641 gataaaatga ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga
    23701 gatttgtttt ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg
    23761 attgtcttta gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg
    23821 gggacggcga aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc
    23881 ataatttgct aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa
    23941 atctttttta taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca
    24001 tgatccattt aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata
    24061 acaaacatga tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa
    24121 ccattttata tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc
    24181 agttctgttt ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg
    24241 gcccccgaaa atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc
    24301 atcagatata cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca
    24361 atagcttcat cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga
    24421 ctcgccaaac gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata
    24481 atttggtctt ttacttgctt ttcttcttca ggagacagct ttgtcaaatc cagttcataa
    24541 ccaaaaacag cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc
    24601 tgatgatttg gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt
    24661 gttccatatt ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc
    24721 ggcatataat aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat
    24781 aagatttccg gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa
    24841 cgatgcgcta cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc
    24901 gaacggttca tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt
    24961 tgtttctcaa tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg
    25021 gaagcagaag gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt
    25081 tcagaatcaa ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta
    25141 tgagcataat ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag
    25201 tctcctaatg aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac
    25261 atttcgatgc ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag
    25321 tcaaagtaag tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga
    25381 cctcttgcga ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc
    25441 tgacttgagt aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc
    25501 cacgaaaaac gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt
    25561 tcaagttcga aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta
    25621 ttttctgtcg tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt
    25681 cctcgacggc tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt
    25741 tcattgacat gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag
    25801 tctagctgga aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc
    25861 tttacagatc gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa
    25921 atcgctgttt ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc
    25981 acataagttt gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca
    26041 tatctcagat ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga
    26101 aagtccatgc tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg
    26161 tcaggattta agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg
    26221 tctttgtact gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct
    26281 atctttatca gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca
    26341 aataagatga aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca
    26401 aaacggaaac cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt
    26461 caataaaaat tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg
    26521 cacgaaaggc aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga
    26581 tccatgtggc tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg
    26641 acatcagtac tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga
    26701 tcggcaaagc gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag
    26761 aagaagctcg acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta
    26821 aacaatggga agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag
    26881 aatcgattac cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca
    26941 tccagcaaga ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg
    27001 aattgttctt agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata
    27061 tggatgaatt cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc
    27121 attttatgat cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag
    27181 aaaaacaagg tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg
    27241 ccattcttgt cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa
    27301 atatccaaat tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa
    27361 acctctcccc tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg
    27421 ttcgaatggc attggagcga attgacggta tacgggacga accaatcatt ttgacttatc
    27481 ctgcacgatt gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag
    27541 gttgtgaaaa gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct
    27601 tacttatact tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc
    27661 gtgtaatcaa attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat
    27721 tgcacgggta cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat
    27781 gagtatgcat ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc
    27841 acactagctt ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac
    27901 gcaacggttt tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc
    27961 ttcaaatcaa caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtaga
    28021 tgtttgattt tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat
    28081 gcgatttgtg aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg
    28141 tcgtcttttt cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta
    28201 gggtattctg aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct
    28261 gaaactaatt ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca
    28321 ccatgtaccc aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca
    28381 taagtattct ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt
    28441 tctgcttgat cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg
    28501 atcaagaatg ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc
    28561 ccaagaatga ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc
    28621 cctcagtagt tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct
    28681 cctagatgat ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt
    28741 ttttccattg ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag
    28801 gacttaagca caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca
    28861 cttgctggtg ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat
    28921 ttatcttcag agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct
    28981 aaataatagg cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc
    29041 cctgatgccg ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa
    29101 tcatttgaga atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa
    29161 cgaaaaatct gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa
    29221 cagtccttat tggcaaatcg ccaaaataat gtagactaac taaaaagact aagaaacaat
    29281 aacaaaagtt aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg
    29341 ccttttctca tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca
    29401 ccttgtttct ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt
    29461 taacgaatga agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa
    29521 ctttcttcac ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg
    29581 gattacatga gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat
    29641 gatgatctaa tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt
    29701 cttgccattt tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt
    29761 tattatttaa agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat
    29821 acaccttatc cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt
    29881 cgatcacaat gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact
    29941 ctgcttgttg agtatttact tgataaaatg actttgctga gattcgatag gtttttccta
    30001 atagctgatc ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca
    30061 catttgtttt ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat
    30121 gaatagcttc agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga
    30181 ccatcatttc atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat
    30241 ttttttcttc attatatcct cgtacttgga accgttgcaa gatatctctg actactttga
    30301 ttgcttgatc gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc
    30361 tattcttttt gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg
    30421 ctttattacg gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg
    30481 gcatcttagc aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt
    30541 gttcatactt caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta
    30601 caggctgacg atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat
    30661 tgcctgcttt tactactagt atttctactt cttctccagg gagtgcattt tcgataaata
    30721 atggataacc atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga
    30781 ctgtatatcg ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc
    30841 ctatttttca atcctactga actctactat aaatcagcta attacacaac taaaaccggc
    30901 actgacctta ctacgttcag caccggcttt tgattaattc ttttgattag gtgtttcgtc
    30961 tgctattttt ccgtcaccgt cgatatcttc atccgttaaa cgttcgactt cttttacgaa
    31021 ttctttgctg acaacctcta attcttcttc atctgtacct gtgattgctt catcaggaat
    31081 ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga ttttggaaat gcatcggtgc
    31141 ttccccgccg tattcaccat ctaagttgat cattactggt cgattgatat catcttctgt
    31201 ttcaacgaac aaactgcttg ttttggtata gattattctt ggatcatcga catgtcgacc
    31261 gccattcagc atgagtgctg caatatgcaa gatctcaaag atatttgctg tcttcacaat
    31321 gattaaagaa aacttcccat catccaattt ggcatctggc gcgatctgtt cgaatcctcc
    31381 aacagagttc gtcagcccta ggaaaaacat cgaagcattt ccgatatatt ccccttcatc
    31441 atacttcaag cgcattttga ttggctttac tcgagggagc atctctgctc ctttcgcaag
    31501 ataagcaaga taaccaaaaa tactctttag ttctgatgga acttcataag tcaattctgt
    31561 caaatagcca cctgcagcga tattgatgaa gtaactgtta tcagaaaggc cgatgtccat
    31621 ttttacggtt tggtgttttt ttatcacttc tgctgctgct ttaatattat ctctaggaat
    31681 ttttaatgct cgagcatagt cgttcgttgt accagctggg atgatggcca ttttaggacg
    31741 gtgttcaagc ggagcgattc cattaaccac ttcgttgatt gtgccatcgc caccagctgc
    31801 cactactaga tcaaaaccta gttctgcgac tctacatgct tcattcttgg ctgaatctgg
    31861 ttctggtgta gttgcatagg cgctggcttc aaatcctgct tcttctaatg cctgtaggat
    31921 atccgccaaa ttttttttca ataattcttt tcccgaggtg gggttataaa ttacgcgtgc
    31981 ttttttcatt ttcagcctct atcgtttcgc taattcatct ttaagtagct tattcacgac
    32041 accaggattt gcttgtcctt tagttgcttt catgatttga ccgactagga agccaaccgc
    32101 acgatctttt ccatttttga aatcatcgac agattgttgg ttgttatcca aaatttcatt
    32161 aatgattggc agtagtttgt tcggatcaga taattgcacc caaccatttt tttcaactac
    32221 ttcttgggct tctccgccat ttgtgatcaa ttcacggaag acttttttgg cgatttttga
    32281 gctgatcgtt ccatcggcga tcaaacgaat cattcctgct aagttctcag gtgtcaattt
    32341 tgtctcagct aattccagtt tctcactgtt caagtaagca gaaacttcac ccatcaacca
    32401 gttagatgct tgcttggcat ctgcaccatt ttccaaaaca gcttcaaaaa aatcagacat
    32461 ttcttttgta agtgtcaata ccattgcgtc atactctgga agtccaaact cacggatata
    32521 gcgttcgcga cgagaagctg gcatctcagg caaactagct tttacttttt gaatccattc
    32581 gtcatcgatc acgaatttcg ggatatcagg ctctgggaag taacgataat cactagaccc
    32641 ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg tcaaaacggc gtgtttcttg
    32701 ttggatttct cctcctgaaa gaagaacttt tgcttgacgt ttttcttcaa aagctaaacc
    32761 ttttttcacg aaagataaag agttcaagtt tttcaattct gctttcgtac cgaactcttc
    32821 ttgtccataa gggcgaagag aaatattggc atcacagcgc atagagcctt cttccatttt
    32881 cacatcgctg acttctgtaa attggataat tgatcgcaat gcttctagat aagcgtatgc
    32941 ttcttctggt gaacgcatat cagcttcaga aacgatttcg atcaaaggcg tgccttgtcg
    33001 gttcaaatca acgtaagaat aaccgccaat accatgcatg ttttttccag catcttcttc
    33061 taaatggaca cgttcgatcc ggattttttt ctttttacct tctacttcga tttcgatcca
    33121 tccgtcatga ccgattggtt gatcaaattg ggaaatttga tatgcttttg gattatctgg
    33181 gtaaaagtaa tttttccggt caaaatgagt atctttagag atttcacagt ttaatgccaa
    33241 agctgctttc atcccgaatt ccagcgcagc tttattcatt actggcagca cgcctggata
    33301 accccagtcg atcacgtttg tattgctgtt tggttctgca ccaaaatgag ctggcgctgg
    33361 agagaaaatt tttgagttgg tttttaactc gacgtggact tctagtccaa tgactgtttc
    33421 aaaattcatt cgtctttccc tcctaaaata gctggttgtt gtttatggaa ttctgttgct
    33481 tgttcaaacg catatgccgc ttgatacatc gttttttcat caaaataatt accgatgatc
    33541 tgcaatccaa ctggtaatcc ttctgataaa cctgccggta cagacatacc tggaagtcca
    33601 gccaagttga cagggatcgt taagatgtca ctcatgtaca ttgtgattgg atcgttaatg
    33661 ttctcaccga ttccgaatgc aactgttggt gaagatggcc cgatgatcaa gtcataatct
    33721 gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga cttgtcctgc ttttttgaag
    33781 taagcgtcat aataacccgc gcttaaggag aatgtaccta acatgatacg gcgttttact
    33841 tcttcaccga atccttctga acgtgagttg acatatacat catctaatga tttcacattt
    33901 tcagagcgat aaccgtagcg gataccatcg aatcgttgca aattggaact tgcttctgat
    33961 gaggcaatga tataataaac agccacaccg tattttgaat gaggcaagct gacttcttcc
    34021 acaacagcac ctaatgaacg gaatgtttct gctgctttca atacagcttc ttttacgcca
    34081 ggggccacac cttcgcctaa gtattcttta ggtaaagcaa tcttcattcc tttgatgtcg
    34141 cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg aagaagtacc gtctttttcg
    34201 tcataaccgc tgatagctgt caatgctaat gcattatctt tcaccgtacg ggtcattggt
    34261 ccgatttggt caagagatga agcaaaggca atcaaaccaa aacgtgagac acggccataa
    34321 gtaggtttca tccctacgat gccgttaaaa gctgctggtt ggcggatact tcccccagta
    34381 tcactaccta aagaaactgg aatttgtcca gctgcaactg ctgctgcaga accaccagaa
    34441 gagccaccag gtacttttgt tgggtcccaa gcattttttg ttttcttgaa ataagaagtt
    34501 tctgtacttc cacccatagc aaattcatcc atgtttagct taccaacagg gatcatatct
    34561 gcttgataga ttttatccat gaccgttgca tcataaattg gttcaaaatt gtaaaggatc
    34621 ttagatgcag cggttgtcaa gatatctttt gttacgatat tatccttgat accaattgga
    34681 ataccagcaa ggacgttgct ttcatcaatc cctttttcat ctaaagcttt tgcctgttcc
    34741 aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct caggctctgt ttctttgatt
    34801 cgttcaaatg ttgtttttgt caaatcttgt acagtgattt ctttcgatac aagcatatga
    34861 tgcagttctt ctagagaacg attgtgtaat tctgtcatta tgcgccagcc tccccattat
    34921 cgatgattgc gggtactttg ataaagccgt cttctgtttc cggcacattt ttcaataatt
    34981 catcgcggtt ccatccagct tgcgcccgat cttgtcgcat cacgtttact gtttcaacca
    35041 cattcgacgt aaatggaaca ccttccgtat ctacttcttc cagcatttca accatatcga
    35101 tgattttacc taattggtcg gtaaaacttg caagttcttc atctgcgaac gcaagttttg
    35161 ctaatttggc aacgtgtttc acttgttctt cactaatcgc catactaccc cttctttcta
    35221 aacgagttaa aaactttgct ctctatttta acacaagagt gccgtgtctg aaattgttta
    35281 ttgaaatttg tctgaaaaaa ctcatttttc attcttctac tgagcgactt ttagccaatg
    35341 catctgccat ttgatcttct gtccaaactt cgattcctaa ttcttgtgct ttggtcagct
    35401 tgctgccagc atcttctcct gctaccacga tatccgtttt tttcgatacg ctaccagtca
    35461 ctttcccgcc aagattttcg atcgtctctt ttgcttcttc acgagtaaat cgtgttagtt
    35521 taccagtaag gactactgtt ttttctttaa aaggagactc gacttcttgc aattgggcat
    35581 ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt taattcattc atcaattcat
    35641 ggacttcttc gttactgaaa taagtgacta cactgtcggc aatgatatca ccgatcgtat
    35701 caagcgcaat aatcgactca tagtcacttt tactcaatgt ttctagatca ccgaaatgtt
    35761 ctgctaaaat ttttgctgct ttcgctccga catgtcggat tcctaacccg aaaatcaacc
    35821 gctcgaccga attatctttg ctgttgtcga ttgctgtcaa aatattattt gctgattttt
    35881 cttttatttt gtccaacgtc aataactctt cttctgtcaa tttgtaaaga tcagcaacat
    35941 ctgctactag ttttttatca tacatctgtt ctagtacgcg cggccccaac ccatcgatat
    36001 tcatcgcatt tcttgaaaca aagtggttca aaccttcttt catttgagca gggcatttag
    36061 gattgatgca ccgtaaggct acttcttcat ccaaatggac aagttcactt ccacaaactg
    36121 gacaatgtgt aggcaattgg tattcttcgc tgtctttagg tcgtttatct aacacgactt
    36181 gcgaaacttc tgggatgatg tctcccgctt tataaatcag aactgtatct ttcaagcgga
    36241 tatccttcaa cttaatataa tcaccgttat gcaggctggc acggcttact gtcgttcctg
    36301 ctactcggac tggtgtcatg atagctgttg gcgttactac ccctgttcga ccgactgtcc
    36361 attcgatgtt ttcgattagc gtttcaactt cttctggcgg aaacttgtaa gctgctgccc
    36421 atctcggtgc tttgatagta aaaccaagtt gatcttgtag cgagaattca ttcactttga
    36481 tgacgattcc atcgatttca tagggcaaat ccacacgttt gtcatgatat tcttcaatgt
    36541 atgaccacac ttcatcaatc gaatgacata gacgtttttc ccgattcgtg tggaatccaa
    36601 ttttttctag ctcttctagc gcatcatact gggtttttgc ttgcatagga ccgaaatcag
    36661 ctactgtgta taaaaaagtg tccagatttc ttttcgctgt gattttagaa tctaactgac
    36721 gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat atcttttccc tctgcttctc
    36781 tgtcttggtt caattggaca aatgaccgtt ttggcatgaa acattcgcct cttacttcga
    36841 tgttcattgg ttcttttaat ttgattggga ccgaccggac agtttttagg ttttcagtga
    36901 tgttttctcc tacagttcca tccccgcgag tcgcacctct cacgaaattt ccatcttcat
    36961 aacgcaaaga aacggaaagt ccatctattt ttagttcaca gcaatagtca actacacgtc
    37021 ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc ttctttgctg aaaacgtcgt
    37081 tcaaactgta aagcggaata tcatgagtga ctttttcaaa accttctaat acttttccgc
    37141 ctactcgttg agtgggagaa tcagacgtga tcaaatcagg atattgttcc tcgattgcta
    37201 ctaattctgc gtattcttta tcataaacat agtcttccac tgttggttta tcttctacgt
    37261 agtattcgcg agaccattgg ttcagtcttg tacgaagttc ttctgctcgt ttagcagctt
    37321 cgtcaatcgt catttcatgt tccatcattt accctccact actaagcttt ttcaattggc
    37381 gcaaaggcag ccaaaagacg ttttactcct tgacttggga aagccacatc tagttctaga
    37441 tccttcgacg tcccgcttac tcggacgact gttcctagac cccatttttt gtgtttgact
    37501 ttatctccag gtttccagct gttattttcc cctccagtag cacttttaga gggaacaacc
    37561 gctgtctctg gctgtttata gctatatgct gtttttttag cgaatacagg cgttttttta
    37621 ggcgtaggac gcatgccttc gatttccagt aactcctgat cgatttcttc tacaaaacga
    37681 gaaggtcggt tatattgcgt ccgtccatat aatgttctgg agaaagcatt tgttaaatac
    37741 aacgcttctt ctgctcgcgt gatccccaca tatgcgagac ggcgttcttc ctccagttcg
    37801 ctttcttcca tcaatgctcg agataaagga aaaaccccct cttctagacc aatcaggaat
    37861 acaactggaa attctaatcc tttggctgca tgcaaagtca tcaatgtcac ttgggaagca
    37921 tcttcttcta aattgtcaat gtcggaaaca agtgccagat catttaagaa gaccgttagt
    37981 ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt cgaattgttt gtcgaattct
    38041 tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg cttgtgcttc taatgtattt
    38101 tggattttta agtcttctag atatccgcta cgatccaaaa cctctttcgt caattctgtc
    38161 actgtcaagt atgggatcat ctgagtgact tcttggatca tctctccaaa tgcacctaat
    38221 tgctgtcctg cttttccacc gatatttgcc agatccacat tttgcgctgc ttcaagcagt
    38281 ggccattcat gaagagaagc aaaactgcgt agtttttcga tagatcctgg tccgatgcct
    38341 cgtttgggcg aattaacgat acgctcaaaa ctgattgaat cttgaggatt taccagcaca
    38401 ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt agaacttgtg ccctccaacc
    38461 attttataag gaatatttgc tttcaacaga gtttcttcca ttacacgaga ttgtgcgttc
    38521 gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat tgcttcgaat ctcttcttgc
    38581 atccggtcta cgataaaacg ggtttcgtct cgttcattat ctgcacggta gtaagtgatc
    38641 ttgtttcctt ctttgttttc tgtccacaag ttttttggtt ttcgattgct attgttttca
    38701 atgacttggt ttgctgcgct aaggatgttt ttcgtggagc gatagttttg ttctagcaaa
    38761 attactgcag catctggata atctttttca aaatccaaga tgttctgcat atctgcaccg
    38821 cgccagccgt aaatactttg atctgcatct cccacgacgc acaggttcct aaatctacct
    38881 gccaataaat tgactagcgt atactgtgcg tgatttgtat cttggtactc atctacatga
    38941 atataatgga atttgttttg ataatacgtg agtgaatcag gatgttcttc aaataatcga
    39001 atcgtattca tgatcaggtc gtcaaagtcc atacattgat tatattgaag ctctttttgg
    39061 taagcagcat aacattttgc cgcaatctct tcgtaataac tgccttgcat ttttgcaaaa
    39121 tcctgagggg tctgcaggct gttttttgct tgagagattg ttcctaatat acttcgagga
    39181 tcatattttt tcggatcaat gttcagttct tttagtatcc gtttcatcaa ggtcagctgt
    39241 tcagaagaat cgatgattgt aaagttccgg ttatacccga taaaatcaac atctcttctt
    39301 aaaatacgca cacacatcga gtgaaaagtc gatacccaga catcttcgcc tccagatgcg
    39361 agaatcgcat tgacacgttc tttcatttct tttgcagcct tattggtaaa agtaatcgct
    39421 aggatgttcc aaggattgac ttctttttcc tcaatcaaat aggcaatgcg gtgtgtcagc
    39481 actctcgtct ttccacttcc tgcacctgcc attagcagta atggtccgtc agtgtgaaga
    39541 acagcttctt tctgacgggg gttcatcccg tttaataatt cagctttgga tgacattaac
    39601 aacatccttt cttttatcaa tcttgttaat tatagcattt tcttggcttt cattaaacta
    39661 gctagttcaa aatcatttgt ttctttttat ggatttttcc ttgtcaattc gttgatttcg
    39721 cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa aaggaaaaat caagaaatta
    39781 gataagactc agatatattt tattctaaaa ggaatatttg ttggactgct gacaggaatc
    39841 gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg atttagttcg aagtatctat
    39901 gaaatgagtc gggaacagcc gatttactta attggcctag ctatttgctg catccttgca
    39961 gcatttttcg tgggttatct ggtcaaaaat gaaccggata tcaagggaag cgggattcca
    40021 caagtagtgg gacagcttcg cggggaatta tcaatgaact ggttctctgt tttatggaaa
    40081 aagttcattg gcggtgtact ttcagttggt gcgggccttt ttctaggtag agaaggacct
    40141 tctatacagc ttggcgcatc cattggacaa ggagctggac aattgtttcg gagtccatct
    40201 tcagaagaaa agattttaat ttctagcggt gcaagtagcg gacttgcagc agcattcaat
    40261 gctcctatcg caggattact ctttgtttta gaagaaatcc atcactcctt ttctccactt
    40321 gtttggctga catcatttgc ttcagcaatc actgctaact tcgtctctct ttactttttc
    40381 ggcttgagac ctgttttata tctaggtgac cttccttctt tgccgttgaa atattatggt
    40441 tccttagtga tcttaggagt acttctaggc atcttaggtt ttatttatca gaatgtcttg
    40501 ctggctttgc caaaatttta caaccgattg cctttaccag cttattttca cgggttcgtt
    40561 cctttcttgc tgattttgcc aattggttat ttatggccta gtactctagg cggaggcaat
    40621 caaatcgttt tagcttttgg acatgcttcc ttgccaatca ttatgattat cggtttgttt
    40681 gttcttcgat tcgttttttc catgatttct tacggtgggg gtcttccagg ggggattttc
    40741 cttccgatat tgacattggg ggctttaatc ggtagctttt atggaaacat tatggttgat
    40801 ttcgggatgg atccaatcta catcaaggat tttgtcgtta ttgccatggc tggttatttc
    40861 acagcgattg gaaaagcacc gctgacagca attattttag tgacagaaat ggttggctct
    40921 cttaatcatc tgatgccttt aggattggct gctcttgttg cttatatcgt caatgacctt
    40981 ctcggcggta atccaattta cgaatcatta ttggaacgtc tacttggaaa tactcagacc
    41041 gcctcactaa aaggtgcaag gacaactttt gattttccag taacggcaga gagttcttta
    41101 gacggattaa tggtacgaga cttcaattgg cctaaaaata tgctgctaat ctctatacgc
    41161 agaggaaatc aggaaatctt gactcacggt gatacgatca tgaatgtagg agatattttg
    41221 gttatcttga ctgatgaagc aaacttgcca agaatcaagc aggaaatcga ggctaaatct
    41281 tctatcacaa atttgaagca atcattttaa cgaacatgga aaaactattt tacaacaact
    41341 attcatctac actacttgat tctatatgat tctataaaaa aagaagaggc tgggaaaacc
    41401 cagcctcttc ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca
    41461 taaatcacac cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga
    41521 ctcagtaaga atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct
    41581 ccgactaatc cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg
    41641 tcagctgctg caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc
    41701 gtcaaaccag catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg
    41761 aaaattgcca atggaggtac cattcctcca gccataacag aagccatcac gacacttccg
    41821 ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt
    41881 ggaccgccta aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc
    41941 gaaccactca tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca
    42001 ttgaccagca acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc
    42061 gttttaattc catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg
    42121 acataaccag ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta
    42181 gctaatgcac cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca
    42241 ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac
    42301 cagctagcag cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata
    42361 aattggtcga tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac
    42421 atgtgtgata cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca
    42481 tttccttctt ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta
    42541 agggctagat tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc
    42601 agttcttttc cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct
    42661 tctgcacggg cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt
    42721 tcgactttga tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg
    42781 taagtgtgag cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga
    42841 tgagcagaag cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc
    42901 tcttcagctg tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt
    42961 cctacgaaat ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg
    43021 tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag
    43081 gcttcataat ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca
    43141 ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg
    43201 cgggctagaa ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt
    43261 ttttggacca tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc
    43321 gcttctttga ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt
    43381 ttgtgatttc aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag
    43441 aaaaagctgt tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg
    43501 tacggctgta agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt
    43561 tcaatgggcg aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac
    43621 cgtctccagc cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat
    43681 acggaatgat ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat
    43741 tgtttggttt gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg
    43801 tatcaatcac gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc
    43861 cttttcgcag gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg
    43921 ttcctaatgc ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat
    43981 tgatctcagt ttcagtttct gattttagtt tgatattgat tcttgtatct tcttttacgg
    44041 ttgtgaacaa agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga
    44101 accctcctaa aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa
    44161 cattgatccc ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta
    44221 gttttaaccc atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa
    44281 tcattgtatt gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg
    44341 acaacgctcg gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg
    44401 gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg
    44461 tttaacagct gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt
    44521 cattcccata aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc
    44581 taaaacagca ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa
    44641 atcaactaaa tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg
    44701 cagaaaagga atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc
    44761 attaatggtt tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga
    44821 tttttcactc atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac
    44881 tctttctaga aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga
    44941 agcattcaaa aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc
    45001 taggattttt tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat
    45061 agcatatttt ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa
    45121 tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc
    45181 atcaagccgt tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca
    45241 ggttttcttt ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa
    45301 aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat
    45361 gatgccggaa ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt
    45421 ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg
    45481 ctcacctttc ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt
    45541 aacatcagag agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg
    45601 aacatagatt cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc
    45661 tagatattcc ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg
    45721 atcgataact ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc
    45781 ctgagcattt agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata
    45841 actttttgtt acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg
    45901 atactggaca gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa
    45961 taaaccgaca ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga
    46021 ttgaaacatt tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca
    46081 tatgacttcg ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg
    46141 tttagggatg atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca
    46201 taaaccttgg taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc
    46261 gttttgttgt ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg
    46321 ttgagagatc tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa
    46381 caccccatga gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt
    46441 gaaaacagag atagacaata cattttttat caataattga ttatcttcca agcgaaaact
    46501 acattcgttc tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat
    46561 tattttttct tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat
    46621 aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt
    46681 ttcttcttca tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat
    46741 tttctcacct ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta
    46801 aaactttatg tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac
    46861 aaagaggtgt ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc
    46921 gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt
    46981 ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc
    47041 aaaaaaagac ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt
    47101 ctttttatga ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg
    47161 taacggaaga aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt
    47221 cgctctcatg cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa
    47281 ttacagggat atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct
    47341 tatgacgcaa atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg
    47401 tctcgatcat ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc
    47461 ccggaacatc gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct
    47521 ttcctggctt accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag
    47581 aagatttccc tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat
    47641 attgttttgg tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc
    47701 tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg
    47761 gcagcctctt ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca
    47821 ccagacaatt cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg
    47881 atatcaaaca tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg
    47941 gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt
    48001 tcggtattat ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca
    48061 gtcaagatcc gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat
    48121 cgtccgataa actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc
    48181 atgacacttt cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg
    48241 cccatacgat tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat
    48301 atgttcgtcg tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt
    48361 ggcacacttg caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg
    48421 gaaacgtcac gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca
    48481 atatagatga tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta
    48541 agcaaaatgt tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct
    48601 gcgattgcaa aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca
    48661 gaccctgtag gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt
    48721 tcttgtgctt gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt
    48781 tttgcgcgat cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta
    48841 gggacttcag tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta
    48901 cataggtcga tacattcatt acagatgtag acccctggac cagctacgat ctttttcact
    48961 tcttcttggg ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca
    49021 tacatggtct tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca
    49081 gtcttttact atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact
    49141 gacctatacc tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg
    49201 tcacagtccc ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta
    49261 ttacttgatt tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt
    49321 ttcattgtca catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt
    49381 ggcatgttgt atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct
    49441 tgaagatttt cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct
    49501 gcttcacctt cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac
    49561 atttcaggag agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt
    49621 acttcatcat gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata
    49681 cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct
    49741 ttgcgatatt tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg
    49801 aattcgtcat ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag
    49861 actgcttctt ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca
    49921 tctagacttt cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag
    49981 cctgaaccta attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct
    50041 acaaagcctt cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg
    50101 ataactaatt cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct
    50161 gttacttcac ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct
    50221 ggtttcactg ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca
    50281 tcgatttttg gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct
    50341 tctggtaata cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg
    50401 aatacttgac gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact
    50461 ttattaaacg cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa
    50521 acaccatcat tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta
    50581 gaaattcatt ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct
    50641 ttcaaattgt aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt
    50701 ttcactgata aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt
    50761 aaaaaatctt gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct
    50821 acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca
    50881 attgcagatt gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc
    50941 ccgtaacgat ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc
    51001 agatcttttg gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacaggatgg
    51061 cattcgttca attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct
    51121 ggcgatacaa taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc
    51181 tcatcaatag acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg
    51241 tggagtgcct tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt
    51301 ttttcaatga cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta
    51361 gcatataaaa aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca
    51421 ttttgaagat actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc
    51481 aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa
    51541 gctcgttgaa ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc
    51601 actcgcatta aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct
    51661 cttgctcata ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa
    51721 ataactgaag caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa
    51781 ctgttactgc tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga
    51841 ttgatcgtaa caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta
    51901 aagactgctg gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca
    51961 ccttctctaa ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga
    52021 ataccaaaga tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa
    52081 tacgctagat aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa
    52141 ttaagccaac ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac
    52201 aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga
    52261 aaatcatttg cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt
    52321 ttctttttga tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg
    52381 gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta
    52441 ttttcatttg aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg
    52501 attccgctgt ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca
    52561 atccctggaa ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg
    52621 aagaatattc tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag
    52681 catcttcagg aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat
    52741 aaatccttcc atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga
    52801 cgattgctac aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt
    52861 cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg
    52921 tatcttccac acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac
    52981 caattgagcc ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat
    53041 agtagccacc gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt
    53101 tacgaatcgt atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag
    53161 aatttacttc taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt
    53221 ccataaaatt tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag
    53281 caatcgttcc atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca
    53341 catttggagc tagttcttca tccccatata accaactgtt caatccctca atttgatcgt
    53401 tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa
    53461 tcagaactgc tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta
    53521 tgacgatcac agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg
    53581 catacccgga aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag
    53641 caacttccaa ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata
    53701 ttttcgtatc atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca
    53761 ttcccagtgt catgatctca atttttgaat ccatttttac tcctcctcta tccgattact
    53821 ttctattgta ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata
    53881 aaaaggacat ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt
    53941 gacaagaaca ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga
    54001 ggatagttat accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt
    54061 ttatcgaaag attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa
    54121 tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat
    54181 tatagaaagt aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt
    54241 aacaccagga ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg
    54301 tatctttgtc tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa
    54361 tttgaaaaac aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg
    54421 ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg
    54481 cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt
    54541 agcaaatgac cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac
    54601 tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg
    54661 ttcttacgct ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa
    54721 taaaagtcca ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat
    54781 ggctatgctt gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt
    54841 cacatcagaa aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt
    54901 cgaagcacaa gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa
    54961 agaaatcgac gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga
    55021 caatcgtgtc tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta
    55081 tatcgttgct tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa
    55141 attgacacag attcctgttg aggttcattt agccagtgaa tttggctata acacacctct
    55201 tctttcaaaa aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg
    55261 ccaagtgctg gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc
    55321 tggttctact ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat
    55381 cgcagtagca tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa
    55441 agctatcggt gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt
    55501 gagtttagtt gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt
    55561 ggcagaagta tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta
    55621 caacgtttct cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg
    55681 ttttgccgct ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt
    55741 gatcggaatc atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt
    55801 cgaaagccgt ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga
    55861 ccagctggtg ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac
    55921 tcaattactt gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa
    55981 tttagcgaaa tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga
    56041 cactggtcct tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc
    56101 tttgactact gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc
    56161 tgccatcact cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga
    56221 tcccatttca gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc
    56281 gtttttatca cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc
    56341 aagctctgat aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag
    56401 catcccatct ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc
    56461 aaagttataa tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac
    56521 gacatctttc aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt
    56581 cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa
    56641 catgatttta tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc
    56701 atcgccatcg aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg
    56761 ttctggatgg gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat
    56821 tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc
    56881 gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca ccgttgatcg tttgtacaag
    56941 gaattgagaa tatttcaata agccttctgg aaattcttct acagttccca atccttcagc
    57001 tgatggacga ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg
    57061 gtcatctact agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg
    57121 attatgagaa gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc
    57181 aacacctggt gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc
    57241 tgaaatcagc gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac
    57301 gcgtgggcgt tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag
    57361 cttaaatgca agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc
    57421 aaaatattta cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt
    57481 ctgctcattt gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg
    57541 cttgtcccag attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca
    57601 gcagatgaaa tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca
    57661 gagctactgc ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg
    57721 atacttaacc cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct
    57781 gagacattga tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg
    57841 cttacttctg cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt
    57901 gttccttgct gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc
    57961 actgttactt gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc
    58021 tgtattggga cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct
    58081 actactcgtt tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga
    58141 ctaacggaag tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt
    58201 tccacatcga aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg
    58261 atctttgctg tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct
    58321 tcgcccagct tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct
    58381 gcattcaact gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca
    58441 tggatatagt agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga
    58501 ttatacgctt catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac
    58561 agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt
    58621 gagatcattt gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt
    58681 tcttgttttt tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt
    58741 gctgggataa gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt
    58801 cagagacaac gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc
    58861 gagtcccgaa ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta
    58921 ctgcgatctt tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa
    58981 agatattgat cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat
    59041 actcatctaa tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca
    59101 tatattggat ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt
    59161 ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct
    59221 gaaaaatcac gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca
    59281 aggtgtgcag accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt
    59341 tcagaagctg aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc
    59401 agacaaccag aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa
    59461 acatttgctg ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc
    59521 tctcttctct tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat
    59581 atgctcgtat ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt
    59641 tatttttctc tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg
    59701 ttatttttcc tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc
    59761 agctcttcta gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt
    59821 cttaacgggt acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc
    59881 tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa
    59941 taaagctttt gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct
    60001 catgaaatca ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt
    60061 gtttttttcg accattgtag ggttatatcg atataatgac caataaccag aagaaacagc
    60121 ttcttttgct tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg
    60181 cgtataggca ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat
    60241 cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat
    60301 cgccatcatt cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat
    60361 tgccgaagct ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc
    60421 caaaacaagg atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc
    60481 aatgtcgtaa gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc
    60541 ctttctcttg tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc
    60601 ttgcagtttt gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc
    60661 ttccatcaat tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc
    60721 caaagcctcg cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc
    60781 ttccaataaa gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga
    60841 agcaggagaa gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg
    60901 atcaccgaac atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc
    60961 gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt
    61021 gactggattt gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt
    61081 ctgttcttca taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca
    61141 cagaccacaa cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc
    61201 gcctcgcatt tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc
    61261 taaaaatgga cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca
    61321 gcgatcacta atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt
    61381 tcctaaaggc atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg
    61441 atttccttcc tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt
    61501 attgattttt tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag
    61561 cagttcggtc gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg
    61621 tccatagctt ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat
    61681 gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaa
//
LOCUS       DACEZN010000002        50832 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEZN010000002 DACEZN010000000
VERSION     DACEZN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.002"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..601)
                     /locus_tag="IXH60_000055"
     CDS             complement(<1..601)
                     /locus_tag="IXH60_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAQ1989029.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVYKD"
     gene            1220..4054
                     /locus_tag="IXH60_000056"
     CDS             1220..4054
                     /locus_tag="IXH60_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1989030.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            4245..4736
                     /locus_tag="IXH60_000057"
     CDS             4245..4736
                     /locus_tag="IXH60_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1989031.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            4839..5828
                     /gene="manX"
                     /locus_tag="IXH60_000058"
     CDS             4839..5828
                     /gene="manX"
                     /locus_tag="IXH60_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1989032.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5861..6664
                     /locus_tag="IXH60_000059"
     CDS             5861..6664
                     /locus_tag="IXH60_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1989033.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            6685..7599
                     /locus_tag="IXH60_000060"
     CDS             6685..7599
                     /locus_tag="IXH60_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1989034.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            7682..8056
                     /locus_tag="IXH60_000061"
     CDS             7682..8056
                     /locus_tag="IXH60_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1989035.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8195..8440
                     /locus_tag="IXH60_000062"
     CDS             8195..8440
                     /locus_tag="IXH60_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1989036.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8612..9676
                     /locus_tag="IXH60_000063"
     CDS             8612..9676
                     /locus_tag="IXH60_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1989037.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9922..10347
                     /locus_tag="IXH60_000064"
     CDS             9922..10347
                     /locus_tag="IXH60_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1989038.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10340..10789
                     /gene="nusB"
                     /locus_tag="IXH60_000065"
     CDS             10340..10789
                     /gene="nusB"
                     /locus_tag="IXH60_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1989039.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10886..11740
                     /locus_tag="IXH60_000066"
     CDS             10886..11740
                     /locus_tag="IXH60_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1989040.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            11741..13078
                     /locus_tag="IXH60_000067"
     CDS             11741..13078
                     /locus_tag="IXH60_000067"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1989041.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            13083..13307
                     /locus_tag="IXH60_000068"
     CDS             13083..13307
                     /locus_tag="IXH60_000068"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1989042.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            13309..14199
                     /locus_tag="IXH60_000069"
     CDS             13309..14199
                     /locus_tag="IXH60_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1989043.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            14208..15026
                     /locus_tag="IXH60_000070"
     CDS             14208..15026
                     /locus_tag="IXH60_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1989044.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            15133..15582
                     /locus_tag="IXH60_000071"
     CDS             15133..15582
                     /locus_tag="IXH60_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1989045.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            15600..17282
                     /gene="recN"
                     /locus_tag="IXH60_000072"
     CDS             15600..17282
                     /gene="recN"
                     /locus_tag="IXH60_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1989046.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(17337..17453)
                     /locus_tag="IXH60_000073"
     CDS             complement(17337..17453)
                     /locus_tag="IXH60_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1989047.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(17526..18482)
                     /locus_tag="IXH60_000074"
     CDS             complement(17526..18482)
                     /locus_tag="IXH60_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1989048.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            18653..19021
                     /locus_tag="IXH60_000075"
     CDS             18653..19021
                     /locus_tag="IXH60_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1989049.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            19191..19622
                     /gene="mraZ"
                     /locus_tag="IXH60_000076"
     CDS             19191..19622
                     /gene="mraZ"
                     /locus_tag="IXH60_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1989050.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            19643..20602
                     /gene="rsmH"
                     /locus_tag="IXH60_000077"
     CDS             19643..20602
                     /gene="rsmH"
                     /locus_tag="IXH60_000077"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1989051.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            20607..21008
                     /gene="ftsL"
                     /locus_tag="IXH60_000078"
     CDS             20607..21008
                     /gene="ftsL"
                     /locus_tag="IXH60_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1989052.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            21009..23201
                     /locus_tag="IXH60_000079"
     CDS             21009..23201
                     /locus_tag="IXH60_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1989053.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            23227..24189
                     /locus_tag="IXH60_000080"
     CDS             23227..24189
                     /locus_tag="IXH60_000080"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1989054.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            24203..25564
                     /locus_tag="IXH60_000081"
     CDS             24203..25564
                     /locus_tag="IXH60_000081"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1989055.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            25590..26678
                     /gene="murG"
                     /locus_tag="IXH60_000082"
     CDS             25590..26678
                     /gene="murG"
                     /locus_tag="IXH60_000082"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1989056.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            26693..27913
                     /locus_tag="IXH60_000083"
     CDS             26693..27913
                     /locus_tag="IXH60_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1989057.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            28069..29394
                     /gene="ftsA"
                     /locus_tag="IXH60_000084"
     CDS             28069..29394
                     /gene="ftsA"
                     /locus_tag="IXH60_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1989058.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            29417..30658
                     /gene="ftsZ"
                     /locus_tag="IXH60_000085"
     CDS             29417..30658
                     /gene="ftsZ"
                     /locus_tag="IXH60_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1989059.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            30675..31352
                     /locus_tag="IXH60_000086"
     CDS             30675..31352
                     /locus_tag="IXH60_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1989060.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            31366..31965
                     /locus_tag="IXH60_000087"
     CDS             31366..31965
                     /locus_tag="IXH60_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1989061.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31980..32267
                     /locus_tag="IXH60_000088"
     CDS             31980..32267
                     /locus_tag="IXH60_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1989062.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            32316..33098
                     /locus_tag="IXH60_000089"
     CDS             32316..33098
                     /locus_tag="IXH60_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1989063.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            33119..33826
                     /locus_tag="IXH60_000090"
     CDS             33119..33826
                     /locus_tag="IXH60_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1989064.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            34088..36886
                     /gene="ileS"
                     /locus_tag="IXH60_000091"
     CDS             34088..36886
                     /gene="ileS"
                     /locus_tag="IXH60_000091"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1989065.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36935..38461)
                     /locus_tag="IXH60_000092"
     CDS             complement(36935..38461)
                     /locus_tag="IXH60_000092"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1989066.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(38476..39123)
                     /locus_tag="IXH60_000093"
     CDS             complement(38476..39123)
                     /locus_tag="IXH60_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1989067.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(39307..39636)
                     /locus_tag="IXH60_000094"
     CDS             complement(39307..39636)
                     /locus_tag="IXH60_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1989068.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            39813..40541
                     /gene="sfsA"
                     /locus_tag="IXH60_000095"
     CDS             39813..40541
                     /gene="sfsA"
                     /locus_tag="IXH60_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1989069.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            40557..41570
                     /locus_tag="IXH60_000096"
     CDS             40557..41570
                     /locus_tag="IXH60_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1989070.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            41570..42847
                     /locus_tag="IXH60_000097"
     CDS             41570..42847
                     /locus_tag="IXH60_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1989071.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42910..45612
                     /locus_tag="IXH60_000098"
     CDS             42910..45612
                     /locus_tag="IXH60_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1989072.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            45764..46081
                     /locus_tag="IXH60_000099"
     CDS             45764..46081
                     /locus_tag="IXH60_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1989073.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            46111..46431
                     /locus_tag="IXH60_000100"
     CDS             46111..46431
                     /locus_tag="IXH60_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1989074.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            46539..47999
                     /locus_tag="IXH60_000101"
     CDS             46539..47999
                     /locus_tag="IXH60_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1989075.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            48067..48288
                     /locus_tag="IXH60_000102"
     CDS             48067..48288
                     /locus_tag="IXH60_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989076.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            48319..48501
                     /locus_tag="IXH60_000103"
     CDS             48319..48501
                     /locus_tag="IXH60_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1989077.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            48501..48914
                     /locus_tag="IXH60_000104"
     CDS             48501..48914
                     /locus_tag="IXH60_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1989078.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            49037..50218
                     /locus_tag="IXH60_000105"
     CDS             49037..50218
                     /locus_tag="IXH60_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1989079.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            50607..50680
                     /locus_tag="IXH60_000106"
     tRNA            50607..50680
                     /locus_tag="IXH60_000106"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50641..50643,aa:Arg,seq:cct)
     gene            complement(50749..>50832)
                     /locus_tag="IXH60_000107"
     CDS             complement(50749..>50832)
                     /locus_tag="IXH60_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ1989080.1"
                     /translation="LHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        16898 a         8594 c        10902 g        14438 t
ORIGIN      
        1 tatctttata aactgcaaca ttcaatactt taccaagagt atcaatagtt aatgtaccca
       61 acagcactcc tcttttttta ctaatattat ttaaattgtc taatataata ctttgtacat
      121 tattgcctat cattttttgt tgagctacaa ttaaacgttt attagttaat gcgtatgcat
      181 aattattgtc atgcttagtt gatgagatat aattatgcaa tcctacaaag cagaagatta
      241 cgtattcgtc actgttaagt tgatctgata ttaagttaaa atgatgagtt gtccattttt
      301 tagtcattcc tttaccgtag ccgtatttaa gacaaaattc taccatctct tcagtggttc
      361 tcattctatc attacctata tcatgattgt tttgtgcagt atgatcagat atgtttaatt
      421 ttcttttttc tgatggatcc ttatctaaat cttctaaatt caaagatctt attattttat
      481 aaggagtagt cataccgtaa ccagcttttt tcatacaatt gccgcaaaca atttgtttat
      541 ctttgatttt atatgaagtt aagccaattt tacctccgca ctttgcacat tgtttactca
      601 cattagttcc tcctagatat cagctttcta ctactattaa cattctatca atagtgacca
      661 tactttgacc atacttttta gaaagaatag aaaattccaa gatatttaat aatagaagaa
      721 aagcactaac atcatgggtt cagaagaatt gagagcttat caggaaacag tataattgaa
      781 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
      841 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
      901 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
      961 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
     1021 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
     1081 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
     1141 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
     1201 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
     1261 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
     1321 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
     1381 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
     1441 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
     1501 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
     1561 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
     1621 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
     1681 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
     1741 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
     1801 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
     1861 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
     1921 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
     1981 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
     2041 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
     2101 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
     2161 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
     2221 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
     2281 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
     2341 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
     2401 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
     2461 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
     2521 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
     2581 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
     2641 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
     2701 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
     2761 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
     2821 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
     2881 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
     2941 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
     3001 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
     3061 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
     3121 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
     3181 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
     3241 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
     3301 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
     3361 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
     3421 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
     3481 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
     3541 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
     3601 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
     3661 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
     3721 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
     3781 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
     3841 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
     3901 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
     3961 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
     4021 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
     4081 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
     4141 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
     4201 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
     4261 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
     4321 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
     4381 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
     4441 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
     4501 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
     4561 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
     4621 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
     4681 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
     4741 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
     4801 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
     4861 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
     4921 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
     4981 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
     5041 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
     5101 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
     5161 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
     5221 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
     5281 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
     5341 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
     5401 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
     5461 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
     5521 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
     5581 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
     5641 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
     5701 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
     5761 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
     5821 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
     5881 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
     5941 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
     6001 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
     6061 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
     6121 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
     6181 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
     6241 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
     6301 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
     6361 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
     6421 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
     6481 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
     6541 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
     6601 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
     6661 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
     6721 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
     6781 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
     6841 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
     6901 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
     6961 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
     7021 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
     7081 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
     7141 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
     7201 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
     7261 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
     7321 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
     7381 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
     7441 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
     7501 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
     7561 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
     7621 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
     7681 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
     7741 tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt accatgcgcg
     7801 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
     7861 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
     7921 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
     7981 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
     8041 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
     8101 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
     8161 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
     8221 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
     8281 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
     8341 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
     8401 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
     8461 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
     8521 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
     8581 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
     8641 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
     8701 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
     8761 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
     8821 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
     8881 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
     8941 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
     9001 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
     9061 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
     9121 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
     9181 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
     9241 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
     9301 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
     9361 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
     9421 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
     9481 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
     9541 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
     9601 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
     9661 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
     9721 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
     9781 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
     9841 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
     9901 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
     9961 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    10021 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    10081 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    10141 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    10201 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    10261 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    10321 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    10381 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    10441 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    10501 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    10561 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    10621 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    10681 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    10741 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    10801 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    10861 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    10921 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    10981 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    11041 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    11101 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    11161 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    11221 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    11281 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    11341 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    11401 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    11461 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    11521 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    11581 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    11641 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    11701 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    11761 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    11821 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    11881 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    11941 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    12001 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    12061 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    12121 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    12181 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    12241 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    12301 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    12361 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    12421 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    12481 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    12541 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    12601 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    12661 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    12721 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    12781 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    12841 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    12901 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    12961 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    13021 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    13081 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    13141 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    13201 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    13261 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    13321 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    13381 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    13441 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    13501 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    13561 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    13621 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    13681 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    13741 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    13801 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    13861 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    13921 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    13981 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    14041 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    14101 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    14161 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    14221 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    14281 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    14341 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    14401 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    14461 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    14521 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    14581 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    14641 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    14701 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    14761 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    14821 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    14881 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    14941 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    15001 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    15061 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    15121 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    15181 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    15241 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    15301 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    15361 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    15421 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    15481 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    15541 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    15601 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    15661 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    15721 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    15781 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    15841 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    15901 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    15961 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    16021 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    16081 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    16141 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    16201 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    16261 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    16321 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    16381 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    16441 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    16501 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    16561 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    16621 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    16681 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    16741 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    16801 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    16861 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    16921 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    16981 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    17041 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    17101 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    17161 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    17221 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    17281 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    17341 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    17401 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    17461 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    17521 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    17581 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    17641 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    17701 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    17761 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    17821 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    17881 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    17941 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    18001 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    18061 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    18121 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    18181 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    18241 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    18301 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    18361 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    18421 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    18481 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    18541 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    18601 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    18661 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    18721 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    18781 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    18841 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    18901 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    18961 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    19021 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    19081 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    19141 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    19201 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    19261 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    19321 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    19381 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    19441 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    19501 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    19561 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    19621 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    19681 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    19741 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    19801 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    19861 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    19921 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    19981 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    20041 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    20101 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    20161 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    20221 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    20281 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    20341 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    20401 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    20461 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    20521 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    20581 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    20641 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    20701 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    20761 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    20821 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    20881 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    20941 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    21001 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    21061 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    21121 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    21181 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    21241 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    21301 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    21361 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    21421 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    21481 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    21541 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    21601 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    21661 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    21721 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    21781 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    21841 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    21901 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    21961 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    22021 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    22081 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    22141 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    22201 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    22261 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    22321 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    22381 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    22441 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    22501 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    22561 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    22621 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    22681 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    22741 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    22801 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    22861 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    22921 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    22981 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    23041 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    23101 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    23161 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    23221 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    23281 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    23341 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    23401 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    23461 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    23521 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    23581 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    23641 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    23701 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    23761 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    23821 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    23881 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    23941 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    24001 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    24061 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    24121 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    24181 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    24241 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    24301 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    24361 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    24421 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    24481 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    24541 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    24601 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    24661 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    24721 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    24781 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    24841 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    24901 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    24961 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    25021 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    25081 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    25141 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    25201 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    25261 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    25321 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    25381 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    25441 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    25501 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    25561 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    25621 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    25681 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    25741 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    25801 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    25861 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    25921 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    25981 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    26041 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    26101 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    26161 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    26221 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    26281 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    26341 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    26401 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    26461 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    26521 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    26581 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    26641 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    26701 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    26761 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    26821 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    26881 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    26941 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    27001 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    27061 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    27121 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    27181 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    27241 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    27301 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    27361 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    27421 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    27481 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    27541 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    27601 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    27661 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    27721 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    27781 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    27841 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    27901 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    27961 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    28021 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    28081 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    28141 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    28201 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    28261 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    28321 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    28381 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    28441 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    28501 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    28561 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    28621 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    28681 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    28741 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    28801 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    28861 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    28921 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    28981 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    29041 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    29101 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    29161 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    29221 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    29281 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    29341 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    29401 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    29461 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    29521 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    29581 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    29641 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    29701 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    29761 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    29821 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    29881 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    29941 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    30001 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    30061 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    30121 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    30181 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    30241 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    30301 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    30361 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    30421 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    30481 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    30541 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    30601 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    30661 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    30721 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    30781 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    30841 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    30901 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    30961 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    31021 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    31081 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    31141 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    31201 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    31261 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    31321 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    31381 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    31441 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    31501 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    31561 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    31621 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    31681 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    31741 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    31801 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    31861 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    31921 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    31981 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    32041 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    32101 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    32161 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    32221 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    32281 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    32341 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    32401 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    32461 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    32521 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    32581 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    32641 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    32701 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    32761 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    32821 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    32881 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    32941 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    33001 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    33061 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    33121 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    33181 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    33241 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    33301 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    33361 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    33421 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    33481 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    33541 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    33601 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    33661 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    33721 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    33781 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    33841 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    33901 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    33961 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    34021 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    34081 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    34141 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    34201 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    34261 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    34321 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    34381 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    34441 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    34501 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    34561 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    34621 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    34681 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    34741 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    34801 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    34861 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    34921 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    34981 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    35041 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    35101 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    35161 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    35221 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    35281 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    35341 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    35401 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    35461 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    35521 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    35581 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    35641 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    35701 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    35761 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    35821 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    35881 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    35941 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    36001 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    36061 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    36121 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    36181 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    36241 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    36301 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    36361 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    36421 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    36481 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    36541 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    36601 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    36661 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    36721 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    36781 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    36841 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    36901 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    36961 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    37021 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    37081 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    37141 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    37201 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    37261 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    37321 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    37381 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    37441 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    37501 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    37561 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    37621 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    37681 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    37741 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    37801 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    37861 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    37921 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    37981 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    38041 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    38101 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    38161 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    38221 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    38281 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    38341 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    38401 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    38461 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    38521 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    38581 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    38641 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    38701 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    38761 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    38821 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    38881 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    38941 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    39001 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    39061 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    39121 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    39181 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    39241 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    39301 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    39361 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    39421 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    39481 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    39541 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    39601 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    39661 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    39721 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    39781 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    39841 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    39901 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    39961 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    40021 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    40081 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    40141 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    40201 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    40261 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    40321 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    40381 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    40441 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    40501 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    40561 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    40621 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    40681 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    40741 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    40801 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    40861 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    40921 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    40981 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    41041 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    41101 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    41161 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    41221 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    41281 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    41341 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    41401 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    41461 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    41521 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    41581 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    41641 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    41701 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    41761 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    41821 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    41881 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    41941 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    42001 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    42061 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    42121 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    42181 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    42241 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    42301 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    42361 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    42421 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    42481 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    42541 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    42601 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    42661 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    42721 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    42781 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    42841 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    42901 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    42961 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    43021 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    43081 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    43141 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    43201 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    43261 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    43321 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
    43381 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    43441 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    43501 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    43561 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    43621 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    43681 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    43741 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    43801 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    43861 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    43921 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    43981 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    44041 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    44101 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    44161 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    44221 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    44281 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    44341 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    44401 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    44461 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    44521 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    44581 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    44641 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    44701 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    44761 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    44821 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    44881 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    44941 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    45001 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    45061 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    45121 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    45181 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    45241 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    45301 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    45361 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    45421 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    45481 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    45541 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    45601 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    45661 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    45721 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    45781 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    45841 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    45901 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    45961 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    46021 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    46081 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    46141 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    46201 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    46261 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    46321 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    46381 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    46441 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    46501 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    46561 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    46621 tgcgtgatgc ttttatcggc acactgcccg caacaatggc gggatcagtc gctgtaatga
    46681 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    46741 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    46801 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    46861 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    46921 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    46981 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    47041 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    47101 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    47161 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    47221 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    47281 ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc
    47341 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    47401 ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    47461 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    47521 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    47581 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    47641 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    47701 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    47761 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    47821 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    47881 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    47941 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    48001 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tctaggagga
    48061 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    48121 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    48181 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    48241 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    48301 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    48361 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    48421 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    48481 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    48541 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    48601 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    48661 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    48721 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    48781 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    48841 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    48901 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    48961 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    49021 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    49081 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    49141 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    49201 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    49261 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    49321 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    49381 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    49441 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    49501 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    49561 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    49621 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    49681 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
    49741 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
    49801 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
    49861 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
    49921 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
    49981 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
    50041 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
    50101 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
    50161 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
    50221 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
    50281 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
    50341 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
    50401 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
    50461 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
    50521 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
    50581 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
    50641 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc
    50701 tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt
    50761 tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg
    50821 aacaatatga ag
//
LOCUS       DACEZN010000003        46411 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEZN010000003 DACEZN010000000
VERSION     DACEZN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.003"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..335
                     /locus_tag="IXH60_000108"
     CDS             <1..335
                     /locus_tag="IXH60_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1989081.1"
                     /translation="ELTEEGKEQLAEWIHTPTNELPVNRDEFVLKLYFVKDVNDPALV
                     EIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAHAINRETEYANWLTDV
                     LEESNRKK"
     gene            complement(488..961)
                     /locus_tag="IXH60_000109"
     CDS             complement(488..961)
                     /locus_tag="IXH60_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1989082.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            1099..2571
                     /locus_tag="IXH60_000110"
     CDS             1099..2571
                     /locus_tag="IXH60_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1989083.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2568..3278
                     /locus_tag="IXH60_000111"
     CDS             2568..3278
                     /locus_tag="IXH60_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1989084.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            3630..3914
                     /locus_tag="IXH60_000112"
     CDS             3630..3914
                     /locus_tag="IXH60_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1989085.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(4195..4614)
                     /locus_tag="IXH60_000113"
     CDS             complement(4195..4614)
                     /locus_tag="IXH60_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1989086.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(4616..5200)
                     /locus_tag="IXH60_000114"
     CDS             complement(4616..5200)
                     /locus_tag="IXH60_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1989087.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(5247..6722)
                     /locus_tag="IXH60_000115"
     CDS             complement(5247..6722)
                     /locus_tag="IXH60_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1989088.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            6903..7214
                     /locus_tag="IXH60_000116"
     CDS             6903..7214
                     /locus_tag="IXH60_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989089.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(7329..9665)
                     /locus_tag="IXH60_000117"
     CDS             complement(7329..9665)
                     /locus_tag="IXH60_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1989090.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            9807..10475
                     /locus_tag="IXH60_000118"
     CDS             9807..10475
                     /locus_tag="IXH60_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1989091.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            10478..11521
                     /locus_tag="IXH60_000119"
     CDS             10478..11521
                     /locus_tag="IXH60_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1989092.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(11758..12639)
                     /locus_tag="IXH60_000120"
     CDS             complement(11758..12639)
                     /locus_tag="IXH60_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1989093.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            12798..13526
                     /locus_tag="IXH60_000121"
     CDS             12798..13526
                     /locus_tag="IXH60_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1989094.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            13682..13999
                     /locus_tag="IXH60_000122"
     CDS             13682..13999
                     /locus_tag="IXH60_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1989095.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            13992..14600
                     /locus_tag="IXH60_000123"
     CDS             13992..14600
                     /locus_tag="IXH60_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1989096.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            14616..15446
                     /locus_tag="IXH60_000124"
     CDS             14616..15446
                     /locus_tag="IXH60_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1989097.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            15584..16072
                     /locus_tag="IXH60_000125"
     CDS             15584..16072
                     /locus_tag="IXH60_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1989098.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            16123..16995
                     /locus_tag="IXH60_000126"
     CDS             16123..16995
                     /locus_tag="IXH60_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1989099.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            17171..17533
                     /locus_tag="IXH60_000127"
     CDS             17171..17533
                     /locus_tag="IXH60_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1989100.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            17542..18294
                     /locus_tag="IXH60_000128"
     CDS             17542..18294
                     /locus_tag="IXH60_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989101.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDVGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            18308..20110
                     /locus_tag="IXH60_000129"
     CDS             18308..20110
                     /locus_tag="IXH60_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989102.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            20387..21427
                     /locus_tag="IXH60_000130"
     CDS             20387..21427
                     /locus_tag="IXH60_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1989103.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(21479..23623)
                     /locus_tag="IXH60_000131"
     CDS             complement(21479..23623)
                     /locus_tag="IXH60_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1989104.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23980..25353)
                     /locus_tag="IXH60_000132"
     CDS             complement(23980..25353)
                     /locus_tag="IXH60_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1989105.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(25353..25748)
                     /gene="rbsD"
                     /locus_tag="IXH60_000133"
     CDS             complement(25353..25748)
                     /gene="rbsD"
                     /locus_tag="IXH60_000133"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1989106.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(25776..26693)
                     /gene="rbsK"
                     /locus_tag="IXH60_000134"
     CDS             complement(25776..26693)
                     /gene="rbsK"
                     /locus_tag="IXH60_000134"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1989107.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(26801..27814)
                     /locus_tag="IXH60_000135"
     CDS             complement(26801..27814)
                     /locus_tag="IXH60_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1989108.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(27895..28494)
                     /locus_tag="IXH60_000136"
     CDS             complement(27895..28494)
                     /locus_tag="IXH60_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1989109.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            28680..29381
                     /locus_tag="IXH60_000137"
     CDS             28680..29381
                     /locus_tag="IXH60_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989110.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            29391..32102
                     /locus_tag="IXH60_000138"
     CDS             29391..32102
                     /locus_tag="IXH60_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1989111.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEGL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            32553..34181
                     /gene="treC"
                     /locus_tag="IXH60_000139"
     CDS             32553..34181
                     /gene="treC"
                     /locus_tag="IXH60_000139"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1989112.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(34241..35422)
                     /locus_tag="IXH60_000140"
     CDS             complement(34241..35422)
                     /locus_tag="IXH60_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1989113.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            35674..37197
                     /locus_tag="IXH60_000141"
     CDS             35674..37197
                     /locus_tag="IXH60_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1989114.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(37266..37994)
                     /locus_tag="IXH60_000142"
     CDS             complement(37266..37994)
                     /locus_tag="IXH60_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1989115.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(38007..39287)
                     /locus_tag="IXH60_000143"
     CDS             complement(38007..39287)
                     /locus_tag="IXH60_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1989116.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(39428..40030)
                     /locus_tag="IXH60_000144"
     CDS             complement(39428..40030)
                     /locus_tag="IXH60_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1989117.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            40275..40481
                     /locus_tag="IXH60_000145"
     CDS             40275..40481
                     /locus_tag="IXH60_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989118.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            40793..41512
                     /locus_tag="IXH60_000146"
     CDS             40793..41512
                     /locus_tag="IXH60_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1989119.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(41593..42276)
                     /locus_tag="IXH60_000147"
     CDS             complement(41593..42276)
                     /locus_tag="IXH60_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1989120.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            42494..44680
                     /locus_tag="IXH60_000148"
     CDS             42494..44680
                     /locus_tag="IXH60_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1989121.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            45026..45436
                     /locus_tag="IXH60_000149"
     CDS             45026..45436
                     /locus_tag="IXH60_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1989122.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            45450..45806
                     /locus_tag="IXH60_000150"
     CDS             45450..45806
                     /locus_tag="IXH60_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1989123.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        15092 a         8174 c         9283 g        13862 t
ORIGIN      
        1 atgaattaac agaagaaggg aaggaacaat tggcagagtg gattcataca cccaccaatg
       61 aactaccggt taatcgagac gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg
      121 atccggcatt agtagaaatt attcagcagc agcgtgacct tcatgaagaa aaacgacttc
      181 atctattaga gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat gaaaattacg
      241 gacactacct gattttagcg catgcgatca atcgggaaac agaatatgca aattggctaa
      301 cagatgtatt agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga
      361 attcctttct atatgaaaga aacgcagatc accgttcgga tattcttatg ttcaaacagg
      421 ccttttttca agtatttatg tagcatctac ttattaaaaa gcttggtact taggagatta
      481 gtggcgttta gagttctttt aacaactgtt cgtagtcagc tttttcatcg acaggaagga
      541 gcttgtaagt taattcgtct gtctcgttgt catgaatgat gtacatctta gcattctctt
      601 ctttgaagtt accgatttgt aaaatcgtat agcgttcgtc gatcaattcg atatctttga
      661 ttgcttttga aaaaacttta cggacatggt gcattttctt ttggcggatt tctgcctttt
      721 ctgcagcttt tgacaaagta aatccgtcat ctaaatattt tttgatcagt tctactttta
      781 tgactgtagg taaacgatat tttctaggac aattcgcatc agcttcgatc gaacgaatga
      841 atcccttttg ttcccaatac cttaattgac gtggagacac acctacgata tcacttaatt
      901 cactgatgcc aacaaccaat tgatcatttt ccaacaaacg tttaagggaa gccccaacca
      961 tttttgtcac tccttttgtc ctcttgcttc ccttaattat atccgtatta acttatgtgt
     1021 caagtaggtt gacaaatagc gaatttagga ctaaagtatt ctagtagttt cagaaaaaat
     1081 tcagaaagaa ggagaaatat gagtaacaac caacccgtag atatttacgg aaaaccatat
     1141 aatcggacac ttttagttgt tgtcttatta attggtacat tttgtaccgt tttgaatcag
     1201 accttattga cgacagcttt tcctacattg atgaaagctt tcgatatttc agcgtctgat
     1261 gtgcaatggt taacgactgg tttcttgcta gtcaacggga tcatgatccc aataacagca
     1321 tggctgatca ataaattcag ttcaagaaat ttatatcttt ctgccatgac gatctttttg
     1381 atcggaacga ttacttgctt tacagcacct aattttagta cattactaat cggtcgtttg
     1441 atccaagctt gcggtgtggg agtatcgatg ccattattgc aaaatatcat gctatctatt
     1501 ttcccgccag aaaaaagagg atcagcaatg gggatgtcag ggatcgttat tggacttgct
     1561 cctgctttag gccctacatt atctggttat atcattgata actaccattg gcgtgacttg
     1621 tttggtatgg tactgccaat cgttgttctt gtcttggttt tagcattctt cctaatgaaa
     1681 agtgttattc cattgtcaaa tcctagcatt gatattttat ccgctatttt atcaacgatt
     1741 ggattcggta gtttacttta cggattttct agtgtaggag atgacggctg gggaagcttg
     1801 aaagtaattg gtttcttagt tgttggtgtg attttcatcg ccttgttctc atggcgtcaa
     1861 ttacatttgg aacatccatt cttggaactt cgtgtattca aatcatctac attcacgatt
     1921 gcagctgttt tagctggtgt aacaaatatg gcgatggtag gggcagaaat ggtcgtacca
     1981 ttgtatattc aaaatattcg tggagaatca gcattccatt ctggcttgat gttgttgccg
     2041 ggtgcattga tcatgggggc tatgatgcct gtgacgggtg cgattttcga taaatacggc
     2101 gcgaaacgtt tagccatcac agggatgttc attttgacag cggcaactgc accatttgct
     2161 ttcttgacaa aaacgactcc agttttatat atcgtgatcc tttatgcgat ccgtatgttt
     2221 gggatctcca tggtcatgat gccggtaacg acttctggta tgaatgcatt gccaaataac
     2281 ttgatcagtc atgggacagc ggtaaataat acattccgtc aaatcgcaag ctcgattggt
     2341 acagctattt tgatcagtgt gttgacgaat gtgacaaaag caaatatccc aagcaaatct
     2401 gtattggata atacgccatt agcttataaa gatcaggcaa tcaatgccac actgtcaggt
     2461 taccatgccg cgtttatcgt agcgatcatc tttgggattc ttggctttat tattgccttt
     2521 ttattgaaga aaaaagaacc tgttctaatg aaagcggggg ataatgcatg attattggag
     2581 ttttgattgt cagcttgctt gcttttgctt taacgaacgt gttcgctaaa aaaacatggc
     2641 aaaccttttt atctttgatt ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg
     2701 ctaatttgtc caatcacttt ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg
     2761 tttccagtgc tgattcccaa ggagcagata tgcttttgta taaagcttta gggaatggaa
     2821 aagaaaaagt gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta
     2881 cggataacga aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa
     2941 ctacttattg ggaatacaaa aacgacatgt ataaattctg gttcaatatt gctgataaca
     3001 atcacgaata tgacagccga gtgaatacat tcaaaatccc agagacatgg gtagaattaa
     3061 gtacggacca agcagccaag ttagctgaat tagtgaagaa acagcaaagt acgatggaaa
     3121 gtgaagcaaa agcttatgtt caagacggta tggtaaaagc aatgactgaa aatccaaaaa
     3181 tgagcaaagc agagcaagaa caacgtacga aagccttagc tgctgaattc caacaacaag
     3241 cattcgcaaa attagtgaaa gaagcaaaag gcgaataata ttttgaacac catgtaggag
     3301 gttgtgaaaa agctgctctg catcaagcag taagaacaat cagataaaaa tagcactcca
     3361 tatttttcat ctgattgggc ttagtgccgt agatgcaagc ttttgaacac cgtttaagga
     3421 agaggttgtg agcctgccgt ttaactctga gtcacaagga gcaatttcaa aaaacagctt
     3481 ctcatgtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat
     3541 tcgaaaaaag gaactgcgac aagacttttg tcacagttcc ttcttttttt tgctatgcta
     3601 tactagatgt aaaaaatagg agaaataaaa tgagggaaat ttattctttt gaatcaaaaa
     3661 tctatgcttc cgaagtcggc aagggcgggg catatattat ttttccgtat gatataagaa
     3721 aagaattcgg tgctggaaga gttaaagtcc aagcaacttt tgacaatgtg ccgtatgaag
     3781 gcagtatcgt gaatatgggc gtgaaaaatc cagatggttc agtctgctac atccttggac
     3841 tacgaaaaga tattcgtaaa aacatcggaa aagaaatcgg tgatattgta gcagttacag
     3901 tgaagcaaaa ataaaagagg ttgtgacaaa agacgtgtca caacctcttg ataaacggtg
     3961 ttcaaaagcc agtttctcgg tagtaagtca aatttatgca aaaatgtgga aacccatgtt
     4021 tacaaaattt ttcttactac tcgaaacgaa gcggctttat cacaacctct tgataaacgg
     4081 cgttcaatca gctgtccctc ggtattagct caaaaacggc aaaatatgag aagctatttt
     4141 tccatttttt tctctcatac tcaggtcata acgctgattt cacaacctct tctttcataa
     4201 tggtttttca ccatctttat aataaactgg ttctttgata tggaaatcta ttttttccaa
     4261 ccaatctaaa atcttgtaac ctaactcttc tgacatcgga aaagaggcgt tgatttgtgc
     4321 attggagata tggtcaacat ggataccgga agtgatcgta caacttcccg gaacggcagt
     4381 tttgatgatc acagctattt tttcggctaa gacatcgtct ttatggaacc gaccatcgtg
     4441 gctagggaaa cgtacagttt gcatctctgt atcacgagaa aatgtagtga ctgttccgat
     4501 atgtggtgta tctccgcctg ttaaagtgat caataagtct ttaccgatta ctttaacagc
     4561 agcttggatc gtgaaaccta gtttggttac ttcaactatc gtttcttttt ccatattaat
     4621 caccctgcca acgttttgaa tgctcattta caatttcaaa ttgatccaaa agtttcattg
     4681 tatggtgatt aacaagatct tggatcgttt gaggctgatt ataaaaagca ggaattggtg
     4741 gtatgatttg cacacctagt cgtgctaatt tagtcaagtt ttccaagtga atggcattta
     4801 atggcgtttc acgaggaaca atgactaact ttcgttgctc cttcattgtt acatcagcag
     4861 cacgtgcaat caggttgtcg cctaaaccaa cagagatacc tgcgactgtt ttcatacttg
     4921 ccggtacgat gatcattcca tcggtcaaaa atgatccgct ggcgacagca gcacctaaat
     4981 ctttgtttga ataatggtaa tcaaacaggc tttcgaattc agttagtgat aagtcggatt
     5041 ccagctttaa gttctgtttt gcccatggac tgatgattcc gtgtgtttct atcatcggat
     5101 attcttttag tttcttcact aggttgatgg cataaatggt gcctgaagcg ccacttacac
     5161 caatcacgat ttttttcttc cgttgtgcca attgttccat tttccgcact cctttttatg
     5221 atgtcaaaac agttgcagat cattcattat ttcaaatatt tttcccaatc ttctacttct
     5281 tggaattttg cacgtacaaa atgttctttc atatcgaaag gaactgtacc gtcaaagata
     5341 gtttttgcac ttatcccacg gaaacgaata gagccaggat tatattctgg taattctgat
     5401 gggtccaatg gatggttcct cattcctggg atcacgatga tatctttatc tccttggaaa
     5461 cgagtgttga aagtccaaac tacatcgttc atgtcgaaaa tatcaacatc ttcatcgact
     5521 aaaatgactg tcttcaattc tttgaaggct gataaagcaa gaatagctgc ttgacgctga
     5581 ataccttcat ccgcttcgtt ttctttacgg atttgcatga tggtcatcaa tttaccacca
     5641 cctgcaggag gattgtagac atttactact tttcctggaa tagctttatc gaccaaggca
     5701 agaatacttg cttctgtagg aataccggcc atagatacgt gttcttcact gggaccaatc
     5761 gttgtttgca tgattggatt gtcttttcgg tgagtaactg ctttgacttt cactacttgt
     5821 acagctggat tagcatcccc agtatagcca ggaaattctg gcattgcttt tcctgagttc
     5881 gtgttgatat cttcttgcat tgtttcattt ggcatgattt ctgcttcaat gatgaattca
     5941 gcacgagcaa tacccacttc atcgactgct ataccatcaa cgatttgaat aggttcgtta
     6001 cgcaaagctc ctgcacacca tagttcattg tagccaagtg gagtggtagg tggttcgaaa
     6061 gtcgtgccaa tggcaattgc tggatctaaa ccaatattga tagtgatagg cattggttta
     6121 ttcaaggctt cgaattgttt ttggaagtga ccaatgtggc gtccgccagg catgatgtac
     6181 ataccgatcg tatctttatc ttctagaacc atacgatgga tcgttacatc gctcattgtt
     6241 ttttctggat ctgatcctaa gactacaccc attgtgatgt atggaccagc atcttcagga
     6301 gtatttgtag gtgcaggcaa aatgttgcga atatcgaagt ctgcatcggt agctagatga
     6361 acgacttctt gtgctggagc gtctgctttg tctactttca caggtttgat tggattttct
     6421 acagcgtctt tcaagaaacg accaagtgtt cgataatcat ggtgcaaaat ttcacctaca
     6481 cgtttacgac tagccattgt tccgatgttc acacgtgtgc cggggaaacc tttgatattg
     6541 ttgaaagtca tagctggtcc ttcttgagtc ggacgtttta ctgtaccgcc agcaccgata
     6601 taacggtaaa caccagataa ttctgcttca ggatcgattt caatatctgt ttcgtgatat
     6661 tgacctggaa gtgtttttag ttcttcgatg acttttctta aatcatacgg ttgatctgtc
     6721 atgagagatt cctccttagt tgtctttacc tatctatagt aaaaggtgaa aaagaggaaa
     6781 acaattcaac ttgttcggta aagtattcta caatggaatg gatgcgtttt tttgataata
     6841 gaataaacta tgatatactt atcactaaaa gaaagcgcat acttagtgtt ggaggaagga
     6901 aagtgaaaaa aatggaggag tcatttccta aagcagtaaa agtggaaaat atagcgaata
     6961 ttttgaaagt tacatttgaa aacggagaag taaaatacgt gaaatctcat tggacagaag
     7021 agataaccga tgccttgcaa tttggaaaaa aaggaagagg aaaaagaaaa aatcttctcg
     7081 cactttctcg taatatgtgg attgggactg aagtaacgat tgaagcagat ggaacagtat
     7141 tcataaatgg aaaagacagg tatacgccag aagagttgtg gtacaaagga aagaaaagta
     7201 taccagaatt ataaaaaata attaaaaaaa acagcgcgaa acaaggaaca tattacttcc
     7261 tcgtttcttg ctgttttcta ctatttcagc gctcttatga ctcaaatcta aatgtggttg
     7321 tcacaacctt attctgcacg caaagcgata gcggcgtctt ttttcgctgc cattcgtgca
     7381 ggaagatgac cgccaagcat cgtcaatata gtagagacga tcactagaat aatcgcatgg
     7441 acaggattta gttgagcaac attttttaga tccgtcatgt tgtataagat gctattgata
     7501 gggaacgtag ctagccatgc aataaacaca cctaatatcc cagaagaaat acctaaaata
     7561 cacgtttctg cgtcaaatac gcgagtgatg tcttttttac gtgcacctaa agcttttagt
     7621 acaccgattt cttttgtccg ttcaatcaca gatgtataag taatgatact gatcatgatc
     7681 atacttgtta ctaaagagat ccctgcaaaa gcaataagga cataagtgat agcatccatc
     7741 aatccgccag tcagttcagt catcgttcca gctaaatcag tataaatgat tttatcttca
     7801 tctgatttgc ctttattgta atcatctaga taatccaaga tcttttcttt gtcttcaaaa
     7861 ttgttcggat aaatcatgat acttgaaggc aagctgtctc cacctaaata actaagcaat
     7921 gtttgtttgg cattttcgtc aactttttca gtagttaaga catttacacc actgtctctt
     7981 tgagctttga cgatttctga ttctttgttt tcgttgacga tttgagtcgt taattgatcg
     8041 ctataagcga ttcctggaga taagagattc attgtagaag aagatttcac gcgtaggatc
     8101 cccgagatct tcaattcatc agaagcattc tgatacatcg catcataatc tgtattaggg
     8161 ataaaattcc cagttggaag ttttgtatag taagtattgt tgtttactaa tttgaatgtt
     8221 gtaccaacga tatcatcaaa atcaagtttt tcgtcttctt tcacatcaaa tccaagattc
     8281 ttcaatgcgt tgatattagt attattattg ccgtctacga tcagcacgac atctgttgct
     8341 gaggctggat aacttccggc aagtaaagaa taattgtctt ttaaaaagtt ttctttgcta
     8401 gtgtcaagct gtgtagggaa acttgacact cccacacctg tcattgctga catcgtacta
     8461 gataaagaaa gagattccgt atcaggattt tgattcgaga agctgacagg ctgtactttg
     8521 ccatttacat cacgtaaaag attgattcct gtagtacggg taaatccgat attattactt
     8581 aagttaggat cgatgtctgt tacgtaatcg acgtatgttt gatcaatctt attcgtgtgc
     8641 tgtgctttat cttcttcgct gacttttgca gtaatcgttt tgctgtctgg ataatccgct
     8701 ttgcttgcac ccaatccggc atcgtcacga gtctggttag tcgttacctt agaaatagta
     8761 atcggaaatt tcgccatcgt ttcagcttgc gtgttatcga tttgtttttg gaatccagaa
     8821 gaaagggaca gaacgatcgc gattccgatg atcccaatac tggaagcaaa ggaggtcaag
     8881 aaagtacgtc ctttttttgt tcggatattg ttgaaggaaa gctttaacgc tgtccaaaaa
     8941 ctcatctttg ttctgcgtaa gttaaattga tcatcttttg gtcgttcgat gtgaggatgg
     9001 gaatctgtta agatcttacc atcagaaaat tcaatgattc gatctgcgta ttgatgggcc
     9061 agttcaggat tatgtgtgac catgatcact aatttttctt tcgataactc ttggatcaat
     9121 tccatgatct ggatacttgt ttctgtatct aacgctccgg ttggttcatc gcacaaaagg
     9181 atatctgggt cattggctag tgctcgagcg atggccactc gctgcatttg tccaccagat
     9241 aactgatttg gctttttatg catatgattt gtcaagccga ctcggtgcaa tgcatcttct
     9301 gcccgttttc gtttttcgtc tttagaaaca ccacttaatg tcatcccaag ttcaacgttt
     9361 tcaataatgc ctagatgacc aatcaagttg taactttgaa aaatgaaacc gatagagttg
     9421 tttcggtagg catcccagtc actgtcctta aagtctttag tagactttcc gttgattacc
     9481 atatcaccgg aatcataatt atctagtcca ccaatcacat tcagcatagt cgttttacca
     9541 gaaccactag gacctaaaat cgctacgaat tctttttgtc tgaaagcgac agacacacca
     9601 tccaatgctt tggtggtggt ttcgccgact ttataatatt tttttatatc ttttaattga
     9661 agcataatca cccttctccc gtattgaaat attcggtata ctaataagta tatcagaaca
     9721 atgatcgtag tatacgaaat aaatgttaac tgaagatgaa ctacaagaaa aaatctagaa
     9781 atttttcaaa aaacagaggg agatcaatga atcgtatttt agtagtagaa gatgatccgc
     9841 aattacagtt gctttaccgt tctgtgttgg aacgagcggg cttttcagtc atcaccgcat
     9901 tgaatggaga ggatgcgctg aaaaaactag aagcgacacg tatagaatta atcattacag
     9961 atatcatgat gccgaaaatg gatggctatg acttgctaga ctcattacgt agcaccagat
    10021 ttgacacacc tgttctaatg attacggcaa aggccgattt tgaggataag aaacgcgggt
    10081 tcaaattagg agcagatgat tatatgacaa aacccatcga tgtcaatgag atgatcctac
    10141 gtgtggaagc attgcttcga cgttcaaaga tcaattacag cagtagcttg ataatcggac
    10201 aaacaaaatt agagctagat gcttatacag tgttacaaga tcagcattcg ttgattctgc
    10261 ctcaaaaaga atttctgctt ctgtataaat tactgtctta tcccaacaag atcttcactc
    10321 gccaacagtt gatggatgat atttggggat tagatacgaa tacagaagaa cgaacgatcg
    10381 atgtacatat caaacgttta aggacccgtt ttgaaaagac ggaagatttt caaatcatca
    10441 cagtacgggg actaggctat aaggcggtta tttaaagatg aaaaaaaagc tattttcaca
    10501 attatggatc tattttgcta gtattgtctt catttcgatc ttagtgacac tgctgtgttt
    10561 tctcggtttt attttcttat tgtctcgaca ttctatctcg cctgctgaat cggctggaaa
    10621 accaacgtta tacccgttat tcgtctttgc ggggttcagt atggtcgtgg gtacaggaat
    10681 atcgattttt gttgggcggc gtatcctgca tccaatcagt gcgctaggga caaatatgag
    10741 tctggtagca acgggagatt tttcgattcg aatggatgaa cagcagaaag tagcagaagt
    10801 acagcaactg tataaagatt tcaacgttat ggtccaagaa ctcaatagta tcgagacact
    10861 gaggaatgac tttgtgtcca gtgtatccca tgaattcaaa acaccactag caacaatcca
    10921 aggatatgtt caattgttac aagccccaaa tctctctgac gaagaacgcc aaatattttt
    10981 atatcggatt atcgaaagta tcacgcagct ttcacaatta acagagaaca tcttaaaatt
    11041 gaacaaactg gaaaaccagc gtatccaact ggaaaagaaa gagtatcgat tagatgaaca
    11101 aatcagagaa gtaatcgttt tttttcagcc taaatgggag aaagaacagc tagaattgga
    11161 tatcgaactt gctgcagtaa attacacagg caatgaagag tttttgtacc aagtgtggct
    11221 gaatatcatg gataacgcca taaagtacaa tcaaatcaat ggacagattc atatcaaact
    11281 atttgagaca gccacagaga ttgtactgga agtgaccgat tcaggtgtag gaatgaatga
    11341 agagaccaga gatcgaatgt ttgaaaaatt ttatcaagga gatacaagtc gtcaaatttc
    11401 tgggaatggg ctaggacttt cactggttaa aaaaattcta gaactgcatg atggacgtat
    11461 tgattatagc agtatcgaag gagtaggaac gacagcaatg atacggttaa gtaaacaata
    11521 ggaaaaagaa taaaacccct aaaaattcta ttgaaggttt ttaggggttt ttagtcgttt
    11581 ttttgtgatt agggaaatgt tcttacggtt cttttggcat aaaaatcgct gctttcttat
    11641 tgaactacga gaaatgttag aggtgtcatt gaaacaagga catgatgcaa gaaagagagc
    11701 gaaagtcatg cgtcgctctc tttttttggg tggatagatg gacagaaaat gtagggctta
    11761 gttttcttct tgatttaaat actcccaaaa tgtatctgaa gccaaagaat gctcaccgac
    11821 tttacgagta aatgcaagat aactctctgt tgttggagaa atagggagaa tcacagtatt
    11881 ttcttttaat agattcttga ctgttttttc cataacaata gcaatcccca tgtcatttgc
    11941 aatcatattg atgatcaagt cgattcttgc tcctttataa gagatttttg gttcaaatcc
    12001 agcttcgcga cacagatcta gtacaggttg atacaagttc gtagtggggc caaggagcaa
    12061 aaagttctcc tttttcagtt cgcttagatc caatttcttt ttactagcta aaggatgatt
    12121 tttaggtaga actgctacaa aatcgtctgt ttcagtaggc aaccactcaa tcgaacttgg
    12181 tggggcctgg aaacttctag caaagtggat cgtgtcacta tgttcattgg cgtcattgta
    12241 agttaattcg acactttcca cttctttcaa ctggactgta aattcaggat ggcgtttcaa
    12301 aaactcggtg attttggaaa aacctttata attgatcatt gtcggtatcg ttaagatatt
    12361 taatgaaaga ttgttgtctt tcgtataggt ttcaagcact tcttgcatag catggtattg
    12421 ttctacgaca ttttttacat atgggagcaa taagcttcca gcttcggtca attcgatttt
    12481 tctatgggcg cgttcgaata atttgacgtc taattctgtt tctaacgaaa gaatctgttt
    12541 agaaatattt ccttgggtgg tgaattgtcg atcagcagtt ttggtgaaat tcattgtttc
    12601 ggctaaatca agaaaaactt cgaatttatg gatatccatc atattcctcc tacttattcc
    12661 aataaagaat actttaacac aaaaaatgaa ttggtgtaat cttctctgtc tgttacaatc
    12721 tttcttgcag cgaaagcaat aattacagaa atttttctga tttttttcgc ttcgtagaaa
    12781 aagagaggag ttttttgatg ccaactggag ttttgatcaa tgtaggttcc gttttgtttg
    12841 gcggactaat tggcggatta gtaggaaata aattaagtga acattttaaa gcacagctga
    12901 cgatggtttt tggtgtgtgt tcgatgggga tgggaatcta ttccattgcg ccgatgaaaa
    12961 atatgccggc tgttattttt gctttagtga taggaacagc gattggtttg attgttcatt
    13021 taggtaatgg gataaataag ggggctgcgc tgatgcaagt acctatttct aaaatcttcc
    13081 ctagtgaaaa gcttggtatg acccacgatg aatttatcag cacattagtg acggtcattg
    13141 ttctattttg tgcaagtggt acggggatct atggaagttt agattctggg atgacaggag
    13201 acagtacgat tttgatttct aaatctgtcc ttgatttttt cacagcagct atttttgcgt
    13261 gtaatttagg gtatgtcgtt tctgtgatag caattcccca attcattatt ttttatattt
    13321 tgttcttatt ggcaaaattt atttatccat tgactacacc agacatgatc ttagatttta
    13381 aagcttgcgg cggattcttg atggtcgcta ccggttttag aatgatcaac gtgaaaatgt
    13441 tcccagtagc tgacatgata cctgcaatga tcgttatcat gccactgagc tggatgtgga
    13501 caaattggat tatgccactg ttataataaa aatcaaagag aagccatttt ttgctgacta
    13561 aaaaggatca aatcttttag tcagtttttt tgtatttgac aaactgattt tgaaactata
    13621 ttatatgttg tataatatag attgaaataa aatattacac ttgagaatag aaggtgacgt
    13681 aatgcacatc cagattccaa ctgttcttct tgatggtact gtgctagcag cattaagcaa
    13741 ggaagatatg tatgggtatg ccttaacaaa aatcgtacaa gaaagtctct ctgtcagtga
    13801 atctacacta tatccggttt tgagaagatt gaaaaaagaa ggatatcttg agacctatga
    13861 tgaacctttt gaaggcagaa accgtcgata ttactcgttg acaccaatgg gaagtcgtca
    13921 tttagaagaa attattgatg aatggattgt ttttcgagat tctttagacc ggttattgga
    13981 ggagagcaca aatgaatgaa tatttgaatg aagttgtgga tcatcttcaa gaaattcctg
    14041 aagacgatcg ttttgatttg atccagtatt atgaggaata ttttttagat tctggaaaaa
    14101 cattagatca gatcattgat gaatatggta caccaaagca gtttgcctta aaattaaaaa
    14161 tcagttactt ttcagaagaa gaacaagcag aaaacgagtt agtgaaaaat ccaaaaagac
    14221 aaattcgcct gatttggtta atcattatcg gtttatttgc ttctcctgtc ctcattccac
    14281 tagcactggg gtttatattg gttattgggg gactattggt tgctttacta gcgctcattt
    14341 ttagtatcta tgttttatgt atcagtatct taggtgttgg cctattcatg atcatctcag
    14401 gaatcagtgt gtttggacaa tcgattctga gtggattatt ctttatcggt gtcggtctgt
    14461 tcgccacagg aagcgcagta tttatttcgc cactgttgct gaaaggaaca aaagggcttt
    14521 tttatgtcat gatgaacttt gttaaatgga tgggacgacg ttttattaca aaaagagatc
    14581 tgcattcagc gggcgtttga aggaggacac ataaattgag attgaaacat tttttgatta
    14641 ccggtattgg tctgatgctt actggtggcc tcctagcagg tgcaacttat acattaggcg
    14701 ctcaaaagag tctgacttgg gataatggtc ctcggttgat cgatatggtt actgagacaa
    14761 aaaaaatcaa ggatgcaaac aaaatcgtca tccatgcaga aaatcaaaca gtttcggtca
    14821 tacgcggatt ggattttgag ataaaaacga cttatgataa gacagaaaag ccagaaataa
    14881 aggagaaaaa tggcgtgttg actgtgaatg cagtcaaaaa atcaacaggt gcaatcatta
    14941 gtttagaaga cacaagtgca caaatcacta ttgttgtccc tgcagatcat gaattatctg
    15001 aattgacaat cgaagggaat aatagtgcaa ttgatatcaa cgaagtatca atcaaaaaag
    15061 tgaatactaa gacacatgat ggctgggtat cgttcaatga atttacagga ggaacaataa
    15121 ctgcgaaaga tgaattaggc tcacttcgat tagatgaggc caatgtggat aacttgatcg
    15181 tcacaggtga aaacagcaac ttccatctag aagacagttt gatcagcaaa gaaggaaaag
    15241 tcatcctaaa cggtggcaat ttatctttgc atgagatgga aaacagtggc tatactatcc
    15301 aaacgtctgg tgaatcttat attgaaaaag actaccagcc aataacaaat actttgttag
    15361 aacaaggaca gaaaaagcta aagatcactg ctaaagacac atcgatcaat attacgacgg
    15421 atcaagagga caatgaaaca gattgatcct gtactcaact ggatatttta agaaaaaaca
    15481 gcaaagatcc ttccaatctt tgctgttttt catcgcttaa cgcacataac tatagtaaaa
    15541 taagaaagaa aatatctaaa aagaaaacag ggtgaaagag tatatgaagg tagaagaagg
    15601 tcaaagagaa aagttaaaaa cagaaatgga aagattacat acttatatca ctcaactaag
    15661 ccaaaccttc tatgatccag acaaagaaaa agtgatggtt aattacccga acaattcaga
    15721 aggtcgccaa ttagagcaag tgtaccatga agttttcaaa catttattga cggtgaagaa
    15781 agagctggat tattattctt taccgattat cgatacagga atattgaagt atgatgggaa
    15841 gaaggaacga tttatcttta aatctgttcg ggaagatttg cctctttcag cagggatgga
    15901 tctagaggtc ttggtagaag actattttac agaagaaaag cactgggttc gaacaaaatt
    15961 ggattattta ccgcaggcag ccggagggac acaggcaagc ggttggtaca tcactgaaga
    16021 caaagagtta gaactagaag gtgtaatggc gcgtattcga aaaaaaaatt aaaaaagtaa
    16081 cattagatat ttattgaagg ataaaggagc gcaggaagta gcatgagaga aaaaagagca
    16141 ttttatatca atggttatgc agggattgtc ggattggtcg tattagcttt agttggattg
    16201 tttttcttct atttaggtat gtggcaagcg aaagtttggg cacttttttt aagtattttt
    16261 ctttggttga tcacgttatt gttgttaagt tcggcaactg tagtcagtcc taatcaagca
    16321 aaagtaattt tgttttttgg acaatattta ggtacgattc gagaaaatgg ctttttcctt
    16381 acgatacctt tggcacaaaa aatgactgtt tcgttgaaag ttcggaattt caatagttct
    16441 gttttaaaag tcaatgattt ggatggaaac cccatcgaaa tctcagcagt agtagtcttt
    16501 aaagtcgttg atacagcaaa ggcattattc gatgtagcgt attatcaaga ttttgtagaa
    16561 atccaaagtg agacagccat tcggcatatc gctagccaat acccgtatga tacattcaat
    16621 gagaatgatt tgacgcttcg aggaaacacg acggcagtgt ctgacgagtt gcaaaaagag
    16681 ctgcaagagc gtttagcagt agcaggtgtc gaagtaatcg aaacacgctt gaaccattta
    16741 gcctatgcaa cagaaatcgc tagtgccatg ctccaacgtc aacaagcaaa agccattttg
    16801 tctgctcgac aaaccattgt tgaaggagct gtcacaatca ctcaaatggc cttagagcaa
    16861 atcgaagatg gacaagaaat caatttcacc gatgatcgga aagtccaatt gatcaataat
    16921 ttacttgtct caatcattac agacaaagga acgcaacctg ttatcaatac aggcgacatg
    16981 aaagaccgta agtgacatac tttttgtaag ataggaagaa ggagtttagg acggattatt
    17041 gtcttgaact ctttttttag ttgttttaaa gaaaatgaca aaattgaaag aagaatgata
    17101 gttgattcga aggcagaatt gttcgctttt ttctataatc agtatataaa gtaagagaga
    17161 aggggaaaaa gtgaaaaaac ttctgatcgg tctacttgtt ttcatcgttg tggtacttgc
    17221 aggggtgaaa atcgcagatt acgtagttat gggtggagaa gaatactatg tacaaattac
    17281 gacagatggt acaaaaacag tttcaaaatc agactctgga caagaaacag tcaactatag
    17341 atatatcctg cctggctata cgaaagaggg taaggaaaaa gaactggact tcaagggaca
    17401 attgactcga ccgcttcgta aaggagcata tttaaaaatc acctggaaca aaaataaagg
    17461 tgtcacttcg tatgaagaag tcgaaaaaaa agacgtgccg aaaacagcac tggaaaaact
    17521 aattaaaggg tagggaaaga aatgaacaaa atagtcgaag tacagaatgt aacaaaagta
    17581 tacggaaaaa acaatgagaa aaagacgcaa gcattaagcg gcatcagctt cgatgtagaa
    17641 aaaggcgaat ttattgggat catgggcgct tctggttctg gtaagtcgac acttttgaat
    17701 attttatcca cattagataa accaacagat gggcatatcc gaatcaatca aaaagatgtg
    17761 accacattaa aaggaaatca actagcagat tttcgtgcga atgagattgg ctttattttc
    17821 caagatttca atttattaga aagcttgaca gcacaagaaa atatcgctgt tcctctttct
    17881 ttacaaggcg tcagaccaaa agaaatcaag caacgtgtcc aaaaaattgc agaacgttta
    17941 tccatctctc atattttgga aagctatcct tctgagattt ccggtggaca aaaacaacgg
    18001 gtcgccgccg cacgtgcatt gatcacacaa ccaacgattc tattagggga tgagcctaca
    18061 ggtgcgctgg attccaaaag tgcccgggat cttttagata cgatggacga attgaataca
    18121 aaagatcatg tatcgattct tcttgtgacg catgatccgt tttctgcaag ttattgccaa
    18181 cgtattttat tcatcaagga tgtcggtatc catcaagaag tgaaacgtgg agatcaaagc
    18241 cgtgattcct tctatcgaga aatactatct attcttggaa acttggaaca gtagggggag
    18301 attgactatg ctttggaaat tatcacttac tgggatcaag agccgtttaa gagactactt
    18361 ggtgttattc tcaggattag ttatggcatc tgctattttt tacatgtttg aatcaatggc
    18421 aagcaacgaa gcttttttga aaagtaatac gatcgttgcg gctacagtta tcattttccg
    18481 attcggttct gttttactag ggatcatcac attcgtttat attttatatg caaattcctt
    18541 tttgatgacg atgcggcaaa aagactatgc aatgtttatg atgctgggag caaaaggcag
    18601 aaaaatcgca cagatgattt tttctgaaac attcttagta gggatcgcag ctactttagt
    18661 gggttcagct gtcggtgtag gattgacaag tatcgtccac cgattattag tggatcagtt
    18721 agatctcaat atttcgcatt ttacaccttt tagtattcac gggttgttga tcactgtttt
    18781 gttttttgct gttttgtttt tattagctgc tgttgtcaat gctttttcga ttgtcaaaaa
    18841 accgatttta gcattgattc gtgcggatca gacaccaaca agaatcaagc agcataaatt
    18901 cttgtttttc cttgaagtag tactgggaat tgcttttcta ggctgcggtt actatatgat
    18961 gaaacacgtg gggacctatc aagtctttgg tataggggta gccttagtta cgatcgtatt
    19021 aggaacttat ttcattttcc attctgtgat cattttcttt ttatcattac taaagaaatc
    19081 agaacagatt tcattgaaaa aattgaacaa tttcacattg tcacagttaa actttcgtat
    19141 tcgtgaatat acacaaatgc tctcaatggt cgctatgctg tttgccttgg cactgggcgc
    19201 tttgacggta ggattaggtt ttaaaaatga aattgcagac ttgacagaaa acaattctgg
    19261 atatgatcta gtgatgaata atcctcgggc agaagatcag caaaaaatca aagaactttc
    19321 accaacactt tctgcttctt atactcaaaa agaagatgcg ggaacgattt actataatgc
    19381 aagtgaattt gcggctcaac cattgatcat gataaaacat gaagatggaa caccgccgaa
    19441 gatcacgaaa gaaaaagttc ctgctaaaaa aatgaatgaa ctgactgtac aagaggaact
    19501 tcgaagctat gaattgccgg aacagcaaga aaaagaaatc aaggtagtca gtcaatcaga
    19561 atttgatcaa ctgaacttgc cacaatccac tttacagcta gttcaggtaa aagacttcca
    19621 agcaaacctg aaaccaatcg aaacacttgt tgctcaaaat aaaatgaaca atccatcttt
    19681 gcaaaaagta gagtacagca accagaaata tgaaatgtat gaagtgtata atggtctatt
    19741 ctctggtttt gaatttatgg gattcttcct aggtttagct ttcttgacca tgcttgctag
    19801 ctgtttgatg ttcaagatct tgtcaggaag caagagtgac attgctcgtt atgaaatgct
    19861 tgaaaagata ggaacacgac gcgggttgct taaacaatcg atccgtagag aagtaggcgt
    19921 gttattctta gcaccaggaa tcttaggtgt agtccatgtc ttattcggat tacaaatgtt
    19981 tggtctattc atgcaaaatc catacaaaga tatttgggtt ccgtttacga tattcttcgt
    20041 attgtatttc atctactatg tactgacgac atggttatat actggaatcg tattgaaaaa
    20101 gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagctcc
    20161 tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttgt
    20221 tcggaaaaag aggttgtgac aatatttttg tcacaacctt tttttcacgg tcttttatta
    20281 gaatatagta aattttttct cgttactcac ggaataagct gttaagaaaa cggtttccat
    20341 gtatactgga atcagtgcaa atgaaagaaa agagggtgca aggaacatgg aaatcaatga
    20401 acaaaatttt ggcgaaaact atcacttgat tacgatcacg aatcaaaatg ggacatcttt
    20461 gtctgtcagt gatcttgggg cacgggttgt tcattttcaa acaataatcg ataatgaaaa
    20521 acgagagttg atcttaggtt ttgatgaagc aaaagaatat ctagaaaaag acccttatat
    20581 cggtgcttca atcggacgta tggctggacg gattacgaat ggcacattca aattagatga
    20641 tgaaacgttc gaagtaaaaa cagacccgga cactggtcat tgtttacatg gtggtgcacc
    20701 tagctttgaa gccaagcagt ggcattatac agtcatcaat ggtgaaaacg aagcatcggt
    20761 cattttctat cagactagcc cagatatgga aaacggattt cctggaactt tagatgtaga
    20821 agtacggtat acgctgacga acgatgatac atggcgagtg acggtccaag ggatcagtga
    20881 ccgcaaaaca gtattcaatc ctacaaatca tgtctatttc aatttaaccg gtgatccagc
    20941 acagtcaatc gacgaacata tactttggtt gaatagccga ttatttgcta cgctcaataa
    21001 agatacaacg cctacaggag aagtagtttc agtaagtggc acagcatttg acttccaacg
    21061 tcctaaaaaa ttaggtgagg tctttgccag tgattttcgc caatcaatga tagtgggcgg
    21121 catcgatcat ccatttttgt tgaaaaaaca atctggttta ctaaatgctg cggaattgac
    21181 aagcccagat gaaaaagtat cgatccaagt aaaaacagat gcttcaggaa tcgtgatttt
    21241 tacagcaaat tttggagaag atggaccgga aatgcgcggg aataaattag taaatcatgg
    21301 cggtatcaca tttgagactc aagaactgcc aggagcggag cgacttgaaa gttttggaga
    21361 tgtcactctg gaacctaacc aagtacgcga gtttgtaacg gaatacaaaa taaatataaa
    21421 gaaatagaat aaattaaaaa gggagtgtga cagtgtcaca tccccttttt aatttattct
    21481 atttctttaa aggcagatca agccagaatg atttgtctaa acttaacaag gttagtacag
    21541 gaacagttgc agctttaact gtacgagtag ttgtttctgg tatctgtctt gccgtaaaat
    21601 tgttccacaa atcccagatt gaagcgacac ggaagatgga agaagcgttg ttgacgcgtt
    21661 ttatcgttga aaattctaat cctgtgattg gcacgccatc tgttgtccat ttgattggca
    21721 catcgaatcg cttagacata ttgaggattt cagcgtttgc ttcttcagtt gttgctgctt
    21781 cttttttcgg ttcatcatat tttgtcaggt cgtacgcttc gatgatcgcg ttcaattttt
    21841 gtgaatattg gcgatctgta gcataacgtc cttccaaata tttggttgct tctttgtagt
    21901 cttttgtttc cgacttccaa gttggtttgt agaaatcagg attgccagtc agtccatttt
    21961 tgatcaagtc ggcatagtct tccaaacttt ccttgtaaga aggatattta cggaattgtg
    22021 cgcggatttg gtagctttgt ccggaactat cttgttcaag tgtattgaag gtcacgctgc
    22081 ttcctttata tgatcctttg ataccaaaaa ggttatgatt tggttcgctt gataataagc
    22141 tgtttccaga tccactttca aggatagctt gagccatcat gactgatgcg tagattcctt
    22201 ctttttgccc gatttcctgt gcatcatctg caatcttttt caagaactga tcagttgttt
    22261 ggttttgact gaaatggaac gagccatcta atccatctaa aatagatgag ccgcctgtca
    22321 gcgaacctga agcagcaggt aaacctgcta agtttactcg gacagcaaaa tcaggcgtga
    22381 aatctgaaat agaagttact cgaactttat ctcctggttg aggcgcatgg ataaattgtc
    22441 cgttcccaat atagatacct acatggtgag taacaccgga agaaggtccc cagaagacga
    22501 ggtcgccagg ttgggcctga cttactggaa tctgcgtacc tgcatattgc tgttcgcctg
    22561 tccagccacc gatataatga ccggttgctt tcatatatac gtaataagtc aaaccagaac
    22621 agtcaaaagt atttggacct tttgctcccc atacatatgg ttttccaaga tgcttgaatg
    22681 cttctcgaac gattgcttgt tggacattcg ttgtatttgt aggcaataca gcatcaattg
    22741 gtgattttac aacaaaatca gaagaagcag aagatggatt ctctataccg ctttgatttg
    22801 ctgtatttgg acgagatgaa gcattgttcg ttccgccatt cgatgaacca ttgtttggat
    22861 ggtttggttc tctctgagta tttccattgt tattcgatcc agatgggttt gtttgattcg
    22921 aagaattccc gctgttcgaa ttattgttat tattgtttga attatttgaa ccattattat
    22981 tgttgttatt acccacatct ggtttgtttg gcgtttccgg atctggtgta ctagggactt
    23041 cttcactagg atcagttggt ttctctgttg aagtatccgg tccagcttta aattctgcag
    23101 taatagataa attgttcgta cctactgtaa aggtctttgc atcataattt acttctttgg
    23161 caccattaac agcgactgaa gcaatttgtt caccttcagc tggcgtaata gtgtaagtta
    23221 ccttcgtttc cgctaataaa ccttctaccg ttccactttc cgtaatatcc aaaggttgct
    23281 gttcagggga atccgctgtc gaagcagtca gttggatctt accaatcaaa gaaggagcaa
    23341 ttgtaaggga atatgtctcg gctggctttt cattttctga tggatcagcc ggatcaattt
    23401 cagttggctc ggtcgatcct gttgttgagt tagttgttcc agttgggtct tctgtaaaag
    23461 aatccgttgt ttcactagta gctgagctgg atgtagtcgt atctgttgtg actgtttgat
    23521 cagctatttg attatctacg gcaaatacgg tattggtcga caaaacagac ggagcaatca
    23581 gccaagtagc agctagcaaa cgtatcgttt ttttattttt cataataaac tagtcctttt
    23641 cctttcattg attttctgtc ttattcagca ttcattagac attttttgga acagataatc
    23701 gtgtcgaaaa gctttttctt ttcataaatc tgtctgtgta ttctaaataa aacaatttgt
    23761 aaataagcaa cgagcattca ttcgtttttc tttgacgttc attttattgc atagaccacc
    23821 tttgtttggg gataaacaag tggggataaa aatggttatg catatgtaaa ttataccaaa
    23881 gtttggaaag tatcacaaaa atttataaaa ataatgatcc taaaaagaaa aagactgaaa
    23941 tatgtgataa acatctcata cttcagtctt tttcattaac tattcataag acattaggtc
    24001 ttcatcaaac ccgctaacga gagtagtgat cattgctacg actagactaa cagccagtcc
    24061 tgctaagtac cagaaaaaag taccggaaat gaatgcaggc aatgtggtca gactaccaaa
    24121 tacaaaagcg atcgctttca cttgggcaaa tcccattaca gctccaccag cagctgcacc
    24181 aatgcaagca gcaacaaatg gttttttcaa tgggatcgca attccgtaaa cagttggttc
    24241 ggtcactccc gcaagtaaaa tcgaagcaaa agcagaaccg gagatcgaac gggtttttga
    24301 acgtttactt cgcagaaata cacctaaagc tgcaccagcc attccaaaat tggctgctcc
    24361 tgtcaacgga acgattcggt cgaacccata agcagagata ttactgatca tgacagggtt
    24421 tacagcccat tggataccaa atattaccaa aatattccaa ccaccagcga tgattgcgcc
    24481 actgatcggt ccgttaatat ccatcatcca agtaattgcg gcagctaaac cttcgccaag
    24541 gtaaacaccc gcaggcccga ataccattaa cgtcaaagga accgtgataa ctaatgaaag
    24601 taaaggtaca aatactaatt gcatattttc gcgaatatat tttttgagga atttttcaac
    24661 aaaagaataa aagaaaatac ctaaaatcgc cggcatgaca gtagacgtat actgcatcag
    24721 tactacagga atccccagaa aatcagtagc tgctttgcca ctgtctgaaa gtaaagaggt
    24781 cagattcggt tcaatcagtg ctgcagcaat gacagccgca atatatccat tcactttaaa
    24841 cgttttggca gcagaaaaag caagaaagat tggcagaaaa taaaagacgg cattcgacgc
    24901 agcagacaaa atatgataag ttccgcttgt ttcactaagc cagccaatcg tagtcgctaa
    24961 aacaaccaga ccttttaata taccagaacc ggcaaacagc ggaatgatcg gcgtaaagat
    25021 ttccgatatc aaaccaaata gtttttcgat cggatttagc ttttctttcg gagctgaatg
    25081 atcgatttga cgcgtcaatt ccttgtaagc aggagccaat ccctgatcca tgattatttc
    25141 tgcatatcta tcgagtaaaa tgaaatactt aatggaaggg atttgctgtt ttaatttttc
    25201 tgcattggct actggctgtt ttttcaattt gatacgaagc cgagtcgcac atggaaggac
    25261 agaatcaata ttgtcttttc ctcccaactg ttcaatcagc atatgaatct gctcggacag
    25321 taccatctgt tcaggattca ttgtcgtttc cattagaaga ccactccact cactaatata
    25381 atattggcgt aaggtgtcat ttcgcctgta cgaataaatg catgacagtt attcaactct
    25441 tttttcattt cttcatgtgg gataaagcta atcggtgtat ctggtaacaa taatttgatt
    25501 ttttccaata tttccgggtt cattgtcttt atttcttccg ccaattcaat cgattgtacc
    25561 tcaagttctt ctaaaacgtt ttctaaaaca gataaaaaac ttggaagacc gtttgagaca
    25621 gccaaatcta ttttttcagt accaaagggg acaggcatgc ctgcatcccc gatacttaat
    25681 ttatcaaagt gccccatttg tgcgattact cgtgaaatat ctgaattgat tacttttcct
    25741 tttttcatgt ttgtttcttc ctctcattgt taattttaga ctgctgattg ttctaaagct
    25801 tcgcggtaag ggatagaagg ttgcgcacca taagactgca cagcaacaga agaggctaga
    25861 tttccataaa ggatcgcttc tttcaagtta ctaaggtctg gctgaagtgt gctgcttaat
    25921 gcgccaataa atgtatcacc tgcggctgtt gtgtcaacgg catccacttt aaaagcaggg
    25981 acgataccgt gttctttttc tgtatgatag aaagcgccgg cactgcctag tgtaatgatt
    26041 actgttccaa tacctaattc atggagtttt tcggctgctg cgaccaaact gttatgatct
    26101 gtcactctga ttcctgtaat gatttctgtt tctgtttcat ttggtataat gatatctgtc
    26161 ttttcaagta gttcagtagg aatcgtttca cgagctggtg caggattcag gatcgttgtt
    26221 tttcctgcgt cttttgcaat agaaaaagct aggatcgtcg catctaagct actttcaaac
    26281 tgagcaataa taaagtcact gtttgtaatc agttgcttgt tttttaggac ttcttgttca
    26341 tggaaagcat tatttgcccc tgcatggatc aagatactat tttctcctgc tgcgtctacc
    26401 ataatcattg cttgtccggt tgtggcgcct tggatttcag ctatcccaga tgtatcgatg
    26461 ttttcttttg tcagtagatc taataactgt tgtccttcgc tgtcagcccc aacgcctcca
    26521 ataaaagatg tccgtgcgcc tgaacgtttg gctgccactg cttggttggc acctttacct
    26581 ccgccagcgt gaaaaatctc atgcgcatgc atcgtttcgc ctggcttggg catgttggtt
    26641 aaacgaaggg tagtgtccat attaatactt ccaattactg taaccgtgtt cattttacaa
    26701 acctccaact atctcattta ttttgatggg aaaattattt tttaaaacgt tttagtaaaa
    26761 cgttttaata gttcgataaa aatatagcac accttgaaag ctatttcaag ggtgcagttg
    26821 aaaatctttt ttctaatcgt acaggtaatc ttttttcttc ccattctttt tctgggaatt
    26881 ggatccgatc taacaataac ttggcagatg tttgtcccaa ttcgaagatt ggttgtgcga
    26941 tcgttgtcag tttcggcttg atatactcgc acatatctat attatcatag ccaataatgc
    27001 tgtagtcttc agggatgctt tttccagctt cctctaatcc gcgatataat ccaaaagcta
    27061 attcatcatt taaagcaaac acaccagttg cagcagaatc aagaagttct gctgtgatct
    27121 ggtagccacc ttgttttgag aactgtgtag gtaagaggat cagttggtcg tgagggattt
    27181 ggtaaacatc aagagcagat ttaaacccct caatccgagc gtggacattt tccggtggat
    27241 tttcaggata gacaagggcg atcgtttgat gtcctagact aagcaagtgc atgcccgcta
    27301 ggtaaccacc gcgaaaatcg tctgttcgta cggcatcact aaatccttca gattttttct
    27361 gatccaaaac aatgaagggc cggccttgtt ttttcaaatt ttcgttgatt gcatctgtag
    27421 agacagcaga agtcgcgata atgaatccat ccacacctcg acgtgtgagc tcggctagat
    27481 attcgatttc tttttgatga tcagaatcag cattacataa tatcgtgacg aaattctgct
    27541 tatacaagat atcctcaatt cctcgcatca gtgtactgaa gaatggattc gtgatatcag
    27601 gaacgagtac accaattgtt ttcgtttcct tggtaatcat ttgtctagca aaatagtcag
    27661 gctgatagcc taattcttct ttggcagcta aaactttctg aacagttttc ggactgaatt
    27721 tctccgtatt tccatttaat atcaaggaga cagttgtgat tgaaacgccg gaaagtttag
    27781 cgacttcttt gattgtgatc ttttttttca ccatgttcat actcctatct aaatcctgtg
    27841 gttattttcc catgaaatca aaaagaagta aatgaaaatc acgtgtgcca gtacttattt
    27901 gctttgttgg atagcttcga ttatttgttt cgttgagcgt actcgtgcag ttttcggaaa
    27961 tatctttttt atgaacaatt gatgggattc tttgtcacga tcactgcaag catcttcagc
    28021 aagcacgata tggtacccgt gttgaaaggc atctaatgca gtagcatata caccgtttgt
    28081 tgtcgctaca ccgcttaaaa tgatagtttc gattccacgc cggcgaagtt gaagatcaag
    28141 agaagtacca aaaaaagcac ttggattgta ttttgtgata gtcaacatat tatctgtatc
    28201 tttcaaagaa tccttcaaaa gtaaatcagt gaattctgga ggtacagaag cagtcattcc
    28261 acccatatca gtttgttgac tcaaataagg gaaagtggag atgtctacgt tcactaaaac
    28321 tttcaaggca gcggtacgtt tcaatgttct tactaacaaa tcattgtttg tcagaataga
    28381 tgctgcagtg tgtggagaga gtgtaccgtt atttacgatc tctttttgca tgtcgatcgc
    28441 gacaaaagca gtttttgata aatcaaaaat catattttcc tttaaatcat tcatgatgtt
    28501 ccttctttca aataacaatg ttttttatgt aagtaaatca taaatcgttt atttgtcaac
    28561 taaattaaag gattcctttc tttagtctag agaaattcag tggtaaaata aataagtgtg
    28621 agaaaacgca ttcgatgcat agatatagcg agaaatatcg tgaaaatgga ggaatcagag
    28681 tgaagtatgt cgaagtaaaa aatgaataca aacgatacaa aatgggagaa acgaccatta
    28741 cagcaaatga cgatatttct ttttcaattg aaaaaggcga attagtgatt atacttggtc
    28801 caagtggagc aggaaagtca acgattctta atattttagg tgggatggat acacctgatg
    28861 aaggtcagat cattattgat gacacagata ttgcgcaatt ttcagataaa cagcttactg
    28921 cttaccgacg aacagatgta gggttcgttt tccaattcta caatctcgtt cctaatctaa
    28981 cagcaaaaga aaatgtggaa ttggcaacgg aagtttctcc tgatgcgctt gatcctgtag
    29041 aggtattgag gcaagtgggc ctagctcatc gattgaataa ctttccgtcc caattatcag
    29101 gaggcgaaca gcagagggtt tcaattgctc gtgcactagc taaaaatccc aaacttcttt
    29161 tatgtgatga accgacaggc gcgctggatt ttgaaacggg caagcaagta ttgaaattat
    29221 tgcaaaacgc tagcagacaa cacggcaata ccgttttgat cattacacat aattctgctt
    29281 tagcgcccat cgctgataga gtcattcaca ttaatgatgc taaagtaagg tctgttgaat
    29341 taaatgatca tccttcatcc attgatgaga ttgtatggta gggggatttt atgaaaacaa
    29401 gaacatatct taaagcaggt tttcgcgaaa ttcggcaatc taaaggacga ttcattgcaa
    29461 tcatccttat tgtcatgcta ggaacacttt tatttgtagg tgtcaaaact gctggacctg
    29521 tcatgcaaaa aacgatggat cactatgttg gtacagcagg actttctgat ttgcaaatcg
    29581 tttctactgg tggcttgaca caaaaagata ttgctcaagc agaaaagatc ccagatgcaa
    29641 aagtagaaac aggaaaacag atctattatt ccaatactgg caaaaacgaa gtgattcaaa
    29701 tattttctta taacaaaaat aacaagcaga acaagctaca attaactgat ggaaaactcc
    29761 cggaaaaacc aaaccagctt gtattggatg aaaaagcgga agatgaaggg tataaaatcg
    29821 gtgatattta tcagattgat tcagatgaat tgaaagaaaa agagtatacc atcacgggct
    29881 ttgtccgttc tccattgttc atcaacaatt tggagagagg atacgcgaac gtcggaaatg
    29941 gtagcgtcga ttattttgtc tatttacctg aatccgtttt taaaacagat atttattctg
    30001 tcttatatct tgattttaca aacgtaaaga atttgaatac gtacagcaaa gcttacaaag
    30061 ataaaatgga gcaaaatcaa gaaaaggcag aaaagtattt acaagatcga cccgaagaac
    30121 gcttagaaga gctgaaaaaa tcggccaacg aagaattaga accggcacag caaaaaatag
    30181 cggatggcaa agcacaaaca acgcaagcaa gaacacggtt agaagatgct aaaaaacagt
    30241 tagcgcaaca gtcagcaact atccagcagc ttcctgaagc gcaacgaaca atcgctgttc
    30301 aaacattatc tcaacaagaa gctcaacttg ctgaacaaga aaggcaatta gttgagaaag
    30361 aacgtgaact ggcctctgca gaggaaggat tgaatcgaaa tgcagaaaaa attgaaaatt
    30421 taactaaacc atcataccta tacaatgaac gttcagaaaa tgtcggtttt caagaatttg
    30481 gtgatctgtc tgatcgtatc gctgcgattg ccaatgtttt tcctgttttc tttttcttta
    30541 ttgccgcatt gatcaccttt acgacaatga cacgtatggt agaagaaaac cgcagggaaa
    30601 tcgggacatt aaaggcgtta ggttatacga aagttgaaat cgcaaaaaaa tatgcaatct
    30661 atgcaagtct agcctcaggg ataggtattg ttttaggaac gatattagga acgaatcttt
    30721 tgccaaggat catttttgag ctgtctaacg agcggtatga tattgggagt gcgcttattt
    30781 tttatgactg gccgccgatt ttacaagcag ctgccgcatt cttcattgcc gcatttggcg
    30841 ctgcaatgat cgttttattc aaagatttaa gagaacggcc tgctgcattg cttcaaccga
    30901 aagcaccgaa gccagggaaa cgcatttttc ttgaatacat cactccgctg tggtcacgcc
    30961 ttagctttaa tcaaaaaatc agttaccgaa atctcttccg atataagtca agaatgctta
    31021 tggcgattat cggaattgct ggatgtgccg ggctgatggt tgcaggcgtg ggattgaaag
    31081 attctctaag ttctgtttca gccaaacaat ttggaccgat cattgactat caagcaatcg
    31141 ttacattgga ttcatccacg gaagatagtc gtcaaaaagc agcggatatg ctgcagaagc
    31201 aagaaaaagt aaatgagatc ctagctattc atactcaaac cgtagaattg agaaaaaaag
    31261 gacatgcttc gcaaagtctg acagtgatcg taccaagtaa aacagctgat ttggatcgtt
    31321 ttatccattt aactgatctt gaagagaacc cacgctcctt gcctaaaaca ggaatcgcta
    31381 ttacgcaaaa agtagcagaa ctatttgact tatctacagg agatcatatt tcgctttatg
    31441 atgaagatca aaaagaatgg aaagtcgaag taaaagaaat cgtgaaaaac tatctaggta
    31501 gctttattta tatgtcccct gaatattatg cttcagttac tgatcaatcg atgatggata
    31561 atgcattttt gctgcgtaca gataagatga caacaaaaga agaagaaatg ctttccgaga
    31621 aactgcttgc cacagacgct gtgacaaata cgtctttcat gtccaaacaa atcgaaacgc
    31681 aagaagaatc aatggcgaac ttagactcgg ttgtcttaat ctttgtcgta ttatcaggac
    31741 tgctggcttt tatcgtcttg tataatctaa ctaatatcaa tatttccgag cgtgtacgcg
    31801 aattatctac aataaaagtc cttggatttt ttgataaaga agtcacgatg tacattgttc
    31861 gagaaaatat catctttact cttttaggaa tcatcggagg atttggtgta ggatatatat
    31921 tgactgactt catcttgcaa caagcgtcta tggagaatgt catctttcca ttagtgatca
    31981 catggtgggc ttatgtctta tcagcaggat taactatcgt atttacggtt attgtgatga
    32041 ttgttaccca tttcaaatta aagcatatcg atatgattga tgcgttgaaa tcgaatgaat
    32101 aaaaacagag gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggcaaaa
    32161 tagtttttca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacca
    32221 tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa aaagaatact caaataaaat
    32281 tagctcctca tgttttacat ttgagaagac tgattgccgt agataaaagc ttttgaacac
    32341 catttataga agaaacaaga atattttgct attttaaaca agaggataga caaatacatg
    32401 tctgatctct tgttttttat tgggcaacag acctcttaga atgtataaag ataaatttta
    32461 caaacgcttt cggttcagtt ataataataa atgataactt gtatatataa gttgtggtag
    32521 gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc
    32581 aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa
    32641 tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta
    32701 tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc
    32761 ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga
    32821 tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa
    32881 aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg
    32941 attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt
    33001 atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag
    33061 aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg
    33121 atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca
    33181 aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa
    33241 cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa
    33301 attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc
    33361 atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag
    33421 aattaaagcg actatttcat acatggggag aaaagatgag tgagggtaac ggctggaatg
    33481 ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta gctatcgatc
    33541 agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc
    33601 cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg
    33661 actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag
    33721 aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc
    33781 aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag
    33841 gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct
    33901 attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc
    33961 atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc
    34021 agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat
    34081 tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg
    34141 agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc
    34201 gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt
    34261 cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt
    34321 tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa
    34381 aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa
    34441 tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa
    34501 gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg
    34561 caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca
    34621 acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc
    34681 agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt
    34741 gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg
    34801 ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac
    34861 cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg
    34921 cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag
    34981 aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac
    35041 tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt
    35101 caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa
    35161 cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca
    35221 aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc
    35281 cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga
    35341 taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg
    35401 ttgtatctgt ttcttttttc actctttttc ctcctttatc tataaattca ttatagacaa
    35461 aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata
    35521 taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa
    35581 attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc
    35641 aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc
    35701 atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt
    35761 aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc
    35821 gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc ccacgattat
    35881 gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat
    35941 gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat
    36001 acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat
    36061 gaccatgaac acggggaaga aggccataca catgagctag acccacatgt atggctagct
    36121 ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca
    36181 gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc
    36241 catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat
    36301 gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt
    36361 tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa
    36421 catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta
    36481 gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa
    36541 caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa
    36601 aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc
    36661 gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc agattgggca
    36721 ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttgaa
    36781 tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag
    36841 ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt
    36901 aagaatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac
    36961 gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct
    37021 tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat
    37081 ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca
    37141 ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag
    37201 gcaaaaaaga accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt
    37261 tctttttatg aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt
    37321 gccaacgtga cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca
    37381 taggatttat gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca
    37441 tagaattgta attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa
    37501 gtagaaaaat attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt
    37561 ttgaagatat ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata
    37621 tgctcgacat tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg
    37681 atgttgcttt ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt
    37741 cctaaaggat ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct
    37801 tctagttcga tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg
    37861 agaagttctt ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg
    37921 attctatttt tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata
    37981 taaatgggaa tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa
    38041 cgctggtttt caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc
    38101 aagataaaca agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt
    38161 ttcaactggt ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa
    38221 tgattcttct tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca
    38281 atcgcattgt ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc
    38341 agttcgattg gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg
    38401 agttttaatg acagtaaact acggatctca ggaactttca tattatccaa catacttaga
    38461 ttggccggaa aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac
    38521 cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg
    38581 taatcatgtt tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc
    38641 tgttgcatcc tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc
    38701 caataataaa ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc
    38761 aaagctaaga catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat
    38821 gtaaataata tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa
    38881 gtctctaacg atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca
    38941 atgtgaaaat actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa
    39001 tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg
    39061 taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata
    39121 gcaattagag cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa
    39181 atcagaatga ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata
    39241 caaattgtgt aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc
    39301 cattgatttt taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat
    39361 tttgaattca gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt
    39421 taattgttta atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg
    39481 taataggaag caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt
    39541 aagaagctga gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct
    39601 ttaataatgg gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat
    39661 agcgaagcag taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag
    39721 tcaattgcag atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca
    39781 gacaaacgaa ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag
    39841 aaagaaaggt gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga
    39901 ccgctagttt tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca
    39961 ccatatcttc ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt
    40021 tatctttcat ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt
    40081 cttttgtttc ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct
    40141 tcgttttcca agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat
    40201 gacaagtttg tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg
    40261 atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta
    40321 aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg
    40381 tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact
    40441 ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag
    40501 agcataaaga ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa
    40561 acagcttttc atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg
    40621 tttatttgaa aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg
    40681 agttactttt ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt
    40741 tatttctata aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat
    40801 aattgtgatc attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct
    40861 tgcccaagat ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc
    40921 gctattttat tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa
    40981 acgctttttg gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat
    41041 ctacgaagat agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga
    41101 agtaaagcag tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc
    41161 acaaaagaaa ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag
    41221 aatcaaaaaa agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta
    41281 ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa
    41341 aatagtgatc gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat
    41401 cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat
    41461 ccgctacact acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa
    41521 ctaagcaaat tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt
    41581 gattcattga gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt
    41641 ctgagtgagg gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa
    41701 cagatgatga cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag
    41761 atagtggaaa aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt
    41821 cgttgctacc ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat
    41881 tttatcaaaa gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc
    41941 gggatgctca tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag
    42001 aacaaacaag cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt
    42061 tagaagaatc cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca
    42121 ttctccgtca aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa
    42181 agcaccatat tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag
    42241 tcctattgta cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata
    42301 tttttgtctt ccttatctta gcataaacga ttgaaaaaaa ggaaaaattt tcagaaaact
    42361 aaaatcatat cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata
    42421 gacaacgaaa ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa
    42481 ggaaatcaat cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg
    42541 ggagaacaag tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac
    42601 ctatgaagcg gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga
    42661 aagtgaagga aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa
    42721 tcaagaacaa aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg
    42781 agctgggagc ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa
    42841 acaagtagac gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact
    42901 aaaaaaacga gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca
    42961 tagtatcttt tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca
    43021 aggaatacaa ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca
    43081 gctgatacgt aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc
    43141 tcggttagac tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa
    43201 agaaaaatat gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg
    43261 attgctcacg gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt
    43321 actccaaaat ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa
    43381 atatgattat ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt
    43441 gaaacggatg acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag
    43501 tatctatgga ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc
    43561 taccaaaact cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc
    43621 aaatagattg atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca
    43681 aaaagaagca ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg
    43741 gctaatcaaa caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat
    43801 attataccga tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat
    43861 tccttttgta ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca
    43921 acatttctat caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca
    43981 ggcctaccag caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg
    44041 cgatcaatac ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat
    44101 aagccccaat ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa
    44161 aacagaccag catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat
    44221 agctaaggaa atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc
    44281 tactttcaga agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa
    44341 aacccgcttg attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca
    44401 caaaagtaaa ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt
    44461 gccttataac ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc
    44521 agtaacgacg atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa
    44581 tcgtctgtat ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt
    44641 tatcaaagaa accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag
    44701 aagtggtcag tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg
    44761 aaatttcagc ttattttcga agaagctatt tctggaacac agtttattcg gaaaagggag
    44821 gtgtgacaag aattttgtca cacctccctt atttattttg aaaatgagaa tactattatc
    44881 aaaatagaga atatatagaa aaattctcat tattcaaacg taaataatca gttattttga
    44941 ttttttttat attttattag ctaataattg gtattttaaa agaaattact gtagaattgg
    45001 attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta gctgtacttc
    45061 ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag agaagaatat
    45121 ttttgccgaa ccgttaacag aaagtgagat caaaaagatc ttggcagcga ctgaaggagg
    45181 agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca tagattttaa
    45241 cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat tattgagacg
    45301 acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg agatccggtc
    45361 atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac aaaacaataa
    45421 catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga tcagcgcact
    45481 gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct tatcaatttg
    45541 ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc agttttgaag
    45601 aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac attgcgattg
    45661 acctttcctg tgtaggaaaa aaacaactga tttttttatg cacacaacat accaatctcc
    45721 tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat gagcagttga
    45781 tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca ctgagcattt
    45841 catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct ttttttgact
    45901 ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa tctaggtggg
    45961 gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc atttctgttg
    46021 ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag ttgttgacat
    46081 gttagaaatg acatgataag ataactgagt tgttaaaaaa gaaccacttg tgaagcttga
    46141 ttctatcgaa aaaaacttca aaaaagttct tgacaaataa caaatgataa gttatgataa
    46201 atgagttgct gtaagatttc gaaaaacgaa atcaaaaaaa cttttaaaaa agagttgaca
    46261 attacttgtc tctctgatat gatataaaag ttgctacggc agccaaaaac gaatcgggga
    46321 acactcctga tgaagtagac ctttgaaaac tgaacaaagt aagacaaacc aaatgtgtag
    46381 ggcgtcttga ttcaattcaa gacaacaaac a
//
LOCUS       DACEZN010000004        45855 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEZN010000004 DACEZN010000000
VERSION     DACEZN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.004"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(165..1475)
                     /locus_tag="IXH60_000151"
     CDS             complement(165..1475)
                     /locus_tag="IXH60_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1989124.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1487..2905)
                     /locus_tag="IXH60_000152"
     CDS             complement(1487..2905)
                     /locus_tag="IXH60_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1989125.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3145..4713)
                     /locus_tag="IXH60_000153"
     CDS             complement(3145..4713)
                     /locus_tag="IXH60_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1989126.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4717..5127)
                     /locus_tag="IXH60_000154"
     CDS             complement(4717..5127)
                     /locus_tag="IXH60_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1989127.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5124..6047)
                     /locus_tag="IXH60_000155"
     CDS             complement(5124..6047)
                     /locus_tag="IXH60_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1989128.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6044..6922)
                     /locus_tag="IXH60_000156"
     CDS             complement(6044..6922)
                     /locus_tag="IXH60_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1989129.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6909..8246)
                     /locus_tag="IXH60_000157"
     CDS             complement(6909..8246)
                     /locus_tag="IXH60_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1989130.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8249..9352)
                     /locus_tag="IXH60_000158"
     CDS             complement(8249..9352)
                     /locus_tag="IXH60_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1989131.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9426..10934)
                     /locus_tag="IXH60_000159"
     CDS             complement(9426..10934)
                     /locus_tag="IXH60_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1989132.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10900..11592)
                     /locus_tag="IXH60_000160"
     CDS             complement(10900..11592)
                     /locus_tag="IXH60_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1989133.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11614..12588)
                     /locus_tag="IXH60_000161"
     CDS             complement(11614..12588)
                     /locus_tag="IXH60_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1989134.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12888..13760
                     /locus_tag="IXH60_000162"
     CDS             12888..13760
                     /locus_tag="IXH60_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1989135.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14218..16416)
                     /locus_tag="IXH60_000163"
     CDS             complement(14218..16416)
                     /locus_tag="IXH60_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1989136.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16648..17640
                     /locus_tag="IXH60_000164"
     CDS             16648..17640
                     /locus_tag="IXH60_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1989137.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17700..19121
                     /locus_tag="IXH60_000165"
     CDS             17700..19121
                     /locus_tag="IXH60_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1989138.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19114..20613
                     /locus_tag="IXH60_000166"
     CDS             19114..20613
                     /locus_tag="IXH60_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1989139.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20652..22046
                     /gene="uxaC"
                     /locus_tag="IXH60_000167"
     CDS             20652..22046
                     /gene="uxaC"
                     /locus_tag="IXH60_000167"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1989140.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22205..22888
                     /locus_tag="IXH60_000168"
     CDS             22205..22888
                     /locus_tag="IXH60_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1989141.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22890..23726
                     /locus_tag="IXH60_000169"
     CDS             22890..23726
                     /locus_tag="IXH60_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1989142.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23737..25491
                     /locus_tag="IXH60_000170"
     CDS             23737..25491
                     /locus_tag="IXH60_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989143.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25767..26108)
                     /locus_tag="IXH60_000171"
     CDS             complement(25767..26108)
                     /locus_tag="IXH60_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1989144.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26176..28347)
                     /locus_tag="IXH60_000172"
     CDS             complement(26176..28347)
                     /locus_tag="IXH60_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1989145.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28558..29415
                     /locus_tag="IXH60_000173"
     CDS             28558..29415
                     /locus_tag="IXH60_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1989146.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29486..30343)
                     /locus_tag="IXH60_000174"
     CDS             complement(29486..30343)
                     /locus_tag="IXH60_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1989147.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30328..33030)
                     /locus_tag="IXH60_000175"
     CDS             complement(30328..33030)
                     /locus_tag="IXH60_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1989148.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33043..34332)
                     /locus_tag="IXH60_000176"
     CDS             complement(33043..34332)
                     /locus_tag="IXH60_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1989149.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34482..35528
                     /locus_tag="IXH60_000177"
     CDS             34482..35528
                     /locus_tag="IXH60_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1989150.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35530..37683
                     /locus_tag="IXH60_000178"
     CDS             35530..37683
                     /locus_tag="IXH60_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1989151.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38156..39607)
                     /locus_tag="IXH60_000179"
     CDS             complement(38156..39607)
                     /locus_tag="IXH60_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1989152.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39604..41328)
                     /locus_tag="IXH60_000180"
     CDS             complement(39604..41328)
                     /locus_tag="IXH60_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1989153.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41321..41935)
                     /locus_tag="IXH60_000181"
     CDS             complement(41321..41935)
                     /locus_tag="IXH60_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1989154.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42280..43737)
                     /locus_tag="IXH60_000182"
     CDS             complement(42280..43737)
                     /locus_tag="IXH60_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1989155.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43778..44698)
                     /locus_tag="IXH60_000183"
     CDS             complement(43778..44698)
                     /locus_tag="IXH60_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1989156.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44710..45657)
                     /locus_tag="IXH60_000184"
     CDS             complement(44710..45657)
                     /locus_tag="IXH60_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1989157.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
BASE COUNT        13563 a         9375 c         8074 g        14843 t
ORIGIN      
        1 tttttaacga cttagttatc ttatcacgtc taaaaacatg tgtcaactct tttttggaat
       61 tttcataaaa tgtttcaatg aagcgcgaca acgttgttta atatatcatt aaaaaaaata
      121 aaggtcaagc taaaatatta tttaagcttg accttattat aatttcaacg attgtcaaaa
      181 gtattgtttg cagctgtgtt gccagtttct atagataaat cacagctttt gaaacataag
      241 ttttgtactg cttcgattcg cccgaattct tttgcagtta tttggcaatg agaaaagcag
      301 acatcttcta taggcttttc aggaaatgct actaaatcaa aagctcggga aggaagtgag
      361 taacctggag aaaggcatgc ctgtacgttt ttgatgtgta tgtctttgac tttcgtcatt
      421 tgctttgatt caggtatttc ttcagccact gcttgccaac tttctggcat ttgagcaagg
      481 tcattgctat tttttctatt gtattcatta tgccagtcca ttatccaaga aaaaggaaat
      541 tgtacatcaa tcatattcag attttctacc ctgatatttt cgatcactcc ccctctttct
      601 tttgaagatt tcattcgaat accacagtct gactgaaaaa aatcgatatc gtgaatatat
      661 acgtcactaa ctccagcact aacttcgcta ccaatagtta caccgtatcc tgaacggatt
      721 ttacatctag caacctcgat tcttgatgat tttcgattga ctcttgcacc attgccatct
      781 ctgccagact taatagcaat acaatcatca ccacaggcca aatcacattc atagacacgc
      841 acatttgttg aagaatcaat atcaatccca tctgtacttg gtccatcatt gttgcggatg
      901 attacttttt ctactaatac atcgttagaa taagtgattt gcaagttcca aaaacctgat
      961 ttctgaaaaa tcaaatcacg aacaacaaca tgttctgcat gatacagtaa acaagcgcgt
     1021 ggacgtttaa tagcataatc cgcaatccaa cgaagtccct tttgatcgta ttcagctctt
     1081 gtcccgcttt tttgatcttt tccccaatac agctcccacc aatggtctcc ttgaccgtcg
     1141 attattcctt ttccttctat aagaatatct ttcgccgaga gaacattcaa aatcgctgct
     1201 ggccatttca tttctacgcc ggctactcgt gtatcgattt ctggaaatgc ttcgatatct
     1261 gttctcccct tgaggactgc accttcttca aagataagat gactcttatc ttttaaaaaa
     1321 agggctccga ctaaataaat gccttttgga ataaatacag gtaagtgttc tttcgcacct
     1381 tgatcgattg ctttttgcag tattattgta tcatcttgtt gagtgtcgtt tgttttaaag
     1441 ttcaaagcat tgataatccc tttttttgtc cgcaatacgt ttctccctat ctttcattga
     1501 tttttttcaa ttcttcacca ataaatacag tttttgaacg attcagtggg taacctacta
     1561 gcaaaatgat tgcggataca aataagcaga atgctggtaa caagtttgcc atcgtataaa
     1621 tcctttcttg tactaaaact gattggatgg ctccacctgt tgtagacgat tggtaaccaa
     1681 tgagtgccag cataaatccg cctaccgctc ctgcacatgc ttgtcctact tttctcgcaa
     1741 aagaatttac tccatatact gttccatctt ctcttaagcc tgtcttatat tgatgataat
     1801 caatcacgtc agtaatgaat gcccagacca tcaaattgaa taaaccagat cctaaagtag
     1861 cgataaataa aagaatcaga tatacccatg catttgttat atgaaggaaa tacatcagca
     1921 agtacataca tgaagaagca agtaacgcaa atacgcttgc ttccttttta ccaaataatt
     1981 ttgtcagctt tgtagcaaat ggtgctaaaa ctactactgt tccgtaagta aataaaagag
     2041 caacagacat tgcttgttta ttatggaagt aatcattgaa taaataagtc atattcgtcc
     2101 cagctaaaat ctgattgatc acaatgaaaa tgtcaacaat gactaaaata atcagtgctc
     2161 gattagctaa caaacctttt accaactgag aagcagatac tttttcgttt ttgacaactc
     2221 ttactctttc agtagtcaat ttacaagtta acatatatgc gacaaatgct attgcagcac
     2281 atccgattga aatccaaaag aaatgattcc ctgaaacgac ttgttgtcca ctcttgttat
     2341 gggtatacat taaaatcgga atgaagaatc ctgtagttga tccgccaatc gcagaaccaa
     2401 tgcttcggaa cgtggacaac gaggtgcggt cattaggatt tgagctaatt gctgctgcca
     2461 ttgctccata cgggatattg attgttgaga gaaaaatccc ataaacaatg tatgaaacaa
     2521 aaatataagc aatcttcacc gtcattggaa gatgttggac gaatggcaaa aacaaaatga
     2581 cagtcactaa gcaaaatgga tacttcattt ttctgatcca aggaataaat cggccagttt
     2641 ctgttaactc tgagacatca cataatcttc caaccatcac atcagcaaac gcatccaaaa
     2701 ttctcgaaat caagaataac gtacctacga ttgcccctga aacgccaaga acattcgtat
     2761 aataaatcat caagaaacta ttgactaaac ctaaaataaa acagttacct agatcaccga
     2821 acatgtagcc tatcttatcg gaaatcccaa attccttttt ttcttttttt attgtatttg
     2881 ctgtaacagc attttgtgaa agcatttgaa ttcctcctac ctcttctagt aaacgttttc
     2941 aaaattataa ctcttttttt cttgttttta tattcacaat gttggctttt tattgcatta
     3001 tcttgacaga taaaaaacaa aaaataatct aaaaacattg atgcataaga ttgataactt
     3061 tttttacata acaatatata caaacaaaaa aattcactaa ctagtgaaaa taagttagtg
     3121 aacctcattt attaaagaat taaattacag atgatctttt ccatcatact tgaaaaactc
     3181 aaaatcagca taacttcctc tttttttatt tagatcatgc actgctatcc cgataaaatt
     3241 tccagtaaac cctcctgaga gtatacacat gtttttttgc ggacctactt tcttccattc
     3301 ttcagttgat acgtctttca aatgaaactg tgctgtctct ttctttacag tgactttcaa
     3361 aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct atttgtgcta ctccgttttc
     3421 tgattctatc atacgtaggc attttccctt gtcctcattc caagtaacat aacaataaat
     3481 ataattggat tcatttaagt acagtaccag tccagccatc tgattaaaat tattaggcat
     3541 gtattcaaat gctgtctccg catcaaaagt aaaatccgtt tgccgaatag ctaacaaatg
     3601 atgttcgaag gtagattgaa gagattcacc ggaaatcaga cgtaaaaaac ctggtctaca
     3661 ttttgtatca caccaatcag catttggcaa aatgcgtctg gcattccagt tcttctttag
     3721 ctctccttca aatgtatcca taaactgatg atctttttct tgttgtacct cttcatttgt
     3781 ctcaatcatt atttcttcca tcggagttgt ctggctatct ttaagtctta accaatcgtc
     3841 ggacgtccaa tacacttctt gaatagcggt ttctcttccc aaaatagccg caccgtttaa
     3901 tggtctggta caaagatatg tcatgtacca tcttcctgac ggggtttgta ctaagctccc
     3961 atgtcctgta cattgcaatg tagaagcagg tttgtcacta gctgtcagca taggaaaacc
     4021 aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg caaactgtaa ctgaatgtcc
     4081 tcttcctgta ccgccttctg ccgtcatcaa ataataatac ccgttacgaa aatacaaatg
     4141 tggtgcctcc gttttcgcta attctgtacc tgcaaatatt ttatagatgg gaccatcaag
     4201 cttcttcttc tctgcatcat attcttgtaa tacgataccg ctggatttat tcggtgtgtc
     4261 cagacgataa tcccatagtt cattgacaag ccattttttt cccgtttcaa gatcatggaa
     4321 caaagaagga tcaaagccgt tactatttaa ataaattggt tcggaccaag gacctgtgat
     4381 ctcagatgca gtgatcaaat aattgtggac atctttaaac ggacggcctg cacttttgac
     4441 atctgtgtaa acaagataaa acaatccatc agcataactg atctgtggcg cccatatact
     4501 tccatttgta ggatttcctt gcaaagatac ttgagtggtc aaaatatccg ttgtatgtga
     4561 ccagttgact aaatttttcg actcatatac tcggattcct ggaagccatt caaaagagga
     4621 gacaataatg taataccgct ctttcactct taatatatta ggatctggat tgaatcctct
     4681 taaaatagga tttttcactt tgatagtctg catgattcag gctccttttc tcattcttac
     4741 tcgatataaa ctgaatgccc cagctgcgaa ataaaaaaat gcaaaaggaa acgcaataaa
     4801 ctgagtgcca atcaacaata tgaatcctgt tataaaatac aaaccagtgg cagttgttaa
     4861 gtattttcct ttagcagtca aaaacaatgc tactgctaaa aaagcaagtg aaataaacaa
     4921 cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt ctaaagacaa
     4981 agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg cagacatatt
     5041 acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac cgaccattcc
     5101 tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa atgtatccat
     5161 tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt gacagtctgt
     5221 tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc acctatctta
     5281 cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata gacgtttcta
     5341 gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt ttgatttttc
     5401 ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg gatatgacac
     5461 tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc aatccagcaa
     5521 tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca aaaaggagta
     5581 ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata accagatagt
     5641 cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc ctgtcctacc
     5701 ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac attcgatcct
     5761 tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag atactcctgt
     5821 tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt aaaattggat
     5881 aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac ctttggatgg
     5941 ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa atccgtcggg
     6001 gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa tgctccctta
     6061 tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct gttaaagaaa
     6121 aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc ccttttctca
     6181 gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag ctaagacgag
     6241 ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat agatttggca
     6301 catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtatt tcgatgtcca
     6361 cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc acccaccagc
     6421 ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct gtgtcttccc
     6481 ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc cataacgttt
     6541 ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg cgttcttcaa
     6601 atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact tctgcaaaag
     6661 atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact gaaaaatctc
     6721 cgccgtgaga atgcaggtaa aggaccggcg gaaacggtcc ttctttttct ggataagcat
     6781 agagggctgg tactgtttcc aattcattta agtacaattg atagtattca agcataaaac
     6841 cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta gatggagcac
     6901 ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc atccatattt
     6961 tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc taatagaaat
     7021 gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc cgcaaagcca
     7081 atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg aacaccggga
     7141 actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg tatgaattgt
     7201 tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt gatattcgaa
     7261 aaatggatcc ttctgaatgc tggtgtccgt tcatctatcg gataagcctt tttttcccat
     7321 acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat aaatggacac
     7381 atcacattat ccatgactat attggatact cgaatatctt caacgatccc gcctcgacct
     7441 cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt tgaaatggta
     7501 atattacgaa tactaccact catttcactt cctaatacga caccgccatg tccatggacc
     7561 atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt atcttcagtc
     7621 cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact aatacgcaca
     7681 tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg gttcaagatc
     7741 gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca acttggtgac
     7801 tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact catcaattta
     7861 ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca tttttttcca
     7921 tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg agcatagata
     7981 caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac aggataatct
     8041 tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc gacattgctt
     8101 tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt cccgcctccg
     8161 tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc atcaattgat
     8221 gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc aatgcttgca
     8281 agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg tagtaagcat
     8341 atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt ttattcaagt
     8401 tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga tgtgatttct
     8461 tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct ttagccattg
     8521 cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt tttgtttctg
     8581 ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat gcagccagtg
     8641 tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt aattcatagg
     8701 tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga tgggatagac
     8761 ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga atcagcaaat
     8821 ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa acatcttggt
     8881 aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa cccatatata
     8941 agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct ccttcggatg
     9001 tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta tatttttctt
     9061 cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg tcgatattgt
     9121 attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag tcgatattgt
     9181 cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat tggaaccctt
     9241 taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct tcatataagc
     9301 gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc attatttgct
     9361 ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg tcccaatcgt
     9421 ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat tcgatcgcct
     9481 ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag ctgtcccatg
     9541 atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac ttcattgttc
     9601 gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt ttctttgatc
     9661 aattgattag agaattcttt ttcagggtct gtatctttga aagagaacgt cacttcacta
     9721 ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc gataaattcg
     9781 atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt catcaattta
     9841 tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa ttgtgcttcg
     9901 tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc ttctgaccaa
     9961 acatgataac cgccgccttc accatctgag atcttgttta caggcactaa ttccatatca
    10021 tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc agtgtagatt
    10081 ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac tgcaaagtct
    10141 tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt catatactct
    10201 tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc atttggtgct
    10261 ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg cgcacgatcg
    10321 ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt aaattgttta
    10381 gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt atccaaccag
    10441 tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac gccgtacaag
    10501 gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa atttttatag
    10561 tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt gcggacagaa
    10621 gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc agaagccaat
    10681 gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt cagttccgta
    10741 tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc atgatgcaaa
    10801 atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact gttacttgaa
    10861 ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa ttttacgagc
    10921 gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt tttcttctgt
    10981 tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg ctgtccacgt
    11041 gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca ttgtttggat
    11101 gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat gaagatgtga
    11161 gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat cataatgagc
    11221 catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa tatacaaagc
    11281 gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa tcccgatggc
    11341 gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat atgtgaagta
    11401 cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca ctgcaaccat
    11461 cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta aaccgcccca
    11521 cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta ttttatctaa
    11581 tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt ctcctagctt
    11641 tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt taaacaatcc
    11701 cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa tataggtgtc
    11761 gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat gctcgaaccc
    11821 taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg gacgaattgc
    11881 cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt ctgcagcttc
    11941 atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag accagcccaa
    12001 ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg atgttagaaa
    12061 actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc cgtcaacatt
    12121 caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag ggatgtaaat
    12181 gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata gtaaagacaa
    12241 aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca acgtatttct
    12301 taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga aaccgaccca
    12361 aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa cgagtccccc
    12421 cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca tcacatataa
    12481 aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct tttcttcttt
    12541 tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa gcccctcctt
    12601 agttttgtga atgtttgctg cttatgactt catgatagat aactatctga attacgtata
    12661 taatcatttt taccgtaagt aaaaacgctt acatagcaat gatttttgtg ctatacaaaa
    12721 cgataaagtg tattttttca caaataaact acgcatagca aaccttctat ttttttctat
    12781 ctgacactta gcataggaaa tgattatata cgtaatcttt caccgatgat aaattgaaca
    12841 tatagaaaca gaaacattta atttattttg aggaggagcc tatctatatg aaaaagaaag
    12901 tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt ttgctagctt
    12961 tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct acacaagaag
    13021 tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg tatcgctaca
    13081 ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt acaggtgtcg
    13141 gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc agaaagtatt
    13201 tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc agcgggggga
    13261 tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac tgggccatga
    13321 tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt ttccaagcct
    13381 tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt ggtgtatttt
    13441 tcaaaattat gctgccatta gcgttaccgt ctattgctac tatctcgctt ttttatgctg
    13501 ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca tctaaatggc
    13561 cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa gcagaaagta
    13621 gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc gttattgcaa
    13681 cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa ggagcactcg
    13741 taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg tcagaagtgg
    13801 tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt tgtgaaattt
    13861 cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt gtgaaagaga
    13921 cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta acccacagga
    13981 gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat gccttattgt
    14041 cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca agacttttgt
    14101 cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac aaatactagg
    14161 gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc tcttgattca
    14221 caaatatttt tttcgatatt ctcccggcgt aatcgattcc ttctttttaa agaaacgaat
    14281 aaaattttgc ggatttcgat attgcaatct ttctgcaatc tcttttacgg agaggttagt
    14341 ctctttcaac catttttttg ctacctctaa acgatgattt tgaacaaaat caccaaattt
    14401 ttctcctgtt tcctttttaa atacattact taaatagtta gggttataat gcaacctatc
    14461 agcgatcaca tctaaagaca gatcacgatc atattctgtg tagacgatat gcataatatt
    14521 ctccgacaaa tttttaaatt catgatcagt gattgcttga gagcattctg tgattggcag
    14581 aactaattgt tctaaaacaa gtttttctag ttctttggga tgataattgt tcagtgcttt
    14641 tacataaatt tgtttcatgt tatcaaagtt ttttgaatct actcccaata actgtcctaa
    14701 ttgaacgagt tcattgatca agcggaccga aactacttcc cgactcaaag gatttttatt
    14761 ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc ttcacttcct gatttccaga
    14821 acgaatggct tcaaacaatc gattttgttc ttctatcgga tatctaataa gtgttttttc
    14881 atgagcagca ggaacgattt cctgataaaa aataattgaa cttggaccag tatttacacg
    14941 ataatacagg gcttcttttg cccgatcaac cgattgcttg ctttcattca gcgtttcgaa
    15001 ccggttactg atgcctatac tgatagttaa attcaagtat tcttttacac gtttttgaat
    15061 gagtcggcaa tactccatga cttttttgct tgcttctgca tcatctttat ccattcgata
    15121 aatagtggct tgcatttctt cattcaaaac gatcggcagc attcgatttt cctttggcac
    15181 aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc caccgtgact
    15241 atcttctaac agatcgattt gaaccaaagc tgtataatag cagtcatttt gagaactata
    15301 accaaattgc tgcaaccgct gatctagttc tcgcctttct actcgatttc taaataaact
    15361 aagtacaaat agcgtttcca gctgtggctt ttgggtaact aagtgtgcac ttaacgcttc
    15421 tttttctcct atgatccgac tgactgattg agcaaccaaa tctagttctt tcccactgaa
    15481 tccttttgat ttaagattca gacgttcctg tatttgagaa atcggacggg caaatctgtc
    15541 cgaaaataag taggaaagaa gaccaatgag tcctaccagt gttaaagaca ctgcaactag
    15601 cccgattctc agattgcgga tcattgcgcc aattgcacta gagtcaactt ctaatctgta
    15661 aatccaatta ttataatctg atttcagtac tatatacgtt ttatcttttt ctcttatcac
    15721 tttcagctga ttgtaatcta gatccacgtc cgttaataca gttggttgct ctttattttt
    15781 tactgtctga gtagtaaaaa ggttgccaac ttcgttttca atagaaagta tgtcttctcc
    15841 actttcacca acaacttgtt ctattgtatg tttctctatc gtagcaattc ccaatgcata
    15901 tttttctttt tcaaacattg gcaaagaaat caccatttcg attcctttgt ttttcttttg
    15961 ccaaaataaa ttatttttgt tatttatgta cttctgttga tacttttcaa tttcctcttc
    16021 ggtcagttga ctcaacgatc cattgattac tccccattta ccagccaaac tgactaaatc
    16081 ataattatta tcatccataa caatcgtttc tataaaattt agttctttcc tgatttcaga
    16141 ataagtggag taatctgtat atgtcagcgg atgcttgaat atgtccgaga aatttttggt
    16201 cgaaccatac gtagaaaacg aatattcaat cgttctaatt ttccattcaa cgttagtttt
    16261 aacttgcctg agcaaattct ttttgtctgt taaataactc gattcgatcg attcataggt
    16321 attctggtaa atgaataaac taaatcctat gacaagtacg gctcctaata aaaacatcgg
    16381 taaaaatatt ttataaaata ctgtactttt cttcattttc ttcctctcct ccttttaaaa
    16441 taaaaggctt tcatttttct gtaaaattga tactcatcta attaaatgag cattccccgc
    16501 tttcaagaat agcataatta aaaataaaaa tccaagtaat ttttaacgtg tgcaatcttt
    16561 tcatatcaaa gatctattga cttatcgtag ttatcgtaat atgatgaatg cgttaacaaa
    16621 aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca aagatgtcgc taaagcagct
    16681 ggcgtttctc atacgacagt ttctcgtgct ttgaataaca gcagtttaat aaaaaatgaa
    16741 acaaaacaaa aaatacgaca actagctaag gaaatgaatt attcgccaaa ctatagtgca
    16801 aaaggtttag tgaatcaaaa aaaatatcta atcggactat ttttctcaag tttgcataaa
    16861 ggaacttcat caaatttcct agcagattcc attgcaggca tccattcagt attagacaca
    16921 aattacagtt tatctgttga aagtatcgat gttgttgatt taacagaaat caacctgcaa
    16981 cgctacgatg gactgatcgt tatgagtcaa agtgatgatg atcagccttt catcgactat
    17041 ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga tccagcggtt
    17101 atcaacgtca ctgccgatga tgcggccggt gttatgcagg cagtagatta cggtattgga
    17161 tgcggacata cgaagatcgc ttatattggc ggaaagaaaa attttcgttc gacaaaagaa
    17221 cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa tccagcctat
    17281 tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata cctattatca
    17341 ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat tggtgcattg
    17401 agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat cgggttcgat
    17461 gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa gcctttgaaa
    17521 gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag gacgatcgaa
    17581 caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa ttacttgtaa
    17641 actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag gaagtttata
    17701 tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa gttcttcagt
    17761 ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag ctgaataacc
    17821 aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt cttggaaata
    17881 tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc aatggcgaaa
    17941 tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct tatgatgaat
    18001 gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc tccaatacaa
    18061 cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc ccacagagcc
    18121 tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa gctggcttta
    18181 ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa atcattttac
    18241 gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa aaagaaaatg
    18301 ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa gatgcaggtg
    18361 ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa ccattcatgc
    18421 tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa caggctggct
    18481 tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg catttgctta
    18541 atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa actgtagaag
    18601 aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat ctagaattga
    18661 ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa atcaaagaac
    18721 gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat agtgtttcaa
    18781 agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa aatcaggtac
    18841 caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc gatcaagtaa
    18901 agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct gataatggaa
    18961 cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg tctcaaatac
    19021 cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa ggtgtccggt
    19081 cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca accaacaatg
    19141 aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa acaaacacaa
    19201 ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg tcataagatt
    19261 gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc aatcggtcat
    19321 gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa aaccaatctt
    19381 tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta cctttttgag
    19441 aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg taatgatttg
    19501 tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt caaacagttc
    19561 aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa gcatccttat
    19621 ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc ggacgcagta
    19681 aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa caatacagtt
    19741 cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt tctagtggct
    19801 caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct tttaacagcg
    19861 gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg tttgaaatgc
    19921 ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc attttctgac
    19981 tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat gtttggcgca
    20041 gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat cgttgacttg
    20101 atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga aaacccatct
    20161 cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg ctgcacgcaa
    20221 aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat cagaaaacca
    20281 gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc tcttgcttca
    20341 tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg ctcatatgtc
    20401 ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca ttggatggat
    20461 tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc gtttgttcaa
    20521 aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga cgttcgagaa
    20581 attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat aaaaagaaag
    20641 aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg gcgaaaaagc
    20701 tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac ttagatccaa
    20761 aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta ggaggcgatc
    20821 attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc acaggaaatg
    20881 cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt atcggaaatc
    20941 cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac ttgttgataa
    21001 atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca gctcctgatt
    21061 ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca acagatgatc
    21121 ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat ttgaaggttc
    21181 taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt aatgcatggg
    21241 tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa ctgatagaag
    21301 tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc gaccacgctt
    21361 tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt tttcaaaaag
    21421 cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcgtaca gaaacactga
    21481 tacatttgac ccacttctat cattctcata attggacgat gcagctgcac atccatgctt
    21541 atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc tatgatggaa
    21601 tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca gaagaaacgg
    21661 atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct gcgattttag
    21721 ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt tctggatggt
    21781 ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct gatggtagtg
    21841 ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc tacactagac
    21901 atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg cgaggagaag
    21961 cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat acaaatgcta
    22021 aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa tcaaaaacag
    22081 gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg accaaatttg
    22141 ctccgtcttc ttctcttgct gttctgcttc attgcctata aacctttatg aaagaagaga
    22201 taacatgcct actatccata ttgccggtga ttctactgcg gcagaaaaag atgaaaacaa
    22261 gcgtcccgag acaggttggg gagaaaaaat ctctgcttat ttttcacccg agatccagat
    22321 caataaccaa gcaaagaatg gacgcagcac caaatcattt atccaggaag gaaggttagc
    22381 ccgtctagct tcacagtttc agccgaatga tttcttattt gtacaatttg gacataacga
    22441 ccagaaaatc caagaagaaa gaggtacgct tcccttcggt agctatttaa aaaatctgca
    22501 gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga catctgtgac
    22561 gcgacgagat tacttagaaa acggaacatt gaatccagat atacttggag actatccaaa
    22621 agcgatgcgt gcatttgctg agaaacacca tattcctctt ttagatgtgt tctcaagatc
    22681 acaagaattg ttccagacct tctcaaaaga agagacaaaa aaattctacc tgcatcttat
    22741 acctgatact tgtaaaaatt acccagaagg actaaaagac aatacccact ttagtccaga
    22801 aggcgctgat aaagttgccc gaataattgt tgaattgata aaagaatccc gtctgcctct
    22861 tgctaattat ttacaaaaag gagtttgaga tgtttccatt aaatttaggc atccgtgccc
    22921 acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag cttcacttgt
    22981 cccatatcca atttgcacca caaaaagcat ttcctgaaca acttaaatta actgacatga
    23041 cacttgggag tgctgcctat tttggtgaat acttccaaaa aaacaatatc gaactttcca
    23101 ttttaggatg ctatatcaac ctatccagca aaaatcaaga agttcgtgca caagcattgg
    23161 caactttcaa acagcaaatt gctctgtgct ctgcctatca agccaagatg attgctacgg
    23221 aaaccggtag tgtctctgtc ggttacacaa aagaaaattt tactgaagaa gcttatcaga
    23281 tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga aaattttggc gtaactgttg
    23341 ctgttgaagc agggatcaat catccaatct ataactatca gttagctaaa cgtttaatcg
    23401 atgaagtagc ttcaccaaat ttaaaaatca ttttagattg tgcgaatttg atgcataaag
    23461 aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt ggacaattta catggttata
    23521 ttacagcgct tcatctcaaa gactatatca tggaaaatgg aaagatccgc atggtacctg
    23581 ttggcaaggg ctgtttagat ttccgcccgg tactgcatta tatcaaatat gagcgtcctc
    23641 taatgtacgc cactcttgaa gctactcctg agatttatgt tcctgaagca atagaacact
    23701 tgcagtttat ttaccaagat tgttaaggag aagattatgg aggaacgaat acagaaaagt
    23761 tatcaaaaat atcatgcaaa tgcttttcag acattgctgc atctttatca gggaaattat
    23821 tggcgttttg ccatgtcagg actctttttt attatcaaac atcttccatc ttggttaatg
    23881 ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga aagattcttc tcagttgcag
    23941 gccatttatt tcaacgcttt tttgatggct tgtctgattg gtcaaaatat attgacgaat
    24001 tattttcatg taaaatacca tagcacttcg atccgtcaag tagaagctgg actaagagcg
    24061 acccttatca agaaaatcca agaactatct attccttatc aaaaagaatt gcaatctgga
    24121 cgaatccagt caaagatcat ccgtgatgta gaagctatcc aaacgttgtc ctctcaagta
    24181 tttgtcagcg gattaaatat cgctgtcaat ttattcgtag ctttaggtat cacgctttat
    24241 aaaagtcctg ttgttttcat gtttttctta ttgactgttc ctctcgccgc attcgttact
    24301 ctctttttcc gtaaaaggat cagacagtcc aactcagact tcaggcaaga aatggaagag
    24361 acttctgcaa aagtgatcga agtgatcgaa ttagcaccga ttgctcgagc tcatgggtta
    24421 gaaaaacacg aaacaactcg cttaaataag cattttactc aaatctatga aaaaggattc
    24481 cacttagata tcctgcaatc tgttttcgga tcagtcagct gggctagttt tcagatattt
    24541 cagctgattt gtttgatttt cactgggata ttagctttac aagggcgcat ccaacccggc
    24601 gatgtcgtga tgtatcagac gtatttcacg acgatcgtca attccgtttc tggaatgatc
    24661 acgctgattc ctacgatttc aaaaggcatg gaatctatca cttctattgg cgaactgttg
    24721 attgctggcg atgtagaaac ttatcacggt aagaaaaaaa tccaacattt ggatggaaat
    24781 tttcgttttg aggatgtttc ttttctttat ccggactcac tcaaaccgat tatcgatcat
    24841 ttttctctag aggtgaaagc tggagaaaca attgcttttg ttgggccatc tggctctgga
    24901 aaatctacac tccttaactt attgattgga tttatccagc cgacttcagg aaaaattttt
    24961 ctagatgatc agcctttgga tgaactggat atgcgaagtg tgcgaaatta tttagccgtt
    25021 gttcctcaaa ccactttgtt gttctccgct tcgatcaaag agaacatcac ttatggcttg
    25081 aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag ctgcgcaact ttcttcactg
    25141 attgaccagc ttcctgatgg tctggatacc ttagtaggcg aacatggcaa caagctgtct
    25201 ggtggccaaa agcaacgtat ctccattgca cgagcgttga tacgtcagcc aaaaatcatt
    25261 ttgctagatg aagcaacctc agctttagat aaccagtcag agaaaaaaat ccagcaagcc
    25321 ctgaattacc tgacagaaac acccactact tttattgtgg ctcatcggct ttcaacgatc
    25381 aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa tcgttgaaat aggtacttac
    25441 gaaaaactgt tagctcaaaa aggctatttc taccgcttga aaaatgaatg acaaaaaata
    25501 ggagctatga caagactttt gtcgtagctc ctatttctga ataaacggtg ttctcctgcc
    25561 aactcctcgg tcacaagtca caatagtcat caaacatgag aagctgtttt ttgaaattgt
    25621 tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg tgttcaagag
    25681 acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg tctctttcac
    25741 accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac tggtgtcatg
    25801 atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg catcaagtca
    25861 ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat tgcatcttcg
    25921 tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat cgcttggaag
    25981 tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc ttctttcaac
    26041 gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct atcgtagatg
    26101 tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac atggggttat
    26161 tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag ccgcttcacc
    26221 aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt cttttgcttg
    26281 ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag aatttcctgt
    26341 gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat ctgctacact
    26401 aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga agactttccc
    26461 gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt cgtaacccat
    26521 ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat ttgcttttgt
    26581 ggcatcgaaa ttagtttctg tggttcctgt tttccctgct atcgtataac ctgcaggatt
    26641 cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca tttcatcggc
    26701 tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga tcaccgctcc
    26761 agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat cattggcaaa
    26821 tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc caccaagggc
    26881 taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg ctttatcata
    26941 tcctttttgt aaaccgatct catgcaaaag ccatacagta ggcaaattca aactttgtgc
    27001 aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat gcgcatcgta
    27061 ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt ccagtgctgg
    27121 tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct gtgtagcaaa
    27181 gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta caccacctgt
    27241 attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag catttgctgg
    27301 gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct gatctaaagc
    27361 tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat actcgttaac
    27421 tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat ctgaattatc
    27481 gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt cttgttcttt
    27541 tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat cattcgcatt
    27601 ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg ccaaagtagc
    27661 agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg aagcgtcttg
    27721 gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca agatttcttc
    27781 tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt ttcgatcaaa
    27841 cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg tacttcctcc
    27901 gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa tccctttgat
    27961 atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg catcttggat
    28021 atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca gtgtgccagc
    28081 ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt ttaacgtttc
    28141 gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga cacagatcaa
    28201 accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct ttcgccatcg
    28261 atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag cagcttttat
    28321 cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg attcaatcgt
    28381 cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt caatatgttt
    28441 ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa gttttttctt
    28501 aaatatgttt taaaaatggt acaattagtc tgcgattgca ctgaaaggag tacacttgtg
    28561 aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga cttgcttgaa
    28621 aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa aaacgtttgg
    28681 atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca gatcacattg
    28741 ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc gaagcacatc
    28801 cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg aatgaagact
    28861 catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc ttatctcgac
    28921 actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag cggtgccgtt
    28981 ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga acaaaaaatg
    29041 atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat gaccatcacc
    29101 aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga taaaaatggt
    29161 cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac acaagtctat
    29221 tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca taagggacat
    29281 ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat gctccatgca
    29341 ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa agctttacct
    29401 ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat gataagactc
    29461 tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct aacagcaaac
    29521 taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca cttacttgag
    29581 gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt tgaaggagat
    29641 gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac gtcaacgtat
    29701 agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga tgatcttgcg
    29761 gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct gctaatcctt
    29821 ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga tggcgtttca
    29881 cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca ttgatcacga
    29941 cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt ccttgtgctg
    30001 ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa gcagcaggaa
    30061 tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct tttttgggag
    30121 ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg ccgaatgagc
    30181 caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga tccaatgttt
    30241 cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca tctgaaaccg
    30301 cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa
    30361 tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc
    30421 ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa
    30481 atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa
    30541 agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt
    30601 tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa
    30661 ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc
    30721 acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact
    30781 gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa
    30841 gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg
    30901 cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa
    30961 aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg
    31021 atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct
    31081 gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg
    31141 ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg
    31201 gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt
    31261 tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa
    31321 gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg
    31381 tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg
    31441 gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc
    31501 ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa
    31561 agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata
    31621 accaaattgg acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc
    31681 tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc
    31741 ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat
    31801 tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat
    31861 tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt
    31921 ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc
    31981 gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt
    32041 tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg
    32101 gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata
    32161 ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac
    32221 atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg
    32281 aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc
    32341 aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt
    32401 ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata
    32461 ccaattggca aatagcagac caaagattgc tgtttgatca ggtcctttcc accacatctc
    32521 tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt
    32581 tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg
    32641 tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg
    32701 gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc
    32761 atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg cttgttggat
    32821 tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc
    32881 caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa
    32941 ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct
    33001 atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat
    33061 atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct
    33121 cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc
    33181 agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc
    33241 ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc
    33301 ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt
    33361 ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt
    33421 gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata
    33481 aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc
    33541 tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat
    33601 ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata
    33661 acggcaagta tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt
    33721 tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt
    33781 ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc
    33841 ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc
    33901 caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt
    33961 catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc
    34021 atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc
    34081 taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct
    34141 taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt
    34201 gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg
    34261 gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc
    34321 tttataagcc actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca
    34381 tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta
    34441 tggtattatt tgaatatgat atatcattaa gaggtgttta tatgaatcaa ccattgtacc
    34501 agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc
    34561 aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac
    34621 gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt
    34681 tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc
    34741 tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc
    34801 ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata
    34861 aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag
    34921 aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag
    34981 ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg
    35041 cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta
    35101 cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca
    35161 aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc
    35221 tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta
    35281 tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta
    35341 tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac
    35401 aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa
    35461 aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg
    35521 agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat
    35581 ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca
    35641 ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa
    35701 ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt
    35761 acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc
    35821 ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc
    35881 tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat
    35941 gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac
    36001 acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat
    36061 tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat
    36121 agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca
    36181 tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa
    36241 gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc
    36301 acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta
    36361 tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact
    36421 agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa
    36481 acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt
    36541 tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt
    36601 ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc
    36661 cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc
    36721 aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct
    36781 aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt
    36841 agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca
    36901 ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat
    36961 ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt
    37021 gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt
    37081 ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac
    37141 ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga
    37201 caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga
    37261 caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct
    37321 ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat
    37381 agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta
    37441 attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct
    37501 aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacaaaga atatacgaaa
    37561 ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc
    37621 atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat
    37681 taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat
    37741 agatcctcat atttttcgtt tggttggtct tattaccgca gatgcaagct tttgaacacc
    37801 gtttattgag aggttgtgaa atcagcgttc tgacctgagt attagagaga caaatggcaa
    37861 aatagttcct catattttgc cattttttga gctaataccg agggtcagct gattgaacac
    37921 cgtttagtgg agaggtcgtg aaaaagctgt ttagtttcga gtattaagaa aaattttgtg
    37981 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    38041 ctccgtttat cgagaggtca tgaaaaagct gccttgcctc aagtaataag aacagcccaa
    38101 aattagattt ctagacaatt ttttataagt tatttcatta cattggcttt ttcttttatg
    38161 attctccact gttttctcgg tactctttag gggatctgcc gacgattttc ttgaataatt
    38221 tagagaaata cgtggtgttg ttgtagccaa tctcacttgc tatctcattg atgttttgat
    38281 tactgtgcaa aagcagttgt ttcgcttttt ccatacgtaa atggttcaaa taagctgaaa
    38341 aacttttttt cgtttctttt ttgaacagct gtcctagata catgacattt aaatgcaggt
    38401 tttctgacac ttctttgagt gtaagtggtt cttgataatg ctggtgcagg attacgatta
    38461 ctttttccac attgtcactg tactttttct gtctctgatg ctttttgatg aatgacagaa
    38521 gtagctgctg aagttcttgc accgtttttg cctgatggat tttttcgatt ccttgtaaat
    38581 attcctcatc ttccagatca atcaattccc gttgaatctc cataaacagt aaaaaactgg
    38641 aatatcgtat atcttccggc atcatggctg ctaactgaaa ctgttcaaaa aaatcagtga
    38701 tcattttttg agcgacttcc aattgtttat tttgcagcac gcgtctgaaa cttgaaaaat
    38761 cgatggtttg atcttttgtt tctgcttgta ctgcgtaaaa aacatgctcg tctttatctc
    38821 cataaaattg atgcaactgt agattgtctt tcgcttgttg gaatgtttca gggattttgt
    38881 ccaaagaatc tgtctcttcc ccgatactga tcagccattc ctgatcagga aagttttctt
    38941 gaacaaaaca acagaagcca tcaacttcat ctgcctccaa caacaacgtg atcaatacac
    39001 ggtctccgta atttcgttga tagtaaaaga tttgttgccg ttcttgcaac catctattga
    39061 taagagtatc atgagttctt gccagttgaa tcaatactat cctatgctgc ttctcactta
    39121 tttgtcctat tatttcttct tcacttattt catccaattc ttcattcaac cattgactga
    39181 ataacagttc ttgatagatt tcttgttgat ttttttgttc attttgctga tctaaccgtg
    39241 ttttgatttt cttgaggctt tcaaccaatt cgaacttatt cacgggtttc atcaaataat
    39301 tcacggcgcc taattgcata cctcctttca aataatcgaa ttcttggtat cctgataaaa
    39361 tcataaattc aaaattatga cgttcttttt gagccgcttc aataaattcc agtccattta
    39421 tttctggcat tgtcacatcc gttactacta aatcaatctg tcgctgttca agtacagcta
    39481 gtccttcttt ggcattctct gctgttgcta ctatttgaaa gcctaaactt tgccaatcaa
    39541 ttatcttttt taacccattc aaaatcatgt attcatcatc taccaataac acttgataca
    39601 cgctcatccc tccttgaatg taatagaaac ggtcagtcca cctgtctcat tattctcaag
    39661 cgacatataa taactgctgc cgaagctggc acgtaaacgt tcattcacgt tttgcagacc
    39721 gatagaaccg tgaagttcca ctttaggatg agacagtttt tcttcaatct gttttagccg
    39781 tttttcagtc atcccttttc cattatcttt gatgatgatc cttacccgtt tgccttcctg
    39841 caagactttg acacttagcg cattatcgaa tcgagtaaaa tcaataccgt gtttaaaata
    39901 attttcaact aatggctgga tcacaaattt aggtacttcg atcttttcca aatcaggatc
    39961 gatcataaaa tgataggcta ctcgattagg ataacgcatt tgatacaaat agacatattt
    40021 ttcgcaaaag tccagttctt cttttaaagt gatcgttttc tcttgattcg tattgtttcg
    40081 aagcaaagcg gaaaacgcat acacaacatc tgctaattct tcacttcctt cgctgattgc
    40141 atacattcgg atatattcta atgtattata taaaaagtgt ggattgatct gtgcttgaag
    40201 cgcccgcata tgtgcgtctt gctgtttgat ttctaatttg tagatgtctt ctacaaactg
    40261 attgatgcta tccaacattt cgttgatacc gattgaaagt tccttcagtt catattccgt
    40321 gtttgttatg tcgatccgcg tctctaaatt ccctgctgct gtttgatcca tggcctgcat
    40381 gacgatagat acttgctgtg aatatcgttt gaatgtttta taaagaaaga taagcagtat
    40441 attgtctaaa ataatgatac caatgatcag cggggcaaga ttttttcccg tttcgatttt
    40501 tacacttttt ttatctagta cagctaatat acgataatca tcagacagtt cttggtgttg
    40561 aacaaaattg tttttttcca gatcgtcaat cggcaattgt gaatttttct tcagactctc
    40621 tgtgatgatc atttcttgtc tttcaaacgt ttctttagct attttattat catggaatgt
    40681 gtatagtcga tttgtcgttc cagaaatcat gtataaagag agtccattga atgacgttag
    40741 attttctaaa atcatatcta gttctttctt ttgaaaacca ataaaaatac ttcctgactc
    40801 agctcctcct tgtgtgatag gcaacttgat ataaaaggcg tctagtaatt ttggtgtccc
    40861 cttttttatt tccccacctt tgttttctct agaagattca taatactccg gtagttgatt
    40921 caacacgata tatatcgcac ttagttcttc ataatttgcg tagagatctt tgatttgatt
    40981 tgggaaagaa actaattcat cggtactgtt ttgctgttcg tatacataca tcagatactg
    41041 atctatgggt tttgttaagt aggtcgtcac attttcgatc ttttcggaat tccctacaag
    41101 ctggtttttg atgatcctac tcaaggttgt tttatcattc agcattctgc ttgtgctttg
    41161 aacggcatca cttgtcatgt tttctgcctg tttttgattt tgttcccaag tatggatact
    41221 aaaaataacg gtagcagtca tcgttacacc gatcatgata accgcgtatt tccgcaacag
    41281 acgatttagt aaggttcctt ttgaaaatag aatccgaaac ttattcataa ctttccaact
    41341 gtttatctaa ttcttcacgc caatgtgttg tcatcgtacc attccaaacc gtcaaaagac
    41401 caatgacacc aaacaggatc aatcctttga attgccaagt cacgcctaaa attataccgc
    41461 taccaataat tatttttaga aaagacgaaa agctggaaaa aaccgaaata aaacttagct
    41521 ttagcagatt aggaacagag atcgtatatt cactatctaa aatcatactg tattgataag
    41581 tagcaaatag gcagagcaag gcaaaaagta aaataaaatc aatagctaaa aaaatgagcc
    41641 ctttgatttg aacagataaa tataaattgt aactcaataa agcagtacca gataaaaata
    41701 aactaaatag cagattgcct cttaaaaaat ttcttttata tatattccag cctcttttta
    41761 ttgtgatttc tttatactcg aatccgtaca aataaaatag ttcattgact gttttccatg
    41821 ccgcacctaa tccaaagatg atcccgccgg caaatgtata tatccagaaa tacaaggtta
    41881 gttttataat gacccagatc cggataaata actcttccaa aaccttccct aacatatctt
    41941 ctcctccaaa cattaagttt atactttctc attatacaaa aaaaggttgg gcaatcagca
    42001 ccttgacctg attattagag aaacaaacgg aaaaatagtt cctcatattt tgtcgttttg
    42061 tgagcgaata ccgagggtta gctgattgaa caccgtttac caagagtttg tgaaatagcc
    42121 gcttagtttc gagtagtaag aaaaattttg tgaatatggc tttccacatt tttacatata
    42181 tttagcttaa taccgagaaa ctggcttttg aacaccgttt attcggaaca agaggttgtg
    42241 acacgtcttt tgtcacaacc cctttgtctt tattcattat tattcttgtc ggaactcatc
    42301 atattgtttt tgcatttctt tcaatacctt atcatagcca gccttgtcca aagcgtcttt
    42361 caatttcggt aatgtttcat ctggatcaac agtccctgta ttcaatccat ctaaatattg
    42421 attcatgaca ttagaaatat tacttaattc tgttttgaca ctgtcagtgt tgaagctgaa
    42481 tcctaaaatc ggtgaagttt ctgcatcagc aatcgattga tcacgtttgg caatcatgtc
    42541 atccgtaata gactcttgtg tatatagaat cttgttgttt cctgtattcc aagcagacat
    42601 atgtgtattt ggctgataac catctagcaa tttgatttta tcatctgaac catcgacttt
    42661 ttcccatgct tctccctcga tcccccaaac aagcccattc aataattctg gatcactgtt
    42721 aagcagccct agaagttcta ctgatttttc tttatttttt gaagtatttg atacgacaaa
    42781 atttgccatt tgtgcttgag ctgtagattt tagtggaaca gtaattgctt ttgagacaag
    42841 ttcttgtttt gctgcatttg tcaaaattgt atcgccataa tcatacggtc cttgtgtttc
    42901 tccacgcatt agccaagtat ttccttctag tggataatcc ttattgctcg ttgcagcgtc
    42961 acttggaatg agtccttgtt tgtaccagct atgcattgta cgtaacagtc cgatatacgt
    43021 ttcattatcg tattggttga tgattttcga atcatctcca tccagatcaa tagcaaatgg
    43081 taacgtattc cctaaaggat aatcaaagtt cccttggact ttgaagcctt gtccaatggc
    43141 aaaagctgcc gtgtttggtt ctttttcatg gaattccttc aagacttttt ctgcatcagc
    43201 gtaagattgg attccatcaa ttgaaagatc gtatttatct agtaaagctt tgttaaaagt
    43261 aagtacttgt tgagcaaaga catttccatt gactgggaaa gcatataatt ttccattgac
    43321 cagatttcct ttaatatatg cttcatctaa atcattataa gctttttcag cgtacttagg
    43381 cgctaattca gtcaaatcag caaaggctcc tttttgggca ttggaaacat agttgttcgc
    43441 aaaggcgata tcataatttt ccccggaaga aatgatgacg ttcattttct tttcgtaatc
    43501 gccccagcca atgtaattga tattgacttt tacaccgatc tctttttcga ttcgtttgtt
    43561 tgctacttcc atcaattgat cgaaattatc tggtttatct ccgatttgat acatctgcaa
    43621 agtgtccgaa ttgctagctt ctgcctggtc tttagaacca gaacctccgc atgctgctaa
    43681 tgttcccaac aacaacgctg aaacacctgt aaaggctagt ttcttccata acttcatttt
    43741 tcttcctcct aattttatga tttatatttt tgtttcttta ttcttttact ccgccgattg
    43801 ttagaccttt gacaaaatgc ttctggaaaa atggatagct gatcgcaata ggcaacgtcg
    43861 agatgactac gatcgccatt ctggcagatt ctcctggaac tgaggctaat ccgcctgtca
    43921 attggcttcc tgctcctgaa ttttgtgtca gatattgaat attactttgg attttcatca
    43981 aaagatactg tagtggaact agcttatcat cttggatgta tagtaaagca ttgaaccagt
    44041 cattccagta acccaatgcg gcaaataaac tgattgtcgc aatacctgga acagctaacg
    44101 gtaaaacgat ctgaacaaat attcgcagtt cgctagctcc atcgatccgt gccgattcaa
    44161 taatcgaatc agagaccgaa cgtttaaaga atgtgcgcat cacaataata ttgaagggac
    44221 tgacagccat tggcaagatc aatgcccaaa tcgtatcttt taactgaaga agatttgtca
    44281 taacaagata attggccacc atccctggtg agaacaacat cgtaatcaaa caaaaaacgg
    44341 taaaaaaacg acggaatgga aagtttgacc gtgaaatcgc ataagcatat aaagaagtag
    44401 ccgtgctatt gatcagtgta cctaaaacag taacaagtac agtgacgaaa agcgcttgga
    44461 aaattttatc ctgcatctgg tcgaacaagt acgtataacc aaatatactg aacttttctg
    44521 gccaaaaact gtacccgtga gtcgacaata tggactcatc ggtaaaagag atggcgataa
    44581 caaatataaa tggcaaaata caggaaatag caaagagtgc aatcattata ttagagaaaa
    44641 aattagccgt cttattgaaa gagcggactt ggactctctc cactttcttt tttttcattt
    44701 ttttcctcct tagaataatg cagaatcgct atcaaaacgt cgggcgatcg tatttgtgat
    44761 catcaacaag acaaagccga caactgattg gtaaagtcct gcagcagccg tcataccaaa
    44821 atctcctgtg gatgtcaggc cattgtagat atacgtatcc aaaacttgag tcacatcata
    44881 taatgcaccg gaattcctag gaatgttata aaacaggcca aaatcagctc ggaaaatgtt
    44941 tcctacggct aaaatcgtta aaatcgtcat tagtggaata agttgtggta tcgtcacatg
    45001 tttgatctgc tgccatttgc tagctccatc aaccatagca gcttcataat aagtcggatc
    45061 gattcccata acagatgcaa agtaaataat gctgttataa ccaatccctt tccatgtacc
    45121 aatgaacacc aagataaacg gccaatattt cggttcatta taccaattga cagcttctcc
    45181 tccatgtcca gtgatccact gattgaatat tcctttgtca ggacttaaaa aagcgtaaac
    45241 aaagtaactg ataatgaccc aagatagaaa ataaggtagt aaggacatcg tttgataaac
    45301 tttaacgagt cgtttatttc tcaactcgct cataatgatt gcaaatacga ctgagatcag
    45361 cagattcaaa aggataaatg ctacattata taaaattgta tttttagtga tgagaaatgc
    45421 ttgatccgaa gaaaataaaa atttgaaatt ttcccaaccc acccaaggac tttccctcaa
    45481 gctagccaag aacccatctg ctgaaatatg aaaatttttg aatgctacga cattcgccat
    45541 tacaggtatg taaaagaaaa aaatcatcca aagcataccc ggtacagcca tcaggacaag
    45601 tgctttataa cgccatagat tcataaaaaa atgattgaac ccctttcgtt ttttcatcta
    45661 aaaacccctt tcgattcatt cgcaacgtaa ttatagcgct ttcaataatg agaataaata
    45721 aacaaagtat agggaaaatg aaacaaagta taggagaggt tgtgaaatca gcgttttggc
    45781 ctgagtatta gagaaaaaaa cgggaaaata gttcctcata ttttgccgtt ttttgagcta
    45841 ataccgaggg tcagc
//
LOCUS       DACEZN010000005        44082 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEZN010000005 DACEZN010000000
VERSION     DACEZN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.005"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(72..449)
                     /locus_tag="IXH60_000185"
     CDS             complement(72..449)
                     /locus_tag="IXH60_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989158.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            705..1433
                     /locus_tag="IXH60_000186"
     CDS             705..1433
                     /locus_tag="IXH60_000186"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1989159.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1433..1687
                     /gene="purS"
                     /locus_tag="IXH60_000187"
     CDS             1433..1687
                     /gene="purS"
                     /locus_tag="IXH60_000187"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1989160.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1689..2360
                     /gene="purQ"
                     /locus_tag="IXH60_000188"
     CDS             1689..2360
                     /gene="purQ"
                     /locus_tag="IXH60_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1989161.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2361..4583
                     /gene="purL"
                     /locus_tag="IXH60_000189"
     CDS             2361..4583
                     /gene="purL"
                     /locus_tag="IXH60_000189"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1989162.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4568..6007
                     /locus_tag="IXH60_000190"
     CDS             4568..6007
                     /locus_tag="IXH60_000190"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1989163.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6039..7082
                     /locus_tag="IXH60_000191"
     CDS             6039..7082
                     /locus_tag="IXH60_000191"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1989164.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7079..7657
                     /gene="purN"
                     /locus_tag="IXH60_000192"
     CDS             7079..7657
                     /gene="purN"
                     /locus_tag="IXH60_000192"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1989165.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7654..9189
                     /gene="purH"
                     /locus_tag="IXH60_000193"
     CDS             7654..9189
                     /gene="purH"
                     /locus_tag="IXH60_000193"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1989166.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9205..10479
                     /gene="purD"
                     /locus_tag="IXH60_000194"
     CDS             9205..10479
                     /gene="purD"
                     /locus_tag="IXH60_000194"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1989167.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10601..11515
                     /locus_tag="IXH60_000195"
                     /pseudo
     CDS             10601..11515
                     /locus_tag="IXH60_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            complement(11648..13213)
                     /locus_tag="IXH60_000196"
     CDS             complement(11648..13213)
                     /locus_tag="IXH60_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1989168.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSYFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13206..14657)
                     /locus_tag="IXH60_000197"
     CDS             complement(13206..14657)
                     /locus_tag="IXH60_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1989169.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14671..15687)
                     /locus_tag="IXH60_000198"
     CDS             complement(14671..15687)
                     /locus_tag="IXH60_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1989170.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15907..16995
                     /locus_tag="IXH60_000199"
     CDS             15907..16995
                     /locus_tag="IXH60_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1989171.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17049..18575
                     /locus_tag="IXH60_000200"
     CDS             17049..18575
                     /locus_tag="IXH60_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989172.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18577..19782
                     /locus_tag="IXH60_000201"
     CDS             18577..19782
                     /locus_tag="IXH60_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1989173.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20039..21016
                     /gene="guaC"
                     /locus_tag="IXH60_000202"
     CDS             20039..21016
                     /gene="guaC"
                     /locus_tag="IXH60_000202"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1989174.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21280..22704
                     /locus_tag="IXH60_000203"
     CDS             21280..22704
                     /locus_tag="IXH60_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1989175.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23165..24274
                     /gene="pdhA"
                     /locus_tag="IXH60_000204"
     CDS             23165..24274
                     /gene="pdhA"
                     /locus_tag="IXH60_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1989176.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24277..25254
                     /locus_tag="IXH60_000205"
     CDS             24277..25254
                     /locus_tag="IXH60_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1989177.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25279..26922
                     /locus_tag="IXH60_000206"
     CDS             25279..26922
                     /locus_tag="IXH60_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ1989178.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26930..28336
                     /gene="lpdA"
                     /locus_tag="IXH60_000207"
     CDS             26930..28336
                     /gene="lpdA"
                     /locus_tag="IXH60_000207"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1989179.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28498..28773
                     /locus_tag="IXH60_000208"
     CDS             28498..28773
                     /locus_tag="IXH60_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1989180.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28779..29546
                     /locus_tag="IXH60_000209"
     CDS             28779..29546
                     /locus_tag="IXH60_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1989181.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29714..31543
                     /gene="typA"
                     /locus_tag="IXH60_000210"
     CDS             29714..31543
                     /gene="typA"
                     /locus_tag="IXH60_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1989182.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31804..33576
                     /gene="recQ"
                     /locus_tag="IXH60_000211"
     CDS             31804..33576
                     /gene="recQ"
                     /locus_tag="IXH60_000211"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1989183.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33690..33992
                     /locus_tag="IXH60_000212"
     CDS             33690..33992
                     /locus_tag="IXH60_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1989184.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33995..35158
                     /locus_tag="IXH60_000213"
     CDS             33995..35158
                     /locus_tag="IXH60_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1989185.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35184..38612
                     /locus_tag="IXH60_000214"
     CDS             35184..38612
                     /locus_tag="IXH60_000214"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1989186.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38633..39757
                     /locus_tag="IXH60_000215"
     CDS             38633..39757
                     /locus_tag="IXH60_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989187.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39747..40184
                     /locus_tag="IXH60_000216"
     CDS             39747..40184
                     /locus_tag="IXH60_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1989188.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40168..40506
                     /locus_tag="IXH60_000217"
     CDS             40168..40506
                     /locus_tag="IXH60_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1989189.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40625..41185
                     /gene="rsmD"
                     /locus_tag="IXH60_000218"
     CDS             40625..41185
                     /gene="rsmD"
                     /locus_tag="IXH60_000218"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1989190.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41182..41673
                     /gene="coaD"
                     /locus_tag="IXH60_000219"
     CDS             41182..41673
                     /gene="coaD"
                     /locus_tag="IXH60_000219"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1989191.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41663..42706
                     /locus_tag="IXH60_000220"
     CDS             41663..42706
                     /locus_tag="IXH60_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1989192.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42865..43593
                     /locus_tag="IXH60_000221"
     CDS             42865..43593
                     /locus_tag="IXH60_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1989193.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43709..43954
                     /locus_tag="IXH60_000222"
     CDS             43709..43954
                     /locus_tag="IXH60_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1989194.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43951..>44082
                     /locus_tag="IXH60_000223"
     CDS             43951..>44082
                     /locus_tag="IXH60_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1989195.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     "
BASE COUNT        14592 a         7559 c         9818 g        12113 t
ORIGIN      
        1 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
       61 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      121 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      181 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      241 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      301 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      361 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      421 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      481 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      541 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      601 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      661 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      721 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      781 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      841 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      901 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
      961 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1021 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1081 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1141 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1201 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1261 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1321 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1381 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1441 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1501 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1561 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1621 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1681 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1741 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1801 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1861 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1921 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     1981 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2041 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2101 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2161 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2221 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2281 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2341 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2401 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2461 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2521 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2581 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2641 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2701 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2761 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2821 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2881 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     2941 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3001 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3061 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3121 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3181 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3241 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3301 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3361 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3421 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3481 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3541 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3601 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3661 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3721 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3781 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3841 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3901 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     3961 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4021 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4081 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4141 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4201 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4261 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4321 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4381 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4441 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4501 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4561 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4621 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4681 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4741 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4801 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4861 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4921 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     4981 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5041 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5101 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5161 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5221 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5281 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5341 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5401 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5461 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5521 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5581 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5641 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5701 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5761 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5821 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5881 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     5941 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6001 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6061 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6121 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6181 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6241 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6301 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6361 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6421 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6481 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6541 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6601 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6661 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6721 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6781 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6841 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6901 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg
     6961 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7021 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7081 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7141 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7201 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7261 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7321 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7381 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7441 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7501 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7561 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7621 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7681 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7741 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7801 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7861 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7921 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     7981 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8041 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8101 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8161 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8221 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8281 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8341 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8401 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8461 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8521 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8581 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8641 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8701 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8761 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8821 acaaaacgta agccgactcc agcagaagca aaagcattag tgtttgcttg gagagctgtc
     8881 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     8941 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9001 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9061 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9121 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9181 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9241 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9301 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9361 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9421 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9481 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9541 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9601 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9661 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9721 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9781 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9841 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9901 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
     9961 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10021 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10081 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10141 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10201 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10261 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10321 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10381 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10441 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10501 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10561 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10621 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10681 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10741 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10801 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10861 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10921 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    10981 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11041 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11101 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11161 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11221 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11281 gaactaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11341 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11401 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11461 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11521 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11581 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11641 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11701 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11761 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11821 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11881 aataagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    11941 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12001 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12061 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12121 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12181 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12241 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12301 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12361 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12421 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12481 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12541 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12601 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12661 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12721 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12781 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12841 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12901 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    12961 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13021 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13081 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13141 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13201 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13261 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13321 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13381 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13441 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13501 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13561 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13621 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13681 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13741 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13801 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13861 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13921 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    13981 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14041 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14101 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14161 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14221 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14281 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14341 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14401 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14461 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14521 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14581 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14641 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14701 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14761 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14821 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14881 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    14941 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15001 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15061 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15121 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15181 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15241 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15301 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15361 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15421 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15481 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15541 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15601 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15661 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15721 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15781 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15841 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15901 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    15961 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16021 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16081 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16141 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16201 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16261 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16321 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16381 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16441 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16501 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16561 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16621 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16681 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16741 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16801 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16861 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16921 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    16981 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17041 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17101 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17161 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17221 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17281 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17341 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17401 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17461 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17521 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17581 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17641 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17701 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17761 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17821 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17881 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    17941 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18001 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18061 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18121 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18181 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18241 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18301 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18361 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18421 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18481 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18541 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18601 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18661 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18721 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18781 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18841 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18901 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    18961 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19021 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19081 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19141 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19201 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19261 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19321 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19381 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19441 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19501 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19561 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19621 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19681 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19741 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19801 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19861 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19921 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    19981 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20041 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20101 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20161 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20221 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20281 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20341 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20401 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20461 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20521 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20581 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20641 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20701 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20761 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20821 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20881 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    20941 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21001 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21061 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21121 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21181 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21241 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21301 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21361 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21421 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21481 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21541 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21601 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21661 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21721 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21781 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21841 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21901 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    21961 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22021 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22081 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22141 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22201 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22261 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22321 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22381 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22441 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22501 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22561 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22621 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22681 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22741 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22801 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22861 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22921 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    22981 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23041 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23101 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23161 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23221 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23281 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23341 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23401 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23461 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23521 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23581 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23641 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23701 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23761 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23821 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23881 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    23941 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24001 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24061 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24121 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24181 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24241 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24301 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24361 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24421 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24481 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24541 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24601 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24661 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24721 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24781 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24841 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24901 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    24961 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25021 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25081 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25141 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25201 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25261 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25321 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25381 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25441 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25501 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25561 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25621 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25681 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25741 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25801 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25861 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25921 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    25981 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26041 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26101 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26161 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26221 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26281 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26341 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26401 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26461 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26521 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26581 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26641 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26701 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26761 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26821 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26881 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    26941 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27001 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27061 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27121 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27181 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27241 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27301 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27361 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27421 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27481 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27541 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27601 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27661 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27721 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27781 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27841 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27901 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    27961 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28021 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28081 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28141 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28201 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28261 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28321 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28381 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28441 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28501 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28561 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28621 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28681 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28741 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28801 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28861 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28921 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    28981 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29041 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29101 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29161 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29221 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29281 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29341 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29401 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29461 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29521 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29581 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29641 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29701 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29761 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29821 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29881 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    29941 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30001 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30061 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30121 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30181 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30241 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30301 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30361 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30421 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30481 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30541 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30601 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30661 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30721 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30781 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30841 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30901 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    30961 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31021 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31081 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31141 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31201 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31261 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31321 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31381 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31441 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31501 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31561 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31621 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag gcgtttaaaa
    31681 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31741 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31801 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31861 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31921 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    31981 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32041 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32101 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32161 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32221 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32281 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32341 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32401 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32461 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32521 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32581 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32641 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32701 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32761 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
    32821 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
    32881 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
    32941 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
    33001 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
    33061 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
    33121 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
    33181 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
    33241 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
    33301 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
    33361 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
    33421 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
    33481 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
    33541 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
    33601 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
    33661 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
    33721 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
    33781 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
    33841 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
    33901 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
    33961 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
    34021 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
    34081 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
    34141 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
    34201 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
    34261 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
    34321 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
    34381 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
    34441 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
    34501 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
    34561 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
    34621 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
    34681 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
    34741 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
    34801 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
    34861 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
    34921 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
    34981 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
    35041 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
    35101 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
    35161 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
    35221 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
    35281 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    35341 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    35401 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    35461 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    35521 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    35581 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    35641 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    35701 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    35761 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    35821 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    35881 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    35941 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    36001 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    36061 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    36121 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    36181 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    36241 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    36301 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    36361 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    36421 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    36481 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    36541 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    36601 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    36661 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    36721 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    36781 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    36841 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    36901 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    36961 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    37021 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    37081 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    37141 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    37201 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    37261 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    37321 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    37381 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    37441 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    37501 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    37561 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    37621 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    37681 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    37741 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    37801 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    37861 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    37921 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    37981 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    38041 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    38101 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    38161 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    38221 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    38281 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    38341 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    38401 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    38461 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    38521 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    38581 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    38641 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    38701 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    38761 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    38821 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    38881 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    38941 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    39001 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    39061 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    39121 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    39181 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    39241 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    39301 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    39361 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    39421 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    39481 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    39541 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    39601 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    39661 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    39721 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    39781 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    39841 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    39901 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    39961 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    40021 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    40081 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    40141 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    40201 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    40261 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    40321 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    40381 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    40441 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    40501 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    40561 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    40621 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    40681 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    40741 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    40801 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    40861 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    40921 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    40981 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    41041 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    41101 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    41161 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    41221 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    41281 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    41341 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    41401 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    41461 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    41521 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    41581 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    41641 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    41701 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    41761 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    41821 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    41881 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    41941 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    42001 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    42061 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    42121 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    42181 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    42241 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    42301 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    42361 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    42421 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    42481 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    42541 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    42601 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    42661 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    42721 agaggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    42781 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    42841 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    42901 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    42961 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    43021 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    43081 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    43141 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    43201 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    43261 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    43321 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    43381 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    43441 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    43501 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    43561 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    43621 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    43681 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    43741 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    43801 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    43861 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    43921 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    43981 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    44041 aataacaagt tgatcaaaga atatatctct gttctcacag aa
//
LOCUS       DACEZN010000006        42589 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEZN010000006 DACEZN010000000
VERSION     DACEZN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.006"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            1077..2687
                     /locus_tag="IXH60_000224"
     CDS             1077..2687
                     /locus_tag="IXH60_000224"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1989196.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2979..3848
                     /locus_tag="IXH60_000225"
     CDS             2979..3848
                     /locus_tag="IXH60_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1989197.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            4013..5284
                     /locus_tag="IXH60_000226"
     CDS             4013..5284
                     /locus_tag="IXH60_000226"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1989198.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            5281..6576
                     /locus_tag="IXH60_000227"
     CDS             5281..6576
                     /locus_tag="IXH60_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1989199.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6675..6938
                     /locus_tag="IXH60_000228"
     CDS             6675..6938
                     /locus_tag="IXH60_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1989200.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            7383..7832
                     /locus_tag="IXH60_000229"
     CDS             7383..7832
                     /locus_tag="IXH60_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1989201.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7889..9307
                     /locus_tag="IXH60_000230"
     CDS             7889..9307
                     /locus_tag="IXH60_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1989202.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            9307..10167
                     /locus_tag="IXH60_000231"
     CDS             9307..10167
                     /locus_tag="IXH60_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1989203.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            10341..11115
                     /locus_tag="IXH60_000232"
                     /pseudo
     CDS             10341..11115
                     /locus_tag="IXH60_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            11262..11627
                     /locus_tag="IXH60_000233"
     CDS             11262..11627
                     /locus_tag="IXH60_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1989204.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11775..12983)
                     /locus_tag="IXH60_000234"
     CDS             complement(11775..12983)
                     /locus_tag="IXH60_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1989205.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(13002..13280)
                     /locus_tag="IXH60_000235"
     CDS             complement(13002..13280)
                     /locus_tag="IXH60_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989206.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13473..14129
                     /locus_tag="IXH60_000236"
     CDS             13473..14129
                     /locus_tag="IXH60_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1989207.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(14571..15368)
                     /locus_tag="IXH60_000237"
     CDS             complement(14571..15368)
                     /locus_tag="IXH60_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1989208.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(15365..16549)
                     /locus_tag="IXH60_000238"
     CDS             complement(15365..16549)
                     /locus_tag="IXH60_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1989209.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16574..17821)
                     /locus_tag="IXH60_000239"
     CDS             complement(16574..17821)
                     /locus_tag="IXH60_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1989210.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            18036..19781
                     /gene="dnaX"
                     /locus_tag="IXH60_000240"
     CDS             18036..19781
                     /gene="dnaX"
                     /locus_tag="IXH60_000240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1989211.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19801..20115
                     /locus_tag="IXH60_000241"
     CDS             19801..20115
                     /locus_tag="IXH60_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1989212.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            20140..20736
                     /gene="recR"
                     /locus_tag="IXH60_000242"
     CDS             20140..20736
                     /gene="recR"
                     /locus_tag="IXH60_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1989213.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20800..21444
                     /locus_tag="IXH60_000243"
     CDS             20800..21444
                     /locus_tag="IXH60_000243"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1989214.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21459..21788
                     /locus_tag="IXH60_000244"
     CDS             21459..21788
                     /locus_tag="IXH60_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989215.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21802..22740
                     /gene="holB"
                     /locus_tag="IXH60_000245"
     CDS             21802..22740
                     /gene="holB"
                     /locus_tag="IXH60_000245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1989216.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22776..23600
                     /locus_tag="IXH60_000246"
     CDS             22776..23600
                     /locus_tag="IXH60_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1989217.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23593..23940
                     /locus_tag="IXH60_000247"
     CDS             23593..23940
                     /locus_tag="IXH60_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1989218.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            24009..24881
                     /gene="rsmI"
                     /locus_tag="IXH60_000248"
     CDS             24009..24881
                     /gene="rsmI"
                     /locus_tag="IXH60_000248"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1989219.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24990..26111
                     /gene="dinB"
                     /locus_tag="IXH60_000249"
     CDS             24990..26111
                     /gene="dinB"
                     /locus_tag="IXH60_000249"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1989220.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(26165..26767)
                     /gene="nrdG"
                     /locus_tag="IXH60_000250"
     CDS             complement(26165..26767)
                     /gene="nrdG"
                     /locus_tag="IXH60_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1989221.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(27082..29271)
                     /gene="nrdD"
                     /locus_tag="IXH60_000251"
     CDS             complement(27082..29271)
                     /gene="nrdD"
                     /locus_tag="IXH60_000251"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1989222.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29597..30364
                     /locus_tag="IXH60_000252"
     CDS             29597..30364
                     /locus_tag="IXH60_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989223.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            30354..32396
                     /locus_tag="IXH60_000253"
     CDS             30354..32396
                     /locus_tag="IXH60_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989224.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            32677..32826
                     /locus_tag="IXH60_000254"
     CDS             32677..32826
                     /locus_tag="IXH60_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1989225.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32837..34351
                     /gene="dltA"
                     /locus_tag="IXH60_000255"
     CDS             32837..34351
                     /gene="dltA"
                     /locus_tag="IXH60_000255"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1989226.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            34348..35553
                     /gene="dltB"
                     /locus_tag="IXH60_000256"
     CDS             34348..35553
                     /gene="dltB"
                     /locus_tag="IXH60_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1989227.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            35598..35831
                     /gene="dltC"
                     /locus_tag="IXH60_000257"
     CDS             35598..35831
                     /gene="dltC"
                     /locus_tag="IXH60_000257"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1989228.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35834..37102
                     /gene="dltD"
                     /locus_tag="IXH60_000258"
     CDS             35834..37102
                     /gene="dltD"
                     /locus_tag="IXH60_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1989229.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            37243..38325
                     /locus_tag="IXH60_000259"
     CDS             37243..38325
                     /locus_tag="IXH60_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1989230.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            38325..39128
                     /locus_tag="IXH60_000260"
     CDS             38325..39128
                     /locus_tag="IXH60_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1989231.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFVFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            39198..39938
                     /locus_tag="IXH60_000261"
     CDS             39198..39938
                     /locus_tag="IXH60_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1989232.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39949..40953
                     /locus_tag="IXH60_000262"
     CDS             39949..40953
                     /locus_tag="IXH60_000262"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1989233.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(40998..>42589)
                     /locus_tag="IXH60_000263"
     CDS             complement(40998..>42589)
                     /locus_tag="IXH60_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989234.1"
                     /translation="FIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNTV"
BASE COUNT        13677 a         7445 c         8850 g        12617 t
ORIGIN      
        1 cagctgattg aacaccgttt agtggagagg ttgtgaaaaa gctgccctgc aacaagtaat
       61 aagaatagcc aagcaaaaac agcttttctt gtttttgaat tggctgaact tattaccgca
      121 gatgcaagct tttgaacacc gtttaccaag aggttttgaa aaagacgttt agtttcgagt
      181 attaagaaaa attttgtgaa aatggctttc tacatttttg cataaatttg gcttaatacc
      241 gagaaactgg cttttgaata ccgtttatcc agaggttgtg agcctaccat ttaactccga
      301 atcacaagaa ataatttcaa aaacagcttc tcatgtttga tggatattgt gacttgtgac
      361 cgaggagttg gcaggagaac accgtttatt tagaaataag gaggcacgac ttttgtcgca
      421 tctccttatt ttttacttat gtgattcgtt accttcaatg aatccctaca aggcactttt
      481 gttgaaaaaa aggtcatctt tgaaaaaatt agtggaattg ttttgttttt gatattattt
      541 aatagaaaag atatgccaaa aatccgagaa ctgtggacta cgttttttac gtaaaggagg
      601 taaataaggg tgaaagaaaa agattgtttc tacgataagc tatataaact gttgctgcaa
      661 gtaggaaaaa gtagttcttt gttaggtggg atattatcta tcataatgtt tgttttattt
      721 ttttatctat tatttcactt aaaatttttc agtaacgatt ttctgatttg cttaggttta
      781 acgttagcta tgtttatatc aagcttatta tgtgggttaa attttttttg cactcataaa
      841 ggacttaaga aataatttct caaaggatat cgaaaaagaa aaaattgtaa gcggatagta
      901 ttcatttgag tacttgactt tcccttagtc agcttgtatt atacaccttg ggcacccttt
      961 tagagggtca gaagtttaca gaaatttcgg ctccctattc ttattgaaga atagggagct
     1021 ttgtttattg tctgagactt ttcctgcaca taaattaaaa aggagttttt gaacttatga
     1081 caaaatatat ttttgtgacc ggcggtgtcg tttcatcaat cggaaaaggg attgttgcag
     1141 cttctttagg acgcttattg aaaaaccgcg gcttaaaagt aactatccaa aaatttgatc
     1201 catacatcaa cgtggatccg gggacgatga gcccatatca gcacggagaa gtattcgtaa
     1261 cagatgatgg tgctgaaaca gatttggatc tgggccacta cgaacgtttt attgacatca
     1321 atttgaacaa atactccaac gtgacgacag gaaaaatcta ttctgaagtt ttgcgtaaag
     1381 aacgtaaggg cgaatacttg ggagcaactg ttcaagttat tcctcatatt acaaatgaga
     1441 tcaaagaaaa aatcatgcga gcagctaaaa tgactgattc agatgtgatc attacagaag
     1501 tagggggaac agtaggggat atagaatctt tgccattcct tgaagcactt cgtcaaatga
     1561 aagcagatgt cggtacagat aatgttatgt atatccatac aacattgatt ccttacttga
     1621 aagctgctgg agaaatgaaa actaaaccaa cgcagcacag tgtaaaagag ctacgtggtt
     1681 taggtatcca accgaatatt ttggtggtac gtacagagca acctgtttct caaagcgtga
     1741 aaaacaaatt ggcacaattt tgtgatgtcg aaccagaagc agttatcgag tcgccagatg
     1801 tagatacact ttactccatc ccattagttt tacaagcaca aggaatggat caaattgttt
     1861 gcgaccattt aaaactagat acaccagaag cagacatgac agaatggaaa gaattagaag
     1921 aacgtgtctt gaacttgaag aaaaaagtac ggattgcgct tgtcggtaaa tatgtcgagt
     1981 taccagatgc atatatctca gtagttgaag cattgaaaca ttctggtttc gcctataatt
     2041 cagacatcga gatcgattgg atcaaagcac aagaattaac gagagaaaat gtagaagaac
     2101 gtttgaaaga cgcagatgga atcttggttc ctggtggatt tggtgatcgc ggagtagaag
     2161 ggaaaatcga agcgattcgt tatgctcgcg aaaatgacgt tccgttttta ggtatttgtc
     2221 ttggtatgca gatggcttgt gtcgaatttg cacgtaacgt cgttggatta gaagatgcgt
     2281 catcagctga aacgaatcca gatacagcaa acaatattat tgatttaatg gctgatcagg
     2341 aaaacatcga gaatttgggt ggaactttac gccttggatt atacccatgt aaaatcaaaa
     2401 aaggaacgaa gacggctgaa gcatatgatg gagccgatgt tgtgcaagaa cgtcaccgtc
     2461 accgctatga gttcaataac aaatatcgtc aattgtttga agaacaaggg ctagtctttt
     2521 caggtgtctc accagataac cgtttagtag aaatcgtaga gctgccagag aaaaaattct
     2581 ttgtgggatg ccaattccac ccagaattga tttctcgtcc aaatcgcccg caaaaattaa
     2641 tcaaagcatt tgtcggagca tcattagcag atcacgaaac aaaataattt ttgaacttga
     2701 tagaaagaga aaagtagcaa gagaagggga taagcaattt tcttgtcact tttctctttt
     2761 tctgatttgt tattatttgt aggaaaaaag atagaaagca aaagactttt ttcgattaaa
     2821 gcgctattgg atattttatt ttttgcaaaa aacttcttga aaattttcat tttcattgat
     2881 tttagtagaa aactaccgaa atgtttggta aaatagggtc gttggttaag aattgtctta
     2941 acaaatttag ttttacaaaa cttaggagga attttattat gccagtagta tcaggagctg
     3001 aatttttaaa agctgctcgt aaaggcggtt acgctgtcgg cggattcaac acaaacaact
     3061 tggaatggac tcaagcaatc cttgaagcgg ctgaagcaaa acaagcacca gtacttatcc
     3121 aaacatcaat gggtgcagct aaatacatgg gtggttacaa aatctgtaaa gatttaatca
     3181 ctgatttagt tgaagcaatg aacatcactg ttccagttgc tatccactta gaccatggtg
     3241 attacgaagc tgctttggaa tgtatcgaat taggctatac ttcagtaatg ttcgatggtt
     3301 cacatctacc atttgaagaa aacttgaaat tagctaaaga cgttgttgaa aaagcacatg
     3361 caaaaggtat ctcagttgaa tgtgaagttg gttcaatcgg cggagaagaa gacggaatca
     3421 tcggtaacgg cgaattagct gatattgaag aatgcaaaca aatggttgct acaggtatcg
     3481 actacttagc ttgcggtatc ggtaacatcc atggtcaata tccagaaaac tggaaaggat
     3541 tagcttttga ccacttacaa gcaattgctg atgctgtagg tccagacatg cctttagtat
     3601 tacatggcgg atcaggtatt cctcaagatc aaatcgaaaa agctatctca atgggtatct
     3661 caaaagtaaa tgttaacact gaattccaat tatcatttgc tgcagctact cgtaaatata
     3721 tcgaagctgg taaagataaa gaaggtaaag gattcgaccc tcgtaaatta ttagctccag
     3781 gtaaagcagc aatcgttgaa gatgctaaat ctcatatcga ctggttcggt tcagctaaca
     3841 aagcgtaagc atccacataa cagatacgat atataggaat ggagaacgac ttttgaagtc
     3901 gctctccatt cttttttact taaaacggca gaccatgctc gtttcttttt tttatctatg
     3961 ataaaataca agcatatcca tgaaaaaaga ttaataaaca aggtggaaac agatgaaaaa
     4021 aatcgtaatc aaaggaaacc gacctttagc tggtgaagta acgatcagcg gtgcaaaaaa
     4081 tagtgcagtc gcattgattc cagctgcaat cttagctgat tccccagtga tcttggaagg
     4141 tgtgccggac atacaagatg ttcattcatt aattgaaatt ttagagatca tgggcgcaac
     4201 tgtccatttt tccaacaaca tactagaaat cgatcctcga gaagtcgtat cgattccgat
     4261 gccgcacgga aaaatcaata gtttgcgtgc ttcttattat tttatgggaa cactgcttgg
     4321 taaatttggc gaagcggtag taggcctgcc aggtggctgt tatttaggcc ctagaccgat
     4381 tgacctacac gtcaaaggat ttgaagctct aggtgctgaa gtgacaaatg aacatggtgc
     4441 catgtattta cggacagaaa ataaaggatt gcgtggcaat cggattttca tggacgtggt
     4501 ttcagtcgga gcaacgatca atgtgatgtt agctgctgta aaagcaaaag gacagacagt
     4561 aattgaaaac gctgcacgag aaccggaaat catagatgtg gcaactttac tgaataatat
     4621 gggggcaaaa gttcgaggag cgggtacaga tgtgatccgt atcgaaggag tcgaaacatt
     4681 acatggctgt cggcatttca tgattcctga ccgcatcgaa gcaggaacct atctagcatt
     4741 agcagctgca gtaggaaatg gtatcaaagt gaaaaatgtg atctttgaac atttggaaag
     4801 ctttatagca aaattgcagg aaattggtgt ccatatgaaa atcagcgaag atgaaatcga
     4861 agtttacccc tcgaaagaat taaaacccgc aaacatcatg acctatcctt atccaggatt
     4921 tgccacagat ctgcagcagc cacttactgc gttactattg atgactacag gcacttcaga
     4981 aatcatcgat acgatctatg cgaaacgtgt gaaccatgtg ccggaactag cacgaatggg
     5041 cgctgatatt accgtggaag gaaatatgat catcgtgaat ggtccaaata aacttcacgg
     5101 cacagaagtc gtggcttctg atcttcgggc aggtgcttgt ttagtgattg ctggactatt
     5161 ggcagaaggg acaacgacga tttacaatgt agattatatc ttacgcggtt atgatcatat
     5221 tatcgagaaa ctaacagcct taggggcatc aattgaaatg atagaggaag tgggagctga
     5281 atgagcgatt atttgacaat ggcagaacta gagaacaaaa cgttaaaaga agtttatagt
     5341 tatgccaaag aatttaaaat tccttattac agtaagatga ataaaaagga attatctcta
     5401 gctgtgattc gtgcacaagc agagaaacaa ggattttatt atatggaagg aatattggat
     5461 atcgttggtc aagatggtta cggtttttta cggccaatca attatggacc aagtgcagaa
     5521 gatatctata tttctgcctc acaaatcaga cgattcagtt tgagaaatgg ggacaaagtg
     5581 gcaggaaaag cccgtccacc taaagaatcg gaacgttatt atggtttgat gcacgtggaa
     5641 agcgttaatg gaaaagatcc ggaagaagca aaagaacgtc cgcattttcc agccttaaca
     5701 cctctttatc cgcaaagaca gcttcaactg gaaacaacac cgcaaagact atctacccga
     5761 atgatcgata tcttttctcc aatcggtttt ggtcagcgtg gattgatcgt cgcaccgcca
     5821 aaagcaggga aaacttctgt attaaaagaa atcgcaaatg gtatcaccga aaactatccg
     5881 gaagtagaat taatcgttct actgattgat gaacgaccgg aagaagtaac ggatctagaa
     5941 cgaagcgtga aaggcgacgt ggtatcttct acatttgatc tgcagccgca aaaccatact
     6001 cgagtttctg aattagtatt ggaacgcgca atgcgtttag tcgaagataa acgagatgtc
     6061 gttatcttga tggatagtat tactagactt gctcgggcct ataacttagt cgttccacca
     6121 agcggacgta cattgagtgg cgggattgac ccagccgcat tgtataaacc taagaaattt
     6181 ttcggggcag caagaaacat cgaagaaggt ggcagtttga cgattttagc gaccgcttta
     6241 gtcgatacag gaagccgtat ggatgacgtg atctatgaag aattcaaagg aactggaaat
     6301 ctagaattac aactttcacg tgaattatct gaacgtcgga tcttcccagc catcgatatc
     6361 aaaaaatcag gtacccgaaa agaagaatta ttgatgccaa gtgaagaatt agaagaaatt
     6421 tggaaacttc gaaaaaatat gactggagat tcattagaat atacggaaca actgatccgc
     6481 tttttgcgta aaacaaaaaa caatcagcaa tttttcaaag aatttcaaga tgtctccttt
     6541 ggtaaaaaaa atccgaccag aaatctgaaa agataattgc aaccttgact gaaacatgtt
     6601 aagatgttaa tgttgtaaat cgacaaatca actctggttc agcaaatgga gcagggcaaa
     6661 ggagcgaaaa aatcatgaaa gaaaacatcc atccagatta ccacccagta gtattcatgg
     6721 attcaacaac tggtttcaaa ttcttgtcag gttcaacaaa aacttcacaa gaaacagttg
     6781 aatgggaaga cggtaataca tacccagtca tccgtgtcga agtaacttct gactcacatc
     6841 cattctacac tggacgtcaa aaattcacac aagcagacgg acgtgtggac cgtttcaaca
     6901 aaaaatacgg tctcaaagac gcaaacgctg cggaataata aaaacgctga taactcaacg
     6961 tttagcagac tatctgatag ggatagtctg tttttttatg ttttttgcca atttaaaact
     7021 tcgtttgatg ctgaaaaagt cgtaggatca taatgcacaa aacaatgaaa aactagtata
     7081 tagttttaat tatccacttg ataaaaacac cttatattaa cgaaatgaag aggggttatt
     7141 tcgaagtaaa aaaccattta cctaaaatta gaagctaagc tttcctttca aggaaggctt
     7201 tttgttttgt tatagacttg cgattgtttg tgagctagat cgttcatttt aatgacttat
     7261 acacgcggat gatgggtctt tttcctctgt tcattcataa tattgaacgg tacagctagc
     7321 gatgctatac tgacctcagt tccaagaaaa cgttaacatt ttttgtgggg gagaggtttt
     7381 ttatgaggtt tgatcaattg atcgcagaag aactgattgt tttcgatcca tcgatagatt
     7441 caaaaggatc attatttgag cgaatagggg atgtgttgaa aaaaacaaat aaggtaagaa
     7501 atgttcaaaa agtcgttaag gcactattca aacgagagga ggagatctcc acaggaatag
     7561 aagatggatt tgggattccc catgcaaaaa gcaaatatgt gacaaaacca gcactgtgtt
     7621 tttttcatac agccaaatta acggactata gcgggataga tgaaatgcca attgaatata
     7681 tttttgccat cgtagttcct gaaaaatcag cagatatgca tctagagatt ctcagcacac
     7741 tctccagacg attgatgaat gcagatttta gaaagggaat aaaagaagcc actacgccag
     7801 cagatatcat cagacagatt agtcaagaat gaaaatagta gagtctttgt ccgagtatcg
     7861 atagtgaaga aaaaggagga aaataatcat gaaaatcgtt ggtgtaactg cttgcccgac
     7921 tggaattgct catacttata tgtcggcaga aaaattagaa gtaacagcaa gaaatctaaa
     7981 ccatcaagca aaattcgaaa cacaaggtgt aaaaacagaa aatcagttat ctgaacaaga
     8041 aatcaaagag gcagatgcaa tcattcttgc agtagataaa gaaatcgaat tggaccgatt
     8101 tgccgggaaa aaagtaaagc gtgtctctac aagccgagca atcaaagaac cgcaagtagt
     8161 aatagacgaa gcattgagag atattggcgt attcgttgtg tctaatgaaa aagaaccagc
     8221 tgcaaatgag aaaaagccga caatctataa tcatttcatg aatggtgtga attacatgct
     8281 tccatttgtg atcgcaggcg gaatcatcat cgcactgagt tttgcctttg ggattacagc
     8341 ggctgatccc aactcagcag actataatgt gttagcagct gcattttcaa ggatcggagg
     8401 agatacagcg tttgccatga tggttcctgc gttaggcgcg gggattgcta catcgattgc
     8461 tggaaaagca ggttttgccc caggaattgt tgccggatta ctggcctcta caggtggttc
     8521 aggttttctt ggcggaatga ttggtggcgt gctagcaggg tatatctgtg attttctggc
     8581 aaataaaatt agcgtaaaaa aagaattttc tgctatttat cagctgattg tcgtcccctt
     8641 tttatcgatc ttaattattg ggcttttgat ggtatttgtg attgaacaac cgatcacatg
     8701 ggtgttggat gctttgacaa attggctgaa tagcttggga aatacatccg ggctattgtt
     8761 cggattactt atcgggatca tgatggcagc tgatatgggt gggccgatca ataaatcgac
     8821 ttcgactttc tcgatcggtt tgatgtcagc tggagtaaat gcaccgattg ctgcttgtat
     8881 ggctgcagga atgacacctc ccctaggtat tgctttagcc actttattat tcaaacataa
     8941 atttaccaaa gaagaacgga cttcaggtca atcatgctgg attttaggcg cttcgtatat
     9001 tacagaaggt gctataccgt ttgcggtatc tgacccgatc cgcgtgatac ctagtttgat
     9061 gacaggttca gcagtcgctg cagggatttc catggcagta ggagttactt caatggcacc
     9121 tcatggaggg atttgggtga tgctaattcc aaatgttatg aacaatttac ctatgtatgt
     9181 cttggcaatt gcatcaggta cagtcactac agcctttatg cttggactat tgaaaaaaac
     9241 agtgaaagag aaaaaacaag acaagtggac gaaaaatgaa aatctaacaa atgaggtgga
     9301 agcgtaatgt tatatacaat gaaagagtta ttgaaagtgg ctaaagagaa tcaatttgca
     9361 gtaccagcct tcaatatttg cagctatgat atgatgaaag caatcatcga agaagcagag
     9421 agattagaag caccagtaat cttagaaatt catcctgatg aaatcgccta tttaagtgat
     9481 gagtttgttg cggcagtaag agcctatgct tatacaagta aactgccgat cgttatccac
     9541 ttggatcatg gcggtacgat agaagatgtc ttacgggcga ttcgaaacgg ctattcttcc
     9601 gtgatgatcg atgcgtcttt gaaatcattc gacgacaatg tacagattac gaaaaaagtg
     9661 gttgcgttag ctcatgcggt aaatgtctca gtggaggctg agcttggaac gattgggaat
     9721 aatggttctg ctgaaggagg tgctgaaaca atcatttata cagatcctga tcaagcgaag
     9781 atttttgtga aagaaacagg aatcgataca cttgctgtag caattggaac agcgcatggc
     9841 ttgtatccga aaaacagaac tccaaaatta aatatcgact tactgaaaga attgaaccaa
     9901 caaatcgatc ttccatttgt tttacatggc ggttcaggta atccggacgc agaagtcagc
     9961 gaagcagtaa aatacggagt tgccaaagtg aatttgagct cagatcttaa aagcgtcttt
    10021 ttcaatgctt tgagagaaat attgagtgaa aatccatcga tgtacgagcc gaatgcaatt
    10081 tatccagcag ctaataaaaa ggtacaagaa gtggtcaaac ataaaatgga aattttgaac
    10141 acagtaggca aggctagttt atactaaaaa taaaaatgtg tggtcaagag ggtatgacaa
    10201 tagtcttgtc ataccctctt aaccacacac gatgttcaga agtagttttt tcgaaaaatt
    10261 tcttcttatt tctcgaagcc aatcacttct gtcacaacct cttttttcct gttactatat
    10321 aaagtaaatg gggtgatttt atgttgaatg aagtggagaa tagacagcag gagattgtga
    10381 attatctaaa aaacaaccaa tttgcaagag tcaccgattt gatcgagtta gtaaattata
    10441 gcgaagccac gattaaacgt gaccttgttt ttctggaaaa aagtggattg atccgtagag
    10501 ttcgcggtgg cgccatgctt attgacaatc gaaaaattga cgtgccttac ctgatgaaga
    10561 tcaccaaatt agaagaagaa acagaaaaac gtaagattgc agatacagct tcttctttga
    10621 ttcgagatga tatggtctta ttcttggatt ccagtacaac ctctttacac ttagtcagaa
    10681 acctagcaag atttgatggt ttgcagatca ttacgaatgg catcgtcaca gctgctatgt
    10741 tgagtgaata tacaacggca aaagtatctg tattgggagg ggcaattgtt ccgaaacgag
    10801 caacaatcaa cggttcaaaa gcctataacg atgcattgtc atattatgcg gatttagcga
    10861 taatgagttg tcgcgggcta gatttcagcc aaggagtaac agaaactcat gaaggagagg
    10921 caatggtcaa aaaaagcttt tagaaaacaa tcagaccggc ttgccgtttt agttacacag
    10981 caaaaaattg cacataaatt catgcatcaa gccattgctt ttcatgaaat cgattatctg
    11041 gtgacaagta aagaactgac aaaagaacag tatgcgaaag taaaggaaca ccatatcgaa
    11101 tgtctgtacg gataaattat tacgtgaatg ttctaagtgg cttcctcatt tttagatagg
    11161 accttatctt tgctcgcttt tctcagccga tggtataata atgaaaaggt tatcttgtta
    11221 gataatctta cttttttatc caggagaaag gagtgcagaa aatgaaacat gtgaaggcgt
    11281 tggtagtgaa ggcaatcatg atctgggcga ttctgtgggt cgtgctgaca ggtttatacg
    11341 gtgttagctt tatggattca acgattgttg gggtaattat tgtagtcatg atctatgtgc
    11401 taggggatct gttgatctta cgtaaagtag ggaacatcgc agcgacgatc gcagatgctg
    11461 gttcagctgc agttatcttg tggctctatc tatattttat gaatgataca gtcgatattt
    11521 ggatgaaagt attgattcct gctctattga tcggtgtggc agaatggttc ttccacaagt
    11581 ggttgctaag tcaaggcatc gtaccagatg aacggaaaat ggaataaatc aacagttaaa
    11641 ctattcttat cgataaatga aagctttaaa aataaataga tgtttaatta agaataatca
    11701 taaactattt ttactcaaaa aaccgctcag attattttca gatctgaacg gtttttttta
    11761 tctattcatc aagatcaaat ttcatttttc aatgattcaa tcaattcgcc ctctgcttca
    11821 tgactatgtt ttcggccatt aacaagatag agtgctgtac atatcaatgg aatgactccg
    11881 gcaatcacat acaatcctct aaatgaagta agagatttta gcatccccat caaataagga
    11941 ccaacgccta agcctaggtc taaaccaata aagtaggtag ataaagcaat actgattcga
    12001 tgttcgcgga cgatttttaa acaaacagcc tgtccatttg acataaacgt accataacct
    12061 aggccgacta atgctccaga gataagcaat gtcacactat tcgtcgttac actcagtaaa
    12121 aataatcctg ctgttaagaa taaatagcta ggatacatca cgtattgttc tcctttgaca
    12181 tcgaaaatcc ttccagtcaa tggacgtgta aatgtgatga ccaaagcata tacgacgaag
    12241 aaaaatgacc cagcagcaac taagtggatt acttttgtat aagaagataa aaatgataaa
    12301 acacttgaat aagaaagacc catcaaaaag gcaattcctg taatgaaaag tgctttcttt
    12361 tcaataaaac tattgatatt ccatgagcga agagatattt tttgttcttc agataatgga
    12421 atatttttta caggaaagac aaggcaggca atcgtaacaa agaagaccag tacaattgaa
    12481 aaccagataa tgaaacgaaa atcagtcata ttcagcaaaa tcattcctaa aaatggccca
    12541 acagcggcag ctaaactagt acttaatcca taataattga tgccttcccc ttttttagat
    12601 gcagggatat atgcagtaac gatagcattt gtagctgtag aaactgtacc gtagccgaat
    12661 ccattcaaga atcgaactaa atacaatgcc ccgatagttg gcgtataaag atatgctaat
    12721 gttgaaagga gatagaagat cgcgccgcct cttaaggtaa acttccgacc aaatagttct
    12781 aactgtttcc ccatgaataa gcgtgccagc aatgttccga tgatatagat cccagaagca
    12841 aaacctgctt ggctcaagct ggcgtgtaag gtgtcttgcg caatgactgc catgatgacc
    12901 atcaacagat aataaatcag gtagacaata aaattgatca atgtgattcc aataaaatat
    12961 ttattaaata atttcttttc catgtatgtt atacattccc cttaagctga atatgctttt
    13021 aatttttgta tttgaacttc tacatcgtga taattggatg gatagcgatc aacaagttgg
    13081 cgaataaata aatctcttgt agacataaac agatttaaaa attcttcgca ttcgtttgtt
    13141 ggattctcat ccactgcttt gatcaattcg atcgagtttt gatacgcatc atttaaaaag
    13201 actaaagaag ctttcatatc aaatcttgtt tgatatgctg tttctaatcg attaatgatt
    13261 gtgttcatcc agtaattcat gttttttccc tctattctta aaaaatttta cttctgcagt
    13321 ataacgaata aaaaaaagga aaaaaatcgc ataagtgatg gaaaaaggta cttaacaaat
    13381 gttaatatca ttagtaaaat acctttggac tttaaaaagt tttaaagtac tgtatgatct
    13441 ttttagatga aagtttggat gggggagcat cattggatag tagagtctta aaggaatatt
    13501 tggataaaaa tgattttccg attatacgca aaggatatca taaatatttg acattcgaag
    13561 ggatggaaga ttcccatacg tatatcttga aagagggaac ggtaaaagca agtgttattt
    13621 caaatgatgg gagagaattt aaccttcgct atatcaaaga tcttgaaatc gtctctcttt
    13681 taaaagacga atattcacaa ttcatcgatt cgccgtataa tattcgagtt gaatcagaat
    13741 atgcagagtt ttatcgaatc aatcgtgtgc gcttttggca aaatatcaac caagacccaa
    13801 agctacaaca ctatgtaaaa gagtattacc gctatcgtct gctttattcg atgaaaaaaa
    13861 tgcagcaaat gctattaaac ggaaagctgg gagctgtttg tacgcaactt tacgaattat
    13921 atgatttatt cggtgtgcgg acagaagaag ggatgctgat cgatttttcg atcacgaatg
    13981 aagaaatcgc caaattttgt ggcatctcaa ctgctagcag tgtaaacaga attttaaaac
    14041 aactgaaaaa agatggtatt atcaccgtta aagaccatca tatcgttatt ttgaatatgg
    14101 acttgttaga agacaatatt atcttttgat gaacaaagag gttgtgaaat cagcgttttg
    14161 acctgagtat tagagaaaca aacggaaaaa tagttcctca tattttgccg ttttttgagc
    14221 taataccgag ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta
    14281 actctgagtc acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga
    14341 cttgtgaccg aggagttggt aggagaacac cgtttaccaa gaggttgtga aaaagctgtt
    14401 ctgcatcaag taataagaag agccaagtaa aaacagtctc ttatgttttt aacttggctg
    14461 gacttatcac cgtagatgta agcttttgaa cgccgtttat ttgcaacaaa aggggctgtg
    14521 acacgatttt tgtcacagcc ctttttttgc gaacattcat ttagcaattt ttagaaactt
    14581 ctgccgccac cgccaaaagt cccgccaccg ctagaatggg tagtacttcc gccaccgccg
    14641 cttccaggag gtgggctttt ggggattctt cttgtcgtga caaaagaatt tgtcagacga
    14701 tcggtttttt cagttaattt gatactggat ttttctcgga atggatattt gtagatgcct
    14761 gatttcaatt gataacgcga aacagtcaca aaataaaaga caagactaag gataagagcc
    14821 aaaacaacag caatgatgat ttctactgtc gtcaaaacct tatagcgtgt aattttgcca
    14881 gtttcttcgt ctacacgata atgtcctcct ggaactccgt cttccacata tgttgctgat
    14941 ttagacaggt aagcttttgc agcattgtaa taggcagcat ttgtcatctg atcataaaca
    15001 tcatctaaga tcgaatcgat tctgctatca gtcaaataat cgatcatatt accagaagtc
    15061 gaaatgtaga tttcacgctg tcccatatct aatagcaata ctgagccatt ttgatcattt
    15121 ccgactgcgt ctcttaagta attatcagca aagtctcttg gttcttctga attagatgta
    15181 gtcgttacga tcaagactcg tcctttgatt ttttcattca gcttttctgc ctgttgttcc
    15241 aaagattgga tttgatctgt tgtgaataac ccagcctcat catttaccgt agctgtatct
    15301 gcatgagtag ccaccggcca gccaacactt actagcaaaa gaaagaataa aaaaaatttt
    15361 ttcatcatat caagtaacct ccgatcagaa agaggatcgc tagcccagcg aaaatcccaa
    15421 gagcggtcag tcccaatttt ttctggctga tcggcaggac accgctgacc tttcctgttt
    15481 gtccattcat tgcgtagtag tagacttttt tacttgagtc attgcttcga taagtaacaa
    15541 gccataccgg cagtaatacg taattattta cttcgtttgt gattgaagca gaagttcgca
    15601 caccggtcaa tgttgtatat ccatttgcgg tatcttttaa cagcttctct gaatagtctt
    15661 ttaattcaga ttgaatctgt gatttgattg cctcatattc gatatctctt ttttctgctt
    15721 ggaacccggc taaatactgg cttttaaaat caattgcttt atctaacggg aaaggttgca
    15781 cgccttcgac catcttttgt tgcgtatttt ttgataaggc attttttact agttctttaa
    15841 aagaaagctt gcctgctctt gaaacagcaa attgcttcgt ttctgtgtat tcgatgtcac
    15901 ctatccgcca tatgcggata actgtaccat tcgcctgcat ggcaccatct acttcagcat
    15961 ctgtagccca ataagggaaa tacacacctg tcaacttatc gatttgatcc ttattgaaaa
    16021 aagctttggg aacaaaccat tttttcttgg tccaagcgag aaatctgttg attgcttctt
    16081 ctttctcgac agcaaaagga agtactttat ttggcaagaa ttctccgctt aaacgtcccg
    16141 atagtacgac gggattatgg cagtagtaac aataagtcgc agcagttgtc gcatcagtca
    16201 cgatctctgc accacagttg ggacaaagaa aaagatctat cgctgcggta ttatctgcag
    16261 attcgtccgc tgtatctgaa aaaccgcctg cctcgtcaac cgctttgcgc tcggcttcat
    16321 tcatatcggc aagctgttct tgggctgtaa aagtaaaatc ttcggtaggt gaaggttctt
    16381 ctttgctttc ttcgccagcc atacgcgctt ctttttgctt ttgctcatat tggctgactt
    16441 cttcttctgt atagatattc aggcagtatt cgcaatgaaa tttttggtct ttgggatcaa
    16501 aagtcaacgg accgccgcaa ttaggacatt tatgtgtaag aacatccatc agctgcacac
    16561 ctttctaaat aaattaatta acggggattc ctttaaacgg tttaccacag tttggacaga
    16621 atttagggat gccgttggaa agatccacga tctcattgca ttcactgcat cgcatttgta
    16681 gcttaggctg aacgttttct acaggtttgg cagctccgca gttggaacag aattttccgg
    16741 aattttctgt cccgcaaact ggacatgtcc aagtatccgc atttccttgt gcagtttgtt
    16801 gattttgttt ttctgcttgt tgtttgattt gttcttggtt attttgagct gcttgagaaa
    16861 gataagaacc atttgcattc attcccatac ctacacccat aaagccggtc attgctcctg
    16921 cttcattttt tccggctgct tccataccac gtgcaatgga accttgcacg tatccttcac
    16981 gtacgctagg atcacctagc atcgcccctt cgttacgcat attgatcagt ttcacagaat
    17041 catcggtgta agaaatgctg gctacagcca cagaaacaat ctccatccca cgtaattcac
    17101 gccaagaatc atccaatgca gtatccatgt atttgcttaa ttccaaactc ttagatggta
    17161 cataagaaat ccgttgacca tctgcagaca tctgattgat tgctgattgt agcgctgtta
    17221 ggaattcagc taaatactgc tcattgatat cattgatttc cacttgtgtt ttgtttttag
    17281 ggattgcgtt tgtataaaat aaaatcgggt caacgacatg aatagaataa gtgccatgtg
    17341 cccgtaagaa taattctgcg ttatagaaat tgtcaaagta gttcaacggc gcgcttgtac
    17401 cgaatttgat tccctttatt tcttggagat tgatataaaa aacttgttgt ttctgcggtg
    17461 tgacgccgcc aaatttgaat cggtcaaaag tttcagcaat tgcttctttc agtgttccat
    17521 tgaacatgga aggagctgcg tcatttttaa tggtgtaata accttcctca gcggtataat
    17581 cgatgatttt tccaccatcg accaaaagca tcatcatgtt tggatagaca tggacgactg
    17641 ttccgtctgt taaaacatct tctgttcctt tacgattgga gccacgtttg tcatctttgc
    17701 ggactttgac accttttgtc atgaccgttg tatcactcat attgtctggt tcgatgactt
    17761 ctagccattg atctgccaga ccgccgccaa ccatacttgt tgctgctttg ataagaccca
    17821 taaaaaattc ctcctctgat tcagctagtg ccaaacaatt attgtttatt tattatacca
    17881 taggaagatg tgaaacttct cggactagag acggaattga aatggaaaat tgcgtattgt
    17941 ttaaatacct gaaaaagccg agaaggatag tagttttttt acctaatatc acgtataata
    18001 agagatagtg aatttttaaa aaaaggggga tccttatggc atatcaagca ctttatcgag
    18061 tctggcgttc gcagcgtttc gaagacatcg ttggacaaaa agctgtgaca cagacactaa
    18121 aaaatgctat tgtttcccac aaaacctcac atgcctactt gtttacagga cccagaggaa
    18181 ccggaaagac gagtgcagca aagattttcg caaaagcaat caactgtccg aacagtaaag
    18241 acggtgagcc atgcaacgaa tgcgaaatgt gtcgatcaat tactgctggt acgcaagaag
    18301 atgtgatcga aatcgatgct gccagcaata acggagtcga agaaatccga tttatccgtg
    18361 atcgggcaaa ttatgcgcct acaaaggcag cgtacaaagt gtacattatt gatgaggtac
    18421 atatgctttc aacaggagct ttcaatgcct tgttgaaaac attggaagag ccaaaagaaa
    18481 gtgtcatatt catcttagca acgaccgaac ctcacaaaat accagctacg atcatttcac
    18541 ggactcagcg atttgatttt aaacggatca atacggcaga tattgtcgaa catcttgaat
    18601 ttatcttgaa agaatcgact atcagctatg aaccagctgc attgaatgtg attgcccgtt
    18661 ctgcagaagg cgggatgcgg gatgcattga gtattcttga tcaggcaatc tcgtttagtg
    18721 aaggaacaat tactttagat gatgccatgc aagtgacagg tagcttaact gacgaaatga
    18781 tggacagtta cttgagtgct tgcgtgaaaa aagaagtacc agaagcattg gaaacattga
    18841 accaaattct cgcagccggc aaagaatcac gtcgctttct agaggatatg ttacaatact
    18901 gccgtgactt gctgatgtat cagcaagcac ctaacctagt tgaggaacaa tcgagtacga
    18961 tcagaagtac atttacagaa ttggcacaaa ctgtttcagc ggatcagctg ttttcaatga
    19021 taaagatttt gagcgatact caaaatgaaa ttcgttttac caatagtgcc gctatttatt
    19081 tggaagtcgc tacagtcaaa cttgcacagc taccgtctgc ttctgttcct tcagcagatg
    19141 tagcatttgc agatgatcag atagtaaaac agctacaaca agagattaag gaactaagaa
    19201 acgaaatcaa ggaactacgc agtaatacag gaattgagaa tacaaaagta cagccggtac
    19261 agcaaacaaa tcataaaaaa cagacaagta cctatcgtgt accgacggaa cgagtatata
    19321 aagttctgcg ggaagcgaca agacagcatc tgaaccaagt aaaagaagta tgggaagact
    19381 tgctgatgag tctatctgtc actcagcgag caatgcttaa agcaagcgaa ccagttgcag
    19441 ctggacctac aggactagtg attgcgtttg attatgagat tgtttgtcaa agagcagcaa
    19501 atgacgaaga attgcagctt tcggtacaca acaacttaag ccgaatgatc aaagattatg
    19561 cgcctgatcc tgtttatatt acaagagaaa gctggccaga acttcgaaga aattatttga
    19621 cgcaggcaca agaagaggga acagaaaact cttttgcttc tgaagactca gaaggagaaa
    19681 tcgagttgct tcctcaagaa caagaagtac cagaagaaac acttgtagta aaagcagagg
    19741 aactgtttgg ctccgaagct gttacagtgg tagatgatta ataaaaagga gtagataatt
    19801 atgatgcgtg gaatgggcaa tatgcaaggc atgatgaaac aagtacaaaa aatgcaaaaa
    19861 gaaatggcag aagcacaaga gctgctaaat aaaacagagt ttacaggtaa agccaccaat
    19921 gaattggtca aagcggtttt tacaggcgat cgtcgcatga aagatatcca aatcaatgaa
    19981 gaagtcgttg atcctgatga tatcgagatg cttcaggatc ttgtcgtaat ggcagtcaat
    20041 gatgcgatcg cacaaattga tgtggaaact gagaaaaaat tagggaaata cgcaaaaggt
    20101 ttgccaggat tttagtttta aactgaagga gtaatgaata tgcaatatcc agaaccaatt
    20161 gcgaaactga tcgacagcta catgaaactt ccaggaatcg gtcagaaaac agcgacaaga
    20221 ctcgcctttt atacgatcga tatgaaagat gaagtagtaa atgaatttgc taaatctttg
    20281 ttaagtgtaa aacgcgattt gcatttttgt agtatctgcg gaaacatcac agaagaagat
    20341 ccctgcgaaa tatgtagaaa tccttcacgt gatcgaagcg ttatcctagt ggtcgaagag
    20401 ccgaaagatg taatggcaat ggaaaaaatg cgagaatatc acggacttta tcatgttctc
    20461 catggtgtgt tatcaccgat ggaagggaca ggtccagaag acatcaatat ttcttctctg
    20521 ttgcagcggc tgcatgatgc agatgtaaaa gaagtgatta tcgcaacaaa tgcaacaaca
    20581 gagggagaag caacagctat gtatctctca cgtctgatca aacccgcagg tatcaaagtg
    20641 actcgtctag cacatggtct gtctgtaggc tctgatatcg agtatgcaga tgaagttact
    20701 ttattgaaag ctgtcgaagg aagacgtgag ttataaattt ctaattaagt gttttttcag
    20761 atacaatata aaagcagcac aagagatggg ggcaacaatg tgaacggact atttattaca
    20821 atcgaggggc ctgatggtgc aggaaaaacc agcatcttga atgaacttta ccctcttttg
    20881 aaagaagtag caaaaacaga aatcattcaa acaagagaac caggaggcat tccgattgct
    20941 gaagagatcc gagcagtgat ccttgatcca aaaaatgatc ggatggatga acgtacggaa
    21001 gcactgcttt atgcagcagc aagaagacaa catctagtcg aaaaggttct gcctgctctg
    21061 gcaaaaggga aaatcgtttt atgcgataga tttgtcgaca gttctcttgc ttatcaaggt
    21121 gcaggccgac gcatcggcgt taccgagatt gctcggttga atgagtttgc tacagaaggt
    21181 actacgccgg attttaccct ttatcttgac gtagattcag acaccggatt aagaagaatc
    21241 aagaaaaaca gacaaaatca aatcgaccgt ttagattcgg aaggtttgga attccaccag
    21301 cgtgtacgtc atgcttactt aaagctggca gaagagaatc cggaaagaat ccacaaagtt
    21361 gatgcaagaa aaagctttga agaagtgctg caaacaagtt atcatacaat tattgaacag
    21421 tatccgcaat tttttgaaaa ctaggaaggt gaacgtttat gaaaattatt ttagcgatta
    21481 tccaagacaa agacagcaac cgtttatcaa atgaattgat tgatgcaaat atccgtgcca
    21541 caaaactatc atcgacaggc ggcttcctta aggctggaaa cagtacgttt atcgtgggta
    21601 ttgaagatga ccgtgtagaa gaagcattgg aaatcatcaa aaaaacgtgt gaatcaagaa
    21661 agcaatttgt atctacacct gttacactgg atatttcgat ggatgggggc gtcccttatc
    21721 cagtagaagt agaagtaggc ggtgccacag tcttcgtctt acctgttgaa ggattccatc
    21781 aattttagga ggagctgagc tatgaatcaa gaaataaact tggctcaaat gcaaccgatc
    21841 gtgtatcagc agcttcaacg gagttttgag catgggcgta tcgctcatgc ttatcttttt
    21901 gaaggggaga aaggaacagg aaaacatgaa gtaggtatat ggcttgcaca gcacctgttc
    21961 tgtacccaaa tgaaagacaa tctgccttgt ggaaaatgca acaattgtca gcggatcgaa
    22021 aagaaagaac atccagatgt tttggttatt gaaccggaag gacaaaccat caaagtcgat
    22081 cagatcagac gtttgcagac ggaattcagt cgcagcggtt acgaatcaag aaaaaaagtt
    22141 tttttgatca aagaagctga aaaaatgaat agcagtgcgg caaacagttt gttgaaattc
    22201 ttggaagagc cccctggaga ttttttagcg gtgttagaaa cagatgcagt aggaagaatt
    22261 ctccctacta ttcaatctcg ttgtcaagtt ctgcattttc aagaactttc gaaagaagca
    22321 ctgatccatc gattgcaaga agagcagatt ccattggaaa aagcaaaact tttggctttc
    22381 ttaacaaaca gtttaggaaa agcagttgaa atctctcaag atgaatggtt taatgatgct
    22441 aaggacttga tttatcaatg gttcgtttac ttgcaaaagc aagatactca agcctttatc
    22501 tatgtccaaa aaaaattggt aaaatcattt aaagaaaaaa cacaacagtt tacaggatta
    22561 tctgtcttga tgttttatta tcaggaagca cagcgtcaag cagtagcaaa cggcgaatat
    22621 ggcaaagtga aacagataaa ccagataatt gaacggatat tgatcgcaga gcagaaacta
    22681 cgcagcaatg tcagttttca ggctgttgcg gaacagttcg ttttgcatac gattttttag
    22741 cttgccgcaa aggatgatga tcaaggagga tggaaatggt agaagtagta ggtgtccgat
    22801 atagagatgc aggtcatatc tattattttg caccaggaga ctctgaatat gtttatagtg
    22861 acaaggtatt agtcgaatct cagcaatcct tacatattgc tactgttgtc cttcctaaaa
    22921 aagagattga tcccaatgac cttcctgaag ctatccgtcc gatcttgcat aaagcgtcac
    22981 aagctgaatt agaaaaagac aaaaaaaaca aaaaagatgc ttatgatgca ttgaaaatcg
    23041 caaaagaaaa agtgcagttg cgtgagttgg aaatgaaaat ggtcaaagcc gaatacacat
    23101 ttgatcgaaa taaattgatt ttttctttca cagcggacgg gcgaattgac tttagagaat
    23161 tggtaaaaga tctggccgct gtatttcgta ctcgtattga gcttcgacaa attggtgtcc
    23221 gcgatgaagc aaagataata ggcggaattg gtccatgtgg gcgtccatta tgctgttctt
    23281 cttttttagg cgatttcgtg cctgtatcaa tcaaaatggc aaaagatcaa agtttatctt
    23341 tgaatccaac aaaaatttcc ggtttatgcg gacgcttgat gtgctgtctg caatatgaga
    23401 gcgaagaata cgagacagcg aaaaaagaac tccctgactt tggaaaagta attgctacac
    23461 ctgatggtaa agggaaagtc gtagggttga atctgcttag ccgtatcgtt agtattcggc
    23521 tgactggacg ggaaaatcct gtcgaatatg aatgggatga attgaaagaa tttatccaag
    23581 taggtgagat gaatggataa gagatcatta tacgacggat tgaatcaatt agaatcagag
    23641 ttgagaaata cattaacaga acttgttgaa atgaagcatg ctttgcatga gatcgtagaa
    23701 aaaaatacaa ctttggagat ggaaaatcaa cgtctgcggg aacacctgcg ggaattgaac
    23761 caagttgcac aagctccagc tgaaaatgtg aagcaggaac tttctaagtc tcgtatgaat
    23821 cttgaaaaaa tttatgaaga aggattccat gtctgctacg atttatatgg atcaagaagg
    23881 gacaatgacg agccttgtgc gttttgtcta gaagtgatct atcgcgaaag cggtcgatga
    23941 ccaataacaa gaggagactg gagcggagaa atactgctcc agtcttttta ttaaggagat
    24001 tgtgacagat gtataatcaa aaaagtttca agggaaatcc ggtatatggg gatttatatc
    24061 ttgtaccgac gccaattgga aatttagaag atatgagcta cagaagtgtt cgtatgctgc
    24121 aagaatccga tcttatcgca agtgaggata cgcgtaatac acaaaagtta ttgaatcatt
    24181 ttgaaatcca tacaccccaa aaaagtttac atgaacataa ttataaagaa cgtgtccctc
    24241 aattgattgc tttgttaaaa gaaggaaaaa cgattgctca agtcagtgac gcaggaatgc
    24301 cttccatcag cgatcctgga catgagctag ttttagcctg cataaaagaa gggatttctg
    24361 tgatctctat cccaggacct acagctggga tgacggcttt gattgcttca gggctagtgc
    24421 ctcaaccatt tttattttat ggcttccttc cacgtaagaa aaaagaacag aaagaaacat
    24481 tgaaaaagct aaaagaacag acagttacgc tgatttttta tgagtcacct tatcggatca
    24541 ctgctacatt gacgaatttc ttagaaattt ttggaaatcg ccaagtcgtt ttgtgtcggg
    24601 aattgacaaa ggtccatgaa gaatacctta gaggaagtac ggaagagcta ctcgcttata
    24661 tcgaagaaca tccagtaaag ggtgaatgtt gtctgcttgt agaagggaat ttgttcgaaa
    24721 tgccagtgac ggaagttgat gaagagttgg gaacgttaaa ggaacaagta gaagcaaaga
    24781 tcgcttcagg cgaaaaacca aatgctgcta tcaaagcagt agcgctgaaa aatgggctga
    24841 aaaaacaaga agtctaccgt cagtaccatg aattagactg agatagtata agttgtctat
    24901 aatgaaagac taatgaggaa atcagctact aacttttcaa atctcttgaa cgcgaacgaa
    24961 tgttcttgta taatgaataa gaggtgagga tgatggagct gcaatttcct ttacataatg
    25021 atacatcaag aaaaatcatt catattgata tggatgcttt tttcgcttcg gttgaagaac
    25081 gagatcatcc tgagtttcgc ggacatccat tagtaattgc cagacacccc agtgacactg
    25141 gtgggcgtgg agtagtgact acagcaaatt atgaagcaag aaaattcgga attcattcag
    25201 caatgagtgc acaaaaagcg tatgagcttt gtccgcaagc catctttaag cctggcgacc
    25261 atcataaata cagcgatatt tccaaacaag tacgggaagt attctatcgt tatacagata
    25321 ttgtggaacc agttagtatc gatgaagcgt atcttgatgt gaccgaaaat aaaatcaatt
    25381 gcaagtcagc catcaaaatc gcccgaatga tccaagcaga tatttgggag agtgtccagc
    25441 taacatgttc tgcgggagta agttacaata agtttttggc caaattagca tcagactatc
    25501 aaaaacctag aggaatcacg gtagttgccc ctcaagacgc tgtgccattt ttaaaagctt
    25561 tgccaattga aaaatttcat ggggtaggga agaaaactgt acctagaatg catgagctag
    25621 ggatttatac aggaaaagac ctatatgaat gcacagaaat gatgttgatt cgaaattttg
    25681 gaaaaatggg ctattctctt tatcgtaaag tcagaggaat ccatgattct ccagtaaacg
    25741 ttacaagaga gcgaaaatct gtgggaaaag agcatactta tgggcagccg ttgcaaacgg
    25801 aagaagcagt gctgactcag ttgcgccaac tagctgaaaa agtagaagaa gcactgagaa
    25861 gagtccaaaa acatgggaaa acagtagttt tgaaggtcag gtacacggac tattctactg
    25921 tgaccaaaag agtgacattg ccagaatata tctataaaaa agaagcactc ttttatcaag
    25981 caagccttat atgggaggaa atcttgggag tagaaaaagg aatccgattg ttagggatta
    26041 ctttgacgaa tctggacccg atgacttatg aaaacatcgt ccttccatta tgggaaaatc
    26101 aagaaatata aaaacagctt tgagagttga aaccggaaat ccgttttctc tcccaaagct
    26161 gtttttatat caagtcttgc tttttgattt gttcataggt ttctgttgca tccgtacatt
    26221 tttcccaaat cacaacttga ttggattcta aagatttttg gacgtcaatg atccgctggt
    26281 tgctacttcc gcgaaattgt agttttaggt cacgtttgct taattcaaag cgtccgtcaa
    26341 caaggatatc gatttgcgaa aggagttcta atttatcttc tgaatcttgt agcaattcat
    26401 caaaggtata gcctgtccat gaccagatat cttttgtcgt accgaactct tttcggatac
    26461 gtcgtacgac ttgcagacaa acatctgtat tcaaaaaagg ttcgccacca agtaaagtta
    26521 atccttgcac gtaatcgttg cgcaagtcag cgatgatttg ttcttctaat tcaggtgtga
    26581 atggtttgcc atatcggaaa ttttgtgcag ctttgttgaa gcagccttcg caggcaaata
    26641 agcagccact aacgtaaaga ctgttgcgga caccttcacc atcgacaaaa ttaaacggtt
    26701 tgtagtcagc aatatagttt tggctgtagt cttttgctaa ccattctttt ggttttggat
    26761 ttctcataaa aaaacttctt tcatttaagg ttcaaagata tagctattga agataaaatc
    26821 tactcgacca cataaataag aacaaactga cctaaatcca ttgttcaaga aagcttgttc
    26881 ttattttgat agagggcgtt caaagataca aagccatcga aagagggtgg tgaaaaaaga
    26941 cttgtcatag cctcttattc tgaataaacg gtgttcaaaa gcttgcatct gcagtaataa
    27001 gcccaactaa acgaaaaata tgaggaacta tttttgttta gctattctta ttacttgatg
    27061 caggacagct ttttcacaac ctcatttcat gtgtttcaca cgtgaggaaa tttctttatg
    27121 gcggccatgt accatcggac gagcttgcgg atttcctaaa tagccacaag tacgtttgac
    27181 tacatcacat gtttttggat catgatttcc acattctgga cattcgaacc ctcgttctgt
    27241 cggtttgaaa tcaccttcga aaccacattg atagcaatga tcgattggcg tattcgtacc
    27301 aagatacccg attttgtcat atgcaaaatc ccatacagct tctaaagctt tcggattttg
    27361 ttggagcata gggtattcac agtagtggat gaatccacct gaacaatatt ttggataatc
    27421 cttctcaaat tccaattttt caaatggcgt tgggttttta cgcacatcat agtggaaact
    27481 gtttgtataa tattctttat ctgtgatatt ttcgatgatg ccgaattttt ctgtatccaa
    27541 acggcagaaa cggtcagtta gactttcact tggagttgaa tagacactga aatgataacc
    27601 atattcatcc ccccatgcat ctgcattagc ttttaattct ttgacgatat caagtgtgaa
    27661 agcttttgct tctgggtttg tttcccattc gccaccgaag aaagctgaag caacttcata
    27721 caatccaatg tagcctaacg atacagtagc tcgacggttt ttgaataatt cattgacaga
    27781 atcggtacgt gaaagacgtt tcccgaaagc accatacata tataaaattg gcgcatttgc
    27841 tggaatggct tctttacaac gttctacacg atagagaagc gcatctttca tgatttctaa
    27901 gcggtcagac agtaactgcc agaatttatt ctgatctcct tttgattcga tcgcgattct
    27961 tggcaaattc aatgtaacaa cacctaaatt cattcgtccg acatttactt cttgaccatt
    28021 ttcgtctttc cacccttgca agaatgaacg acagcccata ggtactttga aacttccagt
    28081 taattcaacg attttatcgt aattcagaat atcaggatac attcttttcg ttgcacattc
    28141 caatgctagt tgtttgatgt cgtaattagg atcttcaggg tttaagttca cacctttttt
    28201 caaagtgaag atcagtttag ggaagatagc tgtccgcttt tcactcccta atccattgat
    28261 tcgaatctgt aaaattgctt tttgaatttc tctttcaaac cagttttctc ctagtccaaa
    28321 tcctaatgaa gtgaaaggag tttgtccatt tgaagtaaat aaggtattga tttcatattc
    28381 caaactttgc atagcatcga agatatcttt tttggttttt gcttttgcat attctttttg
    28441 gcgttctggc gaatcgatcc attcttccgc gtcttttaga tgttttttat agttcaattc
    28501 tgcaaaagga gcaagcaatt catctgttcg gtcggcagaa cagccaccat attgacttga
    28561 cgctacattg gcaataatct gtgagatttg tgctgtggcg gtctggatag attttggcga
    28621 ttcgacttcg gcattgccga ttttgaaccc attgttcagc atgcctttga aatcaatcaa
    28681 gcagcaattc gtcatcggtg tataaggatg ataatccaaa tcatggtaat ggatatcgcc
    28741 tttttgatgc gcatttgcta tatgtggtgg caacattttc agtccgattg attttccaac
    28801 gatcccggct gtcagatctc gctgtgtatt gaaaacatta ctgtctttat ttgcgttttc
    28861 gttcacgaca gtttggtcct tgttgattag cttttcaatc gaaaagttga tatctgttgc
    28921 tttactgcga gcaaaatctc gtttggtacg ataattgatg tactcttctg ctaattcata
    28981 ttcgtttttt tctagtaata tatgttccac gatgttttga atctcataga ttttgacatt
    29041 ttcagcaaac cggcttttga tttcttggac aaccaagtct gcaatcattt gaattttttc
    29101 ataagtaagt ggtgataatg agccgtgaat tttcttttct gctttgatca gtgcttcatt
    29161 gatcttttgt tcatcgaaat caacatggcg gccatcgcgt ttgatgactt tcatctggga
    29221 tgtctctggc tgctgagtaa cgacagtttt ttcttcataa tttactttca ttatcttcaa
    29281 ctcctgctaa attgttcttg tctatcataa aacaaaatcg gggaaggagc aactaaatat
    29341 cggctatata tagtattcaa tgtgctgtat aattggattt ctatactata tgtagtgtat
    29401 attttattgt gaaaaaaggt gaaaacttgt agctggtgga tttgtttttt gcatatttaa
    29461 ttttgctgaa taaaataatt gaataaaatt ttttattcag tccaagtaaa agtgacaaaa
    29521 acataagagc aaattattcg tagatatgct actatgcaaa taaatagaaa tgaaaaatga
    29581 aagaggagga gaagaattgg aaaagatgat cgaagtgaaa catctaagta aaagctacgg
    29641 cagcaaactt tctcccgtca aagccttaga taatatctca ttcacagtag aagaaggcga
    29701 gtttgttggg attatgggcc caagtggtgc aggaaagaca acgctgatga atatcttgtc
    29761 cacgatcaat ttgcctacga tgggcagtgt ctcgatccaa ggaaaagaaa tcacaaaaat
    29821 gaaaaacagt gagctcagtg actttcgtcg aaaaaaacta ggattcattt ttcaagagtt
    29881 caatttgatc gataccctaa atgcaaaaga caatatccta ttgcccctag cagttgaacg
    29941 gatgactaaa gaggaaatgg aaaagagagt aagacatgta gctcaattgt tgaatataga
    30001 agaactgctc aaacggtatc ccgatgaact ttctgtcgga cagagacaac gaattgcagc
    30061 tgctagagca cttgttgttc agccacaagt gatttttgca gatgaaccaa ctggctcttt
    30121 ggactcgaag tcggctacag aattgttgaa ctacttgaag accatgaatc aaaaagaaaa
    30181 agcaacgata ctgttagtca cccatgatcc atatacagca agctactgcg atagaatttt
    30241 atttattaaa gatggtgtga ttttttctga agttgtcaga agagggacaa gaagagagtt
    30301 ctttgagaaa gtaatagaca tgcaggcgac gattggcgga ggaggaaaaa tgaatgctgt
    30361 ataaactagc cgttaaaagt ttttttcggc agtcacgagg ctatcttgtt tattttttca
    30421 gtttgacttt atccgtaatg atctattatt ccttcagcgc aatgacctac gatcaatctt
    30481 tagtcagaag agcaagtcaa gatacttcga tagatggtgg attgagtttt ggtggattta
    30541 tcattactgt tgttttgctt tttttcgtat tcagtgccaa ccgatttttc ttgaatagaa
    30601 ggcaaaagga aataggcatc tatcagcttt ttggtatgaa taaactgcag atatcaggta
    30661 tatatgtgtt agaaatcatg ataattggat tgttcgcctg tatttctggg atattactcg
    30721 gtattatttt ttccaaactc ttttcaatga ttttggttcg aatgatggat atggatctta
    30781 ctagtccatt ttttatctcc attccatcag tagtagatac tttatttgct tttttcatca
    30841 tccttttagc agtatctgta tattcaatat ggaaaatatg gcgctatcca atgattcgaa
    30901 gttttggaga aaaagaacaa gcagatagtt caacaatgcg cttcaggaca aggcaccgat
    30961 tactcgcagt catcgggtta ctacttttaa cgagcggata ttttggagct agtcatttta
    31021 gggaaatcac ttcatggatg atcagttatc attatcttga tacattagtt ttcttttttc
    31081 cttttttaat ttttttcgtt tgtgtggtcg gtacttattt gtttttctgt tatacatttc
    31141 ggttattttt gatcatattg agtaaatgga agatttatta ctatggtatc aattttttga
    31201 taatgggtaa cacgcaagtt catttgctca aaagttggcg tacaaattct ctgattacag
    31261 tcattctcgg aatttcttta atgacaattg gcggcacggc tagtatctct actattttat
    31321 cctaccagga gaaagtttcc tccccgatgg actatcagtt tgatgttgag acagagaaaa
    31381 aaatcaagcc gattctagca gaggaagggc agaagataac taaagaaatc accttgcatt
    31441 ataaagtcat tgggagtatc tatagtcaaa atctcgaaga acctatgttt caaactgcta
    31501 atctgatcac ggaaacacaa tatcaagcct tcagaaaagt taatccagat ttgccgaaca
    31561 tttctttgaa atcacctggt catactgttt tgcttgatca aatgcaaact ctttttcgga
    31621 atatttcatt atatggatct agtattggac tgccagataa tcaagaattg aagattcaac
    31681 aaatgcttcc aagtgtatta ggtgacgaaa caatggtata tctaagtcct gtactgattg
    31741 tagatgatac cgtttttgaa aaagcaaaag gtgtggatta tcaagtagtc aatgtagatg
    31801 tatcaggtgg aaataatgaa aaaatagatg aacgactgag caatgacctg tcgataaagt
    31861 ggctcaatgc tgtttattat tcttatgata tcgtaaatgg aagtctacaa ggagaaatta
    31921 gcacaaagaa acttccggat gaccaaatga aggaagcaca atttagccaa gaaagctctc
    31981 gtttgaattt ctcttccagc tattcgaaat tgcgtgcagt taatcggcga atcggtatca
    32041 atatttttgt cacactattt gtcgggatga tcgtgattat tgctactggt agtattttga
    32101 cagtccggca attagcagaa gcagaggaag aaaaagaaaa ttatcaccta ttgactaagc
    32161 tggggattcc acaaagaagg atcagacgga tgatcttcga gcaaaacgca cttaccttct
    32221 ttccacccat gatattaggg atcactcacg cagtatttgc aatcaatgtc ttcactcagt
    32281 atataaaaac tgccgattac tggttggctt atcttgtgag tggcttattg atccttatct
    32341 atcttttgct ttatgtcatt acgagtcagt tatattgtcg gattatcgag gaatgatata
    32401 cggaataaga caagataata aatagaacta aattttgaat accatttatt tggaataaga
    32461 ggttgtgaca cgatttttgt cacaacctct ttatttttta tattttttaa aagtacgaga
    32521 aattataaag aaaaaagcag tgatttgatg tgctattttt ataaaagcta tgtgacaaaa
    32581 ctgttagtaa aaaaacggtt tctgtaagct agacacatgt ttctttttgt caaaattagt
    32641 tacactttgt gtgaggttat atgaggaggg aatccaatga acaaatggcg ttctaatcca
    32701 aaattgaatt attggggaaa attcatcatt cgaacaattt tttatagtgc agtactgctg
    32761 ttcctgattt atctgtatca ctataaaaat attcaaggag gaacttttat ctataacgaa
    32821 ttttaggagg aagagtatgg aaatcaaaac gattattgaa gcaatcgatg attggggaat
    32881 aaatgaacct gatcgagttg cttatcaggc ggatgagacg catacttttg gtgaattgaa
    32941 acaagcatca gacgcacttg cttattattt agaaaaaaag gtagaaggcg atggaccaat
    33001 tgtcgttttt ggcaatttgg agtttgaaat gattgtctca tttctagggg ttgtcaaatc
    33061 tggccatgcc tatttgccga ttgaagagca tacacccaag gagcgtatcc tatcgatctt
    33121 tcgggtggct aaaccatcta tggtcatcag tatcggggac tggatagaag atattccttc
    33181 ggttcctgtc ataacaaaaa aagaatttca agaaatcaag aagataccag taggatttta
    33241 tcccagaaat agcgtaaaag gtacagaaaa tttttatatt atttttactt ctggtactac
    33301 tggagaacca aaaggcgtac aaatcagtca tgataatcta gtcagctttg ttcaatggac
    33361 gatggaagac tttgggatca agcctggtat gcatttctta gcccaagcgc cattttcttt
    33421 tgatttatcg gtattttcta tttatcctgc attagtttct ggcggaatgc tgaagccgtt
    33481 aaataaagca gttgttcaag actttcgtca attattcgcg acattgccag ccttgaagtt
    33541 gaatgtgtgg gtttctactc catcatttat ggatatttgc ttgatggaac ctacattcaa
    33601 tggagaaaat gtgccggaat tggacatgtt tttattttgc ggagaagaat tgacgaaaaa
    33661 aactgcagaa agtctacttg atcgattccc aaatgcacgg atatataata cttacggtcc
    33721 aacagaagca actgtagcta tttcaagtat tcagattgac cagcatgtac tagatagtta
    33781 tgaccgacta ccgattggct acgtaaaaaa agacacgaaa gtttctattg tccaagaagg
    33841 taaacctgtt tcaaaaggag aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa
    33901 aggttacctg aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc
    33961 aagttaccat acaggggatg caggtagact agatgaatat gggcttttat tttatgaagg
    34021 acgtatggat cagcaggtca aattacatgg ataccggatt gaactcgggg atatcgaaca
    34081 ttatctgctc caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac
    34141 aaaagtacag cagctggttg cttttgtcgt attagaaaac aaaacgaaac agccggattt
    34201 tcagttgact aaatctatca aagaacaatt actagaagtt gtaatggatt atatgattcc
    34261 tcagaagttc cattatgtcg atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa
    34321 aaaattaaca gctgaggtga atcctgaatg atgttacctc atatgatccc ttatgcagag
    34381 ccgttttatt tttttctttt agccattgcg cttgtaccga tcgttctatc tttattgata
    34441 tgggaaaaaa gattaccagt ttatcaatca gcagtgacac ttttcttttt atttgtcagc
    34501 tttggaggta attattggca acagggagcg gcgctgattg ggtatgtttt gtggcagacg
    34561 atacttgtat ggggctattt tgtttatcgg caaaggaaaa atcagacagg atggtttttt
    34621 gccgccgtta ttttagcgat aattccactg tttttaacga aagtgactcc tttttttgaa
    34681 catggaaaag catcgttact tggtttttta ggaatctctt atttaacctt taaatctgta
    34741 caaatgatca tggaaataag agatggcatg atcaaaacat ataagccatt tcgttatatc
    34801 cagtttctgc tgttctttcc aacaatctct tctggaccaa tcgatcgcta taggagattt
    34861 gaaaaagatc agctccatcc gcctgttcgt gataaatatg ttgatctttt gggaaaaggc
    34921 gtccaaaata tttttgtcgg ttttttatat aaattcatta tcggttatta ttttggtcaa
    34981 gtactgcttc ctgtagtggg acgcattgcc ttacagcatg gtggtatttc atgggcatta
    35041 gtaggttata tgtacgtgta cagtatgtat ttgttttttg attttgctgg ctatagctta
    35101 tttgcagtcg gtacaagcta catgatggga tatcagacac caatcaattt caataaacct
    35161 tttttatcat ggaatatcaa agaattctgg aatcgttggc atatgacgct gtccttttgg
    35221 ttccgtgatt atgtatatat gcgattgatg ttcttcatga tgaagaaaaa attttttaaa
    35281 agcaagatcg taatgtcaaa tatcggttat tttgctttgt ttcttctaat gggtatatgg
    35341 catggattga cgtggtacta catcgtatat ggactgtatc atgcttgtct gatctgtctg
    35401 acagatgcat ggatccgatt taagaagaag cataaagagc agttgccttc taacaaattt
    35461 acgcatggct tagccgtatt tttgacattt caagcagtat gttttagctt cctgattttt
    35521 tcaggatttt tagataaact tttttttagc taactaatag tgacatatta atttatataa
    35581 caaaggagaa aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa ataacaggaa
    35641 cagatgaagt aagaaaaaat cgtgatattg atctatttga tgaagggttg cttgattctt
    35701 tagcatctgt tcaattatta gtcgagctag acggagagct ggatatccaa gtccctgtat
    35761 ctgaattcga gcgagaggat tggagcacac cgaataaaat cattcaacaa gcaactgcat
    35821 tgaagggttg agtatgaaaa agaaactttt tgctattttc ggcccggttg ttttagctgt
    35881 tggcttactg tttgtgtttt ttacctcctc ctatcaagta gatatctcta atccagaaac
    35941 gattagacgt gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa
    36001 ttatgcatta tctgaaaaac aatatattcc tttttttggc tcaagtgaat taagcagaat
    36061 cagtccattt catccgtctg ttctggcaga aaaataccag cgaaactatc gtccattttt
    36121 gctgggagca cccgggacac aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga
    36181 tgctatgaaa aacaagaaag tcgtctttat tatttctcct cagtggttcg ttaaaaatgg
    36241 tgtaaaaact gattacttta atacgtatta ttctgaactt caaacatacg actggttgtt
    36301 ttcaatgaag aaagtaacgc ctgctgatcg ctatttagct agaagattat taactttctc
    36361 aaaagtaaaa gagaatgata cgttgacggc gattctgcaa acgatcaaaa aagggaagct
    36421 acctttacct gaatctttga atcagctgcg cagccaatgg aacatgctga agagagagga
    36481 tgaagtattc agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa
    36541 acgattacca aaacaatacc aagaaacaga attatccatt ttagcaaatc aaattggaga
    36601 acgagaaacg acgaataatc cattcgggtt gaaaaacgat ttttatacgc atcgtatccg
    36661 agcccacgag ccagagctca aacagtcaca aaaaaattgg gattatcgtt tttcgccaga
    36721 attttctgat tttcagctgg tcttggatca attagcaaaa aaccataacg aagtattgtt
    36781 catcatccca ccggttaacg agaaatggag cgattatacc ggactatctc aagaaatgtt
    36841 acaaggattt gccaaaaaga tcaaatttca attaaatagc caaggattca atcgaatagc
    36901 tgattttgtc aatcaagcgg gtaccaacta ttttatggaa gacacgatcc atctaggctg
    36961 gaaaggctgg ttggctgcag atcaacagat cagaccgttt ttagaagaaa atcatattac
    37021 tgctagtaag tatcatcttg acgatgcttt ttttagcaaa tcttggcaac atcaaatacc
    37081 agataaacta caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt
    37141 tttgaatatt ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa atcataagca
    37201 atggtataat cacaatatga atatagaaat gggggaaaac atttggatac aaaagaagaa
    37261 cagctactta ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga
    37321 gaagtattca gagaatatgc caaaccattc gctgatgata ttttttatga cggtttaggc
    37381 agtgtgattg ccaaacatgg tgctggcgga ggccccaagg tctttatttc tggacatatg
    37441 gacgaagttg gttttatggt aacaaaaata accgaaaaag gattcttgga attccaaact
    37501 cttggaggtt ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa
    37561 gggaaaatcg tccacggtgt gattggttcc aaaccaccac atgttttaac acctcaagta
    37621 agaaataaac cttatgaaat aaaggatatg ttcattgata ttggcgcatc aagccaagaa
    37681 gaagccaaag agtggggcat acgtcctgga gatatggtca ctccatatat tgaatacaaa
    37741 cgaatgaatg gcagtaaata tttactggca aaagcatggg ataatcgtat cgggacagct
    37801 gtttctctgc gagtattaga gaatctatct aaagaagcac acccaaatgt tttattcgca
    37861 ggaagcgatg tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac acaccttgta
    37921 aatcccgata ttgcttttgc tttagatact ggtacagcag gagacactcc aggaatgaca
    37981 ccaaaagaag cagattcgat tttaggaaaa ggcccacaga ttttgatttt tgatgcatct
    38041 atgatcccgc ataaaaaatt attgaatttt gttattgatg tagcagagga aatggatatt
    38101 ccattccaat atacagtgat ttctggcgga ggaactgatg caggacaaat gcatttgaca
    38161 agagatggtg ttccgtctgt agccattacc gttcctgttc gttatctgca ttcccataca
    38221 tcagtgattc atgaagatga ttatttgaat acagtgaaac tagtcacaga attagtgaaa
    38281 cggcttgata gtgaaacagt tgaaaaaatt cggagttatg aataatgaat aatagaatac
    38341 ctatttctga atatcggaaa aaagcacgag tttctttgga aaatcaatgg ggaatcaata
    38401 cttggcttgt tttcttatct ataatattta cgagcgtcat ccagaacttt tttgacggtt
    38461 tcagtccttt tccggaagaa agcatgcagc agcacttatt gagttttcta gtcgataatt
    38521 tacttttatt cgtattcaac tatgcattgt tttacattgc gctagtagtt gtacggggtg
    38581 gcagaccaaa aagtaacctt ttattttcag ttttccaaag aaagtatttt ggttcgatac
    38641 ttggcatcaa tttgatcaat tcagtcatca actggctact caacagttta atctttctgc
    38701 ccatgttttt aatcagcgga gtgagtactt atatgcagct gcttttcagt tcaaatgcgg
    38761 tacctacggg tgtcatgcca gaagattttc ttgatatcag ctttgtttta tcgatgatag
    38821 ggatgatttt tgtttcagca ctcatcatga gtattgttgg cggtttattc caatttgctg
    38881 catggacaaa gttcgactat cctgatttgt cggttattca atgtttgaaa tatggctggt
    38941 ttttattgaa agatagaatt ggtacgtata ttttgttgca gctgtctttt atcggctggt
    39001 atttgctagg agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg
    39061 tgtcagttgc agaattttat gaacaagcac ggattgaaaa aggaggtcca gacgcctatt
    39121 ttacttagca tgtaagcagt ttattcatca aaaaaataac ttttgtggta ggataaatct
    39181 ttgaggggga atgtacgatg aaattaactg ttttaggatg cttaggagcg tatccttata
    39241 aaaatcaagg gacaacaggt tatttgcttc aatctgacgg gttcaatttg ttgatcgatg
    39301 caggtagtgc tacgctgatc aaattacaag aacaccttga tccacttgat ctagatgctg
    39361 tgatccttag ccattatcat catgatcata ttgctgacct tggtgtatta cagtattact
    39421 ggcaacttca tcctaagaga aatccggaga cattgctttc aatttatgga cacacaaaag
    39481 attccattca ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat ccttattttg
    39541 aagctgaaga attaaaaatt ggtccttttc tgattacttt tatggaaaca atccatccag
    39601 tagtatgtta cgccatgcgt atcgtagaag aaacaacggg gaaagtattc gtatttacag
    39661 gtgattcagg ttatttggaa agtttcgctg attttgcgaa agatgctgat ttgtttctag
    39721 cagataccta tctttttgaa ggaaatgaac atcatcatgc ccatttcact tcaaaagaag
    39781 ctggagaact tgcgaaatca gctaacgtaa agaaacttgt acttacacac ctacctcagt
    39841 ttggtgattt agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata
    39901 tagaacttgc tgcagttgac aaagtgttta acatttagga ggaagaacat gatttttgta
    39961 ccaaatgaaa accacgaccc aagaatcaat ttagcaattg aacaatattt gctgcaagaa
    40021 atgcctttag atgaacctat tttgctcttt tatatcaatg aaccttcaat catcatcggc
    40081 agaaaccaaa atacgcttga agaaatcaat cgagattatg tggaagaaaa tggcattcat
    40141 gttgtccgtc gcttaagtgg aggcggagct gtctaccatg attttggaaa cctgaacttt
    40201 agttttatca tgcctgatga tggggattca ttccgtgact ttgcgaaagt aacacagcca
    40261 atcatccaag cacttcatga attgggtgtt gctggggcgg aattgaaagg ccgaaatgat
    40321 ctagtcattg atggcatgaa attttctggc aacgcgatgt atgctaccaa cggtcggatg
    40381 tttgctcatg gaacaattat gttcgactct gatatcaatg aagtagtcaa tgcattaaaa
    40441 gtaaaaaaag acaaaatcga atcaaaagga atcaaatcga tccgttctcg tgtaacgaat
    40501 atcaaaccat ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa
    40561 atcttgttga aaattttcgg cgtagagtca gtagaagaag taaaaacgta cgaattgact
    40621 gatgaagatt ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa ttgggattgg
    40681 aattacggga agtctccaga tttcaatttc tctcgccaaa aacgtttttc tatcggttcg
    40741 attgaagtcc atttgaatgt ctcagaagga atgatcaaag aagcaaaaat ctttggcgat
    40801 ttcttcggac taggagatat ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat
    40861 aaagcatctc tatcaaaaat agtcagccaa attgatgtga agaaatattt tggtgcgata
    40921 gaaccagaag aattgcttac cttactttat tgaaaataaa aaatagcgga atccacatat
    40981 atggattccg ctatttttta tacagtattc gggtaatttc gagagagttg aacagtgatc
    41041 cgatcaatga aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct
    41101 ttcatcatca ttaagtcaaa acgttcctga tcatctaatt cttctgtgat gcctcgacta
    41161 aataggtttg tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt
    41221 tcgtgtatcg acaaataaat ttttcgatac agtggatttt cgatggcatg agtttctcta
    41281 aaggaatgct ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt gataggaagt
    41341 tcttcgggaa gatctgcttg gaaggttatc tgaatattcc gtttggcagc taacttctgg
    41401 taacgcgcta agataagatt tgtcacctgg tggttgcaaa aagaacacag catttcgaaa
    41461 ttttttgctt cttctaatat cttttccaca tagtttttag cttttgtttg gtccgtatgc
    41521 attaaatctt gcagattctc catctcttgg acaaattcca ggcgaagtgt atttacttct
    41581 tggatatgct gtcgaagctg ctcataatgt tgctccaaca aatcattttt gatttttaag
    41641 aaatctactt gtctccgatc tttgtcggta tttaagatgt gatcgattag attgtagctg
    41701 ataataacga ttagaatgat aatatataaa atcgtagacc aattgatcgt tgtttgatag
    41761 atacctaatg ccgtttggat gtaaatcaag caatggataa tggctaccaa gacgatccaa
    41821 ggtgtacaag ctacataaaa acggttattc ttatatgctt cgccaatgca tgcaagtgaa
    41881 gtgactaaag tgcttataac actagcaata ttagcaatcg gcattaattc aggaagttct
    41941 gcaggagtaa aagttgcata aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa
    42001 aaaattccgt accatttttt atagtaaaac attcggctcc aatagtagca gatgatgaaa
    42061 gctgacgtta agataataaa taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga
    42121 ataccactta aaatatcttc tgatacagct tcaaatccta atgccaaagc aaagcaactt
    42181 aagagaataa gtgagtattc aagacgtttt tgtttataca tcaagtagat cgtatagaaa
    42241 aaggtcccta tagcgataaa aacagcgatt atcatcgtca atacttgcgg cactgattgt
    42301 gaataaataa aactaatcaa tggtccagtt gttcctagat aaactttcgg tggcagtcca
    42361 gcatagttct gataaggaga tgagactgta agggtcaatt tttttccgat ataatttgct
    42421 ggtaaatcta tcacagccaa tgagagtccg ggattgcttt cttcctgata atctttccgt
    42481 tggtataaaa tcttttcgcc tagatataca gtaatccatt gatggtttcc tttgattagt
    42541 aaatttgctt cgtccaacgg tttttcaagt gtcgtttcca taatgaatg
//
LOCUS       DACEZN010000007        40171 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEZN010000007 DACEZN010000000
VERSION     DACEZN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.007"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IXH60_000264"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IXH60_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1989235.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IXH60_000265"
     CDS             complement(322..891)
                     /locus_tag="IXH60_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1989236.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IXH60_000266"
     CDS             1077..1979
                     /locus_tag="IXH60_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1989237.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IXH60_000267"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IXH60_000267"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1989238.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IXH60_000268"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IXH60_000268"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1989239.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IXH60_000269"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IXH60_000269"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1989240.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IXH60_000270"
     CDS             8547..8732
                     /locus_tag="IXH60_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1989241.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IXH60_000271"
     CDS             complement(8794..9990)
                     /locus_tag="IXH60_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1989242.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IXH60_000272"
     CDS             complement(10160..11596)
                     /locus_tag="IXH60_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1989243.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IXH60_000273"
     CDS             11889..12698
                     /locus_tag="IXH60_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1989244.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="IXH60_000274"
     CDS             complement(12884..15037)
                     /locus_tag="IXH60_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1989245.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15331..16179)
                     /locus_tag="IXH60_000275"
     CDS             complement(15331..16179)
                     /locus_tag="IXH60_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1989246.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16403..18343)
                     /gene="thrS"
                     /locus_tag="IXH60_000276"
     CDS             complement(16403..18343)
                     /gene="thrS"
                     /locus_tag="IXH60_000276"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1989247.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18680..18859)
                     /locus_tag="IXH60_000277"
     CDS             complement(18680..18859)
                     /locus_tag="IXH60_000277"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1989248.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(18906..20729)
                     /gene="cadA"
                     /locus_tag="IXH60_000278"
     CDS             complement(18906..20729)
                     /gene="cadA"
                     /locus_tag="IXH60_000278"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1989249.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21007..23286
                     /locus_tag="IXH60_000279"
     CDS             21007..23286
                     /locus_tag="IXH60_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1989250.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23379..24026)
                     /locus_tag="IXH60_000280"
     CDS             complement(23379..24026)
                     /locus_tag="IXH60_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989251.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24205..24924)
                     /locus_tag="IXH60_000281"
     CDS             complement(24205..24924)
                     /locus_tag="IXH60_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1989252.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25055..26206)
                     /locus_tag="IXH60_000282"
     CDS             complement(25055..26206)
                     /locus_tag="IXH60_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1989253.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26423..27163)
                     /locus_tag="IXH60_000283"
     CDS             complement(26423..27163)
                     /locus_tag="IXH60_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1989254.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27167..27490)
                     /gene="rsfS"
                     /locus_tag="IXH60_000284"
     CDS             complement(27167..27490)
                     /gene="rsfS"
                     /locus_tag="IXH60_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1989255.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27547..28140)
                     /locus_tag="IXH60_000285"
     CDS             complement(27547..28140)
                     /locus_tag="IXH60_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1989256.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28124..28771)
                     /locus_tag="IXH60_000286"
     CDS             complement(28124..28771)
                     /locus_tag="IXH60_000286"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1989257.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28784..29095)
                     /gene="yhbY"
                     /locus_tag="IXH60_000287"
     CDS             complement(28784..29095)
                     /gene="yhbY"
                     /locus_tag="IXH60_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1989258.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29111..30220)
                     /gene="yqeH"
                     /locus_tag="IXH60_000288"
     CDS             complement(29111..30220)
                     /gene="yqeH"
                     /locus_tag="IXH60_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1989259.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30217..30744)
                     /locus_tag="IXH60_000289"
     CDS             complement(30217..30744)
                     /locus_tag="IXH60_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1989260.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(30924..31709)
                     /locus_tag="IXH60_000290"
     CDS             complement(30924..31709)
                     /locus_tag="IXH60_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1989261.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31702..32571)
                     /locus_tag="IXH60_000291"
     CDS             complement(31702..32571)
                     /locus_tag="IXH60_000291"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1989262.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32572..33951)
                     /locus_tag="IXH60_000292"
     CDS             complement(32572..33951)
                     /locus_tag="IXH60_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1989263.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(33959..34378)
                     /gene="fabZ"
                     /locus_tag="IXH60_000293"
     CDS             complement(33959..34378)
                     /gene="fabZ"
                     /locus_tag="IXH60_000293"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1989264.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKTSAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34403..34879)
                     /gene="accB"
                     /locus_tag="IXH60_000294"
     CDS             complement(34403..34879)
                     /gene="accB"
                     /locus_tag="IXH60_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1989265.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34882..36117)
                     /gene="fabF"
                     /locus_tag="IXH60_000295"
     CDS             complement(34882..36117)
                     /gene="fabF"
                     /locus_tag="IXH60_000295"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1989266.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36136..36873)
                     /gene="fabG"
                     /locus_tag="IXH60_000296"
     CDS             complement(36136..36873)
                     /gene="fabG"
                     /locus_tag="IXH60_000296"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1989267.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36885..37802)
                     /gene="fabD"
                     /locus_tag="IXH60_000297"
     CDS             complement(36885..37802)
                     /gene="fabD"
                     /locus_tag="IXH60_000297"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1989268.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(37909..38133)
                     /locus_tag="IXH60_000298"
     CDS             complement(37909..38133)
                     /locus_tag="IXH60_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1989269.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38186..39148)
                     /locus_tag="IXH60_000299"
     CDS             complement(38186..39148)
                     /locus_tag="IXH60_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1989270.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39158..39598)
                     /locus_tag="IXH60_000300"
     CDS             complement(39158..39598)
                     /locus_tag="IXH60_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1989271.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            39904..>40171
                     /locus_tag="IXH60_000301"
     CDS             39904..>40171
                     /locus_tag="IXH60_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1989272.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYN"
BASE COUNT        11327 a         8735 c         7006 g        13103 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag
    15121 aaattcttta cctaatttca agataaaatc aactttttgt ttcacagcaa tcacttaaag
    15181 ttacatatcc aactattttt ttcattaaaa aataaactta tttttgaaaa ttatgttttc
    15241 ttaagtcgtg aaacactttg atggatcatt tatttgtgga aaactacgtt ttgttattct
    15301 aaaaaactta tataaaaaat agtaaggttt ttatttcgaa tccataaaat attcttggat
    15361 tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca tatagaaata
    15421 aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg cacttcgatt
    15481 cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc taagcgggtt
    15541 atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca gctcggctaa
    15601 tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt tctttttctc
    15661 attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga ttggcttacc
    15721 gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc caaccccata
    15781 taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg tttcttcttc
    15841 tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat
    15901 agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc cagtctgtag
    15961 ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt ttttggaaaa
    16021 acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag ctgtcattgt
    16081 agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg ctgaagaata
    16141 atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca cccttttaat
    16201 gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac tggataaaaa
    16261 acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag attctattga
    16321 gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc tatctactac
    16381 ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact tctgcttcca
    16441 tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca taacgacgta
    16501 cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa gggatctttt
    16561 gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca acttctacac
    16621 gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca tgtgcatcaa
    16681 cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt
    16741 atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg cggtggataa
    16801 ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca aagcgctctg
    16861 gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga gctgttttga
    16921 cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta
    16981 gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac attgcgtcat
    17041 catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa cgataatctg
    17101 taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta aattcatctt
    17161 tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct cgtacacgtt
    17221 gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat tcagcgatac
    17281 gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg tgatgagggc
    17341 agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc gggaacatgt
    17401 cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct cccattattg
    17461 gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa cgttcgatcg
    17521 tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct acttctgggg
    17581 aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct tttgcttctt
    17641 cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac
    17701 gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact gataataatt
    17761 taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa tcgatgaatt
    17821 cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc agttctactt
    17881 tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca cggcgttcga
    17941 tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc aaatcttctg
    18001 cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc
    18061 caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc aaatgagctg
    18121 ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc tcaatacttg
    18181 catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta ccagctaaag
    18241 ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt gttccagact
    18301 caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt tcctccttga
    18361 aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc atctcttgaa
    18421 aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc
    18481 tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc
    18541 tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga agactctcaa
    18601 attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat aaatagaaaa
    18661 atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat gttccttttt
    18721 tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag
    18781 cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct tctactaatt
    18841 cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt ttcaactcga
    18901 ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg atcttttttt
    18961 gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac agctccgaat
    19021 aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat aaagatgcca
    19081 atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac tgcttttgct
    19141 acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc agctgtagaa
    19201 ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc gtcattcacg
    19261 ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa aatggtcagt
    19321 ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt
    19381 tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc aagttttctt
    19441 agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag gatcgttcct
    19501 gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat ttcttcagct
    19561 gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc ttggattcct
    19621 tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt cacgttgttc
    19681 actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc atacagaagt
    19741 agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa tatccctttg
    19801 gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt
    19861 gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc aactaaagca
    19921 atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc agcaaatcgt
    19981 aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat caacgtgaaa
    20041 gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt tgattcttcg
    20101 actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc gatcatttgg
    20161 atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt cttggtttcc
    20221 gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt ggtcactcga
    20281 ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat atttatttca
    20341 tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct tggggtgtta
    20401 tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc tagactatct
    20461 ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac ggctaatgtt
    20521 gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg taacgtcttg
    20581 atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac aatcgcaaat
    20641 gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc aataagcaaa
    20701 gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg tgacttagag
    20761 aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt gactagcact
    20821 ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc caaaattcat
    20881 cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca gttaaaaagt
    20941 accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg aaggagcaaa
    21001 catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat cgaactaatc
    21061 cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga aatgaatcat
    21121 tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc tttttatgaa
    21181 tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca tactcttgaa
    21241 gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta ttttgcccgt
    21301 atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc ttctttaaga
    21361 gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa tctttattat
    21421 gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga tgtcgatctg
    21481 cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt agatacgtcg
    21541 gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag caaccatatg
    21601 aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga cacaacaagt
    21661 aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt gcttcaacgt
    21721 attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt tcttctattt
    21781 tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt aggtgaaagc
    21841 ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga gttttcttta
    21901 ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc gatacaagtg
    21961 atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg catcacaaac
    22021 tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc aaaggaatcg
    22081 attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag actttctcta
    22141 ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa
    22201 tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga tccaaatctt
    22261 gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt
    22321 aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa tataacgaaa
    22381 caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca taaaatcagc
    22441 gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca aagagaattg
    22501 cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc agtgacggtt
    22561 acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc atcacaagtg
    22621 gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga tctaaatcaa
    22681 aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt ggaccgtgaa
    22741 gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac tgattttgct
    22801 aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg agaccttcct
    22861 ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga taccattcga
    22921 acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac acaagaagaa
    22981 tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca gctgatttta
    23041 aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt agcaaaaggt
    23101 ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc ttctcagcaa
    23161 gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac cctttttacc
    23221 tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga gaaaaaagta
    23281 ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt atttacaata
    23341 ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata taaatccaaa
    23401 tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc ttccatgttt
    23461 tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt gtcgatcaat
    23521 cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct gattggatag
    23581 ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc gatcacttta
    23641 ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg acgatgtttc
    23701 aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc aaataaccga
    23761 aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct ataaatcagc
    23821 attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt accgacatag
    23881 tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa
    23941 tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat acgttcaaaa
    24001 ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca tacttcatat
    24061 catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa agaaaataaa
    24121 tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga aaaagactga
    24181 atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga acacatgctg
    24241 tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt gatcttctgg
    24301 agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa aatcagtgat
    24361 tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac cgtaaacgat
    24421 gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca ttaggtattc
    24481 aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt caaacatgta
    24541 agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg cccgtacgtc
    24601 tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg
    24661 tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt ttgctttttc
    24721 gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat caatgacgaa
    24781 acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg cgtagatttc
    24841 aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt tctttgctac
    24901 gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat agtttcataa
    24961 cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc tttttttctt
    25021 tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg atttgcccga
    25081 aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta actaacagtt
    25141 ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct tttttttctt
    25201 ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt ttattttgtt
    25261 ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat cgttggatcc
    25321 gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa ctatcagcag
    25381 ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag atatctgtca
    25441 gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa gattcccaag
    25501 tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt ccagctattc
    25561 tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc tgatctgtga
    25621 tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc atagggtgga
    25681 catacttggc attttccttt gcataactta atcccaagat atgatttggc gaagaaaaat
    25741 caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc ggataactat
    25801 acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc tcttggacaa
    25861 agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt
    25921 cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca gctgactgaa
    25981 ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa gcttcattcg
    26041 cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc ccgctcatta
    26101 cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga tagcgatgtc
    26161 cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc atcctccttc
    26221 atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata
    26281 cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc
    26341 aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat tcttttcttg
    26401 ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag cgctttgatt
    26461 tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt
    26521 tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt agttcatctt
    26581 cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga
    26641 tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta tccccttgat
    26701 aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt acgtcaaata
    26761 aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc tgtacctctt
    26821 gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga tattgtccga
    26881 tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct tcttcatatg
    26941 ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc actttatatc
    27001 catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc aaaatatttt
    27061 ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt
    27121 ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa tccaaccatt
    27181 cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag aaatttcgtt
    27241 ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc cattttccgc
    27301 cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta tcaatgattt
    27361 cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg aaataatctg
    27421 ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt tttgaatctg
    27481 ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc tcctaatatg
    27541 agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg gataaatctg
    27601 ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc
    27661 tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc
    27721 aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag ccccagtcgt
    27781 atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc gctctacaaa
    27841 cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact ccggatcaaa
    27901 accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg cataatcatg
    27961 tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg caagagccac
    28021 ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc tcatctgtat
    28081 tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt ccatattcat
    28141 acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag cggatagaac
    28201 agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca ggtacatcta
    28261 cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata cccacaaagt
    28321 tgaccataga agtcagctca tcgattttat accattttgg caaatattct accatatccc
    28381 caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc ttcatagtat
    28441 cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat
    28501 tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc gtctgtttct
    28561 catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga cctaaattcc
    28621 ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat gttccgccca
    28681 agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga aataattgtg
    28741 aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt gcttttactt
    28801 cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca ataacacgtc
    28861 caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct gcttcttctg
    28921 caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt tctaaagctt
    28981 cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga aaaatcggct
    29041 gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt
    29101 tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt ggcgcccagc
    29161 ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa acgatgtctg
    29221 ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg acttcatcag
    29281 gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct gctttttcca
    29341 gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa gcacgattgc
    29401 cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct tcattcagct
    29461 gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct tttttaccta
    29521 agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt
    29581 catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta
    29641 catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat tttccgacat
    29701 ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt ttttctaata
    29761 gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact ggacgaattc
    29821 ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc aatgatttag
    29881 gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca aaacggtgta
    29941 gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg atcaatgcat
    30001 ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat gagacatctt
    30061 gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc ccggaagcta
    30121 aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg tttgtttgta
    30181 taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac ctcgccagcc
    30241 catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa aacgattaaa
    30301 ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac gtatccctgc
    30361 acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta tctcatcatc
    30421 tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt tcattgctcg
    30481 tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg aattattgtt
    30541 tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag taagcaactc
    30601 ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg tcaatactgc
    30661 tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt ctaccatcca
    30721 tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga aggagcacat
    30781 aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac ctctatactc
    30841 gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga ttgatcacag
    30901 aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg tttttccagt
    30961 aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc ttttttcata
    31021 atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg aataactcga
    31081 tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc agctgcttct
    31141 gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga aagcacagca
    31201 taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag ggcaccgcca
    31261 cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga catttcgagc
    31321 agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc gcaatatgca
    31381 cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg tttcatcagt
    31441 cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg gatattttcc
    31501 tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc taatcgagcg
    31561 attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca ttcctcgaaa
    31621 gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc tcttgctaac
    31681 tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg tatgcaattc
    31741 aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat cgacaaaacc
    31801 atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct tgatcgtctg
    31861 ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta gaataatatc
    31921 gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta aaacagttag
    31981 ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg ccatctgcat
    32041 tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca agacgactgg
    32101 aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga caatcgttcc
    32161 catacttccc atgataaaat taggatccat cactccaata atgaagggaa tcccctgaat
    32221 ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca tttttttaat
    32281 tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg tatcccattc
    32341 ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga ttcgaaaagc
    32401 gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa ggatatgttt
    32461 gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag caggtttgct
    32521 ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca tctatgcgcc
    32581 tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa atgctgtgct
    32641 gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt ctgcgttcgt
    32701 gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta atgcttcaaa
    32761 acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg gcgggatcgt
    32821 atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg gaagcagtaa
    32881 gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg cattaatccg
    32941 gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca aaggctcacc
    33001 ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt ctgttactgg
    33061 atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt cttcatccat
    33121 cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc gaacagcagc
    33181 gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt cttccagtac
    33241 tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga catgaccaaa
    33301 tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt tttccaaata
    33361 catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg
    33421 gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg cagctgcttt
    33481 cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta ctgtagataa
    33541 tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt gacgcgcatt
    33601 gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg tgatattgca
    33661 ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc ctggatgaat
    33721 tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca gatatgaatc
    33781 aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat gaagtgcttc
    33841 ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc atgcacgaat
    33901 gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca tatttcacct
    33961 atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact ttagcgattc
    34021 ctttgcctat tccggctgaa gttttcactt tcaatatctc aacttcaagg tataaggtat
    34081 ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa taagccgtct
    34141 ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc attgcttcaa
    34201 caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa aagggctcat
    34261 tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct tccactcgat
    34321 caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt atgttcattg
    34381 ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc tgtccatatt
    34441 cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc tcactagtta
    34501 tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg gcaacttgat
    34561 cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg attcccacga
    34621 ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc attatttctg
    34681 gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc aacgtttcgt
    34741 ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac aattcaaatt
    34801 gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg actaattctc
    34861 tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa ccgcattatg
    34921 tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt
    34981 tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg taggtgggat
    35041 aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa
    35101 taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat tttcacctaa
    35161 tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg ttccgtgagc
    35221 attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa gcttcattgc
    35281 tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt cacagtttgc
    35341 accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat gttctaaaga
    35401 ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat tttcgtcaaa
    35461 cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag ctgcaaatcc
    35521 agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga catcagaata
    35581 gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag cacaagctgt
    35641 tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc cagctgccat
    35701 gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt tttcatgtag
    35761 acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga tgatgacgcc
    35821 aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg cttctaaagc
    35881 agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt taataaaata
    35941 tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt ctgaagcatc
    36001 aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca aaaagctgtc
    36061 tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc gattcatttg
    36121 tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga tgacttgtcc
    36181 ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc
    36241 gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg ctaaaacttc
    36301 cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc ctctagttgc
    36361 agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag ctgcataatt
    36421 tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga tggccccttc
    36481 tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt
    36541 gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt tgtcatttgt
    36601 gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg cttctttgat
    36661 catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc attttacacc
    36721 gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat tcaaaatgat
    36781 atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc ttgtactacc
    36841 tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag tattgctatt
    36901 gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt atcgattttt
    36961 ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc gatgccgatt
    37021 gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt
    37081 agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat gataggaaaa
    37141 gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc tgccggttct
    37201 aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt gaaccctttt
    37261 tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat cacgatctgc
    37321 tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc atgacagctt
    37381 tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct tccagctggg
    37441 gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc agtgaaggat
    37501 agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa agctgcatct
    37561 gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt tagaatagct
    37621 ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag ttcagccata
    37681 tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc ttcttcatat
    37741 aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa tgctgttttc
    37801 atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa aaaggccgag
    37861 acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt atgcagtttg
    37921 tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg tttcgatttt
    37981 cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc tgtcagcttc
    38041 taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc ctaattcttc
    38101 tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg atattgtttg
    38161 ctttttattt ttataattta acagtctaca actccatgag aatacttccc catgttaaac
    38221 ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc tttccactag
    38281 aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca tatttatcca
    38341 tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc tcaataattc
    38401 tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg acttcttgga
    38461 tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc atttgcaaat
    38521 aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg tagccagcgg
    38581 tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat tttttctgtg
    38641 gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca gtcgtacgat
    38701 cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc ttagttgagc
    38761 cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag cacgcagcac
    38821 tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt gctgctacag
    38881 atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa tctttagggt
    38941 ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca gaagtctcct
    39001 gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat tcatcagagg
    39061 tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg acgctggctg
    39121 ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct tgcaagaagt
    39181 cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc attccgtcta
    39241 agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt aactttcctt
    39301 ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata cgttcgacat
    39361 agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta attgatagtt
    39421 ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg atcgtgtgca
    39481 tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct tcgattgtca
    39541 aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct ggttccatgt
    39601 tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact atatcttaac
    39661 ttttttcaaa atgcaacctt aaaagatcat ttttttacta tatgagcttt ttttaagatt
    39721 ttactcattt aaaacaactg ataaatcagc atttataatt tcttttatta gaaaaatcat
    39781 ctatctcaca tattttgatt atcaaagtat tttctgtgaa cagaactata gaattcccta
    39841 aaacttgtcc attatttcaa aatttgttat agtcttttta ttcaaagcgt aaaaggagat
    39901 cctatgttaa aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa
    39961 gaccgagatg gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc
    40021 agctattttc atcagaataa acagcattca aaaaaagtat tacgtatgat tcatcatata
    40081 acagattttg atttcgtcgt agtagacacg gaattagatg ctttattgct cgagtgtcaa
    40141 ttgatccaac attaccgtcc tttttataac c
//
LOCUS       DACEZN010000008        40070 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEZN010000008 DACEZN010000000
VERSION     DACEZN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.008"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(208..1626)
                     /gene="nhaC"
                     /locus_tag="IXH60_000302"
     CDS             complement(208..1626)
                     /gene="nhaC"
                     /locus_tag="IXH60_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1989273.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(2029..3252)
                     /locus_tag="IXH60_000303"
     CDS             complement(2029..3252)
                     /locus_tag="IXH60_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1989274.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3283..4122)
                     /locus_tag="IXH60_000304"
     CDS             complement(3283..4122)
                     /locus_tag="IXH60_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1989275.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4139..4819)
                     /locus_tag="IXH60_000305"
     CDS             complement(4139..4819)
                     /locus_tag="IXH60_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989276.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4821..5894)
                     /locus_tag="IXH60_000306"
     CDS             complement(4821..5894)
                     /locus_tag="IXH60_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1989277.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6541..7212)
                     /locus_tag="IXH60_000307"
     CDS             complement(6541..7212)
                     /locus_tag="IXH60_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1989278.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7299..9977)
                     /gene="mgtA"
                     /locus_tag="IXH60_000308"
     CDS             complement(7299..9977)
                     /gene="mgtA"
                     /locus_tag="IXH60_000308"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1989279.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10636..11073
                     /gene="ndk"
                     /locus_tag="IXH60_000309"
     CDS             10636..11073
                     /gene="ndk"
                     /locus_tag="IXH60_000309"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1989280.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11095..11961)
                     /locus_tag="IXH60_000310"
     CDS             complement(11095..11961)
                     /locus_tag="IXH60_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1989281.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12121..14214)
                     /locus_tag="IXH60_000311"
     CDS             complement(12121..14214)
                     /locus_tag="IXH60_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1989282.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(14963..15586)
                     /locus_tag="IXH60_000312"
     CDS             complement(14963..15586)
                     /locus_tag="IXH60_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989283.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(15745..17142)
                     /locus_tag="IXH60_000313"
     CDS             complement(15745..17142)
                     /locus_tag="IXH60_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1989284.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(17174..18145)
                     /locus_tag="IXH60_000314"
     CDS             complement(17174..18145)
                     /locus_tag="IXH60_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1989285.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            18519..18944
                     /locus_tag="IXH60_000315"
     CDS             18519..18944
                     /locus_tag="IXH60_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989286.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(19432..20133)
                     /locus_tag="IXH60_000316"
     CDS             complement(19432..20133)
                     /locus_tag="IXH60_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1989287.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(20149..20589)
                     /locus_tag="IXH60_000317"
     CDS             complement(20149..20589)
                     /locus_tag="IXH60_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1989288.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(20589..21164)
                     /locus_tag="IXH60_000318"
     CDS             complement(20589..21164)
                     /locus_tag="IXH60_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1989289.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(22012..22491)
                     /locus_tag="IXH60_000319"
                     /pseudo
     CDS             complement(22012..22491)
                     /locus_tag="IXH60_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(22650..23351)
                     /locus_tag="IXH60_000320"
     CDS             complement(22650..23351)
                     /locus_tag="IXH60_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1989290.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(23459..24868)
                     /locus_tag="IXH60_000321"
     CDS             complement(23459..24868)
                     /locus_tag="IXH60_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1989291.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(24916..26781)
                     /locus_tag="IXH60_000322"
     CDS             complement(24916..26781)
                     /locus_tag="IXH60_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1989292.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(26902..27753)
                     /locus_tag="IXH60_000323"
     CDS             complement(26902..27753)
                     /locus_tag="IXH60_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1989293.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(28063..28776)
                     /locus_tag="IXH60_000324"
     CDS             complement(28063..28776)
                     /locus_tag="IXH60_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1989294.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(28844..29170)
                     /locus_tag="IXH60_000325"
     CDS             complement(28844..29170)
                     /locus_tag="IXH60_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1989295.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELVDVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(29293..29520)
                     /locus_tag="IXH60_000326"
     CDS             complement(29293..29520)
                     /locus_tag="IXH60_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1989296.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(29842..31095)
                     /locus_tag="IXH60_000327"
     CDS             complement(29842..31095)
                     /locus_tag="IXH60_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1989297.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            31156..31896
                     /locus_tag="IXH60_000328"
     CDS             31156..31896
                     /locus_tag="IXH60_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1989298.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(31982..32740)
                     /locus_tag="IXH60_000329"
     CDS             complement(31982..32740)
                     /locus_tag="IXH60_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1989299.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(32737..33765)
                     /locus_tag="IXH60_000330"
                     /pseudo
     CDS             complement(32737..33765)
                     /locus_tag="IXH60_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(33785..36952)
                     /locus_tag="IXH60_000331"
     CDS             complement(33785..36952)
                     /locus_tag="IXH60_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989300.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(37176..38060)
                     /locus_tag="IXH60_000332"
     CDS             complement(37176..38060)
                     /locus_tag="IXH60_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1989301.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(38155..38592)
                     /locus_tag="IXH60_000333"
     CDS             complement(38155..38592)
                     /locus_tag="IXH60_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989302.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(38809..39720)
                     /locus_tag="IXH60_000334"
     CDS             complement(38809..39720)
                     /locus_tag="IXH60_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1989303.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT        11452 a         8494 c         6690 g        13434 t
ORIGIN      
        1 ctaataccga gggtcagctg attgaacacc gtttatcgag aggttgtgaa aaagtcgctt
       61 agtttcgcgt attaagaaaa attttgtgaa atggctttcc acatttttgt ataaatttgg
      121 cttaataccg agaaactggc ttttgaatac cgtttattcg taaaagggat tgtgacaaaa
      181 gtcttgtcac aatccctttt tatgttttta ttgttctaat tgcgctgtct tgcttactgc
      241 ttcttgcggt ttcacaaatt tttttgagat tccccaacct gtgaaggcac tgaccatcga
      301 aaacacagga gaaagcaaac tgaaaaatac gaatggcaag taagtgagtg taggaatacc
      361 aaatgtactt gctacgaaac ttccggcaat tccccaaggc actaagtagt tgattactgt
      421 tccaccatct tctagcgttc tacctaatac agttggatca aatcctgctt tttcataagt
      481 ttctttaaat gcattaccag gcagaataac agataggaat tgttcaccaa taaaaatatt
      541 tactccgata cttgtcatca atgttgtaaa aatcaaattt ggtgtgttcg tcatctttct
      601 tgatactcgt tccatcacgg ttgagatcaa gccaaattcg atcaacaatc cacctaatga
      661 caaagttaga ataatcaatg acacagtagg catcatactt tcgattcctc cgcggctaag
      721 cagtgaatcg atttgttggt ttccagtatt tgaaacaaac cctgattcga tgaccccagc
      781 caacttcgtt aaggaagttt gtgggttttg aataaaaatc attcctactg ctacagcgat
      841 attcaacaaa atcgttaaaa ttgccggcac ctttttccat gcacacagta acattaaaaa
      901 taaaggaatc agtgaccaga ttgaaatagt aaagtttgct tctagaacat cagtcacttc
      961 acgaatagat gaaagacttg cggctttact tgtgtgtcct aaaatcatga ataaaaagaa
     1021 agaaacaaca aatgccggga cagttgacca catcatatgc ttgatgtgtt tgaacaaatc
     1081 ggcatcaaca atagctgctg ctagatttgt tgattcggat aatggagaca tcttatctcc
     1141 aaaaacggca cctgaaacaa tagctccaac aaccagcgct ggatttaagc ctagtgttgt
     1201 cccaataccg aaaaatgcaa tcccaatcgt tgacataaca gtaaaggcac tccctacagc
     1261 gctacctacg attgcacaca caacaaatac agaagggaca aaccactgga cactgatcaa
     1321 tttgaaccca atgaccatta gagttggaat gatccctgct tgtatccata cagcaatcaa
     1381 tgaaccgact aaaataaaaa taaagattgg gatgattcct ggtttgatgc cattgatgat
     1441 tccttcgtgc agccaatctg tcgggtatct ttttatcact gcatacaaca tgaccattcc
     1501 gatcgctgct aagatcgtga tattaggtga gacctttaat ccgacaacgc caatcgcaat
     1561 gcctaaaaca ataaccatta atacaagtgt tgcttctaaa aaacttggtg ttcctttttt
     1621 tcccatgata atctctcctt ttttagttgt tcttatgttc taaaaaagtt aagatatcgg
     1681 attactccgc cgcaattcat ccgcgattcc tcctttataa aaataaaaag acctgtttta
     1741 aactaaggac gaatgactcc gcggtaccac cttaattaaa aacagatctg tttttcactt
     1801 tcaaactttt attcaaatgt atggactttc catgtaattc gtcaattagg cttgatcggt
     1861 tcacagcgac caccgacttt ctgaacaaaa cacctaaccg ctactgcgtg aaaagttgac
     1921 ttgtacgaag tttaccttcc ataagatcat ttgtcaatga aaaagaaaat attttttcat
     1981 ttttctgaaa attattttta taactttcac acacaagttt attttttact agaaagtagc
     2041 gccctcttgt ttttttcttg ctactatttc ttgatgttct tgcttgattc tttctaatag
     2101 ctttggctct ttcaacagat caagagctgt attggcgagt agttctgctg caatcgcaat
     2161 agaagcaagg cctttgtcac ttttagcagc agctttgaat tcttctgtgt gtccggcaat
     2221 atatgtctca gagattgcta ccgtaggctg gattgttggt atgacttggc tgacatttcc
     2281 tacatcagaa gaaccgagac ttgttctttg ttccgtttct atttcttcat ctggaacacc
     2341 tgcttctttt gcatgctgga agaataagtc atcaaaagaa ggagtaacga tgacatcatc
     2401 aactgcattt tggaacaatc caaactcaaa agtcgtgccc gtacttatcg cagcacctcg
     2461 tacgatattt tccacttttt catatacttt atctaatgtt tgccgatttc ctgcacgtaa
     2521 atagaaacgt ccagcagcgt aatctggaat gacgttggca gctactcctc cgtctgtaat
     2581 gatcccatgg atattgacat cttttggcaa ttgcaagcgt agagcgttga ttccattaaa
     2641 cacttggatc aatgcttcca aagcatttac tcctttttca ggtgctgctg ctgcatggga
     2701 agcgacaccg taaaatttga tgtcgactgg atcattcgct aaagattctg tcgttttccc
     2761 ataacgaaaa gctggatgga cgcataacgc tacatcgaca tcctcaaaaa aaccttctcg
     2821 aacaaagctg cctttagctg atccgttctc gcccccttct tcccctgggg tgccatatac
     2881 acggatttct ccaccagttt catcaatgaa ttgcttcact atgctggcgg ctaatacgga
     2941 gtaagtacca aacaaattat gcccgcatgc atgaccaata cctggtaaag catcgaattc
     3001 tgccaaaaag gcaattgtgg gaccgggttt attggaaata taacgtgcat caaaggctgt
     3061 tcggtgtcca gctacttctt tttctaccca aaacctttct ttttctaatt gtttaatcaa
     3121 cgctgctgag gaataaaact catagttact tacttcagga tgttcgtgga tatctagagc
     3181 aagtgcttga tattctggta aacgatattg gattagatcc ttcaattggt ttgttgagat
     3241 tgttgttgtc ataaaaaatt cctcctatgc tagtcatttt tcttattttc tcccgaatgt
     3301 ttcccatgca ggcacgcttg atccttttga tgttttctcg attactttga ctgtgtcttc
     3361 tgtttgataa gcgtctacta ttttttgata cgttttattg ttttcatctt cttttcttgc
     3421 aacgatgata ttcacataag gacgtgcatg gtcatccact ggttctaaga agatggcatc
     3481 agaagcagga ttaaatccag catctacagc tacaccgcta ttgatcactg aaatgtctac
     3541 atctgacaaa gcacgtgctg tttgggaagc atctaattca gaaatgtcta gattttttct
     3601 attctcggta atatcagaaa cagtgggcgc ttgtcctttt gccttatcca ctttgatcaa
     3661 tccagcagtt tgcaataaca gaagagctct gccgccattt gtaacatcat tagggattgc
     3721 tacttttgct ccgtctttga tatcagctac atccttcaaa gtgttagaat aaattcccag
     3781 cggtgcattg acagtatttc cgatcgatac taaatcagtt ccgtgttctt tattgtaatt
     3841 gtctaaaaag aattgatgtt ggaaagaatt caaatcaatc gatccatctg ctaaagccac
     3901 atttggttga ttgtaatctg taaatttcac gtattccaaa ttaatgttct cctttttcaa
     3961 acggtctttc acgttgtccc atacatctgt atcttctcca ataatcccta gttttacgat
     4021 agtttctttt ttgtcagttg tatctgcttt tccttggcta caccctgcca accaaataat
     4081 tgctacaaat gtaatgacta caaaacgaat aatttttttc attttttctc ctccactttc
     4141 aatgtgtaat ttttttaatc agttgattac tgataaattg acttaaaaaa actaagatta
     4201 gaatcaataa tgtagccaca atcgttacat ctgtttggaa tcgattatac cctctcgata
     4261 tagcaagatt ccctaatccg cctgcaccta ctgctccagc catggtagtc agtccgatca
     4321 cgttgatgat agtcaacgcc gagacccgaa tgataccagg caaaccttct aacaaataca
     4381 cacgtaaaat gataccgatt ggactggttc ccatcgcttc tgccgcttcg atcaccccag
     4441 gatcaacttc tagcagtgcg ttctcgattt gacgagcaaa aaacggaaca actccaaaaa
     4501 tcaatggcac taaagctgct ttcgcaccaa tcgtcgttcc aactaagaaa cgagtagtca
     4561 atgctaaaag agaaagcaag ataatgaaag ggatggaacg aatgatattg atcagcttat
     4621 caagtatttc aaatagcaga cggttagcta gtaatccatt agggcgtgtc actactaacg
     4681 tcacacctaa gatgattcca atcatcccgc caatgaatgc tgtccaaaaa gacatatata
     4741 gtgtttgctt cgtcgcttcg acaaattcat ccggaatcaa ataaacattg ggcaaatatg
     4801 ttttaaagaa atcagtcatt ttatcttcct ccttcgatca ctttgatacg actatctaaa
     4861 tcgttttttt gtaaaatatt tatttttaca ccagatttga tcaaataatc caatgctttt
     4921 ttccgctgtt tggtttctcc agctaaagta acgatcaaac tccctaacgg cgtttcttgt
     4981 aataactcta cattgccata taaaatattc gctgttactt gatatttgct gtacagatga
     5041 gcgattagag gttcgttcgt ctgatttcca atataactta attccaccag ccatttattg
     5101 gcgatctgat cggcaaaagc agaatgggca atgatcgttt ctaatgcttg gtcaagatga
     5161 gtagctgttc gaataaaatc tttggtcaat tcttcttcag gattgctgaa gatctggatg
     5221 ctgctacctt tttcaacgat tcttccatct tccataaccg ctaccttgtt gcagatttct
     5281 ttgacaactt gcatctcatg agtgatcagg acgattgtta aacctaactg gcgattcaat
     5341 ttttttaaca atgcaaggat ctgaagggtc gttttcggat caagtgcact ggttgcttca
     5401 tcacataata ataccttagg ttcattcgcc aaagctcgtg cgattgctac tcgttgtttt
     5461 tgtccccccg acagttgttt aggataagca gatgcttttt cttccaaacc gacaagttcc
     5521 agtagttcat tcactttttg acgacgttcc tgtttcgatt ttccagaata ctttaaagaa
     5581 aaatcaacgt tgtcaaaaat agttcggctt tccattagat tgaaatgctg aaagatcata
     5641 ccgatggcct ttctttcttt tcgcaattct ttgggagaga gctgactcag attcgtctga
     5701 ttgacaatga cttctcctgc tgttggtttc tgcagcaaat tcatcacacg aactaacgta
     5761 cttttccctg cacctgagta accgacgatt ccataaacat cgcctttttc aatcgttaaa
     5821 tctacagaat ctactgcctg tatctctttt tctttttgtt tgaaacatac agaaacatct
     5881 cgcaattcaa tcattaaaat tcctccttat ctaaaaaaag cgtcctcaag aaaatgaaaa
     5941 tgtcattttc ttgaggacgc ttttttgcgt gttaccacct catgttcaag ctgtgttcac
     6001 acacaactcc tcttcaagta cgatcatact ctagcaagat atcggtcgca gccgtcatcg
     6061 ccttacatct atgctcggga tgaattgctc gatggccatt ttccaatagt cgttccttgc
     6121 ttcttctcag ctaccgaagc tctctgtcaa aggttcttct atcgtactta ccatttcaat
     6181 gcaaataata ttcaaaaaaa aagtcctttt actaacataa actatgtcag taaaaggacg
     6241 acgtatcgcg gtaccacctt ttttcggaag tttccttccc tcaagcccaa catctcaaaa
     6301 agagagattg gcgttcgttt taacggtcga acagttccga atttgcctac ttatcgacaa
     6361 attattctcc aaggccattt tcagcttttc ttacttgact cttctcacca gccagagcct
     6421 ctctgcaaag tttccaaact tactttcctt ttcaacgaaa tcgatatttt gttgttggga
     6481 ttaatctaca tgaggaaaac tgaaaagtca agtacgaaat tcaaattatt ttttctatat
     6541 ttatttttct gtttttttat ttaacatttc aataacagtt cgaagatctt ttccttccgc
     6601 aagaagtttc aatttttcct tttcttcgac ggtataattt cggacaaaac tggaatcagg
     6661 tatcaaaaca acgcctaatc ctgctcgtga agcagctgtt gctcctacta aagaatcttc
     6721 cactgcaaag gctgaactag cttctaatcc tgttcgttcc aaagctgtca gataaggaat
     6781 cggtgaaggc ttgttctccg acacttcgtc tccgaatgta aacgtgtcca agtaatcagc
     6841 taatttaaag tgatccaaga tatctgttgc tcttttcgta acagtggtcg tagcaatgcc
     6901 tagtttcata tgacgttctt ttaagtctgc caatatttct tttgcatatg gttttaagtg
     6961 gatggctccg cttgtcagct gttgataaaa atattcttca cgcttcgctc ggacttcctg
     7021 tactttttca gggctacctg ctgctttcac taaaatatca aaactttgtt tccagttttt
     7081 tccgccccaa gcgtcaacga tttcttttgg gatatggata tcaaattgct gaaatcccca
     7141 aagccagcca tcacgatacg ttttttctgt gtcaactaac aaaccatcta aatcaaatat
     7201 tgcacctttc atctatacat taatcctttc caaaacaata cacatagttt agcacgactg
     7261 gtccaaaaag aacagaagca aagaaccagc cttctcgttt ataagagttc ttggaatcga
     7321 cggatataga ttttcttcac agctgtcaca agcgccagat aagcgatgac tgtagcaaat
     7381 aaccataacc agtaattgcc aggaagtggc atcaagccta attcagcacc aaaacttgta
     7441 aaaggaagga cagaaccgac aataattcct aatgtagtga taacaaacat ggcaaatgtt
     7501 gcgttgcttt ggataaatgg tactttcggt gtacgcagtg catggagcac taaggtttgt
     7561 gtccatagtg attcgacaaa ccatcctgca tggaataatg cgatgaaaat cactcgctgt
     7621 gaactatcca atgtatggaa acttccgccc actacagcag gacaaataac aaaatacatc
     7681 aacaagtaag tagtaatatc aaatatcgaa cttgttggac caaagtaaac catgaattcc
     7741 ccaatactag atgcattcca tttttttggc tcgtccaaat attctttatc catccgatcc
     7801 catggcaagg aaatgcagga aacatcatag atcagattca aaaacaaaag ttgtaaaggc
     7861 agcattggta aaaatggcag gaacgtactt gcaatcagca cggaaaacat attgccaaaa
     7921 ttagaacttg ctgtagcctt gatatatttc atgatattcc caaaagtttc tcgtccagaa
     7981 agaaggcctc tttccaaaat cgtcaaatct ttttctaaaa ggatcacgtc tgcagattct
     8041 ttcgcaatat ccacagctgt atctacagaa ataccgatat cagccacttt cattgcagga
     8101 gcgtcattga taccatctcc taaaaatccg acagtatgac catttttacg taaagctgtc
     8161 gtcaaacgtg ctttttgctg aggattcaat tttacgaata cttcatggtc ttcagctact
     8221 tgtgctaatt catgatcact catttccgag attttctcac cggtaaccaa ttcattgatc
     8281 tctagaccta cttgacggca cactgaacgt gtaactaatt cgttgtcccc agtcagaact
     8341 tttacagcta caccatgttc tttcagagct ttcaacgctt gtttcgttgt ttcttttggt
     8401 ggatcaagga aagcaagata accaatcaaa accatgtctg actcatcttt gactgaaaat
     8461 tctccaacga cgcttggatt ggtcttttgt gcgacaccaa tcactcgtaa gccatcttca
     8521 ttaagtgact gtacttttgc aagtacggct tcttttcgtt cttttgttaa tggtgtaaca
     8581 gtaccgccaa gatcaataaa tttagaaatg ttcagcattt cttcaatcgc gccttttgtg
     8641 atcatttgtg ttttaccagc tggatcagca accacaacac tcatacgacg acgttcaaaa
     8701 tcaaaaggaa tttcatccac tttttgataa ttgatcttat ctgtatctaa agtttctgtt
     8761 gcttcatcaa tgatagccac atctaacaaa tttttcagcc ctgtttgata gtagctattt
     8821 agatacgcat ggcgcaaaac acgatcatct tcatttcctt cacaatccat atgatattca
     8881 agaatgattt tatcttgtgt caatgtcccc gttttatccg tacataacac atcgattgca
     8941 ccaaagttct ggatggcatt caaatttttg atcactgttc cttttttggc catcacactt
     9001 gctcctttga ccaaattcgt cgtaacgatc attggaagca tttcaggtgt tagaccaact
     9061 gctacagata gtccaaaaag aaaagcttct agccaatccc cttttgtaag cccattgatg
     9121 acgataactg ctggagccat caatgacata aatttgatca gtagcatgga tgttttatgg
     9181 atgcctaatt caaagcttga ttgaattggt ttttcagata gcgtttctgc tacatgacca
     9241 aatagtgttt catttccaac agaaacaatg attccttccg cacttccact gatgacatta
     9301 cttcccataa agacaagatt ttcgtaactt gtttcgcttt gctgatcgtc ttcacatact
     9361 ttcgcatgct tttctactgg atagctttcc cctgtcaatg ctgcttggga aatgaataaa
     9421 tcctttgcgg acaataaacg catatccgct ggtatcatat cccctgctga aagccgaaca
     9481 agatctccac agacgatttc ttccatagga atctctgtgt actttccatt acgttttaca
     9541 gctgccgtta ctttcacaag tgatttcagt ttttcagccg cttgattgga acgtacggac
     9601 tgaatcaacg tcattgtccc gctgacgata accatagtga agataatggc tacaccgaaa
     9661 agatctttat ctccaggagc tgctaataca tattccgtga tgaatgaaat aattcctaat
     9721 gcgatcaaca ctaatgtaaa tggcgtgatg tatgctttca atatttctac aatgaatggt
     9781 gtttttttgc catatgaaat cgcattttca ccgtaatttt ctcttgaacg ttcgatttgt
     9841 tcttctgtca atccatttgc tgatgcatgg aatagttcaa ataattcttg tttcgttttt
     9901 ccagcaaaag ctgcatagat tttttgtgta tctttctttt tttccatctc aatctctcct
     9961 ttgtgtccaa aagacagctg ttctcgctgc ttttggtctt tagctattca tcggatcgat
    10021 agagaaaaaa ctgaaagggg ctgagaacag atagataata gccctggcct ttcctctaga
    10081 tccttgtttt tcgaatgact gtcatccatt tgttctcatc tcctttacta agtaatttta
    10141 attcatgact tccaatcatt tttcgaatag ttatttttcc aattagacca cctccttttt
    10201 atttttcacc gatggggatt tgcggcaaca aaaaaacctc tcgccaaata ctcagcgaaa
    10261 ggtttgaaaa agcatattaa aaaaagcttc tcagatctca tcgttgagtt ttgacactgt
    10321 acaacgtaaa aagcagatac ctgcttttag ctaccttatg aaaagcctta tctcgacaat
    10381 tcctgttcta cccattggcg tctctcgacg tttttgggca gtagcctatg tttgtatagg
    10441 agcctcacct aacaaatctt attcggtttt ccttcgttaa tataacaact aaaaaagagg
    10501 atgtcaaatc aattttccat gtttttcata ctttttttaa aatacacacg ttatattcct
    10561 gtctcacgcg gttttatttg tctcattttc cacctcccgc tataatagaa atatagtagg
    10621 aaacgaggtg ctgttatgga aagaacactt gttattatca agccagacgg cgtgagacgc
    10681 catttagtcg gaagcatcat ccaacgtttt gaagcaaagg gactggccat tgctgaaatg
    10741 aagtttgaaa ctatgactcc tgaattagca aaagaacatt atcagcactt gactgagcgc
    10801 agtttttttg atgaactgat cgattacatg acttccggtc ctgtcgttta tcttgtgctg
    10861 gtcggcgagg aagtgatcga tatcgtccgt aagatggttg gagcaacaaa agctgccgat
    10921 gctgttccag gtacgatccg aggagactac gcgctgccgg ggactgaaaa catcattcac
    10981 gcttctgatt cccgtgatgc tgcggtaaaa gagatcgccc gctttttccc gccattcgat
    11041 ataaacaaac cgtctgaaaa agcggcgaag tgatacaaat tctaaactgc tggtctagtt
    11101 gctgggttca gcaatggcca gcagtttttt ctctaaggac ctccgttgat tctttcctaa
    11161 catatattcg agcactgtga agaattcttg gtagtctttc gtcttttctt cccctaaagc
    11221 agcagaaatc aatctttgat aataatctgc catcaatgtt tcatagatca ctgtacttct
    11281 ttctttatgt gataagctcc gtttcatatc ttcattcaca ctacgtgcct ttttcaaccg
    11341 accattttct atataaaacg aagtcaggtt cattaaaaaa gtaaagagat actgctcatg
    11401 attgttcaac tgtgctaatc gtcgcattct ttttttgatt ttcaaataat tgttctcaat
    11461 gaaccgatca ctgaatatcc aaggaaaatc agctagaata gatagttcaa attgaccata
    11521 gtctggtatc tgcatcaagt attcttcaat gattcggcat tcataacgta aaaattcaaa
    11581 ttctgtctca gatacttctg ttttttcgac catgtgaaga ttcaaaacca aataatacag
    11641 atcttctgtt ttcttaaaat gcctacggtt attgctgatc tctttataag gatcaaagct
    11701 gtcttcttgt acccgatcta gcattcgtct ttttttcttt aactttattg gttcatccag
    11761 ctggtttcgc atccattcat attctgcaag tgtcatgttc attcgctcta aaaatgatct
    11821 caatgttaca aatgacattt ttttcactga atttgtctca atcccgacta gagaagtctt
    11881 aaaacagatt ccttccgcta attgggactg tgtcaacttc ttgttgactc gaagtttctt
    11941 gatcaattca ccatcataca taatagttct cctttttaat ttaaattttt ccacattcaa
    12001 taaatagata cgttaaaaag gaaaaataca cgaaaaaaat aaaaaaatgt ttttttcata
    12061 aaaaatacca accttcaagc attagaatgt tgtctaacta ctgaaggttg gtttgattgt
    12121 ttattgatta tttttttgct gttcaacgtc tgttcttgtt actggatata agttggttgt
    12181 tgttttatta ccattttttg aaaagacact tgtcgaatgt tcgccttttt gcttttcgat
    12241 tgcttcatta cgttccgctt catttttata gtcatatttt ttcggatcaa tcgtctcaaa
    12301 tccgtttggc acataaaacc gcaagagatt ttcctgattc agcttatctg aaagacttaa
    12361 cgcagtcttg actttttcct gatctttgga gatttcctca tcgattcctg ctgctttcgt
    12421 atcgatgatt tctcctgtct gattattgta agcttttcct cctaggacgg tgtatttggg
    12481 tgtcacaaag ttcccatttc gaaaagcaac tacttgatca tgctgagaag ataataagtc
    12541 tgtaccaaaa tgaagataat ctttatcatc gattcccaat agatgtagta atgtcggcaa
    12601 aacatcgatt tctccaccgt attcatgatt gactgtccct ttagtataac caggcatatg
    12661 gatcatcaat ggcactcgtt gcatctgtgc gttgtcaaat tcatcccatg tatctggatc
    12721 tttaccaaga gcgcttgcta aatctttatt atctgtattc gagatcccaa aatgatcccc
    12781 gtaaatcaca aagattgaat tttggtaaat accagatttt ttcaaatacg taaaaaattg
    12841 ttccaatgat tgatccagat aatgtgccgt tcgaacataa ttatctactg tgctattgcc
    12901 agtattcaaa gatggaaagt cgaaattctt gtcatccgta taataaggcg tgtggttggt
    12961 tacggaaaga aatttcgtgt aaaacggctg ttgtagatgc tcaagatatt gggcagattc
    13021 tctgaataaa tctttatcta atatcccata acctaacgtt tcgtcggatt catcaaaata
    13081 agatcgatca aagaaattgt cataacctaa gtttttatat acatgatctc tattccaaaa
    13141 actagctaca ttcccatgga aaacagcgct tgtatagcct tgcttttgtc caaggatcgc
    13201 tggtgcggct tgaaagacat tgtctgaccc aagttctgtg aataatgagc cttgaggtaa
    13261 accaaatgtg ccagtttcca acatattttc agcatcactt gtctttccct gtcctacttc
    13321 atgaaagaaa ttgtcaaagc tgatcgttgc ttgattctgg aaaatactat ttaaaaacgg
    13381 tgtgacttct tgtccgtcta ccttcatatt aatcaaaaac tgctggaaac tttctaaatg
    13441 gagaacgatc acatttttcc ctttagcgat cccaaatgtt tcgggatttg gctctgcata
    13501 atgtttcttt gtataatcta aaacttcatc aatcccatta ctgcttgcat gcgcgcgaac
    13561 actgctagtc attcctgttt tgattccgtc ataaatagta tacgcatcga ttcctagata
    13621 tttgacaata tatgaacggt caaatgttcg ctgcagcaat tgtggacggt tggcttcgct
    13681 cagcgctaga tttcctgaaa aaataagccc tgaaagacag gtaacagcga tagccatcgg
    13741 tttgccgaca gggttgcttt tgataggtat tttcttgacc ttcaaccaaa ccagtagccc
    13801 aatgaacaca gcgatatcca gccagtagat gacatcatgg ggcttcatca aagaaaagga
    13861 gcttcctgac aatccttgcg acactttcga aaagcctaat acagatttga ctgtaatgaa
    13921 atctgtgaat tcccgataaa aaataatatt caagtacaac aaaagcgtat ttaaaatatc
    13981 aatgatcaaa agaatagcta acgctggctt tggcttttta atatacaaag aaaggccaaa
    14041 aaacaataag gtagtcgcaa ttgggttgat ccacaagata aagtactgga tcgtaccttc
    14101 cacacctaat gaaaaatctg catagtaggc aatgatcgtc ttcagccaga aaaagaaaac
    14161 agctgtcaaa acgattgcta aacgattttg gcacaatttt ttaagttgtg tcacgtatat
    14221 cacttctctc tattcacatt ttttcttatt ttgctcctga aaaatgaaaa tttcctaaaa
    14281 aagttgttat cttaagattt ttttattctt tctaaagatt attccgtcca cttttcacag
    14341 cataaaaccg ctgcgatgta agcgattaaa ctaatgaaga gataaaagtc ttgtggtgac
    14401 cgcttcactc cacggtattc aaaagccagt ttctcggtat tcgtacaaaa aaagaggctg
    14461 tgacaaaatt cttgtcgcag ctccttattt cttgatgcag gacagctttt tcacaacttc
    14521 ttccttaaac ggtgttctcc tgccaactcc tcggccacaa gtcacaatat ttataaaaca
    14581 tgagaagctg ttttttgaaa ttgttccttg tggctcagag ttaaacggca ggctcaagac
    14641 ctcttgatac acggtgttca atcagctgac cctcggtatt agattatgtc aggacgctaa
    14701 ttttacaacc tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    14761 aaacgaaaaa tataaggagc tattttttga aattgctcct tgtggctcag agttaaacgg
    14821 caggctcaag acctcttgat acacggtgtt caatcagctg accttcggta ttagctcaaa
    14881 aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaaacg
    14941 ctgatttcac aacctctgtt tttcattgtc cataggattg atattgtcct tgtgatgact
    15001 cattcgtaat aattgaggct tgattttctt cacttcccgt tatcgtttgt ccactgccag
    15061 aatctgaagg acttgttcct gtcgactcgt ttttttgaga agagacagtt gttttcttga
    15121 cttgttcatt tgtctttttc cattccgcta aacgatcggc gaccactttt ttgtctactt
    15181 tttcagaata agcggtgatc acttttccat ttttcaaacg ggctactttg tcgccatcat
    15241 gagtcaaaag caaaacggtc aaatgcttgt ctttaaattg atattgtctg agagaaatcg
    15301 cactaagtac gtcatcgata ttaataaatt gattttcttc ttttcctttt ggcaaataaa
    15361 tgtccaattc gcctttttcg taattggcag ttattttata ctccgcccac caataagttg
    15421 agaattcttt tttgaaatta tctactgatt ctttgcttgc tacgattttt tctgtttctt
    15481 tctgagcaag atctgctgtc atttttcctg aaaatgctgc ggataaaata agcagtaaga
    15541 aaaaaccgaa taaccaaaag agaagaaacg gtcttttttt cctcatgctt tcctccactc
    15601 ctatttaatt ttgactattc tattatatac aaaaaatgaa atagaaagac aaaaaagttg
    15661 aaaaagatcg taaataattg aacttctttt tcacaaaatg gtaagaatgg agaatttcac
    15721 gcatttgctc tcaactattc tttcttaagc ctcaaatccg tttttatctg atacgtcttt
    15781 gaaccaatga cccgattttt tgattgttct ttttccatct tgtgctaagt ctactgaaat
    15841 aaagccatat cgattcttat atgcattggt ccacgaccag ttatccatac atgtccacat
    15901 atgatagcct aaacaattgg caccttcttg aatggcttga tggacatatt tcagatgaga
    15961 ttggacaaat tcgatccgat aatcatcgtg gatcatgcca tcttctttga taaatcgtgc
    16021 ttcgttttct acacccatac cgttttcaga aataaagcag tcgatatttc cataatgatc
    16081 tcgtacattg atcaaaatat catagattcc ttgttcatag atctcccatc cgcgataagg
    16141 attcatttta cgaccaggca tttcataatt gtcgaaataa tcctcaggca ttggaccgtt
    16201 tttagtatcg attggtgttt cttttgcttt tacccggcgt ggctggtaat aattgatacc
    16261 taaaagatct actgtatttt ctttgataac cgtcaaatcc tctgcatgga tcacaggcat
    16321 gagatcaagt tcttttacta tctcaatcaa ttcacttgga aactcgcctt ttacagctgg
    16381 atcaaggaat gaacgattga agaacgcatc tgctatttgc gctgctttaa catcttcagg
    16441 gtttgattcg tctcttggat agcttggtgt cagattcaag atgataccaa taacaccacc
    16501 ttgctccatt tcatggtaag atttgatggc taacgctgaa gccaacgctt catgatagcc
    16561 aacttggatc gcttttttca tatctgactc ttctggataa tgccatcgat ataaataacc
    16621 tgcttcgact ggaacgattg gttcattatg agtaaaccat ttttttactt tcgaaccaaa
    16681 caattcaaaa caacttttgg catatttttc ataggcttca accgtttctc ggttcaacca
    16741 tccgcctttt tcttgcagat acatcggcat atcgaaatga tacagattca caaagggttc
    16801 gataccgttt tcaatcaact catcaaaata acgatgatag aaacgaacgg cttcttcgtt
    16861 gattgttcca gattcatcag gtatcaaacg gctccattgg atagatgttc gaaatgaatt
    16921 atgcccagtc tgtttcatca actgtacatc ctcttgatac ttcgtataaa cttgcgaggt
    16981 tttttcaggt cctacttgat caaagaactt ttgcggttct tttttgtacc aataatcaaa
    17041 aatgttctct ccttttccat cgcctgaaat ccttccttct gtttgtgggc cacttgctgc
    17101 agaaccccac caaaaatttg ctggaaattg atattttttc atcaaagcac ctctttttct
    17161 tttttagttc gttttagttg attccatgta tgatattttc catcacaatc actttttcat
    17221 tttcttcctc ttcttttttg attcgtttta acagctgttc tgctgctcgt tccccgattt
    17281 tatctgttgg tggcgtgatc gtgattttcg gaaggatact cagatcgttt ttatctaaat
    17341 aatcatagtg actgaacgca atatccttcc cgattctgac accttcttga ttgaaacatt
    17401 cgatggcacc aatcgacata ttgtaattgc tgacaaaaat agcagttgcc cctttttcta
    17461 ataatgcttt tgccccgtaa tagccgcttt ttctagaata gtctcctgta aagacctcta
    17521 attggatcga tggatcattt tggaccgctt ctttggctcc tgctaaacgt tctcttgcta
    17581 cgtagtctgt ttgagtggca gcaataaatc cgattttttt atgtccttgt tcaccatgat
    17641 gagcgatcac ttttgctaca ctttctcgat cattcactaa tattgtatca atacctgctg
    17701 gtccttctgg acaattcaat gcgataactg gaatactcat ttttgtgagc atttcaaatc
    17761 cagaccactc ttcttcggat aagaagacaa tcagcccatc gatcacatgc ttcatcaggt
    17821 attcaatttt ttgttcaatt ttttctggct gatcggcaaa cccgcttaat aataagctat
    17881 acccattttc ttcagctact ctttcgatcc ctgagacaac ttccgaccca aaacggctgg
    17941 aaatcccgtt tatcaaaagt ccaatagaga agcttcgatt attcttcaac cctttagcaa
    18001 tgatgttttc tttatagtcc aacgctgaaa tcgcttctgc tatttttttc atgttctttt
    18061 ccttcagttg ctgaccattc aagtaacggg aaactgttcc cactgaaaca ccagccatct
    18121 tagccacatc acggatcgat gccatacatt ctcactcctg ccagttacat ttgatttctc
    18181 tttcttgttg ttcgattatg atgaccgcat tctttgcata gtgttgcttg tcatcaatat
    18241 ccatataact aaagtcttca gtagagcaac ttcctctcac tattaattat acttaaacaa
    18301 gtatttgaac cggttcattt ttaatatagc atttttttaa aagattgcaa cactttttta
    18361 aaacgctttc tgaaagtggc ggattttttg cctttttaaa attttattct aaaatttact
    18421 ctatttgtgg gtgttttgtg cgtttttctc ttccatccgt ttgaagtttt acattccaat
    18481 ggtataattt ctttatccta tcaaatgggg gaagtatttt gaagaaatta atcgtactat
    18541 ttattttgtc tgcgggactt ttgacattga ctggctgtga tttgtttggc ggctctgacc
    18601 aatcctcgga cgaaacaacc gattcttctg tcttaaaagc ggaaaaagca gataccacca
    18661 acagtgtata tacgctaaaa gtacgtaaag atatggattt atccgcggga cagttgacat
    18721 ttacagataa aaaaatggaa tggacacgca cgtatcagac acaaggaact gattcttctg
    18781 aatcatctga agaaaaaaca gtgctgacag atatcaagat cactaccaaa gatgacgaat
    18841 atattatttc aggaaaagaa aataaaaaag aaacaacagt catatttaca aaaatcggta
    18901 attaccgttt gaaggatgag gatggcaaca tctacagtct atagtgccgt ctgttggatc
    18961 ttcttttgtt tctccccttc ttttctgcta tagtaaagaa gcaagatgat tgtaaaggag
    19021 acgaaatgga tgatcgatag tcctgattat tgtacatgta ataacaacaa aaatatccat
    19081 acagaattta atgaccaagg aagcttgagc atatgcgaca actgtcacaa actagtcaaa
    19141 gattcattta aaacatttga atcttatcct gttcaaaatt atgtaaaaag tagaagaaga
    19201 taaacaaatc caagcaaaac tcactatggc tgatttttat gaacttcaaa ctcagcttct
    19261 tcctttgatc aagcttcatc agtaaaacaa acagtggatc atttcagttt acaaatcaaa
    19321 gagtaaagat ttatttattt caaatatttg aatataaaat aaccaaacga tagaaaggct
    19381 tattctacat cacgtagtaa tttttctttc ccgtcgtttg gttttttatt tttagcctaa
    19441 ttcttgacct tttgcatgct ggttatccgt aaaatatttt ccagcaggct ttccttccaa
    19501 ttcagctaat ttttcttgga tctgacggat cacctttttt gcatcttctg gattttctac
    19561 aaaatcgaat ttatccccat ccacttgtat tttaggacat acatcaaaat cttcatacca
    19621 ttgattatag cgggtgttca acgtttcata ataggtatat aacgtctcat cgctttctag
    19681 ctgttcatag tcacgcccac gttttttgat acgttctagc atcgtgtcca aagaaacttt
    19741 gatatggacc atcaaatcag gccgtttttt tggagcaaca tcatctaact cgtttagcat
    19801 cgtatctaat aagctatcgt actgctgtac ttcgatatcc gttacccgac ctaaatcagc
    19861 gtttagatgg aacaacaggc tatcttcata gatggatcgg tctagcacat tgtcatcgtt
    19921 cactaaagca tttttcattg ctaaaaatcg cttgttcaaa aagaagattt gcaataaaaa
    19981 agcgtactgc tcaggatttg aataaaagag cggcaatact tcgttatctt cgacattttc
    20041 ataaaatggt ttagaatcaa tctcttttgc aagcatatcc gtcaaactgc tcttacctgc
    20101 accaatcgtt cctgaaatca aaatcactga catacaaccc ttcctcttct ataagtactc
    20161 gcctacgtaa gtttcaacag gcaatgtttc aaaatcatat tcacgtacag cttggtctga
    20221 ttttaaatac gtatggagac tttgtgtaat catcaagttc gtaggtaccg tctcttcttg
    20281 tacaatgatc atcggttttt tcaacatagt tgccacacct aattcgtaag ccgttcctgg
    20341 atcgattgtt tgatgctcaa agtcaaggat agcaacaaca aattctgcat ttgtcagttc
    20401 ttccatatct ttttcgtata cttcctgcgc ccatccagcg ctgaatagtt cataattgct
    20461 ttcttggtga tgacgtggag agtagaaact tgtgactgtt ttgttttcct ccaacgcttt
    20521 ttcgatacga cttactcgat cgatttgctc ctctgaaaaa aatggtcctg ctaaatatat
    20581 ttgactcatt attttgcctc cttcaataat aactgatttt taatagatac tgctcgtaat
    20641 tcattgacca gttccaatgg attgttggaa aagattactt gatccaacca ttttttatcc
    20701 ataaaatcct cttttgcaaa agtttctaat aattgttgca agccgctgta ataaccgcca
    20761 atattcaata ttccgattgg cttgtcgggc gtcagaccga ttgctatcca agaaagaagt
    20821 tgtgagaatt cctccaatgt acctagacct ccagggaaaa cgaggacagc atctcccttt
    20881 tcaaataata atacctttct ttcatcgatc gtttctgtag gaataaatgt agtaacatct
    20941 tttccaggca attcagtttc aaaaagccca ttcggataga tacctgtgac ttttccgtta
    21001 ttttccagta ctgttttact aatcactccc ataatcccgg agtatgaacc accgtaaacc
    21061 agttcgattt cttcttgtgc catgtatttt cctaacgttt gcgcaataaa tttgtaactt
    21121 tctttattac caaaacggga accgcaaaaa acagtaagtt tcataaaaaa acactcccta
    21181 actcaaaagt ctatcttcat tctacacgtg tttttttaaa ataaaagact agattttgta
    21241 gttttttagt ggtatatagg atagacgcca ctatatatag tatttagaaa aaaatcattt
    21301 ttcagccatt ttatagataa aacaaaaata aataacgtgt tttcatcgat atgaaatgac
    21361 ttacttatag aagatactgg gatttcgtcc tatatgccaa taacgtgtat tattttaaac
    21421 aacaaatact tgtttctttt attaacgaaa aaatcatttt gttctcgtgt tatgtttcac
    21481 tgataaatga tgtttctcta aataaaaaag ccgacgaact aaacgatcaa aatttgatca
    21541 tttttgttca tcagcttctc ctaaaaatct ttttgttaac gataaatagc tggatttgta
    21601 aaaatatcac ttgaatcgtc actgctggaa gaaaattcag tgataaccgc acctgttgcg
    21661 cctgcctgga actagtgttt gatattgggc tcgaacgtgt actgatctct agcatgaaga
    21721 ataatttctt tttttactgt ataataactt tctttccttt ttggaatagt acaggaaagt
    21781 ttttttgttt cctttccttc gacataaggt ataccagacc ctttcgacaa cgaaaggttc
    21841 tcatttttcc ttcatcgtca tttcaaggag ggtgccgatg ctctggacag tttcgatcgc
    21901 tcctaaacca aaatctgtat attcgattct ttctaactct gattctttca taccattcct
    21961 gtctcaaggt acatctattg tacttttttc ttgtaatcca ttctgcctct cctaactaat
    22021 tgtcaaatca tcccaagata tgtcttcttc ctctttttct tcttggatga tttctgcatt
    22081 cggatctact ggttttttca ataataaaat ggttattcct gtgacaaaag aaccagctaa
    22141 caatgcacca atgaatgccc atggtctggt catcgtagcg actgcaaaca ttcccgatgc
    22201 cggaatacgg ctatctgcgc cccaaaacat gcttaatccg ccacctactg tcccaccgac
    22261 tcccgttgct atgacattgc gtgccaagtc gttcaatacg atagggaaac atccctctgt
    22321 gatctgacag atccccatag gaaaagcggt cttcaatgta tcgatttctt gacgattgag
    22381 tatattttta cgaatgattt tccctaagaa ataagcgatc gtcaagccga atggggttac
    22441 catcgaagct aaaatcaata cagtgattgg ttcatagatt ccttctgaaa atcgtgccac
    22501 acccccttat ccagaataaa tggtaatcca gaaacagccg tgtatctgcg gtaataagcc
    22561 taaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatta cttgatgcag
    22621 ggcagctttt tcacaacctc tttttttatt tatgtttcat aatcaatatg gtaaggatgc
    22681 agctccgtat ggtagaccgc ctccgaatat tcaatcattt ccccaatttg cgtagacgat
    22741 ttccgtatcc gtttgatgcc gatctgtgcg tctgttttca gaattccttg ttcttcttct
    22801 gttaaaaata ctgccgagaa atcatcagaa aatgagtcaa tagtatatcc ttttttcgtc
    22861 agcaaacgat acaaagattc tttgccaaat tcttctctgc tgaattgcat catttcttgt
    22921 ggaagataat gaacaaaata aatataaggc tgattgttca aataatataa gcgcttaaaa
    22981 caaacagctt gctcaccgaa aaaggaaaac aaaggatctt ccttctttat atggaccaat
    23041 tgcaaatcca gcgtttcttt ccggacttca tatcgggagt tctccaagat ttgcgtaaag
    23101 cttgccgctt tggataatcg attgtaagga cgattgctta gaaccgtcgt cccacgacca
    23161 cttttctttg cgacatattc atctgatgcc agcagttcga ttgcctttcg gacagtgatt
    23221 ttgctgactt gaaacagttc ttctaattca ctttctgtcg gcaacatcga accgactgga
    23281 tattttcctg agaaaatatc ttctttaatt ttatctgcga cttccaagta tagtacacgt
    23341 gtccttttca taacgtcacc ctttgcttta ctattactaa aagaatagca cataaaaaat
    23401 aaaaaaagaa cacaaatcac atgtgagaag tgtgttcttt ttcattcagc tgctttaatt
    23461 acaagtttac gccatttgag gcaattactt ctttatacca atcaaaagat tttttctttg
    23521 atcgattcag tgtcccattt ccttcatcgt ctagatccac atagataaag ccgtaacgtt
    23581 tactcatttc tcctgttgat gcactgacaa gatcgataca tccccaaggc gtatagccca
    23641 tcaaatctac accgtctgct actgcattag ccatcgcttc gatatgacga cgtaaataat
    23701 cgatccgata atcatcaata atcgtgccat cctcttccat atgatcaatg gctccaagac
    23761 cgttttcaac aatgaataat ggtttctggt aacgattgta taattcattc aatgcgattc
    23821 tcagcccttc tggatcgatc tgccagcccc attcacttgc ctcaagaaac ggatttttta
    23881 ctcctcccag caagttccct tctgaagttg ctgcttgtgg atcagtctca ttgattgccg
    23941 tagacatata ataactaaat ccgatgtagt ccactggata ttcttttagc aactggaagt
    24001 ctttttccgt ttgctctaat ttttccggat cgactccata ttttttatgg gtacgcgctg
    24061 tgtatgcagg gtattctcca cgtacttgga catccgtgca gaaaaaattg aattcttggt
    24121 tttggatcat tgttgctgcc tgattgactg gattggcatc gatgctgtaa gttgttgcat
    24181 agataatcat gcagccgatt tggatatccg aacgcaattc gtggccgatt tttacggctt
    24241 tgctacttgc aacgaattga ttatgccatg cttggaaaat attttggtac tctcctgctc
    24301 cattgctttt tactaatcct tgacttaaag ctggaaagtg aaaggccgaa ttgatttcat
    24361 tgaaagtcat ccaatatttg actttactgc tgtatctctc taatactgtc tgagcaaatc
    24421 gttcatagaa gtcgatcagt tttcgatttt tccagccacc gtattctttt gccaaatgaa
    24481 gaggcatttc gtaatgagaa atcgtgatca caggttcaat atcatatttt aagcactcat
    24541 caatcagctg atcataaaat tctaatcccg cttcgttagg agtaccttcg tctccatttg
    24601 gaaaaattcg tgtccaggca atcgaaaaac gataacattt gaatcccatt tccgcaaaca
    24661 aggcaagatc ttctttgaaa cgatcgtaat gatcgattcc tcgatgattc gggtaaatat
    24721 atttttcttg atcaatcgtc cagtcaaatt cttcactgcc gataatcgca aagcgctgct
    24781 tacctccagg catagcatct gcaacggata gtccttttcc gtctcgatca taagcccctt
    24841 ctaattgatt tggcagcagt tgctccaccc cataaaaatt tttctgaaaa acgtgattgc
    24901 atcctgattt cctccttatt ttacaattgc cagaacatct tcgtttgcaa taatctcttt
    24961 ttggtctaat tcaagtatat cggcaaatgc atcagtattc gtaatgacaa ctggagtgat
    25021 tacgctatag ccagctgctc taattgcttc gatgtcaaaa cgaatcaata aatctcctgc
    25081 tttgacgaga tccccttgtt tgactaagat ctcaaagcca ttcccattta attctactgt
    25141 atccatcccg atatgaatca aaatctccgc tccgtcagtc gttgtgatcc ctacagcatg
    25201 tccagttgga aaaatagtcg tgatctctcc gtctgcaggt gcaactagtt ctcctacttc
    25261 tggatcaata gcgatacctt ttcccatcac gccagaagaa aaaacttggt ctggtacttc
    25321 actcaatggc agtactttac ccgttaaagg actttttaag gtatgccgtg ctgaagtaat
    25381 cggttctcct gcattcgcat gtttgttttc taattgattg tcttgtgttt gttcgtcaaa
    25441 tcctaaaatc aatgtaccta cgaatgcaag aacaaaagca attcctgtag ctattactgc
    25501 tactgtcaca tttgactcta caccatccac cgtattaatg aaagtcggaa tcgtcaataa
    25561 gctgaccaaa ctgctggaga aagcttttac tccggaaaat ccgataatag ctccaccaat
    25621 acctccagaa atacaagcgg caatgaatgg tttcttcaaa ggtaatgtca caccatacac
    25681 agttggttca gtgattccaa ataacgaagt cactgtagaa gaaatcccta atgctcgaag
    25741 tttcgtatct tttgttcgca atgctacagc taaagcagcc ccaccttgtg ccaagatggc
    25801 tggaagaagc attggcatca atacatcaaa cccatattgt tcgatattca aaaacatgat
    25861 cggtacgaat ccccaatgca tgccgaacat cacgaatact tgccataaac ttcccatgat
    25921 tagacctgct acgattggac taaatccata aatcgagttg aagagaccgc ctaaagcatt
    25981 accggcaacc gtcccgattg gtcctaaagc aagaaatgtt aatggaacca tcactactaa
    26041 aacgacaagc ggaactaaaa tcatttgcag aaattgcgga atcacttttt ttacgaatgg
    26101 ctctaattta ctttgaaccc atacggctag aatgattggt atgacagaag aagtataacc
    26161 agatggtgaa agaatcactg ggatcccaaa gaaactgata gcttctcccg caccagcaac
    26221 tgctgtgata gctggataca caagtgccat agcaattgct actgctaaaa actggttggc
    26281 tttgaacttt ttagctgctg taaaagcaag catgacaggt aagaaggtaa agacaccatc
    26341 tgctgcagca aacaatattt gataagtacc gctatcagct gtcagccagc ccatcaccgt
    26401 tgccaatgat aagaatcctt tcaatacccc ggcggctgcc atcgccccta gaaaaggtgt
    26461 aaatatacta gagataatat cgatcagacg attcaaaata tttttggact cttcttttcc
    26521 atcctccgaa actgcaagac cttcctcatc cacgatcgat tgataaacat ctgctacatt
    26581 actgccaatc actacttgat actgaccgcc actttgtacg acttggatca cgtccgggtc
    26641 ttcttttaac ttttgcgtat ctgctaaaga ttcatctttt aatctgaatc gcaaccgcgt
    26701 agcacaatgt actaaacttt tgacattctt ttgtcctcct accgcttccc atacacgacg
    26761 accaactgct tgattatcca ttgttttttc cttctttctt ttttatttaa aaaataaaaa
    26821 cccagattga aaaaaacagt cacaaacatt cgtgaaccta ttttttcaat ccaggttttg
    26881 cctgccatac agtaacaatc cttatgattg gatgcttttt ttctcactta ctactctttg
    26941 aatatgaatc gttaaataaa tctgctctgc gatccccatt tcctgttttt gcgtttgtcg
    27001 aagatactca ttgatccgtt cgacacaatg aaacgctttt ggatactttc cttgaactaa
    27061 tgcgtagaga aattcatctt gctcgtcaga tccttcccct ttgagataac gctgggcaaa
    27121 aaactgcaga tgagtaatga tacgctgata attcaaagat tcttcatcga acacgatgcc
    27181 gaagaattta ctgatgatac tcagtatatc tcttaccatt gtggtagcgg tcatcgtcac
    27241 ttgcatgtct tcgtttcctt gttggctatt cacaaaatga aaggcaataa aaccggcttc
    27301 atcctcagaa agttccacac ccgtcttttg tttcacgaac aaaagagcct ctttcgctgc
    27361 ttgatattct ttcggataaa atttttttgt ttcaaataat aatggattag gcagttggat
    27421 attttctttt gctcgtgtga tcgcataatg aagatgatcg gtcaatgtca gataaatatt
    27481 aggcgaaaat tcaactttta gtttatcttc agccagttcg actacttgtt tgacccattc
    27541 gatctcctct gccggaatat cagccatcag ctgctggaat tggtttttag caaaagaatc
    27601 tcttaaaaca aattcttttt ggaccgcttc tggcgaaatc tcgtccccaa tctttttttg
    27661 aaaagctagt ccttttccca tacaaacgac ttcttccccg aatccatttt tcgaaatgac
    27721 gacattgtta ttcaaaattt tatcaatttt cattttgctt cgccctctca agaaaaataa
    27781 aaaaacctag aattcgccta ctgtttctta gctgaattct ggttttgcct gccgtacagt
    27841 aacaatccat ttcttattca aaatcatcat accatcgaat ttttatttat gcaagccttt
    27901 tcatataaaa aataaaaaaa ctggaacaca agtctatcta gaaaacaacc aaatcagaga
    27961 tgcacctctt tgcgtttctc tttttttcag caaattggtt tttccttatc gattcatatc
    28021 ctttggctgt ttttaatctg taacgactta tgtcccagta tcctaaaatg tttttgtctc
    28081 tagattgaaa gcagatctca tatcatctgt cgcatctttt atcgatgtaa cactttccag
    28141 ttctccttgt acaactagtc gcgtgacggc gtccatgtcg ccacaagtaa ttagtgtgac
    28201 aatttctttt ccctcgactt cattcagtaa ttgggtatct gtaggagcaa ctttttcttt
    28261 aaaaaatgtt ttgtatgtat acacattttc taaatctgtc aaatagatct tggcacccat
    28321 ctctaaattt tctagcggtg taaacagcaa ttcagggttc gttgcacgat ggcttgctaa
    28381 ggcataattt ccttttccca tttcttgatc tggagataat gttcctgctc cgtaaagtag
    28441 tgacgtatta tccaagcctt tgaaaaccgg taaattgatt gaaactgaag ggactgcgat
    28501 actagcaatc actggtagat ctgttcctct catttgcgac ctcatgaccc cttctgtcgt
    28561 cattggttct accgcatcaa agtcaaaact gacatcttta tcgttattct ttttcagatc
    28621 ttcttttgtt acattcgcta tagcatattt atctcctgtc tctctgacaa aataatcctt
    28681 gatctgttca ttgaaaatca aagctagtcc tactaataac aagaggaaca agaggatatt
    28741 gactagccat ctttttgctt ttcctttttt tttcattttc tctccctcca gactcatttt
    28801 actgtttttc ttttgctctt tccaacagcc gaatgacaca tttctacaac tctgtgtatt
    28861 tttttcgtac acctgcgctt ttttctacag gatatttcaa attatttttg atcaattttt
    28921 cttcaatgat ctcatctaga tccaggtgaa ggttgtctgc catcatgtat gaatagatca
    28981 atacatctac cagttcttct ttgattcgtt ctaattgtgt ttcttttact tcttccggcg
    29041 ttttccattg aaaaagttct aacaattcag atgcttccaa agaaatagag atagccaaat
    29101 ctttttcgtt atgaaattgt cgccaattac gttcatctct aaactggttg actttttcca
    29161 tactattcat ttgcttgcct tcttccaatc acttcttact tgattaatca taccattttt
    29221 ttacataaaa aatagatgat gcttttaaaa aaactcctgt tttcttttat ttaaaataaa
    29281 tgtcatagaa aatcaataat ttaggtagcc accatgtgta tgcagtagcg tgtcttcttc
    29341 ccttgaggct ttttgttttt cgatacgttc ttcttcccaa acagacaacg ctactagaat
    29401 ctctcttaat gattttcctt cttcggtgag cgagtactct actttaggcg ggacttggtt
    29461 ataggctttt cgatggatac tttcatcttg ttctaactct cttaactgct gggtcagcat
    29521 tttttttgtg atcgtaggga tttgcttgct tctccacgcc taaaataagc gatattgctc
    29581 ttccctttat ctggtgtaag caaagcagtt agcattttga cgatcgtggg ttccgctttt
    29641 cacttcttat ctacttagta tcatgaagta tactttcgta ctaatactaa gtagcggaag
    29701 tgtcaactac tttgtttaac tacatagcaa aagaaacaga agggtgacaa cggtcatcac
    29761 gcttctgttt cttttgaata tgctgtgttg ttgtctctat ctccgtgtag actttcctca
    29821 aacttatcct ttgacagtaa gtcagtaggc tgatttgctc tttctagatg ccagatacat
    29881 ttctacaaaa aaatagtgat tctgttttga aaattcgatt tttagttctg cattttcgta
    29941 aacatccaac atttttttcg cactgtatag acctaacccg ccattttttt tagaggtttt
    30001 tcctcttttg accatctccg atagcgtcaa attcgttgga tttgccttgt tcgctatact
    30061 aaaacgtgat ccctcttttt cttccagcag attgatcgta atcggactct gatcggctac
    30121 acgatgatcg aatgcgttag aaataaaaat agataaacag cgaataaaaa caaccagatc
    30181 aatcgggatt gttttcactt cgacaggaat aactacttgg atcgggattt tctccgattc
    30241 tgctttttct agaaatttta aaaagacacc cctgacagct ggggatacaa tatttgttag
    30301 ctgttgtatt tgcggttttt caaaaatagc tgcagaatga tcttctaata tttctaaaaa
    30361 ttccaacgtt tcttttttat tttcctgaag agtaattttt aatgaaaaca gtagactttt
    30421 ataatcatgt ttgaattcct ctagatgatg gtaatattct tcatttattt gatacagttg
    30481 aagcgattcc tgatgttgtc tttcttttac ttggaaactg atcaacataa tggaaaaaaa
    30541 caaagaaaaa aaactgatga ctatcgacaa aagagataga agcagataat tagatgagcg
    30601 gaagtgaaca cttcccaata ccaagtaaat cacgccccag cttaataaaa tacaaatacc
    30661 tccaaaatag tgttcagttg gataagtaaa aagcaagttg agaaatctcg tctgtttgtc
    30721 tatacttttt actaatagat agaaaataaa tgcgataagt agtgcaaaaa aacttgctgt
    30781 tccaagatgt ctctttccta aataatccat cagatcaaaa accagaaaaa agctgagtcc
    30841 agtgatagca aaaaaaagat tactgatcaa acagctgata aacaggtttt tcgtagtaaa
    30901 ataaaaaaga aacatcaata ataagccaac taggtaagga aagctcactt tagggatagg
    30961 aagtaaaagt gtcaccacca tcaaagctat acaaccaaag ctgtttttta ctagcagttg
    31021 tttcgaaaag aataaataga tacctaaatc taaaatcaaa gtaaaggtca aataaagtgt
    31081 cactgttgcg accatcttat tcctccattg ttttgtttca aaatgtacac atattttttt
    31141 aataggagcc tgcatatgaa aaagtatttt atcatagaag ataaccagtt tcacagcgaa
    31201 tggatcatca gagaattggt caatcatcat atcgagaata tcgtgcagct cacttctatc
    31261 tctcaaatca atcagttgat ccagttgggc actattgata atcacgatgt tttctttatc
    31321 gatatcgact tgaagcaatc actcacaggt attgatgtcg ccgaacaaat acgtaaaaaa
    31381 aatgcacata gtaatattgt ttttttcacc gcttatacga attttgcagt agagatcatc
    31441 aatcggaaaa tccttccgat tggatatatc agaaaagaca ggaatttatc tgaacaaatt
    31501 cgtcaagtgc tagaacagat caattggaaa gaacagcaga tcgtaaatgt cgataaaatc
    31561 tttattgatc aaaaaggtgg aagtctcgtc ctgtatcctc gggaaatttt atatattgaa
    31621 agtatcaaag gtttgaaaaa ccaagttatg gtcaaaacaa ctacagaaga taaattagtg
    31681 aatacttctt taaaacaatt gaagacagag ttagaaaagt atgcttactt tttactttta
    31741 aaatcttact gtatcaactt atcgcaattg cagaaaattg attctgctca ttatgtgtta
    31801 gaatttttca acggtgactc cttactcgtt ggtcgaaaga tctttgaaaa gacaaaggaa
    31861 cgtttccatg acttccaaaa aactgcttcg agttgatgaa taagagctga gacgtaagca
    31921 tagcagattt cagggagtgg gccttgaatc ggctccactc cctgaaatcg ttgatatgat
    31981 tttacgcttc ctcttctgta ggagcatctg ttaacgacca tgtgaatgtt gaaacgtagt
    32041 tgtctacagc ataattggat acaggaacaa ataattccac tgattgtctc ccttgttcca
    32101 cattctctcc taatgaataa atccatgtgc caatcccttc atttgctcct gcagttacta
    32161 gatctactgc ctgatctgct gggatttcga ttccttcagc gccagttcca gaaacggtag
    32221 atggcattcc actgctatta gttgcgctat ttcgaatcac ggcgtttttg aatttcacct
    32281 ttgaaccagt tagtttattc gtatcagatg ttgtaccaat aaattctgtt cgtttgactg
    32341 ataatttcca accggcataa tttccgcttt tatccgttac ttgtacgaaa ttctgtactt
    32401 gtgtttgtgc tccagtactg tccgttccgc tttgtaatgc agcatatact gtttttgctg
    32461 tacctaactt gattgttcca aaatcaagat ctggagcttg gtcaatcgtc agtaatccgg
    32521 atgttccctg atttggttcg tcaggtgtaa aaggagtatc aggatcattt ggatcaacag
    32581 gaggaactgg aacaacgtct tcaggatttg cagcatctgg tttggtcaca gaaatcgttc
    32641 cttctgtagt ggctgagctc ttattctcag caaaaacact ttgtgtacat aataatagac
    32701 ttccagctag aacggtactt aatgcaattg tttttttcat ttctttttcc ttctttcttt
    32761 taaaataaat aatccagcaa ataagccaat aagaatgact ccaacaagta tcagccatcg
    32821 ccagtcaaat gtttctggct gattttttaa atcaatatcc ttttcattca attggtccgc
    32881 tgcatcatca gttatttcaa aatcggtatg gagattccag cgtttttctt tccaagcacc
    32941 cttcacaacc gcttgataat gtcccggagc taattttttt ccttctaatg ggacaagtaa
    33001 atcaaaattt gtattgggtg ctacatctat tttttctttt tttattgaaa ggtaaggctc
    33061 ttttgtttct tttttgtaga tttcaaccga tgtagtcaag ccgaacatat aagaagcatt
    33121 tggattttga agattcgcct taatcacatt tcttccatcg ctttgatccg cataagcttt
    33181 ttttaatttc aaatctgggg aagactcttt ttcgctattt caaatcaaga ttgctttcac
    33241 ttgagaaaac tgattgacga caccaactgt acttttgtta gaaacgttct cttttttttc
    33301 ttgaaacgtg atccctcctg cgaccacacc agccagctct ttttctggcg ctttcagtga
    33361 aagtgtctca gtgaccgcac tttgaggagg aacagttatt tctcccactg tttttgctaa
    33421 gtttttcata tcataactta atgtttcatc aaatttactt tttgattctc catattcgat
    33481 cacaccgttt gaattcgtcg tagcggagtg gatcaatgtc tcaactgtta ctgatttatc
    33541 tgttcggtta aagaccgtgt aggggatttc ctgtgttttc ccaggctcca tccttacatt
    33601 tatatatgtt ttgttcttat caatctgatt agccggtaat tcaaaatcta ccgcaaaatt
    33661 gaattcggag gcatggacag gtgtctgcca aaggatgaat ccaaggagaa ggatcataag
    33721 taaagagcag ttgtttcgat gacttttatt ccatattcga ttcatcaatc atcctccttg
    33781 atctttattc tgtcggcgca tctgctaatg tccatgttat ctctgctgag taattaccag
    33841 ctatctgatt ttctttggtt aagacaagtt taaacgcccg accgttattt aaatattctg
    33901 aataatctgc ttgcacattc gttgaatttt gtcctctgac aatttccagt tcttcttcat
    33961 tcactaattg atactcctct cctttcttga aatataggac atcctgcata gaaaatcctg
    34021 catcatttgt gaaaggctga gataatttta tcgttaagat ccacggattt ttcgtttctc
    34081 gctgatcata aatccataat ttttcctgta tatccatttg gccaaatgct tcttgtgtca
    34141 aaccagttgc tttgatctga ccaaaatcga gtttggaagg ggcaagaaat gagagaccac
    34201 cttctactac gatcttcgta gcaggtgcaa aaggcggggc atcatgataa acaactggcg
    34261 tatcacttgg attgtgattc ccaatttcat cgttggtact tggcttgttc tgcacaccct
    34321 tagcagtagc ttctcctgct tggtcgttta atacaatttc taatacatct cctgcaaaaa
    34381 gttgattttc tgggatagaa agaacaaatt cccctgtatc atcaatagtt gtttctgaca
    34441 aaacagcttg attttttttc aatgaaacca aggctcctgc ttcacctgta cctgtgatcg
    34501 tgcgagaagc tgcattgatc aatgtggtac ttaaattagc aggttcagga ggaatcacat
    34561 cgactgtcgt tttggcagag tccagttctg tcacttcttg gccacctctt acttttttag
    34621 cagcaacaac ttgcacttga tcctccgttt ttaatctctc atcattgggt acttgaactt
    34681 cgaacaagcc gccttgttcg ccttctcccc atgggttata tcctgcccct tcattttcat
    34741 ctgtttgtcc ttgcgtgaga gcagtaattt ctacagatga tttcgtcgta tctgcgtaag
    34801 taatacgtaa agtcacttct acttcgcctg tccaagcatc tcttggcttt gcctcgacac
    34861 cttcgggaac aacgacatgc ccatagattt ttttatctgc attcgttggc actcttaagg
    34921 attcaatcat cgggagctga ttattggcac tgattcgatt ataatttttc attccggcac
    34981 ctgtctgagc aaattcattt gttaaacctg cactagggct tgatacgaaa ttgcctaagt
    35041 cggtcccatt gatcgataag tctgtcatat cagaaaaata atccgggtct ttttcaaagt
    35101 tatcgaatgt tgcgcctgtt tttcgccata aagcgatttc tgaatgggcg atctttaaag
    35161 aagaatctcc atttccttgg aatacccagc cgcctatgcc attggtctcg cttccaatcc
    35221 gtttgttttg gaaatcaaaa taggtaggag attctaagtc cacattcaac acatccccac
    35281 tgatgatgcc ggtcttatct tcccctgttt tcccgaccat gactaaataa gttttttcac
    35341 ctgccgtaaa atttaaatca atatctccag ttgtatctac agttgctccg gaatctgcgc
    35401 gtatatcgat atttgatttt tctccagtga ctgtaaaatc agctgaacct ggactttcta
    35461 gaaaaccaga agcgtcataa aattggatcg cttgatttcc ttgattgagt ctgttcacgt
    35521 aagatttgcc atcacctgta tttcttaccc tcaatgagcc tccattggtt actgtcagct
    35581 tttgcgtacc attctcaaaa cgaactgcgc tggaacgccc actctgctta tcgatgtcga
    35641 taactgatcc ggctccttct acgatcaaag aaaggccttt tcctacaaaa cgcatcccat
    35701 tgttcccggg ttgatcgtca tctccagcca ttttgaccga gatttgtcca ccacttgata
    35761 catggatttg actagaatct ccttgcaagc gaaatccgcc cgtagttgct gtattgatgg
    35821 tgattttccc accattcgat acagtgactt tcgatcctga acctctcatt tggatgattc
    35881 cgcgattgtt gtcattggta atattgatct ttgtatttac cgaaaattcg cttcccgaac
    35941 cactgacttc aaacatgaca ttaccaggta gcgtatcgac tgcctctcct tcatcgctaa
    36001 cgccatctgc ccgatcataa tcaagagaga cctttgaacc gcttaagata tggattcctg
    36061 gcgctgtttt tccactacca gtctcatctc ctgaatctag aaaaccgtag aatttcccta
    36121 gatacggtcc ataaagttga ctaccattga tcacattcaa ttgatagctt ctacaaaaat
    36181 aggctttacc cggagcggca ttcagtcctt ccttcactgg ttggacatcc aacacaccgg
    36241 aaacaccatc taatgtgaca gttgcacgag gagcgtaaat cagtcctaat ttggacccat
    36301 catacaaatc aagtgtcccg gtaaatttta tttctcccgt aaaatgatat ctgcttcccc
    36361 aaaaatcggt catataagca atgataatgg catttccatc gatatttggt gtcaatccac
    36421 ctggatgtgc agctgttcct ttaaaattct ggaactcgat cgactgaggt atctcattga
    36481 tataaatatg gtgtgtgccg aaattgtgag cgaatccacc aaaatcaata acgatatctc
    36541 gtttgatctc taattccctg tctaaagtca aaggctgaat cagtcggatc ttagttactg
    36601 attcgtctgc gagaagctgc gccagttctt ctgttgtggc agcttcgctg tacccatccg
    36661 ctgaaacagc tcttgccagt gccgcttcac tgctatcaga cgtttgttca gctacttctg
    36721 aggtctcact tgtcttattt tcttcttgcc ttttattagt agaagcctca ctttgagatt
    36781 ctgtagtttc agaagtaatc gattcttctg aaaaagtagt cttgtcaaaa ctttgattct
    36841 ctgattgcgt tgattcgttt gttttctcta catcggcaaa aacggatgtc accatcaaga
    36901 gagggaagac aaacaaaatc gacgcaatca gcttttttac acattgattc attctccacc
    36961 atcctctctg acgttttgac aattttattt tatcgtttta atctatttcc aattgtactt
    37021 tcatatttaa aatatgtatt tttatggaaa aatttttcta ttaggtgtaa atttcttttt
    37081 tgtacaaaaa aaaggctgtg acaaaagtct tgtcacagtc ccttatcccg aacaagcggt
    37141 gttctcccgc caactcctcg gtcacaacct ccattttaac aataaacctg taaatgtgag
    37201 ggcaatactt tgattgtcac aggtaattta tccccttcat ctccatcaac attcgtcgtc
    37261 aattcttcct catcagctaa tgacacaact aattgctcac aagtaaggta ggctagattt
    37321 ttcgtagatt catctactcc ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa
    37381 gaagaatctt ttaaataaac caaatggagc ttaccatcat ccacttttgc atttggaagt
    37441 aatgtttcaa aaccgccgac tgagttagtt agtcctatca gcaaggtact gctctcaatt
    37501 tcttccttct ttccatcaac ttctacgtga aacgagtagg attgtgtgga agctaactgt
    37561 ttgattcctg acatgaaata agctagtttt ccaaatttcg tcttcttctc cgcatctaca
    37621 tcattgattg attcagggat agagccaatt gcgacgacat tcataaaata gtcgtcattt
    37681 atttttccaa tatccaatgg cttgactgat tcaatcgaaa aatgattgat cgcttcctca
    37741 ggttctagcg gtatccctaa tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat
    37801 ccgaaatttg gccgatgttc ttgttcagcg attccactga ttccttcgtt gactgttcca
    37861 tctccgccca tgacaaacac actatgatac ccttccgtag ccgcttcgcg agtaaagttt
    37921 tttgcatccc ctgctttttt tgtatgcaag acgaccactt catcaaacac cgattccaat
    37981 tttgctatcg ctaattgctc aaattccttt gcttgttcac cgcctgaact ggggtttacc
    38041 actaataaaa cttttttcat cactttcttt cccttctgat tgatttcttt aatataacga
    38101 tagcataaag aaaaaaatta ggctgtgatg aagtactgtt catgaaacat tcatttattt
    38161 tttgacaaga taaaacatcc atactatgaa actgaggaaa attaaaacaa tgatatagga
    38221 aataaaagaa aatgataggc attgtatacc taataaagta aaaatcttag gtggcagata
    38281 aaaacacaaa aatagcaaga gaatgtaata gcgtttcttc cactttttgt tatcagacct
    38341 tatagcgatc agaggaatac caaatgcata actgaaaaac tcttgatgta ctgttaatat
    38401 gtcacggcct tgaataataa atgcgacaat ccccgctata attagtgtgc ctaacaccaa
    38461 aacggaaaat tgatgaatat attttatata caaatgatcc attgtacttc ctgccttttt
    38521 atttgtgaga aaaacaaatc cgtatacgtg aataacttct agaaataaaa gaaatttgca
    38581 aaaccactcc atttatctac ttctacgtgc attttttgat attgttttct ttatttaaga
    38641 ttatacagaa aaatatctag ggtcgctgga ttctaagtaa aaaaaatttt taaaataaaa
    38701 agaactaaat caagtagtaa ttaaactact gttgatttag ttctaataaa aagatttaga
    38761 tgaacagttt tattgattta aatagttcat attttttatt tataccgctt atttactact
    38821 tgctgtacta gaatctgaag tgaatgtact caaaacattt tcaaagtctt tgtctttgat
    38881 ttttacatta gcgtctttca atacttcgcc aattacttta ttctggaagg tactgtcact
    38941 taattttgtg tctgttgcaa tttctttcaa ttcttttttg tatttatcca tgtcgttgcc
    39001 tttgttttgg tttttcacca ttttaaccac ataatattcc gtagtataag ttgaagcatt
    39061 ggtagaagtg atcacatcag aaacttgacc atctttcaac ttaaaggctg cttctttgac
    39121 ttctgccgga accgttgtag tagtggaatc aaatttcact tttccgccat cttcttttgt
    39181 cgtagtatca gttgacttgt ctttggctaa tttagagaaa tcgtctccct tatcagcagc
    39241 tttcttcaca tcttttgcat catcttcact tgctacttgg ataatttgag cttccacttc
    39301 tggatggaaa gattcccaag ccgtttttaa atcttcatcg gtcaatttga tatgtttctt
    39361 tagaccttct tgaaaggcta gactttgttt gatactgtct ttgaatgttt gttctgtgta
    39421 acctgcagat ttcaattgtg aatcaaatga atcacccaat tgtttctttg tttgatcata
    39481 ttgagaatcg atttctttgg ttgatacgtc atctccatat ttatcttcaa atacttgata
    39541 aacgatcaac tgttgcacag cggaatctgc tccttgcatt tttttcaagc tatccattac
    39601 ttcttgttcg ctgattgtac cgcctttata actaacggct gttttttcac cggcattcgt
    39661 acagcctgac aataagccag tgcctagtac gatggcaagt gctaaaaatc tttttttcat
    39721 ttatcaattc tcctactttt cgttttttgc gaattgctaa aaacaaaggt gttcaagatg
    39781 aaaatcatca agaacacctt tactaatctg ctcgatacgt cctgaataga tataaggcta
    39841 cacaatccag aaagttcgct cagcagaata aatgtcaagt agcacatcac accttaatat
    39901 actacttctt ttgttaccgt ttttcaagtc tatcttgcgt tccgtattta acgtaactaa
    39961 ttcttagaac aaatgttatc gattttgttt ttcttcttcc gctgctggga tattcaaatc
    40021 agacactttt atgttcgatt ttccagacat ttttttgttg tttttattat
//
LOCUS       DACEZN010000009        36933 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEZN010000009 DACEZN010000000
VERSION     DACEZN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.009"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..862
                     /locus_tag="IXH60_000335"
     CDS             <1..862
                     /locus_tag="IXH60_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1989304.1"
                     /translation="AFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLAL
                     GENVTEFITDEEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVN
                     EYMQTSNPDIFAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQ
                     GTSGLYLFGWTIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKG
                     TNRIVGAQFMSKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQ
                     AALGEIETAQ"
     gene            1033..1320
                     /locus_tag="IXH60_000336"
     CDS             1033..1320
                     /locus_tag="IXH60_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989305.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            1546..1989
                     /locus_tag="IXH60_000337"
     CDS             1546..1989
                     /locus_tag="IXH60_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1989306.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(1995..3179)
                     /locus_tag="IXH60_000338"
     CDS             complement(1995..3179)
                     /locus_tag="IXH60_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1989307.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            3447..4145
                     /locus_tag="IXH60_000339"
     CDS             3447..4145
                     /locus_tag="IXH60_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1989308.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            4152..4829
                     /gene="nth"
                     /locus_tag="IXH60_000340"
     CDS             4152..4829
                     /gene="nth"
                     /locus_tag="IXH60_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1989309.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(4955..5488)
                     /locus_tag="IXH60_000341"
     CDS             complement(4955..5488)
                     /locus_tag="IXH60_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989310.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(5785..8166)
                     /locus_tag="IXH60_000342"
     CDS             complement(5785..8166)
                     /locus_tag="IXH60_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1989311.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(8156..8803)
                     /gene="recU"
                     /locus_tag="IXH60_000343"
     CDS             complement(8156..8803)
                     /gene="recU"
                     /locus_tag="IXH60_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1989312.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            8867..9406
                     /locus_tag="IXH60_000344"
     CDS             8867..9406
                     /locus_tag="IXH60_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1989313.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            9496..9915
                     /gene="gpsB"
                     /locus_tag="IXH60_000345"
     CDS             9496..9915
                     /gene="gpsB"
                     /locus_tag="IXH60_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1989314.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            9937..10304
                     /gene="rnpB"
                     /locus_tag="IXH60_000346"
     ncRNA           9937..10304
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IXH60_000346"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            10513..11679
                     /locus_tag="IXH60_000347"
     CDS             10513..11679
                     /locus_tag="IXH60_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1989315.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            11725..13221
                     /locus_tag="IXH60_000348"
     CDS             11725..13221
                     /locus_tag="IXH60_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1989316.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            13302..13985
                     /locus_tag="IXH60_000349"
     CDS             13302..13985
                     /locus_tag="IXH60_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1989317.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            14630..15820
                     /locus_tag="IXH60_000350"
     CDS             14630..15820
                     /locus_tag="IXH60_000350"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1989318.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            15922..16875
                     /locus_tag="IXH60_000351"
     CDS             15922..16875
                     /locus_tag="IXH60_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1989319.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            16944..18293
                     /locus_tag="IXH60_000352"
     CDS             16944..18293
                     /locus_tag="IXH60_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1989320.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(18476..18799)
                     /locus_tag="IXH60_000353"
     CDS             complement(18476..18799)
                     /locus_tag="IXH60_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989321.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            18928..20091
                     /locus_tag="IXH60_000354"
     CDS             18928..20091
                     /locus_tag="IXH60_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989322.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            20161..21510
                     /locus_tag="IXH60_000355"
     CDS             20161..21510
                     /locus_tag="IXH60_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1989323.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(21555..21986)
                     /locus_tag="IXH60_000356"
     CDS             complement(21555..21986)
                     /locus_tag="IXH60_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1989324.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            22374..25016
                     /gene="alaS"
                     /locus_tag="IXH60_000357"
     CDS             22374..25016
                     /gene="alaS"
                     /locus_tag="IXH60_000357"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1989325.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            25216..25812
                     /locus_tag="IXH60_000358"
     CDS             25216..25812
                     /locus_tag="IXH60_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1989326.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            25934..27082
                     /locus_tag="IXH60_000359"
     CDS             25934..27082
                     /locus_tag="IXH60_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1989327.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(27120..27533)
                     /locus_tag="IXH60_000360"
     CDS             complement(27120..27533)
                     /locus_tag="IXH60_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1989328.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            27612..28043
                     /locus_tag="IXH60_000361"
     CDS             27612..28043
                     /locus_tag="IXH60_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1989329.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(28142..28375)
                     /locus_tag="IXH60_000362"
     CDS             complement(28142..28375)
                     /locus_tag="IXH60_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1989330.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            28558..30228
                     /locus_tag="IXH60_000363"
     CDS             28558..30228
                     /locus_tag="IXH60_000363"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1989331.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            30375..30761
                     /locus_tag="IXH60_000364"
     CDS             30375..30761
                     /locus_tag="IXH60_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989332.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            30748..31446
                     /locus_tag="IXH60_000365"
     CDS             30748..31446
                     /locus_tag="IXH60_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1989333.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            31451..31915
                     /locus_tag="IXH60_000366"
     CDS             31451..31915
                     /locus_tag="IXH60_000366"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1989334.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            31912..32823
                     /locus_tag="IXH60_000367"
     CDS             31912..32823
                     /locus_tag="IXH60_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1989335.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            33058..33597
                     /gene="pyrR"
                     /locus_tag="IXH60_000368"
     CDS             33058..33597
                     /gene="pyrR"
                     /locus_tag="IXH60_000368"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1989336.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            33611..34894
                     /locus_tag="IXH60_000369"
     CDS             33611..34894
                     /locus_tag="IXH60_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1989337.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            34907..35833
                     /locus_tag="IXH60_000370"
     CDS             34907..35833
                     /locus_tag="IXH60_000370"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1989338.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            35852..>36933
                     /locus_tag="IXH60_000371"
     CDS             35852..>36933
                     /locus_tag="IXH60_000371"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1989339.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAE"
BASE COUNT        12157 a         6448 c         7817 g        10511 t
ORIGIN      
        1 agcttttgtc gaatctggaa aacaagtgac attgattgat ggattagatc gcatcttgaa
       61 taaatatcta gataaaccat tcacagatat cttagaaaaa gaattgacag accgcggtgt
      121 tactcttgcg ctaggcgaaa atgttactga atttatcaca gatgaagaag gaaaagtgaa
      181 acaagtagct acaccaacag atacatttga tacagatatg gtcattttat gtgtgggctt
      241 ccgtccaaat acaaaactga tcgaaggaaa agtagataca ctgccaaacg gggcaatcaa
      301 agtgaatgaa tacatgcaga caagtaatcc agacattttt gcagctggag actctgcagt
      361 agtcaactac aatccaagtg gcacgcaaaa ctatattcct ttggcaacaa atgccgttcg
      421 tcaaggtttg cttgtgggaa ataacttgac agatcataaa atggcgtatc gtggtactca
      481 aggaacttca ggactttacc tatttggctg gacgatcggt tcgacaggtg tgaccaaaga
      541 aagcgcaact ttgaacgatc tagaagtcca agcgactgta tttgaagata attatcgtcc
      601 agagttcatg ccaactactg aaaaagtaat gatggagcta gtctatgaaa aaggaacgaa
      661 tcgtatcgta ggtgctcagt tcatgtccaa atacgacatt acacaatcag caaataccat
      721 gtctttagct gttcaaaata aaatgactgt agaagatctc gcactttcag atttcttctt
      781 ccaaccacat tttgaccgcc catggaatta cttgaaccta cttgcacaag cagcgctagg
      841 cgaaatcgaa actgcacaat aaagaaaaag ggttgtgaca caattttgtc acgaccctct
      901 gtcttttttt gtgaaaagta caattagttg tctttcttcg aattttttgt cacaataagc
      961 agcaaagata aagctgttac ataaaagaac aagcagcttt atttgatttt gaaaagaaag
     1021 gtgagaaaga agatgaataa agaatatttt gtccaacaag atgcattttt agaagattat
     1081 aaaagtgctt cgaagaaatt aacgatcaat attgatgagt tggcagatca agtactctct
     1141 cagttgaatg aaacgcaaga ggaatattac acgatgccag caaatgacgc caaagataat
     1201 gtttctcatc gttttccttt cagtagacgc atctactcag atgaaaatga cggcacattg
     1261 aatacagagt acacatatga aggtcagcca tatgtgctag aaactgatga tgatctttaa
     1321 agacagtttt tgaaaaggcc gctcatcctt aagtgatgac aaaaaatcat cacaattacc
     1381 gatgactgtc tctttactat cttttaatca gaaaaagggg ttatgacaag ctttgtcaac
     1441 cccttttttt cttggtttca gattgggttt tcctacaaaa tatttttatc tttgttgaat
     1501 aggaaacaaa aaaacaatat aatgaaagaa aaagaaggtg tagaaatgga taaaagacaa
     1561 ctgctaatag aagaatatct ggatttagct aatgagatta ttcatcgtaa taaaccagaa
     1621 atggaaaaag aatttgcagg atttactcta aatgaactag aagtgattga acatatcggg
     1681 aaaatccctg atcctaacgt gacaaaatta gcgaatgcta gttacatgac tagaggagcc
     1741 atcagtaagc taacaaaaaa actgatagct agagggatca taaacactta tcaaagcgaa
     1801 gagaataaaa aagaaatcta ttttcaattg acgccaatcg gacaaaaaat aaggaataga
     1861 cacgagcaac tccatcaaaa atgggtacag cgagatgcta ctgtatttga aaatatgtca
     1921 aaagaagaat ttgacacagt atttcgtttt atcggtcgat acagagaatt tatacgaagt
     1981 agcaaatgaa gctactattt tttattgtag tgataatagg caaaaatcat aagcaaaaat
     2041 ccgaataccg tcggaagaaa tggtgctgca gcataaagct gtcctgcgat tagcgggccg
     2101 atgacgcgcg atagagattg tatactttgg gtaccgccca ttacaagacc ttgctggtta
     2161 tctgagacag aattggatag ttggccattg aatacaggag taaaaataga atcaccgaaa
     2221 ccataacaga taatcccaaa taaaaacaaa atcggcagta gaagaattcc agataatgtg
     2281 atgaatagat aggcgaggat ttcactgaac atccctattc ttgtgatctg ctgttctgaa
     2341 aattttttga gcaatcgagg cataatgaac aattgcgaga cagtatccaa gattccaatc
     2401 aaagagaagc ttaaaccaac caacccagcc ttccactgaa aggcgtcaat cgaaaattga
     2461 gaaaagatag attgaagaga gccagctgcc aaccaaacag caaaacctgc tgtcaataaa
     2521 ggaataacgg atgaaatctt gaataattgt tgtaattggt gaaaaggtct tacatgggag
     2581 aaacttaaat ctactgaacg tttttttatt ggaaggcttt ctagcataaa tgtgtagcca
     2641 taaacggcat ttaagaaagt aaagagtgct ccaataaata taggaacaga attccccaac
     2701 tcagctaata gaccccctat aataggaccc agcgttgttc ctataccgac taaagcaccc
     2761 atccatccaa aaactttggt tctttcatta ggttccgtga tgtcggcaaa gtaagcatat
     2821 aaggtggaga tttctcctgc cgtcaatcct tcaatgatac gaccaaggaa caacatccat
     2881 atcgaatttc ctaaaccaaa aagcaaatat ccgatacttg atcctaataa actgataata
     2941 aggatcggat gacgcccaaa atgatcactt aagctaccga gaatgggggc agataagaag
     3001 gcagctagag cataaacaga catgagcaaa gtgatataga aagcttgctg atgtatggag
     3061 tgagaataag gcagcgttaa aaacggaagt acaggactga cgatagtaaa tcctaatcca
     3121 gtgaataaga cagagacgta tccaaaaact ttagaatgtt tatttgtgaa actgtacatg
     3181 taaaaactcc tttatgcaat aatgttaact tggaaacaaa aatagtgtat agtaattttg
     3241 tttccttgtc aacattctaa ggaacagcga aatctactta atttatttat tccgaaaagt
     3301 gaagatacgc atcaaaaaca tgaacaaaca aaaatcaagt agacgacatt gtaaatctga
     3361 ccgatcagtg aagtaaatag aaaagaaacc tgttcttgct tcccatgttt tttttacgta
     3421 tactgtgatt gagcggagga gaaatcatgt tgaatttaga aaattactta agcgctggac
     3481 aaacagtcat atctaatctt ttacttaaaa attatcatag gatcggtcta caatctgaag
     3541 aatttttatt tgttctacag ttgcatatgg cacagcttga aggagatact ttccctgatt
     3601 tgcagatgat tgctatggat atgggaatca aacaagatca gatttttcaa atactggatc
     3661 gtctcgtaac aaatggattt atcaagatag aaacgacgat tgtcaacggt aaaaaagcag
     3721 atcgatataa tctttacccc atctatgatc tattaggtga gtatttaaaa acacaagaaa
     3781 aaaaatacga agaaaaaaca caagaaaaag aaatccagtc agtgtatcaa ttatttgaac
     3841 aagaatttgg acgcccgctt tcttcgatcg agttccagcg gatcggacaa tggctagaag
     3901 aagaccatta tcagccggaa attttgaagc tagcactgcg ggaagccgta ttaaatcagg
     3961 cttatagttt taattacgtt gaccgcattt tgctatcgtg ggaaagaaaa aatctacgta
     4021 caaaacaaca agtggaagaa gaccaaaaac gaagaaaaca gcagcttctt caaagagaag
     4081 tagaacagtc agctcaacaa gaagaattgc cgaaagtctc actgaaaaat tggttggagg
     4141 agtagcggaa tatgttaagc aaacaaaaaa caatggaagc tctagaaacg atgtatggaa
     4201 tgtttcccga agcacatgga gaactcaagc acaataatcc atttgagtta ttgattgcag
     4261 tgatcttaag tgcccaggcg acagatgtat cagtgaataa agcaacgcca gatttgtttg
     4321 cctcttttcc gacacccgat gcgttagcag aagcttcaat cgatgaaatc attcttaaaa
     4381 taaaaacgat tggtttgtat cgaaataaag caaaaaacat caaagcgtgt gcacagcagc
     4441 tgattgaacg tttcgatggg caagtgccaa cgtcaagaga agaattgatg tctcttcctg
     4501 gtgtaggcag aaagacagca aatgtcgtat tgggggatgc attcgggata ccagcaatcg
     4561 cagtagatac ccacgtggaa cgtgtctcaa aacgcctgag gatctgcaaa ctagatgcta
     4621 cggtcatgga agtagaagaa acactgatgc ggaaagtacc acaagaatta tgggtaaaga
     4681 cacatcatac actgattttt tttggtagat atcattgcac ggcaagaaat cccaagtgcg
     4741 aggtatgtcc tttgctatca atatgtcaag atgggaaaaa tcggatgcgt ctaaaagaaa
     4801 aggcgctaaa aaagaaacca cgcctgtaga cgtggtttct ttaattgcaa ataaatgtat
     4861 gaattatctt gttattatgg agaaaagctg tttcgtttat cttttttgca tgaaaaagta
     4921 aatgtacata ctcagtagct aggatggtaa cttattacaa tttattgcga taaaaataaa
     4981 agttgttatt tttattggga aaatacccga tgtagaaaat aatgccttcc aatataccta
     5041 taaaataagt ccagtattcc aaaggattat ataaataagt gacgtatcta tctaaaggtg
     5101 taataaaaat gataagtgat aataaaaaaa taccatttat aactaaacat aaactaatgg
     5161 atttgggcgg tttcaggctc atcagaaaaa tatagattct ggtgaacaac aaccataaaa
     5221 aagtgagaat cattgcatga gagcttcttt ccataaattt tcctttaaga gtagtgctta
     5281 aaagtacaga aaaaataata aatgcaagga taggagcaat taaagtaaat ttatttaata
     5341 ataaacaaaa tttttcagct tttttattct tggaataacg aggtattgtt ttcgataaaa
     5401 aaagatggtg cagacaaatt atgataatta agcatactat cagataactc aaaatgaagc
     5461 cttctttcaa aaaattattc aaaaccatat attgtatttt ttatttgata ataacaatgg
     5521 gattattaag aagtggcact aaataaaaga ctagtgtgta tttagccgat ttaattataa
     5581 acaagtaaat ggataaagta tatgtaatga taaaagaaaa atggattttc taatttttaa
     5641 gctaaatgtt agatcacaca tgtatatgct ggatagtctt ataaaaaatc atgtaataca
     5701 gcttggtaca tgatttttat ttgtagctgt tttttacata gacacaaaaa agaaaccacg
     5761 cttgtagacg tggtttcttt tttactatct gtttccagaa gctgaggtgc tgctggatgg
     5821 tgtatggtca tttgcagcat ccgctccgct agttgctggc tcactggaag aagcaggagg
     5881 agcagatgaa gacggtgtcg agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga
     5941 cgctggctcg cttgtttctg ctgtcgtgct ttcttttgag gactcagcag ttgattcgct
     6001 ttgactagta gagctagagc tttgagtcgt tgtctctgtt gttgagcttc caggagtctg
     6061 gacatagctg gaagatggaa tagttgttga tggtgtaatt gcattcgatc tagctgtata
     6121 ttgatctttg tagtacagtt caccgcccac acgaattaat ccgcttggca tctcccagtc
     6181 tgtatttgtc acgttagctg aaacatactg cataagctcg cgataaacat cagaggctac
     6241 gtgtgacgat tctgaagtga caggtgtcat ttttttattg taacctgtcc acacggaaat
     6301 cgaataattt ggcgtatagc ccgcaaatag gatatctgga tacaccccag aagaaatacc
     6361 aagttttgca tactcgtcat ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa
     6421 cccagcgatt tgcgcattcg taccagttcc ttcagtgatc gtatctttta aaatgtctgt
     6481 gatcatataa gctgtttctg gagacatggc tgtttttcca tctggctcat aagtttcttc
     6541 tgttccgtct tggaagacga ttttattaac atattgaggt ttgtaataag tccctccatt
     6601 ggcgaaagcc gcataggctg ctgccatttt taaggaagaa gctccgtatt ttgtcccatc
     6661 ttgttcttcc gtattactag agatcgcatt cgattgatgg atcgtactgt attcgatccc
     6721 aagatttttt aagaaagaag ccactttatc tgaaccgact tcattgaata atttgactgc
     6781 tggaacatta cgggataagt aaagtgcttg gcgtaaagta atggtgccca tatattgatt
     6841 atcccagttt cctacaggag tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt
     6901 ttttccagta gaatatttca aatattcaaa agctggtccg taatctgtga caggtttcat
     6961 cgtagaacca aaatcacggg aagtattcac tgctaagttg ttgcctaaag ttacgtcttc
     7021 tgcgatgtga cgtccgccaa tctgggcttt tactttcccg gtattggtgt cgataagggt
     7081 ggaggcaact tgcatctcgt cgtctgggta agagacatac tgatccgtgt tgacgatatc
     7141 atatagtttc ttttgggcgt ccagatccaa attcgtataa atctctaaac cgtctgtgta
     7201 gacattctta tctgtttttt cttgtacttc attgatgact tctttgacat aattatcgac
     7261 gatcttagta ttgtcatcgg attgagttag ttcttgcaga ccatccgtta ccggtacatt
     7321 aacagcttga tcatattctg tttgagagat tttttcgttt tgaagcatcg tatacaaaac
     7381 agtatctcgt cttttcttcg cctgatctgg ataaacataa ggatcatagg cggaaggggc
     7441 ttgaggcatc ccagcaagca atgctgtttg cggtaaagaa agttctgata atttcttacc
     7501 aaagtacatc tcagaagctg tctccatccc gtacaagccg tttgacatat acactttatt
     7561 tacgtagtag gtcaagatct cttgtttcga ttttttttgt tccaatctta cagccatcca
     7621 cgcttcttgg gccttgcgtt tcagagtctg atcctcggca ctggtggaga agaaggaaag
     7681 tttgatcaat tgctgtgtca aagtacttcc gccttgcagt ccgccagagg taaagttaga
     7741 gagggcagaa ccgatgatcc gaatagggtc aaccccgatg tgtttataaa aacggcgatc
     7801 ttctaccgac acgatcgcat cttctaatgt tttcggtaat tcattcgcag aaattttttc
     7861 acgcttttct gcgcctaaat cttcgaatag ttcgccatct tgtgtatata gtttggaaga
     7921 tacggtagcg tctaattttt tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa
     7981 aagtcctaca cctgctaaaa agaggataca gaaaaatgag agtattccta aaaggatctt
     8041 gataaataat ccttttttat gtgttccaga ggactttcca gaacctttgc cagaattctt
     8101 cttcacactc tttttaggag taggctgttt ctgtcgtcta gagcttctag tttgttcatt
     8161 tgccattctt gctttctcct tcaatagata atgttgataa atagaagtca accgcgtcca
     8221 aataaggaat ccgcggagaa ataccagttt tgatctctat tcctagttct tcgatcaaag
     8281 ataaaggtag tgatttgcgt cctttttcct tttgggcgtc ccagtaagca atcaaatgaa
     8341 cactattcaa ataaaaacag cgcttcaaag acgaaaacca taacaagaca aagcagaccc
     8401 cattttgttc gaggcatgct ttcatatgtt cgatttgatg ggcatggaag tttttaaagg
     8461 gaaaagatgt tttgttcttg gtttcttttg cttcaaagtc caagtaaaag cctcgataga
     8521 cgccattata gtctgtagtt gaggcttggg taaagtaggc ttccttgatc acagcagcac
     8581 ttctcttagg atagtctact ttcacgattt ggataggggt aggcttttta tggataaccg
     8641 ccaacccgcg ggaaagataa tatttattac tttcattgat tgcttcttca aagcgcatcc
     8701 cccgattgcc gaattcagtg atctgatttc ttttttcttg aattttttcg acgggagagg
     8761 cattcttata ataaggttgg ccatttggat agcgcaatac catctttaat cacactccta
     8821 ggccacatta tagcaaaaaa gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa
     8881 agtaatctat gtgtctgggt atcgaagttt cgaactcggt gtttttaaag aaaatgatcc
     8941 taaaatccaa gttgttaaaa atgttttaaa aaaagaactc catcagttag cagaagaagg
     9001 gctggaatgg gtacttgtca gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt
     9061 agcggaattg aaagaagaat atccggaact atctcttggt attttgtatc ctttttctga
     9121 ttttggtaat aactggaatg aagggaacca agaaaaaaaa caattagcag aacaattagc
     9181 agattatgtc gaagctgtta gccatcaacc ctaccaatca cctagtcagc taaagaatca
     9241 cactcgtttt atacttgagc atacagtggg ctgtctgctg atctatgatg aagaattccc
     9301 tggaaaaacg aagtattttt ttgaagatgc acagaattac caagcagatc atccctacga
     9361 aattcggctg atcagtatgg acgatcttca aaattttagt gaataatcga tattgtgttt
     9421 gatttatcaa cttttttcct atacaatgaa aaaggtagaa atgttattac gtaaatgaaa
     9481 actagaggtg taaatatggc gaatttggtt tatagtccta aagacatttt acaacaagaa
     9541 ttcaaaacaa aaatgcgcgg ctatgatccc gttgaagttg atgaattctt agacaatatc
     9601 atcaaagatt atgaaactta cagcaaagaa ctccttgctt tacaagaaga aaacgatcga
     9661 ttgagtgcaa aagtagctca attatctaaa actcaaggag cagctcaaac acgcgtgcaa
     9721 caaacagagg caccaaagag cgcagcagtg accaattttg atatcttaaa acgcttgtca
     9781 aatcttgaac gtgaagtatt tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca
     9841 gcacaaccta atccaaataa ctacacaaat gcagacacaa gcttagatga taatgaaaaa
     9901 acacgtcaat tttaattttt gattaatgaa tttgcggttt tcgggtaatc gcggtctgac
     9961 ttgatcaggc tgaggaaagt ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct
    10021 tagcgaaacc ataagctaag gtactctttg gagtaacggc aggaaaacaa gctaaggctt
    10081 cggctatgct taagtatcct tgaaagtgcc acagtgacga agcggctggg gaaacccatc
    10141 cggtggaacg cggtaaaccc ctcgagcgag caacccaaac aatggtaggg gcgctttttg
    10201 tctggaagaa gaacaggaca aaaaggcagt ataactgcag atagatggtt accgctttgt
    10261 tttttctccc tggaaaagca aagtacagaa catggcttat agaaaaccgc aaacatcagg
    10321 gaattaggtc gtgacaaaag taatgcgtta taagaaacaa gaaaaacatt tgaaagctat
    10381 ttttcaatac ttcagatgaa tcgttcttat tacaaaacaa acttttgtat caccggttat
    10441 tcggaatttg agtaaaagtg tggggtagga ctgatgtccc acctcctttt tttataaata
    10501 aggagattgt gtatggaaaa aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg
    10561 ctagaagcac tggttggtaa agaattaaga gatcttggaa tcgaatgcca agttgagaac
    10621 ggacgtgcac gttttgaagg aacaatggaa acgatcgcta cagctaattt atggttacgt
    10681 acagcagatc gagtaaaaat cgtggttggg gaattcgatg cgtatacgtt tgatgaattg
    10741 tttgaaaacg taaaagcatt gccttgggaa gattttcttc cactagatgc agcttttcct
    10801 gtagctggaa aatcgatcaa gtcgaaacta tatagcacac ctgattgcca agcgatcaca
    10861 aaaaaagcaa tcgttgaacg tctccgtacc tattatcacc gtccagcttc tgtaccactg
    10921 acggaaactg gcgcacattt tcagttggaa gtagctttgc tgaaagacca tgtgatggta
    10981 actctggata cgacaggacc aagtttattc aaacgtggtt atcgcttaga aaaaggtgga
    11041 gcaccattga aagaaaacat ggcagcagca cttattatgt tgacaaattg gcgaaaagac
    11101 cgtccatttt atgatcctgt atgtggttca ggtacgcttt gtattgaagc agccttaata
    11161 ggccataata tcgcaccagg attcaatcgt gagtttgctt gcgaatcttg ggattggttc
    11221 agtcaagaaa tcatggaaaa tgttcgatca gcagccgaag aaaaagcaga ttatgatatt
    11281 gaattagata ttactggttc agatatcaat ggacggatga ttgaaattgc caaagccaat
    11341 gcagaagaaa tcggattagg ctcttcgatt acctttaagc aacaagcagt caaagacttc
    11401 aaaacggata aagaatacgg ggtcatcgtg gctaatccgc cttacggaga acgtctagga
    11461 gaagaagaaa ctgttcggaa attatataaa gaaatgggcg atgtattccg tccattaaaa
    11521 acatggagta aatatatctt gactagcgac ttagcgtttg aggaatttta cggacaaaaa
    11581 gcaaccaaaa aaagaaaatt gtacaatggt gcattgcgta cggatctatt ccagtattgg
    11641 ggaactcgtc cgcctagaaa aaaagagaaa gacgaataat cttactaatt gattcattca
    11701 attggatgtt tggaggaata gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa
    11761 atcaatcttc tacaacaagc tttaggaatc ctcgattggg atacacaaac aggtatgcct
    11821 gaaaaagcta gtgcataccg aagtgaagta gacagctatc tttacagcat ttatttttca
    11881 aaaaaaatcg gtccaaaaat caaagaagcc attcgctatt tttctgaaca tccggaagaa
    11941 ttgtcggaag ttggaactgc cgtttttgcc aaagtaaaag aagagtatga tttggaacat
    12001 cgcgtaccgg aagatcttat gagaaaatat acagctgccg tttcttcagc acacggacaa
    12061 tggcaaaaag ctagagaaac acaacaattt tctgattttc aacacgcatt gaatcaaaat
    12121 attgaattaa caaaacagtt gataccttac tggagaaaag atgaaaagac gaattatgat
    12181 gttttgctga atcaatacga acccgggatg actgtcgaaa tccttgataa ggtatttgat
    12241 cagcttaaag aaggaattct ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct
    12301 gaaacagact ttttaaatcg gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa
    12361 gtaatcgaac aacttggtta tgattttagt cgtggtcgtt tagatgacac tgtccaccca
    12421 tttatgactg gaattaatcc gaatgatgca cgaatcacga cacgatggaa cgaaacagat
    12481 ttcaaaatgg ctgtttttgg agtgatccac gaggctggtc atggtatgta tgaacaagac
    12541 atcgatgata aatatgcgta tacaccggtt tatgaaggta catcaatggg tatccatgaa
    12601 tcacaatcgt tgtttaacga gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa
    12661 tatcctttct ttcaagaatg tgcagaagga acattcgatg atattccttt tgacaaattt
    12721 tatcgttcat tgaaccatac aaaatcaagt ttggttcgta tcgaggcaga cagtttgact
    12781 taccctctcc atatcatcat tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta
    12841 gatgttgctg aactacctga aatatggaat caaaaatatg aagagtatct gggaatacga
    12901 ccaacaaatg atgtggaagg catcctacaa gacgttcatt ggtcaggtgc aagctttggt
    12961 tattttccct cttatgcatt agggtacatg tatgcggcac aattacttca ttctttgcag
    13021 caagaaatag atgtggaaaa tgttctagcg tcagacgatt attctccaat caaaaactgg
    13081 atgtcagagc atatccatca atatggtgca tctagaaaac caaatcaatt aatcatggat
    13141 gcaacacatg aaccgcttaa tccgcaatac ctgatcgact atatgaaaga cctgtatttt
    13201 aatgtatatc aggtaaaata aaatacaatt tacaaagata gctgaaattc atctaatcta
    13261 tagatgtttt cagctattta cttaagaaag aagtggaacg aatggataaa ttacaagtga
    13321 tcgctgatta tagtatggac caattagcgc atgatcaaac aggacatggg agcgatcata
    13381 ccaaaagagt tgtcaagtta gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct
    13441 ttgtgacgct tgcagcagct tacctacatg atacgattga tgataaagtc gttaaaaatg
    13501 agaacgaggc aaagcaacag cttcgtgtat tccttcgctc tttgccaata gtagaggaac
    13561 agatctcgat gattttttcc attatcgaaa atatgtcctt ttctaaaaat ctctctgaag
    13621 cggccgaact atctctggaa ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag
    13681 gagcaatcgg cattttgcgt actgcttatt tcggcggtgg acatgggcac ccgatttttg
    13741 atcctgaact gtatccgcaa acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag
    13801 tgatcaatca tttttatgaa aaactgttct tgctgtctga taaaatgaat actgattacg
    13861 gatatgaaga agccaaacga agagaaacct tcatgcgtga attcttagaa gaattttttc
    13921 tggaatggtc tattgacgat gaaaatttta ctccagacaa ttggatcaaa aataacaaaa
    13981 aatgatggag gttgtgacag aagtggtcag ctccaagata tttcggatca ttttccgtaa
    14041 aagttacttc tggaacactg tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc
    14101 acaaggaaca atttcaaaaa atagcttctc gtgtttgatg aatattgtaa cttgtgattc
    14161 ggaattggca ggagaacacc gtttattcgg aagaatagga ctgtgacaag acttttgtca
    14221 cagtcctttt ttcgaattca acaagtaaaa cttttaagaa atattcagag agtaaaatta
    14281 aaaattgctg ataaacgaag atggaaattt tatgtatttt tattggtcta tgcaataaat
    14341 aaattaaata ttcagaatac aattgatttt aacgaaaggg cttctatcaa tggttgtgtt
    14401 gtatttatct gcccaggtag agtaaaatag atcattatct aaaaggtaat tgttcatact
    14461 atataagtca tttgaaagta aaaagatact gtaaataaaa aatgtggtta aataattctc
    14521 ataatcagtg taagcgttat caaaaatata gcatacttgc tttgtagatg aatgacacga
    14581 tatatgtttg gtatgttctg caagacagat gaatatggag gtggagacaa tgtcttccaa
    14641 agaactaaca atgtatttag agaaagaatt ggatcaactg aaagaaaagg ggttatacaa
    14701 tacgatcgac atactagaaa gtgaaaatgg tgcttgtatc atcgtagacg gaaaaaagat
    14761 gatcaatcta gcatccaaca attatcttgg atttgccaat cgcgaagaat tgaaaaaagc
    14821 atgtattgaa gcaaccgaaa cttatggtgt aggagcagga gcagtccgaa cgatcaatgg
    14881 ttcgctgaaa atccatcagc aattagaaga aaaaatcgca gaattcaaag ggacagaagc
    14941 agcaatcgcc tttcaatctg gattcaactg caatatgggc gctatctctg cagtaatgac
    15001 aaaagaagat gctatcttat cggatgaatt aaaccatgca tctattattg acggttgccg
    15061 cttatccgga gcaaaaatca tccgaattaa acatcaagat atgaaagacc ttgagaaaaa
    15121 agcaaaggaa gcgatagaaa gtaagaaata taaaaaaatc atgtatatca cagatggtgt
    15181 tttctccatg gatggagaca ttgcacgatt gccggaaatt attccgattg ttgaaaaata
    15241 tggactgatc acctacgtcg atgatgcgca cggctcaggc gtaactggaa aaggtgcagg
    15301 aaccgtcaag cattttggat taagcgacaa aatcgatatg caaatgggca cgttatcgaa
    15361 agctataggt gttgttgggg gttatgtagc tggatcgaaa acactaatcg actggttgaa
    15421 agctagaagt cggccattcc tattttctac ttcgctaact ccaggtgcag ctgcagctgc
    15481 attggcttcg attactttga tgcaagaaca tccggaactt gtcgaaaaag tttgggaaaa
    15541 tgctaattat tttaaagaag aattgaaaaa agtaggctat aacatcggta tgtctgaaac
    15601 acctattact ccagtcattt tgggagacga aaaagtgact cagactttct ctaagaagct
    15661 gatcgaacat gggatttatg ctaaaccaat cgtttatcca actgtgcctc ttgggacagg
    15721 caggatcaga aatatgccga ctgcggaaca tacgagggaa atgcttgatg aagcagtagc
    15781 agtttatcaa aaaataggaa aagaaatgga aattatttaa ctaaatttag ttttttcgtc
    15841 tatttaaaat tagagaacat gttcacgcat atattgttaa tcatctgaag atagtagtaa
    15901 aaaagatcag ggaggatcat catgaaaaaa gtattggtga caggatgttt aggacaaatt
    15961 ggttcagagt tgatcacacg gctacgtcaa gaactaggca gtgaaaatgt cattgcgaca
    16021 gatatacgca tgcctgaagt cattgagaaa aacgatatct ttgagatcct agatgtaatg
    16081 gattacgaaa aaatgcgcag catagcagaa caatatcaag tggacacatt gattcatttg
    16141 gctgctcttt tgtcagctgt tgcagaagca aaaccacaat tagcctggaa tttgaacgtg
    16201 actgggctgg tcaatgcatt ggaagtcgct agagaattcc agctgaaatt ttttacgcca
    16261 agttcaatcg gcgcgtttgg tccaaatact ccaaaagatc ataccccaca agatactctt
    16321 cagcgtccta caaccatgta tggggtaacg aaggtagctg gagaactact atgtgattat
    16381 tattatgtga aatatggcgt agatactcgt ggtgtccgtt tccctggatt gatttcttat
    16441 gcaaccttgc caggaggcgg tacgacagac tacgcagtcg atatttatta ttcagcaatc
    16501 aaagaaggaa agtacactag tttcattgat caaggtacat caatggatat gatgtatatg
    16561 ccagatgcga tcgatgccat tataaaactc atgaatgcag atcctgatcg attggttcat
    16621 cgaaatgcct tcaatatatc ggcgatgtct tttgacccgg aagagatcaa agcatctatt
    16681 caaaaaatca taccagattt tgaaatggat tatgatgtgg atcctgtacg tcagtcaata
    16741 gcagaatctt ggccaaattc actagatgtt tcttgtgcgc aagaggaatg gggcttttcg
    16801 ccgaaatacg acttagatgt gatgacaaag gatatgttgg aaaaactgaa agaaaaattg
    16861 gtagagacgg tataaaacaa gaaccacatc caaaaagttt tttatacttt tgggattggc
    16921 tatggtatga aaggggacaa gggatggaag aatatcaagc cacgccagta aaagaagtaa
    16981 aaatagaaaa tgaattaaaa cgtacgatgg gattttttac tgctttatcg actgttatgg
    17041 gcacagtgat cggtgcaggg gtatttttta aagccgcaag cgtagcagaa gttacaggat
    17101 ccgccagttt gcatatgttt tcttggtttc tcggtggtat gatttctgtc tgtgcaggac
    17161 taacaggagc tgaactggca gctgctattc cagaaacagg agggatgatt aaatatatcg
    17221 aacggattta cggaaataca gcagcttttt tattgggctg ggcgcaagtc gtgatttatt
    17281 ttccagcaaa tgtagcagcc ttgtcgatca ttttcggtac acagttcgtc aatctatttg
    17341 gtctttccca gtcaatgatt gttcccgtag ctgttacagc tgctgtatcg atcctgttga
    17401 tcaatttttt aggttctaaa gctggagggg cttttcagtc gattacgctt gtctgcaagc
    17461 ttattccttt gtttgtgatc gtcattttcg gtttgttccg acaagaagga gtagacttcc
    17521 aactgtttcc gatccaagca ggagaaaatc tttcattttt ctcagccttg ggagcgggct
    17581 tgctagcaac gatgttcgca tacgacggtt ggatacacgt aggaaatatt tcgggagaat
    17641 tgaaaaagcc ggcgaaagat ttgccaaaag caatttcttt aggaatcatc ggaatcatga
    17701 tcgtttattt attagtaaat gcggtgttct taagaaccgc atctatcgac ggagtcgcag
    17761 gaaacagcaa tgcggcaagt gacgttgcaa aaatgatttt tggcggcttc ggtggaagac
    17821 tggtcacagt tggtatcttg atttctgttt atggaaccat caatggctat acccttactg
    17881 gaatgcgtct cccttatgtt atggcaaaag aaaacaacct gccttttagc aagctctttg
    17941 ctaaacttca tgataaaact aaagttcctg tagccgcagg tatcttagag ctagtcatcg
    18001 ctattggcat gatgatgatc ggtggttttg atacactaac ggatatgctg atctttgtca
    18061 tttggatttt ttatacgatg gtctttgttg gtgtcattct tcttcgtaaa aaagaacctg
    18121 atttgtttcg accatacaaa gttccaatgt atccgtttat tccactagta gcaatcattg
    18181 gagggacttt tatcgtatca agcacgctta tcactcagac ctttttagct tcgatgggga
    18241 tagtattgac acttgctggt atcccgattt atctctattt aaaacgtaga tagctaaaac
    18301 attcgaattt tattagaact gcacttctct gccaaacaaa atattttatt taaatgagga
    18361 gtacgtggaa gaaatgtcaa aagtactcgt taattttgat cacgtaaatg tcgttttgtc
    18421 tacgatgaaa tagcaaagta aaagacagaa atcgggctca accgatttct gtcttttact
    18481 ttgatttgcg taactgaact attcggcgta cgccgcgata aatatttaat aaaaccaagc
    18541 aaccagaaat gaaaatggga agccaaatca gatagacagg gtagccaagc actttagaca
    18601 aagcatgtgg agtcatttta tccatttcaa gaagtgcttg ctgccaagtc tgaagattga
    18661 agactagcat gaaacccgaa ataatcagga gaataaatcc taaataatga attgtagttc
    18721 tttttcttac ataagcgatc gtagcataaa ggaacagtaa aaaaataggt acgccaaaaa
    18781 caaagtacat attcagcatc tcgacacccc ctttgtgttt atcttacttt actttgaaag
    18841 aaatgagaaa taatagcctc gttacttgct tgaaacagca aaggactgct aagataagag
    18901 agatacaaag aatcaaggag gagtcatatg gcaagttata tcgaaccgat tcgttttgcg
    18961 ctgattgttt ttccattttt agccttagcg atctcatctg ttttttttat ttatgaatat
    19021 cgaaagtatg gaacatttct agtaacaagg gcagttattt tgtattcttt cgttttttat
    19081 ttattgtgtg cgtatttttt ggtgatctta ccacttccgg caaaggagac ggttgcccaa
    19141 atgacagggc caaagatgga gctgcattta tttgcttcat ggcaacattt cagagacaac
    19201 acggtattgg tcttgtctga acctagtacc tacctgccgg caatgaagca gagtgtattt
    19261 ttggaaccag cttttaatgt tttattactc ataccatttg gcatttattt aagatattat
    19321 ttccggcttt cttttatgaa aacgatgatt gctagtttct gcctttctct attttttgaa
    19381 ttgacgcagc taagtggact ttattttatc tacccaagac cataccgctt atttgatgtg
    19441 aatgatttag tgacaaatac attgggtgga atcattggtt ttgtcgttac gccgttattt
    19501 acatttatgc ttcctacaag aagacgaatg gatgaacttt cttatgaaaa aggaaagact
    19561 gtctcattga ctcgacgctt ggtcgcttat ttgatcgact ggacattttt atctcttatt
    19621 aatttggtag cggcgatttt ctttaaaatt gcgttcgatt caggggcagt tacttctcat
    19681 atctggtggc ttttattaga agtaggggta tactttatga ttgtaccgtt tataaccaat
    19741 ggatggacac ttggcaaaaa aattgtgcgt atccgtttgg tggaagaaga ccgcgaacga
    19801 atttctttaa aagcattgat gatccgctac gggtatctgt actttctttt ttatggttta
    19861 agctggttgg ggcagttgct tcagcaaatc atgaattcga caacgaatac ggtgttgcag
    19921 atgagtgcag tattcatttc tctcggtgtc ttgttactac aaggtatttt tattttaaat
    19981 atcttgctat catttttgag aaaaaaacgg attctgtttt atgaaaaagc tagtcacaca
    20041 tatactgtta gcacgattgg ccaaacaaag gatgatgatt ctcgaaatta gctgagcaaa
    20101 aacgctttgt ctgtgttata atgccaaagt cgtgataatt taaagagaag aggtgcacga
    20161 atgaataatt ttgatatatt ccgcttcaaa ccgtttatca atgaagcgtt aaccgcaaag
    20221 ggcttcaaac agcccacaga ggtccaggaa cgtttgattc ctttaattgc aaaaggaaaa
    20281 aatatcattg ggcaatctca aactggtaca ggaaaaacac atacgttttt gttgcctttg
    20341 tttgaaaaaa tcaatgcaga aaaagaggaa gtacaagttg ttattacagc tccaagtagg
    20401 gaactagctg cacagatcta tgaagcagct acacaaatcg ctaaattttc tacaccggag
    20461 atccgtgtct cgaattttgt cggcggtaca gacaaacagc gtcaattaaa tcgtctgcat
    20521 caccaacagc cacaggttgt agtaggcacg cctggtagaa tattagacat gatgaatgaa
    20581 caagcgctga agattcacac cgcatttgca tttgttgtcg atgaagcgga tatgacattg
    20641 gatatgggtt ttttaaatga agtagaccaa attgcaagtc gattaccaga aaagctgcag
    20701 tttttagttt tttctgccac tattccagaa aaacttcgtc cattcttacg aaaatacatg
    20761 gaaaatccaa tcattgaaga aatcaaacca aaagcagtta tttctgaaga cattgaaaat
    20821 tggctgattt caacaaaagg taaagatgag aatcaaatga tttatcaact gctgacaatt
    20881 ggtcatcctt atttagcaat cgtatttgcc aatacaaaac agcatgtgga tgaaattgcc
    20941 gattacttga aaaaccaagg cttgaaagta gcaaagattc atggagacat caccccaaga
    21001 gagagaaaac gagtgatgcg acaagtacag aatctggagt atcaatatgt tgtagcgact
    21061 gatttagctg ctagagggat cgatatcgaa ggtgtctctc atgttattaa tgcagaaatt
    21121 ccagaagatc tagacttctt tatccatcgt gtcggacgta ctggacgtaa tggcttaaaa
    21181 ggaacggcca ttacgttata ttcgccaaat gatgagcaag ccattgatgc gatagaaaaa
    21241 ttgggcgtct catttgaacc aaaagaaatc aaaaacgggg aaatcgtcaa aacatacgat
    21301 cgtaatagac gtgccaaacg agaaaaatca aaagatgtat tagatcccac gctgattggt
    21361 cttgtgaaga agaaaaagaa aaagatcaag cctggttata agaaaaaaat cgattgggca
    21421 attgcagaaa aaaataaaaa agaacgcaaa atcgaacgca gacagcaaag ccgtactgct
    21481 cgtaaaaata agaaaaattc acataaataa aaaacaagag gttgtgacaa aaggcgtgtc
    21541 acaatctctt gtttttaagc tagacttttt ttcattccga tacaaagtac ttcatcttca
    21601 ataaaaggtt ctgtgcaagt atgataccct tgtttttcat aaaaatctac agccgtcatt
    21661 tcagctacta cataactagt ctgacaacct tctgaaaccg cccgtttttc agcagcttca
    21721 agcaactgac cgcctaaccc ttttcctcgg tattcttttg ctacgcaaaa tcgttccggc
    21781 ttgaagcaag taggtgtttt cttttcataa cgcaaagtag ctactggtct tttttgatca
    21841 agaaatagat aataggtttg ttgcgggata tcttttccgt cgaattctag ttctgcagga
    21901 atcccttgtt ctaatacaaa aacttgatat ctcaaaaagc agctagctgc gcgtatccat
    21961 tcatccgctc caactatttt tttcatcata ttcctccttg tcatgaatag cagagatgta
    22021 ttgattcctt tattcttatc gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa
    22081 taaaaattga gatattgaca gtgatcaata gaatgcgtat actaaataag tacgaaaagg
    22141 atatgatttg ttttttaaga ctcacagaaa attctttctt ggctgaaaaa agaatagtca
    22201 ctaatcaaat tgctcccttt ttctattttg gtagaaatac cgacgcagct cagcgttatc
    22261 gagtttaaga ggtaatgatt acacatcatt gcaatcaagg tggtaccgcg agttttcgtc
    22321 cttggctgtg atggtgtttt tttgtattta atttatagaa agcaggtaat gacatgaaac
    22381 aattaagtag tgcacaagtt cgacaaatgt ttcttgattt cttccaatct aaaggacata
    22441 cgattgaacc tagtgcgtct ttgatccccg tgaacgatcc aaccttattg tggatcaact
    22501 ctggtgttgc aacattgaaa aaatattttg atggttcagt tgtgccagaa aatccaagaa
    22561 tcacaaatgc ccaaaaatct attcgaacaa atgatatcga aaatgtcggt aaaacagcac
    22621 ggcaccatac gatgtttgag atgctgggta atttttcgat tggtgactat ttcaaaaatg
    22681 aagccatcca ctgggcatgg gaatttttga ctgatccaaa atggatggca ttcgatccag
    22741 aaaaacttta tgtaacagtt tatccaaaag atacagaagc aaaacgcatc tggcatgaag
    22801 aagttggcct gccactagat cacattattg atgtagaaga caacttctgg gatatcggtg
    22861 caggcccatg tggtccggat acggaaattt tctatgaccg tggagaagct tataacgatt
    22921 tagcagaaga cgatcctgaa aactatccag gaggagaaaa tgaacgttat ttagaaatct
    22981 ggaacttagt attttcagaa ttcaaccata agcctgataa cacatttgaa ccacttcctc
    23041 ataaaaacat tgatacgggg atgggattgg aacgcatggt ctcaatcgtt caagatgcgt
    23101 caacgaactt tgagacagac ttgtttatgc ctattatcca cgccgtagaa aagctaagca
    23161 aacaagtaaa atatggtgaa aacccagtaa cagatatttc ttacaaagta atcgcagatc
    23221 atattcgcgc cctttcattt gctattggcg acggtgcatt gccatcgaat gaagggagag
    23281 ggtatgtctt acgccgtttg cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg
    23341 aacaagcatt cttatatcgt ttagtccctg ttgttgggga aatcatggta agttattatc
    23401 ctgaagtact tcaacaaaaa gaatttattg aaaaagttat ccgtacagaa gaagaacgtt
    23461 tcaatgaaac aatcaatgaa ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa
    23521 acgaaaacgg gacgactcta gcaggaaaag acatcttcaa actatacgac acatatggct
    23581 tcccggtaga attaacggaa gaggtggcag aagatgaagg actgaaagtt gatcatgctg
    23641 gttttgaaaa agaaatggat gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa
    23701 aatcaatggg ggtacaatct gctttattga cagatatcaa agtagaaagt aaattcattg
    23761 gatacacaga acaagaatca gatagtcaat tattggtcat cgttaaaggt gagtctcttt
    23821 tgaatgaagt cgcagaaggt gaagctcaaa tgatctttga tcaaacacct ttctatgcag
    23881 aaatgggtgg gcaagtagcg gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag
    23941 cagttgttac agatgttcaa aaagcgccta atggtcaatt tatgcacacc gtagaagtaa
    24001 aagcaccaat gagtgaagga caaacctatc atctattgat cgatcaatcg atgcgcaatc
    24061 ggatcatcaa aaaccataca gcaacccatc tactgcacaa ggcattgaaa gaagtacttg
    24121 gcgagcatgc aaatcaagct ggttctttag tcgctcctgg acatttacga ttcgacttta
    24181 cccactttgg tcaaatcact tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa
    24241 aaatctggga agctatccca gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca
    24301 tgggtgcaat ggctttgttt ggtgaaaaat acggacatga cgttcgagta gtaaatattg
    24361 gcggctggtc aatcgaactg tgcggaggca cacatgtagc aaatacagaa aacatcggaa
    24421 tcttcaaaat cgtttctgaa tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa
    24481 caagtaaaga agcgtacgaa ctacttgaag aagaagaaaa acaactaaaa caagtagcaa
    24541 caattgttaa atcacctcaa ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc
    24601 aagtaagaga gctacaaaag gaaaatgaac aattagctag caagttagcg aatcaacaag
    24661 ctggtgatgt attcaaagat ccagaaacga tcaatggaaa aacaatcatt gcagcacaag
    24721 taaaagtgaa agatatgaat cagctgcgtc aattagctga ccaatggaaa caaaaaaata
    24781 tttcagatat tcttgtttta gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa
    24841 tgacgaaaga atcaaatgaa gctggactaa aagctggcga cttgattaaa gcaatcgcac
    24901 cgaaagtagg cggtggcggt ggtggacgtc cagacatggc acaagctggc gggaaaaatc
    24961 ctgctggtat tccagatgca cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg
    25021 tttacgtttg acagaaaaac aaaagttcaa acattgaacg aaacatgatt atgggggcga
    25081 agacaaacaa gtcttgccct catacttttt tcaattggat ggcgcatcta atcaaatggt
    25141 cggattcgga tggtctttag ttgacgaaag tgacagaaat ttttacgatt agtataaata
    25201 aaaaggggtg aaaaaatgaa tagtagaaat aagacctttc gactagtctt acgtgcgatc
    25261 ttgttagcaa tcatcatcgt acaagcgatg gttccttggc tggggtttat tccattagga
    25321 ttcattagtt taacaattat ccatatcaca gtaattattg ctgcagtagt attaggccca
    25381 aaagatggca tggtgattgg cttgttttgg ggaatcgcta caattgttcg tgcctatgcc
    25441 atgccaacaa cgccatttga tacattagtg ttcacgaatc cgattatctc ggtagttccc
    25501 agagtacttg ttggcttagt agcaggcttg gttttccact ggatttatca aaaaaataaa
    25561 tcaataacga tcagctcagc ctttgcaggg gtactgggtt cattggtcaa tacagtctta
    25621 gtgttaggat tcatgggatt attttataca ggtgcaacgg cagatgccta tggtgtagac
    25681 tctgctcttt tatttaaaac cttagcaggg gttgcagcaa tcaatgggat accagaagca
    25741 ataggtgcgg gaatcataac accattacta gcaaaagcgt tatttgcagc aacaccgttg
    25801 aaaccagaat aatgaccatc ttttaatagt aataggacgc acgatagata gaaaaaggaa
    25861 gagaggagag gaaaacaatc gtcctctcca tctttttacc tgagcaacaa gcaaactaga
    25921 aggggtaaaa aacttggcta agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg
    25981 gacaaaaaaa ggagtactag cgggtatctt aggattggtt acgaatctga tcttgtttgt
    26041 ctctaagcta atgatcgggc ttttttcagg aagtgtctcc attatggctg atgcagtgaa
    26101 cagtctatca gatactgctt catccatatt gactttgatc ggatttaaaa ttgctgcaaa
    26161 gccagcagat caggaacatc cttatggaca cgaacgcttc gaatatatca gtgggttatt
    26221 tgtttcgatc atcattactt atgtaggatt tcaattttta gagtcttctg ctgaaaaaat
    26281 catccatcca gaaaatgtct ctttaacacc catcgtcttt tttgtcctaa tcatttccat
    26341 ctttttgaaa tttgcacaag gcaaaatgta tcaggttatt gcgcaatcca ttgattctga
    26401 aacattgaga gctacagcaa cagatagtta taatgatgtc tttactacac tggctgtatt
    26461 gctttcagca gcaatcgaat gggtaactgg atggcgtatt gatggatata taggttttgc
    26521 tttagcatta tatatccttt atagtggatt gacgatgctt cgaaattttg tatacgaact
    26581 attaggaagt cgcccaacgc cagcagagat tgaagaaatg gaagagcatt taaatgcttg
    26641 cgatgatata cttggctatc atgatttatt ggtgcataac tatggaccca ataaacgctt
    26701 tgcatcagtc cacatcgaag tagatgaatc aatgaatttg aatgtggctc ataagattat
    26761 tgatctgata gaaaaagact ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga
    26821 tccaatgcct gttgataacg aaaaatacta taatatcttg cggcagttaa agcaaattct
    26881 tcttgaaaca ggtacagggc ttaatattca tgattttaga gtaattcatc aaggaaaaac
    26941 gctgcaattt gatgtagtgg tccctgagaa attccagttt gttgatacag aactagaaag
    27001 aatcttacgt agtcaaatag aagaaaaaat cggagattac caattggaaa ttacgtttga
    27061 tcacaattat cttttatatt aaatgagaaa ctataaaaag actccttaaa gtttttaatt
    27121 taaagagtct ttttttcttt taattgttta tggagagcct gacgtgcaag attatctgct
    27181 cctttatttt tgttttcggg caaccattgc aggatcaata aagaaaattc ggatagaagt
    27241 tcttggattc tttgaaggta gggaaggaag agctcgttct tcgttgcgtt tttgtcgacc
    27301 gtagaaacta gtgttttgct gtctgaaaaa cagaaaatcg tttcattctg taagtcgttt
    27361 ttctttagaa attctaaagt ttttaaaaag acttcgaact ctgcttgatg gtttgtccct
    27421 atagaaagcg ggatagatag ctgttcttgt ttaccttgat agacaatcaa gattccacct
    27481 ccactaggac cagggttgcc ttttgtagag gcatcaatgt atacttttaa cataaaaaga
    27541 gattctcctt tttgttcaaa ctaatgttat ggtaagatta tatcattact gaatcggtga
    27601 gaggttagag aatgaaaaaa aatattcgat ggcagcctga actggcacat acgatcatct
    27661 attggtcatt gaccttgatc attctgtttt ttagtctgat tctttcttta gaaaacacag
    27721 gtccttattg gaaaagcaat tttgttttag gtatttttct tgtttttcta tttctaggtt
    27781 tgcggcgttc ttttatttta aaatcaaact ctgtgattat acgttatgct gctttttgga
    27841 aagaccgaga aatatctgtc ggacagattc gatctttgac gccaactaat actggggtgc
    27901 aaatgatgtt gcaagatgag aaagccccat ctgttttcat gatgcgaaaa aaagatcaag
    27961 tagtcctttt tgatcatttt gaaaaaaacg gacccaaatc agaatcaatt ccttatcctt
    28021 atacgaaaaa aagaaaaagc taattttaaa aatcagaacc taaaaaaacg aatttttaca
    28081 ggaaaagaaa agagtgagac gtacggttca tcccgtttag cgtctcactc tatttgttta
    28141 tctattcatg catactagta tcggtagtct gaacgttgcc tttcttgaca tgggcagctt
    28201 gtgttcctcg attcccttcg atgatctcaa attcgaccac ttgattttct tccaaagttt
    28261 tgaatccctc ttcttcaatc gcagtaaaat gtacaaaaat ttcttctgtg tcatcataac
    28321 tgataaagcc gtatcctttt ttgttgtcaa accattttac aattcctgtg gtcaaagcac
    28381 tcacgacctt tctaaagtct gatcatagcc ttcattataa agagtattta taagtacgtc
    28441 aaataaatta aaaacctgta attttatgtt ttttttctga ataaaaaaag gtataattgg
    28501 catatcttaa caaagaagat attttgttga ataaagttcg tgaaaagggg taatcaaatg
    28561 aagaatgatc tgcaaatttc acaagaaaca gagctagaac cgattgttga agtggctagt
    28621 aaattagatc ttaaggaaga cgatttggaa ctgtatggga aatataaggc gaaaatcaat
    28681 ttttctggaa tcaatcgatc aaaggataac gctgaggggc atttgatctt agttacttcc
    28741 atcaatccaa caccagctgg cgaaggaaag tcaacgatca ctgtagggtt aggagacgcg
    28801 ctcaataaaa tcaataaaaa atctgttatt gcattgcgag aaccctcact tggtcctgtg
    28861 atgggaatca aaggtggtgc tgcaggcgga ggatatgcac aggtattgcc tatggaagat
    28921 atcaacttgc attttactgg tgacatgcat gctatcacaa cagctaataa tgcattatct
    28981 gctcttttag ataatcatat tcatcaagga aatgaattga acatcgatgc ccgccgagtg
    29041 atttggaaac gggtagtcga tctcaatgat cgtgaactgc gtaaagtaat tgttggcttg
    29101 ggtgggccaa tccaaggtgt tccaagagaa gatgggtttg atatcacagt tgccagcgaa
    29161 atcatggcta tcttgtgtct tgcggcagac cttgaagatt taaaggctcg tcttgcaagg
    29221 atcgttgtcg gctatacatt tgaccgtcag cctgtgactg ctggagactt gaaagcagaa
    29281 ggcgctctgg cattactgct gaaagatgca atcaagccga atcttgtaca aacaatttac
    29341 ggaacaccag cttttgtcca cgggggaccg tttgccaata tcgctcatgg ctgcaatagt
    29401 attttagcta ccaagacagc attgagatta gcagattacg ttgtcacaga ggcaggattt
    29461 ggtgcagatc ttggcggaga gaaattttta gatatcaaag tacctaactt gaaaaaagcg
    29521 ccggatgctg tggtgattgt cgccactgtc cgtgcattga aaatgcatgg cggaatgaaa
    29581 aaagatgaac ttaaaaatga gaatcttgat gctttgaaag taggttttgc aaatttaaag
    29641 cgccatatac gtaatatgga acaatatcag ctacctgtga tcgtcgcaat caatgaattc
    29701 gtaacagata cagacaatga actcacactg ttggagcact tgtgtgaaaa tcaggggatt
    29761 cttgccaaga gagcgagtgt atgggcagat ggagcagaag gcggcgttga tttagcaaaa
    29821 gctgtagtac gattgattga tagaaaagaa gctaattaca agccattata ccgcctagaa
    29881 gaaacgatac aagaaaagac agaaacaatc gtcaagaaaa tttacggagg aaatggtgtt
    29941 gttttcagca aaaaagcgca aaaacaaata gaagagttta cgaagaatgg atgggacaaa
    30001 ctgccgattt gtatggcaaa aacacagtat tcattttctg atgatccttc actgctaggt
    30061 gcaccggaag atttcaccat cacgatccgc gaaatcatcc caaaattagg ggcaggattt
    30121 ttagtagcct tgactggaga tgttatgacg atgcctggtt tgcctaaaaa acctgctgca
    30181 ttgaatatgg acgtgacaaa tgacggagaa gtgttgggat tgttttaagc ttgattataa
    30241 tctaccatcg aaggagctgt attttgacta cagcttcttt ttttagacga tagggaatta
    30301 attttgttta cactaggatc attctttttc tttattgtat tttgagctat actaaagcat
    30361 aataggaggg tatgatgaaa acgacattta taagaaaaac cggtttttac ggtatgggta
    30421 gctcgattca gctaagaatc aacaatgaaa aacgcagtgt tctcagtcat gaacaaaaga
    30481 taacgcttga tctggaagtg ccctttacga tgcaggtcac cttttattgg ctaaaaagcc
    30541 ccgtttatac agtgaaagaa gcaaagcctg cttatgtgat cacaatgaat tacttgattc
    30601 ttcaaatcta tccgttttta tttctagcaa ctgggttggg ggccgttttc ctacaaaatt
    30661 tatttttcag tttttttgca gttattgtgt tgattgcgtt ctttttctat ataaaaaacc
    30721 gggcatattt gatcaaggag gaaaattatg gcaaactctg atcttttttt aatcagtttc
    30781 aatcgaatcg aaaaatggat gagagacgag atgggaaatg cacgaaatat gggatttacc
    30841 gaacttgtca gacgcctagc tcaaagaaaa caattaatga tcagaaaata cgaagatgat
    30901 cttttacagc ttgctcagtt aaggaatgcg atcgtccatg atcggatcgc agtcgatttt
    30961 gtcattgcag agccaaatga ttggacagtc aagcgaatcc agctgatcga acaagaactg
    31021 attcagcctg aaaccgtgct gcctcgattt gcaaaacgtg taacaggttt tgaacaagat
    31081 cttccgttac ttgaactgtt aaagatagta gcagaaaaaa ggtattcaca atttccgtta
    31141 tacaataaag gaaaatttgt agcattgatt actttacgta atatcgggtt ttggctagca
    31201 aaagaaagcc aaaaaggtcc ggttgattta acaaataaac gagcgttgga tttgatcatc
    31261 caaaacggaa agtacacaaa ttatcatttt gttccagcta aaacacatat ttacgaggtt
    31321 gaagcgatgt ttcgtgagca agggactttg gaagccgtac ttatcactaa agatgggaat
    31381 cccgatggta atctgctcgg gatcgtacga cctcgtgata tttataaaca aatagaaaag
    31441 gattgatttt ttgttaatta tttattggat attcagcgct ttgctagtag gagtggatca
    31501 gttggtcaaa tggtggatca ctgataatct agcgcttgga gagacgaaaa atcttatacc
    31561 aggtattttg tcactgaatc atattcgaaa cactggagcc gcatggagta tgctagaagg
    31621 gaagatgtgg ttctttacta tagtgacact cattgcagtc gttgtgattc tcacattgat
    31681 gattaaaaat cgtgaaaaag gtaatcgctg gttcatgatt ggattgagtt tgattctagc
    31741 tggtgcgatt ggcaatttta tcgaccgtat tcgtcttggc tatgtagtag atatgttcca
    31801 gacagatttt atgagctttc cgatttttaa cgtagcagat gtgacacttg tatgtggagt
    31861 gatttgtatg ttaatttata ttattttaga tgaaaaggat caaagaaaaa aatgacagaa
    31921 cttaaaacga cgattcacca tgaatctggt aggatcgata aagtattgac taatttattc
    31981 accgattaca gtcgttcgca aattcagctg tggctgaaaa atggagcggt ttctgtcaat
    32041 ggacagccag tcaaagcaaa ttacaaagta aaacaaggag acgagattac tgtcttgatc
    32101 cccgaaccag aaacattaga tgtggtccca gaaaatatcc cgttgacgat cgtttacgaa
    32161 gatgaagatg tagcggtgat aaacaaacct caaggaatgg tcgttcatcc ttcagcaggt
    32221 catacgcatg gcacaatggt caatgcgctg atgtaccata tcaaggatct ttctacgatc
    32281 aacggcgtta ttcgtccagg tattgttcac cgaatcgaca aagatacttc cggactgcta
    32341 atggttgcca aaaatgacca ggcacacgaa tcattagctc agcaattaaa agataagacc
    32401 tctttgcgca aatatgttgc tttagtccat ggagagattc ctcacgaaaa aggaaccatc
    32461 aatgcgccaa tcggccgttc gaaggaaaac cggaaaatgc aggcgataag agaagatggg
    32521 aagtcggctg tcacacattt tactgtattg gaacgtttcg aaggatttac tctggttgaa
    32581 ttaaaactag aaaccggaag aacacaccag atccgtgtgc atatgaagta tatcggttat
    32641 ccgttagctg gagatccagt atacggaccg aagaaaactt tgaaaggcaa tggtcagttt
    32701 ctccatgcgc aaatgttagg ttttacgcac ccgactacaa acgaaaagat gatctttgaa
    32761 gcacctcttc cagaagtttt tgaaaaaact ttagaaaaat taagaaaaaa cattgcattt
    32821 taatttagat aacggtatag taattaacaa cgaaagcata aaacttaata gaattccttt
    32881 aagtttagtc ccgtgaggct aagaagggtg ggagcaagga tacgtatggt gcgccataag
    32941 tagattgctc attgacatag tatagctatc agtgtatcag tatacgctca gtctaaacat
    33001 tcctcctgtc ctcttagggc aggagttttt ttatgcaaaa aagtaggggg aatgaaaatg
    33061 caagcaaaag aagtagtaga tcaggtaacc atgaagcgag cattgactcg aattacttat
    33121 gagattatcg agagaaacca tagtatccaa gatatcgtat tggtaggtat caaaacacga
    33181 ggaatctaca ttgcatcaag aattgccgaa cgcctgaagc agctagaaga tatcgatatt
    33241 cctgtaggag aactggatat cactttatac cgagatgaca aaaaagaaaa tccagaagaa
    33301 ccggaacttc attcttctga catcccagtt tccttagaag gaaaagaagt tattttgatt
    33361 gatgatgttt tgtatacagg acgaacgatt cgagcagcaa tggacgcagt aatggatttt
    33421 ggcagaccaa gaaaaatttc attagcagtc ctggtcgatc gtgggcatcg agaattgcca
    33481 atccgtgcag attatgttgg gaagaacatt ccaacatctc gggcggaaga gattttagta
    33541 gagatgcaag agcttgatgg acaagatcgc atcatgattt tgaaagagga agattagagg
    33601 agagagaaac atgtcggaga aaaaaggatt tcataatgat gacgtggtgt tagatatcca
    33661 cgatcgccca accccaggac attgggtagg attaagttta caacacttgt tcacgatgtt
    33721 cggagcaacc gtgttagtcc ccatcttagt aggtatcgat ccaggtattg cgctagtcag
    33781 ttcaggacta ggtacattgg tgtacttatt tactactaaa ggaaagatac ctgcgtactt
    33841 aggaagcagc ttcgctttca ttgcagcgat gcagcttttg atgaaaacag atggttatcc
    33901 agcgattgca caaggagcag tcacaactgg attagtctat ttgattgttg cgctgatcat
    33961 caaaaagatc ggttcagcat ggctggataa aatcttgcct ccaatcgtcg tcggtccagt
    34021 catcatggtt atcgggttgg gacttgcggc aaatgcagca acaaatgcta tgtataatca
    34081 gggcacgtat gatttcaagt atattgctgt agcgctgatc acattaggat taacaatttt
    34141 cttcaacatg tggctaagag gttttctcgg attgattccg attttactag gtattattag
    34201 cggatacatc gtagccttgc tgtttggaat cgtggatact aagccaatcg tagacgctgc
    34261 ctggtttgct gttcctaact ttgagattcc ctttgtacaa tatgaaccaa agctatatct
    34321 tggagcaatc acaacaatgg cacccatcgc ttttgtgacg atgacagagc atatcggcca
    34381 tcttatggta ttgaataaac taactaaacg taatttcttt gaagaaccgg gtcttcataa
    34441 gacgttgaca ggtgatggat tagcacaaat cgtggcagcc tttgtcggag gtcctcctgt
    34501 aacaagttac ggtgaaaaca ttggtgttct ggctatcaca cgtgtccata gcgtgtttgt
    34561 gatcggtgga gctgcagtat ttgcagtcat tttaggattc gtaggaaaat taagcgcttt
    34621 gattttaagt atccctggac cagtcatctc aggaatcagt tttatcttat ttggagtaat
    34681 tgccgcaagt ggaatgaaga ttttaatcga gaacaaaatc gattttgata aaaagaaaaa
    34741 tctgttgatt gcttcagtta tcttagtagt aggaattggc ggactcgtct ttgaagtagg
    34801 cacatttaca ttgtcggcaa tggcattagc aactgtactt ggcatcattt taaatcttat
    34861 cttaccagaa acttcacgaa gcgaagaaaa ctaaggagga aacattatga tcatcaaatc
    34921 agaacgtatc agcttaaagc atttattgac cgttgaggca cttactgatc aagaagttat
    34981 gggcttgatc cgcagagggc aggaattcaa gcaaggtgct aattggactc cgcaaaagcg
    35041 ccagtacttt gctacaaacc tctttttcga aaatagtact cgcacacata aaagcttcga
    35101 tgtagcggag aaaaaactag ggctagaagt gattgaattt gaagcaagca caagctctgt
    35161 ccaaaaaggg gaaacactat acgatacagt actgacgatg tcagcattag gcgtagacgt
    35221 agcggttatc cgtcatggtg atgagaacta ttatgacgaa ctgatacaaa gtaaaacaat
    35281 ccagtgttcg attatcaatg gaggagacgg aagcggacag catccaaccc aatgcttgct
    35341 tgatttgatg accatttatg aagaatttgg tcattttgaa ggtctgaaag ttgccatcgt
    35401 aggtgatatc actcactctc gcgtagctaa atccaatatg cagatgttaa aacgtctagg
    35461 cgcacaagta ttcttctcag gtcctagaga atggtacgac gatgaattcg aagtgtacgg
    35521 acattatatg ccattggatg aactactaga tcaagttgat gtcatgatga tgcttcgggt
    35581 gcaacacgaa cgccatgatg gaaaagaaag tttctcaaaa gaaggctacc atcaggaaca
    35641 tgggttaaca gttgagcgtg cgaagaagtt gcaaaaacat gcaatcatta tgcacccagc
    35701 accagttaac cgtgatgtag aattagctga ttcattagtg gaaggattac agtcgcggat
    35761 cgttcagcaa atgagcaatg gagtattcgt cagaatggca atcttagaag cagtgctaaa
    35821 tggaaaagcg taattatagg aggatgaaaa aatgaaaaca ctcattaaaa acggtcagat
    35881 caatacacga aaaaatatga cgacaccagc agaaatttgg atagaagatg ggcgaatcaa
    35941 agcaatcggt acaggattct cagaagccga atttgatgaa gtgtttgatg caaagggaca
    36001 attgatcaca cctggtttag tggacgtcca tgttcatttg agagagccag gatttacata
    36061 caaggagacg atcgaagctg ggactcgctc agctgctcgt ggcggtttca ctactgtatg
    36121 cgcgatgccg aatttaaatc ctgtacctga tacggcagaa aagctgagac aagtatatga
    36181 catcattaga aaagatgcag ttgtgaaggt attgcagtat gcgccaatca cagaaaatct
    36241 gcgaagtgag aaattagttg atcaagaagc actgattgaa gaaggtgctt ttgctttcac
    36301 aaatgacggt gtaggtgtcc aaacagctgg gacgatgtat ctggctatga aagaagctgc
    36361 aaaaaacaat aaagccctag tagcccatac agaagatgaa tctttattat ttggcggcgt
    36421 tatgcacgct ggcaagaagg cagaagaact tggtcttccc gggatcttaa gcgtaaccga
    36481 gtcttctcaa attgctagag atctgttgct agctgaagca actggtgttc attaccatgt
    36541 ttgccatgtt tctactaaag aaagtgtccg tgtaatcaga gacgcaaaaa aagcaggaat
    36601 ccacgtaaca gcagaagttt caccccatca tcttattttg atcgacgaag atattcccga
    36661 agatttcggc ttttggaaaa tgaacccgcc gctaagagga cgggaggaca gagaagcttt
    36721 gatcgaaggc ttgcttgacg gtacgatcga ttgcatcgct acagaccatg ccccccatgg
    36781 attagaagaa aaaagccaaa gtttcatgaa atcaccattt ggtatcgtag gaagcgaaac
    36841 agcgtttcaa ctgatctata ctcattttgt agaaactggt cgattcacgt tggaacaagt
    36901 gatcaattgg ttggcagtta aaccggctga gat
//
LOCUS       DACEZN010000010        36569 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEZN010000010 DACEZN010000000
VERSION     DACEZN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.010"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IXH60_000372"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IXH60_000372"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1989340.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IXH60_000373"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IXH60_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1989341.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IXH60_000374"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IXH60_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1989342.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IXH60_000375"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IXH60_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1989343.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IXH60_000376"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IXH60_000376"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1989344.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IXH60_000377"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IXH60_000377"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1989345.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IXH60_000378"
     CDS             complement(8054..8524)
                     /locus_tag="IXH60_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1989346.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IXH60_000379"
     CDS             complement(8570..8995)
                     /locus_tag="IXH60_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1989347.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IXH60_000380"
     CDS             complement(9062..9331)
                     /locus_tag="IXH60_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1989348.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IXH60_000381"
     CDS             complement(9342..10937)
                     /locus_tag="IXH60_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1989349.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IXH60_000382"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IXH60_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1989350.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IXH60_000383"
     CDS             complement(14490..15050)
                     /locus_tag="IXH60_000383"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1989351.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IXH60_000384"
     CDS             15403..16377
                     /locus_tag="IXH60_000384"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1989352.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IXH60_000385"
     CDS             16547..17947
                     /locus_tag="IXH60_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1989353.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IXH60_000386"
     CDS             complement(18025..18747)
                     /locus_tag="IXH60_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1989354.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IXH60_000387"
     CDS             complement(18826..19332)
                     /locus_tag="IXH60_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1989355.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="IXH60_000388"
     CDS             19455..21254
                     /locus_tag="IXH60_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1989356.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="IXH60_000389"
     CDS             complement(21322..22896)
                     /locus_tag="IXH60_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989357.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IXH60_000390"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IXH60_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1989358.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IXH60_000391"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IXH60_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1989359.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="IXH60_000392"
     CDS             complement(24673..25311)
                     /locus_tag="IXH60_000392"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1989360.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="IXH60_000393"
     CDS             complement(25312..25962)
                     /locus_tag="IXH60_000393"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1989361.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IXH60_000394"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IXH60_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1989362.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IXH60_000395"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IXH60_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1989363.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="IXH60_000396"
     CDS             complement(29105..29845)
                     /locus_tag="IXH60_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1989364.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IXH60_000397"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IXH60_000397"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1989365.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="IXH60_000398"
     CDS             complement(31216..32154)
                     /locus_tag="IXH60_000398"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1989366.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="IXH60_000399"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="IXH60_000399"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1989367.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IXH60_000400"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IXH60_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1989368.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="IXH60_000401"
     CDS             complement(35314..35619)
                     /locus_tag="IXH60_000401"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1989369.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IXH60_000402"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IXH60_000402"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1989370.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6707 g        11988 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaata
//
LOCUS       DACEZN010000011        35643 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEZN010000011 DACEZN010000000
VERSION     DACEZN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.011"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..631)
                     /locus_tag="IXH60_000403"
     CDS             complement(<1..631)
                     /locus_tag="IXH60_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989371.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNL"
     gene            1319..1477
                     /locus_tag="IXH60_000404"
     CDS             1319..1477
                     /locus_tag="IXH60_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1989372.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            1549..2769
                     /locus_tag="IXH60_000405"
     CDS             1549..2769
                     /locus_tag="IXH60_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1989373.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKTVFEAFQKGLKN"
     gene            complement(2862..3254)
                     /gene="rpsI"
                     /locus_tag="IXH60_000406"
     CDS             complement(2862..3254)
                     /gene="rpsI"
                     /locus_tag="IXH60_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1989374.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3270..3713)
                     /gene="rplM"
                     /locus_tag="IXH60_000407"
     CDS             complement(3270..3713)
                     /gene="rplM"
                     /locus_tag="IXH60_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1989375.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4232..4699)
                     /locus_tag="IXH60_000408"
     CDS             complement(4232..4699)
                     /locus_tag="IXH60_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1989376.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4819..6357)
                     /locus_tag="IXH60_000409"
     CDS             complement(4819..6357)
                     /locus_tag="IXH60_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1989377.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6437..7123
                     /locus_tag="IXH60_000410"
     CDS             6437..7123
                     /locus_tag="IXH60_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1989378.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7324..7851)
                     /gene="msrA"
                     /locus_tag="IXH60_000411"
     CDS             complement(7324..7851)
                     /gene="msrA"
                     /locus_tag="IXH60_000411"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1989379.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7935..8612)
                     /gene="pgmB"
                     /locus_tag="IXH60_000412"
     CDS             complement(7935..8612)
                     /gene="pgmB"
                     /locus_tag="IXH60_000412"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1989380.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8609..9739)
                     /locus_tag="IXH60_000413"
     CDS             complement(8609..9739)
                     /locus_tag="IXH60_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1989381.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9739..11922)
                     /locus_tag="IXH60_000414"
     CDS             complement(9739..11922)
                     /locus_tag="IXH60_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1989382.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIAPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11919..12944)
                     /locus_tag="IXH60_000415"
     CDS             complement(11919..12944)
                     /locus_tag="IXH60_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1989383.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12989..13816)
                     /locus_tag="IXH60_000416"
     CDS             complement(12989..13816)
                     /locus_tag="IXH60_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1989384.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13813..14880)
                     /locus_tag="IXH60_000417"
     CDS             complement(13813..14880)
                     /locus_tag="IXH60_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1989385.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14906..15742)
                     /locus_tag="IXH60_000418"
     CDS             complement(14906..15742)
                     /locus_tag="IXH60_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1989386.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15739..16608)
                     /locus_tag="IXH60_000419"
     CDS             complement(15739..16608)
                     /locus_tag="IXH60_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1989387.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16678..17961)
                     /locus_tag="IXH60_000420"
     CDS             complement(16678..17961)
                     /locus_tag="IXH60_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1989388.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17975..19660)
                     /locus_tag="IXH60_000421"
     CDS             complement(17975..19660)
                     /locus_tag="IXH60_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1989389.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19935..20960
                     /locus_tag="IXH60_000422"
     CDS             19935..20960
                     /locus_tag="IXH60_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1989390.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21555..25208)
                     /gene="rpoC"
                     /locus_tag="IXH60_000423"
     CDS             complement(21555..25208)
                     /gene="rpoC"
                     /locus_tag="IXH60_000423"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1989391.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(25287..28904)
                     /gene="rpoB"
                     /locus_tag="IXH60_000424"
     CDS             complement(25287..28904)
                     /gene="rpoB"
                     /locus_tag="IXH60_000424"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1989392.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29632..30600
                     /locus_tag="IXH60_000425"
     CDS             29632..30600
                     /locus_tag="IXH60_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1989393.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30618..31523
                     /locus_tag="IXH60_000426"
     CDS             30618..31523
                     /locus_tag="IXH60_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989394.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31520..32326
                     /locus_tag="IXH60_000427"
     CDS             31520..32326
                     /locus_tag="IXH60_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1989395.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32483..33451
                     /locus_tag="IXH60_000428"
     CDS             32483..33451
                     /locus_tag="IXH60_000428"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1989396.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33768..34292)
                     /locus_tag="IXH60_000429"
     CDS             complement(33768..34292)
                     /locus_tag="IXH60_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1989397.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34579..>35643
                     /gene="scm"
                     /locus_tag="IXH60_000430"
     CDS             34579..>35643
                     /gene="scm"
                     /locus_tag="IXH60_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1989398.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        10249 a         7526 c         6604 g        11264 t
ORIGIN      
        1 ttagatttcg attcaaaaga ataatattat caaaatactt ctttaccttg cttaaatcat
       61 gatgcacaat tagcactgtt tttccctgat gtttcaattc ttttaacgta tccataatta
      121 ttcgttcact aactaagtct atccctacaa atggttcatc taaaaatatg aaatccgcat
      181 tttgagcaag acatcttgct aaaagtacac gttgaaattg tcctccagat aattctccta
      241 tttgatgatt agaatatttt cccatatcga ccttttccaa agcctttgaa actctttgcc
      301 attctgcatt tttaattctt tgaaaaactt tcattccggc atatgttccc atagatacac
      361 attcttttac ttttattgga aaagtaaaat caatatcagc tttttgctct acatatgcaa
      421 tcttcaccag ttttttattg atatcttcat tattaatgga gacctctccc tgatgaggga
      481 taatatttaa cattgcttta agcaatgtag attttccagc accatttgga ccaataatac
      541 ctgttaaagc accttcttgt atttttaaag aaatctcgtt caatgctaaa aaatcgttat
      601 aacaaacatt aagctttttc acttctaaca taactttctc cttctaaatg ataatgatta
      661 tcacgatcat atggtataca aaaattatat tttagtcaac cctaaatttt ataatttaga
      721 tttttttctg ttgctgaaga ttagaatgac aaaactattt gaaactacgc gtatagtaag
      781 tagctttcaa aatatataaa aaaggagcgt tctcaaatga gaacatgctc ctaaaactac
      841 tttattggcg tatggaaaaa ttatacttaa agtggatagc gggttgaata agttgataag
      901 catcaaaaca tttataaaaa ttattcaaga ctatgtctgt tccatataca aatatgaaaa
      961 tcagtaaact ccatttaact tttttcgtag tgtgtctttt tatacatgtt tatcgttatt
     1021 aatagtgaat aggaaaaaac ctatttttga tcattgacaa ttaaatacaa tttttgaata
     1081 acgatccctg ctattcgagc tagaaactca agtacgccat gacctgatta gcgacaaata
     1141 cttagagatt tctttttgtt ctgggaaaac aaaatgccat gacaatagca gcaaataatg
     1201 atacttccgt aaggctcgcc gtaaactggg gagaggtgaa attcctatgt gcttacccaa
     1261 agcaattcaa aaattctaat cttttttagg agatgatttt ataaaattga gagtctactt
     1321 gaataaaaaa caaacctgtg agtttctagg aattgataat aatactttag ataagtggat
     1381 tcgtatatat aattttccag taatcagaat tgacggtata gttcgctttg acatagagga
     1441 tatcaaagat tttatgaata catttaaaac aatttgaaaa tttcttctcg ccgcatatac
     1501 taagaatagc gaaatatact gccttacttt ttaagggagt gaggaatttt gcctacaaaa
     1561 ctttcaaatg ggaaatataa aactaatctg aggtatccaa aaaaattcag agaaatcact
     1621 ggaattacat cagaaaagta tcaaaaagta tttccaacta ggcaattggc tattaaagcc
     1681 gaaaatgcaa tgaaaaggaa aattgaaaca gttttaagag aggagaatgc aaattcacta
     1741 gaattaaaag gaaaaataaa atttaaggaa ttctatgaat caaaatggct tcctagatat
     1801 gaactaggtc agactactag atcaaaaaga gctccaagtt acgtaacaat tagtaatact
     1861 aaagatatat tccgattgca catccttcca atgtttggtg agtatgcaat gaactatcta
     1921 aattcaaata ctgagataat atcagatgaa ttaacaaaaa aatcgaaaga atacgcaaat
     1981 ataaaaatta tcaaaggata tgtccgttct atgtttgata ttgcagaaat actaaattat
     2041 attgaattta accgaactac caaaattatt caatgtatca ctgcacctaa aaaaattgct
     2101 ttagaagaaa gaaggatacg ggaaggtaat caagcattat cgtcagaaga gcttattgct
     2161 tggatagaag cagtaaacac tgacttgaat aatcacttgc ttactcttca tgactatact
     2221 cttttcatgc ttaccctata tcttggtgat agaaaaagtg agacgtatgc tttgcaatgg
     2281 aaatatatcg actttgagaa acaaacggtt cgccttaaac atgctttgga taaatatcaa
     2341 aaaaagaaat ttacaaaagg tcgaaaggat accgtaattc aagtacccga aattgtaatg
     2401 gcattactaa gcaaatggaa atcagtgcaa gctgagcaat tgttgagatt aaaaattaat
     2461 caaacaccag atcagtatct ctttacctat actaaaccat ccggagaagt aaattgccct
     2521 gttcacgctg attacctaaa ctatcgaatc aattcaatta aaagacgaca tcctgaattg
     2581 gctcatttat ctccacataa gcttcgtcat acctatgcaa ctatagctag acaaggcgga
     2641 gcaaatatga atcaaatatc taatgcttta acacactcag atatttctac tacaaagata
     2701 tacgtaaaca caccagatgt agttgataag actgttttcg aagcatttca aaaaggatta
     2761 aagaactagg atgaatgtgt tctcgaatgt gttcttaaac taaaaaaaga cactttccaa
     2821 ataattgaaa agtgtcttga aacattgata tacaaagaat attaacgttt tgaaaactgt
     2881 gaagctttac gagctttttt aagacctggt tttttacgtt cgaccatacg tgcgtcacga
     2941 gtaagtaagc cagcacgttt aagagctaaa cggaagtcag gatcaacttc tagcaatgca
     3001 cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc gtttacgtta
     3061 acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt gattacttca
     3121 cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt aattttgcca
     3181 gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt gccgatatat
     3241 tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc taaaacttct
     3301 ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt tgtaaattgt
     3361 ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat caaacgacga
     3421 gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg gtagttgctg
     3481 tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt gtctgcattg
     3541 atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt atttttacca
     3601 cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt tgcgtctact
     3661 acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg cacgttattt
     3721 tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc tctttgtggg
     3781 gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg tttttctgct
     3841 tgattgaaaa atttatatga aactttactt cttttctttc ttttatataa aagggtcgtt
     3901 tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag tcttgtcaca accccttatt
     3961 ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccataaaaca
     4021 taagaagctg ttttttaaaa ttgtttcttg tgactcggag ctgaacggca ggcttacaac
     4081 ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
     4141 atgaggaact attttgacgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
     4201 cctctttttc ggtcaagttc aacaacagtt tttacgcgtc gatctctggc gctttgccca
     4261 aatgagcttg gatcatccag atacgttttt ctgtggctgt tttgaattcg ataaagatat
     4321 cttgtgttgg atcgtcgcct tcttctccag aaacttcgat tcctttttgg taaagatctg
     4381 ctagataacg atagcctgca acaagtgttt ccaaacgttc tggaatagta cgatcccagc
     4441 ggccaatttc atcagggatt tttgtgttat ctgcaaattc acgcaatgta gaataaggtg
     4501 aaccgtctaa tgtgatcaag cgttcggata cttcatctaa ctgagcgttc aaatcatcca
     4561 tgaactcatc catcattggg tgcaatgtaa ggaaacctgg tcctctcatg taccaatgtg
     4621 tttggtgaac aacaacagac atttggctta gatctgcaac taattgattt aatacttctt
     4681 tggtttgttg gaatttcatc tttcttgcct cctaacattt tcttacactt aaaataatag
     4741 tagactttca atgaaaagta caacaaaatc atttacctga gaacgatttt cagttcgctt
     4801 ttttatccgt aaagccaatc atttttcaga atcagctaat tcttctcgca gttgagcaaa
     4861 ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca aataatggat agattttctg
     4921 atatttttta tgatcagttt cttgtactcc aacttgctct ttcaattcta tcaattcctg
     4981 tgcttcactg atactttgga tctttccgat gcttctccag cctaaaatca cagctcctaa
     5041 tgcggatgct tctgtgacag tagggaaaac aagcgggtaa cccagtacat cagccatcat
     5101 ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca cggatctctg tgagctgatt
     5161 ttcctggcaa actcctttta aaatccgata aaggttgaga cagatccctt ctagaatcgc
     5221 cttcatcatt tctttttctg tatgttgccg ctgtaaaccg ataaaactgc caaacgcatc
     5281 tgcgtcccaa agcggtgctc gctcaccaag taaataggga tgaaaaatca gaccattggc
     5341 acccgctggc acaaaagcga tagccgaaag cagtgcatca tatggatttt ctcctttttc
     5401 tttagcagat tgaacgactt cctgtaaaaa tgtatgccct gcccaatcaa agatgccagc
     5461 accattgcta gtcgctcctc ccactaccca atgtttctga tccagaacat aacaaaacgt
     5521 ctccatttct ggatggaact gaggctgagt agtaataaaa cgaagtgctc cgctcgttcc
     5581 tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac cctagattag aaagcggccc
     5641 gtctgcccct cccaaaacaa atgatatatc atttggcaaa cctagttctg ctgctgtttt
     5701 tgatggtaga cctgttactt gataggtgga aggaaagact tgcggcaatt gctctattcg
     5761 aatgttcaag tagtcaagca cttcttctgc ccattggaat tcatggatat caaaatatcc
     5821 agttcctgaa gctgttccgt agtcgctgat cagttcgcca aacaatctat aaaagatata
     5881 gtctttgata ccgacaaaat aagcagtttt tttgaataat tcaggttggc tgttttgcag
     5941 ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc gtcccagtct gccgataaaa
     6001 ttctttgcca agcacggtat atttcagttt ttctgcttcg tccgctgctc ggttatctgc
     6061 ccaagtgatc attcgagata acggtgtttt ctcttcatcc aacaatatca ggccatgcat
     6121 cgcagaggag aaggagacaa attgaatgat tgtgtctgaa ggcaactgtt taattacttc
     6181 ttttataacg gtgcagacac cttgaaagat caattcctga tcttgttctg ccatcccttg
     6241 tgtttcttgg atcaaagggt agcgaaaata agcggatgct gcttcttggg cttcatctgt
     6301 gaaagcaact gctttggttt gtgtggttcc gatatctaat ccgattgaaa cgttcatttt
     6361 ttcacctcaa attttatgta tgtacaaaaa tttagtttaa gcttgcgtat cttggttttg
     6421 aaaaggggga gatggcgtgt tactttattt tatcattggt tgctgtatcg gatcattttt
     6481 atgtttaacc gcccagcgga ttccgttagg tcactctatt atttatccta gatctcattg
     6541 tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt ccgattctct cgatcatcgt
     6601 ccaacgattt cgctgccgct attgcaggtg tcggttgccc ttctactatt tgttggcaga
     6661 agccttatgt ggtggtcttt ttgcctggtt ctttactttt tcttccgcaa gaaatttttc
     6721 gacgttcata tggatgcttt cggctttgct attttcgttg atggatttat tttacttcat
     6781 ggtccattct cacacgctat tctgttcttg ggcaatttta tggatttttt ggttgcaggc
     6841 cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc gtaggcggtg tgtgccttcg
     6901 ctatggtcaa tcctatctcg gagctggcga tactctgttg atgctttctt ggagtggtgg
     6961 cttgtctcta gaagagttgc ttcagatctt gtttcttgct agtctgtttg gaatatccgt
     7021 tttttctgtt tactttatct gtcataaaaa aaagttggaa aagctgcctt tcattccttt
     7081 tttgagcagt gcgttgctga ttgtccttca tcttaggtat tgaaaaaagg ttgtgacaaa
     7141 cgacttgtca caacctctga tatatagtgt tctcttacca aatccttgtg acttggagct
     7201 aggcgacagg ttcacaacct ctccactaaa tggtgttcaa tcagctgacc ctcagtatta
     7261 gctcaaaatg ctggttttac aacctctgat atacggtgtt ccataagtag ctttttcggc
     7321 ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct gcacgtccag aaccttctcg
     7381 ataaagattg taatggagct tgtttttctt gtaataatcc tgatggtaat cttctgcagg
     7441 ataaaattcg cttcttggtt cgatttttgt cacgattggc ttatcgaatc gcccgctgtc
     7501 ttgcaatgct tgttttgaac gctctgcaat atttcgttct tcttcatcat tatagtagat
     7561 cactggacga taagaatcac cgcgatcagc gaactgtcct aatgcgtcag tcggatcggt
     7621 ttgctgccaa tagatttcca ccaactgatc ataagaaata acttctggat catattcgat
     7681 ctctactgct tctgtatgcc ctgttgtgcc tgttgttact tgttcataag ttggatttgg
     7741 tacatgcccg ccagtataac cggatgtcac agaaatgatc cctggctgct cttcaaatgg
     7801 tttgaccata caccaaaagc atccgcctgc gaaaactgct ttttttgtca tataaaatcc
     7861 cccttttttc tcatcatacc ttgatttcca aaaggaggat aacgttttgc ttgtgacaga
     7921 aaaaacggaa actattatat acgtgatacg gcttctgata aatgccaaaa cgaacgtact
     7981 tgatcaaaaa cggttaaatc aggaatcaca tattcagcag aaggaagatt tatgttttca
     8041 cctattccca atacatgcat gttaccagcc aaagctgctt gacaaccagc ttctgaatct
     8101 tcaatgacca agcatgcatt tggcggtaca ttcaattgct gtgctccttt gagaaaaact
     8161 tctggatcag gttttgcttt tgatacttgc gtgccgtcaa taaggacatc aaaataactt
     8221 gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc ttgctgatcc caatccgatc
     8281 ttgatgtttg tttttttcaa gtactcaaga acagctattg caccaggcaa aacggatgtt
     8341 tcatccattt tttgaatcgc ttgaagatat agattgtttt tttgctcagc aaaggctgat
     8401 ttctcttttt ctgagaattt ctgttccatt tttccaaatg acagcaatcg ctctagtgac
     8461 tcggttctgg atattccttt cagttgctcg ttttctttct cagtgaatgg aatggacagc
     8521 tggttagcta aaaccagcca cgcttcgtaa tgataacgag ctgtgtctac aagaacgcca
     8581 tctaaatcga atagtgctgc tttgattttc atcgattacc tccttgaaaa tgaacgactt
     8641 gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt atcagtggct tgctgtaaag
     8701 cttcttgtaa aggcacagct gcaggaagga tcggaaaaac ggccgaaatg ccaacatctg
     8761 ctgctttttc gcttccttta ccaacacttc cgcaaatagc tatcacagga acgccctctg
     8821 gtactcttct agcaatccca actggcgctt ttccttttag actttgtcca tctagacgtc
     8881 cttcgcctac gattacgagg tctgcttgct gtacccgttt atcgaactcc agtaaatcca
     8941 atacgtagtc gatgccagag actattttcc ctgaagcaaa tgctaacagt cctgcaccca
     9001 ttccaccacc agcactactg cccgatgcct cgattacttg aggggcaaat ctcatataaa
     9061 aatcatgcat ggcttggtca gccactgcta attcattttc tttcaagcct ttttgtggtc
     9121 cgaaaacaaa agaagcgcct tcttttccgc ataaaagatt agtaacatca gtaatgacag
     9181 tgattttggc tttaatcagc tcaataggca cgttcgtttt atcaactgta gcaattttac
     9241 ctagattttc tcccagtgct tctacttttt gattatcttg atcgtaaaaa gcataaccta
     9301 gaccttctgc catgccgatg ccgccatcgt tggtactact tccgccaacg ccgatgatga
     9361 tgtgtcgaac accttgttgc aacaattgtc gtatcagttc tcctactcct cgcgtggata
     9421 acctaagtgg ctgttgttgg tttagtggga catctttcaa tccgacgatt tctgccattt
     9481 caaaaatcgc agtttgaccg tctgaagcgt actggatttc tttgtttttg ccaaatgcat
     9541 tagtaacgac tgctgtttgc cgcttcaacc ttaaactgtc tgctaatgat gaaaccgttc
     9601 cttctcctcc atcgcctata ggtagcagat catattcagc ctgaacaaaa acttcttgaa
     9661 aacctgattg aatggcttgt gctgcctcta aggctgtcag actttctttg aaagaatctg
     9721 gcgcaatcag aatacgcatt attttcccct ccgttccaag atcagttctt tttccagctg
     9781 gtattggtct ccattgacaa aaataggtaa tgtttctttc gtctcttttt ttattactat
     9841 ttgttttttt gtcaaaacaa atagcaacct gtgatttttc caaataaaag gaaaagaaat
     9901 tttttcccat gctgaaggaa gtcttggaga aatagaaaga gttccattcg ttactgtaat
     9961 acctgaaaaa ccaaatataa ctgctagcca tagtgctcct aatgaagcag catgaattcc
    10021 gtcatctgat gaatgcggat ctggtcctaa atcaatcagg cacgcttctt ggaataaatg
    10081 atacgcccat tccatttctt ggatacggca agcctctatg gcgtgaatcg ctttactcaa
    10141 cgatgagtcg tggattgttt tgttttcata gtaatgcaaa ttcttagaaa cgattggttg
    10201 agaaaaatac tcaggtaata agtataacag catgacaaga tctgcttgct tgctgacttg
    10261 gagttcattt acttcgctac gggcataatc aagtaaaata gactggctgc caggacgttc
    10321 tttgtaagtg gtcaaatcaa taactggctt actgagatac gtgtcatctt gcggaatcag
    10381 ctgttctttg ttaggcactg gcaaatataa cttatctaaa aaattttgac attctttcaa
    10441 aaaaagcgga tcgccagctt gatatttaac agctaaggcc acattataat gcgccagata
    10501 gttcgtatat gcattattgt cgatatattc tgtgtattca tctggagcaa tgactctgtg
    10561 tatctctaaa tgatcatcga cttttacagc tctgctcagc caaaaagctg ctgtttcatg
    10621 aatgagcgtt tttccctttg tttctaaaaa cgattgatcc tctgttgctt ggacatactc
    10681 ttgaacggca taagcaaggt ctgcactgat atgatgttct gcggcagctg aagcaacttc
    10741 ttgtcttgtt ccggttttga tattcaatgc agcaaatttg ggtgtctctt cctgaccagt
    10801 gtgtgcgctt tcccatggga aaagtgcacc ttgatagtga taatctcttg ctttttgccg
    10861 tgcgccttct aatcgatcat aacggtacag cagcaaattc tttgctactt caggaaactg
    10921 atataaaaag aaaggcagga taaaaatttc cgtatcccag aaaacatgcc ctttataacc
    10981 ttcgccagtt agtcccttcg cccctacact aacacttgga tcattgcggg gagtcataat
    11041 ctgtaaatgg taagcagcga agtccatcgc taactgatcg aaagaagaat cgccttggat
    11101 cgttatccga tgctgatgcc aaaaatctgt ccaataagac atcgactgct ctaaaatttc
    11161 tgtataacta acgcttttct gggcttgggc aatacttttc ttaaacgggt ctgtttgctg
    11221 aagagaggta gagagcacga tgatcttctc cagcgtaaag gacgtatttt ttctgatctt
    11281 ttctgaaaag gaagcggtga tccggcgatt ttttgcttgg aatacacctg ctttattgaa
    11341 tttagctgca atcccaatat caattttact ttctgttgtc tggtaggatg cactgatcaa
    11401 atcgtcacca aatactcgca gttctttttc aaccaactgc tgtgtaccaa aattggtttg
    11461 ctgtccatcg atacccgttt tcacatctac ttggcagtct tcggtcaacg gcttgatgac
    11521 cactttcagg gctgcctgat ggagattatt ttgatcagca acacgacaag taatgacttc
    11581 cacttttttt ccttgagcag tttcaatgag cgcatagcgt ttaagctctc ccatttctaa
    11641 atctagtgcc cgctcgtatg caagaacttt catcgtctca agagaaaaat gttcaccgtc
    11701 gatttttatt tcccatctga tgacatctgg gagattcact aactcggctg attctgctcc
    11761 taaaggtcgg ttatatacac cagctgcaaa cattcccctt acttgattcc gatagtcttc
    11821 ctcctggctt cctctgatac caaaaaatcc atttccaatg gtcatctgac tcgcatactt
    11881 attgatatac tctaaatcaa actgtttttc ttctactttc ataaggtaat aacttctcct
    11941 gtttctgccg aacgatataa attttccacc agattctgga tgacatatcc ttgccagcca
    12001 tctgcaatca tctgagtctg gtcaccgagc gcccgattca caaaatgagc catacttagt
    12061 tcatgtgggt cattaactgg cgtggtcttt tgtaaggaaa ccagctttcc atctacatct
    12121 tcgaatagtt cgcctggata cagattagca cctgcctgat cgccacaaaa agccacattc
    12181 agtttgtctt ctctgatatt caaagcaaac gaagtattca gttggatgat cttgccgttt
    12241 tctaattcca gtgtgccaaa aattgcatct tctactgtga atttgcttgg atcccatttc
    12301 ccgaatttcc ctgaattttt tttcgtacca attttttgga agctgtacgc ccgaacgctt
    12361 tttactttag gaaatccagt aatatacatc gctgtatcaa gcatgtgaat gccataatcg
    12421 atcaaaggcc cgcctccttg aagttctttg tcgataaacg ttccccagcc tggaacacca
    12481 gaccgacgaa gcgcattcgc ttctacgtaa taaatatccc cgagaacatc aagatgattc
    12541 ttgatcattg ttgcttcgtc agaaaaacga tgctggaatc cataagccaa aatctgcccg
    12601 tttttatcgg ctgtctctgc catctttctt gcttgatcag ctgtcatagc tggtggtttc
    12661 tcgcacatca ctgaagcatt agcttcgagg gcagccaata catgttcgaa gtgaaagcga
    12721 ttgggtgtac agacactcac gatatcggga gaaatctcat tcagcatttg tgtaactgat
    12781 gtatacgcat gagaaaatcc tgtacgtcgg caaaagtctt ctgctcgctg gctgactgga
    12841 tcaaccacag cggccaattc taagacatct gatctttttt gataataagc agcatgtact
    12901 ttttctgcta cttgtccagc gcctatgatt gctactttct tcatttattt ctctctcctt
    12961 tgcttattta cttattgtta ttctttctct attctttctc taaatcattt aaatgacgcg
    13021 ctgtttccag acagtttttc aagtattgca atgattcctg ataaattttc acttcatttt
    13081 ctcctcgcaa acgacattca tataccatat aaccttcata ctcattttga agtagcgttt
    13141 gaaaatggcg agtaaaatca attgcgccag atcctggttg gtagcgatga ttatctgcta
    13201 aatggatatg acccacatac tctttttgcg tagtcaatgc ttttgaaata tcatcttctt
    13261 caatattcat atgataaaaa tcagcgatga tctgcacatg tttcaattga ttagagtcaa
    13321 tgtaatgacg cgcatccgct aacaaattga tcatatggtc ttggtatcgg ttcaaaggct
    13381 ccaaataaat aaccgtacct gtttcttcgg ctactttatc taaataacga agtgattcgc
    13441 tgactgcttt atagtctcct tctttgcttc ggggtgaaac catcggcggt aatcgataag
    13501 tgaacattcc ccaagctgct gggataacga ttcctttgcc tccaacctcg cttagtgcac
    13561 gcaaaatctc agcaatttga cgtacaccat taagacgccg ttcttcgata aagtcaccga
    13621 tccacccatc gtagccgtta caagctgtca ctactgggat ccctgtttct ttgattgcct
    13681 gtttgactgt gtcgatattt tctattaaca acttaccgtc gatttcgtat tcttcaaatc
    13741 cacagtcttt gataaatcta aatttttcta aaatgtctct tgggaaaaat gcttgatctt
    13801 gcgtaccgaa tttcattgtt ctcctcctcg gacttgaacg cccattttga tactttcttc
    13861 tgggtgctgg tctacataac gcatgtaagc ttctgcgctt tctgcaaatg gcacaactgg
    13921 atcaatgatt tcttcacaat ccaagtaacc gttcatcaat aattcccaac atgtgtcctc
    13981 gattcgttta cggttccatc ttgggtaatc tgggtttggt tcactagatg ctcgagcaaa
    14041 gacgattttt gcattattga aatgtgcttc gcgaccaaga ttaaacccag ctggaaaagg
    14101 tttagcaaag gcaacataag aaatgatccc gccatagccg atccctatta aagaagcttg
    14161 taatgcggca gcactgccac tcgtttcaat aactgaatct actccacgtt tatctgtcaa
    14221 ttcctttaga cgaaaaccga catctgcttt cataggatct agcgtgtaat cggcaccgtg
    14281 tttttctgct aattctctac gctgcgctat gggatcgatc ccaacgatgg tagtagcacc
    14341 tgctttttta gctaattgaa tcgcaatttg cccgatagcg cctaaaccga agattgctac
    14401 agcatcacct gctcgaacat gtgcttcccg gataccacct aaagcgaatt gagcaggatc
    14461 gtaacatact gcatttttcc attgatccaa ccgatcaagt ttacgtagtt tataattatc
    14521 aacaccgttt gtaatcagcg tttcacgaat gggtccataa gtgcagacta actctccttt
    14581 ttgatactct gtcaccaaac tgcccgtttc ttcgatcgta ccgacaatca tatttcctaa
    14641 ttgaaattcg ccaaatacca caccgcgttg gcttccttct tctcttggca agaataggcg
    14701 ccattcttct gaaaattctt catcgataaa tggacttaat cctctaaagt cgacaatttc
    14761 tgttcctggt tttggagtag cgaaaattac tcggatacgt acttcgttct tttcgatctc
    14821 acggtcttcg taatctttga atccggctat tcttggttct aatgcaacta attttttcat
    14881 cttttctttc ctctttcttt tctttttatc cttttacgcc gccttcgact aaatcacctt
    14941 taataaatcg ttcagataac gcatacataa tgatgactgg taaagcggtc accaatgaag
    15001 cggccatcat tcgtccccaa atgtagtctg gtgtactgaa taatgtgttc aaaccgattg
    15061 gtaaagtaaa tttttcggag gtcgacagga aaatcgaagc aaataaataa tcattccacg
    15121 aaaccataaa gcaataaacg aagacagaaa tgattccaga aatagccaat ggtataatga
    15181 tccgaataat gatctggatc cggctaagac catcgatcat tgctgcttct tcaatatcag
    15241 tcggaatcgt gtcaaaataa ctttttagca tgaatatcgc agtaggaagt gtttgaacga
    15301 ccatcgtgat aatcagtgct gtattggtat catacaaacc tagaccggaa atgattttaa
    15361 ataatggaac aacgatcaaa ataccggaaa acatataaac ggtgtaaaag ctagcattga
    15421 ttttcgagcg agctttgaag cgtagttttg acaaagcgta tgccccgctg atcccaatca
    15481 ctactgcagt tacggaggct atcaaggaga tcactaaact atttttgaaa tacatgatga
    15541 atggaaaaat ctcagcattg aaaatatcct gataatgttc aaaagtccat tctttcggaa
    15601 aaatagtttg tgtcgttgca attgcttctt gtgaatcctt gaatgatgtc atcagcatga
    15661 tcaaaaatgg aaacaaattg acagctaata gcaacaaaat cgtcgcgtaa aataagattt
    15721 tcctgctctt tttatttttc atttcaagtc caccttcttt cgtgagagca agacgaccgc
    15781 tgcaataatg atgaacaata gaatcgatat agctgctgct tttccaagat cattgaacgc
    15841 aaaggctgtc tgatacaaat aaacgcctaa gatattgact ttattcgtca gtagatagac
    15901 atctgtaaac atatagaaca tccagataga tcgtaaaaca atcacagttg ctagtactgg
    15961 tttgattgcc ggcagtgtca cgatcttgaa cttctgccaa gcatttgcac cgtccatttc
    16021 agctgcttca taaagcgttt tgtcgactgt ttgcaaaata gccaaaaatg aaataaacgc
    16081 atacggatag tatctccaga tagcaaacaa aacgaccata aagaagctag caactggctg
    16141 gtcgaaccac agcggtgctt cgttgaaaag ctgcaaccta tctactaaca gatcattgac
    16201 gattccataa ctattattga acatatattt ccaagcaaaa atcaaagaaa tagatggcgt
    16261 aacataactt aataaaatca gtgaacgcac cgttttccta aatctgaact tgcggttaag
    16321 caaaatagct acggctaatc ctaaagctgt actgcctgct acaactaaga atgtgtaagc
    16381 aactgttaaa cctaaagaac gataaaattc actatcactt aaaatttcaa gataattgct
    16441 taatccgata aaaacggcgt ctgagttagg attcagcggc atatcgaaaa aactgatctg
    16501 aatattggaa agcattggaa tcagcactaa aacagctaat aaaagcatgc ttggtgctaa
    16561 aagtcccatg gctaatttta agtcagaatg tttccttgga cgattcattg aaacctcccc
    16621 ctgataatct gccaatagct atctactttt ttcgaacacc attggcagaa cttcttgtta
    16681 tttcgtattt ttttgtgcgt cagctaaagc ttgatcgaca tcttcgtttc cgacagttac
    16741 ttggttcact gcttttccga tcacacctga gcttgtgata tctcccattt tcgtgaaatt
    16801 cttttccccg actagtccaa agacttgtac ttcatcaaaa gcactagcaa tctcagatgg
    16861 cagctcgcca aaagacttga tgacttcatt ttctttgtaa ccatccagtt cgactacttg
    16921 tttattcact ggttgagcac caccaggaga catcaagacc cacttttcca tgtttttcgg
    16981 ttgagataag aaagctgtga atgcttcagc agcctctttt tgtttctcgt ctagaccaga
    17041 agaaatcgtc aatccagata ctgtgccata gacagcttga tctttttgtg ttgggatagc
    17101 aaaaccgata ttttctgagt tccccgcttc ataaacagaa ggcaaaatgt aagttgaata
    17161 gattgccatt ggtgctgttc cattcatgaa agcatctttg atttctgtca catcattaga
    17221 gcctggcatc gtgtattggg ataagttttg ataaaacgac aatgcttctc tcatcttttc
    17281 cgtatcgatc gttacctcgc cttcatcatc caaaacattt gctccatttg acaaagcaaa
    17341 ttgagagaac gcttgctcac tcattgttcc ttcagcagta ggcatagcaa ttccatattg
    17401 tttattctct ttatctgtga aatgttgagc aatttttaaa atatcatccc aattttctgg
    17461 ttctgaaaaa ccagcttctg ataatttttc tttgtcatac caaatacctt gaacccagcc
    17521 gctaattggt gcagcaatat agctttttcc atcttctgaa cgaacaaggt ttttggctcc
    17581 gtcataataa ttatcttctc cagcttcctc gataacgttt tggactgccg tctgatcaat
    17641 cagttcatct ttatccatca ctttagcaaa atcctgactg acttccatta cttctggtaa
    17701 tttaccggaa cgagctaacg taacgatttt tgtattgaac gctgattctt ctaccggcac
    17761 ttgcttgatt ttgatgtctg gattttcttt ttcaaaatca gcaaccagtt cattgatcac
    17821 acttaatcgt tcctgttcga cagaagagtg catgaattga atcgttgtct gttgtgattc
    17881 gttagagctt tgactttcac cacaagcagt aagaagaaat ccagtagcta gcagacaggt
    17941 agtaacaata atttttttca tttttttcca cctcttattt tattattttc agccacatat
    18001 attcatacgg ctggaatatc aactttcgtt cttgactgca atggataatt tgtccagaaa
    18061 acaggtctag gtaatctcct tttggtaaaa gataacctgc tgattgattg gaaaaattat
    18121 ggataactaa gactttctct tccttcgctt cgcgaataaa actgataaaa gaatctgctg
    18181 agtccagtaa gtaaatcaaa ctattcggat ggaaggcttt ttcttttttt cgaatagata
    18241 gcatctgggt caattcacca tatactgctg ttcttaatgg atctgtctgc aactctttct
    18301 caatatctgc taacaaaaat ttttgccgat tgatttttcg attctctcct gttttttcta
    18361 cgcctttgta atcatttcga cttcccagca cactttgaat ataaattgcc ggcacgcctg
    18421 gtaacgttac caaaaatgaa tgagcagcta aaaagcgttt gcttctccat tccgtcgtat
    18481 ctgttggaga acttaacgca tctagatacg tagcgtttat ctcatatgga ctggtcgttc
    18541 catctgggtt tttcttgtag tttactcggg caccctcttt cttcagctgt tccactaatt
    18601 caaggatcgt tttttccggc agaatacctc gtaccggatt caatccgata ccgtcatggg
    18661 aggctaaaaa attaaaaaaa gttgtttttt tcgatgcgaa atcgatggtt tttaaccatt
    18721 ctttcaactt acttgtatta cccgaacgaa tcgcttgaag ggtcaaaggt ggtagcgaaa
    18781 actgatagac catctgtgct tcattctcgc cattcccaaa ataactgata ttatctttgt
    18841 gtggcacatt tgtctcagtg atcaataccg ttcctggagc tgcttcatca gcgattgcac
    18901 ggaataattg gatgatttta tgcgtttctg gcaggtgtat acaagaagtt cctagttctt
    18961 tccataaaaa tcccacagcg tccaagcgaa taaatgaagc gcctttttct aagtaaaaca
    19021 aaagaatatc gatcattttc aataaaactt ctggattttt aaaattcaag tctacttgat
    19081 cttcgctaaa agtcgtccat acgtgttttt ctttcccgtt tttgtcaatg aacgttgtaa
    19141 gtaacgggct ggtccttggt ctagtcacgc tgcttgtatc tgtatccagt ggcaaatcaa
    19201 taaaaaaatc tttgtacgta tcattcaagt ttaaatattc ctgaaaccaa gtactttcgg
    19261 cagacatatg attgcagaca aagtcgaaca ttaaccgtgt ttcgcttctc atttcttcta
    19321 tatcttccca tgtgcccaaa cggtcgttga ttgcctgata atttacaacg gagaaaccat
    19381 catcagatgt atatgggaaa aatggcaaga aatgtacaac tctaaatgta tctgccaaat
    19441 acttttgata aaatttctta aatgtacgca aagttggttg ctgctcttca aaaaattgat
    19501 ccccataagt aatcaagaaa atatcttctt cgtcccaatg cagtttgcct gcctggcctt
    19561 tcttctgctg ataaacactg actttttgtt caatcataga ggcggttttc tcagacaaat
    19621 ctgagtcgcg ataaatcgct tgtaaaagtt ttgtatacat cggattcccc tttctttttt
    19681 gggagcgttc ccaacgattt gatcatatta tggaaaacgc ttactgtcaa tagcattttt
    19741 ttaaataatt ttgatataca caaaaataca aataagaaag cttatgtatc aataattaat
    19801 agcttcttaa aaaaatatgg tctctcatga tcatgcaagc tattcctaaa ttttcaaaat
    19861 catagacaaa aaagaatgtc tattgtacta tggaattatt ctaaaacgct cccaaaaagg
    19921 aggtgcatat aaaattgaaa cctactattt atgacattgc gaaaaaagct aatgtctcaa
    19981 aatcaactgt ctccagagta ttaaataacc agccaaatat ctctgataaa gcaaaagcaa
    20041 gtgtacttca agcaatcaaa gaattgaatt atgaaccgag caaaattgca agaggactgt
    20101 caggaaatgg ttttgacgcg attctagcat tatctcatcg tacttctcat acgacctatg
    20161 gcaatccttt tttttcagat gttattcaat ccatttctgc cgtttctgaa caaaataact
    20221 tcgacttgat tctacaaacg gcaaaaacac cagaagaaga gctagaaaaa tgcgcgaaaa
    20281 agatccaaga aaaaatgatc cgaggaatta ttattttaag ctctacgatg gatgaaacac
    20341 ttctttttga attggatcaa tacaatattc ctattgtggt aatcggaaaa gtcggaaacg
    20401 actacacgaa catctactca gtggacacgg ataatttcca ggatagttac gatttggtgg
    20461 aaacactgat caagaatgga caccgtcata tcggctgtct ccatgctccg acagaagttt
    20521 atgtatccaa tgatcgtctc aatggttacc gtcaatgctt atttgatcac cacctagata
    20581 tccgtaatga atggattata gacggcggct ttcgcttaga agatagcatg gcagcaatga
    20641 atgatttaat gaagctacga gaaagaccaa ctgcgatttt ttctaccgat gcattaaaat
    20701 cattgagtat ttacaaagtg gcagaacaat ctggaaagca gatacctgat gatctttcga
    20761 tcgcaggatt cagtgatccc ttattgtcct ccctattttc gccacagttg actcggatag
    20821 acattccgac aaatcagtta ggaatcaaag ccacagaact tttattccaa ctaatccacc
    20881 accaataccc tgaagaaaaa agaatcattc tttcaaccga gattactctc acagattcga
    20941 tccaacaaca agaaatgtag aacagaatta taaaagaaca gaaacactga ggataagtca
    21001 gttactctgt tcttttttca atcactcatc cctcgttacc ccctcccggt attttagcga
    21061 atttttctta ttttccatct gttttagata gctgtggata actataatgt ttgtgttaaa
    21121 ctattttttc cacagggaag aaaaaactat ttttaaccac gttgtgtata agtttttata
    21181 gttcgttttt ttaaacacat ttacccttat ttcaatccat gttatttggg gatacttatc
    21241 tctaaaacat aaatagaata acagacatgt ctactggttt tgttttattc gaggaattat
    21301 tcaataatag ctagcaaaat attaaaaata accgctctat cttcccatca atttattttg
    21361 cattcaaaaa atgatctatc tttacttttg ttgattgact tctcttggaa gatgatcgtt
    21421 ccgatacctt aattggatac gaaccgatgt tgttaagatc ctttcccact tttcttctag
    21481 ttagacaaat tattttagac aaaccaaaaa agaggttgtg acaataattt gtcacaacct
    21541 cttttttaaa atatctattt tgtttgattg ttcattgcgt cttctgcagc catttgcgct
    21601 tcaatatctg aaatactgta gacattttcg cttgctacac cgacttcttt tggttccatg
    21661 tcacgataac gttgcatacc agtaccagct gggatgatct tacctataat aacattttct
    21721 ttcaaaccaa gaagaggatc tttcttaccg cggatcgcag catccgtcaa gacacgagtt
    21781 gtttcttgga atgatgcagc agataagaaa ctatttgttt ctaatgatgc ttttgtgata
    21841 cctagtaaga caggtcgaga agttgcagga acgcctcctg agactagcgt attgtagttt
    21901 tgatctttaa agtctgcgat gtctaataat gtacctggta agatatctgt atcacctgga
    21961 tccattacac gaactttacg cagcatttga cgaaccatta cttcgatatg cttgtcgccg
    22021 atttctaccc cttgcatacg gtaaacacgt tgtacttcac gaagcaagta gttttcaaca
    22081 gataatacat cgcgaacttg caacaattgt tttggatcga ttgatccttc tgtcaatggt
    22141 gcaccacggt ggatcatatc gccttctgct actttcatac gtgctgtata aggaacagta
    22201 tacgtacgtg tatctgtttt tcctttgatt gttacttctt tggtacgtgt tgcaggatct
    22261 tctgagatat cgatgacatc ccctgttact tctgtgatca ctgcttgccc tttcggatta
    22321 cgtgcttcaa agatttcttg gacacgagga agaccttgag tgatatcgtc tccggcaacc
    22381 ccacctgtat ggaatgtacg catcgtcaac tgagtaccag gttcaccgat tgattgagcg
    22441 gcgatcgtac caactgcttc tccgacttca acgtcagagc cagttgcaag gttgcgtccg
    22501 taacaatgtt tgcagacacc atgttttgta ttacatgtga agacagaacg gattgtcact
    22561 gtttcgacac ctgcatcaac gatttgtttt gcgatgtctt cggaaatcaa ttggtttcct
    22621 gcaatgatca attcatttgt ttcaggatga cgtacttctt tacgggtgta acgtcccact
    22681 aaacgatctt ctaatgattc gatgatttca tttccctcgc ggatggcttg gatttcaaga
    22741 cctcggtcag taccgcaatc ttcttcacgg atgattacat cttgggccac gtcaaccaga
    22801 cgacgagtca agtaacctga atcggcagtc ttcaaggctg tatcggtcat ccctttacga
    22861 gccccgtgag ttgagataaa catttccaag acggatagtc cttcacggaa gttcgaaatg
    22921 attggcaatt ccatgattcg accattagga gcggccatca aaccacgcat tccggcaagc
    22981 tgtgtaaagt tggaaatgtt accacgagct ccagaatcag acatcatgaa gattggattt
    23041 ttcgcttcca aaccttccat cagtttttgt tggatgctgt cttttgcagc attccaaaca
    23101 gcaatgacac gttcataacg ttcgtcatct gtgatcagac cacgacggaa ttgttttgta
    23161 atagtttcaa cttgtttatg ggcattttca atgatttctt gtttttcatt cagtacactg
    23221 atgtcagcga tccctactgt gataccggca tgtgtcgaat gtttgtaccc taagtctttc
    23281 atacggtcaa gcatcttaga agtttctgtt actttgaatc gtttgaagac ttctgcgatg
    23341 atgtttccta agtttttctt cttgaacggt aatactaatt cttgctcttt gatatgtgca
    23401 ggaatatcag taccagcttc aacaaagtat ttgtccggtg tttggacggt caagttgaag
    23461 tctgttggtt cattcagata agggaattca ggaggcatga tctcgttgaa gatgatctta
    23521 ccaactgttg tgatcatcgt acgttctttt tgccattcag taaatggttt ttcacctaat
    23581 gagttagggt tcacaccgat acgtgaatgt aaatgaacat atccgttgcg ccatgcgata
    23641 acggcttcgt tttgatctct gaagaccatt ccttcgcctt cgcgtccttc ttcttccatt
    23701 gtcaagtagt agttacctag aaccatatct tgagaaggag taacaactgg tttaccatct
    23761 ttagggttca agatgttttg agcagctaac atcaacatac gtgcttctgc ttgtgcttct
    23821 tcattcaatg gaacgtggac agccatttgg tctccatcga aatcggcatt ataagcttca
    23881 catacaagtg gatgcaaacg gattgcgcgt ccttgtacta aaactggttc gaatgcttgg
    23941 ataccaagtc ggtgaagcgt aggtgcccgg ttcagcaata ctgggtgttc cttgattacg
    24001 tcttctaaaa catcccatac ttcgtcttct tgacgttcga ttttacgttt cgcatttttg
    24061 atgtttgaag ctagttcgcg ttgaacaagc tcacgcatca cgaatggttt gaataattca
    24121 atggccattt cttttggaag tccacattgg tacatcttca agaaaggtcc aacaacgata
    24181 acggaacgac ctgagtagtc gacacgttta cctagcaagt tttgacggaa acgaccttgt
    24241 ttccctttca acatatgaga aagagatttc aatgggcggt tacctggtcc agtaactgga
    24301 cgtccgcgac gaccgttatc gatcaatgca tctactgctt cttgcagcat acgtttttca
    24361 ttttgaacga tgattccagg tgcattcaag tctaacaaac gtttcaaacg gttattacgg
    24421 ttgattacac gacggtaaag atcattcaag tcacttgttg caaaacgtcc accttctaat
    24481 tgaaccattg ggcgtaaatc tggtggaata acagggataa cgtccataac catccagctt
    24541 ggttgatttc ctgaagcgcg gaatgcttct aaaatatcca aacggcggat tgcacgtgta
    24601 cgtttttgtc cttgtgcaga tttcaattct tctttcaatt cggctgcttc tgcatctaga
    24661 tcaacattat caagtaattg tttaatcgct tctgcaccca tagcagcttg gaattcttgt
    24721 ccatattgtt cacgtttttc gcgatattcg cgttctgtca acaattgttt tttctctaaa
    24781 ttcgtatttc ctggttcgat gactacatat gaagcaaaat aaatgatttc ttccaaagcg
    24841 cgtgggctca tgtctaatac aagacccata cgtgaaggaa tccctttaaa gtaccagata
    24901 tgtgaaacag gagctgctaa ttcaatatgt cccatgcgtt cacggcgaac tttcgaacga
    24961 gtaacttcca ctccgcatcg atcacaaacg attcctttat aacggatacg tttgtatttt
    25021 ccgcaggcac attcccagtc ttttgtagga ccaaaaatcc gctcacagaa caacccttcg
    25081 cgttcaggtt ttaaggtacg atagttaatc gtctcaggtt tttttacttc accgtaagac
    25141 cagcttctga ttttttcggg agaagccaaa cctatttgca tactttcgaa tttatttaca
    25201 tcgatcaaaa ggggttccct ccatttcatt taatggcaaa ccgcaagacg gctgatcttc
    25261 gaccgccttg cagctcatag accggattag tctaaattat cttctgcggt ttcaaagttt
    25321 tggatgacat cgtctttttc atcttccact tgttcagcag ctttcttttc taattctttg
    25381 gcagtttgct gttcggcaaa ttttgtcaaa gcatcaacag tgatcaaatc gtcgtcttca
    25441 tcgtccatgt cgcgaagttc gatctctgtt tcttcgatat ccaagacacg catgtccaga
    25501 cctagtgatt gcaattcttt caccaatacg cggaatgatt ccggtacacc tggttttgga
    25561 attggttcac ccttgacgat tgcttcgtat gttttcacac gtccgactac gtcatctgat
    25621 ttgtatgtca agatttcttg taaagtataa gccgcaccgt aagcttccag tgcccatact
    25681 tccatttcac caaaacgttg tccaccaaat tgagctttac ctcccaatgg ttgttgtgta
    25741 accaatgagt aaggtccgat tgaacgtgca tgcaatttgt catcgaccat gtgagcaagt
    25801 ttgatcatgt acatcacacc gacagacaca cgtccatcaa atggttcacc tgtacgtccg
    25861 tcgtacagaa cagttttcgc atcgctggcc ataccggctt cacgaactgt ttcccatacg
    25921 tcttcatcac ttgcaccatc aaatactggc gttgctacgt ggatacctaa ctgacgtgca
    25981 gccataccca agtgtaattc caatacttgc ccgatgttca tacgtgaagg cacccctaaa
    26041 ggattcagca tgatatcgat tggtgttcca tcaggtagga atggcatatc ttcttccggc
    26101 ataatacggg atacaacccc tttatttccg tgacgaccgg ccattttatc cccttcatgg
    26161 attttacgtt tttgaacgat ataaacacga actaacatgt ttacgcctgg tgacaattca
    26221 tctccagctt cacgagtaaa gattttcaca tcgtgaacga taccgccgcc gccatgtggt
    26281 acacgtaatg atgtatcgcg gacttcacga gctttttcgc caaagatcgc atgcaataga
    26341 cgttcttctg cagataattc tgttacccct tttggcgtga ctttccctac aagcaaatcg
    26401 ccatcttgga cttctgcacc aatacggatg atacccattt cgtctaaatc ttttaatgcg
    26461 tcttccccga cattagggat ttcgcgtgtg atttcttcag gtcctaattt tgtatcgcgg
    26521 gcttctgatt catattcttc aatatggata gaagtgtata cgtcatcttt taccaaacga
    26581 cggctcatga tgatcgcatc ttcatagttg tacccttccc aagtcatgaa accaactaaa
    26641 acgttttggc ctagagccat ttcgccttgt tccatagatg gtccgtctgc cagagtatcc
    26701 cctttttcga ctttctcacc caaatgaacg attggacgtt ggttgtagct tgttccggag
    26761 tttgaacgac ggaattttgt tacatcgtat ttatctaatg caccgttgtc gcggcgtacg
    26821 cggatttcag aagcatcaac gtattcaacg acaccgtcat gtttacataa caaagcagct
    26881 cctgagtcat gcgctgattt gtattccata ccagtaccaa cccatggtgc ttgaggattg
    26941 atcaacggca ctgcttgacg ctgcatgttg gcacccatca aggcacggtt ggagtcatcg
    27001 ttttccaaga aaggaataca tgctgtcgca acggcaacta cttgtttagg tgatacgtcc
    27061 atgtagtcta ctttatcaat agatacttct aagttttcac tttgagcacg agccataaca
    27121 acgtcttgag caaatgttcc atcttcattc aatgggctgt ttgcttgggc tacgatgtaa
    27181 tggtcttcga tatcagctgt caagtagtcg atttggtcag taacacgacc tgtttcacgg
    27241 tctacacgac gataaggtgt ttcgataaat ccgaatttat ttactttcgc ataactggac
    27301 aaactattga tcaacccgat gtttgggcct tcaggagttt cgatcggaca catacggcca
    27361 tagtgggagt agtgaacgtc acgaacttca taaccggcgc ggtcacgagt caatccgcca
    27421 ggtcctaagg ctgaaagacg acgtttgtgt gtcaattcgc caagagggtt tgtttgatcc
    27481 atgaactgtg acaactgaga agaaccaaag aattctttga tacttgccac aactggacgg
    27541 atattgatca attgttgagg tgtcaatgtt tcagtatctt ggatcgacat tctttcacga
    27601 accacacgtt ccatacgtgc tagcccgata cggaattggt tttgtaaaag ttcaccgact
    27661 gaacggatac gacgatttcc taagtggtcg atatcatcga cattgccgat accttccatc
    27721 aagttcaaga aatagctcat tgaagcaatg atgtcggctg gacgaaccgt ttttacagct
    27781 gcttctggat agccgtttcc gatcacgttt acttcacgtt cagggtctct tggtgaaaat
    27841 actttgatga cttggacagt cattggatct gtgaccacac cgtcttctga tggatagtaa
    27901 gtcactgaat ttaggccatt atcaatgaaa ggagctagtg tttccatgac ttggtgagtc
    27961 agaactgttc ctttttcaac gatgatctcg cctgtttctg gatcaactaa tgtttcagct
    28021 aatgtcagat tcaatagacg tgttttcaaa tctaattttt tgtttacttt gtaacgacca
    28081 acgtttgcta agtcgtaacg ttttggatcg aagaaacgag cattcagcaa gttacgtgag
    28141 ctgtctgctg tttttggttc gcctggacgc aaacgttcgt aaacgtcttt caaaccttct
    28201 tctgttcttg agtcgcttgc gtttttatgc aagtcttttt cgactgtatt gcgtaatgtt
    28261 tcgctgtcgc cgaagatttc aaagatcgta tcatcggaac caaatcctaa cgcacgaact
    28321 aatacggtta aaggaatctt acgtgtacgg tcgatacgta cataagagat gtcttttgca
    28381 tcagtttcca tttctagcca agctccacgg ttagggataa cagtagaacc gaatccttct
    28441 tttccgtttt tgtcgacttt tccatggaaa tagacacctg gtgaacggac taactgagaa
    28501 acgattactc gctctgcccc gttgatgatg aatgttcctt gttctgtcat taatgggaaa
    28561 tcaccgaaga atacttcttg tgctttgatt tcgccagtct cgcggtttgt caaacgcaat
    28621 gttacatgta atggtgcaga gtagtttgca tcatgcgcac gagcttctgc tactgtatac
    28681 tttggttctt tcaattcata atctacaaat tccaaagata gattgccatt gaagtcatcg
    28741 attggcaaaa tgtcttcaaa catttctcta agtccctcat ctaagaacca ttggtaagag
    28801 tctgtttgaa tttcgatcaa atttggtaat tccaacactt cactgattct tgcgaaactt
    28861 ctacgttcgc gatgttttcc gtattttact acgtgtccag ccaagctctt cacccctcta
    28921 aaatgtcatt caaaaagact acccgatcaa ttgttacaat aatatttcaa atattatttt
    28981 taggtaacaa cagacatttt ttgcggcttt taggcaaaaa aaaaccaaaa atagaacatt
    29041 ctcttgaaac cttacttcgt gagcttctac ttttgtttcc ttatattaaa gccgaaacgg
    29101 acaatatttc gtttctgcct tttcttcatc agatagtttt tgcaacttca tatactacac
    29161 ttattttcta taaaagtcaa cacttcctgg cgtttcttct gctttttcgc aacattttct
    29221 taatgttttc aattttatgt tttcaattat ctgacaattc ttaattgaca gaccttttta
    29281 tctcggttat actttagaaa attcattttg tggaggaact tctatgcgaa ccaacatcaa
    29341 ccaaatcgca ccaacacaac tgaataactt ttactttagc tactttagat aggtttgtat
    29401 tcatcatgat ggatacaaac gttaaacgac agtttgtatc tatgatggct aaagaacttt
    29461 gtattgcagc aaaaagccac gtagacaaaa tgctaagtgg ctgagcagtg aaaatacaaa
    29521 gtaaaagtat caagcattct actttttcaa tttattttcc gacttttcaa tccatttgga
    29581 cattttgtct gaatggattt tttgtttccc aaaattaagg aggaaaaaga aatgaaaaaa
    29641 aatataatca tcggattagg cgctgccctg ctactagctt taggaggttg cgcacaaaaa
    29701 gacaattccg cagaggcatc agatggacaa aaaacaattg gcgtattgca acttgtacaa
    29761 cataactcgt tagatgctgc ctataaagga ttcaaagaag gattagcaga agaagggtat
    29821 aaagaaggcg ataatctaac aatcgattat caaaatgccc aaaacaatca agacaacctg
    29881 aaaagtatga gtgagaaact cgtcaaagaa gatcctgatt tgctacttgg aatcgccact
    29941 ccagcagcac aatctttatt aaatgaaaca accactatcc cgatgactgt cacagcggtc
    30001 acagacttgg aacaagccca tttagtcaca tccaatgaaa aacctggcgg caatgtgact
    30061 ggcacgacag atatggtgcc aatcgaaaaa caaatcggct tactgttaaa tatcgtgcca
    30121 gacgccaaaa caatcggcat catgtataac aacggtgaag caaactctaa gatccaagct
    30181 gatttggcag aaaaagaatt gaaaaaagcg ggcgtcaaag tcaaagtact gacagcaaat
    30241 tccacaaatg atgtccaaca agtgacgaaa agtctagcta aagatgtaga tggaatctat
    30301 attcctactg ataacacgtt cgcaagtgcg gcaagtgttg tcggtgaagt cgcaaaagaa
    30361 acaaaaacac ctgtcgttgc tggcgcagtt gatcaagtca aagacggcgg tctagcaaca
    30421 gtaggaatcg actacgaaag cttgggcaaa caaacaggga tcatggcagc aaaaatctta
    30481 gacggcaaga atccaagtga acttcctgtc gaaacagcga atgatttgga actgtatgtc
    30541 aataaagaca tggccaaagc attaggcatc aatccagaca gcatcaaaga agctgaataa
    30601 aacacaggag gattctcatg acaaatttgc tctctctttt cttatcatct gcatcgcaag
    30661 gggtcttatg ggccctactt gcgattggtg tatacgtcac tttccgtatc ttggacattg
    30721 ccgacttgac tgctgaagga agctttcctt taggtgcagg gatcgcggca gtaagtatta
    30781 caaatggcta tcatccattg acagcttgtc ttctaggctt tttagggggt gcggcagcag
    30841 ggcttgtttc cggactgctt cacacaaaat tgaaaattcc cgcccttctt gcagggatca
    30901 tcaccatgac tggtttatat tctgtcacat cccgtgtgat gggtgcgccg aacatctctt
    30961 tattgggtca gccaacgatc tttcattggg tcgagtcatt cggcataaca aaagacaacg
    31021 ctgtactgat cgtcggtctg ctttttgctt tgatcgttgt atgtttactt gttttgttct
    31081 tacgtacaga aacaggactt gctattcgtg caactggcga taatctggca atgagcgaag
    31141 caaacgggat caacacagat aatatgaaga tcatcggtta tatgatttct aatggatgca
    31201 ttgctctttc tggctcctta cttgcgcaaa acaacggctt tgctgattta aattctggta
    31261 ttggaacgat cgtaattgga cttgcttcta ttatcatcgc agaagttttg ttacgtaacc
    31321 agccactatt tctccgtctc ttgactgtcg ttttaggtgc ggtcatttat cgttttattt
    31381 tagcgttagt cttcgaactg aatgttgaac caagtgattc gaaacttgct tctgctttcg
    31441 tgctagtgat ctgtctttct ttcccgcaga tccagcaaaa attccattta cctcaaaaaa
    31501 agaaaggcgg tagtgtcaaa tgaatgacgt cttaacattg gaaaatctcc atcatacctt
    31561 tgaaaaagga acgatcaacg aaaatcatgt cctgaaaggc attaatcttt ccttagatcc
    31621 cggcgacttc gtgacgatca tcggtggaaa tggggctggt aaatctactt tgctgaacag
    31681 catcgccggt acattttctg tcgatcaggg tcaaatttta ttgaatggaa aaaatatcac
    31741 gaaaaaatct gttgtcgaac gttcgaaatc catcagtcgc gtattccaag atcctaaact
    31801 agggacagcc gttcgtttaa cggtagaaga aaatcttgct cttgctatga aacgaggaaa
    31861 aaaacgcggc ttcttccgtg gtgtcaaacc acaagaccga acatttttca aagaccagct
    31921 ggttcggcta aatttggggt tggaaaatcg tttatcctca gaaatcggtt tgctgtctgg
    31981 aggacaacgt caagctatta cgttattgat ggccacatta cagcgtccag aactgatctt
    32041 attagatgaa cacactgcag cgcttgaccc tgctacttcc aaaacggtga tggaattgac
    32101 agaaaagctg atcactgagc agaatctcac tgcttttatg gtcactcatg acatggaaga
    32161 cgcgatccgc tatgggagtc gactgatcat gctgcatcaa ggaaggatcg tcgtagatat
    32221 ccagcaagag gaaaaacagc agctcacagt gccccaatta ttagcgttgt tcaaacaaaa
    32281 cagcggcacg gcattaaaag acgatcaagt acttttatct tcataatcag aaagaggatg
    32341 ggacaaaaaa tgaacgccca ccctcttctt ttttctcttt ccttctttcg tttttgcttt
    32401 atgtatcaac cgttgtttct tcgaaaacta ttttgttctc tccttcttcc tgatagaatg
    32461 gatgggaaag gagcttgatg ccatgacaac gaaagacaag gtacttcaat tattgaaaaa
    32521 gacaccagat tttctttccg gagaaaagtt ggcacagcaa ctggaagttt cccggacaag
    32581 tatttggaaa gccatcaaag aattggaaaa ggcaggctat cgttttgagc acgggcctac
    32641 agggtatcgt tacctgccct ctgacgtact agatgcaaaa gaaatccagc aagagctacg
    32701 gtcgtttatt cctgatttga aagtacaaac gatgttgact tctgaatcta cgatgaaaga
    32761 tgccaaactg gcagcagctc aaggagattc tgtttcgaag ttatttgttg ctgatacaca
    32821 agaacaagct cgtggacgtt ttggacggcc ttttttcgct gaacctggac gaggaatcta
    32881 catgagtctc ttgctccatc cagacaaacg gtttgatgaa ttgcctcaat atacgatcat
    32941 cgcagccagt gcctgtgtga aagccattga atctgtaacc ggtcgttctg cttcgatcaa
    33001 atgggtcaat gatatctaca tggatggaaa aaaagtctgt gggattttat ctgaagcagt
    33061 cagtgatatg gaaagcggca ggatcagtca tatcatcatt gggatcgggc ttaatttttc
    33121 tgttcctcaa aatcattttc cagctgatct ccaagaaaaa gccatctctg tctttcccga
    33181 cggacaagct agtgtcacaa ggaatcagct aatcagcaaa atctggcaag aattgttcac
    33241 ttatctttct cacccagaaa aagcgttgga cttctataaa gaaaaatcat tcgtattggg
    33301 aaaaaccgtg tcgttcaatc aaaaaggaac gacttatgtc ggtcttgcaa aagaaatcgg
    33361 tgatcatggc gaactgatcg tccaaacaaa tgaacagaag atgagtttgt cttctggcga
    33421 aatcagtctc acatctatcc gtacgcaata attatcagat ttcacaaaag gacacctgat
    33481 ctcatacagt gtcctttttt ctgaattctc ttcaaaagac tctccttatt ctatcagctg
    33541 acagtttctc tatcctctag aaaaaggtat tctaaaaata tctataaagc atggtacact
    33601 ttctttaaat gaatcaatgc aaaaaacaag agagtcatca gtttacacga tcaaaagaga
    33661 tagcaggccg cttccttttt caatcttata gatagaccat gggtaacgat aaaaacaaaa
    33721 aagaacttgc gacttatttt gccgtaagtt cttttttagc tagtgcctta atctcttaat
    33781 cccaacaaac gtctcgcata gatcacctga ccagagtgca tagcagcatc atcaataata
    33841 gaaacgagac gcattcctcg tgtgactggt ggtgtccagt tctcgtcaat tacttcatct
    33901 agctcggaag gttccagcct tcttaagtaa gaaatagcaa aatcagcagc tcctcgcaag
    33961 tatcctagca gatcttctat ttgattagct tggatctgct ttgcttgttc cagactatgc
    34021 ttccaatctt gttcgttatc tgctatcgtc atcggaaacc ggctttccca gtcttgacta
    34081 tgccaaattg gagaagttcc tgctaaatca gcaatctgga aatctaattc tcgagcggta
    34141 tgccaagcca gccaagtcat tgatttgatc tgcggggctc tatcttccac tggaaaacga
    34201 ttggcctcct caatagagac gccttcaaac aaacgttcaa accgatcgta tgcccgatcc
    34261 acactctcgc atagcatatc taaataatcc atttattttc ctccttttta tttatcctac
    34321 ttactttttc ctaattctag gatacctact tatatgttac tacacaccct tattttttac
    34381 actttaacag tcaccatcat caatagtttg gagattgaaa gaaaaagata gtgtcgaact
    34441 atcaattgaa caagaagcaa caatatgaac gtttcattaa tattctttaa ataaacaatg
    34501 atttttcaaa cgaacagttt aagactagga gaaaaagagt ataataggaa aatgtttgaa
    34561 aggaaaggaa ggggaaaaat gaaaaaaagg gcaagtttac tagtcctagt tgctttgttg
    34621 ttcaatatct tttcgtatgg tgtggccgtt tcagcggaaa catacattcc taaagaccct
    34681 gggaatattt ataatgatct aactggtaac tatggcttgt taggaattgc tagtcaattc
    34741 cacgtatttg ctaaaaataa gacaactgtg aatgcacata cagacggaaa tatagcaaca
    34801 catgaacttg atgcaaaaaa taattttggt acagagcttc atgaaggttt attgaaccaa
    34861 gaaataaatt atatacaaaa aactgattct ttcgctaatt cttcaggcat acctacaggt
    34921 agcacaagaa tgaataaatt tgtcgttggc agtaacgtgg atgtagcact tcaagatggt
    34981 catcctacag tcaatggatc caggcttgat cacttaactc cagatgattt ttataaagat
    35041 cgtgctgggc atgaatatat tgatttcgag caggaatttg ctaaattgaa tgtagcagca
    35101 aatgatcttg caacgatcac tccagctaaa acatatactg ctgcagattt tccagacatg
    35161 aacaatcgga caatcgattt aactgattta tcagataccg ggtttttatt ggtaaatatc
    35221 gatgcagaag tgttaacgat gaatacgcca cttcaaatca ttaatccaaa tgatcaagta
    35281 gttgtattta atgtcatcaa cagttcttct gcattgaacg tacagtcacc aatcaaatac
    35341 aatgatcgtt caaatcatga aacagaagat ttttcggatg cgaatatctc ttggaatttt
    35401 ggaaatgaga tgactgattt gacaatcagt gctccttttc aaggaacaat ccttgcgcct
    35461 aatgctacta tccgcattac tcaaaaccaa gacggaacga ttataggaac aaatgtgatt
    35521 ttagatgcag ctaccaatcg ttgggatcct aacgaaatat ttatcagcga caatggtaca
    35581 gatacgacag acacaagcga cagtacagat acatctacca caagtgatag ctcagattca
    35641 tca
//
LOCUS       DACEZN010000012        34708 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEZN010000012 DACEZN010000000
VERSION     DACEZN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.012"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..411
                     /locus_tag="IXH60_000431"
     CDS             <1..411
                     /locus_tag="IXH60_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1989399.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="IXH60_000432"
     CDS             509..697
                     /locus_tag="IXH60_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1989400.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="IXH60_000433"
     CDS             complement(755..1108)
                     /locus_tag="IXH60_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1989401.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="IXH60_000434"
     CDS             1639..2061
                     /locus_tag="IXH60_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1989402.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="IXH60_000435"
     CDS             2058..2288
                     /locus_tag="IXH60_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1989403.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="IXH60_000436"
     CDS             2786..2986
                     /locus_tag="IXH60_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1989404.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="IXH60_000437"
     CDS             3013..4206
                     /locus_tag="IXH60_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1989405.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IXH60_000438"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IXH60_000438"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1989406.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="IXH60_000439"
     CDS             complement(5898..6821)
                     /locus_tag="IXH60_000439"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1989407.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="IXH60_000440"
     CDS             7017..7622
                     /locus_tag="IXH60_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1989408.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="IXH60_000441"
     CDS             7930..8946
                     /locus_tag="IXH60_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1989409.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="IXH60_000442"
     CDS             9087..10388
                     /locus_tag="IXH60_000442"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1989410.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="IXH60_000443"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="IXH60_000443"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1989411.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="IXH60_000444"
     CDS             11117..11515
                     /locus_tag="IXH60_000444"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1989412.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="IXH60_000445"
     CDS             11581..12663
                     /locus_tag="IXH60_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1989413.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="IXH60_000446"
     CDS             12875..14437
                     /locus_tag="IXH60_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989414.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="IXH60_000447"
     CDS             14430..15563
                     /locus_tag="IXH60_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989415.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="IXH60_000448"
     CDS             15563..16519
                     /locus_tag="IXH60_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989416.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="IXH60_000449"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="IXH60_000449"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1989417.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="IXH60_000450"
     CDS             17808..18626
                     /locus_tag="IXH60_000450"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1989418.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="IXH60_000451"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="IXH60_000451"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1989419.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IXH60_000452"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IXH60_000452"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1989420.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="IXH60_000453"
     CDS             complement(20506..21087)
                     /locus_tag="IXH60_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1989421.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IXH60_000454"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IXH60_000454"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1989422.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="IXH60_000455"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="IXH60_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1989423.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="IXH60_000456"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="IXH60_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1989424.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="IXH60_000457"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="IXH60_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1989425.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="IXH60_000458"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="IXH60_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1989426.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="IXH60_000459"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="IXH60_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1989427.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="IXH60_000460"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="IXH60_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1989428.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="IXH60_000461"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="IXH60_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1989429.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="IXH60_000462"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="IXH60_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1989430.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="IXH60_000463"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="IXH60_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1989431.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..30238
                     /gene="rpsS"
                     /locus_tag="IXH60_000464"
     CDS             29960..30238
                     /gene="rpsS"
                     /locus_tag="IXH60_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1989432.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30261..30608
                     /gene="rplV"
                     /locus_tag="IXH60_000465"
     CDS             30261..30608
                     /gene="rplV"
                     /locus_tag="IXH60_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1989433.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30622..31278
                     /gene="rpsC"
                     /locus_tag="IXH60_000466"
     CDS             30622..31278
                     /gene="rpsC"
                     /locus_tag="IXH60_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1989434.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31281..31715
                     /gene="rplP"
                     /locus_tag="IXH60_000467"
     CDS             31281..31715
                     /gene="rplP"
                     /locus_tag="IXH60_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1989435.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31705..31893
                     /gene="rpmC"
                     /locus_tag="IXH60_000468"
     CDS             31705..31893
                     /gene="rpmC"
                     /locus_tag="IXH60_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1989436.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31918..32184
                     /gene="rpsQ"
                     /locus_tag="IXH60_000469"
     CDS             31918..32184
                     /gene="rpsQ"
                     /locus_tag="IXH60_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1989437.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32242..32610
                     /gene="rplN"
                     /locus_tag="IXH60_000470"
     CDS             32242..32610
                     /gene="rplN"
                     /locus_tag="IXH60_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1989438.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32647..32955
                     /gene="rplX"
                     /locus_tag="IXH60_000471"
     CDS             32647..32955
                     /gene="rplX"
                     /locus_tag="IXH60_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1989439.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32983..33522
                     /gene="rplE"
                     /locus_tag="IXH60_000472"
     CDS             32983..33522
                     /gene="rplE"
                     /locus_tag="IXH60_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1989440.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33541..33726
                     /locus_tag="IXH60_000473"
     CDS             33541..33726
                     /locus_tag="IXH60_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1989441.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33762..34160
                     /gene="rpsH"
                     /locus_tag="IXH60_000474"
     CDS             33762..34160
                     /gene="rpsH"
                     /locus_tag="IXH60_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1989442.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34192..>34708
                     /gene="rplF"
                     /locus_tag="IXH60_000475"
     CDS             34192..>34708
                     /gene="rplF"
                     /locus_tag="IXH60_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1989443.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRK"
BASE COUNT        11214 a         6395 c         7384 g         9715 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttctat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30181 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30241 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30301 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30361 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30421 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30481 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30541 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    30601 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    30661 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    30721 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    30781 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    30841 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    30901 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    30961 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31021 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31081 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31141 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31201 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31261 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31321 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31381 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31441 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31501 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31561 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    31621 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    31681 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    31741 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    31801 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    31861 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    31921 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    31981 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32041 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32101 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32161 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32221 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32281 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32341 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32401 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32461 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32521 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    32581 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    32641 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    32701 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    32761 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    32821 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    32881 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    32941 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33001 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33061 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33121 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33181 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33241 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33301 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33361 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33421 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33481 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33541 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    33601 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    33661 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    33721 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    33781 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    33841 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    33901 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    33961 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34021 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34081 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34141 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34201 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34261 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34321 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34381 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34441 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34501 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34561 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    34621 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    34681 tatgttggcg aatatgtacg ccgtaaag
//
LOCUS       DACEZN010000013        34250 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEZN010000013 DACEZN010000000
VERSION     DACEZN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.013"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(60..404)
                     /locus_tag="IXH60_000476"
     CDS             complement(60..404)
                     /locus_tag="IXH60_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1989444.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(541..1677)
                     /locus_tag="IXH60_000477"
     CDS             complement(541..1677)
                     /locus_tag="IXH60_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1989445.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(1866..2840)
                     /locus_tag="IXH60_000478"
     CDS             complement(1866..2840)
                     /locus_tag="IXH60_000478"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1989446.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(2920..3795)
                     /locus_tag="IXH60_000479"
     CDS             complement(2920..3795)
                     /locus_tag="IXH60_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1989447.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3925..5832
                     /locus_tag="IXH60_000480"
     CDS             3925..5832
                     /locus_tag="IXH60_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1989448.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(6373..9651)
                     /locus_tag="IXH60_000481"
     CDS             complement(6373..9651)
                     /locus_tag="IXH60_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1989449.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(9922..10467)
                     /locus_tag="IXH60_000482"
     CDS             complement(9922..10467)
                     /locus_tag="IXH60_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1989450.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(10479..11171)
                     /locus_tag="IXH60_000483"
     CDS             complement(10479..11171)
                     /locus_tag="IXH60_000483"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1989451.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(11190..11474)
                     /locus_tag="IXH60_000484"
     CDS             complement(11190..11474)
                     /locus_tag="IXH60_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989452.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(11487..12050)
                     /locus_tag="IXH60_000485"
     CDS             complement(11487..12050)
                     /locus_tag="IXH60_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1989453.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            12278..12940
                     /locus_tag="IXH60_000486"
     CDS             12278..12940
                     /locus_tag="IXH60_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1989454.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            13064..14260
                     /locus_tag="IXH60_000487"
     CDS             13064..14260
                     /locus_tag="IXH60_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1989455.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(14361..15737)
                     /locus_tag="IXH60_000488"
     CDS             complement(14361..15737)
                     /locus_tag="IXH60_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1989456.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(15774..16640)
                     /locus_tag="IXH60_000489"
     CDS             complement(15774..16640)
                     /locus_tag="IXH60_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1989457.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(16729..17754)
                     /locus_tag="IXH60_000490"
     CDS             complement(16729..17754)
                     /locus_tag="IXH60_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1989458.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(17772..18446)
                     /locus_tag="IXH60_000491"
     CDS             complement(17772..18446)
                     /locus_tag="IXH60_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1989459.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDNNLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(18630..19523)
                     /locus_tag="IXH60_000492"
     CDS             complement(18630..19523)
                     /locus_tag="IXH60_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1989460.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(19597..21294)
                     /locus_tag="IXH60_000493"
     CDS             complement(19597..21294)
                     /locus_tag="IXH60_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1989461.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(21287..22600)
                     /locus_tag="IXH60_000494"
     CDS             complement(21287..22600)
                     /locus_tag="IXH60_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1989462.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(22600..26637)
                     /locus_tag="IXH60_000495"
     CDS             complement(22600..26637)
                     /locus_tag="IXH60_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1989463.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(26650..27108)
                     /locus_tag="IXH60_000496"
     CDS             complement(26650..27108)
                     /locus_tag="IXH60_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1989464.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(27406..27924)
                     /locus_tag="IXH60_000497"
     CDS             complement(27406..27924)
                     /locus_tag="IXH60_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1989465.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(27926..28543)
                     /locus_tag="IXH60_000498"
     CDS             complement(27926..28543)
                     /locus_tag="IXH60_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1989466.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(28540..29403)
                     /locus_tag="IXH60_000499"
     CDS             complement(28540..29403)
                     /locus_tag="IXH60_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1989467.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(29390..30292)
                     /locus_tag="IXH60_000500"
     CDS             complement(29390..30292)
                     /locus_tag="IXH60_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1989468.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAGYAMAMSMILFLI
                     VLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(30333..31667)
                     /locus_tag="IXH60_000501"
     CDS             complement(30333..31667)
                     /locus_tag="IXH60_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1989469.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            31875..32705
                     /locus_tag="IXH60_000502"
     CDS             31875..32705
                     /locus_tag="IXH60_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1989470.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(32949..33198)
                     /locus_tag="IXH60_000503"
                     /pseudo
     CDS             complement(32949..33198)
                     /locus_tag="IXH60_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33389..33583)
                     /locus_tag="IXH60_000504"
     CDS             complement(33389..33583)
                     /locus_tag="IXH60_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989471.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         9945 a         7361 c         6035 g        10909 t
ORIGIN      
        1 aaagttagtc atctaacaat aggccgtttc aaaactagtt gtctttttat atccacagat
       61 cattcgactt tttcgaataa cccacggcta atcatcccat ccatcagaaa ataaaattta
      121 tcttggctca ttttctgttt tggatctttg aaaatattga ctgctttcaa tccatttgct
      181 gtagtgatcg acattttcaa tgtttgaata tcttttgata cagtgatttt taatttgaat
      241 ccttctttgc tttgcggcgt ggcatctaat ggtcggatca attgataatt gcctccgttt
      301 gtttctgtaa atccattatc gcgcaatgta tatctttttg catcaggatg caaacgatag
      361 ctaattggtg cgcccagtac cgttgctgtt gtttcaaatg ccatgatttt cacctcgata
      421 aaattagttt ctctttatta taaaagttta tcctgatttt tactaggttt ttcagttaag
      481 ttccctaaaa aatgaaggcg ttctctcaga catctgttca tataagaatg acttcactag
      541 ttattttttg acagcacgaa ctaattgatc aaggaaatac tggatgtcgt cttcggtatt
      601 tcccaaacca aagctgatac ggatggattc ttttagtatc ggtgtatctt ctccatacat
      661 tgcttctaat acgtgtgaag gctcgatatt tcctgctgta caagctgaac cagtagaaat
      721 agcgacccca ttaagatcaa gcttcatcag tagcaaatca tttgcttcgc caattaagcg
      781 aagattcaac acatgaggga gtttattcgt taaatcacca tttacttgat aagggatttc
      841 gtttgcttct aatgtatcaa gaatcaattg ctggaatcct ttatatttgt tgttacgtgc
      901 ttcacgttct tcaggtgaca gcagttcaac tgcttttgcc ataccccaga tccctgctaa
      961 attttctgta cctgcacgcc gcttttcttc ctgctccccg cctaaaagca atgcagggat
     1021 atttactccg tctcgtttat ataagaaacc gacgcctttt ggcccattga ttttatgagc
     1081 agagatactt aataaatcga ttcctaattc ttctggatgg atcgtttggt ttccgtacgc
     1141 ttgtaccgca tctgtatgga aataagctgg atgatccgct aaaagttcac cgatttcttt
     1201 gattggcatc aaatttccga tttcattgtt cccgtacatc acagagacca agatcgtatc
     1261 ttctcgtaac gcttttttga cctcttcgat cgacagattc cctttttcat ctacaggcag
     1321 atatgtaacg tcaaacccct ttgtctctaa atatttcatg gtattaagaa cagcaggatg
     1381 ttcgatagct gtagtgatta gatgtctgcc ttctttttga cgcgagaacg ctgtttctaa
     1441 gatagctgta ttatcccctt ctgttccccc actgttaaag atgatctcgt gaggttttgc
     1501 ttgcagactg tctgcaatga tttgtcttgc tgtttccaat ttttcatgcg cgtggcgacc
     1561 aaatccatgt atgcttgaag gatttccaaa cgtgtcttgc atgattgccg tcatcgcttc
     1621 aattacttgt ggatgcatgg gggtagttgc cgcatgatcc agataaatag ttttcacttc
     1681 attcatccct tccaaattat tgctaggcac attctagcat aaaatagcga acaaaccaaa
     1741 gaagagctca tttttgtcta gaggacgttt catgggcata gaaaaaagga gctgtgacaa
     1801 aagtcttgtc gcagctcctt gaagctgctc acttctgtca caacttcttt ccctcttttt
     1861 tttgtttatt cgaattcttt ttcttccaga cggaataacg gactcatcgg tgtattttca
     1921 tggatgcgtt tgaccgcttc acccattaat tctgaacatg taacgattgt taagttttct
     1981 ggatgacgat tttcttctgt gaaaacagaa tcagtgatac aaatatcttt gattggtgca
     2041 ttgtctaaaa tacttttcgc atgatcggac aataatccat gtgacgcaca ggcatatact
     2101 tcttttgcgc cgttttcttt taatacattt gctgctcgtg caagcgttgc tcctgtgttc
     2161 aagatatcat ctaccatgat acatgtcttt ccttgaacat caccgatcac ataaccaccc
     2221 tcagttgaag catcatctgc atgatctacg atggctaatg tgctgttcaa atattctgaa
     2281 agactacgtg cacgctgaac accgctgttt tttggcgaaa caactacgat gtcatctccc
     2341 gtcagaccta attgacggta ataatgagca aacaacggca tcgtaaataa attatctaca
     2401 ggaatatcaa agaatccttg tacttgaacc gtgtgcagat ctaaagtcag tactcgagtt
     2461 gcccctgctt cttcgatcaa gttagcgatc aatttcgcag tgatcggttc atgaggtttc
     2521 gccgtgcggt cttgcctagc atacccatag taaggcaata cgacattgat cgttttcgca
     2581 cttgctcgct tcattgcatc gatcatgatc agtagttcca tatagtaatc attgacaggt
     2641 gcattcgttg attgaacgat atacacatga tcccctcgaa tactttcttc gatattgatg
     2701 gaaatctcgc catcactaaa ttgcttcacg acgcttttac ctaattctgt tccgaacact
     2761 ttcgcgattt tttcagctaa tggacggttg ccattcaaac taaaaatctt caacgtgtca
     2821 tcctgatact tattactcat gtagtcctcc ccaaaacttc tctattcttc gatagtttaa
     2881 catatttttc caagattttc tgttaatttc agcaaattct taaggaagtg aaactgttcc
     2941 gttactccaa ctggcaggaa tgactttatg ataatcgccc cagttgtcgt gtactttgta
     3001 ataacgttct cctgtttcag tttcaaaata agcgtacgct gtcacccaat gattgccata
     3061 tgtactgcca agtactttca aaataccaat catcacaggt ttgccttcac gtatacgttt
     3121 tgtagctcgc tgccatgaac ctagaagagt tgctcgtgca cgctcatgat tgccattctt
     3181 ctttaaaaaa ctagaaatcc ctgtactaac ctgaaaagga atcgtaggaa gtcctagtgg
     3241 ttggatctgg ctacgcaatg ccttcgtcaa aaccaacgaa tcagccgtat ttttcttgcg
     3301 aatctcattt ggaagcatcc accccttacg atagtcttga taataggcaa ggagaactgc
     3361 tgccgcgtaa gtaccacaaa gatagcctga gggggtagcc catttcttat acctcaaaaa
     3421 acgttcttct ggcaatccca cccagcaatt tgcgagttcc tttcctgacc ttttttcttc
     3481 gatcaaaacg ggaatatctt cttttctgtt gatccatcta gataactcga ttggctcttc
     3541 attgatcagc ttccattttt tacaagaaag accctcttga taagtatatt ttgctggaga
     3601 atattctacc ggatgtcctg attgatcgat aatcacataa ctttgatctt caaaaaccag
     3661 agctaaaaaa agcaatttac tttttcttga ctgcagctgc tgagtgctgg ctaatttttt
     3721 aattttttta tctactttga aatataaagg tttatccact acttgattca gttgggatcg
     3781 cctccttttt ttcattataa gtgaaggatt gaaaaaaaca aatcagtttg attgttcgaa
     3841 ttttcaattt tttatgttct atttgttaat tccttcattt tgtaagataa tgggcacacg
     3901 aaacaaaaaa ggagcgatcg atgaatgaac aaagaacgat tgaaacaaga tttctatgaa
     3961 gcagtcaatg aagaatggca aaaaacagca aaaatccctg cagataaacc agctacaggc
     4021 ggtttccaag atttagttga cgggatcgac caacttttga tgggagaaac agacgatctg
     4081 cttgcccatc cagaaaaagt gtcttcagaa aaaatgaaac atttccttgc atattatcaa
     4141 ctggcaaacg attatgaccg aagaaatgag ttgcatgctc aaccgcttca acctataatc
     4201 gaaaaagttg aatcattgca gtctttcgac gaattgaata agcaatttcc agaatggctg
     4261 ttggatacat taccattgcc atttaatctg gatgtagatg cagatatgaa gaatgcgcaa
     4321 acgaacgcct tctttgctta tccgccttct ttattccttc cagataagac gtattatgca
     4381 tcagaccatc ccaacggtgc acaactaata ggcgttttct ttgatatggc tgttcagtta
     4441 ttagagcttg ccggcaaaga aaaaagtcag gcagaagcaa tcacggaaca agcagtgcaa
     4501 tttgataaat tgattgctcc tcatgtaaaa agtgcagaag aaaacgcaga ttacagcaaa
     4561 atgtataatc cgcaatcttt tgatacattt attaaataca cagataaact ggacttgaaa
     4621 aaacttgtca cgggtttgat tgggacaact ccagataaag tgatcgtaac tgatccagtg
     4681 tacttcgatc atttgaatga catcctgaca gaagctcatt ttccgttatt caaaagctgg
     4741 atgatcatta aaacagtacg ttctcttagt ggttatctat cagaagaatt ccgccaagtc
     4801 agcggaatct attctcgcgt attgtctgga acagacgaag caatgcctca gaaaaaagca
     4861 gcgtattacc ttgcctctgg acaattcgat caagtagttg gcgattacta cggaaaaaaa
     4921 tattttggcg aagctgctaa aaaagacgta gaacaaatgg tgaaaaaaat gatcgccgtt
     4981 tataaaaaac gtctagaaga aaatgactgg cttggggatg caacaaaaga aaaagccgtc
     5041 ataaaattaa ataacttagg tatccaagta ggctaccccg ataagatccc agcattattc
     5101 gaccagttca aaacagtacc tgcagatcag ggtggtactt tgctttcaaa tactcttgtt
     5161 ttcagcaaac tcacattaaa agatcgtttc agcaaatgga acaaaccagt ggatcgttca
     5221 gaatgggaaa tgagtgctga cacggtcaat gcttattatc atccatttcg gaatgtgatc
     5281 gtctttccgg cagcgatcct tcaagcacca ttttacagtc tagaacaatc aagctctgct
     5341 aattatggtg gaatcggagc tgttatcgct catgagattt cccatgcatt tgataataac
     5401 ggcgctttat ttgacgaata tggcaacttg aacaactggt ggacagaaga agatcttaca
     5461 caatttcaaa acaaagcaca agcaatgatc gaagaattcg atggtctgcc ttttgctgac
     5521 ggaaaagtaa acggaaaatt aaccgtttcg gaaaatattg ctgatgcagg cggtctaagc
     5581 tgtgcattgg aagcagcaaa aacggaatca gatctttcta tcgaggaatt ctttgtcaat
     5641 tgggcaacga tttggcgtac aaaagcaaaa aaagaatacc aacaactttt actgcaaatc
     5701 gatgtccatg cccctgctaa actccgagca aacgtgcaac caaaaaacct tgacgaattc
     5761 tatgaaacat ttggtatcac ggaacaagac gctatgtatc tagcgccaga aaaaagagtg
     5821 cgaatctggt aagaggttgt gccagaagtg gtcagcttca agaaataaga aggaatttgg
     5881 gaaaatcgct tctcaaattt ttgtgacatt tcggcttatt tttcgaagaa gttacttctg
     5941 gaataccatg tattagaggt tgtgaaaaag ctgcttagtt tcaagtagta agaaaaattt
     6001 tgtgaaaatg gctttccaca tttttgcata aatttgactt actaccgaga aactggcttt
     6061 tgaacaccgt ttatcgagag gttgtgaaat cagcgtcctg acctgagtat tagagaaaaa
     6121 aacggaaaaa tagttcctca tattttgccg tttgttgagc taataccgag ggtcagctga
     6181 ttgaacaccg tttagtggag agattgtgaa aaagctgtcc tgcatcaagt aataagaaca
     6241 gccaatcaaa agcagcaagg agaacctcat ctataaaaag atagaaggaa gtgagacatc
     6301 tattggtctc atttccttct atttttactt tcctcagtca attgttttac atcataaaag
     6361 gagaaattcc acttattttt ttcgctgttt catgacataa acaactacac cgattaacaa
     6421 aattcctcca gctaatgcta gcaacgggtt attaaattct cccgtttttg ggagtccttg
     6481 agtgccattt ttttcacttt cttttgctgt cctatccgga tgagaaggtt ttccatttgg
     6541 ttttccattt ggtgattcat ttcctggatt ttctctttct ggtttgtcat ttcccggggg
     6601 cacatcggca gtttctcgca cagtgatcgc tttttgctgg gtaacagatt ctgtatgagc
     6661 agaccatttt tccccatcaa atatttccga acgataagtg acttctagcg tatattctcc
     6721 ttttgaaggt gctgaaaaaa tagcggtatg attagaaaat tgagcacttg actgtgtttc
     6781 aacgatttgg atcttttctg ggaaataacg catatcttca aagacaggcg ttccttgatt
     6841 tggtgtgaaa gttgacacaa aaactgtaaa aggcttcagt tcaatgatat ttccttctgc
     6901 attctccgcc acaccatttg cttcttgtat aataatcgga ttattttgtt catttggttt
     6961 tgtctgattt ggttcttgta cgattgcata ctctggatat tccgaagacg gtgaggtttc
     7021 tccgtcaaaa atagcaccta cagataatgg aagaccatcc actctttcct tttctgtttg
     7081 acgattcccc ggtttaatct gcatcagtcc atgaacatgg atacttccat tttcatagcg
     7141 cgtaacaggc tgctgacctt ctttcgaaca tgtatttaga gactcgatcc aatcttttga
     7201 tatggtatgt ccaagagtat tttctgattg tttctctcgt tcattccaat agtagttcaa
     7261 cgagtggtag atttgatctt cgttttgatt caatgggcgg atctgctctc tcatctcaag
     7321 attctctgtt cggaaagata agataccaga agcatggtag tctgtgtttc ctgctgaaga
     7381 ggtataaaga gccacattat atgaaccgtt attaaaactt gtactgctat tgacgataat
     7441 atctgttccg ctattggaat cgattccttt cgcaagattt tcgaaagaca aactattgat
     7501 caattcatga gggcccgtta aagaagaacc gcccattttg aatccattac cgttaccttc
     7561 tccctcaatt cctggtaccc agccgtttcg ataagcaact gagtttttga tgattacttt
     7621 tccgatcgaa cctgtttcat ttttagcaaa aaggtcccaa ccgtcatctg cattatgata
     7681 cgcgatacaa ccgtcaaata catttccttc tcctattgtc agtttggccg caaatccatc
     7741 ggcatcttca aatcctggat cggcattttc aaaagatgta cagttcttga ctaagttatg
     7801 cgcgggccat gcttcgaaag gatcatttga gctaccgctg atttggattc ctgtatttcc
     7861 attccgataa gcatgaatct cttcgatcaa attatgattt ccagaaatct gtaatccttt
     7921 ttgcatagct gcactatttg taatgtcaaa ccctcgtaaa ccccagtagt gactgaacag
     7981 tgtcatccca tttccagttc ctttaaaatc aagtacagga cgcttgtttt ctggattttt
     8041 ctctgcaatc aactgaatcg ggttttctct tgtcccgtta acgccttttg gaatcgtcaa
     8101 tcctctttcg aaggaataag ttccaggcgc catgacgatt gtctgcccag catatgcgta
     8161 tctcaatgct tgtgtcagag ataatggatt ttgtctgctt ccgtttcctg catctgaccc
     8221 ttctggtgtt acataaactg tcgaatattt aggtttacgg tagtccactg tatgaagaat
     8281 ctcttttgtt tcataactag tcatctctac gtattcttct ggcggatagt ttttatctgg
     8341 cgtaaaactg aaagtaaatt cgttcgttcc ttccatcagt tttgtctgat aatcaaattc
     8401 ccgattcgca gtgatagcca agttttttgc gttcttaagc acctctcctg ttgctttatt
     8461 tttaattgtc aataatccgt cagcatttgc tctaaaggac atagtatgat cttggtttcc
     8521 tgaatgatta ctagaggtca cagtgtaaat cggatcaatc aagcgagtag gtctttcttg
     8581 cccaggagga tctgtcgcag gatctgagta agcaacttcg attccttcta ccttgatcgt
     8641 catattcctt gctgcagcaa atcctgcata aataaccgat tcatctgaag cgtataattt
     8701 ttcccagtca tacatgatgt cagaagccat ttcttctcct gtttcaggat cgtaataata
     8761 tgcctctaaa ccagtattcg tttttttcat ctctacgtcc agtttagtaa tcgagttcgt
     8821 ggccggaaac agattcccat gaccgtttcc gaatatatta taagaacctg tttctttttc
     8881 caagaaccct gctgaagtct ctaaaggtgt tgtttccact gagacactcc caggagctgg
     8941 cgcagcttgc ggatcttcag atgatattcc tgtgattgcc cgtgtcccca atccaagtcg
     9001 catcgtatat ttgctgccgc tcgtgttgac cacttcttgt ctatccgagt cccagacata
     9061 ttcgatccga ctgcctagta tggcaaatga atttgaataa aatgcctgcc caaacaaatg
     9121 attttttggt actgcgtctc ttaccattaa agcaaatcct tcttgggcat tcgtataggt
     9181 ccaagattct atttcgatcg tcgcccgcat cctaaagttg atgtctttgg ggattgcttg
     9241 ataataatag gtcattccat ctgatccgct tgactggaat tttccgccgt tattcgtgga
     9301 agagagccgc acgccgtctt ctaatggttc aatggcatac ctactagcac ttgtgctttc
     9361 tccgaaaaca acccctttcc actcttcatt tgcttcttca gattgctttt ttgtcttttc
     9421 attttctgat tctttcatca taggatcatt tccttcttcc gtatccatat cttcactagt
     9481 tcttggtatt tcctccattg ttgacatccc ttcaatgttc acgtgtgcat tttcttcgtt
     9541 cacagactgt tgttcgttag cagcaacttc aacgacagaa attaaaaccg gggtactgag
     9601 cataaggaga ttcatcatta gcgcactgta ttttatacgt ttttttaaca agtgtattcc
     9661 tcccgtaaca aattagaaag cgtttactta aagtattacc taatttattt ttactgtcaa
     9721 taaaaaacaa aattataata agtgtatttt ataaaaagaa aaaatataac ttaagttgtt
     9781 gataataaag agatttatta acggaaaaag aataagtttt tttaaaataa cgtataaaaa
     9841 agagcataaa acaaagtcgt ttgttttatg ctcttctttt attattttgt gctttttatt
     9901 ttatcaaatc actttgtttt tttataaaat ttctgcgcct agtgtggact caaaatgtct
     9961 caatgcccat tcatgtccaa cgggatcgaa acttgccacg gaatctttga aaatatggag
    10021 tttgtatcct agattatacg catcgactgc tgtatgcaac acacagatat ctgtacatac
    10081 gccggtcaaa taaatatctg tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc
    10141 actaaaagcg gaataatgcc gtttgtctat ccaataaaca ttttcctctt gctcatagtc
    10201 ttggtataaa ctttttaaag agccgtacaa ttcccgtcca cccgttccta tcacattatg
    10261 cggaggaaat aagcggttct ccggatgaaa ggaatctttt ggatcatgcg catcaatggc
    10321 aaaaacgaca aaatcctttt gatcaataaa cttctttgta tgagcagtca gctttttttc
    10381 aattacttgt cctgctgtcc cagtagtcag ctttccatcc tctgcaacaa aatcatatgt
    10441 gtagtcaatc gagatcaatg cgttcatctt agtcctcctt atacgagatg tttgacaaat
    10501 tcgatcacca tttctgctga tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt
    10561 gtcgccgagt gatctgctgt gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac
    10621 tgacgagctg tttgtccaac agcggctcct tccatttcgc aagctaatgc ttcagggaaa
    10681 ttcgttaaga tttctttgat tttatctggc gagtcgacaa atgtatcacc tgtaacgatc
    10741 aatccttctt ttgcgttcaa attcgttgct tttgctgctt taaccatttc actgcgcaaa
    10801 tatgtgcttg cttcataata tagaggcatt cctggtaatt gtcctgggtt gtagccaaat
    10861 cctgttgcat ctgcatcaaa ataagctact ttgtctgata taacgatgtc acctacttgt
    10921 aagccttcac cgattccgcc agctgaacct gtattgatca ccatgttcac gccgtattgt
    10981 tggatcaaca gacttgtggt aatagatgct aagacttttc ctataccgga acgtacgacg
    11041 atcacttcgt ggttacctaa tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat
    11101 aatggttcgc ttaggttctc tctcaaaatt tttacttctt cttccattgc gccgataata
    11161 ccgattttca tctttttaat tccttctttc tatataaatg cgattgctaa aaaaaccaca
    11221 cataacatta ggataacaat aataagagat ttcatcaaaa aagagttcca tttggtcgtc
    11281 ttttcccgtt cgctgatcct tgttttcgtg attggtttat tctttttgct ttctttacga
    11341 taaaagctgg caatcttttt ttcttcttgc tgataagctg tttctgcttt tcgctgtttt
    11401 tttaatgact cgcttgcttg tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc
    11461 ggtcctttgg ccataagccg atccctctac tttcctttgg tcaatagttc ccagtattga
    11521 atcgcataaa tcgtctttgc atcacaaatc agctgatctt tcatcgcttg ttgcgcctct
    11581 tcaagagtta gatgatacaa ctccagtact tcgtcttcat cttgtgctaa aggattctcg
    11641 accttctcta ccccttgtgc atgataaata tgaaggactt catttgcaaa acctggtgaa
    11701 agatacatcg aagtaagatg gctcagcgac ttcgcacgat agccagtttc ttcttctaat
    11761 tctctagctg ctgtcatttc gggattttgt ccttctcctg gatcgatttt tccagctgga
    11821 atctccaaaa tcactttttc taaaggtttt cgaaattgtt tgacaagaag cagacgatct
    11881 tgttcgtcaa aagcaatgat cccgacaccg ccaggatgaa ataccagttc tctcttcgaa
    11941 gttcctccat ctggcaaacg aacttcgtct acagcaacat caataatttt tccgctatag
    12001 atttctttgc gactgattgt tttttcttca aattgctcat tgttcagcat atcttctcct
    12061 gctttctttg taatcataac tgcttttttt tcatttttca atttttctca aaaactcctt
    12121 attagtttac catagataaa ccttagattt tctctaaata tagaaaatcc ttctttagtc
    12181 cgatactctt tctagtagtc cgtatgctaa aataagtatg taatcgaata acgaaaagaa
    12241 ttcaatactc aacgataaat gacatggagg gtttattatg ataaaacgaa attggccgtg
    12301 gcttctagca ttggtcttgc tatttctctt tgtccgaaac aatagttttt cttttgttct
    12361 aggactttta ttgataggtg tagttttagt tttgtgggga ctctttggtt cgcgcaggaa
    12421 aaagggaaaa caagaagaaa ttcctgcact gactgacgaa cttgaaactc actatgaaaa
    12481 aagcggtatg acagcgaatg aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa
    12541 tgagatcaat caattgcaaa tgaatatgca gcagacagcc aaacttaaag caatcgatct
    12601 ccgccatgat ccagtcaaag cagcgaaagc tttattcaaa gaattggtga aagaaccaaa
    12661 ccgactgcat gaagcaagtc agtttcttta tacgcatctg ccgaatctag ttgatttgac
    12721 aaataagtat aatgagatca atgaccatga gatcaaaaac aaacagacct atgaaaaact
    12781 agaagaaagc gcgcagatca tcgaccagct gtcagcttta atcgcacaag attatcaaaa
    12841 attcgttgct gaggatctag atgatatcga cgtagagata tctgttgcga aacagagctt
    12901 aaagcgtgat aataaatatg aaccagaaca aaaggattaa ctcttttgtt cataaaagaa
    12961 gactcggaac attcagatga tgtctggctg tttcgagtac tttttctaaa gtagagacat
    13021 tcatgtaaga atcgagaaca gattcaacaa ggaggaattt tttatggatc ataaaccgac
    13081 tgaaacaaaa gatgttacac cagttaacga tacacttgaa gatttattga acaacccgtt
    13141 ttcaactcca acagatacgc tgaccacttc acaacaagcg gagattgatc agctccaaga
    13201 gcaacagaca gctgctcgtt taattgacaa actaccggcg gaacgccaag aacaagctaa
    13261 acagctagca gcaaaaatcg atgctaatga tgcacagtct gtcatcagct acggatcggc
    13321 tgcccaagca aaattaagtg aattctcaca atcgatgttg aatcatgtgc aagcacaaga
    13381 tattggtcct gtaggagatt ccttaactga actgatgtat cgtctccaag aagccaatcc
    13441 tgacgaactg cgtgccggag aagggaacat cttccagcgt gtcttcggga aggtcaaaca
    13501 atcgatctat gaagtgacag caaaatatca aaagattggt gcacaaattg acaagatttc
    13561 agtgaaatta gataaagaaa aagacggtct gctaaaagat aatctgatgt tagaacagct
    13621 ttaccagaaa aacaaagact actttgatgc actgaatatt tatatcgctg ctggagaatt
    13681 gaaaatggaa gaacttcaaa caacgatcat tccagaagcg atgaaaagag cagaagaaac
    13741 tggcgatcag atggatgttc agattgccaa tgattacacg caattcctag atcgtctgga
    13801 taaacgtacg catgatcttc gcttagctag acagatcacg atccagcaag caccacaaat
    13861 ccgtttgatc caaaacacaa atcaggcctt ggctgaaaag atccaagcat ctatcgctac
    13921 agccattccg ctgtggaaaa accaagtcgt gatcgcattg accttgcttc gtcaaaaaga
    13981 tgcagtaact gctcaacgcc aagtctctga aacaacaaac gacttattga agaaaaattc
    14041 agaaatgctg aaaatctcag ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga
    14101 tatcgagacc cttcaaacca ctcaaaatga tttgatcgaa acaatccaag agacattgcg
    14161 tatccaaaaa gaaggaaaag aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga
    14221 agaagatctg aaacaaaaac tgttggattt aacccaatag atcacctttc gcttgttact
    14281 aaaacaaacg cccaacaaca gtttattaca actgttgatg ggcgtttttc ttatctttct
    14341 tctaacttct ccattggagt ctatttctca ttcatcttgc tgtgtccata gccatagcca
    14401 aaataaatga tcagtccaat aaccattacg ataccgaaca cgatccatgt cacagtttgc
    14461 agacgaagca tcaatacaat acaagtcaag atggagacga ttggcaatag cggaacgaat
    14521 ggcactttga attctccagc ttttggtttg ccaaacattt ttctcaactt cagtaagcct
    14581 agactaacaa gcatcaaata ggcgagcgcc atgatattcg ttagttccgc taatacattc
    14641 aatgggaaaa atccggcaaa gaatacagct gccacaccag cggcataagt cgcttttttc
    14701 ggtgtacgat gcttttgatc gatttctgtc ataaacttag gaagtaagcc atccttgctg
    14761 acagcaaaca gtaaacgagc aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt
    14821 aaaattgccc ctacagcgat caccgcgccc actgttccat gtcctacata cctcattgca
    14881 aaagccactg ggtcattgac tcctagttca gtatacggaa cgattcctgt caagatcagc
    14941 gtcacgatga tgtaaaggac agtagcaatc agtatcgaac caatgattcc tcttggcaca
    15001 tctttttgcg ggtttttgac ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa
    15061 gcaaaaaaca caagagctgc accatctagc acaccggaaa aaccaaaagg catgaatggc
    15121 tgccagttat caggtttaac gaaaaatatt cctacaatga taaacaacgc gatgatacca
    15181 aatttgacat ataccatggc attattcaaa cgtaatgctt tcttggcttc aagcgacacg
    15241 atgtaagtga cgaatatcac tacgagcacc gcaatcaagt caatgtatgt accattttcc
    15301 ggattgtacc cgccagttaa tgcttgtgga agttcggccc ctaaactttt caaaaaacct
    15361 tgcatatagc cagaccatcc ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc
    15421 aacaaccacc ctgtcagcca ggcagcaaat tcaccaaata caacatacag ataggcatat
    15481 ggcccaccaa tcactggcac tcgcgaagca aattctgcga aacttagtcc agacaaaatg
    15541 acaacgattg cagctaagac aaatgaaagt gacagtgctg gaccagcatt ttgattggcc
    15601 gcaacccctg tcaccacaaa tattcccgta ccgatgatcg caccaattcc aagcatgatc
    15661 aaatcagacg tttttagttc ttttttcatc tcactcgtat gattgagatt gatctctttc
    15721 ttacgaaaca aatccattga agttcctccc cattttaggt tggaaaacag cgattaaact
    15781 gttttcatat accctaaaag gataccacct aaaatgacta aaaggcaacc aacaataaca
    15841 taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc taaagtagag
    15901 ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga tttgactgtt
    15961 agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt tttccacaca
    16021 aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat cattccgata
    16081 ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat gatggtatat
    16141 acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa atcaagcatt
    16201 ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt
    16261 acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt ccattcatgg
    16321 aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga caatggtagc
    16381 cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc aacactccaa
    16441 gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg actaacaacg
    16501 aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg
    16561 gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa catagggact
    16621 aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag aaacttatca
    16681 gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct aacgtttttc
    16741 attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc cttcagttga
    16801 ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat aaagtcctaa
    16861 tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct tatcaaagat
    16921 ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa ggctgactcc
    16981 ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat atttgatggc
    17041 attactaagc aattggttga agataaaagc gatccattta gcatcggtca aaacagtatg
    17101 atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg cctgactgcg
    17161 tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt cccgcgagaa
    17221 actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt ttgataattc
    17281 attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa
    17341 tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat aatctttttg
    17401 gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga tcagctgatt
    17461 gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat aattttgcaa
    17521 aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc tcaagtaacc
    17581 acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag caattgtgct
    17641 caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg caataaaaaa
    17701 cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat tcatcttaac
    17761 cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat atatccttgg
    17821 atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca
    17881 tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact taagatcttt
    17941 ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa atcaatgatc
    18001 tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt gtggctcatc
    18061 atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca
    18121 atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat gttcatagcc
    18181 atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac tttcgatact
    18241 tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattatt atctagtaat
    18301 acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc ggtcgttcca
    18361 aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat gatctcttcg
    18421 tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct tgctactaca
    18481 atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat tttagcaaac
    18541 gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat
    18601 ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg ttgtttttgt
    18661 cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa tatacctaga
    18721 aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt tgaagagact
    18781 tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa
    18841 ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa tgtatttgtt
    18901 tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg accatacact
    18961 tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag ttttttctca
    19021 gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt tggaaaagag
    19081 gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc atcaaataaa
    19141 ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg ttcgtaatat
    19201 tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt gacttgtccc
    19261 attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg cactgctaat
    19321 tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt ctcgatcctt
    19381 ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa aactgcagct
    19441 ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg ggtttgaggt
    19501 ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac tgcagcaacc
    19561 tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact tcattttaga
    19621 tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta agaatgccaa
    19681 gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta ctctttctgg
    19741 ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat aagagatgat
    19801 aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg ccatggtctc
    19861 tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat ttccatctgc
    19921 gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc ctgattcata
    19981 aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt cttcattcca
    20041 caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt tttcaaactt
    20101 tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat caaattcatc
    20161 atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt
    20221 ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg ctaatctacc
    20281 taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga caaacccgtt
    20341 gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac
    20401 ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga ttttgtaatt
    20461 tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa taggtgctgt
    20521 gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg tttcttgttc
    20581 tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat tcatctgttt
    20641 atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt atgtgtcttt
    20701 ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg gcatcataac
    20761 aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta agaaaagata
    20821 gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga ataccgcttc
    20881 tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta aaaaccaact
    20941 ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac cgtcttctcg
    21001 ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa
    21061 atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca atccgctatt
    21121 cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg tcacttcact
    21181 tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa tccctattcc
    21241 cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat tcattttttt
    21301 ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt cctagtaaag
    21361 aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct gattgacctg
    21421 gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg gtctcgttat
    21481 aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat tctaattcag
    21541 ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc acccaaaatt
    21601 cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct tccacacctt
    21661 ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc gtaacaaggc
    21721 cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca ttgtctgctg
    21781 gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa tatttccccc
    21841 cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct ttttcagttg
    21901 ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg acatttttag
    21961 gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa attggcaaaa
    22021 tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact tctggatcaa
    22081 tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta ggtgctttga
    22141 tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt accgaagtcc
    22201 ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg ttcgtttcag
    22261 cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt tcatgataat
    22321 aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc tgtgaaatga
    22381 tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca tggtattcaa
    22441 attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat tccacgacat
    22501 cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca agcggtagta
    22561 aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc cctccttttt
    22621 agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga atgcagcaag
    22681 caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt caggatcgtt
    22741 cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt ctaactcttg
    22801 ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa cttttagtgt
    22861 acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt ctcgataact
    22921 aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact tcacttcctt
    22981 gatccgatag accccttcca acaagtctga agcattgaat agggaagaat cgtttggatc
    23041 tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt tctcaattga
    23101 aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa gttccaaatc
    23161 gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga tttcatagcc
    23221 ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa acttaccttc
    23281 ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg gtgcgacaga
    23341 ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat ttaaatcgac
    23401 atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca aacgtttcga
    23461 ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg aagaatcccc
    23521 cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt ctaattttct
    23581 aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc ataaacgatc
    23641 ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt gcttgagttg
    23701 gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt catcaggttc
    23761 tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt tggtagtttc
    23821 ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa ctttctcacc
    23881 atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc tccctttctc
    23941 ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga tttccagtcc
    24001 gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat tactgccttc
    24061 aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt ccagtttgac
    24121 atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag aaagcttgtg
    24181 atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga ctctcgaacc
    24241 agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt tggctgcata
    24301 ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg gtccagcgat
    24361 ctctctgatc caaaatatgt cgctgactgc tccagtttca tctcctaaaa tcggcatctt
    24421 cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg gttcggcttc
    24481 cccactctct ggattttggt catataatgc aaattcgact ccagccaacg gctcgccatt
    24541 ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa tggaagaaga
    24601 tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt tctgaatata
    24661 ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa gttttacttg
    24721 gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt tataagcttg
    24781 gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg cttgtccatg
    24841 acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc ctggatctag
    24901 atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat taaaaccagt
    24961 ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga tatcctccaa
    25021 ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta cgccatcttc
    25081 ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc gttgtctccc
    25141 gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt tctcttgata
    25201 cttgtttttc agccaatgat gataagcaac tgtgttgatc gcagatgact tttcttgcga
    25261 agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt gagtcgcttt
    25321 attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat cttctggacg
    25381 gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag tattcccaga
    25441 aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat cttccatcac
    25501 agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa tcggtgcata
    25561 actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat ttctattttc
    25621 tccgccattg tctttataca atgtttcata gccattttta agccctagtg taagcggagt
    25681 tcctccgatt ggtgtacgat ttaatgctgt attctcactt cccgtcaaac gattgatatc
    25741 atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag ctgtactatt
    25801 atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga gcagatcttt
    25861 actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat tggtcccagc
    25921 ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga ttggactgat
    25981 ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg ttgcatcttc
    26041 aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt ttttgatgta
    26101 cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc aaagctgaac
    26161 ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat tagtagcata
    26221 gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga tcaagttctc
    26281 tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg tccaatttcc
    26341 taacactttt gtatagtcat tcgttgtaaa cttcgggtag cgatgctgtt cgttttcaac
    26401 ttctccgcta ggatagatat attcataggg gtcttgcgta gctgttggcc caagacgctc
    26461 caaaataccg ttgaataact ggcgttgaat gcgctggagt gcttgaccgc tgggtacgat
    26521 tggttcgtcc agcttttcca tctcattttc ttcgctacgt tgaatttcag cggaatcttc
    26581 tgtatcagtt tcattttctt gtaattcttg gttatctatt ctttcatcaa ccaccacttg
    26641 aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc
    26701 ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat cgatcgtctc
    26761 gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac gaacgttttt
    26821 tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac cttcattggc
    26881 actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt caatatactt
    26941 cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg tttctgttga
    27001 ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg gtccaaatag
    27061 caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat ccttatactc
    27121 ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca tctaattata
    27181 atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa aacaccaatt
    27241 atacaacatt tattattaaa attaaattat aaaattttat aacgttctca tttaacaaaa
    27301 cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa aaaaataaag
    27361 cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga taacggcagc
    27421 gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag taggataatg
    27481 taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg ctaatattcc
    27541 tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg ctgtatgtcc
    27601 tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac gatttttttc
    27661 ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa aaaaatagat
    27721 caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca agactaaaac
    27781 agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa atttcaaaaa
    27841 attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa tactatcatc
    27901 gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt tcttgcgtac
    27961 gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat aaagcaaaat
    28021 agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc aaatagaaaa
    28081 gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa gcttcttttt
    28141 gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga agcaaaaaca
    28201 acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa atctgataat
    28261 cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca taaagagaga
    28321 agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca ttttttttgt
    28381 aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa acaggcaaga
    28441 cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca atgatttcta
    28501 agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg aacctcctgt
    28561 caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa tcgtcgggat
    28621 caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt ggttctgaaa
    28681 aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata acagaggacc
    28741 taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca aggcaggttt
    28801 cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt cgatttcagc
    28861 agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa atatattgaa
    28921 cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc agccgatctt
    28981 ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca tcgtagaaag
    29041 aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc cataagcaat
    29101 aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata atgtatttct
    29161 tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc atacgatttt
    29221 ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca atgaagtaaa
    29281 caccatccaa agcaatggaa tgatcaaaat gattcctatt actgtcatga ggacataact
    29341 tagtattttt ttgtttgccg attcacactg gtctttttga aataatccat catttgccgt
    29401 cacctccaga ataatagacc cacttaggtg caagcttcaa attgacaaac gtcagaacca
    29461 acacgatcag aaacaggatc attgacatag ccatcgcata accagctacg aacgccttgt
    29521 tccacatatg cagattataa aaaagcaaag aatttgcagg tccgcctgtt ccatttttcg
    29581 tcatgacata agcttcttga aaaatttgga aagaaccgat cgtagaggtg atcacatcgt
    29641 aaaaaataat aggagtgatt aatggcaaag taatactttt gaactttccc caaacgcctg
    29701 ctccatcgat ttctgctgat tcatataaag atctaggaac attttgtaat gtagctaagt
    29761 aaagtagcat tgctcctcca gaactccata ttttcatcaa aatcaacgca ggtttcgtcc
    29821 attgtggatc aaacagccaa gctggtccag ggatatggaa ccagcccaga aatgtgttga
    29881 tcaatcctgc agatggagaa agcagctgca tccataagat gtagactgct actcctgata
    29941 agactgccgg taaatagaaa gctgtacgga atacactgat tcctttgatt ttttgattga
    30001 gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg tacattgaag atgacgaaat
    30061 aaacggtgtt gtataacgat ttccaaaata aatcatcgtg aacgaacatt cttttgaaat
    30121 ttgctagacc aatgaaattc atcttggatg tcaaattata atcggtgaaa ctaccaataa
    30181 ctgaaaaaag cataggccct agcataaata ccaagaaccc gataataaaa ggcgagataa
    30241 ataaataggc ccatcttgct tcttttttct ttcggataga taaacgcttc aaattttctt
    30301 gcctcctctg tacctagaca gtcaaaaatg gattattgat tttgcttgac taagttttct
    30361 actgcttttt gtgcatctgc caatccttct tttggtgatt ttgttcctaa aagaatctgg
    30421 tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat ctaaaggaac tggtgtgatc
    30481 accgtatttt taaagttttc gtccgccatt tgatagatca tttttccttt atccgttagg
    30541 ttgtcattat tcactaaatt ctcattggct gtcttgctcg ccatgatatc aaaacttttt
    30601 tcgccgaatt tttcttgtac ttctggagaa gaaagatatt tgatgaattc ataagaggcc
    30661 tcaggatctt ttgctccgta tggtacttcc aagacgaatc cgccacccca cgaccaatgg
    30721 cctgaacctt cttctttttc tgggagctgc atcacgccaa aatcaaaatc atccggtgca
    30781 ttttccatca aactagagta ataattgata ttttgggcac gcatggctac taatccggaa
    30841 ataaatggat cagatacacc agacccaaat tcggcttcta atttattgat cgtatttttc
    30901 ccataataat cttgccagtc ttggatccac tccaaggctt ccactttatt tttagtatca
    30961 atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt tatctgcgtt tagcgcccat
    31021 acatcagccc ctaaattcca taatggataa aaaccgatgc gtgtaaattc ttttccttct
    31081 tttttatcta gtttacgtgc tgtttcttct aattcatccc aagttgtcgg tagatcttgt
    31141 tcactgattc cagcttcttt aaatatcttt ttattataaa aaatgacttg tgtatctgta
    31201 ttgaatggga tcgcataagg atcaccttca tattctactg tatcccaaag ctgcggataa
    31261 aaatccttag aagtgtcttt ttctacatat ttggagagat tagttgcttg ctgtgcttct
    31321 gctctttggg caacggaatt tatgtcctgt acaataacat cgggtgcatt tccagctgtt
    31381 attgcagata aagatttagt ccagatgtca ccccatggct gatacttata atttacatgg
    31441 atctcgtttt gtgattgatt gaaatcctca atgatttctt cgatagcttc ttttcgtgca
    31501 ccggatcccc aaaaggacca gaaatcaata ttttttcttc catcacttgt tttttcagaa
    31561 gaatttgctg cacttgaact ggcatcgctt gcattacttg atccgcatcc tcctaataaa
    31621 aacaatgctg aacttgccag caaccctgta aacaataatt ttttcatatt ttacctccaa
    31681 attttaatgt aaccgatgcc ggttttaata taacgatatc attttgttct gaaaataaca
    31741 atttataagg tatgattcta aaaaaattat taatgatacc aatactttat ataattcgcg
    31801 agcgctttct ttgaattttt taatctttcg tagcagaatg agaatgtatt aaaaatagat
    31861 taggaggaat tttgatgttt agacaccaga aagaattgca gtttgaagca aaaccagatc
    31921 gtccgaatcc attaatcgcg aaatatctgc aggagctgat cggcggccaa tatggcgaga
    31981 tgagtgtagc tatgcagtac ctgttccaag gctggagctg ccgaggagaa gaaaaatata
    32041 aagatatgct gatggacatt gcaacagaag aaatcggcca cgtggaaatg ttggcgacaa
    32101 tggttgctcg tttattagag ggtgcgcctg ttgaagatca agaagcagcg atgcaaaaag
    32161 atccaacaat cggtgccatt atctctggaa tgaatccaca gcacgcgatc gtagctggct
    32221 taggacctcg tcctgctgac agcgtgggca atccatggag cggtgcctat atcattgcga
    32281 gtggcaactt gttagcagac tttagagcta atttaaatgc cgaatcacaa ggaagacttc
    32341 aagtagcacg tatttacgaa atgatcgatg atcgtggcgt aaaagatatg ctgtctgttc
    32401 tccttgcaag agatagctat catcaaaatc tatgggaatg tgcagtgaaa gaattagaag
    32461 aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg tgacaaagaa cgaaaagaca
    32521 ttgcttatga cttctataac ttctcagagg gagaccaaag tagtgaaggt tcttgggcta
    32581 gtggcaaagc actggatggc gaaggcgaat atcaatggtt gaaagaacct aagatcgaag
    32641 gcgctgcacc aaaattagaa ccagtaacac caaaaatgta tgggacacct ccattcaaga
    32701 aataaatatc tacccgtaga aacacccgtt ttttgttcca cacgctttgt gagcaaaact
    32761 gactaaagtc attaatcata accacccttc aaaagggtgg ttttctctag agctataagc
    32821 tcaaatcacc ggccagcgcc taaagacgct ggctttcata ttttatgttc aagccatatt
    32881 gcctttgcca gttttgccca cctcttaaaa ggtttatgta ctactcctta gcccttaaaa
    32941 ggatcgtctt actctcgtac actcagctta tctaacgcac tatcatgttt ttcttgttct
    33001 tgaatgtact ttttgatggt cgcttcattt tgtcctactt ttgtcacata gtatccttcc
    33061 acccaaaagt gtcgataccc aaatttatat tttagatttg cacaatctct tgatgatctc
    33121 ctgaatattg gctcgatatt gattataaat tatttttctt ctatactttg gtgtgaatac
    33181 aatgtggtat ttacacatct actttgtacg ggctaaacta tttcccttta ttgctccacc
    33241 cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc tcaattaggt ctaaagacaa
    33301 taaatctaaa aacccgctgc ttcactgaca gaaacaacgg gttttcagaa aaaaatgaaa
    33361 tgaaaaagat ctcttacggc aatctatttt atttttttga ttttttataa atggaaaatg
    33421 cttcaagaag tccaagaaat aaagccgtat aaaaacaaga acctatgaaa tctttattta
    33481 ttagatattc aacagaaatc ccgataacac tagccaacaa tatatttatc agaagtgtaa
    33541 aataaggatg ctttttatag aatagaacta gtgttgtttt catagactct cctttcttca
    33601 tatttccaat taatcacaaa ttcacggcat tactagaaaa attaaagggg cgcagaagtg
    33661 gaaaaatact cttcgacttt ccctacaaac caggccatct tgtgtttaat aactatacca
    33721 aatctaaggt acctctaaat ctaaattcca tttgtggcgg tgacctccaa tttttaactc
    33781 ttctatctcc catccagtat acataacctt gatacttcga ccacttatat atcgaacaca
    33841 caaataccta tccattgcag gagtactacc taacttagtt gctacttgca tctaaataac
    33901 agctgaaaag ataggactta ctcttagtaa agtatctgaa ctattaattt ttaaactatc
    33961 atttttatcc tcaactacat gcacatctgt tgcaacttca ctttctgtct catccagata
    34021 aatgattaag gaatcgggaa ttgcaatatc ttcagctgac gctggaatag ttgagctaaa
    34081 ttagtcatca tatctttatt ataaaataac caacagcaaa aaatacaatg aaaaacaaac
    34141 ttggaactaa tgtatcatta ataaatgttg aaaaattcag caactcatta ctgtatgatt
    34201 ttagcagccc aaccaacata taccctataa aagctaataa gatggttagg
//
LOCUS       DACEZN010000014        31562 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEZN010000014 DACEZN010000000
VERSION     DACEZN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.014"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            7..189
                     /locus_tag="IXH60_000505"
     CDS             7..189
                     /locus_tag="IXH60_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1989472.1"
                     /translation="MTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSD
                     YIVSVEIIEFVADKEE"
     gene            303..542
                     /locus_tag="IXH60_000506"
     CDS             303..542
                     /locus_tag="IXH60_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989473.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            609..848
                     /locus_tag="IXH60_000507"
     CDS             609..848
                     /locus_tag="IXH60_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989474.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            975..1664
                     /locus_tag="IXH60_000508"
     CDS             975..1664
                     /locus_tag="IXH60_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1989475.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            1670..2836
                     /locus_tag="IXH60_000509"
     CDS             1670..2836
                     /locus_tag="IXH60_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1989476.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            2918..3589
                     /locus_tag="IXH60_000510"
     CDS             2918..3589
                     /locus_tag="IXH60_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1989477.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3642..5618
                     /locus_tag="IXH60_000511"
     CDS             3642..5618
                     /locus_tag="IXH60_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1989478.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            5631..6383
                     /locus_tag="IXH60_000512"
     CDS             5631..6383
                     /locus_tag="IXH60_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1989479.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            6399..7160
                     /locus_tag="IXH60_000513"
     CDS             6399..7160
                     /locus_tag="IXH60_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989480.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            7173..7433
                     /locus_tag="IXH60_000514"
     CDS             7173..7433
                     /locus_tag="IXH60_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989481.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            7430..9520
                     /locus_tag="IXH60_000515"
     CDS             7430..9520
                     /locus_tag="IXH60_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1989482.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(9556..11613)
                     /locus_tag="IXH60_000516"
     CDS             complement(9556..11613)
                     /locus_tag="IXH60_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1989483.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(11758..11949)
                     /locus_tag="IXH60_000517"
     CDS             complement(11758..11949)
                     /locus_tag="IXH60_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989484.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            12137..12592
                     /locus_tag="IXH60_000518"
     CDS             12137..12592
                     /locus_tag="IXH60_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989485.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            12589..12831
                     /locus_tag="IXH60_000519"
     CDS             12589..12831
                     /locus_tag="IXH60_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989486.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            12849..13151
                     /locus_tag="IXH60_000520"
     CDS             12849..13151
                     /locus_tag="IXH60_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989487.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            13220..15274
                     /locus_tag="IXH60_000521"
     CDS             13220..15274
                     /locus_tag="IXH60_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989488.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            15274..17886
                     /locus_tag="IXH60_000522"
     CDS             15274..17886
                     /locus_tag="IXH60_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1989489.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            17883..20261
                     /locus_tag="IXH60_000523"
     CDS             17883..20261
                     /locus_tag="IXH60_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989490.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            20322..20864
                     /locus_tag="IXH60_000524"
     CDS             20322..20864
                     /locus_tag="IXH60_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989491.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            20942..21274
                     /locus_tag="IXH60_000525"
     CDS             20942..21274
                     /locus_tag="IXH60_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989492.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            21275..21868
                     /locus_tag="IXH60_000526"
     CDS             21275..21868
                     /locus_tag="IXH60_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989493.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            21890..23914
                     /locus_tag="IXH60_000527"
     CDS             21890..23914
                     /locus_tag="IXH60_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1989494.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            23914..25113
                     /locus_tag="IXH60_000528"
     CDS             23914..25113
                     /locus_tag="IXH60_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1989495.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            25129..25773
                     /locus_tag="IXH60_000529"
     CDS             25129..25773
                     /locus_tag="IXH60_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989496.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            25784..26590
                     /locus_tag="IXH60_000530"
     CDS             25784..26590
                     /locus_tag="IXH60_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989497.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            26613..26842
                     /locus_tag="IXH60_000531"
                     /pseudo
     CDS             26613..26842
                     /locus_tag="IXH60_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27001..27936
                     /locus_tag="IXH60_000532"
     CDS             27001..27936
                     /locus_tag="IXH60_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1989498.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            27980..28249
                     /locus_tag="IXH60_000533"
     CDS             27980..28249
                     /locus_tag="IXH60_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989499.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            28525..28800
                     /locus_tag="IXH60_000534"
     CDS             28525..28800
                     /locus_tag="IXH60_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989500.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            28842..29264
                     /locus_tag="IXH60_000535"
     CDS             28842..29264
                     /locus_tag="IXH60_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989501.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            29284..30876
                     /locus_tag="IXH60_000536"
     CDS             29284..30876
                     /locus_tag="IXH60_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989502.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30897..31232
                     /locus_tag="IXH60_000537"
     CDS             30897..31232
                     /locus_tag="IXH60_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989503.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            31378..>31562
                     /locus_tag="IXH60_000538"
     CDS             31378..>31562
                     /locus_tag="IXH60_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989504.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11359 a         4892 c         6432 g         8879 t
ORIGIN      
        1 caaacattga ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat
       61 gaatggttta aaatgacgct tacggatgat aatggagacg aattatctgt tgaagatact
      121 tggtcatatt tgagtgatta tattgtcagc gttgaaatca ttgaatttgt agcggataaa
      181 gaagaataaa aactggagga ataaatttta cagataaagg cttagaagga gtcacgacga
      241 ataataaaaa ggtagtatta ccttaggcat tgagtaagga atttatttag gaggaaaatt
      301 agatggcgcg accagtttta gaggcgatgg atatggatac tttgacaaaa ggaaggtatg
      361 tacagctgtt agctcgtggt tattcaccta aagatgtatt caaatccctg tctaaaggaa
      421 cagatatgga aaaagaaaga ttacgaaaag aattcgatta ttggagaacc cataatggaa
      481 tcaaggattt gaaaccagca cggccagtaa ttaaacgaaa gaaaaagata acccaagact
      541 gaagtgtaga gcgtatgtga tcataggctt ttttattttt gcaaaaaaca agaaaggtag
      601 gaaaactcat gaattatgaa gaaatcgaaa acagaaaaaa agtgtcgaaa gaaatggaag
      661 aaaagttgct aaaaatgatg aaacaaaaac accttaaaag attatctgtc atgcaatata
      721 tcaatgatat gaagataaca ggaaaagaga aagcttgttt gttaggttca atgaaaaact
      781 ttgaacagtt gaggcgaact tatgtaaaaa caggttccaa ttgccaacta ttattagaag
      841 tttcttgacg attatgatat gaaaatgatc aattatgatg tggcattgtt attgaataaa
      901 aagaatagta aaatttaaca tggtttatct tggttagatg taggtaaatt aattcatata
      961 tagaaggtag atagatgaat aaaaacaata aaactttatt taatccttac gtgggcgtaa
     1021 tcattgtttt tcttcttttt agttattttg cttatcaagg ttatcaaaag gacaaaatcg
     1081 ctgaaaaagt agatggtgta acaaaaacaa tccatattca ggaacaggct aaaacctatc
     1141 aagataattt aaccagttta acggatattt ctaagagaca actgattact aatcaaaaac
     1201 aggcattaga aaaggatctc aaaggtgaaa ttgctggaaa acttgttttt tcttctacag
     1261 gatttattct ccctgttttt caaggagcga atagcgacac gcttagttta ggagtagctt
     1321 ctacttatta tctagatact gaaatgggaa atggaaatta tattcttgct ggttataacg
     1381 tagatacacc aagcgtttta ttatccgata tgactgattt atctgaagga gaagaaattg
     1441 atctaatcga tgctcattac aagtatcagt atgaagtctc caaaagagga aaaatttctg
     1501 attacagcca ggtagttcaa tctaaggaaa acaattcctt tttatccttg ccaaaagcag
     1561 gtaaaaaacc actactcaca ctgttagcct ttcaagcaaa taatatgaaa aagtgcgatg
     1621 tgatccagtg tgaattagtc aatgtagaaa aaatcaataa ataagggaag tgagtcacat
     1681 tcaagaactt gtagaaaaat tgatcaataa aatgagtgcc atttttctgt tactgttggt
     1741 atccattcta tatttaatat ggacggtggg gttaaatcct cctacgatat tatttattta
     1801 tgataatgct gcacagttag tgacgttttt cgtaggctgt ttgtttcttt tgaatctgta
     1861 caaggtaaag agaaacgatc tattcttcat tgcagcaggc attctttttt accttttctt
     1921 ttcttttttt agaagctacc gaggagatac tttctatggt gatctgatcg ttccgacaat
     1981 tattgctatt attttgattg taaaatggac gaaatttact gtaattgatc gtagcttgta
     2041 tcttgtagta ttctatgtct ttttactcgt aacggtttat cgagtattta ctgagatacc
     2101 agtaccagaa ggacaaagta tttggaatcc atcgaacaaa ttatcagata tttggatcaa
     2161 tacaaatacg ataggtagtt ctttaatgac attagccctt cttatcacag gatttgctag
     2221 ttcatttgga aaatggtaca tgcgattgct atcattacca gcgttgattc ttgctggggc
     2281 aacgatttgg gtttgccaat cgaaagcagc attatatgcg cttgttatct tcattatctt
     2341 agaaatttta cctaagaagg tttttcgaaa tacacacttg tccttttgga tgtatgcagc
     2401 agtttcggtt ctagctttac cgatttctta ttttgctgca atgtcagaca aagttcactt
     2461 atttacagga cgagaagaaa tttggcgcaa attctatgaa acattaggcg aaaaaacagg
     2521 acaaatattt gtagggatga aacctttctt tttccataga ggaaatcaga ttctaggtaa
     2581 tcataatacg tatcacgcac tcgtcaatca ctatggattg attggttttg ttctatcgat
     2641 tattcttttg cttagtttct tatggttgct cgtaagaaaa cgtgagctga caaatggaca
     2701 actaacattc ttgtgggctt ttttagcgat catgtgtcaa tcgttcatgg aagaaacatt
     2761 aacgacaatc agttgggtgc caattgtatt cttaatgtta gcaatggcta cacaccaata
     2821 taaaaccacc agataaatga aatagaatag taataagaga aaacactttt tcgttgtgaa
     2881 aaagtgtttt ttgttttgtc tgaaaggagg cagatggatg aaaaaatggt tatttggttt
     2941 tttaggagtc gctttaatcg tcgtttgcag tgtgtttggc tatgttagct accaaaaaca
     3001 tgaaggagag gtgtttaagc aaaatataga aaaaaagatg cccgtagatc aaatcaatgc
     3061 acacgcaaag agttataagg aggatgcaac gaacgtcaat aatgatatgt ctttggggca
     3121 aatgttgtcc atacaaaaag aagcaatcga aatgggcgtc aataaacaag tatttgcaca
     3181 aattcagatt cctgcacttg gtttagcatt gccaattttt aaaggggcaa atcaatacac
     3241 tttgagtctt ggagcggcta cttattttta tgaagatgcc gaaatgggta aaggaaacta
     3301 tgtcctagca ggtcataaca tggaaatgcc aggtgtttta ttttctgata ttcaaaagtt
     3361 gtcattaggg gaggtgatgg acttagttag taacgatggc gtctatcgtt acaaggtaac
     3421 gcgtaaattt atcgtacctg agtactttaa attaatcgat ggcgttcctg aagagaactc
     3481 tttcttgtct ttaccaaaga aaggagagaa acccttatta acgttgttta cttgtgttta
     3541 tacgtcacaa ggaaaagaaa gatacgtggt acagggagag ctacaataac aagaatttaa
     3601 tcaacgcgag tcgttgaaat atgaaatgag gagagtaaaa aatggcaata aatcggtatg
     3661 taaaaaaagc tggaatgatt gttggaatgt taggaatcat tgcaggctgc tttcaggggt
     3721 atcgagctta tgcagaagat gtcacgcaaa aaacgccacc agaaagggtc aatatcacgg
     3781 ttcataaatt aatgtacgat caaagtacgc aattgaacgt tgatattgac ggaatcaaaa
     3841 atgatggtta tacgcacgat caatatccta caggtgtaac gaaatacaac aaggcagatt
     3901 atggtgatgt tgagtttacg ttaggaaata ttacggatca agtattaccg actgaagatt
     3961 ctgatctaac caacgcgaaa gtggacgaaa tcgttaaaga tgtagaagac aagggatcta
     4021 acagtgagta cgttaagaat gcaaccaata aaattacttc agctgtagat gaaaatggtg
     4081 agatcacttt tgctgatcaa ccagcctatg tcaattccaa agggaatgta tatgtggttt
     4141 atgaaagtaa atccgctgct ggattagtca cacaaaaagc aaaaccaatg gttgtgattg
     4201 cgccaatgac agacaacaca ggatcaggat ttttaaaaga tattcatttg tatccaaaga
     4261 acattgtttc taagttgagt tttgaattaa cgaaatttgg tgacgatggc acagctcaaa
     4321 gcaaacaaac acctttaaaa ggggcaaaat ttgagttata caagggcgaa cctggaaaag
     4381 gaacaaaatt aggagagctt gtatcggatg atcaaggaaa gctgactgca actgatttga
     4441 cgttaggaaa atactacttt gtggaagttc cttcagaagt tgtagttgga tcagatcaag
     4501 aaccaacagc agaccaatat ttgttaggcg cagacgctcg caacgatgcg cacaataagt
     4561 taacctttga aattacgaat gacggcgtga caagtgattt aaaagcttcc tacgtgaact
     4621 ataaagcacc agtgatcgat aaaacagtga caaatggaac aggagaagaa cattctttcc
     4681 aaattggtga cgcagtgaat tatcaaggta caattcatat ccctacagat atcgcaggtg
     4741 gaacagatgg gattacagta aatggtgtaa aaagtgaaac ttctccttac agtgtattta
     4801 aatggggcga cacagcaggt aaaggacttt cttatgttgc tgaaaaagca gatattaaag
     4861 taacaaataa tgatggttca gtcgtattaa aagaaaatac tgactataaa atcgaaaatt
     4921 ctgatagtgg ttttgtgatc gattttatcg taaataatgg acaagtaagt gatacagttg
     4981 cgaatttaca tggtcaagat ctaaaaatga cttataacat gtttgtgaat gatagtgctg
     5041 aaatcgcaaa tccattaaca aatagcgtgg atttcgttta taacaataat ccatttaatc
     5101 aggaagaaca ccacgaaaaa acgaaagctg acgttgtgac ttatggcgca aaattcttaa
     5161 aagtcgatag cggcctattt ggaacaggta tcaaagcaac accgcttgaa ggcgcagaat
     5221 ttgcagctaa gaatgcagaa ggtaagtact acggtggact tactgataca gataaagatg
     5281 gtgtcaaaga agcagtttgg gtagacgatg tagcaaatgc agcaatccta aaatcagata
     5341 aagagggtca ttttgaaatc actggtttaa ctgaaggaga ttactcactg gaagaaacaa
     5401 aagctcctga aaactatcaa aaattaacaa aagagatctc tttcaaagta gacaaagatt
     5461 catacaaaga agaaaaccga atcacgatca aaaataatca aaaagcttcc gtaccaatga
     5521 ctggtagcaa cggtttccaa tcttacgtat tgatctcttg cttgttgctt ggggcaggcg
     5581 caatttctgc agttgtttac tttaagaaga aagcctagaa ggatcagcta atgattatca
     5641 gacaccctaa gaaaaagcga atcatgggta aatggatcat tgctttctgg ttactttcag
     5701 ctgttggtgt cttacttttg atgcctgctg aagcgagcgt ggcgaaatac caacaaaatc
     5761 agcagattgc cgcaatcgat cgaacaggaa cggcagcaga aacggattct tctttggatg
     5821 ttgctaagat tgagttaggc gatcctgttg gtattcttac aattccaagt atttctttaa
     5881 aactaccaat ttatgacgga acttccgaca aaattttaga gaatggtgta gggattacgg
     5941 aaggaactgg cgatattaca ggaggaaacg gtaaaaatcc tttaatcgca gggcacagtg
     6001 gactttataa agataatctt ttcgatgatt tgccttcagt taaaaaggga gaaaagtttt
     6061 atatcaaagt tgatggtgag cagcacgctt atcaaatcga tcggattgag gaagtgcaaa
     6121 aagacgaact acaaagaaac tttgtcactt acttagaacc aaatcccaat gaagatcgag
     6181 taacgctaat gacatgtacg cctaagggga tcaacacaca ccgtttccta gtgtatggga
     6241 agcgagtaac ctttacaaaa tcagaattaa aagatgaaga aaataagaaa caaaaattat
     6301 cttggaaatg gcttttagga agtactgttt ttctttcagt catgatcatt ggttcgctat
     6361 ttgtttataa aaagaaaaaa taaaggaggg taaccaacat gagaaataga aaatggctat
     6421 ttgtttgtgc agccgctctt ttttctttca ctgcctacac acagttgacg tttgcagaca
     6481 gacaagaaag cacggatagc acagaacaaa caatccaaac gtcagatagc agcacgattc
     6541 aaagcagcga tgaaaccgtc gattcgtcaa cagttgattc ttcagaagaa cctaaaattg
     6601 ctgaagacga tcaaatagtc aaacagacca ttcacgttca aaaaattatt tcttccgaac
     6661 aattagacaa ccaggggcaa aagcttgaaa aacaagaagg agtaaatggc gcgaagtttt
     6721 ctgtttatga tgtatcggac attttacaaa agatggatgt taaggatctc actactgacc
     6781 aaattgaatc ccaattaaaa gatcgagtga aaaaactatc cagtgatcaa ctgaagctaa
     6841 tttctaatgg agaaacaaag acaattgatc aacaaacagg tgtctttgaa ttctcggtag
     6901 aagtacaggc aaatcagaaa caagcttatt atattgtcaa tgaatcaagt cctgaaaaca
     6961 tttctaatag cgaggatatt ttgctattaa cacctgtttc tgataaaaat ggtgaattct
     7021 taaaggatgt gtggatttat cctaaatcag aagcttcgca acctaaagaa gaagtaaaaa
     7081 aaatcgtatc tactggcgta aagaaaaact tctttgagaa ttgttgggat tttgttactc
     7141 acttgttttc tagagattaa ggaagaaata aaatgaaact gatcaaatta ttattattca
     7201 atttttcttg tttaaccgca gtctttttgt actgcggttt attattttct aaactatcta
     7261 ttttgccaag ctaccaacta tacctaatga ggattttaaa cataccagct gcttccatgc
     7321 cgctagatat tatggaaaag ctcattagga atccattatt tactacgatc aatttgatcg
     7381 ttattgtcgg aatttcacta gtcatttata aaaaagaggg gatgaaggga tgaattttaa
     7441 acactcgtgg acaagacggt taaaatatgt ttttcccata atgatgatgt taggtacatt
     7501 gtttgggtta aaaacagcca atgtctttgc tgaaaaagtt attgtcgatc cgtcaaatcc
     7561 aatccagaac gtaaagaata attttattat tccaaaagat gttcctaatg caaaaacaaa
     7621 tattactgta aacggagcgt caaaagttta caaatatcat gttgatgcca acaaaggtgg
     7681 ttttacagac gactacgtag gattagtgga gggaaacaaa aatatacgct atccttcttt
     7741 ccaaaaagaa tacggtgaaa caaaccttgt tttagaaggc gtaacaacca acaccaaagt
     7801 caatattgac tatggaaaaa tcggtacgta taacgggaaa aaagtcaata tcaagctagt
     7861 tctatctaat attcacttat actcggatac gttgccgtgg aatctactag ataacaacta
     7921 tacaaaaact cattttcgag acgatggcta taaaaatacg aatggtgcaa tgagtaaatc
     7981 taaaaaaaga acagtacttt ggatctcaga caatcttttc tcaggaattg tttaccattc
     8041 aacacaaatg aatgtgcagc tagtagccac atatgaggac ggtagccccg ttcaattttc
     8101 aggagataca tttatcagtt ttaactcttt gaaccctgca ggaggaaaaa gcacggatct
     8161 taaaggagaa tatgcccact atgacaaaat gaatacgaca gattggtatg tcagaaaaga
     8221 tacggttctt tcagaattta aatctttcta caacaatctg aacgtggtag gtggccaccc
     8281 aggaggaagc tctaagctga cacaagcgga taatgatttc aataatttgc atgataaatt
     8341 aggagatccg aagtttggtc aaggtactgt gtcctttaaa atatctgaag caaatccgac
     8401 gtttgtgatt ggttcttcta atgttcaaac ctggtttact ttatcttctg cgacaatctt
     8461 tagtgttgtt cctgatcaac ctgaaaaaac aggggttgat aaaaatggaa ataacgtgaa
     8521 cgataaaatg ttgcaagtag gagacacgat tcaatatcga atcaaacaga aggtgaatcg
     8581 attaggagtg gatttactag cggtttacga tcgatttgag ttaatcgata atttaccaaa
     8641 agaagtaaat tacgtcgatg cacacgttga aagtggtaca aataaaaaat tcgatgtatc
     8701 tggtgaagtg acctatgaca aaacaaaaca ccaggtaaaa tatgcagcga aagctgatac
     8761 attaaaaaat cgaatgaagt ataacggcga aacttacgaa ttagtgatca atgtaaaagt
     8821 caatgaattg gccaatcaaa atagtgtagc caaaaatcag ggaacgtcga tcattaataa
     8881 agtagaaaag gatacaaata tcattaccgt ttatttccct aaaattccag tgaaagaagt
     8941 acaacaaaat ggaaaagatg taaacggacg aaatgacggt aagaagggta cgccgacagc
     9001 tcctttaaac gcaggatctg aagtacaata tctagtcaca caaaaatggc acacaaaagg
     9061 cgtagacgct gcgtcagatc actacaaaca atttagtatt caagatccaa ttgaagctcg
     9121 tttgacatat aaagaaggtt ctgcgcaagt aatagataag agtaaaggaa aagatattac
     9181 ttctgaaggc acactaacct atgatagcaa ttcaagaacg ctgaagtggg aagcttccgc
     9241 tgacttctta aataacaatc ttttagatgg acgagaaatt cagctgatat ttacagctaa
     9301 aactccatta caatcagaaa aaaatattga taaccaagcc gtagtagcag tagataatgt
     9361 ttctaataaa acaaatgttg tgacaattgg ggttgatcct aacttgccac aagttattgt
     9421 tcctaaaaca ggttctacac atttagtaac gattttagcg gtcagcttac tattacttgt
     9481 gttagcaact tttggctacg cagttctgaa gttcaattaa acaaaaaaac aaggctgaaa
     9541 atcagccttg tttttctatt tgaaaatggg attggcacta ataaatacgt tgtctgaaga
     9601 aagtgatccg tctgcagaaa cttcataagc atgggataaa ccaacgatac tttgattgtt
     9661 gtaaagcaag tttgcgttat gagcagcgct atctttccac ataaccatag catttactgc
     9721 tcctgtatct gaaaggatct cgtcaacgta aaggtagaaa ctattgatat tttctccttt
     9781 agcaccgtag cagcctaagc tttctaagag atcttggaaa gtaccgccgc caggtcggta
     9841 atgactgaag cttacttgta attcttttgc acgaatatct gcagcttgtt gaacgatatc
     9901 attgaattgc attggggcaa cgccattttg ttgacggtag ctatttaacc aagcaagttc
     9961 tttttgacgc acatagttgt ttaaatcact tagtgagata tttttgtatt tgaaagtgat
    10021 cgttcgattt tggtcaacat tattgattac acgttgtcca tttttatcaa cggtaacaga
    10081 tgaatcgtca tttctacaca agaagtagtt ttgatcggtc atatttttcc aagaagtaga
    10141 gaaactatct ccctcgtaaa ccgtttgtgt ttcagtagat aaaacagcgt ttgtgtcaac
    10201 gtttatatgg ttcacagtga cgttgtattg atgagtatag gcaaaattga ttccttgatt
    10261 acttgtaacg ttgtttaaag tcacagtgtt gctagagctt tctctaggat caagaacaaa
    10321 attagtgaac ataggtgctt caattgtgat gctttgtcca taaacgacgg gataactctt
    10381 actgtaatta tagagttctt ggatatttgg atcagtatcc gagactttat ttggacgagc
    10441 tttcacttca actagaaaga cttcatcata gtaaaaagta aatgtggtat ttgtgttaat
    10501 cgtttgtgtt tgtgtttcat tcccatttac ttgatactgt ggtgcttcaa agttagtcaa
    10561 gtcggttcta gcttttgcag tgtaacttgt accagctttt actgagacat gttcttcttt
    10621 tagcagcgtt ccagtttgtc gattcatatg acggacagtt accgcatatt ctgaaggttt
    10681 aggtttgtgt tgtaaccctt taaatgcaga atcaagacta tttttagcat tatttacttg
    10741 ggtttgagaa gctttagaat cgtctagtac ttttttagca ttggataaag cagtctgaaa
    10801 attattccaa gatccttctg taaaatctcc tttagcagtt gattttactt ggttgtacaa
    10861 ggcttgtaat tccgttttgt tcacagtagg agctggtttt ttctttaatc ccttgtaagc
    10921 agaatctaat gtgtttttgg cactatccac ttgtttttga gtcgcttttg agtcatttaa
    10981 cactttttta gcattgttta aagcggtttg gaatgcgttc caggtattgt cggtatagtc
    11041 acctttttta gttcccttaa cgctcgtata aagagcttct aaagctgatt tattcacttt
    11101 gggtttagag gtgtcagcac cataccaacc aataccttcg tatttccagc catagtcttt
    11161 ggttaaccat tgtacttctt tccaacttgc agtgtagtga tgcgaaccag aggtaacacc
    11221 tggaaggaaa aggcggtaga tcggtttatt atttttttcg gcagaacgcc aggcaggccc
    11281 ttcataagtc caaccgtaat ttttggtcaa ccatttcact tcatcccaac tagctgtgta
    11341 atggtgatca cgaaggcctt tattgtataa acgatagaca aggtggccac cgctagtcgg
    11401 tgcttcccaa gcggggcctt cataattacc ccacttaatt ttcactaggt gatcacgttc
    11461 ttttgtgcta gaggtgtaaa aatgttcatg gttatttgga ttataaaggc gatacatgac
    11521 gttagttgaa gcagcgtcag cagatccagc aagagcaact gtggcagcaa atgttccaat
    11581 agctataaca gtttttgggt ttaataattt catgttttca actccctatt aaatgattaa
    11641 atattttgtt tacattcata atataccacg gtataccgag gtagtcaacg gtataccgtg
    11701 gaaattttga aataaaaaag ccgccctatg aagagtgtaa ggatggctgt ttatatgcta
    11761 tgaagaaatg gattcggcgt atttggttag tttggcagca ttatcaaatc gcattttttc
    11821 aatcgcactt tttttcttta ttaaatccgc aattgttgat tctgcgactg aagaatgttt
    11881 gctaatttca tatgctgtct tgccagtttc aaatagtaac cattcgattt tctctttatc
    11941 agcgtacatt gcaatgctcc ctcttgattt tttctttatt atagctcttt tttgtaataa
    12001 atcacaatat caagaggaaa agtttctata gaaaggtggg aaattatgag tatccgttcc
    12061 ccgtgtatca aaatgaggtt tgttgctact ttcgttattt aatatactaa aataaaacaa
    12121 cgaaagaagg aatagaatga aaaagttaat aatggtattt acttcgttga ttgccatgat
    12181 tgttatttta ggtggatgtg gtagtgaaaa aaataagcaa gatagtcaag ataatggcgt
    12241 gttaaaacca ggaacagtgt ggaaagaaga agtaggcgga cttgttcata atttaaaaat
    12301 tatagatgat acaacatggg aatattccga aagtgtttgg catcctgaac aagtacaaat
    12361 aacagttgag aagctaaaag actataaagg attagaaaga tataaggtaa tcgattcagg
    12421 tggagtagat ttattcatag gtaaaacagc cttaatagtt ccctataaca aagacggttt
    12481 agaaacgttt aaatttattc caacaaacga aaaagaacat cgagataaaa gtgaaatatt
    12541 aaaagaatac agtactaaat ctggatacaa gttacaaaag actagcgagt gattgctagt
    12601 ctttttattt atcctgaagg aggaatcgca gtggcaaaag ctaaaacgcc atttgaaaaa
    12661 gccgcgtcta aaatcttaga acgtcgcggt gtatctgaaa cacagtggaa acaagaagtc
    12721 cttgaaaaag cacaatttga cctattacgg ggaaaagaca aggacttaga aggatatatc
    12781 tatcaacgtg aacgtgaaaa gcttgttttc gatgaaatta ttaaacacta aaaggagcga
    12841 acaattttat gtttgaatca attacaatga attttttaac acaaccgaaa gtagtagctg
    12901 cggttgatat ttttagagca ttacaaggtg cagcaactag tttaaaaaac aatctgatga
    12961 tttttgcggc tgtagctgcc gttgcctcca ttgttcttgg ggggattttc ttcttctttg
    13021 gccgaagagg agctgaaaac gggaagatgc acgtaggtta tgtattgatg ggggtaggag
    13081 ttatattagc tgccgcctct attgtggttt cgttatttgg aatgtttgga caaaccccac
    13141 agggactata aaaaataaat agaaggagta aaaagtacgt ggctactttt tactcctttt
    13201 ttatgaaagg agaatactca tgtactttac gatttcaggc aataccaaaa aagataaaga
    13261 gttaataaaa aaagcaacga agaaagtggg aaagcgtact gcctttttac cgaaaacaat
    13321 taaatattct gtcattgaaa cggaagaaca gctacatgtg ttaggacaag tgttagaaca
    13381 agagaatcaa tcttctgtta aattgactct cacgttacat gataaagaag gaaaaataga
    13441 aaaggctggc acagaatacg tttttggccc actggattat caaatttcag aaggttttta
    13501 tcctacattg atggatttga tccgccttga tgtgtttaag gaaacggatg aaaccatgag
    13561 tgtggaagag tcttatcgaa acgaaaaact gctacaagtt ttagaagaag agttagagag
    13621 cgaccaaaga aaagtgagtc aagtgagtga agtcgaagta aatgatgaag taccatggga
    13681 acgaccaatc tttccaaaga aggagtcaaa agaaaaagag acagtctatg ttgacgaaca
    13741 agctgatatt ccgactcaga aatcgatatt tgaggaagag aacgatgtaa tgggtcaatt
    13801 ggaggtagat ccctttgaag aaacaatgga ggatgaagag acggccgaag tagaagttga
    13861 tctagttgaa gctaatgaag agccaacatg gactgaaggg gattctcagg aatatattga
    13921 accacttatc agtgaagaag tagagaaaaa cgaaacagag atacaaaagg caactgtatt
    13981 tgattatatt ttggatcaat atccgccaga gcatgaatgg atgaaagaaa aaaataaatt
    14041 atttttaaat gagctttatt cgaaatatca attaccccaa acctatcaag catttttaga
    14101 gagtaaagaa gaattaatct cacagggtaa aggcacactt ttagaacagt tagagtctgt
    14161 tcaatcaaca aattggcatg agcttgcaca agaagattta ttagccactt ttgaagaacg
    14221 tcaacaagaa acagatcaag aaataaatga gtatgcgacg gaacagtcat ctaattggga
    14281 gagaacaaaa gaggaactaa ataaagaaga acaacgagtg attgaagaag aagtacaaaa
    14341 aattaaagag cgatacgaaa agaaacgcga caatgcgtat tccttgtgta tagagaaaat
    14401 taataacttt actaaaacta atcaagaagc tttatcgaaa gaaaaagaaa acttgttaaa
    14461 agaacgtata aaagaacgaa aagaagagtt ctaccgaggt ttacgtacag ataagctaaa
    14521 gattagcaaa gaattaaatg aacaactgaa taaactttat caaacaacga ctgaagttct
    14581 tatggaaaga cacgggcaga tccagcaaga attacaagca caaattccaa aatggaaaaa
    14641 agagtatgaa attcaacaac aagaattgaa agaacaagaa gaaaaacaac gacaagaaga
    14701 aaaagagcgc gaacaattag aactaaaaaa acgtgagcag catcttgcag aacaaaaagt
    14761 ggcgatagaa aaacaacggt tagagcaaga aaaggaaaaa gaagcagctg ctcgtaagga
    14821 acgtcaagaa caaatggata cattgcgaca agcacagttg aattatctag cacaacctca
    14881 accagtagct gctgttcaaa cgccagtgaa caaccaagca aatttaactc cttcaccaca
    14941 gcgttccttt aacggatgga tggtgggatg tattgcaagt ttaagcttgc ttctaggtgg
    15001 aggaacagta tttgctttta atcatatcaa tcaatcttca caggcagatt ctgtagccac
    15061 aacggcgtca atcaaggaag aagcccagga acaagcaaga aaagagtatg aacaacaatt
    15121 ggaagaatta ttgaatcgta ccaatcagtc aacagcagag tcaagtatgg aaaatagcaa
    15181 agagagtgat cctagtaagg ctaaagaagg atcaagcgat tcaactgtac actcaaacac
    15241 ttccgaaagt tcagaatcaa atgaggtgaa ataatgcgta ttagagaaac aacgagacga
    15301 aaagacaaac gattcaattt agaaaagaat aggtatcttt ctcatttcta tcgctatact
    15361 ccttcaggag aagagctacc agaaaaacat gacaaacgta tttctcaaaa gattttttat
    15421 agtggcttag cttatcttcc tttaaactat gttgttacaa gaataccaga agcatttcat
    15481 cgatttgaga ttattccagg aagtttacaa ggagtgaata tttttcagtt acaagaaatg
    15541 ccgcagttat tccattttga ttcaactggt ttttggaatc ctttgacccc aacaacaagt
    15601 ttaggcacac gtttagttgt ttctacgatt tgtgttgttc cagctgtact tatgggattg
    15661 aaaatggaca tgtggtttcg tccgctagca gatgggcaaa aaggagataa ccgattaaaa
    15721 acgttaaagg aaatccaaca aatttatccg caaataccag aatcaaaggg aagttttcct
    15781 ggttatggcg gaataccaat tactcattat aaaaaatact ggtatattca gacggataca
    15841 gtaaatactt gtattgttgg tacttcccgc tcagggaaag gccagataga agttttagca
    15901 accattgaca atttgtcgcg tgcagaaaga caaagttcaa tggttgttaa tgatccaaaa
    15961 acagaattat atgttgctag taaagatgaa ctagaagcta gagggtatga tgtgtatgcc
    16021 tttaatatca ccgatccact gcaatcaatg agtgataatc cattggcact cattgtgaaa
    16081 tactggaagc gaggagacat agacaccgca acacagttaa ccaatacttt tacgaatacg
    16141 atttattatg atgcaaatgc ttcagacaat aaatacttta acgaaaatgc gcagaaagct
    16201 gtcaatgcgc ttatttttgc gttattggaa caagcggatc aaacagggga ttactccaaa
    16261 ttaacaccaa ataatctcgt tgaactactt tcagaaattg gcggttttaa ttattatcca
    16321 gatcctgaga atgaattcaa gcagctcaat gccttagatg agttcatgaa tcagttaccc
    16381 cctggaaata tagcaaaaaa acaatatggg gcaacaaaaa ttggatctga taaagcgaaa
    16441 gggaatatct tgtctacggc aattacagga ttgaatccat ttaccctaac gaaaattgcg
    16501 aagatgactt ctcaatcgac cattgattac aagtccattg gttttccgaa gtatttggat
    16561 ctcaaattac atgaaagcct attaaatcaa cgcattgtcg tggaatttta tcataaggga
    16621 aagaaatttc acgaggaacg agtcaaagtt ggctatcgtg gtttttgtga aatcaacttt
    16681 gattgtcatt tagagcaggg agatcatgtt tgcttaaaat acaatcatca aggaaaacaa
    16741 tatgttgctt ggtatgagtt ttctcaaaaa gtattgaaag acgaagaggg aaaaatcgtt
    16801 tatcgaaaaa aacgaggaga aactcatttg ccagaactag aaaaagaagc cgttctaaca
    16861 ttagatgaga agaaaagtaa cctattagta gagtctattc ggatgcacta taccgataaa
    16921 ccaatggcta tttttatgtt aacgcctgat tatgatccat ctaaccatat cattgttagc
    16981 atctttctta gtcagttgta taaagaatta tctacgcaat gtgttaagac taaaggagat
    17041 aaatgtcacc gtaggattca ttttatttta gatgaatttg gaaatatgcc accgatggac
    17101 aacatggaag gaatcatgac ggttacggca ggtcgtaata tgttatggga tctgtttatt
    17161 caatcttatc agcaacttta tgcgaagtat ggagaacaaa acgcagcgat tatcaaagaa
    17221 aattgtcaga cacatattta tattatgtcg acagatgata ataccatcga agaattttct
    17281 aaaaaagtgg ggaataagac ggttgaacaa gagaacagta ccatcaattc attaggtatg
    17341 aatactcata tcaatcgcaa tgttgattca gatcgtatct taactcctga aagaatttct
    17401 acttttttag aaggtgaaac gattgtactc gctcctttaa aacgacaaga tttgaaaggt
    17461 cataaaatac ggccgtatcc tatttttaat catggggaaa cgaatcttcc ttatgcttat
    17521 caatatattg aagatttcaa tccatcgtgg gatatcaatg atatagacat acattgtaca
    17581 catgcggact tggatcttaa ggctttacaa attgattatg attatttcct accagatgaa
    17641 tatgctagaa atctctatca tggactgcat gatcaattag aagaaaaaaa cgaagaaaat
    17701 atgttttata aaatcgttca atcttttgag aatgagacgg ctaaaaaatt gttaattcaa
    17761 ttttacgaag aacaagcaac ggatcgtctt caagaatatg ttgaaaagtt gatcaaaaat
    17821 gaagaaattt ctgaaatgat taggaaacgg ctgttattgg aagctgaaaa ggcggtgaaa
    17881 gaatgatcat tggaggaatt ttctctggca tagccgactg gtggtcaggt ttattcggag
    17941 aaggggatgc agatcccaaa accgtaacga aagtcttaca agattatggt aattatctgc
    18001 agtatcaaga tgtattcagt tggatttgta atactttttt atggggattg atcaaagggt
    18061 tatataatct caatacttta ttagaaaaaa caatttatca aagctttgat ttaaaagact
    18121 tattgaacgc cgctggtgtt aatgaactat ttcaaagttt gattagtaag gtattggctc
    18181 ttttttgtgt cgtcacatta atttatttag gcttgaaatt ttttatgagt aaacacccac
    18241 cgaaagtcaa aaatatcttg gttaatttat ttatggccat gatcttgatt tttggtggta
    18301 gttctttaat cgatgaaggg ttaaatatca gtaaaaactt ttatggagac gtcactgcag
    18361 caaataaagc agataaaacg ggttcacctg cttttcaaat tattcaaaat aatgtgattg
    18421 atgtcagtac attactagcg actgacccga ataaagtaga atccttacca acagataaaa
    18481 gaaatgcttt aacagcgaaa aatttcaaaa cagcgaatat caatgggatt attacacctg
    18541 atcaagctaa gaaaatgtct gaatcaagtg atattagtac agtacaaaaa gatcggttaa
    18601 aggcattaca gtatcgacta gagttagacg ataatgggaa agaaatccct gttcaaatta
    18661 gtgatgaagg tttagcaaaa tatgtgtata cgtcaggcta tcgtcgatac attagtagtc
    18721 cagggattat tttggctggg gaatcttctt tgtcggtagc ctatttattt attttgttta
    18781 caatcgttac ttgcattatt gagctagtat ttaaaaaatt ctatttagta attgccgcgt
    18841 caacggactt tgagacagga caacggatga aaagtgcgat agaagatatt tctcaatcct
    18901 tcttgttatt ggcgtttacg attttagaat tacgaatcta tacattaatg ctttcaggta
    18961 tcggtgattt gcatgcggta ggaaagatca atggattctt gtatgttgtt gctttaattg
    19021 tgctaactat tgcgctattc aaaggctctc aatcggttac gaagattttc ggtgttgata
    19081 cgagtctgaa aaacggcagc aattctcttc ttagcatgtt tgccttagga aatattgcga
    19141 aaaatgtggg tagcggagct aaaaaccttg caagtgctgg taagagtggt ttaagccatt
    19201 taaatgatat tcgtaagaat ggatttggcc atcgaaaaga tattacccat gataacgggg
    19261 aagaacaatc agacgataaa gctacaactg tacctaatag taagaagaca gtttccgaaa
    19321 tgttcgggaa agcgaaaaat ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg
    19381 aacgtgggtt aaaaggaaca gttgaagatc ttacagaaaa agccaaagac accttagaag
    19441 agaaattaga cggcacgaga gctaaagaat ttgctgaagc tgtgaagaat ccatccgaag
    19501 cactagaaaa acaaaaagaa aaagcttcag cagtcaaaga aggactaaaa gatgcgtatg
    19561 gtgaaggaca agtcaatgca gcagtgaagg caaatacaac aagtgaaaaa gaaaatcaag
    19621 aaggtgcagc gattccttca gccaaagatc accaagatga gttacattct aacgaaaatt
    19681 cgatcggaga aaaagattca gaagaaggac aacatggtca gttaccaaaa accgatccag
    19741 aacaatatcg ttataaatct aacattaatt ctgaaaacga aaaacgcatt tcagatttac
    19801 caaaacgaaa tcaagaagta caaacggatg aggattccat cgatctgcca aatgataatg
    19861 gcatcgaagt tggaactgaa aaatcaacga aaattggttt gcctaagaca caaaatttac
    19921 caatgaaaga tagtcgtttg aatcaattca aagagaatga tcaaagcttt gttcatcaat
    19981 cacctgcagg tactgttcaa atgaaatcaa gtcgtttgcc tgaaaatgga aatctgacaa
    20041 ctgtttctta caatttagct gaaagtggtg aaacagagaa tgcgtccgtt caaaatattg
    20101 tgacaaaaga agctgcacct aagccaatca ataaagttca taatggatac agaccagcaa
    20161 acgtagcatt tactgcaccg acaaaaataa atgtgacaag agaaactaaa aacaggaaca
    20221 atgaattgaa agaacaacta ttgaaggata tcaaaccata aaaggcctat ttaagggcct
    20281 ttttctttat aaaacaagtt taaaatggaa aggagaaaat tgtggaggat attttaatag
    20341 ctacgtttat aactgcactg ggagcatttt taacgatatt tgtggtgact attaaaggat
    20401 ttaaagatac ggatagttta caaaaagatc atcagtctat tttagatggt caaaaaacac
    20461 ataccgaaag cattattgta cacctttcag aaggattcac ttatttgaaa gatgctatgc
    20521 ccacattcac agtaaaatta acggaaatat ttcattctgt tgagcaaata tcgtcagaat
    20581 tgaaagagca aagacaacaa aaagatcgtc aacttggctc acttaatttt aggcaagaaa
    20641 cgatacaaga atcattggag aatgtaaatc gattaaacga tgaaatgcaa cgagttcatt
    20701 tggaaaatga agaattacgc gcacaaaatt atgatttaat tcaaaagaaa agaaatctgg
    20761 aacaaaaaat tgttgaatta aaagaagaaa atcgtcaatt gaattatcaa ttacgacgag
    20821 gaaaaaatcg ttctcgtgat ttaggaaatg aattagagtt gtaatattta gaaaaacgct
    20881 gtgagaaatc acagtgtttt ttatttctaa aaatacaaaa tataaggggt atggttatat
    20941 tatggataaa tacattcata tgaaaaatcc tccaagattt agtggggaat ggaagttttt
    21001 cgcctttttc tttttagatc tattaacgct tggtggctgc tttttttggg cgaaaatagt
    21061 caataatctt gtccacttac cactcttttt tgcactcttg aattatggat tatggcttgc
    21121 gatagcgtta gtgattgttt ggcgacccta taataaccct ggtcaacgcc aaatttttgc
    21181 gattatcgaa tctattttct ttacggataa gaaccactat catgcgttag atcctaaccg
    21241 aaattatcat ggtacgtata aggaggatga ataaatgcga gtcaaaggac aaaattcagt
    21301 tgataagaaa tccattttgc agtttggaaa accagaagag cgctctatct tggacttatt
    21361 tccatataaa tcctgtacag aagagggagt cttaattact agagaagatc attttcagcg
    21421 ttattatcgt gtcgtttcca ctgacgtgga gggattaaat gaacaagaga aactagaacg
    21481 aatgaatcag ttgaccaccg tcatgcgtac gtatgtgcca acaattaagt tagttagttt
    21541 gaccacagag acggatctaa cggaacaaat cgtgcaaaag cggcagttat tagataaaaa
    21601 tcgtatggaa cagtcactag gaagaaatct acagcgatta agaaaatatg aaaagaaatt
    21661 agtagaggag atccaggaat taaaacgggc agaacaagaa cgtcctgact taagcttctt
    21721 tttcgttatt gaaggaaaga caatcaagga tttacaaact agaaatcgtc agttaatgcg
    21781 atctagtggc attttagggt taaaaccact ctacaaaaaa gaattgatta agattcttta
    21841 tcgaatgaac aacatgaatg acgaataaaa aagatggagg aaatgattca tgctagatgt
    21901 gataaaaaca gctttttcaa cgcaaagtaa tcaaaaggtg attgatccat taaaacaact
    21961 tggtaatcgt gaaatagaga ttttaaaatc aaaaggttat gatttagatt tcttaaaaca
    22021 agtgatgcca caaggtgggt tgaattttga tgaagaatat gtgacctatg gaaatggtta
    22081 ttcacgatgt gtgacagttt atcattacac aaaaaatcct actctttttt ggttggccaa
    22141 tctcatgaac aatcctcata cagtggccac actagatgtt agcacagatg aaaagcaaaa
    22201 agtgattgat caaatcaacc gcgcattaga tgaattaaat gatcgatcag ataatgaacg
    22261 aaaagcgact gaccgaaata aaagtgcgga agaatacctg gaactagcgg aatatgcccg
    22321 aaaattgact tctaatgggg aaatttctaa acaagtcaaa gtgagagtac atgtttacgg
    22381 aaatacgatc caagaagtgg atgaacgggc gcaggaactg atcgaaaatc taaaagggat
    22441 ggattatcgt gcggtgactt atctgcataa aaccaaacga gattggcaat cgatgtttac
    22501 catgttcgat gaacaagctg aatggtttgg aaatatcaaa gggcaatcac ttccatcgtt
    22561 aaacattggc ggaggattcc cgttccacca ccaaacgcta aaagatccag gaggcacaca
    22621 atatggggaa accatgacag gcggcccatt tgtctggaat gccacgtata aaaatagtgt
    22681 acgtttatct accaatacct tgcttctagg aatgatggga gctggtaaat cgaccgttct
    22741 taaaatgatc gcggaagccc atttagctgc aggagacttt atttggggct ttgaaaaggg
    22801 aaaagacttt attcctttct taaaggaata taacggtatt atggtgcgtt tagacggatc
    22861 tgacgggatg atcaatcctt tagagatctt tgccacacgg acgtatgatg aagcatccag
    22921 tttatatgac gatggttcag tcaaagattt gaagatcaat gaagcagcgt cctatcaaac
    22981 gcacttagac aaagtagtct atcaggtaca gttagtgagt cctcagctaa aaggaacgat
    23041 gaaagcagaa ttcaagacct acttgaatcg tttttatgaa gagtatggaa cagtaccacg
    23101 cggttttact tcctcaaatt cgcgttcaaa tactgatacg caagtcacag ggaaagatcc
    23161 agaagcttat ccgacgttca aagaattttt agccttttta ggacaactgg aactaccagg
    23221 ggcttcccaa gagaaaaaga atcggaaaga agaaatggag tctatcgtgg aatccttatg
    23281 tgagacttat ggcatgattt ttgatggtca tagtacgatt cgacaccttg accaacaaca
    23341 gttggtcttt tttgacattg actctattac aggcctagat cgaggggtac aacaatgtca
    23401 gatgtacatg gccatgacgt tgatttggaa tcaggcattg attcaaggac gaagacaacg
    23461 acgcttaatt aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat
    23521 tgatgagtgc cataatgcga ttaatccaga tttcattgaa acggtaaatt atgtcacaaa
    23581 cttccaacga gaaatgcgta aagtagaagc aatgactgtt ttagctacac aatccccaca
    23641 agaaatggtg ccagaaaata tgtcttctga taacttctcg aaactgaaga aagtctttga
    23701 attttcttct acgaagatct ttatgcgtat ggatgaatct attttacgtc atatagaagg
    23761 attagttggt aaatcaatga cgaactcaga attagaatcc attcctcaac aaaatcaagg
    23821 agacgctgtg atcaattttg gctcaaaaga aaccattcgt gttcatttta gtccgaacaa
    23881 acgtcaatta aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc
    23941 aaacagtatg ttaagcgtcg tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct
    24001 gcaggtttga ttactatatt gatcactgtg tttgtgaccc tttttctaat acttgcggca
    24061 ggttctattg ataattctga ttcaagtagt ggtggcgaag cttttacagg agaatattcg
    24121 gaaggcttac ccatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg
    24181 caatatgctg taggtgcagc agtgaagtat aaattattac caagtgtgat cctttcgcaa
    24241 tacggttatg aatcagcgtt tggtacgtcg gcgtccgcaa gaaatgatct caattatttt
    24301 gggattacgt ggtttgatgg gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc
    24361 attgaaggtg gctggtacat gaagtttccc aactcaaaag ctgccttctc ctattacggc
    24421 tttatggtag ccacacaatc aaattttaat gcttgtgtcg gaaataaaag ccctggcgca
    24481 agcttattga tcttaggacg aggagggtat gccgccgcag gaattactga agacagtgcc
    24541 tattataaaa attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt
    24601 gcgattaaac actggagaga aggcggcgga actggtggta cgattacagg aagctggggc
    24661 aatccattcc caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg
    24721 aaaaatccag gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca
    24781 gtcgatcatc caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc
    24841 aatcctggta tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat
    24901 atggtgtatc aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa
    24961 gtgaaagttg gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact
    25021 cgaatggatt ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat
    25081 ccgttaccat ttttgaatag cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa
    25141 accttaaaaa tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg
    25201 ttttggcagc ttaaagaaga acaagagaca aatacctata aaattgaaga agtggctaaa
    25261 aacgcgtctg ataagctcaa aaaaaattct tctgaagcac aggcgaagat tgacaagtta
    25321 aatcaaacca tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca
    25381 aaagcaaaag aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg
    25441 gccattgata aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc
    25501 caagaagtga tcgacaaatt aagtcctgta gacgatgatc accaacatga tgattacgga
    25561 agttactctt ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag
    25621 gaacaaaatt ttgtcgtatt tgttgattat acaatcggca atcctcaatt caaagatgtc
    25681 aaaccacaaa aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg
    25741 aaagtcacag actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg
    25801 agcaagacaa cgttttaaaa aacgaaaaag ttatcttaga tggctgggga tctttatgat
    25861 tctcggcttt ttgattcttg ctagtccgat ttacacacat aaaaaggtag acataccaga
    25921 tgaagtaagc acgaacgcat ttttaaacac gaaggaactg acgatggttt caaaagaaaa
    25981 aggagcgaac cagcaattag tttttgaatt tgcgatcgac gatgaagatc agagcttatt
    26041 aaaagaacta gctaatctta attatcgtgt agaagtaaag acagctaaag gggattatca
    26101 agcaataaaa gccaaagtga tcaaggtgtc tgatgattat tttgtagtga agttagcaca
    26161 tgtaccagaa gaatatattg ctatgcgatt aactattatt cctgaaaaaa ttgatccaaa
    26221 ggtggatatg caagaaccgc aagatctgat tttttatatt cacgaagata aggtcaaaga
    26281 tcacgtgaaa gacgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa
    26341 gaaagaacaa aaagcagcac aaaaacaaat tgaatccttc caagcaacca ttgatttgaa
    26401 tgaagaatta gtgaagaaac taaaagatca attgccttat caagtcgcag aagatcagga
    26461 aaatactgaa aataagatta atagttacca gcaagaaatt gaaacatcga aaacgcaaat
    26521 gaaagatcaa gaagagagca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga
    26581 aaatatttag aagaataggt ggagtaaaag aaatgaagcg agcgaacttg gtcggcaaag
    26641 atactttcgc tgagcagaaa gtattgatta aagcctatct caaagttgta aaagctaaag
    26701 aaatatgtca agaagtgaat tatctaacac aacaatatct gagttgaaga atggtagaaa
    26761 gagtttagat accttaaaat tcagatcatt tgaagctcta tatatagctg ctaaaaaata
    26821 ttttacagct gataataaat aaaaaattaa ggtgggttct atgtgaaaaa aacgtatcaa
    26881 aagtttttat ggattagcta tatcgtccta tttatcgttg ggtttctcgt cagtgtgtcc
    26941 gtgatcgtcg gaacgctttt tcttttgttc tctgacgaag atctggtact taaggggtta
    27001 atgagtacgt tatcgtgtgt catgttactt tatttatgta tacagatcaa cgaaattatg
    27061 ccccctcatt ggcaagaaaa gaagaaagaa tttaaggaag aaatcgaaat aaaatccaat
    27121 gactggattg aatacacatg gcttcctctg aaaagaaata gcaaacgaaa ggaattttgg
    27181 gcattgcaat tatggccaat aggaaaactt tttatttata tggcttcact tatggttact
    27241 ttaatcggtt atttggtggt ggtctatggg gtggcctatt acttgttaag tgagccaaaa
    27301 cagatcgcgg cgtcaaatat tttcacggca gtaatgacat taatgtgtgt catgtgcttt
    27361 tctattttta gttacttact cgtccaattt atcaggcaaa atttaacaat gagtaaactt
    27421 ttcaaaagtt tattggactt acttatctta ggcagtatgt taacgactct aacagatcgg
    27481 ttcgaacaat ctgaaaaaac ggtagagggg ataaagaact tcttaacgac gattgatcaa
    27541 tttataaatg gatatatgta tttgtttgaa gtaggcttta ttctcacttt gtttggttgt
    27601 gtatttataa aactcattaa aagatcaatc gaaaaagaaa tggttattga tctaattccg
    27661 acgctgagcg gctatgagta ttacgttgat tttggaaagt atgaggagtt gatccatacg
    27721 gatatcgttc acacgagtat ccatgaagta accgttaagg gcatcagatc tactttaaaa
    27781 gatggacagg agacgaaaac aatcgaccga agaaaagagg tctacaattt ttataaagtt
    27841 gaagcaagtc tacgatatgg cattgggaaa ttgaggttag atgggagtaa tattcaacaa
    27901 aacaaaccat atgttttagg aaaagtggtg atttagaact tcctatgaaa gatgaataaa
    27961 taaggagaat cttttgaaaa tgattcttct ttacagaatt gtgcttatag ttggcacgga
    28021 gttaaaaatc ttaaagaagg agcagttatt caaagtgaaa gaacatcata atcagttact
    28081 atgtattaaa acgggtacga tgaacattca ataccgaaca gaatatttag aattaacgaa
    28141 tcaaactgat tttttgtcga ttcgagagca acataagcaa gaaaaattaa tggctactta
    28201 tagccaattg attgatgagt gggaacaatt caaggaatct aaaacataaa attccattta
    28261 aacaccttat tttttaaaga gcataaggaa acgtgtaccc actttcaaaa taaagtgggt
    28321 agataggctg tgcctatcac gtttctgaaa aaaataaatg accccaaaat tttcaattta
    28381 tgtgtaggtt tatgggtagc aaaaatatga atgtgggcag caaaaagttc atttgtgggt
    28441 agtaaaattc aaatgtgtgt agcaatgtgt gtagctacac acattgctac acacgctata
    28501 acatatagtg aaaaggagat atttatggaa gtaaaaataa gagatattga tgaaaaaacg
    28561 attcaaagaa ttgaccgaag atgtattgaa ttaagtgaac gcaccgggac taaatggagc
    28621 cgaaatcgat acctaaaagt tttgattgaa aatgattttg accatgaact tatggatcat
    28681 agaaaagatc aatttgatcg gttgttagaa aaatttgtgg atattcaaac ttataatacg
    28741 aaaattttag aagaatatat tgcaacaaat aatcaattaa tcgccttgtt aattgaataa
    28801 taaaaatagt attgtgaatt tataaaagga ggaaatcaat tttggcaaaa gcaacaagaa
    28861 aacatgtata tctttacgat atgcatatcg aagtacttga tgaaataatt tctactcatg
    28921 aagatatatc taattatagt gaagcagtca gacatttgtg tatgatgtat gatccaaaaa
    28981 tcgaagaaaa aataaatcca attcaaatta aattgaatgc aatgggaaaa gaaatttcta
    29041 tattaaccga aatggtttca gagtttgcgg atcttcattt gaaagataca ggaatactaa
    29101 ttggtacaaa ttcaactgtc tatcaagaag caaaagagcg tgtggaaggg aaaataaaag
    29161 cgaatcaaac gaaaaagtat tctcctaaga tcaagaaacc aataaatgaa tctgttcatg
    29221 ccagagtaga cccgtttgat ccaacaaagt ttttaaaaag ataaaaagta gaaggggaac
    29281 atgatgaata aatcgcctgc agtcattctc acttgtcaat ttaccttgcc caataaaaaa
    29341 tctttttcaa catacattga ttatatgaca agagaaaaag cgctggaaga aaaggaacga
    29401 cgcacgccgg acgaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat
    29461 attcctgaag gagaaacttt ttcggagagt aagacagata aagaattcag tgaaacgtcg
    29521 aatgaagctc aatcgttgat ggaacatgga atcaattttg acaagttaga ggaacaagat
    29581 tttaccaaat atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg
    29641 acgcaaaggg aacaaaaaga ataccaacgg ttgaaccaag caatgaaaaa atatcaaaca
    29701 gagccacata aaaaagcttc aggaaatctt cctggagttt tttctatggc ttcagacgaa
    29761 gtgaaaggaa gtgatctgaa ggagatcaaa caaatcttcc aaagagggca acaaaaaggc
    29821 tcaatcattt atcaagatgt tgtctctcac gacaattcgt atcttgaaaa aatgggcttg
    29881 tacgatccca aaacagatac cttagatgaa gaaggattaa aagcagctgg aagaaaaatg
    29941 atggagacat tattcgagaa agaacaattg aatgaaacag gttactggat ggcaacgatt
    30001 caccgtaaca ctaagcatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt
    30061 aaaccttaca cggaggttga aaatggtgtg agttatatcg taccgaaagg taagagaaga
    30121 caagcaacat tagacgcaat gaagaccaat tatgtacatg agttagaacg tttttcttat
    30181 gaaaaggatc gaatgattgg tatggagaaa cgaaatttat tgacgcggaa aagtgatttg
    30241 cgtaacacct taacaaaagt ggtaaaagag ccagcgcgtt acgatcgaca cgcaatgaac
    30301 ttattaaatg aagtatataa aaccctacct gacaaacgtt cggaatggca ttatggggat
    30361 gaaaaacgaa ccaaactttc accatacaca agagagaaac tcgatgagtt aaccaaatat
    30421 gtgttaacaa aagaacctga atatcaagaa tatatacaga tttcaaaatc attgaaagaa
    30481 gaagcaaaaa gtctttatgg ggaaagcaag agagaatcaa aggatgcaga aaaaaatgct
    30541 ctctttgatt tgaagaagcg cacaggaaat gccattctaa cagaactgaa gaaacaggat
    30601 agtcaaataa aacctttaat agaaagtatg gatcagatgc aatctattta caatgaacga
    30661 tcctttcgaa cgcttaaaca gttaccaacg aatgaataca aatatagtta cgaagaattc
    30721 atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc aacggagttt gcgtcaatta
    30781 aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa gtaaagccta cgaagagcaa
    30841 caacgaagaa ttcaaatgga acaagaaaga cagtaaagac agtaggagga atagcaatga
    30901 aacaatattt agatcaatgg aaagttatag aaggatcatt aagagaagaa cgaatcgaac
    30961 aacttcccga ttgtttagaa aaagagcatt tgtttcagat aagagaaatg ttgagaaacg
    31021 aacagtttga tccaaatcaa tttttagtcg ttgagtactc agctactggt gtgtactgtt
    31081 gtaatcatgt gaagggtgaa aaatatttta tcattcaaga atatgagggc aagttagcgc
    31141 cttattatac tacttgggaa atgaatgaag aggggatcaa taacttcccg tgtaaaagca
    31201 ttgaagagtc tatttcactc acagaatgtt agttgttaag tgaataaaag aagtaaaaga
    31261 gtcctgaacg taattgtcca ggactctttt tttcaatgaa ttgttttccg ccaattcaat
    31321 gagtggtgcg atttgcgact acattatatc aaaagaaaga gaagggagga agaagaaatg
    31381 gcttattcaa cgattggagt tgttgttgaa aaaagcagag acaatctcgt ttttgtgaca
    31441 gaaattcaaa cgggcagagc ttttgtcgtc acagacaaag cggcaaaagc gtatcaaagt
    31501 ggcgatattt taacgttaaa tctgactacg aagacgttcg tcgatgcggc agaagattat
    31561 cc
//
LOCUS       DACEZN010000015        31560 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEZN010000015 DACEZN010000000
VERSION     DACEZN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.015"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..459
                     /locus_tag="IXH60_000539"
     CDS             <1..459
                     /locus_tag="IXH60_000539"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Enables the production of acetyl-CoA by
                     phosphorylating acetate in the presence of ATP and a
                     divalent cation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1989505.1"
                     /translation="DIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGLTGLSSDMRDL
                     EKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDGHVRSEIIKGM
                     SWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERLRG"
     gene            complement(896..2479)
                     /locus_tag="IXH60_000540"
     CDS             complement(896..2479)
                     /locus_tag="IXH60_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1989506.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(3057..3200)
                     /locus_tag="IXH60_000541"
     CDS             complement(3057..3200)
                     /locus_tag="IXH60_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1989507.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(3423..3902)
                     /locus_tag="IXH60_000542"
     CDS             complement(3423..3902)
                     /locus_tag="IXH60_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1989508.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3975..4259)
                     /locus_tag="IXH60_000543"
     CDS             complement(3975..4259)
                     /locus_tag="IXH60_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989509.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(4337..4531)
                     /gene="ssrS"
                     /locus_tag="IXH60_000544"
     ncRNA           complement(4337..4531)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IXH60_000544"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4603..5889)
                     /locus_tag="IXH60_000545"
     CDS             complement(4603..5889)
                     /locus_tag="IXH60_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1989510.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            6027..6698
                     /locus_tag="IXH60_000546"
     CDS             6027..6698
                     /locus_tag="IXH60_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1989511.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6664..7152
                     /locus_tag="IXH60_000547"
     CDS             6664..7152
                     /locus_tag="IXH60_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1989512.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            7165..8112
                     /gene="prmA"
                     /locus_tag="IXH60_000548"
     CDS             7165..8112
                     /gene="prmA"
                     /locus_tag="IXH60_000548"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1989513.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            8113..8877
                     /locus_tag="IXH60_000549"
     CDS             8113..8877
                     /locus_tag="IXH60_000549"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1989514.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            9110..11323
                     /locus_tag="IXH60_000550"
     CDS             9110..11323
                     /locus_tag="IXH60_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1989515.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            11334..11780
                     /locus_tag="IXH60_000551"
     CDS             11334..11780
                     /locus_tag="IXH60_000551"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1989516.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11951..13210
                     /locus_tag="IXH60_000552"
     CDS             11951..13210
                     /locus_tag="IXH60_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1989517.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(13299..14246)
                     /locus_tag="IXH60_000553"
     CDS             complement(13299..14246)
                     /locus_tag="IXH60_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1989518.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            14640..15932
                     /locus_tag="IXH60_000554"
     CDS             14640..15932
                     /locus_tag="IXH60_000554"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1989519.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15947..17716
                     /gene="aspS"
                     /locus_tag="IXH60_000555"
     CDS             15947..17716
                     /gene="aspS"
                     /locus_tag="IXH60_000555"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1989520.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17825..18700
                     /locus_tag="IXH60_000556"
     CDS             17825..18700
                     /locus_tag="IXH60_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1989521.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18966..20849
                     /locus_tag="IXH60_000557"
     CDS             18966..20849
                     /locus_tag="IXH60_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1989522.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20924..21208)
                     /locus_tag="IXH60_000558"
     CDS             complement(20924..21208)
                     /locus_tag="IXH60_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1989523.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(21198..21932)
                     /locus_tag="IXH60_000559"
     CDS             complement(21198..21932)
                     /locus_tag="IXH60_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1989524.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            22061..22684
                     /locus_tag="IXH60_000560"
     CDS             22061..22684
                     /locus_tag="IXH60_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1989525.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            22876..23997
                     /locus_tag="IXH60_000561"
     CDS             22876..23997
                     /locus_tag="IXH60_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1989526.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            24004..27132
                     /locus_tag="IXH60_000562"
     CDS             24004..27132
                     /locus_tag="IXH60_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1989527.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            27151..30345
                     /locus_tag="IXH60_000563"
     CDS             27151..30345
                     /locus_tag="IXH60_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1989528.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(30491..31198)
                     /locus_tag="IXH60_000564"
     CDS             complement(30491..31198)
                     /locus_tag="IXH60_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1989529.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10289 a         5705 c         6696 g         8870 t
ORIGIN      
        1 gatatcgatc cagctgttct cccatattta atggaaaaat tagagattga tattgatgaa
       61 atgatcaatg ttttgaacaa gaaatctggt ctgctaggcc taacaggatt gtctagtgat
      121 atgcgtgatc ttgaaaagaa ctatgaaaaa gaacatattc gtttagctta tgacattttc
      181 gtggaccgca tccgaaaata catcggcggt tatgtgacaa cgatgaatgg tgttgatgca
      241 attgtcttta ctgctggtat cggcgagaat gacggacatg tacgtagtga aatcatcaaa
      301 ggtatgtctt ggtttggttg tgaaatcgat ccagcgttga atgaattgcg tggtgaagaa
      361 gtagatatct ctacaaaaga atcaaaagta agagtattag tgatcccaac agatgaggaa
      421 ctaatgattg cccgtgatgt ggagcgtctg cgcggctaag ccaaagcatt cgttaggata
      481 aaagatgaaa cgcatgcaag agggaattct tgcatgtgtt ttttttgcgg agaagaccgc
      541 aaaatggtaa tttaacttca atttcccttc ttttttacag gatcttctta gggaattttg
      601 cttggaaaag tggtcaaaag ggaaaagact ggcattttat ttattgattt tcaggaaaat
      661 cccaatattt tattttcatt catctttata agggacatta catatgtaaa aagaaaaaac
      721 atttttagat cagcctcgtg ggctgtcgac tttattcttc gctttatgtt tagctagaaa
      781 accgatccga aacattcatt gatcgaataa taaaaacttg attttccacc tgtactttgc
      841 ctgaatcgtg aaagcacagg tatgaaaatc aagttttttg ttgcgaaaaa acgtcctagt
      901 caatcagttg gccgagcggc tggcggctga tctgctcctg atcaataacg agtgcttctt
      961 ggaagtgttg ctgcaaaaag tagcggtcat ggctgactgc taggagcgtt ccttgatagt
     1021 cttgcagcat ttcttcaatt tcttctcgag catagctgtc gaggtgattg gttggttcat
     1081 ccaaaatcag gacgttgatt ttttctaaaa agagtttcag caacgccaaa cggatttttt
     1141 caccaccgga taaatctttg atccgtttac ccacatcgct ttgataaaag ccacttttgg
     1201 ctaattgccg tcgagcgact tcttcgtttc cagtcaatgt gatggcgtaa gccagcaaac
     1261 ggctatcggg atcggcaaac tgcagctgct gcggcaaata accgatttgc aagctactac
     1321 ctaatttgat ggtgccaccg taaggctttt cctcaccgag taataatttg agtaaggttg
     1381 attttcctgc accattgtcg ccgatgatgg ctaaatgatc tccacgatag actgcaaaac
     1441 tgctgtcttt gaatagtact tgatcagcgt actgctggtg caagttttga atgatgaaaa
     1501 cttctttgcc agattttcca ccattagaca agctttgcag ccgatttttc gggggaggtg
     1561 gcggtttgac gagtgtcagt ttcgccaggc ggcgttccag ttctttggct tttttgaaga
     1621 aggactcatt gtctccttca tgtgcccact gacgaaaacg acgaatcatg actttcaacc
     1681 gctggacttc tttttgctgc agttcgtaat tttttgtcag ctcagccaag cgagcttctt
     1741 tcaagactcg aaaacggctg tagtttccgg gatactcaat caatgcacct tcttccagtt
     1801 cccatgtttt tgtggtgact tgatcgagaa attgccgatc atgagaaacg accagaaccg
     1861 atttttttgt attctgtaaa tagttttcca accagcgaat actgttaaga tctaaatgat
     1921 tggtcggttc atcgagtaaa agtaaatcgg catcggctgc taaaattttg gctaaagcga
     1981 ctttaactcg ttcgcctccg ctaagttctg ttaaccgtgc ttcctgacgg tgtggaatcc
     2041 ctaagccttg caagatcgct gcaatccggt cttctaatac gtaaccgcct agttcttcaa
     2101 agcgatgctg cagctcaccg taacgggcca gacacttctc taagtttgta tctggttctg
     2161 tcatcaatgc ttcgtagcga ctcatttgct gtttgaggtc ctgtatctca gcggcagaat
     2221 ggagcaagta gtggttgacg gtcgcttcgg aaactgtaag ttcttgttcg accgtcccga
     2281 tctttaagcc ttttttgcga ctgataacac cgctgtcgac accttctctg ccggtcaaga
     2341 tttgtaaaaa ggttgatttg ccagttccgt tgataccgac taaggcaatt ttttcaccag
     2401 cttcaatttg acaggataaa tcctcaaata atggattccc gccgattgtt tttttaatag
     2461 aaattgcttg tatgatcatg atctctctcc ttgagaccac tcaaaaaggc catggttatc
     2521 catggcctgt gttatttaaa ctcagattat catgagaaaa ataccttgag atggccattg
     2581 attttacgtg aattcaactg ttttggacaa acttgtcccg tcaacagcaa ctgtaaaacc
     2641 aattgcatgg ctaaatgcga tatggtatcg ttggtaattt tcattacttg ttattttagt
     2701 catgcgcatc atagtatttt ccctccttcg tgtagttgtc ttttccagtg tacctgaaag
     2761 tgtcagaaag ttcaactagc gctgacattt acttaatatt tatcgatgtg taattctgtc
     2821 agagagcttt ttttatctga ctgagggtaa tgtctcgtgg accacatcat tgtattctga
     2881 gctaaaccag cgttcaggcg aaatcatatg gtgtttgata aaaaactttg tctacatgac
     2941 ttccttttgc catttgctta ataccgttgc attaaacagc tcttctcttt ggaaatctca
     3001 ccaacatcgc gttcaggagt atcgttgtgg ttgcaatggc aagcggtagc ctttcatcaa
     3061 ttcttgaatg atttaccgaa tgtagcactt gccttctcgc ggtacccgaa cccgaaataa
     3121 aattttagtt tggcgataaa atcggtatcc gccatcgata cttcatattt tttcgtatca
     3181 gattcatcgg aaacttccac attgacgaac caataagctt tggcatgctt aggtcgtttt
     3241 aaaccacacc agggcgacta atcgccctgg tgttcacttt tttttatttt acttgaactc
     3301 attgactgtt ttccctttat tttaaacatt ataaagaccg atgagtgctt gccgcgtctt
     3361 ttgtttttat cacggttttt tgtgcaaaaa aacctgccga tagttggcag gtcgtctacg
     3421 tgttaagctt tggtgtggtc tttagctggc aattggtttt ctaaatcagt tcggacaaca
     3481 agcacatcgc aagctgcgtt tctaataaca tattcagaaa cagaaccaat aaataaacgt
     3541 tctacagcat tcaatcctgt ggctcctaac ataatcaaat ctacttgatt atcttctggg
     3601 atttctctgg caataatttg ttttggtgag ccatattcta cgacagaggt tacattattc
     3661 aagccagctt tcttcgcatt gctttcataa tctgccaatg tttgctttgc catttcagta
     3721 gcttgttctg caagcattcc atcaaaggat gaaacggttt ggaaagaacg tgtgtcaatg
     3781 acatgtgcta atagaagttc tccgttattt cggatagcta cattgaccgc ctttttaaaa
     3841 gctaattcag cttcatcaga accatctacg gcaaccatga tttttttgta ttgttgtaac
     3901 ataagcattc actcctcaag tcaatcgtat tgtactcttt tattgtagaa cactttaggg
     3961 aaaaagagaa ttatttactg ctttggttca gcaatttttt ttgcaaatcg gatactgaag
     4021 gtggcagaaa caataattac gatcagtaaa aagaccaagg caataaagcg ttgattgaaa
     4081 aaagctgtta aaatacccat tacgcctaaa acagcatatg cagctccgta aaattgtagg
     4141 aatccttgat ttttatcatt tttgttgatc aatacaaaaa agatatcctg tttattaaat
     4201 aaataatagc taacgaataa taatactaaa gtggcgataa tcattaaaat cctaatcatg
     4261 aacaaatcct ccaaaataag caacaaatag aaaaagcaat ttgccaagag caaattgctt
     4321 tattctttaa aagatggtaa tctccgttaa tgccgttgtt tgtcctgtag tattgatccc
     4381 gcgaattcaa gcaggtgggt cccatggtcg tagcttcgaa ctaccaaatg ataaggcatg
     4441 ttaccagcag tgacacaaga cgggtcccaa gatatcaata aaaatgttcg gtcaacacac
     4501 aaagaggaca attcgaacat cacagatttc ccacctctat agtagcatat gaatttaaaa
     4561 agagcaatga gagagctcgc agaaacagaa ccgttttctt gtctagtttt ttggaggatg
     4621 ctttttccac tcttttatac ggtaatactg ttgacccaga gcctgttcat atttccctgt
     4681 atcttttggc tgataatact gagcattctt cagtttatct ggtaaatatt gctgattgac
     4741 ccaagcctga tcaaaatgat gaggatactg ataacccacg ccacggttga gtgatttcgc
     4801 acctttgtaa tggctatctc tcaaatgatc aggaacatct ccagctttgc cctctcgaat
     4861 atcagcaagt gctgcatcta atgccatgta ggcagaattg gattttggcg acaagcaaag
     4921 atcgacgacg acgtctgcta gtgggatacg tgcctcaggc aatcccaatt tttcagcagc
     4981 taatacagcg ttcactgtac gagccgcagc agcaggatta cctaaaccta tatcttcata
     5041 accgataacc atcagacgcc ggcaaatact tgccagatca cctgcctcaa ctaaacgggc
     5101 gagataatga agtgcagcat ccacatcact tccacgaatc gatttttgaa aagccgaaat
     5161 aacgtcataa tgagcatctc catttttgtc atgggtaaga gcttttcttt ggacacattc
     5221 ttcaatgatc gacagtgtaa gatgaatcct tccttcttta tctgaaggag tcgatagtgt
     5281 agccagttcc agcccattca atgcactccg caaatcgccg ttcgttgcac gcgaaaggtg
     5341 aagaagagca tcttcgtcta actggattgc ttgttgtcca aggccgcgtt ctttatccct
     5401 taaagcatgt tcgactgcca actggatgtc ttgttctgtt aatggtttga cttcaaagat
     5461 ctgtgtacgg ctgcggatcg ctggattgat ggtaatataa ggattctcag tagtcgctcc
     5521 gatcaagatg attcggccac tttctaaatg aggaagcaga aaatcctgtt ttgttttatc
     5581 taatcggtgt acttcatcca ataagaggat cactgtacca ctcatttttg cttcttccgc
     5641 tacaacttgt agatcctttt ttgaatctgt agcggcgttt agcatgcgaa atgcataatt
     5701 agtagagccg gcgatcgcac ttgcgatact cgtttttcca gtaccaggag gtccatatag
     5761 aatcatggaa gagagcatgc gagcatcgac catgcggcgt atgatttttc cttccccgac
     5821 tagatgctgc tgaccaacga cttcatcgat cgttcgaggt ctcatgcgat aagccaatgg
     5881 ctgttgcata ggattttcct ccttcttgtt tttacgtccc ttaattatat catatttagc
     5941 aagatgccta gaaagaacag ccttctgttg ttttgttttc atagcttatc caactaatat
     6001 tgtgtatgat aaagtaggtg agaaaaatga cggataaaca aaagatagat gaattttttt
     6061 ggaataaaac gacgccagaa gtggcacggg atttattggg gatgtatttg gagcatgaca
     6121 caccagaagg aagattagct ggatatattg tagacgcgga agcataccta ggtccagaag
     6181 acgaggccgc ccatagctat ggtttaagaa gaacaccgcg tgtgcgggca atgtatgaaa
     6241 aaccaggtac gatctacctt tacacgatgc atactcatcg gattttgaac atcattactc
     6301 agccagaagg gattcctcaa ggagtaatga tacgtgcaat cgaacccgcc gcaatgattg
     6361 atcaaatgag taaaaatcgt ggcggaaaaa ctggtccaga tatcagcaac ggtccgggaa
     6421 aattagtgga agcattagca attcctcaag aactttatgg tcagtctata gcagatagtt
     6481 cgttacgctt agtttttgag aaaaaaaaga caccaaaaaa gatcaatgct ttgcctagaa
     6541 tcggtatccc aaacaaaggt gtatggacag aaaagccgtt gcgttttgtt gtgtcgggaa
     6601 atccttatat ctctttacaa agaaaaaacc aaatcgagaa agattggggt tggaggaaag
     6661 aaaatgaaaa agaaggatct attaacatat ttagatgaaa taattgaaaa acaagtgaca
     6721 gactatgatg tagctttaga ttgggataca aaaaatcata cgatcgagat cgttgttcgc
     6781 ttgtttgcag aaaataaagc acaatttgag attgatgatg cagaagggat cgtttcgcaa
     6841 gaaccaatta tcgaatttga agacggcgta cttcttttta atcctcagaa atctgttttt
     6901 gatgaacgca attatctagc agtaatccct tatgaaggaa aaaaagggtt ggcaaaatca
     6961 gtagctgatt ctttagtgga atatctaaat gaagtattag ctcaagggca aagcgatttg
     7021 cttgattttt tggatgaaga cgatgacgaa gctgtattcg agcttcactg ggataatcag
     7081 caactagcag aattagtaga agtaaaacag caaaatgaag cagatactta tttaccgtac
     7141 ccaagttatt aaggagtgac agatatgaaa tggaatgaag taaaagtaga aactgaaagt
     7201 gaagcagtcg aagcagttgc taatattttg atggaggcag gggcgagtgg tgtcgcgatt
     7261 gaagattctt tagatgttga acattttcag tcggatcaat atggtgagat tttagataaa
     7321 aatgatttta catcgctgaa agaaggcgca ctagtcatgg cttattttcc ggaaacgact
     7381 ttcttaccgg aaatcttgcc ttttatcaaa gatcgtatca cacaattacc tgagtttggt
     7441 ctagctattg gaaaaaataa agtaacagtt agtgaagtag aggaaagcaa ctgggcaaca
     7501 gcctggaaga aatactatca tccggtgcgt attacgcgaa tgctgacggt tgcgccaagc
     7561 tgggaaaact accagacaga agatcctcta gaaaaaatta tcacactaga tcctggtatg
     7621 gcatttggca caggcactca tcccacgact tccttgacat tgacagcact agaaacagtc
     7681 ttgcgtggtg gtgaaacatt gctagatgtg ggaactgggt cgggcgtgct aagtattgca
     7741 gcaaaatatc ttggtgcaaa agatgtctat gcctttgatt tagacgaagt agccgttcgt
     7801 tcggcgaaag aaaatatgga tatgaatgaa atagccaaag atgtccatgt atcagctaac
     7861 gatcttttga caggaatcga gattgaaagt gatgtaatcg ttgcgaacat tttagcagat
     7921 atcatcttat tgatgatccc agatgcttgg cgtttactga agcaaacagg cacgctgatc
     7981 gtttcaggaa tcattgaaga gaagaagcaa atggttctgg atgccatgtt agaacaaggg
     8041 tttatcgtgg atcaaatttt ccagcaaaaa gactggtatg caattatttt gaaaaaacca
     8101 gaggtggaat agatgcagcg gtatttttta aaagaatcat atgaatcaaa agactatttt
     8161 gttttagaag gggaaaacta tcaccacatt gtacgggtta tgaggatgga acccgaggat
     8221 gaagtttttt tggcattcaa tgatcaaata gccattgttg cagaaatcac ggagattact
     8281 gaagaggctg tttatctgaa agaaattaaa aaagaagaaa atcaaaagga actgccaagt
     8341 tcgattacca tagcgagtgg ctatcctaaa ggggaaaagc tggattggat cgttcagaaa
     8401 tccactgaat taggagcgca agcttttctt ggtttcccgg catcagcctc tgtcgttaaa
     8461 tgggatgcaa aaaaacgtat gaaacgacaa caacggcttg aaaagattgc tcaagaggca
     8521 gcggaacaat cccatcggca gatcgtgcca tcagtagagt tattggaaac gaaacaagat
     8581 ttatttgagc ggctagatga ttatgacgtg gtcttggttg cttatgagga atcagcaaag
     8641 caaggcgaaa aatcaaatgc agccaaaatt cttagtgaaa caaaaccaga tagtcgaatc
     8701 ttagctgtct ttggaccaga aggtggatta tcccgcgatg aagtcgatta ttttgtagaa
     8761 cacaaaggga tactttgtgg attaggacca agaatccttc gtacagaaac agcacctctt
     8821 tatttattaa gtgctgctag ctatcaatgg gaattaatga atccttcttt cagctaacgt
     8881 tcttcattaa taaaagagta tcgacagaaa gaattgaaaa aatagtcaac aatttgtata
     8941 atagaaacaa ttatttatac aatggatacg aaaaatcgtt tgaaaaagct tttaaaaagc
     9001 gaacatttcc gtaaaaaaac gtataactgc acggattttg ggacaattat caaacagata
     9061 tttgtcccga ttcccgtttt accgaaaaaa gaaaggggga tcgattcctg tgacaaaaga
     9121 agaaatttta acgggacccg gtgtcataaa gctcgttagt caatacatgg gtcccgaaca
     9181 tgtagcattt gttgagaaag catgcgaata cgcgactgct gcacatgacg gacagtttag
     9241 aaaatcaggc gaaccctaca ttattcatcc tatccaagtc gcaggtatat tagcagattt
     9301 aaaaatggat ccccatacag tggctacagg cttcttacat gatgttgttg aagatacaga
     9361 aatcacttta gaagatctga gagaagaatt tggcgatgac gttgctatgt tagtggacgg
     9421 cgtaaccaaa ttagggaaaa taaaatataa atcccacgaa gagcagctag cagaaaacca
     9481 ccgaaagatg ctgctagcaa tggctcaaga tttacgagtt atcatggtca aattagctga
     9541 tagactacat aacatgcgta cgttgaagca cctgcgagaa gataagcaaa gaaggatcgc
     9601 tcaggaaact ttagaaatct atgcgcctct tgcacatcgt ctagggatca gccggatcaa
     9661 atgggaatta gaagatacag cacttcgtta tctaaatcca aaacagtatt accgtatcgt
     9721 ccacttgatg cagacgaaga gagaagaacg tgaaaaatac gtaagtggta ctgttgaaga
     9781 tattcgaata gcgacagaag agctggggat ttttgcagaa atctatggac ggccaaaaca
     9841 catttattca atctatcgta aaatgaaaga tcagaaaaag cagttcaacg aaatttatga
     9901 cttgctagct attcgagtga tcgtagattc aatcaaggat tgttatgctg tattaggagc
     9961 aatccacaca aaatggaaac caatgcccgg cagatttaag gattatattg ccatgccaaa
    10021 agctaatatg taccaatctt tgcatactac cgtgatcggt ccggcaggaa atccggtaga
    10081 aattcaaatc cgaacacagg aaatgcatga aatcgctgaa ttcggggttg ctgcacactg
    10141 ggcctataaa gaaggaaaaa atgaaaaagt agaaccagat ggtatgacga aacaattaag
    10201 ctggttccat gagatactcg aacttcaaga cgaaagctat gatgcttctg aatttatgga
    10261 aggcgtaaaa ggagatatct ttagtgataa agtctacgtc ttcacaccaa aaggagacgt
    10321 tactgagtta ccaaaaggat ccggaccatt ggactttgca tacagtatcc atacagatat
    10381 cggtaacaaa accactggtg caaaagtaaa tggcaaaatg gtgcagcttg attacaaatt
    10441 gaaaaacgga gatatcattg agatcatgac ttctccaaat tcatttggcc caagtcgcga
    10501 ctggttgaaa ttagttgcta ctagcaaagc aagaaataag atcaaacgtt tcttcaaagc
    10561 ccaagatcga gaagaaaatg tgatcaaagg ccacgaatcc gtggtcaaat gtattacaga
    10621 tctaggattt acgcctaaag atattttgac gaagaacaaa ctgcaagaag cactcgatcg
    10681 ttttaattat caaacagaag atgatctcta tgcagctgta gggtatggag aagttagccc
    10741 cttgacgatg gccaatcgtc tgactgaaaa agaacgtaaa gaacaaaaaa tcgagcagca
    10801 aaagcaagaa gcagaagaaa tcatgaatca gccgaaaaaa gaacctgaca aaatgaaagt
    10861 acgtcatgaa ggtggcgttg tcattcaagg ggtagaaaac ttactaattc gtatcagtcg
    10921 ctgctgtaat ccgattcctg gtgatgatat cgttggttat atcactaaag gcagagggat
    10981 atccattcat cgtcgagatt gtccgaatgt tcagcctgac aaaccaaatg tagcagaacg
    11041 tttgattgaa gtcgaatggg aagatacatc gaatacacga aaagagtatg atgcagattt
    11101 ggaaatttac ggctataatc gttcaggctt attgaatgat gtacttcaaa cagtcaatgc
    11161 gctaacgaaa aatctcaaca gcgttgaagc acggacgaat aaagataaaa tggcgacgat
    11221 ccatttgacg gttggtatcc agaatttatc ccacctaaag agtatcgtgg ataaaatcaa
    11281 agcagtacct gatgtctaca gtgtacgccg gacgaatgga taggaggaca gatatgagag
    11341 cagttgttca acgggtaagt aaagctgctg tgtcagtcga tcagcaagaa attggaaaaa
    11401 ttggctacgg cctacttgtt ttattaggca ttcatgaaac cgatacgcaa aaagatgtgg
    11461 actatctggt aaaaaaaatt gcacaaatgc gtatttttga agatgaacaa ggaaaaatga
    11521 atattagtgt agaagatgtg ggaggacaaa ttttaagtat ctctcaattc acactgtttg
    11581 cggatacgaa aaaaggaaat cgaccaagct ttgtggaagc cgcacgacca gaaacagcta
    11641 ttcctttata cgaatcattc aatgaaggaa tcagaagtcg cggcatcaca gtcgctaccg
    11701 gacaattcgg agcagatatg tcggtttctc tcgtcaatga tggacctgta acgatcatta
    11761 ttgattctaa aaacaaataa agtaatctgg ttttcactag taaaaactat gaaacacgtc
    11821 gcttaagtta gactgataaa ctctaactta agtggctttt ttgtctgcaa aaagcgcttc
    11881 ttttgtcagc agtcggaaaa aaagttaagc ttaaattata caagttattt tttataggag
    11941 gttgctgaag atgaaagaag taccagtagt agatatcaaa atcacagatt taaaaaaatt
    12001 gtatcaaaaa ggatacaaca tgttggagga gttacgccat gaagcggacg ctcccgtcgt
    12061 caaagcaaag attttcaata aagaagctat cacgatctac ggatcatcag cagcaaaagt
    12121 attttatgat ccaaggaatt tcaaacgaaa aggtgctatg ccaaaattag tattgaagac
    12181 actttttggt caaggtggag tacagacttt ggatggagct gctcatcacc atcggaaaaa
    12241 tatttttatg gatctaatga caccggaaag aatggaagat tatcatcgta tcttagataa
    12301 gaatctgaca caagcacttg aagcacagca tggtcaattt gaactatttg atttaagtaa
    12361 gatggttttc tttacatcta tttgcgagtg ggcaggtatc aatttatcag cgatttctaa
    12421 agatgaggtc gaaaaattag cagaatatca aatctctatg atcagcggaa cgtttacttc
    12481 tccgattgat cacataaaag gtgtcgaaaa tcgcaaaaaa tcagaaaaat gggcgcaagg
    12541 cttgattgaa gaagctcgac aaaatcctgt ggctggaaaa gaaaatgtgg cattatatgc
    12601 ttttgccaat gccacagatt tagatggtca gcttttacct ctggaagtgg cagctgttga
    12661 attattgaac atcatccgtc caacggttgc tctaactgtg tgggcggcat tgatgggaca
    12721 tgcacttttc agtagaccag atttgtatca gcaattaaaa aatgactttt cgacgttaca
    12781 agatccgttt attcaagaaa tgagacgcta ttatcctttc ttcccaatgc tacctgcgat
    12841 ttcgctgaaa gaagtagaag tagatggtta tcgtatacca gaaggcagtt gggttatttt
    12901 agatttatat ggaacagacc atgatgaacg aacagttgaa gcgccagatt cctttatgat
    12961 caaacggtat gtagggaaag caaaagatat ttcctataaa gaagaatatg agatgattgc
    13021 acaaggcggg ggaaattttc gacaaatgca tcgctgtgca ggagaatgga tcactcttca
    13081 tagtctacgt gtgttttccg atcagctagt aaataagttt gagttctctg tacctgaaca
    13141 agattggacg attccattca atcagtttcc gacttatccg aatagccgag cgctacttta
    13201 caagaattag attagcataa acttatcgaa aaaaaggaag atcaaacagt gctgatcttc
    13261 cttttttcat attactataa attgataagg tcaattgatc aaaatccacg aaatgcttca
    13321 tcaatggttt gataatcttc attggataaa tgcaaatcta aagcttggac attgtcagat
    13381 acttgctctg gacgcttagc tccaggtata actacagaca ctcgtggatt cttcatgtac
    13441 caagctaaaa caagttgcgc aggagtggct tggtatcttt tagcaatggg gcgaagtaca
    13501 tcgacagcgg ttaaaatttc tctgaaacgt tctccttgga aatctggatt tctttttcga
    13561 ggatctcctt caccaaattg tttttcctca ccaagttcat attttcctgt cagcaaaccg
    13621 gaagcaagag gaaaataggg aacaaaactg attttgtttt tttcaagata agggaataat
    13681 tctttttcag cctgccgatg gatcaaactg tatttatctt ctactacatc gacatatcca
    13741 tctgcattcg cttctttcaa ttgttctaac gtgaagtttg acacaccgac tgctcggatt
    13801 tttcctgctt cttttaattc gtgcaatgta gcaactgatt cattttttgg tgtgctttca
    13861 tccggaaagt ggatataaaa aatgtcgatg taatcagttt gcagacgttt tagtgcatct
    13921 tccaccgatt gtttgagaaa ctcaggagaa ttatcaaatg ttcgcccttt atttggtaca
    13981 tgagcagctt ttgtagcgat gacgacacgt gagcgatcat attctttttc cttcagtacc
    14041 tcgccaatca gttcttcgga acggccgttt ccgtaagcat aagcggtatc gatcaaattg
    14101 atcccgctat tcaaagctgt acgaactact tgttttcctg tctcatcttc caaaccgtca
    14161 aaaagattat gtcctccaac ggcatttgct cctaatccaa gctgttctgc gtagacttgt
    14221 gaatgtccga ttcttatttc tgccatagct gattcctcct ttttgtctag tctaaaacaa
    14281 aataccaaaa agctgaacta aaacgttttt gtgaggggaa atagtcagat atctcttgac
    14341 tttgagactg gtttttagta ggttataagt aatatcgaat gtcaatgaaa gagcgagtaa
    14401 gtttttgaaa ctttacaaga gaggatcatc gaggctgaaa atgatctaaa gtttcattag
    14461 cctaaagaca ctctggagat ggacgagtga aaagccgtcc cgcgtacgag cgttattcgt
    14521 aagaggaaag agaaatcttt caaaattagg tggtaccgtg tatgaatttc attcaccctt
    14581 gttcatgatg aacaagggtg atttttttat ttacatagct aagaaaggga gagaaaacta
    14641 tgagtttcca aagaccaaaa ggaacaaatg atattttgcc aggcgaatca gaaaaatggc
    14701 agtttgtcga agaaacagca cgtttattat tcagagatta tcagtataat gaaatgcgta
    14761 caccgatttt tgaacactat gaagtgatat cccgaagtgt aggggataca acggatatcg
    14821 tttcaaaaga gatgtatgat ttttatgaca aaggggaacg ccatgtgacg cttcgaccag
    14881 aaggtacagc accaatcgtt cgttcttttg ttgagcataa attgttcggc ccagagtatg
    14941 cgaaaccgtt caaaactttt tatatgggac caatgtttcg ctacgaacgc cctcaaaaag
    15001 gacggttacg tcaattccat caaatcggag tagaagcatt tggatcagaa aaccctgcaa
    15061 ctgatgtgga gagcatggtg atggcacttg atttcttcaa acagttgggg atcacgcaaa
    15121 tccggctagt catcaattca ttaggtgata aagaaacgcg tcaagcctat cgtcaagcat
    15181 tgatcgacta tcttttacct tttgaatcag agctgagtga agactctaaa cgccgccttc
    15241 atgaaaatcc attacgcgta ttggacagta aagacaaacg agatcaaaaa tttgtggcag
    15301 aagctccatc tatcttagat tatctaagcg agcctgctca aaaacatttt gataccgttg
    15361 tttccatgct ggatgcattg gctgttcctt atgaaatcga cagtaatatg gttcgcggac
    15421 tagattatta cacacatacg atttttgaaa tcatgagtga cgcaccaaaa atgggggcac
    15481 aagcaacgat ttgcgcaggc ggacgttatg acaatctagt ggaagaacta ggtggtcctt
    15541 caacacctgg cttcggattt gcaatgggta tcgagcgatt actcttgact atggaggcag
    15601 aggaagtagt tatccctgca tttaatgagt tggatgctta cgtggtcgca ctcggagacg
    15661 aaacaaatat cgaagcattg aaagtggtcc aagcgattcg aaactttggt ttttcagctg
    15721 accgcgactt catgaaccgt aaagcaaaag cgcaatttaa gacagcagat aaagcgaatg
    15781 ccaaattagt attggtgatc ggtgaagatg agcttgctag tcaaacgatt aatgtcaaag
    15841 caatggcaag ccgaaaagaa aaaagttttc cattagaaga gatatatgaa cattttgatg
    15901 aagtctatga tgaaatgact ttattcgagt aggaggaaaa aaataaatgg cacaaagaac
    15961 gatctattgc ggattagttt ccgaacaaca attagaacaa acagttacat taaaaggatg
    16021 ggtacaaaaa agacgagacc ttgggggagt aatctttatc gacctacgtg atcgtgaagg
    16081 aatcgttcaa atcgtcttta atcctgaaat caacaaagaa gcatgggaaa tcgcggataa
    16141 atgtcgcagt gaatacgtga tcgaagtgac tggggaagtt agaagacgcg gagaaaatgc
    16201 agtaaatcct aaaatggcca ctggtgaatt cgaagtgatt gcaagtgaaa tcgtgatttt
    16261 gaatcaagca aaaaccccgc catttcaaat tgaagatgat caagcaatca gtgacgaaat
    16321 acgtttgaaa tatcgttatt tggatctgcg tcgtccaaaa atgactaaaa acatcaaatt
    16381 aagaaatgcg acaacaaaag cgattcgtca ttatttagat gataatgact ttctcgatat
    16441 cgaaacacct tacctaggga aatctacacc agaaggcgca agagactacc tagttccttc
    16501 acgtgtccat gctggacatt tctatgcgtt gccgcaatct cctcaattgt ttaaacaatt
    16561 gctgatgaat gctggattcg accgttatta tcaaatcgtt cgttgtttcc gcgatgaaga
    16621 tttacgtgga gaccgtcagc ctgaatttac gcaagtagat attgaaacaa cgttcttaac
    16681 agcagaagaa atccaaactt acacagaggg attgattgcg aaggtaatga aagaagttcg
    16741 cggtatcgaa gtcacattgc catttcctcg tatgacctat gatgaagcaa tggctcgcta
    16801 cggcagcgac aaaccggata cacgttttga tatggaattg atcgatctaa gcgatacagt
    16861 caaagaagta gaattcaaag tcttccaaat ggctcttgaa aacggcggtg tcgtgaaagc
    16921 attgaatgct aaaggcgctg cagatcgcta ctcacgaaaa gacatggatc agttaggtca
    16981 atatgtagga caattcggag ctaaggggct tgcatggctg aaagtagaag aagacggatt
    17041 gaaaggtcca attgctaaat tcatgggaga agcaacagaa gcaatcatta aagcaacaga
    17101 tgcaaaacca ggtgacttgc tgatgtttgg cgcagataaa agtgaaatcg tcgctgctgc
    17161 attaggtgct atccggacac gtttaggaaa agaattagga ctaattgatg aatcgaaatt
    17221 caatttctta tgggtaactg attggccaca atttgaattt tcagaagaag aaggtcgata
    17281 tgtctctgct caccatccat tcacgatgcc aaaagaagaa gatatcccgt tattggctga
    17341 agaccctgca aaagtctatg cagaagctta tgatatcgtc ttgaacggtt atgaactagg
    17401 cggaggttct atccgtattc atactcgtga gcttcaagaa aaagtactag aaactctagg
    17461 ctttacaaaa gaacaaatgg aagaacaatt tggtttcttg ttaacagctt tagattacgg
    17521 attccctcca catggcggta ttgcattagg attggaccgt tttgcaatgt tattagccgg
    17581 agaagataat attcgtgaag tcattgcctt tcctaaaaac ggaaaagcag cagatgtgat
    17641 gagtgatgca ccaagtatgg tttcgccttt acaattgttc gaattgaata ttgatgtaac
    17701 gaatatagaa gaatagccga taaaaaggta aaaaggattt gtcgaatacg cttcttttga
    17761 caaccttgat ttaattgaaa aacactttat aatgaagcca taacatataa agcggggctt
    17821 ttccatgaaa aaaacattcg aaaattgggc ttatcatgat tacacgacca agctttcggt
    17881 atctatcgtt tatgcgttga tcgcatcagt cgcattgaat ttcttttatc agccaggaca
    17941 tatttattca agtggtatta ctggtctagc tcagattttg acgactttgt caaaatcact
    18001 gataggattc gaagtaccgg tatcaacaac tttgtattta ctgaatatac ctttgtttgt
    18061 attggcgtgg ttaaaaatcg ggaagaagtt tacaattttc acttttatca cagtcacttt
    18121 gacctccctt tgtatccata ttttaccgga aacagtccta tctaccgatc caatcatctg
    18181 tgcgttgttt gggggggctg tgatgggact aggtattggg ctagcgttga gaaacggcct
    18241 atcatcgggc ggtctagata tcgtgagtat cacgattcgt aaaaaaacag gaaagtcgat
    18301 tggttctatt tcgatctact tcaatgcatt gatcgttttt gttgccggaa ttctatttgg
    18361 ctggcaatat atgttttaca gtacgttgtc tattttcgtc agtggaaaag taactgatgt
    18421 cgtttatact aaacaaaaga agatgcaggt aatgatcgta acgaaacatc cagaaaaagt
    18481 agtaaatgaa atccaaaact gtatgcgtag agggatcacg atcatcaatg aagcagaagg
    18541 tgcttataag catgataagc agacagtatt gatgacgatc gttacacgat acgaactgcc
    18601 tctacttaga gaagcaatga aagcttctga tccacaagct tttgtaagta ttgcagaaaa
    18661 cgtccagata cttggtcgtt tctatgagga tggcatttag atcgagtttg acaaaaattc
    18721 atgaaatact gctgtagaca gacgttcgtt tgtctgcagt ttttttctac catagtagaa
    18781 tcagcaaaga aaaactgctt ttttatttga aattttctta aagttgaaaa aaagcatgtt
    18841 tattttttta aatttatggg atgatagaaa aaaatagtag agaaaggaca cgattctact
    18901 tgctattttc acttaatacc cgtatattac gaatgaaaaa agtttatgaa aagttggaat
    18961 attgaatgga aaaacaaaaa aggttaaaaa acagccgcta catcactggg tttgatgggc
    19021 tccgttcatt agctgtggtg ggtgttatct tctatcacct tctgccaact agtatgaaag
    19081 gcggctattt gggcgttccg atttttttcg tcgtatctgg ttatttgatc acggatctgc
    19141 tgcggcagga atgggaacaa aatggaaaaa tcaatatttg gcaattttat gtcagaagaa
    19201 tgaagagact ctatccggca cttgttttta tgcttgtgac agcttcggct tatatcactt
    19261 tgtttcagcg agggctgtta aacaatcttc gaggagttgt ggtcagcagc ttactgtatg
    19321 tcaacaactg gtggcaaatc aataacggtt tatcttattt cgatcgattt gctaatgagt
    19381 caccgtttac gcatatctgg tctttagcag tagaaggtca aaactatttg gtttggccga
    19441 tcttgtttgt attgttgatg gtatttgttc gtaaaagaaa atggatcttt tatactgtgt
    19501 taggtacatc cttcctctcc gccatcttga tgatgatctt gtatacacca ggaggcgatc
    19561 caacccgtgt ttattacggt accgacactc gtctgttttc tatatggttg ggaagtgcgc
    19621 ttgcgtttgt ttggccaagt acgcgtttaa agaaaaacat cccgaaacaa gcgaaaaata
    19681 ttctgaatat cgctggagga atctcgcttt tggcactgat tctttgcttc ttctttttag
    19741 atgatcatta caatttcatc tattacggag gaatgctcct agtgagtctg ttatgcgtag
    19801 tgctagttgc agtgacagca caccccggag caagtttgaa tcgatggctg acaaatccag
    19861 tatttacttg gattggcaaa cgaagctatg gcatttatct gtatcagttt ccagttatga
    19921 ttttttatga agcaaaggtg actgatattg ccgatcatgt tttgttgcat actttagtgg
    19981 aaatcacatt gattctaatc atcagtgaac tttcttatcg atatgtggag cgtccgctag
    20041 cacgttttga ttatggtcgt acattccaag tgatgaaagg atggttcagc aaaccgatca
    20101 tcagtaagag caaaccatgg gttgttccag gcacattact tgtagcaatt gcactcgttg
    20161 gtttcgttac ggcaccgaaa aacgctgtaa cagccgatca aaaacaactg caggcaaaaa
    20221 tcgctgaaag taaaaaatta gcagaagaaa ccagaaacaa ttcagaaaac accactagtt
    20281 cggttgacca agcgatcctt gataagtacg agttgtccaa ggcacaaggg aataaggcac
    20341 aaaaattaga actaacggca tttggtgatt cagttatgtt ggatgctgca acggatctac
    20401 aagaacttta tccaaaagta gtggtagatg gagatgtcgg acgacaatta tatgccagtg
    20461 taccgtacat tgaaaaatta aaggaacaag atttgttgaa agacactgtt ctgatcggac
    20521 taggaacgaa tggctcattc tcggaagctc agtttgatag tgtgatggat gcaatcggtg
    20581 atcgaaaagt atactgggtc aatgtccgag taccgacgaa gcgctggcaa aatgacgtga
    20641 atgcgatgct tgaacagatg acgaaaaaat acgacaatat gacgctgatt gattggtaca
    20701 gctacagtaa tgaccatagt gactggtttt atgatgatca agtccatcca aatcctgaag
    20761 ggatgacaca ttacattcac ttagtcagcg aagccttgct aggaggaagt tcaagtacga
    20821 acagcagtga gacaaacgtg acagaatgat agctacaaaa aacttcaggc aaaaacattt
    20881 gtttttgcct gaagtttttt tatatttacc gtgtttttaa cattcatata gattcaggaa
    20941 gcaaaacggt tgagtgtgtc tgtaaataac gttctttttg ttttcgggtg agttttttga
    21001 atgcagcttc tgcttttgtt gcttcgcttc ttgaagaaaa tacttctgca tggatcatct
    21061 taacaggtct tcttttttga agacgagtat atttagcacc agtaccattg ttatgttcct
    21121 ctaatcgtct agaaagatcg gttgtatatc caccataaaa tgtctgatct ttacagaata
    21181 atacgtaaaa ataatgttca tttgccatag agcatctcac gaatctctgc agtgtattct
    21241 ccatccatat tgtaagtgat caatggtgga gcgactttga atccgtctaa tttgccttct
    21301 ttgattcctt caactaacaa aatatttgcc tcttttccag gttttgggta aacaaattgg
    21361 atccttttag gtgcaattcg atagtcttcc atgacctgaa tgatttctaa gaaccgatca
    21421 ggacgatgga ccatagtaaa gcgtccattt gtttttagta gcttacttga aatatcgatc
    21481 acttcttcta acgtcgtatg aatctcatga cgggcgattg ccaggtgttt attagggttc
    21541 gttttatttg ttggcagccc tttgaaataa ggtggattac agacgactaa gtcacaagaa
    21601 ttatgtcgaa cgacagataa gctgtctttc agatcgatgg tgtggacagt catttgttca
    21661 tttaaatggt taagcgcgat actacgtctt gccatgtctg ctagacgagg ttgaagttcg
    21721 atagcgtcga tagccgcctg ggttcgttta cttaaaaaaa gtccaactgc gccatttcct
    21781 gcacaaaggt caacaatctt ccctttttta ggtacacggg aaaagtgagc aagcagcaca
    21841 gcatctaaag aaaaagaaaa cactttggaa ctttggataa tcttgatatc ttctgcgtat
    21901 aattggtcga ttcgttcgtc ttcgtgaagc atggattccc tccaaaatga aaaaaatagt
    21961 cgtctgtatt atactatttt tattaaaaag aaccaaggtg gcttgtcaaa cctctatttt
    22021 ttatgcatac tatgaccata agaagggaaa ggaagagact atgttttatc gatttatgcg
    22081 cggggtagtt cgagtactat tagccttagt taatggaaat gcccgctatg agaataaaga
    22141 agttttaccg caagatgaga attacatact agttgctccc catcgtactt ggtgggaccc
    22201 attatatctg gcggtagctg cagcacccaa ggaattcagc tttatggcaa aagaagaact
    22261 gttcaaaaat ccagttctgc gatttatatt aactcgatcc aatgcttttc cagttaaaag
    22321 agaaaatcca ggaccaagcg tcattaaaac acctgtaaag attttgaaat cgacaaattt
    22381 gagtttgatc atgtttccca gtgggacaag acatgctaca gaacttaaag gcggaatggc
    22441 attgatttcc agaatgtcta aagcgaaaat cgtgccagct gtatatcaag gtccgttgac
    22501 acttggggat ctgttcaagc gtcagagagt gacagtgcgt ttcggcgaac caatcgactt
    22561 atcagatatc aagaaaacgg ataaagaagg tatggcggag atcgaacgac gaacacaagc
    22621 agcttttgac aaattggaca aagaagtgaa ccctgatttc aaatatgaag tcaaaaaaaa
    22681 ataaaatgat aagtttatgc gtgggcggtc ttttgaatct actgtcctgt tcagttcaga
    22741 ctttttcaat gaaaacgagg gagagacgtc aagtctgccc tctttttgcg ttccaatatg
    22801 taaaatagaa gacaggtgtc atgtaatgga tcaaagtgtt ataataatta gaaggacatt
    22861 ttttgaggtg attgaatgcg ttttttacat acagccgatt ggcatatcgg aaaaaaacta
    22921 caaggttatg acttgattga agaccagcgt catgtactat cttctatact agatatcgcg
    22981 aaagaagaaa aagtagaggc aatcgtgatt gccggagact tatatgatag gagtgtccca
    23041 gctgttgatg ccgtagaatt atacaatgaa ttgatggtcg attggaattt aaaggaaaag
    23101 atcccgattt ttgctatatc aggaaatcac gatagcagta cgagactttc agcgggtacg
    23161 ccatggttca gttattcgaa ctattttttg cgaacacagc tagcagaagc atttgaacca
    23221 gtcgagttta aaaacgtaca atttttcttt cttccttact ttgaaccaat tgcagcgaga
    23281 ttatattttg aagatgacaa tatccgaacg atcaagcaag cggtagccaa ggtgatagat
    23341 aaaatgcagg agctatttga ttcagaaaaa aaacaagtat tgattagcca tttttttgta
    23401 atgggaagtg taaaatctga ttcggaaacg aaaatcgaag taggcgggtt ggattcgatc
    23461 cctggtgaac tacttacacc gtttgattat gtagcactag gacatttgca taatcaagct
    23521 gctttgagac aggataatgc tcgctatagc ggttccccat tgaaattctc tttatctgag
    23581 atcaaccaaa aaaaaggcgt atggatcgtt gacctgacaa caggcctgga gttgaatttt
    23641 agggagatcg aacccctgca tgatattgaa gaaataacag cgagtttcaa agaattgctg
    23701 gatcctgcct tttatcagtc cgtcaatcgg gagaattaca tctatatcta tctcactgat
    23761 agaacgatca tcccgaatat gatgaaccag cttcggcaaa tatatcctcg tattttgggg
    23821 attgaacggg caaacggtcg agcagaacaa acaaagcgca aacgaaaagt actagaaatg
    23881 aaagatcctc aaaaattagc agaagcattt tttagtgaaa tgacggaaga gcagctaact
    23941 gcaaaccaag aaaaatggct cggagaaacg ttgcaagaaa tcatggaaat ggaatgatca
    24001 agaatgaaac cgaaaaaatt acaacttaaa aattttggtc cttttatcaa tgagaccgta
    24061 gatttttcac gtttgacaga agcaccgctt tttttgatca gcgggaagac aggagctggc
    24121 aaaacgacga tttttgatgg gatcaccttc gcactttttg gtgaaacttc cggaagactt
    24181 cgctctggaa aagagatgcg ttctttgttt gctacaccag acgaagaaac aagcgtgact
    24241 ttttcttttg aacatcagca gatgagctat gaaatcgaac gaaaaccaga acaagtttta
    24301 gcaaaacgaa aaggaaacgg aacaaaaaaa caggcagcca aagtaacgtt gaccattttt
    24361 gatgggaaag gaaaagaaat aaaacaaatc acaaaacgta cagaagcaga tcagttgatc
    24421 agagaactga tgaatctcga tgcgaaacag ttttctcaaa tcgttttact accccaagga
    24481 gaattccgta attttttggt ttcatcaagc agtgataaag agactgtttt acgtcattta
    24541 ttcggtactc aatttttcca acagttcaat gagcgattaa aagaaaaagc aaaacaacag
    24601 caacaaaaat tggatcattt agagcaagag ctgcatcttc tgcaaaaaag gtttgtttta
    24661 gtcaaagaag aggaaccagc agcagggttt gaagccgttt tagcccaatg gacaaaccat
    24721 caggcaatac tcgaaaaaca aatcacggaa gaaaaagaag ctctaacgga acttcaggaa
    24781 aaacaaaagc aactagaaaa ttcctactat cgttttgaaa gcttgaaaaa aagttatgaa
    24841 gaaaaaacga tattaataaa caagcaaaag gaattagctg ctcaaaaaga cgaaatagag
    24901 gaaaagaaac gatggattgg gtattatgaa tttacgcaac gattagtcga accaatccag
    24961 cgcctacaag aaataaaagt tgaaaaaata gaactgtcac aaaaaagtag tgagcaaaca
    25021 aagaaactag cgatgactac gcagaattat gaagagtggc aaaaaaatga agcaaaccgc
    25081 gtacgccgac aaacaaaaat caaagaagct gctcaaaaac tgcaaaacaa acagatgatg
    25141 gtgccaattg tcgaatcctt acgtagtaaa aaacaagacg cagaacaatt aacaatttca
    25201 ttggataaag aagaattgaa tgccgagaag ctgtatcaaa atctcgctat ctatcaggaa
    25261 agaactcgaa ttcttcaaga aaatttactg gatcaggaaa aactgcaaga agatcgtctt
    25321 gcttttggac agttaaaata tctagatgaa aagcagcaag aaatagaaaa acaaaaagca
    25381 aaagacactg ggtcgctcgc acagataaga gaagaactca aaaaagcaac tgaagaaata
    25441 acaaaaaata gtcagctatt gaaagaacag acagaacaag cagaacagct gaaaagccaa
    25501 tgggcaaagc agcaaattgc tcgtcttcag ctttttttgc gtccagggga gccatgtctt
    25561 gtttgtggat caacggaaca ccccatacaa caattaaaac acgaacagcc aaataaggaa
    25621 gatatcttaa aagtagaaca acaactggca gaagctgagt ctgctgtaga aagaactcag
    25681 caaatcattg ccaaaagtga agcacatgaa aataatcttc aacaacaatt atcagattta
    25741 gaacaaaaaa tcatggcaac gaatatacaa ttggctgaac aagaagaaca cttgcaacag
    25801 gctttgaaaa aggcatttcc tgagataaca gaacaaaaaa caaaaacaac tcttgtagta
    25861 atcgaacaat ctttagatag acgaaaaagc aagatggcca atgcaagaat agagctggaa
    25921 gaaataagac aacaagcaga aaagctcgaa caacaattag ctgtctacaa acagcagcag
    25981 acagaaaaaa ataatcagct ccagcaactg caaggagaaa tcaaaagttt agaacaacaa
    26041 ttgcaagata cggatagtaa ctgtttagaa gaggagatac gacgacttga aaaacagtta
    26101 gaagaagact cgaatcaact agctgttgag cagaaagcag gagtgcaatt gttgcaagat
    26161 cttcatacgc ttcggacaca gcttgatctt caacatgaac aaatagaaaa aatagatgaa
    26221 aaaatagaaa aggaacaaga aagaatagct aaaaagttag aaagccagtc gtatttttca
    26281 acaattgaag aaatctgtgc atttgcacaa cagcaagcac aatataaaga aaaccaacgt
    26341 acagttactt cttatcatga agagcaatta gtgacagctg accggctgcg tcaattagaa
    26401 aatatccagc taactgagac attcccagat acagaagaga tcagccaaca gttagaagaa
    26461 ataagacgta tgatcgaaat aaagcaagaa aagatatacg gtttacaaga acaaaaaatt
    26521 agtaatcaaa aaatctacga tgaatgtctt tcgatttatc acacaagcca gacgaaatta
    26581 gatgagctta cgcaattaca gcagctttcg caaacattca acggagaaaa tcctaagaag
    26641 actagtttgg aacggtatgt gcttcaggtt tatcttcaag aagtgttaca agtagcaaat
    26701 gatcatctcc aacgtttaac gaaaaatcgt tatcaatttg agctagcgga taccattggc
    26761 agttatcgag ggaaaaccgg attagaaatc aatatctatg atgatcaggc aggtatgtct
    26821 cgaagtgcgc ataccctgtc tggcggtgag agttttattg cagcactttc tttagcatta
    26881 gctcttgccg atgtgatcca gacacaagct ggtggtgtag caatcgatgc attgtttatt
    26941 gatgaaggat ttgggtcatt agatgaagaa gctttggaaa tggcaatgga agctttagaa
    27001 acgatcgaaa gtgaaggaag aatgatcggc atcattagtc atgtaagaga attgaaagaa
    27061 cgagtattac aacaaataag aattgataca gaaggtagcg gacaaagcaa aatacgctat
    27121 caacttggat aaaaagggag attggtaact atgaaatgga gtattccaga aaaagtcatt
    27181 gcacgtggac gtcaatactt gcaagaaggc cgagtgttat cggttgtccc tgaccaagaa
    27241 gaaaacgtgt ggcatgcaga agtcttgggc agtgaattgt atatggttga tttggatggt
    27301 acagcaaaag agatagatta ctgccaatgt ccctattggg aggaacataa atattgtaag
    27361 catacagttg ctgtggaact ttatttacgg tcgaaaaatc tatctcgtat catgaaaaaa
    27421 gaccagccaa tgctagaacc ggtaaaagca acaagtgagg gagaaatcat tgcaaaaggt
    27481 ttttctcgtc taaaaacaca aaacaatcct caagcggtgc aacctttgat tattgaatac
    27541 caagtagaaa cgattgagac taatcagtat cacccagagc tttctatttt ggcactttac
    27601 ttgcgtatcg gttcattggc atcaccgaaa aaaacatata tcgtaaaaaa catctatgat
    27661 tttttacaag cttacacaga aaaagaaact tacactgtta ataaacagta ccagtttcgt
    27721 ctagataaac gggcgtttca aaaagaagat caaaaaatac tcacgcatct tgcaggaagt
    27781 gctcagacgc atcaattatt aggtacgaac ggcttacaag tcaaaggaaa actagataaa
    27841 agatatctgc ttctcccagt agaacaagcc cgcttcctgt tagagcagat gaatcagact
    27901 acacgtttgc atctgcagat aaatgaacaa aacgatcaag gaatacaatt ttccgatcaa
    27961 aacaaacctt tatcattcaa agtggaaaaa tttggagaag attatttgct gcaagcattg
    28021 aatgattttg attttttctt tatgcattat cagtggggag ttatccaagg gacattttat
    28081 tctctgacga aatatcaaca aacggtttat caaacttgga aacaattgat tcgccgattg
    28141 gagaaaccag aagtagtgtt caagaaaaaa gaattaccgt ctttgtttaa agagattatc
    28201 cctctgctgt cagagatcgg cgacgtagct attgcccctg cagtagaaga ggaagtagca
    28261 gatatccccg tcgaatttat tttctttttc cgaaaagcaa aaggaaaaat ccaagctcga
    28321 gtcgatttcg tctatggcga agtcatctat tcgactgacc cgaagcatga ggtctcgaca
    28381 gaacaggctt ttcaggtgtt acgtgacaca gtagaagaga aacgagtact tgatctattc
    28441 agaatgtatc gctaccagaa aaatgaaact ggttatgaac gtccattgcc aagcggggaa
    28501 gaactatatg cctttttccg cacagaacta tctgtcttcc gtcagtttgg tgaagtcaga
    28561 cttggaagaa aattgcgaga gttgtattta gatgcgcata aacatcaacc gaaaatcgaa
    28621 gtaaccgaga ccggttcatg gctcgatatt cgattcgatg tgacagggat agaggagcaa
    28681 gaaatcgatc atgtcctgca aagcttgctg agaaatgatg ctttttatac attagaaaat
    28741 ggtcaggttc tttcttttga ttcagaagaa tttcaacaaa caagccaagt gttgcaacaa
    28801 ttacgggaat cgatacgtac agaagaagga acgatccatg tacctaaaaa tcaaggattg
    28861 atcattcaaa atcaattgga aaaaagcaat gctactttta gtgaatcgtt ccaaacgatg
    28921 gtacaagatc tcattcatcc agaaagatac caggcacagc ttcccaaagg attgaatgca
    28981 acgatgagag attatcaaaa gcaaggtttt cgatggttga agatgttagg tcattatcag
    29041 tttggcggta ttttagccga tgaaatggga cttgggaaaa cactacagac aattgccttt
    29101 ttactttcgg aaaaagaaga acggaaaagc ttctctgctt tgatcgtagc gcctgcgagc
    29161 ttaatttaca actggcaggc agaggtgaga aaattcgccc cttctttaag tatccaagta
    29221 atcaatggga ataaaaaaga acgtgaagaa ttactggcta aagacacaga tatccgagtg
    29281 acttcttatg ccagcttaag acaggatcta gctaattatc agtcgcaaaa aattgattat
    29341 ttgattcttg atgaggcaca aatggtcaaa aatagcagta cgaaaactgc acaagcattg
    29401 agagaattag ctgtacctca gcggtttgca ctgagtggga caccaatcga gaataattta
    29461 gaagaattgt ggtcattgtt tgcaacgatt atgccaggat ttttcccgac taaaacaaaa
    29521 ttcagggaac ttgccgcaga agaaattgcg caaatgattc gaccgtttat tttacggcga
    29581 gataaacaaa ctgtgttgaa agacttgccg gaaaaaacag agatgaatct atattctgca
    29641 cttacagaag aacaaaaaac tgtttattta gcttacttgc gtcaaatgcg tgaagaaatc
    29701 acatcaatgg attctgaagc attcaagaaa aaccggatcg ggatacttgc tggtctgaca
    29761 agactgcgac aaatttgctg cgatcctcgt ctgtttattg aagattacca aggcggttcc
    29821 ggcaagctcg aacaagtaaa agatctactg gtagctgcca aagagaacaa acgaagagtt
    29881 ttgctgtttt ctcaatttac agggatgctg actatcttgc aagaagagtt agccgagcta
    29941 gggatctcga cattttattt aagaggcagt acgaaaccac aagatcggtt aagtatggta
    30001 gacgcattta atgctggcga aaaagatgtt tttctgattt ccttaaaggc tgggggaact
    30061 ggattgaatc tcacgggagc agatacggtc atcttgtatg acctttggtg gaatcctgcc
    30121 attgaagaac aagctgctgg acgtgcacat cggatcggac aaaaaaatgt ggtagaagtg
    30181 tggcgtatga tctctgaagg aacgattgag gaacgcatgg attcactcca gcaagaaaaa
    30241 cgcgaactgt tccaaaaagt gatccaaggt aatgaagaac agttgacgaa actgactgaa
    30301 gaagacatcc gcatgatttt aagtgtagga gaaatcgagg agtaagagac tcgaaaaagc
    30361 gttcagtaat gggtaagaaa gcctttctaa agaagcgatt gtttatttga tttaaaaaat
    30421 agcaaggagg ctgggattcc attttatccc agcttccttg ctatcatttt tttatttcta
    30481 tttttaaagg ttatacaaag attgtcttca ttgtctctaa cagctgatca aaagaagaaa
    30541 tctcgatatc gcaaattgga gtctgttcaa ttttgcgttc tcgatggttg aaccaaagcg
    30601 cttgccagtt agcacttttt gcacctagaa catcattgtc gtaattatcg ccaacgtata
    30661 aggtctcttc tggaagcata tgaaattctt tctcagctag ctgaaaaatc tctttttctg
    30721 gtttttggaa acctgttgct tgagaaatga gcatattgcg ggaaggaatc caattgttca
    30781 attgaagttg cttcagtttt tttgtctgat ggtcagtcgg cccatttgtg ataattccta
    30841 atgggacatt cttttccttc aagaaatcga gggttttttt gacctcctca tgcaagcaga
    30901 tattatcaag ttcttcctcg taaatttttt gaaataagag gccatcttct tccgtaatat
    30961 gagggtagtc taaatctttt agtgattggc tgatccgatg tgctcgcata tattctagtg
    31021 tccaatctcc tgccattact tttgggaagt tttcatcact atgatggcgg aaccggatat
    31081 atagtggatg catatctgca tctgaaacaa gcggaacgac tcttttgacc gcgtttcgaa
    31141 atggctgctg ctgatcatac atcgtatcat caacatcaaa cacaactgat ttaatcaaaa
    31201 ttttacacgc ctttcggttt tcattgattt tttcacaaat ccttgactat tgtaacagaa
    31261 aaaattgtct aaaaaagctg ataaataaag gtttttctta ctatatttct atttccttga
    31321 cagaataagg tttttatatt tcagtttttt tcactaaaaa tcgtgaaaca cgtcaataag
    31381 tttgatgaac aaattaatca ttatggtaga agtcgattga tttattttaa tgcaatctct
    31441 gtggaatttt cgaaagattt atttttttaa tacagtctca atataactat tcaaacataa
    31501 aataacgtta tttgatttat ttgtttatag taaattttat ttagtaatca ttgctttaaa
//
LOCUS       DACEZN010000016        28140 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEZN010000016 DACEZN010000000
VERSION     DACEZN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.016"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(82..777)
                     /locus_tag="IXH60_000565"
     CDS             complement(82..777)
                     /locus_tag="IXH60_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1989530.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(867..1967)
                     /gene="ychF"
                     /locus_tag="IXH60_000566"
     CDS             complement(867..1967)
                     /gene="ychF"
                     /locus_tag="IXH60_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1989531.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1976..2167)
                     /locus_tag="IXH60_000567"
     CDS             complement(1976..2167)
                     /locus_tag="IXH60_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1989532.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2188..3078)
                     /locus_tag="IXH60_000568"
     CDS             complement(2188..3078)
                     /locus_tag="IXH60_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1989533.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3065..3832)
                     /locus_tag="IXH60_000569"
     CDS             complement(3065..3832)
                     /locus_tag="IXH60_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1989534.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="IXH60_000570"
     CDS             complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="IXH60_000570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1989535.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="IXH60_000571"
     CDS             complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="IXH60_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1989536.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="IXH60_000572"
     CDS             complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="IXH60_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1989537.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8448..8786
                     /locus_tag="IXH60_000573"
     CDS             8448..8786
                     /locus_tag="IXH60_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989538.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(8954..9712)
                     /locus_tag="IXH60_000574"
     CDS             complement(8954..9712)
                     /locus_tag="IXH60_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1989539.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9730..10548)
                     /locus_tag="IXH60_000575"
     CDS             complement(9730..10548)
                     /locus_tag="IXH60_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1989540.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="IXH60_000576"
     CDS             complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="IXH60_000576"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1989541.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="IXH60_000577"
     CDS             complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="IXH60_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1989542.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11676..13010
                     /gene="dnaA"
                     /locus_tag="IXH60_000578"
     CDS             11676..13010
                     /gene="dnaA"
                     /locus_tag="IXH60_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1989543.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13208..14338
                     /gene="dnaN"
                     /locus_tag="IXH60_000579"
     CDS             13208..14338
                     /gene="dnaN"
                     /locus_tag="IXH60_000579"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1989544.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14567..14809
                     /gene="yaaA"
                     /locus_tag="IXH60_000580"
     CDS             14567..14809
                     /gene="yaaA"
                     /locus_tag="IXH60_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1989545.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14796..15920
                     /gene="recF"
                     /locus_tag="IXH60_000581"
     CDS             14796..15920
                     /gene="recF"
                     /locus_tag="IXH60_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1989546.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15917..17863
                     /gene="gyrB"
                     /locus_tag="IXH60_000582"
     CDS             15917..17863
                     /gene="gyrB"
                     /locus_tag="IXH60_000582"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1989547.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17883..20354
                     /gene="gyrA"
                     /locus_tag="IXH60_000583"
     CDS             17883..20354
                     /gene="gyrA"
                     /locus_tag="IXH60_000583"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1989548.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20590..20889
                     /locus_tag="IXH60_000584"
     CDS             20590..20889
                     /locus_tag="IXH60_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1989549.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20939..21451
                     /gene="ssb"
                     /locus_tag="IXH60_000585"
     CDS             20939..21451
                     /gene="ssb"
                     /locus_tag="IXH60_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1989550.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21477..21713
                     /gene="rpsR"
                     /locus_tag="IXH60_000586"
     CDS             21477..21713
                     /gene="rpsR"
                     /locus_tag="IXH60_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1989551.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21856..23829
                     /locus_tag="IXH60_000587"
     CDS             21856..23829
                     /locus_tag="IXH60_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1989552.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23835..24287
                     /gene="rplI"
                     /locus_tag="IXH60_000588"
     CDS             23835..24287
                     /gene="rplI"
                     /locus_tag="IXH60_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1989553.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24561..25928
                     /gene="dnaB"
                     /locus_tag="IXH60_000589"
     CDS             24561..25928
                     /gene="dnaB"
                     /locus_tag="IXH60_000589"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1989554.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26708..28000
                     /locus_tag="IXH60_000590"
     CDS             26708..28000
                     /locus_tag="IXH60_000590"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1989555.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         8574 a         5515 c         5591 g         8460 t
ORIGIN      
        1 tattatttaa ctatggataa acaaaatgag gttctttact acccctagat atctagcaaa
       61 atagctgatc tttttgctag tttatcttct tgggacggat aaacttccaa taatcccgta
      121 ttttttcttc aagtagtaac ggattgctaa agcagttgcc ccgataatga taactactac
      181 tggatctaat actggattga taactggtgg caatagtgtt gtcgcagaaa acagtacgac
      241 ccaaagaagt gtcgttccac ctaggataag caatgtctta cccattcctg gtttcttgct
      301 tcgatctgct ccgggatatt catattgata gatgtatttg tacatcatat aaaagaccca
      361 gccaccgatt gcagaaccta gaacgagtgt agaaaagcca taagctggcg ttgttccacg
      421 tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt
      481 atctaaccac atcaataaag gggtggatga attttttact tctgctggct tattgatgat
      541 tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg
      601 ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa
      661 attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa
      721 ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat
      781 ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat
      841 attctacagc tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg
      901 tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc
      961 atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt
     1021 tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc
     1081 caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa
     1141 gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc
     1201 gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc
     1261 acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct
     1321 gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat
     1381 aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat
     1441 tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct
     1501 ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag
     1561 tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct
     1621 tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact
     1681 tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg
     1741 attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt
     1801 tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc
     1861 gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt
     1921 gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact
     1981 ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca
     2041 tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga
     2101 cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt
     2161 cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc
     2221 aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt
     2281 tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg
     2341 gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt
     2401 ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc
     2461 acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg
     2521 tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa
     2581 gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg
     2641 tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag
     2701 atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc
     2761 tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc
     2821 ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac
     2881 acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg
     2941 gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt
     3001 tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa
     3061 tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc
     3121 ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac
     3181 gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt
     3241 ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga
     3301 tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta
     3361 atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa
     3421 atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact
     3481 gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca
     3541 attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt
     3601 cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc
     3661 ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat
     3721 ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg
     3781 ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc
     3841 ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt
     3901 tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac
     3961 cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa
     4021 ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg
     4081 caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa
     4141 tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc
     4201 atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc
     4261 aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc
     4321 ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt
     4381 aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt
     4441 catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg
     4501 atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa
     4561 tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa
     4621 atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc
     4681 gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt
     4741 gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc
     4801 actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg
     4861 gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt
     4921 ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt
     4981 tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc
     5041 atatttgatt tggatttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg
     5101 aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag
     5161 tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt
     5221 tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt
     5281 caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa
     5341 tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa
     5401 acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc
     5461 gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc
     5521 ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc
     5581 tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac
     5641 tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat
     5701 agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata
     5761 gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc
     5821 aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat
     5881 ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc
     5941 tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa
     6001 gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt
     6061 acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc
     6121 ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt
     6181 caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt
     6241 ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat
     6301 cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt
     6361 catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc
     6421 ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc
     6481 tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg
     6541 attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc
     6601 catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac
     6661 gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac
     6721 aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc
     6781 ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat
     6841 caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc
     6901 gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag
     6961 aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg
     7021 gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt
     7081 tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg
     7141 ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt
     7201 ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg
     7261 tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag
     7321 tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac
     7381 ttgatttgcc gacatttggc cggccgatga ttgccgtact taacccttcc cgcagaacct
     7441 tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt
     7501 caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta
     7561 cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca
     7621 gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca
     7681 aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg
     7741 tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat
     7801 tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct
     7861 ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa
     7921 cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt
     7981 gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg
     8041 caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag
     8101 ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat
     8161 gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata
     8221 gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg
     8281 ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt
     8341 attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact
     8401 aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca
     8461 gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag
     8521 ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag
     8581 ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat
     8641 taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc
     8701 agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc
     8761 aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa
     8821 gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat
     8881 aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca
     8941 aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt
     9001 cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt
     9061 tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt
     9121 cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta
     9181 cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat
     9241 attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt
     9301 ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat
     9361 caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac
     9421 ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct
     9481 cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt
     9541 tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac
     9601 ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag
     9661 ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta
     9721 cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct
     9781 ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat
     9841 cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct
     9901 ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat
     9961 tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat
    10021 tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa
    10081 atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg
    10141 catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa
    10201 acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg
    10261 ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa
    10321 atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc
    10381 tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc
    10441 ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac
    10501 caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc
    10561 tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca
    10621 taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt
    10681 tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc
    10741 gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt
    10801 tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct
    10861 tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct
    10921 atctctcact tacgctcata atcataccat aattacgatt gtcctttcat catttcccta
    10981 aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt
    11041 cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg
    11101 aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc
    11161 acctccaaaa tgttcttatc tattttataa acacagacat actttgatag tataacgaca
    11221 aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt
    11281 atttttttct atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa
    11341 taaaaggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga
    11401 tttcctgtgt attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca
    11461 agaggaaatc tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt
    11521 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11581 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11641 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11701 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11761 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11821 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11881 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11941 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12001 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12061 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12121 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12181 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12241 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12301 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12361 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12421 acgagattcc gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg
    12481 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12541 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12601 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12661 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12721 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12781 aagatctcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12841 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12901 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12961 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13021 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt
    13081 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    13141 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    13201 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    13261 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    13321 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    13381 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13441 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13501 agaaaataaa caagtagcga tcacttctgg aaaagcgaat tttatcgtaa atggattaga
    13561 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13621 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13681 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattag ctgtagctac
    13741 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13801 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13861 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13921 ctattcccgt ttgctagaag gaaactatcc agataccaat cgtttgattc cttcaagttt
    13981 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    14041 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    14101 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    14161 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    14221 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    14281 accaacggaa gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca
    14341 atgattttca atgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa
    14401 cagaaggccc taaaatctct tttaaggctt tctgtttatt tttgtttaaa tcaccttttt
    14461 tagattaaat cgtcttatat ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa
    14521 aggtataata gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt
    14581 cattctaaat acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg
    14641 tagtggcgga caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc
    14701 agaaaatcgt cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg
    14761 aacttttttt atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa
    14821 aaaattatcg aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt
    14881 taggagaaaa tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga
    14941 cgcgaagtca tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga
    15001 tgattcaagg agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta
    15061 aaaaaggccg aaaaaccaag atcaaccata tcgaacagaa aaagttaagt acttacgtgg
    15121 gacaaatgaa tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc
    15181 agaccaggcg gaagttttta gatatggaat taggccagat caatccaatc tatctttatg
    15241 atcttgtcca ataccaagca attttgaaac aaagaaacca gtacttgaag caattgaatg
    15301 aaaaaaaaca aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg
    15361 gcagcaaagt attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt
    15421 ctttgcatca gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag
    15481 tcgattcttt agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga
    15541 accaaaataa aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg
    15601 atctttcctt ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa
    15661 gaacaactgc actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacagggg
    15721 aatatccaat ccttttgtta gatgatgtga tgagcgagct ggatgataat cgtcaactcc
    15781 atctgctaga aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc
    15841 atgtaaaaaa taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa
    15901 aaggagaaga aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga
    15961 tgccagtcag attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta
    16021 tatcggttcg actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc
    16081 gatcgatgaa gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa
    16141 cagtatcacc gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg
    16201 acgtcctgca gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg
    16261 tgggtacaaa gtttctggtg gacttcacgg agtaggatct tctgttgtta acgcgctttc
    16321 tacacagctt gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg
    16381 tggagctgta gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt
    16441 tcactttacg ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt
    16501 ggctacccgg atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga
    16561 caaacgagaa gaagaaccag tcatcaatga ataccattat gacggcggga ttaaaagtta
    16621 tgtcgaatac ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg
    16681 tgaacagcaa gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa
    16741 tatcatgagt tttgccaaca atatccatac gtatgaaggc ggaacacatg aatcaggatt
    16801 caagactgct ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga
    16861 aaatgacgac aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat
    16921 caagcatcct gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt
    16981 acggacagtc actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc
    17041 aagtgtgggc cgtcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc
    17101 gaaacgtgcg cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg
    17161 aaaattagcg gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg
    17221 agattcggcc ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc
    17281 tattcgtggg aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga
    17341 agagatccgg tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc
    17401 gaaagctcgc taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat
    17461 ccgtacattg ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata
    17521 cgtctatatt gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt
    17581 acaaccagga aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc
    17641 accaaaacca agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg
    17701 ggaaacaact atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat
    17761 tgcagcagat cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt
    17821 catcgaggaa aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa
    17881 gaatgagtga agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag
    17941 actccttcat cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag
    18001 atggtctgaa acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac
    18061 cggataaagc ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc
    18121 ctcatggaga ctatgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc
    18181 gttatatgct agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg
    18241 ctatgcgtta taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca
    18301 ataagaacac tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac
    18361 tacctgctcg cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg
    18421 ccacgaatat cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg
    18481 ataatcctga tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa
    18541 caggtggttt ggttatggga aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt
    18601 ctattaccgt acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca
    18661 tcgtgacaga attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac
    18721 tgcacagaga caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag
    18781 gtatgcggat cgtcattgac atccgtcgtg acgccagcgc atctgttatc ttgaataact
    18841 tatataaatt gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag
    18901 gtgtgccgaa agttttgagc ttgaaacaaa ttcttgaaaa ctatatcgaa catcagcgcg
    18961 aagtcatcac aagacggaca gtctttgaaa aagaaaaagc agaagcaagg gcacacattt
    19021 tagaaggttt gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt
    19081 ctaaatcaga tgacgaagca aaagcgacga tgatcgaacg gttcgattta tccgatcgcc
    19141 agtctcaagc tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa
    19201 tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa
    19261 gaccagaacg tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg
    19321 gtgataaacg acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt
    19381 taatcgaaga agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg
    19441 caaataatga attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc
    19501 acaatgacga cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct
    19561 ttacgaataa cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgca
    19621 cagccaaagg aataccaatt atcaatttac taggtatcga ttcgagtgaa agcatccagg
    19681 caatcattgc ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc
    19741 aaggtacagt aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga
    19801 tcgcaattgg tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat
    19861 ctaatatcat tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc
    19921 gcgatatggg acgtacagct tccggggtta gaggaatacg cctgcgtgag gaagattatg
    19981 tcgttggggc agccttaatg aaggaagatc aagaagtatt agtgatcaca gaaaaaggtt
    20041 acggaaaacg gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca
    20101 aaacggcgaa catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag
    20161 atgaagacat catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg
    20221 tttctcaaac tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga
    20281 aagttgtcac gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag
    20341 gatcagaaga atagccatca agactaattg tgtaagctag caagaatgac cgattaaaaa
    20401 atctggttat tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact
    20461 gttcattctg tattgctttt gaccaaaata gagtgtataa tcagagcttg tgagtagtct
    20521 aattaattag ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg
    20581 tgaatatcaa tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat
    20641 gaagaagcaa aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca
    20701 gaagttatcg aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac
    20761 cgtgaaggta tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat
    20821 gaatttgacc gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa
    20881 gaagcttaat tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt
    20941 gattaataat gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc
    21001 aagtggtacg gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag
    21061 cggcaatcgc gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt
    21121 agcaaactat gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc
    21181 ttatgataat cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca
    21241 attgctggaa agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc
    21301 tggtcaatca gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc
    21361 caacggtatg cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat
    21421 cgacatttca gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg
    21481 cacaacaaag aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata
    21541 ttgaatatat cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta
    21601 aaatcttgcc acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg
    21661 caatcaaacg cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc
    21721 taggaattga atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat
    21781 aacctctatt cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata
    21841 tatgggagga aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt
    21901 atcctgttta tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc
    21961 gcttcggtca ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt
    22021 tggcttattt taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat
    22081 ctagcttatg tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa
    22141 aaaatcgaat ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag
    22201 gaagaattgc tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta
    22261 gcagataaaa catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat
    22321 attacccaaa accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt
    22381 ttatctattg ataactatgc agatgccatt gataaattgg atgacaaaga gatttcttat
    22441 ttgaactctt tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc
    22501 aaaagaatca acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg
    22561 atggataaaa aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg
    22621 cctatcaccc ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga
    22681 gaagctcaga ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa
    22741 gaggtaaaag aaggagcaaa acctgtttat tatgggggga aatctgctaa gaatgtcaaa
    22801 cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca
    22861 ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt
    22921 ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt acatcgttat caacgagaac
    22981 gagatcattc cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa
    23041 atgcaactaa tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg
    23101 atggtggatt accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat
    23161 aagattgtga tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg
    23221 acttatatcg aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac
    23281 caaagcagca aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg
    23341 gttgtagata caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc
    23401 tatttaaggt cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta
    23461 gcgacatact tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt
    23521 gttgtggcgg cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca
    23581 gatacattgc tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac
    23641 ggcttgattg ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa
    23701 tccttaggtg gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt
    23761 gaggaaacaa aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat
    23821 aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag
    23881 gggacatcaa agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg
    23941 caaaagaagc aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa
    24001 aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag
    24061 atactgtagt cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc
    24121 cttcaaaaca aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa
    24181 aaatcgaatt agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc
    24241 atcatgaggt gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag
    24301 cgatgttcag ctggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga
    24361 aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct
    24421 tttttgggaa cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt
    24481 tattacactt tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt
    24541 gaagacaagg agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata
    24601 ttgaagctga acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg
    24661 ccatggagta catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga
    24721 caatgatcga gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc
    24781 tggaacaagc caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat
    24841 tagcagttcc gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat
    24901 tgctgcgtac attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag
    24961 aagaagttca aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa
    25021 gaaatcgaag cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg
    25081 atcgactagc tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg
    25141 ataaaatgac agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg
    25201 taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt
    25261 ctgttgcgat atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg
    25321 cagaaggttc gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt
    25381 cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac
    25441 cagggatcaa aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca
    25501 atctaggctt gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc
    25561 gtcaacaaga agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag
    25621 tacctgtcat tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac
    25681 cagtgctaag tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct
    25741 tcttgtatcg agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc
    25801 caaaaaatga caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa
    25861 cagttgaact actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag
    25921 aattttaaag aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca
    25981 aggagtaacc gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg
    26041 acaaaagtct tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg
    26101 gtattagctc agcaaacgtc aaaatatgag gaactatttt gacgtttgtt tctctaatac
    26161 tcaggtcaaa acgctgatct tacgacctct tttccttaaa cggtgttctc ctgccaactc
    26221 ctcggtcaca agtcacaata tcatcaaaca tgagaagcag ttttttgaaa ttgctccttg
    26281 tgactcagag ttaaacggca ggctcacaac ctctttctta aacggtgttc aaaagcttgc
    26341 atctgcggta atacgcccaa tcaaacgaaa aatatgagaa gctatttttg tttggctgtt
    26401 cttattactt gatgcaggac agctttttca caacctctga ttcacggtgt tccaaaagta
    26461 gcttcttcga aaaataagtc gaaatttcac aaaaatttga gaagcaattt tcgtacattc
    26521 cttcttattt cttgaagctg accacttctg ttacaacctc ttttttaata accattctaa
    26581 aaagataaag ttcgtttttt aagcgttttt ataaaataaa aataaaaatc attcgatttt
    26641 atttgaaata tgtatctatt attgatacaa tggttcagga ttaaataata tagaacgagg
    26701 tgttcacatg tcatctgtag tagtagtagg tacgcaatgg ggcgatgaag ggaaaggaaa
    26761 aatcacagac tttctcagtg aaaatgctga agtgattgca cggtaccaag gtggcgacaa
    26821 tgcagggcac acaattaagt ttaacggtgt gacttataaa ctgcatttga ttccttctgg
    26881 gattttttat aaagacaaga tcagtgtgat cggaaacggc gttgtagtca atcctaaatc
    26941 attggttaaa gaattagctt atttacatga aaaaggtgta gaaacaagta atttgcgtat
    27001 ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa ttggaccaat tacaagaaga
    27061 tgctaagggc gaaaataaga ttggtacaac gattaaagga atcggacctg cctatatgga
    27121 taaagcagct cgtgtaggta tccgtattgc agacttatta gataaagaaa tttttgaaga
    27181 acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt gtaaaaatgt ttgattcaga
    27241 accacttgat tttgacgata tttttgaaga atattatgaa tacggacaac aaatcaaaca
    27301 atacgtcaca gatacttccg ttatcttgaa tgatgctttg gatgcaggca aacgcgtgtt
    27361 gtttgaaggc gcacaaggtg taatgctgga tatcgaccag ggaacttatc cttttgttac
    27421 ttcttctaac ccagtagccg gtggggtaac aatcggaagc ggtgtagggc cttcaaaaat
    27481 caataaagtt gtcggcgtct gcaaagccta tacgtcacgt gtcggtgatg gaccatttcc
    27541 aactgaatta tttgatgaaa caggagaaca aatccgcgaa gtaggacgag aatacggtac
    27601 gacaactggt cgtccgcgtc gtgtaggctg gtttgattct gtagttatgc gccattctaa
    27661 acgagtttca ggtattacca acctatcttt aaactcgatc gatgtattaa gtggattgga
    27721 aacagtaaaa atttgtacgg cttatgaatt agatggcgaa ctaatctacc actatccagc
    27781 aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa gaattgccag gctggtcaga
    27841 agacattaca ggatgcaaaa cacttgcaga actgccagaa aacgcacgta actatgtacg
    27901 tcgtatctca gagcttgttg gtgtacgtat ctcaacattt tctgtcggac ctgatagaac
    27961 acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa ggataaaaac gacgactgaa
    28021 tcaggacgtt cttaccagag cgagtcgaag tgtttatcta tacgataata ataacttgaa
    28081 cactgtttat tcagaataag ggactgtgac aaaaaccgtg tcacagtccc tttttccgaa
//
LOCUS       DACEZN010000017        27932 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEZN010000017 DACEZN010000000
VERSION     DACEZN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.017"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(119..940)
                     /locus_tag="IXH60_000591"
     CDS             complement(119..940)
                     /locus_tag="IXH60_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1989556.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1037..1315)
                     /locus_tag="IXH60_000592"
     CDS             complement(1037..1315)
                     /locus_tag="IXH60_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989557.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1583..3592
                     /gene="metG"
                     /locus_tag="IXH60_000593"
     CDS             1583..3592
                     /gene="metG"
                     /locus_tag="IXH60_000593"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1989558.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(4071..4658)
                     /locus_tag="IXH60_000594"
     CDS             complement(4071..4658)
                     /locus_tag="IXH60_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989559.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            4833..6155
                     /locus_tag="IXH60_000595"
     CDS             4833..6155
                     /locus_tag="IXH60_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1989560.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            6310..7086
                     /locus_tag="IXH60_000596"
     CDS             6310..7086
                     /locus_tag="IXH60_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1989561.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            7079..7648
                     /gene="rnmV"
                     /locus_tag="IXH60_000597"
     CDS             7079..7648
                     /gene="rnmV"
                     /locus_tag="IXH60_000597"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1989562.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            7641..8525
                     /gene="rsmA"
                     /locus_tag="IXH60_000598"
     CDS             7641..8525
                     /gene="rsmA"
                     /locus_tag="IXH60_000598"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1989563.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            8976..9605
                     /locus_tag="IXH60_000599"
     CDS             8976..9605
                     /locus_tag="IXH60_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989564.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(9729..10838)
                     /gene="ugpC"
                     /locus_tag="IXH60_000600"
     CDS             complement(9729..10838)
                     /gene="ugpC"
                     /locus_tag="IXH60_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1989565.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            11047..11469
                     /gene="mgsA"
                     /locus_tag="IXH60_000601"
     CDS             11047..11469
                     /gene="mgsA"
                     /locus_tag="IXH60_000601"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1989566.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            11792..12325
                     /locus_tag="IXH60_000602"
     CDS             11792..12325
                     /locus_tag="IXH60_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1989567.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKNFKKFSDPR"
     gene            complement(12369..14132)
                     /locus_tag="IXH60_000603"
     CDS             complement(12369..14132)
                     /locus_tag="IXH60_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989568.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(14135..15850)
                     /locus_tag="IXH60_000604"
     CDS             complement(14135..15850)
                     /locus_tag="IXH60_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989569.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(16629..16853)
                     /locus_tag="IXH60_000605"
     CDS             complement(16629..16853)
                     /locus_tag="IXH60_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989570.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            17053..19821
                     /locus_tag="IXH60_000606"
     CDS             17053..19821
                     /locus_tag="IXH60_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1989571.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            19902..20321
                     /locus_tag="IXH60_000607"
     CDS             19902..20321
                     /locus_tag="IXH60_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1989572.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            20333..20803
                     /locus_tag="IXH60_000608"
     CDS             20333..20803
                     /locus_tag="IXH60_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1989573.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            20820..21599
                     /locus_tag="IXH60_000609"
     CDS             20820..21599
                     /locus_tag="IXH60_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1989574.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            21586..22407
                     /locus_tag="IXH60_000610"
     CDS             21586..22407
                     /locus_tag="IXH60_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1989575.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            22424..23473
                     /locus_tag="IXH60_000611"
     CDS             22424..23473
                     /locus_tag="IXH60_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1989576.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            23486..24490
                     /locus_tag="IXH60_000612"
     CDS             23486..24490
                     /locus_tag="IXH60_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1989577.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            25123..26142
                     /locus_tag="IXH60_000613"
     CDS             25123..26142
                     /locus_tag="IXH60_000613"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1989578.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGNDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            26185..27066
                     /gene="galU"
                     /locus_tag="IXH60_000614"
     CDS             26185..27066
                     /gene="galU"
                     /locus_tag="IXH60_000614"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1989579.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            27366..27743
                     /locus_tag="IXH60_000615"
     CDS             27366..27743
                     /locus_tag="IXH60_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989580.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT         9119 a         4863 c         5715 g         8235 t
ORIGIN      
        1 ttttttattc agaaagttta cgaatacatc aaaattctga aacaaaaaac gtaactcaca
       61 aagagttgtt tttgcgtttt ttcttgaatg attaggtctg aatcttttgc tcacaacctt
      121 attcatggtg ccccggcttg agctgataga tttttgaagg acggccgatt cctacaggac
      181 gttcaccgat ctcatcgaaa tattggagca tagctttctt aaaattggaa tgatcgatcg
      241 ttttataatc gatccctaag aatttagcaa atacttttcg agcttccgta atcgtgaaat
      301 ctttacctaa aacttgtaga acttgcggct cgtgttccat tttattgact acacggttga
      361 atgctttcaa gatgatttga ctatggtcaa aagccaaagt atctttgcca gaagactctc
      421 cagtgactaa atcaagcgag atagccactt ctccgctaga taaatggatc ttgttactat
      481 gacgttctag tgtgaaccag cgtacttcct tggcatcatc tccggcgatt agcggttctt
      541 ctccaataaa ggctaagtag ctgactgtga cgacccagcc tcgcggatct cgattaggcg
      601 tactgaatgt atgcagttgc tcaatatttt cttttgaaat aaccacacca gtttcttcct
      661 ttgtttctct taagacgctt tctccagttg actcatcctt ttggacaaag ccaccaggca
      721 atgcccatga atttcgataa ggatgccctt ttctttggat caacaaaact ttcagctgat
      781 cttcttcttt gttatagcac atcagtacga tatcgacagt caaagagggc ttgtcatatt
      841 ctggacgttc ctgtttttga taccagttaa gaaattcttc ttcacttgct ttatgttcat
      901 aatataattt ttcttcttct ttagaagcaa aacggttcat ctgaccacct ccattgacag
      961 taaaagtact ttttacctta ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa
     1021 aaatctaggt tatcatttaa atatgaaaaa taaatggaag tatgagattg aataaaaaga
     1081 aaagcagata aaacggcaat actttatacc gctcttcacg aatggcaaag aagccacaaa
     1141 taacgatata tacgtcaatg ataaaacgca aaatccaagc tgctgtcata aaactgttca
     1201 aatgcccatg gaggttaaaa taaccataca tacgaatata aaaatagctg aatattaaat
     1261 gtgctaatga aagaaaagca atactaagtg taaaaaaaag atgtttcttc tgcatgattg
     1321 ttccagtccc ttactaagat agatagtctt tattatacca cgtgtgaggg gagaaataaa
     1381 aagccgtttt ttagtagata tgaaacaaaa caaaaacccg catttattca acctattcat
     1441 ccttttaaag gatagattga acgtagatag aagtcgttta tccagtcttt ttcattttga
     1501 aaaaaagcta acagcatgct ttttttatgg tatactagca aggaaacgaa ttcgcatcat
     1561 gcgataaagg aggaagagcc ccgtggcaga aaaggaaact ttttatatca ctacgccaat
     1621 ctattatcca agcggcaaac ttcatatcgg caactcgtat actacgattg catgtgacgc
     1681 aattgctcgt tataaacggt tgatgggctt tgatgtgttt tatctcactg gagtggatga
     1741 acatggtcaa aaaatcgaaa caaaagcaga agaactaggt gtaaaaccgc aagaatatgt
     1801 agataaaatg gcggctgatg tcaagaaatt atggaaaacg cttgatatca gttatgataa
     1861 attcattcgc acgacagatg attaccataa agcagctgtt caaaaaatat ttgatcgttt
     1921 attagaacag ggagatattt acttaggtga atatgaaggc tggtactctg tttctgacga
     1981 agaattcttt acagaaacac aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat
     2041 cggtggtaaa gcgccaagtg gacacgaagt agaattggta aaagaagaat catacttctt
     2101 ccgcatgagc aaatacgcag accgtctatt ggaatactat gaagaacatc ccgaattcat
     2161 ccagccagaa tctcgtaaaa acgaaatgat caacaacttt atcaaaccag gattagagga
     2221 tcttgcagtt tcacgaacaa cttttacttg gggagtacca gtcaagagtg atccgaaaca
     2281 tgtggtgtat gtatggatcg atgcattatc caattatatt actgcactag gatatggctc
     2341 cgaagatgaa agtctattcg aaaaatactg gcctgcagat gttcagatgg tcggtaaaga
     2401 aatcgttcgc tttcatacga tctattggcc aatcatgctg atggcccttg atttgcctct
     2461 acctaaaaaa gtatttgggc atggctggct attgatgaaa gacggaaaaa tgtctaaatc
     2521 aaaaggaaat gtcgtttatc ctgaaatgct tgtggaacgt tatggtttag atgcactccg
     2581 ttattatttg cttcgtgctg ttccgtttgg gtcagatggt gtctttacgc cagaagattt
     2641 tgtttcccgt gtcaattatg atctggcaaa tgatctaggg aacttattga atcgaacgat
     2701 tgctatgatc aacaaatatt gtgatggact cgttcctgac tatgcctcac ttgtcactcc
     2761 atttgatagt gaactgtcta cgacggctgc taacgttgtc ggacgttacc atgatgcaat
     2821 ggaaaaaatg gaattcaata cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa
     2881 caaatacatt gatgaaacag aaccatgggt attagcaaaa gacgaagaaa agaaagctga
     2941 attgaatagt gtcatgatcc atttagctga atctttgcgt atcgcagcga tattacttca
     3001 gccaatcatg acagaaacgc cagagaagat cttcaatcaa ctaggattgg actctgaaac
     3061 aatgaatctt gaaggattac actttggcga atttccttca ggaacaaaag tagttgcaaa
     3121 aggtacacca atcttcccac gtttagatat ggaagaagaa gttgctttta ttcaagaaaa
     3181 aatgtctgaa ggtactcaaa caaacgaaga tacagtcaaa tgggatccag aagaaacaga
     3241 acttgtttca acgaaagaaa aacaaatcaa atttgatgta tttgaaaaag tagaactaaa
     3301 agtagcagaa gtcatcaact gtcaaaaagt agaaggggca gataagttgc tgcaattccg
     3361 actagatgct ggagacagcc aagaccgtca aatcttgtca ggaatcgctg aattttatcc
     3421 tgatccaagc gaattgatcg ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa
     3481 gatgcgtgga caaatcagtc aaggaatgat cctttctgca gaagcaccag atggctcttt
     3541 acaagtaatc gaagcaccaa aatccatgcc aaatggctca gaaatcgcat agaaattcaa
     3601 agggggtatg acactagttg tgtcataccc cctttttccg aaatttgaga agcaattttc
     3661 gtaaattcct tcttattact tgaagccaat catataatac agaaacagag aactatccaa
     3721 aataaatcct agaaaaagaa gtacgagctt cgctgatttt tgttaattta gaataatact
     3781 aataggaatc gtaaaaacag aaaaaaagca taatgataaa gggtcgggca gattttaatt
     3841 gattcatctg gtactctttt ttattgtcct tacattcata aagtttgaat gcaatgactt
     3901 tattacatct atatttatat ctaacagtag cgttgatgtg ttaattataa gttctatcac
     3961 tttatgttca attagtttgt gctcttatat gtgtgattga aaatatagat aaaaaaacaa
     4021 tcatcggctt tgacttcgat gattgttttc tttattttgt aaaaatccta tcacttgatt
     4081 ttatggtaac cttttatttt tgtcttcaaa cggcttggta agagagcagt aagcagtgca
     4141 aatgggatcg aaaagacata ccagaaaatc gtacgtccga taccataatg ttgatagagc
     4201 tttgtcaaca tcacgtgtaa caagatcaac cctaaaagca aggagccagc tataaaccaa
     4261 tgattatccc aatgcatgac ttcactaagt gccatcactc caaaaggata gaaataaaaa
     4321 tagatcccga ctaaggtcat ccgccagttc tttgtaaatg ttccgatatt caataaaaag
     4381 aaaatcgctg aaagcaaaat accgataggc ggaaaaagcc caaccaaaaa ggaatacaac
     4441 atcccccaca agatcatcac aggacctttg atcaatgcaa cgattgcgat gaacaaagtc
     4501 agcaatatta gtttttgtga taacgttgca acagtcatta atccaaataa aattaatgtg
     4561 aaccaatgcc aatgggactg ctgttgttct ttacgggaaa aatcgacccg gttggataaa
     4621 taatctgcta atttcggata agaacgctta aaatccataa actgttcctc cttcgtggaa
     4681 aaggtaggtt tagtatacga aaaataaaaa agaaatacat ccgtcttaag agggagtttt
     4741 tcactgaaag tggagaaaaa agaatggtaa attaaaatat tctcagttat actgaaaatt
     4801 ataccaaatt tcaaagaaga aagaggatgg gaatgaaagg caaactaaaa cgagaaatca
     4861 atctgtttgg cgcattagct accgttatgg gaaccgtgat cggtgcaggc gtcttcttca
     4921 aaactgctgc tgttactgct agtactcagt ctacaagttt gacattgcta gcttggcttt
     4981 tgggtggttt cttaacgatc tgtgctggat tgacagtggc tgaattagct acagcaatcc
     5041 cggaaactgg tggagctgta aaatatattg aagctgcgta tggaaaattg cccagctttt
     5101 tattaggatg ggcgcaaagc ttgatttact ttcctgctaa tattgctgca ttatcgatta
     5161 tctttgcgac acaattgacg aacttattac aattatctac cgattatctt ttgttgattg
     5221 cagtgatcac cgctgtttct gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt
     5281 ctgtacaatc agtgacgttg attgttaaat taataccaat tgctgtgatt gtcatatggg
     5341 gattattgac cccaggacaa ggaactgtcc agttatttcc aatcgaagct ggaaaagatg
     5401 ttacttttgt cgaaggattg agcagtgcgt tgttagcaac gttgtttgct tatgacggct
     5461 ggttaggtgt cggtgcaatg gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag
     5521 caattattct tggtttgagt tttgtaacag tcgtttatct attgatcaat tttgtttttt
     5581 tgaaaacttt gccgattgat catcttgccg gtaatttgaa tgcagcttct gaagcatcag
     5641 acgtcatttt tggaggaatc ggtggaaagc tggtcactat tgggatcttg atctcggtat
     5701 acggtgcatt gaacggttat acactaactg ggatccgcgt gccttatgcg atggctttag
     5761 aagacgagct acctttcagt aaacagctga caaagctttc aaagaagttt acggttcctt
     5821 atgttcctgc ggttttccag ttagcagtag cttgtattat gatgagcctt ggttctttcg
     5881 actttttaac agacatgttg atttttgtga tgtggctatt cagtttactg atttttatcg
     5941 gtgtctttat tttaagaaaa aaagcgccgg aattgccacg tccatataaa gtaccacttt
     6001 atccaatcgt tccgattatt gcgattctcg gtgctatttt cattttaggt atgacgatga
     6061 tgacgcaaac gaaattagca ttgatcggta taggtgttac cttgataggt atcccagtct
     6121 attatcaaaa aaagaaaaaa cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa
     6181 gtacgaacta ggtcctctag atcttcgtgc tttttctatt tcaataaaac aagttcttct
     6241 attttgattt ttgctacttc tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga
     6301 gagataaaca tgatatttga ttctcatacc catttaaatg ctgaacagtt caatgaagat
     6361 attccagaaa cgattcagcg agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc
     6421 ttcgatacgc caacgattga aaagtctttg caattaagcc atgactatcc aaatatatac
     6481 agtattatcg gctggcatcc gacagaggct ggcagttata cgaaagaaat cgaaaataaa
     6541 ttacaagaac aattgacttt accaaaggtt gtagcattag gggaaattgg cttggattat
     6601 cattggatgg aagacccgaa agaagtccaa gataaagtat ttcgcagaca gattgctatc
     6661 gcaaaagaaa tgaacctacc aatcagtatt catactagag aagcacttga agacacgtat
     6721 caaatcctaa aagaagaaga tgttcgagat atcggtggaa tcatgcatag tttcagtggt
     6781 gattatgaat gggcaaaacg atttcttgac ttagggatgc atatttcatt tagcggtgtc
     6841 gtcactttca aaaaagcatt agacgtccaa gaggctgcag caaatgtacc aatcgaacga
     6901 ttacttgtgg aaacagatgc cccttatctg gcaccagttc cttttagagg aaagcgaaat
     6961 gaaccaggct atactcgtta tacagtagaa aagattgctg aacttcggaa tcttccgaca
     7021 gaagaagtgg ctgcccaaac atggacaaat gcgcaccgat tatttggtct aagcgaacat
     7081 ggataagatg aagatccaag agattatagt cgttgaagga aaagacgata caagaaggct
     7141 gcgggaagta gttgatgcag atacgataga aacaataggg tctgcaatca atgaagagat
     7201 cctgatgcaa attgaacatg cccaagagac tcgtggagtg atcgttttta ccgatcctga
     7261 tttttcagga gaaaaaataa gaaaaatcat tatggaagct gttcctcagg caaaacatgc
     7321 ttttttgccg cgcacacaag ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc
     7381 aagtgatgct gctattttag aagcattgaa aaaggtcgtg acgccagaca tttcccaaac
     7441 ggctgtttct gaaatcacta gacaagacct tttagactat ggtttaatag ctggggcaca
     7501 agcaaaagaa aaacgagaaa aactaggtga cgagttgcgc atcggttaca cgaacggcaa
     7561 acaattggaa aaacgtttga agatgtttcg tattacgccg aaagaactcg cccaagcaat
     7621 gaaaatagtg gaggaacaac atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa
     7681 aagagatttt attgaaacat ggtttttcat ttaaaaaaag tttaggacaa aattttctga
     7741 ctgaaccaaa tattttaagg aaaatcgtag aaactgcgca aatagatgaa catacgaacg
     7801 tgatcgaagt tggaccagga atcggtgcat tgacggaaca attagccaag catgcaaaac
     7861 aagtcgtagc atttgagatc gatgatcgtt tgatccctgt attggctgat acgatgcaac
     7921 cctatgacaa tgtgactatt gtacatcaag atattttaaa gaccgatttg tcaacagctg
     7981 ttagagaaac atttcatgag gagcttccgt taaaagtcgt agcgaattta ccttactata
     8041 ttacgacacc gatcatgatg cattttcttg aatcagattt ggttgtggat gaactagtcg
     8101 tcatgatgca aaaagaagta gctgatcgta tctctgctga accaggaacg aaagcttatg
     8161 gttcattatc gattgctgtt caatattata tggaagccag tttagcattc atcgtaccaa
     8221 aaacagtatt tgtccctcag ccaaatgtag attcagcgat tttgaaactg acaagaagag
     8281 atataccggc agtcgaagtg acagacgaaa aagcattttt ccgattgaca aaagcggcat
     8341 ttcagcaaag aagaaaaacg ctttggaata acttgcagca ttcgtatggc aaagatgacc
     8401 agacaaaagc atggttagca aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg
     8461 gggagacttt gtcattacaa gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc
     8521 agtaaaaaaa gaggttgtga cagaagttgt gaaaaagctg tcctgcatca agtaataaga
     8581 acagccaaac aaaaatagtt cctcatgttt ttcgtttgtt tgggcttatt accgcagatg
     8641 caagcttttg aacaccgttt aagaaagagg ttgtgggcct gccgtttaac tctaagtcac
     8701 aaggaacaat ttcaaaaaac agcttctcat gtttttgagt tagttggtct tattacttga
     8761 tgcaagcttt tgaccactat tatgccgaac aaggagatgt gacacaaatt ttgtcacatc
     8821 tcctattttt gtttcttgat caaaaaattc ttatacttga tactcacact cattttgtat
     8881 ggatttagaa aagaatatgt gtcttgacag ttattagtaa gtgagttata ctaagtcgac
     8941 atttatgtgt caagacatat taggagagag aaaaaatgag aaaaaattca gttcgaaaac
     9001 taacgatttc tgctttattg attgggatgg gggtcattat cccaatggtc atgcctaaaa
     9061 tcatgattcc accagcttcc tttactttgg cgtctcatgt gccattattt attgcgatgt
     9121 tttttacacc aggagttgca gtggctgttg cgctaggtac gacatttggt ttctttttaa
     9181 caacgccggt tatcatcgca ttgcgagcat tgtcacatct tgtgtttgcg ttgattgggg
     9241 cttggtactt gcaaaagcat ccgagtatcg ttttaaaaaa tggacagttt acttttgcta
     9301 attggcgttt ccaagggttc aattttgtga tcggtgtgat tcattcttta gcagaaatgc
     9361 tagtagtcag catcttttac tttattggta atatgcctga aacatattat tctcaaggat
     9421 atttttatac agtattcatc ttgatgggga ttggtgggct tatccacagt ttagttgatt
     9481 ataatattgc ttattttata gcaggaacac tcagcaaata ttttgatatc cccgtattta
     9541 cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg gtacgcgaag aaaacgacat
     9601 attagacatg atctctcaaa aaaaaaactc tcatctagta atggataata aatcctctaa
     9661 gatgagagtt ttttgtccta aaaagagctg caccaacatc ttctggcaca gctctaacag
     9721 ggggcaattt atggaagttt gatgacttgt tctgtttctt tatcaaagaa gtggcctttg
     9781 ctgatgttga aggcaaggtc gactatttcg cctggtttat ggaaatctct tgcatctact
     9841 tttgagatga actcagtaga accagttttt gtgtaaagca tcgtttcagc tccaagtaat
     9901 tcggaaacga caacttctga tttcactacg gcttcaggtg aagcttcaag ggctacttgt
     9961 tcactgtgga tgtcttctgg acgaataccg aagataactt cttttccttc ataacctttt
    10021 tcaactaata ctttgttttt tccttcagga atacgaagct taagcccatg tccatctgtg
    10081 ataacaccat ttttcaatgt cacattaaag aagttcatgg caggagatcc aataaagccg
    10141 gcaacgaaca tattgacggg tgtatcataa acttctttcg gtgtaccgat ttgttggata
    10201 aatccgtctt tcatgataac gattcggtca gccatagtca ttgcttctgt ctgatcgtga
    10261 gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg
    10321 gctacacgta atttagcatc taaatttgat aatggctcat ccattaagaa gacttttgca
    10381 tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt gtccaccaga taaagcagct
    10441 ggttttcgtt tcaagtattc tgtcagacct aagatctctg ctgcattgtc cacacgtttt
    10501 ttgatgtctg ctttatcata tttacgtaat ttcaagccaa aagccatatt atcaaaaaca
    10561 gtcatatgag gatacaatgc gtagttttgg aaaaccatgg caatatcacg gtctttaggt
    10621 gccacgtcat tcatgacttt tccgccgata tttagttctc cttcagagat atcttccagt
    10681 cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg aaggaccaac aaaaacgata
    10741 aattcacggt ctttgattga tagattaaaa tcagttacag aataattttc tgcattgtca
    10801 tattttttat atacgttttt cagagccatt tctaccataa tacgttccac actttctttt
    10861 ttagttttat taactattta aagtataaat gaaagcgtta actaacttca atgtcatgtt
    10921 gaacaagaat tttttctttt ttttgtcaat gtgctagttt taagctttaa taaatatcca
    10981 taagaaataa aaactgatac actggagtaa ataagtatgt taacaatatc aaaaggagtg
    11041 tctcttatga aaatcgcttt gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta
    11101 gcaacagcgt ataaaacaat attggaaaaa catgaacttt ttgctaccgg gacgactggg
    11161 cagaggatca cggaagcaac cggcttgccg atccatcggt ttaaatccgg accgttaggc
    11221 ggggatcagc aaattggagc catgatttcg gaggatcagt tagatttggt gatttttctt
    11281 cgagacccgc tggctgcaca gccccatgaa ccagatgtaa cagcacttat ccgtttatct
    11341 gatgtctatg agattccgtt agctacgaat atcggaacag ctgaggtgct actaagagga
    11401 ctggaagccg gcttcctaga ttttcgaaga atcgtccatg aaatagacaa gcatccactt
    11461 tcattttgat ggataagcgt atggaaaaat aacgtttaat gttcgcctat atgtagctat
    11521 aaaagctaaa caaactgata atatgcgata taatacgaac gtattggata atataaacaa
    11581 taatattagt ttttcgtatt tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa
    11641 agatcccaaa aataatacag agtagaaaat aaagcgttaa gtgctaagag gatgggatgt
    11701 tgcctcttgg acgaaggacg taatgttcgc ggttttattt tcgcattcgc tgcatgatac
    11761 gatgtagcag ctcttaaagg agatgctaaa aatgaagaaa caccggttag acgcacgtat
    11821 gattgctatt atgggcttat tgattgcatt gatggtcact ttgtcccgac tggtagcaat
    11881 cgagacccca tttattaaaa tcagtgtcac atttattcca caagtgatca tgggcattct
    11941 ttttggtcct ttttggtcag ggataggcgc agttttagct gatttagtag gaatggccct
    12001 cttctcgaaa tctgcattct ttattggatt tacattgaat gcatttattg aaggagcaat
    12061 ctacggattc tttttctatc gaaaagagat cacttggaaa aatgccattc tggctacgct
    12121 tagtgtcacg ctgatcatca acttattttt gacaccatta tggcttgcgt tgatgtacca
    12181 tgtgccattg ttcagctggg tcgtttgggc gccccgcctg ttaaaaacag taatatggtt
    12241 accgattcaa tcaattgcca tttattacgt aggacgtagt attccttaca aaaaaaattt
    12301 taagaagttt agcgatccac gctaaataaa aaacaagcag tcttcccatc ccagaagact
    12361 gcttgttttt attgtgctgt gaattgtgct tgatagagcc gtcggtaata accatctttt
    12421 tgaagcagct catcatgggt gcccatttcg acaatagctc cctgatccat cactaatatc
    12481 tggtccgcat ttttgattgt ggacaggcga tgggcaatca caaaactggt ttttccttcc
    12541 atcattgcta agaaggcttg ttggattttt ttctctgtca atgtatcgac tgagctggta
    12601 gcttcatcta agatcaacat agggggatta ctgatcatcg ttcgagcaat cgtcaacagt
    12661 tggcgttgtc catctgatat ctttaaccct tgaccgccaa tcttagtatc taatttttcc
    12721 ggcaaacgtt ctacaaattc atacatataa gaggctttta gggcatcatt gatttctgcg
    12781 tcacttgctt ccggattccc aaaacgaaga ttttctcgca atgtactgtc aaatagccaa
    12841 gtatcttgta acaccattcc aaaactttta cgtaatgtat cacgttggat agagcggatg
    12901 tcatggtcat cgactgtaat tgttcctttt gttgtgtcat aaaaacgcat cagcaaatta
    12961 acaagtgtgg attttccggc ccctgttttt cctactatag caatggtttg acctggataa
    13021 gcagtaaaat tgaaatcttc gatcaaacgc tgcttggggt cataagaaaa agaaacatga
    13081 tcaaaagaga cttttccttt gatatttttg ggttccaagg cataggcatc tggcaaatca
    13141 ggcttttctt ctggctgatc gatcaaatca aatgctcgtt ctagaccaga aaaagcggtt
    13201 tgcagttgtg tcgtgattcc agaaagttcg ataaatggtt tagaaaactg acttgagtag
    13261 atcgtaaagc tggaaatcac accaacagtg attgacgaac cactcttcaa tgccaatagg
    13321 ccgccaacta aaccaatcga taaataagca aggtgatcta caaaacgaga agacgggttt
    13381 gtcaaggaag aagaaaattg tgcccgttgt cctttttcat atagatcagc atttagcagt
    13441 tcaaattgct gttgactagc ttcttcttgc tgaaaagctt tcacgatttt ctggttaccg
    13501 attcgttcgg tcacaaagcc cgagatatcc ccaataatcc tttgctgtct atcgaaattt
    13561 gtttgtgaag cacgagcaac gatccaactc acaaggaaaa taattggtgt agaacaaaga
    13621 acgaccaatg tcagaagtgg acttagacgc atcataaaaa taaacgaaag aatgatgatt
    13681 gccactccag aaaataattg attgaaggcc gcagaaattg caatggaaat attgtccata
    13741 tcatttgtaa atcgactgac gatatttccg tgggagtttt gatcataata acgaagtggc
    13801 aatcgattca gatgagcaaa tgcatctttt cttaattttg caacggataa gtaagctaac
    13861 cgattgctta acacttggat gagccattga ctgactacgt tgattaaaac gataccaccg
    13921 aatagaataa gtaaatggaa cagtcgattg aagcggacct gtccttctcc aatcatttga
    13981 tcgatacttt gaccaatttg taacgtcatc acaactgttg caatcccatt gatgattcct
    14041 agtatcacag ctagccaaat gtcttttgca taggcagtaa ggtaaggaat gaatcggcgg
    14101 agcgtctgca aggataattt ttttactttc ataactattc ttcctcctgt gatgcgacga
    14161 tttcctgata ttctgctgat tgagcaagta attcttcatg ggtaccctga gcagcaatct
    14221 taccttgatc catcactaag atatgatgtg cttcttgaat agagcggaca cgttgggaga
    14281 taataatcac tgtactgcca agataagtct gtagcgcctt acgcaaatcc aagtctgttt
    14341 gataatccaa cgcacttaaa gagtcatcta aaatcagtaa tgctggcttg gcaatcaaag
    14401 ctcttgcaat cgtcagtcgc tgtttttgtc cgccagagaa atttttccca ttttccatga
    14461 cttttgtatc taaaccattt ggaagctgac ggacaaattc ttctgcctga gcgattttca
    14521 gagcttccca acattctttt tctgtggcgt ctttcttacc ccattgaagg ttttcacgaa
    14581 tagtcccggt aaatagtaca gaagtttggg gaacaagagc aatttgctga cggagatgat
    14641 ccagctgcca agaacgaaca tccattccgt ccattataac tgtaccggaa ctgacatcat
    14701 agaaacgcgg aatcaattgt gtcaaagtcg ttttcccgct tccggttgga ccaatgattc
    14761 caaggactgt atttcgcttc agctgaaatg taatattttg gagagaaaga ccagaatcag
    14821 gagtataacg gaagtcaaca tggtcaaaag cgataattgc ttcagaagaa aaatcgggag
    14881 tagtactgac ctcagcttca agaatcgaat tttctgtgtc tagcacctct ttcacccgat
    14941 ttcctgaagc ttcggcacgt gtgaagatca cgacgaggtt tgaaacaacg atcagtgcca
    15001 aaagcatttg attcatataa ttgatcaaag caagaacttg tccttgttgc aagctaccaa
    15061 tatttacttt gatcccgcca actgtcagca aacagatgat cccgacattc atgatcaagg
    15121 ttgtagcagg tgataatagg gcagagatat tggaaacacg taaataattg ttcgctagat
    15181 catttgtgct tttatcgaac ttctctgttt ctttttttgt ctgcgcaaat gcgcggatga
    15241 cacgaacacc gctaagattc tgactaacgt gttcattgag ctgatctagt ttttcttgca
    15301 ctttttggta gagaggaacg gtttttttga tgataaaaaa taaaatgatt gagaaaatag
    15361 gcaataatgc taagaaaaac agacctactt cccagtcgat cacaaaagcc atgaccactg
    15421 acccgatact taaaaacggt gctctgatca ataaacgaat gaccattgct aaagctagct
    15481 gaagctgatt gatgtcgttt gtcattcggg tgatcaaggt atctgttcca aaatgattca
    15541 actcgttgtg agagaaagta ttgatttttt tgattagctg attacgtaac tctgtaccaa
    15601 atccttgtga agctactgaa gcatagtatt ggcaaatgat agcacaaatc agaccaatca
    15661 cagacatagc gaccatccat aagcccattt caatgatttt tcctcggtca ttcattttta
    15721 gtccattatc gatcaaggaa gccatcatta aaggcaggac aagttcgaag acagcttcaa
    15781 gaaatttgaa gaacgggccc aaaatgattt gcttacgata gtcttttgca taacgtaata
    15841 actgaatcat atgattcctc ctattattta tttgtccaca attgaaaaaa caagtactac
    15901 cacattgtag cttaggaaat ttagaatgaa aagagaacaa gccttttcat aaatgaaagt
    15961 tttattatga agaagcttta gaaattgaat agactatttt ttttaaaaaa aagaaaatga
    16021 gaaaacatgc cagtctcttc attcatagga acgtctattt tacttaattt ttctataaat
    16081 gaattctgca taaaaaatat atcaagtaaa tgtttataga tgaaactcta tttacttctt
    16141 ttttaatttg ttcttgcaat cttttttgct acgtaatttt tgtaaaaaag tgggttgaga
    16201 gaaatctaat tgattttcaa agaaggagta tgaaaatgag aaaaatattt attcgtttat
    16261 tgtgggtact atcttagagg aataaataat agtggagaaa actatggttt ggcgcagatt
    16321 ctatcaggtt tgatggaacg aagacaacct cccgacatca agtcaaaagc tacttgagat
    16381 tttgatctga aaataaaaaa agaaaacaag gtaggaacat caatgtttct accttgtttt
    16441 cgtaatcaat gggaaaaaac taagagtggt gtacgtaatc aataaaaagg atggtgacaa
    16501 cagtacctct ttactctctg aaagcatcat aactcgtttt tcctcattat gccaataaat
    16561 tgttgttata ccacatataa ggatttgtaa tagctaaaaa cagacaggaa agttgaaatc
    16621 tgtgaatctt attttgtatg gaaggattta tagaacgcaa aaatcaagat aaagatgaat
    16681 aaaaatcctg tttcggctat atattgcgta tctacgccaa taaaacgagt ttgttctgtc
    16741 atagccatat aaagcaaagg caaaaataca actacagcga gataagatcc gatgatttct
    16801 cgaaaagttt tccaggaagt atgtttcctg tttttatatt tatccgattt cattgaatag
    16861 tcatagcctc cttattcttt catatgttga aattccagta taaaaactgg aaggaactgt
    16921 acgtttttta gaataaaaat tataagggat aagtaggctg ctgtctataa taagatatat
    16981 ttttcaattc tttattaaag attttattga caaaatgaat ccgttttcta tgatggagga
    17041 ggggtgaata agttgttgaa gaaggaaatt ttatgttact tagagaacca gacagccttt
    17101 ttcgatttag aacatatgaa cgaggtcttt acagcacaaa ggttagctga tcattttggg
    17161 gtaaaaagaa acacagtcag tcattatttg aaccagttaa atgatgagca actactagtc
    17221 aaaatcaatt ctcgaccagt gatttatttc cacaaagaag cttttgaaaa acaatttttt
    17281 aagttacgca cgaattttta tttttctatc gatgatctca aagaggaaca gccttttttc
    17341 gcgcaaccaa aagacttatt ctcttttatg atcggatacg acgccagttt gaaagaaagt
    17401 atcgaacaaa tcaaaacagc tctttactat ccagatggcg gattaccact tttgattaca
    17461 ggcgaaagtg gcacaggtaa aagttattta gttaatttgg tctatcagta ttgtctgctt
    17521 catgatctgc tagaagattc tgcaccattc atcactttta attgtgcaca atatgcagat
    17581 aatcctgaac ttttgactag caatttgttt ggacatgtta agggagcgta cactggtgca
    17641 gaagaaaata aaaaaggggc ttttgaagca gcagatggtg ggatcttgtt tttagacgag
    17701 gtacatcgct taacaccaga aggtcaggaa aaactgttca cctatcttga tcaaggtgtg
    17761 atttatcgta tgggagaacc taatttgtca cgccggatca aaacacgtct cttttttgct
    17821 acgactgaag agattactag tcatttctta acaactttca ttcgaagaat acctatccga
    17881 atcgaactac ctgcacttaa tcagcgtagt cgtaatgaac ggctagaact gatttactct
    17941 ttttttctcg aagaacaaag aaaaatgggg agccctttgt cagtcagtgg accagctttg
    18001 tcacttttaa caagtcagaa attatccgga aacataggag agctgaaaaa tatcgtcaaa
    18061 gtttcagcag caaaagcata tgctgaacag cgtgaacagc ctgaaatcca tgtcacgatc
    18121 catcatttgc aaaaagaact acttgcgcag cctattacta aaaatacagc acagcaagga
    18181 atctatatta cgcaagacca gaccttagag caattaatgg agaagcaaca gcccgaacag
    18241 cagcgaatcg ttaaaagttt tgagcaaatc ctaattgatt tcaagaaaaa taactgtctg
    18301 ctatattctt gcgaaggaaa attaaagcaa gaagtgattc aattatttga ttttttgctg
    18361 tttgagacca gtagacaaga aaaacacgag ctgctggttt attggacaca atctatccga
    18421 gagacctttc gacaaatgga aactgcctat caaatcaaat tcgatggaaa cagtgtctat
    18481 gccttgagtt actatctcta tcagcgaagc actatcaaat ggttaccgga agaacatgat
    18541 attatccaac taatcaaaga attagaacaa caagtttctc agtcttatcc atctagttat
    18601 cactatgtcc aacgaattct ggaattaagt aagatcaaat tagatattga agacacaagt
    18661 atggatcgga ttttactgac gatctatcta aaaaaagcga aatggtcaaa agaaaaacga
    18721 ctgccaaaag cgatgattgc tgctcacggt tatgcaactg ctagcagtat ggcaaatgta
    18781 gtaaatcgtt tattacaaga ggatctattt gaatcatttg atatgccact agatgtcaca
    18841 ccacagcaga ttgcttcaga aatccttgat tattgcgaaa acagcgatgt ttcaaatgga
    18901 ttgatcatat tagtagacat gggatctctg aaagaaatcc atcagttttt taaaaaacag
    18961 ttatccgtac cgcttttgat tttgaataat gtgacgacac ctttagcaat cacagtaggg
    19021 gaatgcctgc aaaaaaatgt atcattagaa gagatagcag aagagactgt tcgacagatc
    19081 cagccagaat ggcgactgct ctatccagag gaaaacaaac ccaaagcact cattactact
    19141 tgcttcactg gaatcggtac ggcggtccac ttaagtgaac tgctagaaaa aagcttacct
    19201 acggcttgtc agttgaaaat catcccatat gaatatcagc aattaaaaga taagaaaaac
    19261 agtgacccat tattttccat atacgaagtc gtaggaatga ttggcaccac agatcctgat
    19321 atcaccaata tcgactatct ttcattagaa gacctcatct cgggggaaaa gatgtctgta
    19381 ctagctgaat ggttggacac tactatgaat gcttcagaga aggaaatatt caatcagcgc
    19441 atcattcgta atttttcatt ggaaaaagta ctggattccg tgactattct agatacagag
    19501 aaggtgatgg gagaaatcga tcattttatg agagaattag aggtgcagtt gaatcgaagc
    19561 atcagcaatc ccaaaaaatt agcgttgtat gtccatgtga gctgtctcat tgaacgattg
    19621 atccgacaaa tgccaatcga aacctatcaa ggattagaga aatggaagca gtgtcaaaaa
    19681 gaagggttat ctgctattaa atctgcgttt agtgtcattg aagagcatta tagtgtcatg
    19741 atccccaatt ctgagattgc ctatatctat gatattttga gtgaagatac agagtttctc
    19801 tcgatagaag aagaattttg aacataagca agttgttgcc actaattttg gcatgcaact
    19861 tgctttttta gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga gaaattatct
    19921 tagcttccca tggaaaattt gcttcaggga tattagattc attagagctg atctatggga
    19981 aaaatcattc tattactgta ttcgattgct atactgatga gaattttgat ttagcggaaa
    20041 cagtaaggca gctttttatg aggtacaaag ataaagaaat cattgttctg acggatcttt
    20101 ttggcggaag tgtcaacaac gaattcttac aatatatcaa tcgggatcat gtctatctcg
    20161 ttgccggatt gaatttgcca ctattgattg agctagtatc tcggttggac gctgaagtag
    20221 agacaactgt tttgatccag caggtgctaa ctgattcaaa aaaaatgatc cagttttgta
    20281 acgagcggct caatcagaaa atagaagaag acgaatttta ggaggaagaa aaatgattgt
    20341 attgacaaga gtcgaccatc ggttattaca cggacaagta gctttttcat ggacacaaac
    20401 aataggcgca gactgtattt tgattgctaa tgatgatgta cctacgaatg agatacgaaa
    20461 gacaacgatc aaattagcca agccacaagg agtcaaacta gtgatcaaat caattgatga
    20521 ctcgattgcc gcgttgaaaa gcggggtgac agataaatat aagttattca tcgtagtgga
    20581 atcgatcgag gatgcatata agctggcaga agcgtatcca gaaatcaaaa ccatcaactt
    20641 aggagggatc aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa atattttacc
    20701 agaagaggag gaacgcttga aacgattaat tgacaaacaa atagaagtag aaatccgtca
    20761 agtaccagca gataaaaaag tggatgtaag agaagttttg taagaaagga agaacctcaa
    20821 tgattgtaca agcagtctta ttaggaattg ttgcatttgt cgcacaatca gaatatgcac
    20881 tagggacatc gttgctttct cgtccgattg tgacggggct gtttacagga atcgtgttag
    20941 gtgatgtaaa aactgggatc attatgggcg caactttaga gttagcattt atcggatcgt
    21001 tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta gggacggctt
    21061 ttgctattac agctggagcc ggtacagaaa ccgcgctttt actaggtttg ccgattgcta
    21121 cgttgacatt gatattgaaa aatatttact tagggctttt gattccaatt atgaaccata
    21181 aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg cacttgcttg
    21241 ccggtatcgg tctttccttg atgcttggtg ttgtggtaat ggtttcttat atggtgggaa
    21301 gcaatacgat cggaaatcta ttaaatatga ttcctgactt cgttcaaaag gggttgtcag
    21361 tagctacagg gttgattcca gctcttggtt ttgcgatgct tgctcgtttg ctgatcaaca
    21421 aacaagttgc accatatttt ttcttaggtt ttgctatagc ttcttactta gagattcctg
    21481 tgaccggagt agctattttt ggtgcgattt tagcagttgt ggtagtcaat attatgaatg
    21541 tccgtcaact accacttcaa acaactgcag gggaggtaga agaagatgaa gaattctgag
    21601 atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg tattttggcg ttcttttcaa
    21661 atggagtttt cttggaatta tgaacgtcag atgaatatgg cttatgttta tgcaatgatt
    21721 ccgattttga aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt gaaacggcat
    21781 ctagagtttt ttaacacgac cccacatatc gttacactga ttttaggaat cacggctgca
    21841 atggaggaag aaaataagga tgatcctgaa tttgatgtga ctgcgatcga tagtatcaaa
    21901 acatctttga tgggacctct tgcagggtta ggagattctt tcttttgggg aactttgcga
    21961 ttgattgcca caggtgtggg tacctcttta gccatgcaag gaaatatttt aggccctatc
    22021 ttgttcattt tgattttcaa tattccgcac attctgtttc gctatctagc aacaggttgg
    22081 ggatatcgct taggaacggg ttttttgaaa aagatccagg caaacggtat gatggaaagt
    22141 ttgacattag gcgcgtctat tattggactg atggtcgttg gagcaatgac tgccacgatg
    22201 atcaccatca atattccttt aaaaatcggt tcaggagaaa atgctgtgac tgttcaaagt
    22261 atttttgatg atatcgttcc aaatatctta tgtcttggag catttggcat agtgttttat
    22321 ctattgaaga aagaagtgaa accattaacg atcttgatcg gactagcttt ctttggaatt
    22381 tttggttcgt ggatagggat tttttaaaaa aggagcagag aaaatgttta cgatgcaaga
    22441 ttatatctat gaagaaaagg aagtactaag tacgatcttg aaaaagaatg atttttcgac
    22501 aagagaacat atgaaaaaaa cagtgaattt actgatctta gcaacgggtt catcttataa
    22561 cgcttgttta gcagcgaagc cagcattgga atcttatggt gatcttactg tcgatattca
    22621 agaacctttt tattttttca attatggaaa actttctcct tcaatcgata ctgtgattgc
    22681 tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga tacaaaaaca
    22741 agggttacct gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt tagaagctgc
    22801 acaaattatt gatttaggtg ccggggtaga atcagtaggc ttcgttacaa aaggctattc
    22861 tgcgactgtt ttacagttac tactcttagg gataggaatt ggaataagta aaaataaaat
    22921 cagcaaaaaa atagaacaag actacatgca acagttgcgc aaaataatca accatttacc
    22981 agcgattatt caaaaaacgg aagaattttt tgatggatat caaagtttat ttcgtttagc
    23041 acagcgtttc gtcaccattg gatatggtcc aaactgggga acagctaaag aagcagagac
    23101 gaaattgact gaaacaatcc gcgttccgtc acaaggattt gagttagaag cttatatgca
    23161 cggcccatac ctggaggcag atgcttcgca tttgctattc tttattgaag gggaaagtgt
    23221 aaataaggaa cgttcgcaaa agttgcaaag atatatgagt cggtacgttg gagagactct
    23281 caccattacc acaaaaaaag caagaaacga gaaaacactt ggattagcaa tagaatgtga
    23341 tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt ataaaaccgc
    23401 agtagctaaa ggtattgact tgaacaaaaa gatttttgaa gactttgata cagtcttgaa
    23461 aagtaaatta taagaggtgg aaatcatgct gaaattcaat gaagaagaac aaatcaaagc
    23521 aataaaaggc gcactagctt tacgtccgca agtagaagaa atcatcgata aactttatgc
    23581 ggaaaaattt gacgctgtct attatctagg aatcggtgga acgtatgctt cctcgatgca
    23641 agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc atgctgcaga
    23701 atattacact actggaaaca aaagattaac aaaagattct atcgttattt tgtcctctgt
    23761 taccggtaca actcaagaag tagtgaaagc agtagaacaa atcaagaaag taggagcgac
    23821 actgattgga tttatcgata aagcgaacag taagttgtct cagctatgcg attttgtggt
    23881 cacttatcca gctcctggaa cagaacaaat caaatttttt atggcagcag atcgattgat
    23941 gtatttgaat ggagaattcg aagcttattc agaatactat gaacagctcg aacgttattt
    24001 accaacaggg ttagtggaag cagagaaaaa agcagatgca tttggcttat catttgcaga
    24061 aaaacatcga cgtgatagta tgcattattt tattggagca gggaatcaat ggggagctgt
    24121 ttattcatat gccatgtgtt actgggaaga acaaagctgg cttccatcga aatctatcca
    24181 tgctgccgag tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc cagtcacatt
    24241 atttttaggc gaagatgaac agagagtgtt atctgaacgt gtggctaaac ttttgccaaa
    24301 aatttgcagc aattatacat tgatagacac aaaagactat ccagtagaag gtatcagtga
    24361 aaaatatcgc ggacgtgttt tgtctttctt attgatgcat gtagtgacac aacgcatcga
    24421 tgctcatgtg gagcagttga actgccatcc attagagata cgccgctact atcgtcagtt
    24481 tgattattag gatagactgg ataaaaagaa tataaaaaat aaagagagca gcgataactg
    24541 ctccctttat ttttttaaaa tattggcatc aattacgtta taaaacacat tttttgcatt
    24601 atctacagtt caacttataa tctgtttaat gggacatgtg ggtgccaacc attttttgtt
    24661 atgcaatagt actatttagt tgttgcatgg atccgtagta aaagtccaag tacaacgagt
    24721 tgcgccagtt tacatagcgt tttgtatcca gaatgttgac taaaacaaaa gttcctatgt
    24781 caaccgtcaa cagagacaat ctgtccgttg caggacctga cagatttaga aatgttcaat
    24841 ctgtctctta tgtattttaa atcaatactc actaggagaa gtttcctaac ctcctaatag
    24901 taatgctggt agtaacattg tcaaaactgc ttcctcttag gaacaagcaa agcataacga
    24961 gtgtaaaaat aaaatgtaga ctattgtaac atatcattga ttatttcaag taaaaacgat
    25021 aaaagaagta aacaaacact taaaaacaat ggaaattagt cccaacttta agaaatgtat
    25081 actataatgg aaattatcaa ttatttattt ggaggaatat ccatgaaaca aaaaatcgca
    25141 gttcttggtc ctggatcttg gggcactgct ttagcacaaa cattggcaga aaacgggaat
    25201 gatgtccgta tttggggaaa tgtacctgaa cagattgatg aaatcaataa gtatcataca
    25261 aatcagcatt ttttacctga tttgacgatc ccagaatcga ttatcggata taaagaattg
    25321 gcagaagcag tggataacgc agatgctatt ttatttgttg ttccaacaaa agcgattcgt
    25381 tcagtcgcac aggaacttat tggtaaaatg aatactaagc caatcattat acatgcaagt
    25441 aaaggactag aacaagatac gcataaacga atctcagaag ttttggctga agaaattcca
    25501 gaagaaaaaa gacaggcaat tgttgtatta tctggtccta gccatgctga ggaagtggca
    25561 gtacatgata tcacaacaat tacagctgca agtattgatc aaaaagcagc agcctatgta
    25621 caaaaactgt tcatgaatga ttatttcagg atttatacaa ataatgatgt gattggcgta
    25681 gaaactggtg cagcactgaa aaatatcatt gcaattggtg ccggcgctat ccatggatta
    25741 ggattcggag atgatgcaaa agcagcaatc atgactcgag gattggcaga aatcagccgt
    25801 cttggtgtcg cgatgggtgc caatcctctg acatttatcg gtttgagtgg tgtgggggac
    25861 ttgatcgtta cttgtacaag tgttcattct cgcaattggc gtgccggtaa cttgttagga
    25921 cagggacata agctggaaga agttctagaa aacatgggaa tggtcgttga gggtgtggca
    25981 acgacaaaag cagcagtaga gctagctcaa cagctaggtg ttgagatgcc aattactcaa
    26041 acaatctata atgttttgta taatggagaa gatatcaaaa aagcagcaaa agaaatcatg
    26101 ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt aaatagatga tcaatagtta
    26161 atcgtcgttt aggaggaaca gcaagtgaag gtcaaaaaag cagtcattcc tgccgctggg
    26221 ctaggaacac gttttttgcc ggcaacaaaa gccatggcaa aagaaatgct tccaatcgtg
    26281 gataaaccaa cgatccagtt tattgtggaa gaagcattgg attcgggaat cgaagacatc
    26341 ttgatcgtaa cgggaaaaga aaaaagaccc attgaagatc actttgatgc aaatatcgaa
    26401 ttggaaaaca atttgcggga aaaggaaaaa acagaattac tcgctttagt tgaagaaacg
    26461 actcaagtca atcttcactt catccgtcaa tctcatccaa gaggactggg ggatgccgtg
    26521 cttcaagcaa aagcatttat aggaaatgaa ccgtttgtcg tcatgctggg ggatgatttg
    26581 atgaaagatg atatcccatt gactcaacaa ttgatgatgg attatgaaga aacacaagct
    26641 tccgctgttg ccgtcatgcg tgtaccagaa aatgaaacat ctaaatacgg aattattgat
    26701 cctaaaacag atagcgaaaa gggtcgttgc agagtaaaag ggtttgtaga aaaacctgaa
    26761 atgggaaaag caccaagtaa tttagccatt atcggacgtt atctactgac tcctaaaata
    26821 tttgagatat tagaaactca agaaccagga gcaggaaacg agatacagct aacggatgca
    26881 cttcagactc ttaaccagac agaggcggtc tatgcgagag agtttaaagg aaaaagatac
    26941 gacgtaggcg ataagttagg atatatgaag acaaatattg aatatggact tcaacattcg
    27001 gaaattggga cttcattaag tgcatatatt atccagttga gtaaaaagct aagaacagaa
    27061 agttaactgg aaataagtgg cctgctgaga atgaaaggct atttgttatt aaagcggcag
    27121 cttgtgcgtc atttgcgcaa ctgtcgtttt agttttttga ctctagatca gttgagtcgt
    27181 ggaaaacgaa agatagaaaa agactttcgt gaatagattc agaaaatatt atgctaatgg
    27241 tttaattttt ttttaagttg tataaaagaa aatgattaca taagagaaat gaaagccaag
    27301 ccagaagatt catagtagaa taaaaaggcg aattttttaa ttataaaaaa aggtgtgtgt
    27361 tcactatggg aaaattagtc gctgttgcac agtcgctgct tgatatactc aagtggctga
    27421 tggaaggaag caattggata tttgtgttgc cactattgct tattagtcta tttatcatgt
    27481 ttcgcatggt cagaaaaaaa gaaacgtttc tgatttttat ctggtatgca gcatccgttg
    27541 gggttgctac cttgatggtt gaattggcgt taggtagttg ggaagcttgc gtatttgctt
    27601 tattttttgc tgtactgtat ctaatcattt atatcccata tatgaaaaga aagtctcgga
    27661 taggcagaaa aggtagacat acaactactt tttctagtac agattctctc gaacaaagta
    27721 gaagaacgag aagaagacgg taatatttag taggtatacg caaaaaaaga taaaataatg
    27781 gataaactat tgagatgaga gatttattta attggtggaa aaaagctttt ttcacttata
    27841 taaaagaatc ttttttaaag gaatgcttgt tgtatcggga taagccattc ctttttttat
    27901 gttatttata tatatataaa tcataccaat gt
//
LOCUS       DACEZN010000018        27634 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEZN010000018 DACEZN010000000
VERSION     DACEZN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.018"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(23..388)
                     /locus_tag="IXH60_000616"
     CDS             complement(23..388)
                     /locus_tag="IXH60_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989581.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYAVCIIKRDK"
     gene            complement(563..2698)
                     /locus_tag="IXH60_000617"
     CDS             complement(563..2698)
                     /locus_tag="IXH60_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1989582.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2781..4436)
                     /locus_tag="IXH60_000618"
     CDS             complement(2781..4436)
                     /locus_tag="IXH60_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1989583.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4438..4752)
                     /locus_tag="IXH60_000619"
     CDS             complement(4438..4752)
                     /locus_tag="IXH60_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1989584.1"
                     /translation="MNLLFQSIKSITVPELKEKILENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4922..5182
                     /locus_tag="IXH60_000620"
     CDS             4922..5182
                     /locus_tag="IXH60_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1989585.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5196..5510
                     /locus_tag="IXH60_000621"
     CDS             5196..5510
                     /locus_tag="IXH60_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1989586.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5995..6549)
                     /locus_tag="IXH60_000622"
     CDS             complement(5995..6549)
                     /locus_tag="IXH60_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1989587.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6675..8603)
                     /locus_tag="IXH60_000623"
     CDS             complement(6675..8603)
                     /locus_tag="IXH60_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1989588.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8909..9625)
                     /locus_tag="IXH60_000624"
     CDS             complement(8909..9625)
                     /locus_tag="IXH60_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1989589.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9626..11449)
                     /gene="glpO"
                     /locus_tag="IXH60_000625"
     CDS             complement(9626..11449)
                     /gene="glpO"
                     /locus_tag="IXH60_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1989590.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11463..12959)
                     /gene="glpK"
                     /locus_tag="IXH60_000626"
     CDS             complement(11463..12959)
                     /gene="glpK"
                     /locus_tag="IXH60_000626"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1989591.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13095..14555)
                     /locus_tag="IXH60_000627"
     CDS             complement(13095..14555)
                     /locus_tag="IXH60_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1989592.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14545..15840)
                     /locus_tag="IXH60_000628"
     CDS             complement(14545..15840)
                     /locus_tag="IXH60_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1989593.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16172..16849)
                     /locus_tag="IXH60_000629"
     CDS             complement(16172..16849)
                     /locus_tag="IXH60_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1989594.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16879..17970)
                     /locus_tag="IXH60_000630"
     CDS             complement(16879..17970)
                     /locus_tag="IXH60_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1989595.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18071..18505)
                     /locus_tag="IXH60_000631"
     CDS             complement(18071..18505)
                     /locus_tag="IXH60_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1989596.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19089..20546)
                     /locus_tag="IXH60_000632"
     CDS             complement(19089..20546)
                     /locus_tag="IXH60_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1989597.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20543..22438)
                     /locus_tag="IXH60_000633"
     CDS             complement(20543..22438)
                     /locus_tag="IXH60_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1989598.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22496..24271)
                     /locus_tag="IXH60_000634"
     CDS             complement(22496..24271)
                     /locus_tag="IXH60_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1989599.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24321..25817)
                     /locus_tag="IXH60_000635"
     CDS             complement(24321..25817)
                     /locus_tag="IXH60_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1989600.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(26011..27633)
                     /locus_tag="IXH60_000636"
     CDS             complement(26011..27633)
                     /locus_tag="IXH60_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1989601.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
BASE COUNT         7828 a         5951 c         4601 g         9254 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtattcaa
       61 aagatttatt tacttttttg tatagatttc gattgttcca cctagtcaat acaaatgagg
      121 taatcaggaa actgaaaaaa atcaatacaa aaaagccgat taagaaatac agcattttct
      181 tttctgataa aaaagcgtac aaggaaaaat ccttggctct ttctggtctc gttaatggga
      241 aaaagaccca tataaaagga agaacaacga tcatccatcc taaaagagcc actaacgttt
      301 gccaaatctt taaccagtaa ttaccttttg aaaaatactt atctttcacc aaaattgatg
      361 caacttcttc tcttgttact tttttcatat cctcacctca cgtttttttg ttacgacgta
      421 ggagtttacc atacatttca tttcttttca cataaagtgt tcctaaattt ttcctaagaa
      481 aaagaacaaa acatttctca tcagcaaatg aaaaatattt tgttcttttt atcagttgtt
      541 ctttttattt tttatctctt aattatgttg ctctttccaa tacgacaatg cttttcattc
      601 ctctagatat cgccgtgtac atttgtctgc gtcttctttg ctcatctcca tcgttttcaa
      661 atggatataa gattacatga tcaaattcca gtccttttgc tagactcaaa ggaatgattt
      721 ggcagttttc taatccatat gaattttttt taatcgattc ttgtagcgtt tgggtttcct
      781 cttctgtttg ggtgataact gccgcttttt ctccttttag agaaaccaaa atctcagcta
      841 gtattttcaa caattcttca gaggatcgaa aagagcggta ttcgggttct tttccttgcg
      901 gtcggatcga tacgatgtcg atcttctctt gatccacagc ataggttttg aaaagctctg
      961 tgattgctcc gctagaacgg taactatttc taagatcata ccgatggatc ggcagatggt
     1021 agtcatcgaa acaacgcata atatcagcaa aagtagtgga agaattaaag attgcctgat
     1081 tctcgtcacc tactaaagta aagtgagttt ttggaaataa ttcgatcaat agacctaatt
     1141 gtgccagggt ataatcttgt acttcatcaa tcaatagata gcgaaatgct ggtacattta
     1201 ccttttcaac aagtaaatga cgaatagcta atataatgac tgcttcatca acagtacgtg
     1261 tgggttgcca agcataagct gagccatatc gtttttcata aattctttca aacaactgat
     1321 gctcatccac ccatgccatt tcttcgattt gtctggttat ttttcgatat tttttttgta
     1381 aaagtttcct tgcataagct acaatacttt gttccgaatc atcagaaatc aatttaccga
     1441 ataattccaa ctgcctatct tctgtcaacg agagtacctg attctgcaca gcaggacttt
     1501 ttgcttgttt caaaagatga ttcttccatt cgctcatcag tgcctttttc gttgcttgaa
     1561 gcctatcgat caatttagga tgagcaggaa ctttctcata tattttttcg atcgtttttt
     1621 tagaaaggac tgtttttcca tttttagtaa gtccacggat aaaattcgga tgatctaaaa
     1681 acaaaggatc tgattgtttt agattatcga taaacttttt gctacgcaaa cgttctgtct
     1741 gctccgatac attttctccc gtgatacgtt tgaaataagc ctcttcacct tctatttcct
     1801 ctgctgaaag ctgtgaaacc agctgtatga tggttagatt cagtggattt ctttcaccta
     1861 atgatggtag gacattcgaa atatagtcga taaaccgatg attaggtgat agaatcagca
     1921 agtcatcgga tgtcatcgta tctcgatata aatagagcag atacgcaata cgctgcatga
     1981 tagcagaggt tttaccactt cctgctacgc catttaccaa gatattttga cttttcacat
     2041 cccgaatgat tgtgttttgt tccgattgga tcgtagcagt gatagccttc atttcattgg
     2101 tctcatcttg gcctaagact tctaataata cgtcatcttg aatagctaca gacgtatcaa
     2161 aaaacttgac caaacgatct ctttccaaaa gaaattgtcg tctttcttta ataaccgtat
     2221 cgattttatt ttgacgaacc gtataagaag atggaccaat catgttattg taaaaaagtt
     2281 cggcaattgg tgaacgccaa tcataaacaa gattttcatc tgcatcatct gtgaaatttg
     2341 ctgtaccgat ataaaaggat tcttttggct cattatccag aaagtctaca acgacttttc
     2401 caaagtaggg acttttcaat aaacgttcga tctttgctag ctgattttct gcgtttttct
     2461 gtttgatatt catctgatcg atttctctat ttttcatttc aatcatcgaa aaagtatcca
     2521 ggttatctaa gtaactgtca aaattcaatc gaagctcgcc agccatttct ttcaacgact
     2581 ggtttccttc tatttttgcc gtatccatcg attcccgcag ttcgtttttt ttatcaatta
     2641 gcttttgata cacgttcttc agatatgctt cttctaactg tttttgtgtg ttgaccatac
     2701 acattctcct ctttttttaa agtggtggaa ccgattagtt tacctctttt aagagtcgaa
     2761 tgcaagcttt ttaacgagta tcatttgtta ggatatatca gttcttcagg tcggacagtt
     2821 tgatataaag aaaatgcacc atcaaggttt tttacgtggt agccagactg cttcaagatt
     2881 ctttccgcta gatagctacg taatccgcta tgacagctga cgatatattc ttgttggctg
     2941 tctaattcat ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc ttccttgaat
     3001 ttgccctttt ccaattcttc tgctgttcgc acatctaata aaatttttcc ttttgctagt
     3061 tctgtcggca attcatgcca ttgtacggtt tcactaagcc cttctgcaat gttcattgct
     3121 gcatatccta gcatgtttac tggatctttg gctgagccaa atggcggtgc atacgtcaat
     3181 tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg
     3241 atccgtttat ccacaccttt tgccccaaca ccttgtgcac cataaatctc gccagtctct
     3301 ggatggaaga tcaacttcag taaaatatct gttgcttctg gataatagct tgcatgatct
     3361 tttcctgata catgaatgac ttcaaaagac agttcgtttt ctttggctgt tctttcactt
     3421 aaaccggtgg atgcggcagt cattccaaac gttcgtataa ttgctgttcc aataccgcct
     3481 tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg acgattggct
     3541 ggtgaagcaa gagaaataag tgcatctttt ccagtgattt gctgttttac aacgatagca
     3601 tcccccaccg cataaatatc aggaatattc gtttgataac gttcgtctac taggataccc
     3661 ccgcgtatgc ctaattcgat tccagcggtc actgctagct ggttatcagg acgaacacca
     3721 atggataaaa taactaaatc agcagggatg gtacgcccat catccagtcg caatcgtttc
     3781 ccgtggtctt caaatccagc gacagcatga gaagtaatca cttctactcc ttgatgagac
     3841 aattcttttt ctataaaagc tgccatttct tcatccagtg ttggaagaat atgtggtgct
     3901 tgttcgatca ccatgacttc taaacctctt ctttttaggt tttctgccat ttccaatccg
     3961 atgaatcctg caccaacgat cacggcacgc tttggctgtt tttctaatgc atgcatcact
     4021 ttatcgatat ctggtacatt tctaatagaa aaaacattgt cggcttctgt tatccctgga
     4081 atcgatggaa caactggttt tgcaccagga gataaaacca atgcatcgta ctgttcaatg
     4141 tgtttttgac cctcatgtac gacttctacc gttttgtttt cgggaaagat ggctgtcact
     4201 tcatgatttg gacgcacatc taaacaaaaa cgagcggata atgattctgg cgtttggaca
     4261 agaagatttt cccgttcact gatttctccc gaaagataat aaggcaagcc gcaatttgca
     4321 aaagaaacat acggtccttt ttcaaaaaca acgatttctg catcttccat taatcggcga
     4381 agccgtgtag cagcagacat tccacctgct acacctccga caataacgat tttcatctta
     4441 tttccctcct actgttcgat caaaccattg attcatgcca ccacgtacat tcactacatc
     4501 ataaccagat ttctttaatt ctttagctgc ttgtttactc ctcatccctg attgacaaat
     4561 cacatagacg gtcttttctt tgtctccatc atatctgttt atactttgta atggaacatt
     4621 tttagctcct tttatatgcc cgccccggta ttctgcaggt gttctaacat caagtaacgc
     4681 tggattttcc aaaatctttt cctttaattc tgggactgtt atcgatttta ttgattgaaa
     4741 tagcaaattc attttctgca cctcatttac atttttataa atacctcatg gggtatatta
     4801 aacaggagct ttattaatat gtcaaccaga atgactcttc atttttttca ctacttttta
     4861 tatttgcata aaataccgat aaggggtatt atctaattat aaaaatagag gagaatgtgt
     4921 tatggagatt gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg
     4981 tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag
     5041 cgctgttcgt tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa
     5101 ttgctttgag aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat
     5161 caagatgatt attaaaaagt aaaggaatga tttcaatgaa agtcgttttc catattgatg
     5221 aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa gcttctccag
     5281 aaacgacgat tgtcgtatct gtaaatggca tcgcgattac cggttatcta gactctgcca
     5341 atgcagaatt cttagatttg caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg
     5401 ctaatcatat ttcagaaagt tctttacctg aacaagtaat cgttgtacca gcaggtgtcc
     5461 ttgatttagt tgaactgcaa tcccaaggtt atgcttatat caagccttag tcagtagagg
     5521 ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga agctacttct
     5581 ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc
     5641 aaacaaaaat agcttctcat atttttcgtt tggttgggct tattaccgca gatgcaagct
     5701 tttgaacacc gtttaaggaa gaggttgtga aatcagcggt ttgacctaag tattagagaa
     5761 aaaaacggga aaatagctcc tcatattttg ccgttttttg agctaatacc gaaggtcagc
     5821 tgattgaaca ccgtttacca agaggttgtg agcctaccat ttaactccga atcacaagaa
     5881 ataatttcaa aaacaacttc tcctgttttt ggtttggttg gcttattatc gagaagtagc
     5941 tactgggagt ctatttatca aaaaaaggaa tgcgacatag gtcacacccc ttttttattt
     6001 tctttgaaac atctttcggt actcgacagc atacaagaca aaagattgta aaagaccgta
     6061 agtgatgacg gcaaaaatga ctctcaccca cagcgttggt aattctggga aaagcagcaa
     6121 aaggatcatg ccaacataga tgacagcagt cgctactttg ccataccact tcgctccact
     6181 taatttgata tttttagata gcccgattaa tccagctaca aacataaagc cttcttttat
     6241 caagaataga ccaaacaaca gccaaagcca cggtcgatac catgccatag acaaaatcag
     6301 aaaaagttgt gtcaatttat ctgcaaacgg atcaagcacc ttccctaagt ctgttacctg
     6361 attatatctc cgagcaatga ccccgtctag ataatcagac aaacctgaga caacaatgac
     6421 cgctcctgct atataaaatg actgtctcag cacaaaataa agataaatcg gcagcaaaaa
     6481 aatacggaat atcgataaaa gattcggaat cgtttgtatt tctttacgcc agtctgtgct
     6541 taatttcatt ttatcgccct acctcctcag cactcttttc tttttatttt agcttacacg
     6601 gggaataaaa ccaatgattt cactgttttt atttttcttt acagccactg atattatgag
     6661 ctaatttgag ataatcactt gctgttttca tcgtttactc ttctttcttc ctgtcgtttg
     6721 cgcatttctt ttactagtac tttatgagct ttttttgatc ctcgctctag gtcacgctgc
     6781 cgtttacgtg ttttataatc gataaacaga ctgtctaaat cgtaacctgt cgggtacaga
     6841 tcttctgctg aacgcaccag acgaacacgc tgacgaggca cgtggattat ttttttgtct
     6901 aaatatacat caatcgtatc ttcacctata tcttcataaa ctaacgcttc tttttggtat
     6961 tcattgacaa atatccgatc accttttgca aagagctggc gttctttttt attctccttc
     7021 ctatttgtaa ctgctgtctt aggaagaaat tcttttttct gtgtccgata ggctttttcc
     7081 gccaagtaac gctctgcttg atggatcaat ttcatcgaca tatccatttt atgagcaatc
     7141 cacaaagctt ggctttctcc tgtttctcca acccgcaact gatatttagg acgaagtgct
     7201 tcccgatcga aagccatagc agccggcaca aaatcttcat gatctccggc gaatttcttg
     7261 atttctccat aatgggtcgt ggcaatgatc aatgcccctt gctcatacat agattccatg
     7321 attgcgatcg ctagtgcagc cccttcattt ggctctgttc cactgccaat ctcatctaaa
     7381 agtaccaacg tattcctttt tatctttttc aaaatagcag cgatgttttg catatgacca
     7441 gaaaatgtac tcaacgcatt ttctaaattt tggtggtcac cgacatcgac gaaaatctca
     7501 tcaaagacag caagctctgt cccttctttt gccggaactt gcataccgaa catcgccatc
     7561 aaagtcaata aaccgactgt cttcaacacg actgttttgc ctccggcatt tgctccggtg
     7621 ataatcaagc ctcgatagtc tttaccgatt tcaacatcca atggtaccgc atgctcaggt
     7681 aaaaatggat gtctgccttg cttgataatg atatgttccg atttattgat tctaggtgtg
     7741 cttccattga tttctcggct aaattttgcc cgagcaaaga tgatatctaa caccgtgatc
     7801 gtttcgatca ataaatcgat cacttcttcg ttttctgcga tagcaccagt caatgccgcc
     7861 aggacttgat attcttcact gatttcttca gcttttaaca actgatagtg ttcattcaat
     7921 ttagaaatca cagttggttc aataaaaacc gtcgttcttt tatttgattc atcgataatc
     7981 gttccatcta ttttattttt atagctggct ttgataggaa tcgtatagta ttcgcctttt
     8041 tgaacaatca ttgcttcttg gatcatttct ttgtttttcg gatgacgcaa gaatttcagt
     8101 aattttgact ggatttcttt ttcgattagt tgtaattgtt tgcgtacttt acgcaaatta
     8161 cgtgacgcgt cgtcactgac tttattattt tttattttct ggtcaatcaa ctcttctaca
     8221 tttatcaaat caggaagatg tttgctataa gcaaacaaca agggtgtctg gtattgattc
     8281 ttatcaaaaa actttgtgat catccgactg cttctaagaa aatccgcata ttcgatcaaa
     8341 tcggttggct gcagaatcaa cccttttttg acttgttctg tcagtgcatc gatcctcggc
     8401 aaccccataa atggaacatg ctgactgctt tctaagatca agcgtgcttc tcttgtttct
     8461 tcttgacgat ccaagacagt ttgtaggttt gtcgagaccg tcatatcgct gattcgtttt
     8521 ttgctatagt ttccaattgc tcttgcttgg atttcctttt tgatccgatc aaattgtaat
     8581 tgactaatca tttcgttgtc catattctct ccttttcttg aagagatacc tgtcccaaaa
     8641 taaataaggg cctagagata aataaaagga tagaacacag cttaaaaaag tcatgtccta
     8701 tccttattag aatacaaaga tattgttttc ctgacaggta ccgtttcata agcacaccaa
     8761 ataaactttg tttattggct attcgactta ctgattagat accagctaca ctcacaaaac
     8821 ataccccatt cttctaggtc ttatttattt tccacttaac tatagcaaga cacattttgg
     8881 ttgtcaaact ttagtatgtt ttatagattt acaccattaa tgaatagaca cctaccgcga
     8941 taattgctcc gatcattgga cctgcgattg gtacccatga atatgcccaa tctgaagttc
     9001 ctttattagc tattggcaat accgcatgtg ctaatcgagg accaaagtcc cgagcagggt
     9061 tgattgcata tcctgttggc cctcctaatg aaagaccgat ggctaagatc aatccaccta
     9121 cagcgaatac gttcgttcca ggtgcaaatt cgttttgtcc aaaagctagc aacccaagaa
     9181 ctaatacaaa tgtaccaatt aattctgtga tcacatttgc tggataattc cgtatagcag
     9241 gacctgttgc aaacgttcct aaaatcgcgc tttcatcttt cgttgcattc caatgtggca
     9301 aataagaaag ccaaacaaga atggcccctg caaaagctcc taaaacttgc gcaatgatga
     9361 aaggaacgac aagattccaa ctaattgccc ctgtcatcgc catagctaga gagactgcag
     9421 gattcaaatg agctgggccc atgaatccag aaatatagac agccattgtt acagcgagtc
     9481 cccaaccaaa tgcgatgaca atccagccag aagcctgtgc tttacttttg tttaaattca
     9541 cagccgcaca cacaccatct ccaagcaaaa ctaggatcag tgtcccaata aactccccca
     9601 tgatttgagt catatcactg ttcatttatt tctccccctt tagttcccgc agatctgatt
     9661 ctgcaatcaa tgcttctaaa cgtttagtct gttgcgcctt ctcctcttct gtccattcaa
     9721 aatatccagc aattgcatcg atcacaggtt gcttgatctc atcaagctga tctcgttcaa
     9781 acaacagatg atttgtacga cgaatgagat aatcgcctgg ggccaaaacc atttcttctt
     9841 ctaaaccata acgtaaccga gctgactcag cgagactcaa accaggataa ggtgccattt
     9901 cttttgctaa ttcaaagata cgtcgggcat tagtcccgta aaaatcagcg atataagtag
     9961 catcttcttc ctctaaaccg gctgctactc cttcttttgc taattctgtc actgtttctt
    10021 ctaatttagt tggatcgaag tttccacctg aaatctgata ttttttagag tcgatttctt
    10081 tcgtctctat tccgtattct tcttttaata attggcgtat caatcgcaaa gctccttctg
    10141 ccatcttacg gtaatcggtg attttcccac cagataaagt cagcaaacca tctggttctc
    10201 tttctaaaga actacctctg gaaatcgtag aaggtgcttt ttcatcacgg ctgttttcca
    10261 aatggttcaa cacgtcttct acttcagcac gagaaacttt gttttcctta tattcactta
    10321 ctgtatcgac aactttattg aagctcttgt ctgaaatcga tccattatct ccaccattgt
    10381 aatcagaacc agaattacca atcaacagtg gacgaagtcc tgcccagctt gcttcgatat
    10441 cattcaatgt gatatttgct tctggatagc gatgattgat cacatccaat agataatcta
    10501 catcttcttg tgtgactttc ggatccgtaa aatctccttg gtaatccgta tctgtcgtac
    10561 caaagtaagt cttgttttct cttggaatag caaaaaccat ccgcccatct tgctttcctg
    10621 tatcgaagta tgtgggttgc ggtacaggca gttttttcgc atctacaact aaatggatcc
    10681 ctttagttgg acgcatttta ggagagactg gacgcgtaaa attcaagttt cgtactttat
    10741 ctacccaagg accactcgta ttgatcacta attttgcttt gatttcgatc acttcatctg
    10801 tcagcaggtc acgagctttg acgccaacga tttgatcgcc atcatataaa aagccaaccg
    10861 ctttcatttt acttactaga taagccccat cttcggcagc ctttttgata ttatcaatca
    10921 ctaaacgggc atcattgttg cggaaatcta gatagacacc cgcgcctttt agcccttctt
    10981 ttttcaaaaa cggttctctt tccaatactt cttctggtgt cagcgtataa ttctcatatt
    11041 tggttcctgt cacatttgcc agtttgtcat aaaggtccat tgctactttg accgagaaca
    11101 tattgaaggt tgttgctcct tcgccctcat agattggcaa aagcattgga tctggttttg
    11161 ggatgtgtgg ggcaatactt tgtacgactg cacgttcacc aactgtgtca gctactactt
    11221 ccacatcaaa tgtcttcaga taacgaatac cgccatgaac aagtttggtc gagcgagagg
    11281 aagtcccttc tgcaaaatcc tgcatttcga tcaatcctgt tttgattcct gatgctgctg
    11341 actggactgc cacaccggca ccagtgattc cgccaccgat gatcaacaga tccaattctt
    11401 cttgctgcat tttttgaatg ctatcttgtc ttgtcttgtt tgaaaacata tggatttcct
    11461 ccttattcaa atgcttgggt agcttttact gcttttttcc aaccgttgta aagtttttct
    11521 cggcgctctt cctccatttg aacttcaaat agcttgccag cttcataaaa ttcgcggatc
    11581 tcgtctgtat ctttccaata accgacagct aatcctgcta aaaatgctgc ccctagcgcc
    11641 gttgtttcca agttttctgc tcgttggatc ggtacattca agatatctgt ttggaattgc
    11701 atcagatatt cattgtttgc tgcaccgcca tctactttca atacaggaat cttgattcca
    11761 gtgtcatctt gcatcgtatc gatgatatct ctcacttgat aagcaatcga ctgtaaagta
    11821 gctttaatga tatcttcttt tgtcgtacca cgtgtcaagc caaacatagc ccctcgtgct
    11881 gcctgatccc aataaggagc acctagccca acgaatgcag gaacaacata aacctcatct
    11941 tcgcttgtag agcgtttcgc tgcgtcttct gaatcgctag ctttttgcaa catttgaagt
    12001 ccatctctca gccattgaat cgaagaacca gccacaaaaa tacttccttc taaagcatag
    12061 taaacctttc catttatgcc gtaaccaatt gtcgtcaata aattattttt ggataattgc
    12121 ggtttttcac cggtattcat gacaataaat gaacctgtac cataagtatt cttgaccatg
    12181 cctggttcga atgccatttg tccgaataat gcggcttgct ggtctcctgc catccctgaa
    12241 attggtgctt cgcttccata aaaatgataa ccttgcgtat aaccataaac ttctgagtta
    12301 gaagtgactt ttggtaacat cactcgagga atatttagta aatctaaaat ctcctgatcc
    12361 caatctagct gatggatatt gaacaacatc gtccgactgg cattcgagta atcagtaaca
    12421 tgtgcttgtc catcggtcag tttccatact aaccaagaat cgatcgttcc aaacatcaat
    12481 tcgccttttt cagctcgttc ttgcgcgcct tctacatgat caagaatcca gcgtatcttc
    12541 gttgctgaga aataggcatc gatcactaac cctgtttttt cgtggaaaaa ttcttgatag
    12601 ccttcatttt ttaagttatc tgcaattcca gctgactgtc tggattgcca aacaactgca
    12661 tgatagattg gacgtcctgt atctttttcc catattactg ttgtttcacg ctggttcgtt
    12721 atgccaatcc cagcaatctg atttggtttg atccctgatt cgatgaacgc tccggcaata
    12781 actgactgta cagaattcca gatttcattt gcatcatgtt ccacccaact ttcttgcggg
    12841 aaatattgcg tgaactcttt ttgagaactg ccgatgtgtc ttcctttctt gtcaaagata
    12901 attgctcgtg agctagttgt accttggtca atcgccatga tatacgttga ttctgtcatt
    12961 gtgattccct ccattatatg caaacgcttt ctttaactac tcttatcata ctactgtttt
    13021 aaaaaaagaa actgtcaaaa ttttattctt tttttctttt tttgacagtt ttttattatg
    13081 aagctatttt ttaatcaatt cttttctttt ttcagcgtct ctattcttct tttcaatcga
    13141 ataatgtcgt aaggtgacgc aaattctgac agataatatt cacactgttt tttgtttctc
    13201 tggcaaaatg tgatcaccaa atcatattct ttaccttctt cataacttcc aatctcaata
    13261 ccgccgattc ccctcagttc tctgatcaga tattgttgaa aagcaatacg ataaatcggc
    13321 agactttcta aatcgattcc aatcactact ggttttgcaa tataaaaatc gatcatcaaa
    13381 agaacattgg catactggat catcaggtaa ctaagctcac tatcttcagc tcttttcctc
    13441 ggcaattgct gcaccgctgt ttcctgtaat ttcgccagta actgtgttaa ggaatgcccg
    13501 agcattttct gctgctgttc caaaagccga tcacgctcat agacttcgat ctttccaaca
    13561 aagaaaaacc attcattgtt gatctgggaa atttggtagc ctactgcctt ttcttctttg
    13621 attttcaaac gacgaggacg ataatgccat aacatcgttt cccgaatgta agtatctaaa
    13681 acagcggttg gtaacttttt gctccgtgtc aaatcatact gataaaatat ttcttcgtct
    13741 actatcgaaa aagaaacaaa aaagctgtaa aacatcattg tttcgtatac attcagttct
    13801 gctgctgttc gactaagatg catcacgatg attggatcga ttgcttgata aagccggtcc
    13861 gactgataga gtaatttctt ttctttcaaa gttgcgtacg tacttttttc attcaatagt
    13921 ctttttttcg taatcccgag ccagcaagca atctgtgctt ctgctgatgc cgtaaacgtt
    13981 gtttctaata cacggtccaa tcctagaatg aacggtcttt tttctggtgt attttgcaaa
    14041 aaaagcggtc gctgatcttc cgggatatat tggaatagtt cataataaaa atatcgtatc
    14101 tgtagctctt ctccgtataa ttgaccgttt ttgatttgta atccaaattc ctctaagagc
    14161 tggttcaatt ctttgatctt tcgaaaaaca gaagactcac tgatattaaa cgctgtcgct
    14221 aatcgaacga tggatacttg ctgatgttct aataaataga cgagcatctt aaacttcaag
    14281 ctttcctgca ataaaaacaa tagaattttt tccaaactta catcttcgtc caacacaagc
    14341 agcgcctgat actcatttcg ctgtacttct actttttttt ccatcattcg gccaatcaat
    14401 tcaatatctt ccaaatattg atcaaacgag gcttttgaca gttggacatg ctctctcaaa
    14461 ccattgatag aggctgttcc cccttgcaga acaaggtttc gcaaaatcag gatctgtaac
    14521 tgctcttttc tttctaataa tgcttcaatc ttcataggca gtattcgcca cctttgacca
    14581 caccatatct ggcacacggt attctggctg gaaaaatagc tcatgttctt ctaactgttc
    14641 aaacgtccat ccttctttta ctgcaattgt cagttggtca agaaggtgga acatctcttt
    14701 tttgctttgg agctgtgctc caagaatctt tttttcagaa tcaaatagaa atttgagttt
    14761 aactttgctt tgatttattg ctgtaagctt gtagtctacg tatcctgttt caattttttg
    14821 gggataaaaa atgctctctt cttctgtcaa acctacgctt cctaagaaat agccaaacca
    14881 ctgattgccg actggacgat aggttccctg atcctttgtc tgtgagccac taattgtctt
    14941 gctgacattt cgtgctgtac ggacagcgtt atttactaga gaaacataca ttttttcatt
    15001 tgttggacga aaagttactt ggatcgcatc gccaatcgca taaacgtctt tttgagacgt
    15061 ttgcaagtag tcatctaccc agatcgttcc atcatcattc aatgttaatt gttcttccca
    15121 catctgattg tcggggcgag aatgattagc cagcaaaaca agatcatagt tctcctttct
    15181 tttttccgtc tctacaatag cggtttcatt ttcggtcaaa gacacaactt gttcttctaa
    15241 aaaaagtgaa agcccttgat ttttcatttc ctttactaac ggttctgtca tttcaggatc
    15301 tagataacga aaaagaattg actttcggct ttcgtaaaga tggatttgtt tttttcttga
    15361 tgccaaccct tcagctattt cgattcctgc ttgtcctgca ccaataattg caattttctt
    15421 tgcattcatg atcttttgct gaagagccgt tacgttttgt atctttttaa ctgggtgaat
    15481 aagcgttgat tctgcagaaa tatttcgaaa cttttgattt gaaccagtcg ctagaatcaa
    15541 acgatcaaaa tccaattttt ttcctcctgc taaaacgact tttttttctt tcaatcctat
    15601 tactgtcttt tctgtgtaaa catggattcg atagcggtca gtcagttctt ctttcgtgat
    15661 ccaagaaagt tgtttttcac tggtaacctt tccttttaat atcaaattga acccgctggg
    15721 aatccatcct aacttcgatt gcttttcaat caaatatatt tcacttgttg gcatcaattg
    15781 gcgaagataa atagccgcat gaatccctgc aaacgacccg ccgacaatta caatgttcat
    15841 ctgcattcct ctttcttata aggtgttcta ctgctaactt ctcaatcaga aaacacagta
    15901 ttcataactc gctttcaacc ttatgataca tagcgatgca gcagaatcaa ccctctccat
    15961 cataccgtgt tctaggtctg cttctcaata atgtactagt ggacctaatt gaagagtatc
    16021 atttctgaag agatgtttca aaaaatgtac tcttataaaa gaaaaaaact cgccttttac
    16081 gtacctctgt ccaaatagga aacagaaaag aacgaaaaga cgagttttag cgtgtgtaat
    16141 gtaatagtta gaatagacac atcatttatt tttaattctt taatgcttca acaactgctt
    16201 cgcctaattg atgagcagat tgaggatttt gccctgtaag aagccgccca tcaatttgaa
    16261 ccacttctga aaaagctgct ccagactgat agtttgctcc tttttctttc aaagcatctt
    16321 ctgctaagaa tggtacttca ttcgttgtac catttgcttc ctcttcttca ttactgaacc
    16381 cagtcactgt acgtccagct aaaatagatt ttccagactc atctgtaact ggcaacaatc
    16441 ctacaacacc gtgacaaaca gctgagacca gcccttcatt tcgatagatt tgtgaagcga
    16501 ctttcccaag atctattgct tctggaaaat cccacattgt tccgtggcca ccagcataat
    16561 aaaccgctgc ataatcattt gcatccacat catcaggacg taaggtattt cccaaggccc
    16621 gatttctgaa atcttcatct tcataatagg cccaatcgat ttcatccata ttcaaaacac
    16681 tttgcggttc taaaggtaca tatccgcctt taggactgac aaaatctgca tcgatcccat
    16741 tttttactaa tacatcataa aaatgagtca gttcacttaa ccacaaacct gtaggacgct
    16801 cgatcttttc gtacttttct acatttgtta atacgatcaa tactttcatt ttcccatcct
    16861 ctttctataa aaattgcgtt attgataacg tgctttcaag aaattatata atgggcttgg
    16921 caaataaagc tctttatatg cttcttcaaa aggtttcgtt acaaattcat cctcacggtt
    16981 ttccaataat ttctctgccc agtcaggatc gacgatcatt tgctgtccaa ttgctgcaag
    17041 ttctgcatat ttcaatacct gctctacatc ttcgcgattg cggatcccac cgacaccaac
    17101 taaagggatc tttccttgaa tcgtgttatg gaccacttca agaatcgatt tatcgttgta
    17161 tttatcagaa cgagctattc gatcatacat atttaatgaa acatgcagat aatctagttt
    17221 tgactcacgt aatcgttcaa gaagccagat cgtgtcttca aaacggatac ctggtgtttc
    17281 atattcttct ggtgagaaac gataccctac gatgaatggt tttttagcat attgttcaac
    17341 cgctgagaaa accgttgtta ataattcttc aataaaacga taacgatttt ccaagctacc
    17401 gccccattga tcctctcgtc tattcgaatg cggagagaaa aattgttgga tcaaatacgt
    17461 atttgctcca tgcaactcga ttccatcaaa tcctgattca atcgctctac gtgtggcttt
    17521 gccaaatgct tggattgttt caacgatttc ttcttcactc attgctcgtg gtgtttccgc
    17581 atcaggacgt tttgctgcaa ctgcacttgc agatacaggc tgttctccag ataaagttgc
    17641 tcgacttgtc attcgtccac cgtggaaaat ctgaataatc gcttttgcgc cttctccttg
    17701 gattgctttc gctagctcac ttaatcgagg aatcatttca tcatgagcga tgctgagttc
    17761 acctggccag ccttttccga tatcttggac atttgcagca ccagtaataa cagcaccaag
    17821 acctttagaa cgttgtttat aataagtgat ctcatcttcg gtgatcactc cattgaaaaa
    17881 gctgagttgt gtcgtcatag gcgacagcat caatcggttc ttcaattgaa ctcctgaagg
    17941 taaagtaatt gattgatcaa attgtgacat tgtcattcct cccataattc gctctgatta
    18001 acctatcgag aatcatctat cattaaacca ctcgattagt tgtacatgca actaatttgc
    18061 ttaaaaaatt ttattttttt tgtaacttat ctgttgcatc attcattgtc tgggctaatt
    18121 ctttacttgc ttcatttccc aaaactgctt tgtatgactg atgaagttct tcccagctac
    18181 catcgacttc cttagcaaat tggcgtcctt gttctgtaag ataaactaat gacaagcgac
    18241 cttcgctaac tgttttgacg tatcctttat attctaattt ttcaacaaaa cgagcaatcg
    18301 ttgatggggc ggaatagagc atttgtgcaa tatctttttg tggtattcca tctttttctt
    18361 ttaatatcaa taaaataaaa ccataagttg gtgccagacc tgtccttcta aacgcttcat
    18421 cagctaattt attcaacatg cgatctagct ttttcacgct aaagaaaaga cattcatcta
    18481 acgctttttc aattggcttt tccatttcat cccctccatt ggcgtacata tcatagtatg
    18541 atttttattt tttgtcaaaa aacacggaat aatgtccgcc tcctctgtct agatttgaaa
    18601 aaaagaagta gccatcttca aagaatttcg gcttgttttc gacaaaactc cttctggaac
    18661 accgtgaatc agaggttgtg aaaaagtcgt ttagttttga gtatgaagaa aaattttgtg
    18721 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgaaaaact ggcttttgaa
    18781 caccgtttat atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa
    18841 acaaaaatag ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg
    18901 tacaccgttt attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt
    18961 tcaaaaaaca gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg
    19021 agaatactgt ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt
    19081 tccttttatt ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat
    19141 tttttcttta accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat
    19201 ccataacgtt ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac
    19261 gtataaccaa ataaatctac tccatcttcg atagcttcct tcatctgctc aatatgctgc
    19321 ttcaaatagt caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata
    19381 gctccgagtc cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc
    19441 aaagcaattc tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac
    19501 ggatttttaa ttcctccagt tattaagttt ccttggaccc cttccaaatt ttctggatga
    19561 gcactgactg cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct
    19621 aagatttctt catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt
    19681 tgcgtatacg ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt
    19741 tgctgctcaa ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga
    19801 tagctgatca tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct
    19861 aaagcacttg ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga
    19921 tggttttctt caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca
    19981 ttgaaagtca tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa
    20041 cgaacgtaaa actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat
    20101 aacggcattt cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg
    20161 tcaaacactt gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga
    20221 ggaaaaattc ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat
    20281 aaacggatgt cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa
    20341 tacttgctgt tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga
    20401 tctgtccatt tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat
    20461 cctttttcat ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat
    20521 aaaaatcctt ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt
    20581 gattattttt cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa
    20641 ccattgtggt cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata
    20701 aatcgccttt acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta
    20761 aattcactgt atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc
    20821 caattgcatg tttcgttgga aaaactgttg aaacttcccc gtttacagga ctgtagattt
    20881 ttccgtccgt tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat
    20941 cgtttacttc agataaatca attatttcac ctgctgcagg agtacttaac actatatcct
    21001 ttgaggattc ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac
    21061 tgatcatcgc taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg
    21121 ggaaagacaa aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta
    21181 ctccaccaaa tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag
    21241 tcacaccata aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag
    21301 ataaactttt tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt
    21361 gcgcagctac ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat
    21421 tgttggtcat tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa
    21481 atccgccgat aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag
    21541 ctgcaatcag ctgacttgca taatctgcaa ttggacctac aactaataaa gtaattggga
    21601 ccatcacgat gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt
    21661 tgcgaatctt aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg
    21721 tacttgtata tcttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac
    21781 tatatgcact gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac
    21841 gatctgtatt gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa
    21901 atgcatcagc tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca
    21961 aactcgtaca taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag
    22021 tcggggcaaa aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag
    22081 ttcctctctt tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat
    22141 acacttcatt tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag
    22201 tgatcacacc ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg
    22261 taaagcgtaa ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa
    22321 atcggacaat atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta
    22381 atgccgtagc aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc
    22441 aattagtacc ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc
    22501 cgagcaaatc actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg
    22561 agacaaaaaa aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat
    22621 actctgtgat ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat
    22681 ttaacggaag cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct
    22741 ttttatggat ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct
    22801 ttttttcttc aactatggtc agcttttcac gcaaaatctg ataaatctcc gaagattcaa
    22861 actcatctag aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct
    22921 cagtagtcaa aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca
    22981 ctggttctat ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta
    23041 gtgtattcaa aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa
    23101 aaggattcaa cgcttcggca agatacaaaa ccaaataagc gctttcagct aaattgggac
    23161 gtaattgaaa atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg
    23221 agatgatctt ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca
    23281 tttgtttgat atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat
    23341 ctaaaaaacg aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt
    23401 tttcatcatt aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt
    23461 cttgattgga aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca
    23521 tcggaataaa taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg
    23581 tcaaaacgag ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg
    23641 atataagaac ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac
    23701 tgatttgttg aggcaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga
    23761 aaattctttt tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca
    23821 gttctaattt aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg
    23881 ctgtttttga aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa
    23941 acaataagta cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt
    24001 tcgtcacctc ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac
    24061 ctgaacgttt atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga
    24121 tttcagtttg gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta
    24181 tccaatgatt cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca
    24241 tttgcttaca cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt
    24301 cacttgcaga ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta
    24361 gactggataa tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg
    24421 ctcccatcat tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt
    24481 cctgccgata cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct
    24541 tttattgcct cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg
    24601 tggattcgat tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat
    24661 aatggtaatt cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca
    24721 attggccaac tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt
    24781 gctccgccag tatctacgcg catttctgct tcttttttat agacagcgct acgataataa
    24841 ctaaatgcaa tataatctac cgtgttagct tgcaagtact gttcgtctcc atctaacatt
    24901 actggtgcac agtcttcttc cttccatatc ctgtaaatat aagctggata atgccctcgg
    24961 cacatgacat cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc
    25021 tctggatcac agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc
    25081 ttagcatttg gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata
    25141 tgatgacagg cttgataaat ctcttttttt gttagtgtag caatgggttc aagtttatct
    25201 ctagcaggga atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc
    25261 tcattaaagg tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca
    25321 taaaattcaa aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat
    25381 gctaaggggg tttcataatg actaagagta attactggct cgatatcata tcgatttaat
    25441 gtattgatta gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct
    25501 cttggaaata ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc
    25561 agtaaacgaa tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa
    25621 tggagtgatt cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta
    25681 ggatgctggt tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc
    25741 aaccatgcac cttcacattg attagcagct actgctccgc cccataaaaa attttctgga
    25801 aatatttcct gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata
    25861 gctcgattca tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga
    25921 atgctactct tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga
    25981 aaacactcgt taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata
    26041 aggtcgtaaa tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac
    26101 ccttaaagct tcacgtggaa cagtgaacgt ctgcttttca ctggacatat tacaaataac
    26161 gagccattgc tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc
    26221 cactaattca tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt
    26281 cttgtacata taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt
    26341 ataatttgga ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc
    26401 gttccattgc ataggcgtcc tagcattgtc tcttcctttc gcattgattg attccaagat
    26461 ttcctctttt gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc
    26521 ttttgcttcc gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata
    26581 gatataaggg gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt
    26641 tcgatacaca tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca
    26701 aaataaacta ttccatcctt catcacctaa agatgtttgc catttggata atactttttt
    26761 cagttcagag atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa
    26821 tcctatgtgt tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag
    26881 tttagcgatt tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc
    26941 aaaagttgct tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat
    27001 ttctttatcc ggttctttgc caatcatatc gatcacgtcc atacgaaagc caccaatacc
    27061 tttatcgatc cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca
    27121 attcaaatca ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc
    27181 caactgccaa gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac
    27241 cggatcacgc caaatataat agtcccgata aggattttct ttatttttac gggcctcaat
    27301 gaaccacgga tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt
    27361 tcttttcttt gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat
    27421 gattgcttga taattactga tatcataccc attgtcatca ttcggagact cgtatactgg
    27481 agaaagccaa attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc
    27541 ccgcaaatct ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata
    27601 aacaactgaa tggtgccacc atttttgttc catg
//
LOCUS       DACEZN010000019        26990 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEZN010000019 DACEZN010000000
VERSION     DACEZN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.019"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            214..1500
                     /gene="efmA"
                     /locus_tag="IXH60_000637"
     CDS             214..1500
                     /gene="efmA"
                     /locus_tag="IXH60_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1989602.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1638..2237)
                     /locus_tag="IXH60_000638"
     CDS             complement(1638..2237)
                     /locus_tag="IXH60_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1989603.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2641..3762
                     /gene="mnmA"
                     /locus_tag="IXH60_000639"
     CDS             2641..3762
                     /gene="mnmA"
                     /locus_tag="IXH60_000639"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1989604.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            4008..5429
                     /gene="arcD"
                     /locus_tag="IXH60_000640"
     CDS             4008..5429
                     /gene="arcD"
                     /locus_tag="IXH60_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1989605.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5443..6561
                     /locus_tag="IXH60_000641"
     CDS             5443..6561
                     /locus_tag="IXH60_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1989606.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6621..7913)
                     /locus_tag="IXH60_000642"
     CDS             complement(6621..7913)
                     /locus_tag="IXH60_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1989607.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            8063..8623
                     /locus_tag="IXH60_000643"
     CDS             8063..8623
                     /locus_tag="IXH60_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1989608.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8752..9129
                     /locus_tag="IXH60_000644"
     CDS             8752..9129
                     /locus_tag="IXH60_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1989609.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            9062..11134
                     /locus_tag="IXH60_000645"
     CDS             9062..11134
                     /locus_tag="IXH60_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1989610.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11345..12292)
                     /locus_tag="IXH60_000646"
     CDS             complement(11345..12292)
                     /locus_tag="IXH60_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1989611.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12538..13002
                     /locus_tag="IXH60_000647"
     CDS             12538..13002
                     /locus_tag="IXH60_000647"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1989612.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(13064..14107)
                     /locus_tag="IXH60_000648"
     CDS             complement(13064..14107)
                     /locus_tag="IXH60_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1989613.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(14484..15449)
                     /locus_tag="IXH60_000649"
     CDS             complement(14484..15449)
                     /locus_tag="IXH60_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1989614.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            15683..16528
                     /locus_tag="IXH60_000650"
     CDS             15683..16528
                     /locus_tag="IXH60_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1989615.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            16719..17906
                     /locus_tag="IXH60_000651"
     CDS             16719..17906
                     /locus_tag="IXH60_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1989616.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            17912..18268
                     /locus_tag="IXH60_000652"
     CDS             17912..18268
                     /locus_tag="IXH60_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1989617.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            18270..18608
                     /locus_tag="IXH60_000653"
     CDS             18270..18608
                     /locus_tag="IXH60_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1989618.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            19003..20037
                     /locus_tag="IXH60_000654"
     CDS             19003..20037
                     /locus_tag="IXH60_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1989619.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            20030..20716
                     /locus_tag="IXH60_000655"
     CDS             20030..20716
                     /locus_tag="IXH60_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989620.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            20740..21588
                     /locus_tag="IXH60_000656"
     CDS             20740..21588
                     /locus_tag="IXH60_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1989621.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            21785..22558
                     /gene="sufC"
                     /locus_tag="IXH60_000657"
     CDS             21785..22558
                     /gene="sufC"
                     /locus_tag="IXH60_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1989622.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            22571..23857
                     /gene="sufD"
                     /locus_tag="IXH60_000658"
     CDS             22571..23857
                     /gene="sufD"
                     /locus_tag="IXH60_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1989623.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            23854..25089
                     /locus_tag="IXH60_000659"
     CDS             23854..25089
                     /locus_tag="IXH60_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1989624.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            25076..25546
                     /locus_tag="IXH60_000660"
     CDS             25076..25546
                     /locus_tag="IXH60_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1989625.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            25551..26942
                     /gene="sufB"
                     /locus_tag="IXH60_000661"
     CDS             25551..26942
                     /gene="sufB"
                     /locus_tag="IXH60_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1989626.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         8827 a         4986 c         5814 g         7363 t
ORIGIN      
        1 ttgtaaggac gacgcaaatt agttgtcttt tttattttta aatttaacac atgttgttat
       61 ttttaatatt ttgctgattt attgtttcta agtaaagaaa tttaaattga aacgattgtt
      121 atttagggaa aaaggtattt tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg
      181 ctatacaaaa tgaaaaagaa aagagggatg tttatggaaa atgaacagtc ggtcgtttta
      241 acgaattgga agagaaatta tttgttcttt ttatcgggac agtttttgtc aggaatcact
      301 agtatggtcg tccaatatgc gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca
      361 attttgagtt ttgctacttt actaggaatg attcccatgg ttttactaag cccatttgta
      421 ggaccgctag tggatcgctg ggataaaaaa gctcttttga ttgtcacgga tattattgta
      481 gcgattttcg cgcttatttt agcagtagtc ggaacgattt cagaatcctt cccgatttgg
      541 ctagtttttg tgtctttgtt tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc
      601 cagtcgatta tgccgacaat cgttccatct tctcacataa caaggacgaa tggacagtta
      661 ggaatggtcc aatcagcgaa tttcatcatt gcgccagctc taggtgcggc tttgttttca
      721 gtcgttcctg tcaattacct tattttatta gatgtattgg gggcagtttt tggtgtcggc
      781 ttattgattt ttgtgaagat cccaaaagtt tctcctgaaa tattggaagt tccgcttact
      841 atttttaaag atgcaaaatt tgggctgcag caattgatgg ataataaagg gttatggtac
      901 ataacgatca atggtgcatt tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca
      961 ttgatgacgc tagattactt tggtggttcc gttggacagg cgggagcagt ggaagtagtc
     1021 tacgcagtcg gaatgctctt aggcggtgca ctgatcagtt ttataggaac atggaaagac
     1081 cgaatgaaac ctattattat agcctacatc attatgggcc tgacgatcgg tgcaagcgga
     1141 ttggttccaa acgatagtca agggttcttg tactttctta tcttgaatgc aggagcaggt
     1201 tgtgcaacgc catatttcaa tacactgctg atggcaatga tccagcagag ttatgaatcg
     1261 aatgttttag gtcgtgtctt agggaacttc aactctttga tgaacctggc aggtccaatt
     1321 ggattattat ttgcaggacc attagctgat cgtttagggg tagaaaaaat gttcttgttt
     1381 tccggaatcg ggattttatt atgcggaatc gttttatttt taacgtcagc tgcccgaaat
     1441 tatgataaag aattacagaa aaaactggta aaagaacacc atgagcaaaa agacgaatag
     1501 tatggaatgg agttgtgaca gaagtcttgt tacaactcca tttctgatcc taatgttcct
     1561 gtggcaataa gcctactcaa atgaaaaata tgagtaatta tctttatttg tttaatactt
     1621 gatatagggc agttttttca cgacctatcg tacttcctct ttgcttcata gatttctttg
     1681 atttgttgct cagataacgg agtgccaaat ggtagttttt cctttaagag ttctctttcc
     1741 ggtgcgtcta cgcctacatt gacatcgttc tcgataggta cgctgtaatg atggatatgt
     1801 ccatgaagat tgcggatagt gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa
     1861 tattgttgat ggtcgaattt aacgatggca cctacatcat aaaaataaaa tttgggactt
     1921 ccgtcagtaa gcctaggatc atttttttct aaataacgga aaaaagcgcg actgtcatgg
     1981 ttccctttga tcaagtggat tgttccttgc agttgagtga ccagttccaa aatttctgca
     2041 tttgcttttt catattgcgg atgcatagca aggtcgccta gatgataaac atggtcggtt
     2101 tcttttatta ctgcattcca gctagtgatc aactgttggt tcatttcatc cacatttgca
     2161 aagagccgcg gagcaaagtc gttttttcct aataagcgtt catggaaaaa gtgcatatca
     2221 ctgataaagt aattcatgta tcttttgcct cccttttttc ttagagtaaa caaaatcact
     2281 tatctgagca actcataaca tctaaaaaga aaaggttgta aatgagacgt gcagctttaa
     2341 gcgataagaa gcaattttga aaaatagttt ttcacaattt ataaaattat tccttattgc
     2401 cgaaaggctg gcccttttta tgagtatgcc aattttttat atcgaatgct caaaagatga
     2461 gtgatttttt ggttgactat catgtttgtg tactgaatag gtccagtaga acagtatttt
     2521 tacgacttga taattaaggg atgaaatttt cagaaatatg acgtataatg taaaatactg
     2581 aaaagttgcg accttcaact cgtagatgtt tcagaatcta attgaaatga tggtgatatc
     2641 atgacagaca acagcaaaac acgcgttgtc gtagggatga gcggcggtgt cgactcatct
     2701 gtaacagcac ttttgttaaa ggaacaagga tacgacgtga tcggaatatt catgaaaaat
     2761 tgggatgaca cggacgaaaa cggtgtatgc acggccacgg aagattataa ggatgtagcg
     2821 aaagtagctg ctcaaatcgg gattccttat tactctgtga attttgaaaa agaatattgg
     2881 gatcgtgtat ttgaatattt cctagccgaa taccgcgcag gacgtacacc taacccagac
     2941 gtgatgtgca acaaagaaat caaattcaaa gcattcttgg attatgcaat ggatcttggc
     3001 gccgattatg tagctactgg acattatgcg caagtcactc gcgatgaaaa cggcgtggtc
     3061 cacatgcttc gtggagtaga caataataaa gatcaaactt acttccttag ccaattatca
     3121 caagaacagc tttcaaaaac gatgttccct ctaggcggca tggaaaaatc agaagtacgg
     3181 gcaattgctg aacgcgcagg ccttgcgact gctaagaaaa aagattcaac gggaatctgc
     3241 tttattggtg aaaagaactt caaacaattt ttgagcaact atctgccagc aaaaaaagga
     3301 aatatggtca ctcttgatgg tgaagtcaaa ggacaacatg ctggcttgat gtattatacg
     3361 attggtcaac gtcaaggact agggatcggt ggcggaggag attcgcaaga accctggttt
     3421 gttgtaggaa aagatctagc gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct
     3481 gcattgtatg ccacaagctt ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg
     3541 ccaaaagaat tcaagtgtac agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca
     3601 gttcgcttat tggatgataa tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc
     3661 actcctggac aagcagttgt attttatgat ggaatggaat gtctgggtgg tggtttgatc
     3721 gaccacgcat atcaagaaac aaaagtttta caatacgttt aagcgatatt aaatgaatca
     3781 aaacagctat ggctaacaaa agaaacacct tttcttttgg atagcatagc tgtttttttg
     3841 caataaaatt aatttttatt cattttatac ttgaaatact tgtgtaaaaa gttcataata
     3901 tccaataata tgaataaagt tgctttttgt taagcaagag cagttgcaaa atagaaggga
     3961 atcttttgca agtgatttgt tcaattttat aagtgagggt gatggaaatg gaaaagaaat
     4021 cgacgggtat tagcaaatgg ggactggttg ctctggttgt cagttcgtca atcggaagcg
     4081 gtgtgtttgg gatcacaagt gatctggcta gttctgcagc tccagggcca gcgatccttt
     4141 catggataat cgttgggatt ggtattttag ctttggtatt atcgctgaat catttaggag
     4201 aaaagcgtcc agatctagat ggtggtattt tcggttacgc ggaagcttca tttggtaaat
     4261 taggtggctt catcagtggt tggggttact ggttgtctgc gtggctggga aatgttgctt
     4321 ttgcaacgat gctgatgagc gccatgggag aatttttccc gatattcaaa ggtggacaaa
     4381 atgttccttc tattttattt gcaagtattt ttatttgggg tctgacttta ttagtaaaca
     4441 atggagttga aggagcgagc gtcatcaata caatcgtgac gatctgtaaa ttagtgccat
     4501 tattcctatt tttaatattt gtatttatcg catttaaagt gaatatattc actgctgatt
     4561 tttggggtaa cgtatctgat aatctagtga atggaacagg gcagcaagga acgatctggt
     4621 tgcagattaa aggttgtttg atggttatga tgtgggtctt tgtagggatc gaaggcgcaa
     4681 gtgttttagc caaccgcgcg aagaaacgtt cagaagccca gcaagcatcg atcattggtc
     4741 tgctttgttt attgtttatc tatatccttg cttcgatctt gccatacggc gtcttatctc
     4801 aagaagagtt agcaactatt tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg
     4861 gaaattgggg agcagcgctg atcaatatcg gcttgattat ttcgatcatt ggttcttggt
     4921 tgtcttggac gatgctccca gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc
     4981 cgaaaggttg gggaaaaacg aataaaaaaa atgccccaac ttattcgtta gttgttactg
     5041 caatcttgac taatctattc ttattgactt tcttagttac tgattatgcc tatcaatttg
     5101 cctattcttt atgtacagcg gcaattttga tttgttatct acttgtcggg atctatcaag
     5161 tccagttttc tttaaaagaa ggaatcaaaa aacaagtagt gatcggtggg attgctgtag
     5221 tttttgaatt gatgggcatc attttagcag gattttctta tgtcctttta tgtagtattg
     5281 cctatcttcc tggtttttgc ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta
     5341 cgggaaaaga aaaagtaatg attgggctga tttgtgtggc cgcactgctt tcaatcgggt
     5401 tgcttgctgt aggctggatc aagatataag ggggaaagaa acatgaaaat cgcaaatttc
     5461 ggtgtagaag aatggctaaa taaatgggaa aagaaagcgg tatatgatat tgcacaaagc
     5521 tcgatcgaag cattgacatt agacgaactc gttggcttag atgggactag cgtaagtaaa
     5581 tttttcgaaa aacgcaaaaa cgaaccattg gactatggct ggatcgaagg atcagatgtt
     5641 ttcaaacaag aagtcgcttc tctttatcaa accgtcgatc cggaaaacat cttacaaacg
     5701 aatggagcga ctggtgctaa tcttttagct ctatacgcgt tagtggaagc aggagaccat
     5761 gttgtttcga tgcttcctac ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc
     5821 gctgttgact ttgttcattt gaaggaagaa gaagattggc agtttgattt agaacagcta
     5881 gaagccttag tcaaacctga aacaaaaatc atctgtttga atagtgcaaa caatcctaca
     5941 ggcacattgc tagatcggaa aactttagaa aaaatcgcag aaattgctcg tacagtagat
     6001 gcatatgtat tgatagatga agtatatgcg cctttaacgg ataaaggtga attcttgtcg
     6061 atcgtagatg tctatgaaaa aggtatagca accaattccc tatccaaaac ctactccatt
     6121 ccaggtattc gtattggctg gacagctact ggacctgagt tggcagaagt ttttcgaaag
     6181 tatcgagact acacgatgat ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca
     6241 ttgaagaata aagaaaaaat attggcgcga aacaaaaaga tcattacaga gaaccttgtg
     6301 attttaaaac aatgggtagc caatgaaccg aaagttgaac ttgtcgctcc caattacgtc
     6361 tctacttctt tcattaaatt agccatcgaa gaagatgacc agacattttg tatcaattta
     6421 ttagaagaaa caggagtact attagtacca ggaagtgcct ttgatttacc gaagcatgcc
     6481 cgactaggtt actgttgtaa aaaagaaacc ttggaaaagg gattattcct cttatctgag
     6541 ttcttgaaaa aacaggccta aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa
     6601 gcagtgtttt tcatttacgc ttattgtcta tgagaaggag agtgccctga agctatggag
     6661 actcctgaag ctttttcttc tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt
     6721 acaagcaaga agttgaccaa aacaagaaga gaagtcgaaa gaaagacgcc gcgataacca
     6781 aatgcactgg aaacactgga gccgatcata ggccctacta cgttaccagt tgcttggaaa
     6841 gattggttat aactgaagat ccgtccagca gcttgatgag gcgaatattt tgtgatcaat
     6901 gcttggacag cagggagcag acaagcatca gagataccaa tcaaaaaacg taaggcagct
     6961 aattgccaaa cattcgtgac gaatgccatt gggatataaa ccagaatcgc aaaacccaaa
     7021 ccaatcgcaa gtattctttc actcccaatc ttgtcaccta agcgaccaaa tcttggagcg
     7081 gctatcaaag tggcaatccc aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc
     7141 ccatgtccat gcagcaattg gcgaatgtac agactgatga ttggactaat ggaattgttg
     7201 gatgcttgga tgatcatcgt tgtgacgaac ataccgatga cgacgtgtgg atatttcaag
     7261 tctttgaaaa tctgtttggc agaaaccatg ttttcttttt tcactgggac aaatttttca
     7321 tggacgaaaa acagactcaa caagaagacg aaaaataaga tgatccctgt aatgaaaaag
     7381 gtagggcgat agccaaacgc tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt
     7441 cctgtaacag aaccagttgc taaggttcca agaacttgac cacttttttc ttttggcgtc
     7501 ccagttgcga caagggcagt ggcattactg atataaccag aaaaaatccc ttgaagcaga
     7561 cgcaatgcga ctaattgata cacactagtc acgcaaccca tcaaagaaat gacgattgcc
     7621 attcctaatg aagcacgcaa cagcatcaat tttcttcctt tttggtcggc taattttccc
     7681 caccaaggag agataagcgt agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa
     7741 aagttcaatt gtgacgtact ataattacct aacgtatcta tgtaaagtga cataaaaggc
     7801 atgactaaac taaatccgat accagccata aatgttccaa accataagac aaacaagttt
     7861 tgttcccaag tttctctttt cctaaataat ttttccttta ttcttgcagc caattagact
     7921 ccttctttct gaaaattttc atctacagct ctttattata aactacaata tgtagtttat
     7981 caatgaaagt ctcttttttc tctttctttt tcgtggtaaa ctgaaagagt gaaggattta
     8041 ttttatgaga ggaaacatgg aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga
     8101 ctgctttcga gaactagcag aagagatgga tgttcagcaa ataacctttc agcatttagc
     8161 caaacgcttg gatatcaagg ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt
     8221 caaaacagcg ctaacagcga aacttctgca ggaacttaat gaccagctgc gacgggctct
     8281 tgtgggaaaa agtggtcagg aagcactgca gatctatgca gaaacttatc gatcctttgc
     8341 tttttcaaat caagcagtgt atgagttact gatcagtgta ccgcatacca atgaagctgt
     8401 tttattagat ggaatccatg aaacgaatca gatcattttg cagatatttg aggcttttca
     8461 attaacgaaa gaagaaaaag ttcatcgaag cagggaactt cgtagtatga tccacggtta
     8521 tctttcctta cgttttttag gatactttac aaaagaacca acaatcgatc cagaagacag
     8581 ctatttttgg atgatcaata actttatcgc tactttgcct taaacaatca gattcatttg
     8641 taacaatttt aagatgttca ttaaatgacc cttttataag agaaaattct tgcaactttc
     8701 ttgaaaagtg actaagatga gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa
     8761 gtaattacca tgttcgggga caatgagcct tggtggtttt ttgaagactg ggaagaagat
     8821 attgaagaag aagaaacatt cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa
     8881 tggaacgaaa tccatcaaaa ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc
     8941 ttttggaatg acggagatga gcgctggtgt gaagagtgtg acgatgattt acagcaatac
     9001 aagggacttg cgttgttgaa aaatcatcag cccatcacag tagaaagcag aaaggaattt
     9061 tatgaaacaa ctaattctag cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct
     9121 tggtgctaat caaaagcaca aaaattatta tgaaggaccc aaggtgatcg ttacttgggc
     9181 ccttggtcat ttattaggat tgaaaatgcc ggaagatctg aataaggaat ggcagagctg
     9241 gcaaatggac acgttgccaa tgatccctaa aaaactagga atcaaaccac ttcctaaaac
     9301 aggacatcaa ttaaaagcaa tcaagcagct ggctcagcga aaagatgtct cagaagcagt
     9361 cattgcgaca gatgccggaa gagaagggga acttgttgct cgctggatct tagagtatgt
     9421 gcatttcaat aaaccagtga agcggttatg gatctcttct caaacagata aagcaatcaa
     9481 aactggattc aagcaactaa gacctgcgaa agaatatgat gcattgtatg aatctgctct
     9541 tgcccgggca aaagcggact ggctggtagg attgaatgtg accagagcct taactgtgaa
     9601 gtacgcggat aatttatcag caggccgtgt gcagacaccg acactagcat tagtgagaga
     9661 ccaagaaaag aaaatcgagc agttccgtcc acaaacgtat ttcacgatca cgttgcaagc
     9721 tggaaaagaa caagccaaaa tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga
     9781 agcacaaaaa cttgttcaat tgatcagtac aagcaaagga actgtcacag aagtccaaga
     9841 aaaattaaaa acggaatctg cgccattgcc ttatgattta acagagatcc aaagagaagc
     9901 caatcaacgc tatggctatt ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga
     9961 gacccataag atcgttactt atccgcgtac agacagcaaa tatctgacaa atgacatgaa
    10021 aagtacgatg aaagaacgat tgcaagcagt cagcgacttt gctcctgagg tcaagcagta
    10081 tttgaaaaac ggagccatcg tccaacagac aaaagtattc caagacagta aagtgaccga
    10141 tcaccatgca cttttgccaa ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga
    10201 gcgaaaaatc tatcaaatga ttgtctctcg ttttttagga ctgtttgctc aaccccataa
    10261 agtcagccaa acaaaagtta cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa
    10321 tcgtgtcgtc caagcaggat ggaaaggcga aactgaaact gagacggaaa cagtcaaatg
    10381 ggaaaaagga atgagaatca atcccgattt cgccatcaaa aaagaactat ctgcaccgcc
    10441 taaaccattg tcagaagcaa gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc
    10501 agctacgcgt gcagagatta tcgagaaact aatcaaatct gaactgatgg aacgtacccc
    10561 tcaaggactg caagtatctc ctaaaggtaa gcaattatta gtattggtaa atccctcttt
    10621 agtaacacca gatttgacag aaagctggga aaaagacctt gaagctattg ctgcaggaaa
    10681 gaaacaagca ggaaccttct tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga
    10741 gatcaaatca agcaaacaag aatatcaaga tttctcgatt acccaaaaga aatgcccaga
    10801 atgtggggcc aatctgcgcg agaaaaacac acgtgacggc aaaatctatg tctgcaccaa
    10861 ttcagattgc aactaccgca gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca
    10921 atgtcaccgg aaaatggaaa tcatcgaagg taaaaacggt gcttatttcc gttgtaaata
    10981 cgatggaacg acagaaaaat tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca
    11041 tgaagaacga cgcttgctga aaaaatatgc acaagcagaa gaaccagaag aaagcccatt
    11101 agcagcagca ctaaaagcag caatgaagaa gtaataagat atgaaagagg ttgtgataga
    11161 agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    11221 aatttcggct tatttccgaa gaagctactt ctggaacacc atttattcgg aaaaaaggac
    11281 tgtgacaaga cttttgtcac agtccttttt catttaaaaa aatgtttact tcatttgacg
    11341 tatattattg gtgaggcttc aattcatttg gcttcacttt ttctgtttct cttatgatat
    11401 aaacaggacg ctgtttcgtt tctaagaaga ttttcccaat atatttcccg atgattccaa
    11461 gacataacaa ttggattcca ccaatgaaca ggaagatcga gaccatggaa ggccatcctg
    11521 atgtaggatc accaaacaac aatgcccgca aaataatgaa caaaattgca ataccagaac
    11581 cgacacaaga caaagcgccg ataaatgaag caatcgttaa cggtgcatcc gagaagttca
    11641 cgataccatc aatcgagtag ttgaataatt tccagaagga ccaagatgtt tcgccagcca
    11701 cacgttcgcg gttttcataa gaaatatatt ctgttttaaa tccgacccag ctaaataatc
    11761 cttttgaaaa tcggttgtat tctgttaatt ctaggacggc atccaccatt tgacgagtca
    11821 tcaagcggaa atcacgagcg ccatcgacca tttctgtatc gctgatttta ttgatcaact
    11881 ggtagaattt tttcgcaaag aaactgcgta tcgcaggctc gccttcacga gtcgaacgcc
    11941 gggtgccgac acaatcgata tcagaagtag tgatccggtt gatcatctca ggaagcagtt
    12001 cgggcggatc ttgcagatca acatccatca cggtcactaa atctcctgta gaagcttgca
    12061 atccagcgta cagagccgct tctttcccaa aatttctgga aaatgagaca aagcggacat
    12121 aaggataagc tgaggctagc ttcttgattg ctgctaacgt accgtctttt gatccgtcgt
    12181 caataaacaa atattcgaac tcatgggcac ctttgaattt ttcaacctca tcaaaaaaaa
    12241 gaggaacagt tttttcttca ttaaaacatg ggacaacaat tgatactttc atcgtttcta
    12301 tcctttcaat cgtatgttta tatatggttt acattcaaaa tttaataaac cttaaataac
    12361 acctttaaaa tttgatttta cgtaaaagaa caggtattct ttttgtaaac taacgtattt
    12421 agtttaccat gtttcctcgg tttatcaaac ttttaagaac aatttttatt agttggcaag
    12481 tagttttgga tagagaaaaa ggaggaagtt ccagtctatt gactaggagc aaacaaaatg
    12541 aaaatacaca cacctatcgg ctttttatgg ttagatgcag atgaatccgg attgactgct
    12601 gtatcttttc agccaatttc agaaagcgaa tcagcgaatc agcgaatttt aacgcgcaca
    12661 caagaacagt ttgaagaata ttttgcggga aaaagaaaag aatttgatgt tcctttatct
    12721 ataaagagag gtacgccttt tcagcaaaat gtctggaaag ctttgtcgac cattccttat
    12781 ggagaaatcc gaacgtataa agaagtagcc gttgctgttg atagtccgaa agctgtgcga
    12841 gcgattgggc aggcaaatcg aaccaatcca ttgccaatcg ttattccttg tcatcgagtg
    12901 atcgggcaaa atggaaaact gacaggatat atgggaaacg caatagatgg ggttgaaatc
    12961 aaacgccaat tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga
    13021 cattgttcca tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc
    13081 aaataaatag taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga
    13141 agcgtgtacc ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag
    13201 cagtgatctc aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag
    13261 aaaacaagct gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta
    13321 attttcgttt tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca
    13381 tgaatttttc aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata
    13441 gcatggcgaa ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa
    13501 cagattggag tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact
    13561 gtaatccaag aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga
    13621 tgatctgtcc tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag
    13681 aagaaccaca aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca
    13741 gttttttacc aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa
    13801 caattccttt tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa
    13861 aaacagagaa ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct
    13921 gtttaaaaaa agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta
    13981 actgtggcag ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta
    14041 aaccgggtaa gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg
    14101 atttcatttg atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc
    14161 actaaaccag tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct
    14221 tgtttcgaat aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa
    14281 aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt
    14341 acgacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta
    14401 caaaaacatg aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt
    14461 ttcacaacct attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg
    14521 ctatgttctt taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt
    14581 gcaaacagac attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg
    14641 ttgaagtaat gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag
    14701 gctgcatagc ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg
    14761 aaatacaata cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca
    14821 aagtttttaa gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc
    14881 ggacgtgtcg gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat
    14941 aagaacgcat agcagatcaa tgaaatccaa aagaaaattt ttccaataag tgggaagaag
    15001 ttcccggcag ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa
    15061 attgcttcaa ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg
    15121 atcacttgga agataacagc gcacagccaa atgaatttga caattggtgc aatactttca
    15181 tatgaaacaa aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag
    15241 gcgatgattg gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca
    15301 aaaatgaata tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg
    15361 tgatagtctt taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg
    15421 atcggaataa ttttcaaaaa gttcttcata tataatattc tcctttttat ttaatagatt
    15481 ttagtaccaa gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag
    15541 cagacagctg attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca
    15601 tttctattat agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat
    15661 aaaaagttat ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga
    15721 aacaagaaat ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa
    15781 tcaaatcaaa cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa
    15841 agagatgctt ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga
    15901 agaatgggaa gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg
    15961 gatcgttgct tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat
    16021 caaaaggttt cctaaagtgc attttgagat cgaaatcacg aattctcatg aatcattaga
    16081 acaagtagcg aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg
    16141 cgtacaaaga tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa
    16201 attgtggctt gtaagagaag caaactctgg tgtttttcat tacacgcagc gttattttga
    16261 agagatgaat atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt
    16321 gctgaaggaa ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc
    16381 aatgactccg ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc
    16441 tgatcagctg ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc
    16501 tcacagcgag gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat
    16561 acactaaaga taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga
    16621 aagttaattt ttcccaaatg tctatggaaa gactttccat tatctaactg gaccgctata
    16681 ataatcagca gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt
    16741 cgaacgataa tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg
    16801 gtctgctctc attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag
    16861 gcgtagggat gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc
    16921 atttgaattc caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg
    16981 taatgggact cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa
    17041 actggtttcg attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca
    17101 tcttgatgat ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga
    17161 aatcggactt ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga
    17221 tcatggccca agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt
    17281 tcctgatgtc tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg
    17341 ttggagcagg aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttataaag
    17401 ttggattcaa atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata
    17461 cgtcaggaat gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt
    17521 ttggtaaagg attcaacgta agcaatatct atgtgcctgt tcgtgagtcc gatatgattt
    17581 ttaccgttat cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt
    17641 ttattttaat ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct
    17701 atatcgcaag cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa
    17761 atatcggatt acttccttta acagggatcc cattgccttt cattagtcag ggaggatcct
    17821 cgattttagg gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag
    17881 cacctgcaag atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg
    17941 ttcaacatgc aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt
    18001 tgatatgatc aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca
    18061 agtaccaatc cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa
    18121 agatcaagta ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat
    18181 gctgatcaag cgaccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga
    18241 aaaaatttat gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca
    18301 acctttggat cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag
    18361 aagaacttgg agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag
    18421 atgaacctct gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa
    18481 cgggtacagt ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt
    18541 cagatgaaat gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg
    18601 aattataaaa tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa
    18661 tcataagcaa tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg
    18721 tttgaagaga gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta
    18781 gagcagagta agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt
    18841 atacggaaag aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa
    18901 tcaaggtggt aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag
    18961 gcttttttgt ttgaaaaatt ataagagaaa gatgtgaatg gcatgtcatt aatccaacta
    19021 acgaatgtca aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt
    19081 tcactaaacg ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa
    19141 agtacattag tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta
    19201 aacgatcaag atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc
    19261 ggtatgatct tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag
    19321 ctgccgctgg aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg
    19381 ttgcgtttag tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga
    19441 cagaaacaac gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt
    19501 gatgaggcta ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta
    19561 gcgatcaata agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc
    19621 cgcaaaatct gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac
    19681 gtattgactg tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac
    19741 atcgaaccag aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc
    19801 ggccgtttgg caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa
    19861 gcgattcgac gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa
    19921 gaaggttcct ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa
    19981 gcagaagtgt tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag
    20041 cttttcagaa acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat
    20101 ccttgataca ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt
    20161 tttgggattg atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta
    20221 tggaatcgta tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt
    20281 tttgatcatt ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat
    20341 tccagctctt gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg
    20401 tgaagtcagc cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat
    20461 catctggaag gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac
    20521 aacgatctcg atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg
    20581 aggtcttgct tggcaggaag gctaccagcg tggtaatcta acagtgacat tcgttgcgac
    20641 tttaattatt ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa
    20701 aacagacaaa agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga
    20761 tttgcagcag cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc
    20821 aaagattcag caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg
    20881 cacgcagaga ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa
    20941 atcgtcaaat tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat
    21001 gccaattact tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac
    21061 tttgtcaatg ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa
    21121 gatgtttccg aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg
    21181 ggacgcatca tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat
    21241 ctggaaacag caacattcga tgatattgaa gaaaacccta agaacttgaa gttcaaccat
    21301 acgatcgacc catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca
    21361 atcaacgcaa acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt
    21421 gaaaaggaca actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt
    21481 gacaaaatca aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca
    21541 gacaaatggg gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa
    21601 aaaagatgag aaataagtct gtaatggctt atttctcatc ttttttatac ctattctaat
    21661 ttagaaatca aaattgttta ataataaata aaagatattt gattttagta aggaaggtca
    21721 ttgcgcttca ttctcaatta gtctaaaatg agtgagaacg aaagagaatt tgggggtata
    21781 atgcatgtct gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa aagaaatcct
    21841 taaaggcgta aacctaacaa tgaaaaccgg agaaatccat gccatcatgg gacctaacgg
    21901 cacaggaaaa tctaccttat ctgccgctat catgggcaat ccaaactatg aagtaacaga
    21961 aggcgaaatt ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg aacgtgcgcg
    22021 acttggctta tttttagcta tgcaatatcc aagtgagata ccaggtatca cgaatgcgga
    22081 attcatgcgt gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt ctgttatggc
    22141 atttttgaaa aagttagatg aaaaaatggc tttactaaat atgccagaag aaatggctga
    22201 acgttatctg aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga tcctgcaatt
    22261 gttgatgtta gaacctacat ttgcaatttt agacgaaatc gattcaggtc tggatatcga
    22321 tgctttgaaa gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt tcggtgcatt
    22381 gatcatcacc cactatcaac gattattgaa ctatatcaca cctgatgtcg ttcacatcat
    22441 gatggaaggc cgagtagtca tgacagggaa tgctgaccta gctaaacgtc tggaagcaga
    22501 aggctacgct ggtatcagta aagaattagg aatcgactat aaagaagaag aagcgtaagg
    22561 gggagcaaga atgaaagaaa aaaactggct cgattatctt gacgcggtaa atgatttttc
    22621 tttaagtaaa ggcgaacctg attggatgag aaccttccgt caagacgcac tagcgaaagc
    22681 agatgaactg ccattgccgc atatcgatcg ggtgaaattc catcgctggt cgttatttga
    22741 tgtgaaagaa acacaaacca tcagtgaaac aggcacgatt ccggcatttg atgcaatgaa
    22801 agacaatcct gtgcttgtcc aacaaggttc atggacgatt tttgaacaac tgcctgtgga
    22861 attagcagaa aaaggagtca tttttaccga cttgttcact gcaatgatcg aatatccaga
    22921 actagtccaa gaatattata tgaaaaaagc agtaaacatg aacgaagacc aattgactgc
    22981 actccatgta gcttttatga acagcggtat tttcctgtat gtgcctaaaa atgtcgtgat
    23041 cgatgaacca ctcgagtcct tattcattca agatggcgca tccgatgagc acttcttcaa
    23101 gcatgtgttg atcgtggcag atgaacatag tgaattttct tatctggaac gtttccagac
    23161 aaccaaagaa caagtagcaa aatcatcagg taatatcatc gtggaagtca tcgctaaagc
    23221 tggttcaaaa atcaaatatt cagccgtaga tcaattagga gaaaatatta cttcttatat
    23281 gaatcgccgt gggcacattt tacgagatgc ttctgtcgac tgggcaattg gtgtcatgaa
    23341 tgacggtcat gtgatcgctg actttgactc agatttagca ggagaaggtg cacacgcaga
    23401 agtcaaaatc gtggcaatca gctcgggcag acaaatccaa ggaatcgata cacgagtaac
    23461 caataaagcc cctcatacga tcgggcatat tttgcaacac ggagtgatcc gagaaaaagg
    23521 aacattgacc tttaatggta tcggccacat cttgaaaggg gcaaaaggtg cagatgccca
    23581 acaagaaagt cgtgtcttga tgctttctga taaagcacgt ggtgatgcga atcctattct
    23641 tttgatcgat gaaaacgaag taactgctgg acatgctgcc agtgtaggac gagttgatcc
    23701 agaagaaatg tactatctga tgagtagagg attgcataaa gaagaagcag agcgcttagt
    23761 gattcgagga ttcctcggtt ctgtcttaac agcaatccct gtagaacaag ttcggaaaga
    23821 attagtcgaa gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa gctacgcaat
    23881 gattttccta tcttatttca agacgtcaat gatgaacctt tagtttatct ggataatgca
    23941 gcgacgaccc aaaagccaaa acaagtactc caggcattag cgtactatta cgaacacgat
    24001 aacgccaatg ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca tgattatgaa
    24061 caagcaagag aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga agtgctgttt
    24121 actcgtggta cgacgacaag cttgaattgg gtagccagaa ccttcggcga ccgctatatc
    24181 aaagcaggag acgagatagt gctctcttac atggaacatc attctaatgt tatcccgtgg
    24241 cagcagttgg caaaacgaac aggtgctgtc ttgaaatata tcgaactaac agaagaaggc
    24301 ttcctggata tggaggatgc agcgaaaaag attacggaca aaaccaaaat cgtctccatt
    24361 gctcatgtat ccaatgtctt aggtgtcttc aatcctgttc aagaattagc tgaacttgtc
    24421 catgaaaacg gcggggtcat cgtagtagat ggtgctcaat ctgccccgca catggccatt
    24481 gatgtccaag cactagactg tgatttcttt gctttcagcg gacacaaaat gtgcggacca
    24541 acaggtattg gtgttttata tggtaaacga aaatggttag aagaaatgga acctgttgaa
    24601 tttggtggcg aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa agaactacct
    24661 tggaaattcg aagcagggac accaaatatc gccggcgcta tcgcattagg ccatgcaatc
    24721 gattatttgc aaaaactcgg tatggacaat atccatcgat atgaagaaga actagccgct
    24781 tatgtgttgc caaaattaca agcaattgat ggattgactg tttatggtcc acaagacccg
    24841 aattttcgga caggagtcgt tgcattcaat ttggatgggt tacatcccca tgatgtcgcg
    24901 acagctttag acatggaagg ggtagccgtc agagcaggtc atcattgtgc gcaaccgcta
    24961 ttgaagtacc ttgaagtatc agcgactgct cgcgcgagct tttatttcta caacacaacg
    25021 caagacgccg accgtttagt tgaagcaatc atggcaacaa aggagttttt ccaacatggg
    25081 actatctaaa ttagacaatt tataccgtca agtgatcttg gatcattctt cccatccgca
    25141 tcatcgtgga agcttgactg actcgacaag caagatcgaa ttgaacaacc ccacatgtgg
    25201 agatgtgatc gaattgcaag tcgatgtaaa agacggaatc atcaaaaata tcgcctttga
    25261 cggcagtggc tgctccatca gtacggcaag tgcctcgatg atgacagacg ccgtcatcgg
    25321 aaaatcaata gaagaagcag aacatttaac tgtagacttt ctacagcttg tccaaggaaa
    25381 agaagtcaaa gatcccgacc aattaggcga tgctgctatg cttggtggtg tagctaaatt
    25441 tcccgctcgg atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag caatggaaaa
    25501 cgatggtacc gcagctttcc atcattacca caaggaagag gagtgagaac atgggcgtac
    25561 cagaattaga agaatataaa ttcgggtttc acgatgatgt cgaaccggtt ttcagtacag
    25621 gagaaggctt gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc gaaccagaat
    25681 ggatgcttga attccgtctg aaatctctag aaacatttaa caaaatgcca atgcaaacat
    25741 ggggccccga cctttcagac atcgatttcg atgccatcaa atattaccaa aaaccaagtg
    25801 acaaacccgc tcgtgactgg gaagacgtac cagaaaaaat caaagataca tttgaaaaga
    25861 ttggtatacc agaagctgaa cgagcttatc tagccggtgc ttccgcacaa tatgaatcag
    25921 aagtcgtgta ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc tttactgaca
    25981 ctgactctgc tttgaaagaa taccctgaat tattcaaaga gtacttttca aaacttgtac
    26041 cgccaaccga caacaaatta gcagcgctga attcagccgt atggtcaggt gggaccttta
    26101 tttacgtgcc aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc cggatcaatg
    26161 cggaaaacac ggggcaattc gaacgtacgc tgatcattgt tgacgaagga gcaagcatcc
    26221 actatgtcga aggttgtacg gcacctactt attcaagtaa cagcctgcat gccgccatcg
    26281 tagaaatctt tacaagaaaa gatgcgtatt gccgttacac aacgatccaa aactggtcag
    26341 acaatgtgta caacttagta accaaacgag caaaagcata cgaaggcgca accgttgaat
    26401 ggatcgacgg aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt tacttagatg
    26461 gaaaaggtgc acgaggcacc atgctctcca tcgcctttgc aggagcaaac caaatccaag
    26521 atacgggtgc aaaaatgatc cacaatgcac caaatacatc tagctctatc gtctctaaat
    26581 caatttcaaa agacggcgga gaagtaaact atcgaggaca agtaaccttt gccaaaaaca
    26641 gtgcgggttc gatctctcat atcgaatgtg acacgatcat catggacgaa tggtcgaaat
    26701 ccgatacgat cccattcaat gagatccaca acagccaagt ctctttagaa cacgaagcca
    26761 aagtctcaaa aatctcagaa gaacaactct actatctgat gagccgaggc ctgaccgaat
    26821 ctgaagcaac tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa gaacttccaa
    26881 tggaatatgc agtcgaactc aatcgattga tcagttatga gatggaaggg tctgtaggtt
    26941 aataactatt tcattgctcg aaacgttgat attaaagcgt ttcgagcgtt
//
LOCUS       DACEZN010000020        25763 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEZN010000020 DACEZN010000000
VERSION     DACEZN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.020"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(107..1405)
                     /gene="eno"
                     /locus_tag="IXH60_000662"
     CDS             complement(107..1405)
                     /gene="eno"
                     /locus_tag="IXH60_000662"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1989627.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1521..2276)
                     /locus_tag="IXH60_000663"
     CDS             complement(1521..2276)
                     /locus_tag="IXH60_000663"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1989628.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYETGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2380..3570)
                     /locus_tag="IXH60_000664"
     CDS             complement(2380..3570)
                     /locus_tag="IXH60_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1989629.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3680..4681)
                     /gene="gap"
                     /locus_tag="IXH60_000665"
     CDS             complement(3680..4681)
                     /gene="gap"
                     /locus_tag="IXH60_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1989630.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4722..5759)
                     /locus_tag="IXH60_000666"
     CDS             complement(4722..5759)
                     /locus_tag="IXH60_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1989631.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(5962..7296)
                     /gene="rpoN"
                     /locus_tag="IXH60_000667"
     CDS             complement(5962..7296)
                     /gene="rpoN"
                     /locus_tag="IXH60_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1989632.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7356..7427
                     /locus_tag="IXH60_000668"
     tRNA            7356..7427
                     /locus_tag="IXH60_000668"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7389..7391,aa:Arg,seq:ccg)
     gene            complement(7619..8503)
                     /locus_tag="IXH60_000669"
     CDS             complement(7619..8503)
                     /locus_tag="IXH60_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1989633.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8590..9489)
                     /locus_tag="IXH60_000670"
     CDS             complement(8590..9489)
                     /locus_tag="IXH60_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1989634.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9506..10012)
                     /locus_tag="IXH60_000671"
     CDS             complement(9506..10012)
                     /locus_tag="IXH60_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1989635.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10201..10821)
                     /locus_tag="IXH60_000672"
     CDS             complement(10201..10821)
                     /locus_tag="IXH60_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1989636.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10897..11334)
                     /locus_tag="IXH60_000673"
     CDS             complement(10897..11334)
                     /locus_tag="IXH60_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1989637.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11497..12327
                     /locus_tag="IXH60_000674"
     CDS             11497..12327
                     /locus_tag="IXH60_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1989638.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12311..13678
                     /locus_tag="IXH60_000675"
     CDS             12311..13678
                     /locus_tag="IXH60_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1989639.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13697..14506
                     /gene="yidA"
                     /locus_tag="IXH60_000676"
     CDS             13697..14506
                     /gene="yidA"
                     /locus_tag="IXH60_000676"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1989640.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14549..14992)
                     /locus_tag="IXH60_000677"
     CDS             complement(14549..14992)
                     /locus_tag="IXH60_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1989641.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15126..15497
                     /locus_tag="IXH60_000678"
     CDS             15126..15497
                     /locus_tag="IXH60_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1989642.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15666..16448)
                     /locus_tag="IXH60_000679"
     CDS             complement(15666..16448)
                     /locus_tag="IXH60_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1989643.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16445..17416)
                     /locus_tag="IXH60_000680"
     CDS             complement(16445..17416)
                     /locus_tag="IXH60_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1989644.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17508..18902
                     /locus_tag="IXH60_000681"
     CDS             17508..18902
                     /locus_tag="IXH60_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1989645.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEI"
     gene            complement(19094..20449)
                     /locus_tag="IXH60_000682"
     CDS             complement(19094..20449)
                     /locus_tag="IXH60_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1989646.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(20479..21114)
                     /locus_tag="IXH60_000683"
     CDS             complement(20479..21114)
                     /locus_tag="IXH60_000683"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1989647.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            complement(21121..22497)
                     /locus_tag="IXH60_000684"
     CDS             complement(21121..22497)
                     /locus_tag="IXH60_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1989648.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            complement(22490..24271)
                     /locus_tag="IXH60_000685"
     CDS             complement(22490..24271)
                     /locus_tag="IXH60_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1989649.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            complement(24293..24604)
                     /locus_tag="IXH60_000686"
     CDS             complement(24293..24604)
                     /locus_tag="IXH60_000686"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1989650.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            complement(24594..25580)
                     /locus_tag="IXH60_000687"
     CDS             complement(24594..25580)
                     /locus_tag="IXH60_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1989651.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            complement(25595..>25763)
                     /locus_tag="IXH60_000688"
     CDS             complement(25595..>25763)
                     /locus_tag="IXH60_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1989652.1"
                     /translation="NNELPFMMVLSDETVADQAGFLINDQGVQYNFLFSSLVQDIQGT
                     MSFEIANQLFE"
BASE COUNT         7255 a         5487 c         4568 g         8453 t
ORIGIN      
        1 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
       61 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
      121 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
      181 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
      241 ggccagcatt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
      301 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
      361 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
      421 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
      481 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
      541 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
      601 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
      661 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
      721 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
      781 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
      841 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
      901 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
      961 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     1021 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     1081 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     1141 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     1201 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     1261 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     1321 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     1381 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     1441 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     1501 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaaaaa tgaatctgct
     1561 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     1621 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     1681 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     1741 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     1801 acttgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     1861 gttttgcctg tttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     1921 tttgcaaaga tagcttttgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     1981 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     2041 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     2101 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     2161 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     2221 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     2281 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     2341 ttgatcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     2401 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccaccac ctgttgagat
     2461 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcagctgagt ctccgccacc
     2521 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     2581 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     2641 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     2701 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     2761 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     2821 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     2881 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     2941 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     3001 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     3061 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     3121 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     3181 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     3241 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     3301 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     3361 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     3421 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     3481 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     3541 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     3601 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     3661 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     3721 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     3781 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     3841 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     3901 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     3961 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcaggaa
     4021 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     4081 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     4141 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     4201 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     4261 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     4321 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     4381 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     4441 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     4501 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     4561 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     4621 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     4681 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     4741 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     4801 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     4861 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     4921 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     4981 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     5041 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     5101 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcacg gtggttaccg
     5161 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     5221 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     5281 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     5341 ggaagtagtc ggtctagggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     5401 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     5461 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     5521 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     5581 tttttcaaaa gatctgtttc tgttcgcaat actcgttctg tgatccccat actttccgat
     5641 aaacttcgac gtccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     5701 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     5761 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     5821 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     5881 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaatac
     5941 tttttctagt cttttgactg attagttatc ataacgtttt ctttttgatg aactcgggat
     6001 tcctaataaa tcacgatatt ttgctactgt tcgtcttgag atcgctatct catctttttt
     6061 cagcaattca accaatttct ggtcagacaa cggcttgttt ttgtcttctt gatcgactaa
     6121 ttcctgcagc tttttcttcg ctgtatctgc tgaaaggtct tctgtctgtt ctgtactatt
     6181 cgtaggaatt cgtgtagtga aaaatttttt taattcaaat acaccgaagt ctgtttccaa
     6241 atatttcccg ttgactgcac gactgaccgt ggattcatgc acatctatct cagttgcaac
     6301 ttcctttaac gtaagaggct tgattggacg ttccttgtcg ataaagaaag ctttttgccg
     6361 ggaaacaatg acttgagcaa cccgcaaaat tgtatcccca cgttgtagga tcgttttctt
     6421 caaccattcg aattcctgtt tccgttcttt taaataattt tcagtttctt tatctgcttg
     6481 ttgtgacatt tgtttgaagt aactctcttg aaagcgtata tttggctgat tttttcggtt
     6541 tgagataact tgtacatttt tatcgtcgtc cactaatacc cggacatcag gaataatgta
     6601 caattcgctg ctacggtcga aaatccggcc agggcttgga ctcaacgttt gtaaatagtc
     6661 gaatatctgc tgaacagcat gtagatcaac agaaaaacgt tgtgcgattt ctttccattt
     6721 tcgctccacc agttcctcaa aacattcttc caacacgata taagctaatt ccggcgcata
     6781 atcatctcgt tctgtttgaa gcatgagaca ttcttgcaaa ctacgtgcac ctactccagc
     6841 tggttctaat tgctgaatca gagtcaacgc atcgagcatt tggataggcg tagctccaat
     6901 ctcttttccg gcttcttcta aactaatcgt caaaaatccg ttcaaatcga tgtattctac
     6961 aagataaaga acgattttcc tcaaaaaagt atctcggtaa ttcaaatgga cttgttcgat
     7021 caatgaatca aataaagacc cttttgtatc aggaatctga tttaggtaat ttgtctcttc
     7081 tccttcataa gaagatgaag aagcttttat gtaatctggc tgccattccg gctcaacaac
     7141 ttccaccaat ggattttcca ttgcttgatt ctcaacgaat gccaacagct cctcactgtt
     7201 atattgtaaa acttggatgg actgttgcaa ctgttgcgtc atggctagtt tctgtgtctg
     7261 ctgctgtttt tgtgaaaaat tttgctcaaa tttcattttt ttgtccaatc cccttgtaat
     7321 tttttatctg tttagttaca attatcccag tgcttgcgtc cttagtgtag tggatatcac
     7381 gtaagattcc ggttcttgag acgggggttc gattccctca ggacgcgttc tactgctatt
     7441 ttagcataat ttacgaatag ttgcttcttt atttttaagt gaaaactgtc cgccaaagaa
     7501 aattggtgga cagttttttg ttgtacctgt atagatattg ttaagccttt gcaagtaagg
     7561 tagttatgtg ttcaactact tattaaaatg tgttatttat taaagatact tgtctcactt
     7621 actgaactgg ccgatgatcg tttctgccat aatagttctc gacagccgtt ggatgcccta
     7681 ataaatcccc aactgtcgtg atgcgatacc cttgctcttt cagataatcg atcacttgag
     7741 gaacggcacg aatcgtttca ggatagatat catggaataa aataattgcc ccatcgtata
     7801 ctgttgcttg gatcttctgc aaaatcaaat caggatttct tgtcttccaa tcttctgaat
     7861 caacagacca ttcgatgatc ggtcgctgga tgatcgttgc tcctacattc gtgacagaac
     7921 catagggcgg acgaacaaaa gtgggggttt tgccagtgac ttttttgatc tcagtgtctg
     7981 ctttcgtcac ttcctccgtc atcgactggg cagaaagagt ggccagattc tgatgatcgt
     8041 atgtgtgatt gccaatctcg tgccctgctt cagctgtctg cttcacgatt tcaggatgct
     8101 gcgccgtatt ttctcctaag acgaaaaaag tggcatgcac gtttttttct tttaagattc
     8161 gcagaagctg cggtgttgtg gtcgctcctg gtccatcatc aaagctgatt gcgacgcttt
     8221 tgacagccgg atctcggctc gtttttgtac tgtcttgtgt tgattgtgat gactgacttt
     8281 gtaccttttc cgctgattct tgaatggctt ctttgacgtt agctgtttca gttgattggc
     8341 ttttaatggt tgattgttct gattgtttga aagattgtga cgaatgctcc gaagagtgtg
     8401 tttgtccttt tacttcggtg atcaaggagg ccaccgaagt aatttcgaaa aacaaaagca
     8461 cggataaagc aataatactg ataactaaat aatttctttt cactatatct cttctttctc
     8521 tttttctctt cttattattt acttgtactt acttgaaaat tgaaactgct agtcgcaact
     8581 acttctcgtc taatgatcca aataatcttc cgatgccaaa atcgtttttg tcacggactt
     8641 aggaatgaca aattctggtg tacccatata accaggagca atttcgtatt gagaaaaagt
     8701 caaaactaat tgatgctctt tgttcaaata aaaacttcgt gtaggcgtaa caagtgttgg
     8761 tttttctatc gtcccttctt gaatatcttg ctcagtccaa tagtacttag atggatcttc
     8821 cttgacttgt tcggccatct gccgtttgac ttcttttgtc aaaacagata gataggtgtc
     8881 atctttgaat aaaagtggaa gtgagagctg gacactgttt ttcttgtcga ttgtgtcgta
     8941 tcttttttca acatggctat ctgctgctgt ttgggtgatc tgcctttcaa tcacgagaaa
     9001 ccggttatcg tctacgattt tctgataatc tcccaaaact tggtaatgat ccgtttcaaa
     9061 atttccatat tctttttcag ctttttcaaa atctgcttgt ccttcgcgaa agtatttttg
     9121 atttaatcca tgaattgttt tattttcttt ctcattttgt gtattgatct tcggtacctg
     9181 aatatcgatt ttttcagatc gatcagtaaa gcgttcgcct aagatcaatt caatgactga
     9241 tcctaagatg ggtaaatctt ctgcaaagct tcgaacttga ctattaaaaa gaaggctact
     9301 tgttgtaatc attacgatag ccaaagatac tccgaaccct tggagacgtc tctttctccg
     9361 ctttgctttt ataaatgatt gctcttcgat aataaaacgc tggcggatct cctctttaag
     9421 ccctttggga attgcttgtt ctttataagt tttctttagt ttttttaaat tttctttttg
     9481 ggaatacatg gcactcttct ctccttcatt ctaggatttg acgtaaagca ttcaatgagc
     9541 ggtacatttt cgttttaact gtgttttcat tcatatccaa gatagtcgca atttctttca
     9601 aacgaaatcc ttcaaaaaag tagaggatca gaatctcttt ttccggactg tctattttat
     9661 ctaaaatcat atggagctct gcaacggaag tttctaaagg taactgttgg gggatatctt
     9721 gaatttcaaa taagtccaca ggcgtccttt tttgtctgcg ccaactatct aatgccgtgc
     9781 ggatcaatat ccgataaaac catgcactaa aatgctcgat ctctttttct ttttcaaaat
     9841 agttcagggc tttatgaaaa ctatcttgca tcacatctaa ggcctcttct ttatcatgta
     9901 cgtaattcat cgtgatacgg aacatttttt gataatcagt ttcgatgagt ccattcagca
     9961 ggtgacggta tttttcgatc tgccgttttt tcttatatcc agtaaaaatc attctccact
    10021 cctttctgcc tcacttaata tgacgtacaa ggtgcgctaa aagtttcaat ttttttattt
    10081 tttatgtatt tacttgggat ttttatatac aaaaaagaag gtgtgacaaa aatcgtgtca
    10141 cacctcctta ttctgatagg ctgatcgtct ctgtcacaac ctctcgtaag aaaataatgt
    10201 ttattctttt tcagagtcag aaaattcttc ctcttcataa tcttcatcat catcgtcttc
    10261 gtctatgata cttaaatctt cttcgatatt tcctggcaag tcttcttcat catctgtcgg
    10321 gtcatctgct ccgatttctt gcaagtcaga attataagca gcgatttctt catcttcatc
    10381 atcatcgtca tcaaacagat catcgtcatc ttcttctgtc agatctgtat cttctgggtc
    10441 atcgtcattg taatcgatcg cgtcttcatc atcattgata aaggcgttga ctttcttgcg
    10501 tttacggcga cgtggagtat cttcatcctc ttcgtccatt ccatggttta cttcttcatc
    10561 gatcgaatcg atagcatacc atgaacgcaa gccccaacga ttatccccta atgagataaa
    10621 gctgccgtca atattcaagt cagtataaaa ttgtgcgagt gaatcacgaa tttcactgtc
    10681 tgatttccca agatagtttt ggatctggtt agctaaatca gaaaaatcca tgacatcgcc
    10741 acgttgttct aagatcgcgt gtgctacttc gatcattgat aattcgcttt tgtttgcacc
    10801 ttcaaataca ctaatttcca atcaggacac gtcctttcac agtctactcc ttatcataca
    10861 aaatcagaca caaaaaatca aacttttctt ttatatttac gctgtaaaat ccaaggtcaa
    10921 ataatattcg ccgatttttt ccggtcccat gaacagatca taatcgattc gaaccttccc
    10981 tgctgtggga cggtctttca aactaacatg cagttctttc gtaaaagtag caatctggat
    11041 cataccgtat ggtgttcgat aggatgtttc taaccgttct ttatagccaa atttcaaacg
    11101 catccgcatt tctcctgcac ggatcagctg tacatgacca tctggaacta atttgatcgt
    11161 tacaggaatc tcatcgcccg tctcctcctg aatctcttta taacgtatgt acagcgtatc
    11221 gccaattgtc accatttgcc ctttcaaatc aaaataaaaa tcttgcactt ccccttgctg
    11281 ttttactttt gtacgcaatt tgattgaaat cggtgttcct tgtgataatt ccattattca
    11341 ttctccttta cttctcatag ctacattgtt cctagtttct tttgttttgt caaccaactt
    11401 gtccattttt acagaaaacc ttttttccaa aacaactctt tgattttaga gtataatatt
    11461 gtagaaaata tgaatttata caaaggagcc ggttgaatga accctgcgat tcgactagat
    11521 cagcacattt atcaaaaaaa cgacttttcc ccttttgcat taacagatat attaacggaa
    11581 tattctaaag aacaccagca aatcttttta catttctggc aatatgactg cacagtcatc
    11641 ttaggtatga aggatgttcg aactcctttt ctaagtgacg ggatccaaac actggaagat
    11701 gcaggttacg ctccagtgat cagaaattca ggtggattag ggatcgtatc agatgaagga
    11761 attttgaata tttctttgat tttcccccaa agtgacacaa aaaaaataac catagatgac
    11821 gcttacgagc aaatgctcct attgactaaa tacgcttttc caaaggcacc gatacaagca
    11881 ttcgaaatca cccattctta ttgtcctgga acttatgatt taagcatcca tggcaaaaaa
    11941 tttgccggta tcgctcagcg tcggatcaaa aatgggattt ctgttatgat gtacttaagt
    12001 gtcaacggta atcaggaaaa acgtgggcaa gtcatgaagc gattttatca atctgcttta
    12061 aaagaagact ttggtacaaa tggctatcct gcagtggatc ctgctacaat ggcaacactt
    12121 gaagaactgc ttgaccaacc cctttttgta aaagaagtca aagaagcatt tgcaaaagct
    12181 tttgaaaagc tgtattctgc aagtgattta caggattcta ccgactggct aaaaaaagaa
    12241 atccagctgg agcaatggaa caaccaaatc cagagaatgg aacaacgtaa taacataaag
    12301 gagctacatt atgacaaccc cttataaata tcaaatgctg cctatggaaa aagtttttcg
    12361 tgatccagtc cataactata tccatgtcca gcatcaagta atcttagatt tgatcaattc
    12421 gaaagaagtc caacgtctac gacgcattaa acaattaggc acttcttctt tcacttttca
    12481 cggagcagaa catagccgtt ttactcattc attaggcgtt tatgagattt caagacgaat
    12541 ctgcgatatc ttttctcgaa atttttcaaa agaaaaaata ggcaacggag gctgggatga
    12601 tcaggaacgg ctggtgactt tatgtgcggc actgttgcat gatgtcggtc atggtcctta
    12661 ctctcataca tttgaacata tcttccatac gaatcatgaa gccatcactg tagagatcat
    12721 cacttctcca gaaacagaag tgtatcagat tcttaaccga gtagaagaag gttttcctga
    12781 aaaggtagct agtgtgatcc aaaaaactta ccccaaccca caagttgtcc aaatgatttc
    12841 tagtcagatc gatgcagatc gaatggacta tttgcttcgg gatgcttatt tcactggaac
    12901 agaatatgga acatttgatc ttacccgaat attacgtgtg attcgtcctt atgaaggcgg
    12961 gatcgctttt tccatgagtg gcatgcatgc agtggaagat tatatcgtta gccgatatca
    13021 aatgtatgtg caagtgtact tccatcctgt ttcccgtggt atggaagtaa ttttggagca
    13081 tctgcttcat cgcgcacacg agttgtatca agaagatgcc agcagttttg aactccattc
    13141 tcaattactt ctgccatttc ttgacggaag ctttacttta gaagaatacc tcaaacttga
    13201 tgatggggtc ttgggcactt actttactca atggtctgaa tctgctgatc ctattttaaa
    13261 tgatttagct aaacgttttt tgaatcgtaa accactgaaa tctgctactt tttctggcaa
    13321 ccgggattca aaattagtcc aagaactcac tttgttagta gaaaaggtcg gatataatcc
    13381 tgtttattac actgctgtga attctagcta cgatctacct tatgattttt atcgcccaga
    13441 acaaggcaga catcgcacac aaatcgagat tttgcgaaat gacggaactt tgatagaatt
    13501 atcacaggtt agtcagcttg ttgctgcatt agcgggacaa gaacaaggag atgagcgatt
    13561 tttctttcca aaagaaatgg ttgatccgtc cttgcgtgat cattatgatt tatttgacga
    13621 aacttatcaa gaatttgcca gccatatccg taatggcgca ttgattgaaa taaattaaaa
    13681 tatattggag gaaattatgt ctatcaaact agtcgctatc gacatcgatg gtactttatt
    13741 aaatgagaaa agagaagtaa caaaagaagt aaaagaagcg attgccgctg ctgttgcaaa
    13801 aggtgtttcg atcgttttat gcactggccg tcccttacca ggtgtccagg aacagttaaa
    13861 cgagttgaac ctttttcaag ataatgatta cgttattact tataacgggg cactcgtaca
    13921 acaaacgaaa tcaggtaaaa tcatcgcccg tcacggactg acacatgaag atttcctaga
    13981 gatcgaagta atggcacgcc gtgtcggcag tcatctccat tcgatcgatg acaaagcgat
    14041 ctacacgcct aatcgagata tcagcgctta tacgatccac gaagcaagtt tagtcaagat
    14101 gccgctaaaa tatcgaacac ctgaagaaat gacaccagac atgaacatcg tcaaaatgat
    14161 gatgatcgac gaaccagaaa ttttagatgc agcaattgcg cgtttaccgc aaactttccg
    14221 tgataaatac acaacggtca aaagtgcgcc ctattacttc gaagtactaa ataaagaagc
    14281 aagtaaaggt gcggcagttg cgaatctcgc acagcacctt ggtattgatc aagatgaaat
    14341 catggctatc ggagataacg agaacgactt atccatgatt gaatatgctg gtctaggagt
    14401 agcaatggga aatgccgttt ctcttgtcaa agaagcagca aatgtcatca cttcatcaaa
    14461 cgacgagcac ggtgtagctg aagcaatcaa aaaatatgta ctgtaataaa taaaaaaact
    14521 gagagaaagc tgatctactc gctttctttc agtttttatt tttttcgatc catctctcga
    14581 ctgcccatcg gtggctacca cgtttcaatt tcacaagcgc ttcttgaggg ctcacccacc
    14641 aaattttatt ggtcttttca gttggttctc ctatacgctt ccaagaatca gcaacaaaga
    14701 aatatcctgg attataataa taagttgcac gataattcga gtaaaaatac tcatcagctt
    14761 ctcccagata agaagaaatt tctgccacaa ttcccatttc ttctatcagc tcacggacaa
    14821 tcgcttcttc tttcgtctct tctccttcaa tttccccgcc aggaagaaaa aaagcaccat
    14881 ttggagcctc gattactgca atctgctgat ctctttctat gacaatatat gctgcataac
    14941 gggtttgata atcagcaaat tgcttttttt caccaaaaat tggtttatcc atgaatagtt
    15001 tccaccttaa cagttttatt ttctatccat tttacatgag aaatttctca tgaacaagca
    15061 caaatcaact atttccttac ttctttttgt tatgattgag taaaaggaaa aggagagatg
    15121 aatctatgaa aatggctcac acatgtgttc gagtgaaaga tcttgaagca tcaatcgaat
    15181 tttatcaaaa ggcatttggt tttgaagaaa gccgccgccg tgattttcca gaaaacaaat
    15241 ttacacttgt ttatttgaca cttcctgggg atgactatga attagagttg acttacaact
    15301 atgatcatgg tgcgtatgat ctaggagacg gctatggtca tatcgctata gcagctgatg
    15361 atctagaaaa attacacgag caacataaag ctgctggtct tgagattact gacttaaaag
    15421 gattacctgg cactgcgcct tcttactact ttgtcattga ccctgatggt tataaaatcg
    15481 aagtcatccg aggatagtta gtatggggca ttttactctt gattgaataa aaaacaaaca
    15541 ccaataatta aaatgagacg caaacttttc attaaattga aaaagtttgc gtcttttttt
    15601 aacaaccatt gtagctataa ggttgccact ctccaccaat ttatttttaa ttcatttgaa
    15661 agaaatcaaa taaacgaggt acctttagaa aacaaaactg cttttcctcc tattatcaca
    15721 cgatcttctt ttaattcaca ttctagatcc ccgccccttg atgacatttg ataagctgac
    15781 aattttgttt tattcattct ttctgcccaa taaggaataa gattagaatg tgctgatcca
    15841 catactggat cttcattgat tgttaatttt ggaaagaagg ttctagaaac aaagtctttt
    15901 tgcacacttt ctgctgtcac gatgactcct attcctaatt caaaattttt gatagctgta
    15961 aaatctggct gcaatttagc caccgttttt tcatcttcta atacgaaaaa taaatcacgt
    16021 gctaagtagg cttctttaat cttagcccca atcgcttctt catattcaga caatatcggc
    16081 acttttttcg gcataatact aggaaaatcc atgtaataat aatctttaat tcttgtcaca
    16141 aatagattac cgctttgagt agaaaattta atcgtttcat caggtatttt gtaatagtta
    16201 aataaaacaa atgctgttgc taatgtcgcg tgaccacaaa gatcgatttc tcgatctggc
    16261 gtaaaccatc taagctcaaa ttcttggttt ttcttcactg taaatgctgt ttcagataaa
    16321 ttgttttcaa tggcaatttt ttgcatcgta ccttctggca gccattcttc tagcacataa
    16381 accgctgctg gatttccttt gaacacctca tctgcaaatg catcaactac gtaataatct
    16441 aagttcattt taaagcccct ttctctaatt tgttataaat atattctgca caacaaagat
    16501 cttcaatagc tagaccaact gcgtcgaaaa tcgtcggcct cgattctcct ttataaaatg
    16561 tttttttctc agaaactaat tcgcctaatg taccaacaat acaagattcc tctaaatctc
    16621 cttctgccat ggctataagc aggtccccgc tttctgttaa ggcagctgag taatcatcta
    16681 catagatttg agaagtttcc atcaacgatg tacttatctc tcttgtatca ggagtaaatg
    16741 tgcccacagc attgatatgt gcgtcttccc ttacccactc tttttgaaga aatgcctctt
    16801 tactagaggt caacgtacaa atgatttccg ctggacgtac tgcatcttct actgatattg
    16861 catttataaa cgttatttta ggatactgtt ttttagcttt agcgataaaa gtatttcgtc
    16921 tttcttctaa aatatcgtaa acaaacactg tagtgatggg acgaatggta gaaatcgcag
    16981 tcaaatgcga caatgcttgt tgaccagctc caattaaagc aagtgtttct gcatgcggat
    17041 ttgctaagta atctgttgcc agcgcagata cagcagctgt tcgaatccag gtgactgcat
    17101 ttgcgtttat caatgctttg ggtagtccgt tttgagcatc aaacaagaaa atctctccta
    17161 aatgagaagg taaatgatac tgatgattat ccggaaaagc ggtaatgact tttgtgccaa
    17221 aatatctgtt ctcccccaaa taagcaggca tcatcgcaaa catatttttg ccttttccgt
    17281 caggtaaaat ttccaccaat cgaggatttt ggctgatttt accactttca taatcaaaaa
    17341 aacatttttt cattacttga atcgcttctt caaaactaaa agaagctact acttcctcaa
    17401 aattaataat tttcatagac aattccctcc tcacaaaaga ttaacttcta aaatcaatac
    17461 aatcaatggt aaaattgtat ggtatacaat tttaaggagt ggagaaaatg cctattaatt
    17521 cttatgacaa ttacccgtta acttggcgac cagacaaaca aaaattgaag cgtccttatt
    17581 atcgatcgtt gattcaacaa ttagaacaag atattttaaa gaataaatta cagaaaaaca
    17641 ctcagttacc ctcccaaaga gagctagctg attatttaga tttaaatttt actaccgttg
    17701 gcaaggctta taaatatgga attgagaaag gtttgctgta tacgaatatt ggaagtggca
    17761 catttatttc tcccaatgtc tttgagtcca tcaccatctc taccaatgaa gtaaccgaac
    17821 atatcattga ttttggtctg gttagttctt ttgaagaatg taataaattc gttgtcccgt
    17881 ttattcaatc aactagtaat aacccaaatc ttgttggttt gcttaattac caagatccca
    17941 aaggcacaaa ttttcaattg actactgcag caaattggtt aaaaactcaa ggagtcattc
    18001 cgtccttaga atctctatct attgtttccg gtgttcaaaa tggattggct atcacacttt
    18061 ctgcactttt ctctccaggt gatagaatag caacagaccg atatacatac gctaatttca
    18121 ttgaattggc tcagttatat catttagaga tcgttccaat tgattttgac gagcaaggaa
    18181 tgcgacctga tatattgatt caggaatgta gaaaaaagaa gattcatggt gtctttctta
    18241 tgccttcttg taataatcct atagggtttc aaatctctca caaaagacga gagcaattag
    18301 ccgaagtgat tgaaaaagaa ggtctatggg ttattgaaga tgatatccat tcatttttaa
    18361 caacttacta tcaacatacg atcattcctc ctttccaaaa tttattacct aaccaaacaa
    18421 tctacttagc tggaatgaca aaatttattt gttctggatt gcgagttgct tatcttgtag
    18481 ttccagtaca tgtccaaagc aagatagaac aagcaatttt taatataaat gtgaaaactt
    18541 ctggattaga tgtggaagtc atcactcaaa tcctccattc aaatgaagca aataggatct
    18601 tagaaactaa attaagtctt acaaaaagag ccaatcagct ttttgattct cttttcaaag
    18661 tagaaaaacc aacgaatcgt gtcccttact atcgaattct tccagttgat cctgagattg
    18721 accctaagat aattgagaat gactttcttg ctaacggtat ccgactttat cattccagac
    18781 gctttactac acgtgcgcaa ccagatgcat tcgttcgaat ttccctatcc tctaataacc
    18841 ttgaaatttt agcttctgga ctaagtatcg taaaagaaaa aataaaaaaa tatgaaatat
    18901 aacctattga taaataaagg aatcaaatct tattgattcc tttcaataaa aaagaggcac
    18961 tacccgaggt tcaacaacgg gtagtgcctc aaaaaataaa cgaacatttt gcacttaaag
    19021 tatccaagaa gcaagaaaaa ttattttatt ttcttatttt actaaatcac taggtttctg
    19081 tcagaagatg aaattatcca atcatgacac tttcttctgg atatttgtat tttgattgtg
    19141 ttctttgtgc ttttgtcatc agagagaaga ccacagtcaa tacaccaact cgtccaatgt
    19201 acataagcgc tataatgatc aatttcccta ttgcagtcaa gtctggcgtc aatcccatcg
    19261 taagacctac tgtaccaaac gcagagaaga cctcaaaagc aatatattcg atgccagaag
    19321 ttttaggtat cgtttcagtc accgataaaa tcatgatggc gactacacac aacgtcaacg
    19381 taacaaaaaa taaagtaagt gcccgcaaca ctgcgccttg tcggatggta cggccaaatg
    19441 cttccgctct cgtacgtcct ttcaacatcg aacgcatctg gatcagcaac acacctaacg
    19501 tagttgtttt caatccgccg gccgtcgacc cagatgttcc gccgatgtac atcaaaaaca
    19561 tcgttaaaat cagacctgca tggctcatat ggaggtaatc aactgaataa tatccagccg
    19621 ttctaggtgt cacactcata aaaaaggtgt ttgccaatcg ttgaaacata ttcccttcca
    19681 ctagcgttgc cccattgcgc tctgtgaaaa agaacacaac gaatccgcct aataaaaggg
    19741 tacctgtaac ggttaatgcg atttttgaat gaatcgtcat cttctttttc tgatgatatt
    19801 ccagtaaatc acgccatacg atgaagccta acccaccaga aatgatcaaa gcagatacca
    19861 ccatgatcaa ataaacattt tgctggtgat tggctaaact gtctcctaac aaatcaaacc
    19921 ctgcattaca aaaactagag attgcatgga acaaactgta ccagatacct ttcaaccagc
    19981 caaattcagg cacaaataca aaagataaag caaccgtacc aatcaattgg atcacaaagg
    20041 ccaattttag gatgtaaagc atgagcttca tcacacctga tacttcttct agattcagcg
    20101 cttcttttaa tataatacgt gtgctgagac tgactttctt ctttatcaat gcaaagcata
    20161 gaatcggaac catcataaaa ccaagaccgc cgatctcaat caaaagcatg atcaaaaatt
    20221 gaccagcact gttccaatgc tctgctgtat tcaatgttgt caatcccgtt acacagacag
    20281 cagaagttgc agtgaaaagc gcatcgataa aatttgtccc atgcccacta ttactaaaaa
    20341 atggtagggt aagtaggccg ccgccaacta aaatcaggag aaaaaatcct aatgcgagaa
    20401 gctgaacagc cggcaaacgt ttctttattt gtttgctttg tttaatcatc gttttcctct
    20461 tttaaaaaaa gataccactt agtcagtggt acccatattt tttactttga tcaaccttgt
    20521 tacttctgcg cgctcgtttt cttccagctt catacgaata taatagatcg tttcttctaa
    20581 ttgagggatc gtcatatatt ctaatgcatt gactcgccga cgtgtctttt cgatttcact
    20641 cgccatcagt tgacaagttt tttcgatttc tgtcagttcc aaaagttttg gaagcagggt
    20701 agtaaaccga tcgattgacc gatccagttc ggcactggaa ttcaaatacc cgtattccaa
    20761 aggtgtgtct gtcagctgtt catcatattc aaaattcatc acaggtactt ttacactcat
    20821 gatatttttg tctacgatag acaaatcaac gttttccgcc ggcaaaacaa ataattcttc
    20881 gatgaaaggt tccgtgatcg aagcatttgc caatacaaaa tcagacattg cttctgtcat
    20941 agactcttcc atttcttgac gcagctggtt gttctttcga atcaacagga tgaattgacg
    21001 catcagttca tcttgcttat cttttaataa tttatgccct cttgtggcag ttgtcaactg
    21061 ttttttcaat cgagtcaatt ccatgcgggt cggattcaca ttcaatcgtg ccatcagaac
    21121 tcacttccct tctggcagat atttgtccag catgtcgtct ttgattcgtt ttaattcggt
    21181 tcttggcaac atcgatagca gttcccaccc tagatcaagt gtttcagtaa tcgaacggtt
    21241 cgtgtagaat ccttgattga catattcttt ttcaaaccgc tcagcaaact tcgcatagat
    21301 cttgtctaca tcagataaag cagattcacc taaaactacg gctaattctt ttgcctgttt
    21361 tccttgtgca taggctgcaa aaagctgatt catcgtagct gcatggtctt ctcttgtctt
    21421 tccgggtcca gttcccttat cttttaaccg cgaaagagac gggagtacat caattggtgg
    21481 ctgaatccca ctcttgtaaa gctctctagt caagatgatc tgtccttctg taatataacc
    21541 agtcaagtca gggattggat gagttttatc gtcttctggc attgttagaa tcggaatttg
    21601 agtaaccgat ccttttagtc cgcggattct tcctgctcgt tcgaataaag tagctaagtt
    21661 cgtgtaaaga tacccaggat atcctcttct tcctggaact tctcgccgtg ctgcagaaat
    21721 ctctcgcaat gcctctgcat agttggtcat gtctgtcatg ataaccaaaa catgcattcc
    21781 cttttcataa gcaagatatt ctgcagcagt caaagccata cgaggagtag cgatacgttc
    21841 gattgcaggg tcgtttgcta aattcataaa catgacagaa cggtcgattg cgcccgtttg
    21901 acgaaaatct tccatgaaaa attcagcttc ttcaaaagtg attccaatag cagcaaagac
    21961 tacggcgaaa tcatctgatg aatcgagtac cgttgcttgt cgcgcgatct gagcagctaa
    22021 ttctttatga ggaagaccag agccagaaaa tactggtaat ttttgcccac gaactaatgt
    22081 attcaaatga tcgattgcag agatacctgt ttggataaac tcatctggat aatcccgagc
    22141 tactggattg attacttctc cgttaatatc caaatgcttt tcaggcagga tctctggtcc
    22201 gttatctttc ggtcgtccca aaccatcgaa tactcgaccg atcatgtctt ccgatacacc
    22261 tagttctaaa ggatggccta aaaatcgtac agaagagtca cgtaaattga taccacttgt
    22321 tccttcgaat atttggacca ttgctttatc ttcttggact tccaatactt gtccacgacg
    22381 aatctcgcca ttttgcatac ggacttcgat taattcttca tatttgacac cagcaacttt
    22441 ttcaaccgcc atcaatggac cgacgacttc accgatcgta cgatattctt taatcattgc
    22501 tcatgcctcc ttctgaaacg atcgtttgca tcgtttgctt gatttcacca ataatttgat
    22561 ctagtttttc taattctgtt tccgggatat atttgcttcg tccgatcctt tcacgaatcg
    22621 atacggtacc gtccatgatc tcacttaaat aagcacctaa cgacaacgca cggcgtcctt
    22681 cttcaccaaa ggtcaagatc atcttcaaca ttttgaattg tttctctctt gaagtgaatg
    22741 tatcgacatc atcaaaagca ttttgctgca aataatcttc acgaatggat tttgccactt
    22801 ctaatgtcag acggtcatta tctgaaaggg aatcaatccc taccaaacgc acgatttcat
    22861 tcagctgctg ttcttcctgc aaaatacgca tgccctcggt gaccatggat gcccagtcag
    22921 cttgaaggat atgatccata tacttcccta cttctgttga ataaagagaa tagctttgta
    22981 tccaatcgat cgaaggaaaa tgtctttttt gggctaaact ggaatcaaga ccccagaata
    23041 cttttacgac ccgcaatgta ttttgtgtga ctggttccga aatatcccct ccagacgggg
    23101 atacagcact gattgcggtt atacttcctt cgcgaccttc tgatcctaat gcaatgactc
    23161 ttccagatcg ttcatagtat tccgcaagcc gtgaaccaag ataagctgga taaccttcat
    23221 caccaggcat ttcttccaaa cgtccgctca tctcacgaag tgcttctgcc catcgtgaag
    23281 tggagtctgc catgatagca acgtcataac ccatgtcacg gaaatattcc gcaattgtga
    23341 ttcccgtata gatagaagct tctcgagctg ctactggcat gtttgatgta ttagcaatca
    23401 ataccgtacg ttccatcaac gattctcctg tattagggtc aatcaattca gggaattcat
    23461 ttaaaacatc cgtcatctca tttccacgct cgccacatcc cacatagaca accatgtcca
    23521 catcggccca ttttgcaatt tgatgctgaa cgactgtttt ccctgcacca aaaggaccag
    23581 gtacagctgc tgctcctcct tttgtcactg ggaagaatgt atcaatcact cgctgtccag
    23641 tgatcatggg tgcatccggg tttaattttt ctttgattgg ccgactacgt cttactggcc
    23701 atttttgaag catcgtcaaa tttttgtcac cattttcggt ttcaatgaca catactgttt
    23761 catcaatagt gaatgaacct ttttcgattg ttttgatcgt tccttttatc ccatttggca
    23821 ccatgatttt atgttttata acttttgttt cttctacgat cccgataaca tctccagcac
    23881 tcaccgtttc tccggcttct actgttggtt cgaaccacca ttttttctca tggtccaatg
    23941 cgggcaattg aactccgcgc cctaagaaat tactttgtgt gatttccata aaggtatcta
    24001 atggccgttg gattccgtca aacatttgtg aaatgatccc tggtcctaat tcaacagata
    24061 aagcttcacc agtagagcga accggttcgc ctggaccaat tccagaagtc tcttcatata
    24121 cttgaataga cgccacatcg ccacgcatct cgataatttc tccgataact cctagatctc
    24181 ccactagaca catgtcttgg atactggcat cagacatgtt ctcagccatt acaagaggtc
    24241 cggagacttt tatgattttt ccaatttgca aaatttctat ctccttttca tactataaga
    24301 tattttgacc tactgctttt tctacgtttt tttgaatttc ttccagccct atccctaatg
    24361 atccttgatg attgggaatc aatacgatag caggtatcat ttgttccttg taacgttcga
    24421 tcgtttctgg gctcaatgct gcacattgct ctgtgacata aatcacgcca tactcattct
    24481 ttgccatttc ttcaattgcc tgacgaacgg tttgcttggt tgtaccataa cgaacatcaa
    24541 agccaaacaa tttgaatggc aaaacagaat ctttgtcgcc aactacaccg atcttatgcg
    24601 tcatagactt gtctcacccg ctttctgatt gtttctgatt cgaaatggct tcttttgccg
    24661 ataaccagta aacgaaggtt tttgctttct acctcttttg cgttcaaaaa ggctagtagt
    24721 ggcagtggtc cgaaagcctg ggtttgtgct tcttgataca atgaacttag ataatcatct
    24781 ttcaactgtt caagctttac gagatcaata tttccctcac ggatgacagg gcgcagaaaa
    24841 tcagcataat ctgtctctaa tagaaaattc gtgtaatctt ccatatcttt atctacaaag
    24901 ttcaaaaata cttctttttc gatacttccc gagctggaaa gtaccgtcgt catgaaccca
    24961 tatgttcttt tctgcaatat tcctcgagcc atcgttgtga tattggtcaa gtcaataaaa
    25021 gcaatgactt ctgtcagcaa ttcttgatat cctagcttat cggcaagccg tcgctgctct
    25081 gtcaaaaact gtcgatcata aatcacgtca attccctgaa gaatggtaga ctcttcaaaa
    25141 taatcacgta cttctcgtat actgtccata atgctttcag gaagttcatt tgaaacttgc
    25201 gtatgaatgg catccttcaa ggtttctaat gaataaaatc catcgtcgat atacaaatgg
    25261 tccagatttt gatttgtcat ctcagctttt gtcaaaacct tcagattatg gaaagtataa
    25321 cgcatcgtat agatccaaac gatttcaggc tccggtgctg attcctttaa ccagcgaaat
    25381 agacggctct gctcctttaa aagattctgt tcgaagtcat attcatatcc gtcataaata
    25441 tatggactgt aaatcgtgga tttcaatatt tcaccaagtg cttcgacaga atttgtttga
    25501 atcatccgtt caaatgtctc attggacaaa agttcaagct ctcttccgcg aatcaaggga
    25561 tttagttcgt ggtattccat acactgatcc ctccttattc gaataattga ttggcaatct
    25621 caaaactcat cgttccttgg atatcttgta caaggctgct gaaaagaaaa ttatactgaa
    25681 ctccttgatc gtttatcaga aaacccgctt gatcagcaac agtttcatca cttaaaacca
    25741 tcataaaggg aagttcattg ttc
//
LOCUS       DACEZN010000021        24808 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEZN010000021 DACEZN010000000
VERSION     DACEZN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.021"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IXH60_000689"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IXH60_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1989653.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IXH60_000690"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IXH60_000690"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1989654.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IXH60_000691"
     CDS             complement(2169..2906)
                     /locus_tag="IXH60_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1989655.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IXH60_000692"
     CDS             complement(2997..3524)
                     /locus_tag="IXH60_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1989656.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IXH60_000693"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IXH60_000693"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1989657.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IXH60_000694"
     CDS             complement(4401..4886)
                     /locus_tag="IXH60_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1989658.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IXH60_000695"
     CDS             complement(5039..5236)
                     /locus_tag="IXH60_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989659.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IXH60_000696"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IXH60_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1989660.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IXH60_000697"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IXH60_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1989661.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IXH60_000698"
     CDS             9211..9423
                     /locus_tag="IXH60_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1989662.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IXH60_000699"
     CDS             9425..11104
                     /locus_tag="IXH60_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1989663.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11365..13044)
                     /locus_tag="IXH60_000700"
     CDS             complement(11365..13044)
                     /locus_tag="IXH60_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1989664.1"
                     /translation="MNTKQSDKRITALYARLSRDDEKDGISGSIQNQKVILEKYAKDN
                     HLPNPRFFYDDGFSGVTFTRPAFMEIMELAEQGMVGTIVVKDHSRLGRNRLVVGQLLE
                     EDFVRLDIRYIAIMDNIDTEKGISDIVPMQDLFNEWHAKNTSDKVRRVMQSKGMSGKP
                     LTTNPPFGYMKNPENKDEWIVDEPAAKVVKQIFEMCVSGLGPTQIAKQLHSEKVLTPT
                     EYWNSIGRKCSKPPAVPYNWCAATVADILDKQEYCGDTVNFRTTSKSFKLKKRLERPQ
                     EDWQIFENTHPAIIDRETFELVQELRKHRRRPNRTGEISMFSGLLYCADCGEKLYYSV
                     TNNYKREQAYFFCSAYRKNSNVCSAHYIREKIVEQLVLESMQRVLWYVQSYEKLFAQR
                     QLAEFGEKQKKELTEKRRELDKAKLRVREIDGIIQKLYEDNAAGKISDGRFATMSMSL
                     ENEQSELKDRIPSLEDELENAKIKTEGLQQFIDKAKQVTRLEALTPELVHEFIEKIVV
                     SAPKYKDGKRYQSVEIYYNGVGIIREPTSEEMEEYFEEHIKNKPSLKAKTA"
     gene            complement(13147..13251)
                     /locus_tag="IXH60_000701"
     CDS             complement(13147..13251)
                     /locus_tag="IXH60_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989665.1"
                     /translation="MEIGGTFYSVSSHFNRTANESLQEKTQRIILNKL"
     gene            complement(13416..14774)
                     /locus_tag="IXH60_000702"
     CDS             complement(13416..14774)
                     /locus_tag="IXH60_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1989666.1"
                     /translation="MATTSLWHIEGRLKDLIAYVENPEKTRADTPNLQPLWDVFSYVS
                     RPEATEQGEYVSAVNCLKEIALQQMILTKKQYGKENGYIAWHGYQSFKPGEVTPEQAH
                     KIGLQLAKEMWGKQYQIIVTTHLDKDHLHNHFCFNSVSFLDGRKYNYSKTEQKKLREL
                     SDRICREHGLSVIENPHKAPSRQVWLDEKSGKPTRYNVYREDVKEAINFSRRPYYMEE
                     YLRRKGYITDFTGRHWKIRLPQYEHFTRLDTLDKRWTPENIERTMGAYASFGNRRATI
                     NYPPQMPQDLKNWFQPFHKTSHIYKLYLHYCYLLGVLPKNTEYKPTSPYLKEDLRKLE
                     VFSEQVRYMSKYGIETFDDLYADREKLQGGMDKLIAYRTKLQNKIRRASPAEKETLRE
                     EKSKVTEQITALRKQLKLNKGIEERSVKIQEKTDMLYANEYRAKEEEQHRKSQRRERN
                     VR"
     gene            complement(14774..15067)
                     /locus_tag="IXH60_000703"
     CDS             complement(14774..15067)
                     /locus_tag="IXH60_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAQ1989667.1"
                     /translation="MNKENNQKVRIKVRLTEEENEKLKRCAEACGLSQSEFIRQLCIG
                     KAPKPKPTKEFWKLLNTLYSVHNSFQKCAKYEHSTLEICKEIECLILDLQEAG"
     gene            complement(15810..16235)
                     /locus_tag="IXH60_000704"
     CDS             complement(15810..16235)
                     /locus_tag="IXH60_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1989668.1"
                     /translation="MAYNHGKEERKWRIWKENEEKILREYGVDESTIEEIRTYDRAEF
                     NSNRRFYHWTNDVAEYLGEMADTEPQAEIYSVEDLLNEIDNEALYRALLTVDRRTLLI
                     LLLKMQGYTTKEIAPIVGLTMGAIYARLDYLRKKLKKIL"
     gene            complement(16679..17404)
                     /locus_tag="IXH60_000705"
     CDS             complement(16679..17404)
                     /locus_tag="IXH60_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HAQ1989669.1"
                     /translation="MKKKPKSLIKKLIFHSIAALFCITAIISLVFGVKGYSMYREAVK
                     AYPISQMVSSIQNRENFVEFDELPTIYIDAVISAEDKRFESHCGVDFIAIGRAVWNDI
                     KAMSFVEGGNTITQQIAKNQYYTQEKKLERKFAEIFTAIELEKYCSKQEIFELYVNTI
                     YFGDEYYGIYDAAKGYYGKLPSELSDYEAIMLAGLPNAPSAYSLNTNPDLAFSRMNIV
                     LSRMVECGVITQEQADDILVNGN"
     gene            complement(17438..17827)
                     /locus_tag="IXH60_000706"
     CDS             complement(17438..17827)
                     /locus_tag="IXH60_000706"
                     /inference="COORDINATES: protein motif:HMM:NF012610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1989670.1"
                     /translation="MNYTEWAFHDVEPVYLQLIQKIEYAILSRQLSTGEEIPSVREMA
                     KWLHISPNTVMKAYMRLNKSNLITSSRNEHYSVIDNEQYILQMRDEKVRQLCITYLSN
                     MINLGFSRKEATEFLVEYSNKLKEPNE"
     gene            complement(17983..18483)
                     /locus_tag="IXH60_000707"
     CDS             complement(17983..18483)
                     /locus_tag="IXH60_000707"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989671.1"
                     /translation="MKKLKSFVALSLALCMMLCFAGCSSNHDKGNLKNNLIDGFNSWL
                     ESFSKHAVTKDKNLQGERENDIDQYTGSYTASYDGFTGEEFIFGGTALERDNGSNLEA
                     IYSLTITSGKATLYWLSSEEEHIICKENADNTYQFTLSSGDNYIVLRGDNFSGNLELT
                     VEDVSN"
     gene            complement(18497..18781)
                     /locus_tag="IXH60_000708"
     CDS             complement(18497..18781)
                     /locus_tag="IXH60_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989672.1"
                     /translation="MKNKILTAISTIMLFVPWTILPLRTFDWALESPVAEIMISCYAA
                     FMIFSGIFTIVSYVKAKVQNNLMKVDLVVNSLYAVFGIGAFILMAITKFS"
     gene            complement(18789..19559)
                     /locus_tag="IXH60_000709"
     CDS             complement(18789..19559)
                     /locus_tag="IXH60_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1989673.1"
                     /translation="MSFLSLIGVEFMKIKRSKIFFILFAATVILWIPSILNADMNFSM
                     QAEGISPENNFFIQGFMGMAWFMFPASMVVGTVLLSQTERTNKGILKMLALPVSTVKL
                     CLAKFVVLLALAASQILMMIGMYYMSAAIVTGTQDYNFILSPLFVLKEAGFIAVSTIP
                     MLAFFWLLAVCIQTPIFSIGIGLASIVPSVLIINTKAWFAYPMSYTFFVITSEYGKLA
                     KNLTTAQVDFFPWIPVAIAVTIVCLCISCFRFGQAERR"
     gene            complement(19556..20278)
                     /locus_tag="IXH60_000710"
     CDS             complement(19556..20278)
                     /locus_tag="IXH60_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989674.1"
                     /translation="MSLSLECKKAKRTGFIPAFLCGGILAAAVPIVNMAVRSEIYLGQ
                     QSSPIQILMGANWQMMAMLNILLVVAGSCLLYHTEFADNAMQKMKSLPIRESSVFFGK
                     FVLTSIMSVVVLAIEAASISFCVSNWFEIGNDFWLELGKNFGYSFLLMLPCVLLSMLI
                     SSACKNMWVSLGIGVVCVFTATMLPTSNFVLSLFPFATPFQVFVNTELAQAMHYVYAA
                     MIEIVVIGIAELIFIKVRRSFE"
     gene            complement(20275..21030)
                     /locus_tag="IXH60_000711"
     CDS             complement(20275..21030)
                     /locus_tag="IXH60_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989675.1"
                     /translation="MNIIETKNLTKSYADFTAVSNVNLHIPKGTVYGFLGPNGAGKST
                     TMKMFLGLTKPTSGSFTIDGKKYPEARVEILKEVGSFIEAPAFYGNLSGEENLEIIRK
                     ILGLPKSAVSEALEIVGLTQFKKRLAKKYSLGMKQRLGLASALIGKPPILILDEPTNG
                     LDPVGIHEIRTLIRSLPEKFNCTVFVSSHLLSEVELMADTVGILNHGRLLFEGTLEQL
                     RSGAAAQGYPTDNLEDTFLALIDADNRQRGVTK"
     gene            21185..21889
                     /locus_tag="IXH60_000712"
     CDS             21185..21889
                     /locus_tag="IXH60_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1989676.1"
                     /translation="MDCEYLKNKRLLLVDDEQELLDMVSSIFADEGFCNLKTAKTVKE
                     ALTISETFQPELAILDVMLPDGNGFDLMKQLKSKSDYPVLFLTARGEDDDKFRGFGLG
                     ADDYIVKPFLPKELIYRVNAILRRSYKGENPIVKLSGSEIDFDRAEVIKNGQHIALTA
                     KEHDILAALYRNAGRIVTLDALCEAAWGDNPYGYENSLMAHIRRIREKIEINPSQPVS
                     LITIKGLGYKLIVEDK"
     gene            21891..23276
                     /locus_tag="IXH60_000713"
     CDS             21891..23276
                     /locus_tag="IXH60_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1989677.1"
                     /translation="MKSVPKLIKRFVGILMLSSLVILFMNFIIIAIIAATQAPNASPW
                     KTAEQAAESIEKSEQGYVMPDKMIEELNAQNVWAVYIDNATGECVWHSDNLPDTVPLE
                     YTASDIANTTRGYIDGYPTFTGEGEDGLIILGYPRDSFWKHMWPSWDYQFIANLPKTV
                     LIVLAINIFIVFLIYMAASTKLLKSIKPITDGIQALHTGQPVVLKEKGVLSEIAANIN
                     QTSEVLQSQNYQLKKKETARANWIAGVSHDIRTPLSMVMGYAGQLESSKQLPEEERQK
                     AAVIVKQSQRMKNLINDLNLASKLEYNMQPTHIEQVNVISAVRQVVVDFINLDISGKY
                     PIEWLTDSDLNTLIINADTDLIKRAVSNLIQNSINHNAEGCTIYVAVKECNRSSNIVV
                     SDNGVGATDEQIDKLNHSPHYMVCDENTSEQRHGLGLLLVKQIAAAHKGTVEIRHSEY
                     GGLEVEIDLPL"
     gene            complement(23368..23742)
                     /locus_tag="IXH60_000714"
     CDS             complement(23368..23742)
                     /locus_tag="IXH60_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HAQ1989678.1"
                     /translation="MAKSLFEEMGGRYKRQGDYLIPCITLSDEEEQSIGLWGERHLHY
                     LKEYRRGTYINLLTRGRLNTYLADIDRQAEELFFRLVEQMKQAQGITEHLKAENQLEW
                     VQRLSNIRACAREIVEKEIIYA"
     gene            complement(23920..24120)
                     /locus_tag="IXH60_000715"
     CDS             complement(23920..24120)
                     /locus_tag="IXH60_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1989679.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(24270..24431)
                     /locus_tag="IXH60_000716"
                     /pseudo
     CDS             complement(24270..24431)
                     /locus_tag="IXH60_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         7065 a         5388 c         4536 g         7819 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 ggggcaccct ccgtaaggaa ggtgccccaa ggcttttgtg ttttaagttt aataatagtt
    11341 gtgttaaatc ggtgtagcca atggctatgc cgtttttgcc ttcaaggaag gcttgttctt
    11401 gatatgctcc tcaaagtatt cttccatttc ttcggaagtg ggttcacgga tgattccaac
    11461 gccgttgtag taaatttcta cgctttggta tcgctttccg tccttgtatt taggtgcaga
    11521 tactacgatt ttctcaataa attcgtgtac caactcgggc gtgagagctt cgaggcgggt
    11581 aacctgcttc gctttgtcaa taaattgctg taagccttcg gttttgattt ttgcgttctc
    11641 aagctcatcc tccaaagagg gaatacggtc ttttaactcg ctctgctcgt tttccagtga
    11701 catcgacatt gtggcaaaac gcccgtcgct gattttaccc gctgcgttgt cctcataaag
    11761 tttttggata attccgtcaa tctcacggac acgcagtttc gccttatcaa gctccctgcg
    11821 cttttcagta agctctttct tttgcttctc gccaaactcc gccaactgcc tttgtgcgaa
    11881 taacttttcg taactctgca cataccataa taccctctgc atactctcaa gaacgagttg
    11941 ctccacaatc ttttcccgaa tatagtgggc agagcacaca ttagagtttt tgcggtaagc
    12001 ggaacaaaag aaatatgcct gttcccgttt gtagttgttc gttacgctgt aatacagttt
    12061 ttcaccgcaa tccgcacagt agagcagtcc tgaaaacata ctgatctcgc ctgtgcgatt
    12121 cggtctgcga cggtgctttc gcaactcctg taccaactca aaggtttccc ggtcaatgat
    12181 tgcagggtgc gtgttctcaa atatctgcca gtcctcctgc ggtctttcca aacgcttttt
    12241 tagcttgaag gatttgcttg tggtacggaa gttgacagta tcaccgcaat actcttgttt
    12301 atcaagaata tctgcaactg ttgccgcaca ccaattatat ggaacggcgg gcggtttact
    12361 gcattttctg ccgatactgt tccagtattc cgtaggcgta agcacttttt cagaatgaag
    12421 ctgttttgct atctgcgtcg gtcctaagcc tgaaacacac atctcaaaga tttgttttac
    12481 cacctttgcg gcaggctcgt ccacaatcca ttcgtctttg ttttcgggat ttttcatata
    12541 cccgaaaggc ggattggtag taagcggttt gcctgacatc cccttgcttt gcattaccct
    12601 gcgtacctta tcgctggtat tttttgcgtg ccattcgttg aataagtcct gcatcggaac
    12661 aatatcgctg ataccttttt cggtgtcaat gttgtccata atagcgatat atcgaatatc
    12721 caaacggaca aaatcttctt ccaaaagctg accgacaaca agacggtttc tgccaagccg
    12781 ggaatgatct ttgactacga tagtccctac cataccctgt tcggcaagtt ccataatttc
    12841 cataaatgca ggtcttgtaa aggttacccc tgaaaatcca tcgtcataga aaaaacgggg
    12901 attcggcaga tgattatcct ttgcgtactt ttcaagaatg actttttgat tttgaatact
    12961 gcctgatatg ccgtctttct cgtcatcacg ggaaagcctt gcgtacagag cggtaattcg
    13021 tttatctgac tgttttgtgt tcatagttct cctttccgca gccagatatg atttgatttg
    13081 taggtgaacc catcatacca tatccgccgc tgaaattcaa cgctttaatg tgatacagcg
    13141 aaagttttac agtttgttca ggataattcg ctgtgtcttt tcctgcaagc tttcgtttgc
    13201 agttctgtta aaatgactgg atacggaata aaatgtacct ccgatttcca tatcaaagtt
    13261 gatgggatag ggatacctgc ttttacgaaa ccactcaatg cgttctgctt ttgtcattga
    13321 cagaagcatt ttcgtagttt cttcaattgc ttcgtgaata tctctgtaat catcgcaagg
    13381 gtagccctgt tcatcaaggt aaagaatatt gtctgtcatc tcacgtttct ctcccttctc
    13441 tgtgattttc tgtgttgttc ttcctctttt gctctgtact cattggcata gagcatatct
    13501 gttttctctt ggattttcac ggagcgttcc tcaatgcctt tgtttagttt taattgtttt
    13561 cgtaaagcag taatctgttc tgtaacctta gacttttctt ctctaagcgt ttctttttcg
    13621 gctggtgagg ctctgcggat tttgttttgc agcttggtgc ggtaggcaat cagcttatcc
    13681 attccaccct gtaatttctc tctgtccgca tataaatcgt caaaggtttc aattccgtat
    13741 ttgctcatat atcgcacctg ttcggaaaac acctcaagtt ttcttaaatc ttctttgaga
    13801 tatgggcttg tcggtttgta ttccgtattt tttggaagaa cgcctaacag atagcagtag
    13861 tggagataca gtttgtagat atggcttgtc ttgtgaaaag gttgaaacca attctttaag
    13921 tcctgcggca tttggggcgg atagttaatt gtcgctctgc ggtttccaaa agaagcgtat
    13981 gctcccattg ttctctcaat attttcggga gtccatcttt tatccagcgt atcaagcctt
    14041 gtaaaatgct cgtactgcgg caatcgtatc ttccaatgcc tgcctgtaaa gtcggtaata
    14101 tatccttttc gcctgagata ttcctccata taataggggc ggcggctgaa attgattgct
    14161 tcttttacat cttcacggta gacattgtac cttgtgggtt ttccgctttt ctcgtccagc
    14221 catacctgcc ttgacggcgc tttatgcgga ttttcgataa ccgataagcc gtgctcccgg
    14281 caaatccggt cggataactc acggagtttt ttctgctctg ttttggaata gttgtatttc
    14341 ctgccgtcca aaaaggaaac cgaattgaag caaaaatgat tgtgcagatg atctttgtca
    14401 agatgggtgg taacgataat ctgatactgc tttccccaca tttcctttgc caactgcaaa
    14461 ccgattttat gtgcctgttc gggtgtgact tcgccgggtt taaaactttg ataaccgtgc
    14521 caagcgatat atccgttttc cttgccgtac tgctttttcg ttaaaatcat ctgttgtaat
    14581 gcaatttctt tcagacagtt gacagccgaa acatactcac cctgctcggt ggcttcgggg
    14641 cggctgacat aggaaaacac atcccacaac ggctgtaagt tcggagtatc ggctcttgtc
    14701 ttttcgggat tttctacata ggcaatgagg tcttttagtc tgccttcgat atgccataga
    14761 cttgttgttg ccattatcct gcctcctgca aatcaaggat caggcattca atttccttac
    14821 agatttctaa agtagaatgt tcatacttag cacatttctg aaagctgtta tggacagagt
    14881 aaagggtatt caatagtttc caaaactctt ttgtaggttt cggcttcggt gcttttccaa
    14941 tgcaaagctg gcggataaac tccgattgtg acagtccgca ggcttctgca cagcgtttta
    15001 gtttttcatt ttcttcttcg gttaggcgta ctttgatacg cactttttga ttgttttctt
    15061 tgttcaaaat tatcatctcc gttcttgtgt tttatctttt ataggggtat tcaggggtgt
    15121 tcccctgagt tagtccacac gcctttaggc tgtggatttg gattgtgggg gtcacaatcc
    15181 gatgctcgct atccctgtgg acacgtcgtc acaagctccg tatcgctcat ttccgtgcag
    15241 gcacgaaaaa tcgctcactt cactgctcct ccttttccca aaaagtctgg cgacttttcg
    15301 ggaaccctgt gtgacagggc tgcttttctg tgcagccgac ttcttccata cctgccttta
    15361 cccgccgaaa agaaaatcct atgtccctgc acctccgttc tggatatgat ttcttccttt
    15421 gatttcggac atacaaaaaa gccgccacac gacatcacga atggcagggg gattcccctc
    15481 ctgtccaata tcgcaatgct atgtaacggc tttgaaattc aaaacctttg ctgcatacac
    15541 caaccgagaa aagcagcata ctcctcggct gcggtcagcc ctttgccatc ggctgacttt
    15601 taagcagatt gctttatgtg attttcaatt ttcaagtaat tccctttcgg gtattttaga
    15661 ataccatagc ggagatatgg ctgtcaagat tttgcataaa cttttagtat accctgtgga
    15721 cagagatttt attcatctgc cttaaagatt gggtgagaga attttatccc tcacccaata
    15781 gcccatcaaa aatggattgt cttagaaatc tataaaattt tcttcaactt ctttcgcagg
    15841 tagtctaacc ttgcgtatat cgctcccatt gtcagcccga caatcggggc aatctccttt
    15901 gtggtatatc cctgcatttt cagcaggaga atgagcaggg tacgcctgtc cactgtgagc
    15961 aatgcccgat atagtgcttc gttgtcgatt tcatttagca aatcttcaac ggaatatatt
    16021 tctgcctgcg gctcggtatc tgccatttct cccagatact ccgcaacatc atttgtccaa
    16081 tggtaaaacc gcctgttgga attaaattct gctctgtcgt aagtacgaat ttcttcaatc
    16141 gtgctttcat caacgccgta ctcacgcaat atcttttctt cattctcttt ccagatacgc
    16201 cacttgcgtt cttcctttcc gtgattgtat gccatgtcct ttacctccag tctgaatttt
    16261 tgaaatataa aaatccagat aagagggcgg aggacgacag ccagtacccg aagcaggctt
    16321 atcctgctga cagacaaaaa gtgacaaaag aaaaaccgca aaggctgtca gacccttgcg
    16381 gctataagat attaaagttc tattatgtgg atatttggcg tctacttcga gggtataata
    16441 tccccatata agaacgagaa tttttttaac agattatctc tattcctatc tgacgctatg
    16501 taggtaattg ctgcttctaa ttagcagcgg tgtggcggct ttacaccaac acataaaaat
    16561 ccctccaaac tcgaaacggg tttagaggga tagtcgcttg agcgtgacaa tacagcctac
    16621 caaaacaccg ttttttttat ttggtaggct taccgcattt attgattgga aagtatggtt
    16681 aattaccgtt tactaaaatg tcatcagctt gttcttgcgt aattacgcca cattcgacca
    16741 ttcgtgataa aacgatattc atacggctaa atgctaagtc gggattagta ttcaacgaat
    16801 aggcagacgg ggcgttaggc aagcccgcta acattatggc ttcataatca cttaattcag
    16861 agggcaattt tccgtaataa ccttttgcag cgtcataaat tccgtaatat tcatcgccaa
    16921 aatagattgt gtttacatat aactcaaata tttcttgctt ggaacaatac ttttccagct
    16981 ctatagctgt aaatatctcc gcaaattttc tctcgagctt tttttcctgc gtatagtatt
    17041 gatttttagc tatttgctgt gtaattgtgt ttccaccttc tacaaaagac atagctttaa
    17101 tatcattcca aactgcacga ccaatggcga taaaatctac tccgcaatgg ctttcaaatc
    17161 ttttatcttc agccgaaatt actgcatcta tataaattgt cggtaattca tcaaattcga
    17221 caaagttttc tctattttgg attgatgata ccatttggga aatgggatat gcttttacag
    17281 cttctcgata catagagtat ccttttactc caaacacaag ggaaatgata gcggtaatgc
    17341 aaaacaaagc tgcaatagaa tgaaatatca atttttttat aaggcttttg ggtttctttt
    17401 tcatttgaca gttccttttg cactacattt aatggtttca ttcattcggc tctttcaatt
    17461 tgtttgaata ttcaaccaaa aactcagttg cttccttacg ggaaaatccg agatttatca
    17521 tattactcaa ataggtaata catagttgcc gtactttttc atctctcatt tgcaagatat
    17581 actgttcgtt gtcaattaca gaataatgtt cgtttctgga agaagtaatt aagttgcttt
    17641 tgttcagacg catatatgcc ttcattacgg tgttgggact aatatgtaac cattttgcca
    17701 tttcccgaac agagggtatt tcttctcccg tagaaagctg cctgcttaaa atcgcatact
    17761 caattttttg aatgagctgt aaatagacag gctcgacatc gtgaaatgcc cattcagtgt
    17821 aattcatcgt tttctccttt ttcacaccaa caaataatgt ggtctgtaat agaccaaatc
    17881 aaatagatta atgttgcaag tatgcctatg ggtatcagca gcaaacatat aaaagacaag
    17941 ctgataccca atattgcata ccgcaaaaat atccttattt ttttaattcg acacatcttc
    18001 aaccgttaat tcgagatttc cgctgaaatt atcacctctc agcacaatat agttatctcc
    18061 tgaactgagt gtaaattgat aagtgttatc tgcattttct ttgcaaatga tatgttcttc
    18121 ttccgaactc agccaataga gagtggcttt tccagatgta attgtcaacg aataaattgc
    18181 ttccagatta cttccgttgt ccctttctaa agctgtaccg ccgaaaataa actcctcgcc
    18241 agtaaatcca tcatacgaag ccgtgtaact acctgtatat tggtcaatgt cattttctct
    18301 ttcaccttga aggtttttat cttttgtaac ggcgtgtttg ctgaatgact ccaaccaact
    18361 attaaatccg tcaatcagat tgtttttcaa atttccttta tcgtgattgg agctacagcc
    18421 tgcaaaacaa agcatcatac aaagtgccaa agataaagca acaaatgatt ttaatttttt
    18481 cattttaatc gctcccttaa gaaaactttg taatagccat caaaatgaaa gcgccaatac
    18541 cgaatacggc ataaagacta ttcaccacca aatcaacttt catcaggttg ttctgcacct
    18601 tagctttcac ataagaaaca atagtaaaaa tcccactgaa aatcataaat gcggcatagc
    18661 aggaaatcat tatttctgca acaggcgact ccaatgccca atcgaaagtt cgcaaaggta
    18721 aaattgtcca cgggacaaaa agcataatgg tactgatagc ggttaagatt ttgtttttca
    18781 taatagcttt atctcctttc tgcctgccca aaacggaagc aggaaatgca caaacaaaca
    18841 atggtaacag cgattgctac aggtatccac ggaaaaaagt caacctgtgc agttgtaaga
    18901 tttttagcaa gttttccgta ttctgaagta ataacaaaaa acgtatacga cattgggtat
    18961 gcaaaccacg cttttgtgtt aattatcagg actgacggca caatggaagc taatccaata
    19021 ccgatggaaa aaatcggcgt ttgaatacat actgcaagca accagaaaaa cgccaacatt
    19081 ggtatcgttg aaacggctat aaaaccagcc tctttcagaa cgaatagcgg ggacaaaata
    19141 aaattgtaat cctgcgtccc tgttactatg gcagcactca tgtagtacat tccaatcatc
    19201 attaaaatct gcgatgcggc taaagcaagc aatacgacaa actttgccag acaaagtttt
    19261 actgtactga caggtaaagc aagcattttt aagatacctt tatttgttct ttcggtttgg
    19321 ctcaaaagaa cagtacccac caccatactt gcagggaaca taaaccacgc cattcccata
    19381 aaaccttgaa tgaaaaaatt gttttctgga gaaattcctt ctgcctgcat actgaaattc
    19441 atatcggcat tgagtatcga tggtatccag agaataaccg tcgcagcaaa caatataaaa
    19501 aatatctttg agcgttttat tttcataaac tcaacgccga tgagagataa aaaactcatt
    19561 caaacgacct cctaaccttt ataaatataa gctccgcaat accgataaca acgatttcaa
    19621 tcatcgccgc ataaacataa tgcatcgcct gtgccagttc cgtattcaca aagacttgaa
    19681 atggagtggc aaaaggaaaa agtgaaagaa caaagttgct tgtcggcagc attgtcgcag
    19741 taaacacgca cacaacgcca attcccagag atacccacat atttttacaa gctgatgaaa
    19801 tcaacatcga taacagcaca cacggtaaca tcagtaaaaa tgaatatccg aagttcttac
    19861 ctaattccaa ccaaaaatca tttccaattt caaaccagtt tgaaacacaa aacgaaatcg
    19921 aagcagcttc aatggcaagt acaactacgc tcataatcga ggtcaaaaca aatttaccaa
    19981 agaaaacgga gctttctctg ataggaagcg acttcatttt ttgcattgcg ttatcggcaa
    20041 actctgtatg atacaacagg cagcttcccg caacaacaag caggatatta agcatcgcca
    20101 tcatttgcca atttgctccc attagaattt gtattgggga gctttgctgt ccgagatata
    20161 tttcggaacg gactgccata ttgacaatcg gtacagccgc cgccaaaatg ccgccgcata
    20221 aaaaagcggg gataaatcct gttcgctttg cttttttaca ttccagagat aggctcattt
    20281 tgttactcct ctctggcggt tatctgcatc aatcaaggca aggaatgtat cttccaagtt
    20341 atcggtagga tacccttgtg cggcggcgcc gcttctgagc tgctcaagtg ttccttcaaa
    20401 cagcaaacgc ccgtgattta agatgccgac tgtatccgcc atcagctcaa cttctgacag
    20461 tagatgcgat gatacaaaca ccgtacaatt aaatttttca ggcagagaac gaataagtgt
    20521 tcttatctcg tgaatgccca cagggtcaag tccattggtc ggctcgtcca aaatcaaaat
    20581 cggcggcttt ccgattaaag cacttgcaag ccctaatctc tgtttcattc caagagaata
    20641 ctttttcgca agacgcttct tgaactgcgt aagccccaca atttcaagtg cttcggaaac
    20701 ggcagacttt ggcagtccca atatcttacg gataatttcc aagttttctt caccgcttaa
    20761 attcccgtaa aaagcagggg cttcaataaa tgagccaact tcttttaaaa tttccactct
    20821 cgcttcagga tacttttttc catcaatggt aaatgagccg cttgtaggtt ttgtaagacc
    20881 caaaaacatt ttcattgtag tggattttcc tgcaccgttc ggaccgagaa agccatatac
    20941 agttcctttt ggaatatgca gattgacatt cgatacggct gtaaaatcag cataggattt
    21001 tgtcaagttt ttagtttcaa tgatattcat agtgttttgc tcctttctca tcattgcaag
    21061 cttattctaa cttgccaacc ttgtgataac cttctcccta ccttacattt accttacatt
    21121 tctccgcaca gtagaaaaag gcggcgattt atgctataat ggcaatctga aaggagcata
    21181 gtatatggac tgtgaatact taaaaaacaa acgcctattg ctcgtcgatg atgagcaaga
    21241 gcttttagat atggtttctt ctatctttgc agacgaagga ttttgcaatc tcaaaacggc
    21301 aaaaacggtt aaagaagcac ttactatatc ggaaacattt caacctgagc ttgcaattct
    21361 tgatgtaatg ctgcctgatg gtaatggctt tgaccttatg aagcaattaa aaagcaagtc
    21421 tgattatcct gttttatttt tgacagctcg tggagaggac gatgataaat tccgtggatt
    21481 tgggcttgga gctgatgatt atattgtgaa gccgttcctg ccaaaagagc taatttaccg
    21541 tgtaaacgcc attctccgca gaagttataa gggagaaaat ccgattgtta aattgtcggg
    21601 tagtgagata gattttgacc gtgcggaagt cataaaaaat ggacagcata ttgctctcac
    21661 ggcaaaagag cacgatatac tcgccgcatt atatcgaaat gcaggtcgca ttgttacgct
    21721 tgacgcttta tgtgaagctg cttggggtga caatccgtat ggatatgaaa actctttaat
    21781 ggcgcatatt cgccgcattc gtgaaaagat tgaaatcaat ccttcgcagc ctgtttctct
    21841 aataacgatt aagggattag gatataagct catcgtggag gacaaataat atgaaaagcg
    21901 taccaaagtt aattaaacgc tttgtgggca ttttgatgtt aagctctctt gtaattcttt
    21961 ttatgaattt tattattata gcaattatag ctgccacaca agcaccgaat gcctctccgt
    22021 ggaaaacagc ggagcaagcc gcagaaagta tagaaaaatc agaacaaggc tatgtaatgc
    22081 ccgataaaat gattgaggag ctgaacgccc agaatgtgtg ggctgtttac attgataacg
    22141 caacgggaga atgtgtctgg cattcggata atcttcccga taccgtccct ctggaatata
    22201 cagcttccga tattgccaat accacccgtg gatatattga cggataccca acctttacag
    22261 gcgaaggcga agatgggctg attattcttg ggtacccaag agatagcttc tggaaacata
    22321 tgtggccgag ttgggactat caatttattg ccaacttgcc caaaaccgtc cttatcgttt
    22381 tagctatcaa tatttttatt gtttttctca tttatatggc ggcaagcaca aaactgctga
    22441 aatctataaa gcctattacg gacggcattc aagcattgca cacagggcaa cctgttgtgc
    22501 ttaaggaaaa gggtgtactc tcagaaatag ctgcaaacat caatcaaact tctgaagtgc
    22561 ttcaaagcca gaattatcag cttaagaaaa aagaaacagc aagggcaaat tggattgcag
    22621 gcgtatccca cgacatacgc acacctcttt caatggttat gggatatgca ggtcagttgg
    22681 aaagcagcaa acaattacca gaagaggaac ggcaaaaagc tgcagttatc gttaaacaaa
    22741 gtcaacgaat gaaaaatctt ataaatgacc tgaaccttgc ttcaaagttg gaatataata
    22801 tgcaaccaac acatattgaa caggtgaatg ttatttctgc cgttcggcaa gtagtggtag
    22861 atttcatcaa cttggatatt agcggtaaat atcctattga atggcttact gacagcgact
    22921 taaataccct tatcataaat gcagatacag acttgataaa aagagctgtc agtaatttga
    22981 tacaaaacag cataaaccat aatgcagagg gctgtacaat ttatgtcgct gttaaagagt
    23041 gtaacagaag cagtaatatt gttgtttcag ataatggtgt tggagctaca gatgagcaaa
    23101 tcgacaaatt aaatcattca ccgcactata tggtttgtga tgaaaataca tcagagcaac
    23161 gccacggctt aggtttgctg cttgtgaaac aaatcgctgc tgcccacaaa ggaacagtag
    23221 aaataagaca tagcgaatat ggtggacttg aagtcgaaat agatttaccc ttatagtcga
    23281 tttaatttca atacaaaata taaagagccg atgactgtga ttactcacaa gttcatcggc
    23341 tctttatatt ttatactatc tgtctgttta tgcgtaaata atctcttttt ccacaatctc
    23401 cctcgcacac gcccgtatat tactgagcct ttgtacccat tcaagctgat tttccgcctt
    23461 tagatgctcc gtgataccct gtgcctgttt catctgctct accagccgaa agaacagttc
    23521 ctccgcctgc ctgtcgatgt cggcaaggta tgtattcagc ctgccccttg tcagtagatt
    23581 gatatatgta cctctgcggt attctttgag atagtgtaag tgacgctctc cccataagcc
    23641 tatcgactgt tcttcctcgt cagataaggt aatgcacggg attaggtaat cgccctgtct
    23701 tttgtatctg ccgcccattt cctcaaataa tgattttgcc attttgcaat tcctccgtat
    23761 gatttgaatt tgcctacaat ctaatcattc tggctgatta caaggcgaaa gggaaacaac
    23821 ttccttacga agtgtctccc ttgtggcagg tatttttgtt actcgctttg ttctctcgta
    23881 aaaattgatg tctaccatca atttgattta cagataaaat taagctttta caacgtttgc
    23941 agcttgtggg ccgcggtctg attcttcaac gtcgaaagtc actgcttgac cttcttctaa
    24001 tgttttgaat ccgtcacctt ggatagctga gaagtgaacg aatacgtcgc ttccatcttc
    24061 acgtgagata aatccaaatc ctttttcagc gttgaaccat tttactgttc cgttttccat
    24121 aataatatcc tcctcgtgcc aaaagcacta atttaaattg taacattgct cgtgtacgat
    24181 agaggtgcaa cagatgtttc aacttctatt cccaaacaaa attacaatat aattgtacac
    24241 ttgcttcatt ttttttgcaa gaaagcaact tacttctctt ccatcattcc ttcttctctc
    24301 aaactaaaat atcgtttgcg tccgatgatc aagtggtcca gtacagtgat ccccatcatt
    24361 tctccacact tttgtatcct tttggtaaaa ttcatatcct gctgcgatgg aataacatta
    24421 ccagaaggat aatataggtt agtacacgta taaaaatatc ttttatattg acacaattta
    24481 tttaacaagt ataataataa ttgaaagtga atttaaacaa atatacacca taaacacaac
    24541 gtacctgcga atacgttgtg tttttttctt tttcaaaaaa agacgcttac cgctgcgaaa
    24601 cagtaagcgc cattgtcggc tctaatgttt atctagccaa tgactaaata atgccaaagt
    24661 taaaccaatt aacaatggca agagcacgtg ctccaaagtg aattcaaaca aatcatacac
    24721 ctcctcccac gaaaatctct gtggaagcga cgagccgaca ttttattata ccattattga
    24781 attttatttt ttatcaaata gattaaat
//
LOCUS       DACEZN010000022        24385 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEZN010000022 DACEZN010000000
VERSION     DACEZN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.022"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            105..686
                     /locus_tag="IXH60_000717"
                     /pseudo
     CDS             105..686
                     /locus_tag="IXH60_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            690..1331
                     /locus_tag="IXH60_000718"
     CDS             690..1331
                     /locus_tag="IXH60_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989680.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1328..2245
                     /locus_tag="IXH60_000719"
     CDS             1328..2245
                     /locus_tag="IXH60_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1989681.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2242..2913
                     /locus_tag="IXH60_000720"
     CDS             2242..2913
                     /locus_tag="IXH60_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989682.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3454..3942)
                     /locus_tag="IXH60_000721"
     CDS             complement(3454..3942)
                     /locus_tag="IXH60_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1989683.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4143..4409)
                     /locus_tag="IXH60_000722"
     CDS             complement(4143..4409)
                     /locus_tag="IXH60_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1989684.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4409..4672)
                     /locus_tag="IXH60_000723"
     CDS             complement(4409..4672)
                     /locus_tag="IXH60_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1989685.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4665..4937)
                     /locus_tag="IXH60_000724"
     CDS             complement(4665..4937)
                     /locus_tag="IXH60_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989686.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5148..5333
                     /locus_tag="IXH60_000725"
     CDS             5148..5333
                     /locus_tag="IXH60_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1989687.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5475..7298)
                     /locus_tag="IXH60_000726"
     CDS             complement(5475..7298)
                     /locus_tag="IXH60_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1989688.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7896..10601)
                     /locus_tag="IXH60_000727"
     CDS             complement(7896..10601)
                     /locus_tag="IXH60_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1989689.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10907..11854)
                     /locus_tag="IXH60_000728"
     CDS             complement(10907..11854)
                     /locus_tag="IXH60_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1989690.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            12044..12313
                     /gene="rpsN"
                     /locus_tag="IXH60_000729"
     CDS             12044..12313
                     /gene="rpsN"
                     /locus_tag="IXH60_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1989691.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12489..12851)
                     /locus_tag="IXH60_000730"
     CDS             complement(12489..12851)
                     /locus_tag="IXH60_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1989692.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12862..13983)
                     /locus_tag="IXH60_000731"
     CDS             complement(12862..13983)
                     /locus_tag="IXH60_000731"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1989693.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13999..14349)
                     /locus_tag="IXH60_000732"
     CDS             complement(13999..14349)
                     /locus_tag="IXH60_000732"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1989694.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14479..15990)
                     /locus_tag="IXH60_000733"
     CDS             complement(14479..15990)
                     /locus_tag="IXH60_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1989695.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16312..17664)
                     /locus_tag="IXH60_000734"
     CDS             complement(16312..17664)
                     /locus_tag="IXH60_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1989696.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17817..18893)
                     /locus_tag="IXH60_000735"
     CDS             complement(17817..18893)
                     /locus_tag="IXH60_000735"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1989697.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            19089..20834
                     /locus_tag="IXH60_000736"
     CDS             19089..20834
                     /locus_tag="IXH60_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1989698.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21114..21551)
                     /locus_tag="IXH60_000737"
     CDS             complement(21114..21551)
                     /locus_tag="IXH60_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1989699.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21535..22908)
                     /locus_tag="IXH60_000738"
     CDS             complement(21535..22908)
                     /locus_tag="IXH60_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989700.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23230..23718)
                     /locus_tag="IXH60_000739"
     CDS             complement(23230..23718)
                     /locus_tag="IXH60_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1989701.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23740..24342)
                     /locus_tag="IXH60_000740"
     CDS             complement(23740..24342)
                     /locus_tag="IXH60_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1989702.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6990 a         5195 c         4510 g         7690 t
ORIGIN      
        1 aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc ataccgaacg
       61 gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt aaactagcaa
      121 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatattt
      181 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
      241 ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc acgattacac
      301 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
      361 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
      421 aacgaggaaa agtatccagt gtcggcgaca ttttgaataa acatgttttt tacgtaaaag
      481 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcgggctg aaatacgtgc
      541 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
      601 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagcta
      661 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
      721 aaattctctc aaaaggtttt gaacatcttt atatttcagg acttgctttg atcattggta
      781 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
      841 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
      901 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
      961 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
     1021 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
     1081 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
     1141 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
     1201 attaccaacc agatctcatt ttggcaggta cgatccctgt aacaatttta gcactattgg
     1261 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
     1321 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
     1381 gagttgttcc ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
     1441 tggaatcact tcagaagcac agatcttagc aagtctagtt gccggaatgg ttgaacatta
     1501 tacagataaa aataccgcaa tcatcaacaa tctggctact acaactatca accatcaagc
     1561 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
     1621 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
     1681 atttgagaaa agatacggtc aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt
     1741 ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca gcgatctgaa
     1801 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
     1861 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
     1921 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
     1981 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
     2041 cccaccatat gatgctgctc cagttgtaga caacaagata ttagctgata cgcctcattt
     2101 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
     2161 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
     2221 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
     2281 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
     2341 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
     2401 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
     2461 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
     2521 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
     2581 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
     2641 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
     2701 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
     2761 ggagatatca ttatccgagg aaccaatgca acagagggca catcgattat tttagcaggt
     2821 gcattgccaa ctgcattaat ggcgatcatc acggactggc ttttaggtat tttggaaaga
     2881 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
     2941 cgaccatttg ttggtcgcaa aggctttttt agttctgtca tttttaaggc aataatccac
     3001 tctgattctt ccctccttat gggcaaagag ttgcttttct tagctattta tccattccgt
     3061 atactccaac ttcatactgc caacgggttc atatctgcat ccaccgacta agtttttttc
     3121 ttatccgcta aggcattttg ccaatccatt ttcccatcac tcgtcatgga attcatacca
     3181 catgatctct aaatactcgt ctgcttgttc tttgaattgg gcatcaattc ttacatcctt
     3241 tggcgtggct ttcatttaag ccttttccat cagtcctcga ttgttcgatg ccttcagctg
     3301 catcatcaaa actggtgact gccgtatcgg taacttcgat catcttttat acaatcggga
     3361 acttgccatc ctcatccttg gctaaaaaca tatctagcca ttccagcttc tcgccatggg
     3421 aggtttgcgg ttcctaaaac cggtacacga ttcttatgga ctgcatccgt cgcaactaga
     3481 cttggcgata cgatgatcct tcaccagaag agcacccccg taaaccaatt gatcgatata
     3541 ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg cattgccgtt
     3601 gatactgcta ttcatggccg aaagcgccac gactttaact tcttccgttt gagtgacatt
     3661 catgttcggc aatgtcgctt tggatacccg ctctgctaaa ggaacgacac tggtattgta
     3721 tttcgtatct gtatcttttc caaaagacca tgccaacgaa tcctctggga accaataaga
     3781 cgactccggt tgattgtcca ttgctttttt caacatactt ttggctgttt ctcgataaac
     3841 gacatttcca attgtcttcg cctcactttg gcgataatcg atggtcgttg ccactgccaa
     3901 cactaacaac gatgctccaa ttttactgat tttccttgtc acacttcttc ctccttgttt
     3961 tacataaacc cgtcaacatt ccattcctaa actcaaacaa tgacccttca aaggtggaga
     4021 tataggaaca aattaaagaa atcatgcaat aaatccaaga aggttggttt tagaacaaaa
     4081 aaagagtctc ctttttgggg agactctttg ttaactatct aaaaaggttg gctcttctca
     4141 cttcactcat aatgtgtcca ggcagaataa atttctacaa ctttattttc ctcatcaact
     4201 ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt atctgccttc atgtgtaggt
     4261 ctcagctttt caaaagactg cgtgggcata tatggatctt ccttcaatgt ctgaatcact
     4321 tcttcaaatt gtttctttaa attcgtttgc ttgatttttc ttaaatccac tttcgcagag
     4381 tttttgattg cgactgtata gttactcatc aaagagcttc ccaatcaata tcatcgatat
     4441 tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt atctaaagta cctgtttgct
     4501 caagaaaaag ggtttcttga atcgctctcc agtcttccag cccaatcaat acagcattgt
     4561 ttccactacg atcactaata atctcgattt cagtatgatt ttcatttacc tctttcaata
     4621 actgataaaa atttttacga gcagtgctag gattcatcgt tgtattagcc attgtctctc
     4681 ctcctttttc tgatataatt atagcgtact tgattaagta cgtcaaaaaa atacaataaa
     4741 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
     4801 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
     4861 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
     4921 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
     4981 acctgaggat acgcacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
     5041 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gtcattcatg ttcactatcg
     5101 atttgatact tgcttagacc gttgggtttg tgaatcacca attaaaaatg gcactgagtt
     5161 atggtaacaa aaaatggtca cttgacgagg ctgagattcc tacttatgaa gacacatggc
     5221 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
     5281 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
     5341 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
     5401 tgagaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
     5461 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
     5521 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
     5581 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
     5641 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
     5701 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
     5761 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
     5821 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
     5881 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
     5941 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
     6001 aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg caatgattgc atatgagatg
     6061 aacgcacccg taatattggc aatcgatttg cttaaccatc tttttccttc ttttttccat
     6121 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
     6181 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
     6241 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
     6301 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
     6361 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
     6421 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
     6481 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
     6541 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
     6601 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
     6661 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
     6721 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
     6781 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
     6841 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
     6901 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
     6961 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
     7021 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
     7081 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
     7141 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
     7201 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
     7261 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
     7321 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
     7381 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
     7441 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
     7501 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
     7561 atcttaactt ttggaagaga gatgtaaggc tgctttctac ccatcattct tactactcga
     7621 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
     7681 aataagctca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
     7741 tgatgcagag cagctttttt acaacctctt ggtacacggt gttcaatcag ctgaccctcg
     7801 gtattagctc aaaaaaacgg caaaatatga ggaactattt ttccattttt ttctctaata
     7861 ctcaggtcaa aacactgatt tcacaacctc tttttttaat gaaaaatatg gctctcatgc
     7921 gccgcttctt tgaatttcgt cattttttct tcgatgaacg gatagattgc cgttcctacg
     7981 attccaaaga caacaaacaa accgatcatg atccagattg ctttcgtagc atttggccaa
     8041 taaattcctc ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg caagaacgga
     8101 ttgatcattt ggaagaattt ccctgagact tggatcggat agttcccgcc tccgccagag
     8161 atcgacagta ctaagatgat aatagcgatc cctttaccga cattatcgaa taatgcagcc
     8221 aagacataga cgatcatcat gaacgctaag gcgatcaaga cagcaaatga cacgctgtag
     8281 accggatttt ggacatctac tcctagtaag aaaatattcc ctaatgtaac gatcaagctt
     8341 tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta
     8401 aaattgtttc ggtctttctt tcctaaatag tattctgtcg tagcaacact tgaaagaaga
     8461 acagcgccga cccacagaca tagtgcagta taaaacgggg tgctagctga accgttattt
     8521 gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct
     8581 tttgttgcgt ctagtttcaa taatttgatg acttcgccta gatcgacttc ttgttcgcct
     8641 tttcggatcg cgtctgctgc tttttggata ccagctttga tatttggcca gtcattgttg
     8701 atcaagtccg cagcagcgtt cacagctgtt tcgacttctg gcattttttc atttactgtt
     8761 gtcaatgtac cggtgatttc attttttagt gctggccaat cattttggat aaaatcagcg
     8821 gccaatccta atttttgctg aatgatcggt aattcattgt tgtataggtc tgccccttca
     8881 ttgatgccat tgacgatggt atccatattt ccattcagca ttgtattggc atcatggatt
     8941 tcttgtccaa ttgcaggcat ttctttttga tatttttcta aaattgccac tgcattcgtt
     9001 acagttgctt tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc tttgctcagt
     9061 gcgccttgtg cagtcccaat cgtatcgatg acttggtcta gtagagaagt gacagtggaa
     9121 ctgatttgac ctgtattgat accagcaatg atgccattga tattattaac aacggcatca
     9181 atttgatcta gcaattcatc aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa
     9241 gcagataggc ggttttgcag attgcttagc aagggtttta attgatttag ctgatcaata
     9301 attggctgta atttcgtatt tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt
     9361 ttatcgattg cattttgttg ttgagatatt ccgtcaataa agccgttcaa tacattgctg
     9421 atgtttttct tttcatcatc agtcagctta ccatcttcaa tagctgttct tacagtatct
     9481 attaacgaac ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac atctacactg
     9541 tttgtgatgc ttggtatcgc atctttcagc tgagaggcac ctgattttgt ttggtttgct
     9601 agatcacttg cctgattttc taaatttttc acatctggca agatatcttg tacttgttgg
     9661 atgatcgtca agccttgttt tgcttcatcg attccttggt tcattgtctc ttggatcgaa
     9721 gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat tttggatctc tgggattttt
     9781 tcttgcaaat caaggatgat ttttgcttga tctttcaact caggcatttt gtcattcaat
     9841 gcaacgactt tttcacccat ctcatctact ttagggatgt aatccacgaa ttcatttgct
     9901 ttattcagtt tttccttgat ttcaggtagt tgactttgta attcgaggac ttgttttgtg
     9961 tagccgtcga tcgtgtccaa attttcatct gttgataaaa tcatatcttt aactttattg
    10021 atacttacta ggttcgtatc gatgtcgtaa cctatctcat tgaatacttt caataaagtc
    10081 gaactggctg ttttgataaa ttcatttgtg atttgcgatt ggatcgaact tgctccttta
    10141 tcggtgattt tcggtgcaat cgcattgatt ttttcattta ccgtatattc gatttttggt
    10201 ttcttgatat caccactagt aaaactcagc agatcttctg agaaatcctt tggcaagtaa
    10261 atacctgcgt aatactttcc ggaacggact ccgtcttcta attgttcttt tgaatcaacg
    10321 aattgccatc ctaattgttt atttttatga agcgattcga tgacttgctc cccaatggcc
    10381 acttctttac cttgaaactc tgctggcttg tctgcactgt aaacagcaat tggcagctct
    10441 ccagtatttc catatggatc ccataatgct ttgatgttaa accacgcata tagcgaaggg
    10501 atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag
    10561 tcaagcaaat ataatcgaag cgtatttttg attgactgca tagttctccc ccttcttctt
    10621 ttttcagctg aattacttta ccatgtattt catacttttt tcaatcggct tttttcaaaa
    10681 aagtcgaatt tttggacata tcacggcaat tgtctcattg taatctctta catattgaga
    10741 aataattaag gattacagcc tttactttat catgaaataa aagggaaaga taattttatg
    10801 ccaaagtttt tatctattat acgtatacgc aaaaaagcta aaacattttt tgtctttgcc
    10861 cctacaaaaa tgtttcagct tttttcgttt tttattcctg tatttcttat ttttcgaatg
    10921 acaggatgat tttcccagca ggaggatgtc cttcaactag ctgatgtgct tggatgacat
    10981 gcttaagtga agcagacaga ttcattgaaa taaaaacatg aaaggctcct ttttggtagg
    11041 ctttgattat gccagcaaag gcttctgcat ctagatattc ctttcttgga acaaacgatt
    11101 cataaaagcc ttcctttttt tgccgcaaat ctagatctgg gaggtcattt aatgcaacat
    11161 acctgccgcc tggtttcatg atctggatgc cggtttctcc tttgatactt cctttcgtcg
    11221 catcgattac aaggtctgcc tgatcagcaa attgcagacc tggatttgtt ttatcataag
    11281 cagcaaatgc tgaagctcct aattttttta ccttttcctc atttttactg gatgcactgg
    11341 ttaagattcg gattcccttt tcatggagaa gctggatcag acttgacccg accgcacctg
    11401 atgctcctag tatcattaca gtatctgtcg gctggatttc cgtcaaatgg ttgattagat
    11461 tataagccgt aattcctggc gtgaccattc cggctgcttc ttcccaactc attttatctg
    11521 gaatttttgc tacttttgct gatggcaaca cgacttcttc tccgtaagtc ccgccgactg
    11581 catggacagc tactcgatca cctacatgaa cagttgtgac atctgaacca atctctgtca
    11641 caataccagc gccatcatta cctggtacat aagggaattt caatgttcgt atctctttca
    11701 tttctcctaa acgcaaggca acatcataag gattcacact gaatgctttt atttctacac
    11761 gaacatgtcc agaaccgact tctctgggat gagcatcgat ttcttcaaaa acatcttgtg
    11821 cttctccgta accgttaatt gcaaatcttc gcatggtcac catctccctt tttctttaag
    11881 tttacgttta tgtctttatt ttgacaaaaa aaatgtcttt tcgttttggc gcatgattca
    11941 aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa aagcgtaaag
    12001 attacgattt acgctaataa aaaataattg gaggaatttg ttgatggcta aaaaatcaaa
    12061 gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt tacgccagga
    12121 attaaaagcg caaaaagatt acgaaggctt gcgtaaactt cctttagatg ctcgtccaga
    12181 acgattgaaa aatcgtgatt taatcgatgg acgccctcgc ggatacatgc gtaaattcgg
    12241 gatgtcccgt ataaaattcc gtgaattagc acataaaggt ttgattccag gagtaaaaaa
    12301 agccagctgg taatccaagt ctggctgtaa tgtaaaggag gagggcgcat ggaccactcc
    12361 ttggatcatc aaaaaagata cgttcgcttc ttctagcaga aatgctggta ctagaagcag
    12421 acgtatcttt ttttacgcac agctggtcct tttttatatg taaaggctgc atggttactt
    12481 cgctatattt atttagtaac aggagtcagt gcttcgatac ccacactaat tttcaatgct
    12541 cggtcaactt ctgccataat gggtcgatcc aaatggcaga ccttttcttt caatcttgat
    12601 ttatcgatcg ttctgatctg ctccaacagg ataacggagt ctctttctat tcctgttcca
    12661 gtagctttga tcccaatgtg agtcggaagc ttaggtttag ccatttttgc cgttatggcc
    12721 gcaacaatga tcgtcgggct aaaatggtta cctagattat tttgaataac caaaactggg
    12781 cgtgttccgc cttgctccga tcccacgact ggagaaagat cagcaaaata aatatctcct
    12841 ctttttacca taggatcctc cttattcttg atattctctt ggtatccgct gtcctaatcc
    12901 gcaagccact tcatagtgga tcgtacctaa ttgatctgct atatcctgca tagtgatttc
    12961 tttgtctgca tttttgccga taagcgtcac ttttgttccg acaggaaatt cttgaggcaa
    13021 tcggatcatc agttgatcca tacatacacg cccaatcgtt tcacagtagt tcccttctac
    13081 caatagagaa aaaccctgca tctttcgtgg ccagccatct gcataaccga tcggtatcgt
    13141 tccgatccac tctgcttcag gagtgatata tgtttctcca tagccgattc cttcacctgc
    13201 tgggagtttt ttgacttgaa tcaattcaga aaccagctca agtgctggct gcaatggata
    13261 aacttccggt agtgcgtgcc cagagggatt aagtccgtac attgctactc cataacgaat
    13321 catattgccg attgtttcat catgccataa agccgttgca ctattgctga catgtacata
    13381 tcgaggaagt tcgggaagtt ttttcagcac ttctataaag cgttcttttt gcagattcca
    13441 atatgtatca tctgcctgat ctgctgtaga aaaatgggta aataaacctt cccattctaa
    13501 tacacgactt tctttgatga atgcagctgc ctcttttact tcttcaggtg tacaaaatcc
    13561 gatacgaccc atcccagtgt ctgcttttag atgaatcgaa agaggcgtct ctagcttact
    13621 tttctctaaa acagcagctg ctgcttgcaa ccattcccgt gtacctgcag ttactgataa
    13681 atcatgagca acagctacag tcagtgcgtc gtaaggaacc atattcaaga taaggatggg
    13741 ctgagtaaat ccagcttctc tcaactcgac tccttcatct aaatttgaaa cacaaaagcc
    13801 cgaagctcca cctgcaactg ctgcctcagc tgtttcaata gctccatgac cgtatccgtt
    13861 cgcttttact actgcaaaca attcttttcc ttgcggaagt cttgcaactt catttgctac
    13921 attttcagtt atagcttttt tatgtatcac tgctttcgtt gggcggtgcc aagcgacaac
    13981 catcgttttt ccttctttct agtcttccaa aataatttgt gcaaaagctg tttcatttgt
    14041 atgagtaata ctgacccata cattgccttc atgaggtgaa cgcgtcacaa caggctgacc
    14101 attttcattt tttaaaatct cgacatcttg aaaggtaact tttccgatcc cagttcccca
    14161 agctttagag aaagcctctt tgcaagcgta acgcccgcct aaaaattcta cttggcgatg
    14221 aaaaggcaag gactggaata gtttcatttc atcagaggtc aagattcgtg caatgaattt
    14281 tggtttttct tcgatcagcc gagtgatcct aggaagttcg actgcatcga tcccaattcc
    14341 ttttatcata tgcttttctc ttttcaataa atttagaaca ctctcattct accaaattat
    14401 aaccattttg ccacaaataa gtccacaaat aacaaaaatg aaagtttctc ttaaaaaaga
    14461 gaaactttca ttttatgttt aatcgttatt tgaacggatt acaaatccgc gttttttatc
    14521 attgtgacgt ttaccgccat ctttttggct gttattattt ttgctataac cgccttttga
    14581 tttgtttcca cgataattac cgccgtcacg acgattacgg ttgtttccgc caccacgttt
    14641 gccattttta ttgtatcctt ttttctgcat tggcaatggg cgttctggtg tgatcttcac
    14701 tggcactgca tcagctggat cttttgctgt cgtttttagc aaaagtgcaa ctaaatcttg
    14761 tgcagaatat tcttctaata atttatctgc tgtttgcagg tatttatcta gaccattttc
    14821 tgcaagtttt gtttcgattt gttcaactgc agcacctaat tgacctttga atgcttcttt
    14881 ttcggttggt ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa tcacatgcaa
    14941 gtagtccatt tcatttggtg taacgaaagt cacagacata ccgccttttc cggcacggcc
    15001 tgtacgtccg atacggtgaa cgtagctttc tggatcttga gggatatcat agttgtacac
    15061 atgagtgaca cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta caaggatatc
    15121 taaatgaccg ctcttaaatg aacgcaatac gctcattcgt ttttgttgag acaaatcgcc
    15181 atggattcct tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt cgtctacacg
    15241 gcgttttgta cgtccgaaga cgattgttaa ttcaggtgtt tgaacatcaa acaagcgagt
    15301 catgatatcg aatttttcgt attcttttgc gcggacatag tattgatcga tcaagtcagc
    15361 tgtcatttct ttcgctttga tcttcacatg atgtggattt ttcatgaatt taacgccaat
    15421 attcttgata gctggcggca tcgttgctga gaaaagtaat gtttgtcttt gatcaggaac
    15481 ttgtgaaatg attttttcga tatcttccaa gaagcccata ttcaacattt catctgcttc
    15541 atccaaaact aatgtttgaa cggttccaag tttcaatgtg tggcggttaa tgtggtcaag
    15601 catacgtcct ggtgtaccca caacgatatg tggacgatct ttcaagccac ggatctgacg
    15661 gccaatatct gctccgccgt aaacggcttg tacacgtact tttttgtcgc gccctaaacg
    15721 atataattct tcttgtgttt ggatagctaa ttcacgtgtt ggtgcgataa ctaaaccttg
    15781 taattcgtga cgatctggat cgattttttc taacattggt aatccaaatg cagctgtttt
    15841 ccctgtacct gtctgtgctt gtccaataac gtcttttcca gctaatgcta gtggaatcgt
    15901 ttctgcttgg ataggcgttg cttcttcaaa accagcgcgt tcgactgatt tcaacaattc
    15961 tggtgataat tctagttctt taaatttcaa atgaatcctc ctatatggtt gttaactgag
    16021 gatgtagctt ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt ttctttttga
    16081 cagtggccac gcacgttttt atccattctt agcccctgtg gcaccaccga aaaatcactt
    16141 tgcgaagttt aggcattaga aaagccaaac gggtcgcgtt cagctttatc attattatga
    16201 catctggctt caacaagttg tttctatggc aaaaccagtc caatttctct tcatctttcg
    16261 gcaataggtt attctaacat agtttttcgt tttctgcaag tttacagatt tttatttcat
    16321 ttgctgaagt tgttctacca cttcaaataa gcccatgctg ttgctgcctt ttagcataat
    16381 actatctgtt gctgcaagat tttgtttgat agcatcaatc aatttattct tttctttttc
    16441 aaaatagaac acaggtagtt gggggtattt ttggttcaat tcttctttta gtgcatgcat
    16501 ttgttcccca taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt cactcatttg
    16561 cgcatgcata tttaaagact ctttccctaa ttccagcata tctgctaaca cagcgattct
    16621 gcgtcctttt gttggcagat ttttaaaagt atctaatacc agtcccatgg ctgttggatt
    16681 tgcattgtag acatcgctca aaatagctgc accatttcct gccgttagcc attcggttct
    16741 gttttgagtg atagaaacat gggcgagccc ttggcgtatc tcaggtatgg acagtccaaa
    16801 gaagcgcccg atcgtatacg caatcaaagc attcgttaca ttataacttc caggcagcgg
    16861 gatcgtaaat gtttctcctt ctactacaaa atcggtttgt gcttttcctt ttgctgtcac
    16921 ttccgcatga acatcgccat ttccaatacc gaatgttgtg actgtttgta ctaatgttgc
    16981 cgttaaaggt gtcagcaacg gttcatctgc gggcacaacg agtaatccgt ctgatacaag
    17041 tccgtcagtg atttccattt tggctttagc gattccttct cgcgacccaa gattttctat
    17101 gtgtgcttca ccgatcattg tgattgctgc agcttttggt tgagccataa gtgaaagttc
    17161 cgtgatttca ttggcgtgat ccatgcccat ttccaaaacg atcttttctg tatcttgggg
    17221 catgtgcaaa atcgtgtaag gaagaccaat attattgttg taatttcctt gcgtcttata
    17281 tgttttgtat ttttcagaca agacagacgc ggttagatct ttcgtggtgg ttttaccatt
    17341 gcttcctgta attgcaacga cttctgcgcc tactttttcc aagtaatagg ttgccaattg
    17401 ctgcatggct ttcaaaggat ctgctacagg aataactgga aaattttttg gagcttgttc
    17461 aacaggcaag ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct ggataaattc
    17521 atgtccatct cgcgtaccag caagaggaac gaataaactt ccttcggtta tcttccggct
    17581 gtcaaattcg acattggtaa tctttgtcag tgcagagcat tcgctgccca cggcttgtgc
    17641 aatttcttgt attgttagat tcattgagat ctctcctaaa aaaggttgtg aaaaaagcgt
    17701 ttggcctaaa cggcgggttc gcatgactct cacttccaag taaatttctc tattcgtatt
    17761 tagaagctag aatgaacgcg gttacgcaat cactccagcc tcttctttat ttctctttat
    17821 tcattttttt caaaaaaaga ttgacgctga tggtatcgat tcattcctaa ctggatcagt
    17881 tcttcaatca aatcaccgta tttcaagccc atattttccc ataagagtgg gtacatactg
    17941 aactccgtaa atcctggcat agagtttaat tcattcagga ataattcatt tttatttgtc
    18001 aaaaagaaat cgcaccggct caatccgctt ccacctaaca tcgtgtaagc taacttcgcg
    18061 tactcttgcg ctttttgata aacttcttct ggcacttcgg ctggaatctg catttcgatt
    18121 ttattattga tatattttgc ttcataatca tagaatgcta cgtcttttac gacttcacca
    18181 ggcaaagtcg tccgaacgtc ttcatttcct aatacagcaa cttcgatttc gcgcgcttca
    18241 attccttgtt caacgattgc tcgagaatca tactgatagg ctgttgctaa agcattttgc
    18301 agctcttctc ggttttctgc ttttgtaatg ccgacactag aacccatatt cgccggtttg
    18361 acaaacatcg gataaagcaa agaaccttca cattgatcaa atactttttt aggattttct
    18421 ttccattgat tcttaagtac tggtacataa ggaacttgcg gcacaccagc agcttgtaaa
    18481 atatacttgg tcatgatttt atccatggca catgcactgg tcaatacgcc tgcgccgaca
    18541 taaggcatat tcaatgtctc taagaagcct tggatcgttc catcttcccc gtttggccca
    18601 tgtaaaactg gaaaaacgat ggctccttct tctttgattt cgcccggatt gatcactttt
    18661 cctgtaaagc cttcctctgt ctgtccactt gggtcccatg aaagatgcaa gacatctttg
    18721 ctggcaggtt tttctgttag taatggacct ttgacccatt gtccttcttt tgtaataaat
    18781 acgagttgaa cttggtaata attataataa atggcattta aaactgaaaa tgcggaaaga
    18841 atggacactt catgctctgc gctgcgtccg ccatatagta aagtaatctt caaaagattg
    18901 tcctcctgta ttctaaatcg ttcagtgatt tactctcata ttttagcaca aaacaaaaat
    18961 aaacgaataa cttatttata aaaaaacgag ccagcttcta aaagaacgag cgtcatttat
    19021 tttttgtcag ctagattatt tgtcccgttt tttataaaaa gttatgataa agaaaaggag
    19081 ggatgatgat ggcgcaaaca aatccacgca gccgcttggc agaaatcatt cgagtgttca
    19141 tcaattacaa tgtagtgcca aactttgtac agcaaaaaaa tcccgaacag gtaaaaaaag
    19201 cctttgaaga actaggcccc accttcatca agataggaca gatgctttct gttcgagaag
    19261 atttgctttc ttctgccttc actcaaacat tcaaaacatt acaagatagt gtacctagcg
    19321 acacattttc aacggtcaaa aagacgatcg aaacagaact ttctctttca ttatcagata
    19381 tattcgatga tttttcaaaa tcgccttttg cctctgcttc tatggggcag gctcatcgtg
    19441 ccaagctgaa gaacggtgac gcggtagtcg ttaagatcca acaccctaac atcgccgaag
    19501 agatccgact tgatcttcaa ctatttgaac gagcgatccc tttgatcaaa tacattcctg
    19561 aaactagcgt agttgatcta aaaggggtac tccaagaagt aaaacgttcc ttgatcaatg
    19621 aaatggactt cttaaaagaa agtcaaaatg gcgaacaatt ctatcaaaag aacaatggct
    19681 ggaaagagat ccgcagcccc aagatttatg acgctttttg ttcgaaaaaa gtgatcgtga
    19741 tggaagaaat gtctggtaaa aacttgaatc atttaatgaa cgccgaaaac aaaactgaaa
    19801 cttttatcac tggaattcaa aacaagcagc tgaaacaaga agttgcaaag cttttagttg
    19861 agaactttat gaaacaggtg tttgatgatg gttttttcca tgctgatcct catccaggga
    19921 atctgctatt ccatgtacta acaaaagaag aacagacaca agctagccga aaaactgaaa
    19981 cagtccatga aaaagagttc ggttcctttg cttttcgagc ctccacttct gcagaagacc
    20041 ctgttgcgcc atataccatc aactacatcg attttggtat gatggggcat ctttctgcag
    20101 gacttaggca aaaactcaca caagccgttt tagcgctgta tacgaaagat gcttatcgaa
    20161 tcgaaaaagc ggtccttcga ctttgtcagc aagaaggctc gtttgatgaa agtcggttcc
    20221 accaagaact gacaagtttt cttgaacagt attatgattc tcctattgat gaaatcaact
    20281 tgcaagaagt attcacacat gtcgtgacga tttgtcatca aaataatctg cagttcgatc
    20341 gtgatatcac gcttttgctg aaagcatttg ggacattaga aggggtgatc cgcgtcctcg
    20401 atcctgaagt ttccctcatg gaagtcgctt cgccttttgc tcaacattat tttctcactc
    20461 atctggatgt agaagataca ttgaaacaaa gcggattaga cttattggaa gggatgaagg
    20521 ctgcgcctaa gatccctcaa cagctccacc atctattaga aatgtggact tcgggacaag
    20581 gaaaagtcaa tttggaattg aaaaagcaag acaaattatt gagcaggatc gaaagtatga
    20641 tcaatcggct tgttttcggt atgattttag ctgcattgat cgtagggtcc tccctgcttg
    20701 tccaagcagc acctgtagaa aacgcagagg tcgtttcctt attagggata tttacgtatg
    20761 ctattgccgc attcgtcatt atttttttgg caatcgatgc attgatccaa atgtataaga
    20821 agaggaaaaa gtagaaaaag gttgtgacag aaagaatcag cttcaagaaa taagaagggg
    20881 ttttacataa attgtttctc aaatttttgt gaaaattcgg cttatttttc gaagaggcta
    20941 cttctgaaac accgagaata aaaaatgtga aaaagctatt ctgcacccag taataagaac
    21001 aatcaaaaag ggaacatgga acacatttat cctgtttccc tgttcccttt ttgatgtatt
    21061 cgagcataaa taaaatacct tcgttaccta ctatcttctg tacttggtaa accttagtca
    21121 attgcctttt ttaacgcttg acgaaaagac atatgatctt tctccgtaaa gggttttgga
    21181 ccctttgtcc ttatgccagc ttttcttaac tttccactat gataacgttg ttctagaagc
    21241 gtgccgatat tcgcttcgtc aaattctttt ccagagtgat ggaacactcg cacacccttc
    21301 ggcaatacaa gtgaagctaa accataatct tgcgtgatca aaaagtcatt cttcttaacc
    21361 ataccgacga ttttataatc cgcactatca gctccgcggt ctacgtagac gatttggaca
    21421 tgattagcgt attctttggt cgtataatgg tccacactgg tcacgataac cacgtcaaga
    21481 ccataggatt ctcctaattt tatgacatct tcttttactg gagaaccatc tccatcaatg
    21541 aatagcctca ttctttttcg ccttcttcca taaatgataa acagttacta atccgctcag
    21601 taaaagacca acattgatcc attttgccaa gataagcagt tgtacacttt ctatcggcac
    21661 tgattcatgg ttcagcgtga tcaataatag atggattggc tctcccattt cttttgctaa
    21721 aacaaacaaa agcgtcatca ataaaaactg gtggatcaca ctccctttat tttgagtagt
    21781 atacttgaca acgatccggc gatccattgg aatagctgta tcagtttgat aatattcctt
    21841 atttagccag ctgcctgtta ctacgtgcca gaaactaagc agaaagacac ctatcgcaaa
    21901 accatttgga atcaagagga ccaataaaga agaactcagt ccaagcaatt gcatagccaa
    21961 tggagaccat gttaaacgtt tcaaaatcca tggtccgact atttttgctg caatcaagcc
    22021 tgctgcataa ggcaaataca taaaaatcga aagtttttct aatccgtaca gtatgccaac
    22081 ataaaaactg ctaaataaaa aaaggaaatt ccctagcatt ccatttagaa aagtaaacag
    22141 atttagccag cttggcaact tccattcctt cttctggtgt cctaaaacaa acacgaacaa
    22201 gatgaacagc agcaagaaac cataaatagc aagatcaaaa agcctttcat cgaataacaa
    22261 tcgggcattt ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa caagctctcg
    22321 cgtagctacc agatttcttt tgatgtcttt gaaatccagt tcgtaacgcg gataatgact
    22381 aaccgtatgg taggccatga cgtaaaaaag tgtataaata gtaaagcttg ctgcaatctg
    22441 agtcggtgca ggcaactcaa tcgcttgata caggagaaat agccagatag ctgcaaataa
    22501 gtactttttc ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat tgaccgtggt
    22561 attagcaggt acaagccatg ctgcactcag ccctagcaaa acacttgaaa aataaaataa
    22621 aggaaaataa aatacgccta atattccaag caatgaacca gcaccaccaa tcagcagagc
    22681 gatcatcagc aacgtgtaac tatctagacc aaatcgtaga ctgttgacta aaaatagtcc
    22741 agtcatccga aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat tatgcgagtc
    22801 tgttagtccc aaaaataaaa gataaggaat aaaaataacc gtgttactta agataaaggg
    22861 cgttgtttgt gtaaaattac gcccaaatcg tttgagtttt tcgaacatga caatggctcc
    22921 ttaaacagtc ttataactaa tctgctcttc gattttagca ttaaagggga aataaaccaa
    22981 ttttacagaa tatattcttg taaaaaaaaa tacagaaaga agcataagct tctttctgca
    23041 tttttacggt acttgattac tcaagcagac aagcccttct ccacaaaagt cttgcccgac
    23101 acccctgcat ccggcaggtg ataaagatag aaatcaatta tcctgtgcct ccaaaatcaa
    23161 ttaggagctc tgtaaaccag agtccttctc gacccgtcgc ataaatttac tttaacacag
    23221 ttcctatgtt tatgcgagag cttttttcat tttataatcg accatttgaa atccttgacc
    23281 gatatctgaa ataagttctt ctgtaatctc aaatccttca tgcttataaa cctcgattgc
    23341 ttgatgattc ccctgattca ctcgcaaaaa gatctctctt tcctttgttt gcgcagcaag
    23401 ttcttcatac cagcgaaaaa ctgaagaagt caatccttga ccacgtactt tatttagcaa
    23461 atataattta cttaaataaa tttttccatt tatctcttca taagcagtgt aaccgacagt
    23521 cttttcttcg ctttttaata aaaagtagga cacaccttct gtcagttctt tttgaatctg
    23581 tttttttgac tgataagttt tcagcatgta atccacttgt ttttgaccaa tgatcggcac
    23641 aaaagcttcc ggccatactt cttgtatgac tgccatcagc tcatccagtt cattttctgt
    23701 tacttttatt ttttccatac acccacgtcc ttttttacat tataaatgac cttgcgcttg
    23761 atggaataaa aagagcatga tcatgctata cacacccacc gaccatggtt tcaagaaaat
    23821 cacaatctgc ataaattccc taaaagataa tttcgtcaaa ccagctacta gacagatcaa
    23881 gtcgtctggc gcaaagggaa gcagcaaggt aacaactagt acttttttaa tatctttcgt
    23941 ttttcttgtc agcaattcat caaatttctg atactttttt ggcgataaaa taagctggac
    24001 aaaggctacg ccatagtaac ggacaagcaa aaatccgatt gcttccccaa tcactaggcc
    24061 aatacaacta tataaaagcc caaatccatt gccaaacatc agcatgcccg ctactgagga
    24121 gatccctccc ggcaaaatag gaatgatcac ttggatcact tgcaacagaa caaatatcat
    24181 cactgcatac ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg atcgaaaaat
    24241 cccatgatga taagcataaa gaatcaacac aaagaaaaaa aacagaccaa taatgggcat
    24301 gatctgaaga catttttgta aaactgatcc ttttttagac atctatattc cccacatctt
    24361 ttttcattta ttaaaactgc ctcct
//
LOCUS       DACEZN010000023        23873 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEZN010000023 DACEZN010000000
VERSION     DACEZN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.023"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            476..1339
                     /locus_tag="IXH60_000741"
     CDS             476..1339
                     /locus_tag="IXH60_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1989703.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="IXH60_000742"
     CDS             complement(1468..3090)
                     /locus_tag="IXH60_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1989704.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="IXH60_000743"
     CDS             3339..3860
                     /locus_tag="IXH60_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989705.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="IXH60_000744"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="IXH60_000744"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1989706.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="IXH60_000745"
     CDS             5941..6759
                     /locus_tag="IXH60_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1989707.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="IXH60_000746"
     CDS             complement(6862..7059)
                     /locus_tag="IXH60_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989708.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="IXH60_000747"
     CDS             7228..7806
                     /locus_tag="IXH60_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1989709.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8851..9354)
                     /locus_tag="IXH60_000748"
     CDS             complement(8851..9354)
                     /locus_tag="IXH60_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1989710.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9714..10739
                     /locus_tag="IXH60_000749"
     CDS             9714..10739
                     /locus_tag="IXH60_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1989711.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11086..11964
                     /locus_tag="IXH60_000750"
     CDS             11086..11964
                     /locus_tag="IXH60_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989712.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12440..12556
                     /locus_tag="IXH60_000751"
     CDS             12440..12556
                     /locus_tag="IXH60_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1989713.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13484..13822
                     /locus_tag="IXH60_000752"
     CDS             13484..13822
                     /locus_tag="IXH60_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989714.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13916..14146
                     /locus_tag="IXH60_000753"
     CDS             13916..14146
                     /locus_tag="IXH60_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989715.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14546..14917
                     /locus_tag="IXH60_000754"
     CDS             14546..14917
                     /locus_tag="IXH60_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1989716.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15191..15883)
                     /locus_tag="IXH60_000755"
     CDS             complement(15191..15883)
                     /locus_tag="IXH60_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1989717.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15886..16671)
                     /locus_tag="IXH60_000756"
     CDS             complement(15886..16671)
                     /locus_tag="IXH60_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1989718.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16908..18308
                     /gene="lacG"
                     /locus_tag="IXH60_000757"
     CDS             16908..18308
                     /gene="lacG"
                     /locus_tag="IXH60_000757"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1989719.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18333..18650
                     /locus_tag="IXH60_000758"
     CDS             18333..18650
                     /locus_tag="IXH60_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1989720.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18663..20336
                     /locus_tag="IXH60_000759"
     CDS             18663..20336
                     /locus_tag="IXH60_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1989721.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20350..21981
                     /locus_tag="IXH60_000760"
     CDS             20350..21981
                     /locus_tag="IXH60_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1989722.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22853..>23873
                     /locus_tag="IXH60_000761"
     CDS             22853..>23873
                     /locus_tag="IXH60_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ1989723.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQI"
BASE COUNT         7445 a         4051 c         4819 g         7558 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct ttttttttgt tgggaatccg
     9481 gtttctttaa caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc
     9541 aaaacgactc ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata
     9601 ctaagtttat gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt
     9661 ggcatttcat cttggttcag ctttttttcc aattactagg aaggagggat agattgttca
     9721 agacttattt tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc
     9781 tacttatctt tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt
     9841 tttcatcatt agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc
     9901 tgaaacgtat gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat
     9961 cgacttatgt tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat
    10021 tcgtgttgcg attattccat atttctaaac caggtggctt gttttttgcg atgctttctg
    10081 ccatgggaac gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt
    10141 ttatgggtgt cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc
    10201 ctgagcagac aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga
    10261 tcattgattc tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct
    10321 tgaatctgca taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa
    10381 acttggatgc gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat
    10441 gtgtgtcggc attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca
    10501 cttttctata tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt
    10561 ttcttaatcc gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt
    10621 tgatcgaata tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt
    10681 gggtaatgac ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga
    10741 aggacaaata tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca
    10801 aggtgttcaa aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga
    10861 actttcaaaa aaattttata tttattctat caaaaaattt tatatttatt ctatcaaaaa
    10921 attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa
    10981 gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa
    11041 gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat
    11101 atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact
    11161 ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat
    11221 gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca
    11281 aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat
    11341 gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag aactatctat
    11401 ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc
    11461 tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt
    11521 ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa
    11581 ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca gaataagata
    11641 agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac
    11701 caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaatg gttgaaaaaa
    11761 gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac
    11821 gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa
    11881 agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct
    11941 ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga
    12001 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
    12061 caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
    12121 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa
    12181 cacacagtac gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa
    12241 gagggccttt tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt
    12301 caatcagcta gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc
    12361 atttaaaaca caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag
    12421 aagaaagtgg gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa
    12481 aagcccaaat atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa
    12541 tagtgggaaa aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc
    12601 ggctattttc aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc
    12661 atttttaaca tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat
    12721 tccagttata tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg
    12781 tagttatttt tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac
    12841 cttttaacac ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca
    12901 taatcaaagg ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt
    12961 tgcaaataac cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc
    13021 agctcactcc atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg
    13081 aaaaagttaa ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta
    13141 ctattgctag aataacatca aggaataata agttttggtt ttcagataat agtcgataag
    13201 ttgtgtattg gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc
    13261 actattacaa gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa
    13321 atgaatagtt gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt
    13381 caatttgtga ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat
    13441 tgtagtgttt attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc
    13501 aagtgaggaa catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa
    13561 tattgttccg gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat
    13621 aggatggtat atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac
    13681 agtaggctta gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac
    13741 tgatgaaagt gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa
    13801 aaagtatata ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa
    13861 aatttactag atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct
    13921 ggtagaaaag ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga
    13981 aaatgagtgg aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt
    14041 ttacagagaa ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt
    14101 agctgaagta gaagagtttt tggattatgt tagtatgcaa cagtaaggga gttcttatta
    14161 tgaaaaattt tgacacatat tatgttattg ttttttcgat atctttgctg ttaatattac
    14221 taactttaat gcagtataaa gagtatccag agtattttat tttaactttt gtatatttag
    14281 taagtagcta cacacttcta tataaattca gaagttcttc tagagtttct aaagtttgtt
    14341 tcttaattag cttattgatt ctataatatt tttgatttat ataggaaatt aaaaatatta
    14401 tagaatcaac actcagagaa cgggttggtt tttaatagat ttaataagta ctgataatga
    14461 aatttctcct ggttgggaaa acgaagttgg ttaccctgta gaagaatttt ttcaagatta
    14521 ctttgcggag tatgccgata ggataatgag agattacgta acagataaaa ctcaagaagt
    14581 catcaatcaa tcccagtcat taaatgaatt atctgataag gcgaaagaag aagcagttgc
    14641 cattatttct tcagggatta gtgactatat tgatatgagt ggtgaaatca attatggcga
    14701 taaagacaaa attgtacgca tgataaaagc ttttatcaaa acttttgatg tgaaggcaga
    14761 tcttaagttt aatgatactt acctagtagg aaaattaatt aatatccttg ggacagagaa
    14821 agatacggaa acgtttattt cctctttgtc tatgttgttt acagataagc atttttcaac
    14881 tcagcttggt ggagaggaac tagtggaaat cttttaacca ttacgcccag cattaataaa
    14941 agtatatcaa aaaagtactc ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa
    15001 cactattttt aatgttaatt atagatagag atgaacgcgt catatggtta aaagaatgaa
    15061 aaagtcaaag atacactgct atatttcact aaggatatag cagtgtatct ttcatgtcgt
    15121 ttcactggca gttttttata aaaggaggtg tgcatttcag ttgcttttct ttggtagtta
    15181 gttgaatcgt ttaagaatta acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa
    15241 aatctttgat ggttccatct ttatttagct gtgcctgttc aaatactgtt ttgtttttac
    15301 cgaatggatc aattgcaata attgttccga tccccgcacg tttggcagca agtaatcctg
    15361 agtaagcatc ttcgataaca agacaatcag ccggagctaa gctcagtttg tctgccgctt
    15421 taagaaaaat atccggttgc ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt
    15481 cccagtcaaa ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga
    15541 cagtagccgt ggcaatcgtt aatggaatct gttggtcagt cagctggtca agagtcgctt
    15601 caagcccttt tgtcaactga agctcgtttt cattttgcaa gcaaagcata cgataatagc
    15661 tttctttttc ttctgacaat acggcgatct cagaggcggt caaatgctct gaaatgaaat
    15721 gtttcaaaat ctccgtattc gtccgaccat gaatattttt taaaatatcc tcgtctgata
    15781 aaccatcttt tgtatgatca tgtatcatat gcagccaagc agcttcatgc agatgagagt
    15841 ccaaaaacat tgttccgttg aaatcaaata taatcccttt catagttacg ctcctatagt
    15901 ggtattttcg gttgaatcag caatttgcag atcgacatat tgtgcatagt gctcttgaac
    15961 ctcccgcgag atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac
    16021 ataaaaatca tctcgattga attttttatt atcaacgagt agatatttct tacgggaatt
    16081 atttagagcg atctgctgtg cttctccttc ttcggtactg taagtagaga tcgcttcgtt
    16141 gtgaacgcca tttgaactga taaatgcttt tgtaaacttt agtcttctta aattgtcgtt
    16201 ggtgatcggt ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa tcagtagaat
    16261 ttctggtcca tctgcttgag caagaatatc aaaaactgga taattatttg taatgatccg
    16321 aattttcttg ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa
    16381 aatcgtttct ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt ttttttcttg
    16441 aatctgcaga gtggcttttt cagtgtgaga aagttcttga tctaaagtgt aggaaagact
    16501 ttgagcccct ccatggacac gcagcagctt gcccgatttt tctaattcat ctaaatctct
    16561 gcgaacggtc atatcagaaa catctaattg attgatgatt tcattaacgg taaggattcc
    16621 ttttttattg accatttcta cgatagtaag caatctttct tttttcaaca ttgtcttcac
    16681 ctctaattta aggataatct atcatataaa cggacaacag tcaatctaat tcgaacaaaa
    16741 gtaaacattt agggtggttt ttatgtttgt tacaatagat tttattgctg ttttctggtt
    16801 tttatgatta tttgtgtttg tttttgttga caaatgtttt ggatgcgttt acaatgcaat
    16861 cagttagaaa gagcgaaaac tgattataga tttgaggaga gatgtacatg cttaaattac
    16921 cagaagattt tatttttggc ggagccacag cagcctatca ggttgaagga gccactaagg
    16981 aaggtggcaa aggcgctgtt gcttgggatg actttttaga ggaacaaggg cgattcagtc
    17041 ccgatccggc aagtgatttt tatcatcaat atgcaaagga cattgaattg tgtgaacgtt
    17101 ttggtgtaaa tggtctaaga ttatccattg cttggagccg catttttcct gatggagctg
    17161 ggaaaccaaa tccagaagga atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa
    17221 gaaacgtaac gccgtttgtg acgttgcatc attttgatac ccctaaacgt ttgtttgacc
    17281 atggagattt tttgaatcga gaaacaatag aagcatttgt ttcctatgcc attttttgtt
    17341 ttcatgaatt caaagaagtc aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa
    17401 ccaatcaata tttgttaggt gttttcccac caggaatcaa atatgatttt acaaagatcg
    17461 tcgcctgtct tcataatatg atggttgctc atgcccgtgt agtgaactat ttcaaagaaa
    17521 acgaacttcc gggtgaaatt ggtgtagttc actcattaga gacgaaatat gcggcaacag
    17581 acgcgccgga agataagcat gctgcctttt tagacgatgc attgtcgatc cgtttcttat
    17641 tagatgctac ctatttaggc tattattcaa ctgaaacctt gactgctctt gatgagatat
    17701 gtgaagcaaa tcaggccagc tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag
    17761 catccacgcg taatgactac ttagggatca atcactacca atgccatttt gtcaaagctt
    17821 acgatggaga aaatgcaatc catcataacg gtacgggtga aaaagggacc tctgtttata
    17881 aagtgaaggg aatcggagaa cgtatttata aagaaggaat tcctcgaact gattgggact
    17941 ggctgattta tccagaaggt ctatatgact tgttgctgcg aattaaaagc gattacccac
    18001 attacaacaa aatctatatc actgagaacg ggatggggta taaagatcag tttgaagatg
    18061 gcattattat ggatcagccg agaatcgact atttgagagt ctatttggaa tccttaagca
    18121 aggcgataac agccggggta aatgtgaagg ggtatttttt gtggtcgctt atggatcttt
    18181 tctcatggac aaatgggtat aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc
    18241 agaagcgtta cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa accaaaacga
    18301 ttatttaata agtgataatg gaggaagaaa agatgaataa agaggaatta caaatgcttg
    18361 gttttgagat cgttgcatat tcaggcgacg ctcgcagtac attattgaaa ttattgaaag
    18421 aagtaagaca aggaaacttt gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt
    18481 taacccttgc acataattcg caaacaaaaa ttttagcgga agaagcatct gggaaagaga
    18541 tggagatggg attcattttt atccatgggc aggatcattt aatgaccact ttattgctga
    18601 gagatctgat tcaagacttt attgtactgt acagacagaa tcaaggatag gagaagaacg
    18661 ctatggatgg aatcattaaa caaatcgaaa aagggaaacc attttttgaa aaaatctcac
    18721 gtaatagtta tttaggagcc gttcgtgacg gatttttaac agcgatgcca gctattttat
    18781 tttcaagcat tttcattttg attgctgcgg ttcctgagat ttttggttac caatggcctg
    18841 aatctgtctc cacatggcta tggaaagtct ataattattc gatgggtgtt gtagggttac
    18901 tcgtttcagc aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa
    18961 acaaagtaat caacaccacc tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa
    19021 gcgtctctcc gattgaaggt ggattttcaa cggaatatat ggggacaaag ggactactgg
    19081 cttcctttgt cgctgcattt ttaacagtta atttatataa gttttgtgtg ctgaaagata
    19141 ttaccattaa aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc cgagatattt
    19201 ttcctttctc tttttcagta tttgcggccg ttttaattga tttaggcatt cgacaaatct
    19261 tccaaatctc atttgcagaa gcaatcatta cggtgctgca gccgctattt acagcagctg
    19321 acgggtactt aggaatcgct attatctggg gagcgatggc gctgttttgg tttgtcggtg
    19381 ttcatggacc gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa
    19441 ccaatctgca attgttcaat tccggtgaac aagcttctaa agtactgacg gtgggtttag
    19501 ggaattttgt aggaactatg ggaggaactg gggcaacatt ggttgttcct tttatcttcc
    19561 tactctttgc taagtctaag cagttgaagg cagttgggaa agcttccttt attccagtaa
    19621 gtttcgctgt caacgaacca ttgctatttg cggcacccat gattttgaac ccttattttt
    19681 ttattccatt tttgttaaca cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg
    19741 tattgggcat gaacagcttt atgtacgttt tgccttgggc aactccagca ccgatcggtt
    19801 tggttctagg aacgggcatg ggtatcttag cttttgttct agcagcggtt ctaatcgtcg
    19861 ttgacttcct gatctatcta ccattttgca aagcatacga tgctgtctta gtagagaaag
    19921 aagcacaaaa agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg
    19981 aagtacctgt ggcagcgagt caagttatca gcgatgcaaa taaagaaatc aatgtgttgg
    20041 tactttgtgc tggcgcaggg acaagtgcga tgttagctaa tgctttaaaa aaaggtgcat
    20101 ctgaatcgaa agcaccgatc agtgcaatgg cgggtgctta tggttctcat tacgaaatgc
    20161 tgcaggattt tgatatggtg gtcttagcac cgcaagtcaa ttcttactat gatgatatca
    20221 aagaagacac cgatcgctta ggaatcaagc tggttgcgac aaaaggagca gaatatatcg
    20281 gattaacgag agatcctgaa aaagctgttc agtttattct gaaccaattt caataaagga
    20341 ggagccataa tgaaaatggg aatttggttg cgtaaagctg ttttatttgc agctgtgtta
    20401 ggggctttgg gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga
    20461 gcagatattt cgatcctgtc agatatggaa aaatctggtg caaaatatta tgaagacgat
    20521 atttcaaaag atgccttaga gattttaaaa aataatggtg tcaactatgt tcgactgcgt
    20581 ttgtggcagg atccttataa tagtaatgga gaaagttatg gagcagggac aaatgatctg
    20641 gaaacgacga ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt gctgctggat
    20701 tttcactaca gtgatttttg ggttgatcct gggaaacaga atcttcctaa agcatggcaa
    20761 ggtctgacct ttgaagaaat gaatacggca ttgtacgact atacaaagaa cgttctgtcg
    20821 gaaatgaagc agctggacgt ttatcctgat atggttcaga tcggcaatga actgaatagc
    20881 ggaatgctct ggccttatgg caagtcttgg ggagaaggcg ggggagaatt tgatcgattg
    20941 gctgcttttt tgaagtctgg tatccaagct gttcgagaca ctcaacctaa aacaactcca
    21001 gtaatgctcc acttagctga tggtggcgat acaggagcat ttacttggtg gtttgatgaa
    21061 atcacgagtc gaggtgtgtc atttgattta attggagtgt cgtattatcc gtattggcac
    21121 ggttcaatga atgaacttca aaccaatatg aatgcgatca gccaacggta tggtaaagaa
    21181 gtgatcgtag ttgaaacagc ttatgggcat acaacagcga atgccgatac tatgccaaat
    21241 gcttttggtg aagccgaagc agctgcgggg ggatatgaac caacaccgac aggacaagca
    21301 gagtatttgc tggatcttgc cgatcggatt caggcagtac cgaataatcg cggggcgggc
    21361 ttcttctatt gggaacctct ttggtacaac ggaaatgtga gttgggcaac acaagcaggt
    21421 atgaattatc tcagtgttca atccactatg ggaaacgaat gggacaacca agcgatgttt
    21481 gattttgctg gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata
    21541 aatctagtta aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg
    21601 caaacctggt ttcaacaagg aaccgctaag ggtacgatca aaacggaagc gggcactttt
    21661 tttggggact acaaactaac tttttggcag gatcaagcat acgaggcatc agcttatcaa
    21721 aatgttagtg gcataagcaa aggaacttac acattgagtg cctatgtaat gtctggggga
    21781 gagtttgata ctaatcagtt atatatcaaa aattatggtg gagcggaact aaatactgcg
    21841 gttgttaaaa caagtgaatg gacaaaaatt acaatcaaag acattccaat tacaacaggt
    21901 gtttgtgaaa ttggggttta tagcaaggct tcaggaaatg cttggacgaa cgttgatttt
    21961 gtttcattgg tgaaacaata gactgcggat cgtttcgatt caagatcgga ggttcccttc
    22021 agccacatcg ttagcgtaga aggcaggaat cgaacctgca cctcgccttt gccaaaaata
    22081 accagaaacg atttccagtc atttttacca aaacgagtaa agcaccagac ttcatctaca
    22141 ctttgtatta tgctaaagag agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa
    22201 aagttgtgca gataacataa agtttgttta agaaagggta accttagaat tccgtcgtga
    22261 atatgatttg agcacttaga tggatagaaa aaagttctcg gaaaacagtt cgtttttcga
    22321 gaacttcttt ttatgataag aatgaaacaa attaacttat attatagtct cttttggtag
    22381 ggaaccatct tatttttaat gaaaatcaag cattttagct attgaagaat gttgatttat
    22441 agggattccc cctccatatt atgttggctc tttattccca ctctgttttc aacgtaaagt
    22501 gtataaaaca gaatgcgata ctatcaaggg agttaaaaca atatacagaa cagtaaaaca
    22561 ctataaataa aaataaaagt gcaataaaag tgcaattttt gcacctaatc atactctgtt
    22621 tatatatgaa aactagattc tgaatccaat atttggttaa cgagaggcgt tcgaatttga
    22681 acgtctttta tatattactt tcaaataaat atgaggtaga atactatcta ttttcttttt
    22741 aacccaattg ttataatgga gagtgtaaaa tattttgtgt aaatagaaaa aaggaagtcc
    22801 cttctgtaga atagagttac cacaacacat tcacagaaga gaggacttcc ctatgaacga
    22861 ttttactaca gaaattctaa agactctagc gaacaaaggc gatttgaatg aattattccg
    22921 tgtccatttg gagaaagcag tcaatacgct tctcaaaacg gagttaacgg ctttcctaga
    22981 ttacgaaaag tatgatcgca ttggttttaa cacgggtaat tctcgtaacg gctcctatga
    23041 ccgtacggtc aagaccgagt atggggaact tcatctccag attccgcgcg accgcaatgg
    23101 tgagttcaag caacagactg ttcctgctta tagacggacg aatgacacgt tagaggagac
    23161 cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact tgattgagaa
    23221 aatgtatggg cattactaca cgccccaaac catgtccaat ataacaaaat catttacaga
    23281 agaggtaacg gcgtttaatg ggcgggagct tcatgaccgt tatgctgcta tttatatgga
    23341 cgcaacgtat attccgttaa agcggaaaac cgtcgccaag gaagctattc atatcgcagt
    23401 tggcattcgc ccggacggat caaaggaagt attgagctat gcgattgcac cgactgaatc
    23461 catcacgatt tgggaggaaa ttttattgga ccttcaagag cgcggtttga aaaatgtcct
    23521 cctgttcatc acggatggct taaaggggat ggtaggagcg attagtcggt tctatcccaa
    23581 agctcgtttt caacattgtt gtgtacacgt ttcccgtaat atcagtcata aagtgcgtgt
    23641 cgatgatcgt aaggaagtct gtgatgattt taaaatggtg tatcaagcct catctaaaga
    23701 ggtggcgttg gaagcacgtg gtacttttgc ggaaaaatgg aaaaccagct atccaaaagt
    23761 ggttgaatcg attctttcga acgatcactt gctcactttc tatgatttcc ccttggccat
    23821 acgcaagagt atttattcta cgaacttgat tgaatccttt aataaacaaa tca
//
LOCUS       DACEZN010000024        23432 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEZN010000024 DACEZN010000000
VERSION     DACEZN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.024"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            31..717
                     /locus_tag="IXH60_000762"
     CDS             31..717
                     /locus_tag="IXH60_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989724.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            725..1372
                     /locus_tag="IXH60_000763"
     CDS             725..1372
                     /locus_tag="IXH60_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1989725.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1445..2836)
                     /locus_tag="IXH60_000764"
     CDS             complement(1445..2836)
                     /locus_tag="IXH60_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1989726.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            3093..6482
                     /locus_tag="IXH60_000765"
     CDS             3093..6482
                     /locus_tag="IXH60_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1989727.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            6485..7906
                     /locus_tag="IXH60_000766"
     CDS             6485..7906
                     /locus_tag="IXH60_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1989728.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            7903..9780
                     /locus_tag="IXH60_000767"
     CDS             7903..9780
                     /locus_tag="IXH60_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1989729.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            9872..10714
                     /locus_tag="IXH60_000768"
     CDS             9872..10714
                     /locus_tag="IXH60_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1989730.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(10866..11741)
                     /locus_tag="IXH60_000769"
     CDS             complement(10866..11741)
                     /locus_tag="IXH60_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1989731.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            11948..12979
                     /locus_tag="IXH60_000770"
     CDS             11948..12979
                     /locus_tag="IXH60_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1989732.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            13068..13373
                     /locus_tag="IXH60_000771"
     CDS             13068..13373
                     /locus_tag="IXH60_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1989733.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            13552..15567
                     /locus_tag="IXH60_000772"
     CDS             13552..15567
                     /locus_tag="IXH60_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1989734.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            15569..15853
                     /locus_tag="IXH60_000773"
     CDS             15569..15853
                     /locus_tag="IXH60_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1989735.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            15879..17252
                     /locus_tag="IXH60_000774"
     CDS             15879..17252
                     /locus_tag="IXH60_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1989736.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(17335..19350)
                     /locus_tag="IXH60_000775"
     CDS             complement(17335..19350)
                     /locus_tag="IXH60_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1989737.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(19372..20586)
                     /locus_tag="IXH60_000776"
     CDS             complement(19372..20586)
                     /locus_tag="IXH60_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1989738.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            20807..21751
                     /locus_tag="IXH60_000777"
     CDS             20807..21751
                     /locus_tag="IXH60_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1989739.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            22047..22991
                     /locus_tag="IXH60_000778"
     CDS             22047..22991
                     /locus_tag="IXH60_000778"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1989740.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         8001 a         4253 c         4419 g         6759 t
ORIGIN      
        1 cccaattaca gcaataggag gttaataaaa atgaaaaaaa tattaacgat gaaaaaagtt
       61 accaaaacat ttggttctgg tcatacaaag gtctcagctt tgaaagcaat tgatttttat
      121 gtaaaggaag gagaattcgt cagcatcatt ggtccttctg gttcaggtaa aagtactttc
      181 cttaccattt caggaggatt gcaaacaccg acttccggag aaatagtgat caacggtcac
      241 tcactaactg agcttccaga aaaaaaacga gcagatttac gctttaaaga aataggattt
      301 attctccaaa gctctaattt gattcctttt ttaacggtaa aggaacaatt tgaactagtt
      361 gaccaagtta cttcaaaaaa agaagattca acaaaaatcg attccttgct gcaatcttta
      421 gatatccagt cacttaaaaa tagttatcct aaagaccttt ctggtggaga aagacagaga
      481 gtggcaattg ctcgcgcttt atataatgag ccaagcttga tcttagcaga cgaaccaaca
      541 gctagtttgg atacagatca tgcatatgaa gtcgtcaaat tactagttaa agaagctcat
      601 gagaaacaaa aagcgactat tatggttact catgatgtcc gtatgattca atggagtgat
      661 cgcgtttatc gaatggaaga tgggtacttg acagaagaag agaaaaagaa ggaatgaatc
      721 cttcatgaca atgaagggct atccacgtat aatcagagat aaaggagaga tgtggatgcc
      781 aaaagaaact tttttcaatt tatcgaacgc aaagaaacag agaatcgatg acctattact
      841 ggagatattt tgctcacagc acatcagcca agtcaaagtc tcagaaatcg ttgacaagat
      901 ggggatgtcg cgcggtgctt tttataaata ttttcaagat ttggaagatg cttatttgta
      961 tacgatcaaa agcagttctc acatagtcca tcaagatatc ttaaaattca ttagcgaaaa
     1021 caaacaacat ttctttttgg ggatcgaaaa atatttagag tggtgctgcg acttagatca
     1081 cgaaagtatc tattggcaaa ggttatactt gttaacccag cgaaatgatc agcagacaac
     1141 caaacgtaga gaactatcgg ctgattctcc aatgaacgaa aaatggctgg aattattaag
     1201 aatcaatcat ttctccattc aatcgtcgga agaagcagtt agtttcctat acttttccat
     1261 gaccctcgta atgaacactt tgacggatct cattgcaaat aattggacaa aagaagagtt
     1321 gttaaaagac tttcgctata aatctcgttg gattgctgaa ggaatacaat aaaaagaaac
     1381 agctatgtgt atttgtcctg aaatttttat cagggtggtt tatacatagc tgttttttga
     1441 ttattcataa ctcgaataag cggatgagtt cttcttcatt tgcgaatgac acactatcat
     1501 caatcaatat atgaggaaca tgggcattca aaatgggttg attcgtaacg atcagatcat
     1561 actcgagaag attgtatttc gacagctgat cttcagtaat agcagttgtc tcagagtgaa
     1621 ttttatgcac agcaagaata tcactgatat attcttcaat atattgacta tgtcgaagac
     1681 ttaagtcgct gataatcagt atttttttat cagaagtata atggtgaatt tcaggattac
     1741 cgataagcat ccaaaagata tagctagata aataataaat cagattctga ttcagtagag
     1801 tggaataaaa ttgaagttct gcacgaaatc cagcatatag acgtatctgg cggtgttgca
     1861 tctgctgtgc aaagtaagtt gcacggatcg tcaaatgagt cgtatcgtat gggaaaatac
     1921 gggagaaaaa ataaatttgc gttaataatt gaacaacata ttcttcgttt ttttctttgc
     1981 aatcagcgga tgaaaacgaa cagtaggaca tattttttcg tatctgctca ctgatttcat
     2041 ttttttcttt ctccgaaaat tctgaaaaaa tcgtattgac aaaatagttc actactttta
     2101 tatagctgct gtacttcaat tttcctaaga gatttttcaa gattaagaag tcttttttat
     2161 cgatatttat caactgtaca taagagtctc cagctttcca ttcaaatgga gttccttggg
     2221 attctctcat gagatgcaca acaaaataca taataaagaa agattgctca tatgggcttt
     2281 cttcctgaaa caaattcatc tgacaattat tcttgaccat aatttctttg atcttctttt
     2341 tttcgattgg aaatggcaac gtgcgtcttc cgtagtaact taaaaagaaa aaggtaacaa
     2401 agatacgcaa ctggagctca cttttaaggc tagttaactg aaaaccttgg tcaacgatca
     2461 cttttaataa atagctttct aaaatttgat tgcattgttt caacttacgt gtaaaagaag
     2521 accggctgat ggaaattttt tcacaataat actcaatcgt tttacccgga ttgacaataa
     2581 tctcatttaa taaacgactg tttgtagaac tttgcgacat ttccatcaga acatttaaat
     2641 aagctggtac attcaaatgt tcaagacgat accccaattt ttgtgaagat tgaatcgtta
     2701 catccgttcc ccagcggatt ttgatctctt cgatatacct tacaaatgta gattctgaac
     2761 taccaattcg tttggcaagt tcttttttag tcatccactt gtcagaaatg gacagaattc
     2821 gaatgatttt ttccatcttt ctatagtttc gttccaacaa cctgatcata tgtttttctc
     2881 cctcaaccct tttctttcaa tgaaacgctc attaatcgaa ggaatcaact tcataaatga
     2941 aaccaattgg tatgaaaatt ggtgtgattc cctttagcgc ccttcgtata ataattatca
     3001 tattaaaata aataaaatta tgtaacgcct gtttatttta ataattttca ctattcacgt
     3061 caaaacagaa aaaaagaaaa aggaggggaa aaatgacgac cacagggaag aaactgaaag
     3121 ttattttcat gctgataata ttgagtttat caaactttgt gccattatct gcaatagcag
     3181 acactacaga tgatccaaca gttttagaaa caatttcagc tgaagtcatt tcggatcagt
     3241 ctggaaaaaa agcactgaac atcaagctaa atgcgaataa caccagtgct gaaaagatag
     3301 aaaaagaaat tggtctagtc gaaaattact taagtgatgt ggaaagaaaa gaaggagatg
     3361 gctatgctta tcaggtaaat agcgggaaaa ttacgttgga aatctcatca aacactaaac
     3421 aaactatcga tctgagtttt ccaatcgatc cagcacttta ccacagccag gcaaacaagc
     3481 tgatcgtcga taataaagaa tatgacatta ttgatgagac agaaaataag aaagatacag
     3541 atgtgtcagt accaaagcca gacgaaatag aagaagaatc atcaaaagaa aacgaaaatt
     3601 ctgtcagccc atttacattg cctacattat ccttgccagc tgtgagtgtg ccatctaatc
     3661 aaacgattcc tacagaatat acaacagatg atcagggcac ttatcctaaa gccagttggc
     3721 aacctacagg aaatacaaat gttcttgatc atcaaggcaa taaaaacgga acaaatcaat
     3781 gggatggtat aaattcttgg aatggagatc ctaatgatcg gacccattcg tatatcgaat
     3841 atggaggaac cggtaatcaa gcagactatg cgatacgaaa gtatgcaaag gaaacaagta
     3901 cacccggatt gtttgatgtt tatttgaatg ctcgtggaaa tgtacaaaaa gatatcacgc
     3961 ctcttgatct cgtattggtc gtagactggt caggaagtat gaacgacaat aatcggatcg
     4021 gtgaagtaaa gattggtgtc gatcgttttg tcgatacttt agcagatagc ggtatcacag
     4081 acaaaatcaa tatgggatat gtcggctact caagcgaagg atatagctac agtaacggtg
     4141 cagtacagat gggttcattt gattcagtga aaaatcaagt aaaatccatt acaccttcac
     4201 ggacaaatgg tggtactttt acacaaaaag cactaagaga tgcaggaagc atgctatccg
     4261 ttccaaatgg acataaaaaa gtgatcgttt tgctgacgga tggtgtacca acattttcct
     4321 ataaagtaca gcgggtacac gcacaatcaa gcagcaatta ttacggaact cagttttcta
     4381 atacgcaaga tcggccggga aatacttctc taatctcaag aatctatgat gcacctgacc
     4441 aaaacaatct atccagaaga atcgacagta cgtttatcgc aaccatcgga gaagcgatgg
     4501 cactcaaaga acgaggaatc gaaatacatg gtcttggcat ccaacttcaa agcgatccgg
     4561 cagctggtct ctcaaaagca gaagtagagt ctcgtatgcg acaaatggtt tcatcagatg
     4621 aaaaaggcga tctttactat gaatcagctg atcatgcaac agatatctct gaatacctag
     4681 ccaaaaaagc tgtacagatc tcagcaactg taagcaatgg acaaataaat gatccaatcg
     4741 cagaaccatt catttatcag cctggtacac tttcagtcaa gagtgtgggg acaagtccta
     4801 caacggtcac tccatctatt tccatagaag gaaataccat caagagcaat cagatctatt
     4861 taggaaaaga ccaagaaatc caaatccatt accaagtgag aatccaaaca gaaaatgagg
     4921 acttccatcc aaatttctgg tatcaaatga acggcaggac aactttccag ccaaacattg
     4981 ataccaatga attagctgaa ttcggtatac catctgctaa agctcccgga gtcagtcttc
     5041 acatcaaaaa gttatgggaa gaatttgaca acaatctagc tgatcgtcca gatcaagtta
     5101 cttttgagat tcaacgggaa catacgacaa atgctgcagc ttggaaaaac ggatatattc
     5161 gaatcattaa accagctaaa gatacaacaa atacgtggga acgtgcagac attgacaaat
     5221 tatctgcaaa tagcggagaa agttatcaag agatattatc actacctcaa tacaataatc
     5281 aaggtcaagc attcagttac caaacaatca aagaattacc tgtaccagga tacgattctc
     5341 aacaaataga tgcaatgaca tggaaaaata ctaaacaatt cacaccgtta aacttgaaaa
     5401 taacgaaaaa ttcctctaca ggtgaaaagg atcttattgg cgctgttttc aaattaacag
     5461 gagattctat tgatacttta ctaacagatc atggcgacgg aacctattct cttccagaaa
     5521 atgtcaaatt gcaaaaagaa atgacctata cgctgacaga aacaaaagct ccagaagggc
     5581 atggattaag caaaaagact acttgggaaa tcaagatcgc ttctgatggt acggtaacca
     5641 ttgatggaaa aacagtcact acttccgatg atacgatcca gttgactatt gaaaatcctt
     5701 ttgttgaagt tcctgtagca gtacgtaagt atgcgatgca agggacggac aaagagataa
     5761 atcttaaagg agcagcattt tccctacaga aaaaagaagc aaatggtact tatcagccaa
     5821 ttgacagcca aacaacgaat gaaaaaggtc ttgccagttt tgattcactc acacctggta
     5881 aatatcgagt cgttgaaaca gctggtcctg ccggatatga tacttcgccg ggaaattatg
     5941 aattccaaat cgataaatat ggaaaaatca tttacacggg aaaaaatacc gagatgacaa
     6001 ataatgtatg gacgctcact catcaaaatc gactaaaagc gtttgatcta acggtacaca
     6061 aaaaagaaga caacggacag acattaaaag gagcaaaatt cagactgcag ggaccagaaa
     6121 tggacttaga atcgccaaaa gatggacaag aaacagatac ctttctattc gaaaatttaa
     6181 aacctggaac ttatacgctg accgaaactt ttacaccaga aggataccaa ggtctaaaag
     6241 agccagttac tatagttata cacgaagatg ggtcaattca agtggatgga caagatcatg
     6301 aatctgttct gtcaccagga gccaaaaaca accagatttc tttagacatc acgaatcagg
     6361 caaaagtacc attacctgaa acgggaggaa ttggccgttt aggaatctat ctagtaggga
     6421 tgattggttg tgcgttttct atttggtatc tttttttgaa aaaagaaaga gggggcagct
     6481 aaatatgaaa aaacttggtt ggcttagtat gtgtctcttc ttgttactat ttaaaccagc
     6541 ttttactcag gtagcaacag aaacagaaac agaaatggtt cagattactt tacacaaatt
     6601 gcttttccca aacgggcaac tgccgaaaaa tcatccaaat gacggacaag aaaaagcttt
     6661 attacaaacg tatcgaggat taaatggtgt cacattccaa gtttatgatg tcacagattc
     6721 tttttaccat ctacgggaaa agggcaaaac ggtagaagaa gcacaagcag agatcgcaaa
     6781 aaacggtgcg tcttccggta tgtttaccgc agaagcaaca actacaactc ttaacaacga
     6841 agatggtatc gcttcttttt ctctggccgc taaagatcaa gaaaaaagag ataaagcgta
     6901 tcttttcatt gaatccaaag taccagaagt cgtcaaagaa aaggcagaga atatggtagt
     6961 tgttcttcct gtacatggac aaaacaatca aaaactttca actatccatt tgtatcctaa
     7021 aaatgaagaa aacgactacc ctgatccacc ttttgagaag gtattagaag agcctagaaa
     7081 tgattttacg attggtgaaa aaatcactta ttccttgcat acgacaattc ctgtaaatat
     7141 ccttgactat caaaagttcg aattgtcaga tagtgcggat gaagcattaa cgtttttacc
     7201 taatagttta acgatttcat cgaatggaga aaagctgaca gaaggctttg tcatacacaa
     7261 gaaacctcac ggatttgatg ttttattttc gatcccttcg ttggaaaaat atgctggaaa
     7321 aaaactgacc atttcttatc agatgcagct aagcagtaca gcacaggcga acaaggaaat
     7381 caacaacaac ggaacactgg attttggttt tggtgtcagt acaaagaaag tctctgtata
     7441 tacagggagt aagcaatttg tcaaaatcga gacaaataaa ccagataaac gattagctgg
     7501 cgcagtattc cttattaaaa acaaagcagg aaattacctc cagcaaacag ccaacggata
     7561 caagtggaca aagaacgaat cagatgcgct tcacctgatt tccgataaaa atggcgcttt
     7621 ttcaatttcc gggttgaaaa caggaagtta tcgattaaaa gagatcgaag caccttctgg
     7681 ttatatttta agtgaaacag aaattccgtt taccatttca acttttcttt ctgaggataa
     7741 agaggcggac agtatattga aagtagtcaa taaaaaagaa aatagccgtc catttcttcc
     7801 aaaaacaaac gaaacgaaaa atacactttt aggcgttgtt ggtatggtat tcgcaagctt
     7861 tgcaatctgg ttgtttatca aaaaaagaac aggagtgaaa aaatgaaaaa tcataaaaaa
     7921 ataaacgtta tgttaggagt ccttttcctt attttaccat tactcacaaa cagcttcggc
     7981 gcaaaaaaag tgtttgcaga ggagacagca gctcaagtca tccttcataa aaagaaaatg
     8041 actgatttac ccgatccttt aatccaaaac agcgggaaag aaatgagcga attcgatcaa
     8101 taccaaggat tagccgatat ttcattttca gtttataacg tcactcaaga attttatgcg
     8161 caacgagata aaggagcgtc cgtggatgca gcaaaacaag cagtccagtc tttgactcct
     8221 ggtacaccag ttgcttcagg aacgacagat gctgatggaa atgtcacttt atctttacct
     8281 aaaaaacaaa atgggaaaga tgcagtctac acgatcaaag aagaaccaaa agacggagtg
     8341 tcagctgccg caaacatggt tttagctttc cctgtatatg agatgatcaa acaagcagat
     8401 ggctcttata aatacgggac agaagaacta gatactatcc atctctaccc taaaaataca
     8461 gtcggtaatg atggaacgtt gaaagttaca aaaatcggta ctgccgaaaa cgaagcacta
     8521 aatggagcag aatttattat ttctaaagaa gaaggaacac caagcgtcaa aaaatacatc
     8581 caaagtgtca cagatggatt gtacacttgg acaactgatc aaaccaaagc caaacatttc
     8641 attactggtc attcttatga catcggcaac aatgactttg ccgaggcatc tattgaaaaa
     8701 ggccagttga tcgttaatca tttagaagtt ggaaaatata atttagaaga agtaaaagct
     8761 cctgataatg cggaaatgat tgaaaagcaa acaatcacgc cttttgagat cctggcaaat
     8821 agccaaacac cagtagaaaa gaccatcaaa aatgatacgt ctaaagttga taaaacaaca
     8881 cctcaattga atggaaaaga tgtcgcaatc ggtgaaaaaa ttcaatatga gatttctgtc
     8941 aatatcccat taggtatcgc tgataaagaa ggaacgcaaa acaagtacac aacattcaaa
     9001 cttatcgata ctcatgacgc tgctttaaca tttgataatg attcttcagg aacgtatgct
     9061 tatgccttat atgatggaaa taaagaaatc gacccagtaa attattctgt cactgagcaa
     9121 acagacggat tcacggtttc agttgatccg aattatattc cttcattaac tcctggcggt
     9181 acattgaaat tcgtttacta tatgcatttg aacgaaaaag cagatccaac caaaggattt
     9241 tctaaccaag caaatgtcga taacgggcat acaaatgatc aaacaccacc gtcagtcgat
     9301 gtcgttactg ggggcaaacg atttgttaaa gtagatggtg acgttacatc agaccaaaca
     9361 cttgctggag cagaattcgt cgttcgtgat caagatagtg acacagcgaa atatttatcg
     9421 atcgacccat ccacaaaagc cgtcagctgg gtatcggcga aagaatcagc aacggttttt
     9481 acaaccacaa gtaacggttt aatcgatgtg acaggtctaa aatatggcac gtactatctg
     9541 gaagaaacga aagcgccaga aaaatatgtt ccattaacaa accgtgtagc atttactatc
     9601 gatgaacaat cttatgtaac agcaggacag ttgatttctc ctgaaaaaat accaaataaa
     9661 cacaaaggta cacttccttc aacaggcggt aagggaatct atgtgtatat cggtgcagga
     9721 gtagtccttc tactgattgc tggactgtac tttgctagac gcaagcacag tcagatttag
     9781 tctttttgca agaattggaa aaaggagcag cggattacgc aaatgctcct ttttctttta
     9841 ttatcaaaca gtcatcacaa ggagaacaca aatgaaacca aagaaaagga aacttctaga
     9901 tctgctgatg cttttcttgc ttctctcagg aatagggata ctagcttatc catttgttag
     9961 cgacgcgctg aataattatc tggatcagca gatcatctct cattatcagc aacaagcggt
    10021 caaagaaaat gaagaagtca tggctaaaat ccaagaaaac atgacaaaga aaaaccagca
    10081 attagcaaaa gaaggtggaa atccaggtgc agatcccttt actaagaaaa aagaacctgc
    10141 gaaaaaggat agaacctatt ttgagaaaca tacgatcggt attttgacga tccctgagat
    10201 caatgtccat ttgccaattt ttgatgaaac gaatactgtg ctgctagaaa aaggtgcctc
    10261 attattggaa ggaacgtctt atcctacggg gggagaaaat acacatgccg ttatttccag
    10321 ccatcgagga ctgcctcaag caaagttgtt cacagactta ccgaaactga ctttaggtga
    10381 ttctttctat atcgaaatca atggacggac actcgcttat caagtcgatc agatcaagac
    10441 ggtagaaccg actgatacaa aagatctgag aatcgaaaaa gggcaggatt tcgtgacact
    10501 cctaacttgt acaccttata tggtcaacag tcatcgctta cttgtcagag ggcaccgggt
    10561 tccttatcat gcaaaagaag ttcaaaaaga agtacagaaa gtatccgaga acaaacgatt
    10621 tttcctgtat ggattgttag ctgcatgtat actgattttc ctccttctta tatatctttt
    10681 tcgtcaatcg ttcaaaataa gaaacagaag ttagctattc ctcatttcta taatatatct
    10741 attcgaatcg ttccgtaaac aaacaggctg aggtttgaaa aagaaaactt cagcctgttt
    10801 ttatgatgat atcaaagaga gcctatcaaa cactatcttt cttattacta aatgaggatc
    10861 ttttcttaag ttttccgata ttctttaaga aggccttttt gcatcaattc ttgtttgttt
    10921 ttcaagttgc ggatattctc ttcaaaattt tgagaatata tcactgagaa taacgtattt
    10981 aatacgtaat cgaaggcaat ttgagaggag aaagtcgcta ctttagcaaa atcatattcc
    11041 tcctgcgata taacaagaca catatcagcc tgtttggcca tttctgaatg ctgatttccg
    11101 gtaatcagca aggtaggggc ctctacatgc ttcaaatagg tcattattcg ttcataatga
    11161 tggatgcgac cgctgtagct aataaaaatg gcgcaatcct cgctagtcaa attggctgca
    11221 ttccaagaag aatcactgta ttcttcagcc atgatcaaaa acttattcaa cttgaccatt
    11281 ttattctgga atttccttgc cgtgatttga gaatcacctt ttgcaaaaag aaagattcgc
    11341 tgtgccttgt tcaatacttt accgatttga tctaaatctt catgttcaat ctgcaattgt
    11401 gcttttttta ttgactggat cgataaatcc gccattttct ttgcaatttg ctgagtgcta
    11461 tcttcacttg aaaaaggaaa attaggatca atcgtttgat tgaactgagc aatcaattga
    11521 tgaacttccc gccccagctc gaaacggaat tctttaaatc cgctgaatcc catttttttg
    11581 cataaacgaa tgatagctga atgggaagta tacgtagctt ttgctaattc ttcaatcgtc
    11641 atgtagactg cagcttcgat attcctacgg atgtaatcga caatccgttt ctctgtcgtg
    11701 gtaaacaact cttgttttgt tagtttttct tcaataagca tgatctgacc tccaaattct
    11761 atcttactga taaacacttg gttccgctac tatctttttg atttttatcc ttactttgaa
    11821 cattaatttc accaaaccga atgataacaa gtgtttttct gaactttttt accagaatag
    11881 ctattataaa tactttctgc gcttttctag gatatactga aaacatcaat aaaaggaggc
    11941 accactcatg cttacaatcg cttatcttgg caatggaaag agtacaaatc gctatcattt
    12001 gcctttttca agaaaactta cgaacaaaat caatatcaaa aaaatttttt ccccatctga
    12061 accagtgtgg aaaacattcg aagacatcga atatactgat cagctgtctg atctttggga
    12121 tgatcctgag atccaattag ttgtcatcac tacacctagt caatttcatt atgaatatgg
    12181 aaaaatggca ctcgaacatg gtaagaatgt actagtagag aaaccttttg cagaaacgtc
    12241 aaaggaagca gaagagttat ttgcattagc aaaagaaaaa ggtctattta tccaatgtta
    12301 ccaaaacaga cggttcgatt ctgacttctt gacagtccaa aaagtaatcg aaagcggaaa
    12361 attaggggat attttagaag tagaaatgca ttatgattat ttcagaccgg aagtaccaga
    12421 aaatacgcat gaattttcta ctgcgaacag ctatttatat ggacatgctt gtcatacagt
    12481 agatcaagtt ctttcttatt tcggcgaacc agatgatatc agctatgatg tacgtcaact
    12541 tttagggaaa ggacgaatga atgattactt cgatctggat tttttctatg gaaatatgaa
    12601 aatttcgata aaatccagtt atttccgcat caaagaacgt ccaagtttcg tcgtttacgg
    12661 aaaaaaaggg atgttcgtga aacaaacaaa agatcgacaa gaagaacatc tgaaaatgtt
    12721 ttacatgcct gatcatgaag actttggtat cgatttgccc gaacattatg gtgtattgac
    12781 ctatatagat gaacaaggga gttatcatga agaaaaagta atttccgagg ttggtgatta
    12841 cagccgtgtc tacgaaggga tttacgagac attagttaat ggcgcagaaa aagtcatcaa
    12901 agatgaagaa acaatcttac aaatgaaaat tttggaagaa ggaatcaaaa gcattcaaga
    12961 atcagaagga aaaatatgac atatttgtaa gaatcgggtt tgttttttga cgcataaact
    13021 gtcttattgt acgatgaaaa agtatagaac tactaggagg aaccaacatg acaaattatg
    13081 acggtgaaaa acacaaacga ttaagaggtt caatggcccc taaaacagaa gaagaagtgg
    13141 ctttagcagg aactaattct ggtgaaacag aagacaaaga agaatcatta gctggcacaa
    13201 actctggtga aacagaaaat aaagaaacag ctttatctgg ttcaaattct gtagaagagg
    13261 aaacaaagcc taatgagccc aatcactttg aaaaagatat cgaaaaagcg gttgaagatg
    13321 tcaaagaaaa atttgaagaa ctgacctcaa aaaataaaca tgatcatcat tgatcggacg
    13381 aaatcaattc ataaaagcag gagacatttg gtcacctgct ttttttctgt atccattcga
    13441 tagaaatcgt tcggtacagt cgtaaaaaaa atccaactgt aagttgtacc aaacacccct
    13501 cgatttaaaa gcgctaccat actagaatat aagtaggagg tgaacagcgc tgtgaactac
    13561 aaaattagtt atctcctgaa aacaccaaga gaaatcagtc tagaaaaagc agcagagatc
    13621 atcgaagtaa atacaagcga ggcgatcaaa caattaactc cttttgccga atatgtggat
    13681 gatacccgta tccgtataaa gaaattccga gcaatcgact gggcagaaaa atttttggat
    13741 attactcgat ttgaagctgt gtataccgaa caagagcgaa gagatcttct ttatttgatg
    13801 gtttattcga ctgcaacgtc tgtttctgtt tttcattttc aagattttct tcaggtttct
    13861 aaagggacag tcctgtcaga tatcaaaaag ataagaaatg aattagaagt ggaagattgt
    13921 cagctgtcgt atactaggaa aactggtttt aggctgctag ggtcagaagc aatattaaga
    13981 agagaagcaa aaaatcgcac tgctcgtttg atgcagtcac ttgctggaag atttgggctc
    14041 acgtatttaa ccgcaaccct ttccattgat tattacgcca aagttagaga tttgctagat
    14101 aaaacgattt cactatcaga tcttcgattc gcaccaaatc gaatggatga attagcttat
    14161 tatttgagtt tgacaaccat tcgaattgga aaaacatcca tggaactggt aaaagatgct
    14221 gaactgttgg cttctcttgc agtcactgac attagtcgac aagtattgac tggccttggc
    14281 tattccgaac atcaaaattc ggagattata tttttctctc tttgcttagc atcgatcgtt
    14341 gaaggagatg tccaggagcc tgcacttgat tttttgctgg aatgttcttc agaagtcatc
    14401 catcgcatgg aattcctgat ggccgtagaa tttgcttcgt ttcgtgaatt attgatgaat
    14461 gtcttttatc acttggtccc tgcctatttt cgtatttcct attcttttta tttacctaat
    14521 gtgatgatcg atcaaattaa gcatcaatat gcttccatct acgaaatgac aagaaaagca
    14581 ttacttccgt tagaaagacg aatagggaag agcatccctg aagaagagat tggttttttc
    14641 actatactat ttggcggaga gatcagaaaa gcggacgcag aagaaagaaa tagaaaaata
    14701 agagccgtga tcgtctgtcc aagtggcatt agctcttcat tgatcttaaa aagtgaactt
    14761 cagcaattat tcccgatgat cttttttaca gagactaatt cagctgaccg cttacacgag
    14821 gttgacgaaa cttcttacga tattattttt tctactgtac ctctcacact tactgataag
    14881 aaaaagtctc tctatgttct gcatccaatc atgactgact tggaaaaaaa tcagctgatc
    14941 aatcaagtac aaagagattg gctgatacca ggattttcaa tgccttctgc ccaagaattg
    15001 ttgtcggcgc tactgcctta tatcgaatta aaagaaggcg tagacgaaga aaagctctat
    15061 caagtattaa atcgtaaaat gaacaaactt ttagaaaaga aggaggatca acgtcctatg
    15121 ctatctgaat tattaacaga agaaatgatc caattatcgg atcaaccaaa aaactggcaa
    15181 gaagcaatca catttgccgc acagccgcta ttatcagaaa ataaaataga agaaagttat
    15241 atcaaagcta tgatcgagcg cgtggagcag tatggtgcgt ttatccatat tggcgatcac
    15301 atcgcattac ctcatgctcg tccagaagat ggcgtaaacg aagtagggat gtctttgctg
    15361 aaacttcaac aacctgtgga tctgcttgat aatccagccc atcccattag tttgttcatc
    15421 tgtttagctg ctatcgataa tgaggcacat cttcgggctt tggcaaattt aacaaagcta
    15481 ctttctaata aacaaaatct tcaagattta ttacacgcat caacaaaaga agaaatatta
    15541 gcgatcataa gaaaaggaga cgaatgatat gaaaattttt gcagtatgtc aaagtggatt
    15601 aggaacaagt tttatggtag agatgaacat caaacaagca cttgcagatc ttggagcaga
    15661 tgtggacgag tttgaagtaa gtcatacgga tgttggaagt gtatctagcg atatggctga
    15721 ttatttcttc attgagcatt ctttgaaaga tacgctaact tctatacctg atgaaaagct
    15781 cgtacctttg caatcgatca ttgacagcga agaagtaaaa gaaaaagtaa cgaagatctt
    15841 gtcaaaagaa taaaaatgat tcaagggagg aactatcaat ggataaattg ttgaatttgt
    15901 ttgtgtcaat cgtcagtcag ccctccatcc tcgtttcttt gattgcactg cttggtctcg
    15961 ttcttcagaa gaagaaattt tcagaagtga tccaaggaac tatcaaaaca tttgtcggat
    16021 tcctagtact gatcggcggt gctggtttaa tcagtaattc actaacccct tttgcttcga
    16081 tgttccaaac agctctgcat actcaaggag tagttcctag caatgaagca gtagtggcga
    16141 ttgctttgca acaatacggt acacctactg ctttgatcat gctagtaggg atgatcgtga
    16201 atattttact tgctagattc acaagatata aattcatttt tcttacaggt caagcaatgc
    16261 tttatgtttc ttgtctaaca gcagtcattt tagtaagcgc ggggtttact gcaaatttgt
    16321 ggatgatctt attaggcggg gtcttcgaag gaacactatt aactattacg cctgctcttt
    16381 gtcaaccgtt tatgaagaac atcactggaa acaatcaagt tgcgttaggg cataccggaa
    16441 atatcggtta tgcttctagt ggatggatcg gaaagttttt tggaaacaaa gaccgttcta
    16501 cagaggacat aaagattcct aaaagtttca gctttttgcg agattctact gtatcgatca
    16561 tgctcttgat gtcgatcgta tatgtcgcac ttgctttgtt agctggtcct ggatttgttg
    16621 aaagagaact aagcaatgga gacaacgctg taatctttgc actgatccaa gctggaacat
    16681 tcacagctgg gtttgttatc gtccttcaag gggttcgaat ggtgttggca gaaatcgttc
    16741 cagcattcca agggattgct aaaaaactcg tacccgattc taaaccagca ttagatgttc
    16801 ctatcgtttt tccatatgca cctaatgctg tattaattgg cttttttgtt agcttctttg
    16861 taggaacact atccatgttt gctatgatag gcttgcaaac tacagttatc attcctgggg
    16921 tagtaggaca tttcttctgc ggagctgctt ctggtgtctt cggcaactcg actggcggta
    16981 gaagaggggc ggtattaggt tctgctttca atagcttatt gattagttgg ctgccattat
    17041 ttatccttcc agtattagga gatcttaaaa tcgcttcttc tacatttgcc gatactgatt
    17101 atctagtacc aggacttgta cttgggaaac taggagaata cggtcaaata gccttgacag
    17161 ttggaatcat cgctttcttt ttacttgtcg tattatctag ctttcttttt gccggaaaaa
    17221 gaacaaacat tgtttctaaa gaacagaatt aagcaaactt caaaataaaa attgcttgga
    17281 caatatcaga agaagattta aagtcttctg atatgtcgca agcagttttt tgttttatgc
    17341 atttattttt gaatagcttg ttaagacaag tggctgtttt gctagaagag gataactttt
    17401 accattcatt ttcactgttc cgcctgttct atcagaagta atcacgatct tatcttttgt
    17461 tttttcaagc gaaatctttc catctttgat aggtagcgta caattcagtg tttcaaatga
    17521 atctagatta ggttgtatca taaattcttc ataacctggc tttgttggat acaaaccgat
    17581 aaaatagcgt cctaaaagat agattgggct tgctccccat gcatggcata agctttttcc
    17641 ataaggatct ccatacatac tgtacttttc atttcctgtt tgagtagggt cgtattcttc
    17701 ccagaaagtt accgcatcat gagataacat ccctccccaa taatcaagaa tcgtttggta
    17761 gacgatattc gtctgtccga gtttgcataa agcatcttgt tcatagaatt tgaaatatgg
    17821 agtagtaatc tgtgtgatag tgtcattcaa taagacattc tttaagatcg attgctgttg
    17881 gtctttgtta gcaaaatcga ataaaacagc gaaaatattc gcatgacgcg tcacatttct
    17941 tctcccagat tcatagcaat cgataaaagc gccaagttct tcatcccaaa agtagtcaaa
    18001 taggttttgt ttcaatagtt gatatttttc ttgatagaga tgagtatgat aattttcgtt
    18061 ctctccatat gtttcagcta agatatctcc gcaagtgatc atggaatgat aggctttcag
    18121 taataagact tgttctgcag caagcgttcc ttctttatcc atttcagacc aatcaataaa
    18181 tatccaatct tttgctctcc cataaataaa ccctaattca ttcgtttgct ccatcaagta
    18241 tctcattaat gattgcatct tagggtagac gatgcgtaaa aattcatagt ctttcgtcat
    18301 catatagtgg ttttcgatac caatcaacca caagatcgaa tagtcaacga tagtattcat
    18361 atgctgcttg atttcatttt gtcctcttaa tgctaatatc gttcgtttat tgatttcctc
    18421 gtcaaaaaaa agatattgat tgatcatgaa tgattgatac gcatctccag accaaatcca
    18481 gcgatctcgt ttgataccgt cgataaaaaa caaatcacta cataacttga aggcttcttc
    18541 tgatactgac cagatctgat tcagtaaaga agaatcgctt gaaaaagcag cttgtttcga
    18601 aagagataaa aattcatgaa aaacttgaag ttgaaccatt ccagtagaaa cctctggtat
    18661 aaacaaatag cgaaaagcgc gtttacgagc tttcattttt cttgtcattc ctatttgctt
    18721 atagtagcaa taatcgatat ccatagcttc tacatcggac tctccataac atagagtgat
    18781 agattcgata tggtcatcca gagatattag ttctacacag ccattcactg ctcgcccaaa
    18841 atcgaataag attcctccgc caatctccgt ttcagtgact ggatggacgc attctttttg
    18901 ataatagatt tgatttggat cttttgcgat atcgacataa agcggactcc agccagctgg
    18961 atattcttca ataaaattac ttgctgtcca gccaatatct gattttactt cgtctccctc
    19021 gataaacatc gctggtagcc ctgatgtatg tcctacaaag atacgaattt ttgctttacc
    19081 agctggacaa gtcagccatt ttccacatgg atgtttttgt ccattaattt ctacataccc
    19141 aactccttgg atcgtcactt taaacatgga aggttgatca agaaagtaat atctcttaaa
    19201 cttgacgttc cgatggcaat catccatgta ccagtacgct ggccagtcaa aacctcgttc
    19261 ttcccgttgg aaattttgta gcagtccatg gcggatctca aagtctccag gataccaaag
    19321 ccatctatct ttatgttttt catttgtcat tttacacgac ctccatcaaa tttattttgt
    19381 tgtcaattgt tcttttattt cttcatctac tcttatcact gtctttctat tcgtcctgtg
    19441 tactactaga aggctaataa gcgcgctaat caacagcaag atactgccat tgacgaaaac
    19501 agcaagtgtt ttactcccaa gagaagcaaa agagagaaag agcaaaccat atggtgcaat
    19561 gaaagtacct aaattagcgc caaccaataa gattgaagta ttgaaactct ccgcgtactt
    19621 gggcgcttgg atacttactt ggttgaataa gtaaggaatg aataacgaaa aagaaattcc
    19681 tgttaggatc gcaccaaacc caatcacata caggtttatc acagtggcaa tcaatacaaa
    19741 gccgattccc atcagtagta aagaaagtgt aagcatatag cgatgtgctt ttgtctgaag
    19801 ccagccaaaa caaactccgg cagccatact tgccaattgc atgattgtta agatattcgc
    19861 tgcattagtt tcgtttccta gagaataagt cgtcaggact gtggctaatt taatcgatac
    19921 agcattatat accacgatga ccagcaagag aaaaatacca taactaatca ttttaggatt
    19981 cgttttgatt acttgtcttg ttggggctgt gccatgcgat tgaagttcag ttgtttcagg
    20041 tgtaggaaca aacaaaacaa acaagacaag aataggtaag ctgatggcgt agactaaaaa
    20101 ggtatattgc caaccgaact gtactagctg tcctgcaaca aatgtcatta aagctgcacc
    20161 aaatccttga aatgcacttt gaaaaccaat catagaggct cgttcatctc cgtgataaaa
    20221 ggcagcaatc atgctgactg ccaaagaatt gatcaatcca aagccaatac ctagtccagc
    20281 tcttgaagct aagacaatac tgtaactttg tgagaagagg gggaggatac cacaaatcga
    20341 tgcaatcact aatccgatga tcacagtttg tttttgtccg attttcctag caataaaact
    20401 actaatcaaa acaaaaatag tgaccataaa agagggagca gtcgttaata attcaacagc
    20461 agataaagaa cgcgtggcat aagtagctga catactggga attgcagacg tgactgcacc
    20521 agcagaagtc aaaataagag agactgacag cagagaaaat ttcatcatct ttttattcat
    20581 aagcatgttc ctttcatttt tgtaagcgct tatcaaaaat atagtatgaa gtggttattc
    20641 tctcaatagc ggaatcacag gaagttcatg tcccaaccat agaaagtctt tatgaaacca
    20701 aaaatagctt tgcatataat agcagtacag cgtgaactaa taagcgaaag aaaccgctta
    20761 caaaaaggag ggagtcaatt ggaactagct gaattagaca ttttttttgc gtaacgaaga
    20821 atcaatcgaa actctgcagc gtgaacaagg aatgaatatc aatgatttaa acttagctta
    20881 ccatcttcag acaccgccaa aaatccaccg aggggatcta tttcaacagg gaaatattca
    20941 tatcagtaaa caccgtcgat atgcggaagt tcccgagcat acacatgatt ttgttgaaat
    21001 caattatatg tatagcggta catgtttaca aattattaat ggagaaagga tagagatgga
    21061 agaaagtgac gtggtgatca ttgataaaga agctactcaa agaatcgaat acacagggca
    21121 agaggatatt ttaatcaaca tcttattaaa agaagaaacc ttatctacag agatattgaa
    21181 tcatttggca agcacaaata atctactttc ttcttttttg ataaatgcat caaaagaaaa
    21241 aggggaacat acacaattca ttcattttcg cggcggaaaa acaagtcgtt tgacgcattt
    21301 gattgaaaat atgctgttga tttattttga tacatacagt cataagatcc gttcgttaaa
    21361 tctctataca tcactattac tgatcgaatt gactcattta ttagaacaac aaagcttttc
    21421 atcagcagat ggatgcgcag aaaatgagat gcttagagta ttagattata tcgataagca
    21481 ttttacttct tgtacgttaa aagaaacagc agctcatttt ggctacaatc cggtctatct
    21541 tagtaataaa ttgaaaatgt attttgatgc ttcctttcaa gaactggttc taaagaaaaa
    21601 attcgacttt gcgttggaac tgattcaaga aacgagtttt tcgttggaag aaatcgctaa
    21661 tcgtatcgga tataaacaaa cgacttcttt attcaaatta ttcaagaagt attcaacatg
    21721 tactcccaat gaatggagaa ataaacatta agaagaaaat ctttcactaa agcatatgaa
    21781 ggaaaattgt tttaggtgcg attgctgtct tattatatcg ttgcagcgaa tttttttgga
    21841 tttattttca gttctaaact tttggttgta ttatcagggg atataggtgt aaacatttgt
    21901 catttatctg ttatgcgtat tcaggaattc gtttccatca aaaattataa taacaactat
    21961 tataattaac atgttgataa aatgaataaa caagactata cttttcatga aagtaacaaa
    22021 tagttatgaa aaggagctgt ttattcatga aaaaaacaag tcgtaaagta gtaatcgtcg
    22081 gtacaggttt tgttggaaca agtatcgctt atgcaatgat caaccaagga atctctaatg
    22141 aattagtatt gatcgatgtg aatcaagaaa aagcagaagg ggaagcattg gaccttttag
    22201 atggtatggc ttggggcgat gaaaatgtgg ctgtatggtc aggtggctat gaagaatgta
    22261 aagatgctga tatcgttgtt atcacagctg gtatcaacca aaaacctggt caatctcgtt
    22321 tagatttagt taaaacaaat gcgtcaatca tgcgccaaat cgtaaaagaa atcatgggat
    22381 caggattcga cggtattatc gttgttgctt caaacccagt agatattttg acatatatcg
    22441 cttggaacga atctggtctg ccaacatcac gtgttatcgg tactggaaca acattggata
    22501 caactcgttt ccgtaaagaa atcgcattga aattaaaagt tgacccacgc agcgtccacg
    22561 gctatatctt aggtgaacat ggtgattcag aagttgctgc atggtcacat acaactgtcg
    22621 gtggtaaacc agtgtttgaa attgtagaaa aagaccatcg tattgcccaa gatgaattag
    22681 atgtgattgc tgataaagtc cgtaatgctg cttatgagat cattgatcgt aaaaaagcaa
    22741 cttactatgg tattggtatg agtactgcac gtatcgtaaa agctatcttg aataacgaac
    22801 aagctgtatt gcctgtatct gcttatttaa ctggtgaata cgacgaaaaa gatatcttta
    22861 caggtgtgcc atcaatcgtt gacgaaaatg gtgttcgtga agttgttgaa ttatcaatca
    22921 acgaagaaga aaaagcaatg ttcagcaaat caacaagtgc tttaagagaa gtattgaaca
    22981 ctgtattata agaaacaatg aatatgaact aaaaatccta aaactgctta acagctgaaa
    23041 tagctgttaa gcagtttttt attgatatct aataggtaag caaataaaca gaatggttaa
    23101 aattaatcgg tccgaaaatg tatccgtaca tatgaacata tgaaatttaa tctgttgatt
    23161 acaataagcg aatcgttatc aatgtttatc tgtggttaat taaaaaatcc ttccgcaacg
    23221 gcaaatacaa tgattcatat ctaattcgtt catatttgat taactgaata aagaaactgg
    23281 tttttatcat tcaattaaac gacaaaaaaa gtaagaaaaa ctgcaaaatt tttttataat
    23341 tttgcataag cagattttga tggaaaaatg acgatttttg ataaaaaacc gttcgagaga
    23401 ggtttatcat tttttaaatc tactttgtat aa
//
LOCUS       DACEZN010000025        23201 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEZN010000025 DACEZN010000000
VERSION     DACEZN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.025"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(161..445)
                     /locus_tag="IXH60_000779"
     CDS             complement(161..445)
                     /locus_tag="IXH60_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989741.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="IXH60_000780"
     CDS             complement(463..1353)
                     /locus_tag="IXH60_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1989742.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="IXH60_000781"
     CDS             complement(1373..1591)
                     /locus_tag="IXH60_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989743.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="IXH60_000782"
     CDS             complement(1561..3261)
                     /locus_tag="IXH60_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1989744.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="IXH60_000783"
     CDS             3528..4301
                     /locus_tag="IXH60_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989745.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IXH60_000784"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IXH60_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1989746.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="IXH60_000785"
     CDS             complement(5125..6096)
                     /locus_tag="IXH60_000785"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1989747.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IXH60_000786"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IXH60_000786"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1989748.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IXH60_000787"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IXH60_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1989749.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="IXH60_000788"
     CDS             complement(9051..9875)
                     /locus_tag="IXH60_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1989750.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="IXH60_000789"
     CDS             complement(9848..10528)
                     /locus_tag="IXH60_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989751.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="IXH60_000790"
     CDS             complement(10551..11489)
                     /locus_tag="IXH60_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1989752.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /locus_tag="IXH60_000791"
     CDS             complement(11734..12585)
                     /locus_tag="IXH60_000791"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1989753.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IXH60_000792"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IXH60_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1989754.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="IXH60_000793"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="IXH60_000793"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1989755.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IXH60_000794"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IXH60_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1989756.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IXH60_000795"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IXH60_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1989757.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="IXH60_000796"
     CDS             complement(19463..20704)
                     /locus_tag="IXH60_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1989758.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IXH60_000797"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IXH60_000797"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1989759.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22615)
                     /locus_tag="IXH60_000798"
     CDS             complement(21731..22615)
                     /locus_tag="IXH60_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1989760.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         6602 a         5050 c         4015 g         7534 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact t
//
LOCUS       DACEZN010000026        22857 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEZN010000026 DACEZN010000000
VERSION     DACEZN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.026"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(104..1336)
                     /locus_tag="IXH60_000799"
     CDS             complement(104..1336)
                     /locus_tag="IXH60_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1989761.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="IXH60_000800"
     CDS             1591..2160
                     /locus_tag="IXH60_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1989762.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="IXH60_000801"
     CDS             complement(2338..2778)
                     /locus_tag="IXH60_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1989763.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="IXH60_000802"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="IXH60_000802"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1989764.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="IXH60_000803"
     CDS             3732..4655
                     /locus_tag="IXH60_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1989765.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="IXH60_000804"
     CDS             4731..5870
                     /locus_tag="IXH60_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1989766.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="IXH60_000805"
     CDS             5863..6663
                     /locus_tag="IXH60_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1989767.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="IXH60_000806"
     CDS             6663..7490
                     /locus_tag="IXH60_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1989768.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="IXH60_000807"
     CDS             7468..8202
                     /locus_tag="IXH60_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1989769.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="IXH60_000808"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="IXH60_000808"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1989770.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="IXH60_000809"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="IXH60_000809"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1989771.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="IXH60_000810"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="IXH60_000810"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1989772.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="IXH60_000811"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="IXH60_000811"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1989773.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="IXH60_000812"
     CDS             11787..13820
                     /locus_tag="IXH60_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989774.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="IXH60_000813"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="IXH60_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="IXH60_000814"
     CDS             15353..16159
                     /locus_tag="IXH60_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989775.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="IXH60_000815"
     CDS             16171..17391
                     /locus_tag="IXH60_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989776.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="IXH60_000816"
     CDS             17381..18967
                     /locus_tag="IXH60_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1989777.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="IXH60_000817"
     CDS             19006..21144
                     /locus_tag="IXH60_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1989778.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="IXH60_000818"
     CDS             complement(21512..22495)
                     /locus_tag="IXH60_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ1989779.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7176 a         3899 c         4712 g         7070 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtg
//
LOCUS       DACEZN010000027        22743 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEZN010000027 DACEZN010000000
VERSION     DACEZN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.027"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            493..1200
                     /locus_tag="IXH60_000819"
     CDS             493..1200
                     /locus_tag="IXH60_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1989780.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="IXH60_000820"
     CDS             complement(1259..1903)
                     /locus_tag="IXH60_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1989781.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="IXH60_000821"
     CDS             2030..2317
                     /locus_tag="IXH60_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989782.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="IXH60_000822"
     CDS             2425..2745
                     /locus_tag="IXH60_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1989783.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="IXH60_000823"
     CDS             2826..4139
                     /locus_tag="IXH60_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1989784.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="IXH60_000824"
     CDS             4136..4855
                     /locus_tag="IXH60_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1989785.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="IXH60_000825"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="IXH60_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1989786.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="IXH60_000826"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="IXH60_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1989787.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="IXH60_000827"
     CDS             join(8416..8478,8480..9517)
                     /gene="prfB"
                     /locus_tag="IXH60_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1989788.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="IXH60_000828"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="IXH60_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1989789.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="IXH60_000829"
     CDS             10292..11185
                     /locus_tag="IXH60_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989790.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="IXH60_000830"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="IXH60_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1989791.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="IXH60_000831"
     CDS             13122..13979
                     /locus_tag="IXH60_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1989792.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="IXH60_000832"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="IXH60_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1989793.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="IXH60_000833"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="IXH60_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1989794.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="IXH60_000834"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="IXH60_000834"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989795.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="IXH60_000835"
     CDS             16735..17493
                     /locus_tag="IXH60_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989796.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="IXH60_000836"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="IXH60_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1989797.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="IXH60_000837"
     CDS             18406..19437
                     /locus_tag="IXH60_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1989798.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="IXH60_000838"
     CDS             19434..20201
                     /locus_tag="IXH60_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1989799.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="IXH60_000839"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="IXH60_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1989800.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22048..22353
                     /locus_tag="IXH60_000840"
     CDS             22048..22353
                     /locus_tag="IXH60_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1989801.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            22353..22703
                     /locus_tag="IXH60_000841"
     CDS             22353..22703
                     /locus_tag="IXH60_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1989802.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         7463 a         3921 c         4908 g         6451 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22021 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat
    22081 gttgttttga caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt
    22141 atccgtgtgc tttatgtatt cctaagctta gttgcttttg gaagtccaat cattttatac
    22201 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22261 aatgcatatt atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa
    22321 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta
    22381 atacgttaac attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta
    22441 tttggacagc cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga
    22501 ttctgacgat cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
    22561 tcaatgcagc gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt
    22621 cttcatttgg tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaatcgtta
    22681 gtgaacataa cagcagaaag taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt
    22741 tta
//
LOCUS       DACEZN010000028        22720 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEZN010000028 DACEZN010000000
VERSION     DACEZN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.028"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            186..1304
                     /locus_tag="IXH60_000842"
     CDS             186..1304
                     /locus_tag="IXH60_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989803.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1605..1925
                     /locus_tag="IXH60_000843"
     CDS             1605..1925
                     /locus_tag="IXH60_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989804.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            2377..2577
                     /locus_tag="IXH60_000844"
     CDS             2377..2577
                     /locus_tag="IXH60_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1989805.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            2724..5513
                     /locus_tag="IXH60_000845"
     CDS             2724..5513
                     /locus_tag="IXH60_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1989806.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            5560..6087
                     /locus_tag="IXH60_000846"
     CDS             5560..6087
                     /locus_tag="IXH60_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1989807.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            6108..7298
                     /locus_tag="IXH60_000847"
     CDS             6108..7298
                     /locus_tag="IXH60_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1989808.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            7338..8636
                     /gene="asnS"
                     /locus_tag="IXH60_000848"
     CDS             7338..8636
                     /gene="asnS"
                     /locus_tag="IXH60_000848"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1989809.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            9431..10105
                     /locus_tag="IXH60_000849"
     CDS             9431..10105
                     /locus_tag="IXH60_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1989810.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            10102..10443
                     /locus_tag="IXH60_000850"
     CDS             10102..10443
                     /locus_tag="IXH60_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989811.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            10473..10832
                     /locus_tag="IXH60_000851"
     CDS             10473..10832
                     /locus_tag="IXH60_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1989812.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            11026..11214
                     /locus_tag="IXH60_000852"
                     /pseudo
     CDS             11026..11214
                     /locus_tag="IXH60_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            11356..13440
                     /gene="cadA"
                     /locus_tag="IXH60_000853"
     CDS             11356..13440
                     /gene="cadA"
                     /locus_tag="IXH60_000853"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1989813.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            13751..15217
                     /locus_tag="IXH60_000854"
     CDS             13751..15217
                     /locus_tag="IXH60_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1989814.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            15527..16000
                     /locus_tag="IXH60_000855"
     CDS             15527..16000
                     /locus_tag="IXH60_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAQ1989815.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            16074..16514
                     /locus_tag="IXH60_000856"
     CDS             16074..16514
                     /locus_tag="IXH60_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1989816.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            16527..16736
                     /gene="copZ"
                     /locus_tag="IXH60_000857"
     CDS             16527..16736
                     /gene="copZ"
                     /locus_tag="IXH60_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1989817.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            16736..18922
                     /locus_tag="IXH60_000858"
     CDS             16736..18922
                     /locus_tag="IXH60_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1989818.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18942..21113
                     /gene="copB"
                     /locus_tag="IXH60_000859"
     CDS             18942..21113
                     /gene="copB"
                     /locus_tag="IXH60_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1989819.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(21225..>22720)
                     /locus_tag="IXH60_000860"
     CDS             complement(21225..>22720)
                     /locus_tag="IXH60_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAQ1989820.1"
                     /translation="DEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQ
                     KGNGYWKIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQA
                     FLPYPYGNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKS
                     SQLKADDLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRY
                     SKTDTGHTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTG
                     TDNQGNEEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAI
                     EQPNESENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLR
                     WTSYLIYLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRL
                     TTQYEAEIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
BASE COUNT         7517 a         3940 c         4902 g         6361 t
ORIGIN      
        1 aagaatagtt gaagactggg gctaaataat tgaggaaatt atgaagataa cttaaaaaaa
       61 tagctaaatt taaaaccgta gaaaccgttt tattttttta gatacgtttt attgtatgga
      121 aaaatagtgt tgtggtagac tttagataga aacaaaggaa tgattttttt aggaggggta
      181 cgtttatggc aaaaaaggat aaagaattat tgacacaatt gacttcagaa ggcgctaaag
      241 gaccattcgt cacgatcatg ctgaatacgc atgtcgccca tcaagatgtg gaaaaagatc
      301 agatcaaatt caaaaatttt gcgaaagaag caaagaaacg ttttgagaaa aaatattccg
      361 aacatgactg gtctccgttc caagaaaaaa ttgaaaattt gttaggagat ccttcgtttt
      421 ggcgtagtgc aactacgagt gtagcggtca ttataactgc tgaccagacc tatatccatc
      481 gtttgagtat cccggtagac gatcaatact atgtaagtga tcttccttat ttactggcta
      541 ttatcaaaaa tgggcagttc aactattctt attatttatt gggattgaac cgcgactcca
      601 tgaaattgta tgaggtcagc aacaaacaag taaaagaagt atctctacca gaaggggcac
      661 cggttgatgt cgttactgca ttgggatctg aactgacagg tggcggtttg aacttctctt
      721 ctaatggagg aggcggagcc cgagatggag taactttcca tggcgtaaat ccaaaagatg
      781 aagaagtaga gatcgattgg gtcaactatt accaagctgt tgatacgtac ttaaaggatt
      841 ctttcgaaaa tcctgaacaa tggccgattt atttatttgc attaccagaa aatcagacga
      901 tgttcaaaaa aattgctaaa aatccctatt attgtgcaga cgttgcgatt tctgcttctc
      961 ctgcgcagct atcctttaga gatatcgaag aaggggcgaa gaagctgtca agtgaactcg
     1021 cagctcaaga atcaaaagaa tacaataaac tcttagataa gaaattcatc gatcagttag
     1081 ttgatatcgt tccagctgcg aaagaaggaa aagtgtcaca tttatttatc gctacgtcaa
     1141 acttagctga tggtttcggt gaagatcctg aaacagaata tgatcggcgt caagtgttga
     1201 atgcattagc agatgatgtg atcattaatg gcgggaaagt gtttattttg gatcaaaaag
     1261 atgcgccaga tgaaaagagt ctgacagcga ttcttcgtta ttaaaagagg ttgtgaaatc
     1321 agtgttttga cctgagtatt aaggaagagg ttgtgacaga agtggtcagc ttcaagaaat
     1381 aagaagagat tgacaaaaat tatttctcaa atttttgtga aatttcggct tatttccgaa
     1441 gaagctactt ctggaacacc gtttaaaaaa gaggttgtga cgtggtcacg acctcttttt
     1501 gacgattatg atacaaaaaa catcatttat gaacaaaatg tgaataacgg gcgttcttga
     1561 gatatgattt taattgggaa aaacttatcg ataaggaggt ctttatgaaa aaaagactgt
     1621 tatggtgttt gctgtttcta tccctaagtt taacaagtca gccagttttt gctgaagaaa
     1681 cacagcggtc tcctattaca ggcacacgtc tgtacaaaaa tgtttatgta cagacaacag
     1741 atcttgattc tgcgattcct gaaacagaag aacggtcttt gcagccaaga tttggttact
     1801 tttggtactg tccgacctgt gattatgaaa gttgctggca tttcttacat tctacagctg
     1861 cagcttttgc ttttactcat actcagaaat atgatggaca tcatcctgtt atttatgagg
     1921 tatagatatg agcaaaaaag aaaaactaat ctcgttgatc gtattggttt gccttgttct
     1981 tctggtcact gtcataggct atcagaaata ccatgagaac atcagaccta ctaaagaatc
     2041 ttccccagcc tcgaccgttg aaacagaacc gataagtagt cagaaagaat aattttttta
     2101 gtttaaagaa gaacatcaga gcaaaaaaac accaactgaa caatatgtcg tttattttaa
     2161 aaaaagttcg ttttttcact ttatgtaatc gctgagaaaa ctttttttaa taaaaaaagt
     2221 cgaaaaactc tagctttttt gtaaacggta tgatatacta taggagtagt ttttgttcgg
     2281 taatctgtgg tagcttgttt caaataaaca tcttccagat atacaccatt caagtaattg
     2341 caaaatatgt gttgatacac aaggaggatt tttattatgg aaacaggtac agtaaaatgg
     2401 tttaactcag aaaaaggatt tggattcatc actgcagaaa acggaaacga tgtattcgtt
     2461 catttctcag ctatccaagg cgacggattc aaaactttag aagaaggcca agcagtaact
     2521 ttcgatgttg aagaaggcca acgcggacct caagcaacaa acgttactaa agcttaatta
     2581 acctatacta aaaaataaac gcccagacat ttgcgtgtct gggcgttttt gtctattttc
     2641 tttaaaaagc gatgagagta tttttctgca ctttgtagta taatttcaaa agaacattag
     2701 aggcaggaga agaggaagat attttgaaac caagaactta tgcagtcgtt gatatcgaaa
     2761 cgactgggac gaatccaaaa gaagatcgga tcatccaatt tggctgcgtc atgattgaat
     2821 caggtcggat cacttcacgt ttttcgatcg atattcatcc agggcggaag atttcaaaac
     2881 aaatccagca tttaacaggg atcacgaatc aacgcgtcca aaaagcgcct tatttcgaag
     2941 atgtcgccca aacgatttac aatcttttgg cagacaccat atttgtcgcg cataatattt
     3001 attttgatta taattttttg aaccatgaac tcatgcgatg cggacttcct agtttgaaga
     3061 taccaggcat cgatacagta gaacttgcac aaattttctt gccgacggaa ccaagctttc
     3121 gcttagctga tttgtcagaa agccttggat ttattcatga gaatcctcat caggcagata
     3181 gtgatgccga agtgacaggt caacttcttt tgctgatcga agaacggatg agaaaattgc
     3241 caatcatcac gttagagcag attgcgcgat taagtagaca cacaggtatg gatacgagtc
     3301 gtttcattta tcatgtgatt gaagaaatga aagaaaaacc agagccactt gattcctcat
     3361 tagaaatcgt cgatgggcta gctttgcaga aaaaagaagt ggaactattc acatccgtcc
     3421 attacggcga gcggacatat ccaagaaaaa aacaagcaaa agaaaagatg tttggtaaaa
     3481 cactgatgta tcgaaaagaa cagaacagac tgatgaatgc gatctacgac catttcacaa
     3541 aagatgaatc aaaagatttg atgatcgaag cagctacagg aatgggtaaa acaatcggtt
     3601 atctgttgcc attgagttat ttggctacac ctgaaaaacc cgctgtcatc agtaccgttt
     3661 cgctagtttt gcagcagcag atcatagaaa aagaaattcc attgctgaat caattgctag
     3721 atcagcccat acaacctgta attatcaaaa gttatcgcca ctatatcgac ctgcaacgat
     3781 ttaaaggaac acttgtggaa ccaccagaac aaaaacaata tgctttgtat caaatggcgg
     3841 tcttagtttg gctgacgcaa acgaagaccg gtgatttgga tgaattacat ttaacaaact
     3901 tgaaccatgc attctttgca gatatcgcgc acagaggtac tgggttttta gcaagaaatc
     3961 agtcgtttta cgaacaagac tttgttcgtt tcttgcaaaa gaaaatcagg caaagtaatt
     4021 ttttgattgt caatcatgcg tttttagtac aagaaacaca gcgaaaagaa ccattgcttc
     4081 cagctagtcc atttttgttg atcgatgaag tacatcattt gccggaaatc gctgaaaaag
     4141 taaatgacaa acgattaagt attaactatt tccataaaca aatccagcaa ttagaagaac
     4201 caaatcagct gtttgaccga atacaaagct atttaccgaa agaacatgaa gccttacgct
     4261 ggttatcgat ttatcgagaa gaactttctg cgctatgcga agcacagatt tgtatggcag
     4321 aaggattaat gagtttagtg agcggcaagc aacccaacgg acagtatcca gaagaagtca
     4381 tgatgacaaa agagatgctg gatcagctgc cactggaatc tgggacagcc attgatcacc
     4441 tgcagctttt ttatcaggag attcagcagc tacagtcaca aatgcgtctg acttttgaga
     4501 ttatccaaga aagctggaca aaccagatgc ggatcgattt agcaagttta tatagcttat
     4561 ttgatcaatt agacaagcag tacttgctga tggatcaatg gctggaagac tggcgagatc
     4621 atttgatcca ttggttcgtt cctgggaaca cagcgcagca gatgattttt catctccatg
     4681 atttccaagc ggctatgttg ccgtcgactg tttggtatcc tcgatatgat cgtattcttt
     4741 atattggagg aacgctgaag atcggcccta atcgacacta tctctctaag aggcttggca
     4801 ttgaagcagc acctcttaaa gtgattgcct ctccttatga ttatgagaaa caggcaagga
     4861 tctatgtgcc aaaagaggca cctgccgtcc atcaagtcag caatcaagca tacatcgatt
     4921 atttagagac agcacttaca ggtctgatcg aacaagaaga tcggccaata cttgtgttat
     4981 tcacttctca tgatgttttg cagaaggtct atcagaaaat gcatctgtct atgctagaaa
     5041 aaggacgaga aatactagct caagggtttg gtggaagccg ggagaagctt ttgaaacgct
     5101 tcattttgtc agaaaaaagc gtattgtttg gcgctgacag cttttgggaa ggtgtagatt
     5161 tgcctggaga tgctttgcag atcgttgtag tcacacgttt acccttcgat aatccgcttc
     5221 gcccgatggt caaagctagg aacgcttatt tagaatcaga ggggatcaat cctttttacc
     5281 aggaatccgt acctaaagca gctttgaagt tacgccaagc attaggtcgc ttgataagga
     5341 cggagacaga tcgtggcgta ttgcttattt tggatcgaag actagtcact gcaaaatatg
     5401 gcgcacggat catccatgca ttaccgaaaa aagtaccgat caaagagcta gctatggagg
     5461 aaatctatca ggatatccat gaattcttaa agaaaaatga agaaggaagt tagaggagct
     5521 gtcttttttc ataattaaaa tctgctataa tgaaaaggaa tgaagataaa ggagcgacga
     5581 cccttgaaga aaaaacagac aaaggaaaaa acgattaatc gcgtgttact tggcttgatc
     5641 gtatttttat tagcggtcat cgtgatcagc tcgatttttt acttgcgttc cagtcgtccg
     5701 gcggtgcagg cgaagaaaga agcagtagaa atcgcaaaga aatatgcaaa tcttgaggaa
     5761 accgatcatt tttattggtt tactcgaaag aagacatact tttcactgac aggtacaaat
     5821 gataaaggaa aagaaatcgc cgttatcatt cctaaatctg gtgaaaaagt aaaagtattg
     5881 gatcaaacaa agggactgac tgaaaacgaa gcaaaacaga caattgctgc ggcccatcct
     5941 gaaatccaaa tcgaaaaagc tgctttaggg atgtatgatg atcagcctgt gtgggaagta
     6001 gtaggaaaag aaacaaatgg gaaactgaat tattacctga ttggctttga atctggagat
     6061 gaagtcaaga caatcaaggg aatttaaaag gtaaaggaat gaatgggatg gagttatcaa
     6121 aaagaacaaa acgattagaa ccgtctgtca ctttggcagc tgcggcaaag gccaaggcat
     6181 taaaagcaaa aggcagagat gtgctgagtt tgactgtggg agaaccggat tttgcgacac
     6241 cagaaaacat ccaagaagca gcaatcgaag caatccgcaa tgggaaagct agctattata
     6301 caccaacagc aggaatccca gagcttcgac aggcaatcgt tgattatctg aagaaaaact
     6361 atggacttgt ctatgaacct tcacaaacag tcgtaacaga cggagcgaaa tttgctttgt
     6421 ataccctttt ccaaacgatc cttgacccgc aagatgaagt catcattcct gtgccttact
     6481 gggtcagtta cggtgagcaa gtaaaactgg cagaaggact tcctgtcttt gtgaaagggg
     6541 aagaatccaa tagctggaaa gttactgttg aacagctaga acaagcacgt tcagaaaaaa
     6601 caaaagccgt aatcatcaat tctccatcga atccgactgg aatgatttat agcaagaatg
     6661 aacttcaggc aatcggtgaa tgggcagtaa aaaatgatat tttgatagtt gctgatgata
     6721 tttatggtcg tttagtttat aatggcaatg agtttactcc aattgcaacg atttcagaag
     6781 caatcaaaaa tcagaccatc atcatcaatg gcgtgtctaa aacatatgcg atgacaggct
     6841 ggcgcattgg ttatgcggtg gggaattctg agatcatcaa tggaatgatt gctgttgctt
     6901 cccagtcaac cagtaatccg acagcagtca gtcaatatgc agctgttgaa gcattgaaag
     6961 gtgagcaaga tacagtagaa gaaatgcgga acgcctttga agaacgcctg aatacgttat
     7021 atccgcttgt ggcagaactg ccaggtgttt ctcttgaaaa gccgcaagga gctttctatc
     7081 tttttcctaa tgtaaaagaa acgctacgca tgtgtaaata tgaaaatgtc acacaatggg
     7141 tagaagatct tttagaagaa acaggggtag cgcttgtgac tggcgaaggc tttggggcac
     7201 cagaaaatgt ccgcatgagt tatgctactg atcgcgctac gttagaagaa gcggtacgca
     7261 gaatcaagca atttattgag agtaaaagtc aaaattaatc ctgaagaaat tcaaggaata
     7321 taaaaggaga gtcatacgtg gaacaaattc aaatcatcga ttcgaaaaac catgtgggag
     7381 aaacagtaac aatcggcgct tgggtcgcaa ataagcgatc aagcggaaaa atcacatttt
     7441 tacaattaag agacggaaca gcctttttcc aaggtgtagt tgtaaaaagc gaagtgtcgg
     7501 aagaagtttt ccaaacagct aaaaatctac accaagaaac atccgtgtta gtaactggag
     7561 aaatccgtga agacagtcgt tcaaaatttg gttatgaaat cggaattaca aatgtggaag
     7621 tggtaagcga gagccacgat tatccgatca caccaaaaga acacggaaca gacttcttga
     7681 tggatcatcg tcatttatgg ctgcgttctt caagacaaca tgcgattatg cagatcagaa
     7741 atgaaatcat tcgtgcaacg tatgaattct ttaatcaaca tcattttgtc aaaattgatc
     7801 cgccgatctt gactggttct gcaccagaag gcactacgga tctattcgaa acaaattact
     7861 ttgatcaaaa agcttattta tctcaaagtg gccaattata catggaagct gctgctatgg
     7921 ctttcggaaa agtcttttca ttcggtccga ctttccgtgc tgaaaaatca aaaacacgac
     7981 gtcatttgat cgaattctgg atgatcgaac cagaaatggc tttcactcat caagaagaaa
     8041 gtttagaagt acaggaacaa tatgtagcat tcttagtaca aaatgtttta gatcattgcg
     8101 aatacgcatt agatgtacta ggacgagaca aagaagtctt aaaaacttat acaaaattgc
     8161 cattccctcg tattacttac gatgatgcaa tcgaattact gaagaaaaat ggatttgatg
     8221 acatcgaatg gggcgaagac tttggttcac ctcatgaaac gttcattgcg aattcattcg
     8281 aacagcctgt ctttatccta aactatccaa aagccatcaa gccattctac atgaaaccac
     8341 atccaacaag agaagacgtg gtaatttgtg cagatatgat cgcaccagaa ggttacggtg
     8401 aaattattgg cggaagcgaa cgggcaacgg actatgattt cttgctagaa caaatccgta
     8461 atcacggttt ggatgaaaaa gaatattcat ggtatcttga tctacgtaaa tacggttctg
     8521 ttccgcactc tggttttggt ctaggtcttg aacgaacagt cacttggatt gctggtatcg
     8581 accatgtacg tgaagctatt ccattcccac gtttgttgaa ccgaatttat ccataagagg
     8641 ttgtgcgcct gccgttcagc tccgagtcac aaggagtaat ttcaaaaaac agtttttcat
     8701 gtttgatgac tattgtgact tgtggccgga gagcaacgtt tatttaaaaa aaagaactgc
     8761 gaactctcgc agttcttttt tttaaatacg ggataatgga agtttccagc agacaagaga
     8821 agaaagcaaa agaaattgct atttgattca caaggttcta taacgaaatc gcaatcaatc
     8881 aaacatttac aaagaaggta attcaaatgg aaatctgatt tgatgatgct tctgctgttg
     8941 aaataaagca gcatctagca cctgaaaaca aactaatagt aacattcgaa gatggagtag
     9001 gtccgtattt tcagcatgca ataatccata tgtaagttca attcaccctt gaaatcatcg
     9061 gaccagataa agttatgacc aatctatccc aagcaatcta gaagagatat tagtaaaggg
     9121 acatttacta gaagtcttag atgagcctat aaatatatgt ttcgatggcg tcatcagtgg
     9181 tctgattgac ggaagcttgg gtgtgatcga tctctctgga ccaaacaacg aaggaatcaa
     9241 ggggaatccg gcacgttaag aaaaaaatag cataaaaaac tagacacttg atgagcgttc
     9301 attcaattgt ttgaaggaac taggctggtg tcatttttta tacatatatt ttatctttcg
     9361 tcttgatagg gcttatggga taatataagg aagaaaatca tcgttttatt caaaaaggag
     9421 tttttgacaa atgaaaaaac gtatattatt tatcggtgat agcattacag acgcaggaag
     9481 ataccgagca gaagcaactg atctaggtaa aggttatcca cttttgattg ccggaaaact
     9541 aatctcccaa tatccaacga tggaatttga atgcatgaac cgtgggatag gaggagacaa
     9601 aattgcagat ctgcaagtac gctggcaaga agattgttta gctttacaac ctgactatgt
     9661 atcgattttg atcggaatca atgatacctg gcataatact agcgatcagg ctatattcgg
     9721 aactgagaaa tctgctcagc aatttgaatc cgtttatcga aaattactaa atgaacttca
     9781 aacagcggaa atctgcaaca tcatcttgat ggagccgttt gtacttccta taaccgacga
     9841 ccgtcatggc tggcgtaaag acttagaccc taaaatccaa attgttcgta aacttgctaa
     9901 ggaatatggc gcattgctgg ttcctttaga tggtatattc aatgctttag ggattgctta
     9961 tggtttttcg aaatatacaa gagaagatgg ggtccaccca acgattggcg gacatgagat
    10021 cattgcacaa agctggatag atgcaattgt cgcagtcgat ttttttagtg gagataaaaa
    10081 tgagcgaaag aaaagtgaaa aatgatggaa ataaggtggg cagtaaaaga agatttacct
    10141 caaatcaatc agctgattac tgatcatcca gaatcattcg taaaagcaaa aacggcagct
    10201 gacctaaatg ataagaataa actattgctc gttgctgtaa caaaagaaaa acggattatc
    10261 ggatttgcgc aagcggacgt gcaacagtca gaagaacgat tggatcagcg accatcagga
    10321 aaactaatcc aacacacatt agtaacaaaa gaaaatacgg aagaaatcca aaaaaaattt
    10381 gcagaatact tgatgctgga agctaaaaaa agaagaataa aatcaatttt tgcccagttt
    10441 taatcaaaaa cagtaataga aggagaaata caatgagttg tttaggaaat gtcatctggt
    10501 ttatattcgg cggactaatc ggtggtctta gctggttagc agcggggata ctttggtgta
    10561 tcacgatcat aggtatccca atcggtatgc aatgttttaa gatcgcctcc ctttcttttg
    10621 cgccttttgg caaagaagta atttatcacc agtctggcat gtcattgatc gttaatatca
    10681 tctggctgat cgtttcaggc ctaccaattg ctctcgcaca tgtcgtcagc ggcatccttc
    10741 tatgtatcac gatcataggg ataccttttg ccaagcaatc tttcaaactt gctcgattgg
    10801 cgttgatgcc attcggtgca gaaattgtat aaaagagaat aataaaggtt agataaagca
    10861 tagagccagg tgggatacat gtatttcacc tgactctatg cgttttcctg tataagttct
    10921 tctatgtgac ttttacgtct tttatttata tggggttact gtttatggaa agttgaaata
    10981 attttcatta aagaactctt tctatatttt tgaaagcgta gtggagcttc gtatatctgg
    11041 gaaggacatt ttgcagggat tgaaaaaaga tattgtccta tcgaaaaatt aatgaattta
    11101 gacaaattgc cagcaacagg cttcaaactt agttgctatc ctgtcaagat caagaaggca
    11161 agtgcgggtt ggataagagc agtggcaatg atcgaagaga aaaagaaaga ataagccaaa
    11221 acatctgtat ggaagaaatt ttccgatgca gatgtttttc attttttctt tcggatacct
    11281 tgacatatga ataaccattc atttataatt agttatgaag agatattcat atgttgaggc
    11341 gaggaggtca ttaaaatgaa aagttacaga ttagaaggtt tagattgtgc gaactgtgca
    11401 atgaaaatcg aaaaaggagt tcagaaaatc aatggagtca aggaagcaac ggtaaacttc
    11461 acttctggaa aactaacgat tgacgcagag gaagatcatt tggctacgat tgaacaagaa
    11521 acaaaaaaag tagtgaagga attagaacca gatgtcaaag taacagaaat agataaggaa
    11581 aaagtatctg aacatggaaa tgaaaaaaag agaaatacgc tgttccggat tttattttcc
    11641 cttgcaggta tcgctttatt gctattattt gattttaatg aaccaattcg cttgataggg
    11701 tatttattga tttatctgct gataggatat gatgtagtga agaaggcagt gatgaatatc
    11761 gtcaaaggaa aaatctttga tgagaatttt cttatgtctg ttgctacgat tggcgctatg
    11821 ttgatcggtg aatatccaga agccgtagca gtgatgctat tttatcaaat cggcgagtat
    11881 ttccaaggat tggcagtcag tcattcaaga aaatcgattc gcgaattgat ggcgatccgt
    11941 ccagaaacag ctcacgtgca gacggcggaa ggattgatga ccgtgaaccc tgaagacgtg
    12001 ctgatcggtc agtttgtact tgtgaagcct ggtgaacgtg taccgttaga tggaacgatc
    12061 atcgaaggag aatcattagt cgatacgtca gcacttacag gagaatcagt ccctaaatct
    12121 gtatatgttg gtgaaacagt attaagcgga tttatcaata aaaataaacc atttttagtt
    12181 caagtagaaa aatcttatga aaattctact atcagcaaat tattagaatt agtagaaaat
    12241 gccagcagta aaaaagcacc agctgaaaat ttcattacta aattcgcgcg ctattacaca
    12301 ccagtcgttg taggattggc cgttttatta gctgttttac ctccacttgt tgtttctaga
    12361 gcggcgtttt ctgaatggat ctatcgagca ttgacatttt tagtgatctc atgtccatgt
    12421 gcattagtga tttccgtgcc acttagtttc tttggcggta tcggaggagc aagtaaaatc
    12481 ggtgtattag taaaaggttc aaattacttg gagttgcttg cacaaactga gacagtggta
    12541 ttcgataaaa ctggcacatt aacgaaaggt gacttttcga tccagacgat cgatccaaca
    12601 ggaattgatc caaagaaatt tatgcaatat gttgcaagtg cagaacagtt ttctacacat
    12661 ccgattgctc aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc
    12721 caggaatttg caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagtaggt
    12781 aatcacaaat tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca
    12841 ttgctttatc tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg
    12901 aaagaagatg ccgtagacgc attggttcaa ttaaaacagg ctggtgtgaa aaacacagtc
    12961 atgttgacgg gcgactcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac
    13021 aagatatata gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa
    13081 agtaacaata aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca
    13141 agagcagatg taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca
    13201 gatgtagtca tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag
    13261 aaaacattga aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta
    13321 ctttccttag gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg
    13381 gtgacagtat tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga
    13441 tcaatgaaga ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg
    13501 taaagaaaat agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa
    13561 atagcttgaa attcaataag gtcaatgaaa taaaagaaaa taacttattt ttttgatgga
    13621 aaaaatatga aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc
    13681 ttgtaagttt gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag
    13741 gagagaacga atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa
    13801 catggtttgg ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt
    13861 cacctcttgg ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca
    13921 attaggatca gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac
    13981 atctaataaa cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta
    14041 tttggcacaa aagcctcaag caattctgat tgctggtggt tgggcagtcc aaggaaatgg
    14101 aaatatcgta aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct
    14161 tgccttttta tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc
    14221 cggaacagcg atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt
    14281 catcaatcca acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc
    14341 aacatttgat ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggggc
    14401 ggaaaaaata tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg
    14461 gatgatcttc ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg
    14521 tatgctgttt gctagcgata atatccctaa agacttgatg gcaaatgggg catacagtgc
    14581 ttttcaaatt ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt
    14641 gacaaacatg attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat
    14701 cttgttagca gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa
    14761 aggtacattg gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt
    14821 gccgatcttt ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc
    14881 agttgtcatg ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc
    14941 ttataagaaa ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt
    15001 tggtgcttgg tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt
    15061 agattatact gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc
    15121 aattgtcttg atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa
    15181 agaaattact gaaccagaaa cagtggttca gccttaaaat agaaatactt gttacaagag
    15241 gttgtgacag aagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgtcctg
    15301 catcaagtaa taagaacagc caaacaaaaa tagctcctca tattttgccg ttttttgagc
    15361 taatgctgaa ggtcagctga ttgaacacca tttattcgaa acaagggact ttgacacgac
    15421 ttttgtcaca gtcccttgtt tttttgcggc gttataagtg gtatgatcga accaaattcc
    15481 aaagagaagt gagggagaag aatgaagatc atcgtgattg gacaaagtgg gtcgggaaaa
    15541 tcgtccctcg caaggaagat caaagaaatc accggctttc cgcattcagg caaaaaagtg
    15601 gtttttgcaa gaacagcaat cgtttatggt atctaatccg tcatggatcg ttgaaggaaa
    15661 ttatacttct acgttagagg aaaggattaa agaagcagat aagatcattt ggcttaaagt
    15721 gccgcgttat ctcgccatct accgagtagt ttggcgttct ctaaaaagaa agataaacaa
    15781 aaaatctcgg ccagatatgc ctgaatcatt ttcggagaaa ctaaacagga aatacttaga
    15841 atttttatct tttatctggc attttgaagg aaacaatgaa ccaaaaatac agcagctgat
    15901 ccaagtagct aatgcaagaa ataaactgat catccttaaa acaagacgag agaaacaaga
    15961 atttttaact aaattgaaaa tagagagcaa aaataattga cagtttttgt ttacaagtgt
    16021 aatctgtatt cataagttaa tgattacatt tgtaaacgaa aggtgtgaaa taaatggaaa
    16081 aagtcaaaac gccagtaaag attagtgatt cagagtggga gatcatgcgt gttatttgga
    16141 cacttggaca gacgacagcc caagaaatca cgcagatctt agctgattcg atggattgga
    16201 aaccagcaac gatcaaaact ttgctgggtc gtttggtaaa aaaagaagtg atctggacag
    16261 aacaagaagg aaaaaaattc atttatcatc cagctgtctc ggagatggaa acagttcgca
    16321 gtgcgacgga aaatcttttt tcccatatct gtgcaaaaag gattggagag acgattgcgg
    16381 atctagtaga agaagccact ttgactcaag aagatatcaa tgagattttg aaacaactgg
    16441 aacaaaagca accagtagat acgatcgaat gtaactgtat cccaggtcaa tgtgaatgta
    16501 aacaacatca ataggaggaa gaaaaaatga aacaacaatt ttcaatcgaa ggtatgagct
    16561 gcaatcattg tgcagcaaga gtagaagaaa cagtaagcgc acttgacggc gtgcaaaaag
    16621 tgaaagtaaa tttgaaaaaa gcaaacggta cggtcaagtt tgatgaaaca aaagttcaat
    16681 ccgaaaagat ttgtcaagca atcaatggat tgggttataa agcagaggtg atttgatggc
    16741 aacagcagaa accaaaatgg atacgttcgt cattacaggt atgacttgtg caaattgttc
    16801 tgctaggatc gaaaaagagc tgaacggaca acctggtgtg gtcaatgcca cagtcaactt
    16861 ggccactgaa aaagcaagtg tcaaatatga agggacgacc actgaaaaat taatccaaag
    16921 cgtagaaaac atcggctatg gtgcaatttt gtacgatgaa gcccacaaac aaaaaattgc
    16981 agaagaaaag caagcgtatt tgaaaaaaat gctattcgat cttatattga gtacggtttt
    17041 gactttgccg ttgatgttgt caatgattgc tatgatgtta ggaagccatg cggcgattgt
    17101 tcactttttt cattttccaa tcgtccagtt agtgctgtct gctcctgtcc aattttatgt
    17161 cggtgctcgt ttttataaag gtgcgtacca tgcaatcaag tcgaaagcac caaatatgga
    17221 tgtattggta gcaattggaa catcggctgc ctttgcattg agcatttaca acggattttt
    17281 ccgtggacac ccccaagatc tttattttga aagcagcagt atgatcatca cgctgatttt
    17341 gctcggcaaa tatttggaac atacagcaaa aacgaaaaca ggtaatgcaa tcaaacaatt
    17401 gatgtctctt caaacaaaaa ccgcccaagt gataagaaac gggaaagaag aaacgttagc
    17461 gattgaagaa gtagtcgttg gtgatcaact agtcatccgt cctggtgagc agattccagc
    17521 agatggtcga attatttcag gaagcagtgc gatagatgaa agtatgctta ctggtgaaaa
    17581 tcttcctgta gaaaagaatc cggatgacac gctttttggc gggacaatca atacgaatgg
    17641 tctgctacac atggaagtca cacaagtcgg caagcagaca gtattagcgc aaatcattca
    17701 aatggtggaa gatgctcaag ggagcaaagc cccaatccaa aaaatcgcag atcggatctc
    17761 agggattttt gttccgatcg tattagtcat cgcgtttatc acgctcatag ctacaggttt
    17821 gatcactggt gattggcaac tcgcattgat ccatagtgtc tctgtattag tcattgcgtg
    17881 tccatgtgca ttaggtttag cgactccaac agctattatg gtaggaacgg gtgttggtgc
    17941 acgaaatgga attttaatta aaggcggaga agcattagaa gcagctgctc acttagacag
    18001 cattgttttg gataaaactg ggacgattac agaaggaaag cccaaagtta cagatctagt
    18061 cggttctaaa gaagtcctat ctatttttta tactttagaa caagcttctg aacatccact
    18121 aggtaaagca atcgtggaat acggaaaact gcaagaagca gcaacatacg atatgattga
    18181 ttttacagct cacccaggtg ctggaatcag cggtacgatt aacggagtac gctattttgc
    18241 gggtacacgt aaacgattaa ttgaattgaa cctatcattt gatgaatacc aagaacacgc
    18301 gttagaatta gaacagcagg gaaaaacagt catgttttta gctgatgaaa agcaagtgat
    18361 cgggttgatt gccgtggctg atcaaatcaa actagaagcc aaacaagcaa tcaagcagct
    18421 ccagaataaa ggattagatg tttttatgct gacaggagac aacaaactgg cagccgaaac
    18481 gattggtaaa caagttggta ttgatccaaa gcatatcttt gctgaagtac ttccagagga
    18541 taaagcagcc tacgtagaaa aacttcagaa agatggcaaa aaagtcggta tggcaggcga
    18601 tggaatcaat gatgcaccgg ctttagcatt agctgatgtt ggtatggcaa tgggaagtgg
    18661 aacggatatc gcgatggaaa cagccgatgt gacgctgatg agtagcagtt tggcatccat
    18721 tgcacaaaca atcgagttgt ctagggtgac attgagaaaa atcaaacaaa atcttttctg
    18781 ggcttttgtc tataatacga ttggtattcc ttttgccgca ctaggctttt tgaatccaat
    18841 cattgcagga ggagcgatgg cgtttagttc cgtcagtgta ttgttgaact ccctgagctt
    18901 aaatcggcac atgaaaaagt aagagaaggg atggagtaaa aatgaatggg aacgatatgg
    18961 agaaaaaaca tgaacataaa aaagaagtag taaacaaaga aagtacagaa tctcatgaaa
    19021 aacagatgga gatgaaacat gaccatagcc agatggacca ttccatgcac atgggacata
    19081 accatggaga tatagcaaac agcaaacaaa tggaccattc gatgcacatg gatcacgatc
    19141 atggcggcat ggatcattcc atgcacatgg gaaatttcaa acaaaaattt tggctatcgc
    19201 ttatcctagc tatcccaatc attctttttt caccaatgat gggaatggaa tttccatttc
    19261 aagtaacgtt tcctggttct gattggctcg tattgatctt agcaactata ctatttatct
    19321 atggcggtca gccattttta agtggagcta agatggaatt gaaacaaaaa agtcctgcta
    19381 tgatgacact gatcgcgatg gggattacag ttgcatatat ttatagtgtc tattcgttta
    19441 tcgcaaatct gctcaatcca catacacacg tcatggattt cttctgggaa ttggctactt
    19501 tgatcgttat tatgctacta ggtcactgga ttgaaatgaa tgctgtgtcg aatgccagca
    19561 atgctttaca aaaactagca gaattactgc cagaatcagt aaaacgcttg acgaaagatg
    19621 gaaaagaaga gacagtttca ttgaaagaag taaacgaagg tgatcgtttg atcgtccggt
    19681 ctggtgataa aatgccaaca gatggtgtaa tcttgaaagg tgaaacaatt gttgacgaat
    19741 cagctgtgac tggcgaatca agaggaatca aaaaacaagc caatgacaaa gtgattggcg
    19801 gttctatcaa tggggatgga acgatcgaaa tcgaagtaac tggaactggt gaaaatggtt
    19861 atctagcgaa ggttatggaa atggtgcgaa aagcacaagg agaaaaatcc aagctagagt
    19921 cattatctga caaagtggca aaatggctgt tttatgtcgc attaattgtg ggtatcctgg
    19981 cattcatcgc ctggttgttc ttgacagatc ttcctaatgc attagaacgt atggtaacag
    20041 tctttatcat cgcctgtcct catgctctag gtttagctat cccattagtt gttgctcgct
    20101 ctacatcaat agcagctaaa aacggattac ttttgaaaaa ccgcaatgca ctggagcaag
    20161 ccaatgatct agatgtgatc atgctggaca aaacaggaac actaacagaa ggaaaattta
    20221 ctgtaacggg tgtcgaagta cttgatgatg cgttcaataa aaatgaaatc cttcaatatt
    20281 taggagcatt ggaagcgaat gccaatcatc cattggctgt tggtattatg aattatttga
    20341 aagaacatga aatcaagcct tatcaagccg aaaatctaaa aaatctttca ggagttgggc
    20401 tagaggcaac agtcaaaaac caacaagtga aaatagttaa tgaaaaagaa gttgagcgtc
    20461 tcggattaaa cgtagaacaa gcattattga aaccttacca agaacaagga aataccatca
    20521 gcttcctgat tttagaaaat catctagctg caatcgttgc tttaggtgac gtagtaaaaa
    20581 cagaagcaaa agaatttatc cgaacattga aagaaagaaa aatcactcct gtcatgctta
    20641 ctggtgacaa taaaaacgct gcccaagcag tagcagatta tttagggatc gaagaatatt
    20701 atggcggttt attaccagat gataaagaag ctgttgtcca gaaatacctt gaccaaggga
    20761 aaaaagtaat catggtgggt gatgggatca atgatgcgcc aagtttagcc cgtgcttcaa
    20821 tcgggatggc aatcggtgca gggacagata tcgcaatcga ttctgcggat gtcgtattga
    20881 caaacagcga tccaaaagat attttgcatt ttcttgatct agccaagcaa acgcgaaaga
    20941 aaatgatcca gaacctctgg tggggggcag gttacaatat tcttgcaatt ccgctagcag
    21001 ccggaattct tgcgccaatt ggtatcatat taagtccagc agttggtgct gtcttgatgt
    21061 cgttaagtac ggtagttgta gctctaaatg ctttgacatt gaaaatcaaa taataatgaa
    21121 aaaaggttgt gacaaaaaac gtgtcacaac ctttttttcg aataatcgat actcaagcag
    21181 ctggttctcg gcattccaga agtagattct ccagaaattt tatcttattt tttgaagcgg
    21241 ctcccttttg tcagaacttt tttgtatacc gtttttctta ttatatgcgt aatttctaaa
    21301 taggtttttt tatcgaaatt taccggatct gcttgtcgtc catatatttc tgcttcatat
    21361 tgagtagtta atcgaatgaa acgcagatct agcttgaatt cttgttctat tcgcaggcta
    21421 tattcctgca aagattctgc gggtttgcgc ggtaccaatg tttccaaatg gttaagaagt
    21481 ttttgatatg caatcgtatt agtagaagca aaaaaatgaa gatagataag ataacttgtc
    21541 cagcgcagag attttctgaa taacaataga aagaacaaca agacgtataa acacacccca
    21601 aggctgaata agacgatttt ataagctggt gtgagtgttg tttcttctga tggaatgacc
    21661 tcagatctct cactggaaat agtttctgcc atattttcgc tttcatttgg ttgttcgata
    21721 gcaggctgga cggtttcggt tgtttcagca actggttgtg aacccattgg attggcaaat
    21781 gaaggggttg gttcaaatgg aagccagcca aatccaggaa agtatacctc tggccaagaa
    21841 tgagcatgac tattgagaat gctgtattcc tcattccctt gattatctgt gcctgttctg
    21901 agcccatttg taaaaccttt tgtccagcga gctgggatac caagtgttcg aagaaggacg
    21961 accatagaag ttgaaaagtt gtcacaatag ccgacttttg aatcaaagag gaaataatcc
    22021 acataatcgt gttctttcgg agtatgtccg gtatccgttt tggaatagcg gaaatttccg
    22081 cttgttttta aatactgttc gacagcttcg actttacgaa ttaaagttgc ttcatttttg
    22141 gtcaattcga ccgccaagtc ctcaatacgt ttaggcaaac tttttggcaa ttgaagattt
    22201 gtctgctgat tttctaaatc atcagctttt aactgactgc tttttaattc ttctctcgta
    22261 aaaactaacc tttctgtttg taaagttgca gaacgtgtcg attcattcgg gacaggaatg
    22321 aatcgctggc tttcaggcaa atagtaaagc gtttgtccac tagctaatga aaaatcagtc
    22381 aaggtagtat ttccataagg ataaggtaag aaagcttgtt tttttagggt caatgtaatg
    22441 ttttctttgt cttccataac ggtttggtag ctcgtgatgt caacagggaa ttcttggatt
    22501 ggttggatgg gtgcgtcctg atcttctatc catccagaac cggtgtaatg atttttattt
    22561 tcgatcttcc aatagccatt tcctttttgg ttcgctgtaa aaacaacttc gccattatca
    22621 tataaaggtc caccaagctg ttcatcattc tcactgaagc ctgttcttgc acttccggtt
    22681 aattgatact catggagtgt atcataaagc ccttcatcat
//
LOCUS       DACEZN010000029        22408 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEZN010000029 DACEZN010000000
VERSION     DACEZN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.029"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(52..1836)
                     /locus_tag="IXH60_000861"
     CDS             complement(52..1836)
                     /locus_tag="IXH60_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989821.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(1851..3572)
                     /locus_tag="IXH60_000862"
     CDS             complement(1851..3572)
                     /locus_tag="IXH60_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989822.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            3672..4433
                     /locus_tag="IXH60_000863"
     CDS             3672..4433
                     /locus_tag="IXH60_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1989823.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQFLLTNTDENNIRTEYYHAWIEMKK"
     gene            5400..5668
                     /locus_tag="IXH60_000864"
                     /pseudo
     CDS             5400..5668
                     /locus_tag="IXH60_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            6340..9741
                     /locus_tag="IXH60_000865"
     CDS             6340..9741
                     /locus_tag="IXH60_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1989824.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(10165..10335)
                     /locus_tag="IXH60_000866"
     CDS             complement(10165..10335)
                     /locus_tag="IXH60_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1989825.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(10529..10708)
                     /locus_tag="IXH60_000867"
     CDS             complement(10529..10708)
                     /locus_tag="IXH60_000867"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989826.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            11095..12207
                     /locus_tag="IXH60_000868"
     CDS             11095..12207
                     /locus_tag="IXH60_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1989827.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(12285..12578)
                     /locus_tag="IXH60_000869"
     CDS             complement(12285..12578)
                     /locus_tag="IXH60_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1989828.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            12746..13060
                     /locus_tag="IXH60_000870"
     CDS             12746..13060
                     /locus_tag="IXH60_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1989829.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            13081..15060
                     /locus_tag="IXH60_000871"
     CDS             13081..15060
                     /locus_tag="IXH60_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1989830.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            15073..15399
                     /locus_tag="IXH60_000872"
     CDS             15073..15399
                     /locus_tag="IXH60_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1989831.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            15415..15933
                     /locus_tag="IXH60_000873"
     CDS             15415..15933
                     /locus_tag="IXH60_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989832.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            15933..17285
                     /gene="celB"
                     /locus_tag="IXH60_000874"
     CDS             15933..17285
                     /gene="celB"
                     /locus_tag="IXH60_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1989833.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(17369..18874)
                     /locus_tag="IXH60_000875"
     CDS             complement(17369..18874)
                     /locus_tag="IXH60_000875"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1989834.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(18966..19556)
                     /locus_tag="IXH60_000876"
     CDS             complement(18966..19556)
                     /locus_tag="IXH60_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1989835.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            19735..21381
                     /locus_tag="IXH60_000877"
     CDS             19735..21381
                     /locus_tag="IXH60_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1989836.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            21430..22149
                     /locus_tag="IXH60_000878"
     CDS             21430..22149
                     /locus_tag="IXH60_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1989837.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
BASE COUNT         7151 a         3811 c         4595 g         6851 t
ORIGIN      
        1 atcgatcatg gactaatcat acatgatcga tagcttttta gataatgttt gttacgctat
       61 ctgaaattga ctattataaa gactggcata aagcgttggt gattggagta gttcagaatg
      121 tgttcctttt tcgacgatat cgccattctt cattacaagg atcatgtctg cattttcaat
      181 cgttgacaaa cggtgtgcaa tcacaaaact tgtcctatct tttgtcacag cttccattgc
      241 ttgctgaatc aatgcttctg ttcgtgtatc tacgctagaa gttgcttcat ctaaaataac
      301 gacaggagga ttcgctagaa tgactcgagc aattgtcaat aattgttgct gtccttgaga
      361 taacgtgccg ttttcgctag aaatgatcgt atcatatccg tctggcagtg ttcgaataaa
      421 gtgatcacat tgtgcgattt cagccgcttc gataatttgt tctcttgtgg catccatttt
      481 cccgtaggcg atattatcag ctaccgttcc ttcaaacagc caggtatttt gtaaaaccat
      541 cccaaaagaa cgacgtagtt ctccacgtga aagcttggta atatcaagac cgtcgaattc
      601 gatctttcct ttattcagtt cataaaaacg catcagcaga ttgactaaag tcgtttttcc
      661 tgcaccagtt ggaccaacga tcgctaccgt ttgttttggc tttactgaaa aatcgacttg
      721 attcatcaat attttatctg atgtgtaacc aaactgtaca tttttgaaag ctatttttcc
      781 tttcggttgt tctaaaagtc ttgcatctgg aaggtctgga atttcatctg tttcatctaa
      841 tatctcaaat atcctatcga ttgctgccat tgcggattgg atcgagttga tcacataaga
      901 agcggtagaa attggttcag aaatctgatt gatgtactga agataagctt gtaagaagcc
      961 gatcgtcaca ccacctgata gaacaagcat agcaccgaat accgcactta tgatgaaggc
     1021 gagttgatta atgaaacgaa tggccggata gatagcacag ttcataaatt gagcacgttt
     1081 aaaagcgttg tattgctttt tatttacttc gtgtatagca gcttctgcat gagcttgctg
     1141 attgaatgtt ttgaccacta gattaccagc taagatttct tccattcgat tatttaaaac
     1201 acccagttca tactgattct gttcagaaat ctgtttgttt ttgttcgcaa tcttagttgt
     1261 aacaattgaa ctgccaacga tcagcaataa gacgagcaaa gtaagcttgc tttcgatata
     1321 aaacagcatc agaccagcag cagctattgt tacgacagaa gtaaaaaatt gattgatacc
     1381 tgtcaataac acttgagaaa gttgattgag accggtagtt gtccgactaa tgatgtcacc
     1441 tacttgatga ttgtcaaaaa acgacatcgg taatgttttg aatttttttg tgatttcttt
     1501 tcgcaccctc aatgtgatct tttcgcttaa cgctgccatt gtccgttcct gaataaatga
     1561 agtgatgctg cttaagactg aacagccgat cagcaataag acagggaaca acaatgcttg
     1621 tttgatatca ctgaaagaaa gctgtccaat acctgttttt cttatttctg ataagagatc
     1681 atcgatcccg attcccatga tcaagggcat ggctacgact aataggttcc cgaataaact
     1741 gcatagaaac aatccgtaaa aatatttatg ttcaggtttg atcattttcc aaaaacgtat
     1801 caaagcacgc gtgttcaacg tttttttctt ttccatcgta tcccttcttt ctatattcct
     1861 tgtgattttg caaactcctg ataagtttgg caattagaca gtagttcttc atgagtacct
     1921 tgtccaacga tttttccttc atctaaaaca atgatgttat ctgcttgttg gattgtactg
     1981 aggcgttgag caacaatcac gagagttttc tccgacatgt gtttatgaag ggctgctctt
     2041 aatgctgcat ctgttttata gtctaaagcg gagaaactat catcgaatac atagatctct
     2101 gctggtttga tcaacgctcg agcaatacat atccgctgtt tctggccccc tgaataatta
     2161 gaaccgcctt gagcaacaaa gctgtccaat ccgtcaggaa gggtgtccac tagttcttct
     2221 aattgagcga tcttgatcgc tttagacaac tcttctgttg aggcatattc atttcccatc
     2281 tgcaagttgc ttaaaattgt cccgctgaat aagaaagcct tctgagggac ataactgatg
     2341 tgggagcgga tctgttcttg agtcatttga gtaagaggaa taccaccaag ggttactttt
     2401 cctatcgtag ggtcactaag tctcaagaga agtttcgcca aactactttt tccagatccg
     2461 gtcccgccaa cgatcgctgt tgtttttcct ttaggtattg tgaaatcaat atcttctaga
     2521 actggatcag agatttcagt atatttaaat gtgacatgtt caaaagcagc tgcatttgtc
     2581 tgatcaaagg ttggttcagc tactgttttg atcgtctctt cctcaatttc gatctctgct
     2641 tgcagtattt cttctatccg tcgtaatgag gcaaacgatt tagggataat gaccaatacc
     2701 atagctgcga tcatcaagta agcaagtgtc agcatagagt attcgactac agcagtcacg
     2761 acaccgattt gtaatgctcc ttctgctaca agatttccgc caaaccacaa aatagaagca
     2821 taaacgattc ccatgagcaa aaaagcaatt ggagtgaccc aagcaaatat tcggttcact
     2881 ttgatcattt gattagcata gtcagcaaag ctcttattcg ttcgcttttc ttcatatgat
     2941 tgattatcaa aggcacggat catattaatc ccagtaaaga attctctcaa tgtgatggtg
     3001 atccgatcca tttttggctg gatactcaaa gataaaggtg cccctcgttt catcagtcga
     3061 taaacaatcc atacataaaa aacaacagag atcaatgtaa cccatgttag taatagagaa
     3121 taacgaaaca tcatgagaat ggtgaaaacg caaataaccg gtgctggtaa gatcatttgg
     3181 caaaataaaa caatcatttg ctggacatta tcgacatcat tcgtcattct tgtcaaaaga
     3241 gatccagaac taaaacgact gatatccttc attgacagct gttggaattt acggaaatat
     3301 ttctcccgcg tttggaatcc aaacctagcg gaagccaatg ctgaaaagta actagaggca
     3361 actgcagcga ttgttcctag aaacgctaca agcagcattt caactccaat tttttttata
     3421 actgaggctt cgccacttgc tatacctgtg tcgatcagct ttgctactaa ttttggtaca
     3481 cctaatgtac cggcgatttc taaacagatc atcacaaagg taaggagagc gagtcctttg
     3541 tttttctgta agaatgtttt taagatagac atattattcc tccttttttc ctgatacttt
     3601 tcgattctat accttcaagt aacttgaagg tcaatagaaa aaagaataaa ctgaaaaaga
     3661 ggtgagaaaa aatgaaaaaa atgtatacga ttggccaagt atcggaacta tttgatttgc
     3721 caaaatcaac gatccgctat tgggatgaac aaggattaat ccgttcaagt agacaagaag
     3781 agaatgatta tcgattgttt gatatcgatg atatttttat gctttatgat atcgtttttt
     3841 atagaaaatt agatataccg atcaaacaga tgaagaattt atatggaaaa acattgacgg
     3901 agttatatga gacacttgat gaaacagaaa gaagaatcca taaagaactg gtggtgatga
     3961 aacaaaaaca aaaagagata agggagcaaa aaaaacagtt gaaattgatg atcgatacaa
     4021 acgaggaaga atttcctgta gaagagatcc cttttgactg tatgatctca acggagtttg
     4081 aagatattgt ggaaatcaaa aaatttctac ctaactattc aagctttggg atgatgagta
     4141 tgccagcttc cagttcacag acgatgtatg gattttttat cgaccctagt gaagtacatt
     4201 tatttactca agacgtgatt tgggagaaaa aagatacggc tgtttatcgt cgattcttat
     4261 tgaaatccga gatgaaccat gcggaacgta ataatatttt agaaatcaga gaaagaatgt
     4321 atgaaaaagg atggaaaaca ggagaagtga tcggccagtt tctattgacg aatacggatg
     4381 aaaacaatat cagaactgaa tattatcatg catggataga aatgaaaaaa taggaaatat
     4441 gtgtttaaga taactcgaaa atgatggttg ttttagttat aaagaaaaat tttctggttc
     4501 gattacgggt ttaatgtggg ggattattag tcgtggatag atagaatttt agttttttag
     4561 aggaaatatg tctttaaaaa tggaatggtt gcaattctta gcgatatata cgatgtaatt
     4621 gtcctcttaa aaaagtatgg aaaaataatt gtattgggtg ttatgtataa ttcatggtga
     4681 actgagacta atgagaagaa aaattcatat tgttatgagt gagattgtat cagatgagaa
     4741 tgataaatta aataggtgca taaaagcatt acacttaaaa tacattgttg cttacattat
     4801 tacaatatgt taataaggca aaaataaata gatggattga ataaacatat tttttaatca
     4861 aatagtccct actgtgactt tcctcagcta tcggtgatac taccgatagc atttttttgc
     4921 ttataaaata attataagta tttagagatt tttcttctaa cattcttcaa acaagaaaaa
     4981 tcacaaaggt tattgttatt tttatatatt ctttttaaga atgattgttt ttttatagaa
     5041 attttaaaaa ataaaacacg ttattgatgg atagttagtt tgttatttgg taatattata
     5101 gaagttgact ggtagtgatt tatttttcgt cgttttataa aaatgttatt ttttggaaaa
     5161 attcacaaat atgtaacgaa tgtttttctg gaatttgctg tgttaaaaag cagtatgact
     5221 aatataaata ctattgtcat ttatgcaatt ttttatggat agtaatttta gaggggaatc
     5281 aatgaaggaa gaatagaagg gaaaaatagt tgcaaatacg ccagttagca aaaatagcaa
     5341 tacatcttca agctttaaaa ttcgagagaa tcatcttggt agtggaatca actacaacaa
     5401 aaggattaaa ccaattaata gatagaatta ccaatggaaa aatagaaaag gtagttattc
     5461 tatacaaaag ttgtttaatt cgcttcggtt atgaacttag tgaaaccctt tgcaatatat
     5521 ggtacagaat atagaaatta ttgacattac agaaaatgat gaagatttaa tacagattgt
     5581 cactatttgt agataccttt tacaaggaaa ccgagccaat aaagctaaga agatgattaa
     5641 ggagttgatg gaagacgata caagttaaga aagtgaaact acgcccaaat aaagagcaaa
     5701 aagagtaatt ggacgtggaa aatgcaagaa atgaaccatc gattcggtgg acagtttatt
     5761 agtaatgacg gtttgagaac atccatgacg agaatgaagg aacatcctaa atgcgtttag
     5821 ttgaaccaag tgccaaataa tatggccaaa taagcgatca gttatcgatg aatcccaagt
     5881 attctaaact atatattcat tttgatgcta agcattgata tatttttttg ggattgatga
     5941 aggttaacca atacagggac tcactgatga aattctagag tctgaccttg gtatcaacga
     6001 tttagtgatt gtttctaatg gaaaagtata aaagtatata agtacattaa caaaataact
     6061 attagggtta cgctccagct tgtgcaaggc actggcagga ggaatcgtga aatcataatt
     6121 atggttaagc cacacggaca actaaaagtc atacaaagct atcgaatcaa tcaacagaag
     6181 acaatcggca atcattagct ttgtttacag cttacattgc aacgcttgag ttgaaccgaa
     6241 aatacgaggg aggtaacgca gccattttga gttggggaat ctttttagaa aattatcggg
     6301 taccttgttg tttagtcaaa attataaggg ggagtaatag tgaggaacaa atttcataaa
     6361 ctgttaagta tgtttctagt tatgcttatt gtgatcaatc aatttgggaa cataacggtt
     6421 ctagctgagt ctttagcaga acagccagag cgttcgctga cgatcgaagt tgatcaagag
     6481 gaggctgctt tgggtgagca gttccagttg cgagtcagag gaacaccaga cctgctaaat
     6541 gcattggtca ttgataagag tgaggatttg attgaagggg aagtgaagca actcaatgat
     6601 tacgaacgtc tgattactta ttcttcccag cattcaggta aatacgaatt gaccgcaaga
     6661 agcggtgata ttcaatcaaa tacggtctca atagaaatca gtgtaccaga agaaagcaca
     6721 agtgagtctt ttaatgtacc agaagaaagc acaagtgaat cttttaatat accagaagaa
     6781 agcttaagtg aatcttttaa tgaagccaga tcatctgagc catctataac gcaggaatct
     6841 cagattgaaa gacaagagga gctcttgaat tatgacatcg gcatctctgc tgagcgcccc
     6901 actttgccga gcaggcatac acaaacgtta aagggataca ttcaaggagg cgctggcgat
     6961 attcttgaag aatatgggga gatggtcgta cgagttccta gagcatcgat tggcttaatt
     7021 gagttcatta gagtggaggt cgctgatgat tcacctttaa agccactggc attttaccaa
     7081 gaaggcacag acgttgtttt tccatttacc tttgattatg aaggattaaa tgaagccgct
     7141 cagtcgtata cattcagtat cacgtttgaa gttgctttct ttcaatcagg cggtactgag
     7201 ccaaataatg actttcaagt gagtcttact tatgctgagc gagcagcaaa cacggaattt
     7261 tcaattacgc ctattccttc atcaagaccc attttttcga aattatacta tggctctaga
     7321 gaaatcgaca gccaaggaaa tcttgggaat ctgctgattg acgggcaaga cgtgaccaac
     7381 agtaataaat ttattttgca ggtaaattat gaaggatcac gaaaagacgg tgtagtggtt
     7441 accgatgtac taccgcaagg aacaactttg gcaccgaatc cgactacatg gagttccact
     7501 ggaaatagag aacctatttc aggtggggtt cggatcgcag aagtaacgaa tccaggtgaa
     7561 gcaaacgtta gaactcatta tgtttccgag gaatttgtgg acaaagtatc ttttaataag
     7621 gaaacgaata cacttgttgt ggactttggt aatctagcag agggtcaatc gtattttatt
     7681 gaatatgctc ttgaagtgac cgatgtcgac ttgttcaatg aaggaagaca agaggatggg
     7741 ttttctcgga atcgagcgag tatgacctac aataatcagg taatgagccg agactaccgc
     7801 ttgagaatcc ctgaaagtaa tcttccggca atctctttaa gaaaaagtgt ggacaaaaat
     7861 gttatcaatc tcaatgaaaa tgaactaacg tatactctgc atgtctcgat gttttctgga
     7921 acaagaaata cgcttgttat tgaagatgtt ttggaagaag gcatggaatt tgtgtcattt
     7981 ctatcagata ttccctcttt cattgatgcc gaagtcaata atggaatagt tcgtttcagt
     8041 gcgaataata cgattggtgg tccgacaagt tttaccctct cttttaaggt caatgtagag
     8101 gcgtatgcgg tcggcgatca aattttaaac tttgcaacgc ttgtgaatag tgacaatcga
     8161 ttgaatacga gtactgtaag aacaaggaaa atcgatgggc gtattaaagt aattaaaact
     8221 gatgagaatg gccaaaggct ggcaggagca aaatttgaaa ttttatcaag cgattcacaa
     8281 gtgatacaag aaggtataac ggcagaaaac ggagaatttt taagtgaacc gcttcctgta
     8341 ggtagctatc aagtccgaga aacagaagca ccggaagggt atcttttaga tgcgacggta
     8401 catgatgttt ttataaatga atactccata gaagaagtga cgttaaccat tgaaaacatc
     8461 cttgatacgg gtggtgttga actaattaaa catgctgaag gcagtggtga agtccttcaa
     8521 ggagctgttt ttgagttaca gaaccgagaa ggagaaacgc tacaaaccgg tctaactaca
     8581 ggagaagatg gaaaactggc aattgacggt ttagcaccag gagcatatca gctggtagaa
     8641 actcaagcgc caatagggta tgaattagat gcgacaccaa tcgagtttga gatcgaaaga
     8701 agtcagacag cggtggtaga attgacgaaa gaaaaccgac ttacaccagg cggtgtcgtg
     8761 ttgacaaaga tcgatgacca aagcggggaa atcctgcaag gagctgtttt tgagttacag
     8821 aaccgagaag gagaaacgct acaaaccggt ctaactacag gagaagatgg aaaactggca
     8881 attgacggtt tagcaccagg agcatatcag ctggtagaaa ctcaagcgcc aacggggtat
     8941 gaattagatg cgacaccgat cgagtttgag atcgaaagaa gtcagacagc ggtggtagaa
     9001 ttgacgaaag caaaccgact tacaccaggc ggtgtcgtgt tgacaaagat cgatgaccaa
     9061 agcggggaag tcctgcaagg agctgttttt gagttacaag atgccaatgg aaccgttctg
     9121 cagtcaggtc taaccacagg agcagatgga aaactggcga ttgacggctt agcaccagga
     9181 gtgtatcagc tggtagaaac ccaagcgcca acggggtatg aattggatgc gacaccaatc
     9241 gagtttgaga tcgaaagaag tcagacagcg gtggtagaat tgacgaaaga aaatcgactt
     9301 acaccaggcg gtgtcgtgtt gacaaagatc gatgaccaaa gcggagaagt cctgcaagga
     9361 gctgtttttg agttacaaga tgccaatgga accgttctgc agtcaggtct aaccacagga
     9421 gcagatggaa aactggcgat agatggctta gcaccaggag catatcagct ggtagaaact
     9481 caagcgccaa cggggtatga attggatgcg acaccagtaa ctttcaagat cgaaaaagaa
     9541 caagaagtgg cgatcttttt aacaaaagag aatagaaaag ttgctgatag ctcagatata
     9601 agaggaaaca atccaacaag cagtggaaat aagcatcttc caaaaactgg agaaaccatc
     9661 attagccagt ttatattgtc aatattgggt gtgctacttg ttttcatcag tataaaattt
     9721 agaaagaaac gaaatatatg agctaaaata aaaaattgaa taagcatagt ttttaaaatc
     9781 aactagtacc catcaattga atggaaacta ccgattttag aaggggtata caaacaaata
     9841 ttagtatcgg agtagagaag gttgaaccca accagtaaat aagtaaagac aattttgtgc
     9901 tgcgttgaga agtgaaaaat aaacctttgt agtaatacta gttaataaaa aatatacaaa
     9961 aacactgcga aaatcattct tgagtttcgc agtgtttttt gcctaatgac tcaagtttat
    10021 tgggtaatac tttgattttc tttctacaaa taatcagcga tcagttgtaa ttttttctct
    10081 agttttaatg gctctttgtc aataaggaga aaaacaagtc gggtcttttg tctcggaaag
    10141 cttcaaccaa ctcgtgaata aaggttacgc ctccttgaaa ggcgtggaaa aaccaagtaa
    10201 tcgatcaatc accaatttcg catttggtag aacatgtttg gtgagctgaa agtaggtggc
    10261 gttcatgtct gtaactaaga attctatttc tttaggatta gtgcagctaa ggaaatagtt
    10321 tgttaatcga ggaatttttt gtgtaggtaa attttctcga ttaatttttc tgtctcgcca
    10381 tccgcggaaa taaaactcag tttatcctca acggaaacgt gagaacgaaa ttcacaaccc
    10441 tcaacactca agacagaatt cttttggaag actttggtaa atagcttctc aactctttca
    10501 atgtgcgaat aacggttgtc aaagatacct aacaactttt cgaaataaaa aataacgaca
    10561 ctttttcagt cagtaaagaa gtgattttat gtctgacatg atttgaaatc gagtgccttg
    10621 gacggataaa ataactttga gcgttccaat ggcttcgaca gtttttacag gtatagcgct
    10681 gcttttttag gcgtataacc attggcatat gattatatta ttcaaagtgg acaatggttt
    10741 ccttcttctt gtttttcacc accattaatt tcctatttcc atctaccaca gcagaaccac
    10801 aatttctaca gacacatgga gcagggaaga gaactgcagc aacgatcaat gtttttttct
    10861 tctgtaaggt ttcgtaagag acctctgtaa tcatcaaatt tttgtctatt gatctcagaa
    10921 tttttttgat agaaccattc ataaagcata tcgttctctc agttgtttat tttgtggtga
    10981 tttaatcata caagaaaacg atatgttttt ttataaaaac atgaatagga aaaatagttg
    11041 acaagaaaat gacaacgcca tatgatgttc catagtattt acaacctgtc atagatgact
    11101 gtaaaaaata aaagtaaaaa gaaaggacga caacaggtgg atttttatca gtctctacaa
    11161 ctagatccgt ttattttaaa acaaaaaata cgagaagcag cttctaaaaa agaaaagtgc
    11221 atgtatattt gttcattgtt tttacgatca ttattcatcg tattgtttgc catttgtttt
    11281 atcattataa ttacaacatt atttgaatcg aagcataaac catacgctgt ggttttattc
    11341 tgtatgttga tgagtattcg ttttgttgac tttggttata agatttccca ttcgatcata
    11401 ggtttagcaa tcgtcatgtt ctcactatta gtagcaccgt atgtccagct gatcaaatgg
    11461 tcagttatgg gcatgttgat tcattttatt ttactaagca gcatattaat ggcaaccgct
    11521 agtgatccca aaatggggaa tgcttctttg tacggctttt catatctctt tattgtttat
    11581 tcgttaccta aagattcgtt gaacaagaat ttctttacac aaacaagtag cttattattt
    11641 ttgttttttt gttggttttc agttctcctt tacagaaaac atcgagaaaa gaacaaagac
    11701 aaaagcttgt ttaaagagat ttttttgaaa ggcatgtatt cgcaggaaaa aatttggatg
    11761 ctcatctatg catttggcat tagtcttttg atcgttgcag gagagtatgt cccttttcag
    11821 cgattgatgt gggcagggtt tgcattttct tctattgtat ctagctatgg attgatgtcg
    11881 attggtttca aagaacgagc agtagaccga atcattggct cattgattgg atgtgtttta
    11941 tttattggta tcagccaatt tattcctttt aactgggtcg gtatattagg tggtctagct
    12001 ttaggtgttt gttctaccta tagatataaa acagttttta attcttttgg tgctttggca
    12061 atcactgcca gtttattcgg ggttccagga gcagtaacga tccgcatttt tgagaatatc
    12121 cttggggtat gtttaggaat catgtatatt ggggtgactg aaatacttat ccgaaaaata
    12181 agagaaaaac acggtttgaa tcattagatg agatcgaata aaaaataagg aaaaagtgaa
    12241 gatacacatg gatgataaat tgctatcatt ccatgtgtat ctttttacgg taaatcagtg
    12301 aaacgttcga ctttcatttt taatttatac tttttacgga gttcgtttat ttgagccatc
    12361 atttcggatt tgtgatgctt ttcgattgct tcttcatctt tccaacggtc gatcagtaaa
    12421 agagattctg gatcttctag tggaaagtaa tagtcatatt tctcgtttcc ttcttctgca
    12481 cgaatcgctt tgacaatgtt tctttcgacc atttcttctg caaattttct ggcttgtcca
    12541 tttgtacttg tatacaaaat attcactgtg atactcacta aatcccctcc tgaaaaaatt
    12601 ctatcactag tatatcacgg tgccagaaat tattattcaa gatatgatac acattttttc
    12661 cgcaatgcta cggaaatata tattttgaat agaaaacgct tacaagatat gcttggttta
    12721 taaaaataaa aggagtgatt gatttatgaa aaaagcattg attatttgtg cggcaggaat
    12781 gtcatcctcg atgatggcct caaagacaac ggatttcttt aagcaaaaaa gagaagaggt
    12841 actcgtagat gcagtttcag ctacagaagg cgataatatg attaagaata gtgattttga
    12901 tttatttctg atcagcccgc aaacgacgat gtttttggat aaattcaaaa aattaggaga
    12961 atctgttgga aagcctgttg ttagtatccc tttccaagcg tatgtcccaa tccctacagg
    13021 catccagaag ttagcagagg tgatcgaaga gaacatatga tggaatagga gtggatgata
    13081 atgaaacaga aggaaaaatt gctgttgaga tatttgattg atcacaagaa ggagtatgtc
    13141 accagtcaac gtttagcttc tgaattgtta ttatctgatc ggacaatccg taattacttg
    13201 cagcggatca aagagttagt agaaaaaaac ggaggtaaga tcattgctaa accaggttat
    13261 ggctaccaac tgcatatttt acaaagactg acatttgact tgtttttgag tcggcaagaa
    13321 atagtacctg gatatgctag agaaccgcaa ggattttatg agtccgaaga tcgacaaaag
    13381 tatatattga accaattgct tttggaagat gccattcttt ttatggatga tttggcagaa
    13441 gagctttata tcagtcgttc cagtttgaca aaggacatgc aagaaatcaa ggagcgattg
    13501 attccatatt cactaaaaat cgtttctaag catggtcaag ggacatggat cgaaggggaa
    13561 gaaagaaatc gacggcattt tatcatggat actttttttg gaaaccatta tggaaattca
    13621 ttgaaagaat atttagggca aagccgtttt ttcccagaca tcagttttga agagctagtc
    13681 attatcattc tcgatgaaac aagagaagct aagttaaaag tttcggattt tatcatacag
    13741 aatctgactt tacacttggc tttggggatc aaacggctcc gagaagggtt tgaaatcaag
    13801 gaattaggca tcgaacaaga agtttctaag cgagtagagt atcaagtggc gcagcggatc
    13861 gttcaacgta ttgaggtaat cgcaggtgta tcgtttcccg aagaagaaat tgcttatttg
    13921 acattacatc tcatggctaa gtcgaatcac aaagaaaatc aaaatgatca ggaactatca
    13981 aaggaattag cgaaagttct gaaaagattg tctcaggaat ttcatttttc attagtagag
    14041 gatctccagc ttttcaatgg actactcgat catttgtcac cgatgatgat tcgattgagc
    14101 cgaggtattc gcattgagaa cccattgact gaggagataa agaaagaaaa tcccaaagtc
    14161 tttgacgcag taaaaaggca tttcagcaac atgccggctt tgaaaaacta taccatcaat
    14221 gaagatgaat gggcctattt ggctttgcat ctcatggcgg cattggaaaa agaaagagct
    14281 gcgcataaac tacatgcatt gattatctgt gcaacgggtt acggcagtgc ccaactatta
    14341 aagaatcgcg tagtcagtga attcggtaaa aatattacag tggtaagtgt aaaaggctat
    14401 tatgagatca atgaaacaac tttaaaagga atcgacctga ttatttcttc tattgatctt
    14461 tcgacaatgt ttttcaaaat tccagtttta catgtcagta tttttttgaa tgaagatgat
    14521 gttcgtaaaa tacgaaaggt agttggagaa tctataccct atcattcctc agatagaccg
    14581 ataccagcat tttctctaca gcaaaaaaag cagatttaca ccgaacagtt gtctgaaaaa
    14641 tttttcaaga cttatacgta cgaaccgaaa aaggaagaaa ttcttcatga tttgctgcaa
    14701 aagctgagca taaatgaaga agaaggatat ataagagaaa tgcgaaagca gatccagcaa
    14761 cgtgaaaata tgggacaaat catattcagt gatacagtgg cagtcccaca tcccgctgtt
    14821 ccagttggaa tcgaaacaaa ggtagccgtt gcattgattc cttctgggat gcagtgggat
    14881 caatatcaag aaattcattt tgttttttta gtttcgcctt cttatgtcga aaatgaagga
    14941 attacggtcg tgactaaagc aatcgttagg ttagttgacc agctaaaagt acagcaggaa
    15001 atcttagctg aacctatttt tgagaatttc agcagtaaat ttatcaaact gatcgaataa
    15061 ggaggaaaac agatggctga aaaaataaac agtgaaacac tacaagtgac ttcttttgat
    15121 atcattttcc atagcggaaa tgcacggaca ctgatacatg aagcatttcg ctcgatgcgt
    15181 aaagagatgt ttgaagaagt agaagagaag ttgcgacaag caaatgaaga gattcttcat
    15241 gcgcatcagt cacaaactca acttctgaaa gaatatgcca gcgggacaaa aatagaaatg
    15301 gaaattatca tggtccatgc acaagatcat ctgatgacga cgatgacatt attggaagtt
    15361 gcacaggaaa tgaattattt gtatcaaaaa aataaataga aaggaaggat cattgtgcag
    15421 gatacagtaa aaaggaaaat cgaattagaa aaaaagcaac ttagtttgaa aaacatgtat
    15481 ttcaatcgtt atctaatcgt acgttacctg actgcctttt tctttttcat gaacatgcaa
    15541 tggatgattc tgcttctatc agctaaaagt ttaggaagta gtttaccgat gattctgatt
    15601 ttagctattt tgcctgcagt aggagaacaa gtaaagcttt atcgaaaaca ccaaacgaac
    15661 gttccatgga caaagtggta ttttttgttc cagagtgtct ttaatttatt agtgattcca
    15721 gtgttgttta cctcaggatt tacattcttg tatcctttta tggcaaataa caatggagga
    15781 cagttatttg ttttcattct tgttgtaagc ggtatttttg ccagtgcatt tatacagtat
    15841 cgtttaaaga aaatttcact caaccaagac caacagtata tccgtattaa acaatatgaa
    15901 aaagcgttat atttaggaaa ggagaataac taatgtttga ttttttgcag aaatacctga
    15961 tgggacctat ggggaaaatt gcgcaactta aaatcgttcg ggctgtcatg gcagcgggga
    16021 tggcttctat tcctttcacg attgtaggct cgatgttttt ggttttaaat attttgccat
    16081 taccgttccc atttttagaa ggatttttta atgcaacttt tttcaaagtt agtgacttgt
    16141 acatgatcgt caatacaatg acgatgggga ttttatctat ttactttgcc attgtgtttg
    16201 cttatgaatt aacgtcaatt gaacgagatg aacaaaaatt aaatgttaat ccgctcacgg
    16261 gggcattact atctgttttt gcattcttta tgtgtatccc tgaattgatc atgactgatg
    16321 gtaaaatcag cttgattgct tctatcaatg agacagaaac tgtagtaaat ggtattagga
    16381 tgggctcttt tgtcgaacgt ttaggaactt caggaatctt tacagctatt attatgtcga
    16441 ttattgcggt cgaattgtat tgtatgtgtg tcaagagaaa ttgggtcata aaaatgccag
    16501 atgttgttcc gccaggagtt tctcgttctt tcactgctct gatcccaaca tttgtcattg
    16561 cgtttgtggt gatgatcatc aatggattat tagttgcact aggaacggat atcttcaaaa
    16621 tgattgctat cccatttggg tttgttacgc agttgacgaa tacatggctt ggtgtcatgg
    16681 tgatttattt cttgatccat gcattgtgga tcgtagggat ccatggagcg aatatcatca
    16741 cttctttctt gaccccgatc gtattagcta atatggcagc taacgcacaa ggcgcaaatt
    16801 atccgttggc aggagaattc aacagttctt atgtaaccgt tggtggatca ggtgcgactt
    16861 taggattgat catctttatt gcattcctcg cgaaatctga tcagctgaaa gtgttaggaa
    16921 aagcctcact tgtaccagga ttattcaata tcaatgaacc aatcattttc gggatgccaa
    16981 tcgtctacaa tccctattta gcggttccgt ttttcttagc tccgatggct tctgctacac
    17041 tcgcttattt tgccattcgg ctggaaatcg tcaaaccgat gcttgcacag atgccgtggc
    17101 cttcaccagt cgggatcgga gcatttgttg gtagtggtgg tgattggaaa gctgctgtgt
    17161 tagcagtcat ttgtgcaatc atcgcattca ttatctggtt cccatttatc aaattctatg
    17221 ataataaatt attaaatgaa gaaagagaaa aggcagcaga attagctagt gaaaagttaa
    17281 aatgatttct aagaaaaaag gaactgtgac aaaaaatcac agtccctttt tttcttagaa
    17341 atgggaaacc actagtatat aaataccatt attcttcaat caaccgcttc acgattgcat
    17401 aatccccttc ataaggttca tctacaccat tgattttttg atagggatcc acgccttttc
    17461 ccgcaataac gactgaatct tgcggagcgc tttctgctaa agcgcgtcga atcgcttcag
    17521 gacgatccat ttcataatga acgatcaaat ctggattagt aattgcttca ttgatttctt
    17581 ccgcgatttt tttcggatct tcaaatgctg ggtcatctgc cgtcaaaaag gcgatgtcag
    17641 ctgtttctga tagcactttt ccaaaatcat gtcgacggga gattgctttg tttcctgggc
    17701 tgcctaatac aacaattaca cggccatctg gatgttcatt tttggcaaat ttcagcaatg
    17761 ttttcaagct tagataatta tgtgcatagt ccacatagac atgcgctcca ttggattgga
    17821 tcaattgatc cattcggcct ggtacacgag cttcttttaa tccgtcttgt gcatcttgac
    17881 tggaggcacc tgttagtgct gcggcaatga tcgcactggc tgcattttct tggttgaatc
    17941 ctccagccag caaaatatca taagcagtat cgcagatagc taagcgatct tcatgagaag
    18001 ataaagtaaa accgtgagta cctttatctg aacggatcac ttgataattg ctttcttccg
    18061 aacgaccata agtgatcgtt ggaatctgga atagatcggc tgtttcccta agtaaatcaa
    18121 aatagtcact ttcatgattt aaaacaactg tttttgaatt caagatcaat tgtcgcttgc
    18181 aatagaaata gtcatcaaat gtcggatgtt cgatcggact gatatggtca ggcgagatat
    18241 tcaagaagat tccgacatct aaagtcaagc cgtatacgcg ttgtgttttg taagcttgtg
    18301 aagaaacttc catcactaga tgagtcatac cattttcaac tgcttctgac atcatacgat
    18361 ataaatctaa tgactcaggc gttgtcaaat gggatttgaa atatgttttg ccatctaacg
    18421 ttgtattcat agtagaaagc aatgctgttt ttttatttgt tgtgtgatcc aagattgctt
    18481 ttgtaaagta cgcagcagtt gttttacctt ttgttccagt gaatccgatc actttcaatt
    18541 tattttgcgg atagtcgtaa aaagccatag caagaagcgc cattgcttta cggatatccg
    18601 ttacgatgat tcctactgct gaattttcgt atggttgttc tgatacataa tattgaatac
    18661 cctgttcgat tgctgaatcc aaatattctt ctttgaaatt cattccttta cagaaaaaca
    18721 aagtagaagc atcagctttt cttgaatcat aacttaactg aagaaattca gctttttctg
    18781 gtacatcaag atgccagcct cttgttgaaa caaattcttt taatagattt tcttttaata
    18841 gtaaatgacg gatgtcttct aaagagaagc tcattgattg atccctcgct ttattcaatt
    18901 tcttggttta ttatagcaca ctctttttaa tatatcgttt gtttccgcta tgacaaaatc
    18961 cagctttatt caaattttct tgacacaggg gacaagcgga tgagtatttt cataaacaat
    19021 gaactgactg taatagatag gagaacgaat agtgtctcca acaatccaaa aacataataa
    19081 ttcaaaaaga cgataagact attggtaagt aaaagtcctt gcggcattgg aagccgaaaa
    19141 aatttgtgga aaataatcgg cggtatcgtt ataccgccat ttgaggcccc gactgtgtac
    19201 aatgtgtaaa ccccaagact gaacaaaaga cttcctgaaa tcagtgaaaa gaaatgtgag
    19261 gagatcattt ccacttttgg aacaatctct aaaacaacag gaaaaagcag acttcccgct
    19321 acagtcttaa aaaatactga ttttcctaat aggacagcgg ctagaagcag catcataaga
    19381 ttgatgctcc aaacagtaac tgagcgttgg atagaaaagg ccgtttcaac taaatgtccg
    19441 atggcgccca ctccggcgga agctatatcg tgagggagca agaaccaatt caatccaact
    19501 gtgataaaga aaagacctaa aaatataacg ggtagttttt ttatttcttg tgtcattgat
    19561 atctcctata aatgttagcc ataaaaggct gtatatttct ttactttacc aaaaaaacga
    19621 aaccgaattc aaaaaaacaa ttgttcttat aaaagttata taaaatcgat tgccagtttc
    19681 tgttttacca attaaaatga tagtaatgtt ttgtagaaag ttggaatctg accgatggat
    19741 aatcaaagaa aaccagcatt agatcaatta acaccgcaag aaaaaatggc acgaggctct
    19801 gcttggatga ccgccagtaa tatcatttca agattgctag gcgctgtcta tatcattccc
    19861 tggtatgctt ggatgggcga aaatgctaag gctgccaatg gattattcaa tatgggatac
    19921 aatatatatg cattgtttct actgatctct acagcaggaa tacctgctgc gattgcaaaa
    19981 caaactgcac gctataattc attgaatgaa tatggcactt cacgacgatt atttatccgt
    20041 gcattacaga tgatgggcgg gttaggcctg ttgtttgctt tgttcatgta catcgcctct
    20101 ccttggctag ctcatgcttc tggtggcgga gaagaactga ttccaacgat gcgttctttg
    20161 agtatcgctg tattggtttt tccttgtatg agtgtgatac gagggtattt gcaaggcaat
    20221 caagaaatga tgccttatgc gctgtcgcag attgtagaac aggtggcacg tattttttat
    20281 atgctattgg ctacattcat catcatgaaa gtcatggatg gtgactatgt gacggcggtc
    20341 acgcaatcaa ctttcgcggc atttatcggt atgctagcaa gtattcttgt ccttttgtat
    20401 tttttgaaga aacatcaagc gtatacatcg gcgtttattc actacagcga aaacaaaata
    20461 aatattgcaa cgaaggaatt gttgttagat accgttaaag aagcgattcc gtttattatt
    20521 gtgggttcag gggtaacgat cttcaaatta gtcgatcagt tcacttttat gaagatcatg
    20581 tctgcatcca ccgagtattc caatgcacag ttactggatc ttttttcgat ttttagtgcc
    20641 aatccagata agttgacgat ggtagttatt gcattggcaa catcgatcgc gtctactggg
    20701 ttaccgttga ttacagaagc tgttacgttg aaagatcgag taggtctggc aaaattgaca
    20761 agcggaaatt tacagttgtt ttcatttttt atgtttccgg cgacatttgg tgttatgtta
    20821 cttgcctatc cgctaaatac attattttat acaccggatt ctctaggaag taatgtactg
    20881 atccaagcaa gttttgtagg actcttccta ggactatata tgctagtttc caacatgctg
    20941 caaggaatgt ttgaaaacaa ggcggcaatt aattatttgg ttattgggtt tctagtgaaa
    21001 ctccttcttc aatacccagc cattcgttta tttgaagtat acggcccttt attggcgaca
    21061 atgatcggat ttgctgtgtc ttgtaccttg atcttaaaga gaatccatga agtagcccgc
    21121 tttaaaccaa gttttgtctg gcgcaggaca ttgctgatct ttattcttac cttgattatg
    21181 cttttggctt cgtggatcac aaagatgatt tttggtgctt ttttaaacga agatagcaaa
    21241 ttccagtctc tattgttgat tcttgtagta gcaggtatag gggctttagt gtatggttat
    21301 ttagctttga aaatccgctt ggcagaaaga ctactaggac caggaatagc gcgtttacga
    21361 cataaattaa aaataaaata agtgaatcag aagagctaga ttgatgctct tttgaaatgg
    21421 gggaaacgca tgcgtttaga taagttttta gcagatatgg gacttggcag ccgcaaagaa
    21481 gtcaaaggat taatcaaaaa aggaacgatc gaagtcaatg gacaagtggt gaaaagcgat
    21541 aaagcgcaag taaatgaatt cgaagatgcg gttacttatt taggagaaat cattacgtat
    21601 caaaaagatt tttactacat tttgcataaa cctgcaggag tcatttctgc tactcaggat
    21661 aatcatgatc aaaccgtaat cgatttattg gaagaccagg attatcggga agatcttttt
    21721 ccagtcggcc gattagataa agacacagag ggcctcttga ttttaacgaa tgacggggtg
    21781 tttgcccatc agttactatc acctaaaaaa catgtggaga aagaatatct agctgaggtt
    21841 agcggtataa tgacagaaga agatatccgt ttatttgcag aaggactgat tattgatggc
    21901 gatgagcaga cattgccggc aaaactccaa attgatcaag tagatcaaga gacagaaact
    21961 tcaattatcc gtctgatttt gcatgaagga aagtttcacc aagttaagcg aatggtcaaa
    22021 gcagtaggaa aagaagtctt atatttaaaa agaatccgta tgggaaaatt ttggctgcct
    22081 aaagatttga ataaagggca atatcgaccc atgaccaaag aagagctaga acaagtgaga
    22141 gaaaaataat tgaatggata gctaaaagaa taagcagagt agttgtagcg ccatctgaaa
    22201 agcagacttg tccgtctgac tttcggatgg cttttttgtg aaatttcggc ttatttccgt
    22261 agaagctact tctggaacac cgtttgttcg gaataaggga ctgtgacaag acttttgtta
    22321 cagtccccat tgttccttgt gactcagagt tagatagtaa ggacacgatc tccctaatac
    22381 tcaggccaaa acgctgaatt cacaacct
//
LOCUS       DACEZN010000030        22228 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEZN010000030 DACEZN010000000
VERSION     DACEZN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.030"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(37..1188)
                     /locus_tag="IXH60_000879"
     CDS             complement(37..1188)
                     /locus_tag="IXH60_000879"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1989838.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="IXH60_000880"
     CDS             complement(1201..1638)
                     /locus_tag="IXH60_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1989839.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="IXH60_000881"
     CDS             complement(1653..3419)
                     /locus_tag="IXH60_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1989840.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="IXH60_000882"
     CDS             3683..4360
                     /locus_tag="IXH60_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989841.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="IXH60_000883"
     CDS             4363..5490
                     /locus_tag="IXH60_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989842.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="IXH60_000884"
     CDS             complement(5690..6130)
                     /locus_tag="IXH60_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1989843.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="IXH60_000885"
     CDS             complement(6141..8174)
                     /locus_tag="IXH60_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1989844.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="IXH60_000886"
     CDS             complement(8176..9879)
                     /locus_tag="IXH60_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1989845.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IXH60_000887"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IXH60_000887"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1989846.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="IXH60_000888"
     CDS             complement(10962..11987)
                     /locus_tag="IXH60_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1989847.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="IXH60_000889"
     CDS             complement(12196..13065)
                     /locus_tag="IXH60_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1989848.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="IXH60_000890"
     CDS             complement(13524..15416)
                     /locus_tag="IXH60_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989849.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="IXH60_000891"
     CDS             complement(15413..17140)
                     /locus_tag="IXH60_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989850.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="IXH60_000892"
     CDS             complement(17156..17806)
                     /locus_tag="IXH60_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1989851.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="IXH60_000893"
                     /pseudo
     CDS             18010..18306
                     /locus_tag="IXH60_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            18478..19098
                     /locus_tag="IXH60_000894"
     CDS             18478..19098
                     /locus_tag="IXH60_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1989852.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="IXH60_000895"
     CDS             complement(19190..20356)
                     /locus_tag="IXH60_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1989853.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="IXH60_000896"
     CDS             complement(20368..20721)
                     /locus_tag="IXH60_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1989854.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="IXH60_000897"
     CDS             20849..21871
                     /locus_tag="IXH60_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1989855.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
BASE COUNT         6444 a         4572 c         3942 g         7270 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttaatata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaa
//
LOCUS       DACEZN010000031        21807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEZN010000031 DACEZN010000000
VERSION     DACEZN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.031"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            286..2451
                     /gene="acm"
                     /locus_tag="IXH60_000898"
     CDS             286..2451
                     /gene="acm"
                     /locus_tag="IXH60_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1989856.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2723..3208)
                     /locus_tag="IXH60_000899"
     CDS             complement(2723..3208)
                     /locus_tag="IXH60_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1989857.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3688..4329
                     /locus_tag="IXH60_000900"
     CDS             3688..4329
                     /locus_tag="IXH60_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1989858.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4348..4983
                     /locus_tag="IXH60_000901"
     CDS             4348..4983
                     /locus_tag="IXH60_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989859.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5001..5816
                     /locus_tag="IXH60_000902"
     CDS             5001..5816
                     /locus_tag="IXH60_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1989860.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6135..7571
                     /locus_tag="IXH60_000903"
     CDS             6135..7571
                     /locus_tag="IXH60_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1989861.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7760..10207
                     /locus_tag="IXH60_000904"
     CDS             7760..10207
                     /locus_tag="IXH60_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1989862.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10437..11504
                     /gene="rlmN"
                     /locus_tag="IXH60_000905"
     CDS             10437..11504
                     /gene="rlmN"
                     /locus_tag="IXH60_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1989863.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11697..12800)
                     /locus_tag="IXH60_000906"
     CDS             complement(11697..12800)
                     /locus_tag="IXH60_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1989864.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13353..13901
                     /gene="aac(6')"
                     /locus_tag="IXH60_000907"
     CDS             13353..13901
                     /gene="aac(6')"
                     /locus_tag="IXH60_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1989865.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14447..16108
                     /locus_tag="IXH60_000908"
     CDS             14447..16108
                     /locus_tag="IXH60_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1989866.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16178..16525)
                     /locus_tag="IXH60_000909"
     CDS             complement(16178..16525)
                     /locus_tag="IXH60_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1989867.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16609..17550
                     /locus_tag="IXH60_000910"
     CDS             16609..17550
                     /locus_tag="IXH60_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989868.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17551..18594
                     /locus_tag="IXH60_000911"
     CDS             17551..18594
                     /locus_tag="IXH60_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1989869.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18600..19670
                     /locus_tag="IXH60_000912"
     CDS             18600..19670
                     /locus_tag="IXH60_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1989870.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19660..20613
                     /locus_tag="IXH60_000913"
     CDS             19660..20613
                     /locus_tag="IXH60_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1989871.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20625..21635
                     /locus_tag="IXH60_000914"
     CDS             20625..21635
                     /locus_tag="IXH60_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1989872.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
BASE COUNT         7407 a         3708 c         4564 g         6128 t
ORIGIN      
        1 atttattaat gttgcatttt tgtaagttaa aaattggaaa tatagattga aaaaatgaaa
       61 gcaataagta aaataaaatg aatatatgcg aaaaaataat ttacattagg aaaaaccttg
      121 taaaaacagt tttttggcat atttattttt tataaatatt tgtaaaataa ctatcaattt
      181 tttagttaaa agattggaaa tgagtggggg aggaattgaa gaaaggattt ttgagagatg
      241 atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga
      301 aatattattt tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat
      361 gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca
      421 acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa
      481 aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt
      541 gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg
      601 gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta
      661 gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat
      721 acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca
      781 gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat
      841 actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca
      901 gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt
      961 aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt
     1021 ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata
     1081 actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata
     1141 gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat
     1201 aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt
     1261 ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag
     1321 attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga
     1381 cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa
     1441 gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta
     1501 agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct
     1561 atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc
     1621 actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca
     1681 gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat
     1741 atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa
     1801 aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca
     1861 aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac
     1921 caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc
     1981 caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa
     2041 tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt
     2101 acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct
     2161 ataccaggta gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa
     2221 attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa
     2281 caaagaagca attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa
     2341 agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg
     2401 atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta gttagaatat
     2461 aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt ataatcgaaa
     2521 aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta
     2581 actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat
     2641 tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg
     2701 aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa
     2761 caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat
     2821 gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct
     2881 ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag
     2941 gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt
     3001 ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg
     3061 atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg
     3121 acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat
     3181 aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat
     3241 cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa
     3301 atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa
     3361 gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga
     3421 aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt
     3481 tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga
     3541 tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg
     3601 aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat
     3661 ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta
     3721 ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca
     3781 ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt
     3841 caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt
     3901 tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt
     3961 atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct
     4021 caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa
     4081 atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac
     4141 ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg
     4201 aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat
     4261 cttacattga ctggtattct aacaatcata atgaaacaag ttgaaggaaa attaaattat
     4321 taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt
     4381 ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct
     4441 atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa
     4501 agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa
     4561 atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat
     4621 atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac
     4681 accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat
     4741 ccttatcagc tttctggcgg acaaaaacag cgggtagcga ttgccagagc attggctatg
     4801 aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag
     4861 caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc
     4921 catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat
     4981 taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc
     5041 ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca
     5101 acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag
     5161 ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat
     5221 atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca
     5281 atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag
     5341 tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag
     5401 gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg
     5461 gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg
     5521 ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg
     5581 gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag
     5641 acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt
     5701 cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg
     5761 aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa
     5821 taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct
     5881 gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt
     5941 caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
     6001 caaaattttt cttactactc gaaactaagc gactttttca caacctcctt tacataattt
     6061 tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg
     6121 aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg
     6181 gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat
     6241 cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt
     6301 tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc
     6361 tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag
     6421 tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata
     6481 ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata
     6541 atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg
     6601 aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt
     6661 cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg
     6721 aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag
     6781 aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta
     6841 atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg
     6901 atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat
     6961 gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc
     7021 ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg
     7081 ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa
     7141 tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc
     7201 cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga
     7261 ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg
     7321 actatcgaat caattactta caaagccacg tcaaagccat caaagaagca atttcggatg
     7381 gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg
     7441 gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt
     7501 tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag
     7561 agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt
     7621 cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa
     7681 gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg
     7741 atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac
     7801 gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt
     7861 tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat
     7921 tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc
     7981 cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg
     8041 ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt
     8101 tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt tgttagccag
     8161 tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct
     8221 ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt
     8281 actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat
     8341 ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg
     8401 aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg
     8461 cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt
     8521 gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga
     8581 gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga
     8641 gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt
     8701 gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga
     8761 ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga
     8821 gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt
     8881 cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc
     8941 ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt
     9001 agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga
     9061 agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa
     9121 ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac
     9181 ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc
     9241 agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa
     9301 actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct
     9361 gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca
     9421 agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta
     9481 caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt
     9541 catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga
     9601 agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc
     9661 tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag
     9721 aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc
     9781 agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat
     9841 ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac
     9901 agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac
     9961 aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga
    10021 aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc
    10081 agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa
    10141 gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga
    10201 catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat
    10261 gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg
    10321 tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa
    10381 ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg
    10441 aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg
    10501 gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta
    10561 atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta
    10621 tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt
    10681 tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct
    10741 tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg
    10801 ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg
    10861 tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg
    10921 ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg
    10981 atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac
    11041 acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg
    11101 cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga
    11161 aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat
    11221 atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat
    11281 taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac
    11341 atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga
    11401 aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg
    11461 gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt
    11521 ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag
    11581 agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag
    11641 caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa
    11701 ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga
    11761 atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt
    11821 gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt
    11881 aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac
    11941 tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat
    12001 ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta
    12061 tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact
    12121 tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa
    12181 gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct
    12241 agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata
    12301 cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt
    12361 acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca
    12421 cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta
    12481 gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac
    12541 aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt
    12601 gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt
    12661 attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca
    12721 tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt
    12781 aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt
    12841 taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat
    12901 aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa
    12961 caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc
    13021 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
    13081 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
    13141 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
    13201 aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag
    13261 agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact
    13321 gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata
    13381 atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg
    13441 gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag
    13501 cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag
    13561 gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc
    13621 gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag
    13681 gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat
    13741 ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat
    13801 taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt
    13861 ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag
    13921 aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg aatttttgtg
    13981 aaattccaac ttatttccga agaagctact tttggaacac cgtgtaccag aggttgtgaa
    14041 aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg
    14101 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttattc agataaggga
    14161 ctgtgacaag acttttgtca cagtttcttt ttgtttattc ttttttatct ttgtatggca
    14221 ataatagaca atataacgaa aattatttaa tggcttttgg aagaccatgg aaaaccttgt
    14281 acaaagctat tttttctgaa aaattccttg aaacctcacg aaaaaatcat tattttatga
    14341 aaaagacaaa aaaaagtgtt gattttttaa taattattca ttaaactctt aaacaacttc
    14401 agaaaatatt aattattctg aaaattccaa taaaggggta cgtaaaatga aaaagaaatt
    14461 tacatttggt ttggttgcgg tatgcggcct tgtacttgct ggatgttatg gcggcagttc
    14521 agccacaagc agtagttcag caaaaagcgg ttcagcagat ggaggaggcg tatttaactt
    14581 agtcgtgcct caagaaatgc ctacagctga cttatcagtt gcaacagata caatcagttt
    14641 tacagcattg aataacgttt atgaaggaat ttaccgtctt gatgaagaca gcaaaccaca
    14701 gccggcagga gctagcgagt tggcagaagt cagtgaagac ggattaactt acaaaatcaa
    14761 attacgagaa gacgcaaaat ggtcaaatgg tgatcctgtt acagcagcgg attacgttta
    14821 tggctggcaa cgaacagtag acccagctac agcttctgaa tatgcttacc tgtatgcgcc
    14881 agttgaaaat gcagaagaca ttacagctgg aaagaaagat aaatcagaac tagggatcaa
    14941 agcagttggt gactatgaat tagaaatcaa attgacgaaa caaacaccat atttccaata
    15001 tctgcttgct ttcccatcat ttttcccaca aaaacaatcc gtagtggaag aaaatgggga
    15061 tgcctatgca tctgcaagtg ataaagctgt ttataatgga ccattcacat tagaagattt
    15121 tgatggacca ggaacagata cagaatggac ttataagaaa aatgatcatt actgggacaa
    15181 agatacagta aaactttctg aaatcaaagt cagtgtagta aaagaatctt ctactgcatt
    15241 gaacttattc aaagatggcc aagcagatga cgtgatcttg tctggtgagt tagcacagca
    15301 aaacgcaaac gatccagctt atacttctgt taaagaagcg cggacaagct atatcgaatt
    15361 caatcaacgt gaaaagaatt caccattcaa caatgtgaac ctaagaaaag ctatttctta
    15421 ttctatcgat cgtgaagctt tagtaaaaca agtactaggt gacggatcag ttgtctctac
    15481 aggattgatc ccatcagata tggtaaaaag tccaacaacg aacgaagact ttgcaaaaga
    15541 agctggaaaa ttagtttctt atgacaaaga taaagcaaaa gaatattggg aaaaagcgaa
    15601 aaaagaattg ggcgttgatt ctttagaatt tgatttgatg gcttctgatg atgattcatc
    15661 gaaaaaagta atcgaatatg ttcaaaactc aatccaagaa aacttggatg gcgtaacagt
    15721 caaaccaaca cctgttccat tctctgtacg tcttgaccgc tcaacttcgg gtgattttga
    15781 cgcagtacta ggcggatggg ctgctgacta tgcagaccca agcagcttta ctgatttgtt
    15841 tgtcacaggt aattcataca accgtggtcg ctggagcaat gcagaatatg acgaagcagt
    15901 taaagcagct tcatcaacag acgctggcga tgaacaagcc cgttgggatg atttccaaaa
    15961 agcaatcaag atcatctctg atgatatggg tgtcgttccg gtgtaccaaa aagcagaagg
    16021 tcacctagtg aatgaaaaaa tcaaaggtat cgttcaccat gcggcaggcg cttcatggga
    16081 ttacaaatgg acatacgttg aagaataatc gttaataccg tgagcatgag aaaaaacgag
    16141 gctgcggcaa atgtcgcaac ctcgttctca tattcactta tcctaacaaa gaaaaaagca
    16201 tgaaaatagc agaaagagca attagtattc cggcaatgat cagccaaaag ctgtacatac
    16261 cgtagctggc agacgagagc gaggaaaatt ctggtttttt ctttcggttc cttgttcgtg
    16321 caagatggac cgatcgctga aaatgatcga agaaacccga agaaaagacc cataaaaaac
    16381 cgccgataat cagaaacggc aatgaccata agaatagatc attagataat tgaatgagtg
    16441 taaaggaatg gttgatgaga ttttttatta aaataaccag aatcagtaga aaagctactg
    16501 cgtaggggat agattttttt ttcatttgat tgctccttta tttttggttc attatctaat
    16561 aatgtagcga aacatctgtc agaagtcaaa agctaggggg aaacattagt gaatagttat
    16621 atcaaatatg tattaaaacg tgtctttttc atgatcatca cgctttggtt aattgcaaca
    16681 atcacctttt tcttgatgca attgctgccg ggcacgccat atacgaatca ggaacgtttg
    16741 agtcctgaaa caatcgagat gctaaataaa caagtcggtt tggataaacc agttatcgta
    16801 caatatggga tttatctttc taatttgctt caaggagatt ttggaatttc cttccaattt
    16861 aaaaaccagc cagtcgcaca tttgttagct ggaagaatcg gaccatcctt gcagttaggg
    16921 ttacaggcaa ttatctttgg aacagtccta gggacgattt taggaacgat ctctgccatg
    16981 aagcaaaata catgggcaga tacatcttct acactcgtag cgatattagg tcgttccatt
    17041 ccaaactttg tatttgctgt tttattacaa tatatctttg caatcaaatt acaagttttg
    17101 ccaatcgcaa aatgggatgg ctttgtgtac acaatcttac caacaattgc gttggccatg
    17161 tcgccgcttg ctgactctgc tcggtttatc cgtacggaaa tggtggaagt actgcatagt
    17221 gattatgtgg aattagcaaa agcaaaagga ttgagccgtt gggaaatcgc ctttaaacat
    17281 ggtcttcgaa atagtttgat tccattgatg actttattag gaccgttagc tgtagcattg
    17341 atgaccggat cactggtggt agaaaatatc tttgctattc ctggtatcgg ggaacaattc
    17401 gttaagtcaa tcacaacaaa tgattatcct acgatcatgg ctgtaacgat cctttactca
    17461 tttatgctga tttttgttat tttgatcgtg gatctattgt acggtctagt tgatccaaga
    17521 attcgtgttt cagaagggag ccgaggataa atggaaatga taccaaagcg ttatgaaaca
    17581 gttgcagata tccctgcgga tgaatttctt ccattacaaa aaaataccga agaagaacgt
    17641 gaacagatta ctgcaccttc tttgaatttt atccaagatt catggcgccg tttaaagaaa
    17701 aacaaagctg ccgttatttc aatggtttta ctattggtca tcattttgat ctctgtgatc
    17761 acaatctttg tttctcctca tgatccaact gcccaaaacg tggcttatat caatttgcct
    17821 cctcgtattc cgggaatcaa tatcgatgga ttgaatggaa aagcaatggt tggtggtgaa
    17881 cttgttgata aatatgctca agcaaacgtc ccttcaaacg taaatttctt tttaggaaca
    17941 gacggtttgg gacgtgatgt attaagccgc ttatttatgg gaatgagaat ctcgttgctg
    18001 atcgcattta ttgctgcatt actagatgta acgattggtg tagcttacgg gttggtttct
    18061 ggtttgttag gcggacgtgt cgataacgtg atgcaacgtt ttttggaaat cttatcagga
    18121 attccaaacc tcgttgtcat gattttgatg ttggtcgttt ttgaaccagg aattttttca
    18181 atcgtggctg ctatggccat cacaaactgg ataccaatgg cacgtatcgt tcgtgcacaa
    18241 acaatgaaat taaaagatca agaatatgtt ttagctggat tgacattagg tgaatcaaga
    18301 atgaaaattg cattcaaaca tattttgccg aatatctcta gtgtcattat tattcaaatg
    18361 atgtttagta ttccatctgc tattttcttc gaagcctttt taagctttat cggtttaggt
    18421 cttacgcctc cgtcagcttc attaggaacg atgttgagcg aagggtacaa gacattcctg
    18481 tatctgcctt atctattatg gattccggca gtaactttat cggttatcat gattggcttt
    18541 aacttacttg ctgatggatt gcgtgatgcc tttgatccta aaatgaaaga gtgaagacga
    18601 tgacgaaaat tttagaagtg aaagacttag aaatctcttt tgatacgtat gctggaaagg
    18661 tacgagcgat tcgcggtgtc aattttcatt tgaataaagg agaaacattg gcgatcgttg
    18721 gggaatctgg aagtggaaaa tcagtgacga ccagaagcat catgcgcttg ctgtcaagca
    18781 acgccaatat agatgctggt caaatcctct ttaaaggtaa cgatatcgtt ggaaaatcag
    18841 aaagagagat gcaaaaaatc cgtggtaaag aaatagcgat gattttccaa gatccaatga
    18901 cctctttaga tccgacgatg cccattggga agcaagtcgc tgagtccctt agaaagcata
    18961 ataaagtttc aaaaaaagag ggacaaaaag ctgcattaga attgcttaaa ttagttggaa
    19021 tacccgaagc tgagaaacgt atcaacagtt atccgcatca gttctcagga ggtcagcgtc
    19081 aacggatcgt gatcgcgatt gcactggtat gttatccgga aatcctgatt gctgatgaac
    19141 ctacaacagc gcttgatgtg actattcaag cacagatttt ggagctgctg aaagaaattc
    19201 agacaaaaat cgatacatca attatcttta tcactcatga ccttggtgta gtagcgaatg
    19261 tcgctgatcg tgtcgctgtc atgtatggag gaagaatcgt tgaagttgga acatcggaag
    19321 aaattttcta caacccgcaa catccctata cttggggact gcttggttca atgccgacat
    19381 tagatagtgc gaacgatcga ttgtatgcta ttcctggttc tccaccggat ttattgaacc
    19441 cgccaaaagg agacgcattc tatccaagaa atgaattcgc gatgaaaatc gatgcagaac
    19501 aagaaccacc attttttgaa ctatcaaaaa cacacaaggc agcaacttgg ctacttgctc
    19561 cgcaggcacc caaagtgaca ccgccagaag aaatccaacg tcgctgggct atctttaaag
    19621 agaaacaaaa acatacttac ggtcaaggag gaattgctga tgccaaataa actacttgaa
    19681 gttaaaggat tgaaacagta tttcaatgta ggaaaaaaga atgaagtaca tgctgtaaat
    19741 gacatcagct ttcacattta tgaaggagaa acattcggtt tagttggaga atctggaagc
    19801 ggaaaatcca ctactggccg tacgatcatt cgcttgaatg aacctactga tggtgagata
    19861 ttattcgacg gtcaagatat aacgaagatc aaagataaaa aaggtttgac gaaattccgg
    19921 catgatgttc agatgatctt tcaagatcca tacgcttcgt tgaatcctcg tatgaaagtc
    19981 agagatatca ttgccgaagg aattgatgtt aatggactag ctaaatcatc tgaagagcgt
    20041 gcaaaaaaag tcgatgatct attgagaact gttggattga atccaagcca cggtactcgt
    20101 tatcctcatg aattttcagg tgggcaacgc cagcgtattg gtattgcacg agcattggca
    20161 gtaaaaccgc gttttatcat ttgtgatgaa ccaatttctg cactagatgt gtcgatccaa
    20221 gcccaagtgg tcaatttgtt gcaagatctc caaaaagaac atcaattgac ctatctattt
    20281 attgcacatg atttgtcgat ggttaaacat attagtgacc gaatcggtgt aatgcataac
    20341 ggcttgttat tagagatggg aacaagtgat gaaatctata atcacggtgt tcatccttat
    20401 acagaaagtt tgttatctgc tattcctcta ccagatccag accatgagag aaaacgcaga
    20461 agaatcaaat atcaacctga accagatgac ggacaagtac gtcaacttcg tgaaatcgca
    20521 ccagaacatt ttgtctatgc aactgagcag gaagtcccat attacgcaaa aaaactaaaa
    20581 cgacaaaagg aatctcttct ggttgctaac taggagggac atagatgcta aaagctttta
    20641 aattccgtat gtatccgaca gaagaacaaa aacagcaatt gattcgtact tttggatgtg
    20701 ctcgatttac ctataaccat ttgctgaaaa agcggcagaa aagttggcaa caaacaggag
    20761 tagccaattt ttcgctcaca cccgctacat taaaaaaaga atacccattc ttaaaagaag
    20821 tagacagtct tgctttagca aatgcacagc ttaatttgga tcgggctttt cgcaattatt
    20881 tcaagggacg tgccagcttc ccaaagctta aaacgaaaaa aagtatgtgg caatcttata
    20941 cgaccaacaa ccaaacccgt acgatctatc tggaaaatgg ctacttaaag ttaccaaaac
    21001 aaaaagaact aatcaagata aatcagcatc ggccagttga aggatcgatt cgctccgcta
    21061 cgatttctgc tcgatataat gaagaatttt atgtcgcact actttgtgat gtttcgccag
    21121 taaaaaaaga atctttagct aaatggatcg ggatcgccta tcatccgaag acattgatcc
    21181 aaacatctcg acctctagaa gtaactttac caaaatttca ccagacagaa gaaaagctgc
    21241 aacatgcaca gcggaaattg aatgtgaaag tccgctccgc tcaccataga aagatcaggt
    21301 tagatcaggc aagcaattat cagaaacaaa agagaaaagt aatggatctt tatctgaaac
    21361 aaaagaacca gcgagaagat tatcttgaac aattatctgg gaaattggtc aaacaatatg
    21421 actacttatt tgtagaatct tttccaaaag aagaggcaca tgctgatttt tctattcacg
    21481 actggcataa attaataaca aaattgcgtt ataagtccca atggtataat aaaaaatttc
    21541 ttttgatcaa taccgacgga gcagaagaaa gcaattccgt aagaaagagc caagtagtag
    21601 aaaaaatggg cagacattcg gttatcaagg gataaaaaat gaatgaaact gataggagcg
    21661 ttagggttag ccttgttaat aaaaagtctt aatgactttt gtttctagga atttttcttt
    21721 tgaagaaaag ctcactaatt atgacaaacc aataaaacaa tagcagaaaa agttcgtacg
    21781 agctttttct gctattgtta atttttt
//
LOCUS       DACEZN010000032        21598 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEZN010000032 DACEZN010000000
VERSION     DACEZN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.032"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(54..1349)
                     /gene="purB"
                     /locus_tag="IXH60_000915"
     CDS             complement(54..1349)
                     /gene="purB"
                     /locus_tag="IXH60_000915"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1989873.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1362..2495)
                     /gene="purK"
                     /locus_tag="IXH60_000916"
     CDS             complement(1362..2495)
                     /gene="purK"
                     /locus_tag="IXH60_000916"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1989874.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2479..2967)
                     /gene="purE"
                     /locus_tag="IXH60_000917"
     CDS             complement(2479..2967)
                     /gene="purE"
                     /locus_tag="IXH60_000917"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1989875.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3383..4690)
                     /locus_tag="IXH60_000918"
     CDS             complement(3383..4690)
                     /locus_tag="IXH60_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1989876.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4693..5274)
                     /locus_tag="IXH60_000919"
     CDS             complement(4693..5274)
                     /locus_tag="IXH60_000919"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1989877.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5390..5487)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5846..7137)
                     /locus_tag="IXH60_000920"
                     /pseudo
     CDS             complement(5846..7137)
                     /locus_tag="IXH60_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(7134..7757)
                     /locus_tag="IXH60_000921"
     CDS             complement(7134..7757)
                     /locus_tag="IXH60_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989878.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7760..8596)
                     /locus_tag="IXH60_000922"
     CDS             complement(7760..8596)
                     /locus_tag="IXH60_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1989879.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8613..9500)
                     /locus_tag="IXH60_000923"
     CDS             complement(8613..9500)
                     /locus_tag="IXH60_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1989880.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9516..10799)
                     /locus_tag="IXH60_000924"
     CDS             complement(9516..10799)
                     /locus_tag="IXH60_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1989881.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11482..12165)
                     /locus_tag="IXH60_000925"
     CDS             complement(11482..12165)
                     /locus_tag="IXH60_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1989882.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12183..12800)
                     /locus_tag="IXH60_000926"
     CDS             complement(12183..12800)
                     /locus_tag="IXH60_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1989883.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12810..13634)
                     /locus_tag="IXH60_000927"
     CDS             complement(12810..13634)
                     /locus_tag="IXH60_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1989884.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13627..14187)
                     /locus_tag="IXH60_000928"
     CDS             complement(13627..14187)
                     /locus_tag="IXH60_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989885.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14361..15317
                     /locus_tag="IXH60_000929"
     CDS             14361..15317
                     /locus_tag="IXH60_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1989886.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15622..16578)
                     /locus_tag="IXH60_000930"
     CDS             complement(15622..16578)
                     /locus_tag="IXH60_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1989887.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16682..17284
                     /locus_tag="IXH60_000931"
     CDS             16682..17284
                     /locus_tag="IXH60_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1989888.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17372..18871)
                     /locus_tag="IXH60_000932"
     CDS             complement(17372..18871)
                     /locus_tag="IXH60_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1989889.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18887..19411)
                     /locus_tag="IXH60_000933"
     CDS             complement(18887..19411)
                     /locus_tag="IXH60_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989890.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19709..20458
                     /locus_tag="IXH60_000934"
     CDS             19709..20458
                     /locus_tag="IXH60_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1989891.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20449..21234
                     /locus_tag="IXH60_000935"
     CDS             20449..21234
                     /locus_tag="IXH60_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1989892.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21203..>21598
                     /locus_tag="IXH60_000936"
     CDS             21203..>21598
                     /locus_tag="IXH60_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1989893.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKA"
BASE COUNT         6328 a         4670 c         3785 g         6815 t
ORIGIN      
        1 ttttggttat ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc
       61 aaacctactc gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca
      121 tcgtctattt cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg
      181 cggaactgca cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct
      241 tcttctcttg tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa
      301 ccaaatgttg cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg
      361 atattgccaa aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt
      421 cgttctgctg aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca
      481 gtgaccatgt gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga
      541 ttccttttgt gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact
      601 tcacgtgttt cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta
      661 gcaataagcg ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta
      721 gaaatttctt gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg
      781 atatttgcga acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca
      841 tgttcaaatc gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca
      901 aaagtagttg gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt
      961 tcttttgctt tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca
     1021 tttgcttgct tgatgagata gccataagca gtatctacga catccgtact tgttagacca
     1081 taatggaccc attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg
     1141 acatcatggc gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc
     1201 tcacgaattt ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct
     1261 gctaaaatct cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc
     1321 atttcagggc gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt
     1381 tgtttgattt cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc
     1441 ttgcgacctt tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc
     1501 tcaatcaagc gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg
     1561 cttatcaaac gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg
     1621 aactgactca tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct
     1681 aattcattca catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct
     1741 gaaaggctca ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc
     1801 tgatcaatcg cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc
     1861 acgaccggaa aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct
     1921 ttttcaaacg ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca
     1981 ggagctagat ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga
     2041 gtggttttca aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga
     2101 ctgacgatcg ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct
     2161 aacaggcggt cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt
     2221 aaaatcgcat ttaacgcctc tatactgacg ttttcgaatt cataggtaat gacatctgtt
     2281 cttctggcaa gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct
     2341 gcaacttgtg cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt
     2401 tcttttgcac taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt
     2461 gaaccaggca acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa
     2521 cattgaacgt ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg
     2581 tatcgcaagt agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg
     2641 tactcctcca ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga
     2701 ttggaccggt acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg
     2761 cgcggctccc cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc
     2821 atattcaaac atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac
     2881 accgaattca tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc
     2941 cataatcaca gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt
     3001 ttaacaatca ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt
     3061 attgttcgtg tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct
     3121 ttttatggat aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca
     3181 aacatgagaa gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca
     3241 cgacctcttc cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg
     3301 caaaatacga ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt
     3361 tcacaacctt tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt
     3421 ttttcaatcc attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc
     3481 catttccagt gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat
     3541 tgaaacccag cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga
     3601 aatcaacttc caacagcata cggattcctt ggactgccac catcccgaac atgactaaca
     3661 ttcctccacc aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata
     3721 atcctaaaac aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg
     3781 aaagttgaac caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac
     3841 cgcctaagat aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa
     3901 catttttccc agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga
     3961 ccatggaaat gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat
     4021 aaaagggttg tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc
     4081 ccatgaaagc agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa
     4141 tgaacccttt tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta
     4201 gcagatttgt tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga
     4261 ttgctacagg aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag
     4321 ggaatagttt tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag
     4381 cgataataga tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag
     4441 ctactgcttg gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa
     4501 ctgtcaattg caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag
     4561 tcatctgttc ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc
     4621 ctgcatacat cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt
     4681 ctttttccac gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc
     4741 aatacgcgca agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg
     4801 gaaagacttt tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa
     4861 ttcgatcaat ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt
     4921 gtcttctgaa gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa
     4981 agaataaaca gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc
     5041 gaagatcatt ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc
     5101 ttcaatggtc acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc
     5161 catttgttcc atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac
     5221 gccctcgcct aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt
     5281 tgctccttta tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt
     5341 tctgttaaag aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc
     5401 ttttacttat agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga
     5461 tatataagta ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg
     5521 cacgtgaagg cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt
     5581 ctaacactac tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga
     5641 tttttaatgc attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc
     5701 taaaaaagca gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc
     5761 cgaataaata gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg
     5821 ggaagtaatt tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta
     5881 ctgcttttcc tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac
     5941 catctacgtg agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat
     6001 catctaataa acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct
     6061 catatggctc accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat
     6121 cctttttatc tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc
     6181 ctttgctgcc attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat
     6241 acgtcaaaat cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga
     6301 tatcatcgga aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat
     6361 acatttctcg caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta
     6421 gttcgcttcc atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca
     6481 tcccataaaa gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa
     6541 aattcactag atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct
     6601 tatttcgatg catcttctga aataatctgc cccgtgttgc gtatttaaca gccattcgaa
     6661 gtggatagaa aacacatcac caatcacatc actttctatc agttccttta tcttcgtgtg
     6721 atacggtgca taacgataat taaaagtgac tcgcaggctt cttcccgtct gtttttggac
     6781 cgttattatt tcttgagctt tttcctcatc gatagtcaga ggtttttcgc aaataacatc
     6841 atatcctttt ttcattgcac gaatgatata atcatgatgc gttcgatcta ctgacgtaat
     6901 aatgacttca gccggccgta cgtcatccag tagttgctca aattgtgatg gcatacatgt
     6961 ctttactttt ttctgtccat acttgttcac aagtatccga tttgcataat ccatccttgt
     7021 ttgagataca tcacaaaaac cgactagttc acaagtttga tgatacgtag aggcaattgc
     7081 ctcataaaaa aaacgcgctc tcccaccaag tccgacttgt acatattttt tcttcattta
     7141 aagtcattct ccttttcttt taagcaaaat actgtgacaa atggcagccc tggatacagt
     7201 aatgcagtga gtaaaaaatc atgctgtata caccagaaca gtccatacaa aaggcataaa
     7261 taccacagaa accccttgac gttttttgac aacagccaga tattcctttt gatcaaataa
     7321 acaagactat ctgctttatc ttctttgcag atatcaatta gcgtactaaa aaaactatgg
     7381 gatacaaaaa gcaacagaca caatagaaaa ggaatcagat accaagctcc aatctttccc
     7441 actaataaat accaatcaac tgcaaaaata atgacaaaca gctgaaacaa cgtaatgatg
     7501 aaacacatcc gtttgatttt tcctttcctc caacatacct ttttttctgc caagtaatgg
     7561 acagatagat aataagaagg atacatcatc aaaaaataaa tcagatagac aacacgagca
     7621 gacagcacta atcctaaagt gaacaaacat cctagaacta aagcgaaaag agcattactg
     7681 ataagactgg ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag aaacatctct
     7741 tctattggct ttttcatatt cagcccttca gaccgcttgt actgatcccc tggacaagat
     7801 atttattgaa gaataagaaa atcagaaaaa ccggtagcag cgacaacgta gacatcgcaa
     7861 acatactcgc gtaagtcgta ctagatccag cgtctgcaaa taatttgatg gcaatcgata
     7921 ccgtgtaaga tctcggcttg tttaaataaa tcaatggtcc catgaagtcg tcccatttcc
     7981 aataaaactg gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga aaaataattt
     8041 tggtaaagac actatacttg ctgcatccgt caataatcgc tgattcatct aactctttgg
     8101 ggatgctgac catgaattgc atgatctggt aaatgaaaaa tgcttgtcca aaaaaatgtg
     8161 gtaaaatcag cggcacatac gtcccaacca aattcagccg attgtaaata ataaactgcg
     8221 gaatcaaaat cacttgtgca ggaatcatca tcgttactaa catgacgacg aaccagaatc
     8281 tttttccttt gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag ctcagaactg
     8341 tccctatcgt cgaaactgca gagatgacaa acgaattcct aaagaaagta aaaaatgaaa
     8401 ttccagcaaa ccccgcccat ccttttgcgt aattttccca ttgaaaagtc tctggaatca
     8461 aattcagcga accactcata atctcttgat ccgttttaaa agaacctgcg atcatccata
     8521 agaccggata aatcatgact agacctaata gcaaaataaa accgtattga aaccatttcg
     8581 ttactgtatt attcatttct tcctcctctc attcacacaa cgacccattt ttttgatgat
     8641 ttgaaaatca gaaaggtgac agctcccata atcaacagca tgatccacga aatagcacta
     8701 gcatacccca tagaattaaa agaaaaagct tggttataca cataaagagc ataaaagttc
     8761 gtggcatcat tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt cataaacgcc
     8821 gcgattgtct gcataaccaa gttatacaaa atgatcggcg aaagaagagg taacgtgatc
     8881 ttgtaaaacg tctgccattt gttcgctcca tcgatctctg ccgcttcata atacgagcct
     8941 gggatctctt tgatacctgc agcaaagatg atcattgccg aaccgaattg ccaagcagac
     9001 atcaaaacaa gagggatcat gaccagtttt tgatcgctaa accaggaaag cgcaggtgcg
     9061 cctaatcgtt ccaggaaaat attcactaac ccgtttttag aaaacaactc ccgccaaact
     9121 agagcgatag aaatactgcc accgatcaaa ctaggcacat aatacaaaga acgaaagaaa
     9181 gtcgttcctt tgatattcct cgttaaaaga taagcaatca atagaccaaa gatcagtttc
     9241 acaggtacag atgcaaatac atagagaaaa gtgactcgga tggagttcaa aaaacgggca
     9301 tcactcagca tctttatata attatccaag ccgatccatg agactggatt tcccaaacga
     9361 taatcggtga acgaataata aagtgacaag atcatcggta tcactgttat caatagaaat
     9421 cctaggatga atggagaagc aaatacgtaa ccgaccacat tgtcttgatc caagaaccgt
     9481 tttagttttt cttttttcat gccttcctcc tgctgctatc ctagaatgtt tgacgcaaac
     9541 tcataaagac tctctgctgc ttcttctgct gttacttctt cataaatgac tttttcaact
     9601 aaaagattgt attggtcttg aatttccgga ttcaaagcac tatcaaagac attcacttcg
     9661 ccgttgtcca tatcggaaat catttctaaa taatcaaatg tcgcttgcaa agttgcatca
     9721 tctgcacgag cgagctgttc tcgaatattc gacatgattg gtacaccacg ttctcctttt
     9781 aaaatttcat tggcttcttc actgttttga aagaaatcaa taaacttagc agcttcttct
     9841 ttatgggctg agctttttgc catactcagc atttgcgaag aacgtaaagg gataccagat
     9901 tctccatctg cttttctgcg aggtggatta atcaactgaa tctccatatc ttcaggagcc
     9961 cctgacaata tttctgtcaa ctggttactt gatagaaaca ccattgccgc ttctctatcc
    10021 ctaaccgcct gaaaagacag atccgtcgta cttgcagctc caccacctgg ttctggatgg
    10081 gcccctttgt ctaccagatt tttacgcagc tggaaaaaat ctgttagata gtctggattt
    10141 tcaaatccca agctgttaga tagatcttcc cgattataaa aattcaatcc tttctcaact
    10201 tgtggtattt gaataataca gccagctata aaatcttcga atcttgaaat agcatagatt
    10261 cctaattctt cagtgatttg atagctgatt tgttcaaatt catcccatgt ccaatttgaa
    10321 gctggttcag ggatatttaa ttgttcaaat atatttttat tgtaagcaat cgcaaacgaa
    10381 ttcaccccgt tagacagtcc aactaattgc ccgtcgtaag tcgtcgttgc taaataggat
    10441 tcatctgcat tagaaatatc aattatccct tgctccacat attcatctaa tggttcaatc
    10501 tgattgatgt attgaggaaa ttgattgttc agttggaaaa tgtcccagat atcatctgat
    10561 gctgctagtg tacctaattt tgtgtaatat ccttcataat cataaaattc ataatcgatc
    10621 gtcacatggg gatttttttc ttcataaagc tcgattactt ttactgttgc atcgtgtcgg
    10681 acttgcgaac cccaccaagc catgttcagc gtgatttttt tgtcatcttc ttgcccacaa
    10741 gccgtcaaga agaataacat aacacttaaa gctaagatga ttctcattct tttcttcatt
    10801 ctctgcacct ctacttccat tgttattttt caatatactg tatttttata cagaacatat
    10861 ttattaaacg atctttttca aggggaataa aaaccaagac ttgccttatc gttctgccta
    10921 ggatgaacaa ctcaatactt tcaagaaaaa ttagatagtg ctgattcttc tttcatcgct
    10981 aaaaacactt cttgcttcaa tgatgggagg ataattacgc aaagaaaaaa ggatatcaga
    11041 caactgccca atatccttga catagctaaa taagcatgct tcttcgtgtg cgctcttccc
    11101 taaaaaacag aaaaataact agtaattctg tatgaaatta ggtacaatat acacgtcgat
    11161 gcacaagcat tgactatggc aacttgattg gtcagttccc gctgactttt caagccccga
    11221 caatttagct gctactaaat tgtcgggtgc cttttttagt ttttattcgt ttttcattct
    11281 atagaaagcg cttcaataaa tcaagacatt tttttgttag aatgctaata ataacaaaag
    11341 gttttaactc ttcttatgta cattacaggt aaaaaaacaa aaaaatttct cttttttttg
    11401 aattctgaac aaatagtact ggagtacaaa agacgaggaa gagccactat ctgctcttcc
    11461 tcgtcttttg attctcacct gttattcatg attctttttc tcagtttccc gatatttttc
    11521 cattttacga ctaaaaagct ctccgctact tggtggcgta tatgtgatgg catttgctcc
    11581 tgcttgaatg gtctcagcaa tactttcttc cgttggacca cctgtcgcaa tgataggaag
    11641 ttcaggatat tttttacgaa aatggcgtac tgcttctgca gtttctttac ctgcactgat
    11701 attgataatc gaaataccag ctgctaactt ttcatctatt tctgtatatt tcgacgttac
    11761 cgtactgata actggaatat caactactcg acaaacttct tttactgtct ctaaaggcgt
    11821 aggaccgttc aaaacgaccc ctaacgaacc ttgcgcttct gcgaacatgc tcatgtagga
    11881 agaacgagca ccttgtgtca aaccaccacc tacaccggag aaaactggaa tgtcagctgc
    11941 ctcaataatg cttttagtaa tcgctggatg aggtgtaaaa ggatacacag caattactgc
    12001 attcgcattt gtattacgta tgatcgcaat atccgtagta aagatgatcg aacggatcaa
    12061 ttttccaaat atcttgatcc cgcttgcttc taaaatgatt tccggtactc ttacgatatc
    12121 ttttctcaat tccgtcatga tttctggcgt aaattgttct gccatcagcc acttctcctt
    12181 tgctatctcc ggtcatactg attcaattga tacgtctcga tgacctggat gattttttgc
    12241 gcataaccgg ggtctgtcgc ataacctgca tcttgcaatg cttgtgcggc ttcctgatag
    12301 ttggtggccg taatcacttt ttcatacagc gcaggattcc aatctactcc ttggacgaac
    12361 agttgtgtat ggtcatccat ggactgttcc catgaatcat acactttgaa gtctccttgg
    12421 atagttatcc attcttcgtt aacaaattcc tttgtttcca ggcttacttt cttctgatcc
    12481 ccataagcct tgatcccgaa aagattattg tatttgctag ccaaggtact ttttccccaa
    12541 tttgattcta atatagcttg tcctaaaatg atactaggaa gcactccata accatcttgt
    12601 agttcttggg catgcggaac aagccgttca ataaattgct gatgggattg cgcttcattt
    12661 tcttgctgat tatcagtaaa aggagaagac agattcttca aggaaaaaac aaaggctaag
    12721 agtatcactg ctacaccggc aaccactgct ggccaacgga tattcttgcg cttttgcttt
    12781 ttccactttt ttttcgccat gatcaattcc tacttttcat ttaattcatt cagatattct
    12841 tctagcgtca attggtgttt ggtgatatac tcagcgtttt ctttaccgac ataacgtaag
    12901 tgccaaggtt catatgtgat cttagttata tcttcgtgtc cttttggata acgaacgata
    12961 aatccatact gcgcagcatt atctgcgatc catttagctc caggctgatc tccgtaactt
    13021 gcatccaata cagtagaagg ataactgttg taccagtttt gatcgacgac atcgactgcc
    13081 agacctgtgt ggtgttcgct gtatcctggc tccgtaatcg tttctttagt aatctttgtc
    13141 gcctcctctt ctgatacacc ttgacgactc atcacatctt ggacattttg ttcgaaaact
    13201 tgttgttggg atgatacaga ccggtaagct gagaccatca ctaatggaaa tccagctgct
    13261 tcagcagcct tggcaaatgc ttcatattct ggagcaatgc gtttgtcgat caagtatccg
    13321 ttacttaatg atgccaattg agtagactca tcaatttctt tttctaattt gtgatctgga
    13381 ccaaccaaaa ctagcttcca atcgtctgtt ttaacatctg gcaattgttt ggacgtacga
    13441 tcttttgttt ctttgtcctt taccttgctg ctggttgttt gactgttctt ttctttacta
    13501 gcagttgttt tggtgcttgt tcgttcgact gcttgattgc cggcttgaac atgcgaatca
    13561 tcatcgctaa aaataacata gcccattgct gcgatcataa tcacaactgc tccaaatcct
    13621 aaaaatttat tcacttgaaa aatcctttcg ttgttcttca cgttccccat tcgcctcatg
    13681 aacgtaagtt tcttccagat tttcattcac ccaatcgaga agttcgattt tttctccttc
    13741 gatctgtttt tgaacatctg ctggcagacg aaaattcaaa atatcatcat agccccattc
    13801 atcatattga atacttactt tcaagtcaag gtatctacca gcctgctgat tatttttcaa
    13861 agggatcagc tcgacttttg ctgctttgct gctaagccat ttttgcgcga tgcgtgtttt
    13921 gatttgcttg tactctgcaa cggcaacgtt tcgtttttcg ggaaaaataa tcgtttgacg
    13981 caaaatcttt tgcgttagca tccattcata atcagtgaaa agatttttta ctttctgcat
    14041 cgcttcagat ggcagatttt tctcgccatc tttccatttt tgccattgtt ctggactgat
    14101 cagcaagtat tcatctaaaa aatttttttc agtttcaaac tgttctaata atacactcat
    14161 aataagatca atatatactt gatgcacgtt tatcccctcc ttttaactat tctacttaag
    14221 ttttgatcaa aaagcacctc ttaaatcgct gctttcattt tttttaatgt ttcaagtcct
    14281 ctcctcttta ttcttcgtga tttttgttat aataatagac agacaagaaa ctcaatccaa
    14341 ctatgaagga gcgttttcag atgaaaaaat atcaatgggg aatcgttggt cttggtacca
    14401 tcgcacatga gtttgccgaa agcttcaatc aagagacgag cgaactaaca gcagttgctt
    14461 ccagaacttc agaaaaagcg gaaaacttcg ctcaccgtta caatattcca aaagcttacg
    14521 gaagctatca agaaatgtta gacgatgctg aaatcgacat cgtgtacatt gctgtaccaa
    14581 atagacagca tatcgaccat atcttggcag cattggaagc aggcaaacac gttctatgtg
    14641 aaaaagccat cacgatgaat aaaaaagaac tggctgacgc tatgcgctta gcagaagaaa
    14701 agaatttgat cttagcagaa gcgatgacaa tcttcaacat gccactttat cagcagttac
    14761 gctcaataat ggatacagga aaactggggg ctttaaaaat gatccaagca cctttcggca
    14821 gttacaaaga accagaccct aaaaaccggt tcttcaatcc agaattagct ggaggagctc
    14881 tgttggatat cggtacgtat gctgtatctt tcgctcgttt ctttttgagc tctcaacctg
    14941 aagtagttgc ctctacaatg gttccttttg aaacaggcgt ggacgaacaa tccgtcacta
    15001 tcttgcgtaa caaagaaaat gaacttgctg ctgtcagctt gacttttcag gcaaaaatgc
    15061 caaaagtcgg tatcgttgcc tttgagaacg gttatgtgac gattgctgat tacccgcgtg
    15121 ctgatcgtgc ggagatctta tttacagatg gtacaaagga attcatcgaa agcgggaata
    15181 catctgaagc aatgaattac gaaataaaga atatggtaca catgattgaa ggtgacttgc
    15241 ccaatcgctc tctcttcttg acaaaagatg tcatcgaaat cttggatcaa atgcaagttc
    15301 tatggaaaaa tatgtaattt taaaaggttg tgaaaaagct gttctgcatc aagtaataag
    15361 aacagccaaa caaaaatagc tcctcctatt tttcgtttgg ctggacttat taccgcagag
    15421 gaaagctttt gaacaccgtt taagaaagag gttgtgagcc taccgttcag ctccgagtca
    15481 caaggagcaa tttcaaaaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga
    15541 ggagttgaca ggagaacacc gtttaaagaa gaggttgtga aatcagcttt ttgacctgaa
    15601 tattagctga attcacaacc tctatttttg gattttttgt tgggagaatt ctgccttgat
    15661 ttcaataaaa cttttaaaag actgcaaaaa ctggaacaat tctgctcgat cctcgaattc
    15721 agggcgacta gtcggcagag gcatttgccg atacaataca taaacttctt caatacgtgt
    15781 taaaatttgt ctgccatcat ttgactctga aaatgtttct gcagtataaa tcaataaacc
    15841 atagatatgt tttccataat aagctggttg ctggattctc tctagatttt ccagcatgtc
    15901 tttaatcaca ttggcctgtg ctcttctcat agagaagtaa gtttcgtagt atagcggctg
    15961 tctgagccag tagttttctt gatgttctct ggcatcttct tggctttcgc gaatgaatgt
    16021 cagtaaatgt tcgcatttga tctgcacatc ttctttattt tctgataaaa gaaattctgc
    16081 catttgtctc aaaatatttc gaaattcttt ttcgatctgt atttggtttt ccttcagctg
    16141 cttcgtacga tctggcatgt aaacatttgc aagcaaagca aatactaatc caatcgccat
    16201 taatcccgct tcattgccga tcagctgcca agacatgttt tcctccacta aataatgagt
    16261 gaccaaaacc gaatttacta ctatcccttc ggttaatttg aagcgagagg caagcgggat
    16321 gaacaggaat aaaaaaagac tgaagccaaa aatagtatac ccaacagcaa aaaaactgaa
    16381 cagtgctgtt gccgtagcta atataaaggc tgcgattcga ttcatgcccg tacgcaatgt
    16441 agagcgcttg gtatttccta cacttaataa tgcaatgatc cctgcagctg gccaatacaa
    16501 cagcgataag ctacccgctg tgaccatcga aaagaagaca ctgatcactg ttttgactgt
    16561 tcgcattcct atacgcattt acttccccct tttttatttt aattatactt gaataatgaa
    16621 aaactagcta tcagacaaaa gtttaggtat gatagtaaaa agaccaaaaa ggagaatctc
    16681 tatgcaattt ggccgttttc gactaggtat gcgtacgatc aaaagtgcct tagctgtttt
    16741 tttatgtatc ttgttttttc atttgactca acgaggaatc ccgatgattg ctgcattatc
    16801 tgctgttttt tctttgcgtc aggacttgac tacaaccgtt tcttttggaa agtctaggat
    16861 catcggcaat acattaggtg gtacattagc tatcatttat ttttttgtca aaagctattt
    16921 tcacaatgat tttttggtag aattgatctt gcttcctatt ctagttatta ttgtgatcgt
    16981 cgtgtcagat ggtattaaca ataattccgg tatcatttcc gcaatagcta cgttgttgct
    17041 gattgcgttg agcatccctc aaggcgaatc cgctttgtat gctatccaac gtgtactaga
    17101 tacttttata gggacattca ttgcaatcgg tatcaatttc tttcttcgtc ctcctgaaac
    17161 agaaaagaag gaagaactat cagaagattt ggtcgaactc caaaagaaag aacgattgct
    17221 aagaacaaat ttacaagaga tagaagctaa gatccaaaaa caaaagaaaa atagcgagga
    17281 ctaattttct tttgcaataa aataaaaaaa tggagagaag aactttaaaa agtcttcctc
    17341 catttttgat tgaacggtga agtgtactac tttattcttc cttcatcgag ttttgttctg
    17401 cctcttttgg cgcttcatac atatttactt ggctagtagt cccacgtgtc gacaaattct
    17461 ctacgatttc cttcaagtca agtcctgttg tcgcttttag tgtttcttga gtagaagaaa
    17521 gcaaatttgt ggcataattt gtcactcggt tcgctccact ggtttctcct gaactgcttc
    17581 ctgtatcgac aacagagatt ttttcgatat ttcctagcgg ctgtgctgct tcgcgcatca
    17641 attgcggtag catatcgata accatactca agacagctgc ttcgccatat tccttgaagg
    17701 cattggcgat cttttgtttt gcttcggctt ctgcttttcc ttttgctagt gctgcttcgg
    17761 cttctgcttg tccagctaaa cgtgtcttat tggcttccgc ttctgccagt gcttccacac
    17821 ggaatcgttc tgcttcggct tcagtcactt ctttgacttt ttgagcttgt gcttcttgtt
    17881 ctttagcgta ccgatcggca tctgcttttt tcttcacttc cgaatcatat tgcttttcac
    17941 gacgtgtgat ttctttttct tccagttcga tttgtttttg acgttctacg attttaatct
    18001 gcatttcctg ttcgattact tgttgttgtg ctcgggcact ttctaaattg taggcttggt
    18061 ctgcttttgc tttagcaaca tcttgttctt gtttgtaggt tgctaatttc agttcttttt
    18121 cttttaatga ttctgcgatc tctgtttgtc gctgtaattc agctgcttgt gattcttttt
    18181 ctgcttgagc ttttttgata cgcgtttctt ttagagcttc cgcttctgct atatctgcat
    18241 ctcttttgac ttgggcgatt cttggtttcc ctaaagagtc aagataaccg tttttgtctc
    18301 tgacctcttt gatagtgaaa gaaacgatga tcaaccccat ttttgcaagg tctacagaag
    18361 cgacttcttg tacgctttga ctgaacttat cgcgattttg atagatttct tctacagtca
    18421 tagaaccaag aatcgagcgc aagtgtcctt ctaatacttc tcttgcttca ttttccagtt
    18481 cttctcttgt cttgcctaaa aactgttctg ctgctgtagc aatctcttct acagatgaac
    18541 cgattttgat gatcgaagta ccatcacaca tcaccggtac gccttgttcc gtatacactt
    18601 caggggtgga gacgtccaat ttactcgata atagactgat ccgattcgaa cgttgaaaaa
    18661 ctggcaagac gaatgcgcca cctccacgca caattttgat tttgttattg ctttcatcaa
    18721 tgtgcacatt tttgcttcct aaataacttc cactgatgat caacgcttca tccggttttg
    18781 ctgtctggta cttcgctaca aagacgatca gcaacatcag taaaatcaaa gctgcgattg
    18841 cgatcgcgat aaaaacaact ggtatttcca tttaacacac gctcctttaa gcttttatca
    18901 tgctttcata aggcatgaca taacagattc tttccttgat ctcaatgatc aatactttcg
    18961 ttccccgctc agctgtctgt tcttgggggc tgtaaaaaca agctggacga ctaatggaac
    19021 cggtaacact tttcaactga atttctccca tacctcgtac agggatcggt gtgatcacaa
    19081 cagctacttg tccttccaga gttttttcag aagacgacat cgttgattcg gcatgtttca
    19141 tcggcatcaa tatatagaaa ttaagcaaaa ataccaacac tatcgatatg cctccactag
    19201 caataaataa aaataaagat gattgatctg ttaaacgctc acctagaaaa ccaaataagc
    19261 ttgtgaaagt taaccaaggg ataataagca tcggatcaag cgggccatca aaatcgaata
    19321 catctccaaa aataatcaat aggactgcca atcctgcaca aatcagcaat gtataccaat
    19381 aaatcgtctc aagtgcgatt ccccctatca tatacacgcc tccttccatt tctacgtcta
    19441 tcataccaat gtttgaaaat tctgtatagc ttcttttacc gactcagtgg tgttttttat
    19501 aggaaggttg tgtttatcgt tgacaatttg ttaaattttt gttttcatgt taaaattttc
    19561 acaataaatt ctttcgaggt agcctatgtt tccttttttt aagaaaaaac cattaaagct
    19621 ccagaacacg atcatccttg tcgtttgttt ctccacattc gtttctttga ttgtttctgc
    19681 catattgatc aggaatttta ttctttcgat gaaccgatct agtgtgtcag ataaaatctc
    19741 tagcattgct aaaatcgttg ccaatgataa actcatccaa aaaaagctgc agactggtga
    19801 cccgacacat gcgattcaag gaataacaaa tgaaatcatg attgcatcga atttagattt
    19861 tatcgttgtc atggaccctg aaggcattcg atattctcac gcagacccaa gttacatcgg
    19921 tcttcctttc tcaagtcatg aagatgtgaa aaaagtgatt ggaggacaag aatttttctc
    19981 tgaaaaagaa ggaatgttag ggttcggctt ccgctatttc gtccctattt attatgatca
    20041 agagattatt ggtgcagttt gtgttggctt aacaaacaac acgattgaca atcaagtcaa
    20101 agatggacaa aaaatgattg ttttttcatt gattattgga cttagcgtag gcatcatagg
    20161 cgccgttttt cttgcccaaa aaatgaaaaa agtactgctt ggacttgaac cacaagaaat
    20221 agcagaacag ttaagtgaaa aaacgattat cgaaaatgaa gtagatgaag gaatcttagc
    20281 catctcttct gataaaacta tcctcttgat caatacacct gcaaaaaaca ttttctacca
    20341 aacagagcag ctattggatt tcgaagtagg aaaaaaatca gtgataatct atatgacgtt
    20401 ttgtttaaag agacgattga gcatcaaacg accgttcgga ctcaatccat gtatttaaat
    20461 gggatagaaa ctatttctag cgttacacct ctttttagca atgaccgttt tttaggagca
    20521 gtcgcaactt tacgaaatcg atcagaagta atgaaattaa tggaggagct tggggggacg
    20581 aaacaatata taaactccat gagagctcaa acccatgaat tcatgaataa acttcatgtc
    20641 atttctggtt tgattgattt gaagaagtat gaagaagtca cccattttat ccagcagcta
    20701 aatcaaaatt ataaaaaaga gattggccaa gtctcaagtc tgatcaaagt acctgcaatt
    20761 gccggttttt tattaggcaa aataaacgaa gccaaagaac aatctgttga aatgctgtta
    20821 gaaagcgatt cttttatccc taaaatggag atggatgact ccatccattc acttttgcaa
    20881 gtgttaggga acttacttga taatgcaaaa gaagccgtgc tacaaaaaca tgaaagaaac
    20941 ggacaggtcc ggattcattt aaactatgac acagaagctg aagtattcat cgtgaccgtc
    21001 tgcgatgacg gtgtcgggat atcagaagaa atctcagaaa aaatatttac gctaggtttt
    21061 tcgacgaagg gaatcaatcg tgggtttgga ttgaatctca tccattcaat cgtcaaacgc
    21121 cacggtggat ttttagaatt ttccaaacaa acaaatggcg gtacaatcgt ctatctagaa
    21181 ttaccaaaaa aggaagatca aaatgatgac aaatgtcctg ataatcgaag atgatccaat
    21241 ggttgcagaa ttgaatcgta cttttgtgga aagcatccct aatatgaacg taatcgaaag
    21301 tttcagaaat gtagaagaag gaaaaaaata tttcagacaa aaaaacgcag atctgctgct
    21361 gcttgatgta tacatgcctg gcgaaagtgg tatccatttt ttatcttggc ttagacagca
    21421 aggaaatgat atcccagtga ttttgatcac tgctgcagat gatatcgaaa ccataaagaa
    21481 tgtgatccgc tacggtgcct tagattattt gatcaaaccg ttcactttgc aacgttttca
    21541 gctggcgatc gaaaagtatc ttgctttctc tcagacaatc caaacgactg aaaaagcc
//
LOCUS       DACEZN010000033        21399 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEZN010000033 DACEZN010000000
VERSION     DACEZN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.033"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            125..877
                     /locus_tag="IXH60_000937"
     CDS             125..877
                     /locus_tag="IXH60_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1989894.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELLNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(899..2053)
                     /locus_tag="IXH60_000938"
     CDS             complement(899..2053)
                     /locus_tag="IXH60_000938"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1989895.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            2238..4679
                     /locus_tag="IXH60_000939"
     CDS             2238..4679
                     /locus_tag="IXH60_000939"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1989896.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(5009..5713)
                     /locus_tag="IXH60_000940"
     CDS             complement(5009..5713)
                     /locus_tag="IXH60_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989897.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(5900..6145)
                     /locus_tag="IXH60_000941"
     CDS             complement(5900..6145)
                     /locus_tag="IXH60_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989898.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(6162..7013)
                     /locus_tag="IXH60_000942"
     CDS             complement(6162..7013)
                     /locus_tag="IXH60_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1989899.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            7179..8183
                     /locus_tag="IXH60_000943"
     CDS             7179..8183
                     /locus_tag="IXH60_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1989900.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(8424..8780)
                     /locus_tag="IXH60_000944"
     CDS             complement(8424..8780)
                     /locus_tag="IXH60_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1989901.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            9148..10335
                     /gene="tuf"
                     /locus_tag="IXH60_000945"
     CDS             9148..10335
                     /gene="tuf"
                     /locus_tag="IXH60_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1989902.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(10523..13114)
                     /gene="mprF"
                     /locus_tag="IXH60_000946"
     CDS             complement(10523..13114)
                     /gene="mprF"
                     /locus_tag="IXH60_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1989903.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(13140..13859)
                     /locus_tag="IXH60_000947"
     CDS             complement(13140..13859)
                     /locus_tag="IXH60_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1989904.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            14179..14700
                     /locus_tag="IXH60_000948"
     CDS             14179..14700
                     /locus_tag="IXH60_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1989905.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            14759..15619
                     /locus_tag="IXH60_000949"
     CDS             14759..15619
                     /locus_tag="IXH60_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1989906.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            15726..18119
                     /locus_tag="IXH60_000950"
     CDS             15726..18119
                     /locus_tag="IXH60_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1989907.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            18301..20142
                     /locus_tag="IXH60_000951"
     CDS             18301..20142
                     /locus_tag="IXH60_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1989908.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            20166..21275
                     /gene="rpoD"
                     /locus_tag="IXH60_000952"
     CDS             20166..21275
                     /gene="rpoD"
                     /locus_tag="IXH60_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1989909.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6955 a         3790 c         4435 g         6219 t
ORIGIN      
        1 tttatttatg atagacggaa agccaatcat tttattattc atcaaaagag atagaataga
       61 taaaagagga gaatgattta ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa
      121 tcaaatgaat gaaaaagaat gggatgaatt tgctgaagaa tattatgaaa tccagcaaga
      181 atcgcaagta actattgctg aagatgtaaa aaattatctg aaaacggaga aagtcctgcc
      241 tgctgaaaca gcagcagacg ttgctggagg atctggcaga tttctttcct tagcaaaaga
      301 aattaatcgc tatgatttga tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga
      361 agcagaaaaa aatgagttgc taaatgtttc ctttatcaaa cagtcgttag aagaatttat
      421 ccaatcaaat gagacatatg aaatggtttt tacagcagca aatccagcat tggtacatct
      481 tattcaatta gaaaaattga tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt
      541 ggaatcaatc gatgatgttt tttatcctat cgaaaagcaa ctaggaatga tagaagaaga
      601 tcctaataca tcgccagttg taatggatca atttgaaaca tacttaattg atgaaggatt
      661 caaagtgaag aaaaaagatt ttacttattt tacacaggaa tcaattactg aagaattgat
      721 gaagctatac tatgacgcgt acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact
      781 attcagtgaa aaacgagaaa tccttagtac gactaagctg acctatagac tgctcctttg
      841 gtccgcagat aaaaaaagaa cctttcacga ctcgtgaagg gttcttttta tgattttatt
      901 atattttgta gtaacgaata tcattttcga tctttgtaat cgaaaaaggt acatctgaag
      961 tatattctgc acattctccg tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt
     1021 caacagtgat ccgtgttcga gaatctagca tctcttgatg agattgtgcg aagagatatt
     1081 cttgatagtt ttcttccaga taacctgtaa aaaattcact tactgcacca gaaccgtagc
     1141 tgaatagacc gatgcgatct cctggctgta gtgatttcga attttccagt agagaagtta
     1201 gccccaggta taatgaacca gtgtaaagat taccgattcg tcggctgtaa cggatgcttt
     1261 cttcatagcg agccataaga cgttcctgat tttcttcgtc tgtttggtct aatacgcttt
     1321 gcaatgcctt ttttcccatc ttagtatacg gaatatggaa agcaatcgcc tgataatctt
     1381 cgagtcctcg acccgacaat tctttatgtc gattccaaac tttttggaat gattcgatat
     1441 acgtagaatt agataaagga ccatcaacaa caggaaattc gctataatct ggacgccaga
     1501 aatcatagat atcttctgtc agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt
     1561 tttgagtgat catcatcgca acagcaccga caccttgcgt cacttcacca ccgcttgcca
     1621 agccgtaacg agcaatatca cttgctatga ctagtacttt tcgttctgga tgatttttga
     1681 catattcttt cgccatgtgc aaagctgcgg tgccaccgta gcaagcctct tttacttcaa
     1741 aagaacgagc aaaagattgg attttcagta aatggtggat aattaccgca ctcgcttttg
     1801 aatgatcaat cccggattct gttccgacga tgaccatgtc gattagctca cggtcttttt
     1861 ctgttacgat tttgctggca gcatttgctc ctagtgtaat gatgtcttca tttgcacgat
     1921 tgactgccat ttggtcttga ccaatcccta tatggtattt tgcaggatca tccccacggc
     1981 tttctgccaa ctctgtcata tctaaatata aattaggaat aaaaaaggaa agacgatcaa
     2041 tccctatttt catttcaaca cctctttact tatgataaaa aacttacttt actaaggtat
     2101 cataaaaaga ttaaaagaaa tataaaaaag agaatattta aggattgtta aaatttaatc
     2161 ttagaaaaaa ataactggaa caagagctat ttgtttttac agcaaagaat ggtatatttt
     2221 tgtaggaggt ggcttacttg aaagaagtcg ttatgataga tgctgcaaga acgccgatag
     2281 gaaagtatcg cggaagttta agtccattta ctgcagttga attaggcaca cttgtgacta
     2341 aaggattatt agataaaaca aaactgaaaa aagataaaat cgatcaagta atatttggaa
     2401 atgtcctgca agctggtaat ggtcaaaatg ttgccagaca gattgctttg aacagcggtc
     2461 tccctgtaga tgtcccagca atgacgatca atgaagtttg cggatctggt atgaaagcag
     2521 tgatcttagc acgccagctg atccaattag gtgaagcaga acttgtgata gccggcggaa
     2581 cagagagtat gtctcaagca cctatgctga aacagtatca gtcagaaaca aatgaatatg
     2641 gtgaaccaat ttccagtatg gtcaacgacg gattgactga cgcattttca aatgcacata
     2701 tgggattaac cgcagagaat gttgcaacac aattttctgt gagcagagaa gagcaggatc
     2761 gctatgcctt gtcgtcccag ttgaaagcag cacatgctgt cgaagccggt gtattttctg
     2821 aggagatcat cccagtcaag atttctgatg aagacgtgtt atctgaggat gaagcagttc
     2881 gtggaaatag tacattggaa aaactgggca cgttacgtac agtattctca gaagaaggaa
     2941 ctgtaacagc aggaaatgct tccccgttga atgacggtgc ctctgtggtg atccttgcat
     3001 ccaaagaata cgcagaaaat aataatctgc cttatttagc aaccatcaaa gaagtagcgg
     3061 aggtcggtat tgacccaagt atcatgggaa tcgctccaat caaagccatt caaaaattga
     3121 cagatagatc aggaatgaat ttatcaacaa tcgatctttt tgagatcaat gaagcatttg
     3181 cagcttcttc tatcgtagtt agccaagaac tgcagttaga tgaagaaaag gtcaatatat
     3241 atggaggagc aatcgcatta ggccatccaa ttggtgcaag cggtgcacgt atcttgacta
     3301 cattagctta cggattgctt cgtgaacaga agagatacgg tattgcttca ttatgtattg
     3361 gtggaggttt aggcttggca gtcttgttag aagcgaatat ggagcaaaca cataaggatg
     3421 tgcaaaaaaa aaagttttat cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa
     3481 aaaatgtatt gacacaagaa actgcgttga tttttcagga acaaacactt tctgaagagc
     3541 tctccgatca catgatcgaa aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc
     3601 agaattttca aatcaatggc aagaaaaaat ggattccgat ggcaacagag gaaccatctg
     3661 tcattgcagc tgcaagtaat ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc
     3721 agcggctgat gcgagggcaa atcgttctat caggaaaatc ggagtatcaa gcagtgatca
     3781 atgcagttaa tcatcgaaaa gaagagctga ttctttgtgc aaatgaaagt tatccttcaa
     3841 tcgtgaaacg cgggggcggt gttcaagata tctctactcg ggaattcatg gggagtttcc
     3901 atgcatactt atctattgat tttttagtgg atgtaaaaga tgcaatgggc gctaacatga
     3961 tcaactcgat tcttgagagt gtggcaaaca agttacggga gtggttccct gaagaagaga
     4021 ttctattcag tattttaagt aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa
     4081 tcccattcga acgcttaggg agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc
     4141 aacaggcagg ggaatatgcg aagcttgacc cttaccgtgc agcaacgcac aacaaaggga
     4201 tcatgaatgg tatagaagct gtagtggcag ctacaggaaa tgatacacgt gcggttagcg
     4261 cttcgatcca cgcctacgcg gcaagaaatg gattatacca aggattaaca gactggcaga
     4321 tcaaagggga taaactagtt ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg
     4381 gagcttccaa tatcctgcca aaagcaaaag catctttagc tatgttagat attgattcag
     4441 ccaaggaact agcacaagta attgctgcag tagggttagc tcaaaacctg gctgctttgc
     4501 gtgcattagt tacagaagga atccaaaaag gccacatggg acttcaagct cgttcattag
     4561 cgatctcaat tggggctatt ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag
     4621 cagaaaagat gaaccagcag acagctatac aaatattgga aaaaatccgg gaaaaataaa
     4681 aaagcaatat ctttagaaga ataaagcaag atttacagtt attttattta ctgcagatct
     4741 tgcttttttc tctatcagaa aatatgttta ttattagaat tttttatagt taaggttagg
     4801 catcttaaga aatcaggcaa ctccaagata tcttcaggta gaagagaagt cgcctaacat
     4861 tgtaggatca taggtgattt gttggacagg tcaagctgat atttgagcct gatgttagaa
     4921 ggaatttctt tttacttgct gtagaaatta ttatatgact aaaaaggctg ggattttaaa
     4981 tcccagcctt ttatgacaaa tagaattttt atcgatcagt aatcgctgtc gctgcaaatt
     5041 gttcgatcaa aggaatcaaa gtcaatttga ctttgtattt ccgttcgagt gctttttcga
     5101 taacgaactc attttcagta gtcatagaaa tagttgctaa tttacgaggt tcatcagcat
     5161 ttttttcatt aataactact tcatgttcat tttgttctgt gtaaaattta ggatactcag
     5221 caacggcttt tgaaaaatca gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat
     5281 tttcagttgt gtccgctttt ggtttttccg ttttttcttc tggcacactg actgtttctt
     5341 ctgtaactgg tgtcgcaaca gcttgcgcag ttggttcatc gtttttcaat cgttgatcat
     5401 tccagtaaga cacgatttca ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt
     5461 ctcctgaacg aattctcttt aatacagtat tgaagttgtc atcgattggc acgttgatcc
     5521 gtgtatcttc atttgttttc ccagctaatc cgatcgttaa gatttgacgg tttttgggat
     5581 caagcatgac ttgtaacggc aaaccataac gtcgcaagct ctcttcttct agagaacgga
     5641 ataattgtcc tcttacacca tccaatgaag gacggaattg atttaataat ttttgtttct
     5701 gttttttctt catatgttct ctccttcgct catacccttt cgattataag aaaaaaacag
     5761 ccaaatgaaa aggagtaatg caaaaaaact tgcgtatacg caaattacaa cagaaaaatt
     5821 tgcgtatttt cccgtttttt gaaagaaaaa taaaaaatgg aacggattat gtatatatac
     5881 ataatccgtt ccattctttc tattttttcg gtatcaaggc aaaaacaggt agttcaggtg
     5941 aataaatgac ttcatgttcg tgccagggtt tgaccattag cgaatgatcg aaccgttttt
     6001 tcagttcttc gatgatttga tcatgtgtcc agtttcttgc aggaaaatgt tggattttct
     6061 ctgcatcgat caaaaagaca aaatcggaat gaaaagtctg ggtaacttct tccgagagag
     6121 aattcaagcg ttcgattgga tccataaaat cacctttcga cttaaaattg ggtagtatct
     6181 gggtcttttg ctttgccgac tacaccatct aactgatcga tttgtttcat ttcttcttct
     6241 gttaattcaa aatcaaataa ttcagtattt tctttgattc tgcttggtgt cactgactta
     6301 ggaagaggaa ggaattcgtg ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg
     6361 tcatgtgctt cagcgatttg tttcatttca ggcacatcga agattttacc agtgcctaat
     6421 ggactatatg cttcgaggat catatcatgt ttttttgcat actcaactac agttgtctgt
     6481 aattcgcctg gtgcaaggaa aacttgattg accatcggca taatttttgc tgtttttgct
     6541 agtgtatcta gatggtgggg taagaaattg ctcacaccga ttgctttgat tttacctgct
     6601 tcatacagtt cttcaaatgc tcgccatgta tctgcatttg cttgttccca attatcacga
     6661 aaagcaacag ggtttggcca gtggatcaaa aacagatcta aataatctgt ttggagcttc
     6721 ttcaatgatt cttcaaaaga agacatcaca agttcatagc tgtggtttgc attccataat
     6781 tttgttgtca agaatatctc ttctctagga attccacttt ccttaatcgc ttggccgacg
     6841 ctttcttcat tcttgtatcc ttgggcagta tcgatatggc gataacctgc agccagcgct
     6901 tctttaacag aggaaacagc gacatcccca tcaggtgttt gccatgtgcc gaatcctact
     6961 accgggattt cgtaaccatt tgatagacga taagtgcttg ttaatgaatt catactttca
     7021 gctccttttt tattcgttca actataagtg tagcgcattt ttcgaaaaaa agaatccatt
     7081 ttgtttctac tcttgaaatt tttgtttaaa agaaaatgat tttgattctg gtatttttga
     7141 ttttttttcc gtaaactaag acagaaggga atgagaaaat ggatatctat ttaaaaaaag
     7201 caattctgca tattattgac agagaaactg gttctccagt cttttcacaa aaagagctgg
     7261 atctgacaaa agaatatatt cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg
     7321 ctcaaacaaa aactggacaa ctagcagaag aaagcatgat cgttgaatca cttaaaatcg
     7381 ctcaagaaaa ttttgtggaa gcaagtgaag caatcgttca gcggtggttc gatatctacc
     7441 aagaaagtga agaagcacct agtgcagatg tatttgttgt cttgtatgaa ttagatacag
     7501 tgatgtactt atctttattg aaagtcaatt atcgtgaggc ttacactcac tttgttgaag
     7561 cagacgaagc agggatcgac aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa
     7621 cacaaaaagc agatgaagca ttcgctgtga atttgactga ttggacctac gaattaattg
     7681 aaaaaaaata tgagttttct ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa
     7741 gtgttcctgc accttcttta gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc
     7801 ttggaaaaaa atttgagaca gaagaatttg atatcatggc tgatttgaaa gaagctgtct
     7861 atgatacgat agaagaaaaa ggtcggttgg atcatgagat gattgcggaa aaagtcttca
     7921 aggaaaacat cacggcaaaa atggctttta aagaagaagt acaagagcaa ggatttgtcc
     7981 cagaagcacc tcctgtaaaa gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa
     8041 aattcaagct atcaaacggg atcgaactga tcgtaccagt agatgtatat cgcaacccag
     8101 atttgattga atttgtgaat aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg
     8161 atgaagtatt gaatcgttta taaaaatgac tagaatatta ggtgttcaag taccagtgat
     8221 cagttaaatg aattgtgtca agagaaggat cggaaaagac gaatataaca aaaaaagagc
     8281 gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     8341 cagaagcaac tccttcttat ttcttggagt taaacgcttc tgtcccagcc tctttttatc
     8401 taataaaatt ttaagaccat ttactaatta tcgatttttt ctgccgcatt tttaggcact
     8461 tcagaagcct ttatttcttc ccagcttaat acacctttac gaggattgac tttgagttta
     8521 agatacgcat tcatctttaa tggatgttct ctttcttcgc gaagcgtcac ctgttttttt
     8581 tcaccatctt tattataagc cgcttgcttg taggtataag tcgtgaattt ctctccgtta
     8641 tctgcttggg tagctgacat ttcgccattc gtcgtgattt ttgtataata agcttcgccg
     8701 ccgtaaaaat aatgataagc tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt
     8761 acgccaatta attttttcat gtacttcacc tctttcctta ttattaacga aatagaagat
     8821 ttcgtctatt gaaagttgtt ggcaacagct gaattttaaa cttttcctca ttttgttctt
     8881 attgcttcaa aagaaagtta aggactatct tgaagttttt ctcaaaaaac acattttttt
     8941 atttataaaa ggtacttttt tcgcgtataa gacagacgag tgctcttagt tgactttgac
     9001 caatattgac gtataataag cttagttcga atcttacaaa aagtaagtcg gacgtattct
     9061 ttttgattta gtcaaaaaaa gactataata aaaaggtgag agctgaaatt tttcagtgat
     9121 ctatattatt taggaggttt gtaaattatg gcaaaagaac attatgacag aagtaaacca
     9181 cacgttaaca ttggtacgat tggacacgtt gaccatggga aaactacttt aactgctgcg
     9241 atcactacag tattaggcaa aaaaggccta gcaaatcctc aagactatgc tagtattgat
     9301 gcagctccag aagaacgtga acgtgggatt acgatcaata ccgcacacgt agaatatgaa
     9361 acagaaaaac gccactacgc gcatattgat gcaccaggac acgcggacta tgtgaaaaac
     9421 atgattacag gggcagcaca aatggatggt gcaatcttag ttgtttctgc aactgacggt
     9481 ccgatgcctc aaacacgtga acacatttta ttgtcacgcc aagttggtgt aaaatacctg
     9541 attgttttct tgaacaaagt tgatttagtc gatgatgaag aattgatcga tttggtagaa
     9601 atggaagttc gcgagttatt gagcgaatat ggtttcccag gcgatgacac tcctgtgatc
     9661 aaaggttccg cattaaaagc attgcaaggc gatccagatg ctgaagctgc tattatggaa
     9721 ttgatggata cagtagatga atatatccca acaccagaac gtgatacaga taaaccatta
     9781 ctattgccag tggaagacgt cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt
     9841 attgatcgtg gtgctgttcg tgtcggtgat gaggtagaga tcgtagggat caaacctgaa
     9901 acacaaaaag cagttgtaac aggtgtagaa atgttccgta aaacgttaga ttacggggaa
     9961 gctggggata acgtaggcgt gttgttacgg gggatccaac gtgacgatat cgaacgtgga
    10021 caagtacttg ctaaaccagg ttccattact ccacatacaa aattcaaagc agaagtgtac
    10081 gtgttgacaa aagaagaagg tggacgtcat actccattct tcaacaacta tcgtccacag
    10141 ttctacttcc gcacaactga tgttacagga acaatcacat tgccagaaga tacagaaatg
    10201 gtcatgcctg gtgacaacgt gacgattgat gttgacttga tccatccaat cgctgttgaa
    10261 aacggaacga cattctcaat tcgtgaaggc ggacggacag taggttcagg tatcgttaca
    10321 gagatcgaag cataagaaac gcgactcgaa tattttggta atgaaaaatg tttaaaataa
    10381 aagagcggtc caaacaggat ttctgtttgg accgctcttt gttcttcaat tgtataagaa
    10441 gagattgtag aagcatgaac tttcgtttta cttcagtctc ttttattcat catgagagtg
    10501 ttcatctatc ggtatgcagt ttttaatact ttcttcgtat ccattttttc aaaccactta
    10561 ttctaccttg tgtctgctca actggcgcat tatcaataat cagcaaagca atcataacat
    10621 atgcaatcca actatctcta gaatatagag tataacgtga gacccagttt gtagcgtact
    10681 tgcttttata ttctcttaat ccatggaaag aataaaaatg agaaccaaac tcgtaaacca
    10741 agtatgcaat cctttcctgt aaaaaactct ttctggaagt tccgacattt gaaagagggg
    10801 ccatccctaa attgaaccat tggatatgtt cttctttcat ataggcaaac aaatgcaaga
    10861 acaagaaatc catactgcca gaaggtgctt ttttagaatc atatcgcatc agatcaatcg
    10921 ttcctacttt attgttcgtg taagtaggca tgatcgttgc aaaagcgaca atttcttctt
    10981 gggtattttt gacaactgca ataggcgctc tttgtagata atcttcagag aaaaagccta
    11041 aagaaaaccc tttttccttt cgtgaaccca accaattatc agagatattt ttgaaagtac
    11101 tcatttgctc agtagaaaat ggtggttgta agacatcaaa tgtaaatcct tctcgttcga
    11161 tccgattcaa gacagcacga gtgcccttca tttttttccc tgaagttgta aaactattca
    11221 ggtctacata agcttcttcc cccatcttaa tgaaatcgta gccgaattca tgcaaaatca
    11281 taacaatctc ttcgcttgtt tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt
    11341 cgataaaagc ttcgattgct tctggaaaat cttctttctt accagaagga tcacccatta
    11401 caatacattt attattgaag caagcgaatt ggagaagaac agttggttca ccgtcttttt
    11461 cataggcaaa catccgctta tcttttaaga agatcaattg actatcggag tttcctccat
    11521 aagttgtaag gatcttcagg gccttttctt cattaaaggc ttcgccaatc tgtttctttt
    11581 ctccttgaag gaaatgaaca aatagaacga tcatgaaact aacagcgatg atggccaaca
    11641 atcccgaaaa ccaaatcttt tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc
    11701 gatgagggaa atccggcaga ttgtataccc caataacgat gtaaagcaag gacagtgcac
    11761 caataatgat tccatcgatc gtacgccact cccatgaata gatcaattgt tcccgaaata
    11821 attcgttttt agaagcaaga atgatcagaa gcagaataga caagaaaacc accggtgtga
    11881 aactaaaatc acttaataaa acataaagta aagctagaac aatcaagaag attgtaggca
    11941 aatatgctcg tttcacacgt gctgcgatcc ctctgcccat aatgattaaa agaaatccca
    12001 atagtatact aggaaattgg ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc
    12061 agcgaaactc tgtaaaagct tgcgggatag tggcagaaag tactagcatg attccagaaa
    12121 agtataataa gacaaccacg attttatgcg caatttctgt tgctaattgt ttagggatac
    12181 cagaataacg ctgatcaaaa gtggaaccga tatttttgaa gaaaaagacg attccaatca
    12241 aaaacggaat gatgtagtag aacaggcgat agagcaagat ccacacaacg actatttctc
    12301 ttggcacacc aatagcggac aacccgataa tcatcattac atcgaaactc ccaatttctc
    12361 ctggaatcat agacacgatt cctatcactg aagcagcaat gaataatgga attgtttgcc
    12421 acagatcaat ccggatttcc attaaataac caacagaaag gaaactaaac aggacaccgg
    12481 tccattccaa caatgaaact agcaatagct gtccttgagt tttaagtgag gtaccaccaa
    12541 ggaatccttt ctttttgaaa gtagtaaata aaaagacgac aggaaaatat agaccgccgc
    12601 cgatcaacca tatccaatat tgcttcaaaa aaggagcaac tggcgtgaag actacaagta
    12661 aaaaagaaag cagactgtaa atagaaagtc cagccatcaa aaacaagaaa actttcgata
    12721 atgcttggat cacgtttctg ctctctttct tttgtccata tagttcagag cgaagcccca
    12781 cactaacaaa cccgccgaaa ccggcaatat tgttgatcgt attgatcagc cagcttgtct
    12841 caaacaggta acgaggattc tgcttttgtt caagcatccg attcaaaata atatcataac
    12901 caaccatagg taacacagag ataagaccaa tcaatagcat tagtccgctt ttccatacag
    12961 gtatagtggc aaacgtttct cctaattgct ggatagatag agttttaccg atggacatca
    13021 attcaccgac aataatgaca atgacagaaa tcaaaaagat tgttttgaac agtcctaggt
    13081 ggtttttcag ccattgtatg gtatttttta acaaataaat caccttctat tcctaatagt
    13141 tatgtttcag aaagaaattg aagaacttct cgattgaagc gttcaggatc ttttctagct
    13201 aattcatgtc cttgttcttt cacaagcaca aaggaagctt ttggtatagt tttagctata
    13261 tacaaagaat gttttagctt gattacatct ttttttccca cgatgatcaa agtgggggag
    13321 ttgatttttt ttaaatcatt ttctgtaagc ccgatgtcat gcagcaaaag tttgataact
    13381 aaaagatttt tgcgaagact tgggcgaaat aaagagagta accataccca tgcataatga
    13441 atattactga tgactctggc agaaaaacga acgcctttaa caagcgtgtt tccagaattt
    13501 aaaatcaatc gatgaacttt tttagggaag ctgctggcaa ataccagtgc tagatttgct
    13561 ccatcactaa atccaagaaa atctgcctga tcgatttttt ccagcaacat gatcgtattc
    13621 agatcctcgg ccattaactg aaaattcaac atagatgctt cgtttgtcga acgaccatgt
    13681 cctcggctat ctactagata cacggtatag tagcgttcga gtacagggac ctgttctgaa
    13741 aaaaagcggc cgctgccgtc attgccatgc agtaaaaaga gagggaagcc ttgaccgctc
    13801 ttttcatagt aaatttcgct cccatctggc atttttgcat atttcttttc ttttttcaca
    13861 taatcaccta ctgtgtaaag tataacgatt atatcagtca ttgggcaatt gtttttactg
    13921 aaaaacaacg atcattttcg taagatgtca aaaaatacag gaaaattcat tttttcttaa
    13981 ggcgtacaca gaaatcaagg aagaaatagc caagaagata aagaaatagt aagataaaaa
    14041 aagatgtcaa aataactagg tgatgaaatg aagcgaaaac gattatctat tatccgtatt
    14101 cttttttaca tgatcattct gacggttatt ggtgcggtgt tgtttttatg aggaagaact
    14161 tatcgttcgg taaaacgagt ggaaagctat caaatagaaa taacaacagc tgcaaaaaat
    14221 tatcatatgg aaaactacga gaatttgatc aaggcgataa tattgaccga atcaaaagga
    14281 aaagggacag atctcatgca gagctcggaa agtctgcatg gagaagctga gatcattgat
    14341 aatccagctg acagtattga tcaaggtgtc aaatatttgg cggaaatgat agtagaagca
    14401 aagaaacaag gatgcgattt atggacagct gtccaagcat ataattttgg gaaagattat
    14461 attgcctatg taaaaaatca tggcggagaa aacacagtag aattagcaga agcgtattct
    14521 aaagatgtgc tgagcccact tttaggaaat gaggaaggga aacaataccg atattggcgg
    14581 atccagtcct tgctttacaa tggcggctat ctctatcata atggaggtaa catgttttat
    14641 cctgatattg tcaaaatgaa tgaaaaaata atagagacct atgaaaaagt ctttaagtaa
    14701 aatagcagga aatgttcaat catgaataaa attaaatgaa agaaggagtg cgcaaatagt
    14761 ggaaaaaagg caaaaaatgg aaaatcaaga agtggggtac acaaccaaag actggatgtt
    14821 acggtttgtg aagggaatgt ttatcggttc aggatttatc ttgccaggag tcagcggtgg
    14881 tgcattagca gctgtctttg gcatgtatga gcggctgatt tcttttttag cccaccttac
    14941 aaaaaatttc aaagaaaatg ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg
    15001 catctttcta ctttcttttt tagtcagttt tttattaggt gcgtatgaaa caacgatatt
    15061 atggtttttt gtcggatgta tcgctggaac tgttcctgcg ttatggaaag aagcagggaa
    15121 aaaaggaaga gatacttctg atgtgatcat catggttgtt acttttgttc tggcttattt
    15181 gtttttatta tacggcgctc aattatttga cgggcaagtc gatcagaatt ttggaacatg
    15241 gctgattgcc ggcggattga ttggtttggg tatgatcgtc ccaggattga gcccttctaa
    15301 ttttttggtg tatatgggat tgtataaagc gatggctgac ggatttaaag aagtacgact
    15361 tgaagtgatt gtgccgatcg gaattggtgg gattctttgt gttcttggat tgtcaaaaat
    15421 catggattac attttctcaa aggcatatag caagctattc catttcattt taggtgtagt
    15481 ttttgcttca acgattatga tcattcctac cgattacgcg ggattgggag ttgctggggt
    15541 actagcttgt cctgttcttt ttatagccgg tacagcacta ggttggtgga tgagccgttt
    15601 ggaggaacaa tataagtaaa gatagattga taataaaaac agcacacggg tgattcaaac
    15661 aaaaaggttg gcatcttttt tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa
    15721 acaagttgtt tttaatcttg gaacacgcta gacttaaaca cgtgattatt tttgaaagtt
    15781 ggcggaaaat ggagaaaaag aaaatgaatg gacttacaca gcaagaggta gttcagaaaa
    15841 tggcttctgg ccagcaaaat gactaccagg aagatgcatc aaaaagtaca aaacaaatat
    15901 tcagcgataa tatattgacg ttattcaatt ttctgaactt tggtattggg atttgtctgt
    15961 tatttgtagg tgcgtattcc aacatggcgt atttagcaat tattgtcgtc aatatcgtta
    16021 ttggaattta tcaagaaata catgcaagaa atcttgtacg gaaattatcg attgtttccc
    16081 aatcagaagt actagtgatc cgggacggta aagaacaatc gatttctact aaagagctgg
    16141 tattaggaga tttggtaaaa atcggagcag gagaacagat accagccgat ttacaggtta
    16201 tatctggacg ggcagaagca aatgaagctc ttttgacagg cgaatctgat ttgattgtca
    16261 aagaaactgg agctgaattg ctttctggaa gctttttgac aagcggttct gtcatggctg
    16321 aagttatccg agtaggtgca gataattatg ctgtccgctt gactagcgag gcaaagactc
    16381 acaaagctat tcgttctgaa cttgtagatt cgattcgcaa agtatccaaa ttcactagtt
    16441 tcgtgattat tcctttgggg atcatactat ttttggaagc actgttcatc cgcgatgctt
    16501 ctatcaaaat ttctgttatt gcatctgcag cagctttgtt aggcatgctt ccaaagggtt
    16561 tggtattgct aataagtatt gcattaacga caggggtaac caaactggca aaaaaacgaa
    16621 tccttgttca agacatgtat tcaattgaaa cgctggccca tgtcgataca ttatgcttgg
    16681 ataaaacagg gaccattact gaagggaaaa tgagtatcca gcgggtagaa gcactacatc
    16741 cgctgtacga gaaagccatc ccagaaatta tggggacgta tctggcagaa agcagtgata
    16801 acaacatcac tatgcgggct atgagagatt attttgagct ttctaatcgg tatgatgtga
    16861 caaacaaagt cgcattttct tctgatcgca aatggggcgc gatggaattt ccagaactgg
    16921 gagtcgtgta cgtaggagcg cctgaacgtt tggtggaaga agatcggctt ccggacagtc
    16981 taaaaacagc ccaagcgaat ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc
    17041 aattgaacga agaaaaacca gcatttgttg agccggttgc tctgtttgaa atcgatgatc
    17101 caatccgaaa aaacgctcaa gaaacactag cttatcttca tgaggaaggt gtggatctga
    17161 aagtgatttc tggagacaat ccttttactg tctccaatat cgctagacgt gcaggattac
    17221 cgaattatga tgcgtatgtc gatctttctc aaattactga agaaatggaa gtgcgagaag
    17281 ctgctcatcg ctatacggtt tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa
    17341 atgaattgaa agaaagcggc agaacggttg caatgacagg cgacggtgtg aatgatgttt
    17401 tggcgttaag agaagcagat tgcagcattg ctatggcaga aggtgatgga gcaacacgtc
    17461 agattgcgaa tctggtattg ttggactctg attttactac attgccagaa gtcctgtttg
    17521 aaggacgccg agtggtcaac aatgtgacga aagtctcagg catctttttt atcaaaacga
    17581 tttattcatt tattttatcg attatatgtg ccgttacagc aattggattt ccgtttatcc
    17641 ctatccaaat cacgctgatt gatttagcga ttgaagggta tccttccttc tttttatctt
    17701 ttgaacaaga taaaagaaaa atcacttacc gttatttgcc tacagcactt attaatgctt
    17761 taccaaatgc cttgcttgta gtagtgaata tcattgcggt gtatctactt ggaaaaacat
    17821 tcgcatgggg aaatgctgaa actactactt tgatgtatta cttgctgatt gggatcagtt
    17881 gcatggcagt tgtaaaagct tgttatccct tcaacccatt gcgcatcttc ctagcagtaa
    17941 cgacgatcgt agggatatat gtagctgcaa tgctgttcca tggaattttg gaagtaaact
    18001 tagggttggg agagagcgta ggatacttct ttgtattcat gctcgtaaat attgttcttc
    18061 gagtattgta ctggaaggct gatattcaaa atcagttact gaaaagagta gcaaattgat
    18121 gcttaatgag atagaaatgt gttatacttg aaaaagcaat gaaatatgat acaatcaaac
    18181 atgtataatt gtgtccttta aaatcgcact gatttcagtt agtgcgattt tttaccggtt
    18241 attctcctga aatatcttct gttaaaccca aaagatatca atagagcagg gggtgaaaaa
    18301 atggctcaac ggattcctca agaggttatc gaaacagtac gcagtcaaac gaatatcgtt
    18361 gaggtgattg gacaatatgt ccagttgaaa aagtctggta aaaattactt ggggctgtgt
    18421 ccttttcatg aagaacgtac accgtctttt tctgttgcag aagacaagca gatttttcat
    18481 tgctttggtt gtggtaaagg cggaaacgtg tttacttttc ttcaagaact agaaggtcta
    18541 agttttccag aagcagtggt aaaagtggca gacttcgaac agataccgtt agaagatcaa
    18601 tggcgtcagc gtactatagc tgtccaaaat cctgaatctg ctacggggaa actgattgct
    18661 gctcatgaaa aagcagcgga aatctatcat cacatgctat tgcatacaaa agctggacag
    18721 ccagctttag aatatttgca aagtcgtggg ttgtctttag aattaattga agaattcaac
    18781 ataggttttg cacctaatga gcgttctttt cttcagcaag tattcaagaa tgaatcaatt
    18841 tcaaatgaat tgttagaacg ctctggattg tttgtcacgc atgatgatgg ccgtatgtct
    18901 gatcgttttt atcaaagagt gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt
    18961 ttttcaggac gctggcttgc acctgaaaaa gggcaggaaa aagatcagcc caaatacttg
    19021 aatagtccag aaacagaact gttcaataaa agacaagttt tatttaatct tgataaagca
    19081 agaagcgata tccggaaaaa cggagaaatt ttgttgttcg aagggtttat ggatgtgatt
    19141 gctgcatggc aggctggtat caaaactgga ttagcttcta tggggacaag tctaacagct
    19201 caacagatcc aacaaatgga aaagctgacg aaagaattgg tcttttgcta tgacggagac
    19261 aacgcaggtt ttgaagcaac caatcgaggg attgaattgc ttcggcaaaa cagtcgatta
    19321 gcactttctg tcgtagtcgt tccagaaaag cttgatcctg atgaatatct ccgtaagtat
    19381 ggagaagcat ctttccaaga attagtggct catgggcgcg aaacagtctt tacttttaaa
    19441 tcacgttatt taaaacaagg gaaaaattta gataatgaaa aagacaagat cgaatacctc
    19501 gaagaattac tagttgaact tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta
    19561 tctcaactgt caacagagtt tcagatcggc agagagacga tacaaaaaca actgcgcgat
    19621 cttcgaagga caaatcagca aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt
    19681 acgagacaag aaactgcacg gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag
    19741 attctattat atcgtgcatt taaagaagtt tctgtccgta atattttaaa agaacgagaa
    19801 tttcagttta ttcatgatgc atatgcagaa gtttattttt tattcgatgc atatgtctca
    19861 caacattatg aattcaacct tgcagagttt ttagactttt tgcaagatga aaatttaagg
    19921 cggttggtgg tggaaatcga atatttaccg gtggcagaag aaagttcgcc acgagaaatc
    19981 caagacttgc tagatgtgat cagcaaatct ggcttagctg atgaaataac attaaaaaaa
    20041 caaatgcagc aagaagccag acgaactgga aacaaacagc ttgagcttga attgacgatt
    20101 gaaatcatca atttaacgaa acagttaaaa caagccaaat agctactcaa ggggggcctt
    20161 cttttatggc aaatggaaca gataaaaaaa cgtatgaagc agcagtagca gctttcatca
    20221 aagaaaacaa acccaaacga caagttgtat atgatgattt atccaatcag cttgctacac
    20281 cattcacttt gaatgcagat gaaatggaca aattgatcca aaagattgaa gacgcaggta
    20341 tcagtgtggt ggatgaaaat ggtgatccat caattcacag cttgaagagt gctgagaaaa
    20401 aagctgaaca agcaaaaaca gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc
    20461 cggttcgtat gtatctgaaa gaaattggcc gtgtctccct tttaactgcg gaagaagaag
    20521 tagcgttagc attgaaaatc gaagaaggcg accaagaagc gaaacagcgt ctggcagaag
    20581 caaaccttcg tttagtagta agtatcgcta aacgttatgt aggacgaggc atgcagttcc
    20641 ttgacttgat ccaagaagga aacatgggac tgatgaaggc agttgaaaaa tttgactacc
    20701 gcaaaggatt taagttttca acctatgcta catggtggat tcgtcaagcc atcactcgag
    20761 caatcgctga ccaagcacgt acgatccgta ttccagttca tatggttgaa acaattaata
    20821 aattgatacg tatccaacgt caattgctgc aagaccttgg aagagaacct acgccagaag
    20881 aaatcggtgc ggaaatggat cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc
    20941 aagaacctgt ttcccttgaa acgccaatcg gtgaagaaga tgactctcac ttaggtgatt
    21001 tcatagaaga ccaggaagca acaagtccag cagaacacgc agcttatgag ttactaaaag
    21061 aacaattgga agatgtacta gatacattga ctgatcggga agagaatgtc cttcgcttac
    21121 gttttggttt agatgatggc cggacgagaa cattggaaga agtagggaaa gtatttggtg
    21181 ttactcggga acgtattcgt cagatcgagg ctaaagcatt aagaaaattg cgccatcctt
    21241 ctcgttcaaa acaattaaaa gatttcttag aataagcgtg gactattgac cagaagagaa
    21301 atcagtaaaa ctgtttggtt tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa
    21361 gctttttttt ctataggaag gaaattcatt cattatttt
//
LOCUS       DACEZN010000034        21076 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEZN010000034 DACEZN010000000
VERSION     DACEZN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.034"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            249..935
                     /locus_tag="IXH60_000953"
     CDS             249..935
                     /locus_tag="IXH60_000953"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1989910.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            955..4536
                     /gene="smc"
                     /locus_tag="IXH60_000954"
     CDS             955..4536
                     /gene="smc"
                     /locus_tag="IXH60_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1989911.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            4545..5354
                     /locus_tag="IXH60_000955"
     CDS             4545..5354
                     /locus_tag="IXH60_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1989912.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            5365..6363
                     /gene="ftsY"
                     /locus_tag="IXH60_000956"
     CDS             5365..6363
                     /gene="ftsY"
                     /locus_tag="IXH60_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1989913.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            6550..8817
                     /gene="gshAB"
                     /locus_tag="IXH60_000957"
     CDS             6550..8817
                     /gene="gshAB"
                     /locus_tag="IXH60_000957"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1989914.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(8906..9358)
                     /locus_tag="IXH60_000958"
     CDS             complement(8906..9358)
                     /locus_tag="IXH60_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1989915.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            9508..10458
                     /locus_tag="IXH60_000959"
     CDS             9508..10458
                     /locus_tag="IXH60_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1989916.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            10451..11410
                     /locus_tag="IXH60_000960"
     CDS             10451..11410
                     /locus_tag="IXH60_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1989917.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            11407..12162
                     /locus_tag="IXH60_000961"
     CDS             11407..12162
                     /locus_tag="IXH60_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1989918.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            12185..13138
                     /locus_tag="IXH60_000962"
     CDS             12185..13138
                     /locus_tag="IXH60_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1989919.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(13782..14078)
                     /locus_tag="IXH60_000963"
     CDS             complement(13782..14078)
                     /locus_tag="IXH60_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1989920.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            14433..14789
                     /locus_tag="IXH60_000964"
     CDS             14433..14789
                     /locus_tag="IXH60_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1989921.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            14799..15698
                     /locus_tag="IXH60_000965"
     CDS             14799..15698
                     /locus_tag="IXH60_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ1989922.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            16215..16946
                     /locus_tag="IXH60_000966"
     CDS             16215..16946
                     /locus_tag="IXH60_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1989923.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            17091..19118
                     /locus_tag="IXH60_000967"
     CDS             17091..19118
                     /locus_tag="IXH60_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ1989924.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            19226..20323
                     /locus_tag="IXH60_000968"
     CDS             19226..20323
                     /locus_tag="IXH60_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1989925.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            20630..>21076
                     /locus_tag="IXH60_000969"
     CDS             20630..>21076
                     /locus_tag="IXH60_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1989926.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         7146 a         3576 c         4409 g         5945 t
ORIGIN      
        1 cttctccaat aaacggtgtt caaagtccag tttctcggta ttaagtcaaa tttatgcaaa
       61 aatatggaaa gccattttca caaaattttt tcttcatact cgaaactaaa cgaatttttc
      121 acaacttctc gtcaatcact aggattatta aagctttact taccttttga ccgaaccatt
      181 tcaaatttga caaaaaacca gtataataag gaagtatcga aaaaatatcc ataaaaggaa
      241 tgtggaaaat ggacaaccag ctgacaaaag aactaaaaga aaactataac atcgtttttc
      301 acgatctaaa tttgcttgaa caagctttta cccattcatc ctatgtgaat gagcatcgca
      361 atctgcaatt atcggataat gagcgacttg aatttttagg agatgccgtt ttagaactga
      421 tggtttctca gtatttattc cgtttatatc ctcatttgcc agaaggaaaa ttgacaaaaa
      481 cacgagctgc gatcgttcgc gaagacagct tgtcgaaatt tgccaaagaa tgtcactttg
      541 atcaatatgt tttgttgggc aagggagaag agaattcagg aggacgtacg cgaccagcat
      601 tgctttgcga cctattcgaa gcttttttag gcgcgttata tcttgaccag ggattcgaca
      661 cgacccatgc attcatcgaa aaagtgattt ttcctaaagt caaagcaggt gctttttcac
      721 atgagatgga tcataaaaca aaactacaag aagtcttgca aaaatcaggt gatgtatcaa
      781 tcgaataccg attgatcaat gaagaaggtc cagctcatga acgagtattt tggatcgaag
      841 tttatgtaga tgatcaattg attggcactg gacaagggaa atcgaaaaaa ttagcagaac
      901 aagctgcggc ggaaaatgca ttggcagcac tgtaaaagta agtgagggaa agctttgtat
      961 ttgaaacgaa ttgaaatagc cggttttaaa tcatttgcag acaagacagt cattgatttt
     1021 gaaaatagcg tcacagcagt ggttggacca aatggcagcg gaaaaagcaa tattacagag
     1081 gctatccgct gggtattagg agaacaatct gctaaaagtc tgcgaggcgg aaaaatgccg
     1141 gatatcatct ttgccggttc cgatacccgt aagcagctga atattgcaga agtcacagtg
     1201 attttagaca atagtgatca ttatctgcca ttggagtaca atgagatcag tgtaacccgc
     1261 cgttatcgac ggactgggga aagcgagttt ttcatcaata aacaaagctg cagattaaaa
     1321 gatatccaag aattgttctt agattctgga ttaggaaaag aatcgttctc aatcatttct
     1381 caaggaaaag tagaagcgat tttcagtagt aaaccggaag accgtcgtgg gatctttgaa
     1441 gaagcagctg gtgtcttgaa atacaagcag cggaaaaaga aagcagaaca gaagcttttt
     1501 gaaacagaag acaacctcag ccgcgtccaa gacatcatcc acgaattgga agaacagtta
     1561 actcctttgg ccgcccagag tgaggcagca aaggagtttt tacggctaaa agaaacactg
     1621 actcaaaccg atgtctcttt gatggttgct gaaatcaaga cagcaaaaaa agattgggac
     1681 aacaaacaag cacagcttgc gaaattcaat ctagaattag gcaaactatc tgagtcgatc
     1741 caagaacaag aatccatttt ggcgaagcag cgtaaagaaa atgcgcaagc cgatcgtctg
     1801 atcgaaaaaa atcagcaagt attattagat ctttcggaaa aactgaagca gacagaagga
     1861 caaaaagatg ttttgcagga gcggacaaag catacacaaa aaagctcgca agaatatcaa
     1921 acttcattag cagaagcaca aaaaaaggtt aaacattttg aaaagcttca agaaagcttg
     1981 atgaaagcag cagcagaaaa agaaacagag atccaaaaag ctgaagcaaa tctcataaaa
     2041 acacagcaag aattagaaaa ataccaaaaa tcaacgaaag aattattggc agaattaaga
     2101 gatcaatatg ttgatttgat gcaagaacaa gcagctgtag gaaacgaact gaagtatcta
     2161 gaacgtcaat atatacaaga aacagcaaaa agcaagcaga cgttagccaa gcaatcggaa
     2221 gtagaagcat cagtagaccg attgatctta caaaaagaag aattgacaca aaagcaggcg
     2281 cagctaaaat catctttaac tgaaacaaaa gagaagttag aaatgatcca acaaaacggg
     2341 aaaaagttcc aagaagcatt agcaaaagaa cagcctaaaa tgtatcaatt aatgaatcaa
     2401 gtccagcagc tacgtgctcg taagaaaagc cttcaagaaa tccaagaaaa ctattttggt
     2461 ttttaccaag gggttcgatt ggtcttgcag cataaacagc agctttcggg tattgtcgga
     2521 gcagtggctg aactgatcga tgttcctgca gatttcacgt tagctatcga aacagcttta
     2581 ggtggggcag cccagcatgt catcgttgaa aatgaaaaag atgctcggca agccatcacg
     2641 tatttgaaac agcaaagagg aggaagagca acattccttc cactgacgac aatcaaacca
     2701 agacaattac cggctcatat attgacacaa gcagcagctg tagaagggtt tatagggatt
     2761 gccagtgaac aagtcagtta tcccgatcaa atccaaactg tggttcaaaa cctgctagga
     2821 acgatccttt tagccaaaga cttaactagt gccaatgcaa tcgcgcaaac gatccgctat
     2881 caatatcgag tggtttcctt agaaggtgat gtgatgaatg ccggaggatc catgacaggt
     2941 ggagcgaata aacgaggaaa ccaaggcagt ttgtttgtac aaaatcaaga attgaaacag
     3001 ttgactagtg aatttgaaga agcagataaa caactccaag cacaagaaaa aaaagttcag
     3061 gagctgcaac aagaaacagc tcgtttagcc gaagaacaag aagttttgcg tacacgaggc
     3121 gaacaattac gttttgaaga acaagaagcg acaaatcagc tacaaaacat cataaatgaa
     3181 cttgaacgtt ttgaaaaaga aaagcaaatc tcaacttttg agacacgtga actgcagcag
     3241 tttatcgaag attacgaaaa gcaaacgaac gaattgaaag acaaacaaac agatcttgaa
     3301 tcgcaacgac aaaaaatcga cgaagaaatc aaatcgttaa gtcaagaaag cgaccagatg
     3361 gaagccagaa gagcacaagt acaaagtcaa aaagcacaag aacaagcaga tcttgctgtc
     3421 ttgaaagagc agtttaatca tctgcagatc cagctccgcg gtgcccgagt ccaaaaagca
     3481 gaggcaaccg aacgtcaaga agcgatagaa aagcagttgg cgacattgac agcagacttt
     3541 tctgatcatg aagtcacaga agaaagtttg gaaaaacaaa ttaacgaatt atctgctcag
     3601 cgagaaacat taaaagccga gttggcaaaa gccaaagagc agcgagatag aaggcaaaag
     3661 gagatcgata agcttgaagc agtcttggca gagcgtaatc gtgagcaaaa agcacgactg
     3721 tcggaacaat ccaaattaga agtccaaaaa gaccgagctg aaatgatttt agataatcat
     3781 ttgacctatc tgcagtcaga atatcagctg acatttgaga aagcttccca agactatcaa
     3841 gaaacaaccg atatcgaaga cagtagaaca aaggtttcat ccttaaaaga acagattgaa
     3901 aaactgggac cagtaaactt aaattcaatc gaacaatacg aacaagtcag tgaaagacat
     3961 acattcttag caacacagcg agatgatctg ttagcagcga aaaaccaatt gtttgaaacg
     4021 atggatgaga tggatgatga agtgcggaca agattcaaag aagtatttga agctatccgt
     4081 caagaattca aaattgtctt tccaaatatg tttggcggcg gtaaagctga attagtactg
     4141 acggaaccaa ctgatttgct gaaaacaggt attgagattg aagtacaacc tcctggcaaa
     4201 aaactgcaaa gtctaagttt gctttctgga ggagaacgag cacttacagc catcgcgttg
     4261 ctcttttcca ttatccgtgt ctgtcctgtt ccgttttgta tcttagatga ggtagaagca
     4321 gcgttagatg aagccaacgt gaaacgtttt ggtcgttact tgagcgattt ccaagatgac
     4381 acacaattta tcgtagtcac acaccgcaaa ggaacgatgg tcgcagcaga tgtactgtac
     4441 ggagtgacga tgcaggaatc cggtgtatca aaaatcgttt ctgtacggat ggaagatatc
     4501 aatgaagagg cgaaaatcaa cgaaacgaaa gtgtaggaac gaaaatgatc aaattagtag
     4561 caatcgattt ggacggtaca ttattaaatg aacaaaagct gatttcagat gagaataaac
     4621 aagcattagc acaagcaaaa cagcaagggg tcaagatcgt tctatgtaca ggacgcccct
     4681 tagctgcgat ggcgcattat ctgcaagaat taggactggt agatgaaggt gactacagca
     4741 tcactttcaa cggcggtttg gtacaaaaaa atgatactgg tgaaatcatg gaaaagaaag
     4801 tgatggaagt atcagatatc caacgcttat acaaactggc tcagcaattg gatctgccac
     4861 tagatgtttt gtctgacaat gtggttttac agctgcctag tgccccaagg aagcaatcct
     4921 tgtacaatgt gttgaataag ctgctgcaat tccaaccagc ggatataagc gatattacag
     4981 atgaatttgt gcttaataag gcagtaatcg cttatgacca agaagaatta gatcctaaaa
     5041 tcaaagaaat accagcagaa ttccatgaaa tgtatgagat catcaaaaca agaagtatgc
     5101 ttttggaatt tatgccaaaa ggtgttacaa aagcttatgg tatttcttta ttggcaaaag
     5161 atcttggttt gaaacaagaa gaaatcatgg cgatcggtga tgaggaaaat gatttaccga
     5221 tgatccaata tgctggacta ggcgtagcga tggagaatgc tgtgcctttc gtcaaagaag
     5281 cagcagattt cgtgacagcc agcaatgaag aaaatggcgt agccaaagtg atccaaaaat
     5341 ttgtattaga gtagggggag tcaaatggga ttttttgata aaatcaagcg tgcttttact
     5401 ggcgaagaga aaacagaaga agttgaagta caggagaagt acgacaaggg tttagaaaag
     5461 acgcgtaaaa catttggtga acggatgaat gagttgtttg ccaattttcg aacagtcgat
     5521 gaagactttt ttgaagaatt agaagaaaca cttatcggtg cagatgtagg ttttgaaaca
     5581 gcgatacgaa ttaccgaagc attacgtcaa gaagtgaaac tcagaaatgc gaaaaaatcg
     5641 gcagaagtcc aaaatgcgat catcgagaaa cttgtggatc tctatgaagc ggaaggaatc
     5701 aatgaagtaa atgaactaaa tatccaagaa gacggactaa cagttatttt gtttgtcggt
     5761 gttaatggtg tagggaaaac aacaagtatt ggtaaacttg cccatcagtt caaaaacgaa
     5821 ggtaaaaaag tattgatggc agcagcagat accttccgcg ctggagcaat cgaccagtta
     5881 gttgtctggg gcgaacgcgc tggtgttgaa gttgttcgtg gcaatgcagg aggcgatcca
     5941 gcagctgtcg tattcgatgc tttggaaaga gcaaagacag agcaggcaga tgttttacta
     6001 gttgatactg caggccgact gcaaaataaa gtgaacttga tgaacgaatt agaaaaaata
     6061 aaacgagtaa tccagcggga attaccagaa gcaccccaag aaacacttct tgtattagat
     6121 gcaaccactg gtcaaaacgc gatggttcaa gcaaaacaat tcaaagaaac aacagatgtg
     6181 accggtcttg ttttgacaaa attagatggt acagcaaagg gcggtatcgt acttgctatc
     6241 cgtaatgaat tgcatttacc agtgaaattg gtcggtcttg gtgaaggaat cgatgacttg
     6301 gagccgtttg atccaaatga ttttgtcgtt ggattgttca aaggattact aaaagaagaa
     6361 taacccatgt ctaaaaagca ggagctgtga caaaagttgc agctccttgt ttgaatgatt
     6421 gtttttaaca tagctgtgtt tttgatgcct tcatttttat acttatttgt aagcggttct
     6481 caaacaattt cgttcttttt cttagacttt ttgattataa ttagattcat atgaattgga
     6541 ggtcacttaa tgatgaattt taagcaatta ttattgcatg tcaatgcgcg cccttttatc
     6601 gaccaagctc gatttggtat agaacgggaa ggacagcggg ttgatcttgc aggaaatcta
     6661 gcaaaaaccg atcatccagc aatctttggc gatcgatcct atcaccccta tatccaaaca
     6721 gattttagcg aaacacaaac agagatgatc acccctgtta ccgattctat tcccgaatta
     6781 tttcagtatc tcgctgctgt ttatgatgtg actgctcgtt ctataccgaa agaagagatg
     6841 atctggccat taagtatgcc acctgcctta ccggaaaaag acgaagagat cattattgca
     6901 aaattaaaaa atttcgaaga tgtcttgtat cgacgttatt tagcaaaaga atacgggaag
     6961 cgaaaacaga tggtaagcgg tattcatttc aattttgaat ttggcgatga gttgctaaga
     7021 acattgttca gccatcagga agaatttcaa gatttttccg aattcaaaac agaactttat
     7081 ttgaaaacag cgagaaactt tatgcgctat cgctggatga tcacttattt attcggtgct
     7141 tcaccaatga gtgagaagaa ctacttttta gatgaatcac atccgcaaga acctgttcgc
     7201 agtattcgca acagtgcgct gggctatacg aatcatccaa atgtgaaagt ttcctatgct
     7261 tctatgaaac agtatttagc agatatcgag cgaatgatcg aagaagggaa actttcggag
     7321 gagaaagaat tttatacacc gcttcgtttc cgaggcggaa agaaagtcgc agatttagca
     7381 acaacaggtg ttcgttatat tgaattgcgg aatatcgatt tgaatcctta tgcaagattg
     7441 ggtatcaatc cagagcaagt tcggttctta caattattcc tgatgtacat gttatggaca
     7501 gaagaaaaag aagactgtga ccaatgggta gcagaaggta cgactcgaaa taacaaagta
     7561 gcactagaac aaccatcaga tcaaacagaa tttcatcaag aaggtagaga aattcttgaa
     7621 ggaatgaagc aaatgctggt tgaattggat tggttggatt ctctttattt agtagaggaa
     7681 gcattgactc aaatggatca tcctgaacaa acgttagcag cgaaactcta tcaagaagca
     7741 cagctatcga gtcagcaaga agttgctgtg gcattgggac atcaatatta taaagaaagc
     7801 catgaacgtc cctatcagtt ggccggtttt cgcgaaatgg aactttctac tcagatattt
     7861 atgttcgatg ccatccaaaa aggtgtccaa gtcaaagtat tagatgagtc ggatcaattt
     7921 ttgcgtctgc aattccaaga ccatgtagaa tatgtgaaaa atgcaaacat gacaagtaag
     7981 gacagctata tcgtcccact tatcatggaa aacaaaacag taacgaaaaa agtcttaaaa
     8041 gaagctgggt tccgggtacc aggaggcgca gaattttcat ccatggaaga agcggtaaaa
     8101 gcttatccga gatttgccga gcaggcattt gtgatcaaac ctaaatcaac caattacggg
     8161 ttaggtatca cgattttcaa ggaaggagct agtctggaag attatcaagc aggtttagcc
     8221 attgcttttc gtgaagacag ttcggtttta gtagaagaat ttatgccggg aacggaatat
     8281 cgtttctttg tgatcgacgg agaagtccaa gccatcatgt tgcgagtccc tgctaatgtt
     8341 attggtgaca gtatccgaac agtgaaagaa cttgttgaag aaaaaaatag tgaccctttg
     8401 cgtggaacga atcatcgtgc acctttagaa ttgatccaat taggtgaatt agaacaattg
     8461 atgttgaaag aacaagggtt gacgatcgaa tcagttcccc aagccaatca aattgtttat
     8521 ctaagggaga actcaaatat cagtacaggt ggtgattcga tcgatatgac tgatgaattt
     8581 tctgaagctt acaaaaaaat cgccgtttct gcggtagaag cactaggagc taagattagt
     8641 ggtatcgatt tgattatacc agataaagaa atcgatccta caactgataa aaaagcatat
     8701 gggatcatcg aagcaaattt caatccagcc atgcatatgc atgtttatcc ttttgcagga
     8761 aaaggcagaa gattgacgat gaatgtattg aaattgttat atcctgaagt attttaaaag
     8821 aaaaagcttt tgacagattg tcctatttga tgtcaatctg atcaaaagct ttttttgcat
     8881 gtaaaaattt atgtgttttt tcgttttaaa ataaacgatc ccagaagctt tttttctttt
     8941 ttggtttttc tttttcagct ggcgaagagt tttcttctgg gtattttttt tcgatatcaa
     9001 tcacaggttc atttactgct tcttttgctg ttaagagcaa tccgaattcc tcaggatttg
     9061 cagtgacgta atcgttgact acagtgaaac ggctattgtt tttcgttgct agctggatat
     9121 attggttttg gacagaaagg ggtagagccc cattcaagag tatggaagca tctggatgtt
     9181 ttttgagttc ttttaaaaag ttttctttgt tttttggttg gatcatttca gcaacagtca
     9241 tactaagata gcagcgttcc ctgaatgtcc ctaaatattt atgctgttct tcagggttca
     9301 ctaaaggtgt accataaagt cccttatcca aatggctttt tacatcatct tgtgccataa
     9361 aaaataccct ccttcttctc tttgtctttc ttacgattat agcatattcc caataatacc
     9421 taaaacatat gaaaaagcgg ttgcttttgt tcaacgattc acttaaaata agaatgaaaa
     9481 tcattctcaa ttagaaaggt ggattggatg cgctacggac gactagtagc attattagtt
     9541 attttaagta gtatttcatt gttcatcgga gtatcgaatg tacaattgtc agaactgctc
     9601 cagttagatg ggcaaagctt acaaatcttg ctagtgactc gtttgccacg aacgatttgt
     9661 ttgatccttg tgggagcgac cagtagtatt tgtgggttga tcatgcaaca tttgacccaa
     9721 aataaatttg tttcaccaac gactgcgggc acgatggata gtgcgcgttt aggtatttta
     9781 gtggctatga ttttcctacc tggtgcatcc ttgattgtcc gatccttgac agcattttgc
     9841 tttgcttttg caggaacatt gcttttttta tcccttacgc gattgttcct ccagaaaaat
     9901 caattgatgc ttccgttgat tggagtcatg ttagggaata tcattggttc agttgctact
     9961 ttttttgctt atcaattcca gttggtacaa aatatgactt catggcttca agggaatttt
    10021 gcgactgtga tacgaggaaa ttatgagtta ttgtatgctg ttgttccatt gttgctcttg
    10081 gtttggctat ttgcttaccg atttacgatc attggtctgg gcgaatcgtt tgcagtaaat
    10141 ctgggaatag attaccagaa gatgcagttt ttaggcattg gcttagtatc attggctagt
    10201 gcagtcatgt tgatcatggt aggaagcatt ccttttatcg gagtgatcat tccgaatctt
    10261 gtctctagaa tatatggaga tcaagtgcac aaaacaattg gcgtaactgc tttagcaggt
    10321 agtttgttct tagtattttg cgatcttgtc gctcgcgtgt tgatttttcc ttatgaaata
    10381 ccagtcagtg tgatcgttgg aatcatcggt ggggctatct tcctttatct tctgataagg
    10441 ggacgacgat atgcttaaaa aaaagccatc tatttttatt ggtatgcttg ttcttgtttt
    10501 atttgttaca ggaatgtact tgacgatcca tacatatgga aactggcatt ttgcatgtgc
    10561 attgaggagt aagaaaattt ttgcatttat ccttgtctcg ctagctgtca gcagtgccac
    10621 aatcagcttt cagacaatta cgcataacca gtttttgact ccttcagtat tgggacttga
    10681 gtcgttatat gtccttgtcc aaacggtcct tttcttcttt atcggaggag tgacgatgct
    10741 tcagcagaca ggtacagttt ttttcttatt gaacgttggg ctgatggtcg ggctgagtct
    10801 attgttattt ttctttcttt tgaaaaaaat gagagcgaat ctttttttat tactgatgtc
    10861 agggatgatt ttaggtacgc tttttggtag tgtcagtacc tttttacaag tcatcatgga
    10921 tccgaatgaa tatgatttgc ttcaaggcag attatttgca agctttggca atatccagag
    10981 tcagtatctt ttgcctgctt ctttgatcat tatcggctgc tggtacctgt tatttcgata
    11041 tcgacatgtg ctagacgttt tccatctcgg aaatgaacaa gcagtaaacc tcgggataga
    11101 cacagatcgt tttgaatttt tgattttatg ttgtgtcagt cttttgacag ggacatcaac
    11161 ggctcttgtg ggtccagtta cttttttagg gtttattgtc gcaaatatca gttatcgtgt
    11221 cttttctact tatcgtcatg gtcagctatt tatcggaagc tttctggtag ctatgctgtt
    11281 tctgacagga ggacagctcg cagtagagca gattttcggc tggaatacaa cagtgagtgt
    11341 cgtcgttgaa tttgtcggag gaatttattt tgtcacaaaa ttgattcgcg aaaggaagag
    11401 tatcctatga ttgatttaca aaaggtcaat aaactgtacc atggaaaatc agtggttaaa
    11461 gatgtacagt taacgattcc tgaaaagaaa ttgactgctt ttatcggtcc aaatggtgct
    11521 ggaaagtcaa cggtgttgtc gatgatcagt cgtttgatac caaaagatac aggtgaaatc
    11581 tatttagacc ataacgaagt aaaggcttgg aaatccaatg aattagccaa aaaattatcg
    11641 atcctacgac aatcgaatga aataaatgtc caaatcaccg tgaaagaatt agtagaattt
    11701 ggacgatttc cttatacgaa aggaaaattg acgacaaaag acgaggaaat cgttcaggaa
    11761 tcactggaac accttgggtt agaagaaata gcggatcaat cgattgacac tctttcaggc
    11821 ggtcaactcc aacgtgcgta tattgcaatg gtgctagcgc aagacacgga ttatatttta
    11881 ctggatgaac ctttgaacaa tctagatatg aattttgcgg ttcaaatcat gcagatttta
    11941 aaaaatttag tacaagaaat cgggaaaaca attgttattg ttcttcatga tatcaacttt
    12001 gctgcaagtt atgcagacga aattgttgca atgaaagatg gacagttatt tacgcaagga
    12061 acgacccaag aaatgataaa aaaagatgta ttggatgaat tatacgaaat ggacatacgg
    12121 atttgtgaga ttgaaggccg cagattctgt ctttatttct aaaactaaca ttaggagaga
    12181 agagatgaaa aaattagtag taggtatctt gattctagca tcatttggtt tagcagcatg
    12241 tggaaatagc aacaacacca gtcaggcaga cacaaaaaaa agcagcacac aaactgaaac
    12301 tacattgacg ataacagata gtaatggaga ccaaatcgag gtgccaaata atccagaaaa
    12361 agtcgtcgta ttcgacaatg gttctttaga tacgatggat gctcttggtg ttggagataa
    12421 ggtagttgga gcagctacaa gcagccttcc tgagtatctt tcatcctata aaaaagtaga
    12481 atcagcaggt ggaataaaag aaccagactt agaaaaaatt aatcagcttc agccagatct
    12541 aatcatcatt tctggccgac aaagagattt tcaaaaagat ctaagtcaaa ttgctccgac
    12601 cattttcctt tctttagatg cgaaaaatcc atgggaatct ttccagcaaa atgtcactgc
    12661 tttaggggag atctttggta agcaagaaga agctaaaact caattagagg aattatctag
    12721 tgcaatcgat cagacgaaaa agaaagcaga agctactgac aaaaaagcac tagtcacact
    12781 tgtgaatgaa gggcaattat cagcctacgg atctggttct agattcggtt tcatccatga
    12841 tttatttggc tttgaacaag cagatgatca aatcgaagca tccactcacg gtcaaagtgt
    12901 ttcttatgaa tatgtgctgg aaaaaaatcc ggatatctta tttgtagtag acagaacaaa
    12961 agctattggt ggagacgaca gcaaagatga tatttctgcc aatgaactgg tagcacaaac
    13021 gaatgctggt aaaaatcagc agatcatctc ccttgaacca gatgtctggt atttaagcgg
    13081 gggcggttta gaatcaatga aactgatgat cgaagatgtg aatcaagcat ttaaatagca
    13141 taaataacag aggattaaac tctcatacta tgtaacaata atgtttctta cattcttaaa
    13201 tagcaagagc ctatcctcaa agaagttatc aaaaaccatc cgtaaaatcg tattgatgat
    13261 tttacggatg gtttttattt atttgagaaa tcttttttca acgtgaattc attatctaca
    13321 tcatacgggt tgattctaga atggggagtg gcaaaaaata gtgtgatcca ttttttcagc
    13381 atcaagatcg cctcctttga tttgaatcaa aggtacaaag cgaaacttag ataaaactta
    13441 aatttagata tttttatttt gtaccgatct cttctttaaa cgatgttcca tctgctgctt
    13501 ctcggcaata agtccactag actagaaaat atgagaagct attttctgtc cagcgttctt
    13561 attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acgatgttcc atctgctgct
    13621 tctcggcaat aagcccacta gactagaaaa tatgagaagc tattttctgt ccagcgttct
    13681 tattactcaa agcaaatcgc agttgtcaca atctcttcta aacaatgttc aatcagctga
    13741 ccttcggtat tagctcaaag cactgatttc acaacctcta tttaggcatg tgtctcagca
    13801 agcggttgat agttgatgat ctgcattttt cgataaaaat cggcgggaag cttactccac
    13861 cgttttaata attgatagaa ataactctct tctgctttga ttttatactc gcttaatatc
    13921 aatccaaaac tattcgtttt ttttgaagct aattttttat aaatatccaa tgctttggtt
    13981 ttatcttcat tgacaaagag tgtttttact tcaatataag aaccctcgat attttgtgtt
    14041 tcttcgataa aatagatgtt ttttatatat tccttcatag tgattcctcc aaagaagtct
    14101 tttatttgat aagtttactc taccgaagag ttgttaataa tccgtgaaca aaaagtaatt
    14161 ctttttccaa tagttgttaa aggtttttta ttttttatag tattttttgt tatttaagaa
    14221 aggaataagg ttaaacaaat aacaggttgc tttttgggtc tttttccgtt caagaaactg
    14281 aacgaagaaa agggaaagtt gtctcttttg tgataagaga aatcataata aacgaatgac
    14341 ttactttcca ataaattgag aacagtcaga accagacaaa ttagttgcat ctgatgaata
    14401 aagtgattat tctatgacat gaggtgatac agatgaaatt agaagtgaca aaagaagcac
    14461 aagaagtatt gaagaataaa ttagaagcag acgatcaact tttattagat atcgaaaacg
    14521 gtgacggtcc atttgcagaa agccaagtca gttgccaatt ggatacggca tttcgattga
    14581 tcattgtcaa aaaagataca caaacagatc taagtcttta ttctgtagtg gctgatacac
    14641 cagttggacc aatcaaaatc aaagacagtg caatacttta catggatgat ccatccacac
    14701 ttgctttaga accaacttat aatagtttac aattaaaagg accaagcggc ttgagaaaag
    14761 gcaacttgca agttgttcga aaagactaga gggataaaat gggaaaattg gcgatcatca
    14821 gcgacctaca tgtggacatc aataaattcg gcgaatcaga gctgatgcag ctttggcaag
    14881 ttttgcaaaa gcacaagatc actcggcttc atctagccgg agatacggct aataaagtag
    14941 accgttgcct agctgttgtc gaatttttcg tcaataaagg attatctacg acatttcatt
    15001 tcggcaatca cgagttggca gatgtgccca atgaacagat gatggaccat tttccagacc
    15061 cacatttttt aaatgaacgg tatattgctt taaatgagca gaccgttctt ttagggatga
    15121 atggctggta cgactatcag ttttccgaat tgaaagatta cgaggcaatc gtacgaatga
    15181 agcgtttgta ttggtatgat cggttcatcc agcgtgggaa aacagatctc gaggtcaatg
    15241 cagaaatgct tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg
    15301 tgattttatc cactcatttc gttcctaaac gggcatttat catgtatcaa tctgctcctt
    15361 atgagcgttg gaacaaatta aatgcatttt taggatcaga atcattcgga gaattacttg
    15421 accgttattc aaatatcaaa caagtagttt ttggccatac gcatcgtcgc tttgaagata
    15481 aatccatcgg tcacacgatt tacagctgca gaccttttgg ttattattat gaatggcagc
    15541 tgacccgaga ttttgtcttg aaagaacagt tgctagacag ctatgatcct atgaaattac
    15601 gtgtcctctt gcgtgcccat cacccagctt ttcaggccta tcaaacacaa catttgcaag
    15661 aggaatttga acaagcgatg acaatcgttc cgtattgaga cagcattagt aatgtgtaaa
    15721 agaaaatacc ttgtaaaaca taaggataag taagagcagt agatccattt atcgtggata
    15781 tgctgctttt tttgcggaaa ataatcagcg aatgtttatt tgatcggatg atttgtaact
    15841 cgcagagtac tagatgcaag cgtgtattct ttttttgaac gataatcaca aaaaaaaata
    15901 taatatacat tttttattta attataatta atattgattg aagtctaggc agtcaagtca
    15961 cattttgata gataggaggg ggacttacga attattttaa aggctatgac agctgtattt
    16021 ctagattgtg tttgcatcaa gttgcaataa aaactagaaa tcaaatcggg ctgataatca
    16081 tttataacta atcgatacat aggaccaaat ataggtctat gttcttttat ttcataagta
    16141 tcgtaaaaca gaattttgct atggattaat ccaatcgact gatatgttta gtaaataaag
    16201 aaagaggaat gtcaatgatg aaaaaagaaa tcgtattaac agcagcaatg atgttattca
    16261 gcatggtagc aagtacgact tttgtttctg caactgaagt atacccaaag gaatataaca
    16321 cagaaggaac gattacattt gaagcaggag atgagggtgt aactcctcct gttgatccag
    16381 agaatcctga tcccaataaa cctgtcgatc catcagatcc tccaagccct ggaacaggag
    16441 gggcattatc gattgattat ggatcaaaat tcaaatttgg gactcaaaaa atttctactg
    16501 cggataaaac gtattatgca gctgctgatg tgatgaacga tgggtctaga aaaccgacat
    16561 atgtccaagt gacagatagg agaagtacct tatctgggtg gaaattaagt gtctctcaac
    16621 cagaacagtt taaaactgcc agtggggatg aactagttgg agcacagctg aaatttacga
    16681 aagggcaggc tgtttctttg gttgatccaa catatacgcc gcaaacggtg aattctgaat
    16741 taacgttgac accgggagga aacaatacgt tagcaattaa tgctaaatca ggaacaggtg
    16801 tggggacttg ggtctatcga tttggtgcaa atgaaaatga aaatcaagat gcggtacagc
    16861 tttcggtacc cggtaaatct gtcaagttag cgcaacaata ttccactaaa ttagtttgga
    16921 cattagaaga tacacctaac aactagttta ctgaaaagaa ctgttaaaaa taaatacgta
    16981 gtcatattgt cttgcgaggt aatcgagcaa aaattgtcaa aggctaggct aaatttgttt
    17041 ggatacgaat agttcatcta ggaatttaac gatcaggagg aaaagatata ttgaaaaaga
    17101 ggaggatcgt gctcatttta agcagtctct tagtgactaa tatctttccg atcatagggt
    17161 actctttgga attgaatcag ttggagcatg gaaatagaag agacgaagaa agcaaactca
    17221 cttcgacatt cgacagttta atagaatcca accagacaca agaaagcagt gaggatttaa
    17281 gtatttcgag tagtggagag accactgaca acgtagaagc tgagggttcg gacaaacaag
    17341 gactagaaga tgcaccaatt tctgcggttg agctgcctga cgatcccaat attatttcta
    17401 tcgataaaat ttttcaaaca cctatcggta ctagcacgtc catattggaa aatgggaagt
    17461 tactgcagtt gaacccagca gaaaggtctc aaagaggagc tatttggtca aataagaaga
    17521 ttagtttact ctcggatttt acatttaaat cttatttata cttaggaaat caacgagata
    17581 gagcgggaga tggcatgacc tttacattga ctggcgatcc tagaatggct agtaatccca
    17641 gtcaagttat tggatcgcca ggtatgggaa ttggctcata ttctactcgt tctggccaac
    17701 cttacattgc gaatgcgctc tcgattgagt ttgatacgta taaaaatagt ggttcttctg
    17761 atcgaatgga tcgagagata actgctgata atagagggca tggacatgtc gcttttgtca
    17821 cgcctaaacc aaataataat aattattctg gagagcatac tggcgtgacg gtagctccta
    17881 catttctttc taatggcaca tggcgcttgt taataattaa atgggacgcg gagcaaagaa
    17941 gtcttactta tgatttggaa ggtgtcggtc actcttctcg gattatacct gatctcatta
    18001 ggcagtttgg ttctacagaa gtaaattggg ggtttacttc ttcaacaggt actttttttc
    18061 aagaaaatgc tgtcgctgtc acgcgacttc catcgaatgt cacatcaaca gctgatgtaa
    18121 aagtcaacga tagtgattat ggggccatcg ctgaagctgg aggaaacgat aagataacta
    18181 tcagaaatac attgaaggtc gagggggatt ttcttgaata taagaaaatg cagactagta
    18241 tcgacctccc acaagaatta actattcccg agcatatctt gatcaatgga aatcaaattg
    18301 atgccaaaga tatcttgata accggaaatc gagtcacgct aaatctgaca gattatttta
    18361 ttgctggaga tacgttaata atcgaattgg agacaacttt gcaggaagta attcctgaaa
    18421 aagtactcac tatgcatttt gaataccttg aaaacgggaa tttgattgag caatcgaatg
    18481 aaataaacct aaagatcgca aagctgaagg agatagatat ccatgtttat tataaagata
    18541 aagacagtca gattgatatt gctgaaatga aaacaatcac tgggaaaata ggagagaagt
    18601 ataaagaaac tcctattgat attgaagggt atgtttttga ctcggattca gcgaatacag
    18661 aaggcatctt ctccgaaaat acagaggata tctattttta ttacagaaaa ggcaaacttt
    18721 atctctcaac tgctcctcaa tatgtcaatt ttggaaaaca taaaatatcc agtagaccat
    18781 taagtgaatt cggacagttt tcaggtgggt tgaaaattat agatgaacgg gcttttggaa
    18841 gttggaaatt acaattgaaa caagagaaag ttcttacaaa tggagatttt gagcttccag
    18901 aagctctttc ctttgttacc tctgaaaata caacggttat agggacatcc gctgttacga
    18961 ttttcgaaag tgatcaaaag ggtgaaagcg atttgtcctc tcttttagat tctacagctc
    19021 atcgtggaat cagaatagat attcctgtag aaaaccaaag aatcggaacc tttgagggga
    19081 aactatcttg gatacttgca gatgtcccag gaaattaaca ggaagagaag aatataacaa
    19141 aattattgag tttcaagaag ggcacaggta ccataggctc ttgtgtccca cagagacaag
    19201 attttgttat agtcttctct tacatatgat taaaggagcg attttcttga gaccaaaaca
    19261 acaattattt ataacactcc tagttatttt aggggcactt tttaacaata tatcagtgtc
    19321 agctagtgca tttaattttt cagtgacgcc gataacttca gagaatcaaa tcgacaagag
    19381 aaaaacttac ttcgatcttc aacttgatcc ggatcaggaa gtagaagtaa aagcggaatt
    19441 gagaaatgat acagaaaaag aagtgaaaat cgatatttct gtgaatagcg caaccaccaa
    19501 ttcaaatgtg atggttgaat acggaaaaaa cgagattgaa aaagacatga gcctgatctt
    19561 tgatctggta gattatgtga gctatccaca gatagtgacg ctaaaaccta aaagtgtcca
    19621 aaccgttgct tttcatgtca ggatgccgaa tgagcgattt gatggtgtat tggctggtgg
    19681 gatcacattc caagaaatcc agacggaaaa ggaccaaaca gaaactaagg atcaaagctt
    19741 atcgattgag aatgaataca cctatattat tgctttgttg atgcaacaga cactgaacga
    19801 agttgctccg aaccttactc ttcatgaagt taaaccagat caaatcaatg caagaaatgt
    19861 catcttagca aacgttcaaa atgatcaaaa aacctatatc aatcaagtag ttattgaaac
    19921 aaaaattaca aaaaaaggac attctgaagt tttatatcaa gaggaaaaag aaggattgca
    19981 gattgcgccg aatacaaatt tttcttttcc aactgcttta aatggtcaac cgctaacccc
    20041 aggtgaatat caccttacaa tgactgtttt aggaaatgaa aatgaaaatg gaaaattttc
    20101 gagaaaaaaa ggaaatacta cgatcaacta taccaatcaa tgggtatttg aaaaagattt
    20161 tacaatagac ggaaaagtag ccaaggaatt gaacacgacg gatgttacga ttaaaaaaga
    20221 caataattgg atttatatct tagtagcttc acttttctta ttgtttattt tattatgcat
    20281 acttttgttt gttttgagaa aaaaagaaga gaaagaaaag taaggttaag ataacaaata
    20341 gtaaattttt attattgtag gatggttggg ctctttttat aacaatataa aatataccat
    20401 tttattaata aaaatgcata ctagtaaatg atataattct aataacaggt ttgatcgcga
    20461 gaaaggccaa ataaaacgct ctccaaaatg ttaaaaaaaa gacaggacga tgtatattat
    20521 acaaacaaga atcgctgaca acagtgactc ggatccagga aatcagtttt atccccagct
    20581 agttggtgaa tgcatggaaa gtaatatacg tagaaagagc tgatgactaa tgaattatga
    20641 gtttttttta agcaaagaag atcacagaag ttattcatta cttaaatatt tagaagcaag
    20701 ctcaaattta tcagagtcaa tatcgaatgt tcaagaagag ttgtcgctat cgacatttct
    20761 cttgaaaaaa acgatcgata agctgaaaca tgatttacaa aagttcgatc tagagaaaaa
    20821 tttgcgagta accgtcagtg aattggatat atgtctggaa attaatggaa aatgttctag
    20881 caaagctctg ttatctcgtt atataacgga ttctttatct atgaaaatgg tattggcttt
    20941 ctttcaggaa aaatatatct cagtagaaaa ttttgctaaa cagcatcacg tgagttattc
    21001 tgtggcttat aaagtattac aaagactaaa aagaaatcta aaaaaatatc aaattttttt
    21061 tgaaaagaga aagttg
//
LOCUS       DACEZN010000035        20621 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEZN010000035 DACEZN010000000
VERSION     DACEZN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.035"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(305..769)
                     /locus_tag="IXH60_000970"
     CDS             complement(305..769)
                     /locus_tag="IXH60_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989927.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(769..1395)
                     /locus_tag="IXH60_000971"
     CDS             complement(769..1395)
                     /locus_tag="IXH60_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1989928.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(1402..1767)
                     /locus_tag="IXH60_000972"
     CDS             complement(1402..1767)
                     /locus_tag="IXH60_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1989929.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(1757..2095)
                     /locus_tag="IXH60_000973"
     CDS             complement(1757..2095)
                     /locus_tag="IXH60_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989930.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(2085..2411)
                     /locus_tag="IXH60_000974"
     CDS             complement(2085..2411)
                     /locus_tag="IXH60_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1989931.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(2389..2670)
                     /locus_tag="IXH60_000975"
     CDS             complement(2389..2670)
                     /locus_tag="IXH60_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1989932.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(2672..4036)
                     /locus_tag="IXH60_000976"
     CDS             complement(2672..4036)
                     /locus_tag="IXH60_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1989933.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(4049..4624)
                     /locus_tag="IXH60_000977"
     CDS             complement(4049..4624)
                     /locus_tag="IXH60_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ1989934.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(4590..5777)
                     /locus_tag="IXH60_000978"
     CDS             complement(4590..5777)
                     /locus_tag="IXH60_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1989935.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(5841..7568)
                     /locus_tag="IXH60_000979"
     CDS             complement(5841..7568)
                     /locus_tag="IXH60_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1989936.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(7565..8017)
                     /locus_tag="IXH60_000980"
     CDS             complement(7565..8017)
                     /locus_tag="IXH60_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ1989937.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(8129..8509)
                     /locus_tag="IXH60_000981"
     CDS             complement(8129..8509)
                     /locus_tag="IXH60_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1989938.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(8506..8892)
                     /locus_tag="IXH60_000982"
     CDS             complement(8506..8892)
                     /locus_tag="IXH60_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989939.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(8894..9172)
                     /locus_tag="IXH60_000983"
     CDS             complement(8894..9172)
                     /locus_tag="IXH60_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989940.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            9220..9948
                     /locus_tag="IXH60_000984"
     CDS             9220..9948
                     /locus_tag="IXH60_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAQ1989941.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(10094..10570)
                     /locus_tag="IXH60_000985"
     CDS             complement(10094..10570)
                     /locus_tag="IXH60_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAQ1989942.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(10530..10877)
                     /locus_tag="IXH60_000986"
     CDS             complement(10530..10877)
                     /locus_tag="IXH60_000986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989943.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(10864..11049)
                     /locus_tag="IXH60_000987"
     CDS             complement(10864..11049)
                     /locus_tag="IXH60_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989944.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(11050..11322)
                     /locus_tag="IXH60_000988"
     CDS             complement(11050..11322)
                     /locus_tag="IXH60_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAQ1989945.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(11319..11522)
                     /locus_tag="IXH60_000989"
     CDS             complement(11319..11522)
                     /locus_tag="IXH60_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989946.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            11647..11859
                     /locus_tag="IXH60_000990"
     CDS             11647..11859
                     /locus_tag="IXH60_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989947.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(11892..12086)
                     /locus_tag="IXH60_000991"
     CDS             complement(11892..12086)
                     /locus_tag="IXH60_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989948.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMQEPMILTRKEGS"
     gene            complement(12094..12282)
                     /locus_tag="IXH60_000992"
     CDS             complement(12094..12282)
                     /locus_tag="IXH60_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989949.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(12909..13274)
                     /locus_tag="IXH60_000993"
     CDS             complement(12909..13274)
                     /locus_tag="IXH60_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989950.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(13314..13616)
                     /locus_tag="IXH60_000994"
     CDS             complement(13314..13616)
                     /locus_tag="IXH60_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989951.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(13616..13915)
                     /locus_tag="IXH60_000995"
     CDS             complement(13616..13915)
                     /locus_tag="IXH60_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989952.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(14078..14347)
                     /locus_tag="IXH60_000996"
     CDS             complement(14078..14347)
                     /locus_tag="IXH60_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989953.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(14359..15108)
                     /locus_tag="IXH60_000997"
     CDS             complement(14359..15108)
                     /locus_tag="IXH60_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1989954.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(15111..15797)
                     /locus_tag="IXH60_000998"
     CDS             complement(15111..15797)
                     /locus_tag="IXH60_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989955.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(15803..16474)
                     /locus_tag="IXH60_000999"
     CDS             complement(15803..16474)
                     /locus_tag="IXH60_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1989956.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(16467..16808)
                     /locus_tag="IXH60_001000"
     CDS             complement(16467..16808)
                     /locus_tag="IXH60_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989957.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(16986..17684)
                     /locus_tag="IXH60_001001"
     CDS             complement(16986..17684)
                     /locus_tag="IXH60_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1989958.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            17771..18241
                     /locus_tag="IXH60_001002"
     CDS             17771..18241
                     /locus_tag="IXH60_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989959.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(18284..18463)
                     /locus_tag="IXH60_001003"
     CDS             complement(18284..18463)
                     /locus_tag="IXH60_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989960.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(18450..18788)
                     /locus_tag="IXH60_001004"
     CDS             complement(18450..18788)
                     /locus_tag="IXH60_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1989961.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(18804..19007)
                     /locus_tag="IXH60_001005"
     CDS             complement(18804..19007)
                     /locus_tag="IXH60_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1989962.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(19022..19798)
                     /locus_tag="IXH60_001006"
     CDS             complement(19022..19798)
                     /locus_tag="IXH60_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1989963.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            20097..20417
                     /locus_tag="IXH60_001007"
     CDS             20097..20417
                     /locus_tag="IXH60_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1989964.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            20435..>20621
                     /locus_tag="IXH60_001008"
     CDS             20435..>20621
                     /locus_tag="IXH60_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1989965.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHF"
BASE COUNT         5772 a         4178 c         3195 g         7476 t
ORIGIN      
        1 tcggcagcgg ctaagttcat ttctttgttg atttctttga ttgttttcga ataactgact
       61 tcgccattcg ttttaaaatt aagaacgaca tcagattctt taccagccat ttatcttagc
      121 gctcctttct accaccaagg cgatttatcc atagttacag attgaggtgg ttcaaaatcg
      181 gtgttttgtt gtaaccactg taaataagat ttgagccaca agttaggtgt agactttaaa
      241 aagaaactct cactccaatt caatagagtg agagcaacgt aaatataaaa actccaagga
      301 gttcctatct cttccgattc tttttgtttt tctttctttt tgcttgcgct ttttgaaagt
      361 cttgtggctt cttggatttt tttaggtctt ccacctgaaa tgtctggcta gcaaaaattt
      421 ccatacaggt actataagca gacaacacct ctccactcat tcccaaaaat ttgaaaatag
      481 tttctggatc ttcctctaaa ccaccagtac gcagcatggc ataaatcaag gcacgcatga
      541 tttttagatc actaggcgat aagttagcag aagaaatttg tccttctttt ttagatagca
      601 tggcgttcat atcagattca aattgcgaat aatctccacc atacacatct gctataaatt
      661 ccatcgtttg catagtaaat gaaataggga attctgcgcc ttgaataatt acaacagcag
      721 aattttttaa gtcttcaacg tgaattccat aatcagataa ccgagccatt aacccgcacc
      781 tccaacttta gccaatgttt tccactgttc ttcatcgtat acaggttgag caatgaattt
      841 attaaataat tctagagaag ctccgtctct atttgaatca aaacttgcat acatcacatt
      901 attgtatttc agtccgatgg aaaccagatt tgcagttaca tcgtcaattt ttgtttcttc
      961 ctctgcagtg gcatactctt cgtcaatcac attggatagt tgtgtattag gataccaaac
     1021 tgctttttta ccgccatttt caatatttcc aataaatcca aaggcgaagt acggaaattc
     1081 tcgtgcggta tttttgctaa acgttactcc gcttttagca actaatcctt tgatttcatc
     1141 catcacttct atcggaatac ccacgtgatc caacgcaagt tcatgttttg tttctcgcga
     1201 cacacgacga aacattttgc tggatgccca tttttctaat gctgttccat tccctttgac
     1261 accgattttt gtggcaattg gcaatcgtaa aacttcacta taagtcggtg cagttccaac
     1321 agaatctggc gttgccatca tcgcaattaa aatgtcgtcc aatccttcaa aataaaaagt
     1381 atcttgtttt cccaattaaa atcacccttc ccataaatca agtatttttc tagtcataat
     1441 ttcttcaatt ttttctttgt tttgttcata cgtaccactt gcaaaatgtt gggcacgttg
     1501 atttgttgtg ccattttcgg caaagcgcca gtaaaaggcc gtctcttcaa attgaacttt
     1561 cacttgatcg ttttcaacta ctacttttat ttgatcgcgc atatgtttct tttttaagag
     1621 cgacttagga atattaggaa gcaactgctt tagataaaag ttagctgctt cttctaaaga
     1681 ttccaacgat aattttttcg gatcaacttt tgaaaggttt cctaaataat ccgatatttc
     1741 agaaaaacca ttcttattac ttggcatttt ccacacacct cacatatgtg taatagttgg
     1801 ttacggtatc gtcgttctca tcaccttgta tacctgtaaa atctgaatat ggaatgccag
     1861 cattttgcag cgcttgttca ataacaacta aatcctgttc tgttccaagt gtaaaaaaag
     1921 agacttggta ataaggtaat tttttataga ctttaccgga tgccattttt ttgctgttac
     1981 tcacatttga gtacacaatg tatggataca tcgtccctaa tctggctttg tctctgaaca
     2041 ctgctaactt tgttgatttc agcgctgttt tcaattcatc aaagctaatc gacataagct
     2101 aaactcaact ccatttctct tgcatcggga ttcgtataaa tgcgagtaat gttatacgtt
     2161 acagaatcaa ttttgagcgc acttaatttc tctgtgatgg atttatccca tctgacttta
     2221 attcgtctga caacgtctgt cttggcttgc tgtgataaat atttttcttg agaagtcaca
     2281 ccgagttctt catagaatat tagacgctta aattcgtaaa ttgtagttgg acgatcgttt
     2341 ccgtctgttc ctgttttgat gtctagcaat tcggctttcc atctgagatt attggtctgt
     2401 ctcttcggca ttttgaatcg ctccttgcac gataaatggc gtcatggcat tcatagcttt
     2461 gtcgagttca tcctctgaaa ctctatattc ataggcaatg ccggcaacca tcaaaataag
     2521 atattcttgt tggccaccag ttgctgtttt gacataatct tttgccatat ttaaataaaa
     2581 agagagcaaa gaatcatcca tgccctcttc aaaatgaata tgtgatttga atttttcctc
     2641 taaagacaat tctttagttt gctcttccat cttaaccacc aactggtttt gtaatttcgt
     2701 agcgatatac tgccggttca aatggagaat aaaccaattg accatctagc aagttgtaaa
     2761 tttggaatcc aatttgattt ttaccagaga atttttcaac aagtttttga atttccaagg
     2821 caccaataac ttcttgaatt ttaaatgcag aaaaatcgcc aaaatataaa actggcgtgt
     2881 ctggttcacc ctttttatct gctgcatctg tccaatccac aggatagcca actaattggt
     2941 aaccaattcc accttctgct tgtgtgaatg gccgcaacaa aggaaatcca tcatctgttt
     3001 tcattttttc aatggcagtc aaagcagctc gattaataat aaagcgtccc tttttcatca
     3061 cttctgtcac tggtgtattt ttaaattcga ttaatgcatc atataatttt tgcccagcac
     3121 ctgaagcagt tagatctaaa ggttttttaa atgctacagc ctttttggct aatgcaccag
     3181 gattttcatt tccagcgtca tcaccattga acatataatt gatttcttta cgcacataag
     3241 cttttttcaa ttcttccaca acaatatctt caactggaac accagacatt tttaataatt
     3301 ttttagttac tgttgccaaa gcatcgaatt cggcaggatt aagcaaaatt tcatcaaact
     3361 gaatagctgt ttcagcaata tcagttgaac gctctttctt gtttacattc gcatctgctt
     3421 tcttcacaag aattggatat ttgacatctc ctgatgttcg caccactgtt ccgtatttac
     3481 gaagtaaatt ttcttcttga gcataagtaa taacttcaga tgcaattact tctgggacag
     3541 taactgaacc gttgccagct tcaatcccta aagctcgagc ttctgcttca gaaatatttc
     3601 cagctacaaa attagcaaat gcttttcgta gttgttgatc tttttgtttc ttggtcattt
     3661 ttgcacgtgc ctccaatccg ttcttaattg atccaagtaa tccatctcgt tgctgttgag
     3721 taatcattcc agaacgattt tctgaactat cttcactgtc tgaatcttct tcttgatcgt
     3781 tgtcttcacc agaacggctt tcgtctgaat ccgtactatt cgattgatca tctgtattgt
     3841 cgtctgtttc atcagttcca gaatctgcgc ctaattcgtc tttaattccg ttcaattcat
     3901 caataacact gtcaatttct tctttcacgg cttctaaatc tgcttcgcgt aattctcctg
     3961 attcaacttt ttcacgtaat tcagtcaatc gttgctcacg acgtgccttc atttttttca
     4021 ataattcttt atccatgtat ttttcctcct acgcttctag cgcttgatta atttttttga
     4081 ttaatttttt tctttcttcg acgttttttt ctaattcatc acgacttctt aatgcagctt
     4141 ctgtatcttc gtatgctggc aaaggcacaa tagaaacctc gtataattcc acttcatgga
     4201 ttgttcgaag cattggttca gaattgtagt cccacgtttc ttcagtcggc acaaaaccaa
     4261 aactacattg gttgatgtct cctcgttcca tagatttaac taagtccctt gctaccgttg
     4321 tgtccggtaa atcaacttcg aatttcaaac cacgttcatc ttcttcaagg cgaagggttc
     4381 cgcttttagt acgtcctagc acattggacc aatcgtgatt gaacaaacaa cgaacatccg
     4441 aattactaat tgcctttgag aatgcaccag gaacaatcac ttcgcttaag tcatcccata
     4501 aaagtgtttg actgttgaat acagcagcgt agccgctaat ggtcctcgta tgagtttctt
     4561 catcggaacg tgttgaaaga ttggtgatgt caatcgtgcg aatttccttc ttcttcattt
     4621 ccatcacctc ctttcaagtc ttgatcattc gttggtaagg aatcatctgt tgcatttttc
     4681 tggccaatcc tagataagtc atttgaaata tagatagctt gtgtttctgg agtattctgt
     4741 ttaggaaaac caagcatttc tgccacgtta tctggacttg taatcccggt acgaacgatg
     4801 ttgtagccaa tatttgtttt tgttgagtaa ggaacaaaat ccaaaatatt aattttccat
     4861 tccactctat agccagaatt aggcataaaa aaaagagcgg tgtaatgttc gctcttgttt
     4921 ttcaatattg gtttgattgc tttgttgtgc agatacatca tcgctttttc aatatctgtc
     4981 ttcatcagcg attgatacgt atcaacattg attcctagaa attttcctaa gtcttttttg
     5041 taaacaccta aataattcaa aattgccgcg tcatcaacag gactttttaa tgtctcgatg
     5101 gaatatcctt ttcccaaagg aatcatctta acagaatgat cactctcatt ttgcgttcct
     5161 tccagttgat ccaatatagc tttgacaatt tttgtttggg cgctattatt tggattgatg
     5221 tgggcgtcta gtttaagcat gaacgcaagt aaaccgcctt tagtatattt gtccgtcaaa
     5281 actttttcag cgctcagaac gccttctaga gtgtttcttg caagatcaat tattccagca
     5341 ccttttaatg aatcagttcc gatgttctta atgtgtcgaa tcatttgacc aggtattttt
     5401 tgaccattca tttcaaattc ttcttgaagt cgttcattga ttttagttgt tacaccgtac
     5461 gccaaatgaa gctgatccct atccgttaat gggaatgtct caccattgat caataaagta
     5521 tttgtttcca atttggtaaa ttcgaatcct gtcaaataat cattgggact cttcaaaata
     5581 tttagtaagt gatgatcttt gacttcgttg ccgtcgggac caataacaat tggtgtggcc
     5641 aatgccacct gattagatat gtcttgaact aattcataaa catcagaaga ttccatgata
     5701 gatgaatccg tcacatatct ttgaccataa cgtgtgtagt gtccaaaaat atcctcgata
     5761 tatccacgtt tttccataaa agaatagact gcattcgata accgatcacg taatttcaat
     5821 ttttttcacc gcctttctat ttatctatag atggaactta ggtaatcatc taactcatct
     5881 gaatcaatat ctgtcatttg attcatcgtt tccttatgac cacacaaaaa cgccacgaac
     5941 ccatcaatct ttttctttga ttgacgttta cttggcgctt tttgtccatt gatgttagta
     6001 attgctacaa cgttcaaagt gcaataaagg aataatggat tatcaaattg aattcgtttc
     6061 tcataaaaca accgttcgac atcgtcaaaa ggagcattca atactttagg atattgagca
     6121 acttcaacgc attccaatcc taagttttct aatttctcaa caagtttgtc actcatcgct
     6181 ggatcataat tcacttgctg aatatcatat aaattcatgc agtcctctat gtactgcaaa
     6241 atctgatctt gatcaatcat ttttccgtcg caaaattcaa cgaaaccttg ttcagccaaa
     6301 tcactgtaag gaacattatc ttctttttct cgaaactcta aatcttcatt cggaataaaa
     6361 taaagttgct ttactttaag aaccgcttta ccatcttcat cccatgttgg gaagtttaaa
     6421 gatacacagg tcaaatctcg tgtacgtgat aaatctaaac cgatataaca tggttcgcca
     6481 cttaaatcac caagttcttg tgtggtaact aaacatggct ctacttgatc ctgttcaaag
     6541 aaattatccg cgccgttcac aaatacatcc aagtgtttcg ttagaaattc agctttggaa
     6601 tgagctgacc gttgcgctgt tttaaatgcc gattctaaag cagataaatc gactgatatt
     6661 ccccagttag gattgcacat ttcccagact tttctatccg tccaatcata atttttattt
     6721 ggctcataga ttagaacaaa gtttgaatca ttatcatcac gtttcaacac ttcttttgct
     6781 tcgcgatata cacgcattcc aacagacgac gaacctttcc cagcagttga gatattaaac
     6841 atcaatggct gtggtaaaga tatctgagca gacttaaaat tgtcatactg ttccattttc
     6901 tcctgtttat gcaattcatc gttcaataca aaatatggat tggatccctc gatattatca
     6961 atattcttgg tttgaacaat aaatttattt gagtaagcca tatcttcatg aatatagtca
     7021 taagtaatac tagaaacagt tccctttggc cccctaaata ttttagttcc ttcaagtagg
     7081 attggattgt ttaagattgt aactgcaaaa ggtttggcag catattgcgc ttgagcaaaa
     7141 tcagatgcgc aagcatagca atcaactgat aacgcccctt ctccgtacat tgcatatcct
     7201 aatgaaccga cagcaatcaa tgttttacca ttcttttttg gaatctggac ataagcctca
     7261 cgagtaacgc gtacaatttg gcctttttca ttttctttaa cccaaccata aatccaagaa
     7321 taaataaatt tttcccaggg ttctaaaaga aacggttttc ccaccatttc tccttttgtg
     7381 tgaacaataa atgattctac ccaatccatc atttcattcg cgcgatctac atcaaaccaa
     7441 atatcttttc tttttttcca ctgataccag cgatctattg ctaaacgaac ggtcttagga
     7501 tacttcttag gtttctttct tacttctttt gcaaataaat cagcataatt tacaccaggt
     7561 tcaatcattt ttcagcacct gccttcttac gccatttatt tctgtgcgct gctagttcat
     7621 ctactggctt tacttctggg cgtgtaattt cttcatcttt tcttgcggtc gatcctccag
     7681 caattagtct accagttttt gctttgtttg ttaatcctaa taaatctaga gctttcatct
     7741 tcttatctgc ccaagtttct acttgttgtg ccaatggatg ttttgattga ttagtagctc
     7801 ctgatttatt cgtgaatttt tgcgtctccg gaaatccttt ttccttccac aataaatatt
     7861 tgtgttggta aatttcaaaa atatccaagt atgattcgat taatggatca agagtgatgg
     7921 tgtacaaatc agacaaattc attatttcta agatacgagc tttttcagca cttacttttt
     7981 catcaataat cgctttacgt tgcgctttag tcgtcatttt tgtatacacc cccctttgtt
     8041 ttttgaaaaa tttgacctaa cgatgcgcgt gactccccgc taccctatct ccccacgcga
     8101 aaaaattttg aaaatgaata ggggggcttc aattgaaata cgaaggaaaa acttttttgt
     8161 cttccgtttc gttttcaaca attggatgac attttgaaca taatagcatg agattgtttg
     8221 gatcaagctt aagcagttcg ttgtctttga ttggtacaat gtgatggacg tgtgctctct
     8281 ttccaaatat gaactgacca caccgctgac aatgaccacc ttctctttca taaataaatt
     8341 gacgcatatc tttccatgct tgcgttcgat agaatggttt gttctcatga tgataaacag
     8401 actttgcttg ctgcttcttc ttgcgtgatc tgcttgatct cttgtgttca gtacagtaga
     8461 taccctttgc tattttgttc gtgcatccgt caaactgaca gtatttcatt ctacttcacg
     8521 aataagattt atgatatcgc cttttgcacg gatagcacca ggaatatcaa taccatgttt
     8581 cttagcatat gcacgcaatt cttttgcagt catattgtcc agttcatccg tatcttcagt
     8641 ggactgatca ttagtagctt cgtctccatc aaaattagca gcagtgtttc catcgctatc
     8701 aagaatttca ctgctatgag ctatgagttc gccattgaca gcaacaaatg atttgccatc
     8761 actcaaaatt cctttttctt cttttacttc aaaatttggt tcttgatctt tcggaacaac
     8821 tactgtcctt ttcttttctg aatcccaata ctctgttcca ctaatggatg ttctaatttt
     8881 gatcattgcc attttaattc tctcctttgt aatttgtacg gattactttt gcgcccattc
     8941 gctcatacca ttcaatcgtt tcctgcagat tgggtagagt gtgtgacaat aaagaaataa
     9001 ctaacgtatt cattccagtt ctgtaatcat cattctccac ttgcacatgt gcatgattac
     9061 cattccaatt ttctctgtac ggactattct ctgcttcaat tgctttctta taaactttag
     9121 ccaaacttga ttctacttca atactaagta ccgcttcatg aaaatcattc atttgtttac
     9181 tctcctatca gatattttgt ggtatgattt ataaaaaaga tgcgaggtgt tttaatgctt
     9241 caagaagtta atattaagta tcgtgatcgt caaaatacag agacaattta tttgccaaat
     9301 agttgtccgc attgcggaaa tataatgact ccgtatgtgt attttggggt ttcatcatat
     9361 agtgcgttcg ataatgatag aatctttggc gtattagtac agtgtataaa tgatgagtgc
     9421 aacaaatttt atagtttaca atttataaat acgtttaatg aagatgaagg atattctgaa
     9481 aatattttaa ttaaagatta ttaccgtccg ccgattaaaa tagatttacc agaaaatatt
     9541 gaaaaagttt ctcctacatt tgttgatatt tatactcaag ccacacaagc taaatctgag
     9601 caattggaac aaattgctgg agttggttat agaaaagctc tagaatttct aattaaagat
     9661 tatgcaatat ctaaaaattc agaggatgaa gataaaataa aaaaaatgtt attaggtaaa
     9721 acaattgaaa attatttgaa tgattttcct aaactccaaa gattagccaa agctgctact
     9781 tggattggta atgatgaaac gcactatgtt cgtagacata ctaataaaga tgttcatgat
     9841 atggatgcat ttattaaatc agcagcgcaa tttatcgcgg ctgattatga tgctgattta
     9901 gctgatcaat ttataaatgg aaataacttt atgccaacta ctaattaaaa gaataaaaga
     9961 gccacagata ccaattaata aaacggttgg gatatcttgg ctctttcata tttttttgac
    10021 actaatagaa tatcatgctg ctaacaatgt ttacatagct acaatgaccg aacatttagt
    10081 gaacagtgta attttagaat aaagagagtt gttctttgta cttctcgcta ttgtaaaaat
    10141 caatttcgtt ttcttttttc aatcggtcag cttgtctgtg ttcatactca tctaaaaagt
    10201 tcaatgtctt tctaatttct gcatgtcgct gtctgacata cgatgagcta tacccaacag
    10261 attcagcaat atcttctaag ctcatttgat caacatattt catcttaaca atttgattat
    10321 cgataccaga aaagctatct atcagcaata acatttcttc tttttgctcg atcaacaatt
    10381 ccaattcatt ttctatcttt tggatattct cttctaaaga agaagttctc gaattctttt
    10441 cgatgcggac gtttgctaaa tcaccacaga cccatcgatt caattcaagc ttacttttat
    10501 taagattcca ctttaagtaa agaatctgct catctagttc ttggtaatct tttaaccaat
    10561 gaaatctcac aaacgccacc ccttatggta gaatagtatt gcgctgctat ctcgaaaggg
    10621 gtagcttttt tattttttct tagccgttcc attctggatt gccttttgtt ccaaacgtcg
    10681 ttttttcttc ttaatctttg attttgtttt tcccatacaa ttctcctttc cgaaaacaca
    10741 ttttgttcga tttttaatac taaataaaag ttggccagaa tttttcgttc gtctttactc
    10801 atcttttttt gaataagatt actagcataa aaatagttag taccacgaaa atgaacaggt
    10861 aaatcaatta cgttcacctt cttcatgcca gttatcagcc actagaatct ttgcgttagt
    10921 gccgccaccg ttttcttttt tgtatttttc aaatgcttta taagcagctg tttttgattt
    10981 atagattttt ccggaaccga ctgggaacat ccatgtgccc tctgtgatag ctaagatgta
    11041 tactttcatt taattcaccc ctttaatccc caacactaca tatccggact tctgcatgta
    11101 atcagtaata taagtgatct ctgctttgta tgagtcacca gtgtaactaa atgttggtct
    11161 cgttagtcca ggtaattcat gtttaatgta ttttttatat tcacgtagta ttaactgatc
    11221 accaactttg aagtcacgat cattcttgcg gatctcgaat tgtttgcgtc cgctcgtcac
    11281 tgcttcaaaa tattctggta gtatttttag ttcgtggatc attctgtttc ctccttcaaa
    11341 attcgctcat catcaaaaag tgaatctcca caaaatcctt ctcccaaata tttatcgttc
    11401 atgtataagt caaccaaata tttgtgatct gcttctcgat attcattaat attaacgatc
    11461 tttattttgt aaacattatc ttcgtcgctc actatcttga attgttctct gactttcacc
    11521 accgttcctc ctctttttga aaaaatttta gtgtttttgt aagaccccct aaatcagctt
    11581 ttttatttga tataatactt gtatcgatta atcaaatcga aattaaattt aaaaaggtga
    11641 tttatcatga atatcaacaa tttagtaaaa ctcgttgaac aacgttttaa agaatcagga
    11701 ttagaacaat taaaaaaaga taattctcca gaattatttt ctaattttac cgaagacgac
    11761 attcctactc ttaaacaatt aaaatattat tctgattatt ggacgcaaac tcgtgagaat
    11821 gaattaaatt tagaaaaact cattattaaa aagaaatagc aactccagcc cacacggctg
    11881 gttttttagt ttcaacttcc ctccttacgg gtcaaaatca tcggttcctg cataatctga
    11941 tcatccttta gcaacaaatc aggatatttt ttccttagct tcttattgtt cgtgtaagtt
    12001 ttcaatagcc attggttggc ttcgcttttg ctttctccag aaaaataaat tttccctgtt
    12061 tggatgcctg caagttgaat catcattttc acctcaaatc aaattttttt ctgctagatg
    12121 gagcaaaagc aatccgacat caagaatctt ttgacccatt atttcagcta ctttgctcgg
    12181 cttaactcct tgagaaaaga gcaagcgtgc ttgctctatc tcttcgtgag tccaaataaa
    12241 tttggcttcc tcaaggataa ttactggatc agttgctctc attgttcctc tggaaaaatg
    12301 atgtcattct tatgcttgga ccaggtatct gcatatggaa caaagtattg tctagctaat
    12361 tcgatttgat caattaaagc agtttctgat aattcatggt ctgcagcaat ttcattcata
    12421 ctatcgcctt catctaaaca gataagaccg ttgacgttat cgatttttac atttgtgact
    12481 gcttgacttt ctccacgctc ataatattct acagttgata ctgatacatc agcttcggag
    12541 tttaattgca gaatctgtta ctgccgggat aatttaatat tcttttcata attcaccttt
    12601 tttcctactg acatcgaata tttttcatgt tatgattagc cgtgaaagga gagctatatt
    12661 atgtgtttac acatgatgtc cttagctcat tcggttcttt ggaagctttt ttggaaataa
    12721 ctctcaggtt cttgacgcaa atctcattta ttctttgaaa caaaaaactt ccattttccg
    12781 agagctttgg atcacttcaa aagagatttg catttatatt tctgtgacct tgagtcgcgt
    12841 aaagaacctt acagcaatgg cggcaattcc actttcagga aaccgccttt tttcattgat
    12901 attggtggtt agcggactac ccattttctt tcggcagtgc caaaccacaa atgatctgtg
    12961 ttgatgttta ggtagttgta taccagataa gctaaatcca aagtgcctgc atcttcaaga
    13021 cgacaatatt ccaaaaattc tcccatagct ggacataaaa atcctggagc tatcatatcc
    13081 caacagttaa tagtgcaaag aaattcgttt agcttctgaa cgttttcttt ggtttcacga
    13141 attacaacat actccaattc ttctccgctg gcagagcatt ccaaaacttc aaattgatta
    13201 agaaatggta gaacatatgc ttcaaacatc aacattccat ctttctgttt ttgcacttgt
    13261 tttgcatcaa tcatgtctat cctcctattc agttatttct tccattaatg gaattatttc
    13321 aggtcactgg acttaacgaa aacaccattg accatctttc cagtccgacc tttgatttcg
    13381 tcataagcaa aatccaagca ctcgtataaa tccatatcat tttgcattgc caagatgatc
    13441 aaggttacta ctacatcgcc aattccgtct cttaaaccat gttcatcttt tctagctaga
    13501 gaagcggcaa cttccccgat ttcttcgacc gtttttaaca tttgcttgct gaaatccgct
    13561 tgatccaatc ccttatcttt tgcccactgc tctactttcg cgattagttc gtccattatt
    13621 tctcctccac atacctaaac tgtcttcctt ttgaatcaat ccataagctc ctagctctat
    13681 cccaaataat atttttgctc agaccagtaa tttcagataa ctgtttagcg gtacccgtta
    13741 ctagaattcg atcaccatgc cagattgcaa tctttctcgg cgttctccgt ttgggctttt
    13801 cagtccacat tgatttaccg agcttttgga cttctgcaac tattttttta tcttcctgcc
    13861 aatcttctga ataagtcaat tcgataattc gctgcattgc cgctttctta tccatcccga
    13921 cattctcctt tcagtaattt gagtacttga tcaagtgcgc tctcacgtcc gccatgaaat
    13981 gtgttgagcc acttgtcttc gtacgacaca ctttgtctta aagcttcttg atgcattaat
    14041 tcgatctgtg ctgtaaatgt ctttaggtcc atcttgttta cacctgctca agttcattaa
    14101 gatgtttttg caagccttta acacaatcaa tgaatagtaa tttggtatat gctaaatttc
    14161 ttaattgcgt tccatcgata tagagtgcga aatagtatct gagtttactc caactcgaac
    14221 gatcattctt aattccttca attccagctt cttcgagttg atcatatacg tctctcagga
    14281 cgtctaattc ctcgcccttt ttataattcg ctatttcatt aattagttct agataatcga
    14341 tcttcatttt tccacctctt agaatggtgc ttttgattgt ctattagctc gttctagcgc
    14401 ttttttcttg agataggctt cttggtcgat tgcccattca ggaagcttct ctcgtcttcc
    14461 tgtgcgcttg tatccactgc ttgcgttctt aggttcactt ttttctttcc ttgcccaact
    14521 tcgaatagtt gccaaatagt ttttataagt cttaccagat gattcacaat actctgacag
    14581 tcgttcgatt cgctcttggt aatcattagg gaattctgtt ttgagtttct ccatctgctc
    14641 atctgacaaa agaacatttt tatactctcc gtatttatga cggacgggct tagccttcga
    14701 ttttttcgaa ggcggtaact ctcttatata ttcttttgta ttattaaatg tattattaat
    14761 agatgtatta ttatctttga ctttttcgtc aataggggta ttgcgttttt cgtcaatagg
    14821 gtattgatta attcgtaggt acctattgat tatttgattg gtaccctctt tgtaaatgat
    14881 ttcccgattc aagtatccaa acttaatcaa atcacttacc catcgcgata tggtctcttt
    14941 attcacacca tataaatctg caaagtactc attgcctgcc caacaaaagc ctctttcatt
    15001 acacaaggcc gttatctctc cgtataacaa cttagtattt ggtttaagtc ttttgtcgta
    15061 ccttacgttg gctggtataa tcgcataata acttcgatgt tctgtcattt ttaccctcca
    15121 atatttaact ttttgattgt tttctggttt aatttgatcc ctttgatttg atatttattt
    15181 ttgaaattaa tcacacctat tttgtgcttc tctgtgtgat ggattctgca gagtgctgca
    15241 aatgtgtact ctgcatgatc aacttctttg cgctttcgtc ttcctagcgc tttgtcaaag
    15301 tgatcgatgt cagctcctgt tttgccacag atgcagcaga ctctttttgt gatgcatttg
    15361 tagaagtaat attcttgatt cgcaggtaaa atctcatagc cttctttgaa aggaatatga
    15421 tgttcaaaga tgaaatctaa gatgatattt gctaagacat tggcatcact cacagttgta
    15481 ttcgattcgt ctttcaggct tattttgcgc cctgtgacac cttcaaaacg aaagtagaag
    15541 aattccttcc agaagtccgt tggcatgcct gtatcgataa aaatatcgcc tatcagcgca
    15601 tagatgaagt ttcgttgctg tacggtgaaa cgtctaggat caataaaacg aatttcaatg
    15661 actcgatcac catcgtagcc gtcatacatc gtctttagtc gatcaatgtt cacttcctca
    15721 ttgatggttg cgcttatgtc ttttcctttg aactttttca gaaccgctga atatgaatcg
    15781 attaatggtt taaacactca tatcacttct tatctaattc ttttctctta gctgctattg
    15841 ctcgctccat caaggcacat tgctcatagc ttaactgttc aatagtttca acgttatcag
    15901 ctaagagccc taatttatct gtctgctcat taacatattc gattaaggtt ttggtcatat
    15961 ctttacccat ctgctcattg aaagcttcta gaatcgtctc tagcatgttt aatttctttg
    16021 tatcgattct aggtggtgtt ggaatatctt ccccttgaaa tacatataat cccagtccgt
    16081 gtagagccaa tgctttcaca aagcatcgct tcaatgagtt attgatttgc attgcatttg
    16141 gtttaacaac tggttggttt cgataatcta aaacaggaaa taattcggtt tccgtgtgtc
    16201 ctttaaccgt tactgagaca gatacataag tcccagtttc atccataaga aaaggtttat
    16261 attcctcaac aagaaagtct tgatgagttc cagaaacaac cctgtagtgt ttgtactcat
    16321 taatagttac cgttgcctgt ggatcattct ttttcataat ctcccacgcg tgagcccaag
    16381 ataaataatc aaaatttcct tttttcttga gaattttatt taacttacga ctaaaaagtt
    16441 tttcaaagtt cgttgtccct ttgatttcac tcatcaaatt ctgcctccat ttcagcaatg
    16501 tatttcttac ctggtccgta ataagagata tcaatcaagt tatctctgtc gtactcttct
    16561 agcgcatcaa tcaagccatc ttcgatgacg taaatatatt caggtttatt cgaatgcttc
    16621 gatagatgga taagataaac atgatcccaa atagttacat ggttgcctaa atcatcttga
    16681 tcccaagcta gttcttcatt cgtcaaaaga tttcgtctga tttttcgacc acttgtttcc
    16741 tcaattttcg gcttgcccca atcaggatca atcaaatatt gatctagagt ggaaagttct
    16801 ttttccatat gctaaaatct ccttatgatg tgttttcttt gtgactcttt gcttgccggc
    16861 ggagtcactt ttttatttgt tgccatgctt tttgcttgtc aatatgttgt tggcttagga
    16921 tgattggttt atagtatttc caccagcaat tagcaattgc cgtccctatt cttagcgctt
    16981 cagctctatt cattgtcatc accaaaaagt ctctgttgtc tgttcagttg atcaatttcc
    17041 atacggatcg cagtttctgg caaccacatt tcaataaatg aaacagcatc atcgaatctc
    17101 ttacgaggta actcgccata tcttgggatt gaaaaggtac gtttaaattc agaccaaaat
    17161 tttgagaata cttttttgct gatttcttca taagctcggc tttcttttcc ccctagaact
    17221 cccataactt tcatatttcc tttttgctta atttcaaact cttgttgtcc gctaattcgc
    17281 atagtatctt taagcatgga aacatctttt ttaacatctt tcatttcttc taattgatag
    17341 atcatcatat cttcaattgt ttgaggaaca gtattcttgc gaataacatc ttccatttcg
    17401 ttgaatgctt caatgtattt ttgtttgaag tagatagctt tctttcctgt aaaccccata
    17461 gccagcaaga aaaatccatc tctactaatg aagaaaactc gtcgatttct gccatatgaa
    17521 tctggttcat taccttctac aaacatctgt ccaaaattgg acacatcttt ttttagtgca
    17581 tcaatatctc ttaaaacatg ttgatgtttt ttctcgaagc tttctgccac ttgtaagctc
    17641 gtagtcacag cttctttatt tttcaaaatt actaattctt gcattatttc ttctctcctt
    17701 tttggtataa tttagttaaa aactggtggt gtttattttg aatttttttc acttaacaat
    17761 ttttcaatgg atgtcaataa taagttttat attgagtacg tttttaacag ggataaaaat
    17821 aaaaggaatg cgccctcaac tagaagttga acttaatgct tcttacttcg ctgcagatat
    17881 gatttataca aaagtaatca tttctaacta ttcaacagaa ccagctatgt tagttaattt
    17941 agaactttca tccgctaact taaaccgcag atggtcagct actccattta agaaattaat
    18001 tgctaaagga ggatcagtcg atgataatag aatatactct gaagctgtac cgctaaatat
    18061 tcctcctaaa agtgctatat cttgttacct tgcatttgaa gtagggaaaa ttaattttaa
    18121 taaactcctt aatcaccaaa caaaaatgat atttactcta aatagaaccc aaatccacaa
    18181 aattgttgat atcaaaaata caaacttccc aatagagaaa ttagtgaaag aattaaactg
    18241 agtaatagta atatacattc tagtttctct tttctattca tttttagtca gccccctcgg
    18301 ttggcttttt cgctctgtac tcagcttcag cttcatcaag ccccataaaa atccaaacca
    18361 tgtaaacaat cgttcctatc aacgcttgcc tgcttcccca aagtcctaaa gcgtagatga
    18421 ttagcggtgc gctgaacact agcgctctat taaattttcc catccgcttg cctccttaag
    18481 atttccgaaa aatttgtttc taaaaattca agtgttttac ttcttaaaaa tagatatgtg
    18541 tctcttcctt caactggata atagacaaat ccatttttgt ttttctcgat atcgataata
    18601 tttctatatc ttgggttctt taaaactcta gaagtaaacc aatcatattt tctgttaatc
    18661 cgttctagca cttctggcaa cgtcatccat ctaccagtat catcagcttt ttttaactcc
    18721 tcataatcca cttgggagat aattacatag ccttctggaa ttgggatttt tgcttctaga
    18781 tattgcatta gctgttcctc cctttacaat tcgtacatag tgataatcga atctataatt
    18841 ctgtttgctt ctgcagaagt ctttttaccg tttaaaatta aagataagta gcttttgcta
    18901 attccaaatc tttcagcaag catggtgtaa gttaagaact ttgaactttc gacatattct
    18961 ttgatttttt ctctatctcg ttgagtgatt tctgcaatgt cagtcatact aaaactcctt
    19021 tctaaccaat ttcctctaaa tccatttgag ggtaatatcc ttcttttttt agtaattcgt
    19081 agataaatag acgccctttc tgtgtccatt tggtattcat tacaatttta gtgccaccat
    19141 cggctttcgg gatctcagtt gtatgagatt ttgtgtatcc ttggttcatg tgttttttgc
    19201 acaataacca ttggttaccg acttttttct gaatacctag tttatgaagt aatttattca
    19261 tctgttgtgg agacatccca taatctgctg caatctgact aattgttact gaatctgtag
    19321 aagataatat gctatctaaa taggagattt taggttcgta ctctgcaatc ttttgttctg
    19381 cgattaatct tccagttctt tcttctttta gttgagttgc taattgaatg attgtatctg
    19441 gattaagcaa agcttcttct actttttctg gagttagata acctccatgt tttctaattg
    19501 ctggcaacac ttcacttgtt acccaacgtt taaatttttt ggcagaggga agttttgatt
    19561 ttaagattaa actgtacaaa ccagattcgt tgatgattgt catctctctt gattgacctg
    19621 aggtcgtgat tcgcgacccc atcttatctt ccaaatctac atgccgcgac aaagcatctt
    19681 tagtgtttga ataacccaaa acactcgcaa cgtctttgcc tacaaaatat ggttcatcat
    19741 ttactaaaat tgtccgaact tcgttttgtt cgaaattaaa aatttgcggt gtgttcatat
    19801 ttctcattcc tttcttttat attcgtaaac aaatttaaca acttttttct aaattcaatt
    19861 gacagtatct gagtaatact ctatactaag agcataatta aataagacat aaaaacattg
    19921 attttaaagc tttcttggcg gtctgcattt acatatcaat agtgtttttt gttgtctttt
    19981 tagttgttaa atctgtttac aagatatagt atagctcatt actcagaatt agtcaaatta
    20041 tttctgcgta tttttctaaa ctttttttgt aaacatttag aaaggttgtt ttatcgatga
    20101 atacctatga aataataaaa gacttggcga agaggaaaaa gatatctata cggcagttag
    20161 aaattaattt tggatattct aacggttatc tagcaaagtg gaaaaataat actcctaatt
    20221 cagatgaatt aagtcggatt gctgactatt tcgatgtctc tgtagactac cttctaggta
    20281 gagaagaaag agaaacccct aaacatgtgg atttatcaga agacgatact gttttttctt
    20341 ttgatggaaa agaaatatct aaggagacaa tgcgtaaagc gattgcaatt gctaaagctt
    20401 tagaggaaaa tgaatagttg gagtgatggt ttgtatgtat ttgaaattga aagaaatgtt
    20461 gagcgaatac aatttgaaag tagtttatat ggagatgaaa gagcctggtt tctactatcc
    20521 aaaacccaga atcatttttt tgaatgaaaa tctatacggt gaaactgcag aagcctttca
    20581 tttatctcat gaacttgcac atttcagcgc ctctcatttt g
//
LOCUS       DACEZN010000036        20521 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEZN010000036 DACEZN010000000
VERSION     DACEZN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.036"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(434..1747)
                     /locus_tag="IXH60_001009"
     CDS             complement(434..1747)
                     /locus_tag="IXH60_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1989966.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(1992..2216)
                     /locus_tag="IXH60_001010"
     CDS             complement(1992..2216)
                     /locus_tag="IXH60_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989967.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(2236..2604)
                     /locus_tag="IXH60_001011"
     CDS             complement(2236..2604)
                     /locus_tag="IXH60_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1989968.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(2800..3510)
                     /locus_tag="IXH60_001012"
     CDS             complement(2800..3510)
                     /locus_tag="IXH60_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1989969.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(3528..4024)
                     /locus_tag="IXH60_001013"
                     /pseudo
     CDS             complement(3528..4024)
                     /locus_tag="IXH60_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(4061..4513)
                     /locus_tag="IXH60_001014"
     CDS             complement(4061..4513)
                     /locus_tag="IXH60_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1989970.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(4648..5253)
                     /locus_tag="IXH60_001015"
     CDS             complement(4648..5253)
                     /locus_tag="IXH60_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1989971.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(5703..6323)
                     /locus_tag="IXH60_001016"
     CDS             complement(5703..6323)
                     /locus_tag="IXH60_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1989972.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(6325..7194)
                     /locus_tag="IXH60_001017"
     CDS             complement(6325..7194)
                     /locus_tag="IXH60_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1989973.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(7217..7624)
                     /locus_tag="IXH60_001018"
     CDS             complement(7217..7624)
                     /locus_tag="IXH60_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1989974.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(7643..8932)
                     /locus_tag="IXH60_001019"
     CDS             complement(7643..8932)
                     /locus_tag="IXH60_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1989975.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(8959..9261)
                     /locus_tag="IXH60_001020"
     CDS             complement(8959..9261)
                     /locus_tag="IXH60_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1989976.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(9286..11379)
                     /locus_tag="IXH60_001021"
     CDS             complement(9286..11379)
                     /locus_tag="IXH60_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1989977.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(11868..12596)
                     /locus_tag="IXH60_001022"
     CDS             complement(11868..12596)
                     /locus_tag="IXH60_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1989978.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(12742..15363)
                     /locus_tag="IXH60_001023"
                     /pseudo
     CDS             complement(12742..15363)
                     /locus_tag="IXH60_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(15477..16808)
                     /locus_tag="IXH60_001024"
     CDS             complement(15477..16808)
                     /locus_tag="IXH60_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1989979.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQLIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(16774..18252)
                     /locus_tag="IXH60_001025"
     CDS             complement(16774..18252)
                     /locus_tag="IXH60_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1989980.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(18557..20392)
                     /gene="tdc"
                     /locus_tag="IXH60_001026"
     CDS             complement(18557..20392)
                     /gene="tdc"
                     /locus_tag="IXH60_001026"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1989981.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT         5928 a         4407 c         3442 g         6744 t
ORIGIN      
        1 atggatgttt ctattttttg cttaatcatt tattttagtc tctctcacta aaaattgtaa
       61 cgctttcaaa tagctattgc accttgttat cggtaacaga ataaaacaaa aaaacagctg
      121 aggaaattcc ctcaactgtt ttttgaaagg attgatttat tcagggaaga atgctgattt
      181 cacaacttct ttataaacgg tgttcactca gctgtccctc ggtattagct caaaaaacgg
      241 caaaatataa gaaactattt ttttcgtttt tttctctaat actcaggcca gaacactgat
      301 ttcacaacct ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaaa
      361 ggcaaaatat gaggaactat tttttcgttt ttttctctaa tactcaggcc agaacactga
      421 tttcacaacc tctttatttc acattcaata tccacatgaa tcccatgaat acaaagaaca
      481 gaccatacat gatgggagat acttcttttc ctcttttagc tgctaacatg gtaattggat
      541 agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca
      601 agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt
      661 gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg
      721 atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga
      781 tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag
      841 aagtacctaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta
      901 cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc
      961 ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag
     1021 aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg
     1081 cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag
     1141 ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga
     1201 ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg
     1261 tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagca ataaatagac
     1321 cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc
     1381 gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg
     1441 ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc
     1501 caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa atacagccga
     1561 tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta
     1621 atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca
     1681 gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt
     1741 ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat
     1801 ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat
     1861 tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca
     1921 tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct
     1981 agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg
     2041 aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt
     2101 tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca
     2161 ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt
     2221 tctcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc
     2281 agggtccgaa aaattgaaag tcaagacgcc attgttgtct gtgatttctc ctggttcttc
     2341 catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc
     2401 taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc
     2461 ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat
     2521 ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag
     2581 catcacacgt gttttttgta tcatcgtttg acccttcttt ctatttgatt ttctttcctg
     2641 tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca
     2701 agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa
     2761 aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc
     2821 atctttggtt agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt
     2881 caacgcttcg attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac
     2941 cagcaccgct gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag
     3001 acagcgacag atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc
     3061 atcggaatct gcacctaaag gcaggaatga ttcgtcacct tgggcttttc ctaaatgcag
     3121 atttcctgcg atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat
     3181 actattatag atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg
     3241 tttcagtaaa gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc
     3301 ctgacgccac tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct
     3361 tgcttctttt gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac
     3421 gtggttatcg ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca
     3481 gaaattggta tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc
     3541 ttgatgaata gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat
     3601 ttgataaatc cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg
     3661 agccaaatgg ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat
     3721 gtaccgatac cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa
     3781 tgatcagcca tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc
     3841 tcttgatcga tactcactag gaaaaaatct gtttctttat cttttagctc ttctgtaagt
     3901 ttttctagtg cataagcttg tgtcaagtaa tcagctaaat gctccggtaa ttatcttttc
     3961 caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg
     4021 tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc
     4081 cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt
     4141 ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt
     4201 tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga
     4261 tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga
     4321 ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga
     4381 ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt
     4441 ctttgaattc gatattgcta atagagtcta gcgctcgggc aatcattccg atgtctcgaa
     4501 ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttta cctctttttg
     4561 ttgcaaatgc aactattttt aataaaaaaa tcaagttctt ttttaataac tagtaaagta
     4621 tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa
     4681 aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact
     4741 aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat
     4801 cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt
     4861 cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac
     4921 ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga
     4981 ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc
     5041 acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc
     5101 ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc
     5161 aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag
     5221 tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt
     5281 atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat
     5341 ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg
     5401 gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca tcaaacgaaa
     5461 aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca
     5521 caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca
     5581 tcaaacatga gaagctgttt ctttgaaatt gctccttgtg actcggagct gaacgacagg
     5641 ctcacgacct ctcggtaaac ggtgttcaaa agccaatttt tcgatattaa gccatttctt
     5701 tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact
     5761 tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt
     5821 cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga
     5881 tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat
     5941 tcacgatcac gtttgtaccg acgttgccat aacacttcaa agtctgctgt taggcgaatc
     6001 gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca
     6061 ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct
     6121 ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta
     6181 aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct
     6241 ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc
     6301 agaagaatga aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt
     6361 cataaacagt ccaatcttat gcttgacaat ttcttttact gctttatttg ctggaatcac
     6421 attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt
     6481 ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc
     6541 aggataatct tcttctttca cacctgttcc tccatggata actaggggga catcaactac
     6601 tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg
     6661 attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc
     6721 gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc
     6781 gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac
     6841 tgcttttgtc ttcaagatat tttctttgaa aggtagatgg gacccgtcat acatgacaga
     6901 agtatatccg ctatcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg
     6961 taagactgca tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg
     7021 catgccgata tatttagcgg tatcgacact tgtttgaata atgattggcg aacccatttc
     7081 ttttgctgcg cctagcatgg caggaagcat ttctaggtta tgcgtattaa aagctccaac
     7141 tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta
     7201 ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca
     7261 tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat
     7321 ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt
     7381 tattttccaa gctcttttct aaatattgga tggcttgatc gttctcatgc aattcagaaa
     7441 atttaagtcc aacttgttca taaagttccg ctaatttatg cctatcttgg gtagtggaaa
     7501 tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg
     7561 ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa
     7621 acatcatttt ccctactttc aattaaaacg ctttcaaaac tgggcttaag atccaggcat
     7681 ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgatg aatcccatcg
     7741 aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac
     7801 cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc
     7861 cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag
     7921 taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acggcatttg
     7981 ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca
     8041 tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca
     8101 atatatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt
     8161 gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata
     8221 gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg
     8281 gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa
     8341 caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc
     8401 cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc
     8461 cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga
     8521 ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt
     8581 ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat
     8641 ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga
     8701 tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga
     8761 tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga
     8821 tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg
     8881 tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgctc atgtttttac
     8941 cctcttttct ttttgtattt agataactcc cttatcttgc agaactgggg tgattttttc
     9001 ttcaatttca ttttttgaaa caatattttt caaatagatc atcgccgttt taggatcaac
     9061 tgcgaacttt tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat ctggtttcat
     9121 ggaaactgcg gaagaaatat cacaatggtc cagtttggct tctactccta atttgttcaa
     9181 aacatcctca gcagacatct gtgctgcaaa actacttcct aaacctgcgc cgcatacgaa
     9241 taaaatgttc aatttgttca tctttgttct cccccgtatc atttattatt tctcaactac
     9301 tgaaaaaagc agttgtttga aatcttcgat atttgtgcat tcaatcaatc gatccaactt
     9361 ctcttcgttg ttgatcaatt caattagttc tttcatgatg ttcagatggg agtaggagtc
     9421 tactgctgcc aaacaaaaaa taatctttac tggatccatt tcaggattac cgaaatcgat
     9481 tggttgctca atcgtagcta cgctgactcc taatttattg accccatctt ctggtcgggc
     9541 atgggctagt gccatgtgtt tgccaatcac gatatacggt ccgtaatcat tcactgcacg
     9601 aatcatcgct tcgacatagc gctcttcgac aatgttttct ttaaccaatg gtctagcagc
     9661 tagttcaatc gattcctccc aagtagtggc tttttctttg atcatgatat tgctgtccgt
     9721 caaaatatct tttaacatag gctggatctc ccttttgttg atttctaaat tgtttgtttc
     9781 gaataatttt tctaacttgc catagatgat tcgatctact cggccaccac tttctttcat
     9841 gacatctatt atttgagtga ataactccgt cgattcatga ctgttaggct ctaaccgctg
     9901 tagttcttca tttcttttca aaaagtcttc tataaccaag atattcgtct cagttaaaat
     9961 cggatcgaca actaaaagcg gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag
    10021 attgacatcc aatttatcta ctaattcgat ttcttttgaa cttaaaactg cagtaatctc
    10081 gatctgagga aacttttctt tcagattctc tgctaacaga tttccagtag ccatcccatg
    10141 gttacagaca acgactgatt tataaacata attcaagtct cgcttgatta cactggcgat
    10201 cgtcgaaaag tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc caccaattac
    10261 ttcttcgatc gtaggaacaa aatgagtgac tgcgcggtag attgggaaat aatttgttcg
    10321 gatgttttct ttcaatggat tcatgacttg gatatgattc ttgattctta caatcagtgc
    10381 agccatgtgc ttatagagat tttcgaataa ggcttcttcc tttagatgga aaggtacgtg
    10441 tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt gtacccactc
    10501 gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa gataatttct
    10561 ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga
    10621 aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa attcatgttt
    10681 cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat aaatatcatc
    10741 ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt cctccaaagg
    10801 aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat tcataaagaa
    10861 gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga tagatcgttc
    10921 tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc cataactgac
    10981 cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga tttgaaattc
    11041 ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta aaactagatc
    11101 aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt cagtttctaa
    11161 tcgagcttgc ttttcctttt ccagcttcaa caaaaaacct ttgttgcgaa ttgtacggat
    11221 ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct gacgaatcgt
    11281 ccgctgactg acattgaact cacttgctag atgttttgct tttactggat attgggctgt
    11341 catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct ccttctgatt
    11401 tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact tgaagtggaa
    11461 agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa
    11521 agacatacgc ctattattac aaaaattttg cccatctata caaaaaagag gttgtgaaaa
    11581 agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc tattttttgt
    11641 ttggttgttc ttattatcgc agatgcaagc ttttaaacac catttagtgg agaagctgtg
    11701 acagagatgt tcagccttga gcgataagaa gcgattttga aaaatcgctt cccatatttt
    11761 acaaaatcgt gccttattgc cgataggcta tctctggaac accgtttatt cagaaaaagg
    11821 agctgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca atccagcata
    11881 atcggttgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc aaaataccat
    11941 atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc atcttttcga
    12001 tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt ttcgtggatc
    12061 tgtcgataat ttcctacttc ctgaatagtt atttttttag tatgatttct tacaccgtct
    12121 gctttgtttt tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt ataacagctt
    12181 ctggcttttt caggagtttc ttcttcccaa gcggattgga tcttcatgaa tatcttttgg
    12241 atttccaaaa actgtgcttt cttttcttta gccgttcctg acatgcggga caatatctcc
    12301 gatttcagcc ttttcttttg tcttttattc ttgacgatag aaaaagcaga aacccctcct
    12361 actgacaaaa cagctaatgc aagaaacccc gtttgctcat aactagaagg acctgtactt
    12421 ggaccataat acccataaga atctctgccg taagaacgac tgccaaaata actgccccca
    12481 tatgaacgac cgccaaatga acctctgccc gaacttcgac cgctagatgt acctccacca
    12541 tgaccacccg cgacagcgaa tccttgggtc ggcagagcaa acaaacatag gcccactaac
    12601 aaaatgattg ctaaataatt gattttttca ttgtcttcct cgttatcttt tattacaaaa
    12661 aactattttt atcatagcct tgctaaaagg aagagtcaat aaccagatgc ataaactgtt
    12721 tttttagcaa ttttgtctta tttagtcatt gagtaacatc ggacagatgt atgtgtcggc
    12781 agtccataat tcctctatct tcagcatgaa caagcttgca gacaagagat ctgcgcttcc
    12841 tccaggagag atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg
    12901 taaagcactt tgactagcag catgagcaag tttttgcatt tccactaacg ttttctgatc
    12961 actgcgatgc aatacgcatg tgtcatctag cgaactcatc aaaactaaca acatatgcat
    13021 ttgtttctgt acatatgaat cattatctag ctccttagct gcttggattg caagcctgat
    13081 atgcggatac cctaatatcg cttcttcata tgcaccattt acgccatatt tttgcttcac
    13141 cctatgacca tgagtggttt ttttatgacg atattttgta tcaggaaaag cagcaagctt
    13201 gctgacatca tcaagtattt tcgataaaga aaaaggctgt tccatactga ttttttgtgc
    13261 aatgacactg atcaatagcc ccattgccca aatcgctcct ttatgggtat tgatgccatt
    13321 tgtagcaatc agcattgcct tttccgcttt tcgcccaatc tcagcaattc tttctcttaa
    13381 ctcctgatca atagcataat gccatgctat ttgagccatt tcttcaaaaa aaggagttag
    13441 cgtcttagca gacttatcca aaatgcgccg accttctttt acggcttttg cgatcgtatg
    13501 gaaattatca tctcctaaaa ccatatcagc tgcctgtttg cttacttcac tacctttgat
    13561 tcccatagcg attcctatat cggctctttt caaagcaggt gcatcattta cgccgtctcc
    13621 ggtcattccg acgatttctc catttttctg caaagctttc acgattcgca gtttgtgttc
    13681 tggtgtcgtt cgagcaaaga cgtcgacttt tggtacttgt cgaatcaact cttcttcaga
    13741 cataagatcc aaatcgatcc cttctaaaac tttagcagta tgttttaagc caatttgttc
    13801 cgcgatcgct tttgctgtat ctttgtgatc ccccgtaatc attttcactg agattcctgc
    13861 ttgttggcat tcctttacag ctttgactgc actttctttt ggcggatcaa tcaacccagc
    13921 aatccctgca aaagttaatt gggttaaaga ctcatgcgtg agttcctgtt tcgaatctac
    13981 ttttttgtaa gcaaatccta aaacacgctg accttttttc gcaaacgctg tggctgtttg
    14041 accccatgct tcttattctg aagaagaaag agttgattta gccaataata cttctggcgc
    14101 acctttgacg taaatcaccc ctcctttttc ctccgcatgc atcgttgcca tatatttgta
    14161 ttcagagctg aacggaattt ttgcttctat ttttttcaaa ggcagatcgg ctgtttcggt
    14221 gtggcgaatc agtgcaagtt ctgtcggatt gccacttaaa ttttctgttt tctgttcttc
    14281 tttcgtcttt aattcctgac aatttttcat aataaacagt acgcgagttt cttcctttgc
    14341 tgctacatcc acaactgtca tttcattttt tgtcaatgtc cctgtcttat ctgaacagat
    14401 cactgtcata gaacctaatg tttctacaga aggcagtcct ttgatgatcg ccttttcttt
    14461 tgccatttca tgtactccga cagataggat catggtcaac acagctggca atccttcggg
    14521 taccattgac accaccaaag cgatcatagc tgaaagaagg atatgccaat ccatgccata
    14581 gcggaatgtt gtaaaaacaa ctaagaacaa gatcaaacaa agaatgcctt gaaaaatttg
    14641 tttgttcaat cggttgatct tcttgatcag aggcgtcgtt tgttgttcga ccgattgcag
    14701 tgcttgattg attttaccga tttctgtcgt gtttcctgtt tctacaacga caccgactgc
    14761 agaaccagat tgtacaagcg ttcctgaaaa agccatattt ttttggtcac cgattgattc
    14821 ttctgctgcc aaaacggaat gatttttttc agatgcttct gattctcccg tcaaaatcga
    14881 ttcttctact agtagatcat gtacttcgat caaccgaaca tctgctggaa cgacgtctcc
    14941 ggcacgtaag acaatgacat ctccttttac tagttctttc gcagggattt tttcttgcat
    15001 cccgttcgat agtactatcg cttcttgtcc catcagctgc ttcaaaccat tcaacgattc
    15061 ttctgctttt ctttcttgcc aataactaac gagtccgttg acgatcacta cgaataaaat
    15121 aatacttcct tcgatgtatt cgctcgaagc aaatttcaaa aaagaggcaa tgatcaacac
    15181 gatcatcagc agatcagtaa agtgttttgc tatttttttc catcgttttg tctgctgtct
    15241 tgcttctatc tcattatatc cttgatcagt caggcgtttc tgccgttctt cttgcgacaa
    15301 gccttctgtt gatgtttgat agtttttcaa tacatcttct accgtcaatc tgtaccagtt
    15361 catcctatcc cctcctgttt aattgatact gttattatac gcagggaagt tttttcaggc
    15421 gtatcgatta tttcaggggg gtctgaaata attcgttttg cagaatgtat ctgggcctaa
    15481 ctgatatttc tattgcttat tcttttatat acaaaattta agaatacaaa ccagataacc
    15541 aataaaaaag gaataaagcg tgtcgtgctt tcgaataaca agctgaaagc aattgaagta
    15601 aaaaacacca acaaagcaag atctttatat ctttgtgtag tgttccgttt cttttcttta
    15661 attaattgaa catgtgcaca caagatcgaa gaccacacaa atagaaaaca actcgttgtc
    15721 atgcctgcca aaagctgaaa aaacaatcga ctatcagcta caaaaaaatg tagacaaaga
    15781 ccgacaaaaa ataacatccc tgtaattaaa ataccgtgta aaggtacgcc tttcttagaa
    15841 agcgcacaaa accaatgggg caaacttttc tcttttgctg tttgatataa aatcctgcta
    15901 gtactgtaga ctgcactatt tgctgaggac aaagaagcaa ccaaaatcat tagattgaca
    15961 attgctgctc cgctttggca atggatcact tgcagcatag tcactaaagg actttctttt
    16021 aaatctaaac aatgccatgg aacaacaaat aaaatcgaca aaatcgttcc tatatagaaa
    16081 aagaggatac gccaaggcag acagttgatt gcatttttca aattcttttg tgggtcactc
    16141 gtctcaccaa ctgttaatcc gatcatttca attccagcaa aactgaataa aaccatagga
    16201 aatgaagcaa tcattccagc aaagccaaac gggactaatt cattgagctg tccaaaattt
    16261 gctttgattc caatagactg ggtatgaaaa tctgagacac tcaatcctat acccaataag
    16321 ataaaaagga aaatccccaa gatcttgatg attgagaaaa aagattccaa ttcaccaaac
    16381 cacttgactg caacaaggtt gactgccatt aagatcatta gtgcgctacc tccgcttatc
    16441 aaagctggta catgtacaaa ccagaaatgg atatacaatc ctattgctgt taattccgca
    16501 agtccagctc caagccagca aagccaataa gtccaattga caaaaaatgc ccactttttg
    16561 cctaaataat agtcaacgaa atcactgata gagttaaatg tactatttgc taaaaataaa
    16621 tcacccatga cccgcatcaa accataaata gccaaaccgc atatcaaata aacgagaata
    16681 acagctggcc ctgctgatgc aattgccttt gctgaaccta aaaataatcc tgttccaatt
    16741 gcgccaccta atgcgattaa ctggatattt cgattagtta gagtccgttt cattgccggt
    16801 ttttccatca agttcacctt tttctttttc aagcagcatg gcgacttcct gttcccaaga
    16861 aggtttcttt attttttcaa tacagagtgg tactacaaac aaaatgacac tgatggctgt
    16921 gactgcaatt agattgatcc atgtcccagc tttcatcgtc agtccgacag aaaatccgat
    16981 tgccgcaaat aaaacaataa cgacagtcca aactccaaga ttgctctttt tctctttttt
    17041 tccaattcta aatggtcgat tcaactttgc atgtgtataa cgcaatctca aaactgcgat
    17101 ccccatgatg acatagacga tacagtagat aacagtagtt gaatttacga gcatcaaaaa
    17161 tgcttgattg acgccaggaa tcagcaaata aaaaagtgca aagattgaga taatgacaga
    17221 ctgagtcaaa ataacggatt tggatacgtc aaattgattc gtcttccaaa aattgaattt
    17281 tggtggatac gcaccttttc tagcactttc tgtcactgtc tttgctggtc ctgaggccca
    17341 agcagataat tggactgcta caccaatgaa gacaagtaaa ctaaacagat tcacaataag
    17401 atgtggcaaa ccaagaattt ggcaataaat cgaaatagat tgcgcgatat tattcaattc
    17461 catttgtcct tttggtacga catttgctac aactaaagca ttcaaaatat tgacgaaaaa
    17521 tataaaaatt aacgcggcaa atactccgcg aatatacgtt ttaactggat ttttcaaccg
    17581 agtgatatac acagaggaca tttcgatacc agtaaaaata aacatgatcg gcgcaaaata
    17641 aacaaatgag gatgcagtcg tagtatctgg tattaatttg tcccatgaaa aactgcctaa
    17701 tgttccggtt ggtacgatcc caactttgat ccacgcagca aaccctaata agagcatcat
    17761 cgtcaaaggc acgtacaatc ctaaccaaac accgactttt ccgccgattt ttgccatatc
    17821 aaatttcaaa ttcaaaaaag tgatgatcca ataaacgact aaaatgacaa ctaatgtaaa
    17881 tttgctgttc aaaccaagcg gcacattatc gataatatta ccaaacaacg gagcaagtgc
    17941 tgaagcgacc atcaccatcc caggaaacat ttggacccat aacagccatg agacaacgaa
    18001 cccccatttt tttcctagag cattcgatac ccaaagttct ggaccacctt tttggggaaa
    18061 cattccggca aattcaccag agatcaatga gattggaaaa gcgtacaata atactgcaac
    18121 cagcatgtaa aaaatcatcg tccagctcgt tgctgctaca tctggaatat ttcgtacact
    18181 tgctacaagg gcgcaggtca ttccgataaa ggtcatggtc gtgatttgct ttgatgaacc
    18241 agtatctttc atctttattc cttctttcaa tagcgacata tgtttttgtt ttcttttatt
    18301 tttgttcaag aacagaaatg acaaggtttc atcgattcag tacaaaaccc agtatcaccg
    18361 aaacatccat tgaacgatct tagtgaactg ggtttgatac atgaaacgaa cgatccaact
    18421 tatagaacgt tttataaaaa agaatatctt ggcgaggaat atatgaaaaa cagggggaaa
    18481 tacaattgtt ttgatgaagc aaataattgt ctgttctcca tatgtctctt gttattcttt
    18541 cacacaaaaa tcagttctat tggatgattt cgtttagttt cttttccatt gctttggcga
    18601 tttcttttgt ataaaaattg aaaattcgat catcattcaa ataaggagtc atgatagccg
    18661 cacgtagcag agtgattttc tgttcttttt tccattcttc tttcgttaga cccattcttt
    18721 caacaaaacg aatcggagaa tcaccgtaat cttcttgtgt aaatgtagta tgagaagtaa
    18781 taaaccgttc accataaaca tcgccatcca tataagaaga ccgatcgaac attttttcat
    18841 ttaattcatt gatagcgttc aaatctgtac agccttgttc tttgaatacc caattgacca
    18901 tattaaaatc aggatgattc aatggataga cttcgattgt ttttccttta acagtaaaag
    18961 tcagatgatc gagaaattct ctgaatcttt gagctgcctc gattgaagcc ccaatcaatt
    19021 gaccatatcc ggtcacattc aatggcaata cacgatgtgc tgtccagact gcggcggcgg
    19081 ttgctccagc ttttgatcct tccaaaatat aagctcctaa catatctgga gcctttactg
    19141 atttttcaaa cacatatggc gcgaaataag aaatgatatt tctcatattt ttatgtttga
    19201 ttacgattcc tccagcagca taagggacat agcccatctt atgaggatct atcgtgacag
    19261 aatccgcttc tgaaatagct ctgaaccctt catatacctc tttgtcaatc gttacgcagt
    19321 ggtggaaaac atgatgttct tcaaagaatt cagctaagga agcatacggc acaaattctc
    19381 ctgcttcgtt tagaaacagt gaacgcgcat agccgccata tgcggcatca acatgtaaat
    19441 agaaatagat cccttcatct tttaaccttt ctcttaattg gacgatttta tctacactgt
    19501 ctacttgacc ttcttctgtc gttcctacga cagcaaccac acctagaatt gggatttttt
    19561 gttcagctaa ttcacgaatt gttttttcca aagcattaat atccattcga taatcctctt
    19621 gaactgggat agcaaccatc tgatctaagc ctactccgca aatatctaac gccttcatcc
    19681 aagaatagtg ttttgtttga ggcaccagcc atttccctaa tctttggata ttttttccgc
    19741 tacgggaaga agctgctttg acctcatcca tttcttcgtc cgtaaagctt tcggtcattt
    19801 ccaaaatttc ttcaacagat aaattcaaaa gttcccactc agacattttt ttcacttgtt
    19861 caggcagcac ttccttgaca gctaaaggaa tcgatttgat gcagcgggcg taccataatc
    19921 cttcaagatt agcaatagaa ccatctgctg caatgtgtcc ccatccgtct gtcatattga
    19981 ataatgaagc aaaatcctgt cctacttctg cttccatttg cgaagttgcc atagaagatt
    20041 ccaatgcaac attatttggg ttccacagca tcgcatagtt gtaagctaat aaagcaggca
    20101 tcaatgtttc agcattcatt tgtccccagt aacggcctgc tgaatgccat gggatcgatc
    20161 ctgcgcgtaa tcgctgactg acttcatcca agacctctat catatggtcg cgggtggcta
    20221 gataatctgg agtgagctta tctccttcgc taattgcagg catatctgaa ggaatataat
    20281 tttctctcca tccaagatgt tcatctacca tcttgttcaa caacatttta taaactggtc
    20341 cattttcagc tttgtcgcct ataaagacgg ccttgatatc catatctttc atttttcttc
    20401 cctccatatt ttgttggtca atgtctcgca gatacatgtg tttatctgta acacctcttc
    20461 actatagcgc caaaaatgag aaattttttt ttataacatc aaatccacgt agatttttac
    20521 a
//
LOCUS       DACEZN010000037        20359 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEZN010000037 DACEZN010000000
VERSION     DACEZN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.037"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            291..1430
                     /locus_tag="IXH60_001027"
     CDS             291..1430
                     /locus_tag="IXH60_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1989982.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="IXH60_001028"
     CDS             complement(1497..2072)
                     /locus_tag="IXH60_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1989983.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="IXH60_001029"
     CDS             complement(2077..3051)
                     /locus_tag="IXH60_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1989984.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="IXH60_001030"
     CDS             3334..4302
                     /locus_tag="IXH60_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1989985.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="IXH60_001031"
     CDS             4415..4693
                     /locus_tag="IXH60_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1989986.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="IXH60_001032"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="IXH60_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1989987.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5159..5905
                     /locus_tag="IXH60_001033"
     CDS             5159..5905
                     /locus_tag="IXH60_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1989988.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="IXH60_001034"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="IXH60_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1989989.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="IXH60_001035"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="IXH60_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1989990.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="IXH60_001036"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="IXH60_001036"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1989991.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="IXH60_001037"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="IXH60_001037"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1989992.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="IXH60_001038"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="IXH60_001038"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1989993.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="IXH60_001039"
     CDS             10642..11184
                     /locus_tag="IXH60_001039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1989994.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="IXH60_001040"
     CDS             11207..12763
                     /locus_tag="IXH60_001040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1989995.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="IXH60_001041"
     CDS             12777..13679
                     /locus_tag="IXH60_001041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1989996.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="IXH60_001042"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="IXH60_001042"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1989997.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="IXH60_001043"
     CDS             15122..15544
                     /locus_tag="IXH60_001043"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1989998.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="IXH60_001044"
     CDS             16013..16243
                     /locus_tag="IXH60_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1989999.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="IXH60_001045"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="IXH60_001045"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1990000.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="IXH60_001046"
     CDS             17654..17830
                     /locus_tag="IXH60_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1990001.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="IXH60_001047"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="IXH60_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1990002.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="IXH60_001048"
     CDS             18325..18708
                     /locus_tag="IXH60_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1990003.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="IXH60_001049"
     CDS             18877..19761
                     /locus_tag="IXH60_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1990004.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19878..20111
                     /locus_tag="IXH60_001050"
     CDS             19878..20111
                     /locus_tag="IXH60_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1990005.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="IXH60_001051"
     tRNA            20199..20279
                     /locus_tag="IXH60_001051"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="IXH60_001052"
     tRNA            20286..20357
                     /locus_tag="IXH60_001052"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5609 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagta
//
LOCUS       DACEZN010000038        19690 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEZN010000038 DACEZN010000000
VERSION     DACEZN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.038"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(140..382)
                     /gene="acpP"
                     /locus_tag="IXH60_001053"
     CDS             complement(140..382)
                     /gene="acpP"
                     /locus_tag="IXH60_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1990006.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(504..1505)
                     /gene="plsX"
                     /locus_tag="IXH60_001054"
     CDS             complement(504..1505)
                     /gene="plsX"
                     /locus_tag="IXH60_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1990007.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1554..3590)
                     /gene="recG"
                     /locus_tag="IXH60_001055"
     CDS             complement(1554..3590)
                     /gene="recG"
                     /locus_tag="IXH60_001055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1990008.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3707..5389)
                     /locus_tag="IXH60_001056"
     CDS             complement(3707..5389)
                     /locus_tag="IXH60_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1990009.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5441..5803)
                     /locus_tag="IXH60_001057"
     CDS             complement(5441..5803)
                     /locus_tag="IXH60_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1990010.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6073..6261
                     /locus_tag="IXH60_001058"
     CDS             6073..6261
                     /locus_tag="IXH60_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1990011.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6499..9168
                     /locus_tag="IXH60_001059"
     CDS             6499..9168
                     /locus_tag="IXH60_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1990012.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9501..10247)
                     /gene="truA"
                     /locus_tag="IXH60_001060"
     CDS             complement(9501..10247)
                     /gene="truA"
                     /locus_tag="IXH60_001060"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1990013.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(10450..11247)
                     /locus_tag="IXH60_001061"
     CDS             complement(10450..11247)
                     /locus_tag="IXH60_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1990014.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            complement(11237..12106)
                     /locus_tag="IXH60_001062"
     CDS             complement(11237..12106)
                     /locus_tag="IXH60_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1990015.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            complement(12082..12921)
                     /locus_tag="IXH60_001063"
     CDS             complement(12082..12921)
                     /locus_tag="IXH60_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1990016.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            complement(13193..13573)
                     /gene="rplQ"
                     /locus_tag="IXH60_001064"
     CDS             complement(13193..13573)
                     /gene="rplQ"
                     /locus_tag="IXH60_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1990017.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            complement(13601..14539)
                     /locus_tag="IXH60_001065"
     CDS             complement(13601..14539)
                     /locus_tag="IXH60_001065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1990018.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            complement(14619..15008)
                     /gene="rpsK"
                     /locus_tag="IXH60_001066"
     CDS             complement(14619..15008)
                     /gene="rpsK"
                     /locus_tag="IXH60_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1990019.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(15036..15401)
                     /gene="rpsM"
                     /locus_tag="IXH60_001067"
     CDS             complement(15036..15401)
                     /gene="rpsM"
                     /locus_tag="IXH60_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1990020.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            complement(15419..15535)
                     /gene="rpmJ"
                     /locus_tag="IXH60_001068"
     CDS             complement(15419..15535)
                     /gene="rpmJ"
                     /locus_tag="IXH60_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1990021.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            complement(15568..15786)
                     /gene="infA"
                     /locus_tag="IXH60_001069"
     CDS             complement(15568..15786)
                     /gene="infA"
                     /locus_tag="IXH60_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1990022.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            complement(15977..16624)
                     /locus_tag="IXH60_001070"
     CDS             complement(15977..16624)
                     /locus_tag="IXH60_001070"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1990023.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            complement(16686..17981)
                     /gene="secY"
                     /locus_tag="IXH60_001071"
     CDS             complement(16686..17981)
                     /gene="secY"
                     /locus_tag="IXH60_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1990024.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            complement(17981..18421)
                     /gene="rplO"
                     /locus_tag="IXH60_001072"
     CDS             complement(17981..18421)
                     /gene="rplO"
                     /locus_tag="IXH60_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1990025.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            complement(18465..18644)
                     /gene="rpmD"
                     /locus_tag="IXH60_001073"
     CDS             complement(18465..18644)
                     /gene="rpmD"
                     /locus_tag="IXH60_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1990026.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            complement(18659..19159)
                     /gene="rpsE"
                     /locus_tag="IXH60_001074"
     CDS             complement(18659..19159)
                     /gene="rpsE"
                     /locus_tag="IXH60_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1990027.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            complement(19180..19536)
                     /gene="rplR"
                     /locus_tag="IXH60_001075"
     CDS             complement(19180..19536)
                     /gene="rplR"
                     /locus_tag="IXH60_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1990028.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
BASE COUNT         5443 a         4310 c         3531 g         6406 t
ORIGIN      
        1 aaaccgcttt actttgcaac ataatttctg tttatcgcca gaattatcag aaaaatgact
       61 tttacgtgtc aaaaagatta aaaaaagaag gataaaaagc tgcatgcagc tttttatcct
      121 tctttgcttt tatttgaaat tatttaagat ggctgctgat ataatctact gcgccaccga
      181 ctgtttcaat ttgttcagcg tcttcatctg aaatttccgt accgaattca tcttcaagct
      241 ctaaaacgaa ttccataata ctaattgaat cagcttttaa atcatctttg atgttcaatt
      301 catccgtcac tttatcggta tctatctcaa aatgatttga aataattttc gctactttgt
      361 taaatacttc ttcgcgagtc aactttttca cctccaagaa tcatacactt gtcaattacc
      421 tacaccatgg ctaattttac tggcattttt caggaatgtc cacctttttt tcaatggaac
      481 ggatcgcttc agatatccaa atactattct tcgctttttt caaattgttc gactaattgt
      541 ccaactacat ctgtctcaag cattgtgtgg atttggcgga tcgtatagcg aacagcatcg
      601 gggcctgttg ctccatgagt tttaatgact ggtgctttca agccaaacaa aacggcaccg
      661 ccatgtttgg aataatccat ttctgctttc aaagaacgta agccgtcttt taaaagcata
      721 gcgcccattt ttcctttgat cccttcattt agaatagcag attttaatag attcatcata
      781 ttcattgctg ttccttcaat agatttcaaa acagcgttgc ctgtaaatcc atctgtgacc
      841 actacatcag ccacaccatt gagtagctca cgagcttcta cgtttccaat aaaattcaag
      901 cttgtttcat tctgaaggag ttcataggct tttttcgtca cttcactacc ttttgttgct
      961 tctgttccgt tgtttaagag tgcgacacga ggacgtccga tacctcggac tttacgtgca
     1021 taaaaagaac ctaaaatacc gtattgcaac aaatgttctg gtttgttttc tgcgttagcg
     1081 ccaagatcca gcatatcgaa accggcacct tctttcccta ctactggcaa ggtagacatc
     1141 aagccagggc gttcgatatt tttgatacgt cctacgatga acaaccctgc tgctagtagc
     1201 gcgcctgtat tccctgctga aaatacagca tctgcttcac cgtttttcac tgcttgtgca
     1261 gcaaggacca ttgatgcagt cttttttcgt cgaattgctt ttactggctc atcatcacta
     1321 ttgatttttt cgtctgtatg gataatcgtg atgttttttt catttgttaa gtatttcttg
     1381 atttctgctt cttttccgta aagtaagaat tctacttctg gaaaatcttg ctgcgctaaa
     1441 gccacgcctt ctacaattgc ttgaggtgcg tgatctccgc ccattgcatc tacggcaatt
     1501 ttcattgttt ccactccttt ttccgtcatc agtataaagt atagcacagt ctgctaatcg
     1561 aaataacgat tatcctgatt ttctatttgg aggctttgcg ctaaaggctg atactcagga
     1621 agcatctgcc aatcctttat ttgccaaagg gctgcagcct cttctctggc tacctccaaa
     1681 acatttccat ccgtcaccaa gtcagcaatc gcaaattgag gcagaccaga ttgacggaag
     1741 ccaaatactt caccaggacc acgtaattcc agatcttttt cgcttagtac aaaaccattg
     1801 gtggtctctg tcatgatttt catccgttcc acacctagtt catttttagg attagcaact
     1861 aagatacagt aggaagcatc acttccgcgt ccaacacgtc cacgaagttg gtgcagctgt
     1921 gccaatccaa atcgatccgc atccatgatc agcatgacgg tcgcgttcgg cacgtttacc
     1981 cctacttcaa ttacagtagt cgaaactaat atctgcatct ggttgtcttt gaaggcttcc
     2041 atgattactt ctttttcctg attcttcatt ttaccatgta acaagccaac ttcatatttt
     2101 ggtgcaaata gttcacgaag tttttcatag atttccactg cattttttac atctaatgct
     2161 tctgattctt caatcagcgg acagatgaca tacatttgat gtcctcttgc cagttctttt
     2221 ttcgtccaat ccagtacact gtccaattga ggtgttctta cccagcgtgt ttcgatcggg
     2281 atacgtccag ctggaagttc atcaatgata gatacatcca tctcaccata tgctgtaata
     2341 gctaatgttc taggaatcgg agtcgctgtc ataaataaga catctggtcg ccagcctttt
     2401 tcacgaagta ctttccgctg gttcacgcca aaacggtgct gctcatccgt aataactaga
     2461 cctaaattct gaaattcgac tccatcttgg atcaatgcat gtgtgccgat cacgacgtta
     2521 atttcccctt gctctagttt ttcaaggagc tcacgtcgtt cttttgcttt cgttgacccc
     2581 gtaatcaatg cgatggttac ttcattggga tcaaacagct gttgcaagct ttccatatgt
     2641 tgttgtgcca agatttctgt aggcaccatc aacgcacctt ggaaacctgc tgtaacggta
     2701 gcgtataacg caatcgcggc aaccactgtt tttccgctac ctacatctcc ctgcaaaagt
     2761 cgctgcatat gctgcggact gcgaagatcc cgacagattt cattcgttac acgtttttgt
     2821 gcatttgtca gttcaaaagg tagcttttga gtaaattgtt tcaatttttg gacatcataa
     2881 aggatctcca aaccattcgt ttctgctttt tctgcttttt tcaatccttg aagacgcatt
     2941 tgaaataaaa agaactcttc aaaaacgaca cgtcgtttgg cttgatggtt ttcttcgtgg
     3001 tcttttggaa aatgcatact tcttacagca gaagctcggt caagcagtcg atatttctcc
     3061 acaaggttgt ttggcagatt ttcttcaatc aatgtgccgt attcttgaaa cccttttttg
     3121 attagatcaa ctaatgtcgt ttgacgaatt gatttgttga cgtgatatat cggcgaaaag
     3181 tcatccattg tttgagaacc taaaatcttc atcccgttca aggctttacg ctttgcatcc
     3241 catttaccgt atacggcgat ttcctccgat aaaatgactt tttcttttag ataaggttga
     3301 ttaaaaaaag aaacgttgaa cacatcatgt tcttgcatca tacgaaattg caatcggctt
     3361 tttttataac caaatcgatt catcacagga ggcgagacta cgattccttt caaagtaacc
     3421 ttttcttgat cctggatctc gtttagattt ctttcctgaa tatcttcgta gcgaaaagga
     3481 taataagtca gtaagtcttc aatcgtttgg atgcccaagc tggcaagact agctgctctt
     3541 ttttctccga ctcctgccag catagtgatt ggatcatcta aagaacgcat aaatttttcc
     3601 tccctccttt aacaaaaaaa ggacttggtc agcgatcata cctgatcact aaccgaaggc
     3661 cttttatgaa tgaatactgt gttagaaaga tattctgaca cagaaattat tcagcagaaa
     3721 atagataagg atagacaggc tgatctcctt ggtggatctc gatttccaaa tcaggatgtt
     3781 tttctgttag tttctcagca aattcatttg cttgttcaac tgttccttca tcacctgcca
     3841 agatcgttac gatctctgtg tcctcattga tcatttgttc tagtgtactt aacgctgttg
     3901 ctgtgatatc tgggttagag atcaggattt ttccatcgat catgcccaaa aagtctcctt
     3961 ctttgatctc tacaccgtca attgaggtat ctctgatcgc gtgagtgact tgaccgctca
     4021 caacgctaga caacatttct gtcatcgttt ctttgttttc ttctaaagaa gcttgatcat
     4081 tgaatgaaag catagctgtc aatccttggg aaatcgttct tgaaggaaca actgctactg
     4141 ggatatctgc tacttctgct gcttgatcag ctgccatgaa gatgttctta ttgtttggca
     4201 agacaatcac tttatctgcg tttacttctt gaatcgcttt aacaatgtct tccgtacttg
     4261 gattcattgt ttgtccacca ctgataacat aattagcgcc taaactttca aacagctctt
     4321 ggacgccttt accagcagcg atcgctacga taccatacgg gatacgtggt atttcttttt
     4381 tcgcaaactc gttcacttct tcatcgtgtt ccaaaatcgt ctcatgctgc aagcgcatgt
     4441 tgtcgacttt gattttgact aatgaaccga atttttgtcc ataattcaag acttctcctg
     4501 ggttttctgt atgcacgtgg actttgatga tctcgtcatc attgaccaca agtaaagaat
     4561 ctcctatacc atctaaatag ttacggaatt cttcgtaatc gaatgtacta tcgactgtag
     4621 gtccttctcc gatttttacc atgatttctg tacagtaacc aaaatggata tcttctgtcg
     4681 ccatttgacc ttggacactg cgatgatgtt ctgcattgac catatcatcc atttctgctg
     4741 gagaaggttc atatgtttca tccgcttgga attctccatt caatgcactt aaaaagccct
     4801 cataaacaaa cagcagtcct tgccctccac tgtctacgac accgacttct tttaggactg
     4861 gaagcaagtc tggagtttta gctaaagcac gctttgctcc ttttacaaca gctgtcatta
     4921 cttcaacgac atcatcagta ctttttgctt tcttttctcc agctttcgct gcttctctag
     4981 ctactgtcaa aatcgttcct tctactggtt tcattactgc tttatacgca gtttcaactc
     5041 catgagtgaa ggctgcagca agatcagaag catttaatga gacaacatct ggaatttgct
     5101 tagaaaatcc acgaaacaat tgagacaaaa taacgccgga atttccgcgc gcacccatca
     5161 gcaatccttt tgaaagcatt gctgctaatt ctcctacttt ttctgaggtt gaatctacta
     5221 ctgccttagc tccactagtc atagatagat tcatatttgt tcccgtatcg ccatctggca
     5281 cagggaatac atttaatgaa ttgacgtatt ctgcatttac ttgcagacga ttcgcacctg
     5341 cctggaccat ttcctggaac tggcttgcac ttatttctgt tacactcacc tagataatcc
     5401 tccttcagat cgactgctga gttaccagcc agtctcctgc ctagtctggc agtacacgaa
     5461 caccttgtac aaagacatta actgaattgg cagttacgcc taataatgcc tctagattgt
     5521 atttcacttt ttcttggacg ttgcgggaaa cttcagagat tttcgtacca tagctgataa
     5581 tcgtataaac atctacagca ataccattat cttcttggcg gacaactact cctcttgagt
     5641 aattttcttt acgcaaaatc tcagtaatat tatctttaat ttgatttttg ctggccattc
     5701 ctacaacacc gaacacatct gtcgcggcgc caccaacaac tgttgcaatt acatcattgg
     5761 taatttcaat ggttccagct gacgtttgaa tttttacagc catgaatgag cctccttaac
     5821 gggtaaaaaa ctcccatttt tcttctatca tattttatca tagcgcaact tgaaataaaa
     5881 ggcagttacc caaagtactc aaaaaccttg caaaatttca ggtataacct tgtttaattt
     5941 acaattattc ttctcaattg tcaattgtat ctatgaaaat gtacttgcaa attcccattc
     6001 tatatgatat gttagtctgg tatgagctga attattctat tgctccaaat caaggcaaag
     6061 gaggaaatta ccatggcaaa agtttgttac tttacaggtc gcaagacaaa aagtggaaac
     6121 aaccgttcac acgctatgaa ctcaacaaaa cgtactgtta aacctaactt acaaaaagtt
     6181 cgcgttatgg tcgacggaaa acctaaaaaa gtttgggtgt caactcgtgc tttgaaatca
     6241 ggaaaagttg aacgcgttta attgaatgat acttggaccg ttcagatagt gaacggtttt
     6301 ttatttaata aaaattttta agggtgggac ttttgattcc tacccttttt ttgcgtttat
     6361 ttttgttata cgaacaaatt atgaagttat ttattctaaa ttttattttt ttgtgaaaac
     6421 tagactcttt ttaagcagag aaaaacaagg atatgataaa ctgaatacaa cttcatagaa
     6481 atttggtgga aaataaatat gaaaaaaaat ttaaagtcat tctttagaga aaacggacgc
     6541 tacgcagcag ctagtttcct gattccgttt ttgattttag cacttgttta tctaagtatc
     6601 ggaatctatc ctggaagcag cagaagtatt ttggcaagtg atgccttttc acagttttct
     6661 aattttcatg ccagcttccg aaatgttctt cttggaaagc aaagtatttt ttatacatgg
     6721 aatgcttctc taggattgaa ttatttatct ttaatttctt actatctagg cgggatcttc
     6781 acgccactgg tgttcttttt tccaaaccag atgatgccag atgcactgta tgttctgact
     6841 ttgcttaagg tcggctgtgc cggattggct ttttggtttc ttgcacgtac gttcaagatt
     6901 cctcgctggg ggcatgttgc acttagcgtc agttacgcat tgatctcatt tatcactgca
     6961 cattcggaga tcatcatgtg gctggatgca tttgtttatc ttccattagt gattttgggg
     7021 attcatcgtg ttatggatct taaacggccg aaacttttat ttatcagtta tttactcttg
     7081 tttttatcca gtttttatat gggttttatg attggcgtat tttctttctt atattttatc
     7141 gcccgtctat tgacaaactg gtcagaatat aaaaaaacga ttgttcctta tggtatcact
     7201 tctttactgg caggcggcgc ttcaatgatc attgtattgc cagcagtgct ggatcttcgg
     7261 tcaaatggcg aggcactgag tcagatcact acgttgaaaa cagaagcgac ttcttatctg
     7321 gatctaatca tgaaaaatat gattggtgtt tatgacacga caaaatacgg atcgattccg
     7381 ttcgtttacg caggtctgct accattagtc ctctgtctgt tttattttgc cagtcggaaa
     7441 gtaccgttga aaaataaatt tttgtacggc agtcttttcg ccatacttat cgcaagtttt
     7501 tatcttgttc cattgaattt gttctggcat ggtatgcatg caccgaatat gtttttattc
     7561 cgttatgcct atctgttctc tttcttagtg ctcctgcttg caggaaaagg atgggaaaag
     7621 atagaacaag acgatggtgg gctgttgtta ggaatcattt tggtcgtagc tgctctcttt
     7681 gcggtggcat ggggcatcaa acctgaagga agctacacct atgtgacgat gacttccttt
     7741 gtattgacag tggttttctt agggatttat gcttttgctg tgggatttta tcagcataaa
     7801 aaactttcat tcaatcatct gagtatattg ttattattga tcatgtctgc tgaagcactg
     7861 gtcaatacga acagtatgat ccatggcatt cttgatgatt ggaattatgc ttcccgcagt
     7921 ttatatactg atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc
     7981 gactcgtttt accgcttgga aaatctggat ccagtatctg caaacgattc gatcaactat
     8041 ggttacagtg gtatcagttt gttttcttct ataagaaatc gtaattcttc ctcttacctg
     8101 gataaattag gtttccgttc aagagggaca aatttgaata tccgttataa caataatact
     8161 ttgctgatgg atgggtttac aggaatcaaa tataacattt ctaaaaacga aagcacgttc
     8221 agtaaatatg gcttccgcca agctggtcaa agcggcgact atacactgta tcagaatgtc
     8281 aatgcattgc cactagcatt tactgcaccg ttgtccgtta atgatgttga gcagccagtg
     8341 acagataatt tgaccagtca gacaaacttg atcaatacat tgtctggtct aaatgaacag
     8401 tattttacat tctatacacc tactttgaag aatcagttga atacgacgat cacaaagaca
     8461 acgactggcg tcacttactc agaaacagca gataaccagc caaaagagct cacttgggaa
     8521 gtccatgtgc ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa ttttgcgcaa
     8581 ttagaaagtt ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac
     8641 atttctggtc aatactatga tcttggctat tatcctcaag agacaacagt gacatttact
     8701 gcaagttttt atgggacgaa agaaataagt ttcatggagc ctaaagtcgt tggtctagat
     8761 gttgtagcct atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc
     8821 actggcagaa aagcttctgg gactgtaaat accgctgaag attccatgtt ggtgacaact
     8881 attccttatg acaaaggatg gaaagctaaa attgatggga aaccagtcaa ggtcagcaat
     8941 ttcaaagacg cattcttgat gataaaagtt ccagcaggta aacatactgt cactttcagt
     9001 tacttgcctg aaggatttac gataggtgca atcctttttg tcctatgtat tgcattgttt
     9061 attctttatg tctggtacct tgaccgcaat cgcccctttt atggcacgat tgatccagca
     9121 atagaagaac aaccacgaaa cacacacaga agaagacgta gaaaataaag aaacgatgga
     9181 acctgttcag actgtccgca ccaagggcaa tcaaaacaac gttccatcgt tttttggtta
     9241 cagaaaaaat aggtaacaaa aagatgattc acttatcaac gcctcgtcca caagtcacga
     9301 tattcatcaa agatgagaag ctgttttttg aaattgctct ttgtgactca gagttaaatg
     9361 acaggctcac gacctctttc ttaaacggtg ttccagaagt agcttcttcg aaaataagcc
     9421 gaaattttgc aaaattttga gaagtaattt tcgtaaatac cttcttattt cttgaagctg
     9481 accacttcta tcataacctc ttattcatat ttcacttcat acaaataaag tccttcaggg
     9541 tgtgctgtag gtcctgctgc attacgatct tttttcgcaa taatatctgg gatactatct
     9601 tcaggcaatc ggccattgcc aattttcaaa agcgttccta ctaagatccg tatcatctta
     9661 tacaagaagc catttcccct aaatgtaaac aacaattcgt cttttgtttc atttacctcc
     9721 atcttagcct cgtagatcgt acgtatttta tcctctatcg agcttcctga tgcacaaaaa
     9781 gaggtgaaat cgtgagtccc taataaatca ggaagcgccc gctgaatttt ttccaaatca
     9841 agtgggtaag gaaaataact tgcataatgg cgtcgaaaag gactgcgtgg tttccctata
     9901 tccactcgaa attgatacgt cttttctttg accaaatatc ttgcgtggaa gtcttctgaa
     9961 acaatttcta cttgtcttac agcaatatcc tctggtgact gggtatctag cgcgaaacgc
    10021 attcgttcta acggacgctc ctctggataa tcaaaatgga ttacttgccc gatcgcatgt
    10081 actccggcat ctgtccttcc cgaaccaaag accgtgattt cttttccatt tgccattttt
    10141 tgtaatgttt tttctacttc ttcttgaaca gtacgcccat ttggctgttt ttggaatcca
    10201 ttaaaatttg ttccgtcgta cgcaatgatt gctttgtaac gtaccatgtt tttctctcct
    10261 ttgttcctaa aaggttatct ggaataatga tttttctata aaattttttc gttctttctt
    10321 ttcagataaa agaaagaggc ctgtgacctt agtctgccac agaccctaat atttcttgtc
    10381 atacactgct tgcatccttt cttgcaaaca gatcagcaag aaaagaatag atagcggccc
    10441 actgaacgtt tatcctctta aaaagatcag tagcacagtt aataatacaa aagcaatgat
    10501 aaccatcgtg tctctattat gccaatgcaa tacacgatat ttcgttcttc cttccccacc
    10561 tttgtagcct cttgcttcca tagcagtcgc aagatcttcc gcacggttga agctgctgac
    10621 aaataaagga atcagcaaag gaatgactgc tttcattttc tgaactaggt ttccttcacc
    10681 aaaatcaaca ccgcgagcgc gctgagcgtt catgatcttt tctgtttcgt ccatcaatgt
    10741 aggcacaaaa cgaagagcaa tcgacagcat caatgaaatt tcgtggactg ggaaccgtac
    10801 ggctttcaat ggacgaagca agtactcaat cgcatccgat aattccaaag gcggtgtagt
    10861 aagcgtgagt agagtcgaca taaaaatgat caacacgaat cgacagaaaa taaagaggcc
    10921 gttcatcaca ccaaattcag tgatatggaa aacaccccaa ctccaataag tttctccgcc
    10981 acttgtaaac aagacctgca aagctacagt gaagaggatt agccagatca atgggcgaat
    11041 gcctcggata aaaaatttca aatctacttt tgacaagaag acagaaagta atgtgaaaaa
    11101 tgccaacaaa aggtacgttt cccaattatt tgctaaaaaa atgattccga taaaataaaa
    11161 actggctaat aatttagctc ggggatccaa tcgatggatc aaggaatctc caggcatata
    11221 acgaccaaat atcaatttat tcatcatggg catttcctcc agcataatgg acgatagcgt
    11281 ctgctaattc ttcagctgtc aaaggcaagg tagcaaagtt catcccttta gccattaatt
    11341 cttctgcaaa ctcggtggca gtaggtacgc ctagttgttt ttcttttagc cattcaatgt
    11401 tttgaaagac ctcttgcggc tccccgctat taacgattcg tcccttttcc aacacataga
    11461 cataatctgc aaaattggct acatcatcca ttaaatgcgt aactaataca atagtgatct
    11521 gctgttcctt atgaagacgc caaaacatct ccatcatttc ttttctgccc tggggatcaa
    11581 gtcctgccgt tggttcatcc aatacaagta cttccggcct catggcaagc acaccagcga
    11641 ttgctactct acgcatttgc cctcctgaaa gttcaaatgg cgaacgttcc aaatagcttt
    11701 catccagccc aacttgttct aatgtttctt ttgctaggac tagtgcttct tcttcgctga
    11761 caccaaagtt tttaggaccg aaagcaatgt cttttgctac cgtttcttcg aatagctgtg
    11821 cttctggaaa ctgaaagacg atgccaactt tttttcggat gggctttaaa ttcttattat
    11881 ccgtatccgg ctgaatatct cgttctccaa tatggactgt accgcttgtt ggcttaacaa
    11941 gcgcattcaa atgctgaagt aaggttgatt tcccgcttcc ggtatgccct actaatgcag
    12001 tataactgtt ttctttgatc gtcatattga tgtcaaataa ggctctttgt tcaaatggtg
    12061 tattcggctg ataagtgaag tctacttgtt caaaacggat gtccataacc aatccaccat
    12121 cctttcttct gtcatatatt cactgggcac atcaacccca cgttctttca gtgcgctctt
    12181 cagtttttca ggaaatggta gatctagacc taagtcgatc agttcaggac cagcagaaaa
    12241 aatttctttt ggtgtacctt cacggacaag ttcgccttgt ctcatcacta gtatccgatt
    12301 ggcattagct gcttcatcaa tatcatgagt gatagatata acggtcaatt gactttcttc
    12361 tttgatcttc ttgatcgtag agatcacttc ttcacgacct tctggatcta gcatactggt
    12421 tgcttcatct aaaataatga tatcaggtcg taaggcaacc acgccggcaa tggcaacacg
    12481 ttgtttttgc cctcctgata aacgagcagg ctcgcgtgaa gcaaactcag ccattctaac
    12541 tttctcaagt gcatctttca cacgcactag catttcttct ctgggaatcc cttgattttc
    12601 aagaccaaaa gcaacgtcat cttctactgt cgaaccaaca aactgattgt ctggattttg
    12661 aaaaatcatt ccaaccatct gtcggatcgt ccaaatattt tcttcatcca atatttgatt
    12721 tcccactttc acgatccctg attctggcag aagcaatcca ttgatcgttt tggcaagtgt
    12781 cgattttcct gaaccattgt gtccaataat agcgatccac tcacctttat caattgtaaa
    12841 ggagacatct ttcaatgcgg ggcgaagatc atctggctgg tatttataat ttatcttttc
    12901 taattcaata attggctcca tgattactcc ttcttcatcc taaagttcct actaacacta
    12961 tcaaaaaaag acaaaatttt caattatttc tcgcttaaaa tgttttttta tgccttcaaa
    13021 catatctgtc atgacaggta attgaaggta taaaaaaaca ctttatgatg agtgcttact
    13081 ccccaagcga tattcgcttg aggagcagcg ctgagctaga ctcggaaaaa tatcttccaa
    13141 catcataaca ctctaaaaga atcatctata aagtgatgtt attagtgtga ttttagacaa
    13201 gttctaagac aaccattggt gcaccatcac cgcgtcttgg ttctgtcttc aaaatgcgtg
    13261 tgtatccgcc ttgacgttct gcgtaacgag gtgcaatatc gttgaacagt ttttgtaaag
    13321 ctgattcaat gacgatgtcc tcattttctt cacgcacgct ggcaacttca ttacgtacga
    13381 aagccgcagc ttgacgacgt gcatgcaaat ctccgcgttt acctaaagtg atcatttttt
    13441 ctgcagttga gcgaacttct ttcgcacgag cttcagtagt cacaatacgt tcgttgatta
    13501 ataaatcagt tgttaaatca cgcaacatcg ctttacgttg gctagatgtg cgtcctagtt
    13561 tacgataact cacgttggtt tccctccttc gtaaaaggtt ttaatcttct ttacgtaaac
    13621 ctaaacctaa gtcgtgcaat tttgctttaa cttcttctaa tgatttacgt cctaagttac
    13681 ggactttgat catttcaggc tcagatttat ttgtaagttc ttgtacagta ttgattcctg
    13741 cacgtttcaa acaattgtaa gagcgaacag acaagtctaa ctcttcgatc gtcatttcca
    13801 acattttttc tttttgtgtt tcttcttttt cgaccatgat ttcagcattt tttgcttcat
    13861 cagttaagtt cacaaaaata tccaaatgtt cagtcatgat tttcgcagac aaactcattg
    13921 cttccaacgg ttcaattgaa ccatctgtcc aaatctccat cgtcaattta tcaaaatctt
    13981 cacggtgtcc aacacgtgtg ttctcaactt gatagttcac acgacgaaca ggtgtgtaga
    14041 ttgaatcgac tggaagaacg ccaattggca tatcttcttt tttattttca tctgcttgaa
    14101 cataaccacg tccaggtttc actgttaagc gagcatggaa cgtagctcct tcagagacac
    14161 tacagatgta catatctttg ttaagaattt ctacatcact atcaacgata atatcgccag
    14221 cagttactgt agcaggtccg gtaatatcga tttcaagggt tttttcttct tggccataca
    14281 ttttcagcgc taagccttta atgttcaaga tgatttgagt gacgtcttct ctaacacctt
    14341 tgatggtaga gaattcatgc agtacaccat cgatttgaat attagtgatg gcagcacctg
    14401 gcaaagaaga taataaaata cgacgtaggg aattccctaa agtagtccca taccctcttt
    14461 caagaggttc aacgatgaac ttgccataat ctttttcttc atcaattttt gcgattcttg
    14521 gtttttcgaa ctcaatcatt cttatcttta cccctttcaa aacgaaaagt gtcttgttca
    14581 atgacgttac tgataaaact caaaatgagc ggcactcatt aaacacgacg gcgttttgga
    14641 gggcgggcac cattatgagg aactggagtc acgtcacgaa ttgcagtcac ttctaaacct
    14701 gtagcttgta atgaacgaat tgctgcttca cgtccagaac caggtccttt aactgttact
    14761 tcaacagtct ttaatccgtg ttccattgct gcttttgtag cagtttctgc tgccatttga
    14821 gcggcaaaag gagttgattt tttgcttcct ttgaaaccta atgaaccagc tgatgaccac
    14881 gctaatgcat ttccgtgagt atcagtgatc atgatgatag tgttattgaa tgtagaatgg
    14941 atatgtgcaa tacctgcttc tatattcttc ttcacacggc gtttacgatt cacttttttt
    15001 gctgccatga agttttaacc tccttcactt aagaattatt ttttcttgcc agcgatagcg
    15061 cgagctgggc ctttacgagt acgtgcattg tttttcgtgt tttgtccacg aacaggcaat
    15121 ccacggcgat gacggattcc acggtaagaa ccgatttcca tcaaacgttt gatgttcaag
    15181 ttcacttcac gacgaagatc cccttcaact tttaatttat ctacttctgc acggatagca
    15241 tctgtttgtt cattcgttaa ttcacgaacg cgaacatctt cagatacacc agcatcagct
    15301 aagatctttt tagcagtagt gttaccaata ccatagatat aagtaagtga aactactaca
    15361 cgtttatcac gaggaatatc tactcctgca atacgagcca tatttatagc acctccaatt
    15421 atccttgacg ttgtttatgt tttggatttg ctgggcaaat caccataacg cgtcctttac
    15481 gacgaattac tttacaatgt tcgcacattg gttttactga tggtcttact ttcatgataa
    15541 tacctcctgt agttttacgg agtacaatta tttaaagcga taagtaatac ggccacggtt
    15601 aagatcgtat ggagacaatt caaccgttac tttgtcacca ggtaagatac ggatgtaatg
    15661 catacgaatt ttacctgaaa cagtagcaag aacttggtgt ccgttttcta gttcaacttt
    15721 aaacattgca ttcggcaaag tttcgacgac tgtaccttcg acttcaatca tatcttcttt
    15781 tgccacgcac agtacctcct tgtacttgtt tcgcattttc acacgataaa atggcggaaa
    15841 ctgcagttcc cacctcaaaa ttctcaaaat ggatcatcta gaggattctc tagaagtcgg
    15901 actgacatat tctatcataa tttattgaac ttgacaagag taactgcata atgcaagtcg
    15961 gctacgggca atcgtgctat ttttcaatga ttttttgtac atctgcaaat acagcatcga
    16021 tttcacgagc accatcaata gtgtttagca aaccttgttc tttataataa gccaagattg
    16081 gttcactatt tttgatgttg atagccagac gatttttaac cgtctcaggt ttatcatctt
    16141 ctctttgata gaattcatgc ccaccgcaac gatcacatgt gtcttcaact tttgtagggt
    16201 tgaaaatttt atggtaagtt gctccacaat tgctgcagat aaatcggccg gctagacgtt
    16261 cgaccaagat ttcttcacca acatggatat cgatgacagc atcaattttt ttattcaaat
    16321 cttttagcat tgcgtccaga gcttttgctt gatctagcgt acgtggaaag ccatctaata
    16381 agaagccttt ctctgtatct ggttcagcta gtcgctcttt cacgattcca tttgttactt
    16441 catcaggaac taatgcacct ttatccatat aagactttgc ttccaggcca agagctgttt
    16501 cgttttgcat cgctgcgcgg aacatatctc ctgttgagat gtgcggaata ccataagcat
    16561 caatgatttt ttcagcttgt gttcctttac ctgcgcctgg cagccccatt aaaatgaggt
    16621 tcattgtttt ccctcctaat aaatgtgtga gaacgtgtgt tgaggggaaa cctcaacacg
    16681 ctttcttact caatgaatcc aacatattta cgtttcagca ttaagccttc taactgtttc
    16741 gcagtttcta aagctacacc gataacgatc agtaagcttg taccgcctaa tccgattgat
    16801 tgtggaagat cccaaaccat ttgagcaatg attggaagca atgccactaa accaaggaag
    16861 actgcaccaa ctgtacttaa acgcatcagt aatcttgaga catactcttc agtcccctta
    16921 ccagggcgta cgcttggtat ataacttcct tgcttttgca agttttccgc taatttctca
    16981 gggtttactt gaacaaaggc gtagaagaaa gtgaaggcaa cgatcagaat cgtataaatc
    17041 gtagcaccag gtgctgtatt atagttaaag atctccatca tcactttgta ccagttttca
    17101 gaaccgaatc taccgctcaa tgcttgtaaa acagcatttg gcgtagcaat aaacgaacta
    17161 gcaaagataa ctggaataac tccagctgca tttactttca acggcagata gctgcttgta
    17221 ggcgcgccag ctacgcgttt cgtatattgg atcgggatct tgcgttcagc ttgttggaaa
    17281 aatgttacga atgtcacgat caacaagacc gctactagta aaatggccac aaaaatagca
    17341 gatttccata tgtctgaagg atcaatattt ataaagtaat cttcataaat ttctctgata
    17401 ctttcaggca atctagaaat gatacctgca aaaatgatca ttgatacgcc atttccgata
    17461 cctttttctg tgatctgttc tcctagccaa gtaacaaaca tcgtaccggc tgttagaatc
    17521 aaaccgatcg ttacgtaagt tgctacgtta ggattattca cgaaacctaa ttgagcgtat
    17581 tgattgaatc cagcagtgat accgacagat tggataaatg ccaatactaa tgtcagatat
    17641 cgagtcgctt gattcagttt tttacgacca acttcccctt gtttcgacca ttcaacaaat
    17701 cgaggcacga tgtccatttg aagaagctga atgacgatag aagcagtaat gtatggtgaa
    17761 acccccattg agaaaatgga aaagttttgc atcgcactac cacttaccaa gttcaacatg
    17821 ttgaaaaatg gcaaagaaga aaggttttgc aatgaagcag catttacgcc tggtacagta
    17881 atatgtgcac ctaggcgaaa tacaaacaac acaaatacag taaataggat tttcgatcta
    17941 atgtctttta ctttaaaagc atcttttaat agtctaaaca ttagatcacc tcgattgatc
    18001 caccagccgc ttcaattgct tcttgagctg ctttagagaa ttttgctgct ttcacagtta
    18061 atttcttgtt tagttcccca ttagctaata ctttgattcc agctttttcg tttttcacga
    18121 ttccagcttc aactaaagca acaggagtta cttcagctcc gtcttcaaag cggttcaata
    18181 catcaagatt aatgactgca tagtctttac ggttcacgtt tgtgaatcct cgttttggta
    18241 aacgacggaa caatggtgtt tgtccccctt caaatcctaa acgcacaccg ccgcctgaac
    18301 gagctttttg tcctttttgt ccgcgtccgg cagtttttcc gttaccagat gaagtaccac
    18361 gaccaacacg gttacgtact tggcgtgaac cttcagcagg ttttaattca tgaagtttca
    18421 taggtttggc acctccttaa tgttctagtt atgatttttc taaattaaat ttcttcaacg
    18481 tccactaggt gagaaacagt attgatcatg cctttaattg cttcattaga aggcttaacc
    18541 actgaactgt taatctttgt aagacctaac gctttaactg tatcgcgttg gttttgagga
    18601 cgtccgataa tactgcgttt taaagtaact tttaattcag ccattattgt tgtcctcctt
    18661 atccgattaa ttcttcaact gatttgccgc gaagttctgc aacttcttct gcacgtttca
    18721 attgtttcaa accttcaact gttgcgcgaa caacgttgat tggtgtattt gagcctaaag
    18781 atttagaagt gatatctgct accccagcta attctaatac ggcacgaact ggtccaccag
    18841 cagcaacccc agaaccttct acagcaggtt tcattaggat tctaccacca ctaaaagcac
    18901 cgattacttc gtgtgggatt gttgaaccaa ccataggtac ttcaactaag tttttcttcg
    18961 catcttcaat tgctttacgg attgcttcag gaacttcttg agctttacct gtaccaaacc
    19021 ctacgtgtcc gtttttgtca ccgacaacaa ctaaagcagc aaaacgtaga cgacgtccac
    19081 ctttaacaac ttttgttaca cggttaattg caacaacgcg gtcttctaat tccaaatgtt
    19141 ttggatcgat ataaaccatg aatggtattc ctccttcttc taaaattcta gtccattttc
    19201 gcgagcagct tcagctaaag cttgcacacg gccatggtaa aggtatccac cacggtcaaa
    19261 gactactact ttaatgcctt tttcagctgc acgttcagca actagtttac cgacagcttg
    19321 tgctgcttct gtttttgttc cacctgaaat ttctttatcc aaggtagagg cacttgctag
    19381 cgttacaccc gctacgtcat caataatttg agcgtagatg tttttgttag aacgaaaaac
    19441 gttcaagcgt gggcgctcag cagtaccaga gatattgtta cgtacacgac gatgtctctt
    19501 ttgacgtgtt ttgttcttat ctggttttgt aatcacaatt gtcacctctt tatttttatt
    19561 gtactactat ttcaagcagt aaatacgttt cgaaaaatag aagctgcggc acaagtaaaa
    19621 atgtattttt ttgaaggtaa acttcagcca aatgcctttt tcctgctgct ggcttctatg
    19681 caatttttct
//
LOCUS       DACEZN010000039        19682 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEZN010000039 DACEZN010000000
VERSION     DACEZN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.039"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..694)
                     /locus_tag="IXH60_001076"
     CDS             complement(<1..694)
                     /locus_tag="IXH60_001076"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HAQ1990029.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTP"
     gene            complement(719..1726)
                     /locus_tag="IXH60_001077"
     CDS             complement(719..1726)
                     /locus_tag="IXH60_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1990030.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(1898..2257)
                     /locus_tag="IXH60_001078"
     CDS             complement(1898..2257)
                     /locus_tag="IXH60_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990031.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2229..2600)
                     /locus_tag="IXH60_001079"
     CDS             complement(2229..2600)
                     /locus_tag="IXH60_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1990032.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(2617..2919)
                     /locus_tag="IXH60_001080"
     CDS             complement(2617..2919)
                     /locus_tag="IXH60_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990033.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(2936..3406)
                     /locus_tag="IXH60_001081"
     CDS             complement(2936..3406)
                     /locus_tag="IXH60_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1990034.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(3381..3659)
                     /locus_tag="IXH60_001082"
     CDS             complement(3381..3659)
                     /locus_tag="IXH60_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1990035.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(3656..4705)
                     /locus_tag="IXH60_001083"
     CDS             complement(3656..4705)
                     /locus_tag="IXH60_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1990036.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(4662..5591)
                     /gene="cpaF"
                     /locus_tag="IXH60_001084"
     CDS             complement(4662..5591)
                     /gene="cpaF"
                     /locus_tag="IXH60_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1990037.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            5723..7477
                     /gene="ade"
                     /locus_tag="IXH60_001085"
     CDS             5723..7477
                     /gene="ade"
                     /locus_tag="IXH60_001085"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1990038.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(7575..8237)
                     /gene="trmB"
                     /locus_tag="IXH60_001086"
     CDS             complement(7575..8237)
                     /gene="trmB"
                     /locus_tag="IXH60_001086"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1990039.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8307..9086)
                     /locus_tag="IXH60_001087"
     CDS             complement(8307..9086)
                     /locus_tag="IXH60_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1990040.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9293..10504)
                     /locus_tag="IXH60_001088"
     CDS             complement(9293..10504)
                     /locus_tag="IXH60_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990041.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(10497..11234)
                     /locus_tag="IXH60_001089"
     CDS             complement(10497..11234)
                     /locus_tag="IXH60_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990042.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11372..11806
                     /locus_tag="IXH60_001090"
     CDS             11372..11806
                     /locus_tag="IXH60_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1990043.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            11799..12143
                     /locus_tag="IXH60_001091"
     CDS             11799..12143
                     /locus_tag="IXH60_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1990044.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            12323..13333
                     /locus_tag="IXH60_001092"
     CDS             12323..13333
                     /locus_tag="IXH60_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1990045.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(13402..14346)
                     /locus_tag="IXH60_001093"
     CDS             complement(13402..14346)
                     /locus_tag="IXH60_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1990046.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(14330..17050)
                     /locus_tag="IXH60_001094"
     CDS             complement(14330..17050)
                     /locus_tag="IXH60_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1990047.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(17047..18267)
                     /locus_tag="IXH60_001095"
     CDS             complement(17047..18267)
                     /locus_tag="IXH60_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1990048.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(18548..19393)
                     /locus_tag="IXH60_001096"
     CDS             complement(18548..19393)
                     /locus_tag="IXH60_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1990049.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         6025 a         3930 c         3243 g         6484 t
ORIGIN      
        1 atggtgtgaa tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata
       61 caccatttcc taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt
      121 ttgccgcacg ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt
      181 tgaaatcttc ataataagac accggtaagc tatataaata attttgcttt ggcattgttg
      241 agtggaaaga tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg
      301 ctttgatccc cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa
      361 tgcggtccat tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa
      421 caacacggtg ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca
      481 actgatctaa cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga
      541 atttttgatt atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga
      601 cgatcccttt cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg
      661 aactcccagc attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt
      721 acagagcaga tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt
      781 cttttaatga agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt
      841 ttttctgtaa gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca
      901 attgaatcat tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt
      961 gatccgtttc taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat
     1021 atttcattga ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg
     1081 ttaaaaactc ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg
     1141 caaaatcgac aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat
     1201 ttgcttgtgt caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca
     1261 caccaattcc ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca
     1321 agttgcccat tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata
     1381 gttgttcaat aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg
     1441 cctgcaaatg ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt
     1501 cttctggctc gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg
     1561 tctcttttgt cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg
     1621 cattttcgac ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag
     1681 cttccaggtt ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt
     1741 ctagatatat tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt
     1801 caatcgtttt cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg
     1861 ttataacaga aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat
     1921 cattttttac ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata
     1981 gggactgttg tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg
     2041 aactttcggc aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat
     2101 aataattttt gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca
     2161 tcaataaaaa cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat
     2221 aacattttct actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag
     2281 tgtacataaa aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt
     2341 aacataggtt gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg
     2401 atgatatact gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg
     2461 tgactgctct tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct
     2521 ttacgctgca aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc
     2581 aatagactgc tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt
     2641 acttgttttt tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac
     2701 tccattatta tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct
     2761 tttgtgatac atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc
     2821 ctccccactt ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac
     2881 acagacagaa gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt
     2941 caattctttt tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt
     3001 tttcttccca ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa
     3061 aagtaataga agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat
     3121 ttattttatc tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa
     3181 agcctgtttg gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa
     3241 attggccgaa aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg
     3301 gaaaagaaag gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc
     3361 ctgatgctaa aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata
     3421 tagccttcat tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag
     3481 agctcttttt gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga
     3541 ttcctataac gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag
     3601 acgattagca tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg
     3661 gcaaaacctc ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa
     3721 tcaaagccgc tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac
     3781 gtttgaaaag ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc
     3841 cctttgcctc tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct
     3901 ggaactgctc agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat
     3961 gctgaatcag actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga
     4021 aaccttcatg aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat
     4081 atatcggtcc aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact
     4141 tcatcagacg ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa
     4201 cataccaatg aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg
     4261 aaccaataag ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg
     4321 tgagcaatag catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa
     4381 gccgaaactg tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta
     4441 actcgatctg taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg
     4501 cttgcccttc ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga
     4561 tcgtgagtaa taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca
     4621 tggtttgtgc aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc
     4681 caaatatttt cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc
     4741 tccataaatt gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct
     4801 tgataaatga caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg
     4861 attctgacca atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct
     4921 gttagtgctg ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt
     4981 agcaagtctg gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt
     5041 ttcggattga tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata
     5101 gtaatcactt gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat
     5161 ccgactggtc cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca
     5221 aaatctcgag aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc
     5281 actaaactct ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt
     5341 ttttctaaag aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc
     5401 tgtcccaaat acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc
     5461 cagaatgttc gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat
     5521 atatatacgt cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt
     5581 tttatttcca ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca
     5641 aataaaatga atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa
     5701 atttatcttg ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag
     5761 taaaaaaaag cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa
     5821 agaaatcatg gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata
     5881 cgaaggaaaa agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg
     5941 ccatgtgcat atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct
     6001 tcatggagtt actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac
     6061 aggtcttgag tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct
     6121 accttcttgt gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa
     6181 attagcccct tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca
     6241 agcagtagca aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa
     6301 gaaaaaaatt gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc
     6361 agcaggtatt cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga
     6421 tcttggtatg tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact
     6481 accggcgatc acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat
     6541 caatgattta gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg
     6601 aatcgatcca ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt
     6661 gaaaacacta ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct
     6721 taccaagctg ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca
     6781 actccaagac catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact
     6841 aaacgctcaa ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca
     6901 tgtgatcgaa attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg
     6961 acaaggcgaa cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga
     7021 gcgtcaccat caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca
     7081 aggagcgata gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa
     7141 cgacgaggat atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt
     7201 tgtcaacaaa cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca
     7261 ggaacctttt tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact
     7321 aggagcagct aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat
     7381 acccgaattg aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc
     7441 accttgtgga aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac
     7501 attacatctg taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa
     7561 gcagaaagtt agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact
     7621 cgataaatac gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct
     7681 tcaaattgac tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa
     7741 aaacttgcca gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca
     7801 tttggacgaa gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt
     7861 tcatgacgtt tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg
     7921 tcagcgaaat actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc
     7981 ggttgttcga tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca
     8041 atataattga tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata
     8101 ccaatctcaa tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac
     8161 aatgttggat cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga
     8221 cgatttcgga cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca
     8281 aatctccgca gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt
     8341 tcttcgttca tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa
     8401 ttcatggccg cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa
     8461 ctgatcagcc actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata
     8521 tccaccgcag ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta
     8581 catactaacc aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct
     8641 attgacggga tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg
     8701 agttcttccg gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga
     8761 ccaccgatac gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca
     8821 tcaatcacgt cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac
     8881 tcttgtgctg tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta
     8941 attccttccc ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg
     9001 tcctcatcac gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc
     9061 cactcttggt ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg
     9121 cagtacttat tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc
     9181 atttaattgc cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt
     9241 actgtcaacg ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg
     9301 acatcttctt caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat
     9361 aagccgctag cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa
     9421 aaataaccga cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct
     9481 gattcttatg gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag
     9541 gtaacagttg gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa
     9601 agtcgactac tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac
     9661 taaaatcaga acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt
     9721 ctcttttaat atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa
     9781 tttcaggaac atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt
     9841 ctacatgcac cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt
     9901 aatatagact tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg
     9961 atagaaacaa aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt
    10021 gttgatctag tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc
    10081 ataatatgat cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta
    10141 atggcatggc caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc
    10201 gaaaagctcg ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat
    10261 ctgctgcctc tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag
    10321 agattatgag ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat
    10381 ataatcccac accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat
    10441 aacgcatata tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac
    10501 tcatgtgcct gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc
    10561 tgaaattctt ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag
    10621 accacaaaac gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata
    10681 ccggctcctt cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga
    10741 tccaagccta aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat
    10801 aagatcatca ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga
    10861 ttagataacc gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt
    10921 atatcgtagg ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa
    10981 gaaggagtct ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag
    11041 gatagatgat tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg
    11101 atggtcgtac ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt
    11161 acatcgaaac tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg
    11221 ataattaaac tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa
    11281 atgacggtat tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa
    11341 gtatttattc tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat
    11401 caatcaagaa atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga
    11461 catctctcaa gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat
    11521 ttttgaatat gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca
    11581 agcattggaa aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga
    11641 actagcctat caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga
    11701 ggatgatttt tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa
    11761 agctattcaa gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag
    11821 gactgttatt tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg
    11881 ggaatgaaac aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg
    11941 atttaaataa cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg
    12001 aatctgtgat tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc
    12061 aagccgaacc acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc
    12121 cagagtatcc tgaacaaata taataatgat tattttctca acagatttaa ctatataagt
    12181 gagaaaagtt tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt ttgaaactac
    12241 cacgacaacg tttttaaagg aaatgggata tggtatagtt aatatataaa aataataact
    12301 aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc
    12361 gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat
    12421 gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa
    12481 ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa
    12541 agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac
    12601 ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag aatatattcg
    12661 taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt
    12721 gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag
    12781 tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt
    12841 agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga
    12901 ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg
    12961 tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt
    13021 tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact
    13081 aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg
    13141 tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag
    13201 tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa
    13261 gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga
    13321 ttcatcaaaa taaaagaaaa caaaaaaagc gaagacttat gtctcgcttt ttttgttttg
    13381 aatgaaaaaa ataaattttt gctataaatc ttttggtaca tagaaacttc ggttgtccat
    13441 accgaaaatc cgatcggtgt attttcctgg ttctgtttga cgaatcgaag tcaacatcat
    13501 ctgcatcgaa gcatcgatat tgtcgatttg atgtaatatt tctgcttcca ttattcttgg
    13561 acgtacaggt gagccatatt ctaataagcc atggtgtgct aaaaccatat gacgtaatac
    13621 gagaacatct tcgtcagctt cgctgatttt caacgcaaga caggctttag ttatttcttc
    13681 gtcaatcaat accagatgac cgatcaaatt tcctgcaagc gtatattctg tcgacatagg
    13741 accggaaagt tcaatcacct ttcctaaatc atgcaaaatg ataccagcat acaacaatgc
    13801 tcgattcagc tcaggatatt catcggcgat tgatttccct aagtgaagca ttgtcaaagt
    13861 atgataagct aatccactgg caaatgcatg atggtttctt tttgctgccg gaaactcaaa
    13921 aaattgtttt tgatatttat taagtagata gcgaacaata cgattccaat gcgcattggt
    13981 gatctcaaac aacaattgat tgatttcttc ttccatttct tctttcttca aaggggctct
    14041 ttccatgtat aaactaggct ctcctggctc atctaccctt gttaagcgca tatgaagaat
    14101 ctttacttga gggtttcctt gataaacctc tcttttaccg tttagaaaaa cgactgtgcc
    14161 ggctgtaaat ttattgattt cctcctccga agcatcccag tatttaccat cgatagtacc
    14221 tgaagtatct tggaaagtaa aagcaataaa tttttttccg tttttagcga ttcttacatc
    14281 tgcacttttg atcaggacaa attgttcaaa ctgctcatcg attactaaat cacgaatttt
    14341 tttcattatt tgctccttct aattgtttta cgatttggtt catctcatgg taatagctta
    14401 ccatttcttt atcagaagaa aaacaaatta tttgatactt ttctccaaat ttagctaaaa
    14461 gttcagctaa ttgatacttt cgctgatgat cataatgaag ccagccatca tcaatgatga
    14521 tcgggcatat cgaacggtta gcttctaatg agaggtaagc aaatctcatc gccataatca
    14581 gctgatcttt cgtacctgta gataactcat agatgggaaa ttctgtatat ccgtctgtta
    14641 aagtcagtgt atcatttttc agtaatatct tctgataatt tccttttgtt aaaataccta
    14701 aataatcacc tgctgctctt aacaactggg gtaattgtct ttcactcatc tctgttgaaa
    14761 gatcacctaa gaatgtcccg gcgatttcaa gtgctcccca ttcctccagt aattcctgta
    14821 attttgcttt ttgccggcta gcttgttgat aaagttcgtc caatgtgccg tctctttgca
    14881 actcttctac ttgaagttgg agtcgttgcc gattttcact tgcttctttt tcttcttgct
    14941 ggagttggga ttgcttttct ttcagttggc gcagtcttac agataatttc tcttttgtca
    15001 gctcttcagg aaatagtgga gtaagcattt ccgataattc attgtatctt ttcatctgct
    15061 gttcggcttg tttggctttt tgtaaaaaga caggtacttc cgaaggataa gataagcctg
    15121 cttgagtcaa ttgttcgctg tattcttgta tccatacttt ttgtttcttt ttcaattgat
    15181 tgatttcttg cttcaataaa gtattttgct gatagcttct ggcaaatttc atctgttcca
    15241 tctcttgtat aaaacgggaa agcaatacca ttttttctga aattgtctta tcttgtatcg
    15301 gtaaccattc tttaacaaac tcaaattttt ggtcgatttg tcgtagtttt tcttgtattt
    15361 cctgttctct tttatgtttc cgctcaaata cgccatataa tttctgataa tctatgactt
    15421 cctgtccata ttctttcatg agtttgagcg tatccatctt tcctaagtgc cgatgatccg
    15481 caaaacgata tacttcttgc tgaagaatct gtttttcttc tccagttctc ttttgttttt
    15541 cttctgcttc agctaattga gctaaggcta gatcaagttg tcctagtttt tcttgccaca
    15601 ttccttttat ctcagagaaa tcatttttag ttttaatttt tggttgcctt ttgtagaaga
    15661 aatagccaat catgcctgca ccaattagta aaaggatcac tttcaagggc attggtacaa
    15721 atatacctgc aatgattatt gctccgccag ccatcagcat atacggttta gatgaagaga
    15781 ctggtgctat ctctgactga ttttgtaata atgctggata ggtctcttcc aagtcattca
    15841 cttctgcttc aatcatctct tgctgttctt tagctatttg gagacgcaca gaaatctggc
    15901 tttctagatc agcaatcctt ctttcttcag ccagaagatg cttccattct tcttcatata
    15961 aaaattcttc cggtaacgga tttttctgcc aatgccacct ttgttccaaa atcttcaatt
    16021 ctgaatttgt ttctctagtc tcaatttgta atctttgcca ttcttcgttt aattgttccg
    16081 ctgtaaattg aagttgctgg agatctttta gttcctgctc ctgctctaaa taaaaataat
    16141 atctggctga ctgctgatcc atcccgctat gcttttccag tgttgcttct aattcttcca
    16201 aacgctcatt tgtttgctga taccgtttgg agaaagactc cagatctaaa agaagttgct
    16261 catcaggaag acctgtagtg ctgttttttt tgagagattg ccattcttca tataaagaaa
    16321 aattaagttc ttgctgtctg gctaatgaaa attgtttgtt cgcctctttt aattgtgcca
    16381 ataactgctg ctgttgcgac tcgtattcct gttcttgttg gatcaacttg ccaaaataag
    16441 cttcctgttc ttgtttttga tgaatttttt cttgcagctc tttccattca tttaattttt
    16501 gattgattag taatttttgg ccttttttct tgaataattg ttgtgctttc tgataatatt
    16561 cttgccgctt ttggaatact tgagaactgc ccgtgatgcc taaagaaatc agcgcatcat
    16621 gtagctcttt ttctctcaaa tgatctagtt ccatcaactg ctcttgctgg aaagtaaata
    16681 cctgctggaa taactctttc gtcaaaggtg cgatcagttt ttctagtaac tcatcacttc
    16741 ccacttgatc ccccagccac accttcgatt ttccgcgatt ttgctgcttg tagcgctcga
    16801 cagcaaattc accatataca ggatgtttaa gccatatttt gccgccatag gctgagccgt
    16861 cttttggtgt ataatccttt tttttacggc cttttgaagg gaaaccaaat aatattgctt
    16921 gaataaattg ataaatcgtt gattttcctg cttcgtttcc cccatagatc agctgattgc
    16981 ctgaataaaa gtcaaaactt ttttgacgcc attttccaaa agcagcaatc tcgatcctta
    17041 aaatcttcat tcctgatcct cctcaaataa aaaatcttga ttaagcagct gcttggcttt
    17101 ttcaagtgtt tcttcttgat attccggcag ttcgctcaat attctagccg cttcttgatg
    17161 actatatact tcatttaaaa tttgttggaa atcttgaggt gtctgatagt attgaaataa
    17221 ctgttccatc aatttagcag aagcagcaag tggggtcttc gtataaaaag tattctctat
    17281 gagagaaata cgccaaataa agaaatcatt tatcctatct gagaattcct ctaacaaata
    17341 gtctaatagc tctcctgatt gtatccgttc caaaacatct gtattgagat ggtcataatc
    17401 ttttaatttt atctcaatta aactaaatcc atcctctact tttgaaagct gctgacgaat
    17461 gtgaacaatg acctcttttg tcgttcttgc tttcctcaat gatatttcct ttgtcttcca
    17521 atagacctct gctactttaa tcggatgtat ttggcaactt cctttactta attcaactag
    17581 tagaatagat gtagaagctt gttccttttt agtatgacct tgaggcgcac ctggataaac
    17641 aattgtttgc ttttcattta agaccgttgg cacatggata tgccctaaag cccaataatc
    17701 gtatcccttt tcttgcatct ttgagggttg gaaaggagca tagtttcctt tttgagagac
    17761 gccaggttcc ccgtggtaca acccaatatg gtaatctgtg agttctctag aaggaaattc
    17821 catcactttg tctttttgaa tccacggttg acgataacta aatccactga gagagacagt
    17881 ttctccactt tttatcgtta ttttttttgt ctctaccgtt tcggatgtaa ataaatggac
    17941 atttttagga aaggagaacc aataccgctc tttttgatag aaatcatgat ttccgaatat
    18001 tataaataca ggtatatggt tcttttctaa ccgtttcatc tgttctgaaa aatgtttctg
    18061 tatttttaat gaaggacgat tttgatgaaa attatctcct gcaagtaaaa caaaatcaac
    18121 gttatttatt attgcctgtt caatgatatt tgataaaacg gtcagattcg tcttcaatag
    18181 tttttcctgt acagttttat caagagttgt cagtccttca aatgaccgat ccatatgtaa
    18241 atctgccgca tggataaatt tcaccattaa aaatttcctc ctttttttct tttatcatac
    18301 cattttcaat gttttttttc atcaataacc gaaaatttgt tcgtattatg caaagaaatt
    18361 acgataaatg gccgacttca aaaaataagt ttattcgaaa aaaagattgt gacatgattt
    18421 ttgtcacaat cctaaatatc tatgttatat tcgcttcgtt tgacactgaa gaagtactat
    18481 ttgtactgga atttatgctc catttttccg tatttagaat tctatgttct atactacctt
    18541 aattagttta tatggtaaga attctttcaa atgcttgaga tactccgtct ttatcatttg
    18601 aaacagtgac ataatcagct tttttcatga tttcttgatg actatttccc attgcaaaac
    18661 taatatctgc actttcaaac atagataagt catttaaact atctccgatc gcgattctca
    18721 aaggtttctt taaattgtag attgattcca aacgatcaaa caccacacct ttagatacat
    18781 tttgaggtac gatttcaata tttacaggtg ccgattcagt aacagacaca tttagcatct
    18841 ttttcaaatg gttaaatata ttagctttca attgacgatt aggaataaaa ataaaaaatt
    18901 tgtaaatgct gatagcatcc ttttctataa tttcctcaat attattataa gggattgaac
    18961 aaatagattt ttcatactca agctcatagt ttagctcttt gatagaaaat tcactcccta
    19021 cctcgtgaaa aatttttttc attttatttt tataattctg agattcataa cttccgagat
    19081 ttgtatatat tttggttgga aatttcgata ggacagaatc aatttttttt acatcatttt
    19141 tatcgaagga agactcaaat atcttttttt ctccatcaaa tccaacagaa ccatttaacc
    19201 ctactgctgg tatccttaaa ccagattttt tgagaatatt attaatgtct tctacttctc
    19261 ttccagaaca aatgaatgga aaacagtctt ccggtaaatg tttcattgtt cttgcatttt
    19321 tttgactaat gctctggtca gaattcaaaa gagtaccgtc taaatctata ccaattagtt
    19381 tcatttcatc catgtctcct atacgttgat tataaattca ctgcttattg taagtctaaa
    19441 tttctcacaa aacatatact aattatacct aatttaagta agtacaaaca tcattttaag
    19501 aataggaaag gacatttata tactattctc tgtatttttg atttataaat aaatatgaat
    19561 tgaatccaaa tcagctatat tactatttta taataaccaa ctataaaaaa cgaagttagg
    19621 tgaaaaacta acttcgatat tcataaaaga ctctattagt tcaacttagc aaaagattta
    19681 aa
//
LOCUS       DACEZN010000040        19573 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEZN010000040 DACEZN010000000
VERSION     DACEZN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.040"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..782)
                     /locus_tag="IXH60_001097"
     CDS             complement(<1..782)
                     /locus_tag="IXH60_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1990050.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWH"
     gene            980..2497
                     /locus_tag="IXH60_001098"
     CDS             980..2497
                     /locus_tag="IXH60_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1990051.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            2487..3758
                     /locus_tag="IXH60_001099"
     CDS             2487..3758
                     /locus_tag="IXH60_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1990052.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3933..4829
                     /locus_tag="IXH60_001100"
     CDS             3933..4829
                     /locus_tag="IXH60_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1990053.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            4843..5706
                     /locus_tag="IXH60_001101"
     CDS             4843..5706
                     /locus_tag="IXH60_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1990054.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            5712..6275
                     /locus_tag="IXH60_001102"
     CDS             5712..6275
                     /locus_tag="IXH60_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1990055.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            6285..7808
                     /locus_tag="IXH60_001103"
     CDS             6285..7808
                     /locus_tag="IXH60_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1990056.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            7820..8779
                     /locus_tag="IXH60_001104"
     CDS             7820..8779
                     /locus_tag="IXH60_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1990057.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            8865..9944
                     /locus_tag="IXH60_001105"
     CDS             8865..9944
                     /locus_tag="IXH60_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1990058.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(10196..11596)
                     /locus_tag="IXH60_001106"
     CDS             complement(10196..11596)
                     /locus_tag="IXH60_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1990059.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            11814..13475
                     /locus_tag="IXH60_001107"
     CDS             11814..13475
                     /locus_tag="IXH60_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1990060.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            13432..14130
                     /locus_tag="IXH60_001108"
     CDS             13432..14130
                     /locus_tag="IXH60_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1990061.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            14146..15570
                     /gene="araA"
                     /locus_tag="IXH60_001109"
     CDS             14146..15570
                     /gene="araA"
                     /locus_tag="IXH60_001109"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1990062.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            15877..17037
                     /locus_tag="IXH60_001110"
     CDS             15877..17037
                     /locus_tag="IXH60_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1990063.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            17155..19014
                     /locus_tag="IXH60_001111"
     CDS             17155..19014
                     /locus_tag="IXH60_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1990064.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            19124..19558
                     /locus_tag="IXH60_001112"
     CDS             19124..19558
                     /locus_tag="IXH60_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990065.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
BASE COUNT         6431 a         3308 c         4163 g         5671 t
ORIGIN      
        1 aaatgccaag tgtcttgctc tttagggatg tcgttgatcg tgatgtttga caagggttct
       61 ccattcatat tcgtcaagtg tttggtaatt cggatcgtct tcagcaagcc gtattgatta
      121 agattatctg gataccaatc aggcgtatat ttaccgcgga tatcagcaaa atctccaggg
      181 ctcgtccatg ttcctagctc cacaccattt aggctgaaag tgatatctga aggccaattg
      241 tcatttgaaa aaggaaattc agaagaaatt tccacagaaa cttccaacat ctccaatgta
      301 tcctctgtat ttaaaaaatt aggtgcctga tactcgacaa atccgctggt aaaccagaga
      361 atgcgggcat ccatccttcg aggatccata aaatagcgtg gttcatccac tttcccgata
      421 aaatcatgga tcgtagctaa accgcatgtt ggttcaattg cataatttgt atagtgacca
      481 ataggtatcg atgtttcttt cgtatcaaaa gcattgaata ttttttctgg aaaactgata
      541 tcgattttat cgacacgaag gctcgaaatt ttttgttgtc ctactttttc tgttttgatg
      601 atgtttgctt cctctagctt tttcacgtgc atcgtcgtga tagcactgct cactccaagt
      661 tcttttgcca aatctttgac attcatcttt tttttggaaa gtagctggat gatcttgatc
      721 cttgttttac tagctaatgc ttcatatacc ggtaaggatt cttcgtttat ttgtagttgc
      781 atatatatca cctcttaaaa ggtttttctt atatattaac atatttgtta ataaaagtta
      841 tattaaatta tttgttttaa tcattattaa tattttgatt aattaaaatc gataaaaaat
      901 aagtaagttt aacatttata ttattgacaa cgctttcaac gaggtgtatt attcatgtat
      961 aaattaaagg aggtcagtta tggaagccac aatgttgatt agtagtgtca agcgtgtagg
     1021 caaagttgat ccgcggttgt acagttcatt tattgaacat atgggacgag cagtatatga
     1081 agggatttat cagccagatc atccatcagc taataaaagt ggatttcgcc aagacgtcat
     1141 tcacttggta aaagaattga acattcccat gattagatat cctggtggaa attttgtgtc
     1201 aggatttcgt tgggaagaca gtattgggcc catagcaaat agacctagaa ggttggattt
     1261 agcttggcgg acaattgaaa cgaatgaagt tgggattcat gaattttata actggtgtca
     1321 gcatgtgggg gccgaaatca atatggctgt caatctgggt actcgtggag tagatgcagc
     1381 tcgtaattta gtcgaatatt gtaattttcc taaaggaact tattggagtg atttaaggcg
     1441 ttcgaatggt caagagagcc cttttggaat taagacatgg tgtttaggga atgaaatgga
     1501 cggtccttgg cagattggac ataaaacagc ggaagaatat ggacggttag cagaggaaac
     1561 ggctaaggcg atgaaactcg tagatgattc aattgaggtg gttttatgcg gaagttcgaa
     1621 tcggcaaatg cctacatttg gtgattggga gttaacagtt cttgatcaag cgtacgatca
     1681 aatagattat ttgtctctgc atcaatatta tggcaaccaa aaaaatgatc tttctcatta
     1741 tcttgcacgt tccttagaca tggatcggtt catttctgaa gtgacagcga tgtgcgatac
     1801 agtcaaggcg aagaaacata gtaagaagca gatcaacctg tcctttgatg aatggaatat
     1861 ttggtatcat tcagcggaac aagatgagca agcagcgcct tggcagattg ctcctccttt
     1921 actagaagat aactataatt ttgaagatgc attactatta ggctgtctat taatcacttt
     1981 attgaaacat gcagatagag taaagattgc ttgtcttgct caattggtaa atgttattgc
     2041 accaatcagg acagaaacaa acggagaagc ttggagacag acgatctatt atccatttat
     2101 gcaagctgct gtgtatggac aaggagaggt gttaagccca caaattcgtt cagcaaacta
     2161 tgcgacggaa gattttgaag aagtgccgta tttagaaagt atagccgtaa taaatgacaa
     2221 agaacttgtc atatttgcag ttaaccgtgg agaagaagaa atgaatctaa ctgttcactt
     2281 gtctgaatta tcattagagg gagtgattga tttttctgaa atgagtggat ttgatacaaa
     2341 agcaactaat cttcctaaaa gtgaacaagt aaaaccaagt cattcaacaa atgtaaaagc
     2401 tgaagatggg aaaatcaatt gtttattgaa accgctatct tggaatgtgg tcagatgtcg
     2461 tttatcagat gaaggaggaa caaaaaatga aaggtaaaaa atggttaatt ggctctttgt
     2521 tggtagctgc aacaggtatt ttaggagctt gcggtggcgg aagttcagca gaatctgatt
     2581 ctgataaaga agtgactttg actttttgga atgggtttac tgcatctgat ggtgaaatat
     2641 tgcaagaaat cgttaatgaa tttaataaaa caaatgataa aaacatcaaa attgaaatgg
     2701 atatcatgac ttgggccaat ttgaatgaaa aacttcctac agcgatttct tctaaaaaag
     2761 cacctgactt tattgcatta aattatccag attttgcaca gtatgtagaa aacggagcgg
     2821 tacaaccatt agacgatttc tgggaatatg atggagtaga taaatctgat tttaaagaaa
     2881 cggctattga attaggtgaa gtagaaggta aacagtattt tgtccccatg caggtacaag
     2941 gcatgtatat gtattggaat aatgatttgt ttaaacaagc tggattagat actgaacaac
     3001 cacctactac ttgggaagaa ttgacagaaa tggcaccaaa attaactgat agtagtaaaa
     3061 atgtatcagg ttttgtcttc aataaggatg gaactgcacc gttatataac tggatgttag
     3121 caaatggcgg taaattagtg aacgatgatt atactaagtc tgaatttgcg tctcctgaga
     3181 accttaaaac gttaaaagcg atccaaaaaa tgatccatgt agacaagact ggtccagaat
     3241 cgatttctgg cgccgaaatg gacaatttaa tgaatgctgg tcaactagcc attgaaatca
     3301 atgggccatg gttaaataac ggattgaaag caaacgagat tgattataat gtgactacct
     3361 taccacaagg gagtgattca gacaaaaaaa cagctatttt agatggcgta ggatatgcca
     3421 ttccggcaag tacagataat aataaaaaag aagctgtcta tgagtttttg aaatattgga
     3481 atacaactga aattggtaaa aaatggagta tcgaaaatgg ttttcctgcc tacttggatt
     3541 cagtagcaaa agatgaagaa gtaaaaaatg atcctattgt atctgaatta tcgaaacaaa
     3601 tggactatgc agagccatat ttaccaggtt ttacccaaat ctctgcgatc aataacgata
     3661 tcatcaatcc actgattgaa aaattattag caggagaaga tcctgagaca ctcatgaacg
     3721 atgcagatga tgccatcaat caattactgc aaaaatagta cagaacttta tacaggatag
     3781 actgttggca agatacagat atctgaatga tcgacctagt atttgcagat aggtgttatt
     3841 gcaaaaatca cagcgattgt tcggatatct tattcagatt cgtttaaacc aagattgtat
     3901 attttatcaa aatgaaagat gaggtggact atatgaacgg attcaccaaa tattggaatc
     3961 gtcctgatcg tgctgcgtat ttgtttttaa ctccatcttt tttagtttta ttattattta
     4021 ctgtcatacc gcttttagga acatttgcta ttagttttac agatttgaat attttcttta
     4081 catcaagaaa ttttgtaagt tttgaaaatt tcacacgtat atttagtgat gaacgagcag
     4141 ttaattcgtt gattcattct ttatatttca ccgtactcga aacgccaaca caaattatcg
     4201 ttggtttatt agcagcagta gcattatcta aaaataccag attcaatcgt ttttgtcgtt
     4261 cgacatttta cattccagtt atttgttcat tgacgtcaat tgcgattatt ttttctatgt
     4321 tgcttgatcc gaatgtaggg gcgattcctt acttgttttc acaagtcggc ttgccgatcc
     4381 ctcaattttt tcgcgatcca acccttgcta tgccgacggt agccatcatg actgtttgga
     4441 agaattttgg tgttactatg acgattctct tgactgctat tcagggaatc tctccctctt
     4501 tgtatgaatc tgctcaaatg gatggtgcaa caaatcgaca gcagtttttt aacattacgc
     4561 tacctcagat cgttccctcc ttaggttttt gtattttaac gaatctgatt ggttcgatgc
     4621 aagtgtttga ccaagtatat gttgcaaccg ctggtggccc acaatttaag acagaaacag
     4681 ctgttcaata tatttacagt cgaggattta cagcacctta tgaattgggc tatgcatcag
     4741 cactgtcttc ggttttgttt atcattgttg caactatagc tatctccact aatgtgtaca
     4801 tgtcacggaa agaaaaacaa atgaaatagg agggaaacct gtatggcacc agaaagctcg
     4861 attacagtta ctgtgaatag tcgaaaagca gttagtggta aaaaaatcat tcgaacgatt
     4921 agtatgctag ttatattgtt ggttattgcg tttccttttt tgtggctgat tatttcttct
     4981 tttaaacacg aaaaagacat tatctcattt ccgcctagaa tatttgcaga tagctatacc
     5041 ctagataact atattaaggt ttggacaacg atccctttat tagattacat caaaaataca
     5101 gtgatttttg ctggagggac agtgatcact tctgtattct ttgattctct tgcggggtat
     5161 gcatttgctc gtatgcgttt caaaggaaaa tcagttttgt tctatttcgt tttgctgaca
     5221 atgatgatcc cattccaagt cttcatgatt cctctattta ttgaagtcaa tttgcttggc
     5281 atgcttgata cttatgcagg tttgatcatt cctcgtatga caactgcatt tggtatcttc
     5341 atgatgcgtt ccttttttat tactttacca gattctttgg aagaggcagc acgcattgat
     5401 ggattaagtg agtttaatat ttttttgaaa atcatgttgc cattgagcaa acctactctt
     5461 ttatcattag ggattttcac gctgatgaat agttggaatg acttgcttta tccattgata
     5521 ttgacaagta gttcaaaaat gagaacttta ccagcagggt tagctttatt tacagggcag
     5581 aacatttctt tttatggacc tgtaatggct ggaacggtta tttcaatgct gccattacta
     5641 gtggtttata tttttgcaca gaaatatttt gttcaaggaa cagctatgtc tggaatgaaa
     5701 gagtgaggaa gatgaaaata gttgagacta aattttatgg acagttgatt gtggtttctg
     5761 attacatctt attaggtatt ctctgggtaa tcgtgtgttt gcctgttatt actatcgtgc
     5821 ctgctacatg tgctgttttt tatgtaatgg cgcaatggaa aggtgaggga tcaggaaata
     5881 tcatttattt tttctttcaa ggaataaagc aacattttgg taagaacttg ttccttagtc
     5941 tgttaacgat cattatttat tttcttgtta atcaattatt agaagaacag acaatcgtac
     6001 tttcaattag tggatatgta gttttactta tttattccat gtttctttta tcctggatat
     6061 atgtgatttc tgaaaacaaa gatatcacat ttttacaaat ttttgaacaa agtgcatgga
     6121 aagtattttt tgattttttg agaaatattc tttgttgcgt attgttatta ggatttgtac
     6181 tgctcatatt tcttttccct ccttttattt ttatctttgc tggtggcgta tggaaacttg
     6241 tatctattat cttgactata ggaaatgaaa ggtagggaaa gctgttggac gaagaagtga
     6301 aagtaaaaat cagagaggat tttacggtag gagagatcag ttctcggctg tttggctcat
     6361 ttgttgagca tatgggaagt gtcgtctata cagggattta tgaaccgggc catgaaacag
     6421 ctgatggtga cgggtttaga caagatgtcc tctcttatgt aaaggaatta gaattaggtg
     6481 tcattcgcta tcctggagga aactttacat ctggttatga ttgggtggat actgtaggta
     6541 ggaaagaaca acgaccaaaa aaaattgatt tagcatggcg aggtatagaa ccaaattctt
     6601 ttggactcca cgaatttttt aaatggatca gtcatgtaca agcgaaaccg atattcacgg
     6661 tgaatttagg aacaaaagga attgatgaag caaaaaattt agtagaatat tgtaattttc
     6721 caaaaggaac cttctggagt gatttaagaa taaaaaatgg gcaaaaaaag ccttttggcg
     6781 ttaaattatg gtgtttaggg aatgagctag acggtccatg gcaagtgggg gctaagagtg
     6841 cttatgaata tggtaggtta gcaaatgaag cctcaaaagt catgaagtta gtggatgatt
     6901 caattgaaac aatcttagtt ggaagctcta cgccacgttt accatcgtac cctgaatggg
     6961 atcgtatcgc attagaagaa gcttatgaga atgtagatta tttagctttg cataactata
     7021 ttgataagta tgatgaagaa gacttgacta aacctcctaa aaatgagcgt gatgatacag
     7081 cgacatacct tgcaaaagct tatcgattcg atcgtcaaat tgaagaaatc gttgctactt
     7141 gtgattatgt aaaaggatat ttgagaagtg agaaaacaat gtatctagcg tttgatgaat
     7201 ggaatgtaca tgcccaaccg gaaaaaagac atagggattt tgaagtcggt agtcccatcg
     7261 attggtgtta ctttactatg gaagatacat tattatttgg ttcattaggg ctggctattt
     7321 tacgacacgc agatcgcgta aagattagtt gtcaatcatt gttagtaaac acaatacctt
     7381 taatactgac agatgctaaa ggacaagcat ggaggaatcc aacttattat gtgatgcaac
     7441 acttatcgaa atatgctaaa ggtcaagtat tgaaacaaca aatcatttgc cctgtttatg
     7501 attgtgaaaa gataaaaaat gttccttatg tggacagtgt gatcgtagat aacggcaatc
     7561 agctgactat tttcttgatc aatcgcacaa atgaacaaca aaagataacg attgaacatg
     7621 atttcaattt gctagatcaa ggagagcact acacattaac cagtagcgat ctgttggaca
     7681 aaaatacacg tgaagaacca gaaaaaataa aacctgaaat aaactatttt tcaatagaaa
     7741 aacatgacga attaaatgta gggatagaaa aatactcttg gaatgtaatt agaataaaga
     7801 aaatgtgagg aaatttgtta tgatgaagac actttataat aatcctatcg ttcggcaacg
     7861 ggcagatcct tgggtatata aacatacaga tggttattat tattttacag gatcggttcc
     7921 aggctaccaa gtgattgaat taagaagggc aaagtcactg aatgaattag aacatgctgc
     7981 aagcctgatt gtatggcagg ctcatgaaga aggaccgatg agtgaattga tctgggcacc
     8041 tgaagtccat tatatcaatg gaaaatggta tatttacttt gctgcaagca atcataaaac
     8101 tatacgtgat gaatctcatc atcaccgcat gttcgtactt gaaaatcagc atatagatcc
     8161 aatgaataca gattggatag aaaagggaca aataaaaacg aaaagggaaa ctttcagttt
     8221 agacggaaca actttctctt ctcaaggaaa gctatattat gtgtgggccc aacaagatcc
     8281 tgaaattact gggaattcta atctttatct atccgaaatg gataatcctt ggacgttaaa
     8341 ggggaagcag atcctccttt ctatacctga gtatgaatgg gaaaaacggg gtttttcagt
     8401 aaatgaaggt ccagcagtcc ttactcgcaa tgggaaaata ttcattacgt attctggtag
     8461 tgcaacagat gaaaactatg ccatgggcct gctgtgggct gatgagcaca agaatctttt
     8521 attaaaagaa tcatggcata aattatcgga gccagtattc gtttcttctg aaaaaaataa
     8581 gcaatttggt cctggtcaca actcgtttac ggtttcagaa gacgggtgct ttgatatttt
     8641 gatctatcat gctcgaccag agaagaacca aaaaggggat ccgttagaca atcctaatcg
     8701 gcacgcgaac gcgcaatgct ttacgtggaa tgagcaagga tttccagaat ttggtgaacc
     8761 tgttccgtat gccttatgag cacaagaaac cttgattttt gcttgaataa accttgcaga
     8821 ctcgttatga tatatgtacg tacaaataaa aaggggcaag gcaaatggca aaatatcaag
     8881 atattgcaga agagataaaa caaaagatcc ttcgtgagga atacgaagta aatacaacca
     8941 ttccctcaga attcaagctg caagaaatat atgacgtcag cagacatacg attcggcaag
     9001 caatcgctgt gttggtaaat gagggctatc tgcgtaaaga aaaagggtcg ggcacatatg
     9061 tagatgacag ctataagaaa gaaaaaacac cattgaacag aacaaaaacg atcggtgtca
     9121 tcaccacata tctctctgat tatatttttc cgtcgattat ccgaggaatc gaaaatgaat
     9181 tacgagaatc tgggtattca ttgcttttag ccagtacgaa taacgatcac gaattggaga
     9241 aagagtgttt gaagaaaatg attgcttatg gggtagatgg tttgatcgtt gagcctacga
     9301 aaagtaatca gtataaccca aacttagcac tttatgttgc tttgagagaa caaggcattc
     9361 cgatggtgat gatcaatgct gcttatgaag agttaaatgc ctcttatatt tgtttagatg
     9421 atgtgaaagt cggttttatg gcagcagaat atttgataaa aaaaggacat cagcgtctgt
     9481 tgttcatcac aaaaatcgac gatcttcaag gaaaatacag gatgaaaggt tttatcagcg
     9541 cgtgtgaaca gtataatgtg cgttttttga cggaagatat cattactttc accacggaaa
     9601 cacgagaaac aatcgctgaa aaaacagtac aacggttgaa agaagaacaa gctttagatg
     9661 gcatcatttg ctataacgat caaatagcta gtttaatgct agataagttg acagaaaatg
     9721 gctatcatat tccagatgac ctatcgatcg taggaacaga cgattcttct ttaagccatt
     9781 tagggaataa aaaattgaca acattgacgc atccaaaaga aaagttggga caagatgccg
     9841 cacggtggat cgtcacaacg attgaaaaag gacaggcaca gccaaatatt ctctatgaac
     9901 ctgtactagt tgaacgggaa acagtgaaag aaaacgaaaa ataagagacg ggtttttacc
     9961 gcctcttatt gctcctacgg ttactcgtcg caatgtttgt gagagctttt gtcatcgctc
    10021 aaggctgaac gtttctttca ccacctctcc actaaacggt gttctcctac caactggtca
    10081 caagtcacaa tattcatcaa acatgagaag ctgttttttg aaattgttcc ttgtgactgg
    10141 aaactaagcg gttccttctt atttcttgaa gctgaccact tctgtcacaa cctctttaag
    10201 aatgatggat agaaatacgt ttaggataag tttttttaga tgcactttgt tcgatttcct
    10261 ctaaagattt cccacgagtt tctggtacga tctcttgaat aaataaaacc cctaaaaagc
    10321 agatgatccc aaaaatgccg aaaacgattt cttgagacat agaagcagtc atcacaggaa
    10381 aaagcaagcc aactaggaaa gaaccaatcc aattaaaaga agaggctagg ccagatgctc
    10441 gcccgcggac agctagtggg aatatttcgc ctatgattac ccatgtcagt ggagcccatg
    10501 tgcaagaata aaatgcgaca taaatacata aaaacaagag aatcaacaac gaatttgtat
    10561 gagcatccag tacagttcct aaaacagctg gtaggatgaa tgaaagtccc atcacgctac
    10621 cgcctatttt caataaagtt cgccgattga acttgtctgc aataaccaaa aagagcaaag
    10681 aaccagcaac taaaatgatt ccttggatga tcggccacat caatgcatcg ctcgctgcat
    10741 gcccagtcgc tttttctacg atcagcggaa tatagtaaaa aatagcatta gctccttgaa
    10801 attgctggaa tactgctaca ccaataccgg caatgactaa ggaacgatat ttttctaaaa
    10861 acaaagtatg ccaagaagtt ccggttgtct tttcttgttt gacattcagc tggatttgtt
    10921 cgaattcctg ttcagcttct tttggtgtcc gaatatagtt caatactctt tttgcctctt
    10981 ccaagcggcc agattgcata agaaaacgcg gtgactctgg tagagctaaa acaccgaaaa
    11041 ataaaatcag cgcggggaca gcagcgagcc ctaacatcac gcgccaagcc atcgtttcag
    11101 gcaacacttt tagcaaataa tctgcaacat aggaaaggag catgccagaa gcaatcatca
    11161 cttgattgat ccctgacaat cttcccctta gtctagcagg agccatttca gacatataag
    11221 caggaactag ggcagatgca gcgccaacag ctaaacctag aagaatcctt gctgcaatca
    11281 gaaaataaga accattgtga ggggcagatg cagaaaggac agaaccagcc ataaaaataa
    11341 gtgcagaaag aagaatcatt tttcgtcgtc caagcttatc agaaagaaga ccagataaag
    11401 agccgccaag gatcgcacct aacatgaccg aagacgtgat ccatcctatc aaagaagctt
    11461 tacctgccaa accccaatca tgctgtaaaa aaggtaaggc gccggtcata acaccaatat
    11521 catatccaaa tagaatgccg ccaaatgcgc caaaaaaata aataaacgat gaggaaactt
    11581 ttttttcttt tttcataagt attctctcct tctagaattt cgagaaggcg gttgatcaga
    11641 ttttgtaagc aatttcattt tataacttat gtacgtacaa aacaagtgtt ttttattgta
    11701 ttcttgtaaa taaccgcttg acatgtacgt acaaaagtat acaataatca ttgtaagcgc
    11761 tactaaaaag acggtttgat caggtttgac gagaaggagg gagttaaatc attatgaagg
    11821 aagtaaatgc gcaaagtatg ttagatgtca tagatggtcg tacatcttta gggattgagt
    11881 ttggatcaac taggatcaaa gctgtattga ttgattcatc ttttactcct attgccgctg
    11941 gtagttatga gtgggaaaac aaactagaaa atggtatctg gacctattca ttagaagata
    12001 tttggaaagg actgcgcgtc agttaccgaa aaatggcagc agaagtattc gaaatctatg
    12061 gcgagaatct tacgacaatc gggtcgattg ggttcagtgc aatgatgcac ggttatctag
    12121 catttgatag caaggaacag ttactagtac catttcgtac ttggcgaaat acaatcactg
    12181 aagaagcaga acgaatatta acggaaaaat ttcaatacaa tattccgcag agatggagta
    12241 tcgctcattt gtatcaatct attttgaata aagaagaaca tgtgaaagaa attaattttt
    12301 tgacaacgct tgcaggttat gtccattggc agcttactgg tgagaaagta ctaggcgtag
    12361 gagatgcatc aggcatgttt cctatcgatc cttcaacgaa aacctaccat caagaaatga
    12421 aaaatatttt tggtcagtta atcgaaaaag cgcacgtagc gattgacttg gatcacattt
    12481 tgcctaaagt tctagtagca ggagaagaag ctggcaagtt aagcgaatca ggggcttatc
    12541 tgatcgaccc tacgggaaat ttgagaccag gaatcccggt ttgtccgcca gaaggggatg
    12601 caggaaccgg tatggtcgct acgaacagtg tagccaaacg gacagggaac atttcggctg
    12661 gtacttccgc cttcgctatg atcgtattag aaaaagaact agaaaatgtc catccggaaa
    12721 tcgacatggt cactacacct tcaggagaac tagtaggcat ggttcatacg aataactgct
    12781 cttcggatat caatgcttgg gtcagtattt ttgaagaatt taccaattct ttgggtatgg
    12841 agataagcag agataaatta ttcacggtat tattcaacaa agcgctagaa ggcgatacgg
    12901 attgcggggg attactttct tatggttatt tttccggtga aaacattaca ggagtaaatg
    12961 aaggaaggcc attatttgta cgtacaccag gtagccgttt tgatttagcg aatttcatgc
    13021 ggatccattt agccagtgct tttggtgcaa tgagaatcgg aatggatata ttgaaatctg
    13081 agaatgtcca aattgatcgt ttagtggggc atggggggat ttttaaaaca ccagaagttg
    13141 gacagcggat tctagcatct gctatggagg caccagttac agtgatggac acagccggag
    13201 aaggtggagc gtggggaatc gcgttattag cagcctatat gatggatcga catgggaagt
    13261 ctttagaaaa cttcttggca gaagacgtgt ttggacaaga ccaaggaatc accatcacac
    13321 cttcagcaga agaaatagca ggctatcaga tatttatgag acgttaccgt gaggggatcg
    13381 acattgaaaa aacagcgatt catcaattgc aagaaaaagg agtgatagaa aatgctggaa
    13441 caactgaaag aagaagtata ccaagcaaat cttgacttgc ctaaacacgg actcgtcaaa
    13501 tatacatggg gaaatgtgag cgcttttgat cctgaaacac gatatttcgt tatcaaacct
    13561 agcggagtca gttatgaaga gatgacagca gacgacatgg tcgttgtgga cttggacaat
    13621 catatcattg aaggtaaact caatccctct tcggatacgc ctactcatgc tgttttatat
    13681 cgttcatttc ctcaaatcgg aggaatcgtc catactcact cgacatgggc gacgatctgg
    13741 gcacaagcag gattggatgt tcctgcgatg gggacgacgc atgcagatac tttttatgga
    13801 tcggtacctt gtgctcgatt tttgacacaa caggaaatcg acagcgggta cgaatatgaa
    13861 acaggaaaag ttatcataga aacattcaaa gaacgcaaaa ttgatccctt agcaattcca
    13921 ggggtacttt tacacggaca cggtccattt acttggggga aagatgcgca aagtgcggta
    13981 atgaacgcag tcgtactaga tgaagtatgc aagatgaatt tatttaccag acagctcaat
    14041 tcattttcag aagagctgcc tcaacggatt ttagataaac attatttacg aaaacacgga
    14101 aaaaatgcgt attacggaca aaacaaataa aaggagtggc taattatgtt gaatattgga
    14161 gaaaaagaat tttggtttgt tgtaggatca cagcatttat atggggaaga agcactgcga
    14221 gaagtaaaga aacatgcaca agaaatggtc gatgaactga atgaaaatgg ccaattgcct
    14281 tatccaatcc gccttcaaga attagcagtt actgctgata cgatcacaaa aatcatgaaa
    14341 gaggtcaatt atcgagaaga agtagcaggt gtgatcactt ggatgcatac tttctcacca
    14401 gcaaaaatgt ggatccgcgg gacaaagctt ctacaaaaac cattgcttca tttagctacg
    14461 caatataacg aaagcattcc ttggaaaacg atcgatatgg atttcatgaa tttaaatcaa
    14521 tccgctcatg gtgaccgcga atatgggttc atcaatgctc gattaaataa acaaaataaa
    14581 atcgtcgttg gctactggaa acgacctgag atccaaaaag agattgctga ttggatggat
    14641 gttgcagtag cctataatga aagttttgga ataaaagtgg ctcgttttgg tgacaacatg
    14701 agaaacgtgg gtgtcacaga aggagacaaa gtggaagcac agattcaatt tggttggact
    14761 gttgattatt ttggtatcgg agatcttgta caagtaatcg atcgcgtatc agacgaagaa
    14821 gtcgatcaat tatttgaaga gtacaaggaa ttgtatacct tcgactatgg tgattatgaa
    14881 gaaaagacat gggaggaaca tgtaaaagta caagctcagc aggaaatcgg tattcgccgt
    14941 ttcttagaag aaggaggcta taacgctttt acgactaatt ttgaagacct gtatggaatg
    15001 aaacaactac ctggactagc tgtacaacgt ttgatggcag aaggatacgg ttttgctggg
    15061 gaaggcgact ggaaaactgc agcgattgat cggttgctga aaatcatggc tcgagggaaa
    15121 gatacaggat tcatggaaga ttatacttac gagctagcta gtggccaaga agcaatcttg
    15181 gaatcccata tgatggaagt agatccgacg cttgctgcga cgaaaccaag aatcgttgtt
    15241 tctcctttat caatgggaga tagagaagat ccggcgcgtc ttgtatttga tgggaaagca
    15301 ggagaagggg tagtcgtatc gatggcagat ttcggtacgc attataaact gctgatcaat
    15361 gaagtagaag cctttgaacc aacgactgaa gcacctaatt tacctgttgc acgtgtgtta
    15421 tggaaaacta aaccgaattt ccatgaaggt gtccattcat ggattcaggc aggtggcggt
    15481 catcataccg ttgtctcgct aaatctaaca acagatcaaa ttgaaacgtg ggcaaaatta
    15541 gttgagttgg aaacagtggt tattagataa aaagaggttg tgaaaaagcc acttagtttt
    15601 aagaaataag aagagattga cgaaatttgc ttttcaactt ttttgaaatt tagtcttatt
    15661 atcaatgtca gctaacggaa caccatttat caagatatct caccctcaat attttgctct
    15721 cgaaaataag gcttatggca gtaacttgtc atagccttat ttttatcact cagcctactc
    15781 tgctctaact gctctttttg cttctaggaa gtcaaatggt aaacagttgt ctaattgcag
    15841 aaatttcgag atacttgtta gtaaggaggg ggttttatgt atcacgttca tttgattgat
    15901 gctattccag aacaagagat agaaaagtta caaggggaat atttcaaaat aatagatgat
    15961 ttgccagccg aaggaatagt tattcgtagt acgaaagtga aagatgaatg gcttacacca
    16021 gatttgctag caatttcaag agctggtgtt ggggtgaaca cgatcaacgt tgaaaaagct
    16081 tctgaaaacg ggacgatcgt catgaataca ccaggtgtaa atgctaatgc agtaaaagag
    16141 cttgttttgt gctgtttgct cttatcttcc cgtccgataa tcgaagcaag tcgtatggta
    16201 caaacactaa caggtcctaa tattttagaa caggcagaaa acaaaagaag tgcttatgtg
    16261 ggacgagaat tgcaaggcaa aaccatcggt cttcttggtc ttggagcgat aggcacaaaa
    16321 gtagcgctaa gttgctatag cttagggatg gatgtcttgg gttattctat ccgcgatgcc
    16381 cagttggatt atgttcggca agcagattta gaaacagttc tttctacctc ggattacatc
    16441 gtagtcatgc tgccgttgac agaagataca aaaggattga tcgatcaggc gaatatagaa
    16501 aaaatgaaaa aagatgctgt acttatcaat gtaggaagaa gtgaaattgt tgataaatat
    16561 gctgttatgc aagcattgga aaaacaacat ctggctaaat acttgactga ttttccagaa
    16621 gaagagttct tagaaaatga ccgtatactt atgttgcctc atctaggggg atcgactcag
    16681 gaagctttgg cagacagcgg acgtttggca gttgaagcct taaaagatta tcttttgttc
    16741 ggtacggtta gagaagcggt caattatcca tccgcacgaa tgtttttcca agcgccgttt
    16801 cgattcacta tattttatca aaagaaaacc aatatattag cagagatatt ttctctgctg
    16861 aatgagaacg agctagcgat cgcagatata tcgcgtaacc ataagaatga gcatgtctac
    16921 atattgattg acatagacag tactgatttt gaacagctca atcaagtgaa acaacagcta
    16981 gaaaaaattt ctggagtacg aaaggtccgc ttattaaaga aaccagaaag gccataaaga
    17041 aagagaatat ttggatttca ttaattcttc tttccgttct ttactttcga atttcgtaca
    17101 actaaaataa gagaagcaac attcttttat tgatagaaat gaaggagcaa gcatttgaaa
    17161 agatatataa gcagactttc gaccaaacag aaaaagattt taaaaggaac agcagcaatc
    17221 atcagtatct tttttcttgt tgtttttagt aatttatttt tgcagtggtg tcaaaatgat
    17281 ctatccgtag accttgcgtt gaaatttgct ttttcttggc atacggaaaa attcttctta
    17341 gcctgtctcg tcttgctgat tattcttatt tttctgattg cactagcagg atctgtccct
    17401 ttaggttccc ttacgtatgt cgtagccatc ggtgtattag gttttgcaaa ttatatgaaa
    17461 atgagttatc ggcaagaacc gatttatcca gacgatttga agatgatcac tgaaatcgga
    17521 ttgctgaaag atatgaccgg cactatgctg tttacagtga tcttagctgc cgcaggaacc
    17581 gtgataggat tgttttgctg gtacatgttt cgaagcctga aaaaaggaag gcgattccaa
    17641 ttgatccgat taacgacact gcttgtggca attggactgc tagggtatat cagtaatttc
    17701 aacaatcccg ataatttgtt gcgaaaagca tataataaaa cggcactatg gataccttac
    17761 agccagaaaa tgaactacta taacactggt tttatcggtg ggtttttgta caatctgaag
    17821 gtagaaccaa tggatgaacc tgaaggatat tcgaaagaaa agatcaaaga aatcactgaa
    17881 aaataccaga agctagccga tgaaaaaaat aaaactgtag aggaagagtc gccgaacatc
    17941 gtgttcgtga tgagtgaaag tttctcagat cctagtcgat taaacggcgt agaagtatct
    18001 ggtgagccat tggcagatta ttatgaagta gctgatcaaa catatagtgg caatatgctt
    18061 tctcaaaact atggcggtgg aacagcaaat atcgaatttg aagcattgac tggtttttct
    18121 atggcgttat ttaatgcgca actcaccacc ccttacacga tgcttgtacc aaaaatggat
    18181 cagcttcctt ctatcgtttc agcgttaaac gcacaatcat accagacgac agcgatccat
    18241 ccatacaata cttcgatgta taaaagagag gatgtttatc agacattagg atttgaccag
    18301 ttcatcagcg agcgtacgat gacctatacg gatacgatag agaataatcc atatatttct
    18361 gatgagtcag cttacaaaga agtaatgaca cttctcaagg aagaaaaaac acctcagttc
    18421 attcatttag tcacgatgca gacacatatg ccctacgatg gaaagtatga taatctagac
    18481 tatcctgcaa aaacttcaga tggaagtggg acacttgact tagagaatta tctgcaagat
    18541 atttcctata gcagcacggc actgaagcag ttcacggaag aattgaagaa tctttctaga
    18601 aggactttag ttgttttttg gggggatcat cttccaggga tctattcaga tacgatccaa
    18661 gcaaaaaatg acaaacaaac attgcatgaa acacaatttc ttatgttcga cagtaaagga
    18721 gaattagaaa agcaaacgac tcacgatgca atcaccagtc cgttttattt tgcagcgaat
    18781 ctaatggaac agacgaatca gacaacaaat ggcttttatc agctgctttt atcattagaa
    18841 caggaacttc cggcgtttga acgagaactg tactatcaaa acggacagtg gtataaagaa
    18901 gcgcaattca atcgctcgca acaagagatt tacgacgaat atcaattgat ccaatatgac
    18961 attgtcgcag ggaaacaata tagtttagca gatggtttct ttgagcacga ataagaatgg
    19021 cgagctgtat agaaaccatt aaattttttc aagtacttgt catttgagga taaaaacaag
    19081 tagaataaat aataacaaca atggagaaag taggaaaacg aacatgttac cgaatattgg
    19141 ctattatgtg cagtccatcg atcaaatcgt caaggaaaca gaagcaatcg gagaaggaat
    19201 gcatccgaaa tatgaagtga tcagaaacgc aattgatgaa ggaaaaacaa gtgaactgga
    19261 caaatcgact cttgaagaga tcatcctgtt atttgaagaa ggaacaaaaa aatatcgggc
    19321 aatgcttaag aaagtcagta ctttgcgtcc accagcaaaa gcaatgggca ttcataaaaa
    19381 ttttgaaaaa gcttatcttt catatgtagc aggctgtgaa gaaatgatcc aaagtatcga
    19441 tcttgaatca ggggtagatg aagatctatt caatacatct gaagcaaaac aagaccaagc
    19501 gacagaccaa ttgacagctg ccatccaaaa aatgactaat ttgttgttga aaaaataaaa
    19561 gaaatctttg ata
//
LOCUS       DACEZN010000041        19308 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEZN010000041 DACEZN010000000
VERSION     DACEZN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.041"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            968..2218
                     /locus_tag="IXH60_001113"
     CDS             968..2218
                     /locus_tag="IXH60_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1990066.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2237..3247
                     /locus_tag="IXH60_001114"
     CDS             2237..3247
                     /locus_tag="IXH60_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ1990067.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3257..3910
                     /locus_tag="IXH60_001115"
     CDS             3257..3910
                     /locus_tag="IXH60_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1990068.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3929..4927
                     /locus_tag="IXH60_001116"
     CDS             3929..4927
                     /locus_tag="IXH60_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1990069.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            5003..5764
                     /locus_tag="IXH60_001117"
     CDS             5003..5764
                     /locus_tag="IXH60_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ1990070.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5782..6888
                     /locus_tag="IXH60_001118"
     CDS             5782..6888
                     /locus_tag="IXH60_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1990071.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6878..7111
                     /locus_tag="IXH60_001119"
     CDS             6878..7111
                     /locus_tag="IXH60_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990072.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7310..7765
                     /locus_tag="IXH60_001120"
     CDS             7310..7765
                     /locus_tag="IXH60_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ1990073.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7779..8552
                     /locus_tag="IXH60_001121"
     CDS             7779..8552
                     /locus_tag="IXH60_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1990074.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8539..9669
                     /locus_tag="IXH60_001122"
     CDS             8539..9669
                     /locus_tag="IXH60_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1990075.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9700..10935
                     /locus_tag="IXH60_001123"
     CDS             9700..10935
                     /locus_tag="IXH60_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990076.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10960..12069
                     /locus_tag="IXH60_001124"
     CDS             10960..12069
                     /locus_tag="IXH60_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990077.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            12111..13445
                     /gene="gmhB"
                     /locus_tag="IXH60_001125"
     CDS             12111..13445
                     /gene="gmhB"
                     /locus_tag="IXH60_001125"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ1990078.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13480..15117
                     /locus_tag="IXH60_001126"
     CDS             13480..15117
                     /locus_tag="IXH60_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1990079.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15219..15614
                     /locus_tag="IXH60_001127"
     CDS             15219..15614
                     /locus_tag="IXH60_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1990080.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15632..16363
                     /locus_tag="IXH60_001128"
     CDS             15632..16363
                     /locus_tag="IXH60_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990081.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16387..17838
                     /locus_tag="IXH60_001129"
     CDS             16387..17838
                     /locus_tag="IXH60_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1990082.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17864..18475
                     /locus_tag="IXH60_001130"
     CDS             17864..18475
                     /locus_tag="IXH60_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1990083.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18657..19172
                     /locus_tag="IXH60_001131"
     CDS             18657..19172
                     /locus_tag="IXH60_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAQ1990084.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
BASE COUNT         6223 a         2699 c         4217 g         6169 t
ORIGIN      
        1 ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca cgggtgatat
       61 atatgtatat ggtgactgaa gacaaaaaaa cagaactaat gaaactgaat aaaaatatag
      121 agtgaactag tttctaagag ggattttaaa ctagatttca ttggtaataa aatttggaga
      181 gatggaaaaa cagcacttaa ggaagcagat tttgttgtca tttttgttcc tacaaactat
      241 ggtagtgaat gtgttattga attagtgatg tctgttattt cagaggcatt ggggataatt
      301 aaatccacta tacctgtagg atatacaaaa agtggaagaa aaaaatatta tcaatagatt
      361 ttatttagtt cagaattttt acgtgaaagt agattattaa tgataatctt tatccaatta
      421 gaattattat cgtaggatgt gatgatgaaa gccgtgaaat tgttcaagta tttaccaatg
      481 tctccctgcc agtaaatccg cttagaattt gagccttgaa aatgtgcaat gataaaaaac
      541 aaaaactcgc tcaaaataga cagtttgggg tgggaataga ccaataatgt accagacaaa
      601 cttccatttg gggtgaaaat aggtcaataa agcgatgtta aacatacctt tggagtaaga
      661 atagagggta ataagtgaat ttataaactt tttggtgact ggttgagtgg aaaatgtagg
      721 tacatttcaa tgcgaaaatg tattataacc ccaaacgagg tcaataaaaa tgactcaaac
      781 ttgcaatata accctaaacg ggtaggggat ttatgaaaaa ttgaatttgc cggcgtagga
      841 gctggctgtt ttaagtatga atgaacacag ctttcgggct gttttatata aatagcaggt
      901 tactgttcac aaacagcatt ttgttaagaa agtagtgacc acagcagtgg tggaaagagg
      961 aaatattatg aagaaacagt ataacattgc agtcgccggc accggttatg ttggtttgag
     1021 tattgccact ctgttggcac agcaccataa ggttacagcg gtggatatca ttccagagaa
     1081 ggttgaactg attaataacc gtaaatctcc aatccaggat gattatattg aaaagtacct
     1141 ggctgagaaa gaactgaatc tgaccgcaac attggatgca aaggcagcat accagtctgc
     1201 ggattttgtt gttattgcgg ctcctactaa ctacaatagc cgcactcaac attttgacac
     1261 atccgctgtt gaggctgtta ttaggctggt catggaagta aatcctgatg ccattatggt
     1321 tattaagtcc accattcctg ttggatacac cgcttctgtt cgtgtaaaga ctggtagcaa
     1381 gaatattatt ttcagccctg agttcctgcg tgaaagtaag gcactgtatg acaatctgta
     1441 tccttcgcgt attattgtgg gtaccgatat ggatgatgag cgtttggttg aggatgcaca
     1501 cacttttgct acactgctac aggaaggcgc aatcaaagag aatgtggaca cattgttcat
     1561 gggctttact gaagcagaag ctgttaagct gtttgccaat acatatctgg ctcttcgtgt
     1621 cagctacttc aatgagctgg acacttatgc agagatgaaa ggcttgaaca ctcagcagat
     1681 cattaatgga gtttgccttg atcctcgtat tggtactcat tacaacaacc cttcctttgg
     1741 ttacggtggc tattgcctgc cgaaggatac caagcagctt ctggccaact acgcagatgt
     1801 tcctgaaaat ctgatcgagg caatcgttga atctaaccgt actcgtaagg actttattgc
     1861 ggatcgtgta cttcagatgg ctggttatta caattacgca gaagcaaatc agtatgatgc
     1921 tggcaaagaa aagcctgttg tgattggtgt ttaccgtttg accatgaaat ccaattctga
     1981 taacttccgc cagagttcta tccagggcgt tatgaagcgt gtaaaagcta aaggtgcaac
     2041 tgttgtgatt tatgagccga ccttgaaaga tggagagact ttctttggca gtaaggttgt
     2101 gaatgatttg gaacagttta aggcaatgtg tgattctgtt atagcaaatc gctacgatgc
     2161 ttgccttgat gacattaagg aaaaagtata tacccgtgat atcttccaga gagactaaga
     2221 ttaggagaga gtatttatga ttattacaaa aacaccgttt cgtatgtctt tttttggcgg
     2281 tggaacggat atggaggatt atttcaaaga gaatggcggt gcagttcttt ctactacttt
     2341 tgataagtac tgttatgtga atgtgagaca tctgcctaga ttttttgatt acagcgctga
     2401 gttatcttat tctcgtactg agagagtaac tagcattgaa gatattgagc atcctgcgat
     2461 tcgtaatgcg atgaaaatgc tcgatgtgca tgaaattcgg cttacatacg aagctgattt
     2521 acctgctcgc tctggtctgg gtacatccag ttcttttgca gttggtatgt tgaatgcttt
     2581 ttatgcactg gagggtaagt atgctgacaa gaagaaactt gctgatgaag ccatttatct
     2641 tgaaagagtt ctgtgtaatg aagccggtgg atggcaggat cagattgccg catcttatgg
     2701 tgggttcaat cgaattaatt tcaatgtgga tggttatgag gtgctgcctg tcattatttc
     2761 gcctgaacgt aaaaagcagc ttaacaacaa tctgatgatg ttctttaccg gattcactag
     2821 attttcttct gatgttcaaa aggctaacaa cgtcagtggc acagaggata agagagtaag
     2881 actgaaaaag atgtatgagc ttgtagatga agctgaggca attcttactg ataagaacag
     2941 aaatttagat gatttcggta gacagcttga tgttacttgg agattgaaaa aaggtactgg
     3001 tggagcaatt agtacaggaa gcattgatga actatatgaa aagggtatgg ctgcaggtgc
     3061 tttaggcgga aagttgctcg gtgcaggtgg tggaggtttc ttggttttct atgtacaacc
     3121 tgagaaacag gatgcagttc gttgggctat gcgtgatttg atgtatattc cgtttgaatt
     3181 tgaagatggt ggaactagag ttattcacta cacgccggaa acttatattg aaaaacagaa
     3241 tggataagga gtaacaatgt tagcggaaaa acttaataaa catattgact tattgattaa
     3301 acgatatcct acacttgaga cgattaaaca ggacattatc gatggatacc taattatgga
     3361 ggaatgctat ttgaatggtg gtaaacttct tgtggcagga aacggaggat cagccgctga
     3421 ctcggaacat attgctggcg agttgatgaa gcggtttaag acgccacgtc ctgtaagtaa
     3481 ggaatttgcc gataagttag ttgcaattga ccctgtaaga ggtccggaat tagccaaaaa
     3541 tctcgagtgt ggtttaacgg caattccttt ggtcgcacac gaagcattgt ctactgctta
     3601 tattaatgat gtcgatggtt tgggtgtctt cgcacagcag ctgtttggct ttggtagatc
     3661 tggtgacgta ttccttggta tttcgacgtc cggaaatagc aagaatatta tgagcgcaac
     3721 tgttgttgct agggctctcg gtataaaagt tattggcttg acgggcgcaa agggtgggga
     3781 acttgccacg gtagcagatg tttgtgttaa ggctcctgaa gttgaaacat acatgattca
     3841 agagtatcac ttgccgatat atcattgttg gtgtttgatg ctggaagata aattctttgg
     3901 aaacgaataa aatttggagg aatgttaaat gaaaaagaca attatggtta ccggtggttg
     3961 cggtatgatt ggctccaatc ttgttaagag actggtgaag gaaggctgct gggatgttta
     4021 tgtagctgat aatctgtgga gaggaaagtt ggagtacctg aacgacgaag atgggcatcc
     4081 tgtcatcgac ctggatactc atttcttcaa tgctgatctg actgattatg agcaggctaa
     4141 aaaggttatt ggtacgactg agtatgtagt tcatctggct gatgttgtgg ctggcattga
     4201 ctatgtgttt aagaaccagg gcgaattgtt ccgaatcaat aatctgatca attctaatgt
     4261 ttttgattgt tgccgtaagg ctggcaagga gaaaatcaag ggtgttatct atgttggtac
     4321 cgtatgctcc ttccctctga ccagacagaa tactttgaat cctgagcctc tccgggaaga
     4381 ggagttgttc cctgctcttc ctgagtctgc ttatggctgg agcaaactga tgggccagtt
     4441 ggagatgggg tatttggaaa aagaaaccgg aattccgtgc tgtatcctga tgcttcataa
     4501 tgtttatggc acacctactg attttggcga acgtagtcag gttattcctg ctctgattcg
     4561 taaagcaatc aataatcctg aagagccttt taatgtgtgg ggtagcggag agcagggacg
     4621 tgctttcatt catgtcaatg atatcgtaaa tgcattggtg ctcgccctgg ataagggttg
     4681 gggtcatggt catattcaga ttggcccttc tcactgtact tccatcaagg aaattgcata
     4741 taagatcatt gaaatttctg gcaaagacat caagcctttc tttgatactt ctaagcctga
     4801 aggtgataag gctcgttgcg cagattattc taaggcgaaa gagattttgg attggaagcc
     4861 tactgtttct ttggaagagg gtcttcgtga atcctacaat tggattgaaa agcagattaa
     4921 gggttaataa gtatataagc tgttcttcct ccgtgtaatt agaggatgcg gaggaagaac
     4981 atatgagtaa ggatgtactg agatggaaaa gaatatgtat aaaagaaaac gtgatttcac
     5041 tgtgtgtgga atagtaggtg tttgtgctac tacaaagaac gagtttgtaa gggaagtttt
     5101 ttccatgcca acatctccgg taagtgcgac tgttgatctc gtgggagtgc cggccattat
     5161 ttcggcaaag gaaaatccca aaattgctga gatgtatagc caggctacaa tggctgcaat
     5221 agatggtatg cctattgtta aaattggacg gcgcaaagga tttacctgtg aacgatgtgc
     5281 tgcaccagat attatgggaa tggtttttga agaaggcgtt agacagggaa aaacacatta
     5341 tttctatggt ggaaaagata acgaagtgtt aaataagctt cgtgcaaacc tagaaagaga
     5401 ttacccggga atcaaaattg ttggaatgta ttccccacca tttcgtccgt taaatgaaga
     5461 agaggatagc aagatttgcg aggaaatcaa caacttaaat ccagattttc tgtgggttgg
     5521 tattggtgcg cctaagcaag aaatgtggat gtgggaacac cagaagaaga tccataacac
     5581 agtaatgttc ggtgtaggcg ccggctttaa cttttttgcg ggcactttgg aaaaagctcc
     5641 tgaatggatg gaaaaagcta gtctagagtg gttatttaga ctggtaaagg aacctcgtag
     5701 actatggagg cggtatatct tgggaggtat aaagtatatc tactatacta ttgaatctaa
     5761 gtaggggggc aataaaaata gatgaaaata cttatagtag ttacaatgac gaataacttt
     5821 ggtaagaagg ggtcctataa ttctcaggaa ataggattag ctcgagggtt agctgaatta
     5881 ggccacgaag ttattgtata taaaagtgta aaaagtgatg aggaagttgg aacagagcaa
     5941 ttaagcaata cggcgatgat tcactatatc aagtgtcgtt atgtaggatc aaatggattc
     6001 ttatcaacaa agaattttga taagaatgcg gatgttctaa tacaatttgc ggaccttcaa
     6061 ctatcagtac ctcatgtata ctcatggact gtaaaaaatg gaataaaata tataccttat
     6121 aatgggataa cgcgaagtca aagtggcaat aaatatataa agacaattat tgaaatattg
     6181 gcgcatcgaa attataaaat ttataagaaa aatgggtgtg ttgccaaaaa tgatcgagtt
     6241 tctgaaaaat tagtacaatt aggagtaaat aaagtgaaac ttgctcctgt ttgtgtggat
     6301 tttgaacagt tacataagaa ttacgaagca atagatttta ctactctcag agaaaaatgg
     6361 aatattcctc gcgatgttcg tgttatttta tatgtaggac ggtttgaaaa ggataaaaga
     6421 ccgttagata tgattagttt atttaataaa cttgatgagc ataattattt gctgatggta
     6481 ggaactggtg ctttactcga gcaaacgaag cagcagataa atttacttgg actgcagaat
     6541 agagtaaaaa taattgagcg tattcctaac aaagatattt gggagttata ttgcttatcg
     6601 gatgtatttg taaatctaaa ctatactgaa atttggggta tggttttgct agaggcaatg
     6661 tactatcgcc tcggaattat tgctgttaat gcacctggcc cacagaatat tattttggat
     6721 aatagatatg gaatgttggc taaatctgac gatgaaattt tgacattctt aaaagacgga
     6781 cgatttacca acgatatcat agatgcagca tatcagcgtg taactacaga atttagatgg
     6841 tcaaagtcgg gagaaattat gcttagcatg ttggaaggtg ataaatgata tggaagtaac
     6901 gaaattaatg gcattgagga atcgatatgc tttaaacata gttgataatt gcacgagaaa
     6961 aatagccaaa attcttggtt gttgtattgg taaaggtgca cagataggga actctgttga
     7021 atttgttcat aattctgtag gcactgtgat tcattctgac acaatccttg aagatggtgt
     7081 taaagtttat cagaatgtga cgtgtggttg agcagatagt aatgaacgta tcatcattcg
     7141 agtaaaaaag aacgctgttt tatgtgtggg tgctaaaatt ctttgtaaat ctaacacaga
     7201 attatgcgtt ggggaaggtg ctgtagttgc tgcaaataca gtggtattga ggtctattcc
     7261 accctacgaa gtgtgggggg tacctgctaa aaagatgggg agataaaaca tgaatgcata
     7321 taaattatac aaagtagaac gatggtttta cttacacaaa atggagtttt ttgccaagat
     7381 tgtaaaggca ctaatttatg tacttcataa tagctcaatt tcatatcaaa cagaaattgg
     7441 agataattgt aagttcttat acggtggcat aggttgtgta attggaaaag atacaaaaat
     7501 aggtaatcac gttataatcg gaacgaatgt actgactggt ggtagatcca ataagcctgg
     7561 tatgcctaca attggaaaca atgtatatat cgctactggc gcaaaaattt taggtaatat
     7621 cacaataggg gacaatgtaa tagtaggggc taatgctgtg gtcattaatg atgttgaacc
     7681 taattgttcg gtaggaggcg ttccggctag agtattgaaa aaagatattg atgtaaatga
     7741 ttattgtagt ttgaatcgtt actaaaagga gagattgtat gttgtatttt gttattcctt
     7801 taatgagtgc gaaggcagca aacaattggg agatggttag taccttattt aaccgaacat
     7861 tatggtcgtg ttataatcag actgatcctg atttcaaaat cattgttgct tgccatgaaa
     7921 ttcctaaact cacgcatgaa tatgatgaca gagttgaatt tattcaagtt agcgaaaagg
     7981 atgcccctat tcccacaaat caagacgaaa aaatgattga taagggttat aaaacacatg
     8041 ctctcgcaat gcgtttgcga gagttgggcg gcggtttcgc tatgatggtt gacgcagatg
     8101 atcttgtaag ttctcatttg gcggaatatg tgaattccca tccccaggaa aatggatttt
     8161 atgtcaaaac gggatacgtg tactttgttg gagacgatta tatgaaggtt ctaccaaagt
     8221 tctcatcagg aagtgcgtgt atagtaaaat atgcagtgga agacctgcct gaggcatatc
     8281 cggaagttat gacagcaaat tgtgatgata atccatggat cattagaaaa cgacatgggg
     8341 gcgttgttcc agcatgtgaa gagcaagggc gtccattaaa acctcttcca ttcaaagcag
     8401 cagtatatgt attaggaaca ggggacaatc actcgcttta tggtaaaacg acaaaatatc
     8461 agacgaagat gcgagagata ctggaaatgt ttatagcaaa acgtaaaatt aaaggaaaac
     8521 tgaaagcaga attttctgtt gattggttat aaggtgagta ttatgaaggt attgatgtgt
     8581 gaaagccaaa tgccagagaa ttggtattcg tttgatcttt ctaaagaact taatcaatat
     8641 gttgatttaa cattgctgtg taagcgtgat gttcctcctt tagatcctaa aattaagagc
     8701 aaacatattt tgtatcataa aagtacaaat aaagtgtttt cggcacttgt ttttctgaaa
     8761 agcttgttct taatttggaa ggaaataaga tttggtgatt atgatattgt tcatttgcaa
     8821 ttgatgaaaa gtctcaaata tgaaggcgga ttgtacgttt tatgtaagag acactataaa
     8881 aaattaatta tgacagttca taatgtgtta ccacatgagg ttcgtgaaaa ggatcgccaa
     8941 gagcacttta atatatattc aaattgtgat ggtttcattg ttcacaatcg agtaacagaa
     9001 cagcatttat atgctgaatt cccagaatta aatgcaaagt catatgtttg tccacatgct
     9061 atctatggag atgttaaaca tatatctaga gcgtatgata aagataagtt tgaatttatc
     9121 ttgtttggac aaattcgtcc atataaggga attgatgtac tgcttaatgc aatcacagaa
     9181 cttccaatgg atgtaagaaa gaaagttcat tttaccatag caggtcaaca acatcctaaa
     9241 caggatagta ctgactataa aaaaatgatt gaagatttaa aggtttcaga ttgtgttcta
     9301 tataagccag agagaatttc tgatgaagaa atggacagta tgttcagaaa tgcagatgcc
     9361 gcaatatttc catataaaga aatctatgga agtggagcgc ttcttatggc atacacttat
     9421 tgcttaccaa ctattgttag taatgtacct gcttttatgg aagaaaccga ggaaggaaag
     9481 atagggttac ttttcgaaaa tttgaattct aagagcctag cagacacaat tgtaaagtat
     9541 gtgaacctta gcccagctga gaaaagcaaa atagaggatg atatatatga gcttgtaaac
     9601 cgcaagtata cctgggcggc atctgcaaaa ctcacatacg aggcatacaa acaaattttg
     9661 aatgagtagt aaaacaatta gaaaggaata ataattaaca tgcagattag taaatggaaa
     9721 atcatacagt ttctaatggt ctttctaatg attatctcat caagcatatt gaagcctttg
     9781 ggcggctggt tgggagaaac gatgaacgtt agacttgtga cagtcattct agcaatagta
     9841 tgtttgttaa tagcctcatt accagcattt agaaaaaata gatatttaaa tttctgcact
     9901 ttactgattt gtgttgttat aatagcagct tacatctact cagataagac aatatgggtt
     9961 aaatctaggg aaagtatgag tttatactat gtattattgg cgtatccatt gttctgtact
    10021 ttaattaacc aagcgtggaa tttggattca atgctaaata cgatatgtgg tgtaacattt
    10081 ctttcataca ttcttagaac atctatttca cttattcaga aattttcagg agtacttctc
    10141 tatgaaaaca tatatcgtga gtgtgccccg gaaaattggt atcgtggagg ctttttaaga
    10201 attaatccac catgttttac aattataata ataccgattg ctttgtatat gattgagcga
    10261 caagttgtag ctagaagaaa agtaaaatat tatttactga ttgctagcgc actggcatat
    10321 tctgctgtta ttcatggttc tagatctgtc cttgtctatc aaattattgt acttggttgc
    10381 tatatccttt ttaagaaagg ttcttcgaaa agaaaaattg caatgtgggt tttagctggg
    10441 ttgcttgtag tcatatttat taattcgacc atgttctcaa catttgttga aactttcaca
    10501 aacagtacag gtgaatatgc aggatctgct gaatctcgat ttgcttcagt ttggtttttt
    10561 gttcctaaat ttttgcaatc gcctttgttc ggtactggta ttcttactgg tgaggaattt
    10621 acgtttatat taccaggagg agttagagga cacctatctg atattggatt tttctataca
    10681 attactcaat atggtgtagg tatacttata tttgatattc tgtttattat aaaagggttc
    10741 aaaacagcat tacttgcatc aaaagtgggt atgactggct atcaatattt agcatttgga
    10801 attacattaa gtgttttgct aacagggatt aatatagact ggttttatcc gatatttact
    10861 ccagctattc ctgtatgctt agcggttata gaatacatct atcaagaatg tagaagtgtg
    10921 gctaacatag aataggaaat attaatagga gtgcatctca tgaataagaa agagatttta
    10981 tataacatat tgaagaagta cgaaaattta ccatatgtgt ttaggaacat gtttggtcat
    11041 aatataagta aactcgtatc ttggaaattg actgctgatt gtgcaacagt agaagaaaat
    11101 tctgaagttt atgcagcgtt tcaattgcgc tcaaaggaca tgtctgatgt tccagtgatg
    11161 ggtttagtta attgttgcaa agatgtagca attgtaatgc aggggccact atgtttaaag
    11221 gatgatttct cttataatac tctggcctac tataagaaat gttatccaaa tgctttaatt
    11281 attctttcga catggaagga cgaagatatt aaggcattga aaaggataga ggatcttgga
    11341 gttatagtta tttgtagtga gaaacctgaa aagcctggac acttaaatat taattatcaa
    11401 gttggaaaca ctttgcctgg aatagtcaga gcaaagcagc ttggtgcaaa gtatgtttgt
    11461 aaaactagaa cagatcaaag aatataccat ccaaatgcaa tggcgttttt ctgcagttta
    11521 cttgaacagt atcctgttaa taacgaagat gattggaaat taatacaaaa tcagcgatta
    11581 ctgttactat caatgccgta tggagacatg tttttcccgt attgtttgtc agacttcctg
    11641 tattttggtg atgttgatga tatgataaac ctcttctcga ttcctagtga tgtgagagaa
    11701 aagggagctg tgtctagagg agtctcaaga agggagatat ctgaaaataa tttagctcca
    11761 gaagttcagc ttcttcgaag ctatatttca cgaatggggg gcaatgagga gtgctctatt
    11821 agggcatatt gggagtttgt aaaaaaccat gttattacta ttaacaaaaa tcaaattgat
    11881 ttgtactggc ataaatatgt aggtcgttat tcaaataata ctatttatgg tacgtattat
    11941 attgatgata gccaagatgc acttcactgc tataattttg attttataaa ttggttgaat
    12001 ttgtacactg gaaaatacga atacaaagca aaatatgaaa aatatatgga ctttgtttgg
    12061 gaagagtaat taacttttat agtaaaaagt actttaggga agggcatatt atgaaaacag
    12121 taattatggc gggcggacga ggaagccgaa tttcggaatt gtttccggat attcctaagc
    12181 caatgattcc tatagatggc attccagttt tagaacgaga attgtgttcg cttagagatc
    12241 aaggctttaa tgatattatt ttgactgttt cctatttgca tgaaaaaata gaggagcatt
    12301 tcggagatgg ttcaaaatta ggaattaaaa tcaaatattt tattgaaaaa actccacttg
    12361 gcaatgcagg tgcattgtat tttttgttgg atgaactggg tgatgaaccg tttttacttt
    12421 tgaatgctga tgcagtattt gatgttgatt tcaggcagat gattagattt catgttgaga
    12481 ataacgcaga tgttactttg tttacgcatc ctaactcaca tccgtatgat agtggattaa
    12541 ttatatccga tgacaataag aaagtgcttc aatggttagc gaaggaagat caaagacctg
    12601 aatattataa aaaccgagta aatgcaggtc ttcatgttat aaatccgaag gtgttgagag
    12661 aaaatccatt aatggaaatt gtaaataaat caatagttga aggtgggggg ctaccataca
    12721 aaattgattt agatagaatg attctgaaac cattggctaa cacagggcgt atgtattgct
    12781 acgattctcc agagtatgtt aaagatatgg gaacaccgga ccgatataga gctgtaatta
    12841 aggactttca aaagggaatt attgttggta aaaatctatc caatccacaa aaagcaatct
    12901 ttttagatag agatggaacg attaataaat acgttggttt tttgagaaat gcagaagaat
    12961 ttgagttaat tgatggagta tcggaggcaa ttaagattat taattctcta ggttttctgt
    13021 gtattgttat aacaaatcag cctgtaattg caagaggtga agttactgtt gatgagctta
    13081 atagtattca caataagctt gaaacaatgc tagggcatgc tggtggattt ttaaacggta
    13141 tttattactg tccgcaccat ccccataaag gatatgcagg agaggttata gaattaaaaa
    13201 ttgattgcaa ttgtcgcaag ccaaaacctg gactgttatt agaagcatca aaggatttta
    13261 acataaacct aagtcaatca tttatggttg gagatagtga aagtgatgta attgctggaa
    13321 aaaatgctgg ttgtaagaca gtactgatta cagaagacat caccttgcct gctaattatg
    13381 gccaagacta tagtgtctgt tcattgttag aatttgttaa aaatcattta tgcactatgc
    13441 aataattggt ttagtgaaac ttgtaaaagg agtaattaga tgaaggtatt aattccagca
    13501 gcaaaagttg ttgaaaaaga attgcagagt ataggaatgt taccagcaat tatctatcca
    13561 attaataaaa aaattgtctt tgattatttg tataatcagt ataatgcaat ctgcgattca
    13621 ttggatatta tctgttatga aaaagcggat aaggttcata gaagactgaa aaactatcat
    13681 gattccagaa ttcatatatg tgaccttcct gaattagggg atctagggca aaccgtgtat
    13741 tttgggttat cagatgaaga tgagcctgtt ctaattaact tcgcagatac aattgttatg
    13801 gagagtatgt ctgatataga aatggataca ttctattatt ctgaagattt tgtgtcgagt
    13861 acttggacat actttaaaat taatgaagga ataattactg atatccacga taaagaggaa
    13921 gcttacgtat ccgataaacg tgaaaaatta tttattggcg tatttatgtt ttcttcgtca
    13981 aaacttctga agagttgctt aagagaggca gttgagagtc ggaaacaggg gggaataaac
    14041 tcattttatc gggctttaca gctatacagc gaacgttgtc cgctaaaggc cgtaagaaca
    14101 gaaaagtggt ttgatattgg acacatcgat aaatattaca atacaaagtt ggaagttaaa
    14161 gcacgagagt ttaatcatat ctctatagat aaagatcgag ggatattgaa gaaaactagc
    14221 gatgacaagg ataaatttat tggagaaata aagtggtatt taaaacttcc tgctgatatt
    14281 gaatatgtaa gacctagaat ttttgattat tctacatctt acgtagctcc ctatgttgat
    14341 atggaatatt atgcatatca tacggttcat gagttatact tatatggtga tttaaaatat
    14401 aaccagtggc atgatctgtt tagccgtata aagtttatct gtagcgattt taagcgatac
    14461 accttaaaag atgaaaagat tagagagtca ttggaagata tgtatttgcg aaaaaccatg
    14521 cagagatttc aaaaattgtc tgaagataaa agattttcag ctttttttga gaaacaaatt
    14581 caaattaatg gtcgtaagtt tatttcttta accggtgtaa ttgaacttct aaagaatctt
    14641 atcccaagta tgctctatga tgtaaatgaa ttttgtatta tacatggaga tttgtgtttt
    14701 gctaatatga tggtagattc caattgttcc tttgttaagg ttattgatcc taggggaaaa
    14761 tttggaaaat atgatattta tggggacagc agatatgaat tagcaaaact atttcatagt
    14821 ttagatggta aatatgatta tatcatcaag gatctattta ctgtagaata tgataccgag
    14881 tgtgcagtga tagattatac aattaatacg cgtgtaagag attacgattt atttgaattg
    14941 tttaagtcgg tcttttcgga cgagattaag gacgattttg ataagattgt attgatagaa
    15001 gcgttgctat ttcttagtat gattccatta cacggtgaaa gttttgatca ccaaatagtg
    15061 atgcttgcta caggtatgga acttcttgca agagtaagta ataaagttat actctaatat
    15121 aaattgtgcc ttaatgaata acggttacaa tactgtttta gcaatgtagg cattaaggca
    15181 cgattatatt aattaataca atagaaggga agagaaaaat gtatagtgaa tattcttttg
    15241 tgtttgatat cgatggcact ttgtgtccga taaagaagaa ggatgagcga tatgaggatt
    15301 tggtgccgta tgagaatatg gttgaaaaac tgagattcta taaggagaat ggtgctaaaa
    15361 ttacattgtt cacctcacgc aatgttaatt cctataaagg aaatttggga atgataaatg
    15421 ctaatactgc aaaagtgctc ttggcgtggt tggataaatg ggacattccg tatgatgaaa
    15481 tcctgtatgg taagccttgg ccggggcata aaggcttcta tgtagatgat cgaacggtta
    15541 gacctgatga gtttttgaga tgctctgtag atgaattaaa tgaaatctgc aacagttcaa
    15601 aaaaaagatt ctaaataagg aggttagttc tatggaaaaa aagatgacag ttatcattac
    15661 gatggcaggt ttgggaagtc gttttcgaaa aattggttat gcggttccta aatatatgat
    15721 taatgccaaa ggaagaactt tatttgattg ggccatggat agtttggttg gatacaataa
    15781 aaatgttgat aagtatattt ttgttgttcg tcaggaagac aacgcttcag cttttataaa
    15841 ggagcattgt gcttactttg gtattgaaaa cgtagaagtg attgaaatag actatatgac
    15901 agatggtcaa gcaactacat gtatgttggc tattccttat tgcaatcaag acaatgagat
    15961 tatggtttac aatattgata catacgtgga atcaaatgag atgaaatacg aagatatttg
    16021 tggagatgga catattccat gctttcatgc tgatggcgat cattggagtt ttgcaaagct
    16081 taacgatcga aatgaagtaa tagaagtccg cgaaaaagtt cgcatttctg ataattgtac
    16141 tcttggtgcg tattatttct catcggcaaa gctgtataaa gctttatatg aagagtatta
    16201 tgcagactcc tctaatatgg agaagaatga aaaatatata gcaccattat ataactttat
    16261 gataaaaaaa gggttaaagg tgactattag catagtagat gcgaaaaagg ttcatgtgct
    16321 tggtactcca gaagagctca atgtgttttt gaatcaagaa taaatagtgt ttaggagaaa
    16381 atagcagtga ccagtaataa tctaattgta aaaacattaa gcagatataa gaatactcca
    16441 cttcctgtta aggctgcatt atggtttatg gtatgtggtt ttttacagag aggggtttca
    16501 atgattacaa ccccaatttt tactagagtt ttgtctacag aacaatacgg tttatacagc
    16561 acttatctat cgtgggaatc cttattgctc atgattacat cgcttagttt gttcaaagcc
    16621 atgatgaatc tttatgtaaa atatgataat caagaacata ttctttcgac agtatccgga
    16681 ttgactttag tactgactat tttttggttt gttattgcta tttttattaa ggatatattg
    16741 gcagaaattt taggattgac accagttcta gttgtctgta tgttctcata ttttatattt
    16801 caggcagaaa tacaatgttg gtctttacac aaacgatata tatatgatta taagacttta
    16861 ttagtagcta cccttgcagt tactgttggc tcatctgtag ttggctgttt agcggttctg
    16921 tttatatctc ctaccgctga ggcgagagcc ttaatgaatg tcctagtgac tggtgttgtt
    16981 ggcgtggctg tgtatatatc ggtatttaga agatgtaagg attttaagga taatgaagtg
    17041 tggaatttcg cagtagtatt ttgtgtatcg ttgatgccac actatttatc tgaatttatt
    17101 ctgaatggtt ctgataaaat tatgataaat tacttatgtg gttcgtcaga tgttgctatt
    17161 tacagcgtgg catattctat tggtgctcta attaaccttg ttacaaattc tgtcaattcc
    17221 acttttgcac cttatcagta tcaaaaaatc aagtcaggag aatatgattt actatcaaaa
    17281 agagctaatg aagttcttgg tttggtagcg gctatgcttt ttgcattaat gctatttagt
    17341 aaagaaattg tgttgatatt tggtgggcag aaatatatcg acagtgttaa tttaattgta
    17401 cctatatgta tcggtattta tttcaactat atgtttcagc tgtttgctag agtacaggaa
    17461 tactttgaaa agaaactcac tgtcgttatt ccatcggttt tgtgtgcaat tatgaatatt
    17521 gtactaaatt atgtttttat aaagttatat gggtatcagg cggtagcata cacaactgta
    17581 ttctcatata tgtttttctg cgtcatccat tatatttttt atagaaaagt ttgcaaagaa
    17641 ctattggatg gaaaaaacat atatgatgtc agaggaatac ttttgatttc tgcaatcacg
    17701 atagctggtg gcatcctaat ttcaataatt aataaatttg atttcataaa atatttaatt
    17761 atagcaattg cttgtgtcgc tgcaattata tttagaaaac gattgctact tttagtaagg
    17821 agaattatga gcaaataaac atttgctcat agagagatag agaatgatag atattatttc
    17881 tgctataatg gtaaatgtat taacagcatt atatcagccg ttcttatttg ccgtattaac
    17941 agcgtatctg tttatgtact ggtatctatt tatagttgat aactctcgag atcctaaaaa
    18001 cttaaaagat tctgtattaa tctggttgag atattttaaa gcatctagta aatttcgcag
    18061 actctttttt cttgcgtttt attttacttt gatattattt aaaaccttgc ttaatcgaaa
    18121 tatgtggatg aacccgcttt caaacgttat tggacagtgg tcattatata ttatagataa
    18181 tgctacagga gagagcagat tgacaacaga atgcgtggag aacacgatgc tgtttatccc
    18241 tttttgcatg ttattgttat ggtggcgtga cattaaatgt gatattatac gcaccatcta
    18301 tgttgggatt aagtatgtgt ttttattctc attaagtata gaatttacac aactgttttt
    18361 taggcttggt acatttcaac tatcggattt gttttataac accctagggg gcttaattgg
    18421 tgctttgcta tattggctat tctaccgatt aaataagtat atagacttaa aatagaactg
    18481 ctttgaattt taatatggtt aatatttttc tgcagacagg attgtgtaat ctcatcaaga
    18541 atactttaca tgccatctat ttttgctaaa acattgttgt tatatcttca agaggtaggg
    18601 aattttattg ctttaaatga ggtcgagcga attaaaaaat tggtaaaata tataacatgg
    18661 tggtagttat ggtaaaagtg tttgatataa ttaagttttc ggatttcaga ggatatactg
    18721 ctgttgcatt ggataatgag actcttttct cgattggtat tgagtttcac attaagcaaa
    18781 taaatcaagg atttagtcat aataaatata ctatgcgagg gctacattat cagagtggag
    18841 agcatgctca aggaaagata gtgatgtgcc tacatggtga aattatgaat atcgcacttg
    18901 atattcgaca aggatcttcc acttatggat gtgtgtatgt caacacattg agtgaaagca
    18961 atggtaaatg tgcgtatatc ccacgtggat atgctcatgg ctatcttact atggaggaca
    19021 atacgctagt tcagtggtgc gtagataatg atttcaattc taatgctgcc aaatgtataa
    19081 gttatgaaag cgtagatatt gaatggccgg tcagtcggca gtgtttcgtt ataagtgaga
    19141 aagacaaaaa tggggaaaga ttaagctttt gattaaaatg agctgtggag aaaagaagga
    19201 tactggaaaa taagaagaac aataaccgag caaatgctcg gttatttcta tgaggaatac
    19261 ataaaactaa atttagatat agatatttat tcacgaaaaa ctaatatt
//
LOCUS       DACEZN010000042        19144 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEZN010000042 DACEZN010000000
VERSION     DACEZN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.042"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..2175
                     /locus_tag="IXH60_001132"
     CDS             <1..2175
                     /locus_tag="IXH60_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1990085.1"
                     /translation="YWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVF
                     LKKIKKSKTGQEAANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALID
                     LLDEYVVIYGESAFDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVT
                     FALGLNDQVFPDRKETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFL
                     SGTDRLYLSYAQSYDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRS
                     GINTVNRIYRLALDEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEK
                     MAEELYGKEIYTSISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDR
                     FFKLLFSNQLSLVNMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQ
                     KVTWALQKQSKNTGMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVA
                     SEQEDTWLSVVDYKSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGS
                     YYLHVHNPVLTETDDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKN
                     AKDEYQKGSFSKDKFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIAC
                     EFCPFRSICTFDVMLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            2172..5894
                     /gene="addA"
                     /locus_tag="IXH60_001133"
     CDS             2172..5894
                     /gene="addA"
                     /locus_tag="IXH60_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1990086.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            5963..6304
                     /locus_tag="IXH60_001134"
     CDS             5963..6304
                     /locus_tag="IXH60_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1990087.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            6613..7656
                     /gene="pheS"
                     /locus_tag="IXH60_001135"
     CDS             6613..7656
                     /gene="pheS"
                     /locus_tag="IXH60_001135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1990088.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            7661..10081
                     /locus_tag="IXH60_001136"
     CDS             7661..10081
                     /locus_tag="IXH60_001136"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1990089.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(10251..11261)
                     /gene="trpS"
                     /locus_tag="IXH60_001137"
     CDS             complement(10251..11261)
                     /gene="trpS"
                     /locus_tag="IXH60_001137"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1990090.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            11515..12252
                     /locus_tag="IXH60_001138"
     CDS             11515..12252
                     /locus_tag="IXH60_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990091.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            12266..13081
                     /locus_tag="IXH60_001139"
     CDS             12266..13081
                     /locus_tag="IXH60_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1990092.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            13094..13741
                     /locus_tag="IXH60_001140"
     CDS             13094..13741
                     /locus_tag="IXH60_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1990093.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            13757..14413
                     /locus_tag="IXH60_001141"
     CDS             13757..14413
                     /locus_tag="IXH60_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1990094.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            14575..15396
                     /gene="racE"
                     /locus_tag="IXH60_001142"
     CDS             14575..15396
                     /gene="racE"
                     /locus_tag="IXH60_001142"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1990095.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            15399..16754
                     /gene="rph"
                     /locus_tag="IXH60_001143"
     CDS             15399..16754
                     /gene="rph"
                     /locus_tag="IXH60_001143"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1990096.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            16755..17273
                     /locus_tag="IXH60_001144"
     CDS             16755..17273
                     /locus_tag="IXH60_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1990097.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            17380..17880
                     /locus_tag="IXH60_001145"
     CDS             17380..17880
                     /locus_tag="IXH60_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1990098.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(18160..19080)
                     /locus_tag="IXH60_001146"
     CDS             complement(18160..19080)
                     /locus_tag="IXH60_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1990099.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         6386 a         3313 c         4097 g         5348 t
ORIGIN      
        1 tattggactc gtgaaaaaga ctggcagttt gtggcgtatg attttgaagc agaacagatt
       61 gaagacgtac agtatttgga agaacagtca aatacgatta gacgattatt tcagaaagct
      121 gtaccagtat ttttgaaaaa aataaagaaa agcaaaacag gacaagaggc cgcaaatgtt
      181 ttttatcaat ttttgatcca aagtggcgta cagcaacaat tattgttttg gcgtaatcag
      241 gaagtagaca gaggaaactt agaagcggca agaaaccatg agcagacatg gggagcgctg
      301 attgatctgt tggatgaata tgtagtaatt tatggtgaat cggcttttga ctggtcactg
      361 ttccaagaaa tcattacaag tggtctagaa aatctaagtt atggtaaaat ccctaccgca
      421 atcgaccaag tgcgaatcaa tcgtttggaa ttagttcgac caaatcaagc tgccgtcact
      481 tttgcattag ggttgaatga tcaagtgttt ccagaccgaa aagagacaaa aggattgttg
      541 tccagtgaag aacgagaagt attaaatcag gccttgccag atggacagtt tttattcgac
      601 ccatcaaagg aaagtatctc ttttgagcct ttccaagcct atcttgtttt tctttccgga
      661 acagatcgat tatatcttag ttatgcacaa agctatgaca cagatggttc gctgaagatg
      721 tctccttatt tgcgtagaat cacagaatat ttatctgttc ccgtcgagag aaaagagcat
      781 ttgacgatcg aaagcgaccc taattgttat gtagggactt acagaagcgg aatcaataca
      841 gtcaatagga tttatcgatt agctttagat gaaaaaaaac ctgtaccaaa gcactggcaa
      901 aagctcaaag aattagtact gaattcgtca tggcgaaatt ttgctttaca gatttttgaa
      961 agtcaggaac atttaaatgt tcctgtttcg atgccagaaa aaatggcgga agagctttat
     1021 ggaaaagaga tttatacttc aatttcacgt atggagaatt tttataattg tgaatacaaa
     1081 tacttcatgc aatttggttt gaaattgaaa gaacgaactg tttatggtct aactccagca
     1141 gcaactggtg atttttatca tgaatcattg gatcgtttct tcaagctact gttttcaaac
     1201 caattatcct tagtgaatat gtccgatgcg cagcgtagag actttactga aaaagtgctg
     1261 caggaagtat tcggagaact tcggtttgag atattggata gttctgcaag aatgaactat
     1321 atccgctacc agttaggaca gacgattcaa aaagtgacat gggctttgca aaaacagagc
     1381 aaaaatacag gcatgcatcc tcttcagaca gagattctat ttggtcagat tgctggagca
     1441 aaaggtatca caggtctgga attaccgttg agtaatggtg gaaaactcca tgttcgaggg
     1501 aaaatcgatc gaatcgatgt agcttctgaa caagaagata cttggctaag cgtcgttgat
     1561 tacaaatcta gcggacgttc attcgatgtc acagaagctt attatggcat ggctatgcaa
     1621 ttattgacgt atctggacgt agcattgatg gatgcggtac gtttgactgg aaaggcggct
     1681 gttaaaccag caggatcata ttatttacat gttcataatc ctgtattgac ggagacagat
     1741 gatgtagaga agaatacatt gaaaaaattt agttatgatg gcgtttttgt cgatgatccc
     1801 gctcttttag aagtactaga tcattcactg gaagcaaaag aacaatcact gatctatcca
     1861 gtgaaaaaaa atgcgaaaga tgaataccaa aaaggtagtt tcagtaaaga caaatttttc
     1921 acggaagaag aactgcaact attcatggcg cataatcggg aaaatatgcg gttagctgga
     1981 gatgcgatta cttccggaga aatcaaacta aatccagctt ataaagataa acagcggatt
     2041 gcctgcgaat tttgtccatt cagaagcatc tgtacgtttg atgtgatgct gaaagaaaat
     2101 aattaccacc gtctggagaa aatagataag gaagaagcac tgcgtagaat attgaaaagg
     2161 agtgaagaag agtgaatcaa attcctttaa aaccgcaaaa tgaacgattc acagatgatc
     2221 agtggcaagc gatttttgat caaggagata atctattagt ctctgcgtct gccggatcag
     2281 gtaaaactac agtgcttgtt cgaagagtga ttgaaaaact aaaaatgggt tttgatatcg
     2341 atgaattatt gattgtgaca tttactgaag ctgccgcaag agaaatgaaa gaaagaattc
     2401 aagaggcatt acaagaatcc gtcaatagcg aaagtgaccc tgtacgtaga cagcatttta
     2461 ctaagcagtt agttttattg cccacagcga atatctcaac cttacatgct ttttgcctga
     2521 cagttattcg acgttattat tacttaattg atattgatcc agtctttcgc atgttaacag
     2581 atgaaacaga aacgatcttg atgaaggagg atgtgtggga cgagctgaga gaagcacttt
     2641 atgcagaaaa tgatgaacga tttttccaat taacaatgaa tttttctaat gatcgttcag
     2701 atgacggctt gacgaatctg gtgttttctc tttatgagtt tgcccgtgcc aaccctgacc
     2761 cccaaaaatg gctagaacag ttaagcgata attatcgttt gcctgagggg ctagcgaaat
     2821 ctcgattata tcaaaaacaa atccgtcctt tagttttggc agatatatac caatgtgtac
     2881 agttatatga acagatgact cagttagcac aaggtgaagg attagaaaaa atgcatgagc
     2941 aagtagctgg agaacaacag cagatcaaga atatctatga agcgttctcg caagatcggt
     3001 tagaagaagc ttatgctggt ttggaacaac taactttttc aacttttaag agcagtcgga
     3061 aagcagaatt gaaagaaatt tccaatgaag tcaagggcat gagggataaa gcaaaaaaac
     3121 tgattcagca gataagtaaa agttattttc ctgtttcccc aagccaaatg gaagaattaa
     3181 cggacaaagc actaccgtta gtcgaagaaa tgacaaaagt cacgcaaagc ttcatggacg
     3241 gtttcagtat gcgtaaacgg gaaaaaggtg tgctggattt caatgatctg gaacatttag
     3301 cgttgcagat tctcacagaa aaaacgaaag atgtatggct gccaagtgaa gcttcaaaac
     3361 attatcgaaa aaaattcaaa gaagtaatgg tagatgaata tcaagacgtg aaccaattac
     3421 aagaagccat tttatactgg ctaagagaac ccgatgatac aaaaggaaat atgttcatgg
     3481 ttggggatgt caaacaatcc atctattctt tccgtttagc agatcctagt ttatttatcg
     3541 ggaaatacga gaatttctca aagaaagaag gcggacgtcg aatcgttttg gctgagaact
     3601 tccgctctag aaaagaagtc ctttctttta cgaatcttat ttttgaacaa ctaatggatc
     3661 cagcagtggg tcaaatcaat tatgacgaag cagccaaact gatccaagga ttttcagact
     3721 ttccagagaa tgaacagttt gagccggaaa tcatgatcta tgaaaaagaa caggaagaat
     3781 cagagattga gattccgacg gatgatatac tggaagacaa aacagaaggc gaacttttta
     3841 tgacaggact aaaaattcgt caattaattg attcctcctt tatgatttat gataaaaaat
     3901 caaaaaaaag tcgtccgata gagtacaaag acattgttct cttgacacct acaaagaaga
     3961 acaacttaac gattttagag attttcaaaa cattggatat tccattagag atgaatgatg
     4021 ctcaaaacta ttttcaagcg acagaaattc gcacgatgat ctcgctgcta caattaatcg
     4081 ataatcccta tcaagatatt ccgcttgcgg ctgtattgcg ctcaccgatc gtgggtctga
     4141 ttgaaccaga attagccagc attcgattag ctaaccgtgc acatacttac tatgatgctg
     4201 tgcttgctta tcaagcatca aacgaagatg aattagcagc taagctggaa cattttggca
     4261 agcaactaga gcattggcga gaacttgctc gccgttcttc gatcactgac ttgttatggg
     4321 atatctatta tgagacggga tatctagagt atgtggtcgg tctgccagca ggggcacaga
     4381 gacaagccaa tctttatgca ttggttgatc gtgccaaagc ttacgagcaa agttctttca
     4441 gaggattata ccagttcgtg cgtttcattg aaaaaatgca agaaaaagat aaagacttag
     4501 cagaaccagt catttcaata gaagataatg ctgttcgcgt tatgacgatc catgcaagta
     4561 aaggcttgga attcccagtt gtatttttgt tagatatgac aaaggagttc aacttacaag
     4621 acttgcgcaa tcggtatgct tttgaagaaa aacttggagc aggaattcgc tatatggatc
     4681 ctgaaacacg agtgttatat gatacgttac cgtttcaagc catcaaattg gcaaagcaaa
     4741 acaaattgtt gtcggaagaa atgcgtaaat tgtatgtagg attgactcgg gcagaacaaa
     4801 agcttttcat tgtcggttcc tataaaaata aagagcaaat gatccaaacg tggtctgaag
     4861 cagctgatca tgaggagtta gtttttgatc ctgcgctacg tttgaagggc agaagcagct
     4921 tgatgaattg gattggttac ggattgatcc gtcatcctga aatgcagaaa tacttagagg
     4981 aagaaatttc gacttctctt cttcaacatt caaatgcaca gttttctatt tcatggatga
     5041 atcagcaatc gatcattgaa caaagacagt tgcttgctga aaaagaactg gttaacttag
     5101 atcagcagat gaaagaagat gaaactttat tagcagattc gttacaaaaa cgtctggcat
     5161 atgaatatcc ttatcaagct tcttcacaaa ccacaagtta tcaatctgta tctgaaatca
     5221 aacgattatt cgaagatcca gatgatacac aagaatcacg tttgactctg gaaagcagtc
     5281 agaacaaagc agcatcccgt caatttcgtt atacgcaaga acaactagca gaaccaaaat
     5341 ttttgcaaaa agatcggcaa gtgtcagcag ctgctgttgg aactgctacg catgctttgt
     5401 tacaattact gccattagag atgccaacta cagagagtat ccatcaaaaa ttacaggaat
     5461 tggtaaggaa acggttagta gacgaaaaag tcgcgaaaaa agtagatgtt tcttctatta
     5521 tatggttctt tcaaacagaa cttggtcaac agctgattgc caacaaagaa aacgtaaaac
     5581 gagaacaacc tttttccatg ctgcttcctg ctgacgaagt atttcaagac tatccaaatc
     5641 aagaagatga actattaatc cacggaatcg tcgatggata cctagaagaa aaagatcatc
     5701 tcaatatcta tgattttaaa acagatttta ttttacatcc ggatgatcca gcagaaatag
     5761 atgcaatcgt ccaaaaatat caaggacagc taagacttta ccaacaagca atgtcagaag
     5821 ctttgaataa gccagtagaa aatgttttcc taattttgct tcgagtgaaa caaattataa
     5881 acattaacaa ataattataa ttgagcaaac ttgacttatg ttttgtaagg attatgctat
     5941 actctgggaa gaggtgagta tgatggagct aacacagaca gaattcgttt tatgccctaa
     6001 atttgaaaaa acattctcta ttttaggaaa aaaatggaac ggattgatta tcgatgtact
     6061 actcgaaaac ggtccgcaac gctttgttga gcttgcggca aaaatcccga tggtcagtga
     6121 tcgagtatta gtcgaacgct tgaaggaatt agaaaaagaa ggaatagtca ctcgaaactt
     6181 tgcctgtagc gaaaagagcc gttcagaata tctcttgact gaaaaaggag cagacttgca
     6241 aaaagcgatg cgtcaaatac aagtttggag cgaaaaatgg atcacagagg aagaatgcgg
     6301 ttgattttgt aaaaaaaatc ttgtaaacta tagtccatac gaataagtaa agaacgatga
     6361 cgaaaaagag tagtttggct aaaccagttc agggagatct gtcgcagact gaaagcagat
     6421 cctgtgtttg aaaaatgaag ttcactttcc gagttcgtct gataaaatca gaccggcaat
     6481 tgtcgttaac gaagtaagag tgcggtgtaa gtcatttatc cgcaaattag gatggtaacg
     6541 cgaagacctc gttccttaac aggaatgagg tctttttgtt ttgtatttgg aatttaaagg
     6601 aggaacaagc acatgtctct acaagcacaa ttagagagct tgaaagaagc aacgctcgtc
     6661 aaaatcacag cggcacaaga tttaaatgaa ttaaatcaaa tacgtgttga aacgttagga
     6721 aaaaaaggac cgatcacaga agtattacgt gggatgaaag atctaagtcc agaagagcga
     6781 ccaaaagtcg gaagtttcgc aaatgagatc cgagatcttt tgacagaaga gatcgagcga
     6841 aaaaaacaag tattagaaga agaagcgtta aatgcagctt tggcaaaaga aacaatagac
     6901 gtgacactac ctggtagaaa agtaaaacat ggtacacgtc atatcgtgac acaaatcatg
     6961 gaagaaattg aagatatttt tgtgggtatg ggctatcaga tcgtagaagg atatgaagta
     7021 gaatctgatc attacaattt cgaacggatg aatctgccaa aagatcatcc agcaagggac
     7081 atgcaagata ctttctatat ttcagacgag atattgattc ggacacatac ttcaccagtc
     7141 caagcacgga caatggaaaa acatgatttc tctaagggtg ctctacgaat gatctcgcca
     7201 gggaaagtct tccgcagaga tacagatgat gcgacccaca gtcatcagtt ccatcaaatt
     7261 gaagggctag ttgttgataa aaacatcaca atgggcgacc ttaaaggaac attagaagtc
     7321 gtaatgaaaa aaatgtttgg ggaagatcgt gaaattcgtt tgcgtccaag ttatttccca
     7381 tttacggaac catcagtaga agtagatgtc agctgtttca aatgtggtgg tgccggttgt
     7441 aacgtatgta aatacactgg atggatcgaa attttaggag ctggcatggt acacccgaat
     7501 gtgttgaaga tgtcaggaat cgatccagaa gaatattcag gctttgcttt cggccttgga
     7561 ccagaccgcg tagctatgtt gcggtatgga gtgaatgata tccgtaattt ctatcaaaat
     7621 gacttacgtt tcttaagtca gttcaagggg gagtaagaaa atgttagttt catataaatg
     7681 gctcaatcgt tatgtagatt tatcgaatgt aacaccaaaa gaattagcgg ataagatgtc
     7741 tgtgacagga atcgaagtag agggagtaac ggttccagaa gaaggcttga aaaaaatcgt
     7801 tgtcggtgaa gtcaaggaat gcgtaccaca tccagattct gatcaccttt ctatttgtca
     7861 agtcgatatt ggcgaagacg aactgtctca aatcgtttgt ggagcgccta atgtgaaggt
     7921 tggaatcaaa gtgattgttg cacttcctaa ctcacgtatc gctggtaatg taaaaatcaa
     7981 aaaaggaaaa atgcgtggcc aagtatcaaa tggcatgatt tgctcattac aagaaattgg
     8041 ctattcagat agtgttatac cgaaagaata cgctgaaggt atctattata tgccacaaga
     8101 agctgtaaat ggcgatccag tttttcctta tttggatatg gatgattcga ttattgaatt
     8161 atctatcaca ccgaatcgtg cggatgcatt gtcaatgcgt ggcgtagctt atgaagttgg
     8221 ggcaatttat cgtcagactc ctacattcga tgacgaaaag ctggtagaat cctcacgtcc
     8281 tgctagcgat aaaattcaag tggaagttga agataaagaa gcagttcctg cttatcaaat
     8341 cagaatcatt gaaggagtaa gaatccagcc aagtcctcaa tggctgcaaa atcttttgat
     8401 gaacgaaggc attcgtccaa tcaacaatgt agtagatgtg acgaactata ttttgctttt
     8461 attcggacaa ccactacatg catttgacta cgacaaattg aaatcaaaag agatctttgt
     8521 ccgccgtgca aaagaaaatg aaacattagt aacactagat ggagaatctc gggaattgac
     8581 aactgacaat ctcgtcatta ctaacggcaa agaaccagtt gctttagctg gtgtcatggg
     8641 agggttagac tcagagatca cgaatgaaac aacaacagtt gcactagaat ctgctttatt
     8701 tgatccaatt tcgattcgtc gtacagcaaa acaatttaat ttgcgtagtg aatcctctgc
     8761 acgttttgaa aaaggaatca accatgcgac tgttggagaa gccagtgagg ttgctgcagc
     8821 aatgatcgtt tctcttgcgg gtggggaagt tctacaaggc gcggtcaaag gctcagagat
     8881 caaagcagaa gacgtatcag tgaagatttc acttgaacgc atcaatcaat atttaggaac
     8941 atctcttgtc gttaaagaag taaatgagat ttttgaagca cttggattta gctatgaaga
     9001 aaaggagtat gtttattccg tagtagtacc accaagacgc tgggatatcc aaatcgaagc
     9061 agatattatc gaagaagttg ctcgtattta tggatacgac cagttgccat ctacacttcc
     9121 aagtggagaa acggttgctg gaagcttgtc aaaagaacaa gaaactactc gtaaaattcg
     9181 tacaatcttt gaaggaagcg gattgagcga agcaatcagt tatgcattga caacagaaga
     9241 aaaatcaaga cagtttgtta taaatgaaag taatattaca cgtttggact ggccaatgag
     9301 cgaagaacgt tctgtattac gaatgaattt gatcagcgga ctattagatg atatcagtta
     9361 taacactgcc cgaaaaaata cagagattgc tttatatgaa attggtcggg tgttcttcca
     9421 agaaaatgat ccacgtacac atctgccgca agaagtaaaa catgcagcaa tcgctatttc
     9481 tggtatttgg gaagaaaaag attggcagac gaaagctcaa aatgtagact ttttcaccat
     9541 caagggtctt ttagaaaact tatttgaaca actaggcatc actgaagatg tccattatca
     9601 aacagtgaca gaaatgaagg aaatgcatcc tggaagaact gctgcgattt accttggcga
     9661 aaaatttatt ggttttgtcg gtcaagtgca tccaacgatt acaaaaagtt atgatattcc
     9721 agaaacatat gttgctgagt ttgatttatc tgctatagtg gaagctgctc aaaaagggat
     9781 cgttttcgaa gcagtgacaa aattcccagc agtcagccgg gacatcgctt tgcttgtaaa
     9841 agaaactgtg acaaatcagg aattaacaga agttatccgt tctgctgccg gaagattttt
     9901 aacagatatc caattatttg atgtctatca aggagaaaac attgaaaaag gacataaatc
     9961 catggcgtat agcctgacat tcgtcaaccc agaagcaaca ttgacagatg aagaaatcaa
    10021 taaagcaatg gaaaaagtcg aaaaagcgtt ggtagaaaac ctagaggcaa gtattcgcta
    10081 agaaggaaaa tatagtaagt aacgatcaag tagcttttga agaccataca tttgaaaaaa
    10141 agatgtgaca caacttcgtc acatctcttt ttttgatttt aaaaaaggaa tagcacagag
    10201 acgagataaa aaaacaggcc tttacagcct gcttttttag agtaaatgaa ttaatggaag
    10261 taattgattc ccattgctga ttttacttca gaaagtgttt gtgcagctac tttagcagct
    10321 tcttcactac ctttacgtaa gatttccata acagctgaag gatctttttc caattcttct
    10381 cggcgcgcac ggatcggtgc aaattcagct tccaatacat caatgaggta tcgtttgatc
    10441 tttacatcac caagtccgcc gcggcggtaa tgagctttca tttcttcaat tgcttcttta
    10501 tctgttccga atacatccaa gtaggtgaag accatattcc cttctacttg tccaggatct
    10561 tgtacatgga tgtggtttgg atcagtatac atactcatga cttttttctg taagacatcg
    10621 gcaggatcgg agatataaat accgtttcct aacgatttac tcattttgcc atttccatcg
    10681 attccaggta atctacccat tccttttggt gggaatacgc cttttggttc caccaagact
    10741 tcaccatatg tatgattgaa gctttgtacg atttcctgtg tctgctctag cattggtttt
    10801 tgatcctcac caacaggtac aagattcgct ttgaatgcag tgatatctgc tgcttgagaa
    10861 acaggataga taaagaatcc tgtaggcacg ctttcaccga attttttttg ttcgatttct
    10921 gttttgacag tcggattgcg ccgaacgcgt cctacgctga ctaagttcaa ataatacatg
    10981 gttaattcag ctaattccgg aatttgtgat tggataaata aagtagattt tgcaggatct
    11041 aatcccacgg ctaaatagtc taaagcaact tgcaagacat ttgatgaaac cttctcagga
    11101 tttttagcat tatctgtcag tgcttgcatg tcagcaatca tgacaaacag ttggttgtct
    11161 tcatctgcct gcatagccac tcgggttttt agagaaccga cgtaatgccc taaatgcagc
    11221 ttcccagttg gtcgatctcc cgttaaaata atatttttca aaaaagacac ctctttacga
    11281 tttgtaagtc tgagcactta atatcttacc gtttttcctg aaaagaaaca acagttccta
    11341 aggaaaaaac aaggaaagag gataaaataa ctaatagatt agaaatggaa gaaatattta
    11401 tgggagaata aacgttctaa gaaactttac aaattactaa actatcagat ataatgtatt
    11461 ttattataca tctttaaaag aaatcttact aaataataat gggagtgtaa ttctatggca
    11521 atgattaaat ttgatcacgt ggataaatat tatggcaagt tccacgcatt aaaaaatatc
    11581 aatctcgaat tcgagaaagg agaagttgtc gttgtcattg gtccttctgg ttcaggaaag
    11641 agcacgatgc ttcgctgtat caatggatta gagacgatct cttcaggtaa gctgctgatt
    11701 aatgatactg acttgcatga taaaaagaca aaactgacag aagtccgaaa aaacatcggc
    11761 atggttttcc aacatttcaa tttgtaccca aataaaactg ttctggaaaa catcaaatta
    11821 gcaccaatca aagtgttgaa gcaagaccag gcaactgcag tcaaaaacgc ggagaaattt
    11881 ttgaaaactg tcaatatgtt agataagaaa gattcttatc cttccatgtt atccggggga
    11941 caacagcaac gggtggcaat cgccagaggt ttagcgatga atcctgagat gctgctgttt
    12001 gatgaaccta cttctgcttt ggatccggaa atgattggtg atgtattaga cgttatgaaa
    12061 aaattggctc atgacggtat gtcaatgatt gtcgtgacac atgaaatggg ctttgccaaa
    12121 gaagtggcgg atcgtgttat cttcatggct gatggagaag tattggaaga cagcagaaat
    12181 gtgagagact ttttcgaaaa tccaaatgaa gtccgagcac agcaatttat cagtaaagtt
    12241 atcaatcatt gataggagga aaaagatgcg caggttaaaa aaaatacgcc tgattttcct
    12301 tttaggcatt gcttttcttg cactagccgg atgtaaagga aatgggttgg caaatgaaga
    12361 tattttagag cgaagcaaat ctaccaatga aatcatctgg ggtgtcaagt atgatactcg
    12421 attatttgga atgatggata ttgagagcag aactgtacaa ggatttgatg tagatattgc
    12481 aaaagcaatc actaaaaaga tccttggtga caatggaaaa acagaatttg ttgaagtcac
    12541 ttctaaaact cggattccat tattgaaaaa cggaaatatc gatgcaatta ttgcaacgat
    12601 gacgatcaca gatgaacgaa aaaaacaggt tgacttttca gatgtctatt tcgatgctgg
    12661 ccaagctttg ttggtaaaga aaggaagcca aatcaaaagt gtagatgatt tgaatgcttc
    12721 tacaacggtt ctagcagtca aaggttctac atcggctgcg aatatccgac agcatgcacc
    12781 agatgcaaaa attttagaat tagaaaacta tgcagaagca tttactgctt tacaatctgg
    12841 tcagggagac gcgatgacta cggataatgc gattttgtta gggattgcag atgaaaatcc
    12901 agagtatgag ttagttggtg gaacgtttac taatgagcct tacgggattg cgatcaacaa
    12961 agggcaagaa aacttcttga aagctgttaa ccaagcgcta gaggaaatgc atgcagatgg
    13021 cacatacgat aaaatctatc aaaaatggtt tccaaatgaa actgaaggaa aagtagagta
    13081 agggggagag gcaatgagcg aattgataca aacttatgga ccaatctttg tcgacgggtt
    13141 caagatcaca ttgttttcga gtgtcttagc acttgtgttt agtttgatta tcggaacgtt
    13201 aatggcaatc ttccaattat caaagaataa agtagtgagc gccttagcaa aggcatatgt
    13261 agaatttttt agaaacattc cattattaat catcgtgatg tttttttatg ttgtcgtacc
    13321 gatttattgg tttaatctag atggattcca agcaggtacg attggattaa cgatctatac
    13381 atctgctttt attgctgaaa cagtacgttc agggattcaa accgtaccaa aaggtcaaac
    13441 agaagcagga ctatcttctg gttttaccta ttcagaaatc atgcgttata ttgttttacc
    13501 gcaagcattc aaaatcgtta tcccgccatt aggcaaccaa tttatcaacc tcgtaaaaaa
    13561 ctcatctatt ttagcaatcg tagctggttt agatttgatg tatcaaggag acttgatcgc
    13621 tagtgaaaca ttcaatacct tcgatacgta tatcatcgtt gggttatttt acttagttat
    13681 cacgctaccg ttatcttact taatggttta tcttgagaaa aaatgggcgg ttcgttcata
    13741 gaaggagggg aaatacatgg attttagcgg cgctttttca tggatgaata tccgtttttt
    13801 attggaagga ttaaaggtta ctgttgaagt ttcattgttt tcgatcattt ttagtttctt
    13861 gatcggtggt ctgacaggtg tgattcgttt tgcgaatatc cctttctttt ccaaaatcct
    13921 tggtgtagtg attgatatta tacggaatct tccattatta ttgatcattt tcttcactta
    13981 ctttgcctta cctcaaatcg gcattcagat gaacatcttc tggtccgctg tcgcagcatt
    14041 aacgattttt gagtcagcta tgttgtcgga aattttccga gctggactca atgctgttcc
    14101 aaaaggacag atggaagcag gattatcaac gggactgact tatgtagaat cgatgcgaac
    14161 gatcgtgtta ccgcaagcat ttaaatcaat gatcccggca attgtgagcc aattgatttc
    14221 actgatcaaa gatacttcat tagcagtaat catttcttta cctgaacttt ctcatcaggc
    14281 acgtattatt tatggacaga acaccaatta tgtgcttccg atgttcatca tcatgacact
    14341 tatgtacttc gtggtatgtt acgcgctttc tctgctttcc ggttacttgg aaaagagacg
    14401 gtacagttat taatagatga ttcttttagc ggttgtggca aactaaagtc acaaccgctt
    14461 ttcctctttc cattcgaaaa aaagagattt cgattgtttt aagcaagtaa gagcacttga
    14521 aaattcacgc taaaaagtac acaataggga ggatagaatt tattgaaaaa aaggatgata
    14581 cgattgacag ataatcgccc tatcggattt attgattcag gtgtcggcgg cttgactgta
    14641 gtaaaagaag ccctgaaaca attaccgaat gaaaatattt tatttgtagg agacacagca
    14701 cgctgcccat atggccctag acccgcggaa caggtaatcc agtatacttg ggaaatgacg
    14761 gattatctgg tggagcaagg aatcaagatg ctggtgatcg cctgcaatac cgcaactgcg
    14821 gtggctttag aagaaatcaa agctgctctt tctattccag tcatcggtgt gatccttccc
    14881 ggtactagag cggcagtaaa aaaaacacaa aataaacaag ttggcattat cggtacgatt
    14941 ggtacggtaa aaagtcaagc ttatgaaaaa gcactgaaag agaaagtacc agaattgact
    15001 gtgacaagtc ttgcttgtcc caaatttgtt tcagttgtcg aaagtaatga ataccattca
    15061 tcggtggcga aaaaaattgt ggcagaaaca ttagctcctt taaccactaa aaaaatcgat
    15121 acattgattt tgggatgcac ccattatcca ttattacgcc ccatcattca aaatgtaatg
    15181 ggagaaaatg ttcaactgat cgattctgga gcagaaacag taggtgaagt atctatgctg
    15241 ttagattatt tcaatttgag caattcaccg caaaatggtc ggacattatg ccagttttat
    15301 acaactggct ctgccaaact tttcgaggaa atagctgaag actggcttgg aatcggacac
    15361 ttaaatgtag aacatatcga attgggagga aaataaggat gcgtcatgac gggagaaaag
    15421 cccaagagct tcgcaaactg gagcttcaaa caaatgtttt caaatatcca gaaggatcgg
    15481 tagtgatccg ttttggtgat acaacagtta tttgttctgc aacaatagaa aattctgttc
    15541 cccctttttt gagagaaaca ggaacaggat gggtgacagc agaatatagt atgctgcctc
    15601 gagcaacgag tacgagaaat cgtagagaaa gcagcaaagg gaaactttct ggtagaacaa
    15661 tggaaatcca gcgtcttatt ggtcgttcct tgcgtgcagt agtagacttg gaaaaattag
    15721 gagaacgtag tatcatcgta gactgcgatg tgatccaagc ggatggcggt actagaacag
    15781 caagtatcac tggggcattt gtggcattga aattagccat tgaaaaactt ttaagagaaa
    15841 aagaattgtc agaagatcca ataaaagaac atcttgctgc agttagcgta ggaatcttac
    15901 cagatgggac ttgcgtcaca gatttggatt accaagaaga ttccgcagca cttgtagaca
    15961 tgaatcttgt aatgacagaa tctggaaaat tcgtggagat tcaaggaact ggagaagaag
    16021 caacgtttga tggtgaacag ttgaatgaaa tgctcttttt tggaaaaaac gcgattgaag
    16081 atttgatcaa agagcaaaaa catgctttgc taacagagtt tgctcaaaat gatgagcgga
    16141 tagaagaaac aaaaacaatc atcattgcga caagaaatcc aggaaaagca gaagagtttc
    16201 gaaatatgtt caaagaagcg ggatatcatg taaaaacatt acttgactat cctgaattgc
    16261 cagatgtgga agaaactggc agcacatttg aagaaaatgc ccgattaaaa gcagaaacga
    16321 ttgcacaatt gcttgatcag ccagtcttag cagatgattc aggtttgaaa gtggatgctt
    16381 taggcggtat gccaggaatc tattctgcac gttttgcagg ggaacaaaaa agtgatgcgg
    16441 ggaataacgc caaattatta tatgaattga cagatgtgcc ggatgaaaaa agaacagcac
    16501 aattccattg tacgttagta ttcgcggcac ccaaaaaaga cagccttgta gtagaagcgg
    16561 aatggcccgg tcgagtagcc cgtataccat ctggcgaaaa tggctttggg tatgatcctt
    16621 tatttatccc agaaggtaag aaacaaacag cagcagaatt atcgagcgaa gaaaaaaaca
    16681 agatcagtca tcgggctcaa gcaatgaaaa aattgagtgc cgagtggaaa caatggctgg
    16741 aaggagagag atagatgcgg tatttagtag taagtgataa tcatggagac agagatatcc
    16801 tcgttgactt agtagatcgt tatcgttctg aagtggatat ctttttccat tgtggtgatt
    16861 cggaattaga agcttctgac ccattatggg atacattcca agtggttcaa ggtaattgtg
    16921 attatggacc aggattcgaa cagaaaaaag tgatccaaac tggtcaggat acagtattca
    16981 tgactcacgg tcatttatcg aatgtccgat ttggtttgac acagttagca atcgaagctc
    17041 gagctgcaac tgccgatatg gtgttttttg gtcataccca tcaaataggc tgtgagatgg
    17101 atcagaaaat cctgtttttg aatcctggta gcatttctca gcctagaggc ccgatccaaa
    17161 tcccatcata tgctcttatc gaaagtttag atgaccaaat ttctgttcaa tactataacc
    17221 gtgcccatca ggcagttgaa gagttggcct tttctttttc gagagagcgc tgaataaaaa
    17281 aactctaatt tttaataaaa ataagcttta ttttatgaat tttgcatccg gtttcggtaa
    17341 aatagtagtg ttgaatgaaa tagaaatgga ggccatcaga tgattggacc tatagtaaga
    17401 gaaatgctgc tggaaaatca ggaaacgttt ttgattcctg cagaaaatgt ggcaaatgtt
    17461 atgtatcatc atccattgtc ccatggttta cttgtgttat cgaaggtcgg ttattccaaa
    17521 attcctgtat taggaaaaga tgatcacttt gtaggactag taagtttatc agatgtggtc
    17581 aataaaatga ttgacttgca aacaatcagc atggacccgt tagaaggact aacggttgct
    17641 gatgtgacag aaacagatgt tccgacaatt gatgaaaatt gggaactaga agaagtactc
    17701 catttgttag tcgattcgcc ttttttaccg gttgtcacag aagatcggat tttcaaagga
    17761 atcattacaa gaaaagagct gcttaaagcc gtaaacttca tggtgcatga attagaacgt
    17821 cgtaatatat tgtctccgaa aatcgaagaa ttaaaagaac aaatcgatat cgtgagctaa
    17881 agaacgagaa actaaaagat aatcaaaaaa taagtagaaa aaacagcgta ccagttttga
    17941 ctttatcttg aaaaagaggc tgggacaaaa gcgtttaact ccaagaaata agaaggaatt
    18001 cacgaaaatt gcttttcaaa tttttgtgaa tttcagctta tttccgaagg agttgcttct
    18061 gcttccgccg tttatacgtg tttagagcgt gggacaaaag tgttctttac ttttgtccca
    18121 cgctcttttt agtgagattc gttgcatcgt ttgtccaaat tattttccga cagcaattgg
    18181 actgttcgga atcgtgaaac cagaagctgt caattctttg acataggagc tcaagaactc
    18241 ttcttttaag gcatattgtt ttccgttcaa tgcgtagcag accgtacgaa tcgcaaaatt
    18301 gctgtttcca agatccacta atccaaagat agaaggttca gtttggagct cttcttggta
    18361 tttttcagct aatgtacgat ttactcgatc aatgatctca tagattttgt catatccttc
    18421 ttcaggaaca atccgaatat ctaaaagtac ttgcatattt gcacgagaga gattgctgat
    18481 cgtggtgatg tttcgattcg ggatgaaatg gacagtacca tccgttgctt tcagctgaag
    18541 ggtacggata ccaacagata cgacggttcc ttcaatcgtt aagtttgcta gcttgatgta
    18601 gtccccaaca tccatctgtt gttccatgat aataaaaaat ccggtaataa catcactcat
    18661 gaacccttgg gcaccaagac cgattgccac acctgcaatt ccggcaccag ccaaaagtga
    18721 tccgatcggc acaccgatag cagataaaat tgcatagata aaaaagaaac caagggtata
    18781 atgaaaaata gtatttaaaa gactatgtaa agtctttaag cggctctcgt ttagaatggc
    18841 ttttttagaa tactgattaa aagacttatc aatcagatat tttcccacac ggtgaagaat
    18901 ccaaaaaagg acgatcaaaa aaatcagata taaacttttt tggataaagg cagccaaaat
    18961 ggcgtcccaa tcaatcccgt cccaaaaccg ctgaaacgca tttactttgc tgacagcttg
    19021 atctgtcaat gtctcgccag aagagtctgt cgtaccggca gttgctaata atgaaaacat
    19081 aaaatcctct tttctaaaat ttgattcttt ctgtattgta ccaagaagtt ttcaaaaaag
    19141 gaaa
//
LOCUS       DACEZN010000043        18987 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEZN010000043 DACEZN010000000
VERSION     DACEZN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.043"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(16..726)
                     /locus_tag="IXH60_001147"
     CDS             complement(16..726)
                     /locus_tag="IXH60_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1990100.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(716..1576)
                     /locus_tag="IXH60_001148"
     CDS             complement(716..1576)
                     /locus_tag="IXH60_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1990101.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(1581..2219)
                     /locus_tag="IXH60_001149"
     CDS             complement(1581..2219)
                     /locus_tag="IXH60_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1990102.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(2234..2533)
                     /locus_tag="IXH60_001150"
     CDS             complement(2234..2533)
                     /locus_tag="IXH60_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1990103.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(2576..4060)
                     /locus_tag="IXH60_001151"
     CDS             complement(2576..4060)
                     /locus_tag="IXH60_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1990104.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(4080..4541)
                     /locus_tag="IXH60_001152"
     CDS             complement(4080..4541)
                     /locus_tag="IXH60_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1990105.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(4559..5626)
                     /gene="ulaG"
                     /locus_tag="IXH60_001153"
     CDS             complement(4559..5626)
                     /gene="ulaG"
                     /locus_tag="IXH60_001153"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1990106.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            5844..6614
                     /locus_tag="IXH60_001154"
     CDS             5844..6614
                     /locus_tag="IXH60_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1990107.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(6887..7714)
                     /locus_tag="IXH60_001155"
     CDS             complement(6887..7714)
                     /locus_tag="IXH60_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1990108.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            8282..9517
                     /locus_tag="IXH60_001156"
     CDS             8282..9517
                     /locus_tag="IXH60_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1990109.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(10516..10776)
                     /locus_tag="IXH60_001157"
     CDS             complement(10516..10776)
                     /locus_tag="IXH60_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1990110.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(10803..12104)
                     /locus_tag="IXH60_001158"
     CDS             complement(10803..12104)
                     /locus_tag="IXH60_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1990111.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(12396..12854)
                     /locus_tag="IXH60_001159"
     CDS             complement(12396..12854)
                     /locus_tag="IXH60_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1990112.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(12867..14192)
                     /locus_tag="IXH60_001160"
     CDS             complement(12867..14192)
                     /locus_tag="IXH60_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1990113.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(14209..15621)
                     /locus_tag="IXH60_001161"
     CDS             complement(14209..15621)
                     /locus_tag="IXH60_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1990114.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(15638..16402)
                     /locus_tag="IXH60_001162"
     CDS             complement(15638..16402)
                     /locus_tag="IXH60_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1990115.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(16506..17381)
                     /locus_tag="IXH60_001163"
     CDS             complement(16506..17381)
                     /locus_tag="IXH60_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1990116.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(17636..17720)
                     /locus_tag="IXH60_001164"
     tRNA            complement(17636..17720)
                     /locus_tag="IXH60_001164"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17684..17686),aa:Leu,seq:caa)
     gene            complement(17738..17809)
                     /locus_tag="IXH60_001165"
     tRNA            complement(17738..17809)
                     /locus_tag="IXH60_001165"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17775..17777),aa:Gln,seq:ttg)
     gene            complement(17819..17891)
                     /locus_tag="IXH60_001166"
     tRNA            complement(17819..17891)
                     /locus_tag="IXH60_001166"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17856..17858),aa:His,seq:gtg)
     gene            complement(17953..18037)
                     /locus_tag="IXH60_001167"
     tRNA            complement(17953..18037)
                     /locus_tag="IXH60_001167"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18001..18003),aa:Leu,seq:caa)
     gene            complement(18058..18128)
                     /locus_tag="IXH60_001168"
     tRNA            complement(18058..18128)
                     /locus_tag="IXH60_001168"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18094..18096),aa:Cys,seq:gca)
     gene            complement(18174..18245)
                     /locus_tag="IXH60_001169"
     tRNA            complement(18174..18245)
                     /locus_tag="IXH60_001169"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18211..18213),aa:Gln,seq:ttg)
     gene            complement(18257..18329)
                     /locus_tag="IXH60_001170"
     tRNA            complement(18257..18329)
                     /locus_tag="IXH60_001170"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18294..18296),aa:His,seq:gtg)
     gene            complement(18332..18405)
                     /locus_tag="IXH60_001171"
     tRNA            complement(18332..18405)
                     /locus_tag="IXH60_001171"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18371..18373),aa:Trp,seq:cca)
     gene            complement(18412..18492)
                     /locus_tag="IXH60_001172"
     tRNA            complement(18412..18492)
                     /locus_tag="IXH60_001172"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18456..18458),aa:Tyr,seq:gta)
     gene            complement(18494..18569)
                     /locus_tag="IXH60_001173"
     tRNA            complement(18494..18569)
                     /locus_tag="IXH60_001173"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18534..18536),aa:Phe,seq:gaa)
     gene            complement(18605..18677)
                     /locus_tag="IXH60_001174"
     tRNA            complement(18605..18677)
                     /locus_tag="IXH60_001174"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18642..18644),aa:Asp,seq:gtc)
     gene            complement(18687..18759)
                     /locus_tag="IXH60_001175"
     tRNA            complement(18687..18759)
                     /locus_tag="IXH60_001175"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18724..18726),aa:Val,seq:tac)
     gene            complement(18775..18846)
                     /locus_tag="IXH60_001176"
     tRNA            complement(18775..18846)
                     /locus_tag="IXH60_001176"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18811..18813),aa:Glu,seq:ttc)
     gene            complement(18861..18950)
                     /locus_tag="IXH60_001177"
     tRNA            complement(18861..18950)
                     /locus_tag="IXH60_001177"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18914..18916),aa:Ser,seq:gga)
BASE COUNT         5931 a         4053 c         3035 g         5968 t
ORIGIN      
        1 cttaccctag catctctatt gcccgtagta tgcatacttt ccatgtttgc gaaaataatg
       61 tttatctagt aaatattgcg ggatttcaca tatattgggg ttgatcagct cgttttcttt
      121 ggccatcaga caaatttcat ctaagatcaa actgttttct actgctttca taggtgaatc
      181 cccccatgtg aacggtccat gtccatagac aagtactcca gggacttcgt ttggatctaa
      241 ttttctttcc ttaaacgtct cgacaatcac attccctgta tgaacttcgt aagcttctct
      301 tacttcttct ttcgttaatt ggcgtgtaca aggaacctta ccataaaaag catcagcatg
      361 agtcgtgcca taagctggca aatcgcgtcc tgcttgcgcc cacatgacgg agtgcgtgga
      421 atgagtatga gtaatagcag ttatatcttc aaaagtttga tacaaaacaa catgtgtggg
      481 gagatctgaa gatggtttta agctatcttc ttctattata tttcccgaca aatctgttac
      541 caccatttga tctgcttgca tgcattcata tctgacaccg cttggcttaa tgacgatgac
      601 tcctagctca cggtccactt cactcacatt gccccaagtc aatttgacta atccgtatcg
      661 cggcaatgcc agatttgctt caaggacacg ttgcttcatt gctgcaattt tttcttcaca
      721 agacatcata gccagcctcc ttcaatttag aacttaagta gtcttttgcc tgctggattt
      781 ctcttcgaaa gtcttggctt ttttcactcc acatttcaat cagaaatgtt ccatcataat
      841 ctagacgttt caaatgtctt aataatccaa gaaaatcaac acaaccgcta ccaaaaggaa
      901 catcacgaaa ctggcctgaa tattcttttg ttacagggta agtatctttt aagtggatag
      961 cagtgatata atcgatccct ttctcaagtt ctcgagccgg attattttcc ggccatgcgg
     1021 acaaattgcc taggtctgga tatacttgta gaaaaggtga atgaatctgt tctttgattt
     1081 ctaaaaattt tgaaatggag ttcataaatg gatcatccat gatttccacg gaaagaatga
     1141 tgccatattt agaagcttcc tttacgcttt cttttattcc ttcgataaag tcttctctgg
     1201 aattcattga tttctcttca taataaacat cgtatccagc tatttggatt actttgacgc
     1261 ttagattatg cgccaaatgg attgcttttt tcattagttg tttcgctgct tttttctttt
     1321 ctggatcagc agaaccaaac ggaaatcgcc gatgcgcact taaacaaatc gaatggatac
     1381 gtacaccgga gctgaacatc tcttctctca gctcagctat cttttcttca gaccaatcta
     1441 atcgtgctaa ccgctcatcc gtctcatcaa tcgacatctc gataaaatcg aagtccattt
     1501 cttttacaag ggaaaaacgt tctttccatg aaatatcttt gggtaacgct ttttcataaa
     1561 tcccaatttg agccatttcc ttacccccag atacgaatga tctcatcttt gaagttttgt
     1621 gcggccttca tcggatcatt tgctgctgta ataccacgac cagtgataaa ggtatacaca
     1681 tcgatccctt caaataagtg taatgtgtgt ggatttaatc caccagttac agaaacatta
     1741 aagcctagtt caatcagttt tttgatcttc gataaatctt tttcacccca agtttcacca
     1801 gataacagcg catcacggct ttgatggtag attacctgcc ggataccgat gttatgccaa
     1861 tccatggctt gttcaaaagt ccaattgccg tagagttcta cttgcaattc acctatttct
     1921 ttttgtgctg cctccatagt tggaatcgtt gcacaacaaa tgactgtcat ccagtctgct
     1981 cctgcttttg caacattact cgccactgtt cctccagcgt ctgcacattt cgtatcagcg
     2041 actagttttt tatctgggaa catactgcga atacagcgaa tcgctttttg tccttcctgt
     2101 aagcaaagaa ttgtgcccac ttcaataata tctactacat caccaacttt cataacatct
     2161 cctaatgcat gttctaagtc attgtgatct aacgcaattt gcaaattcgg cctgctcatc
     2221 ttagttcctt cctttacttg tttttttggt accactttgt ttcagcaaat ttttcactga
     2281 tttctttgac tgacatcaca tttttgatac cgataactgt aactcctttt ttctctgcct
     2341 gagtaaacat attcaaaaaa ttgactggcg taaaaactac atcgtattgg ccagctgaac
     2401 tttttccctc tgaaatggca caatgatgga ttttggtaat tggtacgttt agttctttca
     2461 ttgctttttc cacacttctc atcatcatca agcttgttcc tgatccattt gcacacgata
     2521 ctaaaattct catcttaatc cctccattat gagtgttgtc taatcagatt ttcttttata
     2581 ctctctgctt ttctgcatat gtgtcatagt cttctacaat caaaaaataa ccatcaggat
     2641 ttttccgata ttgtaactgc ggaatagcca ataaaccaat gacaactacg gcaattccag
     2701 cataacctag gactttcatg attactgtaa acaatggcca agctgttgcc cagtcgaaca
     2761 tcccgatata accaccgtat gttgacatcc caatccatgt agcgaataat gctgaaccgc
     2821 ctacttgtat gattcctgaa agaaacggga acaggcaagc ggctttgaat ccacctcggt
     2881 tattggcata aactgctata acggcattat caaagaacaa cggaatgaaa ccggctatga
     2941 cgatcgttgg tgatttcaat aaaatcagta aaccaatcat caaaaactgt cccaaagcac
     3001 caaacaagaa cccaatcgtt accgcatttg gcgaaccaaa gccaaaagta gcagctacgt
     3061 caattcctgg aacagctcct ggtaaaatcg tgtttgagat tccttggaaa gattgagtta
     3121 gttcatcgac gaatgtccga acacctaatt gcaagatcgc cagatatacc gcaaaattta
     3181 aagaagtcgt cataatgtag aataagaaac tctgcccttc ttgcatgaat tgcgcttgaa
     3241 tcaaataatc tttgcctagt acaatcagaa tgatcccaaa gaaaaatagc atgagtaacg
     3301 aagtagcaac catattctca ttgaaaatcg acaagaatcc tggtaatttg atatcttcaa
     3361 tttttttgtc ttgcttgatt gatctatttt tcttttcatc ccgctttttc atccactcag
     3421 ctaattttgc aaatataaag attccgaaca tttgctgatg tgccactgca aatccggcac
     3481 cttctgtcaa ttcctgcgtg attcctactg ttaagttaga agctactgtc caataacaac
     3541 ctaaaatcag tcccataaaa atcaatgctt gtatcctacc tagatcagga aagcaaaaca
     3601 gtaaaatcca aaaagcggta gctgcttgtt gaacctgaac gtttccggta gtgaaaacgg
     3661 aacgcagttt cgtatatttc tgaaaacgga ctaataagat atttaaaaca aatgcaatca
     3721 gtaataaaat catcacatct ccaaaggtac ggccaaatgt ctccattaat ccagcatcca
     3781 cagcattttg tccaaaataa ggatctgtaa ccatggcatc caaattgaac cgttccttta
     3841 gtccaaccaa gattggccga aaattattga ccaaaccacc agagccaaca gtcaaaataa
     3901 agtaaccgac cgttgccttt aagaatccag cgatgcattc atgaagcggg cgtttcagca
     3961 aaatatagcc taataacacg ataaaaccaa tcaggtatgc tggctgcgtc aagatatttg
     4021 ttgcaaaata cgtccaaatg cttaacaaaa attcccccat cttttttctc ctctatcaat
     4081 taattatatt ctgccatcac aacttggtaa tcatcgatgg agttcgtaga gatgagacgc
     4141 tcaaccaatc catctgtcat taataattcg gataattttt ggatattttc tagatgttct
     4201 tctggatctt ttgctgcgag tgtaaaaaac aaactagccg ttttctcctc attctcccct
     4261 tcactttcaa aacggattgg ctgtttcaac ttggtaaatg aaatagctgt tccaaaaaca
     4321 ccatcacttt tttctgaaga atgcggcatt gctacttgag gtgcgatcac aatataagga
     4381 ccgtattttt ctacacaatg gatgatctcc tccacatacg tttcatcaat aatttcctta
     4441 tcgatcaata caagacagct ttcggcgata gcttcacgcc aatccgtgat ttctttatct
     4501 gggtaatgaa tcaattgatg gtcaaaaaag tatttcagca agaaactccc cttctttctt
     4561 ataaaaacga cggaaacggt gtatcatttt caatcgagaa gacatcttca aatcctcgat
     4621 aaaaattaaa ctcaagatca tctttgtttg tcggatagat gaatttcccg cctacttgcc
     4681 agatataagg tttaaatcca tacttcaggc gatccttttt gaatttccaa atttcagtga
     4741 tctcttttgg atcagccata aaattagacc aaatatcgta gtggacagga atcactactt
     4801 ttgcattcaa tgactccgcc atgcgcagca tatcaacaga agtaactttg tctgttatcc
     4861 ctcgtgggtt ttcaccatag gcccctaaac agacatcgat tttgtgttca ttaccatgtt
     4921 tggcaaataa attggagtaa tgtgaatctc ctgcatgata aagattccct cctgatgttt
     4981 cgaataaata gtttactgca atttgatcca tctcctgagg catttttcct tttaatacct
     5041 cttctggatc ttcacaagtg accaacgctg tccgatcaaa cgcttctaaa gctactacat
     5101 tgatgtcttt gattttgact gcatcacctg gctttactgt aatcgttttt tcttcaggaa
     5161 ttccccattt ttgccacgtg cgaaccactt cttttggccc aatgaatgtt gcttcaggac
     5221 aattttgatg aactgctgcg gctgtatgga tatctagatg atcggaatgg atatgtgtaa
     5281 cgattaaagc gtctacattt tttatcgcaa aaggatcgat tacgaaaggc tgagtacgta
     5341 aattaggctg catattttga acgccactca tacgcatcat ctgatggcct tttttcatca
     5401 tgccattccc atgactacgt tttcccgtcc cacaccatag atcacataaa atattcgttc
     5461 cttcatgact tttcagccaa attcccgtac agcccagcca ccacatagca aaagttccag
     5521 gatccactac ttctgtttcg atttcttcat tcaagtatgt gccccactca ggaaatgttt
     5581 ctactaacca cttttctttt gttacttcat tgacagtttt actcatttgc ttgctccccc
     5641 catatcgttg ttccaactta caagtaaata ttaatgtgta tcagcatcca tgaaaaccct
     5701 tttatatgac cgtttgtttt ttatatgatt ttctttcagt aatcaatgat tgtttcagta
     5761 taataaagac agatatgaat gtttaaatga acaactagtc aaataaatcc atggatcaag
     5821 atgtccctca ggaggcaaaa gtcatgaaaa actcacttgt caatattgaa aaacgtcaaa
     5881 aagaaatttt ggaactgctt cagcagactg aacaactatc aactttcgaa ttagctgaat
     5941 cccttgatgt atctattagt acgatacgaa gagatttaaa tttattagaa gaaaaaaacg
     6001 agatcatacg caattacggt tattgttctt ataattacaa aaaccagact gattttgatc
     6061 aatctggtcc tgaaaggatt aaacaagcga ttgccagaga agctagtaaa tacattaacg
     6121 attatgatac gctgtttatc aattctagct cgactgcgtt gaaaacactg gattttcttc
     6181 aatctaacca tttgacagtc attacaaaca acgtgaaaat caattactct ccgcacaaag
     6241 ataattgtag ttatatttta actggtggag agatgcgctt tccaaaagaa gcactagtgg
     6301 gcgacatcgc attaaatacg atttcttcag tcaatgcaga catttgtatc attggctgta
     6361 gcggagtcga tgtagtacat ggggtcacaa caaagatatt aaatgaatca acgatcaacg
     6421 aaatgatggt caacaagaca atcaaatgta agattttagt cgctgatcat cgaaaaatcg
     6481 gactgacatc aaagttcaag attgctgata tttcagtctt tgattatttg atcactgata
     6541 aattttgttc tgaaaaagta ttagaggaga tcagaaatac ggggataaaa accattcaag
     6601 tagattcatt ttaagaacca ggacgtaaat atgtaaatag gattatcaaa aaaatgaata
     6661 acaaatttat cgataaatga aaaacacgct acgctcaaat tgataaagga atcccaagat
     6721 gtatccatcc aatcgatacc atcataatct attgcttctt ttataaccgt tatatgttgc
     6781 ctaaatagga atcaaacaat atcgaaaaaa ggaggtatga caaaagtcac acctcctttt
     6841 ttcgaataaa cggtattcaa atcagaacac tgatttcaca accactttat ttcaggtcgt
     6901 tttcaatcac ctttaccgcg taatcatttc cgttgaaacg cgctattttt tttgcgtcca
     6961 tatatctttc taaataatca tcttctccta attgcttatg gcaagaagca atttcataaa
     7021 gcaagtattc caatgcataa gtcgatttat gtttcttatt atactcaata cccttctcac
     7081 ataattggat tgctttatca taatcctcga ttttacggta ataggtggcg atatcataat
     7141 atacaatgca gaccatcaga ttatcaaaat cagtctcact caatatttta agtgtacgct
     7201 ccacataaac tttcgcaaaa tctaattctc cttttgacaa gtagatcatt gccacgtttg
     7261 cagctgcttg taagtgatgg atatctgttt cggtcgtttg ttccaacact tgattaaaat
     7321 aaaataatgc tgcatctggt tttttcgcat aaagatgatc aagcagtcct tgataaaata
     7381 aaatttgatt taaaagatca gaattactta ctttttcctg atcgatcgtt tgtaaaattt
     7441 tataagattc ttggtatttt gatacagaat tcaatgattg aatctcattt atttttttcc
     7501 tgattttgtc ttcttctgtt tctactaata tatcggagat ctcgacattc agctttttgc
     7561 aaagcgcact taagatattg atgcctggcg acatattctt gttttcgata ttgctgacag
     7621 ttgcttgttc acaaatgcct tctgctaatt gtacttgtgt taaccccttt ttctttcggt
     7681 attcttttag taatgctcca tctactttaa tcaaaacaat ttccttccct ttaaaaaatt
     7741 atttctcctt tatatcatac acaaaaaaaa gttctaaaca accaaaaatg tttagaacag
     7801 aataaaaatt ttaattttaa aataatattc aaattaaaca actggcgtat ctgctccttg
     7861 atttcttttt gtgttatctg aataattatc gtttacagtt ataacacata gtgaaaattg
     7921 tcatggcgag aaagtctaag aatattttta aacataacta tttctttctt acgtgtgtat
     7981 aatgacgaca gcacacctat caattaatat aattatttta tttttttatg ttctcatgtt
     8041 tatttttaat gacacaactt ctcgtattta tctggcacaa catataaatc gatcaaaaaa
     8101 ttgttgcgtg tttttttgaa aattggagtt tcaacttgat tacaaaattt aaatttagaa
     8161 gttgtgaaaa cagcgagaga cagtttaata ttcaaaatat acataggcta ctcgaacgct
     8221 gtgtttttta gtatactagt aagaaacgaa gggatagact cattccaata aggaggattt
     8281 catgaagaca attctttttg ctcctgcagt attcaattta gctgagacaa cacgaatgat
     8341 tgaggttgcc aaagctatga aagaccgtta ttactgtgta ttctttggct tttctaaaac
     8401 ttttgctcct ttgatcacag aaagtggctt tgagtatcat ttactagcgc cgatccttac
     8461 gaaaaaacaa gaagaacaaa tcataaaatt tgaccagttg cgtacactta aaaacccttt
     8521 taccaaagaa atggttgcct ctcgtatcac tagtgaacag agtctgattg ctaagtttga
     8581 gcctcgactg attgtgacag gttcgaatgt gacaattttt ttatcggccc gcaataaaaa
     8641 gattccttta gtatatgtta aaccctatgc tttgagtgaa cctttttttc aatcagagca
     8701 tgcgctgatg ccccacgagt tacgtttctt cggcccttta agcaatcccc tttggcactg
     8761 gacaaaaaag cgtatagtaa atacccgatg gatccccaat gccttcaaac acgctgcaca
     8821 aaaagaaatg cttcctttac caaaaacaag tttacaaatg atggatgctg atctaaatct
     8881 catcacaacg cctaaactat ttttggaaga agaacagctg ccggaaaatt atcgaatcgt
     8941 tggtccaatt tttgctaagc tcagtacttc cttgcctgaa gaagtgctaa cttttataca
     9001 gcgaaagcaa caagaaagaa aacgtattat ctatttcgca atgggtagtt caggcaatcc
     9061 gcgtatgtta cgcaaaattt tgttcttctt acaaaaacaa ccggatctag cggttattgc
     9121 acctattgac tatctgcttg atgacattca atctgactca gaagatcttt taataacaga
     9181 atatctgtct agtcatcttt tgcatgaatt cattgatttt tcttttatcc acggcgggga
     9241 agggaccgtc caaactgcct gtgaatccgg caaaccgttt atcggtgtgg ggatgcaatt
     9301 tgagcaaagc tgtaatattg actattgtgt aaaatacgga aacgcaatcg ccctcacaaa
     9361 accagtgact gaaagaaaac tgcagattgc tctgcgcgaa gtttcttcac ccaagattcg
     9421 tcagcaggca cgcacgctgc aagccgtttt tccaataaat ggtccacaac aagcaattca
     9481 agaaatcgaa accttcctga gcaaccaccc aatatagttt caagtagaaa aagctttttt
     9541 aatagagact catcagtaat ttcgctattc gagacccaga cagtgaaaat acaaaaaaag
     9601 taaatagtgg attgaaaagt tagagttact taaattacgg tgtctaaaaa acacaaaatc
     9661 atccttaaaa taaaagatct gacgctatcc aaatgagcat aggtagcgtc tgccatttat
     9721 tcttagtcaa attctttgac acctttctct ggttggatca tctcaacgtt tcttcaaaag
     9781 aaagaccata ataattaggc aaatacattt tcttaaaatg tgtcatatag atctagaagc
     9841 atcgctttgt agagatttgt gccgctagtc ccctcaacaa ttactaaatc aaatgtagga
     9901 tttaccactt tatctttgat aatcaacctc cctcttctgt tgagacaatg atctcgcaat
     9961 ccgatgcata ctcctatata aactgccagc aatccccaca tattgagtag atcatgtcag
    10021 aatgttctat gtcgtcagca acgatacact aaaagtcacg ttcaccatga ctgattgcta
    10081 ttcccagcac aattatttct atacacgttt catgatttga ataaacactc acatcataca
    10141 tattaccatt catacaccat actgcagccc ctactatatg gttgtaagct cccaaatcta
    10201 tatttgactt gatcactttg gtagtagatc tactaattct aaatccattg ctgataaaat
    10261 ttccattttt tcctccttct ttctgccaaa acagttaaaa caataataat ctttatgtaa
    10321 aaaagggctg tgacaaaagc cttgtcacag tcccatattc cgaataaacg gtggtcaaaa
    10381 gcttgcatct gcggtaataa gttcaaccaa acgaaaaata agccaacatt tcacaaaaat
    10441 ttaagaagca attttcgtaa atttcttctt atttcttgaa gctaaccact tctgttacaa
    10501 ccacttttag aacatttaaa gcttcgatgt gacaaaccat ctatgctgct cgatcataaa
    10561 attgatagca aaaattccac aaagtacata tgtcccccat gaaaacaaaa cattcatatg
    10621 cggtacaatg atctttggaa gaatcacgat caaaaatgat gccagtgccc aaatccatcg
    10681 attgatttga atatgatatg tagctagcat attcaatata aggtgactag ccgttaataa
    10741 aaagataaaa acaaaaaata aaatcagcca atacattgtt tcccaccttc taaaaaccaa
    10801 acttagacag tctggacttc gttttctcca cgactttgtt catctgacag catcttttta
    10861 tcataagctt ttacaaatgg ataatagata ataaatgcta tcaatgtgtt gatcatgatc
    10921 aagataacaa cacgccagtc atttcctgct gaaagataac cgccgattgg agctggtaat
    10981 gtccatgttg gcaaaacagt aaatctattg accatttcta atttgactgc aaaataactg
    11041 ataatagtca aaactaaagg aactaagttg aacgggatga acatgtaagg attcaacatc
    11101 ataggagctc caaaaattat gggctcattg atattgaaga taccaggaat aatagaaaaa
    11161 cgaccaacct ctttcaagaa ttttgattta cagaagaata taaacaaaac agataccacg
    11221 atcgttgctc ctgcaccacc aattgttacc gtccattgat aaaactgttc aggagcgata
    11281 tagggtaatt gggtggcagc tgtaccatct gccaatgcct ttgcattctc atccaacatg
    11341 atcagccaca ttggacgaac aacagcccct acaatactca tgccatggat accaaatgac
    11401 cacaacaaat ggatcaaaaa cattggtagt aaaacaccaa gcaaattatt ccctaaaata
    11461 ttggttaaag gctcgaataa cgataataag gcagcgttta catcaaagcc cgcgacgctt
    11521 cgaatcagcc aagtaattgt taaaatgact gcacctggaa tcaatgcctc aaaactacgg
    11581 gcaacagcag gtggcacttg ttctggcatt ttgatagtaa tatttcgatt tttaaagaaa
    11641 cgatatactt ccactgcaaa aatcatagat aagatccccg caaacatccc agatgctccc
    11701 aaagaactca taggtaagac ccagccaaga ggtgcctctt caggagttaa aacgtttaca
    11761 ggaacttgta acataaagaa cgttcctaaa gataatatac cacctgtcac actatctaat
    11821 ccatatgatt tagctagatc cgaacccata acaaaagttg cataaatagc catcaatccc
    11881 atcgtcatcc taaagggtaa aacaatcgtt gccgaataag gggcaataaa ttcattccat
    11941 ccaggaaacg gtaaattgat aaagaccata aaaaatgtcc caatcaaaat caatgacaat
    12001 gttgaaacaa ccccattacg aattgctaat aaatgacgct gtgtaccaat ttttgccatt
    12061 ggtgggacaa gtttctcttc gacccatttc atcagctttt ccactaaaac cactcctttt
    12121 caataaaata tgaaccattc attgcatgac aacgcaaaga atgttcataa ctaattatat
    12181 aattaagaaa ttttatttca atatattctc ttaaataatc aaatttattt ttaagaatcc
    12241 tgaaaataag ttttctaaat cataggagca acaaaaaaat tcgataatta acaaaaatca
    12301 aaatgattaa atctagtgat tgaaacatat catttcaaaa aaattttgta aattcaccca
    12361 taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca actatcttta
    12421 atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg agaaactaca
    12481 atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat agctaacagt
    12541 gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact tccttcgagt
    12601 tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact ttcgatcgtt
    12661 atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc atggatatat
    12721 ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa gacctcgtct
    12781 tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat gtttcttgtt
    12841 ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt tttgtttact
    12901 ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt ttgattcaat
    12961 gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac tagtaacttc
    13021 tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg ttttcttagt
    13081 gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac tgcatctgac
    13141 cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg acgatcatca
    13201 ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa attaaagttc
    13261 aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt atttttctct
    13321 cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc aacgaccata
    13381 tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa atttttcgtg
    13441 atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt aaaatcggcg
    13501 acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc tcgttgcaat
    13561 tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata cgtaacgatc
    13621 caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga aatagtcata
    13681 tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc agaaacgatc
    13741 gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg tatatgatcg
    13801 acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc gagtccacac
    13861 gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc tcgcatctcc
    13921 aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga
    13981 tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat aaatttaaca
    14041 atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat ttcagataat
    14101 ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata cgtactcccg
    14161 cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct attcttctaa
    14221 taatttattg aattctgcca taacataagt cacttctaag ccgatgataa tttgataatt
    14281 tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct gttcctcgac
    14341 catggtggga tccactacct ctacccgtag gcgtgtatta caactataga catccaggat
    14401 attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat aatttttact
    14461 tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg ccttagcttc
    14521 acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa
    14581 ggcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga ttcgaaaatt
    14641 gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat tgaataagta
    14701 acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca taggagaaag
    14761 aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag ttcctgcacc
    14821 gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt tatagatagc
    14881 tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata aaaacagtcc
    14941 agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt ccttgctagg
    15001 agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt gcagtccaaa
    15061 ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc ccatatcaac
    15121 gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa agcccaaaaa
    15181 aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt cgcccgcaaa
    15241 ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc atgccgctac
    15301 taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca taatttctaa
    15361 gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt
    15421 atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt ttccaaaaac
    15481 ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg ataaacgcat
    15541 gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc caagcttctg
    15601 cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca tatgttacta
    15661 actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga
    15721 cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga tccaaatacg
    15781 ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt aaacttgcca
    15841 aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct cctacgacat
    15901 cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca aagttgctcc
    15961 gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt tcatccaaag
    16021 tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga taaatttttt
    16081 gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat ggatgctgat
    16141 aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt ttgaggagtg
    16201 gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt tcctttctta
    16261 gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat gtcaaaattc
    16321 ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca aaatcatccg
    16381 aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa taggcgcaac
    16441 caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat agttacgccc
    16501 ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt ttcttgaatg
    16561 gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca atacgctttg
    16621 ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc ttcctgataa
    16681 atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt cagtgattct
    16741 ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt cgtcatttct
    16801 actattttta agctctttaa actattaggc tctcctagct cagcattaat gatatgcata
    16861 gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt gattcgctcg
    16921 accacttcta ctgctatatc atattcattt ggataaagga atctcatttc tggcaacata
    16981 attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat atgatctgtc
    17041 aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat cagttcaatc
    17101 acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc actgaatcgt
    17161 ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac atcaaatggc
    17221 ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc tattccatcg
    17281 tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt taattcacct
    17341 cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt tgtctccaaa
    17401 caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg tgcctaattg
    17461 aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa gcgttatcaa
    17521 tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag gtaattacat
    17581 aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat aattataccg
    17641 gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc cagcgcgtct
    17701 gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg attcgaacca
    17761 acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa gaataaaggg
    17821 cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt taaccacttc
    17881 gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact aagatgaaga
    17941 catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg gcactggatt
    18001 ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt agtactaagg
    18061 cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta ccacttggct
    18121 ataccgccat tatactatta tcaatctata aaataaagaa taaattatag aaactggggt
    18181 agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata
    18241 ccccaagaat aaaaagggcg aatgatggga atcgaaccca cgaatgccgg agccacaatc
    18301 cggtgcgtta accacttcgc cacattcgcc atggcagggg cagcaggaat tgaacccaca
    18361 ctaacggttt tggagaccgc tgttctacct ttaaactatg cccctataaa atggaggaga
    18421 gtggattcga accactgaac cctaaggaac ggatttacag tccgtcgcgt ttagccactt
    18481 cgctactcct ccatggtggc gcgggacaga atcgaactgc cgacacacgg agcttcaatc
    18541 cgttgctcta ccaactgagc taccgcgcca tattttaaaa ttattaattg aataacaaga
    18601 acgacggttc cgacgggaat cgaacccgcg atctcctgcg tgacaggcag gcatgttaac
    18661 ccctacacca cggaaccagt tcattatgga ggttaacggg atcgaaccgc tgaccctctg
    18721 cttgtaaggc agatgctctc ccagctgagc taaacctcca acattcaatt aaaatgaccc
    18781 gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa
    18841 cgggccaggt aaatttgcta cggagagtaa gggattcgaa cccttgagac agtttggcac
    18901 catctacatg atttccaatc atgctccttc ggcctctcgg acaactctcc agagtgaaag
    18961 aagctcaata ctctttctca aaatgag
//
LOCUS       DACEZN010000044        18325 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEZN010000044 DACEZN010000000
VERSION     DACEZN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.044"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            128..442
                     /locus_tag="IXH60_001178"
     CDS             128..442
                     /locus_tag="IXH60_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1990117.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            420..2114
                     /locus_tag="IXH60_001179"
     CDS             420..2114
                     /locus_tag="IXH60_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1990118.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2134..3312
                     /locus_tag="IXH60_001180"
     CDS             2134..3312
                     /locus_tag="IXH60_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1990119.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            3275..3961
                     /locus_tag="IXH60_001181"
     CDS             3275..3961
                     /locus_tag="IXH60_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1990120.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            3961..5121
                     /locus_tag="IXH60_001182"
     CDS             3961..5121
                     /locus_tag="IXH60_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1990121.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            5131..6006
                     /locus_tag="IXH60_001183"
     CDS             5131..6006
                     /locus_tag="IXH60_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990122.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            6003..6314
                     /locus_tag="IXH60_001184"
     CDS             6003..6314
                     /locus_tag="IXH60_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990123.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            6304..6657
                     /locus_tag="IXH60_001185"
     CDS             6304..6657
                     /locus_tag="IXH60_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1990124.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            6647..7048
                     /locus_tag="IXH60_001186"
     CDS             6647..7048
                     /locus_tag="IXH60_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990125.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            7041..7445
                     /locus_tag="IXH60_001187"
     CDS             7041..7445
                     /locus_tag="IXH60_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990126.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            7457..8062
                     /locus_tag="IXH60_001188"
     CDS             7457..8062
                     /locus_tag="IXH60_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1990127.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            8082..8444
                     /locus_tag="IXH60_001189"
     CDS             8082..8444
                     /locus_tag="IXH60_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990128.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            8447..8629
                     /locus_tag="IXH60_001190"
     CDS             8447..8629
                     /locus_tag="IXH60_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990129.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            8646..12065
                     /locus_tag="IXH60_001191"
     CDS             8646..12065
                     /locus_tag="IXH60_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1990130.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            12116..12853
                     /locus_tag="IXH60_001192"
     CDS             12116..12853
                     /locus_tag="IXH60_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1990131.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            12863..15154
                     /locus_tag="IXH60_001193"
     CDS             12863..15154
                     /locus_tag="IXH60_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990132.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            15178..17268
                     /locus_tag="IXH60_001194"
     CDS             15178..17268
                     /locus_tag="IXH60_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990133.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            17431..17877
                     /locus_tag="IXH60_001195"
     CDS             17431..17877
                     /locus_tag="IXH60_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990134.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            17879..18016
                     /locus_tag="IXH60_001196"
     CDS             17879..18016
                     /locus_tag="IXH60_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1990135.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            18054..>18325
                     /locus_tag="IXH60_001197"
     CDS             18054..>18325
                     /locus_tag="IXH60_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990136.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT         6503 a         2751 c         3830 g         5241 t
ORIGIN      
        1 agctttcatt ccgatggtga gataaccccc ccttaaaata aatcgcaaat tttttggggg
       61 tgatgaaacg gagggggctg tcaggaaaag agattttttc gaactttatc atgaaaggag
      121 ggctaaaatg tttaaaaacg aattgtctca aaatcgctac agagaaaaat tacgccgctc
      181 tttaataagc caattggaaa gtcagaaaac aaatattgag ccattcttag ataatgttga
      241 tcgttatatc agtttatggg aaacggcgat atcactggaa gaagatatat ccgagaacgg
      301 cattagactg gagaatggta aaaagaatga atcagtagcg ttgcttgttt ctgtcaacaa
      361 acaaatggga ttgatgttgg ataaacttgc cattactcct gaattggtag gtgaagcaaa
      421 tgaatcaatt cctgagttat aagcatattg aaaattggtt caaagctata gaagaaggca
      481 ctatcaaggt atgcaaagag caattattgc taaaaaatta tctagaagaa agagtcttta
      541 ctagagaaga tatttacttc gataagcaga tggtagagga ttcaatcaat ataccagcac
      601 aatactttcc attcgaatta attccgtggg aaaaatttct acaatgtttt atttatggtg
      661 ttcgatggaa aaaagataaa acactagtgt tcaatagata tctttcatta atgggacgtg
      721 gtaatggtaa aactggtttt gcttcttgga acaacttctt tctactaacc gctaaacacg
      781 gtattaaaaa ttatgatatt gatatctatg ccaataatga aagccaagca aagactagtt
      841 ttgatgatgt atttaaagta attaaagatc atcctgattt agataaaaaa gtatttaaag
      901 ctacgaagga agttattcaa aatatcgcta caaacagcaa acttcgttat aacacggcaa
      961 atgctagaac aaaagatggg aagcgaccag gtgcaaaccg ctttgatgaa attcacgaaa
     1021 atgaagatta ttcaatgata aatgtggcta cttctggtgg tggtaaaatt cgagattata
     1081 gagaatttta tgatacaact aatggtcatg ttcgtggtgg tccgcttgat gacattatag
     1141 aagaatcaaa aatgattctt tctggagaac ttggaattga caaggatgga gcagaatttt
     1201 ctagtttgtt tccatttatt tgtcgcttgg ataacgataa tgaagttgat gatcccgaca
     1261 tgtgggaaaa agcttgtcca actattaatt acaatgcaga tttaaaacgg aaaatgtttc
     1321 aagaatactc tcaaatgcaa cgtaatgctg gtttaagact tacgttcatg accaaacgaa
     1381 tgaacagacc tatggaagat acacgatttg ctgttgcttc atatgatgat gttctgcata
     1441 cgaaagaaaa agaatttcct gaaaaaatgg atgaagtgat aggaacagtc gattttgctg
     1501 atagacgaga ttttgccagc gttgggttgc taggaaaata cgataaagat gtttatttta
     1561 cacaacatac ttttatccac gaatcagccc ttcgattaca aaacatcaaa cgagaggtta
     1621 tagatatttc tatagatcaa ggaaaatcac agatcgttca tggaaaaaat atagaagctg
     1681 attatattgt aggttggttt cttgaaatga gtaataaata ttatattaaa aaaatcgcta
     1741 tggatatgta ccgtgcaaaa atattgaagc ccgctttaga agaagcaggt tttactgtgg
     1801 aaattgttcg aagcggatct gttacacatg gtatgttaaa agatctggtt gatgaccttt
     1861 ttattaatca acgtttattt tttggtgacg atgcgattat gcgttggtat tgcatgaatg
     1921 tatatgaaga gcatatttct aatggaaata tacgctatga aaaaatagaa cctgaaacta
     1981 gaaaaacgga tggctttttt tcattccttc atggtttgaa ttttttagat gatatttatg
     2041 attctgctcc tgtaacagtc acaaatagct cagtagaaaa tacaggaact ggatttactc
     2101 ctctagtatt ctaacttgaa aggaggtgag aaagtgggga tttttcaaaa ggcggtagga
     2161 tacttcacaa aaaaagcaac ggttccttta gaagaatact tttgtaaatt gcaagttgat
     2221 tttgtgtatc gaaaatttgc gattgaaact tgtattgatt tgattgcaaa tgcgatgagt
     2281 aaagcggaat tcaagtcata tgaagatgga aaaaataaaa agaatgatct ttactatagg
     2341 ctgaatgtag ctcctaataa gaaaaataat gcaacagaat ttagaaaaaa actgatcagg
     2401 agattaatat tctacaatga agtattgatc gtttctccgt ctaataattc tagcgaaata
     2461 tttattgcgg atagttggga tgtcacagaa tatgcattga aagatgatgt gttttctcaa
     2521 gtgcaaatta acaacatagt ccttgataga gaatttctag aaagtgatgt tatctatata
     2581 aaatacgcag atcaacaaat taggcaacta gtcgatgcgt attatcaagc gtatgggaaa
     2641 ctcatttcta gtgccatgaa tgtttacaag cgctctaacg ctcgtagata cgtactgaaa
     2701 gggaatttat tccgaccgca agacaataca acacaagatc aaatcaataa aatgatgaca
     2761 tcacaattta aggcttttat ggaagctgat aatgcaggtg cggtatttca attacaaaat
     2821 gagtacacat tagaagattt cagcggaaac ttccaaagca attcaagaga tataaaaaac
     2881 ttaatagacg acatctttga gatgacagca gcagcgtttc acgttccgaa aaacctacta
     2941 aagggagaca tgagtgggtt atcggatcaa gtggacgctt ttttaatgtt cgaaatcata
     3001 ccgattgctg aacttattca ggatgcgttt aacgctagtc tctatgaagt agaagaatac
     3061 ttgtcaggga attttgtacg tgtggataca actatgatca agattactag cttcaaagat
     3121 ttggttgacg ctattgatgt aggcattaga aatggagtat ttacaatcaa cgaaggaaga
     3181 gagcgcgttg gaaatgatcg ctctgataag gcgatggcag atgaaatatt tataactaaa
     3241 aacaatcaac aagtatcgaa aggaggtgag gcgaatgacg acaatgaaaa catttctagc
     3301 agtaaagaat gaaggcgcag taccgcaaat ttttattcag ggatttattg gttctagttg
     3361 gttctttgaa gggaatactg acaagggaat caaaaatatt ttggatagtc taggtgatca
     3421 agaagaaatt gaagtagtaa ttaattcaaa cggtggagac gtatttcaag ggattgctat
     3481 tgggaactta cttaagtcaa ataaagcaaa agttaacgtt gtgattaacg gcttagccgc
     3541 tagtgctgct tcaattatcg caatggctgg cgatactata aaaatctaca acaatgcaca
     3601 attgatgatt caccgcgctt ccacatacgg agaaggaaat gtcgatgact tccgtacgat
     3661 tgctgaccaa ctggaatcaa ttgataaatc ggtaaaggct tcatataaaa cacgattcaa
     3721 tggcacagat gaagcattgc aagaacttct tgaaaaagaa tcgtttatgg atgcagaaac
     3781 agctttgagt tatggattgg tcgatgaaat tatcgatgca gaaaatagct caggtactga
     3841 agctaaaaaa gaacaaagcg ttgaagaaat tttgaatgac gttaaagaaa aaagagcaga
     3901 aaaaattgct gcatttacag cagcattaaa taaaacattt ggacaaggag atgtaaaata
     3961 atgacagtta aaaatttaaa aggtgtaaca gctgcaagcg accaattgat gaaagctttt
     4021 aaagatggta acgaagaatc ttttagcgca gctatggtaa gcttatctaa ggaaattcag
     4081 gataaaattt tagaagaagc aacagcaaaa aatcaagatc aattagtatt aatgaaccgt
     4141 ggtcagcgtg tattaactac acaagaaaca aaattctata acgaagtagt gaaaaacgaa
     4201 ggttttgcag gggtcgaaga attagtgcca gctactgtat ttgaacgtgt atttgaagat
     4261 ttagaacaat ctcatccact attgcaaaaa attacttttg ttaacacaac tggtgtaaca
     4321 gaatggattg tgtcacgtgg agtcaatcca gcatggtggg gtaaactttg cgaagctgtt
     4381 aaaaaagttt tagataatgg ctttgatgta attaacatga agcagttcaa gctatcaggt
     4441 tatattcctg tatgtaaggc aatgcttgat ttaggtccag tatggttaga tcgttatgtc
     4501 cgtactgttt tagtagaatc attgagaatt gcattagaac aagcaattgt tgatggtact
     4561 ggtaaagata tgccagtcgg aatgatgcgt gacatgagca aacaaactag cggagaatat
     4621 gctgaaaaaa aagcagaacc tattacagct ttagatgctg taactatggg cggtttgatg
     4681 gcgcgactat caaaattcaa tatcgaaggt gtgaatgatc cgatttatcg taatgtgaat
     4741 ccttctgatg tggtcctaat tgtgaatcca actgattact ggtctaaagt attcccagct
     4801 aagactgtac taactgctaa tggagaatac gtacaagtat tgccagtacc agtttcagat
     4861 ttgcagtcaa cggctgtgcc agaaggaaaa gcagttattg gggtagcctc agattacttc
     4921 atgggtgtag gatctacact aaaaattgaa gcttcagatg aataccattt tgttgaagac
     4981 gaacgcattt atctagctaa acaatatgca aacggtcaac ctaaacgtaa cgatagtttc
     5041 attgtattag atattagcgc tttgggaact actactacaa ctacaaaacc aacaaccaca
     5101 acaactacaa cacaagcgta ggtgatcaga atgaagtata ttctttgtca gccggcaatc
     5161 aatcggttta aatgggagct tgaagtttgt ttaactaatc tgaagaaact aggaatcaaa
     5221 gatatcgtat tgcttttcag cagacacgat gatcagattc ctattttttt tgagaaggaa
     5281 tatggtgttg aagttcatgt gtacgatgat ctgcgggacg acaaagagta tattccttcg
     5341 attaaaccat atttatggtg gaaatattta gaagaagatc attcgcgtga ggacgaccga
     5401 tatttctata tcgattcgga tgtcattttc aataaaagaa ttaatttgcg caaattgcct
     5461 tctaaagatg atgtttggta ttgtagcgac tgctgtagtt atctaagtct tgattatatt
     5521 agaagctgtg aaaacggaga aaatattcta aaagatatgg caaacattgt aaatgttaca
     5581 gtagaatctt tggaaactat aaacactaat tcaggaggcg cacagtgggt tattaaccgc
     5641 cctaaagcga attattggaa aaaggtttat ctggattcta atcggctata tcgctacctt
     5701 agagggcaaa aaacaaatat acaaatctgg acagccgaga tgtgggcaca gctttggaac
     5761 atgatgtatt tcaatattgg tcctaaagtt cacgaggaat tagacttttg ttttgctact
     5821 gatccaatag aaaaagttaa agaagtaaaa atcttacaca atgctggagt aacaacaaac
     5881 gatgaagatt tatttttcaa agggagatac gtgacttcta cgccttttga tgaagattta
     5941 tcatttgtaa acaagaaaaa atgctcttac gcatatgtta aagcaattaa ggcggtggtt
     6001 agatgacgcc tgaacaagtg actgaagaat tgctaatagc tgtgaaggat aatatttacg
     6061 ttacctggaa cgaagaagat gagtcaatta aaaagatgat agctaaaaat gctgtttatc
     6121 ttcaaagtaa agtgagtaca acactttctt tttctcctga aagcttagaa tacggattgc
     6181 taatcgaaag atgtagatac gactggaatc gtgctttaga tgagtttgaa caaaatttcg
     6241 ctagtgagtt attaggtttc attcaacatt atgcgctaca agaatatatt gcaggtgatg
     6301 tgaatggcga ataatcgtag actcgaagaa acattcaacg atggttggtt aaagattttg
     6361 acgcaaacta ccaaaagaaa tgaactagga aaaaagattg gtgtagaaga tacagaaatc
     6421 acttctttaa aatttagaaa tctttccatg agagatagtg atataacagc tatggatgcg
     6481 atgggatcga aattaactaa gaaagtaaag actccatttc atccaatcgc caagaaattt
     6541 aataaagatc aatattttat cgtaatcgat agtatgcgtt acaacgttat ctatgccgat
     6601 tacgataatt tttatatcta tttttatctt gaaagtgtgg gtgaatatgg tgattgataa
     6661 ttctaaagaa aaagaacgtt taaataagca aatttctgct atcaaaactt ccttagaaga
     6721 gcattttggc ctcaaactct ttcaagactc cgtaggcgag gatgagctac ctgatgattt
     6781 taattacttc attctcgaaa caggagaaat agaaatgatc actgagccaa aatatagcgt
     6841 gggtcaaaat ctatatctaa ctttctattc agaaaataga gaagatttaa caggagattc
     6901 actagatatt atttcattga ttcaaaatcg ttcgattcgt tttcagagaa tggatcccaa
     6961 tcatttaaaa ctagagaacc aagatcgcta tatcgatcaa ttggtattta cgtttagacg
     7021 attattgaag agtgattgtc atggctaaaa atagttggga gctaaaaata aatggacatg
     7081 atgaacttct tgtgcggatg gaacgctatt caagcgagag cgaacgactg attaacgaag
     7141 cattgaaatc aaaaggttcg gctattgcag tggataggat tacagaaaaa attcctgttt
     7201 ctgaagcaga tttaagaaga gggcaccaac acgcaaaaaa tagtcgtcca cttaagactc
     7261 aatacattaa tttgggtttc atcattagac ctacaagaaa atttgagtat ttaaaatatc
     7321 ctgatttggg gataggtact tctaaaagaa atcagccgga cgaatttatg agaagaggat
     7381 taggtcttgc acttgatcca attacagaac ttctgattcg tcaattcgat aaattaaata
     7441 aataggagga acaacaatgg ctaaaacaac aacagtaaca acttttgaca atataagtat
     7501 taaaagaatt tcttttaatt ttaagaatgc aacaaatgca atttcaactg attgtaatgg
     7561 acaattagat ggcgaaacag aaatgcagag aattgtaaaa aaatgcggtt caacagaagt
     7621 aaaatcgaaa tctaaaccaa tcaatatgac ggtaacaatt actgcacatg taccgatgga
     7681 agtttatcga cgcttcaatg ggttgaaaca agatgaacgt attaaatcgg gaatttactc
     7741 ttatggtcct gattccgtag gggaagattt ctctcttgct gcagagatcg tggatgattt
     7801 cgaagaaaaa aataagctga ttggtatgtt agcatgcact tcgaatacag gattaacatt
     7861 ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa aagttatgca
     7921 agatgaattt ggtaaatttt atcatgaagc aattgttgca gaacttgaag aagacttaac
     7981 agatcaatgg atgacgaatc tatctgctga tgtgattaaa aaaacttcag ttgtgacaac
     8041 tacggccact caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa gattactcaa
     8101 aaattgaact aaacgatgga acaattttga atttagaacc taaactgaat atcaagaaat
     8161 tattgatgat caatagagat tttaacacag acgagtttgc aaaaatgact gtgggaaaag
     8221 gatccatgga tatttctgtt attcaaggtg caaaggctgt gtatattgct taccgccaag
     8281 cgaacatgac tgattatatt tcattcgatg aatttatcga taaatgggat tttgatatgg
     8341 ctactgccag ctatatttat caattgatga tgttcaaaca agcacgcgat gcttatcaaa
     8401 aagaatttga aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc caaagctctt
     8461 agttgaaacg tgggtcgatg tctattcgat gttgaccgac gttttttcta tgccttcaga
     8521 tttggtttta agcgatatct gtttagatga cattttgcaa atggcttaca acaagagtgc
     8581 ttatgaagga tggaaaaact atgcaataaa ccaatcccag aaaaactaaa gaaaggaggt
     8641 aaaaaatggc taaaaagaga acagaagcag aagtaacatt catagctaac gatgacggat
     8701 tgaaatctac gttaaaagaa atcagtgctg aattaactaa aaatagagca gaattaaaac
     8761 tagaacaagc tcaattacaa cagactggtt ctgaatcaga caagttagga agtaaattat
     8821 cttctttaga aaagcagtat gaattacaaa gtcaaaaagt tgaagtaact agccaacgtt
     8881 tagccaatgc caaaaaatat tatggagaaa attccaccga agttcagaaa cttgagagag
     8941 aactgattaa ccaacaaaca gcgcaacaac gtttgtcaaa cgaaattgat aaaacgagta
     9001 atgcactagc tcaagcaaaa ggcgaaatac agacgtacga gtctacaatg caacagttgg
     9061 atagtgaaca aaaaaatgtt caagctagtg cttctctgat tgaatccgaa tacaaaaaat
     9121 ggcaagcaac tgctggtcaa tcagcttctg aatccgagaa attagcgaaa gcccaagaat
     9181 atgtttctca acaatctgaa aatgcagaga aaacgataga tatcctgaga cgacagttag
     9241 aagctacaca gtctgagttt ggcgctacat ccacagaagc aatgcagatg gaggcgaagc
     9301 ttaatgatgc tgaacgtgaa tttgaagagt taggacaagc tgctaaaaat gtagatacaa
     9361 ctaacttgga cgatatcgga agcaaaatag atatgaataa tctaatggaa gcttctgacg
     9421 ttttaagcga cattggcgat aagcttacag aattagggaa acaagcagtg gactctgcta
     9481 atagtgtagg tagttcccag agtaaaatac aagctaattt tggtttgact aaacaagagg
     9541 ctgaagaatt aacgaatgta gccagagaca tttattataa aggttttgga gaatcgttag
     9601 atcagtccac agatgcattg attttggtaa agcgtaattt aggcgattta aataatcaag
     9661 atttacaaaa tatcacggaa caagctatgg tcctagaaaa caccatgggc gctgatatgg
     9721 atgaaacgtt acgtggtgta aatggcttaa tggtcaattt cggcttgagc gctcaagatg
     9781 caatggattt aatggtttcg ggtactcaaa acggtttaga taaaacgcac gaattaggcg
     9841 acaatatggc agaatatagc caattatgga gtcaaatggg atattcagct gatgaaacgt
     9901 tcggaatgct tcaaaatggt ttagatgcgg gtgcttataa ccttgataaa gtcaatgact
     9961 tagttaagga aatgggaata tcgttaacag atggtcgatt tgagcaaaac atggatatgt
    10021 ttagtgaaag tactagaaaa gcttttgaag agtggaaaaa tggcggagga acacaaaaag
    10081 acgttattaa ttccatgatt caagatttta gcaatatgga tggtcaatac gaccaattaa
    10141 ataaagcttc tacaatttgg tctgcacttg gcgaagataa cgcgatgaaa gttgtccaat
    10201 ctttgactga tgttaaccat acatttgatg atgttagtgg atctgcacaa aaaatgaatg
    10261 aagattctac tactccgtta caagaattaa acggaaaaat agctgaatta aaggattcat
    10321 tagctcctat aggcaacaca atcatagatg cactcgaacc agtaattgat tttctaggaa
    10381 agatggctga tgcgtttaat aatcttccac aaccagtaca ggattatgcc gtagcgattg
    10441 gcggattgac tgctgcattt actttattaa tgccaataat agttggcttc atggctctag
    10501 gtggtcctac tacattaata ataggagcag ttattactgt tattgctgga gttatagcaa
    10561 ttataaaaaa ctggggcgca attactgact ggtttaaggg aatatggagt aaattcactg
    10621 attggttggg tggtacttgg gaaagtataa aagaaggtgc ctcatcagtt tgggatggag
    10681 ttaaagaaac ctggtctgga tttgtagatt gggttcaaga tatttggcaa ggagtttctg
    10741 attggtttgg agagctatgg agcggattag ttgaaggagc ttccaacatc tggcaaggag
    10801 tccaagagac ttggcaagca ttcgttgatt gggtttcaaa tatttggaac ggagtcaaag
    10861 aagtatggtc gattatttgg gcagacattg taggaattgt tcaaatacca tggaccttaa
    10921 taacgtcatt gattcaagcc ggtattaata ttatcgtggg tatttttgat gtagctggac
    10981 agttattagg cgcagcttgg caagctgttt ggacacctat ttctgatttc cttaaaaaca
    11041 cttgggatac tatgacacaa tgggtaagca tcgcttggaa tggaattgta actacattcc
    11101 atactatatt tgatccagta gtggcatggt ggaatggtat atggacagct attagtacta
    11161 cggcttcaaa tatttggaat tcaattagtg caacagcttc tagtatttgg aacagtatca
    11221 agaatacaat cactagcttg gtacaagcag ctgctacagt aattcaaaat atttggtcaa
    11281 ctgtatctag ttggttaggt ggaatttgga attcaatcag ctctacagca tcaaatatct
    11341 ggaatagcgt gactagtagt ataagcaatg ctataaacgc agctaaaagt gccattcaaa
    11401 gtgtttggaa tagtatatct tcgtggatca gcggaatttg gaacggtatc aaaaacactg
    11461 ctttgaatct ttggaatgga attacaagca ctattagctc taaagtaaac gatggaaaaa
    11521 atgcaatttc aagcggttgg tccaatctaa caggtattgt ttccgacata ttcaataatg
    11581 ttaaaagtac aattgctaac atttgggaag gtatcaaaaa gactgttagc gctccaattg
    11641 attggatcag agataaaatc agtagtatct ttgataattt gaatatttct ataccacata
    11701 ttccgttacc acattttaaa ttgagcggag aattcaatcc attgaagggg aaaatcccaa
    11761 cgttgggtgt tgattggtat gcgaaaggta gtgtgtttaa ttctccgaat attatcggtg
    11821 tcggcgaagc aggacctgaa gcagttttac ctttgaaaag atctgtgctg caagaaattg
    11881 gtgatcgtat cttgagtagc acatcagttt catctagggc acaaacgatt caacctgtga
    11941 ataactacga attcaatttc acaattgatg gtaacgcaga tgaggttact atgaagcaaa
    12001 caactcaaca aatcattgat agcattacaa aagttcaaaa tgataatgct tcggcatggc
    12061 gttaaacagg agagtatttc tcctgttttt ttagtattaa aaaggatgtg aaaaaatgac
    12121 tgattgtata cattctataa tcgatggatt tcctgattat ttgcataaat tggctttagc
    12181 tgaaagacca accatacctt ctccaaaaag acagagagtt gaaacttctg ttttaggaag
    12241 gttaggtggc ttagtacaag attactcgtt tgaagacatg tcgtttacat tgcactataa
    12301 ctatttagag gatgtggaag accatcaagc gttcaagcaa tcgttttata tcatgcgtca
    12361 ttggttaaat tatgcaaaga aattagaatt ctctgatgat cccaacgtct attatgttat
    12421 ccagactatc gatattgggg atgcagaaaa cgatattgtt gaatggggag agttcgatgt
    12481 aaatattact gcgaaaccat tcgcaagagt tcaagaagat gtacctataa ccgtagataa
    12541 accacagtca tttaacttgc tgaataatag tttagaagaa agttttccaa agattatcat
    12601 cactccttca gctacttcat gccagttcat cttaaatgat tatgtgttta gttttgaagg
    12661 cttagtagta ggaactgacg tagtcattga tagtgatttg atgctttgct acgaagagca
    12721 atcggacgga gatattttag atcggtccaa caaaatgaag accatgcaat atccgacatt
    12781 gcaagtggat attaattatt ttaattgtac tggtttgagc aaaatacaaa tttatcgtaa
    12841 tgggttaagg taggtgaaat agatgatcga taatttaata actatttacg ataaaaacga
    12901 cgcgaataat ttagctgaac atttatatga tacgcaaggt ttaggcgctt tgtcagactg
    12961 gttaacagct actgttagca ataaattaaa cggagccgag atatttcagg gtacttatcc
    13021 aataagcgga actaatgcag atttgattgt agaaggacgt attattcagt gttatgtaga
    13081 tgaaaatcga gcaaaacagc gtctacggat ttattatgca aagacttctg taataggcaa
    13141 tacgatagaa gtaaaagctg aacctatttt caatgatata agaaaatcgg tgttgaataa
    13201 atatgacagt ggaacagaaa agatcactgc tagtcaggca tggcaaaacg caaaaacttt
    13261 agcgaaacca gttattcctt cacagttttc tttctcgtca ttagtagata cgcttgctaa
    13321 tgtgaagata gaaaaggcga attttttaga attctttggt ggaaaagagg gatctattct
    13381 agatcgattt catggggaat ttctaaaaga taataacaca ttacgtcatg aaaaaaggct
    13441 aggcacggat cataaaatca aagcgattta tactaaaaac ttaactggtc ttgacttaga
    13501 gattgatgct caaagtgttt tagttggagt ttatccattc attagcagct cttcagaagg
    13561 agaagacgag atcactctac cagaagaagt tattttcacg gattacgtgg atgattatcc
    13621 tgctggatat gtttcttttg ttgattttaa agacaaagcg actgatgtag ccacattaag
    13681 ggaagctgct aaagactggt tgaaaacaaa catagataaa caaaaaccac aagtgagtgg
    13741 ttcgattgaa ttagtaccat tgaggcatca aagaggctat gaaaaatttg ttgatctaga
    13801 aaaagtttcg atgggtgacg gagtagatgt gtatcatcca cagttaaaag tgaatatgtc
    13861 agcgagaatt gtggaatata cgtttaatgt tctaaccaat tcatacgata aattagttgt
    13921 aggaaacgtc aaaacaaact tcttagaaaa cacagagaat aatgtcagca atttgattaa
    13981 tgatgccatt gatcaattga aaaacggtgg cgaaatcagt gatttactca atgatattgt
    14041 agatcatcaa actgatatga ttactggtca aaatggtggt tatgttttat tagatcctaa
    14101 agaagcgcct agtcgtattt tgattatgga cacaccagat aagaataccg caaggaatgt
    14161 tttacaaatc aacaatgctg gtattggttt ttctaaaact ggcattaatg gaacatatga
    14221 aacggcatgg acgttagatg gcggattcaa tgcctcgttt attacggctg gtgaaatagt
    14281 aggaattact attagaggta ctacattaat tagtgatggt gctgattata gaacaagtat
    14341 tgctaatggc aaaatgactt ggtactcaaa aaaagttaac aaagatatta tggagctaga
    14401 agcacgtgat tatgtaagtg ctgatgccgg tattgtatca tacaccatga aaactggtgg
    14461 tggtttcatg attagaaatc cacagggtaa cttggttttt agtacgtggg ataatggtaa
    14521 taacagaccg tttctatctt ttggtgcgcc caatttcagg tatagcaatg ctagttatgt
    14581 aacttctggc gacggtagtt ctttaagcat taatggtagt gcgggtaact catgggaatt
    14641 taaggtagct ggtaggacta tgaaatttac tagtgatggt atgctaacgt taccaggttg
    14701 tttttttggt tcatgggaag atgggaaact tgctaggttt gaacaatcaa cggtacaagt
    14761 atataaagat tttactgtta gaggtactaa aaactcaact gtaccaacag aacattatgg
    14821 acaacgacta ttgaacgctt atgaaactcc agaatattat ttcgctgatt atggggaagc
    14881 cgttacaggt gacgatggta aagttcgtgt tgatattgac cccatgtttg ctgaaacagt
    14941 aaatctaagt cggtatatga cacatgtgac acctacagaa ctagttttgt gtgctgttac
    15001 tcatgaagat attgaccatt tcatcattga aactagtaag ccaaacgtat tagttagatg
    15061 gaatttagtg gcacaccgtc tagggtatga agatattaga ttaaaagagg atacagcata
    15121 tgatagcaca gtgcttgacc aaaaacgttt ttaaaacgaa gacaaggagg tatataaatg
    15181 gctagcagtt tatataattt ggctttagat ttcagcaaag aattaaacta caccaaagct
    15241 attatggctc gtcaaggtga taaagggatt acggtgacgg ttaaaccgtt tctaaatggc
    15301 ttgcagatgg atacgagtgg cggaacattt actttaaaag gaacaacacc atctaaccgt
    15361 tacgtagata atgttgcaac tagtgtaact agtgaagaag tcacgttttc tcttgatggc
    15421 acatttatga gtgaagcagg atattataaa cactgctacg tagaatatag aaaagacaat
    15481 caaattttaa caacgcaaga tatcattttt ttctcactag gagtgtctga catttcgcaa
    15541 ggccaagccg atgaatatgt ttcgcaatta gaagagttga ttcgaaagta caacgaaact
    15601 tttgatgctt ttatggctga aatcaaaggt agagtggata gcttaaatca acagattact
    15661 gatttaactg gtcaagctaa aacgctacaa gacaagttag atgctctgaa agaagaaatt
    15721 tctaagttag gtaacttaca agtgatgtac agtaacagca tcgacttcgg gggatatgat
    15781 tatagcggta agccgaattt aatgtccaaa ctaaaatcga gcgattttaa cgttggttac
    15841 cacgggtcac taactttgga taacgaaaag ttacatttta cttctgatgg tacaggaagc
    15901 attattatgt ttacgcgtat taatacacct cagcttacta gtgggaaaac ctatactctg
    15961 agtgcgaaag ttcgatttga tgaaggaact acaggagcta ttgataaatt acgtttggtg
    16021 tatcgtacat caccaggaga aaagatatta ttggaagcaa atagtacaaa tattacaaca
    16081 gatgatgtag ggaaagaaat aacaatcaaa ggtacagcta acgttaatta tcaaatcaca
    16141 aatttagatc gattttatat gagtattagc tttgttgaca gggataaaat aaatggcgga
    16201 tttaagttgt acgacatcaa aatcgaagaa ggctcaacag ccacaccgta ccagcctaat
    16261 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
    16321 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
    16381 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
    16441 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
    16501 gtagaaggct taactaatgt gtggtctaaa acatttacag cagcagatga ctcacaccct
    16561 actaccccac aagtgcagat ttatcaagta ccaagcacaa gtgtaggaca atgtacaatt
    16621 aagtggttaa aactagagga aggcaatact cgaaccccga atattagtga gtataaatat
    16681 cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt atgtttggga tctagcacca
    16741 gaatatgtcg aagataattt ggccacagat attaaaattt ctgaaattac tggtaaagca
    16801 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
    16861 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
    16921 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
    16981 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
    17041 tattttgtgg ctttaaacgg aaaacgcagt ggtggaaatg attcaacgat actaacaatt
    17101 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
    17161 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
    17221 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
    17281 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
    17341 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
    17401 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
    17461 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
    17521 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
    17581 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
    17641 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
    17701 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
    17761 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
    17821 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
    17881 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
    17941 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
    18001 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
    18061 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
    18121 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
    18181 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
    18241 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
    18301 aacacacgca acaaattaaa acttt
//
LOCUS       DACEZN010000045        17643 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEZN010000045 DACEZN010000000
VERSION     DACEZN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.045"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            6..305
                     /locus_tag="IXH60_001198"
     CDS             6..305
                     /locus_tag="IXH60_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1990137.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IXH60_001199"
     CDS             378..1739
                     /locus_tag="IXH60_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1990138.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IXH60_001200"
     CDS             1761..2021
                     /locus_tag="IXH60_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990139.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IXH60_001201"
     CDS             2035..2787
                     /locus_tag="IXH60_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1990140.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IXH60_001202"
     CDS             complement(2881..3486)
                     /locus_tag="IXH60_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1990141.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IXH60_001203"
     CDS             complement(3539..4513)
                     /locus_tag="IXH60_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1990142.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IXH60_001204"
     CDS             complement(4891..5670)
                     /locus_tag="IXH60_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1990143.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXH60_001205"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXH60_001205"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1990144.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IXH60_001206"
     CDS             7161..8558
                     /locus_tag="IXH60_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1990145.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IXH60_001207"
     CDS             8639..9079
                     /locus_tag="IXH60_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1990146.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IXH60_001208"
     CDS             9227..9553
                     /locus_tag="IXH60_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1990147.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IXH60_001209"
     CDS             9554..10303
                     /locus_tag="IXH60_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990148.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IXH60_001210"
     CDS             complement(10383..10838)
                     /locus_tag="IXH60_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1990149.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IXH60_001211"
     CDS             complement(11265..11540)
                     /locus_tag="IXH60_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990150.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IXH60_001212"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IXH60_001212"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1990151.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IXH60_001213"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IXH60_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1990152.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IXH60_001214"
     CDS             14770..17436
                     /locus_tag="IXH60_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1990153.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
BASE COUNT         5610 a         3076 c         3655 g         5302 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 ggg
//
LOCUS       DACEZN010000046        17636 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEZN010000046 DACEZN010000000
VERSION     DACEZN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.046"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..452)
                     /locus_tag="IXH60_001215"
     CDS             complement(<1..452)
                     /locus_tag="IXH60_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1990154.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRK"
     gene            complement(634..948)
                     /gene="trxA"
                     /locus_tag="IXH60_001216"
     CDS             complement(634..948)
                     /gene="trxA"
                     /locus_tag="IXH60_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1990155.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(1035..3395)
                     /locus_tag="IXH60_001217"
     CDS             complement(1035..3395)
                     /locus_tag="IXH60_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1990156.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(3510..4058)
                     /locus_tag="IXH60_001218"
     CDS             complement(3510..4058)
                     /locus_tag="IXH60_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1990157.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(4116..4553)
                     /gene="zapA"
                     /locus_tag="IXH60_001219"
     CDS             complement(4116..4553)
                     /gene="zapA"
                     /locus_tag="IXH60_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1990158.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            4755..5672
                     /locus_tag="IXH60_001220"
     CDS             4755..5672
                     /locus_tag="IXH60_001220"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1990159.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(6050..6961)
                     /locus_tag="IXH60_001221"
     CDS             complement(6050..6961)
                     /locus_tag="IXH60_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1990160.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(7362..7940)
                     /locus_tag="IXH60_001222"
     CDS             complement(7362..7940)
                     /locus_tag="IXH60_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1990161.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(7960..8628)
                     /locus_tag="IXH60_001223"
     CDS             complement(7960..8628)
                     /locus_tag="IXH60_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990162.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(8643..9713)
                     /locus_tag="IXH60_001224"
     CDS             complement(8643..9713)
                     /locus_tag="IXH60_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990163.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(9884..11248)
                     /locus_tag="IXH60_001225"
     CDS             complement(9884..11248)
                     /locus_tag="IXH60_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1990164.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(11245..12804)
                     /locus_tag="IXH60_001226"
     CDS             complement(11245..12804)
                     /locus_tag="IXH60_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1990165.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEWNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(12860..15340)
                     /locus_tag="IXH60_001227"
     CDS             complement(12860..15340)
                     /locus_tag="IXH60_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990166.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(15324..15659)
                     /locus_tag="IXH60_001228"
     CDS             complement(15324..15659)
                     /locus_tag="IXH60_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990167.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(15673..16707)
                     /locus_tag="IXH60_001229"
     CDS             complement(15673..16707)
                     /locus_tag="IXH60_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1990168.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(16797..17477)
                     /locus_tag="IXH60_001230"
     CDS             complement(16797..17477)
                     /locus_tag="IXH60_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1990169.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         5051 a         3659 c         3040 g         5886 t
ORIGIN      
        1 cattttctca gaggaaataa tcgatcgagg atcttctttg tctcattcgc cgcaccgaca
       61 tcgggatatg gtccaaaata cagtgctttg tcttttagta ccttacgagt aatcaccaaa
      121 cggggatatt tttcattcgt tatcttaagg aaaggatagg ttttatcatc ctttaacata
      181 atgttgtatt taggatcatt tttctttatc agattgatct ctaagagcaa tgcttcgata
      241 ttcgattctg taacgatgta ttcaaagtct tcgatttcac taacaagccg ttccgttttc
      301 gtattatggc taccagtaaa atatgagcgg acacgatttt ttaatatttt ggctttgccg
      361 acataaatga tcgttccatt tttatctttc atcaagtaac agccgggctg atcaggaagt
      421 aaagctaatt tgtttttgat tcgttcgttc atctggtact ccccttcata ttcaattaaa
      481 gttattatag catgtctgtc aaaaaaacaa tccagcctga caacgtaatt atactcaaaa
      541 aatattaatt tgaataacct taactcaacc gcaaaaaaac cgaggacgga actggattca
      601 gtcccaccct cagcttattt ttgctgtaag tttttataaa tattgatcga tcatttgacg
      661 tagttgttct tttgaatgaa caccaacagc tttttctact acttgaccat cttttttcaa
      721 cattaatgtt ggaatgctca tgataccgaa ttgttgagga gtttctggat tttcatccac
      781 atccatttta gcaattctga attcttcttc atcatattct tgttccaact gatccaaaat
      841 cggtgcttgc atacgacatg gaccacacca agttgcccaa aaatcaatca atacaagtcc
      901 tttatctgtt tcttcattaa atgtcttatc tgtaattact tgtgacatat caaagacctc
      961 catttttaaa atctatgact agtatagcat ttctatactt ggatagcgaa ccatctgctt
     1021 acctttagta agctttactt aaatttaaca attgtcgcac catttccgcc ttggtttgcc
     1081 ggtgcaaact caaagctttt gacgctccga tggtttttaa gataatcagt aattcccttg
     1141 cgaagtgcgc ctgttccttt tccgtggaca atagtcactt gaggataacc agctagaatg
     1201 gcagaatcca aatattgatc tacctcactt agcgcttctt cgtatcgttt tcctcgaagg
     1261 tccaattgat tcggcacgtg actactagat tcagatcgta cagcatggat aactcgttgt
     1321 gtcggctctt tttgaggagc aacaggcgtc agctcgtctt cggacacatt catttttaat
     1381 ataccgattt ctacttgcca ctgattcccg tttttacgta aaagcgtccc tctttggccg
     1441 taagtcgtaa ccaacacttc gtctcccggt ttcaacgttt tttgttcttt cgccttcttc
     1501 aatactttat ttttcttcaa agtttcttct tgatgtagat ttgctaattg ggtctttgca
     1561 tcgatcaact gatgctcttt cacatttcct tgcccaatct gctgttgcat tttacgaata
     1621 tcagcaatga ttttttctgc tttttcttcc gcctctgaca cgacttcatt tgcttttttc
     1681 tttgcttttt ccatttcttt ttcacgttct tcaaagaaat agctgtaagc ttctttcaaa
     1741 tcatcgtgaa gttcttgtgc ctcagaaaca aaatgacgca tttccagata ttcggtctct
     1801 gccattttcc ggcgattctc taaatctgtg atcatttcgt ttaaatcttg gctctcatca
     1861 ttcatcaact gcttcgcttc atcaataact tctgtatcaa gacctaatcg gctagagatt
     1921 tcaaacgcat tgcttcgtcc aggaacgcca atcagcaaac gatacgttgg acttaatgta
     1981 tccacatcaa actccatgct ggcattaatc gtatttgccc gattatatcc ataaactttc
     2041 aattcaggat aatgcgttgt tgccatcaca taagcagact ttttaccaag atcatctaag
     2101 attgcgattg ctagagcagc accttcttgc ggatctgttc ctgcaccgag ctcgtcaaat
     2161 aataccagac ttttttcatt gacttttgat aaaatatcta cagtgttcgt catatgagaa
     2221 gaaaaggtcg atagactttg ctcaatcgac tgttcgtctc ctatatccgc aaatacttcc
     2281 tcaaaaatac cgatttgact ttcttcacct gcaggaatcg gtaatcctgc ttgccccatc
     2341 agttgtaaca gacctaaagt cttcaacgtg atcgttttcc cacctgtgtt aggtcctgta
     2401 atgacgatgg cttgatagtc ttttccgatt gtgatgtcat tagaaactac tttttcctga
     2461 tcgatcagtg gatgtcttgc ttgtttcaag acgacatgat tgtcttcact gattcccggc
     2521 acgatggctt ttacttcttt accaaatcgt gcttttgcat tcatcagatc cattttacca
     2581 atcacataag tattgtggat gatttcttga cgatggggaa caagttcagc agaaagttca
     2641 cttaaaatac gttgaatctc atttcgttca gcgatttggt attgacgcaa acgattattc
     2701 aaatccacca cttgttttgg ttcaataaat aaagtctgac ctgaagcact ttgatcgtgg
     2761 actacaccgc caaatacacc gcggtattct tgtttgactg ggataacgta gcggtcattt
     2821 cgcatggtga tgatcgcatc acttaaatac tttgcatttt tcccacggac gatcccatct
     2881 aactgttcac gaacagcttg ttcacttcgc cgaatgttct gtcttatgct ctttaactca
     2941 ggagaagcgt catcagtcac gcgtccgtct tcatcgattg cttcctttaa acggcggctc
     3001 aaaacaggga tcgtgactaa ctgatctgcc cacatataca aacgtgcaaa ttcgatttca
     3061 ctgtcagaaa gatcatcaat gaaacgattc aattcgcttg tcgtagacaa tactctggaa
     3121 acttgtgcaa gctccactcc attcaagtct gctccgatct caatccgttt catatgggga
     3181 cggatatttt caattttagg tatagggatc ccgcctcgca gacgttgcac tttcaatcca
     3241 tcctcagttt cttgaagcca gtttgtgatc gtttgtttct cactcactgg taccaattct
     3301 gccagttctt cttttccttg tgcagtcact acaaattgtc tgaccatctg tttgactttt
     3361 tcaaattcca atgtttcaag gattcggtta ttcattttat caccttatct ttcactatga
     3421 aaaaagagct gtgccggaca tacgctacgc agccttctta aataaactac tctgtacaaa
     3481 aaacgaaata ttttccagca ctattttcct taattcaaaa tattcgttac ccaaagctca
     3541 tacatcttgt tggtaagaaa tggcgttctc tcaacgataa aacgagcgaa actatgctcg
     3601 ccgaattgat tttggaccac atctaatgga acaaaggcaa gtaactgcaa aatcaaaaat
     3661 atagtaacgt aagccatgat tagtgacagg atgcctcctg caatccagtc tacctgtctc
     3721 aagatcggca catacgtcaa tccatggaca aaaaccccca caaatcgggt aagcagccag
     3781 ccaattagca aaataatcag aaaagcagtt cctgcataaa aagcttcatc taggtgaaag
     3841 gcaaatacct gatcaaaaaa tgctaacttt gatgttgggg tgacagctgg gtatggaatg
     3901 tataattcca aatgacttgc taatggtttg taaaaatgct gggcagcaat aaatgacaat
     3961 acatatccaa tcgcgaatat gatttgcaaa gcaaacccgc gacgtgctcc agaaaaaaac
     4021 gcaattagca ataaaaataa aatcagcaaa ctcagcatgc tacgttcctt tctcttctaa
     4081 cttttttaaa tttttcttat tttgataaaa ggatcttaat tagaagattt ttgtttaatt
     4141 tgtccttttc gtttctcgtt taaaatctgt tgtgcttcca catgattttt aatggtatga
     4201 tctgcttgac cattttcttt caatacttcc cgagcttcgt tctcgattgc ttcaatgcgt
     4261 ttcacacgat tttcaagctc tgtaaatttg atcatttttt tctttaattc tgctgtttct
     4321 tcttccagct ccaatattct ttcttgtttc ttcaattgat ccgaaagcgc attgacagcc
     4381 atcaagattg ctgcttgttc attatccatt tgtggtgata attgtttcaa ttctgctaac
     4441 tgttcattga tcagcttcgt tacgatatcc atatgatgct tggtttcccg tccgataata
     4501 gtatatgttt ggttggcaat caccgctttg tagcgtgttt tttcgtgcgc catacgaatc
     4561 atttcctcct atttgatgaa agttttattt agtgtaccat gaagttggtt ctttcaccgt
     4621 tctccacaaa tatttttcac atacttaact attatatgat aaactttaaa aaaatgctat
     4681 gtctcgcaat ctcttagaaa acgaaaagtc gtgagttgcg gtatagtttt atgtataaaa
     4741 acgaggagaa ttttatgagc aacgtcatta tcaaagcaac aaacgaccaa cttagtcaaa
     4801 tgaaagcata ctatcgctct tatttgttaa ataaacagat tccttatact agctttgctg
     4861 caaaaaaaga cgggacgacc attaccgctt atacatccgg aaaagtgatg tttcaaggta
     4921 cgaatgcagc aatagaagct gataaatggg gggaggcaca ggataaacaa gccaagccag
     4981 caaaaacagc ctcttctttg ccaaaaaata tcagtcaatt atccgtttta ggtagtgatg
     5041 aggtcggaaa tggcagctat ttcggtccgc taacagtatg tgctgcttat gtgaaacgtg
     5101 atcagcttgc agaattacgt aaacttggtg ttagagattc aaaagagtta aaagatagcc
     5161 aaatcatcca gcttgctgac atcttaaaaa aaaccatccc ctataaactg cttgtcttat
     5221 ctccagaaaa atacaatgag atccaaccaa aatacaatgc cgttcggatg aaagtcgctt
     5281 tgcacaatca agcgatccat ttgctgctaa aaaaaatcga gccagaaaaa ccagaagcga
     5341 ttttgatcga tcagtttacg ccggaagcaa attttaaaaa atatgcgcgt ttagaaaaaa
     5401 atcaattaca agaaaaactg tacttcgtta ctaaaggaga acaatatcat ttagctgttg
     5461 ctgctgcttc tatcatcagt cgtgccagct ttttggaaga gttagacaaa gcatcagctg
     5521 aagctggaat cacccttccg tcaggagctg gaacgaaatc tgaccaagta gcggcaaaac
     5581 tattggagaa aggcggcatg cctatgcttg aaaaatatgc caaacttcat tttgccaata
     5641 cagaaaaagc tttgaagcta ctaaaaaaat aatggaactg tgacaaaagt catgtcacag
     5701 tcccttgttc cgaataaacg gtgttcaatc atctgatcct cggtattagc tcaacaaacg
     5761 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcagaaca aaacgttgat
     5821 ttcacaacct ttttcttaaa cggtgttctc atgccaactc ctcggtcaca agtcacaata
     5881 gtcatcaaac atgagaagct gttttttgaa attgctcctt gtgactcaga gttgaacggc
     5941 aggctcacga cctcttcctt aaacggtgtt taaaagcttg catctgcgat aataagcccg
     6001 gccaaacgaa aaatatgagg agctattttt gtttggctgg gcttatttat tactgaaaat
     6061 aagtggtcaa tgcttgtgtc aaacttgcag caatctgctg ctgataactg tctgttacta
     6121 attcctttaa atcactatca ttattcatat aacccatctc gattaaaata gaaggttgaa
     6181 gattttctcg aagaacttga tagttcccag tggagattcc tcttgaatcc agaggcagat
     6241 cagtgaagga gtcagcaatt gtctgtgcta atgtttcctc ttgtccatag taataatatg
     6301 tcgtgattcc agttgcttca ttcgcatatt ccgtagagtc tgcgtgtaaa ctgataaaga
     6361 catctgcctt tttttcttca ctgtagtcgc atatatctcc aagctgtact aattcatctg
     6421 tagtatgggt caaataaacc gttgctccag catcctctag cgcctttttt acttttatgg
     6481 ctgtacttaa tgttatatct ttttcttctg tttgttcatc attgcttaat gccccagtat
     6541 catttccgcc atgcccagga tctaatacaa tcactgcatc agatagctct tccggttttg
     6601 tttgtatatt ctttgtgact ttggttccat cttcgagggc taaatatcca gtatagccat
     6661 catttgtctt gatctgataa gcattttttg tttcagaaag aaactccgcc agctctccgc
     6721 cgtttatagt tgcaagtggg gtagttagcg agtcatctgt atatagtgta cggtcatttt
     6781 ttccgatttt gattaatgtc tcttgttctg tatcatcaaa tgattccttt gtctttgtaa
     6841 ccgtactgtt cgtactgctg accgtttgtc caactatctt tttttgacca aaaacatcta
     6901 tttgaaaaca gataataatg ataatgattg caaatacaaa agctccaatt atttttttca
     6961 ttcttgactc tcacctcgta gatccagact aagggccaaa acttaaaatc agcttaagta
     7021 agcaaaaaag gggctgtaaa agatataacc gtagaaaata acattactgc taaaatgatt
     7081 tttttcattt ttggcatatc ctgaaatctc cttttgacta ttcgtggtga ttcatctacc
     7141 caataaatag tttccttcat tctacaaaca attaatttct tcgttattaa attaaaacac
     7201 ttcctggaac accgacataa taaataaaaa tataccctga taatcatgag agaataacaa
     7261 atttgtattt atgaaaaact acctatataa agaagcaaga tataacgatc ttcgtcatat
     7321 cttgcttctt cacctaacta ttttctccct atataacagc tttacgcttc tcctaataca
     7381 ccattcaaat ataaatcgat caattgcgcc acatttagcg gttcttccac aggtcttttc
     7441 gaccgagtga tgtaaggtgc taacactaag aaaaaatgcc ttgctgcgat ttcttttgaa
     7501 atcccatttc ttagcacaga ttggtttgct gaaaagattt cttcaaaagg ttcacgataa
     7561 tttttcttcc atagaaagaa aagatgatct cttgtttcct ttgaaagatt gctttcaaga
     7621 tcgtgaagca tcagaaacaa atcgaccaga tgtgtctcaa tcaaaaactg cgccattttt
     7681 gtcaaggtta cttgaaatcc cgtttcgctt gtttgaacga tttctctcaa agcttttcca
     7741 acatctttta agtggttttc taaaactgcc gtatatagtt tttctttatc accgaaataa
     7801 tgatagagat tgggctgggt gatcccaact ttttgtgcga tttctcttgt tgaagtgccg
     7861 agatatccct tggacataaa caagtcagtc gcttctttca aaattttttg ctgtgtatct
     7921 ttgttgtatc gatggaccat ttttccacct gctttttggt tattcttttt ctttcaatac
     7981 accatcttcc atcataaata ttctatcgca tttgtcaatc agccgtgtat cgtgagtaac
     8041 catgatcgtt gctttatttt tgtcttttga ttcttttgcc aagatatcga ctacttctcg
     8101 tgccttttct gaatccaaac tagcagttgg ttcatctgct agaataatgg acggatcatt
     8161 gtacaatgca cgagcgattg cgaccctttg ccgttctcca ccagatagtt cttctggata
     8221 cttgttgatc aacttatcca cgccaagctg ctcaaataat tcgtttgctt ctttttgacg
     8281 atgttcatgt ttaatcttat ctactaataa taactgcttt tttactgtca gaaaaggaac
     8341 cagattggaa gcttgcaaaa taaagccaat ttctttaaaa cgtagtttaa cgcgttcgct
     8401 ttctttttga ttactgaatt cttgatcatt gataaataca gagccactgc taggcgtctg
     8461 caatccgcct gccaaagtca aaaaagtact tttacctgaa ccagaaggac cgatgattgc
     8521 aacaaattcc cctttatcga tactcatggt tgtttctttc aatgctttaa ctacttggtc
     8581 gccatcttgg aaactttttt ctacttcttt aaattctatt gctttcatgt tcttcctcct
     8641 tcttatccaa ttgccttcaa cggatcgatt tttacgattg tccgaacaga aaatagtgcg
     8701 cctaacacag ctactactaa catcaacaag ctaatgacgc caaaaaacag ccaattgctt
     8761 tggaatggaa ccgcatcagg tagaacaagg gatgttccca ctgttcctaa tagaccaaga
     8821 agaataccaa caaatgataa aatgaatgtt tgtgccacta cagaacgcgc aataaatcct
     8881 cctgtgatcc cttgtgcttt cattacacca aagatattaa tcttttgcat cgtcagtaca
     8941 taaatgaaaa tcccgatcac aatagccgca ataacgatca aaaatccgat cataaatcca
     9001 aaagtcaata cttgcgcatt atatccaggt aattcattga tgaatttaga aattttgatc
     9061 tgttctaaat cttcaggtaa attactgatt ttacctctga caactattgc gttaatccgt
     9121 gcattttcac ttgtatcttc tttttcgaag cggatttctt ggtaagcatt gatggttgta
     9181 tagagaactg gtgagacatt gaatttggca tgatccgtaa agccagttag tttaaacgtc
     9241 ttgtcactac cagataactt aactgtatct cctatggcta gaccatattc ttctttcaaa
     9301 ctaatatcgc ctactgcctc atcatcgtta tcaaaagctt ttccttcaac aagattgggc
     9361 atgatgaatt gatttttatc gataccgaag aagctaacat tgatttttcc ttcttctgta
     9421 ctgtctttac tagtcacgac tccaggtgtt tgggcaagat acgccacttt cccaccttcg
     9481 acttcttctg ctatcttctt agtaatcatg gacatcccta gatttgaatt tgcatctttc
     9541 gaaagcacaa tcgaatctgc ctcccacttg tcgactgctg ttcgattatc ttgagctaag
     9601 ccataagcta acccagtcaa aaaaaatacc aggtaggcga tcaaaaacat cacccctgca
     9661 actaacgcat aacgtaactt tgaatgcata atttcattta atgctaaaaa catttaactc
     9721 ctcctcctga aataatcatt ttatacaaca attttatcac ttgataaatt tttggcgaat
     9781 attatgctca aaaaattctt ccgctatttc attatatgaa ttttttattc tcgattgatt
     9841 aatatttatt atatccccac acaataaaag accgtgatat ttctcacagt cttttatcta
     9901 ctctattgct atttgctctc ttagctagta ctttaaaagc taactcattg aggtagtgaa
     9961 aacgagaggt gtattcagac agaaatccaa aatcctcaac tgctagctgt ggaatcgtag
    10021 ttttgtgttt cacgatatcc gcaagcaggt tcaaatctga taataagaat tcttggctta
    10081 tcagctgtcc gccaattatt tctaatgtat ccggatggta gatcagcttt gcatttactg
    10141 ttttgttttc ttcgtcgtat tgtggaaata gatcaaattc tcctgaacac gaaccggtat
    10201 tttgttccaa gaatggcgct tcatcttctg tcagccctac agttcctaaa aagtagtcta
    10261 taatgactgc tgctgttatc ttttgtctga ggtcgtaagc ggttggctga ccactcaaag
    10321 ttagcgctac tgctcttgcc atatgtacag ctcgagtaac taaaggtaaa aagagatgcc
    10381 gtttattttt gtcaaaactg actgggacga tatctccaat cgcatagatg tccggatcac
    10441 ttgtctgcag atattcattg actatcacag tctcatcctc atacaaagcc actttttctt
    10501 ttaacaatgt cgtattagga cgagtttggg cagaaaaaat gaccatatct gcctttattg
    10561 tttgagacag aagttgtacg gatactactt tctcttctga ttttttccaa tcgatcaaga
    10621 attcatttgg ataaaaattt atttgcttag tttgcagctg attttgaaaa ctgttgatca
    10681 tatccttatc gaaatatctt gataaaacat tcggcatata atcaactaga tgaataatgt
    10741 atcctttatt tctcaaagca tctgctaatt ccaatccaat gtaaccagca ccgaagatca
    10801 ctacttcttt tgcttctttt aaagcggcaa gtacttgctt agattgcact agatttttat
    10861 aggtgtagac attttcagct tcaatttgtt tatacaaggt gacaggagag gaacctgtcg
    10921 ctacgattaa tttatcatag gttatggtct cgttttttcc ttctttggat gcgaggatga
    10981 gctgatgttt gtctggatca agttcagtta ctctccattc ggtgatcagt tttgccccac
    11041 tttcttgaag agtccgcatg ttatgggcaa cttcagctaa actacgtatc tttcctttta
    11101 aataaaggtt gattccattt gaaacatagc ctagtccatc tttatgatct tcatcgatca
    11161 gtaaaacttc tgctttcggt tgaagttttc ttagatttag ggcagctgtg atgccttcat
    11221 gggaagcacc aatgatcacg attttcatgt tttattcatc cttaaattca atttttctaa
    11281 ataacattct tttaaaaatt ggttcaaata ggggaaatca taattatatt cgttgctagt
    11341 gaatacaaaa actttagcgg acttctgtga cgggtactct ttcaattggc tagtcaaaat
    11401 gatatcatac tgcttttctt tctcgtattc gactaaatca tatttgatcg tattcagcaa
    11461 ttgttcctgg atgatttctt tcacgatatc cgccattagc ctttcataag gaagatcata
    11521 agcaatttgc agacgtaatt ctaactgctt gatcagcgtc cgtaaaatag atgcatagcg
    11581 agcaaataat tcaaagcggg cattttcctc gtctacttca gtaaaatcaa caatagcaaa
    11641 tgtttgtctg accaatcttt cacataaaac caggaattgt ttatcttcta cttcagttaa
    11701 ccgctgtttt aatccttctt ggccaaaaat attcatccat cctctaaaat aggcaaaatg
    11761 agcgtgattt tgcatcactg tcaacgcaat cttttgcgaa atctcttctg gaaaaacata
    11821 aaaagagaaa tattcttcta tcgattgttt aaacgttcga ttcaattgag ctactacgtc
    11881 gtctttgtat ttttcgctta tcaaaaactc ctggacatgc gagttatgtg tatctaatgt
    11941 aaagttactg atcatgaaaa gataaaaaac caacgtttca ttttgattcc aaggaaaggc
    12001 atattgaaag aagtaacggg tcaaaaatgt atggaggtcc aataaaaatt gtgtcgtttg
    12061 aaacaatcca acaggaagat tgctataatc tggtttggat tttttcgtac agcgcattcg
    12121 cttcttcata atggaaagcc atatttcaaa tttcaatcgt ccttcttctg taatagcaaa
    12181 actaaactct ttttgcagca gatctaaaaa agtgtttcct gataaatcac tgtattcttt
    12241 tttgttttca agatagggac gtccaaacca aaataattgg aaataaaaat aacgaatttg
    12301 actttctgga ccggtcagcc gcccgctttt tattttcaga tgaaactcat ttaaagaatc
    12361 atttaattcc ttcactctgc gagacaaagt agccgaactg atattaagtt ccatgacaag
    12421 tttctgccga tcaaatgagc cattttccaa cagataaaga agtatctgat attttgtaga
    12481 atagcgcaag taacaatgat aataaacatc gactgctgct atatttccat gtctgattaa
    12541 taaaatattc cattcttgga taacaatttc caattgtttg atattttcca agttagcttg
    12601 taaataaaac agatcttctt tcaaggtagc ccgatcaact gataagcggg taagtacgtt
    12661 attaacggaa acctctcctt tttcctctaa taaaagttgg aaaagtttga tttgccgttg
    12721 ttcatttttt tcaagcaaac tgaaaagttc caagtccact ccttcaagca tttctgattt
    12781 taacagcaga agtttccttc tcatccttga aaaagaggat ctgtgacagt tatcacagat
    12841 ccatacaaat tctaatcact tagctggttg gcccagctgt cagttcccat gtgaccatgc
    12901 cttgatacgt tgcgccaggt tgagcattcg tactgctatt tacctctaag agtaaaccat
    12961 cggtatcggg aaagtcaacg agaacctctc ctttagctgt tccatcactc tcataaatca
    13021 acgtgttttg accttgctga atcaattgct ttgatccaga atgattgaag tataattttg
    13081 ttccgcttaa ttcttgacct tgatcattct taaataaaga agtcaatgta cctgtcagtt
    13141 gccaaggaga cgtgcttgga agccgagtat cccgcacatc aaaagaaaaa tcgccttttg
    13201 attcataata acttgtcttc aacggtttgg ggagatcgtt accaaatgta aaatcatctg
    13261 gtacattaac taatgttaat tcttgtttgg ggacaagaat tgttttagca gatgtttgga
    13321 caactgggtt aggaagctta gcaccatctg ctaatgtaat attgctggaa agtacagctg
    13381 cctttgtcag tatgctgacg gattgtaaag aagtatctac agtatttaat tgatagttgt
    13441 aagtaagatg aatggtatct cctattttgc tcaatagatc tgctgggaca gaaactcctt
    13501 gttgaatgcc ggtatctgcg atagttgtac catttaattt taaggaacca ttgatataag
    13561 tagtattttc aggaagtgag atcgtgattt ttttgtcggt tatcgcatcc ccagattttg
    13621 ttaacgtgaa tgtcacattt tcttgaatcg tatctcctga tagagcagac aggggatctc
    13681 ctgtctcagg ttgtctgcta aggtttttta gctgactatc tgcactcaca gatggaaaat
    13741 tagccactgt tatttgagga agagtggtga ttccagcttg ttcagactgc catttgtacg
    13801 caaaagacgg atacatgtta gtaagtacat cgtccaccaa gcgataatca aaataatatt
    13861 gccctgaagc caatgcaggt agtgtagaag tagctgtatt tacacccccg ttatttgatt
    13921 ggatcgtttg gaaaagttga tctgtccatc ctgtcgctaa tttttgggtt cctttcgaat
    13981 aaagcctgta atggatcgaa ccctttttta gttcagttgc tgaccaattg gccgtgactt
    14041 ggtacgatgc agaagaaagg agctttcccc actgactatc ggctgttaag tttgttggta
    14101 aaaccgttcc gtctagcttg tactgctcca atacctttaa agcaacagga gaaagcaatc
    14161 cgccttgcgc atctttccca ctgtacacga tattggctgt ctgattcaat ccacttgtgc
    14221 ttgctggagt atgagtcagt attgctcctt gtgtaacgtt ttttcctgta aaatagttga
    14281 ctacgcgatc aaagcgacta aatggcaact gatcaaaacg tccttcagtc agccccatcg
    14341 tcaatgtccg actattggta gagcctggaa ggttctggtt atttccatcc acgtattctg
    14401 tggtatcagc aaatgtatta tattttcctg ttgaatcact tggtgagatg taactattcc
    14461 acgtatttat ctctgcactt gcagaaaatg catatgctcc tggattcaag tttccgccag
    14521 ttaatttagg ataaaatcta cctgcagctg gagctaaaat cgaattgaac atacgatctt
    14581 ggataatatt attcgaatca taaaaatcaa cagaaaaaac ggccttttta gacatagaag
    14641 gttgtaattt acccttggtc aaaattttgt ttggactcgt atcatattta tctccgtttt
    14701 ctgtcatctt cgcgacatta gctgtaacat caaattgaag cgggatatcg tccacgttga
    14761 attcgcctcg cgtaataata gcaataatat ctgtcaatag agtatttgta tcccacgacc
    14821 ccatcccaaa acgcaaggta tggcttcctg ctgataataa acttccgcta gtaaccttga
    14881 tgggggcacc ggcaatatac aagtaggcgt tgttccagtc aattgctttt aatacatcac
    14941 tgcttgatac atgttccggc aaattgatga tcgccacaaa tgcaaatcct tgtgcagtga
    15001 tattcaccaa atcaagtaca tttgttagtt tcacatggct ttcccaatga aaacggaccg
    15061 tgccaccttt ccaaggatca tttgaaccat cgttcaaata agtagcagtc aatttaggca
    15121 attggttagc cccagttagt tggacaagct tcccgccatt gactgcacta cgtccaccat
    15181 ttggaggtac gatattttca tacgtgtatg gatcataaga aatggcagga ggcgttgctc
    15241 caacagcttc tgccgaaggt gaaaagataa atatacttaa aacaaaaagc gaagtaaaaa
    15301 agaaggagcg aaaaaatgaa aatttatttt gtagcaacat gttcatcctc cttttccttt
    15361 cttctccata acaacaacca cgcaagaagc cataattcta taccaatgat caaataaaga
    15421 aaacggatct tttgttcatt cgtctccggg aatacgccat gttgattaga ttctttataa
    15481 atcggtagct gtgtctgctg ttttgatgcg ccattgactg aaccacttgg atgcggaatt
    15541 ggacttagat cataaaatgt aatcgtacct gaacttttgg tatctgcttg gactggctct
    15601 tgccagctga agaacagcaa acttgcaata atccctccca ataagatcga tgttttcaac
    15661 gaaatcacct catcatgttg tttctccttt tttctttttc ttgtggaccc acaaaaacag
    15721 caaaatcaac cataacacta gaactgcagc aaccaatcct gcataaagcc atgggaaaga
    15781 ttgttcttgt actgcaaaaa cggaattttc atttactttt tgtgcttttt cttttttgat
    15841 cgtaaagttt tgtgtaaatc gccattcatt actgtcgcct gattttgcta tgatttctgc
    15901 gcggtaatcg ccggcttttg ctttatcacc agctaaatca agattcaagt gaaaattcga
    15961 atttggtgca actaaaaatg cattagacgt attttcaata taaagctgat tttcataaaa
    16021 gatcttgctc gtaaatatca aattgctgat aatcctaggc tgaggatttt ggaaacgaag
    16081 ctgaacagtt ggctttgcgt tgacctcgtc caaatctcct gataaaagtt tcagatccgg
    16141 ttctatcgtc tcttgacttt cttgtaaaac gactgcgaga ttataagcaa tacgattttt
    16201 gatttgtgct ccttctgtag cattttctgt ttcagcttct tctttaggtg aaacatgaat
    16261 acccgctaat acacgtccct taaagctttt tgctgggatt ttcacttcta taggtacagt
    16321 tgtttctgca tgtgctgata cattgatttc ctttttcgca atgagcaaaa cgtcacggac
    16381 atcaaacggc aatgtctcat ctaacgttgg gtttttttga ctataatcga tcgttccacc
    16441 atcactcgtt accgccggat tgacagagac ctcaaatgtt cgttcttcat ctgaagaatt
    16501 gctgataatt acttctagtg tctgattttg ttccggtgcc actaataaat caaaataacc
    16561 gattgcttta tctatttggt tctctggaaa taccggtttg attccaaagt ctcctgctga
    16621 atcctttgct tggctgattg acggaaaaat gaaaaataga cttaaaatag aaaaaaacga
    16681 aacaaacagt cgtttacttt gattcattgt ccaccacatc ctttcaacaa caactgcggt
    16741 aaaaaatcct tcacctatag tattttcacc gcagtttgtt gaatttattt catactctat
    16801 gctactgggc cagccacgag gttccaagtc aaatcagccg tgaatgttcc agctgctgct
    16861 ttcggcactt tcagtgtgat atttgctgga tcataaacag aaagccatgt tcccagacct
    16921 tgatcttttg ctgctgtaaa tattggtgtc gctgcacttg ctttactcaa cccagttaca
    16981 gtttgactag ttggtccagt ggattcgtta tctgacgtac ttttaacagt ggatgttcca
    17041 aaatctaatt cagcgccttg caaaacttgt gaaccgttta caaaatctga aaccgaaact
    17101 gtcacgttcc acccttgtgc ttggccatct gcacctacac ctcgacgatc tgatacttgg
    17161 agataaggtg tatcgttttt tgaagcgtct acttgatacg tttttgttcc gctttcaatc
    17221 tcgtgtgtac caaatggcaa ttcgggaact acatccagtg ttaaaggacc tgtctggcca
    17281 gttgctggat tttccggatc tgctggctct actggttttg aaggatcagt agggttaaga
    17341 ggctccacgg tatttgttgg ttcagtaaag gtaatatctc cttttgaagt cgcttgaccc
    17401 tcagcaaaag ctggtgttgc caagccgcct agaagaagtg ttgaaagtaa taatgtccca
    17461 atcatgttct ttttcataat aaatcactcc tttttcttag tgacctaagt atagtagaga
    17521 cagtatgcaa aaaatcctca tgaattcatc cgcttacttg aaattatgac cctttttgta
    17581 taaaagagaa aaaaacaata aaatcgttta catgaatatt cttccttttt tattaa
//
LOCUS       DACEZN010000047        17518 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEZN010000047 DACEZN010000000
VERSION     DACEZN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.047"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..927
                     /locus_tag="IXH60_001231"
     CDS             <1..927
                     /locus_tag="IXH60_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1990170.1"
                     /translation="GSGNGYKVAERILPVLKNKHIDYTLHYSEYKGHDAEIAETLAKE
                     TLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVAYIPAGSGNDFARG
                     AGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGIGLDAAIVHATNHS
                     STKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSNAFLCTATKHPFFG
                     GGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSKYFHHYTTNRLRIV
                     STTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1026..2198)
                     /locus_tag="IXH60_001232"
     CDS             complement(1026..2198)
                     /locus_tag="IXH60_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990171.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2185..3330)
                     /locus_tag="IXH60_001233"
     CDS             complement(2185..3330)
                     /locus_tag="IXH60_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990172.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3344..4276)
                     /locus_tag="IXH60_001234"
     CDS             complement(3344..4276)
                     /locus_tag="IXH60_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990173.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4393..5472
                     /locus_tag="IXH60_001235"
     CDS             4393..5472
                     /locus_tag="IXH60_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1990174.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5469..6098
                     /locus_tag="IXH60_001236"
     CDS             5469..6098
                     /locus_tag="IXH60_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1990175.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6208..6435)
                     /locus_tag="IXH60_001237"
     CDS             complement(6208..6435)
                     /locus_tag="IXH60_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1990176.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6538..9126)
                     /locus_tag="IXH60_001238"
     CDS             complement(6538..9126)
                     /locus_tag="IXH60_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1990177.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9146..9805)
                     /locus_tag="IXH60_001239"
     CDS             complement(9146..9805)
                     /locus_tag="IXH60_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1990178.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(9854..10357)
                     /gene="trmL"
                     /locus_tag="IXH60_001240"
     CDS             complement(9854..10357)
                     /gene="trmL"
                     /locus_tag="IXH60_001240"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1990179.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10578..11444)
                     /locus_tag="IXH60_001241"
     CDS             complement(10578..11444)
                     /locus_tag="IXH60_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1990180.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11553..12194
                     /locus_tag="IXH60_001242"
     CDS             11553..12194
                     /locus_tag="IXH60_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1990181.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12256..13617)
                     /gene="mgtE"
                     /locus_tag="IXH60_001243"
     CDS             complement(12256..13617)
                     /gene="mgtE"
                     /locus_tag="IXH60_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1990182.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13635..14534)
                     /locus_tag="IXH60_001244"
     CDS             complement(13635..14534)
                     /locus_tag="IXH60_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1990183.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14536..15333)
                     /locus_tag="IXH60_001245"
     CDS             complement(14536..15333)
                     /locus_tag="IXH60_001245"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1990184.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15311..16015)
                     /locus_tag="IXH60_001246"
     CDS             complement(15311..16015)
                     /locus_tag="IXH60_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1990185.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16146..16721
                     /locus_tag="IXH60_001247"
     CDS             16146..16721
                     /locus_tag="IXH60_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1990186.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            16839..17516
                     /locus_tag="IXH60_001248"
     CDS             16839..17516
                     /locus_tag="IXH60_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1990187.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5173 a         3773 c         3121 g         5451 t
ORIGIN      
        1 ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa aaataagcat
       61 attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat tgctgaaact
      121 ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat cgatactttc
      181 ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga tactttctat
      241 cagatggaag tagaattccc agttgcttat attccagcag gttctggaaa tgattttgct
      301 cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt agcagctcaa
      361 tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa aaaaggaata
      421 gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac aaatcattct
      481 tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt attctctatt
      541 ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg aggaaaacaa
      601 ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt tggcggagga
      661 atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt cgtggtcgaa
      721 cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa aaaacaattg
      781 aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc aacaacaccg
      841 caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat cacaataacc
      901 acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct acatctggaa
      961 caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct ttttttacta
     1021 tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt acccaaaata
     1081 aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt gaaccaaaaa
     1141 gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt attttgaaca
     1201 aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa gtattctgct
     1261 ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt gatcccaccg
     1321 ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat gatcggcaat
     1381 aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga ggtgtggatc
     1441 actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta actaatcaaa
     1501 ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg gtttcgttta
     1561 ctgatctctt tgcgattaaa cgctagattg atgacgctga aaccgctaaa aatggataga
     1621 aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt catccgctga
     1681 ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg tgtctgttcc
     1741 atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg caaaaaggtg
     1801 ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg tatcttgatc
     1861 gtcgccatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg taagataatg
     1921 tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc atccaatgta
     1981 acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg cgactcttct
     2041 tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc aatattttgc
     2101 gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt cgatttattt
     2161 gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc tttccatcca
     2221 agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat tggtataaaa
     2281 actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca agttgacgag
     2341 atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt gttccgaccc
     2401 catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc tatccttttg
     2461 caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa tcgcacacaa
     2521 cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt ctacttgata
     2581 gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg taaagcttgc
     2641 tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca agcgaatacc
     2701 atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca tcgtcccgta
     2761 gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca tattacgacc
     2821 tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg gcgcgagatc
     2881 aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta aaaattgatc
     2941 catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga gcgaaacaga
     3001 tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt cattcgcttc
     3061 tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga cttgttctaa
     3121 gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa ttccagtatt
     3181 aatcgtttca agattgccac cttgatcaat ctcgccaaag gccgccataa acatcaatcc
     3241 taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca gcagtatttt
     3301 tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct ctcaaatcct
     3361 tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct atttttccgt
     3421 aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta tttagttcag
     3481 tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct attgttcgct
     3541 gaatcgctga caacacattt tctgggagta atgggatgtc gatcgtcaaa cgatccgccg
     3601 tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt ccttgatcca
     3661 tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt gtatagacaa
     3721 tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc cgactttggg
     3781 ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg gcaatcccac
     3841 atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga aacgaaacat
     3901 atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt ttctcttttt
     3961 cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt tcgtcaaaga
     4021 ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt tcaggagtca
     4081 ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa agcatacagt
     4141 tgatggcagt cgatttccca ctgccgtttg gacctaacag acctaagact tctccttctt
     4201 gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg actaatttat
     4261 caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct gactataata
     4321 aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt tgtcacaatg
     4381 aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct actggttatc
     4441 tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta tttattgatc
     4501 gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg gattttccaa
     4561 gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct gccagcgacg
     4621 atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt aggttttttg
     4681 ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt aattttgctg
     4741 tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga gaaaaagtac
     4801 attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca aaataaagca
     4861 ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg gaaccgaatt
     4921 gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt acaaattggt
     4981 gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa tttaagtgcc
     5041 accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca tgagacatcg
     5101 atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc tgttacgatt
     5161 gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt aatggtcatc
     5221 aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt tttacatttt
     5281 cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag cccacaaaaa
     5341 aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga acaaaaggga
     5401 caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt accaagggag
     5461 ggacaccaat gataacagta gtcattattg atgatgatcc atttgtgacg aaatcgttac
     5521 aaaccatttt agaaagcacg aaagagatcc gtgtgttagg cattggtcat tgtgccaagg
     5581 atgcgctagt cttatacgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc
     5641 ctaaacaaac gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc
     5701 ttttgctttt gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg
     5761 tcaaagggta tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt
     5821 ataatggaca ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga
     5881 ataaaccgac gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca
     5941 tcaaagaagt agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg
     6001 acggcactgt tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca
     6061 cgcaattggc tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac
     6121 ctgaatgatg ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg
     6181 caaaccattt cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg
     6241 attgttcggc gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga
     6301 ttcttcacga tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat
     6361 ctcatctaac tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc
     6421 ggtttcttta gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga
     6481 aaaaacaaaa gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca
     6541 agcatttttt gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc
     6601 tactaactgg ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc
     6661 tacctcttct ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc
     6721 cattacttgg tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt
     6781 tacaacccgt ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga
     6841 atcttcataa gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat
     6901 ccttgatttc aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc
     6961 ttgctcttcc cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa
     7021 attacgctga agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc
     7081 actgccttgt gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt
     7141 tcttttataa gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc
     7201 agaatctttt gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa
     7261 ttcgggagta ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca
     7321 atgtacttcc ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa
     7381 cgcatcgatc cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg
     7441 gaatccctta tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc
     7501 atcacttcgg aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc
     7561 ttctttgatc tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg
     7621 attgagttcc attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg
     7681 accgacagag ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat
     7741 cgctttgaat aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa
     7801 aagtccgcct tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc
     7861 cagaagacga tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc
     7921 tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc
     7981 caattccagt ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt
     8041 ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc
     8101 ttgttcttgc gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt
     8161 ctgcacttct ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga
     8221 attaccgatc ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg
     8281 ttgtatcttt ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat
     8341 ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg
     8401 tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg
     8461 aattcttctc ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt
     8521 gaacccgatt ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa
     8581 agactcgttc ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg
     8641 attcagtccc atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat
     8701 ttctcttttt ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat
     8761 tttttcgata gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat
     8821 tcctgggaat ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc
     8881 ttgctcatat ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc
     8941 ataaaaacga taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc
     9001 tttttcacgg taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt
     9061 actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg
     9121 ctgcatcgga agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta
     9181 aaaaatctgt ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag
     9241 tctctaaaat cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct
     9301 cttttcctgt catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa
     9361 gtgggccatc tttatgtcgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg
     9421 ccgattcaat cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca
     9481 aattcggcag attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag
     9541 tacccagccc tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct
     9601 ctttgttgat accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt
     9661 taaaaggaac atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca
     9721 aaagcggaga atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc
     9781 catgtcgggt aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc
     9841 tataataaac tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag
     9901 ggaatccttg ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca
     9961 atgagcggac atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca
    10021 taaatgtttc tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat
    10081 cagcaaaatt ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac
    10141 gattacctaa aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat
    10201 tccaataatc caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct
    10261 cgattaaatg aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg
    10321 cagggattaa tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta
    10381 tgttcgtttc tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt
    10441 gtcagtcact gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag
    10501 gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt
    10561 tttgcaacct ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat
    10621 aattctatgt caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc
    10681 tcttctacct gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt
    10741 atctcaaatt cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg
    10801 gctactacac gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga
    10861 cgtagttcct ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag
    10921 agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat
    10981 gtttgatcag caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat
    11041 tgttctgtag ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc
    11101 tttttcaatt ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc
    11161 gagacttctt caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat
    11221 tggatcattc tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg
    11281 gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc
    11341 aagccattat cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga
    11401 ttttctgcca aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc
    11461 tgttttatct taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct
    11521 agaagaaagt gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact
    11581 acacatctat tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact
    11641 tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg
    11701 gggaaaaaga tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata
    11761 acgatatcga attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg
    11821 atggcgtagt atttggttgt cttactcctt caggctggct agacgaagag gccttagaac
    11881 tattgatcga aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc
    11941 ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct
    12001 tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat
    12061 tgattgccta tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga
    12121 atgcagcaca tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg
    12181 atcttcagtt ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca
    12241 aatgtttttg tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat
    12301 cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc
    12361 aaatcctaga cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat
    12421 tgcaaatagc atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc
    12481 tacgattaga aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc
    12541 agccaaaaca agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac
    12601 agccacagct agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa
    12661 gactgctaaa atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt
    12721 cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac
    12781 tggattttca ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc
    12841 ttcatcatca ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc
    12901 ataatcggta acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc
    12961 ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc
    13021 gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg
    13081 attaacgaca tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta
    13141 catcgcagag cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc
    13201 gccagccgtt tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga
    13261 catcaaactt aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc
    13321 attatctgta tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag
    13381 gtattctttc atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt
    13441 aggcgacaga taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg
    13501 cccctgctca tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc
    13561 ccttaaatct tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc
    13621 aatctcacct cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa
    13681 ggctgcttga actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa
    13741 tgaagggctt gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc
    13801 agggcacagc caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc
    13861 tggatatcaa ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc
    13921 gaacgtcctt catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc
    13981 aaatcgatat gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat
    14041 tttttcacaa acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc
    14101 tttgcaaaaa gcatcaagcc agatgtatcc cgatctagtc gcgtcaccac atggattact
    14161 tggttttcgt aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt
    14221 ggatggtatt gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt
    14281 tcatcttcat acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct
    14341 tcagcaggaa tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct
    14401 acttgatcat taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt
    14461 gagatccctt tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc
    14521 catgtaaact gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg
    14581 catatgtcga taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg
    14641 gatatttgct tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc
    14701 caattttatt tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa
    14761 cactcgatta ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat
    14821 cacagcaccg ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa
    14881 tccatctcct ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt
    14941 acgattggcc cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc
    15001 atccaaataa ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt
    15061 tcgtaagctg tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa
    15121 atgacctgta tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa
    15181 cgccgacaat aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc
    15241 tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt
    15301 tgcttcttta ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt
    15361 tcttcaaaag cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc
    15421 gctgtgcttc ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag
    15481 cttctgccgc acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat
    15541 aattcagcga atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt
    15601 ccgccaaaat tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat
    15661 aagaacggta gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca
    15721 tgtcttttct tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca
    15781 tgatccttaa cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc
    15841 gtaattgtga ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa
    15901 tcgggacatg tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca
    15961 attctgatac agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg
    16021 atacctctac ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta
    16081 ctcttgattt atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg
    16141 atcaaatgac acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg
    16201 agatattcat cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt
    16261 atttcgatac cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg
    16321 ttggtcatca agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa
    16381 cggatacctt tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt
    16441 caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa
    16501 ccggaaagct tacaacaaaa cgagctgaat tccctattga agaaggactt ttagctattg
    16561 atgaaagctg gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat
    16621 ctgtgggaaa aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg
    16681 ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa
    16741 ggcacttgag ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag
    16801 tcactagttg tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg
    16861 ttaatccttt aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag
    16921 aagagtacgg gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt
    16981 tgcagacaat tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac
    17041 gtaatcactt attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc
    17101 aaggaaagaa aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg
    17161 acataccata ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga
    17221 atatgtttaa agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa
    17281 aaattgctcg ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct
    17341 gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca
    17401 cagagctgtg taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata
    17461 caaatgattc tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaa
//
LOCUS       DACEZN010000048        17404 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEZN010000048 DACEZN010000000
VERSION     DACEZN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.048"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(22..633)
                     /gene="rpsD"
                     /locus_tag="IXH60_001249"
     CDS             complement(22..633)
                     /gene="rpsD"
                     /locus_tag="IXH60_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1990188.1"
                     /translation="MSRYTGPSWKISRRLDISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            949..1746
                     /locus_tag="IXH60_001250"
     CDS             949..1746
                     /locus_tag="IXH60_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1990189.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            1774..2409
                     /locus_tag="IXH60_001251"
     CDS             1774..2409
                     /locus_tag="IXH60_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1990190.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            2429..3202
                     /locus_tag="IXH60_001252"
     CDS             2429..3202
                     /locus_tag="IXH60_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1990191.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            3610..4407
                     /locus_tag="IXH60_001253"
     CDS             3610..4407
                     /locus_tag="IXH60_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1990192.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            4385..4798
                     /locus_tag="IXH60_001254"
     CDS             4385..4798
                     /locus_tag="IXH60_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990193.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            4782..7427
                     /gene="mutS"
                     /locus_tag="IXH60_001255"
     CDS             4782..7427
                     /gene="mutS"
                     /locus_tag="IXH60_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1990194.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            7445..9553
                     /gene="mutL"
                     /locus_tag="IXH60_001256"
     CDS             7445..9553
                     /gene="mutL"
                     /locus_tag="IXH60_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1990195.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            9575..10135
                     /locus_tag="IXH60_001257"
     CDS             9575..10135
                     /locus_tag="IXH60_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1990196.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(10220..12208)
                     /gene="treP"
                     /locus_tag="IXH60_001258"
     CDS             complement(10220..12208)
                     /gene="treP"
                     /locus_tag="IXH60_001258"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1990197.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            12431..13144
                     /gene="treR"
                     /locus_tag="IXH60_001259"
     CDS             12431..13144
                     /gene="treR"
                     /locus_tag="IXH60_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1990198.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            13221..13667
                     /gene="msrB"
                     /locus_tag="IXH60_001260"
     CDS             13221..13667
                     /gene="msrB"
                     /locus_tag="IXH60_001260"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1990199.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            13837..14439
                     /gene="ruvA"
                     /locus_tag="IXH60_001261"
     CDS             13837..14439
                     /gene="ruvA"
                     /locus_tag="IXH60_001261"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1990200.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            14452..15453
                     /gene="ruvB"
                     /locus_tag="IXH60_001262"
     CDS             14452..15453
                     /gene="ruvB"
                     /locus_tag="IXH60_001262"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1990201.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            15482..16060
                     /locus_tag="IXH60_001263"
     CDS             15482..16060
                     /locus_tag="IXH60_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1990202.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            16112..16594
                     /locus_tag="IXH60_001264"
     CDS             16112..16594
                     /locus_tag="IXH60_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1990203.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            16711..17085
                     /locus_tag="IXH60_001265"
     CDS             16711..17085
                     /locus_tag="IXH60_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990204.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         5773 a         3106 c         3813 g         4712 t
ORIGIN      
        1 ttttcaagaa agcttttgaa attataagct ttggttgtag tattcaacga caagagcttc
       61 gtcgatttct ggatatagtt cgtcacgttc tggtaaacga gtcaatgaac cttccaattt
      121 ttcagcgtca aagcttacga atgctgggcg tccaacagtt gcttctacag cttcttttac
      181 tgttacgatg ttttgtgatt tttcacgaac tgagatcact tgacccactt ctacgtggta
      241 tgaagggata tcaacgcgtt tgccatcaac taaaatgtga ccatggttaa ctaattgacg
      301 tgcttgacga cgagtcgttg caagtcctaa acggtaaaca acattgtcta aacgttgttc
      361 tagtaagatc atgaagttaa caccatgttt accttctttg attttgctag cttttacgaa
      421 caagttacgg aattgacgtt cgttcattcc gtacatgtga cgaagttttt gtttttcagt
      481 taattgcatg ccgtattctg attgtttgcc acggctatta ggtccatgtt gacctggtgc
      541 gtaagggcga cgtgctaatt cttttcctgt gcctgacaat gagatgtcta agcgacgaga
      601 aattttccat gatggtcctg tataacgaga cattaaaatt tcctccaatg aatataagtt
      661 tttggagtaa aataatccgt tgaaaaattc ataatcatat agtttgttct tcaatcttca
      721 cctttgcagc cgcggttaca caatcgaacc cgagacgggg caacaaacta gggatgagtt
      781 attatttttc tgctgcatta ttttacacaa attacagtat atgatagatc atccacgaat
      841 gtcaagataa ttagtacata aaaacgcttt acaaacctag gctgtcttta taaaatgaga
      901 gggtatgtgc aaatggaaaa aagcgaagga agtttaagga gaaaatcaat gaatccggta
      961 tcaccattat ttgagaaaat cgatgcttca attgaaaaga aggcgaatct ttttgaaaac
     1021 agcttttcac gttacgctgt tcgagcggtt ctcgcctgtt tgtttttgac tttaggaacg
     1081 gctgtggcat ttgcgatcgc gatgaagggg gaagatatcg cgcatggtct aggaaagatt
     1141 ttatacgcat ttatgttcag ctggggtctg gtcatgattc tatacatgaa cgccgaatta
     1201 ggaacatcga atatgcttta tatgaccgtg ggtgtttttc ggcagagact tgctttaagt
     1261 aaggcagcca agattctatt tgcctgtatt tttttcaata tgattggcgg tatattatgt
     1321 ggatttctaa tttctatgac tacgccattt caagatttaa cgttggacaa ctttatgtta
     1381 gaatcaatag cagtaaaact agcaaaacct tcagcaacga ttttggtgga agggatgttt
     1441 gcgaatatcg ttgtcaatac cgctgtactg atcagcatgc ggatgaaaga ggatgtcgga
     1501 aaagtgatca cagtcgtatt catcattttc atttttgctt ttttaggata tgaacacgtg
     1561 atagcgaatt ttcctgcctt tacgcttact tactttgctt ctcacggaca gatggatgga
     1621 atgacagtca gcaatgtgtt acataatttg ttttttgctt tggcaggaaa ttatattggt
     1681 ggcggtttag taatgggact gggatatgct tggctgaatc agtcaaaatc atcctatgtg
     1741 gactaaaaag tcgttcatta aaggggaagt gccatggaag ttttagtttt tccaaaaaat
     1801 gaggaaaaag cgttacaaat ggaaaagtac atgaaaaatc ttttttcctt tgctggagta
     1861 cctaagccgg aacgcgctct tttacaacaa ccgttactgg atgagagcaa aaatttatct
     1921 atttctgaag ttttagcttt tgttcattat tattatgaac aaatcgaaag agagtatcag
     1981 tatgtagcga tcgatttagt ccagaaaaac atcaaacggc ttacatttga agacttagtc
     2041 gtattgttgc cactagttca gaaaaaagaa tggtgggaca gcatcgactc ttggagaaaa
     2101 gtatacagca catggtgtaa agcctaccca gagcagcgtg aagaagtctt tcaattgttt
     2161 gaaaaaaagg aagatttttg gctacgtcga atagcaatca cgttgcaatt aggttataaa
     2221 cagcaaacag acacaggact attgatccga gcaatagacg aagatcgtca aacaaaggaa
     2281 ttctttatcc aaaaagcaat tggttgggca ttacgcgatt atagtaagac cgatgccgat
     2341 tgggtaaaaa gacagctgac aacagatttg agtaagttag ctgttcgaga ggcaagtaag
     2401 tatttataag aaagcgagac gacaagagat gagacgcaaa ttatttgctt ttgatattga
     2461 tggaacgttg ttagattcaa acaaacaagc tttagatagc acaagagaag cattggaaaa
     2521 attacgcaaa gccggtcatc tggtcacgat tgctacaggg cggagccgat ttcatgcgca
     2581 agagatcatt cgcgatcttg attttacgaa ctacatttta tgtaatggag cggccggttt
     2641 tttagaccat gaacaagtat ataagaattt actagatcgc gaccagttgg atgcgttcgt
     2701 tcaagaagcg catcatgagc agatcgatac agcttttgtc agcttagaca atatcaagcg
     2761 ttcttcttcc aatgatgtaa cggtgatgga agaagcgatg cattctttcg gtgctgtctt
     2821 gcctgaatta gatcagtttt ttgcagagga acaagaaata taccaagcgt tagcattttt
     2881 cgatcaaaca tatgatggga agttctcttc atatgataaa attcgatttg tccgctggca
     2941 tgagaacagt gtagatgtcg tacctaaaaa tggttctaaa gcagcgacaa ttctgcactt
     3001 agcagaacaa gtaggcatcc ctcatgaaga catcatcagc tttggcgatg gacaaaacga
     3061 ccgtgaaatg ttgcgcatgt caggaattgg gatagctatg gggaatgctg tgccagaagt
     3121 acaagcagaa gctaagatgg tcactgatac aaacgatcag gatggaattt ggaaggcatt
     3181 gaaagagtta agcgtgattt aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa
     3241 gcccagccaa acaaaaatag ctcctcctat ttttcgtttt tctgggctta ttaccgcaga
     3301 tgcaagcttt cgaacaccgt ttaaggaaga ggtcgtgaca gaagtggtca gtttcaagaa
     3361 ataagaaggg atttacgaaa attgcttctc aaatttttgt aaaattacgg cttatttctg
     3421 aagaagtaga tgaaacaacg tttattcggg aaatgggact gtgacaagat gtttgtcata
     3481 gtcccatttt tttattttat ttttcaaaac taagaaatac caggaaagag ttccttttgt
     3541 ttgacctttt ctttcttatt gaaaacaact ataatataga gtgatgttaa aagagaagag
     3601 gaatttagtg tgcgggtatt gtttattggt gatgtagtag gttcaatggg acgagagatg
     3661 attactgaat atcttcctag attgaagaag aagtaccgtc cgcaagtaac gattgtaaat
     3721 ggtgagaatg ccgcttctgg acgtggaatc acagaaaaaa tctataaaaa atttttgcaa
     3781 gacggcgtag atgtcgtgac gatggggaat catacgtggg acaaccgcgg aatctttgag
     3841 tttgtaaatg aagctaaaaa gatggttcgt ccggcaaatt ttccggaagg gacgcctgga
     3901 caagggatgg tctttgtcaa agtgaaccaa gtcgaactgg cagtgatcaa catgcaggct
     3961 cgttctttta tggtggactt ggatgatcct tttcgtaaaa tgaatgagct ggtagaagaa
     4021 gccagaaaac gtacgccgat catttttgta gattttcatg gagaaacaac aagtgaaaaa
     4081 caagcaatgg gctggttttt agatgggaaa gtttcagcag tggtcggtac acatacacac
     4141 gttcagacaa atgatgcgcg aattttgcca agaggaacag cgtatctgac agacgttggg
     4201 atgacaggtc cctatgatgg tattttgggt atgcgcagag aacctgtaat cgaaaagttt
     4261 ctgactgcat taccaaaacg ttttgaagtt gtggaacaag gccgatcgat tttaagcggc
     4321 tgtatcttgg aattggatga tacgacaggt catgcaaaag aaatccagct gattcagatc
     4381 aacgatgatc gaccgttcat ggagtagaaa gagaggcagt tttttgcagc aggagccgga
     4441 aattcaaaaa gaagtaagga aactgacaaa acttttaaga gagaatgaaa cgatcattcg
     4501 ctacaaagaa cttgaagaaa agatccagca aaaccagtat ttagctgagt tgagagaaaa
     4561 aatcaaacaa gcgcaaaaag atgctgttca ttttgcccac tatgacaaac cagcagcaga
     4621 aaaagaagca atcaaacaag cggatcaatt catgcaagaa tttgatcagc atccgcttgt
     4681 tgtagcttat agaaaacagc tgttggaagc agatgatctg cttcatcatt taacaactat
     4741 gatacaagaa gaaatcaatg gacaaataga ggaggaaaaa catgcctcaa aaaactaaaa
     4801 atactcctat gatggaacaa tatctaagca tcaaggccca ataccaagat gcttttttgt
     4861 tctatcgttt aggagacttt tatgaactat tttacgaaga tgcgatcaaa gcgtcacagc
     4921 ttttggaatt aacgttgacg agcagaaatc gaaatgcaga agaacctatt cctatgtgcg
     4981 gtgtacctca ccatgcagca caaggatata tcgatgcctt gattgaaaaa ggctataaag
     5041 tagcgatttg cgagcaggta gaagatccaa agacgactaa agggatggtc aagcgcgaag
     5101 tgatccagct agtcactcct ggtacagtca tggacagcaa aggattgtcg gcaaaagaca
     5161 acaatttttt aacagctgta gttagtcaag gaaatgaatt tggctttgct tatgccgatt
     5221 taagtacggg agaattgaag acggctcggt tgcatgatga agaagctgtt ttaaatgaag
     5281 cctctgcatt gcagacaaaa gaactcgtat taggcagtga gatcactgat acccttcgtg
     5341 aacagctaag cgggcgttta gggctagtct tttctcaaca aaatgagatg gaagaaaacg
     5401 cggaatttag ctttttgaca agcgaactag tcgatccgct ggaaaaagaa gcaaccggca
     5461 aactattgac ttatttatcg gtgacacaaa agcggagttt agctcatatc caaaaagcag
     5521 tagaatacca accagatcat tttttgaaaa tggattatta ttcaaaattc aacctagaat
     5581 taagccaatc tattcggact gggaaaaagc acggcactct tctatggttg ttagatgaaa
     5641 caaaaacagc aatgggtggc cgtttactaa aacaatggct agaccgtccg cttatccagg
     5701 caaaacaaat taaagcaaga caagaaatgg tcgcttcttt gctagacagc tattttgaac
     5761 gtatcgattt gcagtcagca ttaacgaaag tctatgatct ggaacgccta gcaggtcgag
     5821 tagctttcgg taatgtcaat gggcgagact tgatccagct gaaaacttct ttagaacaag
     5881 tgccaatgat cagagggttg attgaaggaa tcgatcaagg tcaatggcaa agtctgcttt
     5941 cagaaatgca gccgatggac catctcgtcc aattgatcga ccaagctatc caagatgatc
     6001 cgccgttaca aataacggaa ggaaacatca tcaaagatgg attcaatgag cagctcgata
     6061 tgtaccgttc tgctatgaga aacggaaaga aatggttggc agaacttgaa gcaaaagagc
     6121 gtcaagaaac aggaatcaag aacttaaaaa tcggatttaa ccgcgttttc ggttattata
     6181 tagaaattac aaaagcaaat cttggaaatg cagaactgga gaagtatgaa cgaaaacaaa
     6241 ctttagcaaa tgcagagcgt ttcatcacac cagaactaaa agaattggag acacaaatcc
     6301 tcgaagcaga agaaaaatct gttgatttag aatatcagtt gtttttagct gtgagagaag
     6361 aagtcaaaaa ggctatccag ccgctacaag tacttgcaaa agcaatcagt gcagtagatg
     6421 tgctacaaag ttttgcaacg atcagcgaac gctatcagta tgttcggcca gaacttgtca
     6481 gtgacaagca tcagcttctg atcaaagacg gacgacatcc tgtagtagaa aaagtattag
     6541 gacatcagga atatatccct aacagcgtag aaatggcaga agacgaaatg atcttgctga
     6601 ttacaggacc gaatatgtct ggtaaaagta cgtatatgcg acagttggca ttaactgttc
     6661 tgatggcgca aatgggctgt ttcgttccgg caaaacaagc agttttacct atttttgatc
     6721 ggatttttac ccgaattggt gcaagtgacg acttaattgc tggacaaagt acttttatgg
     6781 tagagatgat ggaagccaat caagccctgc gttatgctac gccaaacagt ttgattttgt
     6841 ttgacgaact agggcgagga acagcaacct atgacggaat ggcgttggcc caagcaatca
     6901 tcgaatatat ccatcgtcat gtacaagcaa aaacactctt ttctactcat tatcatgaac
     6961 tgacggtact tgagaaagaa cttccgcagc ttaaaaatgt ccatgttgga gccgttgaaa
     7021 aagacggaga agttgtcttc ttgcataaaa tgatggatgg acctgcggat aaaagctacg
     7081 gcatacatgt ggcaaagatc gcaggattgc cttccaatct actagaacga gcagcagata
     7141 tcctgcatac gctagaaaat ggcgataacg gacaccatac tgttccagct gaagtagaag
     7201 agaaaactaa aacagccaaa caggagcaaa taaaaccacc tgcagaaaaa gtaaatcaag
     7261 accatataca agaccgtgta aaagaagaaa accaacagct ttctttattc agtgaattat
     7321 ccgaaaatga aacagaagtc attgaatcac tgaagcaaat aaatttacta gaaatgacgc
     7381 caatggatgc actaaacaca ttatatgaat tacaaaaaca actgtaggaa gaaggcggat
     7441 atcgatggcc aaaatccaag aattatctga acgtttagct aatcagatcg ctgcgggaga
     7501 agtagtggag cgaccagctt ccgttgtgaa agaattagtg gaaaatgcaa ttgacgccgg
     7561 cagtactcag attgatatct tagttgaaga agcgggactg cggacgatcc aagtgattga
     7621 taacggagaa ggcattttgg cagacgacgt agagaatgcc ttcaagcggc atgcgacaag
     7681 taagatccat aatcgcgacg atctttttcg catacgaacg ctgggctttc gaggggaagc
     7741 tttgccaagt attgcctctg tttctgaaat gactctggaa acggcgaatc aggaagaaaa
     7801 acaaggcacc tatctgtcgc tgaaaggcgg agaagttatt gagcatcgac cagcaccact
     7861 gcgtcaagga acgaagatta ccgtcagcaa tctgtttttc aatacgccag cccgcttgaa
     7921 atacgtgaaa acactgcaca cagaattagc gaacatcggg gacatcgtca atcgactggc
     7981 attgagtcat ccatcgattg cttttcgttt agtacatgat ggaaacaaaa tgctggcaac
     8041 tgctggaaat ggcgatctga aacaaacaat tgcgggaatc tacggattat ctaccgcaaa
     8101 aaaaatgctg aaaatcgaag gaaaagatct agattttgag ttatttggct atgtttcatt
     8161 gcctgaggtg actcgtgcca gtaggaatta cttatcgacg atcatcaatg gacgttttat
     8221 caagaacttt tccttgaata aagcaatcgt tgcaggttat gggtcaaaat taatggtcgg
     8281 tcgtttccct attgctgtat tggaaatcaa aatggacccg cttttagtag atgtaaatgt
     8341 tcatccgacg aagcaggaag tccggctgtc gaaggaaaaa gaactaacag agctaattag
     8401 taacgccatt caaaaagcat tgtctcaaga aaatctgatt ccaagtgcag cggataatct
     8461 acggtttaag aaaaaagtac ctgatcagcc taaaacagaa caaatcgaaa tcgatttgtc
     8521 ctacgctttt gaagaagaaa aacctaaaaa gccaagtagt ttgaattttg atcgagagac
     8581 aggtaccttt ttcgtagaag aactaaagaa cacatttgtt gacagaattg ttgataagtc
     8641 tgtggaaaat gttgaaaact attctggttt ttctgaagaa aacaccactt atccagaaaa
     8701 aacagcgaaa acggtggata agtttggtga aaatgttgat aactttgtag aaaaaacagt
     8761 ggaaaaatct gaaaagaaag aatttcccat tgtggataac atggagaaaa caaatgaaga
     8821 acaagcagat aaatccgtag aagaggatga aaagatttac gaggaagaat tgacactcca
     8881 tcctgaattt gatttcactc aaggaaacga caaaacatta gaacaagcca tcaagaaatt
     8941 agaaacggaa aaaccagtac aacgttttcc tcagttggaa tattttggac agatgcacgg
     9001 aacgtatctt tttgcccaaa gtagcgatgg gttgtacatc attgatcagc atgctgccca
     9061 agagcgcatt aagtatgaat atttccggga aaaaattggg gaagtatctg atgatctgca
     9121 agaattgctt gttccatttg tgttagacta tccaaacagt gatgctttga aattaaaaga
     9181 gcaaaaacac ttgctagaag atgcagggat ttatttagaa gaatttggtc aaaacagctt
     9241 tattgtacgg gcccatccta cttggtatcc caatggagaa gaagaatcga ttattcggga
     9301 gatgatcgat atgctgctga caactggaag tgtgagtgta aaaaaattcc gtgaagcaac
     9361 agcaatcatg atgagttgca aacgatcgat taaagccaac cattatttga atgaagcaca
     9421 agcacgtgtg ctgctgaagg atctggcacg atgcgaaaat cctttcaatt gtccccatgg
     9481 ccgtccagta ttgattcact ttaccaattc ggatatggaa aggatgttca aacgaatcca
     9541 agacccgcat taagtcaaag agaagaggga aaacatgaag attgtattag catcccaatc
     9601 accaagaaga aaagagttat tggctcatat cgtttcagaa ttcgagattg aacctgctga
     9661 tatcgatgaa acgccatttc cagacgaaca gcccgtcgat tatgttcgta gaatggctga
     9721 agaaaaagca cgaactgtct gggaacaaga tgaaaaagaa gaccatttag tgattgccag
     9781 tgacacgact gttgttctca atcaagagat catgggtaaa ccagaagatc tggctgaggc
     9841 tgaattcatg ctgaaaaaac taagcgggga aacacatact gtatatacag ctgttgtttt
     9901 aaaaacaaag gaacgagaag aacggatact agcagaagca catgtcactt tttatccttt
     9961 gacagacgaa gagatcaaac gctacttgga gacgggggat tatgcggata aggcaggggc
    10021 atacggcatc caaaatgctg cgagtgtatt cgtcaaagaa atttctggag attattacag
    10081 tatcgtagga tttccgattg gtgctgtcaa tcaggcactg aaaacattca tttaaacaaa
    10141 aaagcggact atctgatttt cgattgaaaa tccatgatag cccgtttttt ttattgaaaa
    10201 gattacttat ctactctgac taagatttta cttcagcttt accgactgct tcgcccgggc
    10261 gaacaagtcc tgattggtca agagaaagtc ctgcaacttt ttcattattg gtcaaagcaa
    10321 caataatgtc agttttcttg ccagcttgtt ctatttctgg caaatccatt tcagcaatca
    10381 gggtctctgg tgtgacaagg tatccttctt tcacgaatac attgaaagga acacccttta
    10441 gttcaacagt atctaatccc atgtgaacaa gtacttccag cccctcatta gaaagaatac
    10501 caatggcatg ttttgtttca aagacactcg tcactttacc gtttactggt gcatagactt
    10561 tgccgtttga cggttcgaca gcataaccgt cacccatcat tttttgtgag aaaactgggt
    10621 catttacttc agttatttcc ttgatggtcc ctgcagcgac tgcgaataat gtttcttttg
    10681 ttgcaactgc cgtaccagtt gctgaaacag gttcgaaaga ctgtggcgtt gctgttgcgc
    10741 catttgctgt ttgaagttgt acatcagcag cattttcagg aacagcagga tcaatcttat
    10801 tcaaaattcc ctgacgtcta aagatgatcg tcaagccaaa tggaatgatg acagcaatga
    10861 tcattgcgat gatgaaaggt acccatgttt ctgcttggat agctagtatt cctggcaaac
    10921 cgcctactcc gatcgcgtta gctcgaacac ccatcaagtt agcaaacatg ccagctaatc
    10981 cagagccgac cattgcagcg acgaaaggat agacgtattt caaattgatt ccgaacatag
    11041 caggttcagt tacgcctaag taacaagaaa tcattgctgg aatcgagatt tgttcttctt
    11101 ttttattccc acgatgtaag aagatgatgg ctaagactgc agagccttgg gcgatatttg
    11161 ataaagcaat catcggccat aaattcgttg atccgaaatc tgcaatcagc tgcatatcga
    11221 tggcattggt catatggtgg agaccagtaa tcactaaagg agcatacagg aatccgaata
    11281 cggcactaaa caaccagctg atagaagacg tcaatccagt atttacgata tttgagatcc
    11341 agcttccgac tgtccagccg ataggaccta aaataacgtg tgcagctaaa acagttggta
    11401 acaatgaaaa caatggaaca aaaatcatcg agatcgactg agggatatat ttgcggaaga
    11461 agatttctag atacgctaac ataaaccctg ccagcatggc tggaataact tgcgcttgat
    11521 aaccaatcat ctggacttga gcaaaaccaa aatcccaaaa tggaatatct gctgcggctg
    11581 tggaagctac actataagca tttaataatt gcggagaaac taacgtaatt cctaaaacga
    11641 ttcctaatat ttgtgtagta cccatctttt ttgcaatact ccaagtgata cctactggaa
    11701 gaaaatggaa aattgcttca cccggtaacc acaaaaaggc attgacacca ttccaaaact
    11761 tcgagtgttc aacgattgtt ccaccaagac tttcaaactg gataccttca agtacatttc
    11821 tgaatccaag gatcaatcca ccgacaatga ttgcaggaat cagtggtgta aagatttctg
    11881 ctaaaacggc aatagcacgt tgaacgggat gaaggttttg ttttgcagct gcttttcctt
    11941 gttctttgga aaccccttca actccagata ctgcaacgaa gtcgttataa aaagtagaaa
    12001 catcgtttcc tatgatgact tggaactgtc ctgcattcgt gaacatccct tttacgctag
    12061 gtatatcttc gatcgctttt tcgtccgctt ttcctggatc attcaggaca aaacgcatac
    12121 gagtcgcaca atgactaact gcagcaatat tttcttttcc gccgatatca tgtaacagct
    12181 tctcggcatc tgcttgatat tttgccatat caataaaccc ctttctctta acttgtctat
    12241 gcaagttgtt tcttcattta ctatactttt tttcggagag aattgcaagc gttttattaa
    12301 aaagtacgag agaaaatgat atcgttttaa aaagcttaaa ataagctttt cttaaaaata
    12361 ggaacattga aatacaaaaa gagctgtagt atagtgaaga aatgatggaa gaacgagtga
    12421 gggaaaaaac atgaataagt tcaatgaaat ttttttagat ttggaacaga aaattttagc
    12481 gaaagaatat cctccgcatt cgcttttacc cagtgagaac caattgatca aaatttatgg
    12541 tgtttcacga gaaacaatta gaaaagcgct gaatcttttg actacggaag gttatattca
    12601 aaaaaaacaa ggaaaaggtt cgatcgtact ggatcggaat cgcttcgact ttcctatttc
    12661 tggattgact agctataaag agctacagga gacccaacgt attccaagtg aaacgatcat
    12721 ccatacgtta gaagaaacag aagtgacaaa agcattgaac gagatcactg gctgggagat
    12781 cggtgcgcct gtttggcatt tgatacgcga aagaaaaatc aatggcgaag tagttatatt
    12841 agacaccgat tatctattaa aggaaatcgt tcctatttta actccagcac aagcacaagg
    12901 atcgatctat gaatattttg aaaatgagct ttctttgacg attgcttacg cgcaaaagga
    12961 gatcacagta gaagaagtaa cggaaatcat gaaaacagcg atggatcttc acgatgacac
    13021 ttatgcagta gtagtcagaa gcctcgttca tctggaagat acccgctgtt tcgagtattc
    13081 tgagtcggtt caccgattag ataaattccg tttcgttgag tttgcccgac gccgaaaagt
    13141 gtaaatagtt caaaagaaag caaattgttg gcatctgtcc actttggcgc tatgatagga
    13201 tcacaaaggg ggcattttta atggataaac caataagacc agaaaaagaa gaactgaaag
    13261 aaaagttatc acccatggct tatgcagtca cacaagaaaa tgcaacagaa cgaccattta
    13321 gcgggaaata tgatgatttt tacgaaaagg ggatctatgt agatatcgtc agcggtgaac
    13381 cgttattcag ctctgcagaa aaatatgatg caggttgtgg ctggccatca ttcagcaaac
    13441 caataacgaa aagacaagtg cgcgaaaaag ccgatttttc tcatggcatg catcgaatag
    13501 aagttcgaag caaagaagca gattctcatc tcggacatgt gtttaccgat ggaccagtag
    13561 atcaaggtgg tctacgttac tgcatcaatt ctgcctcact gaaattcatt ccatatgaaa
    13621 agatggatga attaggttat ggggaattca aatcacttgt agaataatgg aaatacacaa
    13681 agagcggtac aatacgggga tttactcgat cgtccgctct ttttttattc aagcgattgc
    13741 tatttgaaag aaacgctccc tagactcctt ttttcacaag gtgaaatgtg ctacaataaa
    13801 gggaacgtat gtgtgcaaaa gaaagaggac tagaatatgt atgaatatct gacaggtgtg
    13861 gttaccttta tcaaccccta ttacatagta gtagaaacca atggtatcgg ctaccagatc
    13921 gcactaggaa atccttaccg ctacagtagc aaactaaatc aaaaaataaa aatttatgtc
    13981 catcaggtaa tacgtgaaga tgcgcatctt ctgtatggat ttgattcgct agaagaaaag
    14041 caactgtttt tgcgattagt aagtgtttca ggaatcggtc cgaaaagtgc ccttgcaatc
    14101 atggcaagcg aagaccactc cggtttgatt caagcagtag aatccagtga tgtcacttat
    14161 ttgacgaaat ttccaggagt agggaaaaag acagcacaac aaatgatctt ggatctaaaa
    14221 ggaaaattcg gtgagctgag tatcgataca ccttttagtc tatttgatga agcaaacaca
    14281 aaagacgcta cagctctatc tgaagcaatg gaagctctct cggctcttgg ttacagtgat
    14341 cgagaaatca aacgtgtaga aaaacaatta aaagaagtag ataatcagac aacggatgag
    14401 tatctacgcc aagcactcaa gctaatgatg aaaaaataag gaggagacca aatgacagat
    14461 gaatacttgt tatcaccaga atctggcgaa aatgaactga gtttggaaaa atctcttcga
    14521 ccacagctgc tttctcaata tatcggacag agaaaagtca aacaagaact tcagatttac
    14581 atcgaagctg ccagaaatcg ggaagaacct ttagatcatg ttctgttgta cgggcctcct
    14641 ggattaggaa aaacgacgat ggccatggtc attgctaatg agatgaccgt aaatattcgt
    14701 acgacgagtg gccctgctat cgaacgtccg ggtgacttag tagcaatctt gaatgaactg
    14761 gaagcagggg atgtcctgtt tatcgatgag atccatcgat tacctagagt ggtagaagag
    14821 atgctttatt cagctatgga agatttctat gtagatatca tggtaggaca agggccaacc
    14881 gcacatccgg ttcatttccc tcttcctccc tttactttga ttggcgcgac gacacgggca
    14941 ggtatgcttt ctgcaccttt acgagatcga tttggcatca tttctcatat ggaatattat
    15001 gacgaacagg atctgcagga gatcgtttta cgttcttcgg atatttttca aacagagatc
    15061 attgaagaag gggcattgga aattgctcgc cgatcacgag gaacgcctag aatcgctaat
    15121 cgcttgctca aacgggttcg tgattttgcg caagtccaag gagacgggaa aatcgatcgt
    15181 cctatagcgg atcaggcact tactttgcta caggtcgatc aggctggatt ggattatgtt
    15241 gaccagaaat tgttgcgaac aatgatcgag ttatatggcg gcggaccagt agggttaagt
    15301 acgctttcag taaacatagg agaagagcgt gagacggtag aagatatgta tgaaccgtat
    15361 ctgatccaaa aaggattttt aaagcgtaca cctagagggc gaattgctac tgcttatgcc
    15421 tatgaacatt ttggttacga ctatcatata tgatttttgc ttttagattg gaggaaaatg
    15481 aatgagtgat acaacacgta cgaaacaatt gatcaaaaac gtattgattg atttgtgcca
    15541 aacgcaatct tttcgaaaaa tcagcgtcca aaaaatcgca aacgaagcaa aaatcaatcg
    15601 acaaacattt tattatcatt ttacagataa atacgatttg cttcgttggg tctattgtga
    15661 ggatgcattg cattatttag atccacaatg tctttcactt gataattggg aagagcaagc
    15721 attgaaaatg ctgaaagcaa ttgctgagaa gaaagaattt tatgcaaata ctgtgctttc
    15781 tgacccagaa attttacaaa aagagttttc tcaactgatc caaaaatcat ttatcaagat
    15841 ttttgaacaa atggatgaag aaagacaact ttctgcaaga gacaaactat tttatgctcg
    15901 ttttttttct tacggttgca gtggtgtatt agtcaattgg atcacgaatg aatatgaaga
    15961 aacaccgatg gaaatcgcta tgcagctttt ccgtttagcc aaggatacgg aattatattc
    16021 ttatcgtctt tatgcaaaag aagagttgga agaacagtaa tagaaaaatt ttttgttgga
    16081 aaagcaataa tcgctaaaag gagatgggga aatgacaaaa attttatttg tttgtttggg
    16141 aaatatttgc cgttcaccga tggcagaagg cttgctgaga aaacgaatag cattagaagg
    16201 aagggaagca gaattttttg tggattctgc tgctacaagc acgtatgaag ttggaaagac
    16261 accgcaccct ggaacgaaaa aaatattaaa ccaagaaaaa gtagatatga cgaatatggt
    16321 tgctcggcaa atcacacccc acgattttga aacttttgat tggatcattg gcatggatca
    16381 agaaaatgta gaagaactga aacgaagagc accacgctca gcgcagggga aaatccattt
    16441 gtttttaagt agcattcctg gaaaagaaaa agaaaatgtt cccgacccat actataccaa
    16501 taactttgat gagacatatc acttgatcac agaaggcctg aatcattggt ggaatatttg
    16561 gattgatgaa aataaaataa caaataattt gtaaagttaa cgctttcttt ttaaggcgat
    16621 gttagagcat ttccttgttt ttttgtaaaa tgttaggtaa aataaaatta attaagtaac
    16681 actttcttag ctaaaggagg agtgcattcg atgaacgaag agacagtatc gccaatgaag
    16741 aagcttacga agttagttac tcatacttcg tatcaacttt ttgattcttt tgtaaatgac
    16801 actgaagaac atgctaagaa tgtacgattg caaggaaagt ttattcttga gctgcaaaaa
    16861 gctgctactg aacatagtct agttatctta caagtaagag caaatcgttt gagtagcaag
    16921 tatgaaacga tctcaggctg ggtggcgaca aaaacggtcg aaaaagaccg agtgatgatc
    16981 cgcctacaaa acgaagagca gcagatccgg atggttcctg tagaacaaat tgaaaaaata
    17041 acaatccttt caccagcagg ggaacaggaa cggttatcaa aataatttat accagataga
    17101 aaagcacgta tgagcttgta ccatcaatca ttactaatca aaaataaccg aatgctttaa
    17161 gctgatatca gtaaaacgga acgttttagg atgagtatca tagcatgaca gcattcggtt
    17221 gattagttga atcgattgga tggtacaagt ttttttgata aaaaaaggtc ctgctagccg
    17281 gggagctagc aggataagga gttaaaaatg aaaaagtgtt gttagggttg tttgttggta
    17341 tgcttatata atagtcatta gttgtgaata ttttgtgacg aaagttttcg aaagttgtta
    17401 agtt
//
LOCUS       DACEZN010000049        17366 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEZN010000049 DACEZN010000000
VERSION     DACEZN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.049"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(476..1677)
                     /locus_tag="IXH60_001266"
                     /pseudo
     CDS             complement(476..1677)
                     /locus_tag="IXH60_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1794..2309
                     /locus_tag="IXH60_001267"
     CDS             1794..2309
                     /locus_tag="IXH60_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1990205.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2508..2954)
                     /locus_tag="IXH60_001268"
     CDS             complement(2508..2954)
                     /locus_tag="IXH60_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1990206.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3001..4479)
                     /gene="msr"
                     /locus_tag="IXH60_001269"
     CDS             complement(3001..4479)
                     /gene="msr"
                     /locus_tag="IXH60_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1990207.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4833..5462)
                     /locus_tag="IXH60_001270"
     CDS             complement(4833..5462)
                     /locus_tag="IXH60_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1990208.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5446..5910)
                     /locus_tag="IXH60_001271"
     CDS             complement(5446..5910)
                     /locus_tag="IXH60_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1990209.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(6397..7665)
                     /locus_tag="IXH60_001272"
     CDS             complement(6397..7665)
                     /locus_tag="IXH60_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1990210.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7896..8633)
                     /locus_tag="IXH60_001273"
     CDS             complement(7896..8633)
                     /locus_tag="IXH60_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1990211.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8700..9269)
                     /locus_tag="IXH60_001274"
     CDS             complement(8700..9269)
                     /locus_tag="IXH60_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1990212.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            9397..10269
                     /locus_tag="IXH60_001275"
     CDS             9397..10269
                     /locus_tag="IXH60_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1990213.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10611..11483
                     /locus_tag="IXH60_001276"
     CDS             10611..11483
                     /locus_tag="IXH60_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1990214.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11480..13438
                     /locus_tag="IXH60_001277"
     CDS             11480..13438
                     /locus_tag="IXH60_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1990215.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13689..14540)
                     /locus_tag="IXH60_001278"
     CDS             complement(13689..14540)
                     /locus_tag="IXH60_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1990216.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14663..15130
                     /locus_tag="IXH60_001279"
     CDS             14663..15130
                     /locus_tag="IXH60_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1990217.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(15393..15875)
                     /locus_tag="IXH60_001280"
     CDS             complement(15393..15875)
                     /locus_tag="IXH60_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1990218.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15975..16838)
                     /locus_tag="IXH60_001281"
     CDS             complement(15975..16838)
                     /locus_tag="IXH60_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1990219.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         5213 a         3594 c         3066 g         5493 t
ORIGIN      
        1 gcaattcttt gatatagcca cgataaatca accgatcatc tgaaaattca atgggtaatt
       61 caacgagtgt caacccgact tcctgaaaat attcttccag catctattat ccccccattc
      121 tttttcttaa atgaatttta gcttacctac tgaaaatttt ccagtttcat tattttgaga
      181 aaaaaaggga atgtgacaaa aatcgtgtca catccccttt ttccgaataa atggtgttct
      241 tctgccaact cctcaatcat aagtcacaat attcaacaaa catgagaaac tgttttttga
      301 aattgttcct tgtgattcag agttaaacga cagtctcaca acctcttggt aaacggcgtt
      361 caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgaga agctattttt
      421 gtttggctgt tcttatgact tgatgcagga cagctttttc acaacctctg atttcttatt
      481 cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca ctgataagaa
      541 caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca atcacacctg
      601 caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg gacgaagcaa
      661 cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta ttgataattc
      721 cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata gaagttccag
      781 ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt gtccgataag
      841 tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc agaagcacac
      901 cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat aaaaatggcg
      961 catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt ccaactgaac
     1021 gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt ctctttggtt
     1081 tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc aaaaatgcta
     1141 ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact agagggccaa
     1201 cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct gtgttaccaa
     1261 ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc ccgacacccc
     1321 agccggcacg taacccaatc agcattccga tattcccaga taatccgcca agaccagcga
     1381 atacaacgat gataaacaat cctgtcatca atgttttttc aaaccgatcc gactagataa
     1441 gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt
     1501 cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag
     1561 acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc
     1621 ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc
     1681 tttctctcta ttagcatcat acatgattta ttttacatgt aaatatgcac atgtaaaacg
     1741 tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata
     1801 ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga
     1861 agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca
     1921 agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga
     1981 tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc
     2041 ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata
     2101 aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa
     2161 aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg
     2221 ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa
     2281 gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta
     2341 ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg
     2401 aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac
     2461 tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt
     2521 tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa
     2581 gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt
     2641 ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag
     2701 catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca
     2761 atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca
     2821 ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac
     2881 gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt
     2941 ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat
     3001 ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc
     3061 agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat
     3121 ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc
     3181 taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc
     3241 tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt
     3301 cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat
     3361 ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg
     3421 agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga
     3481 accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac
     3541 cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct
     3601 gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt
     3661 tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt
     3721 ttgtacactg tctttttgtt tagaggaaga caaccgatcc ggtctgattt gttgcttttt
     3781 ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt
     3841 ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc
     3901 caattccttt tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc
     3961 ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa
     4021 aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc
     4081 tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat
     4141 caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct
     4201 taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa
     4261 ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc
     4321 accggctatt agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat
     4381 acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct
     4441 gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac tcctatttca
     4501 tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg
     4561 catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata
     4621 gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg
     4681 tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat
     4741 cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa
     4801 gcaatagcta ctgaatagta actgaaaacc ttttattcga acgtagtgat cttttctggt
     4861 gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa aataataact
     4921 ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca
     4981 ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct
     5041 aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa
     5101 ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa
     5161 gcttggttat agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc
     5221 attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct
     5281 tttgcttcct cgctttcagc gaccacaaag cgccatggtt gcatattgac agaagaaggt
     5341 gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca
     5401 tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc
     5461 attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc
     5521 taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc
     5581 tgtatcttta tggtccattt cacaatgctc gattttcttc tgggcttttt ctgttagcgc
     5641 tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc
     5701 caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact
     5761 gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact
     5821 tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata
     5881 taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc
     5941 aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta
     6001 ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg
     6061 gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat
     6121 agaagatagc tcttctgcag tagaaaattt tacaccgcca aaaatttccg aaatcgcctt
     6181 ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa
     6241 gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct
     6301 gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta
     6361 ctacttgatg cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc
     6421 ttcatatctc cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg
     6481 atgggtacca gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga
     6541 ataaaaactt agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg
     6601 tgccattgtg taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc
     6661 gctgtttggt gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt
     6721 tttttctccg gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata
     6781 catcggagaa gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac
     6841 attgttcaat gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt
     6901 agcatctttg taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg
     6961 ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg
     7021 atattttcta aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg
     7081 catcgtaaca gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc
     7141 agaagccaaa gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga
     7201 tgcatataag tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa
     7261 cgcttgttga gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa
     7321 ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt
     7381 gatttcttct gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg
     7441 tgtttgttct gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagacagt
     7501 agctgactgc aaagtactct ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt
     7561 ctgagttgcg acagtttctg tcgattcttc agctagcact gttgtgttgg caactgctag
     7621 atttcctaaa agaataacgc taaataataa attctttgct ttcatctttt ttctcctttg
     7681 aattatgaaa cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc
     7741 atatttaaat aacatttgtt tctttttgat aaatttgctt tttctacata aaaagacgta
     7801 aatgaagaat attcttctcc attcacgcct taaattgatg gtcgtttgaa taactattta
     7861 acaagtaatt ctctttggat attatccgtt atggattata cttcctgtgt cgtcggacgg
     7921 ataatcatgt tactgacaga catccggtct ggcgtttcta tcgcaaatgc tacagcagaa
     7981 gctatatctt cggaggtcaa tccccattct ttttgcgctt catgaagttc aagagcagcc
     8041 tttttgtcgc tgatggtttg atataattca gtttgaacag ctcctggaga gatgatcgta
     8101 gatttgatgt tattttgccg ttgctcttga cgtaatcctt ccatgattgc acggacagca
     8161 aattttgttc cgcaataaac ggcggaatca ggataaacaa catgtcccgc cacagaatca
     8221 gttgaaatga tatgcccaga tttttgtttt tccatgattg gaagtacggc ggaaattcca
     8281 tttaacacac ccatgatgtt gatatcaagc atattttgcc attcatcacg gtgaccttca
     8341 actaatggag cggttggcat gattcctgca ttattgtaca atacatcgat tcgaccgaac
     8401 ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa aatctcttac atctgctgtt
     8461 tgaatatata gctcagcttc tggtaaagat tcttttattg cttttaggcg atcttctcgg
     8521 cgtgcagcta taactagttt agcccctttt tctgctaata gcttcgttgt tgcttcaccg
     8581 attccgcttg aagcgcccat gatgacaatc actttttctg ataaatcatt cattctattc
     8641 tcctcctgtt ttgaaaaatc tcttgcttat actcactatc tcttatttca tcttactgtt
     8701 caaataatcc gttcgacagg tttgacgatt ttggcaggga ttcctgcaac gatggtattg
     8761 tctgggacat ctttcgtaac agttgcattc gctgcaacaa tggcattctc gccaatcgtt
     8821 acgcctggca gaatagttgc tcctgctccg atccaagcat ttttcttgat gtggattggc
     8881 tttactgaca agcctcttcg ttcttttgga tcggttagat ggttcaccgt gatgaggttg
     8941 acacgaggac caatcaatac tgaatcttct aaacagatgc cacctaagtc aacgaacatc
     9001 acttgacgat tgataaagac atttttccct atactaatat gtttgccgaa atctgtataa
     9061 aaaggcagca gaacagtcac cgtatcatct actttgtcac taatgatatt ttctagccgt
     9121 ttgcggatat ctgctggttc atggtaccca gtattcagcg ctgctaaagg ctcttcgttt
     9181 tcagcttgga tctgatggat cacttcaaag agcggatccg ttactcgaat ctctttattg
     9241 acgatttgtt tttcgatgag gcttgtcatt ttgctccctc cttttatcct tactttataa
     9301 cggacggctt atcatgtcta atacttataa aacatactta actattcctt ttaactatac
     9361 ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg atattcgtat actgacgtat
     9421 tttttagcag tagctagaga aaaaaatatc agccgagcag caaaatctct gcatatcacg
     9481 caacctactc tctcaaaaca actgaaagat ttagaagaag aacttggtgt gacacttttc
     9541 actcggggct cccgagagat acagttgacg gattctggtc gatatcttta ttcaaaagga
     9601 aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc
     9661 tctggagaaa tctacattgg cggaggcgaa actaaagcaa tctctttcat ttctgatagc
     9721 cttcattcgt tgatcaaggc gcatcctgat atccagtttc atttatatag cgggaatgca
     9781 gatgatatca tagacaaact ggatagcgga ttacttgatt ttggactcgt aatcgaaccg
     9841 acaaacaaac aaaaatacca gtcatttcga ctgccagccc aagatcgttg gggacttttg
     9901 atacatcgaa atcatccttt agcaaagcaa accgtcatcc atccgcagga actgaaaact
     9961 gttcctttga tcatttcccg gcaaacgtca gtggacagtc aactagctga atggctaggt
    10021 ttcagcatag atcagttatc gatcgtcgga acatataact tattgtacaa tgcttctttg
    10081 atggtcgata acgggacttt tggtgcatta gctctagatg ggatcatcaa tacttatggg
    10141 acggaaaaaa tattcatccc actcgatccg ccattacaag cttcactgaa tctcatttgg
    10201 aaaaaagacc agcctctttc tcctgctgct cgtaaatttt tagatgtcct aacggagaaa
    10261 tacagttaag tagagttttt ttccttcgct ttcagctttt taacaacgaa taagataagc
    10321 aaaagatcca acgattaaat aacataagtt tcttaattta tttcttcctt ttatttctga
    10381 taattccagt aaattactat aattataaaa aaagaattga caaaacataa ctgtctatat
    10441 aaaattgatt acgttaacaa taattcgttt catgtgacta gatcatacta ggcatgaaaa
    10501 atctctgagg aacatggttt ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa
    10561 tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca
    10621 aaaagatctt taatcaaaat gcagtattgg tagatgacgg tggacaggaa aaagtagcta
    10681 ttggaaaagg gattggattt gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg
    10741 aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga
    10801 ttgatgaacg ctttttattt gcagcagaaa cgatcattga tcatgcggaa actgttttga
    10861 tggaaaagtt gaatgaacat gtattgatcg ctttgaccga ccacattgct ttttctgcgg
    10921 agaacattaa taacggtatc gtcatccgca acaaactttt aagagaaatc gaagtgcttt
    10981 acagtgatga attcagtatt gctcaatggg cagtagagta tctgacaaaa gagcttggga
    11041 ttccatatac ttatgacgaa gcaggatata tcgcaatcca tattcacagc ggacgaagcg
    11101 gtcggaacaa taaccatcgc agtatccgtg aagtgacgat tgtatctgac atcatccatt
    11161 tagtggaatc agaattggaa acagatatcc attcagaacc attttcactg aattattcaa
    11221 gactggtgaa tcatttacga ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt
    11281 tagacacgga aatcgtagaa atggtcaaac gaaaataccc agaaagctat aagatttcta
    11341 aaaaaatccg tgtgttgctg accaaagaat accagctcgc gatcacgaaa gaagaactgg
    11401 gttacttggc catccatatc gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca
    11461 atcatgagga ggaaaaaaat tgaaagcata tctacaacga atgggacgtt cccttcaact
    11521 tccagttgcg gtcttacctg ctgctgcttt actagtaggg atcggaaact ggtgggcatc
    11581 ttatagtaac gacattgtcg ctcatttttt acaagctggc ggtaacgcag ttttaggtca
    11641 attacctttg ctgtttgccg ttgggttagc actagggatg tcagtggaca aatcaggtgc
    11701 tgctgccttg gcaggtttgg tcgcatttga agttccagta aatgttttaa aaactgattc
    11761 agtagctact ttattgaata tcaaaccaga atcagttgat ccatcttttg ctcagatcag
    11821 taacgtattt attggtatta ttgccggatt gattgcggca gctctttata atcgtttcca
    11881 cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc aagcgtctag tgccaatctt
    11941 ggctgccttt gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc ctccggttta
    12001 ctctgcttta gtttcttttg ctaaattcat tttgagttta ggctggggtg gtgcaggtct
    12061 ttatggcttc ttcaaccgtt tactgatccc tacaggattg catcaagcaa tgaactccgt
    12121 gttctggttt gacgtagcag gaatcaatga tattggtaaa tttttggctt ctgaaggaac
    12181 aaaaggcgta acaggaatgt atcaagctgg atttttccca atcatgatgt tcgggcttcc
    12241 agcaggagca tatgctattt acagaaatgc acgtccagaa aacaaagcaa aaacagcttc
    12301 cttgatgcta gcagcagctt ttgcttcatt ctttactggt gtgacagaac cacttgaatt
    12361 ttcatttatg ttcgttgctt ggccattata tgtcttgcat gcaattttca ctgggatttc
    12421 tttagcagtg agtgcattct tccattggac agcaggattt gcctttagtg ccggatttgt
    12481 cgattacttc ctatctttga aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca
    12541 aggattagta acagcagtga tttactactt tggctttaac tttgctatca aaaaattcca
    12601 tctgatgact cctggccgcg aggaagcaga tctttcagat gacgatactg caacgactaa
    12661 ttcagacaat aaatatgcgg cacaagcaag caaaatctac gctgcattag gcggagccga
    12721 caatgtaact tcgatcgata actgtactac acgccttcgt ttgcaagtca aagatacagg
    12781 cctgatcgat caaaataaaa tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg
    12841 aaaaaatgcg caagtcatcg taggtacaga agtacaattc gttgcagatg agatggcaaa
    12901 actacatggt ggtgctgcag cacgacctgc acaaacaaac accgtggtta aaacagaaac
    12961 atttgccggt gaaacgatcg aacaagatat ttacgctatc gcaaatggga aactgattcc
    13021 aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt
    13081 attaccagaa aatggagaaa tcttctctcc aatcgctggt acgatcacaa acattttccc
    13141 aacgaaacat gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc ttcatatggg
    13201 catcaacact gttgacttaa aaggcgaacc attcacttta tatgtagaag aaggtcaaaa
    13261 agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa
    13321 aaacacggat atggtcgttg tattcacaaa tggcgacaaa gtccaaagct tagaaatcga
    13381 acccgcacgt gatgtaaaag caaatgataa aatcggctct gtttccaaca aagcctgagc
    13441 catcgtcttc cccagacttt tttatcgata aacaaacagg agctgagaca aaaatcttgt
    13501 ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg taataagtcc
    13561 agctaaacga aacatatgaa gagctatttt ttatttgact cttcttatta cttgatgcag
    13621 aacagctttt tcacaaaatt tttcttcata ctcaaaacta atcggctttt tcacaacctc
    13681 tttatacatt attcctgatc caatatttct tttaatgctg cttcaagaga agttaatggt
    13741 tttcccaata ctttttctaa atcatcagag gaaatagcta actgatcctt tttaatatca
    13801 tgttggatcg ctaaaaacat gtcagctgct tcttgaggga atccggcttc ttttagattt
    13861 tcgacaaacg ctgcatcact tgcttcaata atttctaact ctttacctgt cgcacgctct
    13921 aatgccttgg ctaattcttc atagtcagtt agattgccgg ataattctaa gatttctgga
    13981 gagtcacttt ctactagtac ttttgctgca gcttctgcat attcacgttt caatgcccaa
    14041 cctgtttttc catttcctgc agcataaacg aattgaccag tttttaaagc ctgcccaatg
    14101 ataggtaatt cattctctac ataccaatta ttccgtaaaa atgtatggga aatacctgaa
    14161 tccttaatca ttttttcagt gaattggtga tctggcgcta atacactgat cgagtgatcg
    14221 gcatctgcaa aacttgtata agcgatataa gataccccag ctgcttgtgc tgccttcact
    14281 acattcttat gttcggcttg acgatttcct ggaacgccag agacaaaaag caatcgatct
    14341 attcctttca acgcttcttt cattgagtct aaatcatcat aatcaccaat acgtatatta
    14401 aatccagctt ctttcagtgc ttctcctttt tcttcactac gtactagtac atagatttca
    14461 ttttgtggaa ccatcttttt taagaattcc agcgcataac caccaaaacc tccagttgct
    14521 cctgttacta aataatgcat atttgccact ccctattctt tttatttgtt aagatttaca
    14581 gctgtacatt tataaattac acctttatca taaagagtgt ggtaagataa gtcaagcaaa
    14641 aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa tttagtgatg caatccatgt
    14701 acttgcctat attgaaatgt ttcaagatac ccgatcactt tctagtgaga tgattgccag
    14761 tagtatcgaa acaaatgcag caaatgtacg taaaatcatg agtcagctca aaaaatccgg
    14821 tttgatcttt acaaaaaccg gaaaaccgca gccttccctt gccaaaagtc cagatcagat
    14881 ttctttacta gatgtctatc gcagcatcga aggtaataca cagcttatcc aagtcgatcc
    14941 aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa gaagctttag aagaaagcta
    15001 cgctcggctg cagaaaaagg cagaagaaga aatggctaag atcacacttg atagtctgat
    15061 acataaaata gccgttttgg aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag
    15121 atttttataa ttctctctac tgtgcctgca tttttctgat cggaatcgtc tacataaatt
    15181 cttttattct ccgggggaca aaagccttgt catgcccctt tttcctctct aatgctcagg
    15241 tcaaagcgct gaagtcatga cgtcttgata aacggtgttc aagtagctga ccctcggcat
    15301 tagttctgaa aaatgcaaaa tatggaaagc tatttcccat ttttcctctc taatgctcag
    15361 gtcaaagcgc tactttcacg acctctatat tatcatggtt tattgactac cggtatccag
    15421 atttctccca tatatttccc gttggtaact cccatttcga ctacagcatt tggaccccca
    15481 acataggcgt aatcttctac ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca
    15541 tttcttaatt cttcaggcga ttctgctaaa gcgcttacaa caagatacgt tccttttggg
    15601 aatgggatga ttcgcgtagt gtttggaaca tcctgactcg tttgaacacc gacatagtac
    15661 atcattttcc cgttataggg ttcgttgact gtaaagaaat aatgattttc agcagcttgt
    15721 ttcaatgcat ctattgttcc attaccgata actttttgct caaatgcttg tttctctgca
    15781 gccaaaccga caaaatccgt attgctactc ttcaattcca caccaatacc cgtcaccgta
    15841 aaatctggtt tttcttcgat cctatacttt gccatttctc ccaccctttc tattatatta
    15901 agttcatctt acataaaatt aaagggaaat gacacgatta cgctcttttt tctagggata
    15961 tttttatttt ttaactattt ttttgctttc actgcaatcc aaatctcact gtaaacatta
    16021 ctgaaatcct ctccccattt tgtaaaagag atctcgggag cttcgactaa cccataatct
    16081 gaagaaggaa gccattctgc atagattttc ccccaagtat cttgtaaagt ttggggaaat
    16141 ggccctttat taggaaaaat ggcccaagtg tgttcttcta tagcaagcat ttctaaatca
    16201 gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt gcatcaaact gcccttttct
    16261 tccaagcggt ctccatcaaa tccataagaa acgttgatta cttgatttgg atataaatct
    16321 cctaacttat gcatctcttc cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt
    16381 tcttgattga ctccttcaaa tttgacagga acttttttcg ccacacctgc cacataaaac
    16441 tgttctttct tttcgatacg aaattccatc gattttcctc ctctgacatc aataaaaaat
    16501 gtcaagcgtg gaaaagattt ttgtatgccg tatctactga tttctgaagg cagatatcct
    16561 gaccaatcac gaaaagctct tgaaaaacct tcgactgttt catatccata tctaaacgcc
    16621 gtttctgtca ctcttgatcc atctagaaga tcgcgattag ccaaagccaa tctacggttt
    16681 ttgatatagt ccattagcga catccctgca ataaacgtaa agatgcgttt taattgatat
    16741 tcagagattc ctgtattttc ggctattatt ttataggaaa gtccttctgt caaatgttct
    16801 tcgatgtaat cgatcaattg gttcagatct ttaagcatat ttatcttcct ccttgattgc
    16861 ctttttatca tattatcaaa gaaagtcaat tacttgatga atcaggaacc aatttagtca
    16921 gatgtagaaa atgttgtatg gatctaaaga aaattctcaa gcagcgtttc gtaacggata
    16981 attggtatta tctgcctatc tatgttttac ctttagaaaa ataaattttg gggaagacag
    17041 attctaagta aacaaagcgg taaaaatatc ttttattccc ttagttaatc cgcttactac
    17101 ctactgtaca aagcaaattg ttgttcaaac tgctgatcag aacgctgctt ggatttttct
    17161 ttgcgataca tcatacctct tttatcaatt ggaacaatta tttatttcta acaaaatcac
    17221 ttcatttttg tcagataaat aaaaaacaaa aaaagttcca tctttaaaac acgctattct
    17281 tttacttttt ctcacattct ctaagaaaag aaaaggagaa tgaatttttg acaattcgag
    17341 caaaagtgat tatcaaatat gtaaaa
//
LOCUS       DACEZN010000050        17267 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEZN010000050 DACEZN010000000
VERSION     DACEZN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.050"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(94..969)
                     /locus_tag="IXH60_001282"
     CDS             complement(94..969)
                     /locus_tag="IXH60_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1990220.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1051..1842)
                     /gene="codY"
                     /locus_tag="IXH60_001283"
     CDS             complement(1051..1842)
                     /gene="codY"
                     /locus_tag="IXH60_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1990221.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="IXH60_001284"
     CDS             complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="IXH60_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1990222.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="IXH60_001285"
     CDS             complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="IXH60_001285"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1990223.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="IXH60_001286"
     CDS             complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="IXH60_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1990224.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="IXH60_001287"
     CDS             complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="IXH60_001287"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1990225.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6307..8385)
                     /gene="topA"
                     /locus_tag="IXH60_001288"
     CDS             complement(6307..8385)
                     /gene="topA"
                     /locus_tag="IXH60_001288"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1990226.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="IXH60_001289"
     CDS             complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="IXH60_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1990227.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9420..10187)
                     /locus_tag="IXH60_001290"
     CDS             complement(9420..10187)
                     /locus_tag="IXH60_001290"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1990228.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="IXH60_001291"
     CDS             complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="IXH60_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1990229.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11300..11698
                     /locus_tag="IXH60_001292"
     CDS             11300..11698
                     /locus_tag="IXH60_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1990230.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            11935..12477
                     /locus_tag="IXH60_001293"
     CDS             11935..12477
                     /locus_tag="IXH60_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1990231.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12560..13111)
                     /gene="lepB"
                     /locus_tag="IXH60_001294"
     CDS             complement(12560..13111)
                     /gene="lepB"
                     /locus_tag="IXH60_001294"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1990232.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(13263..13490)
                     /locus_tag="IXH60_001295"
     CDS             complement(13263..13490)
                     /locus_tag="IXH60_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1990233.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(13487..14005)
                     /gene="msrA"
                     /locus_tag="IXH60_001296"
     CDS             complement(13487..14005)
                     /gene="msrA"
                     /locus_tag="IXH60_001296"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1990234.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(14025..14738)
                     /locus_tag="IXH60_001297"
     CDS             complement(14025..14738)
                     /locus_tag="IXH60_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1990235.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(14741..15637)
                     /locus_tag="IXH60_001298"
     CDS             complement(14741..15637)
                     /locus_tag="IXH60_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1990236.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(15795..16637)
                     /locus_tag="IXH60_001299"
     CDS             complement(15795..16637)
                     /locus_tag="IXH60_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1990237.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            16769..>17267
                     /locus_tag="IXH60_001300"
     CDS             16769..>17267
                     /locus_tag="IXH60_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1990238.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGM"
BASE COUNT         4584 a         3598 c         3023 g         6062 t
ORIGIN      
        1 aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac cttggtaaac
       61 ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg aatattttgt
      121 cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca agtttccact
      181 actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag gcgcttcttt
      241 tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag ttacgctatg
      301 ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc
      361 atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac ctaacgtctt
      421 ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga
      481 aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat tgaaagcagg
      541 atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt gataatgaac
      601 cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa atggatactt
      661 ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc catgttcaat
      721 gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg ttttcccttg
      781 ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct
      841 gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt ttgatttcag
      901 actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat tttgaataat
      961 aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag gctggaacaa
     1021 acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta actcgtcttt
     1081 aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac gagattcaat
     1141 gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag tgatgccgat
     1201 actatcggca atattagaag cagtcagacg accttcatct ccatccaatg cttcaaagat
     1261 agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt ggacagccgt
     1321 tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct gcataccgac
     1381 aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt gttccatacg
     1441 agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga cagtcgtcag
     1501 accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac tagtaattgg
     1561 gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt caggaaattg
     1621 tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt cattataacc
     1681 tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac cacctagtgt
     1741 gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa
     1801 aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc tccgattttt
     1861 tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt gtcatccaca
     1921 tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa taatagatct
     1981 tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg
     2041 ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa cgtaactgac
     2101 acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt tggttcggtc
     2161 agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt
     2221 ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc tgaagcaatg
     2281 aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga accttcgaca
     2341 attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg ctgtgattta
     2401 gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc taatttgatt
     2461 gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt gatcaataac
     2521 tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt caaagcacct
     2581 agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat tggtgcaggt
     2641 ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc cttttgtagt
     2701 tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc ttgcggttca
     2761 gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg attagtggat
     2821 tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt ttctgcattt
     2881 tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg gatcgcattt
     2941 tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac ttcagtaaat
     3001 tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt
     3061 tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt aatatcttgt
     3121 tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac ggcaacagat
     3181 ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat ttgtcttggt
     3241 gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga taatattatg
     3301 attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa tttctttcgc
     3361 actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg caaaattccc
     3421 tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg tacctgaaac
     3481 aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt gcatagattg
     3541 ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc cgttatattc
     3601 gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac ttccggcgaa
     3661 acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc ctttcatgac
     3721 aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc cgtctttttc
     3781 gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt cctcctaaat
     3841 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
     3901 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
     3961 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
     4021 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
     4081 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
     4141 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
     4201 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
     4261 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
     4321 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
     4381 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
     4441 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
     4501 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
     4561 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
     4621 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
     4681 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
     4741 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
     4801 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     4861 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     4921 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
     4981 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
     5041 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
     5101 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
     5161 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
     5221 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
     5281 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
     5341 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
     5401 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
     5461 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
     5521 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
     5581 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
     5641 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
     5701 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
     5761 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
     5821 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
     5881 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
     5941 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
     6001 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
     6061 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
     6121 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
     6181 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
     6241 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
     6301 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
     6361 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
     6421 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
     6481 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
     6541 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
     6601 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
     6661 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
     6721 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
     6781 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
     6841 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
     6901 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
     6961 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
     7021 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
     7081 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
     7141 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
     7201 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
     7261 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
     7321 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
     7381 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
     7441 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
     7501 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
     7561 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
     7621 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
     7681 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
     7741 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
     7801 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
     7861 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
     7921 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
     7981 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
     8041 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
     8101 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
     8161 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
     8221 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
     8281 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
     8341 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
     8401 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
     8461 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
     8521 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
     8581 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
     8641 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
     8701 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
     8761 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
     8821 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
     8881 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
     8941 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
     9001 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
     9061 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
     9121 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
     9181 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
     9241 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
     9301 ttccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
     9361 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
     9421 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
     9481 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
     9541 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
     9601 cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc ctgctgaata
     9661 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
     9721 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
     9781 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
     9841 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
     9901 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
     9961 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
    10021 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
    10081 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
    10141 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
    10201 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
    10261 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
    10321 cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
    10381 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
    10441 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
    10501 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
    10561 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
    10621 tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat
    10681 agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt
    10741 agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag
    10801 atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc
    10861 cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt
    10921 acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat
    10981 tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat
    11041 tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt ttttcttttt
    11101 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
    11161 ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta ttttggtttc
    11221 tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag acgaacaaaa
    11281 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
    11341 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
    11401 tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc tctctcatga
    11461 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
    11521 tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa
    11581 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
    11641 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
    11701 agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata cgataagtat
    11761 tctccgcctc tatttttttt attcgtacaa tctctttttt acagtttata aaagcagtct
    11821 tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca
    11881 cattggtgat tttatggtat ccttttatta ttaactgaca aaggagagaa tattttgaac
    11941 actttgattg caaaagacgt ttttagattg cccgaaatga atgcaagatt gttgtcgacc
    12001 tctgctcttt tattagccct tgatgtcatc ttatacaaga tagcgattgg tcctgcttat
    12061 tttcaaatca cattcggatt tttaagtatg gcgcttatgg gttatctata tggaccgatc
    12121 tgggctggtt tgcttgaaat gttgtcgaac actttaaatt tcacgatatt tggttcagga
    12181 acgtttcaag ctgcttttct tatcacggct ttccttggtg gatttattaa tggcttattt
    12241 ttatatcata aaaagataaa cctgccttcc gtcatcgctg cccaattatt gattatgatc
    12301 ccagtcagcc tttttatcaa ttcttggctg atgtctgttt tgtttggtgc agatttcaaa
    12361 gtaatttttc taggtagatt catcaggaat atgatccaaa tccctattca aataatggtt
    12421 gtttattcat ttatcaaaac tttagaggct agaaaagtgt ttaaacgagc tgtttgattt
    12481 tacgtactaa ggtagtaaac tgattttgga ttaaacaata aagcgtgcag tctcaacaga
    12541 ctgcacgctt tattgtttct cactggatcc attttatttc atcaagtgga taatagacaa
    12601 atcttgcttt acctagaata tcagttttat tgatcgtgcc aaacgaacga ctatcttttg
    12661 aaactcttcg attatctcca agtacaaaat aattatcttc cggaagcttg gattgtcccg
    12721 ttagctcttg tagtgtaaag tccgtcgtat aggatgcatt tgcatgatct gatttcaggt
    12781 ttttagttag ataaggttcc ttaactactt tcccattgat cttcaattga tcattttggt
    12841 agctgacatt ttctcctgga agaccgatta ctcgtttgat ataaatcgtg ccatctgcta
    12901 attgaaaaac aacaatatca aatcgtctga tctccgaaaa tttttccatc acaaccatat
    12961 caccttgctt caacacgttt tccattgaat tgccttccac tggtaccggg atgaataaaa
    13021 atccccgcaa catgaacgca aggaatacag aaacaagtaa gtatttgaat acaagccaga
    13081 agcgattaat atagcgttgt ttttttgtca atctctatca atccatccct ctttttttca
    13141 ttctttcttt attataagaa aaatatcaaa aaaatgcgac tagatagctg aaataaaaat
    13201 aattctttca attaggctat tggacaaaaa acttttttga tcataaagta caaaactatc
    13261 tcttatatgt catgattatt ttctaaatat ttttcccatg cttcgtcaaa gatatccatg
    13321 gacggcaaat agtcgacatt cagttccaga taattagaaa tttcttcgta gctagagctg
    13381 tgcttaggaa attggatgtc tttaccggct tctgtagcga aaacagataa agcatcatgt
    13441 gtgccaccgc ctcgcagtgt cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc
    13501 tccaattttc ttcgataaag gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat
    13561 gctttttata atactcttga tggtcttctt ctgctagata aaatggttgg gctggttcga
    13621 tttttgtaac gattggctgt tcgaaacgtc cactttcagc taactgtttc ttactttttt
    13681 cagcaatgat ccgctgttct tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc
    13741 gatcctcgaa ttgtccgaaa gcgtcagttg gatcagtttg ctgccagtaa agttctacta
    13801 attgttcata tgaaatcaat gttggatcat agataatctc cactgcttct gtatgtccag
    13861 tcgtttggct ttttacttgt tcataagtcg gatctggtac atgccctcca gtatatcctg
    13921 acatcactgt atggatacca ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc
    13981 ctcctgcaaa tattgctcgt tccattatga ttccccctct aatactactc atccgatgat
    14041 tttttatttt cttttgtctt gctttcagta ctgctggtgc tagttttgtt ggtatttgag
    14101 ttgtttgaag catcagaggt atcttttggc aaatatagac taaaccgaat gtcatcattt
    14161 actaagtcga ttttatctgc tttaatatac atcccgtttt gcattcggaa ttgatccaac
    14221 ctaagcatga tagtcagatc atctgtattg acttcgatcc aatctggtaa tttatagttt
    14281 ctcttgatca tattcatcac atctttgatt ggcaaactca atgttccgat agaaagactt
    14341 ctagctttta gttggacatt tccgttttcc atcacatacg gatcaaaata taaatagaaa
    14401 tcgataggaa aacctagtac ttcgaaagta ccattgagta gtgcttcatt ttccaaataa
    14461 aatttatatt caatatcact atctttttga tactcactaa ggaaaaagct gatgatttga
    14521 ttgacttttt ctttatttga attgatcgtt aagacaggat ctccttgtct ttccgtaaga
    14581 gctggtattt ctgggagttc tggttcccga ttagcaaaaa tacggctgcc taaaaatacg
    14641 gtcgaaccaa gtactacccc aagaaggata aggaaggcga atttccagta atttgttttc
    14701 tttgttttgt ttgaagatac gtcagatctt gttttcatgt ttattgatca tcctccttct
    14761 ctaaccattg gttttgtgtc tcgatcagct gatctctcac tgcatttgcc atcaattgat
    14821 aacctaaatt atttggatgg aaatgatctt cttcatagag cacattattt ttgatgccat
    14881 ttagattttc tgtcgtgcta gttgtttgat ctgttccttc ttctgtgaca cctacttcct
    14941 gattcaatcc ttgataaagc aaatcattga ttgggataaa gtagcagttc ggcatttttt
    15001 ttgtcaccgt ttctgttcct ttgttccaat tgttaacaat tgtctgcatg tcagtgatct
    15061 caggaaagtt caaataaaat ggattgtaga tgcctaaaat gtaaataggt gcggttttat
    15121 tgagggatcg aaattcttcc aatagttctg tgacattttc ctgatagttt ttcagtggct
    15181 ttttaaagga attgaccgtt aacccgaata aattattttg gataactttc atcaaatcat
    15241 taccgccaac agtcagtgtg ataaaatcag ctgttttgat attgtttcga atttcttctt
    15301 ttgtttttac tcgctttaag atctggtcac ttcgttcacc atttacacca tagttttctg
    15361 tttcaaccgc ggtcaaatta taacggtctt ttagatcatt tgcgacaagg ggaacgaatc
    15421 ctccttgctt ggtttgatct cctactcctt cagtcagcga atctccgata gctatgtagt
    15481 gaagtttttc ttttttattt ggatcttctc tcacagtagt tactggtttc attagtgggc
    15541 cagctttcgg tatagaagca gctaaacctg catagatgat gatcgcaatt aataatggga
    15601 acaaaacgag gatcaaatgt cgtttcgtta atttcatttt ttcacctctt gtattcgatt
    15661 ggttctaatg ttatcaaaaa ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat
    15721 tattttccta attttataaa gaaaaagaga ataagctgca agcctttcag gctgagggct
    15781 tatcctcttt tcgactaatc tacataatac atgattgcaa acgcgtcttt accagtatgc
    15841 gtagcgataa ccggattcgt atgcagtaca gggatatgca tgtccgggaa taattcttgc
    15901 aaaccctctt tgaattcttg cgccaattct tttgcatccg catgtgaaat accgatttgt
    15961 cgcacatttg gcaattcttt cagttcattt ttcaaattat taaaccattt atcgaacgtt
    16021 ttctttcctc gccccttagt tacagggatt aattcagaat gggcgaaatt catcaccacg
    16081 cgtatgttca aaacactaga cagcaaacca gttgttcggc tgattcttcc accttttacc
    16141 aagttatcta gtgtggatat tccgatgtat aattttgtat tgtcttgtac ttttttgact
    16201 tcttcaagga ttttctctac tgttttacct tcttttgcta attcagctgc tcgaattacc
    16261 tgaaaagata atccttggtc agtggtatca ctgtcgataa ccgttacttt cgtttttgtt
    16321 agattactgg cttgtcttgc tgcttctact gttccactca agccttttgt catatggatc
    16381 gaaatcactt cactgccgtc ttctcctagc tcatcataca tcgagatgaa ttctccaatt
    16441 ggtggttggc ttgttttggg cagatttttt gctttcgcca tcatttccat aaatctttcg
    16501 ccgtctagtt ggtcatcatc gggatatact gttccgtcga tcatgatgga tagcggcatg
    16561 acacgaatgc ctaaagcatc gcgtacattt ttttccatcg tacatgaaga atctgttacg
    16621 atttttacat ttgtcattat tttctctctc tctttcttta acaagatgcc ttattcatgt
    16681 taaactacat gtaagacaat ttctattttt agtataacag acaaataatg atttttcatt
    16741 aattacacct agtaaaggag ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga
    16801 aatattaaat gaagtgttta atgcagtcac ccatggaatt gggactggat tgagtatcgc
    16861 gggactagtg gttcttttag taaaaggtgc ccacttgcat tcctccctcc atattgtctc
    16921 ttatgctatc tatggatcga tgatgattct tttattttta acgtctactt tgtttcattc
    16981 attgattttt acaaaagcaa aaaaggtgtt ccaagtattc gatcatagtt cgatttttct
    17041 tttgattgca ggaagttata ctcctttttg tttgctgagt atccgaggat ggctaggttg
    17101 gactttattt gtattaatct ggttgtttgc gattcttggc attatatata aagcgttgac
    17161 gttgcataaa cgggagagag tatcaaaagt atctacgatc atctatattt ttatggggtg
    17221 gctctgtgtc gtggctgctg ttcctcttta tcactctctt ggaatga
//
LOCUS       DACEZN010000051        16686 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEZN010000051 DACEZN010000000
VERSION     DACEZN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.051"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..799
                     /locus_tag="IXH60_001301"
     CDS             <1..799
                     /locus_tag="IXH60_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1990239.1"
                     /translation="ETDARSIMNLYGNRPGASFSEKATTNKILSVSGLKTAGQINSAI
                     AFANEVKKEYGEAAISTTGHSLGGFLALYVAAEKQWKNVGLNGPDPYDLLSPEAKKWV
                     EENPGMLTNYRNHADFIGNFGGNGTGAEVRISMETAFLNLLASHSLTSWKFDENGKLI
                     IPENENNVKARKQQKENQATQLFSYQLQELNNIRKKLRLSGGRLTVNEQIYLDDTQAR
                     LVVEKLSAKMKSAMEEVIRINQRGIFELEKKWHGKKACGKHVLSPLR"
     gene            796..1002
                     /locus_tag="IXH60_001302"
     CDS             796..1002
                     /locus_tag="IXH60_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990240.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            1014..1337
                     /locus_tag="IXH60_001303"
     CDS             1014..1337
                     /locus_tag="IXH60_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990241.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            1330..1596
                     /locus_tag="IXH60_001304"
     CDS             1330..1596
                     /locus_tag="IXH60_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990242.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(1759..4104)
                     /locus_tag="IXH60_001305"
     CDS             complement(1759..4104)
                     /locus_tag="IXH60_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1990243.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            4383..5285
                     /locus_tag="IXH60_001306"
     CDS             4383..5285
                     /locus_tag="IXH60_001306"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1990244.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            5496..7271
                     /locus_tag="IXH60_001307"
     CDS             5496..7271
                     /locus_tag="IXH60_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1990245.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(7581..9167)
                     /locus_tag="IXH60_001308"
     CDS             complement(7581..9167)
                     /locus_tag="IXH60_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1990246.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            9418..9642
                     /locus_tag="IXH60_001309"
     CDS             9418..9642
                     /locus_tag="IXH60_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1990247.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9784..11535
                     /locus_tag="IXH60_001310"
     CDS             9784..11535
                     /locus_tag="IXH60_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1990248.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            11535..13319
                     /locus_tag="IXH60_001311"
     CDS             11535..13319
                     /locus_tag="IXH60_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990249.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEKYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            13776..14627
                     /locus_tag="IXH60_001312"
     CDS             13776..14627
                     /locus_tag="IXH60_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1990250.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            14723..16609
                     /locus_tag="IXH60_001313"
     CDS             14723..16609
                     /locus_tag="IXH60_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1990251.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
BASE COUNT         5254 a         3051 c         3477 g         4904 t
ORIGIN      
        1 ggaaacggat gcaaggagta tcatgaatct ctatgggaat agaccaggag caagtttttc
       61 tgaaaaagca acaacaaaca agattttatc cgttagtgga ttgaaaacag cggggcaaat
      121 caattcagcg attgcatttg ctaatgaagt aaaaaaggaa tatggagaag ctgctatttc
      181 tactaccggc cattcattag gtggattctt agcattatat gtagcagcag agaaacaatg
      241 gaaaaatgtc ggcttaaacg ggccggaccc gtatgatctc ttatcaccag aagcaaaaaa
      301 atgggtggaa gaaaatcccg gtatgctcac caattaccga aatcacgccg attttatcgg
      361 taattttggt ggtaatggca caggtgctga agttcggatc agtatggaaa cagcattctt
      421 gaatctgctt gcttcccaca gtttaacctc ttggaagttt gatgaaaatg gaaagctgat
      481 tattcctgaa aatgaaaata atgttaaagc tcgaaaacaa cagaaagaaa accaagcgac
      541 ccagttgttt tcctatcagt tgcaagaact gaacaacata cgtaaaaaac tacgattaag
      601 tggcggccgg ctgacagtta atgaacaaat ttacttagat gacacacaag ctaggttagt
      661 agtggaaaag ttatcagcaa aaatgaaaag cgctatggaa gaagttatcc gcatcaatca
      721 aagaggaatt tttgagctag agaaaaaatg gcatggcaag aaggcttgcg gcaagcacgt
      781 gttgtcgccc cttcgttgac tgaacaggaa atcatagaag cattagctag tgttgtcgcc
      841 acaaaagaga acttagtcga ttctgcgaaa gaagtctatc tgctgcgaat caataaggca
      901 agacgtatgg gggaggcctt tgatacattg gtgaaggaga tccaagacaa aatcaatgaa
      961 atcgtgacac gagatcgaga attagcacag caattcaatt aaaggagaag tagatgaaac
     1021 tagaagaatt ggaaagagat tatcgaagtg aacgaagaca attagaagct caagaagatg
     1081 aattgatcca ctttaagaat caaggattac gattgatgga gcaagaggag cagcgactgc
     1141 atcatgtatt aaagaactta gctttagata atgagcctct gtttcaagca agacgaagtt
     1201 attcgaatta tcaggagcat tatcaacaag ccctacaagt agaacgacga aaactctatc
     1261 agcagcaaga cgaactagaa cgttcctatc gtcaaaaaat cagacaaata actatggaga
     1321 aatcatctta tgaatgaatt tttaatgcaa ttactaaaag aattagagaa aaatctacat
     1381 cgatcaaatg tacgttcagt tggtatgggg atatctcagc aaatttgggc agaaaaacga
     1441 ttgacagatc aaaaccacca aatgactgca gaagaagctg ctcaagaatt ttctaaccga
     1501 catgtacact tgaaagaatc agtaaaaggg caatttgcag aaaaactaac cgaattattc
     1561 caaagacaag aaaagaaatt aaaacagttt cgttagtaaa gttatataga aaaattttgt
     1621 gaaaatggct tttcacattt ttacataaat ttgtcttaat atcgtagatg caagcttttg
     1681 aacaccattt attcggaaaa agggagctgt gacaagattt ttgtcgcagc tccctttaat
     1741 tctgattaat ctattagatc aatcatcttt atcgtctgat tctttggtct ctggattcgc
     1801 tctagcataa gctgaagcgg tgttccagta atctgtatag ttttcatcag taccgccaac
     1861 gccgaattta aaggcgcttt tcttcggacc attttttgcc caaagtgatt ccactgtttt
     1921 tcctggcgtt tggatagatt ttttattgac agtaactttt ccagctgttt gtcctgtgaa
     1981 cccagatact tttttcttca tcacatcaga tgataagtca aatttctcat cagtgccgaa
     2041 aatctcaggg tttgcttggt aagcttgatt gattagattg gcaagataat tagccgaacg
     2101 tatccttgct tggctgtcca ttccagtatt gtcatcgtgt cctgcccagc tactgatagt
     2161 gatagacgga gtagagacga tcagccatga atctctgtat tcgttggtag aaccagtttt
     2221 tccgacccaa tctgcttttc ctagatttcc gtttaagctt gaaatgacat ctttgaatgg
     2281 tgtagtgatt ttttcattga tcacactgcg catcatgtca ttcatgatcg aggccgttgc
     2341 ttcggaataa atacggacag aattactttc atgtttataa atcacattac ctgcattatc
     2401 tgtgatggaa tcaatgatat agccttcttc gtagacgcca ccattagcta aggcctggaa
     2461 tccatttgtc tgttgaagcg tcgttacatc tactgtccct aaaggagcag attcaacgcc
     2521 ccaattatca tttgctgggt agttcatctt cgccaagtag tgctcgtaag catattgttt
     2581 cgatccaccc gaattcaata cgtcttggta taagtgataa gctgggatat tgttggacca
     2641 ttccagtgac tcacggacgg tttggaaggt gttcgaccct tcattggtcg cattgacgat
     2701 tttctcgcca gcattttctc cttcttgcca agtcgttgca taatctgata cacgggattc
     2761 tgatccgaca agtcccatat cgattgcagg accgtaaaca agaaccggtt tgatggatga
     2821 accagcttga cgttctgtat caaaggcgtg gttattttga ttctggctgt aattccggcc
     2881 acccacgaat ccatagatac gtccagtcct attgtccatc aatacatttc cagtctccac
     2941 ttggctacca gcaccatcat ctaacatata gccgtaattt tctacgccgg tttgcaatgc
     3001 agcatagata tctttatcga tcgtcgaatg gatcgtatat cctttgtttt ggatcgtttg
     3061 ctgtgctttt tgataataag cttcactgac cgatgaatct gacatatcag cattatcttt
     3121 ttctgccaat tgttgtgcaa taatccttgt tgcttcgttc atcacggtat aatacaagaa
     3181 ttctctgtca gtctgttccg cgatttgttg tgggagaaag tcttttgtaa gatcataggc
     3241 tttcgcttct tcgtattgtt ctttcgtaat ttgtccttca cggtacatac tgaataaaac
     3301 aaaatctttt ctggcaagac cggcagaaag atcttctttt agcgcgcctg tattcgtgta
     3361 gggactatac gtgatagggc tttgaggcaa accagcgata taagcagctt gtggcagagt
     3421 cacatctttt gcatctacac caaagattcc tcttgcagct tcctggactc cagcaatatt
     3481 ttgcccctta ttattccgac caaatggcga gacattcaaa tacgtagcaa tgatctcatc
     3541 ttttgaaaag tatttttcta cttgtgcaga aagaacgatc tcgtttgctt tacgtttgaa
     3601 tgttgtttca tcggttagga tctgctgttt gactaattgc tgtgtcaaag tggatcctcc
     3661 agacgagccg atgccagtag cttctgaaac taatgctcgt aaaacagctt tagggacata
     3721 accttggtgt ttatcgaagt attcatcttc tgtactgata atcgcggttt tcaataaagg
     3781 cgaaatctta tcagatgaaa tagtcgtccg catcagatct gtctggattt ttgatatctc
     3841 tgtattatct gcataaacaa tctttgacgt ttccgtaatg tttcctagtt ctgtctgcag
     3901 ttctttttta gtcggcaatt gtgtgtcttc gacaagatag gcaaagtagc cagcacctat
     3961 tccgatacct agagctgcga acaacaaaag aagaacagta ataaaaacag tcaatgattg
     4021 aaacactcta aaaataacct taggaacaaa ccatttttct ttttttcttc tattctgttt
     4081 ttttcgagta tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat
     4141 tatagcaaat aataagacaa aaaaacagac aaggctgttt ttgacaggaa tttctttttc
     4201 gaatgagata aaacgtgagg aaatgatctt ttttatgaaa gagttcttaa gcttttcttt
     4261 agagaaagtt tcagttacta tggagaacag ggcaaagaaa aacaaaccat tttctttgta
     4321 catcatctta ccaatatata ggaattgttc gaaacaattg cctttcaagg aggaatccat
     4381 ccatgttatt aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggcgcggcag
     4441 aagaagcagc cagttacgga tcttcgacct ttatgatcta tacaggcgct cctcaaaata
     4501 ctagaagaaa agctatcgaa gagatgaata ttgatgccgg aaaagcattt atggcagaaa
     4561 acggactaac aaatatcgtc gttcatgcgc cctatatcat taatttggga aatacgatca
     4621 aaccagaaat gtttccattt gctgtacaat ttctgagaga agagattgtt cgtgcggaag
     4681 ctttaggtgc tactcaaatc acccttcacc caggcgctca tgtaggagca ggggcagaag
     4741 gtgggattgc tagaattatt gagggtttga atgaagtttt gacaaaagaa cagacggcac
     4801 agatcgcttt ggagacgatg gcagggaaag gtacggaaat cggtcgaaca tttgaggaac
     4861 tagcgcaaat catcgatgga gtaaaattga atgaaaaatt atccgttact tttgatacat
     4921 gtcatacgaa tgatgccgga tataatataa aagaagattt ctcaggtgtg atggaagagt
     4981 ttgataaggt catcggatta gatcggctta aagtactcca tatcaacgat tctaaaaatc
     5041 cacaaggttc tcataaagat cggcatgcca atatcggttt tggaacaatt ggatttgaca
     5101 cattgaacaa aatcgtccat gatccaatgt ttgacaacat ttcgaaaatc ttagaaactc
     5161 catatgtcgg taaagataaa aagaaagctt acgcaccata tggaaaagaa attgccatgt
     5221 tcaaagcgca agcattcgat cccgaagtgt ttcctgagtt attagcagaa gaagtcacac
     5281 tttaagtttt ctatctgaca gcaacaaaaa aggattgggc agtctgcctt gtcctttttt
     5341 gtttaagaaa caaaggagtg ctttttgcag tcgttttcgc catgctcctt tcgccccctc
     5401 tttttaaatt cagacaaaca gggtatactg atagaaaagt aaaaaacgaa aagattcaca
     5461 agcaagtagc aaaacgtgat tgaaaggacg aaaagatgct gaacacttta aaacccttga
     5521 tcaaccaact aaaaaagaaa catacatact ttattcttgg catagccgta ttatttacaa
     5581 ttattcggtt gtatttatat agtaaagcaa tctatggaac agatatgtcg gcaacctatg
     5641 atgatcagct gctgattcac tatgctcaaa atattttgga tggtaaatgg ctaggggagt
     5701 acacgactac gacactttca aaagggatct cctacagctt gtttttagta cttgcacata
     5761 aactgcatct ttcttatgca gttttgttcg gcatgttcag tattcttgcc agcatattgg
     5821 ttgcactagc gtttaaaccg atcgtgaaga acaagtatct tcttttaagt atctattttt
     5881 attttttgtt ttctccgatt agttttacac atgagtattc cactcgaacg tatcgtaatg
     5941 ctctcgtgat cccggctgtt gagatcatta tcgcttgttt actagctatt tattaccgta
     6001 aatatagttc ccgcagaaaa ttgctgccgt ggtttttagg gttagctgtt tcttttcctt
     6061 tcttttggtt cattcgcgaa gattccatct ggcttcttcc ttttactgta gtcgcgttag
     6121 gcatcagttt cgttcagatc ttgctaggac aagacttttc tttcaaagaa gcaaagaagt
     6181 taaaagaacg accatttctc ctttcgaaac aagaatggct gaaactttca ttgtttttac
     6241 tccctctagt cggtttctcc atcgtctatc agtggatcga atataaaaac gagacaacat
     6301 atggtattgc caccatcaat gaccgttcga gtggtgaatt tggacgtttg atgaaacagc
     6361 tgatccgaat agatgacgaa acgaacttaa ataaagaaaa cagtgagata tgggtctcgc
     6421 atgctgcatt aaataaagca ttagacgcat ctcctacatt tcatactgtt tctactcgta
     6481 ttgacgaatt gtatacgact catgcgtgga cccaaggcgg aaaagtaaaa gaattaccag
     6541 gagatatcat tttttgggca ttgcgagatg tggtaagcga atctggttat taccaaaaca
     6601 atgcaaaaga aaccaatgat ttttggaaaa aagtcaatca agaattagaa gaagcctatc
     6661 aaaagggaac tatcaaaaag aaaaaagaat tctatctgat gggatcaggg gatggaaaga
     6721 cgatgaatga tctgccaatc gtatttgctt ttctgaaatc aggatatgcc tatgcatggc
     6781 gatacaaaga tttccatcaa ggagggaatt atagtttcgg gacgcaagaa caagtcgaac
     6841 aggcccaaga attattgaat cttccactaa tggataattg gatcgatagc agtgatccga
     6901 gaacacctga tttccagttg acaaaaacag caaaagtcgc gaatgtcatg ataaaaattt
     6961 accaagttct ggccattccc gtgctgattg cttcaatagc agggatatta cttatgatac
     7021 taggaaccgt atttgcaaaa gctgatcgtg cctattatag ttcctctttg attgtagtca
     7081 ccggattaat actgacacaa ttggtttttc ttttcggtgt ctcgtggttt tgttcttatg
     7141 cgccagaaaa aaaggattat tttttaagtg tctacactgg cgcaggcgtg ccattaacac
     7201 aattcatcgt agtaacaagc atgctagttt tcagtaaatt gaatctgaaa aaagctttat
     7261 cttctaaata aaaagggaac tgtgacaaaa gtcttgtcgc agttcctttt tccgaataaa
     7321 cggtgttcaa aagcttgcat ctgcggtaat aagctcaacc aaacgtaaaa tatgaggaac
     7381 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgata
     7441 cacggtgttc cagaagtaac ttcttcgaaa aataagccga aatttcacaa aaatttgaaa
     7501 agcaattttc ttaaattcct tcttatttct tgaagctgcc cacttctgtc acaacctcct
     7561 ttgtgtaaaa gaatcagttt ctattcgatt tcttctatcg tttcttgcaa acgctgttca
     7621 tattttttgt ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga
     7681 acaatgatta cataagcgat ttgatcagcc aatgctagtt gagctgaggt ggcttgctcg
     7741 ccgaagaaac tttcgatttg acctggtaat cctcgtaagt tcaagtaagt cagactaatc
     7801 acagagaacc agccaagtag ttttacaaca aatgaatttt tgaatcggct ccccatctcc
     7861 gcttggctat tggtcatcat cagtaaagga atcatcgaga atggcaatgc aaaagccaga
     7921 aaaacttggg attcattcat caagttattc aaagcggtat gttcttcgat cgttcctttt
     7981 gtacttgtaa acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgactagt
     8041 cttcttagcc aaatcgggat acgcatatgg atgaagcctt ccatgatgac ttgaccggtc
     8101 aacgtaccag taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg
     8161 gacaagatac cagattttgc gacagcaatc aagataccat tgctcatagc tgatggatca
     8221 gataaagctt catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaataca
     8281 gcgacaccca tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta
     8341 gaatcccaag tagaaaagcg taccgcttgt gctacatctt cttcatcttg gtgatcgatc
     8401 ttccgagttt gagaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca
     8461 ataatcccta aagctccagt cagcggagtt tcaccgccga tgaccggtga gtctgagaat
     8521 gtatgactgt tagggataaa gccggcaaac aaactgtgcc aatcagggtt agacaagcca
     8581 acttgataga cgaaaacgaa taaaatgacg aaaatcaagc agacaaccaa agcttcgatt
     8641 ttacgaaacc cgacttttgt cagcaataag aggacgaaaa catctaacac agtgataaat
     8701 acggcaatca ctaatggaat atcaaaaagc aggtagaggg cgattgctgc accaatgact
     8761 tctgcgatgt ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat
     8821 gtcttacttg tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat
     8881 tttgctgcca tatattgtaa aagcatagca atcagactag acatcaaaat aactgacatc
     8941 aataaatatt ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata
     9001 taacccactg ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt
     9061 tttcccttgg gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt
     9121 tcaataagtc gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct
     9181 ttcctattta tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaatatt
     9241 cggtatgcct aatttttttt cgtggcgtgt cttgctggtt aatgaaagaa attttgttct
     9301 tctctaaaaa aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat
     9361 aaactttagt aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg
     9421 gtatcaacag gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt
     9481 ggctttttcc tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat
     9541 gaggacatgt tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt
     9601 cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt
     9661 tttgcgggct gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact
     9721 attcagcaaa aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt
     9781 aaaatgtcga tttttagaaa attaggttgg ttttttcgac aggaaaagaa acattattta
     9841 attgggattt tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaggtgatt
     9901 gggatcatca ttgatgagat tgccgataaa aatttatcaa taaagatcat ttcctggtgg
     9961 atcttattat tgatccttgc agctctctta caatatcttt ttcgttatat ctggcggacg
    10021 aatatttggg gaagtgcagc tcggttggag aaagaacttc ggcagcagct attcaaacat
    10081 tttacagaaa tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat
    10141 gcaacgaatg atttgaatgc aatccaaaat gtagcaggag ctggtatctt gacctttgca
    10201 gattcagtga tcacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc
    10261 ttaacactga ttgcgctgat tcctctgcct ttactagccg ttacttcacg agtgctgggg
    10321 tcaaaactcc acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag
    10381 acacaagaaa gtattactgg aatcaaagtc attaaaacat ttggacaaga aaaagaagat
    10441 ctcgcggatt ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt
    10501 ttagactcct tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca
    10561 attattttag gtggaaagta tgtcttggaa ggcacgatct cgatcgggca gatggtctca
    10621 tttattagct atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac
    10681 gtattggaaa gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg
    10741 catatcgtgg aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct
    10801 atttcctctt ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgtg
    10861 atccaagaag gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacgatc
    10921 atcaaattac tgatgcgtga atacgatgat tatcaaggaa aaatccaatt tggcggacgt
    10981 tcgatcaaag aatattcatt gaatgcttta ttaggatcaa tcggctatgt gcctcaagat
    11041 cacttcttgt tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca
    11101 gaagaagaag tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg
    11161 ccaaaaggtt atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa
    11221 caaaggattt cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat
    11281 gcactttctg cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata
    11341 agaaaagaga aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa
    11401 gaaatcattg ttgtcgatca agggaaaatc attgaacgag gaacccatca tgagctgctt
    11461 gcattaggcg gctggtatga acgaatgtgg gagaaacagc aattagaagc gaagatagaa
    11521 ggaggcgagg cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta
    11581 aagaacagaa gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt
    11641 tcatcgttgc tatcttattt gcgtttgctc tgtctgtgat caatgtccta ttgccaagga
    11701 tcatccaaac ttttatggat gatcatctgg ctaaacagtc ggctacgaca caagttattt
    11761 tgttttttgc tggtatgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc
    11821 aatggtattt gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat
    11881 ttgaaaagct gcatacacta ggtatgcgtt attttgatca gacaccggca ggatcgaccg
    11941 tttctcgagt gacaaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg
    12001 tattgactgg gatctttgca gtcgtttctt ccttcattgc catgtttcaa atcaatggag
    12061 aaattgcctt gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc
    12121 agaaattcag ttctcgtatt taccggaata tgcgggaaaa attaagtcag ctgaacacga
    12181 aattgaatga atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt
    12241 tagctaaaga atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca
    12301 aaacgaattc gattctgtta agtccgatca tcaattttct atatgcattg gcgattgcgt
    12361 tggcgttgac tttgtttggg atcgatgcgc tgcattctcc cgtagaagtt ggactgatct
    12421 atgcatttac gacctatgtc cagggatttt ttaatccaat gtctcaaatg atggactttt
    12481 tgagcacctt tacagatgga atggttgccg gtagtcgtat tttgaaaatc atggatacag
    12541 aaaaatatgc accgcaacaa aatcctggag cagatcaaac gattacttca ggtaagatcg
    12601 agtttcgcaa tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt
    12661 ttgtcgctaa tccgggagag accgttgcat tagtggggca caccggaagc ggaaaaagct
    12721 cgatcatcaa cgtattgatg cggttttacg aattttatga aggccagatc ttgattgatg
    12781 atcatgacat tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc
    12841 aagatgcctt cttgttttat ggagatatcg caggaaatat ccggatgatg aatccaacta
    12901 tcacagacga acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg
    12961 aattaccaaa aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagcggac
    13021 aacgacagct gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg
    13081 atgaagcgac agcaaatatc gatactgaaa cagaaggatt gatccaagaa ggattgacaa
    13141 aaatgcgaca aggacggaca acgattgcga ttgcccatcg gctgtccacg attcgggatg
    13201 cagaattgat tttagtttta gacaaaggtc atatagtaga acgcgggaat catgaaacac
    13261 ttctagcgca aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa
    13321 aagtggttgt gacagggctc cgttcagctt taggtgataa gaaaaaaatt gtaaaaaagg
    13381 ctttccagat ttttgcataa atttggctta tcaccgataa gctgatactg gaacaccgta
    13441 tattagaagt tgggacagta gcatagtagc attttgacct aagcgctaga gtgaaaataa
    13501 aacagagctt cccagattct tcatctgtga gcggtagtgt cgtagtcacc agatttaaaa
    13561 aagattcgac agctagtcag aagtgcctaa tagccactaa gaaagcgttg acaaaagcgc
    13621 ttgctttcga taatataaag aaaacgatac aacttggata gtgattgcct gatgcaagct
    13681 aaacctgatg agtctcccat ggttccatgg acggcttttt gggtttttct ttttgttttg
    13741 gatacagccc tatatatcat ggaaaggagt aaaacatgct gattgagaaa gtactgaata
    13801 acaacgtcgt aataaccaga gatgaaagaa atcaggaaat gatcgtgatg ggaaaaggtt
    13861 tggcattcaa aaagaaagtg ggtgacccga tttttgactc atgcatcgac aaaaaatttc
    13921 agttgacaaa tgacggactt tcagaacagt tccaagaatt ggcacaagcg attcctcttg
    13981 agtacttaga aatttcttgt gaaatcattg aatatacaaa agaggctcta caagtccagt
    14041 tgaatgattc agtgtatatt gcattgacgg atcatttgta tacggctatc gaacgtcaca
    14101 aaatgggaat atttgtacca aatgtattgc tgtatgatat caaacaacta tttcctaaag
    14161 agtatgccat cggggaaaaa acagtgaaga aaatctcgga taaataccac atcaagttat
    14221 cagaagatga agccggattt atcgcattgc atttagtcaa tgcacaaggc attcaaagcc
    14281 aacaagacaa tacctacctg tttactacaa caatacagga tattctaaat atcgtccgct
    14341 attactataa gattacattt gatgaagatt cagtccattt ttttcgattc gtcacacacc
    14401 tacgtttttt tctgacacgc gtcctagatg gagaaaaatc agaagtggga gaagtttctg
    14461 aggaattgta ccaagttatt caaaaaaaat ataaggatac gtttggttgc gttgagaaaa
    14521 tagcagactt tttagccgag atattcaact accagatgtc cacggacgaa aaaatgtatt
    14581 taacgatcca tactgcgcgt ttagtgcatg agaatcgttg taaataaata aaaaaggagg
    14641 ataaattaac aagaacgcat gaaataaggg tagtgacatt cagttggcta aaccttccac
    14701 taataaattg gagggaatag aaatgggaaa atatcaagaa ttagcgagca aaattgtaga
    14761 aaatgtcggc gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt
    14821 caaattaaaa aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtgttgt
    14881 gacggtaatg cgagcgggag gacagtacca agttgtcatc gggaatcatg tgccagtcgt
    14941 gtttgaagag gttataagag caggaaacct tacttttgat gaggctgttt caacagaaaa
    15001 aatgagaccg tttgatatgc ttattgatgg aatatcgggc tgtttccagc cattccttgc
    15061 tattctagct gctggtggga tgattcgtgg actgacagca ttacttgtct tcttaggcgc
    15121 atttgaccgc ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca
    15181 atttatgcct gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt
    15241 agggatgttg cttggggcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg
    15301 tgcagcagaa gcaccactga cgaccatatt ttctggcact atttttgaag cacctattta
    15361 tcaaacagta ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat
    15421 tatctttatt gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga
    15481 gatgatcgcg aatttcttcg ttccattctt caccgttctt attacaatgc ccttaggttt
    15541 tttactcgtt ggaccagcat tcacattcgc aactgatatt ttaatggctg gatttgaaac
    15601 acttttagca ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct
    15661 aatgatgttt ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa
    15721 tggctggcaa aatatcatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact
    15781 tactgcactc tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat
    15841 tgtttctggt attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa
    15901 aaaaatattt atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat
    15961 gaatttaact tcttggaatc aaggtggact aggcatcttt acaattccca actatattag
    16021 accagatggt aatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt
    16081 ggtatccttt gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag
    16141 acagaaaaca tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact
    16201 gaatgcagca aaggatgcag cttttgcaca aggaacatta ggtcgaggaa tcttgatcta
    16261 ccctgaaaaa ggagaagtac gtgccccttt tgacggaaca gtcatgaccc tgttcccgac
    16321 gaaacatgca atcggaatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt
    16381 ggatactgtt cagttagaag gaaaatattt taaaagcttg gtgcagcaag gagaaagagt
    16441 gaaacaaggc gatctgttgc tccgcttcga caagaagaaa atcgaagagg aaggatacag
    16501 tttagaaaca cctgtgatcg tcaccaatta cacggattat ttggatattt tggaaaatca
    16561 aggggagacg gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg
    16621 aaagaatgga ctaggcttaa ttattctttt tagtccactt ctttattttc aaaattgaaa
    16681 ggctct
//
LOCUS       DACEZN010000052        16657 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEZN010000052 DACEZN010000000
VERSION     DACEZN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.052"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..192)
                     /locus_tag="IXH60_001314"
     CDS             complement(<1..192)
                     /locus_tag="IXH60_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1990252.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYT"
     gene            complement(194..391)
                     /locus_tag="IXH60_001315"
     CDS             complement(194..391)
                     /locus_tag="IXH60_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1990253.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(785..2512)
                     /locus_tag="IXH60_001316"
     CDS             complement(785..2512)
                     /locus_tag="IXH60_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1990254.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2505..3698)
                     /locus_tag="IXH60_001317"
     CDS             complement(2505..3698)
                     /locus_tag="IXH60_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1990255.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3885..4529)
                     /locus_tag="IXH60_001318"
     CDS             complement(3885..4529)
                     /locus_tag="IXH60_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1990256.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4623..4757)
                     /locus_tag="IXH60_001319"
     CDS             complement(4623..4757)
                     /locus_tag="IXH60_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1990257.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4759..6693)
                     /gene="feoB"
                     /locus_tag="IXH60_001320"
     CDS             complement(4759..6693)
                     /gene="feoB"
                     /locus_tag="IXH60_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1990258.1"
                     /translation="MIARDYLVQEQPELIINIIDATNLERNLYLTTQLLDLDIPMIIG
                     LNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAKKRPSIPVP
                     CTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQKEIEQLIQ
                     LTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATNRWLALPIF
                     AFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQELILNGII
                     AGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSFIPMLIATG
                     CGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSWVAPSAYFL
                     GMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKSFVKKAGTI
                     IFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGTVAAITGLV
                     AKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAPCFAAIGAI
                     HREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILLMIGLVYVL
                     FRQPKSKNQFYTILSLEGEE"
     gene            complement(6690..6890)
                     /locus_tag="IXH60_001321"
     CDS             complement(6690..6890)
                     /locus_tag="IXH60_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HAQ1990259.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     IRTFIFRTYPASILCLLIHQKK"
     gene            complement(6887..7360)
                     /locus_tag="IXH60_001322"
     CDS             complement(6887..7360)
                     /locus_tag="IXH60_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1990260.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7761..7985
                     /gene="nrdH"
                     /locus_tag="IXH60_001323"
     CDS             7761..7985
                     /gene="nrdH"
                     /locus_tag="IXH60_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1990261.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8144..10303
                     /gene="nrdE"
                     /locus_tag="IXH60_001324"
     CDS             8144..10303
                     /gene="nrdE"
                     /locus_tag="IXH60_001324"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1990262.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNSLYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10353..11318
                     /gene="nrdF"
                     /locus_tag="IXH60_001325"
     CDS             10353..11318
                     /gene="nrdF"
                     /locus_tag="IXH60_001325"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1990263.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11407..12546)
                     /locus_tag="IXH60_001326"
     CDS             complement(11407..12546)
                     /locus_tag="IXH60_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1990264.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12855..13568)
                     /locus_tag="IXH60_001327"
     CDS             complement(12855..13568)
                     /locus_tag="IXH60_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1990265.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13822..14523)
                     /gene="nagB"
                     /locus_tag="IXH60_001328"
     CDS             complement(13822..14523)
                     /gene="nagB"
                     /locus_tag="IXH60_001328"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1990266.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14757..16289
                     /locus_tag="IXH60_001329"
     CDS             14757..16289
                     /locus_tag="IXH60_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1990267.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16480..>16657)
                     /locus_tag="IXH60_001330"
     CDS             complement(16480..>16657)
                     /locus_tag="IXH60_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1990268.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5176 a         3429 c         2877 g         5175 t
ORIGIN      
        1 agtataagca gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa
       61 ttcatatttt tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga
      121 atatttagga tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc
      181 attttttttc attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca
      241 ttcctgcaat acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat
      301 acactccatt tccataatat tttccactat gagtggtacc cccatagata agttgtgtct
      361 ctttaataga caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt
      421 agactattat tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa
      481 aataattcct gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa
      541 tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct
      601 acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac
      661 gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat
      721 ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt
      781 ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg
      841 cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg
      901 accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc
      961 gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt
     1021 tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt
     1081 gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt
     1141 tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag
     1201 ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat
     1261 ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta
     1321 ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag
     1381 ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta
     1441 atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg
     1501 aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag
     1561 atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca
     1621 gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct
     1681 ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt
     1741 tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc
     1801 cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat
     1861 tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac
     1921 gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc
     1981 ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg
     2041 gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg
     2101 tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca
     2161 agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga
     2221 cagtattcat caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg
     2281 tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca
     2341 agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct
     2401 gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga
     2461 tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc
     2521 acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat
     2581 tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca
     2641 cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga
     2701 cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc
     2761 tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg
     2821 tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta
     2881 agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg
     2941 gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt
     3001 ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga
     3061 acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga
     3121 gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg
     3181 ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc
     3241 aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca
     3301 cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag
     3361 ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac
     3421 ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc
     3481 aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct
     3541 ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa
     3601 atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca
     3661 aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta
     3721 aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg
     3781 tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa
     3841 taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga
     3901 tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga
     3961 acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta
     4021 tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa
     4081 cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc
     4141 ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc
     4201 tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt
     4261 acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca
     4321 cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta
     4381 gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc
     4441 actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc
     4501 gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct
     4561 catcttacta tgctctagca aaataagcaa aaaagggaag aatagcctct tgcaacgact
     4621 gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc
     4681 ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt
     4741 aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt
     4801 cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag
     4861 atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa
     4921 actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc
     4981 attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa
     5041 ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc
     5101 tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt
     5161 ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag
     5221 cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga
     5281 aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa
     5341 gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac
     5401 cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc
     5461 agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag
     5521 aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc
     5581 aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg
     5641 atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc
     5701 aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat
     5761 gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa
     5821 tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa
     5881 gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc
     5941 tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt
     6001 ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca
     6061 acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga
     6121 cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt
     6181 ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg
     6241 ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat
     6301 tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc
     6361 tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga
     6421 ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa
     6481 aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt
     6541 gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt
     6601 agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg
     6661 ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga
     6721 atagatgccg ggtaggtcct gaatatgaat gttcggattt ttttcaacga tccttctttt
     6781 ttttctactg ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg
     6841 aacaagctag ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct
     6901 ccctttgtat caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca
     6961 ctgtcagttc gattggatct cccagagggg ctagttttcg gataactatt tttacatttc
     7021 gcgtcaatcc catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga
     7081 cgatcccagt ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag
     7141 tctttttatt gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt
     7201 ttaccagtaa aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa
     7261 cgatcccgat attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt
     7321 gataaacgaa attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa
     7381 ataaatcatg tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat
     7441 aaatgagaat cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt
     7501 gatattatga gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat
     7561 agttttccac aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca
     7621 gctctttttt caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt
     7681 gtttgttgtt tgactttttt actaagagtt ttatactatc tattatagat aataagaatg
     7741 tgttagagga ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat
     7801 gtaagatggt caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg
     7861 atgaacagcc tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca
     7921 ttacttctga tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca
     7981 gctaacatga ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac
     8041 cagtttttgt cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg
     8101 caatcaagca tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt
     8161 acaagatgtc agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat
     8221 ccctttgaat aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa
     8281 tacgatgact tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat
     8341 agaagcagaa ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt
     8401 ggacgagcaa catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata
     8461 tgcgttgaaa accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt
     8521 caattcgctt tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat
     8581 gatccatcaa cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg
     8641 gggcgaattg gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg
     8701 ctcaatcaac tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc
     8761 aaatcttcgt gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt
     8821 tccagtcatg aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca
     8881 aggagctggc gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc
     8941 aaaaaaagaa aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt
     9001 acctgataaa ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata
     9061 tagtgtcgaa aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga
     9121 tcatctagtt gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa
     9181 tgaaatctca aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc
     9241 gaaccgcgag aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt
     9301 acaagttcaa acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga
     9361 tatctcatgt aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg
     9421 tcaatctgtg cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga
     9481 tgtcgtacca ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat
     9541 gggcttacac acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga
     9601 ctttacaaat atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat
     9661 cgctcgtgaa agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac
     9721 ttatttcgaa aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt
     9781 gtttgaaggc atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat
     9841 gaaagatgga ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata
     9901 tatcaacgat acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga
     9961 gaagaaaatc ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta
    10021 ttacacttct gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca
    10081 acaacacgtc gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg
    10141 catttatgaa tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca
    10201 ttacgctttc cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc
    10261 agaagaaatc ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg
    10321 cttattcata ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa
    10381 actggaatga aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat
    10441 tttggctaga tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc
    10501 aattggaaaa agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc
    10561 aatctgaaag tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg
    10621 ttttgaacaa tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta
    10681 gtacactgaa tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt
    10741 atctgcaaaa aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa
    10801 agaaaattgc cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc
    10861 tttattattt aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc
    10921 gagatgaatc tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt
    10981 tacctgaagc agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt
    11041 acgaaaacga agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag
    11101 tcaagacatt cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac
    11161 tctttccaga tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca
    11221 caagcaacca tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag
    11281 cgatgaaaga cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat
    11341 ttttcgaaaa ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt
    11401 ttatgcttat tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg
    11461 ttccattctt ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc
    11521 atagacaggt atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa
    11581 taccaagatg atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat
    11641 caaatttcca tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa
    11701 tggagagata aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata
    11761 cgggataata ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa
    11821 tattctcatt gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct
    11881 gatataggca gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat
    11941 cgctttcgga aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa
    12001 tagcaaaata aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat
    12061 ttttgagaca cttgcagata aactgactaa tatgatattg aacaaattac taagagataa
    12121 atttgctgat gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa
    12181 ttcatttaac catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat
    12241 tgtctgttcc attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac
    12301 gaccaaaaac gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac
    12361 aacgattgga tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc
    12421 cgaaagcatt ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc
    12481 gatgatcgtg agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga
    12541 ttgcatacat ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta
    12601 cgcgctgaga ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta
    12661 gacgagtact taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct
    12721 ttttccaaat aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa
    12781 aatataagga actattttta tttggctggg cttattactt gatgtaggac atctatttta
    12841 caacctcaga tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt
    12901 acggacaact cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag
    12961 atatttttgt acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt
    13021 acgatatttt tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata
    13081 aattggattt ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt
    13141 gcaatagcaa tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg
    13201 atccctaatc cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt
    13261 ttcgtgacga tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc
    13321 agccttgctt catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt
    13381 aatgcaccac tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact
    13441 aaaatatgcg tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct
    13501 ccgccaaata gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata
    13561 tcattcatct aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa
    13621 gtgcaatttt tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt
    13681 tatgaataaa cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat
    13741 ggggagctat ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc
    13801 tctttagcct actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac
    13861 aacattgtcg tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat
    13921 catccctttg attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact
    13981 tttcataatt gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc
    14041 gaagaatcgt ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc
    14101 gtcaaatgaa gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat
    14161 ttggatatcg attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag
    14221 atcttctgct tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg
    14281 gacatccatg aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc
    14341 taagtttaca gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa
    14401 agtaactggt gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc
    14461 tttgatgatt tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc
    14521 catagtgatt gctcctttat attttggtat agcccaatta tagcattctt tttttctttt
    14581 tacaataatt agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat
    14641 ccgtgttagg tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca
    14701 agtgattcac tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga
    14761 gtatactgac attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg
    14821 tcgatctgca attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta
    14881 aaagtacatt gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt
    14941 ggcaaaaaaa tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa
    15001 caatcgaaga atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg
    15061 cttttcttta ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga
    15121 aactccaaac ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga
    15181 tgagcagcgg gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg
    15241 gtggacaacg ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga
    15301 tcttagatga accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc
    15361 ttcaggattt tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga
    15421 tcaccaccca tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa
    15481 aacaagcatt gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc
    15541 aacaaaaaca aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat
    15601 caacgatggc aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag
    15661 cttcagcaag taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag
    15721 tggaaacaac tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga
    15781 ccatccgctt tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt
    15841 tattaaaaac actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata
    15901 acacaaaaat caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac
    15961 tacaatattt aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag
    16021 ctaaagcagg gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg
    16081 aacaaactaa ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg
    16141 atgaaccgac gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga
    16201 aatttccagg aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc
    16261 aacgagtcat tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc
    16321 tgtgacaaaa gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc
    16381 ttcggtatta gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta
    16441 ttacttgatg cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt
    16501 tctctttcct ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc
    16561 gctaaccgtt acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc
    16621 agccgcacct ccaataaagt acataaacat aaataca
//
LOCUS       DACEZN010000053        16484 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEZN010000053 DACEZN010000000
VERSION     DACEZN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.053"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(98..373)
                     /locus_tag="IXH60_001331"
     CDS             complement(98..373)
                     /locus_tag="IXH60_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1990269.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(616..1926)
                     /locus_tag="IXH60_001332"
     CDS             complement(616..1926)
                     /locus_tag="IXH60_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1990270.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2108..3337)
                     /gene="rpsA"
                     /locus_tag="IXH60_001333"
     CDS             complement(2108..3337)
                     /gene="rpsA"
                     /locus_tag="IXH60_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1990271.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3472..4152)
                     /locus_tag="IXH60_001334"
     CDS             complement(3472..4152)
                     /locus_tag="IXH60_001334"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1990272.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4201..4836)
                     /locus_tag="IXH60_001335"
     CDS             complement(4201..4836)
                     /locus_tag="IXH60_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1990273.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4902..6305)
                     /locus_tag="IXH60_001336"
     CDS             complement(4902..6305)
                     /locus_tag="IXH60_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1990274.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6292..7323)
                     /locus_tag="IXH60_001337"
     CDS             complement(6292..7323)
                     /locus_tag="IXH60_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990275.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7354..7575
                     /locus_tag="IXH60_001338"
     CDS             7354..7575
                     /locus_tag="IXH60_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1990276.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7740..8477)
                     /locus_tag="IXH60_001339"
     CDS             complement(7740..8477)
                     /locus_tag="IXH60_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1990277.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8630..9970)
                     /locus_tag="IXH60_001340"
     CDS             complement(8630..9970)
                     /locus_tag="IXH60_001340"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1990278.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10207..11730)
                     /locus_tag="IXH60_001341"
     CDS             complement(10207..11730)
                     /locus_tag="IXH60_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1990279.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12108..12719)
                     /locus_tag="IXH60_001342"
     CDS             complement(12108..12719)
                     /locus_tag="IXH60_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1990280.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12842..12967)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13049..13765)
                     /locus_tag="IXH60_001343"
     CDS             complement(13049..13765)
                     /locus_tag="IXH60_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1990281.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13771..14340)
                     /gene="scpB"
                     /locus_tag="IXH60_001344"
     CDS             complement(13771..14340)
                     /gene="scpB"
                     /locus_tag="IXH60_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1990282.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14324..15121)
                     /locus_tag="IXH60_001345"
     CDS             complement(14324..15121)
                     /locus_tag="IXH60_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1990283.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15091..15462)
                     /locus_tag="IXH60_001346"
     CDS             complement(15091..15462)
                     /locus_tag="IXH60_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1990284.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15481..16368)
                     /gene="xerD"
                     /locus_tag="IXH60_001347"
     CDS             complement(15481..16368)
                     /gene="xerD"
                     /locus_tag="IXH60_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1990285.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4626 a         3573 c         2793 g         5492 t
ORIGIN      
        1 gattcggaat ctctgtctct tatttattcc ttttctgcta ggaaataaaa agaaaagcac
       61 ttgtagccct agagccacaa gcgctttttc aactgtctta tttaacagcg tcttttaatg
      121 ctttacctgg tttgaatgca ggtactttgc tagctggaat ttcgatttct tcaccagttt
      181 gtgggttacg acctttgcga gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta
      241 attgaacttt ttcgccgtta gctaaagcat cttgaatagt tgaaaataca gcatctactg
      301 ctgcagttgc atctttttta gttaaatcag tagctgaagc aactttttcg attaattctg
      361 ctttgtttgc catggataga ttcacctcct ccacaagaga tatgatagaa atatcattta
      421 gttaaaactt tctgagtggt ccagaagttt aaaaagaaat attgattaac ttcaatattc
      481 tgttatcacg atatcatatg aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct
      541 ttttttagtc atagaaacgt aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc
      601 gatatggtaa aatgcctatt ttctcctacg agggataatt cggatggacg tcccttcaaa
      661 agtaaatgct tttctgattt gattttctaa gaaacgttca taagaaaaat gcatcaattc
      721 ctcttcattg acaaaaacga caaatgtagg tggtttgaca gcgacttgtg tagcataaaa
      781 aatcttcagg cgttttcctt tatctgttgg tgttggattg attgctacag catccataat
      841 gatatcattc aacacagccg aagagatacg aagattctgg ttcatactta cttgttcgat
      901 catttcaggc agacgttcca accgttgttt agtcactgca gaaacaaaaa cgattggagc
      961 gtaatcaaga taccggaatt catcacgaat ctcttgttcg aaatcacgca ttgtattggt
     1021 ttctttcttt acaagatccc atttattcac gacgataata attccacgac cagcttcatg
     1081 ggcgtaaccc gctactcgct tgtcttgttc ccgaatccct tcttcagcat tcagtaccat
     1141 caaaacgata tcagaacggt caatggcacg cattgcacgc atcacgctgt atttttcagt
     1201 attttcgtat acttttccgc gtttacgcat accagctgta tctatcatta agaatttctg
     1261 accactttca gaaacgaagt gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga
     1321 aacaattaca cgttcttctc ctaaaatcgc attgatcaaa gaggatttac caacatttgg
     1381 gcgaccaatc aaactaaact taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa
     1441 atacttcact gcttcatcta aaatatcacc aatccctagt ccatggcttc ctgaaacagg
     1501 ataaggatcg cctagcccta aggaataaaa ttcatagata tcgtttctca tttctggatt
     1561 atctacttta ttgactgcta aaatcactgg tttattactt ctgtataaaa ttttcgcaac
     1621 catctcatct gcgtctgtca cgccttcgcg tccgctgact acacaaatga ttacatctgc
     1681 ttcttcaatc gcgatttctg cttgatgctt gatctgatcc ataaaaggtt catcgccaag
     1741 atcaattccg cctgtgtcga tcacgctgaa ttctcgacct aaccattcac cttttgcata
     1801 aatacgatca cgagttactc caggagtatc ttcgacaatc gagatacgct caccagcgat
     1861 gcggttaaaa atcgtagact taccgacgtt cgggcggcca acgattgcaa ttgttggatt
     1921 tgccatatta tttcctcctc acaagtttcc aatctcactt agtttaacga atgcattgcc
     1981 aactttcaag catagattgt caattaaacg aagtattttt attccttatt gaaatactaa
     2041 tcgcttttca aaagaaaaac tgagacaaaa gcagctgctt tcatctcagt cacagataca
     2101 aaatcaatta tttttcagaa tcatcggttg ctttttcttc tacttcttct ttcaacgcat
     2161 cacctaaaat gtctcccata gtaaatccag tattttcttc tggtaattca taagactcaa
     2221 cattttttgg ttcttcttct gaagctggtt tttcttctaa agctttgata ctcaatgcaa
     2281 tacgatgttc ttctggatgg atctctaata ctttcacttt cacttcgtca ccttcatgaa
     2341 gcacttcatg tggtgtcgca atatgtttat gtgaaatttg agagatatgg actagccctt
     2401 ctactccagg gaacacttca acaaaggcac cgaagcttgt caaacgttta actgttccgt
     2461 ctaatactgt gccaactgga gcttttgtat cgatttctga ccaaggtcca ggctgtgtgt
     2521 ctttgattga caaggaaaca cgttcttgtt caggattgat agacaacact ttgacattta
     2581 catcatcatt gacagccaat acatcacttg gttttgctac atgactatgt gaaatctctg
     2641 agacatggac tagaccgtca attccgccta ggtcgacaaa tgcgccaaaa tcagtaagac
     2701 gtgcaacacg tccactgaga acatctccct catggatagt tgaaagaatt tcttttttct
     2761 tttcttctct ttcgttttcc actaccgctt tatgcgataa gatcaaacga ttttctgatg
     2821 gttcgatttc gatgatttta aagctcaatg ttttgccttt atagtctgaa aagtctgcaa
     2881 caaaatgatc ttccaccatt gatgcaggga caaaaccacg tacaccgacg tcaacgacta
     2941 aaccgccttt gacaacattt gtaacaggtg cttcgatgac cttgccctct tggaaatctc
     3001 tttcgatatc ttcccaaact tttttagcat ctaaacggcg ttttgaaagt aggtagctgc
     3061 cgttttcttt atctttgccg atcgtactga taacgactaa atctaataca tcaccgactt
     3121 ttactacttc gctgatatct tctaccggca aagttgataa ttcttttgcc ggaacgactc
     3181 cttcaactcc agccccttcg attccgacaa tcacttgttt gtcttcaatg actaaaactt
     3241 cgcccttaac aacatcgcca acttttactt cttgtacgct tttcattgcg tcttccattg
     3301 actggttgtt ctcttgattt tgatcaaatt ctgtcataac acttgtcctc ctaaccaaca
     3361 ttctgccgta agtacggtac tgtttttagt ttaattgatt attcaaaaaa aataaagcga
     3421 tttcgttcgt tttccttgaa aaaacaattg tttttcattt tttttcttaa tttaaaaaag
     3481 aagataacct tttttcaaaa caacagcttt tattgctgca acgacttctg gaatcgattt
     3541 gccagtcgta tcgatacgaa cagcatcctc tgcttgcttc agcggagaga cgtcccttgt
     3601 tgaatcataa tggtctcttt cctgtatctc tttcgtcagt gtttcaagat cagtcggaat
     3661 tcctttttct tggttctctt tgaaacgtct ttctgcacgc tcacttacgc ttgctactaa
     3721 aaagattttc acttctgcat tcggtaatac agctgtcccg atgtctcttc cgtccatgac
     3781 aacgccaccg cgttccccta tttttcgttg gacagcaact aattcttctc tgacttttcc
     3841 atgtgcagag acttcagaaa cagcttttgt gacatctggt tgacggattt ctaaagtcac
     3901 atcttttgat gctatgaata catgctgtcc atcttcttgt tgcgaaaaag agataggata
     3961 ctttttgatc agcgaaacta gcccttcttc atctgaaaaa gatacattgt tcgtaatagc
     4021 taaataagtt actgcgcgat acatggcacc ggtatctgta taaatataat tcaattcttt
     4081 tgctaatatc ttggcaacgg tacttttccc agatgaagca ggcccgtcga ttgcaatact
     4141 gatttgtgtc atgatataac tcctttttct cagaaaaaaa gccgcaaagc gcggctatta
     4201 ttatttaatt cttaattctt gaccagggta aagcataaag ttgtttggat ctaagccatt
     4261 caattcaaac aattgatcag tggtgatccc tgcacgctca gccacttgtt ttggaccttc
     4321 gcctgcttga acagtcgttg ttgaaccggc tgcagctgat gaagaaggtg ttgtttctgc
     4381 tgcattgctg cttgaatccc ctgcttgata agttgcctca cttgaggaag tcggtgttgt
     4441 ctctgctgca atactgcttt ctgacgttac acctgtatct tgagacacag tagattcttc
     4501 cgcgattgta ctagattcta ctgttgaagt ctcagttgac tcctcttttg ttgtacttgt
     4561 agaactttct actactgatg tcgaagtttc agaagaagtt gctgctgttg cattgttgct
     4621 tgatccattt ctgatccaga aataagcacc tgctggaata gcgatacata agaccagtaa
     4681 aatcaaaaat agcgaaagaa ataatgtatt tcctttcttt tgttgtctct gagaacttct
     4741 tgatgaaact tcatcatctg tatcgtagat tggttgttcc catggttctt gtgtttcgtt
     4801 attttctgta gattgatgtc tatcttttct gctcactgtt ttacctccta aactctatcg
     4861 tgtgtattat accataggaa aaagtgtctt gtcctaaaaa atcaatgttc catgtttttt
     4921 ttaaataaat ttagtaaaat cgtttcccaa tgaagttgtt cagtcttaga aaaaagcttc
     4981 ctctttggca gaagttctgc ttttgctcca tcaatatcac aacaattctg cggcttttcg
     5041 gagagttttt cgccaaaata agctaagatg aattttctgc ggcagttttc ttcattaata
     5101 tagcgaagca tctgctgcag gcggaattct ttttcttttt cctgacgttt gagccgttcc
     5161 acccaatttt ctggtttttc agactgctgg atcagctcca tccatttttt ttgcagctca
     5221 tcaaagggag cttgctttgt ggcatattcc aaataaagct caaaactttg tcgctgttct
     5281 ctagaaagtt gattaaagaa ataatggata cgttcatccc cactttgata aaaaagaatt
     5341 gctgcacttt cttcctgatc tctgcctgct cgaccgattt cttgtacata gttttctaga
     5401 ctgtctggca aatcataatg gaccacataa cggatatcag atttatcgat ccccatacca
     5461 aatgcattcg tagcaatgag aaactgcagc tgatttttta caaactgctg ttgaagttgt
     5521 cttctttgag ctgcatcaag accaccgtga tagtatccaa ctgtaaaacg ttccctgaat
     5581 aaatgataca atcgttcgac ttcttttttt gttgcacaat aaatgattgc cgctccttgt
     5641 gctctttcca tgaagtgttc aagttcttgt tctttttgct gcgttttccg aacaaaaagc
     5701 gagatatttt gccgattgac tgattgtctg atctcttttg gtggtcgatc aaatagtaca
     5761 tgacgtatat ctcttgctac taaatctgtt gctgtcgccg ttagtgccag tgtgaccggg
     5821 tttcctaaaa gctttcggat ttttcctaac tgttgatatt ctggacggaa atcaactccc
     5881 cattgagaca cgcaatgagc ctcatctact acatataacg caatattttg ttttttcaga
     5941 tgctctaaaa gagaaggatt agtcaacatt tcgggactca aaaacaaaaa tttatattgc
     6001 gatagatgac ggaggacgta tcttctttca gattgcgaaa gtaaactatt gaaagcagct
     6061 actcgtttct cccctcgttt ttgtaattgc gtcacttgat cttccattag agaaagcagc
     6121 ggtgacacga tgatgaccag accttccatt aaataccccg tcaattgata gcaaaggctt
     6181 tttcccgttc ctgtaggcaa aatagcgaga gtatcttctc catcaagcaa cgcctgaata
     6241 atttcttctt ggccgggacg aaatgaatca aatccaaaat atttttttaa ttcatttatt
     6301 aacattctgt atcgcttcct ttctcaatag atagatttga tacaaacgaa attctccata
     6361 atctaattgt ccatctacat tccattcgct aaagcgccac tctcgcacgg aatccttatt
     6421 tggttccaaa cgttcgattg tttctgcaga gagaatccac tcgtatggaa aatcatcaaa
     6481 aaacaaggcc cactcaataa gatgatctgt tactgttcct ctttttaaat gacggattgc
     6541 catgatctgg tcaattgtct gaccattcat aaacatctgc ttggtgatca tcatgctttg
     6601 attgaaattt tgctgtaata atgaatcgat caaagcgtgc caaagcgaat ctggccgttt
     6661 ttcgatttgt gctaacaaaa ggtggataca tctgctttgt tgcagataaa cctctgactc
     6721 atcattgttt tttgccagct gataaggaag cagccctgtt cgctcatttc cagataattg
     6781 gttagctaaa aaatctgctg cttcttctgg tatctccgaa aacagctgca ccaaactctc
     6841 atacgcagta tcaatcaaaa tttcacgtgg caattggctt tcactaagcc atttttttag
     6901 ttggaaagta taaaaaggac ttgtctctgc tggaagatag tcttgaatac ccgttgccag
     6961 attagaaatt acttgaacag caaatttaat gagccgccaa ctagtttccc ctgttctgcc
     7021 atagcggtca aaacgcaaac cagtatgttc tatttgatgt tcggacaaca tatcagcccc
     7081 tgcaggcgtt agctttgctt cattttcatt tatttcgatc caaccttgct gcgcaatttt
     7141 ttgcaaaatt tgataaaact tatcctgctt caattctgga aaactgttat ggataaacaa
     7201 aagttcgtgg gtaaatccat aaataagtac tgaagtcgtt cgttttccca caaggagttg
     7261 gtaaagagaa ctaatccgca gcttgtgtcc tgatgaaaat agcgataaaa taaaaacaat
     7321 catattattc tccttaagtt aggatggtta gaaatgtcac tactatgtaa acttgtacct
     7381 gaacgctgta ttgcatgcgg tctttgtcaa atcgaagcgc cagatatttt cgattatgat
     7441 gaagaaggaa tcgttttatt tgcccaagaa ccagaggctc atcaacagtt cgtgatgccg
     7501 gaagaagaaa catatgtaaa aaaagcgtat caaaaatgtc cagttcgagc gattttatta
     7561 gaaaataaaa tataaatata aacgaaactt taaaaacgta cactaagttc tctaaacttg
     7621 aaaataagcc gggatctgtt tctcttgttc cgtgaaatcc cgacttattt tgaaagattc
     7681 atttgctatc tatagttaat tgactagttt tttcacggac cctataggac ccatttatat
     7741 tatttcaact ttttcagcca gttttccaat gtattgacga tcacagtttg ctggtctgcg
     7801 ttagaaattt ctgcctctcc atctcctttt tgctcaccat agctgccaaa acctccatga
     7861 tttccgccat tgattgatac atactctgtt ctttccggta aatactgctt agcttcttga
     7921 taagcttcct gattcaatac tttatctttt gtcgccgtga tcgataaaac aggaacagct
     7981 gattctgtca atgttccttt tttgtcggga taacttgcca tgaagaatac cccttttaaa
     8041 tgtttgtctg aatgattctt attgacataa cgacttgcca ttacgccacc taatgaatgg
     8101 ccaccgatca cataatcgga atttggaaaa tcttctttga ccttatccgc tttgtttccg
     8161 cccatcactg ctagatcaag tggcattttg acaatagcga ctgaaaagcc gttttttgct
     8221 aaatcttgcg cccaaatact ataactttct ggctctacga gagcaccagg ataaaaaata
     8281 agtaatggag actttccatg attctttttc tctccttcaa aaaataagta atcttctttg
     8341 ttttttcctt cttttaccaa cacttgcgct tctgacgatg gttcgtacgt attttgctgt
     8401 atatagtaat aaccggctcc accgagtaat cctaaaatga tgactaagcc aattagtatt
     8461 cgtctaaata ttttcacttt ctcccacctt ttctatctga gtgacccctt tttcttaatc
     8521 aagtatgatt ataccttaaa aaaataagag agaaatgcaa actttccagc atcaaataaa
     8581 aaaagacgga atatccattt gaatatccgc cctttttgta tgtttatttt tatttagcac
     8641 ataaacgata cacagcttct gcataaattt ccattgcttt atacatacta cttaattccc
     8701 atcgttcatt cgcctggtgc ataaagtcag gagtatcagg gaacatcgca ccgaatgcca
     8761 cacactggtt catcgtacga gcaaaagtag cgccgcctga tacaaacggt tcagtcatgt
     8821 ctccagtaat atcgcgatat gtccccaata gatttttcac taactcactg tctagtggca
     8881 cgtataatga atcaaggaag tcaaattctt cataagtcaa cccatatttt tctgctgttt
     8941 tagttaattt agctactaaa tcatctttct tgaatgtaac agggattctc atatctacac
     9001 cgatttttgt ttgttctggt gtgatttcca aacttgcaaa gttcatcgtt agctcgccgg
     9061 attgttcatc ttctgttttg cccacgacag cttcaccagt tgcattttct tgaactaatt
     9121 ttcctaagaa attgattggg ccaaaatcaa atacttctga aagagcaatt gccagacgag
     9181 aaatagcatt cactccttgt gctgcatctt tcgcgtggat acttttacca agaacgacga
     9241 tgccttcacc ttgttcttcg aaatcgaatt cgtgtttttt caatgcttct tttacttcag
     9301 aaagcttttc tccagagtaa ggagctgcat caggaacaac attcaacgct cctccagctt
     9361 ttacagaaaa ctcgttcgtt cctggtcctg tcaagtaagc ttgcaataag cctttttctg
     9421 cataaattaa aggaaattca gcatctggcg caaatccttg cgtaattcct tcttcttttt
     9481 cgttatattt ttctaaacaa cgccataaat tttcttcatc tgtaccaaag ataaaacgaa
     9541 tacgtgtctt gaattccacg ccagcatcaa tcaaagcttt gacggcatac attgccgcaa
     9601 tagaaggacc tttgtcatct tgggatccgc gaccaactaa ccagccgtct tttatagttg
     9661 gatcgaatgg ttttgtttcc caattatttt cgtcccctgc tggaacaacg tccatatgac
     9721 acaagatacc gaataattca tctccagagc caatctctga ataaccgtaa tagccttctg
     9781 gatctttaaa tgttctaaat cctagctttt cactgatttc caatacttta tccaaagctt
     9841 caatcacttt tttaccgaag ggatgtcctt gtcctgaatc tgcttcatct agtacggaag
     9901 gaattcggat caattcgctc aatgattcta atgcttgttc atgatgttgt tctgtcacaa
     9961 attttttcat gatattatcc tcctaatatg ttaaaaaggg tcatgtcaaa ttcctccgac
    10021 acaacctctt cttctcaaat aatgacgttt taagcctgct ccatcagcaa gcagaatgtt
    10081 cttaacaaat gatgaaagaa aaaacactca ctgaatggag ctattgactt gtccacgtgc
    10141 ttatcgatag ccgtttatcg gtttcttcat tcttatgata gaccttgtac ctgtatttgt
    10201 ctaggtttag aacattgctg ctacacctag gatcacacag atggctacaa ataatatcgc
    10261 aattaattta ccggtaaatt tccaccacac tgaaacatcg aaacgagcaa ttgctacagc
    10321 acccattacg atagcagatg ttggtgtaat taagtttact aaaccagatg cagattgata
    10381 agcagtgatt actaaatcct taccgacgcc ggcaaattca cccataggtc ccatgattcc
    10441 cattgttgca gcagctaagc cggaagttga aggaatcaag aatgacatag ggatatagaa
    10501 aataaatgta ataatgatga atacacttga agataatgaa cttaatcctt gttcgcccca
    10561 atgtaagatg gtgtctgtga ttaatccatt gttcataacc acttggatac cacgagcaac
    10621 agctactacg attgctacac cgatcaaatc agaagcacca gctaaaaatt cagagacaaa
    10681 ttcagactct ttcatgccat aagtgattgc tactacgatg gacattacta agaataacat
    10741 agtaatttct gggaagtacc agtcacctaa tggaagcatg cttttaccta gaaccgtacc
    10801 tagtaccgga aggtttgtta accagtttgt caatgattca aaaatcgtga aattcgcatt
    10861 caaggttgtc caaggaatca aactgatgat catgatacca aaagtaataa agaacaatac
    10921 attaacatgt ttttgttttt tcgtgattac ttcttgacga ccgattgctt caatatcgaa
    10981 atgcttcata tcttctttac gttggttgta tactaatgat tttgttggat ctttttcgat
    11041 ctttgatgca taacgataca catatacaat agaaatagct aacaaaataa cgaacatcaa
    11101 gaatcgaaga ccaatacctt cacccatcga aatcccaaca gcttgtgaag ctacgcctgt
    11161 tgcaaaagga tttaccgttg aagctagaca gcctacttga gaccctacta atacgattgc
    11221 tacagctact actgtatcaa agcctacagc catcatgact gggatcagta aagcatagaa
    11281 tggaatcgtt tcttctgcca ttccgtaagt agagccgcct aatgcaaata acagcatcaa
    11341 tactggaata agcatttttt cttttccttt gaatttcttc acaactgaag caatcccggc
    11401 atctagtgcc cctgttttgt tgataatacc taaaaatcca ccaacaacca aaatgaagaa
    11461 agagattggg attgcagcag gtgtttcttt agttagctcc cccgctggta cacctagcat
    11521 cccattgatt ggcgccatga aaatatccca cagcccttga ggattttgtt gtactcgttc
    11581 atatgatcca gcgacgatgt tgcctgcatc gtctacacta tagtgcccag ctggtataaa
    11641 ccaagtaagt acagcgatta ctgcaataat aataaataag accgtatacg ctgaaggcat
    11701 ttgaaatttt ttcttttttg tttgttccat cttcttcttc ctttcttttc cgttagagac
    11761 aacaattttg gctctaatgt tattttatca aaaagagtat cagaacaaaa ataataacac
    11821 gcattttgta aaacttttgt tcaaaaagtt cgtttttccc tattccctcc tcttttttca
    11881 ggtttttaaa gctttcttct ttttcattgt agcgtttcct tttttttata aacaaacatt
    11941 ttcgtctttt aaaaggagaa gaatttcaca tttgcaaaga attgattgat ttttatgcat
    12001 aaatcaaaaa aatactcaaa acaaacaaat aaaacgttta cattattgca tgtcttgtat
    12061 ttttttccaa aaaaaaagac caagaaattt cttgatcttt ttgatattta tttgactgct
    12121 gtatgacgtt gttcgaactg atgttgtttt cgacttaacc atggaagaag ttgcgcatgc
    12181 agcactaaaa agacagcact cacgatgaat cctttgatca agttgaaagg aacgatccca
    12241 atcagtacat agtttgctaa agacatgccc agcatctgtg ttgcgttcag ccctaaaaac
    12301 attaagtaaa gaggcgtaat aacgaaataa tttgcgatac tcataaaaat agttagagcg
    12361 atggttcctg taatgacacc taaaaatttg tttcttcctt tatgctgttt ctttacacta
    12421 aagaaataat aaatcgggaa tgtaaagatc gttgttgata agaaactagc agtgtctcct
    12481 accaaattat ccggagaaaa acctgtagtc agcagatgta atactgaacg caagaacgct
    12541 gttgcaactc cggccgctgg accgaataag aacatgctga tcaataccga gatatcacta
    12601 aaatcgatct tcaaaaaact aaaagccggc atgatcggaa aagcaaaata ctgtaaaact
    12661 aatgcgatcg cagcaaacat cgctactgca accattttct gtacacgggt gttcttcatt
    12721 tgaaatcctc ctgttaggac tcctcttcag cgaacttttt gacgtttctc tccaaatgaa
    12781 aaaactctat tttccaggga aaatagagtt agagttattt gatcaagcgt cgtcaaataa
    12841 gctctatctt ctttatccag actatactgt cggtatcgga atttcaccga ttcagctatt
    12901 tttttgcaaa ataggtcgtg gactataacc accggtcggg aatttcaccc tgcccctgaa
    12961 gacgaacctt atataatttt tgtttacata ttcattatac atatctattc aaaaaacgca
    13021 acatctaaca cttataaaaa gtaagtgttc attttgagtg gttcagcaaa ctgctgactt
    13081 cttttttgtt cagttcacgg taatccccag gacgtaggcc ttgtaaagtc agatctccgt
    13141 atttttcacg ttttagtttt tgcacaggaa gaccaactgc ttgaagcata tttttcactt
    13201 gatgattacg tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc ttaggatcca
    13261 ctgataaaat ttggaattta gctggagatg tttttcgtcc ttcgatcttt attccttttg
    13321 tcaaaggtgc aagcatcgat tttgtagcga tcccttttac ctttgcaacg taaactttat
    13381 ccacttcatg ttttggatgt gtcaggcgct gagcaaaatc tccatcattt gtcagtagca
    13441 agatccccga agtatcgtaa tccagtcgac ctacaggata gatacgttct tttaccatcg
    13501 gcaaaagatc cgtaacaact tttcttcctt tgtcatctgc aacagcagaa ataactcctt
    13561 taggtttgta caataaatag taaccgtatt cttcttgata aattgggact tcatccactt
    13621 ccacagtgtc atgtttccca acttttatcc ctaattccct tacaacttgt ccgtttactt
    13681 ttacacgtcc tgcaaggatg aattcttctg cttttcgacg agaagtgatg ccagcgtggg
    13741 caattacttt ctgcaatctc tccattacaa ttattctcct ttattcattc catcaaagaa
    13801 tagatctggt gacacgtctt cttcaagtga aatttccatt tcttgtgtat caggcagttc
    13861 ttctaatgat ttcaaaccaa aataatctaa gaagtagcca gttgttccat ataatatcgg
    13921 ccgacctggt ccttctactc tacctttttc ttcaatcagt ttatgggcag ctagacgctg
    13981 taccgctcct gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg tgattggctg
    14041 tttataagcg atgattgcta aggtttcgac ggcggcttga gataatgtgt ttgatgtccc
    14101 ttgcgcatat cgcttaagta agggagccag ctctttttta gtcgttaata cataatggct
    14161 ttccgtttca aaaatatgca aagctgagtg cgagttttga gtgtaagatt cttttaattc
    14221 tttcactaaa cttgtgactt ctgcttcgct gagatttaac agtccgctta attcttcatt
    14281 actgatccct tcctcgccaa caataaaaag aagggcttca agttcactta atttcgtcat
    14341 gttcggtatc tcctactgct ggataaagca taattgattc atagttttct ttttgttgcg
    14401 caatgatctg cccttttttc atcaattcta gcaaagccat aaaagtagtg acaacttcac
    14461 ttttagaagg tgactcaaaa aaagaatcga aagtacagcc atgcgcattt ctttttatac
    14521 gctcttcgat aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt
    14581 ccactttttg agaaaatttc ttacgttcta agattttctg catggcacgg atcaaatcga
    14641 tttttttgac ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca tcgatgttca
    14701 taggttcttt tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt ctttcagctg
    14761 catatttata tttacggtat tctaaaagct gagtgaccaa ttcttctcga ggatcttctt
    14821 cccataattc ttcatcgtct gtgccgaatt cttggacagg aagcaatgtc ttgcttttga
    14881 ttgccatcaa tgtagcagcc atcactaagt attctcctgc aacagctagc tcgagtgttt
    14941 tcattgtatg gatataattc atatattgct ccgtgatcgt agcgatcggt atatcatata
    15001 tatcgatttc catttgctga atcagatgaa gcagcagatc gagaggaccc tcgaatacat
    15061 ctaatttaat attgatctcc gtcaactcat tcactctttc ttctttttct ttgctagcca
    15121 ttttccaatg taaggctctg tgatttcagt tgcacggatg gagacatccg ggtacttttc
    15181 ctgtaattca tctaacattt gaaagcccat attctcaccg cgataagacg gattcaaaga
    15241 aatgtcatgg atgatcagct gatcgttcgt tgcccagtaa ataccaataa tcccttgaat
    15301 gttggttgaa ccttctggcg taaagaaaaa aagttcataa tcgtcttcag attcgtacgt
    15361 atcaatctcc ttcaacaaag aagttttacc tgtcagtttc ttatggaaac tcaatagtcc
    15421 catagcaatt ttacggtttt ctttccggta atgtgttaac atcttctcac cttcctatga
    15481 ctaagccctt ggaaaatgct gcttataaac atccgtcatt cttttcttcg tgatatgtgt
    15541 ataaatctga gtcgtagaga tatccgcgtg acctaacaat tcctgtactg tccgcaaatc
    15601 agctccattt tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc
    15661 tttgtaaatc ccagcctctc gtactaactg cttcagattt ttccagattc cttgccgaga
    15721 aagacctttc ccatgattat tgacaaatac atgcatttca gatgcgtcct ttacaagtaa
    15781 tggacgtgct tcgtctaagt agcgttccag ccattggatc gcataatcac ctaaaggaat
    15841 gatccgctct ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc
    15901 tcccaatttc aatccaatca attcactgac gcgcatccca gtagcataca tgacttccaa
    15961 gattgcacga tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc
    16021 tacttctgtc agagtcaatg tacttggtaa tttctgtgct ttcttcggcg tatcgatatg
    16081 ttgcatcgga tcatgatcag tgatacgctc ttgccgtaaa aactgatgga atcttctgag
    16141 acttgagatc atccgagtaa cagttgctgt cgcttttttt tcttcatgca acttctccag
    16201 aaaacggatg actaaaaagc gatcgacatc ctgccatgaa gcaattttct gctcctgtag
    16261 aaaatgcaag tactgttcca aatcacgttc atagctttgt cgtgtattgg cggacaatcc
    16321 ccgttcaata ttcaagtagt ggagataatc gttgatttgc tctttcataa taatccccct
    16381 catgcctctc tatcatatca gataccgctt cattctgata gtctttttcc ataatctgag
    16441 caaagaaaaa gccgtctaga cactcagacg gactggtttc tttt
//
LOCUS       DACEZN010000054        16019 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEZN010000054 DACEZN010000000
VERSION     DACEZN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.054"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            270..539
                     /gene="rpsN"
                     /locus_tag="IXH60_001348"
     CDS             270..539
                     /gene="rpsN"
                     /locus_tag="IXH60_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1990286.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            554..733
                     /gene="rpmF"
                     /locus_tag="IXH60_001349"
     CDS             554..733
                     /gene="rpmF"
                     /locus_tag="IXH60_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1990287.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            765..1142
                     /locus_tag="IXH60_001350"
     CDS             765..1142
                     /locus_tag="IXH60_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990288.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1163..1312
                     /gene="rpmG"
                     /locus_tag="IXH60_001351"
     CDS             1163..1312
                     /gene="rpmG"
                     /locus_tag="IXH60_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1990289.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1336..2319
                     /locus_tag="IXH60_001352"
     CDS             1336..2319
                     /locus_tag="IXH60_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1990290.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2349..2471
                     /locus_tag="IXH60_001353"
     CDS             2349..2471
                     /locus_tag="IXH60_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1990291.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2489..4012
                     /locus_tag="IXH60_001354"
     CDS             2489..4012
                     /locus_tag="IXH60_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1990292.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIIGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSKHEIAQEMMAH"
     gene            4036..4275
                     /locus_tag="IXH60_001355"
     CDS             4036..4275
                     /locus_tag="IXH60_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990293.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4468..4929)
                     /locus_tag="IXH60_001356"
     CDS             complement(4468..4929)
                     /locus_tag="IXH60_001356"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1990294.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5124..5342)
                     /locus_tag="IXH60_001357"
     CDS             complement(5124..5342)
                     /locus_tag="IXH60_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1990295.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5460..7076
                     /locus_tag="IXH60_001358"
     CDS             5460..7076
                     /locus_tag="IXH60_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1990296.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7317..8657)
                     /locus_tag="IXH60_001359"
                     /pseudo
     CDS             complement(7317..8657)
                     /locus_tag="IXH60_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8933..9088)
                     /locus_tag="IXH60_001360"
     CDS             complement(8933..9088)
                     /locus_tag="IXH60_001360"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1990297.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9384..9716)
                     /locus_tag="IXH60_001361"
     CDS             complement(9384..9716)
                     /locus_tag="IXH60_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1990298.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRLAISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10230..10421)
                     /locus_tag="IXH60_001362"
     CDS             complement(10230..10421)
                     /locus_tag="IXH60_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1990299.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10515..10901)
                     /locus_tag="IXH60_001363"
     CDS             complement(10515..10901)
                     /locus_tag="IXH60_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1990300.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            11595..12614
                     /locus_tag="IXH60_001364"
                     /pseudo
     CDS             11595..12614
                     /locus_tag="IXH60_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
     gene            complement(12691..13449)
                     /locus_tag="IXH60_001365"
     CDS             complement(12691..13449)
                     /locus_tag="IXH60_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1990301.1"
                     /translation="MTTNHQKVLTNLDELGLLKMKEYYPHYLDSVRNQTISLTDALYN
                     LTQKQLDYQKEMIDKRAIKLARFPTIKSLEEFDFEFQPSINKHDILQLKSFHFLESST
                     NICFLGNSGVGKTHLATAIGIEACIQGVKTRFILFNDLIEKLTRASQRGTLVRTLKIF
                     TNVPLLIIDEIGYTPISKTQADWFYQLMARRYERASTIITTNIPFSMWAKNFDNTTAS
                     AAILDRLIHHSKVFKITGNSYRLKNYHLDKETIG"
     gene            complement(13465..14691)
                     /locus_tag="IXH60_001366"
     CDS             complement(13465..14691)
                     /locus_tag="IXH60_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003555353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1990302.1"
                     /translation="MRKDVYEGVRFFMINDIKPNFNELGRQYNCDPRTVKKYYLEGSE
                     NSVKKDKDKPKRPSKLDPFKEIIDQKLELNCSAMAIFKFLKKIGYQGGYTIVKDYCRN
                     KKHTEIKKATLRVETVPGTAAQVDWKEDMTLHDKFGKSYTFNLFLYVLHFSKLKFITL
                     TWDRKQDTLFSCLYEAFNHTGGVPKEIWFDNMKTVVDRARTQYRKVVFNTRFYEFSKD
                     AGFEAIACRSYRPQTKGSVESLAKLVERLKPYDYEFYDAVDLISLVNTLCYDLNYEEI
                     SQSTYQRPVDLWEREEKGKLHPVNEALLTPYFEDNIVRIVTNESMVNFRKSKYSVPTK
                     YIGCEVEIEIDPTEKFIEIFYQGEVVQSHELTGKRLNYKETDMFEILKSDVMKHKDDE
                     EIYEYIQRTLTQYDEL"
     gene            14799..14990
                     /locus_tag="IXH60_001367"
                     /pseudo
     CDS             14799..14990
                     /locus_tag="IXH60_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            15007..15441
                     /locus_tag="IXH60_001368"
     CDS             15007..15441
                     /locus_tag="IXH60_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990303.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT         5364 a         2458 c         2946 g         5251 t
ORIGIN      
        1 aaaagctact taaaacggta tttgtgtttg attggactaa taaaagtgaa ttcttatttt
       61 tcagagccta aactgcatat tcttttaaat gatttgatta tctctatgta ccgataaatt
      121 cgttttcaaa tagttatcta ttcatatgag ttttatttca tgttatttat tgttgacaag
      181 agcaactctt gttgataaag tatcagtgtc aaaacgtaac gattacgatt tgaggtgtct
      241 aaatcaaaat aaaaggagta taaaggtata tggcaaaaaa gtcgaaaatt gctaaagcaa
      301 agaaacaaat ggcgatgatt gaaaagtatg cagataaacg tcaggaatta aaagccgcag
      361 gagatcgcac cgctctcgct aaattgccaa gagattcaaa tccgaatcgt ctgaggcttc
      421 gtgatcagac ggatggtcgt ccaagagggt atatgcgaaa gttcggtatg tcccgtatta
      481 agtttagaga acttgcccat caagggttga ttcctggagt aaaaaaagca agttggtaaa
      541 aggaggaaat atcatggcag taccagcaag aaaaacatct aaggcgaaga aacggttaag
      601 aagaactcat caaactgtag taaaaccaga aatttcattt gacgaagcga atggtgatta
      661 tagacgtagc catcatgtat ctttaaaagg gtactataat ggaaagaaag taattaaagg
      721 agcttcaaaa tagtttaata tataactttt aggagggaat caaaatggaa attatttacc
      781 cacctttagt tgaagaagga ttaaagtatt atcttgaaac gacccagcaa tcgctagata
      841 aatgcacttt ttatcgttcg atggtagaga gaggaattat tactgaaacg ggtttaccga
      901 cccaacaggc aattgaaaat ggattagtca aagattacta tgaggaccaa ggcttatcat
      961 ttgatgattt tttaagaatc tatccaattt ttgaagagta tgacgaggag ttatttcaat
     1021 gtatcgatgg ttattgggag atacctgttg atatgaaaga aaatttagtt tcacaattgg
     1081 aatctggcga gttaactttt gaagatgcac aacaaattca agcatatctt gaagatcgat
     1141 aagtattgaa ggaggaagat ttatgcgtca aaatattatt ttggaatgtg tcgaaactgg
     1201 agaacgtctg tatctcacca gcaaaaataa acggaataac cctgaacggt tagaattaaa
     1261 aaagtattcg ccaaaattgc gcagacgggc aatttttaaa gaagtgaaat agctaagtgg
     1321 taaaaaggtg acaaaatggg cataccagtt acaatcgttt ctgggttttt aggggctgga
     1381 aaaacaacgc taatcaatca agccttgcaa gtctcaccct accccaggga agaaattatt
     1441 ataattgaaa atgagtttgg tgaagttggg attgatcaca aattattagt tcatagcgag
     1501 gaacgggtgt tacaactaaa taatggatgt atgtgttgca gtcttcgcag tgatttatta
     1561 gcaatattga tttctttact ggaaacaata gaagaacatc aacaaccaat ttcacaaatc
     1621 atcattgaaa ctacaggtat agcagatccc caaccaatca ttcaaacact tttaacagcg
     1681 cctcaactaa gaggaagagt atatatcgat agtttattga ctgttattga tagtgacagt
     1741 tttgaactta ttgaaagaga accacaagcc cttaagcaac ttgtcatggc agatcgcatt
     1801 tttgtgtcta gaaaacaaaa cttgaattta tctgcactaa aaaaattaca gagagaaatc
     1861 aaaagtatta accctttatc cgatattcga atgttcacac atagtgaaac gattcaaaaa
     1921 aaggattttt ataatttgaa aaaatttaat catcccttag tttttgaaag tgattcatca
     1981 gaaaatcact ataatggcaa atactatcat caacaccatc aagtacatga gtttaaatca
     2041 attctcttaa cttcagatca agacataaaa aaagattttt tactccaatg gattgactgg
     2101 ctgattttaa ccaaccaagg gagcctgtat cgttttaaag gattgattgc tttacaagag
     2161 caggatttta tagttgccat gcaagaggta aacgaacagg ttaattttca atacaccact
     2221 cgacaaaaag accaaaccac tattattcta atcggaaaaa tgttggatga agaaaggatc
     2281 aaacagagtt ttgataaatt actggcggaa agcaaataat gataagtagt gattgggggt
     2341 aatttctaat ggagaaaacg gatatatcta gtgcatatcg acgacttaaa agcccgaata
     2401 ttaaaactag aaaaagggca ttaaaaatta tcaaagaaca taaacgaaat aaaatgaaga
     2461 aattagcata attaaggaag gaaaaagaat gaagaaaatc agtgtaggac ttattgggat
     2521 agcagctcta ggattattgg gagcttgctc cagcactaat gatgcaaaag tatcgaatga
     2581 taaagatgga aaattagaaa ttgtaacaac attctatcca atgtatgatt tcaccaaaaa
     2641 catcatcggc gatgaagcga acgtggattt aatggttcca gcgggatcag aacctcatga
     2701 ctatgagccc tcagctaagg atatggcaaa agctcatgat gctgatgttt ttgtatatca
     2761 taacgaaaac atggaatcat gggtaccaaa agctaaagaa agttggaaga aagctgggcc
     2821 taatgtagta gaaggaacaa aagacatgat tttattgcca ggtagtgaag aagaagatca
     2881 tgatcacgga gaagaagatc accatcatga actagatcct catacgtggg tttcacccaa
     2941 aatggcaatt aaggaagttt caaatatcaa ggatcagtta gttaaattgt atccgaaaaa
     3001 agcaaaagta tttgagacaa atgctgaaaa gtatttgact aaactgaaac ggttggatgc
     3061 tgactatacc actagtctta aagaagcgaa acaaaaaagt tttgtgacgc aacacgcagc
     3121 ctttggatat ttagctttgg attatggatt gaaacaagtt cctattgctg gactaagtcc
     3181 tgaagaagag ccatcttctg gtcgattagc tgaattaaaa gagtatgtta aaaaaaataa
     3241 gatcaattat atttactttg aaaagaatgc taatgataaa attgcaaaaa ctttggccaa
     3301 cgaagctggg ataaaattgg aagtactgaa tcctcttgaa agtcttacaa aagaacaaat
     3361 ggataatggt gaagattatg tttcagtaat ggaagacaat ctaaaagcgt tagaaaaaac
     3421 aactatggtt gctggtgaag aggtagttcc agaaaaagaa gcgaaggatg aaaagacagt
     3481 tgctaatggg tactttaaag atgccgatgt gaaggatcgc gaattgagtg attatacagg
     3541 cgaatggcaa tctgtttatc cgttgttaaa agatggaatt ttggatgaag ttttcgatta
     3601 caaagcaaaa atcaataaag acatgacagc tgccgagtac aaagattatt atactactgg
     3661 ctataagaca gatattgata ctatcaacat taaagacaac accattgatt ttgtagtcaa
     3721 cggtgaacat catcaataca catacaaata tgttggatac aaaattttga attatgaaaa
     3781 aggaaaccgt ggtgttcgtt ttaattttga aactgatgat gcaggtgctg gtcgttttaa
     3841 atatattcaa tttagtgatc atggaattgc accaagtaag gcagagcatt tccatatttt
     3901 ctttggtgga gaaagtcaag aaaaactcta taatgaaatg cataattggc ctactttcta
     3961 tccagcttca ttgtctaaac atgaaattgc tcaagaaatg atggctcatt aaaatccaat
     4021 ttttggagga aatacatggt aagaaaagta ttattgtcta tggattccaa gaatattgat
     4081 gcttatcggc gttatcgatt tgcaaagcaa atgagattaa atgggagtac attagctgat
     4141 attgctgcaa ctttacatgt tagtattgac acagttcata gagtattaaa acgggatcct
     4201 gattgttgtt ttatcaaggt caatactgga agaagtagac gtttagcaaa acggcataat
     4261 agcttggcca attaattgat aataccagag tagatcattt ttcccttatt tggatttgat
     4321 ctgcttttta ttatgtcttg aattgtcaaa tcaagcgcaa cactttatca aagaagactt
     4381 cataaggtgt tctccaatct aaacactttc taggtctgtt attcagtttt tcaatcatct
     4441 gatttatcgt tgagttatct accgtagcta tctctttcga ttttggcaag tattctcgaa
     4501 tgagtctatt cgtattttaa tttgttcatt gttgccaagg gcatgaggat ctggaaaata
     4561 aaagggcata ttattcaatg cttctgtaac tgcgaaatga ttggagaatt cttttcctct
     4621 atctggagtg atcgttttga ccgcctcatt tggaatactg cttaaaagtt caatcatttt
     4681 ctcgctcaca taagcggctt tctttctggg gaccttcttt cctgctaaaa ggaagcatga
     4741 ttttctgtct gtcaaagtca ctaaacaagt accaccagtt ttgccagcta caatatcggc
     4801 tttccaatga ccaaaatcac ttctatcatc tgcggatttt ggacggccat ggatcgaatg
     4861 agtaaggcaa atctttccac gagactcgac atgatttttt gtatgtcttg tttttcctcg
     4921 atggtgcaat gaacggatca ggcctttatt gccgtgtgag aggggttctg tatcgaaaag
     4981 atcattgcaa attgcacgtt agatcgtgat aaaactaatt gaaaagtcca tatgttcatg
     5041 cttcaaacga ttcgagattt cctctggcga ccactgaaaa tcgacaaata aatgacgaac
     5101 aattgaccaa actcacggat catttagaat gtgataacgt ccgcattttt ttcttcgata
     5161 agcataattt ttttccgctt tggaaggtga atagtttttt tcttgtttct tctcagttca
     5221 tgtaaaatag taaacgggct atgctgaatg tcgttgccaa ttatactaat agaaaatcct
     5281 tcttcgtgaa gcagaaagat tctttctcgt tcgagtatag taagatgacg atactggctc
     5341 ataattattt tccttctagg agtgttctcg acaaacttat actagttgga tatcgattat
     5401 gactcatttt tatgtgttgc acttaaagtg taaattcaag gtatagaaag aggttcccta
     5461 tgaaaaaaat acctattaca atcttaactg gctatcttgg ttcaggaaaa acgatacttc
     5521 taaatcatat tctgtacggt gatcacggaa aggaaattgc tgtcattgta aatgacatag
     5581 gggaagtaaa tgttgatggg gtattagtcg acaagaaagg ttttaaaaga actggagaaa
     5641 aacttgtttc gatgccgaat ggatgcattt gttgtacatt acgcgaagat ttgattgttg
     5701 aactagatca attagtgcaa ttttctgact tgaatcaaat tgttattgaa gcttcaggaa
     5761 tcagcaaacc aattccaatt attcaagcaa ttatattagc taaaaaccct ttagacattg
     5821 atttgaccca acgattagag attggtgcca ttattatagt agtggatgta aaacgttttt
     5881 gggataattt cgaaacgggt gaaaagattc aggaaagaaa agctgaaggt aatgtagttg
     5941 aagaagaaca ggatattcgt ggcttgttaa ttgatcaatt cgagtgttgt aacatattac
     6001 ttttaaataa tagtgactta gtgtcaacca agacgattta caaaattaac ggtctaattg
     6061 ctaagttaca gtccaatgcc aaaataatta ggacaattaa aggtgaagta gacttatcga
     6121 aaattctaaa tacaaatctt tttaatatag atgagacttc ctattcagtg ggctggttca
     6181 aagagttaga gcttggctat caaaatcata ttcctgaaac agaagaatat ggtatctcaa
     6241 gttttatcta tcgaaatcgg ttactccttg acgcacaaaa atttgcccat cttacggata
     6301 aaaattttcc tggcaatatt acaagagcaa aagggtttct ttggtttgcc caacatagtg
     6361 atttttcgac gatctttgaa acatcgggta gctataaaag tattgaatca ttaaattatt
     6421 gggtagctag tttacctaaa attgagtgtc aaatgattat acaaaaaaat cccgaattga
     6481 aggaagcctg gcatccgttg tttggtgaac gcattaataa actagttttt attggtatga
     6541 agatggatca acaatgtatt aaggaacaac ttgagggttg tttaactgat tctcttaatg
     6601 cagataattt ccgaaaaatt tcaccatttc catggaaaaa gtctaatatg tttagccaga
     6661 tatcgttaat gaaattttta agaaaagctg gtgtcaagat aggcgatgtc ataattattg
     6721 gcattttgat tattacctct tttattccct tatttgtatt ctctatagaa aaggagaatg
     6781 aaacaactga agatgctatc aaatatgcca tcgttcgaat tgatggcaag gaaatggatc
     6841 gcttcgattt ggataagatc aatcataaac tcgtgaatta tcatcctgcg aagggacaat
     6901 ataatattgt tgagattaaa gatggaagaa ttcgagttaa agaagataat agtcctgatc
     6961 aaatcgcggt tagaactgga tggatctcaa aacccggtca aacgagtatt tgtttacccc
     7021 ataagttagt tattagtatt gagaaaaaag aaagcacaga ttactacatt tactaatctg
     7081 gaaatagatg aggaggtttt taataagaaa aagatattat tattaataat agcaggggct
     7141 ttattaagtg tgttaagcgc ttgtggacaa gaaaaagaag cggtgacaac aggcacaagt
     7201 atctttaacg ggacctgcaa tggtttagtt aacagtcggt tagattactt attttggctc
     7261 ttcgtcaatg agtgttggtg aagaaattca atagaataat cgagatactc acacggctag
     7321 acagctatct tgtctagccg tgttgttgtt ctggctttag tcagttgggt agtattctta
     7381 gttttcttaa aatattttcc tcgatataaa aagattcgtt ctcggtagtt ttgaaagttt
     7441 ctaaagccat aggcaattcg ttttagtact ttgatatggt tattcaaaca ttctataggg
     7501 ccgttggaat agggaacgcg caacgctcgt ttgatttctg tttggtattt cttgaaggtc
     7561 gtgaacactg gttggtagtg ctctggcaac tccttgtagt cttcttttag gagcgcgacg
     7621 aattttggaa cagcttgtct tctaatagcg gaaagaaaat cctggtagac ttcgtagcct
     7681 cgctttaagg agtcatcata agataatagc cgatcgacta tctccgcttc tgtcagatga
     7741 tctcgaaatg acggacgcca gtagcgatgc tggcccttca attcccacgc attcttttga
     7801 agtagtctcc agtatttctt taagtgcttc gaccgacgat cccttttttt aaaggtcttc
     7861 attacttgaa ttcggtgatt ggtaaaggcc cgatcgatgt gttggctaat atgaaagcga
     7921 tcggcgacga ttcgggcatt cggaaaacgc tctttcacca atgtgcgata gggtgtatag
     7981 aaatctgaaa ccactagacg tacttgttca cggacttttc tagggtagcg caagaaatag
     8041 ccgcgtaaag gattgagttg ccgattttcc acaatatcaa tcagttgata ggtcttgccg
     8101 tccatcatga caaagctcat ggctcccgag acatttttca ctgatttgaa ttcatcgaaa
     8161 cacaccactt ctggtaacag ctggttcaaa gaaatggttt taggccgaag gctgcgaagt
     8221 attcgaatga cagtggtcgg agaaatgtgc ttcatacgtg cgatcaaaga catagaaaga
     8281 ggttcgctta atagctcaag aatggcttgt ttgactctgc gagcaatgga gcagcgacga
     8341 tcactaactg aatcttctgc taaaaaagtc tttccacagt gtttacattt gaagcgctgt
     8401 ttgttaatcc gcagaatggt cttgtagtta ctgacatcat tcaataagat ggatactttt
     8461 ttccagccct atttaatgat cgtttgttca ttttgatagt ggcgatgaaa gcattcctcc
     8521 ggttgatagg tcaatgtgcc atcaaaagca aaacatgttt cgtcattgat gcgctctttg
     8581 cgaaaggcat cttcatgaaa ggtaagattt aggtctagaa tatctagtaa ctctttggta
     8641 aactctgaat aggacatctt gaaacactac tttgaatggt ttttgtcgac tttattctaa
     8701 aggattttaa ggtgtctgtt tgcgtttatt agaaaaaaga aaaagtattg gcaacagaag
     8761 gcgtcaccaa cactagatat tatagatccc ttattttctt cgcttcaaat tgtcaaggag
     8821 tcatcataaa ttttgtatgg attcttttgt ttgggtagta gtcttctaaa taattttgga
     8881 ttaacacctt catctctatt ttacttttat atttgttgga gtcgattaat tctcatttga
     8941 ttgatcgatg aaacgcttca attactgcat tgtcaacagg agtcccagtt tggctcactg
     9001 aatgtgtgat gtggtggtcg ttcaacagct cttgataaat aaaagaccga tgctggctac
     9061 ctctatcgga atgaattacc gtaggcaatt ctccttaata aatttgtaat ggacggacga
     9121 caatctcttt tttcatgtgt gaatctacca tatagtaacg tagaatacga gttgctaagt
     9181 caatataggt ggacaaatag aggcgaccgt cattacatgg aatgtaagta atatccatca
     9241 cccaaacagc atcaatttgg ttctgttcaa attatcgatt caacattttt ttaccatttc
     9301 tttgattgtt ttttctctac catgagaaga tttgcgtcga tgaaatcctc ttgtgcataa
     9361 attcatttca cgcataagaa cagctacaac acgtttattg atttcgattc cttcatcgta
     9421 aagtttttga gtgatccctg gagatccgta tcgatctttg tgttcgtaga acactcgtct
     9481 tcctctttta gtcgtttatt ttcctgttca agaatgtaga aatctgcttt atcaggtaat
     9541 ctttttcctt gtccagcaaa agcatttttc ccgtatcgac gatattctga aacccatcgg
     9601 gaaattgcta gacgagagat attgtattct tcagcaattt tcttggtagt tacttttttc
     9661 tcaaggatat cctgtgaaat ctttagttta aatttacgat catatgtttt tctcaataga
     9721 gttcattcct tccggtaacg taactgatag tcaagaaaag tgtatcatag cccaacttag
     9781 tatcagggtt tgtcaactaa actgtggaag ttgatagata agagttttta acaaccaaaa
     9841 ttctcttgac tatttttgaa atcagacaaa ttttgattgg acacatagtt gaataaatct
     9901 accgtcgtta cggaaggtaa atcgcatgct tttcaattct agaggagaag tcctcgttga
     9961 agtaccatag cccagttctg gattcgacct cctgcccact tcatacgtaa ataaagtaat
    10021 tttaggagtg agactgtgac aaaagttatt tttaagctaa aaacgaatta aaaaacgttg
    10081 ataattaaaa tcattcgttt tagattctat gaatcattat agaacgctgt aaaatcgata
    10141 ctttataatt ataaagaaga tagtttcaaa tttattgttc gaaatttgaa actatctttt
    10201 tacttatgtc acagactccc ttagttgagt tattctttaa tttcgaattt cgagatagct
    10261 agatatattg caagcataca ttcttgataa caatcttgat catattttcc agttctccag
    10321 gattgcttat gaatcaaaga gctaaatctt ttgagaattg cctcaacagc ttcttcattt
    10381 ccagcctttg ctaaaatgaa atcttctttt aaagaattca taaatctacc ttcttcccat
    10441 ggacttataa tctatttctt tgggaaggag gagaacgagc aaagaagcat acaaaattag
    10501 ttattatgca gatattaaac cagcattttt tgaatttttt tgtaaagtcg atgttttaaa
    10561 tttgtcgcac cctgtcttgt aatccccaga acctttccaa tttctttatc agttttatca
    10621 aagataaatt tttcgataag aaaaatttgt tcaggttctt tcaacaaagg aaggattttt
    10681 gctaatttta tatcttcaac aaggagagtc acatttaaaa cattaatcgg ttgtttaaat
    10741 tttatagtat ctttagtaaa caaatatggt gggagttgat caagttgttc atgtttttga
    10801 atttcttctt gttttttata ataagtatta gcggtgttca aaatgacctt ttcaaagaat
    10861 cgaatcaact gggcatttac gcgttcgtcg ttatctgaca tattgcacac ctcctccatt
    10921 aagatttaaa gggaaaagag gcaaaaaaca cgaaaagaag ctaatgcaat tatgctgcgc
    10981 tgcgaataac ttctataagc tcacatgcat tgataggact gctgatgttt attttcaaaa
    11041 atgttttttt agttgataat ggatagttat ctttatactt agaaaagaaa attcatatat
    11101 cttcttaaat atcaaacatg tccttatggc taagaaaatg tcttgagctt tttacgtata
    11161 ataaatcttt tctaaaacct ccttttggat aatattctta ttcgtgtata gattgtattt
    11221 tttatttctt cccccttcat aattaacaac tagaaaagag ttcgaataga cacgtaattg
    11281 aacgaaaatt tttattggat tcccaaattg gaatatttag aaacgaaaaa ttcctattat
    11341 ctgtatagtg cttagataat aacggatcat aataattatt gtctttgaac tagatatatg
    11401 taaatagtat tcatcatgat gaaaaatgat gagttctcgg tgataaaatg tactttttat
    11461 ggtggaatat acatgaaagc gataagtgga tattttataa tgaagtaagt tagcttattt
    11521 tgacagagga atagtatttt agataacgta taaaatacta tttaatgatg tagaaaggaa
    11581 agagagaaac aaaagtgttt tcagataaag aagtcttatt aaagtctctt catgtttgtt
    11641 taaagttacc tatcgtggtt tttaattccg aattcaatat gataaaagag tatcgctcag
    11701 atagaactac ttattttttt tatgattata aaaagttact aactgataat attaaaaaaa
    11761 ctaaagattt ccatttcttt actggtgatt ttaatgagat gtttcttcta tacatgttta
    11821 aaaccagata ttacttattt ggtccattta gagcaaataa tattgataaa gattttttta
    11881 aactaaaaat gaacaatttg aatgttgcaa tggctgatag agaacgactc tataattcat
    11941 tgcaaaattt gactttgtat tctttaggag acattcgaga tattctgatt ttagttcatt
    12001 atttttttac tggaaaaata gaagatctat tccataaacc gttaattgaa tatacagaaa
    12061 atctaagtaa aattatcgag caaatcaaaa tagataatct gttatcacaa aattacgatc
    12121 cagaaatata tctgtttctt tatgaaaata aaatactgga atatgttaaa aatggagata
    12181 ttcgaaattt agaaaacatg gtttttaatc tgagtaatgg aataattcca agtgttagtg
    12241 gagatacgat tcggtctgaa aaaaattatt ctatcattgt ttttgaaaag ttagcacaaa
    12301 cgtctataac attaggaatg gatataatag aagcgtatca aagtagagat gctttgatac
    12361 aagaaaacga attagctgta tcgttacctg aggtcttaaa agtgagggat tctgggattg
    12421 tttattatac taaggaaatc ggaaaaacta agatagagca tctttctccc ttaatttctt
    12481 ctgtcgttca attcatcggc ttaaatatct ataaaagaat tactgtaaag gaaatagcta
    12541 attatttttc tgtaagtgaa actaaattaa gagaaagctt taagaatgaa atgcatatta
    12601 cgatttataa ttattgtcaa tggcggaata aaaatgctgt tttatggcgg tttaaaaatg
    12661 ttgtttttcg gcggtaataa atgcctgtta ttagccgatc gtttctttat ctaagtggta
    12721 atttttcaat cgataagaat taccagtaat tttaaaaact ttagagtgat ggattaatcg
    12781 atctaaaatt gctgctgaag ctgtagtatt atcaaaattt ttcgcccaca tagaaaatgg
    12841 aatgtttgtt gtaataattg tagaagcgcg ttcatatcga cgtgccatca gttgataaaa
    12901 ccaatcagct tgtgtcttac taatcggcgt ataaccaatt tcatctatga tcagtaaagg
    12961 aacattggta aatattttta gtgttctaac taatgttccc ctctgactcg cacgagtcaa
    13021 tttttctata agatcgttaa aaagaataaa ccttgttttg actccttgaa tgcatgcttc
    13081 aatccctata gctgtagcca agtgagtttt tccaacccca ctattgccaa gaaaacaaat
    13141 attcgtacta ctttctagga agtgaaaact tttgagttgc agaatgtcat gcttattaat
    13201 gctaggctga aattcaaaat caaattcttc caatgattta atagttggga agcgcgcaag
    13261 tttaattgca cgcttatcta tcatttcttt ttgataatcc agttgctttt gcgtaagatt
    13321 atacaatgca tctgtcaaag aaattgtctg attcctcacg gaatcaaggt aatgaggata
    13381 atactctttc atttttaata agcctagctc atctaaattg gttaacactt tttggtgatt
    13441 tgttgtcatt attatttcag ctccttaaag ttcatcatat tgagtcaatg ttcgttgtat
    13501 atactcataa atttcttcat catctttgtg tttcatcacg tctgatttta atatttcaaa
    13561 catatcagtt tctttatagt tcaggcgctt accagttaat tcgtgagatt gtacaacctc
    13621 tccttggtag aatatttcga taaatttttc ggttggatca atttctattt caacttcaca
    13681 accaatatat tttgtaggga cagaatattt tgattttcta aaattgacca ttgattcgtt
    13741 ggtgacaata cgaacaatat tgtcttcaaa atagggagtt aacaacgctt cattaactgg
    13801 atgtagtttt cctttttctt cacgttccca tagatcaaca gggcgctgat aagtggattg
    13861 agaaatttct tcataattca aatcgtaaca gagcgtattc actaaactaa ttaagtcaac
    13921 ggcatcatag aattcataat cataaggctt cagtctctca actagcttag ctaaagattc
    13981 tacggagcct ttagtttgag gtcgataact ccggcacgca atagcttcaa aaccagcatc
    14041 tttactgaac tcatagaaac gagtgttaaa aaccacttta cgatattgtg tacgtgcccg
    14101 atcaaccaca gttttcatat tgtcgaacca tatttctttt gggacacctc cggtgtgatt
    14161 aaatgcttca tacaagcatg aaaataaggt gtcctgcttt cgatcccaag ttaatgtaat
    14221 gaatttcaat ttagaaaaat gtagaacgta cagaaagaga ttgaacgtgt aagatttacc
    14281 aaatttatcg tgtaaggtca tatcttcttt ccaatcgact tgagcagctg ttccaggaac
    14341 tgtttcaaca cgaagtgtcg cctttttaat ttctgtgtgt tttttattgc gacaataatc
    14401 cttaacaata gtatagccac cctgataacc aatttttttc aaaaacttga aaatagccat
    14461 agcggaacaa tttaattcta atttctgatc aattatttcc ttgaaaggat ctaacttgga
    14521 gggacgcttg ggcttatctt tgtctttctt tacagagttt tctgagcctt ccagataata
    14581 ctttttgaca gtacgtgggt cgcaattata ctggcgtccc aattcattga aattaggttt
    14641 aatatcgtta atcataaaaa atcgaactcc ttcgtataca tcttttctca atcaattaac
    14701 ctccattcag attgagttaa gaaaagtgta tagaaaaaaa tgtaggaaaa acgacatttt
    14761 tattccgccg ttttcctaca ttttatagcc gccatttaca ttatatctct aagcgaaaaa
    14821 tttctacggc taaaataatg ttgaaatcaa accatactat cagtgaagtt tctttaggtt
    14881 taggattttc agattcttca cacttttcga gagtattcaa aaagtacgca ggtgtatctc
    14941 ctaagcaata tcaattaggt ttagtggaca atttcaacaa cattagctga gaggttttat
    15001 tttattatga gactatatca tttacgtagt ttggaggagt tgaggaaatt ggattttaac
    15061 aaaaaatttt tttgggaaat ttctgatttg agttgtttcg tagaatctga gcgtctaagt
    15121 aatggtaata atagtgtgtt aaatgaatat gacaaagtag taattaataa agtaaaaagt
    15181 ttatgtgaag aagctagaga atctatcagt catattaatg gtagcaattt taagcaaaca
    15241 ttatttaatc tgattaagct agatgctaaa atatcaagtt atttattctt tttaatacat
    15301 gatgaattta tggattatca aaaattggat cagttaattg aaagagaaag ctgggaagat
    15361 tattacgtgg gtctaacttt ttcagaaaag ttaaacaact acaagttaat agattataag
    15421 tatttgagtg aaagcaatta attattcatg tagagtacta gacactttaa atatggacaa
    15481 ttcaattata aagaaaaaga atcaataatt taatgaggct gtgacaaatg tcattttttg
    15541 tttcgctaaa aacgagtgaa aaaatgttga taactccaat cattcgttta agattctagg
    15601 aatcattcag aaatgctgtg aaaccgatac tttataatta taaagaaggt gatttcaaat
    15661 tccgaagaag gaatttgaag tcaccttttt acttatgtca cagtctcatt cagtaatgcg
    15721 ggataaggct ccttattccg ctatttgaat actctgaagt ttaatctaag aaagagtaat
    15781 attttgaaaa catgcttcat cgcatagaaa tgaacttaat ttcagaaaga atacatacat
    15841 atctttttat atgttttcag ctgctgaatg gttaaaaagc acaaatatcc gaaaaaaaca
    15901 tgaagaaatg tacttttttt gttgtaatgt gcatgaaatt ctttaagtaa attagttaac
    15961 ataagtttaa taagattcct attctgtgaa taatacgtat cagatttatc ttttattct
//
LOCUS       DACEZN010000055        15994 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEZN010000055 DACEZN010000000
VERSION     DACEZN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.055"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            226..894
                     /locus_tag="IXH60_001369"
     CDS             226..894
                     /locus_tag="IXH60_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1990304.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="IXH60_001370"
     CDS             complement(1094..1324)
                     /locus_tag="IXH60_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990305.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="IXH60_001371"
     CDS             complement(1451..2833)
                     /locus_tag="IXH60_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1990306.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="IXH60_001372"
     CDS             complement(2989..4176)
                     /locus_tag="IXH60_001372"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1990307.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="IXH60_001373"
     CDS             complement(4183..5214)
                     /locus_tag="IXH60_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1990308.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="IXH60_001374"
     CDS             complement(5230..6684)
                     /locus_tag="IXH60_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1990309.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="IXH60_001375"
     CDS             6859..7764
                     /locus_tag="IXH60_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1990310.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="IXH60_001376"
     CDS             7855..8532
                     /locus_tag="IXH60_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1990311.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8813..9709
                     /locus_tag="IXH60_001377"
     CDS             8813..9709
                     /locus_tag="IXH60_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1990312.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="IXH60_001378"
     CDS             complement(9764..11134)
                     /locus_tag="IXH60_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1990313.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="IXH60_001379"
     CDS             11372..12118
                     /locus_tag="IXH60_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990314.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="IXH60_001380"
     CDS             12115..12954
                     /locus_tag="IXH60_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1990315.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="IXH60_001381"
     CDS             12955..13905
                     /locus_tag="IXH60_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1990316.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14045..14797
                     /locus_tag="IXH60_001382"
     CDS             14045..14797
                     /locus_tag="IXH60_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990317.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14816..15967
                     /gene="queG"
                     /locus_tag="IXH60_001383"
     CDS             14816..15967
                     /gene="queG"
                     /locus_tag="IXH60_001383"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1990318.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5191 a         2997 c         3221 g         4585 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaattat gctgtttttc
    13981 gtcgtacaga tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt
    14041 ggctatgaat tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat
    14101 cagccagcag gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc
    14161 taaattattc atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg
    14221 caaatggttg tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt
    14281 gttcctttct ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac
    14341 cgtcaaagca tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat
    14401 ccagtcggcc tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta
    14461 taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca
    14521 gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg
    14581 ggaagaggta aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa
    14641 tgcattagat aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag
    14701 aaaaaaatgg atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa
    14761 aaaagaaaaa ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac
    14821 attaaaagaa gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac
    14881 cacggcggaa ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca
    14941 tacttctggc tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga
    15001 tcagccgaaa tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt
    15061 gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga
    15121 tatactcaga gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg
    15181 gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt
    15241 cgctgtagca cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga
    15301 agagttcggt tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga
    15361 taaaccagga gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa
    15421 agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac
    15481 aaaagggatg atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga
    15541 tatctgccag ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat
    15601 ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa
    15661 agacttcaaa cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca
    15721 gagaaacgca ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc
    15781 agaatgttta acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg
    15841 tctaggaaca aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga
    15901 aacagaagaa gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt
    15961 aaaataaaaa aatagttaac attattcaaa gttt
//
LOCUS       DACEZN010000056        15418 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEZN010000056 DACEZN010000000
VERSION     DACEZN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.056"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            172..2232
                     /locus_tag="IXH60_001384"
     CDS             172..2232
                     /locus_tag="IXH60_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990319.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2315..2884
                     /locus_tag="IXH60_001385"
     CDS             2315..2884
                     /locus_tag="IXH60_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1990320.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3059..3865)
                     /locus_tag="IXH60_001386"
     CDS             complement(3059..3865)
                     /locus_tag="IXH60_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990321.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4003..5517
                     /locus_tag="IXH60_001387"
     CDS             4003..5517
                     /locus_tag="IXH60_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1990322.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5920..6903)
                     /locus_tag="IXH60_001388"
     CDS             complement(5920..6903)
                     /locus_tag="IXH60_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1990323.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6908..7555)
                     /locus_tag="IXH60_001389"
     CDS             complement(6908..7555)
                     /locus_tag="IXH60_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1990324.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7640..8272)
                     /locus_tag="IXH60_001390"
     CDS             complement(7640..8272)
                     /locus_tag="IXH60_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1990325.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8288..8839)
                     /gene="coaC"
                     /locus_tag="IXH60_001391"
     CDS             complement(8288..8839)
                     /gene="coaC"
                     /locus_tag="IXH60_001391"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1990326.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8836..9573)
                     /locus_tag="IXH60_001392"
     CDS             complement(8836..9573)
                     /locus_tag="IXH60_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1990327.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9570..10559)
                     /locus_tag="IXH60_001393"
     CDS             complement(9570..10559)
                     /locus_tag="IXH60_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1990328.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10817..11359
                     /locus_tag="IXH60_001394"
     CDS             10817..11359
                     /locus_tag="IXH60_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1990329.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11376..12461
                     /locus_tag="IXH60_001395"
     CDS             11376..12461
                     /locus_tag="IXH60_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990330.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12473..13279
                     /locus_tag="IXH60_001396"
     CDS             12473..13279
                     /locus_tag="IXH60_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990331.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13280..14107
                     /locus_tag="IXH60_001397"
     CDS             13280..14107
                     /locus_tag="IXH60_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990332.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14104..15177
                     /locus_tag="IXH60_001398"
     CDS             14104..15177
                     /locus_tag="IXH60_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1990333.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4961 a         2783 c         3137 g         4537 t
ORIGIN      
        1 acaaaattaa aaagtacata agttattatt ccgattttct tgaaacaata aaaaatcaaa
       61 aaaaatactc tcctaatagt ccatcttctt ttgattttat ttatataatg aaatcatctt
      121 atgaaaatta ctacttcatg tttagacaag agtagggagg actaagaata aatgtgttca
      181 aaaagacgtt caaaacaact aaataaacac gcttgtcgtt tagataagga acggtcgtgt
      241 tctgaaaaac actacgttaa gtttatgatg cgcacgatgc tgatcagttt atctgtaggt
      301 gtctatatgc atgtacccgt tcaggcaacg acacaaacca tcaaggtcgt acgtacctct
      361 gtttctgaga ccactgattc ccctatcact attactcata ctttcactga tgcaaatgga
      421 aaacagctac ttcctgcatt gacttcctat aataaagtca gttatcctcc aacgataaac
      481 cactactggt taattcctaa caaaacatta tggacatcgg aaaccggaag cagtgtgccg
      541 ctttctatct tttccgatat gactgatcct acaaaaggtg atcagttaaa acagatcact
      601 gatattttga atcatgcttt gacagatata aaggacggtg gaagcgcaca agtttcttat
      661 gtatatgaaa aagattattc acagtttgaa agaaagaaaa tcgatgttct ggcagggaca
      721 aaagtttcga tatctgatct ggtagaaagg ctgaagaacg cagatggaac acaaggagat
      781 atgtccaaag taatttcaaa caatgggaat atcgtggata cgactaatac aggaacatct
      841 actatttcct tgtcctattt tgatccaatt gctttaaaaa caatgactgc atctgctgtg
      901 atccatgttc gtgctgatca aacagctatc aaaggaaaag atcttcactt aagaacagga
      961 gagagttacg cccctaaaga tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga
     1021 aataaagaag aaatcaaaat gaacgggaca gtagatacta caactccggg agaatatccc
     1081 gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt
     1141 tatgtagaag catctgctgc ttcatcgact gtcatcactc attttataga tagtcaaaca
     1201 aataaagaac ttcaagcaga aacacatatg agtagtgtaa atcgtattcc tgatttgcgc
     1261 ctacctgctg aattttctga tcgagtgcca aatatagatt tatcgactta tgaacaaaat
     1321 gggaaagtca cttctatttc tgattggatg caagcaaacg gattagacag aaaaagtcac
     1381 tggtcggaga tccttgaagt acttcactca gaactgatga ataataggaa gcaatcagga
     1441 ggggaaatta cctttactta tgtttatata ccagacaatc gttcattaga aggtcatgat
     1501 cttattgttc atccaaacga aaaaataaac aaagaagagt tgatccaatc agcaacaaat
     1561 tccttaggta aacccgtacc gttaaaggat gtatcggtta caatcaatga agtaccattg
     1621 gaggatggtt atttgaccaa acaaacaggt gattttacga ttatgtatag ttatcatgac
     1681 ccgtattcac tccaagaact ccatgctcag tcactattac atgttttacc attaaacaaa
     1741 cagatagatc aaaaagaaca aaaaagacaa gaagaaagct tgtcagaaaa aaaacaacaa
     1801 atggctcatg cagtttctcc aacagggaaa gtaaaacaaa caagaagcct agagttagca
     1861 agaatatcta gaaaaatctc agaatctaat aaaacgataa ggcaagaagc tcatttggcc
     1921 ccacaagttt ctggattgga aaatgaaatt ttagagccta aaataaaaca acaatcagaa
     1981 aaagaatttg ttgctaaaca gtcggaagat cagaaggaat cagatgacca gaaggaaacg
     2041 aagaaaaaac gcttgtcaac tattgattcc accgatgagc atctgcagcc gcaagtacca
     2101 cttcctagtc cgggaacacc cgcacctggt ggaggtattc cttcttctgc tttaggagat
     2161 ttcatgcgag gtatggcagg aacatgggta tacgctaatc gaaatgaatt tgacgcagat
     2221 gactggttct gatttatatc tctatctgtt atagtcaaat aagtagctga gtcatatgtt
     2281 atttggcata atggagacga ggagggatag aaaaatgtta gataaaacgt taccttacgc
     2341 agaaatctgg atgacaagac cacttcaaca aaatgttcca atcacgcctt tagcggaagg
     2401 ttttgaattt cgattttatc aaaaagaaga tgaatctgct tgggcggaga ttgagacttc
     2461 cgttggggaa tttcaaacga cagatgaagc acttgagtat tttcaacaaa actttgctcc
     2521 ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt
     2581 agcaacctgt accgcttggc gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg
     2641 gttagcagtg aaaccagatt accaaggaaa agggctcgca aaagcattga ctgccagagt
     2701 cttgcaagaa tttcctaccc tccatacgga cggaccaatc tatcttcaca cacagacatg
     2761 gagccatcag gcgattgctc tttataaaaa aatgggtttt acttttattg ctgaaaatct
     2821 agacggcaca cctaatccag attatgaaaa agttatgcag gttttaagta aattagataa
     2881 ataacgaaat ggtgaaataa gtcctcagta ttgggataaa aaacgaaata gtcattcctc
     2941 atatcttact tttttgagtt aatactatcg gataactaat ggcatgccat acattcaaaa
     3001 aagaaggggc agtgacaaaa gtattgtcac tgccccttct cttgtttttt agcatgtttt
     3061 atcgtttgat ttttttagga ataaaattct tgatgaacgt aaatttttgg taatcgtttc
     3121 gactatagag gtaaccaaat acactgaaaa caactgctcc aataaaatta acaaataaat
     3181 ccttcatagt atccatgatt cccacgtcta agtaaccatt ttggatgatt gttttatctg
     3241 ttttaccatc tgcattttga ctttcaatga ttgtccgagt gatatctgga acatgataga
     3301 cagtgtttcc gtctttactt aactccacac tattgatttg ctggatgatc gtatcttttt
     3361 gcatatcgaa ttgcagtaat ttatctgctg aaaattccac aaactcccac ataacaccaa
     3421 ctgtcatcga aaaacagaag gaaacaactg ctagtaataa aggagtcaag ttcatggttt
     3481 ttgtattttt attgagcaaa taaacaaggg aaaagccgac acccccagct aaaaatccgc
     3541 tgaatgcatg tagaaaagta tcccacattg aaaaatagcc ataaaaatca cttaactctc
     3601 ctaaaactgt tgaactaaaa ataaaagata acaaaatgat ttctagcata tttggaatct
     3661 gtatctttaa tatcttttcc agaaaataag ggatttcaaa taaaattaat gtcagtaata
     3721 aaagcattgt atttccccag tgttggttcc agacctgatc cactagcata ataacgacga
     3781 tgatacgtaa taaaatatag attccgtttg tgattctacc tgcagttttt gtactgttcg
     3841 taaaaaaggt tttcaaatga ttcataaaat aactccttca tgtatatcct taattatact
     3901 aagaagcgtg tgtttaccaa agctatatga cttaaagaaa aacagagttg atattgtgaa
     3961 aaaaatcact taatatagta taaggagaaa agggggatac aaatgaaaaa attagttgct
     4021 ggtttattaa cagccgttgc acttcttgct atgagcgcat gcggcgcaga tcagaaaaat
     4081 acagcgcaga caagttctag tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa
     4141 caagtatata cagatccaaa agaaatgaaa gaatcttatg atgtcgtgat tattggagct
     4201 ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt
     4261 ttggaaaaga tgcctgttgc cggaggaaac acatctaaat catcaagcgg tatgaatgct
     4321 tcagaaacaa aatttcaaaa agaagaagga atcaaagact cgaatgatgc cttctatgaa
     4381 gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa tgcttcgata ctttgtcgat
     4441 cactctgctg atgcggtcga ttggctagat caaaacggta tcaaactgga caacttaacg
     4501 atcactggag ggatgagtga aaagaggaca catcgtccgt ctgatggctc ggctattggt
     4561 ggatacttag tggaggggtt attgaataac gtccatgagc gggaaatccc tatttttgtc
     4621 aacgcagatg tgacagacat taagaaaaat ggggaaacgg tcaatgaagt gactgtacat
     4681 gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt
     4741 tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg atttagctga ttatattacg
     4801 acaaatcaag aaggatcaac tggagatggt atcaccatga ttgaaaaatt aggtggacaa
     4861 accgttgata tggataaaat acaaattcat ccaactgtgc agcaggatga gggggtcttg
     4921 attggtgagg ttgttcgcgg agaaggagca atcttggtcg atcaagaggg gaaaagattt
     4981 gtcaatgaaa tggatacaag ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa
     5041 tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat
     5101 gaccaaaaag gatatgtcac aactggaaag acaatggaag aattagcaga aaaaatcgat
     5161 ttaccaaaag aaacactagc aaaaacagtt gatggatgga atacggatgt tgcggagaaa
     5221 aaagatgctc aattcaaccg tgccacagcc atggaacatg acttatcgtc tccgaattat
     5281 tatgcgatca aaatcgcacc aggaatccat tacacgatgg gcggtgtgaa aatcaatacg
     5341 aacactgaag tattagataa agaaaaccag ccaattaggg ggttatatgc tgctggtgaa
     5401 gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa attcagttgc ggagatcatt
     5461 atttttgggc gacaagcggg catacaagca gctcgatttg cttcggaaaa tgaatgaaat
     5521 agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga gctaataccg agggtcagct
     5581 gattgaacac tgtttagtga agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     5641 cagccaagta aaaacagctt cttatgtttt taacttggtt ggacttatta ccgcagatgc
     5701 aagcttttga acaccgttta ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt
     5761 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag aaattccttc ggaaataagc
     5821 tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta
     5881 aacgcttctg tcccagcctc ttttttgatc agcatgattt tattcagtta tcttcaaaat
     5941 gatttttccg atgtttttgt ttgcttccat atattcatgc gcttgtttga ctgcgctgaa
     6001 ttggaatgta cggtcaatga ttggccgaat cttgccattt tctagaaaag gcccagcttt
     6061 ttgcataaaa gtttgtgcga gctctgcttt atactgatca cttctaggtg tcaataatgt
     6121 accaagcagt tggacacgtt tcccaatcaa tacaccaagg tctatctcag gcacgatggt
     6181 cccacctagc ataccaatca agacccaacg tccatcaacg gctatagaag ctaaattctt
     6241 ttcccagtaa gaagcgccga tgaaatccaa aataacatcg actccccgac cttcagtgat
     6301 ttttcgtacc tcttcttgaa agtcttgttt tttatagttg atcaattcat ctgcaccaag
     6361 ttctttacaa aaggcgagtt tttcttctga tcctgcagta acgatgattt tcgcgtgaga
     6421 aagctgtcta gctaattgga tcgctgctgt tcccacacca cttgctcctg catggatcaa
     6481 gacagtttcg ccttcttgta attttccaag ccagtaaagt gtttgataag cagtcaaaaa
     6541 aacttctgag attccagcag cagaaacata gtctagcgta tcagggagaa ggattgcacg
     6601 atcggcaggc atcacagcat attcggcata accacctaga ttgactaatc cgtatacttt
     6661 tgtcccagga gtaaaagtgg agatatcact tttattctca actacctctc cagctacttc
     6721 gactcccaaa atagggtagg gaggtttcaa agagactgat tctcttttca taatatctgt
     6781 tcgattgaca gctgctgtat ggactttgat cagtaattcg ccttgttttg gtttaggaac
     6841 aggcacttca atttgttcta atacatctac agaaccagga tgcttgattg aaatggcacg
     6901 catggatcta ttcctccttt gcccattctt ggaatgctgt attcaactct ttgctacctt
     6961 cagaaagtac tagagtcgta cgagaaatat ccgtttcttc cactagagcg gcaagtacag
     7021 cagcaactgt agagcgagtg atcgtttttg atgccttctc attaggctta gttaattgaa
     7081 tggaagtaac ttcttcatcg ttagtcaatg tgactggttg aagaatgaca aaatctaagt
     7141 tagttcgttt cagccattca tctgcataat gtttagtaat ataataatct gtcatggact
     7201 caggccattt tgctggttca tctgcataaa cagcactgac catcagatag cgagagacat
     7261 gcgcattttc agctgcgatc actgttttga ttgcgccatc caagtctact tgcagaagat
     7321 tctttccttg agagccggca gcaaaaatca atacatcgat gcctttgaat gcttcggtca
     7381 ttttttccac ggaccaagtt aaatcaaaag agacatacga aacattctct ttttttacta
     7441 atgtttggga agcaacatct ctcacccctg caaaaatttc atgggaactt ggagctaaat
     7501 cttttatcag gtgtcggcca atttgtccgt ttgcacctac aacaaaaatt ttcatttgga
     7561 tcatcctttc gacgtcaaat ctttccattc aatagtaaca ccaaaagaga aaaaaatcag
     7621 agatcggcta cgatgactat cagttagtaa gagaagggtg cttgaagtat cttaacaaga
     7681 cattgcatac tgctgcagtc gcaagacctg cgacgattcc ttcaggtata ccacttgtaa
     7741 aaataaccgc tagaatagct gaataaacag catttaaatt tgttccgatg actttggcat
     7801 aatcttgctg gaataaaaag tagatcatat tcatcactaa aatcgtattg gtcaatgaac
     7861 cggaaagtcc agctaagaat aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca
     7921 gacctttata aacgaaataa ggaaccaccc caatcaagat gcgaggaata aacgcgatca
     7981 gcaaggcttt ccagcttcca tgttctgttc cgatgactgg gataaacgga gaaaagacaa
     8041 aagacaaagg tgtttggata actgtgctac ggatcatgct gatcagtcca aatgtaccgc
     8101 caagaaaagc acctagtctt ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca
     8161 tggttgttgc attgattgga ccgataggga taaaacctaa aggcgtaaca gctaataaaa
     8221 tcaaaatagc taaaaacatc gcagttaatg taaagttttt tgtatttttc ataagtactc
     8281 cttttttcta agcatttctt tcattaagag tcagcatgac ctgttcgatg atcgtctgaa
     8341 tatcagctaa ggcacctttt ccaaaatctc cacaagctaa aagagattct cttggttcaa
     8401 tttcctgata gccgacagat ctcaatgttt ccagattacg ctgagtgatc gggttttgat
     8461 acatattcgt gttcatggca ggggcaatca attttggtgt tcccactgga agcgccattg
     8521 cgactgttga aagaagatcg tctgcaatgc cgtttgctag cttgccgata atgtttgctg
     8581 tagcaggcgc gatgagaaac aagtcagctt gttttgctag ttcgatatga ttgattacct
     8641 gcggatttga ttcttgcatg acgtctgtat ggactgcgcg cttcgacaga gattgcaagg
     8701 ttaatggcgt gataaaatgt gtgctgcttt tagacataat cacatctacc tgggcaccta
     8761 atttgacgaa ttgacttgta agatcagcgc ttttgtaagc agaaatactt ccagtaacgc
     8821 ctagtaagat ctttttcatt ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc
     8881 aatctcagat ttactttgag cttcttccac tgttccatct ttagataaga gatagccgtg
     8941 gtgtgtctcg ccatggattt ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt
     9001 tatcagacta ttttttgcga ctgccagaag agattccttt ggtacatcta ctaatagttt
     9061 gaaaccaatg atttttgctt ttggctgcca ttcacgtaga taagaaatga tttttggtgt
     9121 tttcttcaaa acgagcacaa gatgctcggt gttagaagag atctttgaaa cagtggtatc
     9181 tgtctgttca ttttccgcaa accaattgtc cagttcttct tgcgttgaag aagtgggtag
     9241 gttttttgcc agctgttctt ctgaaaaact aaaagcagga gtaaaatcac tgactgccat
     9301 actgtggata atcgcatcgt agtgttcttt tttcgttagt tcttctaatt gtaaaaataa
     9361 gtccagtgca gattcgatcg tataaatcgt tatattggag tgtcttttcg gtttcagtgc
     9421 ttgtttcgta gtcacgtaat cgattgtggt atccggattg gcagctaaat aatcagctaa
     9481 tgcttgccct aatcgtcctg tcgaatgatt tgtaatagca cgtacttgat cgattttttc
     9541 agtcgttcct ccagctgtga ttagaatctt catttatggt cacctacatt tactagtgtt
     9601 cttccttggt gtgttccagc aagtagttta gatgctgttt caggcaattg atccagagta
     9661 atctcttgga cgaccagttc atctgcaatc tgcaaatccg ccatctgttg ccataaagaa
     9721 agacgttgat ctattggtac attgacagaa tcaattccaa tcaactggat gtttcgcaga
     9781 ataaaaggca agacagtcgc gtttagtttg attcccccag cattgccaca taaagctact
     9841 gcaccgttat aagagatcaa tggaagaaga ctgcttagct gctcgccacc tactgtgtcg
     9901 atgacatagt cgatttgctg ttttcctaaa gcttttgttg tctctggcag aaattcatca
     9961 ggtcgtacga cttgcaaagc acctagactt tttagccatg tgacagactc ttcttttctg
    10021 gaaaaggcaa tgattgactg gaatcctagt ttatgtaaca gggctatcgc tgtactgcct
    10081 acaccgccag aagcaccagt aacgacaact tttttatcgg aggtcatacc ttgacggatt
    10141 aaagcgttca cacacaacat agcagtaaac cctgctgtac caaggatcat cgcttgacgc
    10201 agcgtcatat tttttggtag aggaactaac cagtctcctg gtactttttg atattggcta
    10261 taaccgcctg gatgggtgac gccaagccca aaaccagtag caagtacaag atcttctttt
    10321 gaaaaacgtg gattgttact ttggacgaca gttccggata aatcaatccc tggagtcatc
    10381 ggatatgttc gtatcacctg accagactcg ctcattgcca atgcatcttt ataattgaca
    10441 tcagaatagg caatttttac caagacttca ttttcagcca actcagtaaa tggcagatca
    10501 ctgattgctg tatggaaaac aggttccttt attacctgaa aagatcgaaa agcgttcatt
    10561 cgtgagacac tccttttaaa attttctttt atcataccat aaagcctact cttttattct
    10621 tgttcttccg taaatttaga caaaaattag gtgaaaaata aagaatcaaa caactagaat
    10681 ttattccaaa agataaagaa atattcagca aaaaaaattt tacaaagggt tgcctttttc
    10741 aataaatttc gtatactaat acgagtggta gtaaacagga agtttactat tagaaaactt
    10801 cgattcggag gaaattatgg aaattggtga gaagttaaga aatttgcgca tccaaaagaa
    10861 tttgacacaa gaagaactag gcgaacgcac agatttgtca aaagggtata tttcccagtt
    10921 ggaacgagat ttaagttcac catcaatgga aacattcttt tctattcttg aagtcttagg
    10981 tgtgactcca gaagaatttt ttcatcaaga agcagcgaat ctgcaagtgg tttattcaaa
    11041 agaagatcat acggtttatt tcgatgaaga aaacgggtat gaattggaat ggctcgtggc
    11101 tgattcgaat gaaaaagaaa tggagcctgt cttgctgact tttgaaggtg caggagaata
    11161 taagacgttt gaaccttcat tatctgaaac gtttattttt gtattagaag gggaattaga
    11221 gttaagctta ggagagacgg tctatgtggc aaaagcagga cagtcgatct attatcaagc
    11281 gacaaaacaa catcaattaa ggaatcatct caaaaaacga acaaaagtat tgattgttgc
    11341 aacgcaatca tatttgtaat atagggggta aaaacgtggg gaaaccaatc atttcatttg
    11401 atcaagtagt gaaacgctat gatgatgaaa cagtattaaa aaaagtaagt tttgagatcg
    11461 aacaaggaaa attttataca ctgcttggtc cttccggctg tggtaaaaca acgatcctac
    11521 gtattatagc tggattcacc gaagctacag aaggggacat ctattttgaa gggaaacggt
    11581 tgaatgattt gccagctaat aagcgacaag taaatacagt cttccaagat tatgcacttt
    11641 ttcctcatat gaatgttttt gataacgttg cttttggatt gaagatcaag aaaatgtcca
    11701 aatcagatat cgaaaagaag gtaaaagatg ctttgcgtat ggtccagctt cctggatacg
    11761 aaaaacggga gattagtgaa atgtctggtg gtcaaagaca gcgtgtagcg attgcacgag
    11821 caattgtaaa tgaaccgaaa gtattgctat tagatgaacc gttatcggcg cttgatttga
    11881 aactacgtac agatatgcag tatgaactgc gggatctgca acaacgatta ggaatcacgt
    11941 ttatttttgt tactcatgac caagaagaag cgttggcaat gagtgatgag atttttgtca
    12001 tgaataaagg gaaaatcgtc caaagtggta cacccgtaga tatttatgat gagccaatca
    12061 atcatttcgt tgctgatttt gttggggaga gcaatatcgt ggacggtgta atgatcgaag
    12121 ataatctagt tgagtttgtt ggtaagcaat ttgaatgtgt cgatggcggg atgcgtctaa
    12181 atgaaccagt tgaagttgtt ttacgcccag aggatttgac gatcacgaca ccagataaag
    12241 gaaaactagt cgtaacggtc gatactcagc tgtttcgtgg tgtccattat gaaatcatct
    12301 gttatgatga acagcaaaat gaatggatgg tccattcaac gaaaaaagct aaagaaggaa
    12361 gcaaagtagg attggccttt gagccagaag atatccatgt catgcgtttc aacgagtctg
    12421 aagaagaatt cgatgcacgt cttgacagct acgaagaata ggggggacaa gaatgaaaac
    12481 aatgcgcagg atctattccg tcccttatat tttgtggctc ggtttattcg tgatcgcgcc
    12541 tgtattaatg atcatttatc aatctttttt tgatatgaat gggcaattca cgctgaataa
    12601 ttatctatct tattttacgt cggggacatc gctttctatg acgatcaatt ctgtatggta
    12661 tgcatttttg atcacgtttt tcacgctgat catcagttac ccgactgctt attttttaac
    12721 aaaactgaag cacaagcaat tatggctgat gctgattatt ttgcctacat gggtgaatct
    12781 gctgcttaaa gcttacgcat ttattgggat tttcagtgtg cacggcagcg tgaatcattt
    12841 ctttgatttt atcgggatcg ggccgaaaca gatattattc acagacttca gttttctgtt
    12901 cgtggcaacg tatatcgaga ttccatttat gattatgcca attttcaatg cattggaaga
    12961 gatgaatcct tctttgatca gtgctagtag agatttaggc gcgaatagct ttgagacctt
    13021 ccgacgagtg atttttccgt tatcattaaa tggtgtgaaa agtggtgtcc aagcagtatt
    13081 tattccttct ctttctttat tcatgctgac tcgtctgatt ggcggaaatc gggtgatcac
    13141 tttaggaacg gctatcgaac agcatttctt ggttacacaa aactggggga tgggttctac
    13201 aatcggtgtg attttaatca ttgctatgtt catcgtgatg ctcctgacag gcgagaagaa
    13261 aaaaggaggt cgctcataaa tgaaaaaatt taaatggtcg tatctttatt tagggatcgt
    13321 ctttttattc ttatatgcac cgatcttcta tctgattttt tattccttca atgaaggagg
    13381 aacgatgaac agctttacag gatttacttg ggaaaattat gaagctgttt ttgaagatac
    13441 acgtttgatc attatcgtat tgaatacgtt catgctggct ttcttatctg cattgatcgc
    13501 cacgatcatc gggacatttg gggctatggg aatctattac acgaaaaaac gacgtgcacg
    13561 gacagcgttg ttgagcatga ataacatctt attagtctct cctgatgtta ttatcggtgc
    13621 tagtttcttg attttcttta cactactagg attcggatta ggttttcaga gtgtattgct
    13681 ttcccatatt gctttcagca ttccaatcgt cgtcttgatg gtcttgccaa aattgcagga
    13741 aatgaacgat tcaatggtcg atgcggctag agaccttggt gctaataatt tccaagtagt
    13801 caaaaatatc attttgcctt atctttctcc aggaatcata gctggatatt ttatggcatt
    13861 tacctattct cttgatgatt ttgcggttac atttttcgtc acaggaaatg gctttacgac
    13921 gctctcagta gagatctatt ctcgtgcaag acaaggaatc agtttagaaa tcaatgcgct
    13981 aagtgcgtta gttttcttgt tctccatgat attggtcatt ggatactact ttattagtaa
    14041 agaaaaccgt ccaaaacggc tgaagaaaaa cagaagagta cgcgaggggg tggcacggct
    14101 gcgatgaaaa aattacaatc tctgttttta ggtattctca tcattattgc ggtgctgctg
    14161 cttggtgttc gtcaacttga aaaaacaagt ggcatgagtg gagcaaaggt acttaccatc
    14221 tataactggg gagattatat cgatcctagt ttgatcacga aatttgaaaa agaatatgga
    14281 tacaaagtga attatgaaac atttgattct aatgaagcga tgttcacaaa aatccagcaa
    14341 ggtgggacaa actacgacat cgctatacca agtgaatata tgatccaaaa aatgatcaaa
    14401 gaaaaactag tacttccatt agatcattcg aagatcaaag gattaaataa tattgatcca
    14461 cgatttttag acttggattt tgatccagat aatcagtatt cgattccata tttttgggga
    14521 actttaggga tcgtatataa cgataaattc tttgatgcaa atcaaattaa gcattgggat
    14581 gatttgtgga agccggaatt aaaagacagt ttgatgctga ttgacggtgc acgagaagtt
    14641 atggggcttt ctttgaatag tttaggctat tctttgaata gtaagaatat ggcacagctg
    14701 acagaagcag caaataaatt gaatcattta acaccaaatg tcaaagcaat cgtggcagat
    14761 gagatcaaaa tgtatatgat caatgaagaa gcctccgtgg cagtgacatt ttctggcgaa
    14821 gctgccgata tgatgtggga aaatgaacat ttacattatg tgatcccacc agaaggatcc
    14881 aatctttggt ttgataatat cgttatgcca aaaaccgtga aaaataaaga aggtgcctat
    14941 gatttcatca actttatgct agaaccggaa aatgcggcac aaaatgcaga gtatattggt
    15001 tattctacac caaatgaaga agcaaaagcg ttattgccaa aagacatttc gtcggacgaa
    15061 caattctatc catctgatga cacgatctcc catcttgaag tctacaaaga tctaggatcg
    15121 aaatacctag gcatctataa tgacctattc ttagaattca agatgtatag gagataatgg
    15181 caaaactcag taagaatggg gacttatcaa cgttctaaag agctaaaaat cgtccttttt
    15241 ctatctgagt tttccaatct tctaccaaaa gcggatgaaa cgccgctttt tttgtgttat
    15301 tttatttttt ctaccacgtt tttctaccag agaaaagtgg acaaaaccat tgtaaaatag
    15361 ggcccgagac gggctagtaa atctaagatt gaaaaagtta cagaaggata aagtaatt
//
LOCUS       DACEZN010000057        15190 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEZN010000057 DACEZN010000000
VERSION     DACEZN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.057"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..436
                     /gene="dnaI"
                     /locus_tag="IXH60_001399"
     CDS             <1..436
                     /gene="dnaI"
                     /locus_tag="IXH60_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1990334.1"
                     /translation="GSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKD
                     QVAEKLDAVKRSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDE
                     LEQHLSVTQRGDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            565..1005
                     /locus_tag="IXH60_001400"
     CDS             565..1005
                     /locus_tag="IXH60_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1990335.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            1149..1850
                     /locus_tag="IXH60_001401"
     CDS             1149..1850
                     /locus_tag="IXH60_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1990336.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            1847..2968
                     /locus_tag="IXH60_001402"
     CDS             1847..2968
                     /locus_tag="IXH60_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1990337.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            2983..4215
                     /gene="pepT"
                     /locus_tag="IXH60_001403"
     CDS             2983..4215
                     /gene="pepT"
                     /locus_tag="IXH60_001403"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1990338.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            4347..5255
                     /locus_tag="IXH60_001404"
     CDS             4347..5255
                     /locus_tag="IXH60_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1990339.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            5290..5562
                     /locus_tag="IXH60_001405"
     CDS             5290..5562
                     /locus_tag="IXH60_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1990340.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            5573..6619
                     /locus_tag="IXH60_001406"
     CDS             5573..6619
                     /locus_tag="IXH60_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1990341.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            6870..9479
                     /gene="clpB"
                     /locus_tag="IXH60_001407"
     CDS             6870..9479
                     /gene="clpB"
                     /locus_tag="IXH60_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1990342.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(9630..10301)
                     /locus_tag="IXH60_001408"
     CDS             complement(9630..10301)
                     /locus_tag="IXH60_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990343.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(10294..10788)
                     /locus_tag="IXH60_001409"
     CDS             complement(10294..10788)
                     /locus_tag="IXH60_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1990344.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(10808..12028)
                     /locus_tag="IXH60_001410"
     CDS             complement(10808..12028)
                     /locus_tag="IXH60_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1990345.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            12175..14010
                     /gene="lepA"
                     /locus_tag="IXH60_001411"
     CDS             12175..14010
                     /gene="lepA"
                     /locus_tag="IXH60_001411"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1990346.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(14104..15186)
                     /locus_tag="IXH60_001412"
     CDS             complement(14104..15186)
                     /locus_tag="IXH60_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1990347.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4925 a         2772 c         3170 g         4323 t
ORIGIN      
        1 aggaagtttt ggtattggaa aaacatattt attaggagca acagcgagag aattagcgat
       61 tgctggattc accacgacgc tagtccattt tccgacattt gccgttgaga tgaaacaggc
      121 aatcggaaaa gatcaagtgg cagaaaagtt agatgcagtg aaacgttcac ctattttgat
      181 gatcgatgac attggtgcag atgcgatgag tagttggata cgagacgaag tattcggtgt
      241 tatccttcaa tatcggatgc aggaacagtt accaaccttc ttctcatcga attttacaat
      301 ggatgaattg gaacagcatt tgtccgtcac tcaacgtgga gatgaagagc cgctaaaagc
      361 gaaacggata atggaacgca tacgttattt gacaaaagaa atcgaaatga ctggtcgcaa
      421 ccgacgcaat ggttgaacag ttgacaagtc caactgacat ttgacctagt agtaaaagca
      481 ggtcagcgaa tcattatatg gacagaaatg accggagaca atcaccgcaa caagtaatgc
      541 agaaacttaa acggaggatt ttttatgagg gctatttttt ctagtatatc tgtagttggt
      601 ctattttttc ttttagcagg ctgcggtagc gcagatagca acacaggttc tgttgattcg
      661 attgatgcgg gtcaaacagt aaaaacaagc agtacgaaaa cggagagaca aagcatattt
      721 gtcggaacga ttgaaagtat tactcaagta gaagcagaag aagaaacgct tcagttgaaa
      781 ctcgtcgatg tcatggccaa agaagatcca gcgaatatgg gaacgtcatt cagcaatgac
      841 ggagtgatct taaatgctac tgctgaaaaa ataagcgtgg acataaatga attgaaaatc
      901 ggcgacaaag tccaatttac attagttgag atgccgatca tgacgatgtc tatcccaccg
      961 caagttcctg gagattcgat tattgatgtt tcattgaaca aataaccaat aacatttttt
     1021 caaaaataag aaaatgttgt tttatagaac gaaaaggtat attttttaag gagatatggg
     1081 aattttttta ttcctagtct ccttatttta tttggaaaat gctataatta tctagaagga
     1141 gtgaagctat gaaccattta gatttatctc agcgcttagc cgcagtgggt gactttgtcc
     1201 ctgaatccgc tcgtttagca gacattggat cagatcacgc ttacttaccc gttgcattga
     1261 tattaagagg aaaaatcgaa tatgcagttg ctggtgaagt cgttcaaggt ccttatgaat
     1321 cagcacgtaa gcaagtcaaa aaagatggat tagaagaccg tatcaccgta cgccttgcaa
     1381 atggtctaga tgctatccag ccacaagatg gtattacagc tgtcacgatt gcgggtatgg
     1441 gaggttcact gatccgagat atcctagaag ctggcaagtt gaatcaacgc ttaagcggac
     1501 aagaacgttt gatccttcag ccaaacatcg gagagcgtac attacgatct tggctgcaag
     1561 aaaatcagta taaaatccaa tcagaaaaaa tacttgaaga aaataaaaaa atatatgaaa
     1621 tcattgtagc cgaaaaagaa caaaaaccag ttacttacaa tgaaatggag ctgatgttcg
     1681 ggccatttct gcttgaagag aaaagtgaga tcttcaaatc aaaatggaca agagagcttc
     1741 aacaaagaaa agctatccgt gaacagctca aacaagcagc taatcaagag gcacgtcaaa
     1801 aacaaatcga gcaggaaatc caaatgattg aggaggtttt aaagccatga caatcagcgg
     1861 acgcactttc atccaaaagt ttgaaagcta ttgtcctcag tggctagctg aagaaggaga
     1921 cccagtcgga ctgcatatcg gaacattgga taagccaatc caacgagtaa tgatgacatt
     1981 agacgttcgt ccagaagtgg tcgaagaagc aatcaagaaa aaaatcgatt tgctgattgc
     2041 taaacatcct ccaatctttc gtcctgtaaa acggctggtt actgatcagc cacaagaaaa
     2101 aatgtatgca gatttgttga aacatgatat tgccgtctat gcagcacaca cgaacatgga
     2161 tattatttgg gatggactga atgactggtt ttgtgaactt ctcggtattg aagtggagag
     2221 ttatttagta aagactcatg agattcatta taaaaaactg gccgtttatg tacctgtgga
     2281 tcatgcacag aaaatgcgtg aagtattagc tgcagctggt gctggtacac aaggtgatta
     2341 taccggtaca agttttacct cgattggaca cggtcgattt actccggaag ctggtgcaca
     2401 accagcgatt gggaaagtag ggaagacaga acaagttcaa gaggcaaaag ttgaggttat
     2461 tttgccggaa actatcgaaa aacaagtgat tcaagccatg cggtcagctc atccttatga
     2521 ggaacctgct tatgatcttt tcgctataga tgaaccagtc gaaatgtttg ggttaggacg
     2581 agtaggtgaa ttgccgcaag aaatttctat agaggcattt gtagaacaag taaaagaagc
     2641 gttccaacta gatggactga gaatagtcca gccaaaaaat gcaaaatcct ctgtgaagcg
     2701 cattgcgatt tgcggtggaa gcggtgaaaa attttatccg caagcaatcg cccaaagagc
     2761 agatgtctat atcacgggag atatctatta tcatacggct catgatatgc aaagcgctgg
     2821 gttgattgca attgatcccg gtcattatat tgaatcgtta tgcaagcaac gatttatcga
     2881 gaaatttgaa tcttggaaac aagaagaaaa ttgggatatt aactttttcg tttctgaaac
     2941 aaatacaaac ccatttcaat ttaattaaaa ggagagaata ctatgtacga aaatcttttg
     3001 ccacgttttt tacgatatgt caaaacagaa acacgctccg atgcaacaag caaaacgact
     3061 ccatcgactc agacacaagt tgcttttgcg caagaattga aaaaagaatt agaagagtta
     3121 ggcttagctg atgttttcta taatgaagat aacggattcg ttatgggaac attaccaagc
     3181 aatgttgatc atgaagtgcg ttccattgga tttatttccc acatggatac agcagatttt
     3241 aatgcagtag gagtcaatcc acaaatcatt gaaaactatg atggcgagtc tacgatcgta
     3301 ctagatgcgg aagaacgcta tacattgaat acaaaagatt ttccaaattt gaaaaattat
     3361 gaaggccaca cattgatcac aacagatggc agtacattac tgggagcaga tgacaaatca
     3421 gggattgcgg aaatcatgac tgcaatggag atcttgatca ataatccttc tatccctcac
     3481 ggtaccatca aagtggcttt tggtcctgat gaagaaatcg gtgtcggagc ggataaattc
     3541 gatgttgagc aattcgatgt agattttgct tatacagtcg atggtggtcc tttaggtgag
     3601 ttgcagtatg aaacgtttag tgctgctcaa gcaaatatta cgatccaagg gaaaaatgta
     3661 catccaggga cggcaaaaga tacaatgatc aatgctctac agttagcaat cgattttcat
     3721 aatcagcttc cagccgatga agttccagaa aaaacggaag gatacgaagg tttcttccat
     3781 ttgatggcat tggatgggaa tcctgaagaa gcttctatga gttatatcat tcgcgatcat
     3841 gatcgtgaaa aattcgaagc acgcaaagca caaatcacac atatccaaga agagttgaat
     3901 cagcgatttg atcaagaacg aatcaaagta gatatgtatg atcaatatta caacatgaaa
     3961 gaaatcattg aaaaagatat gagcatcgtg gaattagcga aacaagcaat gatcgagctt
     4021 ggtatctctc cagtgatcga accagttcgt ggaggaacag atggttcaaa aatctcttac
     4081 atgggtattc cgacgccaaa tctatttgcc ggaggagaaa atatgcacgg acgattcgaa
     4141 tttgtttctt tacaagtaat ggaaaaagca acagacgtga ttgtaaaaat tgcagaattg
     4201 aacacaaaga gttaaaatcg aaaaagaaca gcttatgaca aggcgttcgt cccaatgatg
     4261 gaatgtcatc tgtctaaggc taaagggcag gacaatggaa ttggttgtcc cgccctcttt
     4321 tttgaagagt aaataggagg taaagaatgg aaaagctgct agttttttat aacaaaagtt
     4381 cagggaaaga tgagggtgag aaattagcac gctggtttca agattatgta aaagaaaagc
     4441 agccagaatt atatgtagaa ctagctgaaa ctggcccatc gattgatgat gcggaattag
     4501 tcaagaaagc agaagccata gaagctgata ctttagtaat tataggtgga gacggcacga
     4561 ttcatcatat cgtccaagcg ttccaaaaaa cattatctca ataccatgtc ggattacttc
     4621 caggtggaac tgtcaacaac cttgcacgtg ttcttgagat tcccctcaaa aaagaagaag
     4681 ctgctgacgt gatcctggaa ggcaatgtac ggaaaataga ctatggtcaa gtcaatgatg
     4741 aagtcattat ctctacactg acaatcggaa tcttagcaga tacagcagtt tggatcagtc
     4801 aagaagaaaa acagcgttac ggctcgtgga tctttatcaa gaagtttttt aagttattgc
     4861 tgaagaagaa aaaatatcat ttggatttag aaacagacga ggagcattgg aaaggaaaat
     4921 cccaactgtt gacagtcacg atgtctaact ccgtgggcgg ttttacaaat tttgacgact
     4981 cagcgaccct agatgatgga aaatttcatg taacgatcgt accttcctta gatattttcc
     5041 gatttatttt ttatttgcct agaatcatga gaggcaaatt ctatacgatc cccggcatta
     5101 cttatttcac agccaaacgc atgaaaatcc aagcgcagga aaaatctgtg ggcactcgaa
     5161 cggatggaga cacgacagat gatcttccag ttgaattatc tgttgtatca aaaggattaa
     5221 cagtttattc accaaagaaa gaagaaaaag cttagaaata gtagttaaca gaaagttgga
     5281 agaagagtaa tggatgtatt gttacacaat cttttgtggt tttacctttt cattgtgaat
     5341 ttttacttat tttgtctgat ggggtatgat aaacatcagg ctagaactag cggctggcgt
     5401 gttcctgaga ataatttgat atttgtcagc ctgttaggcg gtggtttggg aggattgatc
     5461 tctcgaaaag ttttccatca taaaactcgg aaaaagaagt ttactatagg attcgctgca
     5521 ggtgtacttg ttgatttgat tctcatttat tttttccatt aaggagcagg agatgtcgtc
     5581 taaaggaacg aaaactaaaa tcattctgaa tctgcttttg ctgattgtga tttttgccat
     5641 tatttattat ttaatcaatc aatcatttgc agatatcttt gccgaattgc tgtctaccag
     5701 tgttgttgtt ttggcagcgg ctgttatatt agggacagtt taccaaatcg ctgaagggcg
     5761 ctcaatcaaa gaaattgctg aacctttcgc gccgaatttt acaacgaaag atgggttctt
     5821 gacctctagc tatattgcgt tttaccgaat cgtttcgttt ggtacaggaa cgctgatctc
     5881 ggagatttat ttttataaca aaaaaggaat gaagctatcc cagggagcag gggtgacagc
     5941 actccatatg attatgtaca aactggcagt gatcacttat gcattagctg gcttggtcat
     6001 ccaattctct cttttttatt ctaatggtcc atcaatgatc tggtttatca tcgctggtat
     6061 ccttttgacg atcgtgattg ttgccttttt attggcgatg tccgtcagct tgaatgttca
     6121 aatatttttt gtcagcttat cgaataagtt atttaaaagt gagcgtttgc ggaaatgggt
     6181 ggatacatgc aatcagcaga tctactcttt aagggaaacg gtccaaacca ttatccaaga
     6241 caggactgcg ctattgcgga tttatctatg gaatatgtta aaacttgtat tttggtatgt
     6301 acttccttat ttgattctga ttgaaaacca tcctaaaata gattttctac tgaccatttc
     6361 gtttatcagc tttgcagtag tgctttcagg ggttattcct acgcctgctg gtattggttc
     6421 ctttgaattt gtctatttgt tattattcag accagttgtt ggaacagttg acgcagtttc
     6481 ttcgctttta ctgtatcgtt tcagttcctt tatcttgcca tttctctatg gatttgtgta
     6541 tgtgattgct gaacggcgaa gagtgatcaa agaagaaata atagaagtaa aaaaagagaa
     6601 taaagaaaat caagggtagg tcttttataa gatctaccct ttttatcaaa tcaaagaaaa
     6661 acgttatgga taagataatt ttacgtttgt ttggctcttt ttttatatac atacaatcaa
     6721 ttttgttttt ttaagattta tataagtcga gaaaaaaagt caaaaaacca tgtgtatatt
     6781 ttgaccaaaa ttgaccaaag cgatataata gtttttgtaa agatcaaaga aataagtgat
     6841 cttaccttta attaggaggg tatacctgta tgaatattga gaaaatgact acgacgcttc
     6901 aagaagcgat tgctgaagct caacagattg cggtcactcg aaaacatcaa gatatcgaca
     6961 ttgcccatgt atggaaaatc tttttacaac ctaatcattt tggtcgaaat ttctatacgg
     7021 atgccggatt ggatgtcgaa tcttttgaac atgaaattga tcgtctactg gatgagtatc
     7081 cagttgtttc tggtggaaac gtccaatatg gacaaaacct cagccagaat ttctttcgat
     7141 tattgaatga agcagaccaa atacgtgaaa gcttcggcga cgagtttctt tcaacggaag
     7201 tcgttatttt agcgttgatg aaattaaaaa attatccgtt aactgtttat ttgaataaaa
     7261 acggattatc tgaaaaagaa ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag
     7321 tgacctcaca aaaccaagaa gaacaataca aagcattaga aaaatacggc gttgatttag
     7381 tacagcaagt caaaagcgga aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg
     7441 atgtcatccg tattctttca cgaaaaacaa aaaataatcc tgttctgata ggagaacccg
     7501 gtgtagggaa aacggcaatc gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc
     7561 ctgaaaattt aaaagataaa acgatttttt cattagatat gggtgcattg atcgctggag
     7621 caaaattccg tggtgaattt gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa
     7681 gtgatggacg tatcctcttg ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa
     7741 cagaaggaag tatggatgcc ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac
     7801 atttgatcgg tgccacaact ttagatgagt accgtcaata tatggaaaaa gacaaagcgt
     7861 tggaacgtcg ctttcaaaag gtattagtca aagaaccaac agtggaagat acgatcagta
     7921 ttctgcgtgg attaaaagaa cgttttgaga tccaccatgg tgttaacatc catgacaacg
     7981 cattagtagc agcagctact ttatctgacc gttatattac tgatcggttc ttaccggaca
     8041 aagcaatcga tttgatcgat gaagccagtg caacgattcg ggtagaaatg aactctatgc
     8101 caacggaatt agatcaagtt actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac
     8161 tgaaaaaaga atcagatgat gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag
     8221 ctgaacttcg tgaagaagca aatgcgatga agatgcagtg ggaaacagaa aaagaagaag
     8281 tcaattctgt ttcagcaaaa cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg
     8341 cagaaaataa ctatgattta gaacgcgctg ctgtattgcg tcatggtacg atacctcaat
     8401 tagaaaaaga attgaaggaa ttagaagcta aagcaaaaga cagcgagatt aagatggttc
     8461 aagagtccgt taccgaaaat gagattgctc aagtcgttgg cagattaacg ggtatccccg
     8521 taacgaaact tgtagaaggt gaacgagaaa aactaattaa attgaatgaa acacttcata
     8581 aacgagtgat tggtcaagat gaagcagttg atgcggtcag tgacgcggta attcgttcac
     8641 gggctggatt gcaagaccca aatcgtcctc taggttcttt ccttttccta ggaccaactg
     8701 gtgtcggaaa aaccgaatta gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc
     8761 atatggtacg gatcgatatg agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg
     8821 gagctcctcc aggctatgta ggttacgaag aaggtggtca attaacggaa gctgtcagac
     8881 gaaatcctta tacaatcgtc ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca
     8941 atatcttgtt acaagtccta gatgacggac gcttgactga ttcaaaaggg cgagtagtag
     9001 atttcaaaaa tacggttctg atcatgacca gcaatatcgg ttcacagtta ctgctggaag
     9061 gcgtgactgc tgatggaacg attccagaag cagtggctga acaagtcaac acgctattac
     9121 gtggaaactt caaaccagaa ttcttgaacc gaatcgacga cacgatttta tttacaccat
     9181 tgagcctaga caacgtgaaa ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc
     9241 tggaacatca agaaatcctg ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa
     9301 atgcttatga accagcatat ggtgcaagac cattgaaacg atttatcact aaggaagtag
     9361 aaacaccact agctaaagaa atcgttgcag gacatgttat gcctaaatca aaagtaacga
     9421 ttactttact tgatggacaa cttcatttca aaacagaaga gctagaagaa atcgtctaat
     9481 cttccattga gactataaaa gaaaaaaggg gtctgacaca actagtgtca gacctccttt
     9541 tttcaaataa acagtgttcc agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa
     9601 gctgcccgct tctgtcacaa cctcttttat taggtatttt tcactttttc taatttataa
     9661 ctttcaaagt aacgctcatc ttttttactt tgagaccatg caaacaatcc cattaaggca
     9721 gcacctatcg tatttgtcag caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca
     9781 aacagtgtgc catcagaagc agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat
     9841 aagaattccc agaactccca aaacacgccg caaagtccag caaacgcaaa gccgaataag
     9901 aggaacaacc aaggactggt ttttgatatc tcagcatctt tcatcaagta accaatcaat
     9961 ccatagccaa gagctgtcag gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa
    10021 gagatgatac tgatcaaatg catccctgtg cctaaaaaga cagagatgaa caagaaaaac
    10081 cagtaaaaca aaacgattgc tgctggtatt ttaagtttga aaactttcaa aatgacttgt
    10141 ggcatgaaaa tcaatgcaat ccctaataaa gattccacga taaatgtgat tccttgagaa
    10201 gtcgaatatt tttgtgtgat aaattcataa aaattatagc agacaacgaa tacaccaaaa
    10261 gcaagtaaag ctatttgcac ctttttgatt tctttaatca ctgaaagtcc ctccatctaa
    10321 aaatcttttt acttctgaaa gcaactcaat ttctgaagaa accacttttc cgttcaattt
    10381 gatcaaacct attgtatata agttcaggta gtgaaattga ttttcagctg tttcttgtag
    10441 tgcagcgatt tttttcgggt gggttccgcc ttgctgtcga gtatcagtat ataagccgat
    10501 cactgggatg cctttcgcat aagccacacc gatttccgat gctaccccgg catcgatcgt
    10561 cagcccatct agtaaagcaa tcattaaatc actagccaac actttttctg tgtcagcaag
    10621 agcaatcatt cgactgtctg cataagcagt tttgtcattg attgcttcat tttcttgagg
    10681 aagatagatc gaaagctcag aagagagctc tctgatttct ttaactaact gttggttata
    10741 taacaaatca cttttagcaa acattggtgc ggcaaaataa attttcatct aaacatcatc
    10801 ctttttatca aaaataatcg ctaaagtaag cttccatgtt tggaatgata cgctctagtg
    10861 acttcagtgt ttcttcatct gtttctaacc gttcgattct tgccaaataa gaagcacggc
    10921 gataattcat catcaagcaa gtcaatgttt ggatatccag tcgaacggat tttcctatag
    10981 gttcatttaa gacacggact tgtccctgtt catcccatgt taaagcgaag acaccattat
    11041 tccaaggagc gacaggatct tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg
    11101 gaaaccgttg aaggaattct tttacatcaa cgatccgagc cataaaatat ggttcgatct
    11161 gctctttgat ctgactatct tcaatcaaaa atgctaaagg ctcattttta taaatatctc
    11221 catgtaccca gtagatcatg gaaaaatgtg cggaaataaa gttccataag ccatttcgag
    11281 cttcttgatc aagatagaac atttctttta cgtgaaatat ttcttcagct acccagtaga
    11341 acagaacacc tcttggttca tggttgctat cgtagtagac agcggctgtg cgctcatctt
    11401 cattttcgaa acgccaatac tcttcccaat gaaaatcact gcggaataaa gcaccatggt
    11461 tttgttgggc aaatttgtca taaacggaaa atacgtctgg atgatcaact ggtaatcgct
    11521 caaccatacc acttactggg atttgtttag gtagctgtgt atctctgatt ttaaaggaaa
    11581 gtttatcaga catgatttcc catcctttac gccgatagta ggggatactg taaggataca
    11641 aataagagat ccattgctta ttcttgcgca tattatccaa tgcaacgata attagatctt
    11701 gcatcaatcc atggttagca tattctggat aagttccaac tccagtcaca ccacccattt
    11761 tataacgtgc accgtgaata ttgacttcac aaggatagat agcgatttga gagatcagtt
    11821 tattttcgtg gaaccatccg aacactttcg atacttccaa aatcggtttt ttggaacgga
    11881 taaaagctcg tttattctca aacccgcttt cctcgatgtc tgcttcagtc acttggaaca
    11941 cataggaaag caattcattg aattgatcga tatgttcttg agtgaccggt tttaattcaa
    12001 gattttctct aaaatctttt tgatccattt tttcatctcc atttattagc ttgataacag
    12061 tatagcaaaa ttctacaaaa aaaacttcta tagctgacta attaaaaact ctttaaatta
    12121 ctgcttttcg ttggtataat ggaaagtgct gattatatga aaagaggaga cgctatggat
    12181 ataaagaaaa tgaaagaacg ccaagagcag attcgtaatt tttcgatcat cgctcatatc
    12241 gaccatggga aatctacatt ggccgatcgt attttagaaa tgacgcacac tgtgacgtct
    12301 cgcgaaatgc aagatcagct gttagactcg atggatttgg aacgtgaacg tggaatcacg
    12361 attaaattaa atgctgtaga actcaattac acagcgaaaa atggagaaac atatatcttt
    12421 cacttgattg acaccccagg gcacgtcgac ttcacctatg aagtctctcg aagccttgct
    12481 gcctgtgagg gtgccgtttt agtggtcgat gcagcacaag gaatcgaagc acaaacattg
    12541 gccaatgttt atttagcgtt agacaatgat ttggagatcc tgccagtcat caataaaatc
    12601 gacctacccg cagcagatcc cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc
    12661 gatgctagtg aagctgtatt agccagtgcc aaatcaggga ttgggattga agatattctc
    12721 gagcagatcg tggaatatgt tcctgcacca agtggagact tagatgcacc gctaaaagct
    12781 ttgatctttg actctattta tgatagttac cgcggtgttg ttctaaatgt ccggatcacg
    12841 gacggtatgg tcaaacccgg agacaaaatc aaactgatga gtaatggcaa aacatttgat
    12901 gttacagagg tcggggtctt ctcgccaaaa gcagttgcta gagacttttt gatggtcggt
    12961 gatgtcggtt atatcacagc aagtatcaaa actgtccaag atacacgagt aggggataca
    13021 gtaacgttag cagacaatcc tgccgctgaa gctttaccag gttaccgaaa aatgaatccg
    13081 atggtttatt gtggtcttta tccaattgat acttctcgtt acaacgattt gcgggaagct
    13141 ttagaaaaac tgcaattgaa tgatgctgct ttacaattcg aaccagaaac ctctcaagca
    13201 ttaggttttg ggttccgctg tggattcctc ggacttcttc atatggatgt cgtccaagaa
    13261 cgcttggaac gggaattcaa tctagaattg atcacgactg caccatccgt tatttaccat
    13321 gttaataaaa cggatggtac gactgctaca gtagataacc cggcagattt tcctgaacct
    13381 gtaacgattc aggatgtaga agaaccattc gtcaaagcac agatcatggt cccaaatgat
    13441 tttgtcggtg cagtaatgga actttctcaa cgaaaacggg gagaattcat tacgatggat
    13501 tatttagatg attaccgagt aaatgtcgtc tataatattc cgctttctga aattgtcttt
    13561 gactttttcg acaaattgaa atcaagcacg aaaggctatg cctcattgga ttatgaaatg
    13621 tctggttatc aaaaaagcaa gttagtgaag atggacatct tgctgaatgg agaaaaagtc
    13681 gatgcattaa gctttatcgt tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt
    13741 gaaaaactga aaaaactaat cccacgccaa caatttgagg tgccaatcca agcagctatt
    13801 ggacaaaaaa tcgttgcccg ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa
    13861 tgttatggtg gagatgtttc tcgtaaacgt aaactcttag agaaacaaaa agaagggaag
    13921 aagcggatga aacaaatcgg ctccgtcgaa gttccgcaag aagccttcat ggccgttctg
    13981 aaaatggacg aggacgaacc gaagaaatag caagggtttg tttgaaaaca gtgtaaaaaa
    14041 taaaccatgt ttacatggat ttgccaccgg tttgccaccg gtggtttttt tcggtggcaa
    14101 aaatcaagaa ttctttctaa gattttccat gatatcgacc gtttcatttc tcatcttatc
    14161 tgttacatgc gagtaagtat ccatcgtaat tgaaattctg ctgtggccca atcgttcaga
    14221 gatttctttc atttttgcgc cattttcgag aagaagtgta gcatgagtat gtctgagaga
    14281 atggaagtta aaagagaggg agagtgcatt cgatatcctc ctagtattcc atttcactac
    14341 acttggcgta actagctcac cgtcttcctt cgtacatact gcatttgaat caatgtagag
    14401 ctttccatac ttcattcgat tttctaattg ttgtttctta tgttttttca gaattgctag
    14461 caaggtttgt ccaataaaaa tcgttcgatt ggaactgctt gtctttggcg taccatatac
    14521 ccatgcgccg tcattcttta ccatttgttt ctctacagta attgttccat ttgaaaaatc
    14581 gacatcatcc cacgtcagac cacaaacttc gccaacgcgc attcccgtat aaaatccaat
    14641 attcaaagga atatagaaag gatgtccttc aggagtaatt tctagcatat gatcaaagtc
    14701 ctcaagagaa atgattttta gatctttttt agtcgttggt cgtgcttcat attttggtat
    14761 ttttacatac agcataggat tttgcttgat taatccccaa ggataaaccg ccatattcag
    14821 cgcattctta aggacagagt gagtaatagt cattgttttc ttcgagtaac cctttttaaa
    14881 ttcagcattg atgaaatttt gtaaaagagc aggggagaga tccgtaagtt ttttctttcc
    14941 taaataaccg tttatatgat ttttgatggt aaatcggtag ttttcatagg tattgtattt
    15001 tagatttagt ttaacgtatt cctccatcca aaaatcaagg tattgtttta ctcgagtatc
    15061 cgtacctaaa aagtattgtc ctgtttcgtc aatatctgat aaaacttttc gtaaagcagc
    15121 ttcggcctct ggtcgggtgt ctccgccaac tttctccact ttttttcttg agccatcatc
    15181 attgatatct
//
LOCUS       DACEZN010000058        14624 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEZN010000058 DACEZN010000000
VERSION     DACEZN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.058"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            3..815
                     /locus_tag="IXH60_001413"
     CDS             3..815
                     /locus_tag="IXH60_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1990348.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="IXH60_001414"
     CDS             812..1612
                     /locus_tag="IXH60_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1990349.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="IXH60_001415"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="IXH60_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1990350.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="IXH60_001416"
     CDS             3109..4818
                     /locus_tag="IXH60_001416"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1990351.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9388
                     /locus_tag="IXH60_001417"
     CDS             5030..9388
                     /locus_tag="IXH60_001417"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1990352.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9533..10006
                     /gene="rimP"
                     /locus_tag="IXH60_001418"
     CDS             9533..10006
                     /gene="rimP"
                     /locus_tag="IXH60_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1990353.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10029..11204
                     /gene="nusA"
                     /locus_tag="IXH60_001419"
     CDS             10029..11204
                     /gene="nusA"
                     /locus_tag="IXH60_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1990354.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11226..11519
                     /locus_tag="IXH60_001420"
     CDS             11226..11519
                     /locus_tag="IXH60_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1990355.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11516..11827
                     /locus_tag="IXH60_001421"
     CDS             11516..11827
                     /locus_tag="IXH60_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1990356.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11840..14146
                     /gene="infB"
                     /locus_tag="IXH60_001422"
     CDS             11840..14146
                     /gene="infB"
                     /locus_tag="IXH60_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1990357.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14173..14520
                     /gene="rbfA"
                     /locus_tag="IXH60_001423"
     CDS             14173..14520
                     /gene="rbfA"
                     /locus_tag="IXH60_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1990358.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2598 c         3255 g         3789 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5701 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5761 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5821 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     5881 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     5941 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6001 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6061 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6121 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6181 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6241 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6301 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6361 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6421 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6481 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6541 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6601 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6661 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6721 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6781 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6841 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     6901 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     6961 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7021 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7081 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7141 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7201 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7261 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7321 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7381 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7441 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7501 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7561 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7621 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7681 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7741 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7801 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7861 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     7921 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     7981 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8041 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8101 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8161 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8221 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8281 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8341 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8401 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8461 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8521 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8581 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8641 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8701 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8761 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8821 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     8881 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     8941 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9001 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9061 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9121 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9181 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9241 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9301 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9361 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9421 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9481 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9541 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9601 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9661 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9721 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9781 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9841 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
     9901 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
     9961 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10021 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10081 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10141 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10201 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10261 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10321 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10381 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10441 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10501 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10561 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10621 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10681 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10741 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10801 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10861 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    10921 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    10981 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11041 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11101 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11161 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11221 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11281 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11341 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11401 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11461 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11521 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11581 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11641 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11701 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11761 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11821 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    11881 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    11941 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12001 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12061 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12121 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12181 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12241 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12301 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12361 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12421 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12481 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12541 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12601 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12661 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12721 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12781 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12841 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    12901 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    12961 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13021 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13081 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13141 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13201 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13261 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13321 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13381 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13441 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13501 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13561 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13621 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13681 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13741 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13801 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13861 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    13921 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    13981 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14041 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14101 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14161 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14221 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14281 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14341 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14401 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14461 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14521 tcaaaaactc ccgcaaatcc tgtgaaatca ggctctttgt caataaggac tgatgagttg
    14581 tgcaaaatca aatctgagtc agaatgaacc acattctggc tcag
//
LOCUS       DACEZN010000059        14411 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEZN010000059 DACEZN010000000
VERSION     DACEZN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.059"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(220..582)
                     /locus_tag="IXH60_001424"
     CDS             complement(220..582)
                     /locus_tag="IXH60_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990359.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(656..1036)
                     /locus_tag="IXH60_001425"
     CDS             complement(656..1036)
                     /locus_tag="IXH60_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1990360.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1118..2293
                     /locus_tag="IXH60_001426"
     CDS             1118..2293
                     /locus_tag="IXH60_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1990361.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2370..2954)
                     /locus_tag="IXH60_001427"
     CDS             complement(2370..2954)
                     /locus_tag="IXH60_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1990362.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3155..4153
                     /locus_tag="IXH60_001428"
     CDS             3155..4153
                     /locus_tag="IXH60_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1990363.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4265..4807
                     /locus_tag="IXH60_001429"
     CDS             4265..4807
                     /locus_tag="IXH60_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1990364.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQYFNKKRKKTSI"
     gene            complement(4804..5463)
                     /locus_tag="IXH60_001430"
     CDS             complement(4804..5463)
                     /locus_tag="IXH60_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1990365.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5465..6229)
                     /locus_tag="IXH60_001431"
     CDS             complement(5465..6229)
                     /locus_tag="IXH60_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1990366.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6237..6914)
                     /locus_tag="IXH60_001432"
     CDS             complement(6237..6914)
                     /locus_tag="IXH60_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1990367.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6951..8336)
                     /locus_tag="IXH60_001433"
     CDS             complement(6951..8336)
                     /locus_tag="IXH60_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1990368.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8546..8630)
                     /gene="ffs"
                     /locus_tag="IXH60_001434"
     ncRNA           complement(8546..8630)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IXH60_001434"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8653..9168)
                     /gene="tadA"
                     /locus_tag="IXH60_001435"
     CDS             complement(8653..9168)
                     /gene="tadA"
                     /locus_tag="IXH60_001435"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1990369.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9275..10132
                     /locus_tag="IXH60_001436"
     CDS             9275..10132
                     /locus_tag="IXH60_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1990370.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10213..10548)
                     /locus_tag="IXH60_001437"
     CDS             complement(10213..10548)
                     /locus_tag="IXH60_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990371.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10615..11985)
                     /locus_tag="IXH60_001438"
     CDS             complement(10615..11985)
                     /locus_tag="IXH60_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1990372.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12063..12599)
                     /locus_tag="IXH60_001439"
     CDS             complement(12063..12599)
                     /locus_tag="IXH60_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1990373.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12908..14389
                     /locus_tag="IXH60_001440"
     CDS             12908..14389
                     /locus_tag="IXH60_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1990374.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4436 a         2874 c         2346 g         4755 t
ORIGIN      
        1 tatttcttcc ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa
       61 ttattattat tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa
      121 actatcttta gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt
      181 gaaaaaaatt caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc
      241 tacttcgtaa gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc
      301 gcctaattct tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc
      361 ctctttaata tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac
      421 tatttctttt aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc
      481 taacgcaaat tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc
      541 caaatgcaac ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa
      601 cttgttctat ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt
      661 tcttattttc tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct
      721 ccgcaatagt ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat
      781 atggaacagc tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat
      841 attcatcaat gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga
      901 tatttttcgt ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat
      961 caagagaaac gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc
     1021 caattttata agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc
     1081 aaaaaaagaa gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat
     1141 ttcaagaaaa gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga
     1201 tgacttactt cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa
     1261 agcgtttagt gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact
     1321 atatcaggct atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa
     1381 tatcgttttt acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa
     1441 accaggcgat tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga
     1501 aacaaatcaa cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat
     1561 ggacttgtct gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg
     1621 caatcctcac aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga
     1681 attgtgttta aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt
     1741 attcgatcat acatttactt ccatgctaac gattgatcct aatttagatg atctactagt
     1801 tgcttttact tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt
     1861 tatcaaaaat ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca
     1921 cgaaatcaac acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg
     1981 gttaagccaa ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga
     2041 acagtatctt ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga
     2101 ttttaccgcc tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa
     2161 agtcgtatta aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa
     2221 tattgcttgt ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga
     2281 gacaatcgat taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct
     2341 ttttcataat tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact
     2401 acatcatgta caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg
     2461 tccatccctt ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg
     2521 ccacctttat acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc
     2581 atattagctg gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga
     2641 cctgaatttg ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct
     2701 tcaaaacttt cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct
     2761 tggatcatga aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg
     2821 gaaagttcaa tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta
     2881 atgtcaccac ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt
     2941 tgtgggaatg ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata
     3001 atacctaacc tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac
     3061 attctataac ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta
     3121 aattctgcta tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat
     3181 cggtgcaggt cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac
     3241 aaaaatcatt gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa
     3301 atatatttac gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt
     3361 agaaaaacaa ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct
     3421 tactcgtgaa gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc
     3481 agtcatttta gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc
     3541 tgaatcattt gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg
     3601 aaaaaaagtc gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga
     3661 gcctattgca agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca
     3721 tagtgtatca cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg
     3781 attatcaggc aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga
     3841 gacgattgat ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga
     3901 acatctttct tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat
     3961 gtctacttct attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt
     4021 gaaactgatt gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta
     4081 tattaatcca aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct
     4141 tcgtcctagt tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct
     4201 tgcatttttt tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa
     4261 taatatggtc gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg
     4321 tggcgtaact gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc
     4381 tgatttgact ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt
     4441 acataacgcc attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat
     4501 gagtccgttg caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct
     4561 agccttgtca atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga
     4621 caaggtgaaa cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac
     4681 cttcttagat gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa
     4741 agaaccagaa aaaagaagtc gccttactca atatttcaac aaaaaaagaa agaaaacgtc
     4801 tatttagacg agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa
     4861 aacagcaaaa aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc
     4921 ttctggaagt acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt
     4981 gaaaaacgtt tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc
     5041 gaaaacaact ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc
     5101 attcttttgc aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc
     5161 ataatccact aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac
     5221 tgggaaatct ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg
     5281 atcaaaattt ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg
     5341 tccgatatca aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat
     5401 cagtgtcaga aataggctga acaatccagc atattccatc cactgccatc tcgaattttt
     5461 catattagaa gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc
     5521 caggtacatc ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc
     5581 gtatcccagc ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt
     5641 gcagccctaa tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga
     5701 tcggcggcgt ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa
     5761 ggccgcgttc agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt
     5821 gaatagcaag tgttgcttta actacttttt cataggtcag ataattatct aagcctacca
     5881 ctacatagtc aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca
     5941 atcctgcttc tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga
     6001 ttgttgccaa agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg
     6061 ctagacgatt agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag
     6121 gtaattttct tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc
     6181 cacgataaat cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac
     6241 tcttcccttt ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct
     6301 tgttttttta ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt
     6361 tttttgcgat tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt
     6421 tttttctttt ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca
     6481 aaatacgcac aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc
     6541 tgatctggat gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca
     6601 taaccccaat ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca
     6661 agcttttctg cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct
     6721 atcaaaaaat tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta
     6781 ggctcctcaa cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt
     6841 tcagctgatg attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt
     6901 tgttttgcag acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt
     6961 gggtagtgag ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat
     7021 tcattttttc cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg
     7081 gtagtaaaag tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg
     7141 ttaatagaat catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat
     7201 cccattccct tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact
     7261 aagcggatca gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac
     7321 gcagtgtgaa gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca
     7381 ctgtttaata ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg
     7441 ataaacgaat gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct
     7501 ttttctttta ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct
     7561 gtaaaagctg tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc
     7621 tcttcatcga tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct
     7681 atttggacat cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa
     7741 ataacatcaa ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga
     7801 ctcatcaaaa ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata
     7861 tcataaggat tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta
     7921 aatgcaatta gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc
     7981 catgccggtg gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca
     8041 gcatgatcgt ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct
     8101 atcgtcgctg catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt
     8161 gcttctgaca atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc
     8221 gttttttctt tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc
     8281 caattttcta aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac
     8341 cctctcgcct ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag
     8401 ctctatttca gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat
     8461 cagtctggct aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca
     8521 gacccgaaag tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc
     8581 tgcttccttc cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa
     8641 gaatagtata ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg
     8701 gcggagcttt cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc
     8761 ttctacatat gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc
     8821 tgttccgcct tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat
     8881 cgcgccgctg cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg
     8941 ccagcttccc actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc
     9001 atcttgcgtg gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac
     9061 cactgctcca atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag
     9121 gcgcatccat ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct
     9181 gcctttgttt tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt
     9241 ttctatggta aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct
     9301 ttacccacaa gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc
     9361 aattgatgat gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct
     9421 tttgaaagcg ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct
     9481 ttttaaagat gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga
     9541 atcaaaagga gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta
     9601 ttctgctgat ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg
     9661 tgattatctt gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga
     9721 tacgacaacg tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt
     9781 gtttgctgaa gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg
     9841 tactttttct atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag
     9901 taagttgatc aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca
     9961 agaattatgg aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg
    10021 taatactcta aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa
    10081 agccaacgat ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat
    10141 taaaggggtt gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa
    10201 agctagtttc tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc
    10261 ggttgaatct ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt
    10321 tgctaacatt cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag
    10381 taatttttca tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt
    10441 catatgatgg atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc
    10501 agcggctgct acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc
    10561 ctttttatct atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg
    10621 aaatctcaat cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc
    10681 gacaggtatc ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt
    10741 ttctcctgct gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc
    10801 tatctttttt gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc
    10861 tcctaaatgc aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg
    10921 aacttcccaa agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc
    10981 atttgaaaac tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt
    11041 tcgtgtcttc ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa
    11101 atgtccttca ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac
    11161 gcttccccat tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt
    11221 tactggataa ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt
    11281 acaagtcgaa taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat
    11341 ctcttgctga cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt
    11401 tggatctttt cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa
    11461 aaagcccgaa aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga
    11521 aactccccat cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt
    11581 cgtcactaat aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg
    11641 agccgcacac aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat
    11701 cattgcactt ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt
    11761 gacagtgcca agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta
    11821 agtttggacc atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac
    11881 gcttccttgt tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata
    11941 ttttttgata aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta
    12001 gacattttac aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct
    12061 gcctaaggtg actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta
    12121 tcccaaacag cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt
    12181 cccaatgcat taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct
    12241 ttgacatatg agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca
    12301 atgatttcac gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt
    12361 aaaacatcat cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct
    12421 aatatcttct cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa
    12481 caaccgcaga actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg
    12541 tataccatac ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt
    12601 cttacctccg tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat
    12661 tattgataat tatcaaaaca tatttagtta tatccattaa aaatcaattt ttccgcctta
    12721 aaaacaagaa tatcttgaca aaatacccta ggaaataaac aatagaataa acaaggtaac
    12781 aaaaaaatta aataacgtta tataaaaaag aaggcgacac tctgtgagaa taacacagac
    12841 tgaaatgact ttccactcac agtttttgtc taactatata caaaaacaga ataaaaagga
    12901 ggtatttatg ctaattgaag caagaaaaaa agagttactt gttctacaaa tggtcattca
    12961 aaaaaataat tgttctttac aagaattatc caatgactta catattccaa aacggacaat
    13021 caaagaactg atccgaaaaa taaatgtatc actcaagcaa tttttattga ttgatgaatt
    13081 tatttattct aatcataaag gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt
    13141 agctgttttt tctaagttaa aattatgcta cctaaaagaa tccaatcgtt ttaactattt
    13201 gatgttacta atcaattatc cacaaactgc tgtaccaaaa aaatatttac ttgaacaact
    13261 ttatatatcg ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa
    13321 attccattta cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc
    13381 tatccgtctc tatctttact tttttctttc agatacattt ggaggtaacg attggccatt
    13441 ttcctcactt gaattaaatg cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat
    13501 ttcaaaacaa gaaaaaaata tctacttgct gttaacttta ttcatattac gaaacaaaca
    13561 acaggcatat tttccaaaaa cttcttctga aatcactgaa ttgatggact tattgcagca
    13621 tacacatgat tattcagaat actttacttc tttttcagtg agtcgccctt gtgaagagac
    13681 agaaacagag agagcctact ttaattattt tttctattgt ttttttccaa atcaaatctc
    13741 ttttcgacaa aaacaggaga ttggtttttt attttccaca agcaaaaacc ttatttgtcg
    13801 acaagtcggt caattgatga cttacttaac tcaacagttt cctctcaacc atttagaaaa
    13861 taaaatacca atttattttt attttcttgt gttggcagtt gtttttttcg aactgttggg
    13921 tgaatccagc agtagttttt ctacactttg ctttccagaa gccacgtcca atcattctag
    13981 ttcttttata caggaaaaga aattaagaga aaaaatcgat acaatttttt caaaaaatgg
    14041 ggaaagaaac caaatcagtc aataccttag tcaattgatc caacacttac tacaaacaga
    14101 aacgaaagag cagttactca tccatttgca gataaccaaa cagtttacag gaaattatca
    14161 tcttcaaagc catttgcagc agttatttcg caaagatgct gttaaaatta ccgcatattc
    14221 agaacatgct gatttgatca ttacagatca gcatgagaaa atttcagatg aaacggagac
    14281 tttctatctg gatacaacgc acaatacagt gttatttggc aagttaataa aagcggttca
    14341 tgaactgtat atgaaaaaag gttatgagaa caaaacaaag aggatgtgac aaaattcttg
    14401 tcacatcctc t
//
LOCUS       DACEZN010000060        14115 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEZN010000060 DACEZN010000000
VERSION     DACEZN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.060"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..552)
                     /locus_tag="IXH60_001441"
     CDS             complement(<1..552)
                     /locus_tag="IXH60_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1990375.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
     gene            complement(568..1047)
                     /locus_tag="IXH60_001442"
     CDS             complement(568..1047)
                     /locus_tag="IXH60_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1990376.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1053..2077)
                     /locus_tag="IXH60_001443"
                     /pseudo
     CDS             complement(1053..2077)
                     /locus_tag="IXH60_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2094..3200)
                     /locus_tag="IXH60_001444"
     CDS             complement(2094..3200)
                     /locus_tag="IXH60_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1990377.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3429..6026)
                     /gene="adhE"
                     /locus_tag="IXH60_001445"
                     /gene_synonym="adhC"
     CDS             complement(3429..6026)
                     /gene="adhE"
                     /locus_tag="IXH60_001445"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1990378.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6331..6711)
                     /gene="yajC"
                     /locus_tag="IXH60_001446"
     CDS             complement(6331..6711)
                     /gene="yajC"
                     /locus_tag="IXH60_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1990379.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6808..7953)
                     /gene="tgt"
                     /locus_tag="IXH60_001447"
     CDS             complement(6808..7953)
                     /gene="tgt"
                     /locus_tag="IXH60_001447"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1990380.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8171..8869
                     /locus_tag="IXH60_001448"
     CDS             8171..8869
                     /locus_tag="IXH60_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1990381.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9037..11676)
                     /locus_tag="IXH60_001449"
     CDS             complement(9037..11676)
                     /locus_tag="IXH60_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1990382.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12295..13326)
                     /gene="queA"
                     /locus_tag="IXH60_001450"
     CDS             complement(12295..13326)
                     /gene="queA"
                     /locus_tag="IXH60_001450"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1990383.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13519..>14115
                     /locus_tag="IXH60_001451"
     CDS             13519..>14115
                     /locus_tag="IXH60_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1990384.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3957 a         3095 c         2543 g         4520 t
ORIGIN      
        1 gcctacgaga taacctccca taaacaaagc agagatcgaa gcaccaccag caacaagtga
       61 atacatgata aaagcagtac ttggaggaat gaccatacct gtcggagctg acgcgatatt
      121 gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact tgctgaggga tcaacactcc
      181 accaatcgcg gtagaagcgg caatggccga gctagagatg gaaccaaata aagcattccc
      241 taacacattc gtatgagcca aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc
      301 cactagtttc ttagctatcc ctccattatt catgataatg cccgagagaa cgaaaaacgg
      361 aatagcgatc agcgaaaaac tattgatact tcccaccatt ttttgagctg aactaaaagc
      421 agaaacatca aacggtataa ctaacaataa agtcatcatc gatgagaatg caatacttat
      481 agcaatcggc atccctaata caagcaacac aacaaataca aaaaataaca ctaatccggc
      541 ttctattgcc attttattcc tccattttta tattttttct tgaattgctg gcttgttgct
      601 taatgtatcg attagattga tcaggctata gataatgatg agtacccccg atacaggtac
      661 tgacagataa acatatccca taggaatact taaagaaggc gagatctgcc ccattgtcaa
      721 tgtgacagca ttccatccgc catacaccat gatcatcgca gcaaatacca gaaataaact
      781 ttgaacaaca acttctaaaa ttttactttt tttgccagtt aatttttcac ttaaaaccgt
      841 tacggctaaa cgtgcttttt gtcccacaac atacgcggaa gacaacatgg caaaccagac
      901 aagaccgaat cgaacaagct cttccgtgac cgtatttgga tttcctaaaa ccgttctgga
      961 aaatacttgc cataaaacaa cagcgaccat cccaacaagc aaaacagctc ccaggatctc
     1021 taataccttg tttaaaatca ttttcattac agctacgctc cttgctcttg gatcttttga
     1081 taaaggtctt tgtaggtagg atcgtttttg aatatatcat gaagtggggc aactgcctct
     1141 cgaaacggtg tattatctac atcattgaat tggactccat gttcttcttg tgctaaagct
     1201 ttttcttctt caattgcttt tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct
     1261 gcttcttcaa tggctttttc ctgttcatca gttagctgtt ctttgatggc atcattcatg
     1321 atgatgatat ctggcacacg tgtatgctca tcataggaat agtatttagc taccccgcca
     1381 tgtccagctg tatacaagac aaattcattg ttttctgatc cgttgatcaa attggattga
     1441 agagacgtgt acacttcatc acttcccata ggtacgggcg aaccgcctaa aagctctacc
     1501 attttgatag cggtttcgct ttgcatcaca cggatcttct ttcccttcaa atcttccggc
     1561 gtatggatcg gaccgtctac catataaaag ctgcgctgac cagaatcata ataggtcaat
     1621 ccggtaaatc ctaaattttt ggtcgattgg tagattggct ccattatttc ttcattatcc
     1681 atcaccttaa agaaatgttc ttcatcattg aatagataag gaacggaaaa aatcgaataa
     1741 tctttagaga aactttccaa cgctgaggca cttacttttg tgaaatctac tgctcctgac
     1801 tgtgtcaatt cgatcagttc acgttctcca cccagttgtc catctgggaa atactggatc
     1861 tgtacggaac catctgtttt ttcttttact aaacgcccaa attcattcat tgcatcaatt
     1921 actggctgtt gcttgcataa gcaaagcgca aggtaactat ctcatttacg tctgcatctg
     1981 cgacagttgc cacactagaa ttatttccgc agccgcttag acctagcaga cctaggctaa
     2041 gcgaacttgc tgcaaacagc agtgatgttt ttttcatatg ggtttccttt cttttaacaa
     2101 taagagacga gcaattctgt caaacaaagc accgttaacg actgtccata aggcattgct
     2161 gtgattccga tattccgata aaaatctaaa ctgtccccca ttcctgtccc gattgaaacg
     2221 ttttgaactt ctcctttttc atcgatttgt tcaagcaatc cttggatagc acgataagcg
     2281 acttctttat attcttgtgg taaataacgt ttatggaccg ctttcaaaat accgtaagca
     2341 aatcctgcag ttgcagaaga ttctacgtaa gaagaaggat catccaacaa cgtatgccat
     2401 aaaccgcttt cagactgata agtttttaat gcacgcactt gtgcttcaag tgtttcgatc
     2461 aataatttgc gcaatgcatc ttctttcgat aattctaaga tttcaatgat ctctggaata
     2521 gcaatcgtca accagcagtt tccacgagcc caaaatgctt cggcatagtt atgatttcct
     2581 tcaaacgtcc agccgtgata ccacaatcct gttcgtttat ccatcaagta tttgatatgg
     2641 atcataaact ggtaacgagc ttcttctaaa tattctgggc gattcaacag tttcccgatt
     2701 tttgccaaag gaagcactgt catcatcaat gtatcgtccc acaactgatt cttattttcc
     2761 ggtccatatg tggcatgctg taacccgtct tcatttgtcc gaggcatgtc attcatgacc
     2821 cattctgccc attgttctaa ataaggcaaa tagcgtgaat ctttcgtgtc ttcatacaag
     2881 taagccatag tcaatagcgg tgccattgta ttgacgtttt tgggcggagc gccttcttcc
     2941 atacgatcct caaaccattc attgaccact tgataagcgc gaggattttt tgtcatctgg
     3001 taatttttat aaataccata tagaccgaca ccttgtggcc aattccacac tgtccagctt
     3061 ttgtcatcta ctttcagtcc atcaaagtcc aacaaaaatt caccattatc gtctttgatc
     3121 gttgtcaggt tttcaacgag caggtcaatt ttttctttca ctaattcttt aggtacaaaa
     3181 atctcttctt taataatcat ctttacgcca cctttttaaa cgctttcatt ttatatcctt
     3241 aatatagcag taataccgtc cactataaat atcgcttttt gaacaaaaac tgtctaaaat
     3301 tgacctaaat gttaaataac aaaagtaact ttcagttact tggaaattaa aaaaagaact
     3361 tggagaaaca acgttttcca agttcttttt catctatttt cttatctttt ttctacgctt
     3421 tcttttgctt atccgttgta agcatcaatg atgatctgtt tcaattcaga gatcaatggt
     3481 tctttcggat tagctgtcgt acattggtct tcgtaagcta attcagccat acggtctacg
     3541 gttgtatcta gtgtttcttg tgtgactcct tgagctttta gactcatgtt gatgccgaca
     3601 gattttccta attcataaac agccgttgct aaagcttcga ctaattcctc tgttgtttcg
     3661 cctttcagac ctaagaattt tgcgatatct gcgtaatctg tatctgcacg gaaatagtca
     3721 tatttaggga acatcgcatg tttttgtgga tcttttgcat tatagcggat aatatgcggt
     3781 aaaagaatcg catttgtccg tccatgaggg atcccgtatt ccccgccgat tttatgtgcg
     3841 actgagtgac aaatacctag gaatgcgtta gcaaacgcca taccagccat tgtagaagcg
     3901 ttgtgcattt tttcacgtga ttccatatca ggtgttttta ctgatttttc taaatagtca
     3961 aagactaact tgattgcttg taggctcaat ccacgtgtat aatctgaagc catgactgaa
     4021 acataggatt cgattgcatg tgttaacaca tccatcccgg tatctgctgt gacagatgct
     4081 ggaactgaca tgacgaattg aggatcgacg attgcgacat ctggcgtcaa tgcataatct
     4141 gctaacggat atttcacatg tgtttcactg tcagtgatga ccgcaaatgg cgttacttca
     4201 gaaccagtcc ctgatgttgt cgggatacat acgaattgtg ttttttctgg tttgtcgatc
     4261 ttatatgtcc gtttacggat atccaagaat ttttgttttg caccaaagaa agatgtgtct
     4321 gggtgttcat agaacatcca cattccctta gctgcgtcca ttgcagaacc gcctccaaga
     4381 gcgattactg tatctggctt gaagtctgcc atcagtttcg ttcctgcgta tacggtattt
     4441 gtcgatgggt tcggttcaac atctgagaag acttcgatct tcacatcatt tttacgtcgt
     4501 gccaatactt cacgaactgt atcgcagtaa ccgaattgga ccataccagg gtcacaaacg
     4561 atcatgacac gttcaacgtt ttccattttc tctaagtaca atagagagtt cttttcaaag
     4621 aagatttttg gcggtaattt gaaccattgc atgttatttc tccgtttcgc tacagttttg
     4681 acattgatta agtttacagc agagacgtta cgagaaacag agttcttacc ataagaaccg
     4741 catcccaaag tcaatgacgg aatcatttcg ttatagatat caccgatgcc gccttctgca
     4801 gatggtgtat tcactaaaat acgacaagct ttcattcgaa gaccgaattt gacatgcaac
     4861 tcttcgtttt ctgaatggat aactgctgta tgtcctaatc ctccaagatt caacatacct
     4921 tcacacaaat caaacgcatg gtccgtgttg ttagctttga tcatggcaag aactggagaa
     4981 agtttttctc ttgatagcgg ataatctggg cctacgcctt ccaattcagc aatcagcatt
     5041 ttcgttcctt ttggtacact gattcctgcc aattttgcga tttccatcgc agaatgtcca
     5101 acgattgatg gattgaccgc atattttcct tcgttcatca ctgcatcttc taatttttgc
     5161 aattcatctg gttttacgat ataaacttgg tgtgcttgga attctgcttt tactgctgca
     5221 tagatttctt tatccacgat cacagcttgt tcagaagcac aaatcattcc attatcaaat
     5281 gttttcgaaa cgatcaaatc gttcactgca cgtttaattt ttgctgtttt ttcaatataa
     5341 gctggtacgt tacctggacc tacacctaaa gctggttttc ctgtagagta agcagacttg
     5401 accattccag ctccacctgt cgctaagacg atcgcgatac ctggatggtt cattagcata
     5461 gaagtcgctt cgatcgaagg atgttcgatc cattggatac aattttcagg agctccggca
     5521 gcgatagcag catcacgtac cactcgtgct gcttctgcag aagatttttg ggcacttgga
     5581 tggaaagcaa aaacgattgg atttcttgtt ttcaacgcga tcattgattt gaaaatcgtt
     5641 gtggaagtcg gattcgttgt tggagttact ccgcaaacca caccgactgg ttcagcaatt
     5701 tcaatcaacc ctttttggac atcttcattg atcacaccaa ctgttttatc atgtttgata
     5761 ttatgccaaa tatattcgga cgcatacata tttttgattg ctttatcttc gtaaacaccg
     5821 cggcctgttt cctctacagc catcttcgct aatggcatat gttgatccaa agctgccatt
     5881 gccatttcat gcacgatatg gtctactttt tcttgatcaa agctttccat ttctttcaat
     5941 gctgcatttg ctttttctgc caaatcatca atcattgctt gtacgtcaat tgtcttcgtt
     6001 ttttcttttt ctattacttt agccatcatt atcctcctaa tgggtcatgg ttatttgtta
     6061 aataattcac atgtatatca tacaatattt atttttctat gtaaacactt ttttgtgatt
     6121 tttttcacag ttatttttaa atataaaatt gctttattta caggtgtttg tatagaaaaa
     6181 tatataaagt tatttattat tatttatatg acacacccga ttattcaata ttgagcaata
     6241 tatcccatgc tcttatctct ttcttttttg aataaaaaat gaggtaggac atctttctgc
     6301 cctacctcgt ctttaaataa gctttgatac ttatttttct tccttctctt ctgtctcttc
     6361 cagcgtttct gttgtttcag tttttacttc aactgtctca gctggttctt ctgttgtttc
     6421 aacagtagtc gttgcatcat ttgttacgac agtccctggt ttgaccgttt tgattgctgc
     6481 acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca actgtccgtt tgtcattgtc
     6541 gatttctgaa acaactccat gtaatccgcc gatcgttacg actgaatcac ctggtttcat
     6601 gctatctaac aagttttgac gttcttgctg ttgtttcttt tgcgaacgtg acataaaaaa
     6661 ccacattgct aatagcgcga taaacatcac aagcattggt aatgttccca tttttttcac
     6721 ctcactaatt ctgtatctac aaaaaacact ttagcagaaa aatgctgata acactagttt
     6781 tttacttaat ttttaaaaga ttttccttta gaagcttttt gcgttttctt tattgaaccc
     6841 gtattcttca aaaaaggctt ctctaaattc aagcaaatta tcatccatga ttgcttgacg
     6901 tacttgtttc attagattca acaagaagta taaattgtgg taagaagtca aacgaatccc
     6961 aaatgtttca tcacatttga tcaaatggcg gatataggca cgtgtatagt ttttacatgt
     7021 ataacaatca catttctcat caattggacg gaaatcatgg gcatatttcg catttttcac
     7081 tactagacgg ccttgagaag tcatacatgt gccatttcga gcgattcgag taggcaagac
     7141 acagtcaaac atgtctactc cacggataac accatcgatc aatgaatcag cagcaccgac
     7201 acccatcaag taacgtggtt tgttttcggg gatcaacggt gtagtgaatt ctaatacacg
     7261 attcatttct gatttaggtt ccccgacaga taacccgccg attgagtaac cagggaaatc
     7321 catcccgact agatctttcg cgctttgacg acgaagatct tcaaaccctg ctccttggat
     7381 gattccaaat aatccttgac ggtctggatt agcatgagct ttcaatcctc gttctgccca
     7441 tcgagacgta cgttctactg attttttcac gtagtcgtaa ctttcatcga atggtggaca
     7501 ttcgtcaaaa ctcatcatga tatcagagcc caatttattt tggatgttga ttgctttttc
     7561 tggtgataaa aacatacgcg aaccgttcaa gtgatttttg aaatgcacgc cttcttcttc
     7621 gatattccgc atatcactta atgaaaaaac ttgaaagccg cctgaatctg ttaaaatcgg
     7681 ttgatcccag ttcataaatt tatgcagtcc gccagcttct tctactaagt cttcaccagg
     7741 gcgtagccat aaatgatatg tattacttaa aataacgcca gcacccattt ctttcaactc
     7801 ttctggggac attgttttta ctgtagctaa tgttcctact ggcatgaaca ttggtgtggg
     7861 gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg ccagtatgtt tttctttctt
     7921 gattaaacga tagcgaatgg caggttgcgt cattcatttc cctactttct atataaaata
     7981 tcttgacaac tcccaccatc ataatcgttt caagtgcttt ttgcaagttc cctcggcttc
     8041 cttctacttt atttaatatt ctttttttta tccttttttt atgaataaca aaaaaaagaa
     8101 aaacattatg acagaaatat tttttgatac atggtatatt ggtgttatta aagaaaagga
     8161 gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa gcaaaagaga gtctaaaagg
     8221 acgttgggga caagctgttt tactaaatct aatccctaca ttgattggta ttgctactat
     8281 tcttcttatt atgattccta ttatcttgtt tgccaccacc atatatgatg gatcagcgag
     8341 cgtctcagaa gcgacaactt caggaggcgg tagttcagga ggcggtggcg gaattatttc
     8401 cagcatcatt agcgctttgt tcatgagcgg tatttcttgg acttatcttg atattttacg
     8461 tggacaaaaa gatcagatcg aaccattcaa agatgcattt cgtggattca aaggtttgtt
     8521 tatcggcgga gtgatcagct tagctgtgct gatctcgatc tttacaacat tatggggttt
     8581 gctattagtt attcctggaa tcattaaaag ctattcttat tctcaaagct attttattta
     8641 ctacgatatc gtccaagaaa caggacaaac acctaaagta ctggatacga tcactgcaag
     8701 tcgaaaattg atggatggtt ataaaggaca acttttctgg ttagatcttt catttatcgg
     8761 ctggcatatc ttagcattag caactgctgg tattggttat ctatggctca gcccatacat
     8821 ttctgcaaca aaagcagcat tttatgaaca attgccaaaa gatatttaaa agagagtgtg
     8881 acttgtgacc gagggggtgg caggagaaca ccgtttatcg agaggcagta aaagaaacat
     8941 tcagcccatt agcaaaaagg aggtgtgaca acattcatgt cacacctcct ttttgtatag
     9001 ttccagtaaa aagaaaatca acttagaaac agttaatcat tcctcttctt ctgcttccgg
     9061 taatcgatgg aacttgatca gttcattcac cgcgattggt acgatagata aaatccctac
     9121 caagaaccag tgattcaaac tgatgtgtac taggccgaat agttcttgcg taaatggcag
     9181 caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc atttctacca aagctttatt
     9241 ggtcgctaaa ttgactttga agatcgattg gctgctgcgt acattgaaca catgcagaat
     9301 cgatgaataa gctaagatca agaatgcaac agtttgaccg acatgctgac ttgcgtctac
     9361 ccatgggctg atagtatcga cataactacc taaataataa ccaaacaagg ttacgatggt
     9421 gaagacccct gcatttatgg caattttttg caataaacct cgagcaaaga tcccttcatt
     9481 tttcggaatg ggtggctgat ccatgatgcc gtgttccgct ggctctcttc ctaaagcaaa
     9541 tcctggaagt ccatcagcaa ctacattgac aaacagtagt tgcacagctg taaatggtgc
     9601 tccccatcct aacagcatcg caatcaaaac aataaagatc tccgaaatat tacagcttag
     9661 caaaaagttg atagctttac gaatattctg ataaaccgaa cgtccttgac tgacagcgtc
     9721 tacgatcgtc gcaaagttgt catccgtcaa aatcatatct gcggcacttt gcgccacatc
     9781 tgttcctgtg atccccatgg cacacccaac gttacttgct ttcaacgctg gtgcatcatt
     9841 cacaccatcc ccagtcatcg ctacgactgc acccgtacgt tgccaagatt tgacgatccg
     9901 gatcttgtct tctggcgtta cccgagcata gacagagaga gctttgacac gcgcatctaa
     9961 ttcttcgtct gataattgat gcagttctga tcctgaaagt gcttctgacg gatcattcaa
    10021 gatacctaat tctttcgcta tggcactggc agtcaccaca tgatctccag tgatcatgac
    10081 tgttttgatg ccagcctttt tcgctcgctt gattgctcct ttactttctg gacgtggtgg
    10141 atcgatcatt ccgatcagac caatcaaacg taagtctttt tccagtgctt cagaagaaat
    10201 atcttgcggt tcttcgtcat aagtagcata accaacagca atcactctca gtgcccgttt
    10261 accaaagcga tcattaacga tcgccgcttg ttctacatcg cctgaactaa agcgcggagc
    10321 aagaacatcg aatgctcctt tggtgattga gatgtatttt tcgcccattt ggtgaactgt
    10381 tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt actcgtggat atttttcttc
    10441 taattctgcc tttgtatgat aattctcttc aacagccctt acgatggcta cttctgttgg
    10501 attacctttg ataacaaggt cgtcatcttc ttgctcgacc gtcacatctg tacatagtgc
    10561 agccattttc aatatttcca tcgcgctgtt cgtcattgaa tcttcgatat tcgttacttc
    10621 atcttctttg ctccataccc ggcggacgcg catttcattt tgtgtcagcg ttcctgtttt
    10681 gtctgagcag ataacattag cggttcctag cgtttcaact gctggcaact gtcggatgat
    10741 cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt gtcaacgtaa caataacagt
    10801 caaggtttcc ggtacagctg caacagccaa ggaaatcgaa gtcatgaaca tctcaaggag
    10861 tggttctcct tgcagttcac caatcacaaa tactaaagcc gcagccgcta aagcgatcat
    10921 actgattcgt ttgcctagct gattcaatcg gacctgtaaa ggtgtttttt gcatagtatt
    10981 ttcgttcaaa agatccgcga tcttacccat ttctgtagcc atgccaatgg cagtgacgat
    11041 cgctttccca cgacctgcag caaccgtaca tcctttaaat accatattaa gttggtcacc
    11101 taatgactca tcttcttcag caataaatgc tgcatctttt tcaactgctt cactttctcc
    11161 agtcaacgcc gattcttcta cacgtaattg tgatgcttcg atcaaacggg catcggcaga
    11221 aatagcatca ccggactcta aaacaagaat atcacccact accagttgct ctgactcaac
    11281 agtggtcact ttgccgtctc gtatgacagt tgccgtttgt ttattcatat cttgcaagga
    11341 ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc aataccgagt tgatcacaac
    11401 gatagcgatg atcagcagtc cttcaaataa atccccatgt tctgtagcaa atgcggtata
    11461 aaaggaaatc accgctgcta caagaaggat gatcgttgta aaatcggaca agcttcgaaa
    11521 gaattttttg atcatcgatt ctttcggcgc ctcatcgaat ttattcaatc cctgcttttt
    11581 tcgccgttca tccacttctt gtgaagacaa cccagtatct ggatctgctt ctgttacctt
    11641 ctttacttct tctattgtct gcttgtattc ttccatccta ttcctccttt aaaatcttcg
    11701 tacaaaaaaa ggtacttatt ttactataac atcctttttt tatttacttc aaataaaggg
    11761 cttatcgatt atttgttaag aaagcgtaaa atttaaagtg cttatatttg caaacgctac
    11821 caaaggatgc tattatatag atgtaaggaa ggaggatacc atcaaataga cagaagaatt
    11881 tctcgataga acagtgtaag caaacaaagt tgttagaagt acaggtttca cccagacatg
    11941 cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga tgtttggatc acgaagtatc
    12001 cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg taaacgtctg aaaattgaat
    12061 aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa acaaaatttt tgtttaacat
    12121 gaagaaattc aacaccgtgc tttgcatgta tgatggaaag ttacaatcat ttctgtttat
    12181 ttgaatcctt ccgaggtaca agaaagccga gcttatcgag agctcggctt tttctttgtc
    12241 ataaaatctt tgtaaagctt ttttgttatt caaaactagc ggcttttcat aaccttattt
    12301 cacgaacatc gcatctccaa aactaaagaa acgatagcgc tcttcaatcg catgctggta
    12361 agcagataaa gtcaattctc ttcctgcaaa ggcactaact aacatgacta aagtcgattt
    12421 tggcaaatgg aaattagttg agaaagcttc taccactttg aattcatagc caggcgtaat
    12481 gaaaatatca gtccatccgc tgtctgcttt gatttctcca ttaaatttcg ttccaatcgt
    12541 ttctaatgtt cgaattgaag ttgttccgac agccacgatt ttcccgccat ttttacgtac
    12601 ttggttcaat cgctcagccg cttcttccgt caaacggtag aattcactat gcatatggtg
    12661 ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct aaccctacat gtagtgtcag
    12721 atagactaat tcaacaccct ttgcttcgat ctcatcaagt aattctttcg taaaatgcaa
    12781 tccagctgtt ggcgctgcag cagaaccatt ttctttcgca tagacagttt gatagcgatc
    12841 tgggtcttcc agacgttctt tgatatatgg aggcaatggc atttctccca atgattcaag
    12901 aatctctaga aaaattcctt catactcaaa gtcaatgatc cgtccgccgt gttccaattc
    12961 ttctttcact actgcttgca atctgccatc accaaaagag attcttgtcc caactttggc
    13021 acgtttcgca ggtttgatca atgtctccca cgtatctcct tgtgtattcg ttagaagaag
    13081 gacttctaaa tgcgcaccag tatctggttt ttctccatat aaacgagcag gcagaactcg
    13141 tgtattattc ataactagcg catcaccagg ctgtaactct tcaatgattt catggaaatg
    13201 tttatcttcc atctctccag ttttccgatc caaaacaagt aaacgagaat gatcgcggtc
    13261 ttttaatggt gtttgtgcga ttaattcttc cggcaagtca aaatcaaaat cttctgttgt
    13321 taacatatcg tcactcactt tctatcatgt gcttcatcat tctatcatgt ttttcgttct
    13381 gttggcaaaa tcagcacttt ttcacttcta ttttttgttt tcctaaataa aaaaactacc
    13441 ctatttgtat tttttcattg ttttaggcat actagtaata gatagttctt cattgaaaat
    13501 agaaaaagag gtggcaccat gattgaattt caaaatgtgt caaaaattta taaaggtgga
    13561 aaagtcgctg tagaggacgt caatctttca tttgaaaagg gtgagttcat ttgttttatc
    13621 ggtacgagcg gaagcggaaa aacgacgacg atgcggatga tcaaccgaat gactgatcca
    13681 acacaaggga aaattttgat tgatggaaaa tccatccaag aaattgatcc agtggaattg
    13741 cgacgtcaga tcggctatgt tatccaaagt attggattga tgcctcacat gacgatccgt
    13801 gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc aagaagaacg gaataaaaca
    13861 gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga tgttagatcg ctacccacat
    13921 gaattatctg gtggtcagca gcagcgtatt ggagtcgttc gtgcccttgc agcgaatcaa
    13981 gatatcatct tgatggacga gccattcggt gcgcttgatc caatcactcg tgattccttg
    14041 caagaccttg tcaaagattt acaagagcgt ttgggaaaaa cgatcgtatt tgtaacccat
    14101 gacatggatg aagcg
//
LOCUS       DACEZN010000061        13546 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEZN010000061 DACEZN010000000
VERSION     DACEZN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.061"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            96..1106
                     /locus_tag="IXH60_001452"
     CDS             96..1106
                     /locus_tag="IXH60_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1990385.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1221..1934)
                     /locus_tag="IXH60_001453"
     CDS             complement(1221..1934)
                     /locus_tag="IXH60_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990386.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2124..2714)
                     /locus_tag="IXH60_001454"
     CDS             complement(2124..2714)
                     /locus_tag="IXH60_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1990387.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3198..3425
                     /locus_tag="IXH60_001455"
     CDS             3198..3425
                     /locus_tag="IXH60_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1990388.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3441..4607
                     /locus_tag="IXH60_001456"
     CDS             3441..4607
                     /locus_tag="IXH60_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1990389.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            4841..5884
                     /gene="hrcA"
                     /locus_tag="IXH60_001457"
     CDS             4841..5884
                     /gene="hrcA"
                     /locus_tag="IXH60_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1990390.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            5978..6541
                     /gene="grpE"
                     /locus_tag="IXH60_001458"
     CDS             5978..6541
                     /gene="grpE"
                     /locus_tag="IXH60_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1990391.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            6592..8421
                     /gene="dnaK"
                     /locus_tag="IXH60_001459"
     CDS             6592..8421
                     /gene="dnaK"
                     /locus_tag="IXH60_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1990392.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            8572..9738
                     /gene="dnaJ"
                     /locus_tag="IXH60_001460"
     CDS             8572..9738
                     /gene="dnaJ"
                     /locus_tag="IXH60_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1990393.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(9820..10485)
                     /locus_tag="IXH60_001461"
     CDS             complement(9820..10485)
                     /locus_tag="IXH60_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1990394.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            10461..10634
                     /locus_tag="IXH60_001462"
     CDS             10461..10634
                     /locus_tag="IXH60_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1990395.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(10679..11416)
                     /locus_tag="IXH60_001463"
     CDS             complement(10679..11416)
                     /locus_tag="IXH60_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990396.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(11418..12920)
                     /locus_tag="IXH60_001464"
     CDS             complement(11418..12920)
                     /locus_tag="IXH60_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1990397.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13179..>13546
                     /locus_tag="IXH60_001465"
     CDS             13179..>13546
                     /locus_tag="IXH60_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1990398.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVND"
BASE COUNT         4446 a         2390 c         2822 g         3888 t
ORIGIN      
        1 tcatccagct acttgagatg ccgatatttt tgatgccggc aaacgaaaaa aacaattaac
       61 caagtaaaat agtgcatact tggaggtgaa gcaacatgat aagttatcca ttattagaaa
      121 gaaatgcaac gattggggtt actgcaccat cctcaggcgt atcagaggaa caccattcgt
      181 tactcgaatc agctttaaag agaatggaag aaaaatcttt ccaagtagtt actggaaaga
      241 cgctttggac gcaagataag gaaaaatcag gtccagcaag aaaacgagca gatgaattca
      301 atcaaatgat gcaagatgaa aatatccaaa tgatctttcc gacttgggga ggagaattat
      361 tgattgagat tctcgatttt gtcgattttg atgctctaca accaaaatgg atagtaggtt
      421 attcagatat tagtacgttg ctacttgctg tcacattaaa aacagggata gccactgcac
      481 atggaactaa ccttattgat ttaagaggag agtacttgga tgaaacgaca gcaatgtggg
      541 aaagtgtact gaaaacaaga gaaggagaat cgattctaca gcaatcttct gaaaagtatc
      601 aaaaagtgtg gccccatgag cattcatcat cttctgtttt ccatctcact gaaccaacaa
      661 attggcaatc agtcagcgga gatgcagtaa ggatgaaggg acgattatta ggtggttgta
      721 ttgatacgat caggcattta atcggtactc cttatggaga tgtccaatct tttaaaagaa
      781 agtataatga tggaaagccg atcttgtggt ttctagaaaa ctgtgagcta tcgtcagttg
      841 atttaagaag atcacttgtt cagatgaaac tggcaggctg gtttgatggc agctcgggga
      901 tcatgttcgg tagaagtgcg gcagaggatt tgttagatta taaaatgaag gatgtatacg
      961 aagatctgtc aaaggaatta aaactaccga tcttgtatga tattgattgt ggtcatgtac
     1021 cgccacaact gacattgatt aatggtgcct atgcagaagt agcggtagaa gatagaaaag
     1081 cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg ccttggagtc tatcaaagac
     1141 caataaatct aaatagaaaa gaaagtgaaa gaagctgaca aaatgctgtc agcttctttt
     1201 tgtataacat attagaaagt ctaattatct ataactgtca catcgattaa ctgtttcacg
     1261 tcattgggat ttcctgtagg aatagctact acagatgcat tatcaatgat ttcttgagtt
     1321 gtttctctaa tgaacgctgg ataagatgtt tgatctgcag gcatttgcca atgtgaagga
     1381 actggataac tagtgattgt cccgtcatgg ttacttgaat acactaaaac tccctggtaa
     1441 ctgtatttcc cagtgagcat aaccacatct ttcggataga ctgcactccc tgcatcataa
     1501 ggggcgatag gtgttcctgc aggataatga ataacatgta attcgaaatc acccgatgta
     1561 tctctaaaag tattacctct aatagctagc caaacacgtg cgtactcaat ttgtagatca
     1621 gaatatcctg ctaacggatc tattgactga gaagactgtg aggaggttgt gtctattgtt
     1681 gaagaagaaa tatttgtctc aactgtacta ctagatgtag aagaagtatt gggctctgag
     1741 actttactgc ttgttgaaga tgtattcgtt tcagtagaag ctgatttctg agtacttgat
     1801 ttttcagctg aactggtttg tctgattgaa cttttgtctg tggtcgcaga tggatcacta
     1861 ttttctttac taggagacgt acatcctaca aggaataaag agaccaacaa taaaataaaa
     1921 cctaactttt tcatataaaa tacctcatta tttttttaat accagtatag cataatcctg
     1981 aatttttgaa tccgtaaaac cttgtctatg aatataaata gagtgtatct tctttgataa
     2041 atgtcagaaa aagtgtgaca aataatcatc agtaaaatta cttgtcacac tattgataag
     2101 gagtatttaa ttgaagagaa tgattaggag aaaggatctc cgtacttttt atagtaatca
     2161 tagaatccaa gtttgtatag acgaatcgtt ttttctctta tttcttctgt ttcaaaatgg
     2221 actgcttgaa gaatcaagtc tgtgaattct ataggagctg ttccattcgc agtaatcaaa
     2281 ttacgatctt tcacagcttg ttgttctata aaatcttctt tattctgata attttccgat
     2341 tctaaccaag gaaattgcgc atttccggtg tgtttgtaac cttgcaataa tccgctctga
     2401 gctaaataat caactgcccc acaaatagca gctactggta cgtctgtttt taacaattta
     2461 tcgattattt gatataaatg atgatttttt atgtcccatg aattgccacc gatcataata
     2521 aaaaggtcaa ctttttcagg tatttgttcg attaagtaat cgatttttgt ttttaatcct
     2581 ccaatggaag tgacctctga ttggatagaa gcagttttgt tttgccattt ctcattttgg
     2641 tttagtgtgc tagtaatata agcaccttcc caatcggcat attcctctaa tagaaacgta
     2701 atcgcagttt tcattttcaa gctctccttt actatctata acaaggacaa cagttttaac
     2761 ctacagacat tcattaaaat tgggtagcga cagcgaaaac aacaattctt aagttttaga
     2821 cagagtatac agatagtcaa cttaattaaa tacgaactaa ataaaaatga caggtaatca
     2881 tttttgttta ccagcattat aacattttgc atacatggat gacaaaagaa ccaattccca
     2941 cttgcttgag tacaatatta aaagaaactg gaaggcaaaa agtaattata gaaacgaaaa
     3001 ataaatcata tcgggcactc agacatgaaa acaaccctag agatatataa ctaggtaacc
     3061 agtttaatga aagaaaaaat catccaagaa gtcgattctt ggatttttta gtggatttgt
     3121 tcgccaaata cgtttagtat gcgtatatga gaggttgtaa gcaaagcgtt gtggtctgag
     3181 cacttaagga ggaatctgtg aaaatagctt ttcatatttt tgcagatttt gacttaatgc
     3241 cgaaggtcag ctttgcgatt accgtttatc gagaggctgt aaaagcaccg tgtagctccg
     3301 atgacaataa gcaagcattt ttagagctag gtttccttga ttctaaatat gcggcttatt
     3361 tcaatgagtt ggtgcttgaa cgcgatttta agaaggtcga aaacaggcac gacttttcga
     3421 cgtaaggaga aagaatttca atgatagaaa caataaatcc cgcagaaaac aaggtttctg
     3481 cgtacattca tatcccattt tgcgagcata tctgctatta ctgtgatttt aataaagttt
     3541 ttttagaagg gcagccagta gatgagtatg tggaaatgct gctgaatgaa atgaaaataa
     3601 tgctagaaag tcatccgaca gcgaatttag aaacacttta cgtagggggc gggacaccaa
     3661 cttctttatc tgctaaacaa cttgatcgac tgttatcagg tgctagagag atcctgcctt
     3721 ttcaaaatgg taatgagttt actgtagaag cgaatccagg tgatttgacc cgtgaaaaac
     3781 ttcaagtaat gaaaaactat ggcgtcaatc gtttatccat gggagtgcag acatttgata
     3841 atcgcttatt gaaaaaaatt ggacgtaaac acaccgcaga agatgtgtat caaactatgc
     3901 aatttttaga agcagaaaat tttaccaatg taagcatcga tttgatttat gcattaccgg
     3961 gacaaacact agaaggatat ctggatactt tggaaaaagc tttggctttg gatctgcctc
     4021 actattcttt gtactcattg atcctcgaaa acaaaacaat gttcatgaac tgggtacgac
     4081 aaggaagact cgaactgcct gatcaggaaa cggaaaccag aatgtttgaa gagacgattg
     4141 aagcaatgga aaagcatgga cgtcaccaat atgaaataag taactttggg ctctccggac
     4201 atgaatctca acataacctc atgtactgga acaatgacca ctattttggg tttggagctg
     4261 gtgctagcgg ttatcttgga gatattcgtt accgcaacaa aggaccaatc caacactatt
     4321 tacgcccatt acgagcaaat gaacgcccgg tattggaaga agaaaaattg acacgaaaaa
     4381 atcaaataga agaagaaatg ttcttaggac taagaaaaaa aacagggatt actaaaaaac
     4441 atttctatga tcgctatcaa aaaacaattg aatcggtgta cggtgatgtg ctaagggaat
     4501 tagagcaaga agggttactt gttaatgaag aagaccgtgt ttatttgaca ccaagaggga
     4561 cctttttggg gaacaatgtg tttgagcgct ttttgtttga aaaatagttt aaagaatatt
     4621 tgttttttat ctgaatttaa ataaaaaaag ttcaaaaata caataaagga ctattgaaac
     4681 gttgatataa ctggatttac atcaattatt tagaattagc actcttgtgt ttagagtgct
     4741 aaaaaatgga gtctttctct tgacaggaga agggcttctt gctatattta taagtggatt
     4801 agcacttaga atatatgagt gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa
     4861 cgacatttta cgtctgatca tccaacacta tacgagtagt ggcgtgcccg ttggttccaa
     4921 aacattgatg tctgagggag tcaaggccag ctcagcaacg attcgcaatg atatgaaagc
     4981 attagaagat gaaggattgc tgttgaagac tcactcttct tctgggcgga ttccatctgc
     5041 tttaggctat cgttattacg tggatcattt gttaaaacct gctcgcgtag cagaagatga
     5101 tttgctgctg attcgtcagt ctctaggcag agaatttcat gaaatcaatg agattattaa
     5161 gcaatctgct gagatattat ctgagctgac aagctatacg actttttcat tgggacctga
     5221 aataaaagac cgccgactga ctggctttcg tatcgtaccc ttgaataatc gacagattat
     5281 tgctatcgtc gtaacagata tggggaatgt agaaagccaa gtgttcactc ttcccgaata
     5341 tatgggaagt caggaactag aaaaaatggt acagattgta aacgaccgtc tagtcggaga
     5401 tcctctgatg acggtgtacc ataaacttcg tacggagatt ccgatgattc ttcataaata
     5461 tttccaaacg acagaaggga tgtctcagtt atttgacacc gttcttggtc atgtttttga
     5521 agataaggtt tatgtcagtg gacaaatgaa tctcttggat tatgagcccc gtcaagatct
     5581 tgatcagttc aaatcgatgt tttcttttat gaaagactca gacggactta cacaaatgat
     5641 cactccgatg gatagcaata ttcatatcaa gattggtgct gaattaggca atgatttatt
     5701 gcagaatatg agtatgattc aagcaagtta cgagatagat ggtcatggaa gaggtacaat
     5761 agccttgtta gggcctacta gtatgcctta ttctcggatg ttcggtctag tcgatgttta
     5821 tcgaagagaa ctagcagcta aacttgctga ttactaccgt tcgcttgact tgtcaaactc
     5881 gtaatgtgaa taacgaagct atttgcagct gatcgttctc tctttcttag taagagcaat
     5941 caaacagctg actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact
     6001 agataaagaa atgacagatg ctcagccaga accggaaatc gatgtcgaag ctgcagaaga
     6061 ttcgggtatt tcagaagctg aagcggaaga attcgaaaca gcgaaattaa aagctgagtt
     6121 agaagaaatg gaagataaat acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg
     6181 aggaaaaaat gagcgtgagc aattacaaaa ataccgttca caagatttag ccaaaaaatt
     6241 acttccatca attgacaacc tggaacgtgc attagccaca gaagtgtcag atgatcaagg
     6301 ggcaggtttg aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg cactggaaga
     6361 agaaggaatt gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc ttcatcaagc
     6421 agtacaaact gttccagcga ctgaagatac accagccgat acgattgtag aagtactaca
     6481 agaaggctac aaattacatg atcgcgtgtt aagaccaacc atggtcattg ttgcacaata
     6541 attgtgcgga tataaaactt acactttcaa ttaaagaagg agacgttaat tatgagtaaa
     6601 attatcggta ttgacttagg tacgactaac tctgcagtag cagtattgga aggaaacgaa
     6661 gcaaaaatca tcgctaaccc agaaggaaac cgtacaacac cttctgttgt atcattcaaa
     6721 aatggagaaa tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg
     6781 atttcatcaa tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa
     6841 tcatatacac cacaagaaat ctcagcaatg atccttcaat atatcaaagg attcgcagaa
     6901 gactatttag gtgaaaaagt cgaaaaagca gttatcactg tgccagctta ctttaatgat
     6961 gcacaacgtc aagcaacaaa agatgctggt aagatcgctg gtctagaagt agaacgtatc
     7021 gtcaatgaac caactgcagc tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa
     7081 aaaatcttag tattcgacct tggtggcggt acttttgacg tatctatcct tgaactagga
     7141 gacggtgtct ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg ggacgacttt
     7201 gataacaaaa tcatcgacta catggtagca gaattcaaaa aagaaaacag cattgacttg
     7261 tctcaagaca aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat
     7321 ttatctggcg tatcaagcac acaaatcagc ttgccattta tcacagcagg agaatctgga
     7381 ccattgcatt tggaaatgac attgacacgt gcaaaatttg atgaattaac agctgactta
     7441 gtagaacgta caaaagtacc agtacgtcaa gcgctaaaag atgcaggttt atcacaatca
     7501 gaaatcgacg aagttatttt agtcggtgga tctactcgta tccctgcagt tgtagaagca
     7561 gtacgtaaag aaacaggaaa agaaccaaac aaatcagtta accctgatga agtagttgct
     7621 atgggtgctg ctatccaagg tggcgtgatc actggtgatg tgaaagatgt tgttttactt
     7681 gacgttacac cattgtcatt aggtatcgaa acaatgggtg gagtgttcac taaattgatt
     7741 gaccgcaata caacgattcc aacaagtaaa tcacaagtat tctcaactgc ggcagataat
     7801 caaccagcag tagacatcca tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa
     7861 actctaggaa gattccaatt gacagatatt ccagcagctc ctcgtggtat tccacaaatc
     7921 gaagtaacat tcgatattga taaaaacggg attgtaaatg tatctgcgaa agatctaggt
     7981 acacaaaaag aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc
     8041 gaacgtatgg taaaagatgc agaagcaaat gcagaagcag ataaagctcg taaagaggaa
     8101 gttgacctac gtaatgatgt agatgcattg ctattctctg tggacaaaac attgaaagaa
     8161 ttagaaggca aagtggacga agaagaagtg aaaaaagcag aaacagctcg cgatgaattg
     8221 aaagccgctg ttgaagcaaa caacatcgaa gaaatgaaaa ctaaacgcga tgcattgaac
     8281 gaaatcgttc aaaacttgac tgttaaacta tacgaacaag cggcacaaca acaagctcaa
     8341 gaaaacccag aagcagctca aggcggagca gatgatgtcg ttgatgctga ttttgaagaa
     8401 gtcgatggcg acgataaata atcagttcac tgaatttcga gagaaggcca aagttgctac
     8461 tttggctttt tcttttgaaa aatagtttga agtatcggtt tttgtttcaa acaaaatatg
     8521 atatgataac aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc tatggcaaca
     8581 aaacgtgatt attatgaagt cttagggctc tcaaaaggag catcagaaga tgaaataaag
     8641 aaagcttatc gtaagctttc gaaaaaatac catcctgaca tcaataaaga acctgatgcg
     8701 gaagaaaaat tcaaagaagt atcagaagca tatgagattt taagtgaccc gcaaaaacgc
     8761 gctgcatatg accaatacgg gcatgcaggc acagatccaa attacggtgc tggtggagct
     8821 ggtggcggat ttggtggatt cggcggcttc tctggtggcg gcttcggtgg ttttgaagat
     8881 atcttcgact ctttctttgg aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa
     8941 ggtgctgatt tgcaatatac ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa
     9001 aaagaaatca agtataatcg ggaagaaatc tgtcatactt gtggtgggaa cggtgcaaaa
     9061 ccaggcacac aaccaactac ttgtcataaa tgtcatggtg caggtacgat taatgtagaa
     9121 cgccaaacac cacttggacg agtgatgagc cgtcaaactt gtgatgtttg tcatgggaca
     9181 gggaaagaaa tcaaagaacc ttgtccaacc tgtcatggct caggacatga gaagaaagca
     9241 cattcagtta aagtgaatgt accagctggt gtagaagaag gacaacaaat gcgtttagct
     9301 ggtcaaggag aagctggaga aaacggcgga ccatttggtg atttgtatgt ggtcttccgt
     9361 gtagaagaaa gtgatatctt tgatcgtgaa ggttctgaaa tttactatga attgccttta
     9421 agttttgtac aagcagcttt gggggatgaa gtccaagtac caactgtcca tggagatgtg
     9481 aaattgaaga tccctgctgg aacacagaca ggtacgaact tccgtttacg tggaaaaggc
     9541 gctccgaagt tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca
     9601 ccgaaaaatc taaatgaaca acaaagagaa gcattacgtg cattcgctga agctggcggc
     9661 atcaaagttg aagaacagca agaagacggt ttcttcgata agtttaaaga tgcttttggc
     9721 gggaaaaagc ggaagtagtt tttctagtaa aaacgctaag tcattaataa ttaaaaacca
     9781 gacgaacaat catatgttcg cctggttttt ttgtagttgt catgtttgta ccaacaaaaa
     9841 agccagcttt agaatatgat aatcgtcata gtttctaggg gtataaccag tgagagaatg
     9901 gaacttgttc aatcgatatt gcaaggtgtt tttatgaata aataattctt ctgagcaatt
     9961 tttgatgctt ccgttatttt tactgaagca tgccaaagtc ttcttgaaca aatgtacttc
    10021 ttcttctgtc aggcttttca aaatacgttt tacatataat gttcgttgat ctgacgggat
    10081 tgctggtaaa acgatcccga ggtctaaatc ttcaaaaaag cggatctcgt tcacagattg
    10141 atggaaagct atccattcgc aaacttgttt ggcatgaagg aaagattgcg gtgtctcttg
    10201 ctctgtataa caaagcgacc caattccaaa gtttagctgt aactgataca ttttattaaa
    10261 ctttgtctgg attttatcaa gcaatgatag caatgaggct ctggaaatcc gattgactag
    10321 gataatgatt tgttgtctat gtacagtgag caaagcctgt ccgtaataag agaagaccct
    10381 ttcgagcaac tccaataaag gattgatccg ttcttgacac aattgctgat tgtcagaaag
    10441 ttgtcccaca actacataat atggagaaga aaaatcaagc tcaattaagt gagggtggta
    10501 tcgactatgt acttgatatc atcaatgaac cgatggtact ttcttatgcg atggaacagg
    10561 tagagcgatt gcttgtttgt gcaggaaaac aagcgatgcg gctgatacga ttaggtgaga
    10621 aaataagaag ataaaaatac acggtccctt catggcgacc gtgcattttt ggtttttatt
    10681 aaatatttaa tactttattc aagaaatctt ttgtccgttc attttgcgga ttttcaaaga
    10741 tttgttgagg cgttccatct tctaaaaagt ttccgtcgtc aatgaacatg acgcgattgg
    10801 ccacttcttt agcgaacccc atttcgtgcg taacgataac catcgtcata ccttgttctg
    10861 ctaacttttt catgacgttc aagacatcac cgaccatttc aggatcgagt gcagaagtag
    10921 gctcatcaaa aagcatgatg tcagggttca tcgctagagc acgagcaata gccacacgct
    10981 gtttttgtcc accagaaagc atttcaggat acatatcttt tttatcttct agaccaactg
    11041 tctgcagtaa tttgattgct tttttctctg cttcttctct agattctttc ttcaggtcca
    11101 tcggtgctaa aatgatgttt tccaaaatag aaagatgagg gaataggtta aagtgttgga
    11161 ataccattcc gatatgttgt ctcaccaagt tgatatctgt gcttttatcc atcaagtggg
    11221 cgccgtcaat aataatttca ccgtcagaag gttcttctaa acgattcaag caacgcaaga
    11281 atgtactttt tcctgaacct gaagggccaa tgatacagac gacatctcct tcgttgatgg
    11341 atacattgat gtcgtttaat actgtgttat cgccgtattt tttgactaaa tgctcaacta
    11401 agattttttc tgccatctta cttcaccttc ttttctaaaa cttttgctaa ttttgtcaat
    11461 gctgtgatca aaatcaagta catgatcgca atgatcaagt aaacatacgt actttgtgtt
    11521 gtacgtgcaa caatgatctt accagtttgc agcaattcga ctacaccgat tgcggaaatg
    11581 atcgttgtgt cttttaatga gataacgaac tgattgacga atgatgggat catgatgcgt
    11641 atcgcttgcg gcaaaatgat tttttgcatt gtccgattat aagaaagacc taaacttctg
    11701 gaagcttcca tttgtccgac aggaacggcg ttgatcccgc ctcgaacaat ctctgcgata
    11761 taagcacttg cgttaagagt caaggtgata acaccagcaa ggaagtcagg aatcgtaaat
    11821 ccaacgatac caggcaaccc aaagaaaata aagaacgcaa gaaccatcat tggaattcct
    11881 cggatgatat cgacataaat actagcaatt gtgcgaagag cgcgtattgg tgctacactg
    11941 aataatccaa aaatcacacc gattactata gccaaagcaa aggagatcaa agttaggaga
    12001 attgttctcc aaagtccttt caacaatgct tgatagttgt tttggataag tccaagaaat
    12061 gttgattcat cagcagtgga agactcactg gaagtttctg aaccatccgc tacatattct
    12121 ttaataatct tatcgtattc gcctgttctt tttaattctt tcaatccttc attgaacata
    12181 ttaagaagct caggattttg tcctttttta acggcaaatg catattttcc gccagattct
    12241 tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg cataaccgat taccggataa
    12301 tcatccatca ttgcatcgat tgcgccaact tttaacccgt cgtacaattg atcagcagca
    12361 tcaaaatatt tgatagaata gccatattca gattcatggt cgcttaaaaa ttcagcactt
    12421 tcggtaccaa cttttgctcc gactgttttt cctttcaaat cttcataaga cttgatcgaa
    12481 gtatctcctt ttttgacagc taattggatc ccgctatcaa agtaagaatc agaaaaatca
    12541 tagcttttct ttcgttcatc tgttacagtc attccggcaa tcattccgtc tgcttgtcct
    12601 gattccaaag cttgcaaagc agcagagaaa ccacggtagt ccattcggat gttaaatcct
    12661 tgcagtttgg cgatgttttc catcaatttt acatcgatgc cttcgtactc gccctttgcg
    12721 ttttgaaatt caaatggagc aaaagcagag tcactggcaa ttacgtatac gtcttttttg
    12781 ggctctattt tttgcattgt ctcgcttgtg cttgactctg ccgttgtagt gttgtcagct
    12841 tgtgcaacat gaccaaacgc aagtgtcaca agtcctgtca ttaagaaaac aaatgagtga
    12901 aataaatgct ttcttttcat aatcaacctc ctaaatttcc aatattttat tattataggc
    12961 gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa tgaaaaaaat gcgagtttct
    13021 aacattttat gttataaaac ataacatgta taataagata aaaaagtggc gaaagtatgt
    13081 tcggtgtggt ataatttttt agtaaggaat gcctttgctt tccttttctt tttattggtc
    13141 gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat gattgagaga gatacctccc
    13201 gacactttga gcttgtctcg aaataccaac cggctggtga tcagcctgaa gcgattaacc
    13261 aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat cttattaggt gcgactggaa
    13321 cagggaaaac atatacgatt tccaatctta ttgagaaagt gaataaacca acactgatta
    13381 ttgcccataa caaaacattg gctggtcagc tgtacgggga attcaaggaa tttttcccta
    13441 acaatgctgt tgaatatttt gtttcttatt atgattatta tcagcctgaa gcttatgtgc
    13501 cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa tgatga
//
LOCUS       DACEZN010000062        13380 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEZN010000062 DACEZN010000000
VERSION     DACEZN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.062"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(170..859)
                     /locus_tag="IXH60_001466"
     CDS             complement(170..859)
                     /locus_tag="IXH60_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1990399.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(922..1686)
                     /locus_tag="IXH60_001467"
     CDS             complement(922..1686)
                     /locus_tag="IXH60_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1990400.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(1714..2412)
                     /locus_tag="IXH60_001468"
     CDS             complement(1714..2412)
                     /locus_tag="IXH60_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1990401.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(2424..3203)
                     /locus_tag="IXH60_001469"
     CDS             complement(2424..3203)
                     /locus_tag="IXH60_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1990402.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3219..4163)
                     /locus_tag="IXH60_001470"
     CDS             complement(3219..4163)
                     /locus_tag="IXH60_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1990403.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4209..4988)
                     /locus_tag="IXH60_001471"
     CDS             complement(4209..4988)
                     /locus_tag="IXH60_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990404.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
     gene            complement(5373..7448)
                     /locus_tag="IXH60_001472"
     CDS             complement(5373..7448)
                     /locus_tag="IXH60_001472"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1990405.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(7450..8367)
                     /gene="glyQ"
                     /locus_tag="IXH60_001473"
     CDS             complement(7450..8367)
                     /gene="glyQ"
                     /locus_tag="IXH60_001473"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1990406.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(8753..9565)
                     /gene="recO"
                     /locus_tag="IXH60_001474"
     CDS             complement(8753..9565)
                     /gene="recO"
                     /locus_tag="IXH60_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1990407.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(9708..10607)
                     /locus_tag="IXH60_001475"
     CDS             complement(9708..10607)
                     /locus_tag="IXH60_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1990408.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(10622..11023)
                     /locus_tag="IXH60_001476"
     CDS             complement(10622..11023)
                     /locus_tag="IXH60_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1990409.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(11001..11477)
                     /gene="ybeY"
                     /locus_tag="IXH60_001477"
     CDS             complement(11001..11477)
                     /gene="ybeY"
                     /locus_tag="IXH60_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1990410.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(11506..>13380)
                     /locus_tag="IXH60_001478"
     CDS             complement(11506..>13380)
                     /locus_tag="IXH60_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1990411.1"
                     /translation="DTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENV
                     AFYQKLPNNFYSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQ
                     YLNVTTEQQQMIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGN
                     QIDASAMKKLELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLF
                     YVTAMSVSVVLMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVS
                     ALFIFYDSIGTNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVIL
                     IIYQGMSFGDATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNH
                     PLLKQLLEEAPGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVE
                     NLPSGAENPHNFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYF
                     YVKAKERNPEVTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHN
                     LIEERISDGQLDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE
                     "
BASE COUNT         3566 a         2847 c         2378 g         4589 t
ORIGIN      
        1 atattttctt ttatataaga ataatgatac tgaattgccc cattcacctt tcttttttta
       61 gattttctac aagtgattca aaccctccat aattacagtt attacttttg aaacgataat
      121 atgaacatgt gtaatctttt ttattaacaa tccatcattc tatccctaac taatatgctc
      181 cattgggctt gacaataaca taaaacgttt ttaaaatcaa cctcaagtcg tacattaaac
      241 ttcgatgacc gatatactct aaatccaact caaccatttt atggaaacta acattatttc
      301 tatcactaac ttgccacaaa cctgaacaac caggttttac atacaagcgt tgaagatcat
      361 atctcgtgta ctcagctact tccctctcca aaggtggccg tggaccaatt agactcatat
      421 cgccttttaa aacattccat agctgtggca attcatcaat gcttttggca cggataattt
      481 tacctatttt agtgacacgc ggatcttctt tcatcttgaa cattgcacct tctatttcat
      541 tttgatcaag taattcttcc aatttttctt cagcatctaa acacatcgaa cggaatttat
      601 gcattttgaa tgtttttcca tttttaccaa cacgtgtatg gctaaaaaat attggacttc
      661 ctggctcttc ttttctgatt cgataagcta catagaacat caaaggagaa gctaaaatca
      721 aaccaataat gcttcctaca atatcaatca ttcttttaaa aaataaatag atatatcgtt
      781 tttcaacttt ttctttatcc aaccaaactt gattaggatg tacttcacta atacgagata
      841 ataaatcttc actttccata ctttcagtcc ctttcactat cttcactctc tcctcatttg
      901 tttttatcta cgaataaaga actaaaataa accaaacttc ttctttttta cctcttcata
      961 gattggataa ctgatctcat ccccgttgat cagatccttt gcgacttgtt tcatctgtac
     1021 tactttttcc ttaccaaagt ccttgacaat ctgctcatag cactctttta agtaaaaggt
     1081 gcgcttagag accccgtgtg catcagaagc agccatctgc acgagattat gtttgaccat
     1141 tgttttggct gttttctgga tgtctttgcc aaatacacca acataacttg gcgcagtaat
     1201 ttgggccaaa catcccatat ctaggaacgg aagcaaatga tttgggtctt ctcgaaaatg
     1261 cgcgttgcgt tctggatgga cgatcactgg tgtctttcct agttctaaca actcaaaaaa
     1321 aagccattct gcatataacg gcacttccag tgtcggaaat tcgatcagaa gatatgtatc
     1381 atctaaatct gtaaataaaa tttcatctcg gtggatgtct tcgatgagcg ttccggtaat
     1441 acgtacttct tgtccttcca ataacgtcaa aggcaactgt cttttctcta attcggcttg
     1501 taacgaagcg actagagaaa tgacttgtga tttttcattg ctgtattttc cattattgtg
     1561 gtgtggtgtg cataaaatat gcgtgatgcc ttgctgaatc gctttttcag ccattgcaat
     1621 acttgcttct aagttttctg ccccgtcatc gattcctggt aggatatggc aatgtagatc
     1681 gatcatttct cttgtctcct ctcttcttat tccttaattt ctgtagtaat aaccagcatc
     1741 tttgctatgt tctgctccgt tgtatacaac acctaagact cgtgcttgta ccatctctag
     1801 cagttctttt gctttagtca aggattcttt acgtgatgta ttttcacgta cgaccaataa
     1861 agtgccatcc gtttttgaag acatgatctg tgcatctgtt acggctacta ctggtggcat
     1921 gtcgaagatc actacatcaa atagttgtct tgcttctgct aaaatctgat ccattcttgg
     1981 cgagcttaat aactctgatg gatttggtgg tttaggtcca cttggcagaa tcgacaaatt
     2041 ctcgactggt gttcgttgga tctcatctgc tacacttcct gatgaactaa gtgctgtact
     2101 taatccactg gcattgttca gctggaatgt ctcatagacg acgggtttac gtaaatctgc
     2161 atcgactagt agaactcgtt gaccggattt agcaaaaacg actgcaatgt ttgctgcaat
     2221 ggtggatttt ccttctccag gacctgatga agtcacgaca atcgttttga tttgctgacc
     2281 agctgcggag gcaaactgga tattcgtccg gatcgtacga tattgttcgg cgattggtga
     2341 agatgggttt gttaatgtga tcaaactgac tacatgagtt tgtgctgctt tttgtttctg
     2401 tgttcgtgcc atttttcttc ctcctagact cttgagcggc ttctgcgtga tggagctgtt
     2461 ttatcgtttg tagcttttgg tcgtgcagga gctgatcctt ttttacggat accagcgttc
     2521 aattctttcg ctgacatttc tggaactgtt cctaagattg ggaatcctaa tgtatccgtc
     2581 acatattttt catctttcac tgtccgatca agtaattcga ataagaatgc caaacctacg
     2641 cctaccatca ttcctaatac taagccaatc actagattca atttattatt tggcgaaaca
     2701 ggtgtcgtat tggcaactgc acctgagatg atcgagattt tatcgacatt catcacgtct
     2761 tttgcgttct cttggaaaac ttgtgaagtt gtattagcga tctgttgagc agtcactgca
     2821 ttcgtgctta ttgcctggat cgagaacatt tgtgagtttt ctgactgatt gacggagatc
     2881 gcatctttga tttgtccagc ggtcattttc acattgtctt ctgtttctag acgatctttg
     2941 acttcattta atactagatc actaacgatc atatctttat acgtattgat catttgcagg
     3001 ttcgtattga catcattgac attcgctgtg tctgattgcg gcagtgtcac gatcaattgt
     3061 gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta cgattcctga gatggccaag
     3121 ccagcaaaca ttgagatgat gattgttgcc aagtgtttgc gtaatgttcc aaaaatttct
     3181 ttcaagctga ttgtctcttc cattttttct ccactttcct atttattctc tagattcatt
     3241 tgatctttta attctttttt cacacgatca agttcttgat cgctgacttt ttgataggag
     3301 atattatctt gcatgaagcc ttctccttgc agctgatctt gcttgatcgt accaaatgcc
     3361 tcccgataat ctaaagcgat ttttttcatt tcatcaaagc tcatgtctgt tttcatattc
     3421 gattctagag cattcaaaac ggattgatag ttgctgacac cgtttagact caatactttt
     3481 ttcgctacac cttctacgat ctttctttga cgctcttgtc taccatagtc accatttgga
     3541 tcttcataac gcatacgaga ataagataat gcctgatccc cgttcaatgt gattttaccg
     3601 attgtaaagt catagccatc ttgtgagaaa gtgaggtcat tattgacttc gataccacca
     3661 actgcatcaa caagttcttt tagccctttc atattgatag agacatagtg atcgattggg
     3721 atgtctacat atttttgtac ggtatccatc gccatggcag ctccgccgaa tgcgtaagcg
     3781 tggttgatct tgtcttttgt gtcacgacct acgatgtcga catacgtatc tcgaggaatg
     3841 ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg caaccatcat cgtatctgat
     3901 cttccttgat cgacccgacc taaatctcca gtatcaatcc ccatcaatag aacagagaaa
     3961 gattcttgtt tgtccagatc aactggtttc gttcgttctc cttgggttcg ttccactgat
     4021 tcgtaagttt tctcaataga atgtgagaga tccagatata attttgctcc tactcctaca
     4081 attgctaata ctaatacgat aagaactgct aaaataatca gaaatatttt tttccctctt
     4141 gattttcttc ttcgtcttgc catagtttcc tccgtttgct aaccttgctt gaatcatcgc
     4201 tttaactgtc aatagtttat attgctatag cccatttgct gagcaacagt ttgtgctttt
     4261 gttgcgtatg ctacgcttat tttttcattc cggatctgct ctatttgact gagcgccgtc
     4321 aaatattgat cgtaagtagc ggtggcagtc acttgaccat cctttaacat cgtatcgaat
     4381 aactgatcta gttgcgtgac ttgcgcgatc tgggtatcag cgaatcctag atattgcata
     4441 acaaccgttt tccaaggact atctttaaaa aagctcatat tttcacgtac acgagcgaca
     4501 tctgcacttg tcaccctttc tttgatgatg acatcgtcta cagcttgctg ccagtttgat
     4561 acatttttag taaataaatc gttgacagct gtctggatct tcagttttga ttctgcacta
     4621 tctaactgtg tcacgattgc tttcttctca tcttggattg caacagcttc tttaggcaat
     4681 tctgattctt tgatgtcgta atcatctgct gaaagtttga cagaggatag cttatttgac
     4741 aaacttgtga ttgtttcttc tgttatttca gggtttagaa aaattttttc ttttgtacta
     4801 tacagcttgt ctacttcttt tgataaagaa gtcaaggtgt ctttttgatt ggtgatggca
     4861 gattctgcat aattgatccg ttgctgatgt tgccgctgat tattgatatg gtatccaaac
     4921 aaacagatcg caatagcacc tgcacctacc gccacagcag tcaatgactt gacgaatggc
     4981 ttttccatag attcgctcct aagtattgat gatagataaa ttaagatata attcacttac
     5041 ataaaaatga cagtttttta tttaaaaaaa cataattaaa ctaatatatt cacagaaata
     5101 tttgtaaaca agttttccct atataatcat accacaaact gctttcttga ctagggaaat
     5161 ttacgattta aaagtaaata aaacattaat aagataacaa ttaatcgatt ttacttttat
     5221 ttccttaatt ttcataggtg tttttaaaag ttacacaaaa aataaaataa caaaaaaaat
     5281 taacattgaa ttaataatag ttatttataa caaataaacc agagaaatag cctattttgt
     5341 gcctgttctc tggtttattt acatattaga aattattttg tgatgatttg atctaaactt
     5401 gctagtgaca aagccatggc agcaatttga cttaattgcg ttaatcggtt cttacgtact
     5461 gctggttctt ctgccatgac catcgtctga tcaaaataat tttcgatcag tgggcgaagg
     5521 cttacaagct gatcataatt ttcgttgatc gtttggtcag caaagcttgt tttcattgcc
     5581 tctactgctt tgtgcagttc tttctccgct tcgttttcga acagttcagg gtctacttct
     5641 gattgttttt caagctccgc ttttttcgct aagttcacga cacgtgtcaa agcttccatc
     5701 gatggtttga agtcttcatc tgcaagatgc tgcttgaaca attgtgctgc tgcaaatact
     5761 tttgtcagat ctttttgttc tgcattcaaa acagcttcga tgacatcatg acgaatattc
     5821 ttcgttgtca ataattggcg cagacggcct ttgataaagt cgatcacctg ttgttgtccc
     5881 gctgacaagc caatgccaaa acgatccgca tctttactga taacttcact aatcgtttct
     5941 tgcagaacag ataaaggaaa ggcccatttt ttcgattcga tgattcgaac gacaccatat
     6001 gcttgacggc gcaaagcata tggatcgttt gagccacttg ggatcatccc aactgcaaag
     6061 aaagaaaaga tactatctaa tttatcagca atcgctaaaa cagaacctac tgtagtctca
     6121 ggtagatctc cttcactgga tataggcatg taatgttcac ggattgctgt tgctacggct
     6181 ggtttctcgc ctttaagcag agcatatttt tctcccatga tcccttgtag ttcagggaat
     6241 tctcctacca tgttggttac tagatcaaat ttatagattt cagcagcgcg ttgcagatcc
     6301 atcaattctt cctcagatag tcctacttgc tcgcctatga tgcgggcgat gacttgtacc
     6361 cgttgcattt tctcgtagat gctaccgatt ttttcatggt aagtaacagt ttttaatttt
     6421 tcaacgtatt ggtcgattga agctttcttg tcttcattat aaaagaattc cgcatcttct
     6481 aaacgagcag tcaatacttt ttgattgcct cgaaccacat tttccaattt tacccgatca
     6541 ccattacgag cagaaataaa atgaggaagc agcatacctt gatcattccg tacctcgaaa
     6601 taacgttgat gttctttcat agaagtgact agtacttctt caggaacaga aagatatttt
     6661 tcttcgtagt cgccagaaaa gacagttgga tattcgacta aattgttgac ttcttctagt
     6721 aaatcttcgt ctagatcaat aacccagtga tttttttctg ctaattcgtt tgcttgtgta
     6781 acgatcattt cttgtctttt ttgcggagac gcaatgacga attgttcatt caatttttct
     6841 tcatattctt ttgcatgcgc aaatgtcaca tcttcgccta agaaacgatg acctcgagag
     6901 gctctgcctg ttttgacatc caagatttcg aatgggatta cttcatcatc taacaatgcc
     6961 acgatccagt gaatagggcg gatatattca aaatcgtatt ctgcccaatg catcgttaca
     7021 gggaaagtca ggcttttaat gacatctgct aaacctgtca atacttcttt ggattcctta
     7081 ccatgagtgt atttcgttac atagacatat tccacacctt ttaattctct aaaagtgata
     7141 gcgtctgtcg tcagaccttg tccgcggaca aatccttccg ctgctttcgt ccagtttcca
     7201 tctgcgtcca aagcaatttt tttggctggt cctttgactt cttcttcact gtccgcttgt
     7261 cgttctggaa tatttgttac tttgattgct aaacggcgcg gtgtggaaaa tgtttcgatc
     7321 gtatcataag tcaattggtt ttcatctaaa aattttgctg ttttttcttc taattgtttc
     7381 atgcttggtg tgacaacatg ggcaggcatt tcttccagcc cgatttcaag taataagtct
     7441 tttgccatct tattcagcct ccttcacggt ttctggattt gctgtgtttt tattcaacaa
     7501 agggaatcct aatttttcgc gttctgccac aaagattttg gctacagcac gagccatgtt
     7561 gcggatacgg gaaagataac cggcacgctc tgttacggat actgcaccgc gagcatctaa
     7621 taaattgaat gtatggctgc atttcaaaat ataatcatac gctggatgaa caagattttc
     7681 atcgatacag cgtttagctt ctttttcaaa acgatcaaag ttgcttagta gcaagtcttg
     7741 atcgcttact tcaaaagaat atttggaatg ttcatattct ggttgtttga agatctcgcc
     7801 atatttcacg ccatcagccc attctaaatc atagacactt tcaacttctt ggatataaga
     7861 agcaagacgt tctagtccat aagtgatctc agaagtgact ggtttacaag gcagtccacc
     7921 aacttgctgg aagtacgtga attgtgtgat ttccattccg tcaagccata cttcccaacc
     7981 cacaccggca cagcccatgg atgggttctc ccagttgtct tcaacaaaac ggatgtcgtg
     8041 ttctaatgga tcgattccta aaaggcgcaa gctttctaaa taaagctctt ggatattctc
     8101 aggtgaaggt ttcatcacga cttggaattg atgatgctga tacaaacggt ttgggttttc
     8161 accataacgt ccatcagctg gtcggcgaga tggttctacg taagcagcat tccatggttc
     8221 tggtccgatt gcccgtaaaa aggtatatgg gctcatcgta cctgctcctt tttctgtgtc
     8281 atacgcttgc atcagcatgc agccgtttga tgaccaaaat ttttgtaatg ttaaaatcat
     8341 atcttggacg gtcaattttt tactcatctg ttttgcccct ttcaatttta aagcgttgct
     8401 gtcaaataag aatacaggaa agaaaacgac aaaaaatccc tatgtctgat tacagacata
     8461 gggacgaata attcgcggtt ccaccctact tccgaataaa gattcggcag cttcatttat
     8521 cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg ctcccactct cccgaactcg
     8581 ctaaaccacg tgttttttac tgatcttggt caatgtacgt attcattttg cagttttctt
     8641 gtttctataa tagatcctct cactgaacga tctatttgat ctacggttaa taatagacgg
     8701 aactccctgt gtttgtcaaa tggttttgat tttttgttgt atgtttcatt tactattctt
     8761 cttttttagg tagatctttt tcagacatgt ctttctctgg cgtctttaat acgtgtgccc
     8821 aagacttcat ttgatcgatg aactttttgc ttttcaaatg aataccgaca tattcatcat
     8881 acagctggtc gatgagttca cggattgctt gtttggtttc ttctttcaga tggattccac
     8941 tgattttatc ataagaaata gctgaaaaca tccgaacgaa ataaacagct cgaggatctg
     9001 catgataacg gtgatcgtcc atcgaccaat gtctttcgca tatcacacca ccatatttgg
     9061 aagaatagtc aaattttcct tttgtttccc cacatacgac gcagtgtgtc cagattggtg
     9121 caattccgaa gcgttgcagt atttgaactt caaagatatt cgtgatgatt tctgcatcat
     9181 ctccttgatc catccgctgc agagcttgtt gcgtaaattg gaataaatgc gggtcatatt
     9241 gatgatcttc gattgctgca tctactagat ttagcaaata ggtaccataa ccgctcaaaa
     9301 agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt aaaaacgaca
     9361 aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc aaagccggag
     9421 ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg ccagaagatt
     9481 ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat aaaatcaagc
     9541 ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct ggaaaagcag
     9601 attctgccga tccagattat ttatgtttcg atcttccgcc gtttttctca acgtgcggat
     9661 ttttatccag tttatttcta tacttaacct ataagaagtt cagaatatca gtattcatct
     9721 tttcggtagc cgaagtcttg cagatagact tttttgtctc gccagtcttt ttggactttg
     9781 acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt tcttgctttc
     9841 gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt ttggctatct
     9901 ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg cttcatcgaa
     9961 tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa gactttctca
    10021 cggatcaatt cagaaacgat gaagtattca ggatgatcag tcacttgatc atctggaaaa
    10081 tattgtgggc cctctggcat ttcactgact aatacatcca tcaaacgttc gacgttgttt
    10141 ccttctgtag cagaaattgg aatcacttgt gcaaagggca tctgcgttga ataatcttcg
    10201 atgattccca acaactcgtc tggatggatt ttatcgattt tattgataat cagataaaca
    10261 ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc gcgtttttga
    10321 tcagcgctga tcataaataa gaccgcgtcc acttctttca acgcactgta agctgcttct
    10381 accataaaat ctcctaaacg atgctttggt ttatggattc ctggtgtgtc aataaaaatc
    10441 aactgagctt ctggtgtagt atatactcct tggattttat ttctagttgt ctgcgcctta
    10501 tcactcatga tggcaatttt ttgacccacg attcgattca ataatgtgga ttttcctaca
    10561 ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata cgttctcctt
    10621 tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca acgatgacgg
    10681 caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt ttcccaatcg
    10741 gatgaaaatg aaaatccgta aacatgtcta cgacattttc aaaaacagta ttgatgatct
    10801 ctactatcca tacgagaaaa acagctaaaa gcagccataa ccattctatc cgatcaagtt
    10861 ggaaaatcac gcccataaac aaagcgagga ttccgaacgc aacatgtttc ctcatgttgc
    10921 gttcttcctc aaaaacagtt ttgactcctt ggacggcaaa ttccacggac atcaggaaat
    10981 gtttgttttt ctctacgttt ttatctttta agtccatagg catctaagat ctctttctgt
    11041 aaaccaaaca tttctttctc atcctctggt gtcatatggt catacccgtt gatatgcaag
    11101 aatccatgaa ccgctaaaaa gccaagttcc cgatcatacg aatggccgta agcttctgct
    11161 tgctctgcag cacgttcagt agagatcatg atgtctccca gtacacgagg caatggattc
    11221 tctccatctt caaaaatcac tgggatttcg tcttcaccct cttcttccaa ggcaaaactg
    11281 atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg gatcgctgcg
    11341 ttgtccatga aagtaacaga catttctgtt tcttctggca actctaaata tgatgctgca
    11401 aattgcaaca agtcgtcgat ttctttcatt ttctcttctg agacttggtt tgtttcatcc
    11461 atgaaggtaa tatccattat acatcgcttc tttctattct tcttgctatt cttcttgttc
    11521 ttcttttatc ttctcaactt ccgatttcat tttaggatat ttgatccgag aatggaaggt
    11581 actgcttaaa ccgctgatca atgatttttc tactttacgg atttctttta acgtcaatcc
    11641 actgtcatcc aattgtccat cggaaatacg ctcttctata agattatgga caaaactttc
    11701 gattttttcg cttgttggat ggtccattgc ccgtactgcc gcttcacaac tgtcagctat
    11761 attgacgatt cctgcttcac ggctttgagg ttttggtcca ggataacgga attgctcttc
    11821 cgtgacttcc gggttgcgtt cctttgcctt aacgtagaaa taacgcatca gcgtggttcc
    11881 atgatgctgt ctgcaaatat cgatcaccat ttgcggcata ttataatctt ccaatatttt
    11941 ggcaccatct gtcacatgtc caaagataat atgtttactg tcttctggca ataagaaatt
    12001 gtgcggattt tccgctcctg aaggcaagtt ctcaacgaaa aagctggcgt gtttgatctt
    12061 gccgatatca tgataatagc aagctactcg agttagtaaa gaacgtccac cgatttctgc
    12121 tacagcatta gcacttagat tggctaccat catgctatga tgatacgtac ctggtgcttc
    12181 ttctaacaac tgtttcaata aagggtgatt aggattgctg agttcattta atacgatcac
    12241 actatcatca ttgaccatca attcaatata ggggtgcaga cctatcgtca gcaagaatga
    12301 gaacaatgca cctgtgaatc cgcagactag cgttaaccaa gtcgttgcat ccccaaagct
    12361 cattccttgg tagataatca aaatgacatc catcatcaca gggaaaataa tgacccacat
    12421 agcagcgaat aacccttgtt ctgagatccg tttgcgtctg acgatcgttg ccatgagacc
    12481 agaaaataca taggacatca aaataactgt cagaaagttc gtgccaatcg aatcataaaa
    12541 aatgaaaagt gcggatactg cttggaaaat accagccatg atccctgctc ttcgattcaa
    12601 gaaaaagctt aaaacaagtg gagcaaaagc cgcggggtaa aacagcggaa tataagcagc
    12661 ttgttctgtt tggaagagtt ggaaaaattt cattagtact acgctgactg acattgctgt
    12721 cacgtagaaa agtacgtatt ttgttctttc tcctgcattt tgaaattgaa gtgaattata
    12781 aatcagtaca ccgatttgaa gcaaaacagc taaaaccatg gcaatgagtg ggaaaatcga
    12841 agtcgtttga ttcgtcaatc ctagtaattc caattttttc atcgctgaag catcaatttg
    12901 atttccttcg cgaacaatga tttctccttg gaagatcatc actggttgta cagcttctcg
    12961 tgcagattgt tttaattctt ctgtcttttt ctcattcaat acatcattga ctacaatgcc
    13021 ttgatccact aagtagcgga tcatttgttg ctgctctgtt gtgacattca aatactgaat
    13081 ctgatcttct gcttcttgtt taaaagcttc caattcgctt tcacgaactt gtttactcat
    13141 ctgttcgtca atgagcttca aactttcatc cctaaccgtg tgaatttccg tttcagtcat
    13201 ttcaaatatc gtcgaataaa aattgttcgg cagtttttgg taaaacgcaa cgttttctgc
    13261 attcacgttt tcaaaatttt tcttcagtgt agcaatccgc tcttctgtag taggtttcgg
    13321 gatattttca tcttccttcg ccttatctac cttgtcatta taagatttgt tgatggtatc
//
LOCUS       DACEZN010000063        13370 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEZN010000063 DACEZN010000000
VERSION     DACEZN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.063"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            38..886
                     /locus_tag="IXH60_001479"
     CDS             38..886
                     /locus_tag="IXH60_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990412.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1057..1398)
                     /locus_tag="IXH60_001480"
     CDS             complement(1057..1398)
                     /locus_tag="IXH60_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1990413.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1484..2191
                     /locus_tag="IXH60_001481"
     CDS             1484..2191
                     /locus_tag="IXH60_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1990414.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2178..2501
                     /locus_tag="IXH60_001482"
     CDS             2178..2501
                     /locus_tag="IXH60_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1990415.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2590..3627)
                     /locus_tag="IXH60_001483"
     CDS             complement(2590..3627)
                     /locus_tag="IXH60_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1990416.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4074..5057)
                     /locus_tag="IXH60_001484"
     CDS             complement(4074..5057)
                     /locus_tag="IXH60_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1990417.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5473..6243)
                     /locus_tag="IXH60_001485"
     CDS             complement(5473..6243)
                     /locus_tag="IXH60_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990418.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6236..7171)
                     /locus_tag="IXH60_001486"
     CDS             complement(6236..7171)
                     /locus_tag="IXH60_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990419.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7765..9414)
                     /locus_tag="IXH60_001487"
     CDS             complement(7765..9414)
                     /locus_tag="IXH60_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1990420.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9609..10505)
                     /locus_tag="IXH60_001488"
     CDS             complement(9609..10505)
                     /locus_tag="IXH60_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1990421.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10498..11178)
                     /locus_tag="IXH60_001489"
     CDS             complement(10498..11178)
                     /locus_tag="IXH60_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1990422.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11179..11961)
                     /locus_tag="IXH60_001490"
     CDS             complement(11179..11961)
                     /locus_tag="IXH60_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1990423.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(11954..12871)
                     /locus_tag="IXH60_001491"
     CDS             complement(11954..12871)
                     /locus_tag="IXH60_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1990424.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(12874..13158)
                     /locus_tag="IXH60_001492"
     CDS             complement(12874..13158)
                     /locus_tag="IXH60_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1990425.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         3929 a         2894 c         2357 g         4190 t
ORIGIN      
        1 ctaaatataa ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt
       61 tcctgaaaac gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt
      121 gaactataca agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt
      181 gatcacttgg gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa
      241 aaaatgcaaa cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc
      301 caactactca ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg
      361 ggagctttta acagagattc ttatagaaca tccgcacttt atcgaacgct tcaaagttaa
      421 gatgccattt catgcaataa attattcaga taaattaaca actatcatcc ataaatacga
      481 tcatattatc aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa
      541 gttcatccaa gaagcagttg aatcgcttat tcagcaaaat gcctatcttc ccgcattcga
      601 attgatcaat gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg
      661 aacccttctt ctgatagagg actgttatta tttatggaaa gaattattag cagaagcacc
      721 tcatgcagaa aaaaggcaaa tgttctcttg gttcgtctgc caagtcgatc atactgacgc
      781 ctcttattca aaacgctact ctataaaaat cctgaaagaa gactttcggg aaaaagaatt
      841 ttctaaccaa aagaaaaaaa tagataaaaa actaaagaaa cggtaaagaa agacgatttc
      901 aatgaaaaat agcaagtaaa cgataacaac catcctaaat ccacaagaaa tacagcagac
      961 cttgctttaa aaacacgaat catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca
     1021 atattagtca tttgactatt aggtacgcgt tttgttttat aagtcatgac aaatttcctt
     1081 cgttgctgga tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg
     1141 taagagaggt tccgctgcat ctgtatattg gataaaattc tgacaacaac tttcaggtgt
     1201 gtcgaaccac cctaacccta gtgcagcaat cattgttgcg cctaatgcag gtccttcctc
     1261 catacttaaa caatagaccg ttgcatcgaa aatatcagct tggatttgta gccatgcaag
     1321 atttttcgcg cctcctccca ccgaaacaat acgtgtaaat ctgcgacccg ctagttcttt
     1381 acgtttcaat cccaccattg acaaatgctc attaaaaatt tagaatattc acaagtttaa
     1441 cgacaattat gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga
     1501 tataaaaact gcgatcaaag acacgttacc taccgttttc ggttatatcg gtattggact
     1561 tgcatttggt atcgttggga aagctgccgg atttcatcca ctagtcgtca cgttgatgtc
     1621 cctgctggtc tatgctggtt ctgcccaatt tatcacagtc agcatgcttg ctagtcacag
     1681 cccattgctt tccatcgttt tctcgacctt tctagtcaat tcccgaatga ttctgatgag
     1741 tatgacgatt gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac
     1801 attgctgaca gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa
     1861 gttaaacttt cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc
     1921 ttctcttgtt ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga
     1981 tttcgccatc gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa
     2041 cacctctaaa cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg
     2101 actgattttt atcccaagca acttagtgat tgtagttgta acactgattg ggtgcggatt
     2161 gggggtgtgg atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta
     2221 ctttagcaac ttggctttct agggtcctac catttgtctt actgaaaaaa ttcgatttac
     2281 cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt
     2341 tcagcagtct atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt
     2401 tggcttcagt tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct
     2461 tggcaggaat tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta
     2521 tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg
     2581 tattcttgct tatccgttga acggaccact aacaacgact tttcctaaca tatgtcctgt
     2641 ttctacgtct ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt
     2701 gctcaatgta ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc
     2761 tccttgcgtt tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa
     2821 gctgatagat tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt
     2881 tcccacatgt ccaaatggtc ggacaaggtg cttgatttga tcaaagtagc gggtaatatc
     2941 gaataataca gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa
     3001 gtcgtgatga taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt
     3061 cggacttgca gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga
     3121 accgacgccc ccggatccgt tgatcactaa gatttttttc ccgcgattgg cattttcttc
     3181 tggagttaga ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa
     3241 ggctgcagct tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac
     3301 tagttggtat tcttgattgc ttcctgctct tgttgttgta cctgcgtaaa aaacgcgatc
     3361 gccgacagaa aatttttgca cttcgtcacc tacagcaaca actttcccga ctccatcaaa
     3421 acctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg
     3481 attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg
     3541 ttctttttca tcaataaagc tttttggatc atcgatcggc aacccttcat aaaaaccgac
     3601 taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc
     3661 atccatacag tgttcatttc aatatatata gtatgctatt tgttccacct tttttattca
     3721 tcataattat tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt
     3781 gcgacaagac ttttgtcaca acctcttata aacggtgttc tcctaccaac tcttcggtca
     3841 caagtcaaaa tattcatcaa acatgagaag ctgttttttg aaattgctcc ttgtgactca
     3901 gagttaaacg gcaggctcac aacctctgat ataaacggtg ttcaatcaac tgaccctcgg
     3961 tattagctca aaaaacgaca aaatatgaag agctattttt gtttggctgt tcttattact
     4021 tgatacagga cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt
     4081 tcactgaaaa gcgttggatg ttctttttca aaggtctcta agatctctcg tacttcatca
     4141 gtagatttcg tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata
     4201 tagattttaa agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga
     4261 tcttgcagat ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc
     4321 gggtcttttt caaatgcata aggattcttg aaaatccctc ggccaatcat gatgccatca
     4381 acgccatatt tctcggccaa ttcttctcct gtctggcgat cggggatatc tccattgatc
     4441 gtgattagtg tttgtggagc gatttggtca cgtaaagcaa tgattttagg aatgacttcc
     4501 caatgtgcat ctactttact catctcttta cgcgtacgca aatggacaga aagattggct
     4561 atatcttgat gaagtaggtg actgatccat tcttccatct cagacatttc cgtatagccg
     4621 atacgcgtct tgacgctgac aggcaaaccg ccagctttag ctgcttcaat caattgtgcc
     4681 gctacttctg gacgaaggat caagccactg ccttttccac gttctgctac attagggact
     4741 gggcagccca tgttgatatc gattccttgg aagcccattt ctgccatagc aatgctcatt
     4801 tctctaaaaa attctggctt gtctccccaa atatgggcaa ccatcggctg ttcatcttct
     4861 gtaaatgtta atcttcctcg tacactgtct tttccatctg gatgacagaa actgtctgag
     4921 tttgtaaact cagtaaagaa gacatccggt gctgctgcat gctttacgac atggcgaaat
     4981 acaacatctg ttacatcttc cattggtgct aaaacaaaaa aaggctttgg cagctctttc
     5041 caaaaattag tagtcattta tttgttactc tccattctat caagcagtga agtcgcaatc
     5101 tttctcaaaa acattgtcat tatactagga aacaaaaaaa gaagcaaagt gaacagctta
     5161 aaagatgttt ctgattgagc aaaaaaaaga ctgtgacaaa aatcttatca cagtccattt
     5221 tccggaataa atggtgttca aaagcttgca tttgcggtaa taagtccaac caaacgaaaa
     5281 ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca gctttttcac
     5341 aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattcgctca aaaaacaaca
     5401 aaatatgagg aactattttc ccgtttgttt ctctaatact caggtcaaaa cgctgatttc
     5461 acaacctctt ttctaacttc tcaatcctct tcctttattg atcaaatacc aagtaacggc
     5521 gtacaaaaca acgatgaata acacaagtac gatcatcgat gttgctactg gcacatcgat
     5581 cgttccaagg aatccgtaac ggaaaccgga gatcatataa acgatcggat tgacttttga
     5641 aacagcttgc caaaagggcg gtaacataga gatggcatag aaaacgccac ctagataagt
     5701 taacggctgc aagacaaatg taggcacgat cgatacatca tcataagact gagcaaaaat
     5761 cccgttgagc agtcctgcta atgaaaacaa gattgccgtc atcaataatg taatgatgac
     5821 catgaaccat gaatacacat gaagtggtac aaagaacaag gaaatgatcg ttacgagaga
     5881 gccgacgaga atactgcgac ccacaccacc aatcacaaag ccccaaataa tcacatgcgt
     5941 aggaacaggt gctaccaaga tttcttcgat atttttctga aatttttgag agaagaagga
     6001 cgaagaaaca ttggcatacg aactagtgat agcagacatc ataatcaaac caggaacgat
     6061 gaattccata tatgaaaaac cacccatgtc accgatccgc ccaccgatca tttttccgaa
     6121 aataacaaaa tacaaggatg tcgtgatcac tggcggaacc agtgtttgga cccatatacg
     6181 caaatagcgg ttcgtttcct tgatagctaa actttttaat gcagtaaaat aaagattaaa
     6241 catttttctc ctccgtatga ttattttctt ctgtgatttt caagaacagt tcttctaaac
     6301 gattcgactt attcctcata gaaagaactt tgattccttg tcttgtaagc tgatcgaaga
     6361 ttccgttcac cccttgattt cgttcaactt cgacagcgat tgtctgctca tcttcaaatg
     6421 tacaagcata tccttcaatc acaggtttgg tatcatatgg cgctaaatca aagataaatg
     6481 tttcaaattg cagttttgac agaagctctt tcatacttgt attctcgatc agttcacccg
     6541 attggatgat tccgatgttg cgacaaagca tttcagcttc ttctagataa tgcgtcgtca
     6601 aaatgatcgt tgtaccattt tcgttcaatt ctcgtagaaa ctcccacatc tcgcgtctca
     6661 actcgatgtc aactcctgct gttggttcat ccaagatcag caaacgtggt tcatgcatca
     6721 atgccctagc aatcatcagt cttcgcttca ttcccccgga aagcatacgt gcacggacat
     6781 ttctcttttc ccaaagattg gactgcttca aatatttttc gctgcgtttc atcgcttctt
     6841 tgcgagagac accgtagtag cctgcttgat tcacgacgat ttgctggacc gtttcaaaag
     6901 ggttaaaatt gaactcttgt ggaaccaatc caatctgttg ctttgctcga actagatcct
     6961 tgtccagatc ataaccgaag acattcactt ttccagatgt tttattgact aaagaagtaa
     7021 taatcccgat cgttgtagac ttcccagctc catttggccc taatagcgca tagaaatctc
     7081 cttctttcac cgaaagatcg attccccgca atgcttcgac cccacttcca tagatctttt
     7141 tcaaattatt tatttcaagc gcatttgcca tttatttcct atctccttat agacatgatt
     7201 cttaattgta tcatggaata cagcagatga aaaaacaact tggcttccac acaaaaacac
     7261 acctgctaac aaagaggaga tgtgttttct agttcatgat catcttcgat agaataacta
     7321 tctaaacaac tctggaactt ttaaaatcat aaaagtaccg acagcaacta ctacgatgac
     7381 atatagaaga tccttataac tgaggttctt gtctttttta ttcatgttaa gctttcctcc
     7441 gtccttttat cgaaagcttt tttcttttat tttaaccgaa ataaataaaa tggaaatagt
     7501 tattttcaat ttctgttttc gctgtttgtt ttacggagaa ctttgccatg gaacgtgcgt
     7561 ctcgtcgctg atgctaagtt ttttcgataa aagctgctag aaggaagaca gcgtggttta
     7621 ttttgtatcg tcttttgcta aatcttccaa gatcatctgg acagcagact cgttcccatt
     7681 gaattctgcc acttttttgg catcaatgta cttcttttga taatcgtctt tttggaccca
     7741 cgcatcatgc ttcgtttgat aaagttaatc aaacggatac cactgacgct gttcatcgaa
     7801 caacaaactt tctttttggg cctgttcaac tatggattta ggataattca aaactttcag
     7861 caaggcaatc gcatttcggg tccttgaagg tccttctttt aatttgaaat caaaactgat
     7921 tccttttcct tctgttactt gttcttcaaa gtggacattc tcacaataat tctttaaaat
     7981 ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa gatggatact ccgcaagcca
     8041 ccgaacgact gaagcggaag aagcaatccg ttcgatcgta tttgttcctt ttaaaatctc
     8101 atctacgaag cagtagcagc gttctttgga agccacttgg tccagcaaac gtttgatcga
     8161 tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa tcatcttcga ttgccatgga
     8221 agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca atcgctgttt ggatcgtctg
     8281 tgctagaata cagctgatgg ctatactttt gacatacgta gattttcctg atgcattgga
     8341 acccgtgact aatgtattct tctgccagtt gactggatta actaccgctt cttttaacaa
     8401 tggatgatag atatctttcg ctgtcacacc gccctcagta aactctggtc ggcaagtgat
     8461 cggcatatag gtgcgataat tcaaaacggc tgctgcaact tccaattctc ccagcaattc
     8521 ccacagcttg atggcttcag ttgaatgctt ttctaattta gtgagaacga aatgatagga
     8581 gataaatggc agcatgaaca tgatgttgac atattcaaaa atcaattctg cttcactccc
     8641 gctcttcatt cgaaatgaaa aacctaattt agggatgtct cttaatggtt tagtttgctg
     8701 tttcagctct ttttgaagtg gtgtcgtcat ttttgctaac tgattggcgg ctgaaatcgt
     8761 tgaaaccaaa taactcatgc tattcagctc tgtttctagt gtttgtttct tcactagata
     8821 atagaccgta ttgaatacta agccaccgat tgctagatag attcctagta cttgccctaa
     8881 aagaagcaaa cctacaccca ccagcggaag caagccaaga aaaataaata aacctacgcg
     8941 tccaatcttt ttactttttc catccgctag atattgtttt gcaaaattat gatctttttt
     9001 tcctaaacga gcgaattgat attggattct ttctctcagt tgaggatttt cctgataaaa
     9061 agcaattagt ttttctaact gataatcttc tccaaaatca aagctacgta aacgctgata
     9121 caacgcttct gaacctacac tagaataggt actgttgatc ccttcaaata cttcgaacat
     9181 atccaagtca taccaagtaa gatcgtcgat ttcactatcc catgaacgga attttttctc
     9241 cgtgagccac gcatccttca aactttcttc tttatcaaat ctcggttgat aaggaatttt
     9301 tccccaatta ctacgaactg tttcctttac tttcaaacgt gaaaaaatgt ctaaaacaac
     9361 taataaaata acaactgcaa taataattaa aacgataaat tctctttggc ccatatccct
     9421 ctccgttcct tgatttgtta gttttagtat agcactgttg cgctatttga aaagggggta
     9481 caaaaataac tgtcttcgtt acctaattcg tatcaatcac accaaaataa aaacaccttc
     9541 caaaaaagaa gatgtcttta aaacgagata caattttttg tcattcactt caggtaaatt
     9601 agagccaatt aaaccctccg caacttgacg gagatggaga agatgccccc gtctaatcta
     9661 gcggtcatct ctcctcccat tccttccacc aatttttttg acaggtaaag cccgattccg
     9721 ctagattccg tatttgtgcg gctggtattt tcggtataaa aacgctggaa gactttttca
     9781 acagaggttt gcgctcctgg tttgatagga ttggccactg ttaatcgaat gaatgaatcc
     9841 tcttttgaaa atgtcacctt tgcttgtcca tctgcataac gcaataaatt ctgtatgata
     9901 ttttgcagca ctcgcagcaa catgagcgga tctgctaata cagttgtatt tatcgagtgt
     9961 tcagcgaacg ctacctcgat atttttctca gaaaattgtt cataaaaaga gaaaaatact
    10021 tcttccacct ctttagaaaa agaaagcgta tggagatcca tttctttttg cttcgtctct
    10081 attcgagcaa gatcataaaa ctgatccgtc atactgagta atcttgcaac agattgtttg
    10141 atgatcgtta aatacttctc ccgtttctcc gtatcctcta cctgttccag taattgtaca
    10201 taaccgttaa tagcagtcaa aggagttctg agatcatgag aaaggccgac gatcgatagt
    10261 ctgatgtttt cttccatttc ttgaaatgct tgtccttttt gttcattttc atccacccat
    10321 gtattcagtg catctactag gtccattaga ttcttttctc gaaattcgac tgaaagacga
    10381 ccaccatatt cttgtcgttc agaaagagtg gcgattttct tagttaatct cttgatttcc
    10441 ctttgcagga agaaaataaa acttcccata aagataacaa cgagaagcaa cagccactta
    10501 atcaaatcta aaccccactc cccacaccgt atgaatatac acagcatgtg cctttgctag
    10561 tttccctcga agattggaaa tatgcatatt cactgttttc tcactatcaa aatatggttc
    10621 ttgccaaact gtttcataaa gatttgctcg cgaaaatact tttttcggat gttctaaaaa
    10681 aagcttcagc agatcgaatt ctcttcctgt caaatggaca gccacaccat tcacatttac
    10741 atccctcgac tctttgtcca gtaagatttc cttgtaatag atgcgttgcg acagatcttt
    10801 tacttggaca gaagtctgat gacgaagttg gatgccgatt cgtaacaaca gttcttcgat
    10861 atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa ctttatctct
    10921 ttgatcgctt tttgctgaaa gaacgatgat cggaacattc gatgtctgtc ggatttctgc
    10981 taacaatgct tcacctgaaa gccctggaag catgagatct agaaggatca aatcgacagg
    11041 atatgtgctt aacaataatt ttccttccgt acctgaataa gcatcaagaa gtttgtatct
    11101 ctgttcaaga acctcctcca gtaaagaatg aacagcttca tcgtcttcga taatcaaaat
    11161 agtttccagc aggaacacct acttaatgtc agatttttgg aaaataaaca ttcctagagc
    11221 tgatgatacc acgattgtta ctagactgac cagaatcatt ttcggtgctt gcgaataagt
    11281 tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc gtaaaccact ccgttagaaa
    11341 ttccaagcct ttgatttttg cgaaaacacc gggaacgaag gccgttccca tgatataaag
    11401 aaaagaaaga agcaaactga cactggtact tctagtgatg aatgtcaaag ccactggaac
    11461 agataatgtc gctaatatca gcagaagcaa tacaggaaat acttttagaa cataatttcc
    11521 atagtttttg attccctcaa cttcaccaaa tccgaaagca agtgtgtaag caatcatggc
    11581 tagccccgta atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga
    11641 cacataataa gtcattcgat tccgtcctaa agataacgta ttgcgaatcg taccgttttg
    11701 aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc
    11761 cattttttcc accattaaaa tccctgttgc accgttgatg actgtatccg ttgcttggtt
    11821 taattcattg ccgtattttg tatgcatata ccattcgttc caagaataaa aaagcggaag
    11881 tacgaccatc agtcctagtg agacccacat tttacggctc ttccatactt ttattttttc
    11941 agcagttaat agattaaaca tgtggattgc ctccagttaa ttgtaagaaa taatcttcca
    12001 gtttttgccc aactaggtgg ataccatcca caggaacttt tgcctctacc aatgcttgat
    12061 tgatggctgc tacttggtca attccttcaa aaagctggat aacacggcta gattggacaa
    12121 aataatccct ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt
    12181 caatttttat aaattgacga gattcttgtg ccaattcttc tttcgataat tctttgatca
    12241 agtgtccttc atgcaaaata ccgtagtgag tggcgatctg tgacaattca tctagcaagt
    12301 ggctagaaag caagacggtg atgccccgtt cttttaccaa acgttggatg atttctcgca
    12361 tttctgccat ccctgctgga tccatcccat tcgtcggttc atccaaaatc aaaaattctg
    12421 gattcgtgat cagtgcattt gcaatagcta aacgctggcg catacctaaa gagaaattct
    12481 ttgctttctt tttccctgta ttttccaatc gcatcatttt cagcaggtct tcgatttctc
    12541 tatccgacac accgccgttc gctgcttgga tacgtaggtt atctcttgca gttaattctg
    12601 gataaagagc aggggtttca atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg
    12661 tcgttccaaa taattcgatc gaaccttcat caatcgagat caaacccata atagttctca
    12721 taaaggtaga cttacccgct ccattctccc caatcaaacc atagatcatt ccacgtttga
    12781 tttcgatcga tactttatct aatgctttgg cagcaccgta ttttttggtt ataccagttg
    12841 ctttcaagac cgtttcattt ccaaaatcca tacttatcct tctttcttat tgataagtga
    12901 agtatagttg acgggtataa agaaagcagc cagaaatgta taaagaaagt gtaaattttc
    12961 caattgaatt tcaagaaaaa tcagactgtc tcgcagacgt ggttgagttg tctgccaatc
    13021 aaagagcgca ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat
    13081 cgttgacatc agacgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata
    13141 aaaagcaaaa gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt
    13201 tgggatttac aattagctac caaataagag gttatgacaa aagacttttc acaatccctt
    13261 cttccgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa
    13321 acatgagaag caatttttgt aaattccttc ttatttcttg aagctgacca
//
LOCUS       DACEZN010000064        13278 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEZN010000064 DACEZN010000000
VERSION     DACEZN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.064"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            312..1130
                     /locus_tag="IXH60_001493"
     CDS             312..1130
                     /locus_tag="IXH60_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1990426.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1203..1562)
                     /locus_tag="IXH60_001494"
     CDS             complement(1203..1562)
                     /locus_tag="IXH60_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1990427.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
     gene            1663..1842
                     /locus_tag="IXH60_001495"
                     /pseudo
     CDS             1663..1842
                     /locus_tag="IXH60_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2153..2509
                     /locus_tag="IXH60_001496"
                     /pseudo
     CDS             2153..2509
                     /locus_tag="IXH60_001496"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2522..2932
                     /locus_tag="IXH60_001497"
                     /pseudo
     CDS             2522..2932
                     /locus_tag="IXH60_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3047..3211
                     /locus_tag="IXH60_001498"
     CDS             3047..3211
                     /locus_tag="IXH60_001498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990428.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(3362..4341)
                     /locus_tag="IXH60_001499"
                     /pseudo
     CDS             complement(3362..4341)
                     /locus_tag="IXH60_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4346..5113)
                     /locus_tag="IXH60_001500"
     CDS             complement(4346..5113)
                     /locus_tag="IXH60_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1990429.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            5603..6028
                     /gene="lacA"
                     /locus_tag="IXH60_001501"
     CDS             5603..6028
                     /gene="lacA"
                     /locus_tag="IXH60_001501"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ1990430.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6045..6560
                     /gene="lacB"
                     /locus_tag="IXH60_001502"
     CDS             6045..6560
                     /gene="lacB"
                     /locus_tag="IXH60_001502"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ1990431.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            6571..7503
                     /gene="lacC"
                     /locus_tag="IXH60_001503"
     CDS             6571..7503
                     /gene="lacC"
                     /locus_tag="IXH60_001503"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ1990432.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            7506..8486
                     /gene="lacD"
                     /locus_tag="IXH60_001504"
     CDS             7506..8486
                     /gene="lacD"
                     /locus_tag="IXH60_001504"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1990433.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLGVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            8514..8831
                     /locus_tag="IXH60_001505"
     CDS             8514..8831
                     /locus_tag="IXH60_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1990434.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            8831..10537
                     /locus_tag="IXH60_001506"
     CDS             8831..10537
                     /locus_tag="IXH60_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1990435.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHAGKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            10679..12085
                     /gene="lacG"
                     /locus_tag="IXH60_001507"
     CDS             10679..12085
                     /gene="lacG"
                     /locus_tag="IXH60_001507"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1990436.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12108..12380
                     /locus_tag="IXH60_001508"
     CDS             12108..12380
                     /locus_tag="IXH60_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ1990437.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12401..>13278
                     /locus_tag="IXH60_001509"
     CDS             12401..>13278
                     /locus_tag="IXH60_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1990438.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKI"
BASE COUNT         4255 a         2231 c         2650 g         4142 t
ORIGIN      
        1 tacattcagt aataaggtgc ttatcttagg atcaacttat gatactagct gaccaaggat
       61 gtaccagtta tagaaaatca gttaaagtaa aaatgataaa aatcttatgt caacactgat
      121 ttaggctgac ctgtgggtta ggagaaagac aggtcggtag cttttaatac aaaaaatggc
      181 ttgtatgtat ctagatagtt aactgaaatt agaaatgttc ttatttttaa cggagtaaga
      241 aaaataggct gctttaggtc tgaatacttt ttcaatataa tgaagatatc aaaggcgaca
      301 ggagtgtttt tatgaataag aatcctcata atctaaagtt ggtagcagta gatatggatg
      361 gaacatttgc tcgtactgat tacacatacg atatcccaag gttcaaggct gttttatcgc
      421 gaatgaaagc tgttgattgt caatttgttg ttgcaagtgg aaatcaatac taccaattga
      481 gatcgttatt tccaaattat tatgatgaat taagttttgt tgctgaaaat ggtgcttttg
      541 ttaaagataa aacagaggta ctattttcag ccgatatgcc tagaaaagcg atcgaggtgg
      601 tccttgattt atgtaaagac tatcctgaag taaaaaatgt gatgtgtggt ctgaatagtg
      661 cctactgtga aagaggaaag gtgagtcaag cattttttga tctaaccaat atctattacc
      721 atagattaga atgggtagct gattttgcag aagttcaaga taaaatttta aaatttgctc
      781 cgactatgcc tgttgaaaaa accgaatttt acgttgaatt gttccgtgaa aaattagccg
      841 ggctattagt acctaccagt agtggtcatg gttctgtaga cttgattctt tctgagtgcc
      901 ataaaacttt cggcttgaaa atgttagttg agcgcttggg gattaatcct gaccagtgcg
      961 ttgcttttgg agatgggggc aatgacattg agatgttgga gtattgtggt ctgagttatg
     1021 aaatggataa tgcaacggaa gctgttaagc aagttgctaa gcatcaatgt ccttcaaatg
     1081 acgaagacgg tgttttagtg acgctcgatc gattgttccc taacacgtaa acactaggta
     1141 attcatcaaa aaacctcgat ataagaaata ttcgaggttt taaaactata tttagaacag
     1201 atttacgaag cgtctgatga gtcaaaatag acttggacat tgctatctaa aataccactt
     1261 tcattcttta aacgaattgt ttgatttgat ggagttacct ctaatactaa atgcccatta
     1321 ataaaggtag tatcgtattc agatgtttta actgttgctt cttcgcaagg tattgttaaa
     1381 tcatatgctg gaatataatg ttgtgtggaa actaataaaa aatgatcacc aatcatacag
     1441 catccctctg ctgaagaaaa ttgattagag ccatcattta aagttaacaa gaatccatcc
     1501 tttaaacgag gtacttgctt aaatacttct ggttgtactt ctaaaaataa attatcgctc
     1561 attttcacga ccaccatcga cttccacagt tgaaattgac attttattta acacacctat
     1621 tcccccttaa ctataactca attctatata aaaaatattc tatttcatga ttggctggcc
     1681 caagaaaata tcaattctga gtggttattt ccctcgacag cccattatga tcttcatatc
     1741 actgagaagc agtttcataa ggtgatggcg catgttggtg atctcttagg aatcaacttg
     1801 gaagacaaag agaaaaaatt gcgagtcaga aaaccgtttt gagatatgaa cagcatttgc
     1861 cttcacgcct tagcgatttt aaataatagc cttgaaagtg attatggaaa agccctctac
     1921 gctcaatgca aaattgacgt agaaactatt tttgatcgat tgaaaggtgt atttggtatg
     1981 tgaagaaccc atgtcagagg caagaacgtt gcacctactg acattggaat gatgcttatg
     2041 agcatgaatc taaccaaatt atcccttgag gctagaagaa aagcggaagc ttttcaacac
     2101 aaagcagcca aaaataaaaa acgcgacgaa gcaatcacat ttatgattat tcatcgcgtt
     2161 ttttatattt agagctagtt atttgctaga cccactattt acagatgaaa cgtttatcac
     2221 ctttggtttt gggcatttag tggaattagc agaaccaggt cattatgacg aaaagtggca
     2281 aaattggaaa cttgaatctt tgccgatttt tcctgatcga tacgattttg aagttgcaaa
     2341 agataaggga aagcagttta aaattgttgc agaacttctc aaaaaggcaa atacaattat
     2401 tgttgcaaca gatagcgaca gagaaggtga aaatatcgcc tggtcgatta tccataatgc
     2461 aaatgccttt tcaaaagata aaacatttaa aagactatgg atcaatagct tagaaaaaga
     2521 tgtgatccga agcggttttc aaaatttgca accaggaatg aattactatc ccttttatca
     2581 agaagcgcaa acacgccaaa ttgccgattg gttgatcggc atgaacgcaa gccctttgta
     2641 tacgttaaat ttacaacaga agggcgtaca aggtacattt tcactaggac gtgttcaaac
     2701 gcccacctta taccttattt ttcagcgcca ggaagccata gagaatttta aaaaagaacc
     2761 ttttttcgag gtggaagcta agtaaaacaa gggtcattta agggcgttct aagccccaca
     2821 cagcgtttta aaacccaaga ggagcttttt agcttttgtt tcttctaaac aagctaaaat
     2881 aggcaatcaa gaggggataa ttgctgatgt tcaaaccaaa gagaagaaaa cgaatagtcc
     2941 gagttcgttt tctttaagta gtttgcaatc aaaagtcaat cagctttata aagcgacagc
     3001 gagccaaact ttaaaagcta tgcaaggact gtatgaagca aaattattga gttatccaag
     3061 aacagataca ccatttatta cagagaacga atttgcttat ttaaaagcga attttggcaa
     3121 atatagcggt tttttaggac ttgatcttga aatgttgtta tcattgataa tgatacgttc
     3181 aaacaacagc acccaaattt tgacgaatta gtgaaactct atgaaaaaga cgtagtaaaa
     3241 catgcaacat cctattctaa tcagctggta aagcttaatt aggttatata agcttgaaaa
     3301 taaagggttt gtccaatatg gacaagcctt tttcttatgt tttgccacag atttgccaca
     3361 cttattgtgt aagagtatca aggataggta taatttgata ctttgttttt ttagtgatgt
     3421 gcaaataaat ttctgttaca cgactacctc tagaatgccc tacacggtct tgaattgctt
     3481 ctaggggtat tccttactct gctaagaaac taatatgcgt atgacggaat atatgggtcg
     3541 taatatgttt attaaaacta agtttattat caatatcaat tcctgctcgt tttggattac
     3601 cttttcgatt taattttcca ttttcaggtg agttgaggaa attgttaagg acactataat
     3661 gaagaggtgt tccattatag tcgggtttat gacgactttt ggaagtaaaa atccatccat
     3721 tatctttata atcaggattg aatctcatat gttggtgatt taactcaatt tcctctttaa
     3781 gaatttctat agccctttta gataattgga catttcggta actttgtaag ttttttacat
     3841 taccttcata gctattttct gcagctcctc cgtgagtgga ctgactaatg atgatatttt
     3901 gcttttcaaa atattgatag gcaccaatat tttggagttt cgactctgta agtcaacagg
     3961 gccattagtt agaataccta ttgggatgcg gtttttatgt agagccagca gtagttgatg
     4021 tatctcagga atcatcgtaa tctgatcgag ttcatattca tagtcagctt ggaagtctag
     4081 agcttctgtc ctagaaatac tatcgatgcc aaattcttcc atagtcttct gacagcgaaa
     4141 aattctaagc tgctcaatac tgatgagttt tttcgtgtat ttaggaaaag caacatcaga
     4201 ccaatatctg aaacgtttgt aaatgttatc tatttgttgg atatcaatcg ttggaaaaca
     4261 tttctcaacg ctacgtttaa aggggagttg atggtcataa agagtgtcat caaggtcaaa
     4321 aacaatattt ttataagtca taattctagt ccgcttttaa aatagtagtg tactggctaa
     4381 attcctctaa gtgttgaggg ctaatctggt tatctgtaat gatggtatcg agttgatcta
     4441 gattatagaa gttaaagaaa tcgtatcgat cgaatttagt actgtctact aataagaatt
     4501 tttctacagc attgtttagg gcaagttgtt gaatcacacc ttccttgtca ctatatgtag
     4561 caatagaatt atgggtaaca gcatttgcac taacaaaagc tttggcaaat ctaattgctt
     4621 ttaaatttgt cgaagccatt gaacctacaa aagctccagt tatttctcta tattcaccgc
     4681 caagaagcaa taaatcaatg gtttcgctat catttagaat caagaacaca gggagactat
     4741 ttgtaatgac acgaatttta taacctttac gacctttcaa ttctaatgcc agttgttcaa
     4801 gtgtagttcc aggtccaata aaaatagtat cgccatcttt gattaaagag caggcttttt
     4861 tagcaatgtc aattttttct tttgtattta gacttttctt ctcgatatgt gtcttttcaa
     4921 gtggcttttt agaggaaagc aattgtgcac caccatgagt acgtgttaaa agtccatcag
     4981 cttctagagc atcaaggtct ctacgggctg tcatatcgga aatatctagt tcatctatta
     5041 tttcttttat ggttatggtc ccatcaatct ttaacatatc taaaattttt tctaatcgtc
     5101 gttttttgtt catatgaaga cccttctttc ataaagtaat ttttttccaa agataattct
     5161 cttttaattg tatcataaaa gacaatattt tcaaggtaaa acaaacaatt tcaaacaaaa
     5221 acaaatgtta gatgatgaaa taagaacaga ggattgacgt atattagctt aggtcagatt
     5281 ttgtataaga cgaaaataaa gtaggacctc ttaatcagta agttatagaa agtaaaagac
     5341 ttttgtaata cttgaataga tatttcacgt ccattttgtg atggattaaa tgaacaaaaa
     5401 tgaacaataa tttaacggtg ttatctattt tttaaaaaaa caaataaaaa aaacaaaaaa
     5461 taaacaaaaa aagtgttgct ttttgtttga atgcttgata ttatataaac aaagaaatga
     5521 tgaaaacgtt ttttcgaaca ttttgcaaaa tgttttctac ttctacgtag catttctttc
     5581 taaaatttag gaggtggtca aaatggctat tgttgttggt gcagatctca aaggtacgag
     5641 attaaaagat gttgtgaaaa attttcttgt agaagaaggt tttgaggtaa ttgatgtgac
     5701 taaggacgga caagattttg ttgatgttac cttggcagtt gcctctgaag taaataaaga
     5761 tgagcaaaac ttaggtattg taattgatgc ttatggagct ggtccattca tggtagcaac
     5821 taaaattaaa ggtatggttg cagcagaagt ttcagatgaa cgctcagctt atatgacacg
     5881 tggacataac aattcacgaa tgattacggt tggcgctgaa atcgtcggtg atgagcttgc
     5941 taaaaacatc gctaaaggtt tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg
     6001 agtagatatg cttaacaaaa tgtgttaaga aaaggagtta aggaatgaga atcgcaattg
     6061 gatgtgacca cattgtcaca gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag
     6121 gacatgaggt ccttgatttc gggacatatg accacgcacg tactcactac cctatctatg
     6181 gtaaaaaagt cggtgaagca gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg
     6241 gtacaggtgt cggcattaac aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg
     6301 ttcgtgatat gacatcagct atatatgcaa aagaagagtt aaatgcgaat gttatcggtt
     6361 tcggtggaaa gattactggt ggtcttctta tgaatgacat cattgaagct ttcattgaag
     6421 ctgagtacaa accaacagaa gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa
     6481 cacataatgc acatcaagca gatgaagatt tctttacaga attccttgaa agatgggacc
     6541 gtggtgacta ccacgattaa ggtataacaa atgattctga cagtcacact aaatccttca
     6601 gtagacatct cttatcctct agaaacattg aaaattgata cagtgaatag ggtgaaagat
     6661 gttagtaaga cagctggtgg aaaaggctta aatgttacta gagtcttgta tgaatctggt
     6721 gataaagtta ctgctacagg tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa
     6781 ttagaacaat ctcctgttag tccagctttt tataaaatct caggaaacac tagaaactgt
     6841 attgctatct tacacgaggg taaccaaaca gaaattttgg aacaaggacc aacaatttct
     6901 catgaagaag cagaaggctt tcttgaccac tatagcaatc ttattaaaca atcagaagtt
     6961 gtgacaattt caggaagttt accttcagga cttcccaacg attattatga aaaacttatc
     7021 caacttgctt cggatgaagg agtagcagtc gttttggatt gttcaggtgc acctcttgaa
     7081 acagttctaa aatcgtcggc taaacctact gccatcaaac ctaataatga ggaactttca
     7141 cagttgcttg gaaaagaagt aacaaaagat attgaagaac tcaaagacgt tcttaaagag
     7201 tcacttttta gtggtatcga atggatagtg gtctcattag gtcgaaacgg tgcctttgca
     7261 aaacatggtg atgttttcta taaggtagat attcctgata tcccagttgt taatccagtc
     7321 ggatcagggg actcaacggt agctggtatt gcatcagctt taaatagtaa aaaaagtgac
     7381 gctgacttat taaaacatgc gatgacattg ggtatgttaa atgctcaaga aacaatgaca
     7441 gggcatgtga atatgactaa ttacgaaaca ctaaactcac aaattggagt aaaagaggta
     7501 taaaaatggt acttacagaa cagaaacgca aatctttgga aaaaatttca gataaaaacg
     7561 gtgttatctc agctttggca tttgaccaac gtggtgcttt gaaacgtttg atggcacagt
     7621 atcaagatac agaaccaact gtagctcaaa tggaagaact aaaggttttg gttgctgatg
     7681 aacttacaaa atacgcttct tcaatgttgc ttgaccctga atatggactt ccagcaacaa
     7741 aagcattgga taaagaagca ggtcttcttc ttgcctatga aaaaacaggt tatgatacat
     7801 caagtacaaa acgtttgcct gactgcttgg gtgtttggtc tgcaaaacgt atcaaagagc
     7861 aaggcgctga tgccgttaag ttcttgcttt actatgatgt agacagctca gatgaactta
     7921 accaacaaaa acaagcttat attgaacgtg ttggttctga atgtgtcgct gaagatattc
     7981 ctttcttctt ggaaatcctt gcttatgatg aagaaatctc agatgcaggt tctgttgaat
     8041 atgcaaaagt gaaacctcgc aaagtcattg aagcgatgaa agttttctct gatccacgct
     8101 ttaacattga tgttcttaaa gtagaagtac cagttaacgt taaatatgtc gaaggttttg
     8161 ctgatggtga agtagtttac agtaaagctg aagctgctga cttctttaaa gcacaagaag
     8221 aagcgactaa ccttccatac atctacctaa gtgcaggtgt atctgctaaa ttgttccaag
     8281 aaacattgca atttgctcac gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg
     8341 ctacttgggc aggatctgta gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat
     8401 ggttgcgtac tactggattc gaaaatattg atgaacttaa caaagttctt gttaaaacag
     8461 ctagtccatg gactgataaa gtatagtaaa aacataaaac ggaggatatt gttatgaaca
     8521 gagaagagat gactctctta gggtttgaaa ttgttgctta tgctggagat gctcgctcta
     8581 aacttttaga agcgcttaaa gcggctgaaa atggtgattt cgctaaggca gatagtcttg
     8641 tagtagaagc aggaagctgt attgcagagg ctcacaattc tcagacagct atgttggctc
     8701 gagaagcttc tggggaggaa cttccataca gcgttaccat gatgcatggt caggaccact
     8761 taatgactac tgtcttatta aaagatgtga ttcatcacct catcgaactt tataaaagag
     8821 gagcaaaata atgcataaac tcattgaact tattgagaaa gggaaaccgt tctttgagaa
     8881 aatctctcga aacatctatc ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt
     8941 catccttttc tcatctatct ttatccttat tgcctatgtc ccaaatgctt ggggattcca
     9001 ctggtcaaaa gacattgaga ccttcttgat gactccttat agctattcga tgggtatcct
     9061 agcattcttt gttggtggta ctaccgctaa agctttgacg gactcaatga accgtgacct
     9121 acctgcgacc aatcagatta acttcttatc tacgatgcta gcgtctatgg tagggttcct
     9181 tttgatggca gctgagcctg caaaagaagg aggcttcttg acagccttca cgggaacaaa
     9241 aggtcttttg acagcattta ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt
     9301 gaaaaataat gttactattc gtatgcctga agaggttcca ccaaatatct ctcaagtatt
     9361 taaagacttg attccattca ctgtagcggt tgttctcctt tatggatttg aactcattgt
     9421 taagggaact cttggtgtaa ccgttgcaga atcaatcggt acccttcttg ctcctctctt
     9481 ctcagctgca gatggttacc ttggaattac acttatcttt ggtgcttatg ccttcttctg
     9541 gtttgttggt atccacggtc cttcaattgt cgaaccagca atcgctgcta tcacttatgc
     9601 caatatcgat gccaacttgc atcttatcca agctggacaa catgcaggca aagttatcac
     9661 ttctggtact caaatgttta ttgttaccat gggtggaaca ggagctacat tgattgttcc
     9721 attcttgttc atgtggattt gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt
     9781 tgttccaaca ttctttgggg ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa
     9841 tccgattttc tttgtaccgt tcattttcgc tccgattgtc aacgtttgga tctttaaatt
     9901 ctttgttgac acattgaaca tgaactcatt ctctgccaac cttccatggg ttactcctgg
     9961 tccgttggga attgtactcg gtactaactt ccaagttcta tcatttatct tagccggact
    10021 cttggtagtt gttgatacta tcatttatta cccatttgtt aaggtatacg atgaacaaat
    10081 tcttgaagaa gaacgttctg gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa
    10141 cttcaatact gctaaagcag atgccgttct tggaaaagca ggcgttgcta aagaagatgt
    10201 agcagcaaac aataatatca caaaagaaac taatgtcctt gttctttgtg caggtggagg
    10261 tacaagtggc ttgcttgcca atgccttgaa caaagcagca gcagaataca atgttccagt
    10321 caaagcggca gcgggtagtt acggtgctca ccgtgaaatg ttgccagagt ttgacttggt
    10381 tatcttggct ccacaggtcg cttcaaactt cgatgatatg aaggctgaaa cggataaatt
    10441 gggcattaaa cttgcaaaaa ctgaaggagc tcaatatatc aaattgacac gtgatggaca
    10501 aggtgctctt gcatttgttc aacaacagtt tgattaataa atttgtaaaa gttatttgag
    10561 acgagattat tctttcaatt ccttataata ttgttaacca tatcattgca tttcatttcc
    10621 ttggtttccg gaactcagaa tatcgtctca aatgcttttt ttgaaaggat ttatagttat
    10681 gactaaaaca cttcctaaag attttatttt tggtggtgct acagctgctt atcaagcaga
    10741 aggtgcgact cacacggatg gtaaaggccc agttgcatgg gataagtatc ttgaagacaa
    10801 ttattggtac acagcagagc ctgcaagtga tttttaccat aaatatcctg ttgatttaga
    10861 attagctgaa aaatatggcg ttaatggtat tcgtatttca attgcctggt ctcgtatttt
    10921 cccaactggc tatggcgaag taaatgaaaa aggtgttgaa ttctaccata atctttttgc
    10981 cgagtgccac aaacgtcatg ttgagccatt cgtgactttg caccactttg acacaccaga
    11041 agttcttcac tcagatggag atttcttaaa ccgcgaaaat atagaacact ttgtagatta
    11101 tgccgctttc tgttttgaag agttcccaga agtaaactac tggacaactt tcaatgaaat
    11161 tggcccaatt ggagatggtc aatacttggt tggtaaattc cctccaggca ttcaatatga
    11221 tcttgccaaa gtcttccaat cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa
    11281 attgtataaa gataaaggtt ataaaggcga aattggtgta gtacatgcgt tgccaaccaa
    11341 atacccttat gatccagaaa atccagcaga tgttcgtgcc gctgagttag aagatatcat
    11401 ccataacaaa tttatcttgg atgcaaccta ccttggacac tattcagata aaacgatgga
    11461 aggggtcaac cacatcctag ctgaaaatgg tggagaactt gatcttcgtg atgaagactt
    11521 ccaagcactt gaagcagcta aagatttgaa cgatttcctt ggtatcaact actacatgag
    11581 tgattggatg caagcctttg atggtgagac tgaaatcatt cacaatggta agggtgaaaa
    11641 aggaagttct aagtaccaga ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc
    11701 tagaacggat tgggactgga ttatttatcc tgaaggcttg tatgaccaaa tcatgcgagt
    11761 gaaaaatgat tatccaaact acaagaagat ttacatcact gaaaacggtc ttggatacaa
    11821 agatgagttt gtagataata ccgtttatga tgatggtcgt attgattacg tgaagaaaca
    11881 cttggaagtt ttatcagacg caattgcgga tggtgcaaat gttaaaggtt acttcatctg
    11941 gtcacttatg gatgtcttct catggtcaaa cggatacgaa aaacgttatg gactattcta
    12001 tgtagacttt gacacgcaag aacgctatcc taagaaatca gctcattggt ataagaaagt
    12061 agcagaaact caagtgatag agtaatatta cttgaaaatg taaaaaattg ttatccgata
    12121 ctttagaaaa agtacaacac ttatttagac caggctttga aaaggtatat attgttagtt
    12181 tcgaagattg tcctatgatt cctgagcttg aagctacccc gttgctaaaa tgtggtaaat
    12241 ggtatgtttc aactggaaaa gagtggattt gccattctga tttagagtta tcagcatttg
    12301 aatgggaatt tttacaatca ctcgatgtag aaatacgtga gactattcac ttcgaggtaa
    12361 attatttgcc tttccaataa taaaaagaaa ggaattagtt gtgactatcg aattaaaaaa
    12421 cgagtatctt actgttcagt ttaaaacact tggtggtcaa ctgacttcga ttaaagacaa
    12481 ggatgggata gagtatctct ggcaagctga tccaaattat tggaatggac aggcgcctat
    12541 tctatttcct atctgtggta gtttacgaaa tgattgggct atttatagac ctcaagaaag
    12601 accctttttt acaggtctta ttcgtagaca tggttttgtt cgaaaagaag aatttattct
    12661 ggaagaagtc aatgataata gcgtgacctt tagtattaaa ccaaatgctg agatgcttga
    12721 taattaccta tatcagtttg aactgagagt tgtttatacc ttgaatggta aatcaattag
    12781 aaccgaattt caggtaacga acttggaaat ggaaaaaaca atgccttact ttattggagc
    12841 ccatccagca ttcaatagtc ctttagtaga aggtgaaaac tatgaggatt attatattga
    12901 atttagtgag gttgagtctt gctcgatacc aaagagtttt cctgagacag gattattaga
    12961 tttgcaagat cggacacctt ttttggaaaa tcaaaaaatt ctcgatttgg actactcact
    13021 tttttcccat gatgccatta cacttgacca gctcaagtct cgtagcgtaa cgcttcggtc
    13081 aaggaagtct ggtaaaggtc ttcgagtaga ttttgatgat tttccgaact tgattctgtg
    13141 gtctacaact aataaaagtc cattcattgc tttggaacct tggagtggtc tctccacttc
    13201 acttgaagaa ggtaatttct tagaagataa aagacaggta acgaaaattt cacctcaaga
    13261 aacttctaga aaaatcta
//
LOCUS       DACEZN010000065        13255 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEZN010000065 DACEZN010000000
VERSION     DACEZN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.065"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(109..2235)
                     /gene="pnp"
                     /locus_tag="IXH60_001510"
     CDS             complement(109..2235)
                     /gene="pnp"
                     /locus_tag="IXH60_001510"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1990439.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2423..2692)
                     /gene="rpsO"
                     /locus_tag="IXH60_001511"
     CDS             complement(2423..2692)
                     /gene="rpsO"
                     /locus_tag="IXH60_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1990440.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2943..3506
                     /locus_tag="IXH60_001512"
     CDS             2943..3506
                     /locus_tag="IXH60_001512"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1990441.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3759..4280)
                     /locus_tag="IXH60_001513"
     CDS             complement(3759..4280)
                     /locus_tag="IXH60_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1990442.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4283..5125)
                     /locus_tag="IXH60_001514"
     CDS             complement(4283..5125)
                     /locus_tag="IXH60_001514"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1990443.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5118..5843)
                     /locus_tag="IXH60_001515"
     CDS             complement(5118..5843)
                     /locus_tag="IXH60_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1990444.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6019..6315
                     /locus_tag="IXH60_001516"
     CDS             6019..6315
                     /locus_tag="IXH60_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990445.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            6883..8475
                     /locus_tag="IXH60_001517"
     CDS             6883..8475
                     /locus_tag="IXH60_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1990446.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8488..9228
                     /locus_tag="IXH60_001518"
     CDS             8488..9228
                     /locus_tag="IXH60_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990447.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9396..10745
                     /locus_tag="IXH60_001519"
     CDS             9396..10745
                     /locus_tag="IXH60_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1990448.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(10873..11463)
                     /locus_tag="IXH60_001520"
     CDS             complement(10873..11463)
                     /locus_tag="IXH60_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1990449.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            11610..12962
                     /gene="brnQ"
                     /locus_tag="IXH60_001521"
     CDS             11610..12962
                     /gene="brnQ"
                     /locus_tag="IXH60_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1990450.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         3721 a         2619 c         2611 g         4304 t
ORIGIN      
        1 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
       61 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
      121 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
      181 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
      241 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
      301 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
      361 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
      421 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
      481 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
      541 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
      601 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
      661 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
      721 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
      781 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
      841 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
      901 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
      961 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
     1021 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
     1081 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
     1141 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
     1201 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
     1261 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
     1321 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
     1381 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
     1441 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
     1501 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
     1561 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
     1621 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
     1681 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
     1741 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
     1801 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
     1861 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
     1921 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
     1981 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
     2041 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
     2101 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
     2161 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
     2221 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
     2281 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
     2341 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
     2401 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
     2461 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
     2521 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
     2581 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
     2641 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
     2701 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
     2761 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
     2821 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
     2881 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
     2941 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
     3001 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
     3061 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
     3121 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
     3181 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
     3241 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
     3301 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
     3361 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
     3421 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
     3481 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
     3541 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
     3601 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
     3661 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
     3721 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
     3781 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
     3841 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
     3901 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
     3961 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
     4021 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
     4081 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
     4141 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
     4201 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
     4261 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
     4321 gtttcatcaa atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca
     4381 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
     4441 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
     4501 ccaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
     4561 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
     4621 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
     4681 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
     4741 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
     4801 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
     4861 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
     4921 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
     4981 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
     5041 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
     5101 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
     5161 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
     5221 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
     5281 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
     5341 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
     5401 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
     5461 tttcgacaat gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc
     5521 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
     5581 tgtgctattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
     5641 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
     5701 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
     5761 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
     5821 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
     5881 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
     5941 gcaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
     6001 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
     6061 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
     6121 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
     6181 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
     6241 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
     6301 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
     6361 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
     6421 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
     6481 ttttcaaaaa ccagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
     6541 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
     6601 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
     6661 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
     6721 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
     6781 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
     6841 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
     6901 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
     6961 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
     7021 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
     7081 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
     7141 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
     7201 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
     7261 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
     7321 gcaaaaagtt tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac
     7381 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
     7441 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
     7501 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
     7561 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
     7621 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
     7681 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
     7741 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
     7801 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
     7861 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
     7921 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
     7981 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
     8041 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
     8101 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
     8161 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
     8221 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
     8281 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
     8341 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
     8401 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
     8461 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
     8521 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
     8581 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
     8641 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
     8701 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
     8761 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
     8821 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
     8881 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
     8941 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
     9001 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
     9061 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
     9121 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
     9181 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
     9241 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
     9301 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga
     9361 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
     9421 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
     9481 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
     9541 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
     9601 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
     9661 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
     9721 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
     9781 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
     9841 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
     9901 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
     9961 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    10021 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    10081 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    10141 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    10201 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    10261 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    10321 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    10381 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    10441 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    10501 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    10561 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    10621 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    10681 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    10741 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    10801 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc
    10861 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    10921 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    10981 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    11041 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    11101 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    11161 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    11221 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    11281 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    11341 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    11401 aataactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    11461 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    11521 accatcgttt tatataggga attttctgaa aattgttgca tatttttcga aattttgtta
    11581 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    11641 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    11701 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    11761 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    11821 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    11881 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    11941 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    12001 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    12061 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    12121 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    12181 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    12241 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    12301 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    12361 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    12421 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    12481 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    12541 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    12601 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    12661 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    12721 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    12781 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    12841 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    12901 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    12961 gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa
    13021 tggcttctca catttttgca taaatttgac ttaataccga gaaactggct tttgaacacc
    13081 gtttattgag cgtctataaa ctgacctgag tattagagaa acaaaacggc aaagtagctc
    13141 catatacttt gccgttttgt tttgaactaa taccgaagag aagctgatca aacaacattt
    13201 cttttataat atagatttta gacatatata tgatataaaa atagttttcg caaaa
//
LOCUS       DACEZN010000066        13159 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEZN010000066 DACEZN010000000
VERSION     DACEZN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.066"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            171..515
                     /locus_tag="IXH60_001522"
     CDS             171..515
                     /locus_tag="IXH60_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1990451.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IXH60_001523"
                     /pseudo
     CDS             516..935
                     /locus_tag="IXH60_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="IXH60_001524"
     CDS             complement(1027..1377)
                     /locus_tag="IXH60_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990452.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IXH60_001525"
     CDS             complement(1343..2674)
                     /locus_tag="IXH60_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1990453.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IXH60_001526"
     CDS             2930..3496
                     /locus_tag="IXH60_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1990454.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IXH60_001527"
     tRNA            3641..3728
                     /locus_tag="IXH60_001527"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /locus_tag="IXH60_001528"
     CDS             4062..5036
                     /locus_tag="IXH60_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1990455.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="IXH60_001529"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="IXH60_001529"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1990456.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="IXH60_001530"
     CDS             complement(7451..7897)
                     /locus_tag="IXH60_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1990457.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="IXH60_001531"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="IXH60_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1990458.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="IXH60_001532"
     CDS             8362..8880
                     /locus_tag="IXH60_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1990459.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="IXH60_001533"
     CDS             complement(8957..9913)
                     /locus_tag="IXH60_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1990460.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="IXH60_001534"
     CDS             10079..10885
                     /locus_tag="IXH60_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990461.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="IXH60_001535"
     CDS             10882..11871
                     /locus_tag="IXH60_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1990462.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="IXH60_001536"
     CDS             11868..12872
                     /locus_tag="IXH60_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1990463.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..>13159
                     /locus_tag="IXH60_001537"
     CDS             13004..>13159
                     /locus_tag="IXH60_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1990464.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTL"
BASE COUNT         4242 a         2250 c         2641 g         4026 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaaaaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attgttcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 catacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcagc caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatat cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt aagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga tcttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaatcga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgacttta
//
LOCUS       DACEZN010000067        13145 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEZN010000067 DACEZN010000000
VERSION     DACEZN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.067"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(77..149)
                     /locus_tag="IXH60_001538"
     tRNA            complement(77..149)
                     /locus_tag="IXH60_001538"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IXH60_001539"
     CDS             530..1099
                     /locus_tag="IXH60_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1990465.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IXH60_001540"
     CDS             1086..1946
                     /locus_tag="IXH60_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1990466.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IXH60_001541"
     CDS             2136..2840
                     /locus_tag="IXH60_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1990467.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IXH60_001542"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IXH60_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1990468.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IXH60_001543"
     CDS             4677..5993
                     /locus_tag="IXH60_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990469.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IXH60_001544"
     CDS             5994..6863
                     /locus_tag="IXH60_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990470.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IXH60_001545"
     CDS             6918..7727
                     /locus_tag="IXH60_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1990471.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IXH60_001546"
     CDS             complement(7830..8459)
                     /locus_tag="IXH60_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1990472.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IXH60_001547"
     CDS             8629..9921
                     /locus_tag="IXH60_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1990473.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IXH60_001548"
     CDS             complement(9997..10659)
                     /locus_tag="IXH60_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1990474.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IXH60_001549"
     CDS             10884..11168
                     /locus_tag="IXH60_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1990475.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IXH60_001550"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IXH60_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1990476.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2264 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccatt
//
LOCUS       DACEZN010000068        13086 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEZN010000068 DACEZN010000000
VERSION     DACEZN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.068"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..885)
                     /locus_tag="IXH60_001551"
     CDS             complement(<1..885)
                     /locus_tag="IXH60_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990477.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQI"
     gene            complement(1093..2019)
                     /gene="glsA"
                     /locus_tag="IXH60_001552"
     CDS             complement(1093..2019)
                     /gene="glsA"
                     /locus_tag="IXH60_001552"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1990478.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(2042..3253)
                     /locus_tag="IXH60_001553"
                     /pseudo
     CDS             complement(2042..3253)
                     /locus_tag="IXH60_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(3288..4901)
                     /locus_tag="IXH60_001554"
     CDS             complement(3288..4901)
                     /locus_tag="IXH60_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1990479.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(5013..5222)
                     /locus_tag="IXH60_001555"
     CDS             complement(5013..5222)
                     /locus_tag="IXH60_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1990480.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            5313..5501
                     /locus_tag="IXH60_001556"
     CDS             5313..5501
                     /locus_tag="IXH60_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990481.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            complement(6211..7425)
                     /locus_tag="IXH60_001557"
     CDS             complement(6211..7425)
                     /locus_tag="IXH60_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1990482.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(7553..8023)
                     /locus_tag="IXH60_001558"
     CDS             complement(7553..8023)
                     /locus_tag="IXH60_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1990483.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(8020..8262)
                     /locus_tag="IXH60_001559"
     CDS             complement(8020..8262)
                     /locus_tag="IXH60_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ1990484.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(8808..9338)
                     /locus_tag="IXH60_001560"
     CDS             complement(8808..9338)
                     /locus_tag="IXH60_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1990485.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(9520..10176)
                     /locus_tag="IXH60_001561"
     CDS             complement(9520..10176)
                     /locus_tag="IXH60_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1990486.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(10303..10776)
                     /gene="dfrF"
                     /locus_tag="IXH60_001562"
     CDS             complement(10303..10776)
                     /gene="dfrF"
                     /locus_tag="IXH60_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1990487.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(11195..11413)
                     /locus_tag="IXH60_001563"
                     /pseudo
     CDS             complement(11195..11413)
                     /locus_tag="IXH60_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11444..12658)
                     /locus_tag="IXH60_001564"
     CDS             complement(11444..12658)
                     /locus_tag="IXH60_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1990488.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(12999..>13086)
                     /locus_tag="IXH60_001565"
     CDS             complement(12999..>13086)
                     /locus_tag="IXH60_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1990489.1"
                     /translation="TIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT         3868 a         2757 c         2004 g         4457 t
ORIGIN      
        1 aatctgtaaa atcaattcat tcagtgatag gtcattgaga acaaaatttt tataaacaac
       61 ttcgaaatct tttcttaaca gctccatgtt ctcattggta taatttctac tgttgaacaa
      121 agaaaaaata taatagacat cttcttcttg taagtctaga attccttttt gctcttccaa
      181 tccttgacag actaattgaa ataacggctt cgtttctgcg cttttcttat cttcttcttt
      241 cagtccaagg ggaaatgcct ttcttccgat tgcgatctct atttctcgta tgataaagga
      301 cttttcatct tccgaatatc ttcttttaaa aaaatgagtc tctacataat caatcaactc
      361 gttacaagct tcttctacat acggattttt attttcatag ccttcccaat tgacataacg
      421 tactaaagct agcaagatgt tccgcgagtc aaattctggg ataatgattt ctttattttt
      481 caaataatca ttttctttaa aaaaatccaa tacgattttt ttgattgtat atactttact
      541 caaagaaata aattcaagat cagccagttc tgtactcgtg aatataccat taaaataata
      601 acttaaaaac tttaaaaaat aagattgacc gtacagaaac tgtgtgagct cagaaagagc
      661 aataccatgt ttgaatacga gtttatactt atgcttctct cttacttgtt cttttataca
      721 atattctaga cactcggcta gactttctag atcgtgaagg atcgttggtg tagtcaccct
      781 tagagaaata gctgtttgtt cgatgtctat ttcttggaat tcaaataatt gttcacacag
      841 atacactttt ctaaatatat ttcgctcgat gtaattttcc agcattcctg atacacgcct
      901 ccagtttttc ttatgatttt atttaatcat atctgtaaat cgtatttttg tttccttttt
      961 atttagaaaa gccctttcaa aaaccaataa aaaaagaaac tgggacaaaa gtcaaacaag
     1021 ctcacaattt cagcctcatt gtaggtagct ttgagccgtt tggactttta ccccagcctc
     1081 tttcaagaat ggttattcaa agatgtcagc gtctagttct ttgactacat ctttcaacaa
     1141 ttggatacca gctgcgctgt taccaaatgg atctagtgcc gggctgaaga cacctatgcc
     1201 gtaacgattt ggcacagctg ccatcaaccc gccgcctaca ccgctttttg caggtacacc
     1261 aacatggacg gaaaactctc ctgattcatc atacaaacca gcagttgtca taattgattt
     1321 gacgattgta gcactttctt cagtaatgat ttgtttgcca ttccaaggag caattccttt
     1381 atttgccagc acagcagcta attttgctat gcctgtagcg gtgacattga gggagcattg
     1441 tctgaaataa gcatcgagta tctccggtac gtctccttcg atcatttgat tacctttcat
     1501 atagtaagca agagagcgat tgatatcacc tgttcttgat tcagaatgat agatttcttc
     1561 attcaatgtg atattaggat catcacagat ctctttcata aatgacaaga ttcgctcaaa
     1621 tttttcatct gcatctttac cggcaatcaa cgaggttgtt gcaatcgctc ctgcattaac
     1681 aaaaggattc attggatgtt tgcgatgatt gatttccata ttcatgatag aattgaaggc
     1741 aaaaccggtt ggttccgtgt tgattgtttg aaagactttt tctattccgt tgtctttgat
     1801 tgctaataac aatacgggaa cttttgacat gctttcgatg gcaaagcgca catcggcgtc
     1861 tcctccctca atcagttgtt ttgtatccaa atcataaatc gccataccta attgtttggg
     1921 atccacttca gctaatgctg gaatgtaatc cgccacttgt cctgtttgat aatagtggaa
     1981 attttttcca atcaattgtt ctaattcttt tacttccatt tgaataacca gctttcattt
     2041 tttatttttc tgcatgctgg aatttatgac ggcatgcgta gatcgtaaat ggaataacta
     2101 agacgataag ataagaaaca atcagaatcg tttcataaat cccatgctgt ccagctgcga
     2161 tactagcagg tggaacaaat gagatacaaa aagcaaaaag tgaagtcaaa agacctaaac
     2221 aagcaacgat tacttttaca acttttccgc ctggaacctg ataggctctt tcaagactgt
     2281 catctttaaa aaccagcttc aaatagcccg cgaaaaacat aaagtacaac atcaggtaga
     2341 ttacaacagt caatgacatc gctgcaagaa atgaaagatt attccccccg ccgcttaatg
     2401 taaagacggc tgcccagatt gttgcaacga tcccatttgc gatcatcaaa tgagcaggaa
     2461 caccgtgtgt gttttctttt gcaaaatatt caggaagtaa tccttcttct gctgctacgt
     2521 gaagtgcttt tgaaggagag gttacccaag aagcaatttc tccgatgaca cctaaacaaa
     2581 caagaatcgc aagaattttc gcaatccatg caagatttgg atcaatgtgt cttaatagga
     2641 attctaacga ttgaatcacc cctgtattaa gagacaaacc atgttgaggt actgtagtag
     2701 cgacggtcaa tccgccaaaa gtatctaaaa tgattgccag aataaccaat acaaacattg
     2761 ctaatggata atccttttta gggttttcca tttcatttgc atgactggca gatgtttcta
     2821 cgcccatata tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat ttcgaaaaat
     2881 caggaatcaa tgttttcata gacagtatcg tttgtagtgg agcgcctgaa gcgacataat
     2941 ggatccctaa gccaaacaag actaatgctg gcaacaagat accagctaca aaaccgatcc
     3001 gagcaatcaa tgaagtgtat ttggttcctt ttaactgtga gagtaaaata gcccagaaaa
     3061 taatcaatag gattgccaat ttcagatatt tattgttttg tatagcacca atacccgttg
     3121 cataggataa tgcaccagtt aagaaataca gcatggtcaa gtagccaact gttatctcaa
     3181 accattggaa aaagattgcg gcaaaaccga atcgttttcc caatgtgcga ctgacccaag
     3241 tatagacacc gccggtttcc cttagttaaa aatattactc ttgttgatta ctcaactctt
     3301 ccaataaatc ggtaatatat ctcaatttcc tgtattcttt cgttatcctc attcgttgtt
     3361 gcttcatgta tgaggatttt ttctatcatg gcatttaata atggtgctgt cagttccttt
     3421 ggtacggaat attccttgat taactcaatc cacttttcag cacctatcat atcctgttcc
     3481 attttactta gttcttctct taggcttgtt atctgctgtt ccagttctat ctgttctttt
     3541 tggtacttgc tggacaacat cacaaaattt cgctctgtta tcttctcaca ggctctatct
     3601 tcatacattt tcgcaaataa acggtcaatc tcattttgtc tgttctcggc tttcttcaat
     3661 gtacttgcct ttttcttctt ttcccgtatt ctctctgcat tgccagcttt ctgtatcttg
     3721 ttcaatacct tttcttcgtc ctgctgtact gccttagccc aatactgcaa gcgttccaat
     3781 acggcttgat acagcacatc ataacgaatg tagtgcatag aacaattacc tttgccaaac
     3841 tgcccgtagt aactgcaagc ataataactg taaggcgttt tatttgtctt attcgttcca
     3901 aatctcatag accaaccaca atccgcacac ttgacaagcc ctgcaaaaat cggcgttgcc
     3961 ttttcctttg tctgtctgcg tcttgatttt atctgctcct gcacacgata gaacacttcc
     4021 ttgtcaataa tcggctcgtg agtgttttct actcgaaacc attcactttc gggcttacgc
     4081 actttctttt tgctcttgaa cgataccgta gactgcatat tgtgaacact gtttccgata
     4141 taaacctcac ttttcaatat cgccttgaca tgagcaatcg tccactcata acgcttactt
     4201 tcgggttttc cctcaaagat atgtgcaaaa gtaccgtatc ttgtaaaatt tagccatgac
     4261 gctgtcggaa ctttttctgc tcttaactgc tttgtgattt ttgcacttcc gtaaccttga
     4321 taggctaggg aaaagatttt ttcaataatc cattttgttt catcatcaac caacagtttt
     4381 cctttgatgt cagggtgttt cttatatcct aacggagcat aagcaccgta atgagccccc
     4441 tccgcaaact ttgtcttaaa tgctgatttg actttacggc ttgtatctct tgccacccat
     4501 tcattgatga tgttcttaaa tggtgcaatc tcactttcgc ctttttcgct gtctacaccg
     4561 tcatcaatcg ctatgtagcg gacattatga ctagggaaat acaattctgt atattgtcct
     4621 gtcataatat aatttctgcc aagacgggaa aggtctttcg ttacaacaca gttgattttt
     4681 cctgcctcta tatcctcaat cattctttga aaactcggtc tgtcaaaatt tgttcccgac
     4741 cagccgtcat caatatattc atcaatgaca ttcaaatgat gttcttttgc atataaacgc
     4801 aacatacttc tctgtgtggt aatgctggaa ctttcgccct gtaattcatc atctcggctt
     4861 aacctaagat aaagtgcagt attgtaaatc tgttgtttca ttgtcagtta tcctccttta
     4921 taactaaccc gtgtttacaa tacctgcttg caagtaaagt ataacacggg tactttttgt
     4981 cattcaatct atttcttaca actgcaccga ttttatgcga ttgtctgctt tgctttttcc
     5041 agcatgacat cagttagaag ttcttccatt gttttgccct tttcggaaaa atgctctttt
     5101 actacaatct ttgtctttcc tctgttgcca atgccacact tgccattttt cttttgaatt
     5161 gtttttacag cattatcaat aacacatacc ccctttctgt aaaatctttc taagcaattc
     5221 aacggctttt gtcatagccc acaggagttc cacctctgca tagttcttat gtagccgtac
     5281 ccattgcgtg cgacgctctt aatgctcaag ctgtggctgt acgggagtat cattatctga
     5341 cagtacaggt catggcgata aaagaggaca aatcttttac aagaacacaa ataggttttc
     5401 gctcgctctt gcaggggaaa ggtcgtggcg tatttgtttc aacggctagc tgtctgtcaa
     5461 acgtattgaa tagctttatt cagttgtcaa agaacgattg aaagaaaaaa atcgtctttc
     5521 ctatatatcg cgttggaaaa gaggagaaac gtaaccaaaa tccaaaactt tttccattat
     5581 tagtacaaat ggaacggttc attttggcaa tcaacggttg attttctcct ttttcactac
     5641 tatctgtaca gcaaacgtcc atttgctaag tttaaatgga aaaattttaa cttcttttca
     5701 tgcaccatat agggatagaa aatgtcattt tgctaagttg atgatgaaaa aagaatattt
     5761 tccttttcac accctaacta cccaaaccat accgttactg ccaacaatag aatgaaatat
     5821 tttctttgca ccatataggg aacggaaacc ttaaattgtt aagttgagaa caaagtattt
     5881 tcatcttttc acatcaacac tcaactgcta atgcctgttt gaatacaaaa tgatgatgtt
     5941 tttcctcttt tcacagaaga ctgtccacta atcacgaatt tccgtcattt tttcaaaact
     6001 tttttcttct ttcacttcat atagggacag caagccccga tttaatgacc tattttcaaa
     6061 aaaagtttag atttcctttt ctcgcaccat atctgtacgc aagtagccat tttgttgcag
     6121 atttaataaa aaatttattt ttcttctttc acttcacata gggaatgaaa acagctcttt
     6181 gctgactaaa tctgaaattt ttcaattaca ttaaaaaata ccgtcattct cttgtggaac
     6241 agcggtatct atacgttctt ctatggttga tttctctaat tgtaataaat gtttatgctt
     6301 gtcttttagt tctgcctgct caatcatatc tttcagtatc gttgtacgat tttctctgtc
     6361 taatgcctgt accattttta tggttggtgc gacttgtctt tgtagccaat gcaacgcttt
     6421 ttgtaaagtg taaggctctg gctttgtggt taagcgtatc ggctctctgt tttcccctac
     6481 aaaccatgcc caatcatcat ttgttatcca ttgacttttt ggcttgtcgt cctctctgtc
     6541 aacaaagcgg acataatgat tgatgataga aaaagcggtg cgttcagcgt cccgataggt
     6601 cagtaaatct acaaccgcat aataggctcg ttcgttcttc atgcgtattt caaaacggtt
     6661 cttaatctcc gtatcttcaa tttctgtgcc attcttgaca tactgctcat aatttttctg
     6721 ataagcacac atatataatt cgctacttgt tgaaccgaga tataaggttt ctcctgtatt
     6781 ctttggtaaa tcgctaccgc atttttctgt accgctgtaa tctttctgca tacggaagta
     6841 ggacatacat tcgccagcct tgtatttttc ctttaacttt ggaatatcca atattcctgc
     6901 tttatcattg atagccaaat caagccgttt catcacacca ccagccgtca tacaatccag
     6961 catgaagtca taccatgagc gttcctgtgc cagcagataa cattccattt gccgacagcc
     7021 tttgcctttc agttccaata aaacacctaa atgctcctgc atggaacaca tgatattgat
     7081 gtcgccaagt acatatttgc tttcatatcc gtattttcca taatcttcat aaagcatgta
     7141 attagatttc agttgtaata catcacggat aatcgccaat gcgtccgtag taggaaagcg
     7201 gacacgaaag taatcaatca ataagaatag tggttcttgc ggattgaaac gctcaatctg
     7261 tttttctatg atttctctta attcgtcatt caatggctgt tttccttttt caatgcggtt
     7321 gtaatattca cggctgatac cacaggcaac agcaagcctt gtttgtgtta ctccgtattc
     7381 ctcacgcttt tgtcttagtt cttcaataaa gtttttatca ttcattcttc aattcctcca
     7441 atcaaaaaag gcaactacca taattgataa ttgcctgtca ttaaaaatct gtatagaaaa
     7501 agcagtcaat cctaagacta actgctttgt gtgtatggat ataaaattgc ttctaatctt
     7561 gaataggtat ccaaatttca ataactgaat tatcactatt ccaatgaaaa cgataatcgt
     7621 atttttcaaa gtgtaggata atatctttta taggaatata cgatgtacta ggaataattt
     7681 cttcataaat cttgccataa gtttcatata ttttatccat agcaccaata tgctccacaa
     7741 ccaaatattt ataagacggt atttctttat acagaaagcc ctctggggta atggttcttt
     7801 ggggaataga acagaaataa taaagttttc catttcttct ttccataaaa gcatatttta
     7861 cccaatttcc tgattgtgaa agatacgctt ttttcaaact actgttaaac tttctccaaa
     7921 actgtgtact aatctgaata ttcttttttt gataattagt cagttctact tcctgcccaa
     7981 taactgcaaa tgcgtttaat tcctttatag aaaaatttat cattttacct tgtgagcagt
     8041 gataacggta taactgtacg catttaccgt tataatacaa ttatcaatcg ttatatacca
     8101 gttttttcct tttcttgtga tttccgcatt agaaaggcta attttttctt tgcaccattc
     8161 aattacattt tctacattca aagataagtt ctttttaatt ctaataacgc caagttcagt
     8221 tgtgtgaagt cttcctaagt tttccagtaa ttcattttcc atattttgat acctcatttt
     8281 tcagcaaatt tatttgtatg ttttataccc caattataac attctcatat cagcactaca
     8341 ataaaaataa ttaagcacca ataatcttat tgaacatctc caaccgtaaa tctttcacac
     8401 catcagtgat aaacactgag ctgacagcaa ttacgacaac taccataatt gcctgcactt
     8461 aaatttctgc aaaagaaaac gacaatcaat ttcttatttt ttgaaatcaa ctgtctttct
     8521 catatttact attcaatttt catagtgata taggtacaat ttatatcact actgtaaaaa
     8581 tggggttttg ccttataaat acagcgtttt ccgcttgtta gtgtatagtg tttcttgttt
     8641 tttgtgcccc ccttgttaga taacgggggc tttgaacggc tcgcaagctc gccgaaccgc
     8701 cgaagcgaac cgcccccttg caagcatggc ggaaaaatct taaggatttt cccgccaacg
     8761 tggtcggatc gctccgtcgg tttaacagct tttctgtttc tctctagtta tctacgcata
     8821 taaatcaaat cggttattcc gttataagtc tgtgttttac aaagccttaa ctttatttcc
     8881 ctgtctatgt tgcccaaaag acgaacccca tttcctaaca gagtaggaat aacagaaatg
     8941 taatatttat caatcatatt tttgcttacc aactgctgaa caagatttgc tccgccacaa
     9001 atccaaatat cctttcctgc ttcttgtttt agccgttcca ataataaaac aggattttca
     9061 cttgtaaaat gaatttgttg ggaagaattg cactctttat gtgttaatac ataagttgta
     9121 aatttattat atacccattc ttgcggagaa agttcagtaa caacctgatg agaagtattc
     9181 caccccatta agacagtatc tatatctttg gtaaattctg tataagtgtc gatgttttca
     9241 ttgtcatttc catgaccatt tagccaagct actccaccat taccgtccgc aatatatccg
     9301 tcaaggctca tggcaataaa taaaactact tttctcattt tttctctttt ataaatacaa
     9361 acaggaattt atttctcttt tttactcata agggaacaac tgattgctac aaataaaccc
     9421 attaaaatcc atggaaatgc tgctcttata aaatcactaa ccatacttta ttcctcctta
     9481 aattccaatt tccttagttc tacaaactga aatttttgcc tatgaatgta tcttgttctt
     9541 tatacgcaaa caggcttctg tgtgcataat ccaatgtctt gaaaacggca tttgaattag
     9601 tccacaaata tctttattac accaataatc aatcagccaa attgcttttt cattgtcgtt
     9661 tactacattc aacgattcta agtatccttt tctttctttc ggtatttttc ttttcaattc
     9721 atcataagat aaccgttcaa gcattttctc agtgctttcc catacttcga aaatatataa
     9781 atataattct tccagattaa gctgttttga aaagtctgca atctgctgtt tcatgagttc
     9841 atttcctgtt gtaatgatag gtgaattgat acgctcttga taattgcctg caaaaaatac
     9901 ttgttcatct tcatttatca ctgtatgcac aactatatct tcaatgcgga aaatatgcca
     9961 aatagaataa gcaatcgtct tactatgata accatcagcg tttatgaagg ggattgagtt
    10021 aaaatcttct ctgcataatt cttctttgaa agatactaag gtttgtatta actgacttcg
    10081 taaagtaagt aaagtatcaa tacccctctt ataagtatct ttctttttta tttgtgcctg
    10141 cattgtttta ttaagttcag accattcctt attcatattc aatcctctct caaattctga
    10201 ttttatcgcc ccatacaaac tggaatttgt cttacaacaa tttatatatg cataaaaagc
    10261 aatatattta tgaaaacacc ctaaatccaa aatttactca attcattttt tccttacata
    10321 aaatgttctc gtatatttca cttcctcacc aagtgtttcc cctatcaaaa cttcaaaatc
    10381 attgatatca aattctggaa agaatgtatc tccatcttca atatttaaat ctacttctgt
    10441 gatatacatt ttatctacta tttgcaaagc ttccttaaat agtccatatc caccagatat
    10501 gtatacatct cgtcctttag ccaataataa tgcatcttct aatgatttaa ctgaaactaa
    10561 attatctcct tgatactctg ttgtggtgga aacaacaata ttcattctat taggcaacgg
    10621 atgaccgatt tcttcataag actttcgccc cataataacc acattacccg ttgttaactc
    10681 tctaaattgc ttttgttctc cctttatttt ccatggtata ttaccattct tgcctataac
    10741 attattcttt gacctcgcaa caatcaaacc tatcatctat ttcatcctcc ataatatgat
    10801 gtcgtacctg tgattccatc aagtgcacca catcttaaca aactcaaaat tatctcgaac
    10861 taaccagctc gacaaattcc ttttttgtct tcatctgcat tatatcatat tgttataagc
    10921 attgccattt cttttctgct tttgaaaagt tacttatcag cctatgtttt gtaaagggtg
    10981 atattcagca tttctcacgc aacaagccct tgacaaacca tagactaata agttttaagg
    11041 taatcaagca gaaatgaaat agtctgctgt ttcacggcaa caaaaaagag ataccaacac
    11101 acttatgtag tatctcttag tctagtagca atattctttt gtttgcaagc aggtaaacat
    11161 gggctgtttc ttaatatctt tttgtttggg aaacaccttt ttcccatcct ttcaccgttg
    11221 ccatctctgc tgcgcacaat gccacaggga taaaccatag caaaccgcct aataacaaga
    11281 aaaatactaa gctgaagcct gaggtcgcga atgtgggata ttcatataat gaaacgacca
    11341 ttgagatggt catagccaaa aagccaaaga acgttaattt ttttgcagga attttcgttt
    11401 tgctttgttg catgaatctt cctcttttct atttgaattt gtttcaattg agtttgttgc
    11461 caaagaagaa aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa
    11521 agtgcatcgc cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga
    11581 tgacaccaat cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc
    11641 tgttagatgc aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac
    11701 cccacacatc aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac
    11761 agacgatacc cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac
    11821 aaggcgtaat ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc
    11881 catttccttt tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga
    11941 atgttgaacc aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa
    12001 aaccgaacca gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat
    12061 gctgacttct ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag
    12121 agccgaatcc agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct
    12181 gatccaaaaa tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc
    12241 aaaaaataac aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca
    12301 tcaaaggaac ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa
    12361 tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg
    12421 ccgggtttcc aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata
    12481 cccacataat cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat
    12541 tattcttccg tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca
    12601 ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt
    12661 ttttcgcctc ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg
    12721 tttttttgaa ttaatacttg catttataaa agacagacaa ttaataaaag cttgatgtgt
    12781 tgtgctcttt ttcgtttttc acacaaataa agacaaaaaa taaacggtct tctttttata
    12841 gaaaaccgtt tattttcgtc tttttttatt tttaaagata ttttttcata aggaataaag
    12901 aatacttcat ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat
    12961 ttgttcttca tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat
    13021 gaaacgacta atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca
    13081 attgtt
//
LOCUS       DACEZN010000069        13055 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEZN010000069 DACEZN010000000
VERSION     DACEZN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.069"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..340
                     /locus_tag="IXH60_001566"
     CDS             <1..340
                     /locus_tag="IXH60_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1990490.1"
                     /translation="PVQAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSA
                     KEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAA
                     KAFWEANGWY"
     gene            complement(664..747)
                     /locus_tag="IXH60_001567"
     tRNA            complement(664..747)
                     /locus_tag="IXH60_001567"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(711..713),aa:Leu,seq:cag)
     gene            1275..1775
                     /locus_tag="IXH60_001568"
     CDS             1275..1775
                     /locus_tag="IXH60_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1990491.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1965..2309)
                     /locus_tag="IXH60_001569"
     CDS             complement(1965..2309)
                     /locus_tag="IXH60_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1990492.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2463..3383)
                     /locus_tag="IXH60_001570"
     CDS             complement(2463..3383)
                     /locus_tag="IXH60_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1990493.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3570..4061
                     /locus_tag="IXH60_001571"
     CDS             3570..4061
                     /locus_tag="IXH60_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1990494.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4085..6460)
                     /locus_tag="IXH60_001572"
     CDS             complement(4085..6460)
                     /locus_tag="IXH60_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1990495.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6786..7412
                     /locus_tag="IXH60_001573"
     CDS             6786..7412
                     /locus_tag="IXH60_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1990496.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7435..8139
                     /locus_tag="IXH60_001574"
     CDS             7435..8139
                     /locus_tag="IXH60_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1990497.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8331..9164
                     /locus_tag="IXH60_001575"
     CDS             8331..9164
                     /locus_tag="IXH60_001575"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1990498.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9361..11289
                     /locus_tag="IXH60_001576"
     CDS             9361..11289
                     /locus_tag="IXH60_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1990499.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11427..11882
                     /locus_tag="IXH60_001577"
     CDS             11427..11882
                     /locus_tag="IXH60_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1990500.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12352..12621
                     /locus_tag="IXH60_001578"
     CDS             12352..12621
                     /locus_tag="IXH60_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990501.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         4003 a         2444 c         2368 g         4240 t
ORIGIN      
        1 acctgttcaa gctgaacaac ctgttgtaca agaaacggtt caaacggaaa cacaagcagc
       61 gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa actgctgcta ctccagcaag
      121 tacaagctct gcaaaagaat ggatcgcaca aaaagaatca agcggttctt acacagcaac
      181 gaacggacgt tacatcggcc gttaccaatt agattcttct tacttaaatg gtgactactc
      241 tgctgcaaac caagaaagag tagcagaaca atatgttact tcacgttatg gttcttggga
      301 agcggcgaaa gcattctggg aagcaaacgg ttggtactaa gattcattat ttgaacgttt
      361 aaacacattc aaaatatagt aagaggtcat gcgatttatt ctttcgcatg acctcttttt
      421 gagtttaagt ttagtcttta ttccttaact atcgtttgcc agtatttctt gtatcagaat
      481 ctataaatct actatttgaa tcaatctatt aggttttaaa ctgtcccacc ttcacacaaa
      541 ttttttttga tttatttgaa tctggttccg ttgtttaaaa tttttcctat attcttttag
      601 ataatatgtt attttctttc tcatcgctcc aggtgtacct tctctttata aaaaaacaaa
      661 ttatgcggct aagaagactc gaacttccac gaggtcaccc tcactagcgc ctgaagctag
      721 cgcgtctgcc aattccgcca tagccgcatc acatctttta gtttaggatt acatgtgttt
      781 tttgtcaaga acatattttt gttctttttc atttctgtac gccctccctt atgccatcta
      841 cacaaataat tcatccgagc aactccatca tctattgagg gttggtgaaa aaatctgttt
      901 tatccatatg atcgatttca atgttaaacg aacgactctg ggattagtaa ctattttatt
      961 tttttcttca tttctttttt cctctttttc tcgattcagc tatcttcttt catttggata
     1021 ttgattattt cttctgttga ttactatcct tctggaattc tagacgctga agtaattcac
     1081 gttttctttc ttctagagat cataagtaac gaatgaacga acttcaaata atttttttat
     1141 tgtaattcat atttgtattg ttcgatgatt cttaatacga tatgacacaa taaataaaaa
     1201 taggcatggc tatttacaac aatagtgcta tagtagaaaa caaatgaata aggttactta
     1261 aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc agatttaaag aaagcttatc
     1321 agctacaaac tagctttgga ccaaatgaga gtggatttat caacaatgct tatggctata
     1381 catttgctga atttttagat tatgtaaaaa aatgcgaaaa aacgtcttta ggaatcgatc
     1441 ttcctgagag ttacgttccc gcaaccgtct ttattttaga aagtgatgaa catgagtatg
     1501 taggaatttt taatttgcgc cataaactga acgaaacatt aagaaacggc gctggccata
     1561 ttggttacgg catctcccct aattatcgta aaaaaggata tgccacacaa ggattagcat
     1621 tagtactaca agaggcaaaa aaaatcggca ttgatgaggc acttttgtct gtaaataaaa
     1681 aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc tgcgatttat cgtgaagatg
     1741 agaaagaata ctatacgaaa atcaaactga tgtaaaaaag aaggattcca aaaggaaagg
     1801 gatttgaatg tcctttatca atagaacaca tttatttaat taacttataa tatagcagac
     1861 aaaattttat aaacaaagaa accttttatc tcttttcttc tagaaaggat aaaaggcttt
     1921 tttgtttttc ctatttgctt ctcatagcta gtcagccaag atttctatcg tatccccaac
     1981 gcaatccgtg cataacggct catcttatct gtcgtccatg ctggatacca gactagtttg
     2041 acttctgctt tcgttacttc tggcacttct tttaatgcgt catggataga ttctgttaaa
     2101 atatctgcta atggacagcc catagttgtt agagtcatct taatgactgt ttctccattt
     2161 tcaggattga attctatatc ataaatcaag cctaaattga cgatgtcgat tcctaattca
     2221 ggatcaatca ccatttccaa ggcagcaaga atacgttctt tgattagttc tatttcttgt
     2281 ccagaccgtt cttggtttgc ttcgctcatc gtacgacact ccttaacacg atttttattt
     2341 atcatactct ttttttagct gcttgtaaat gagaatttag aagtaagatt ctcgtttaaa
     2401 tttataaaaa tgttcttatt agatgtaata gattaaattt attctaaaat aaacctgatt
     2461 cctcattttt tttgtgaacg attttttatt tctggcaata cgccagttgt tcgatacatt
     2521 ttttgttcgt tttcatttgc aaaccggcca aatactgaat gagttggtaa aatattgata
     2581 aatgcatcgt cgtcgatttc atgaacgatc tgatcaagat catacatttc gtagcgggtg
     2641 atgaccatca tgatcatgtt cccttgtttt tttgaatagc caccgacaga cggcagtaat
     2701 gtcattccac gtaccatccg ttttgctagt tcttctgtta cctcttctgg cttgacggta
     2761 acgatcattg ctgttacttt ttgatgactc gtgtggatgg catcaattac ttgagtcatt
     2821 gcataaatgg aaataatcgt atacaaggca ctttcccagt taaagaagaa gcctgcgatg
     2881 actacaatga taccatttag aatcagcatg tagtttccga ctgtttttcc agttgtttgt
     2941 gaaagtacaa gagagatgat atccatccca ccagttgtaa agcccatttt taaagataat
     3001 cctacgccaa gacctaataa aacaccgccc actagggcat tcatcaaaat attatcagtg
     3061 atctgtccga ctggaagcag catcgtcatc accgatacac atacaacatt cacaaaacta
     3121 aggatcgttg ctcgttttcc tactttgaga aaccctaata caaacacagg gatattcaaa
     3181 agaaaaataa atgttcctgt gtctatcttg atatgaaacc attcatataa aagcgaggaa
     3241 atgatttgag cgatcccatt catccctgca gaaaagacat tggctggaat caaaaaataa
     3301 tttaaagcga ccgcagcggt caatccggtt atcaaaataa ccactatttt ctttaatctt
     3361 tcatttttat gatacgcagt cataatttta ctcacaagca cctctcccct actttggtat
     3421 tccaaatgta agaataacag tttatctatt aaaataaaat acttcgaact caaatgagaa
     3481 gaattttagc aaacaggtaa gaatctgccc taatcctatg ataagataga tgtatggtaa
     3541 atatttatat gtatatcagg agggttccta tggcaaaaga tgctagcttc gacattgtat
     3601 cagaagtcaa tacagaagaa gtaaaaaatg cgattcagca aacaacaaaa gaattgtcca
     3661 atcgctttga cttcaaggat tctacagtag aaatcaaatt agagaacagc aatctagttg
     3721 tattgagcga tgatgatttc aaaatcgaac agatcaaaga tgtattattt ggtaagctga
     3781 tcaaaagaaa tgtgccaatc aaaaatatcc atttttccga atcagaacac gctctaggtg
     3841 gcaaagctcg acaaactgcc gatttggtca acggaatcga tcgtgaaaac gcgaagaaaa
     3901 tcactacagc tatcaaaaat gaaaaactga aagtaaaagc acaaatccaa gaagatcaga
     3961 ttagagtgac aggtaagaac cgtgatgatc tacaagctgt gattgcttta ttacgtaaac
     4021 ttgacttacc aatcgagttg caattcacta actatcgtta atcaaaaaac gaggctgacg
     4081 tctgtcattc ctcgtttttt tgatttactg cttcttttga taaccaaaaa cgctgtattc
     4141 cttcttccaa ttccccaaca cttcgtactt ctcttgctgt ttgccaatgt tgtgggaata
     4201 aacgtcgata agaaaaacta gaaaatcgtt gatctgccaa tacaacgatt ccttgatccg
     4261 ccatgccacg aatcactcga ccaccagctt gcaagacttt gttcattcca ggaagctgat
     4321 aagcgtaagc aaatccaagg tgttccttct catcataata tgatttgatc agttcctgct
     4381 catgattcat ctgcggcaaa cctacaccta cgatcatact gccgatcaat cggtcccctc
     4441 tcaaatcgat tccttctgaa aaaatcccgc caagtacgca aaatccaatc aatgtttcag
     4501 cgggatttgt ttgaaatttt gttaaaaatc gttcgcgttc ttcctcattc atgtccgttt
     4561 cttggattag cacattgctg tcgggatatt gctgtctgaa tgcttccacc gcaagatcta
     4621 aatattgata agaaggaaag aaaacgaaat aattcccgac ctttccatca gcaaaagcat
     4681 gaatcgtctc tactaatttt gccagactgt gttccctttt tcgataagtc gtctctaggt
     4741 aatttgcgat gaaaacttgc tggtttcctt ctgggaaagg actcggcagt ttgtaagcca
     4801 gacttttttg tccccccagt gtttcctgat aataagagag tggtgaaaaa ctagcagaaa
     4861 aaagaatact gctttttcct ttatccaacg actgctcaag aaacaagctt ggatcaatac
     4921 aaaactcttt tactatcaga tcgcgatacg ttttttcgac tgttgtttcg tagtgatcat
     4981 cataaaattc actgaccttc aaaaaatgaa gcaaatcaaa ataataagga agcaattgtt
     5041 cttgtgatgg gtgctcagga aattctgcca gccattcttt aattttttcg cttaattgat
     5101 aacctgcctt tactaaagat tcagccggtg ctttttgatg atgataatcc caatgatctt
     5161 cttttgctat ttctcggatc gaatcaaatt cttttaatag tttgttgaaa cggcgatgaa
     5221 gttttttgaa ttcctttggt atagcttcct tacttcgttt cgttttttca taagatagtt
     5281 ctgccgaata catctctcgg gaacgattca ccaaattatg tgcttcgtca atcaaaaaca
     5341 gatagtcctc gtttttttca tcttcaaaaa aacgtcttaa ataaacagtt ggatcaaata
     5401 aataattgta atccccaatg attacgtcac accagatact gacatctaat gatagttcga
     5461 aaggacaaag tgtatgcttt cttgcataag tttcgatgac ttcacgagtg atctgattct
     5521 catgattcaa aagatcccat aaaccttcat taatccgatt gtaataacca ttggcatatg
     5581 ggcactgatc tggattacag gtcgtctcat ctaaaaagca gatcttgtct ttcgccgtca
     5641 aagtgacact ttttacttca gctcctgtat cttttaatgc tgtcatagca tcttcagcta
     5701 cttgtctagt tattgtttta gccgttagat aaaagatctt ttctccctct tcttccccta
     5761 cagctttcaa agcggggaat aacgtagaga ttgtttttcc tgttccggta ggtgcttcta
     5821 caaacaattt ctgtttcgta cggatcgttt tataagcagc taccgctagt tcacgctgac
     5881 ctttcctata ctcatcatgg gggaatttca aagccatcaa agaggcattg cgtacagtgc
     5941 gccagttttc ttgaaaaaca agccattctt tataatcttc agtcagcttt ttaaaaaaag
     6001 tttctaactg ctctcttgtc tgatgttcaa cctttctcgt gatcacttct tctgccgtct
     6061 ggaagtaagt caattgcaga tttatttctt gtaaatcatg ttcttgacta tagatatagg
     6121 catacaccct tgcttgatga aagaaaagtt ccacttgttc tggttctaaa tcttcaaatc
     6181 gtggttcgga ggttttgatt tcatctatat aataaatacc gtctcttttg aaaataccgt
     6241 ctgcacggcc ttcaacgatc aggtaatatt catctaattg aactgttgtt tttaaaaaga
     6301 cttctttttc atagttttct cccgcatctt tttggagctt gcggtggatt ctcgctcctt
     6361 ctaatgcagt atggttactt tttttacgat tgtcgatact tccacgtcgc aaaataaatt
     6421 ctactaagtg ccgcacagat atttttgact gtttgatcat ttccaccacc tctttctctc
     6481 taagattacc tgaaaaaacc tagtttagca aaaagagctc tctttccaaa cataaaaaaa
     6541 caccctgctg attaggcagg gatataggat atagaatttg gatttttgga tgaatctaat
     6601 gtaacaagga catattaaaa tgctatgaaa acttactttc cttctcttta gaatatccaa
     6661 atacttttca tatagtctga taattcagat aagtaaagag aaataagtac ctgtttttgt
     6721 ttcctagcaa tgaatgcgtt atactaatgg atgaaagaag ttttggctat caaggaggaa
     6781 tgcacatgca tatcgaaaga ttaaaaacag gaatgattga agaaaactgt tatttggtct
     6841 acaacgaaga agcacttttg atcattgacc ctggagaaga tgcggaacga atcaaacaag
     6901 aaatcaaaaa aacaaaccaa cagccggtag cgattttgct tacccatact cactacgatc
     6961 atatcggtgc agtggaacct ttacgcgcgt attatcaaat cccagtttat gtcagcccac
     7021 tagagcaaaa atggctaggt gatccgattt tgaatctttc tggactcggt agacatgatg
     7081 atattgctga tgttattgtc tcgcctgcag aatatgaatt tgaaatgaaa acttatactt
     7141 taggaaacat gtcttttcgc gtcgtcccta ccccagggca ttctatagga agcgtcagct
     7201 ttatttttga tgattttgtc gtttctggcg acgccctgtt tagaggaagt atcggtagaa
     7261 ccgatctgta tacagggaat ttagaacaat tacttcatag tattcggacc cagctgtttg
     7321 tattacctga tgaatttgca gtctatccag gacatggcga tgcaacgacg attgaacagg
     7381 aaaaaaggac aaatcctttt ttcaatcgtt aaaacgactt aggaggagaa taaaatgaca
     7441 gaaactacac tttatatcgt acgacacgga aaaacgatgt tcaatacaat tgaacgagta
     7501 caaggctggt gtgatacacc cctcacaaag caaggacaag aaggcattca ttatttaggt
     7561 cgcgggttaa aaaatgtcga cttttcattt gcttattcta gtgacagtgg tcgtgcaatc
     7621 gaaactgccc gtattattct aggagaacat tctaagggaa aagaaattcc ttattttatt
     7681 gaaaaaagga ttagagaatg gtgtttcggg tctcttgaag gtggatatga catggaaatg
     7741 tggggtgtga tccctcgtgt attgaatttt aaaacatatg atgaaatgtt taccacagaa
     7801 gtttcttttg aacaaattgc taatgctgta tacaacttag ataccgctgg gtgggcggaa
     7861 acttatgaaa cattgcgaga ccgtgtatgg acaggatttg aagatatcgc ccatcactgc
     7921 gaaaaaaatg gtggtggaaa tgttttagtc gtatctcatg gtttgacgat cgcctttttg
     7981 cttaatctga tcgatcctaa acagcctgtt cgtgctgggc ttttaaatgg cagcgtaaca
     8041 aaggtcactt atgcaaacgg tcgattttct atccacggca tcaatgatac gtcttatatc
     8101 gataacggga aaacacatag tcgcgactcg tttctataat ggggatattt taatttttac
     8161 cagagaatca taaaaggcaa agtctgaacg agggctttgt cttctttttt ctcttcgtgt
     8221 acttatctgc tttatttctc aagtttttct taatattaaa aaacagattg ggtttctcaa
     8281 aaaataacgg tatactataa gatgtatctt aattgggaag gaaggaattt atgttttttg
     8341 caaatattat caaagcagtg attctcggta ttattgaagg aataacggaa tggctgccca
     8401 tcagtagtac tggtcatttg atcctagcag atgaattcat caaactggat gccagttcac
     8461 aatttatgtc gatgttcaat gtcgttatcc aattaggggc gatcttggct gtcgtcgttc
     8521 tttattttca caagttaaac ccattttctc caagtaaatc aacggtagaa aaaaaagata
     8581 cgtggacact ttggtttaaa gtgatcgttg cttgcatacc agccatcatc attggtttgc
     8641 ctttggatga ttggttagaa gcaaatttcc acaagttctt accagttgcc attgttttga
     8701 tcgtttacgg tattgccttt attattgtcg aaaaacgcaa caaaaacaaa acacctcgtt
     8761 ggacttctct gaatgacttt acgtaccaag cagctcttct tgttgggtta ttccaagtct
     8821 tggcacttgt tcccggaaca tcccgttctg gcgcaacgat cctaggatct atcttgattg
     8881 gtgcttcgcg ttttgtcgct acagagtttt catttttctt agggattcct gtcatgtttg
     8941 gcgcaagcgg cttgaagatc gtcaaatatt tagctgaagg gaattcattc aaaatggaag
     9001 aaactgttgt tcttcttgta ggaacattcg tatcttttgt ggtttccgtc ttagctatca
     9061 aattcttact aagctatttg aagaaaaacg actttaccgc ttttggctgg tatcgtatta
     9121 tccttggtat cattttgatt ggttattggt ttattgcaat gtagaaaaaa ccagaacact
     9181 tctcttattt gagaaatgtt ctggtttttt attttcttta ataataatca ttataaataa
     9241 taaaaacaac atcaagctta atcaccaaat gataaccatt atcgttctca tttagctgtt
     9301 attattatca attacatttt tattatgcta tccttgtttt gacaaggagg tctaataacg
     9361 atgactaatc agaaaaaagc ttttgcagca acaatcggct gccttttatt tatgatcttc
     9421 ggttttatct tcgaccgatt ctctcttacc atcaccccac ttttttatat cagtgccatt
     9481 atcatcggtg gatataaaca aacatcggaa ggcttgaaag aattgtggaa tgatcggaca
     9541 ctgaatgtgg atttattgat ggctttagct gctattggtg cctgtttgat cggtaattac
     9601 tttgaaggag caatgttgac attcatcttt tgtttaagcg gtgcattaga ggaatacaca
     9661 actaataaaa gtcaaaaaga aattactgct ttgatgaatc ttcaaccaca aaaagcccaa
     9721 ttattaaaag aaaatggaca aatgcaagaa gtagatgttt ctcaactaaa ggtggataac
     9781 ttggtatttg tcgctaaagg agcagctgtt ccgatagatg gctttttaga aagcacacag
     9841 gcaacgatcg atgagtctgc attaagcggt gaatctgtgc ctgtagaaaa atatttaggc
     9901 gatgagatgt atgcaggaac gctgaatcaa ggaaatccca tcacgattcg agtaacaaaa
     9961 acgagttccg aaactgtctt tgctaagatt atccaattag tagaagaagc gcagaatacg
    10021 ccaactcaaa cagcttcatt tatcgaacga atcgaaaata attatgtaaa attgattttg
    10081 cttgctgttc ctctaatgat ccttctcccc cactttttcc taggttggag ttgggatgaa
    10141 agcttttatc gaggaatggt attattagtt gtggcttctc cttgtgcgct tgtcgcatct
    10201 gctacaccag cttctctagc tgctctttca aattctgcaa aaaatgggat tttaatcaaa
    10261 ggcggtatcc atttagaaaa gctttctgaa ttaaaagcga ttgcctttga taaaacagga
    10321 acgctcacta aaggaaaacc agttgtaact gacagctttt tctttgatga aaaggatttg
    10381 gcacaacaac ttcttgttgc tatggaacaa aaaacgacgc atccacttgc tcatgcgatt
    10441 ttgcaatatt tcgattgcac aatacctgaa gaaatccaat acttaccaat agaagaaatt
    10501 actggttttg gattacaaac tacctattta ggtgcgcaat ggaaagttgg caagcacgca
    10561 tatgatcctg agacgatgat aatcagcaaa gagattgcgg aaatgatcga acgtttggaa
    10621 aatcaaggca aaaccgtgat ttatctttct aaagatcagc agttaattgc tgttttaggt
    10681 ctgctagata ttccaaaagc aaatacccaa caagtaattt cctactttaa atcccaaaac
    10741 atccatacaa gtatgataac cggcgaccat tctggcacag caaaggccat tgctgaacaa
    10801 gtagggattg aacattacta tgcaagctgt actccggaag aaaaaacaca actagtcaaa
    10861 aaagagaatg aactttacca agtaaatgcg atggttggtg atggagtcaa tgatgcgcct
    10921 gctttagcag ccgcttcttt aggtgtcgct atggggcaag ggacagatgc cgctatggat
    10981 atcgcagaca tcgtcttaat gaaaagcgac ttaaataaat ttgtctacag tcatcggcta
    11041 tctttaaaaa tgcggcggat catcaaacaa aatattattt tttcgattct tgttattctc
    11101 ttgttgattg cttctaactt cctgcaattt ttaaatctac ctcttggagt tgtaggacac
    11161 gaaggaagca caatattggt tattctgaat ggcttgcgtt tactaagacc tttacctgat
    11221 ctttctgatt cttcaaaaaa atgtaagtct tgtccactat acaaagcgcg gctgtcttct
    11281 gtcaaataag caaagtgttt ttttatgatt ttgtttattt tatgaaaaaa acagcttatg
    11341 aataggtctt gaaggatgaa ttcctacttt tgtaactttc taaaaacatg ttatgattgt
    11401 ttcgaagaaa gtgaggaata tatttcatgg aaagttggct ttttttagga attattttac
    11461 ttgtagcaat tttagctaaa aatcaaagtc tgatgatagc ggcagctttt gttttgatta
    11521 tgaagctcat accagcctcc cgttcctttt tcccttggct tcaaggcaaa ggaatcaata
    11581 ttggggtgac gatcatcaca gcggctattc taatccctat cgcaacagga gaaatcggtc
    11641 tacgtgatct attagaagcc tttaaatccc ctattggctg ggtagctgtt ttttgcggtg
    11701 ggctagtggc tgtcttatct tccaagggtg taggattgat tgcttctgat ccacaaatca
    11761 ctgttgcact tgttttcggc acgattttag gtgttgtctt cttaagagga atcgccgccg
    11821 gaccggtcat tgcttctggt atcacttact gtatcttaca agtatttgct ttaggaaaat
    11881 agaacaaatt ttgtctattt taggagattc ttaacacccc caatttactg atccgtgata
    11941 tactgacaac gaggtgatac aatgttcgaa aaattaaagt atttcatagg aacaatcggc
    12001 tggtttcttc tagcggtact tttgattctg ggcttagctt atctattatt tcctgatttg
    12061 atacaaaatg tgtttgatac tgctttttga ttgacctatt aaaacgatat aggggatgct
    12121 cctgcgatgc aggggctttt tttaatcaat gttttgtaat aatttaggcc gtgccaatga
    12181 gacttcctat agaattcgat gctctttttt ctttttggct cttctgctct tttttgttat
    12241 actagattac agtggacaaa agaaagaatg atcgcatcca cttcaacgaa tatcaaaatg
    12301 gaagaattag tgcctgtttt caggttactt tgaaaagaaa ggaagacgct tgtggaaact
    12361 tttgaaggtc ttttaattga tacgtacgtt ttacaagaac agccaatgat gctgactggg
    12421 atcaacttgc agcttgagga ccgtgtgatc aatggcctgc ttaaagatcg ctatttggca
    12481 aaatatatct tacgtttgcc cctaaataaa tatgtgctgg aagtaagtgg caaatggaac
    12541 cgtcggaacc agttggtcat aaaaaaaatg ttgataaaaa atatcgatga atacattaaa
    12601 ataatcggta caccagaata gcctttactg atcaaaggat actggttata aaataactgc
    12661 agtttcttaa tagaaaaaca aagaacaaca gggaatcgta tcccttttca aaaaaaactt
    12721 tgcaaacggt tctctttgtt atataataac ttagtaccaa acattcccat tcaaactttg
    12781 tttaaataga taaataaaaa tctagtttgg tttgacgcta ttgcctccca caatagcgtc
    12841 tttttgattt ttttcagctt gtactatccg tttttactga aaaaaaatcc taataaaaga
    12901 ctgttaagga aaaaaacttg tcatgttcat attgcaaagc acgctgcctt taggtataat
    12961 aatgaatgtt gaattatgcg gtaacacgcg taagaactta agttatcgga ggtgaaacta
    13021 atgccaaaca ttgaatctgc aatcaaacgt gttcg
//
LOCUS       DACEZN010000070        12970 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEZN010000070 DACEZN010000000
VERSION     DACEZN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.070"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..215
                     /locus_tag="IXH60_001579"
     CDS             <1..215
                     /locus_tag="IXH60_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1990502.1"
                     /translation="NWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPIE
                     NGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(370..1338)
                     /locus_tag="IXH60_001580"
     CDS             complement(370..1338)
                     /locus_tag="IXH60_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1990503.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1570..2403
                     /locus_tag="IXH60_001581"
     CDS             1570..2403
                     /locus_tag="IXH60_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1990504.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2437..3276
                     /locus_tag="IXH60_001582"
     CDS             2437..3276
                     /locus_tag="IXH60_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1990505.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3302..3862
                     /locus_tag="IXH60_001583"
     CDS             3302..3862
                     /locus_tag="IXH60_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1990506.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            4061..5782
                     /locus_tag="IXH60_001584"
     CDS             4061..5782
                     /locus_tag="IXH60_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1990507.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5947..7089
                     /locus_tag="IXH60_001585"
     CDS             5947..7089
                     /locus_tag="IXH60_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1990508.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            7105..8316
                     /gene="thiI"
                     /locus_tag="IXH60_001586"
     CDS             7105..8316
                     /gene="thiI"
                     /locus_tag="IXH60_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1990509.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8889..9536
                     /locus_tag="IXH60_001587"
     CDS             8889..9536
                     /locus_tag="IXH60_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1990510.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            9929..12574
                     /locus_tag="IXH60_001588"
     CDS             9929..12574
                     /locus_tag="IXH60_001588"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1990511.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
BASE COUNT         4403 a         2223 c         2740 g         3604 t
ORIGIN      
        1 ttaattggat cattgacgta ttaggatatg actttttgac tacgcatgtt ccagaaaaag
       61 taattattcg atttgataaa gaagtagagt acgggaatga gatcgaaagc cattttgaac
      121 aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat ccgaatgcaa gaacagctgt
      181 attgtgaagc aaatattata tggaaaaata aataaaaaag aggttgtgac agaagggggc
      241 aacttcaaga aataagaaga aatttacgaa aattgtttct caaatttttg taaattttcg
      301 acttagtttt cgaagaagct atttttgtca cagcctctct ttttaagttt agctattaga
      361 taatgatgtt tagttttgga taaacgaaat caattcttca cccgacaaac catagtttac
      421 agcaatcaat agttttaaac gtgctttttg actattaata ctgttacaga aaataatccc
      481 cattttttct agttcttttc ccccgccttc ataatcataa acaggctctg cgattccatt
      541 gaaacaacga gaaacaagta ctacagggat tttttggaca agaagctttt ccaaagcagc
      601 taaggcttgt ggagggagat tccccgcacc taaagcttcg attaccaaac cgtctacttt
      661 cgtatgggcg atggcttcca ataagtcccc ttgcatccca gcatatgctt taacgatcgg
      721 gatagtccca tctacagcgg aaatgtccag tcgctttgtt tctaaaagtt cctgcaagaa
      781 taaaattcga ttcttagtta ctaagccgat gggacccaat gtaggtgttc ggaaagtagc
      841 aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa tggatctcat cgttcatgac
      901 caccaaaacc cccttatcta gcgcttcttc acaacttgcg acacgaatcg cactttcaaa
      961 attataaagg ccgtcacttc caagttcatt acttgaacgc attgccccag tcaaaacaat
     1021 tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca gtttcttcta atgtatctgt
     1081 accgtgtgtg atcaccactc cggatatacc tgatgcataa gcggacaaga tacgttcttt
     1141 taataccaac atgtgagaag gcatgatgtg tggtgaggga agattgaaga tatcttcaac
     1201 taataattct acatgcttgg ggattgtgat ttctgcatct aataatgggt ttgccacatc
     1261 aggactaaca ccgcccgtga gcatgttttc tttcatggcg atcgttccgc cggtgtgtag
     1321 tacaagtatt tttttcattt tacagacttc cttacgaata attttgcata gccgttaaat
     1381 cttattgtat actatataaa agagttttgc tatcagtgaa gaataacgaa tagcctaact
     1441 tatcataaaa aagctgacct tattaaaatg gaaaagactc aaccttgacc tcctaataca
     1501 atggagtatc tggttgagtt tcttttacaa gaaagcagcc aaggaaatca atcacgaagg
     1561 tggccggaaa tgatcagaat cggattaaca tcgtttaaag aacatgacaa attaactggt
     1621 aaaaagaata acactcttta tgaatatgca gggtttctac ccttagtgga attagatact
     1681 gcctattatg gaattccccg aaagtcctca gttgagaatt ggataagtga agtacctgaa
     1741 tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg gtcaaggaga ctggaagcct
     1801 tattatcaga ccgaagaaga gatgattgcg cattttttag atgttatgtc gccaatggta
     1861 gatgctggaa agatgttttg ctttttagtc caattcccct catcattcaa atgtacgaaa
     1921 gaaaatgtcc agtacttgcg caaacttaga aagtggtttg agggttatcc tgtttctata
     1981 gaattgcggg attattcttg gtacagcgat gaatttcttg aacgtacaag aaactttatg
     2041 tctagagccg ctttttcttt agccatcatc gatgagccac agttgctgaa tacaacgatt
     2101 ccttttgatc catttgtcac caatccgaac tttgctcttt ttcgattcca tggtaggaat
     2161 gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa aaagaacgtt atatcgttat
     2221 tcagaaaaag aactgcaaga actagcacat gaagtcaaag tagttgattc taagacaaaa
     2281 gaggtaggtg tgatttttaa taataattct ggtggtgatg cggctgaaaa tgcattagca
     2341 ctgcaaaaaa tattaaatat caagccagat ggactcaatc caacccaaat ggatctgttt
     2401 taagggctta ttttattcaa gaaaggggcg aagtctatga tcaaagctat tttttttgat
     2461 attgacggaa cattggtcac caatcgttca aaagcgttgg aatcaacaaa acaagcaatt
     2521 gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa cggggcgcag cccagtaaag
     2581 atcaaagaaa tcattgatga attggaatta gatatgtatg tcgtgtataa tggtcaactg
     2641 gtttttactg cagatcgcac gattatcgac cacccttttg aacaaaaagt attggaacat
     2701 attgttgaat ttgcagatga gaaccatcga caaatcgttt ttggtgcgag aaatcggtta
     2761 gatggatcaa cgacgatgct attaggacaa tcgattttta tcaagcgact agtcagcttt
     2821 ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc tccaggtttt tagccctcac
     2881 cgacaaaagg atcgttatga aacgttagag atattaaaag aaccaattta tcaatgtata
     2941 ttgctcagtc cggagtcaga acagaaaaag ttagaacatc gctttcccga gtgtacgttt
     3001 cagcgttcaa atacgtatac tgtagatatc atcccaaaag gtggttcaaa attactaggc
     3061 atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag aaattatggc attcggcgat
     3121 cattacaatg atatcgagat gctgaaaggt gtaggaattg gagtggccat ggggaatgct
     3181 cagatcgaag taaaacatgc tgcagacttt gtgactcaat caaatgaaga agatgggata
     3241 tacgaaggac tgaaacactt cggtctgatt tattagtcaa tgaaagaagg aaaaaatcag
     3301 tatggaagca gagacacctt ttcaaaaagt agaaacattc cacaaaactt ttgatccaag
     3361 aagaccagag atacccacgg cttttacaga aaatgaagct catacacgtg ctgcgttcaa
     3421 aatagaagaa cttgttgaat ttgttgcagc tacttcacct aataaagaac aactgcatga
     3481 agctgttatg ctgctgcacg aagcattaga taaagcagaa gaaaaaatac ttggtaaaac
     3541 acagtgggga agtgatccgc tggttgaaca agtagatgca ctgacagata tcctttactt
     3601 tacttatggt tctttttcat tactaggaat agtgcctgat cgaatattcg aaatcgtcca
     3661 tcaggcaaat atggggaaat tatttccaga tggaaaacct cattatgacc ctgtaacaaa
     3721 taaagtgttg aaacctgaaa actggcagat ggaatatgct ccagaaccta aaatcgcggc
     3781 agaaatccaa cgtcagattc aagaagcgat tgctaagaag aaatccgctg atataggcat
     3841 gtgcacaaat gaaaaagagt gatcagagtt tcactatttt tgttatcttt atacgatgga
     3901 aaaacaaaag attttcgttt aagttagtga atgcatgaaa atagtatgct ataatctaga
     3961 atagatgtct tttgcacgag agacgtatta tttggattta tgccaagcag aacgccatct
     4021 gttgcataaa agatgcatac tgaaaacgga ggaaccataa atgggaaata atgttatcat
     4081 cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc tttttggtta gttacttgat
     4141 gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa cgcaaggaag aattatttga
     4201 cttgccagtg attgaagaag tcgatgatgt taaaaaaatg catctggttg ggcaaagcca
     4261 aaataccttt cgtgaatgga gtcaaaaatg gaatgacata tctacttcct cgttcgctga
     4321 actagaaagt caaattttcg aagtggaaaa cctcaatgaa agttttcggt tcttaaaagg
     4381 aaaaaaagca gttgttgaag cagaagctac aatggaccaa atggagaaac aagtgacgga
     4441 aatccgtgaa ggtctaaagg aattgcggga aacggaagaa cgcaactcac tagctgttca
     4501 gcaggcactt gacgtctatg aagaactgaa agaaaaagtc agagaagacg agcatgaata
     4561 cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg atcgaaaaag aatttacgca
     4621 gtttgtgact ttaaacactt ctggagaccc tgttgaagcc cgcgaggttt tggataaagc
     4681 agaaaaacat acgtatgaag tggaagctct gatgaaaaaa attccaccgc tgtatgagga
     4741 gctccatacc actttccctg aacaacttga agaaatcagt gatacttacg agaaattgat
     4801 ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa gatatcgcaa aagtaagtcg
     4861 aagaattgaa aattcattag ccaatcttga aaaaacagaa gttgaaacgg tcgaatttga
     4921 aaaccgtgaa acagctgatt tgattgattc gttatatgat atcttagaac gggaaatgga
     4981 agctcagaga tatgtaaaaa ctaaccaatc aacgattgca gagtatatca aacatacaac
     5041 aaagaataac cgtcaattat tgattgagct ggatcacaca tctcaaagct atacattgaa
     5101 ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa gtggaagaaa tggaacgtca
     5161 aaatgaacag atgattccac agatccgtca acacgaaatt ccttattcag aagtgcgcac
     5221 gttctataaa acagtcttca aagtcttaga agatatcgaa aagcaacaag tagagatcga
     5281 cgactcatta catgaattga gaaaaggcga aaaagaagcg caagaaaaga tcgatacatt
     5341 cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa caacgtttgc ctggcttgcc
     5401 gaatgattac ctagaattct tttttgttgc tactgaccgt attgaagaac taagtgttgt
     5461 cttgaataaa attcgagtaa acatggaaga ggtcaatcgt ttagttgctc tatgtgaaga
     5521 agatctagaa ttattagata agaagacaca tgatttagta gatgcagcag cattaacgga
     5581 gcaaatgctc caatatgcaa atcgttatcg tcatacacac gaagaagtga gagaagcaat
     5641 tgataaaagc tactatttat tcaataaaga atatcactat caagaagcat tagacgaaat
     5701 cggtacagca ttagagcgag tagaacctgg tgctttcaaa cgcatcgaag atttttattt
     5761 taatcatcca gacttagtat aaagcaaaaa agttagtaaa ccagcaagag caagacggat
     5821 tattgtcttg ttcttgtctt tttttgtata tttgaaaatt gaaacaagtg aaaatttcac
     5881 ctgctcttga aagcaccttg gacaaccgtt ataatagaaa agaatttcaa aaaagagggg
     5941 tttcttatga tatattttga taacagtgca acaaccccaa tctttccgca aagcttagat
     6001 gcatatgtaa aaaccagtca gcggattatt gggaatccat caagcttgca tgatttaggc
     6061 aatcaagcta accggttgtt gcagcaagca agaaagcaga tcgctgattt gatccatgta
     6121 gaaccagaag aaatctattt tacttcaggc ggaacagaag gagataactg ggtattaaaa
     6181 ggaacgatga tcgaaaaaag agaatatggg aatcatatga tcatttcagg agtcgaacat
     6241 ccggccgtca gcgagacagc agaacaatta aaagaattag gaatcgaagt aagtatcgcc
     6301 ccagtggata aacgcggatt tgtacgtgtc gacgaattga aagaattaat aagaaaagag
     6361 acagtattag tctctgttat ggctgtgaat aatgaagtag gatcgattca gcccatccag
     6421 gaaatcagtg atcttttgga tgattttcct aaaattcatt tccatgtaga tgctgttcag
     6481 gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa gagttgattt tgcaacattt
     6541 tcagctcata aattccatgg cccacgggga actggtttca tttattggaa aaagggtcgt
     6601 cgtttagcac cattactgac aggtggaggg caagaaagaa atcagcgaag tgggacagaa
     6661 aatgttccag gtattgtggc tatggttaaa gctttacgtt tatcattaga gataaaggaa
     6721 caaaaacctg agcagacaca acgcttaaaa gtagctctaa tcaatgcgtt gaagacgtat
     6781 gagaaagtga cgattttttc agaaggaact gactatgcac cgcatatttt atgttttgct
     6841 ctaaaaggaa ttcgcggcga agttctcgtc catgccttag aagaaaaaca aatatttgtt
     6901 tctactacta gtgcctgctc ctcacgtaaa catatggcaa gcagtacatt gcacgcaatg
     6961 catgtaccaa atgatctggc tacatctgct gttcgaatca gcttagatga aaacaattcg
     7021 cttgcagaag tcgagcaatt tatgattatt ttcaagcagc tttataagaa attttctgca
     7081 atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa ttatggtccg ttatggggaa
     7141 ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc aactagcaca aaatgtcaga
     7201 caagctttga aagactttcc agaaatcaaa atccatgctg atcgggaccg gatgcatctt
     7261 ttattgaatg gcgcagacgg gcagctagtt attccaaaac tagcaaaaat atttgggatc
     7321 caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg aagttttgaa acaagctgtt
     7381 caagacatca tgaaagaaat ttatactggg aaagaaacat ttaaaattat cgctaaaaga
     7441 agcgatcacc aatttgaatt gacaagtaat gaattgaatc aaacattagg taatgctgtt
     7501 tttgatattt tcccgcatat ccaagttcaa atgcgtcaac cagatattcc attacgtgta
     7561 gagattcggc gtgatggcgc ttatctatcg tatgaaacga tcaagggagc tggaggattg
     7621 cccgtaggaa caagcggaaa aggaatgctt atgctttctg gggggattga ttctcctgta
     7681 gcagggtact ttgccatgaa gcggggagtg gaaattgaag ctgttcattt tgctagtccg
     7741 ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc taacagccaa actagcacct
     7801 tatgtgggaa cgatccattt tatcgaagtg ccatttaccg agattcaaga agaaatcaaa
     7861 aagtcagtgc cacaaggata ttggatgaca attactcgtc gaatgatgtt gcgtttgacc
     7921 gatgaaattc gccgtattcg tcatggttta gtcattttga atggtgaatc attaggtcaa
     7981 gtggcttcac aaactttgca aagtatggtg gctatcaatg aagtaacgaa tacgccaatc
     8041 attcggcctg tagcgacaat ggataaactt gaaatcattg aagtagcaga acagatcgat
     8101 acatttaatc tttctatcca gccttttgaa gattgctgta cgatttttgc tcctccacag
     8161 ccaaaaacac gccctcaact ggaaaaggtc catcaatatg aagagcgttt agcaatagat
     8221 gaaatgatcc aacgagcact agctggcttg aagatggaag agatacaagc agaatcgtct
     8281 aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg ttatgaaaat agtgttttga
     8341 ccagagtatt agagaaacaa aacgaaaaaa tagctccatg tattttaccg tttttgaact
     8401 aatgtcgaag attagctgat tgaataccat ttatcaagaa gttatggaaa agccggttct
     8461 tttttactaa ctgaaaaata aaattttatt tagagatcaa gctacagttg ataaatgata
     8521 taaaaaagag aatttcccat gaaatagcga ggttgtgaca gaagtgggca gcttcaagaa
     8581 ataagaagga atttacgaaa attgcttttc aaatttttgt gaaatttcgg cttatttttc
     8641 gaagaagtta cttctggaac accgtgtatc agaggttgcg aaaaagctgc cctgcatcaa
     8701 gtaataagaa cagccaaaca aaaatagttc ctcatatttt acgtttggtt gagcttatta
     8761 ccgcagatgc aagcttttga acaccgtttt atttgtctca cacccttttc cttcctactt
     8821 gtaaccttgc tcccgtgtta gaaacatgtt acactaaata tgtgaaatta ttaacaagga
     8881 gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc aactgcgaaa agattacctt
     8941 tatatttacg ctacttgaag atgctggatg actcaggtat aagtagaatc aaatcaaatg
     9001 agttcagcga aatcacccaa attccttctg caacgatccg cagagacttc tcgcaattag
     9061 gcgagctagg cagaagcgga tatggctatg atgtcccttt tcttatcgat gtattcaata
     9121 atatccttaa tactaaagaa gaaaaacgaa tcgcattagt aggatatgga aatcttggca
     9181 aagcattgaa acacaacaat tttagaagaa atgaaaatct aaatatcgtg tgcgtctttg
     9241 ataatgatcc agcactgatc aatcgagtaa ttgatggaga aatgatctat ccgattgatc
     9301 gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc aatctctacg gtaccaagta
     9361 aatattcaca atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt
     9421 ttgcgcctga cagagtaact gtcccagctt atgtcaatgt tcaatacatt gatttgacaa
     9481 ccgagttgca gacgcttatt tactttgatg agaattactc agaagttttt tcttaggaaa
     9541 atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag
     9601 tactaaatga agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga
     9661 atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta
     9721 tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat
     9781 ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta
     9841 ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac
     9901 gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata
     9961 taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa
    10021 accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac
    10081 tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca
    10141 aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc
    10201 gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg
    10261 tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg
    10321 cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga
    10381 tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat
    10441 ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat
    10501 cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga
    10561 tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc
    10621 gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct
    10681 acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg
    10741 gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg
    10801 tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg
    10861 aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga
    10921 aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac
    10981 aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc
    11041 gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt
    11101 caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt
    11161 atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg
    11221 gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg
    11281 gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga
    11341 ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg
    11401 ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt
    11461 cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa
    11521 tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt
    11581 atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc
    11641 ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg
    11701 aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct
    11761 tactcgtttg aatgaaacga tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg
    11821 tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga
    11881 aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat
    11941 cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac
    12001 agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc
    12061 tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga
    12121 attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat
    12181 tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag
    12241 ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc
    12301 agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt
    12361 gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga
    12421 agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga
    12481 agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa
    12541 agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca
    12601 gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca
    12661 ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc
    12721 tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag
    12781 ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat
    12841 gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc
    12901 aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga
    12961 agctttatta
//
LOCUS       DACEZN010000071        12937 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEZN010000071 DACEZN010000000
VERSION     DACEZN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.071"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            196..1257
                     /locus_tag="IXH60_001589"
     CDS             196..1257
                     /locus_tag="IXH60_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1990512.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1260..2156
                     /gene="murQ"
                     /locus_tag="IXH60_001590"
     CDS             1260..2156
                     /gene="murQ"
                     /locus_tag="IXH60_001590"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1990513.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2170..4152
                     /locus_tag="IXH60_001591"
     CDS             2170..4152
                     /locus_tag="IXH60_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1990514.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4217..4663)
                     /gene="nrdI"
                     /locus_tag="IXH60_001592"
     CDS             complement(4217..4663)
                     /gene="nrdI"
                     /locus_tag="IXH60_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1990515.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4874..6298
                     /locus_tag="IXH60_001593"
     CDS             4874..6298
                     /locus_tag="IXH60_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1990516.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6479..7252)
                     /locus_tag="IXH60_001594"
     CDS             complement(6479..7252)
                     /locus_tag="IXH60_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1990517.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7249..8154)
                     /locus_tag="IXH60_001595"
     CDS             complement(7249..8154)
                     /locus_tag="IXH60_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1990518.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8308..8628)
                     /locus_tag="IXH60_001596"
     CDS             complement(8308..8628)
                     /locus_tag="IXH60_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1990519.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            9102..9788
                     /locus_tag="IXH60_001597"
     CDS             9102..9788
                     /locus_tag="IXH60_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1990520.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            9790..10734
                     /locus_tag="IXH60_001598"
     CDS             9790..10734
                     /locus_tag="IXH60_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1990521.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            10933..11577
                     /gene="pcp"
                     /locus_tag="IXH60_001599"
     CDS             10933..11577
                     /gene="pcp"
                     /locus_tag="IXH60_001599"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1990522.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            11656..12933
                     /locus_tag="IXH60_001600"
     CDS             11656..12933
                     /locus_tag="IXH60_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990523.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         4105 a         2281 c         2763 g         3788 t
ORIGIN      
        1 atttaaataa aatatttcaa ggcactgctt ttgcttagca gtgccttttt taggttatga
       61 caattctttt gataatataa actcaacctt tagaaatgaa atttaattat tttaataaaa
      121 gtattgaaat tttgtttctt tagatatata ctctagtcat agaaatgaag acggatacag
      181 aaaaagaggg ataatatgtt tggcttttca atatttatga acgattcttt gtcagaagag
      241 aagaaacagt atatccgcca aatggcatcc aatggctttg tcggtatttt cacttccatg
      301 catataccag aagatgatgt ttcggcatat caaaagcgat tgatcgaact aggaagcgaa
      361 gcgaaaaaga ataaactaga attgatggtc gatatttcag gagaggcatt ggaacgagcg
      421 ggtttttcat tagaagatct aacgcctttg aaagcaattg gtgtaacagg tttaagaatg
      481 gattatcata tttccaatca gcagattgcc gactggtcac atcaattgaa aatcagtttg
      541 aatgctagca cgatcacgcc taaagatatc gatgaattga aagaagcaga agctgatttc
      601 tcacagattg aagcttggca taattattat ccacgtcccg aaacagggct ggacaaagaa
      661 tggtatcaga aaaaaaatca atggctcaaa attcaaggtc tccttgtcca aggttttgta
      721 ccaggagata cagagttgag aggcccacta tatcaaggat tgccaacttt ggaagaacat
      781 cgagggatcc atcctcttgc aggcgctcta gatctattag caagtaacac ggatattgtt
      841 tacatcggag atgcaggtct gtcagaaaac gttcaggagc aatttgcttc tttccaaaaa
      901 gaacagacag tcttgctcca tacggaacct gtagatgaag aattttacga atatatatta
      961 gggaagcata cgaatcggca agacgatgca agagatgtca tacgaagcgt agatgcacgt
     1021 tttcgagaga ttccgcctat cccagcaaga aatacagcta cgcgtatgaa agggtctatt
     1081 actttagata acgaaaagta tttgcgctat atgggtgaga tccaattaac caaatatgat
     1141 ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga gctaccattg
     1201 atcaaccaaa tcaaagctgg tatgaactat caatttataa gaaaagaagg aagataaaaa
     1261 tgagcaaaat caatttagag aacctaacaa cggaacgcag aaaccaagaa acttttggat
     1321 tagatgagat gagtatttct caagccgttc aactaatgaa caaagaagat cagaaagtgg
     1381 ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac acgattgagt
     1441 cctttaaaaa aggtggacgt ctgatttata tgggagccgg aacaagtgga agactaggtg
     1501 tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga accggaaatg gtcataggtc
     1561 taattgctgg tggagaagaa gcaatgactg tagctgtcga aggggccgaa gattcttatg
     1621 agctagggca agaagatcta atcaagctga atttgacaaa aaatgatata gttattggta
     1681 tcgcagctag cggtcgtaca ccatatgtga tcggtggatt agattacgca aaatcgatcg
     1741 gtgctgtcac aggaacgatt tcttgcaata aacaagccga aatcagtaaa cacgcggatt
     1801 tcccaatcga agtcgatgca ggtccagaat ttttgacagg ctctactcga ttgaagtcag
     1861 gaacagcaca aaaacttatc ttgaatatga tttctactat cagtatgatt ggtataggta
     1921 aagtattcaa taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta atcgaacgct
     1981 caaaacgaat catcatgcaa gcgacagaag ctgattacga aacagctgaa aagtactttg
     2041 aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat agtgataaag
     2101 aggaagctca agcaaaatta gctgaagcaa acggatttgt aaaagggacc ttatgagggg
     2161 gaaaagacca tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca aatttataaa
     2221 ggggcaggcg gcatgggcaa tgtagaaagc attgtccact gcatgactcg tgtgcgtatg
     2281 aagatcatca atgatgatct tgtaaatgtc tctgaattaa aagcgattcc aggagtgctt
     2341 ggtgtagtag aagatgaaca gcttcaagtc atcatcggac ctgggaaagt gaacaaagta
     2401 gcacagcaaa tggttgattt ggcaggcgtg aaattaggag aaaaactttc ttctgtaagt
     2461 acagctacag caaattcatc agcatctgga aaagaccgtg taaacgaacg ggcacaagaa
     2521 atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt attaaaagac
     2581 atttctaata tctttgtgcc aatgattcct gcatttgtcg gtactgggat cgtggcaggg
     2641 attgccgcag ttttagcaaa cttagtcaca gccggaacac ttgatgcagc aacatggcaa
     2701 caatatatcg atgtgatgaa catcttgaaa aacgggatgt ttagctacct agttatctat
     2761 acaggtatca atgctgcaca agtttttggt gcaacaccaa ctcttggtgg agttatcggg
     2821 gccgttgtga tgctgacagg gatgaatccg gaagcaccac tgaaaaactt atttactggt
     2881 gaagctcttg cagctggaca aggtgggatc ctaggtgtca tcttcgctgt ttggctattg
     2941 tcaatcgttg agaaaaaact gcataaagta gtacctgatg cggtagatat catcgttaca
     3001 ccaactcttt cattgattgc catcggtctg attgaaattt tcttcatcat gcctttagca
     3061 ggtttcattt ctgatggcat ggtcggtggg atcaactggg tacttggcgt tggtggagca
     3121 tttgccggct tcgttctagg cacattattc ttgccgatgg ttatgttcgg actgcatcaa
     3181 attttgactc caatccatgt gcaaatgatt gatgaaactg gtcgtacgct attgcttcca
     3241 attcttgcga tggctggtgc aggacaagtg ggtgcagcat tggcattatg gattcgctgc
     3301 aaaaaagata aagaattaac agaaatgatc aaaggagctt taccagtcgg gatcctagga
     3361 atcggcgaac cattgattta cggagtaaca ttacctctag gacgcccatt tattactgct
     3421 tgtatcggtg gaggtatcgg cggggcagtg atcggaatga tcggaaatgt cggggctatc
     3481 gctattggac cttcaggagc cgctttgatc ccattgatct ctgacggtaa atggttaggc
     3541 tacatccttg gattattggc agcttatgct ggtggcttcg ttgctacatt cttcttcggt
     3601 attcctaaag accgtttaga aaaagaagaa ttagcgactg aaacagccac agtaaatgaa
     3661 gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg tgactatcct ctcatcagtt
     3721 gctgacggaa cagttgtttc tttagataaa gcaagtgatc cagtttttgc acaaaagatg
     3781 atgggtgaag gctactttgt agaaccagaa aacggacata tctattctcc agttactggg
     3841 gtagtcagct ctgtcttccc aacgaaacac gctattggga tcacaacgcc aaatggttta
     3901 gaaatcttgc ttcatatggg aatcaacaca gttgacttag gcggtacgcc atttgatgtg
     3961 aaagtaacag aagggcagca agtgacacct gatacgcttg tagcagaagc tgacttagaa
     4021 gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa tatggatcgt
     4081 gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc tgtgatggat
     4141 gtcaaagctt aatatgatag aaacaataga aacgaaagaa gaaactgaaa tctatttttc
     4201 agcttcttct tttttattat ttattgtttt ctgctaaaat ctgataaatt cgtgtcacat
     4261 cagatggtgt tccccgtaat tcataatctg ccaagaaagg aaagccaaat gtttgtgcat
     4321 attgtttggc tgttagacaa tactgattat tgaagttttt atttccgcta ccgactacac
     4381 caagacaaag tttatgattg tcttcatgtt ccagatattc acgcatcgtt tcggtcaaga
     4441 tttctgtatc accgttatct aaaccatttc cgccgtctaa ataagtagga acgaaagtaa
     4501 aaaatggttc tgtttctttc gtaaaatcac tattttcatg gatttctttt aaagtaactt
     4561 caggattgac aggatcttct gcatgcattt tttcagcata ttctgctaga tgtttggcaa
     4621 aggcacgcgt atttcctgaa atcgatatat ataaaatgtt catggatcat cctccaattc
     4681 tttcctttta gtatagcatc ttttttaagt tttttggtgg tgtaatttgg aaagggacta
     4741 ggcgctttgc ttgtataatg agaaaaaagc cggtagaatt atatttctat taaagttttt
     4801 attaattaag taaaaataaa aaaggtttgt tatacttttt gataaaagcg gttttatttt
     4861 ggaggatgct tacatggata cagagcaaca gaaaaaagga attggtttaa ttccacttgc
     4921 tgccttagta attggttcag cgattggagg tggcgtattt ggtattatga cggacatctc
     4981 cggatcggct ggcggcccag ctattttcag ttgggtactc gtaggaacag caatgttgat
     5041 gctctccttg tcgatcaata atatcaataa aaaacgtcca gatttaaaag gcgggatctt
     5101 tagttacgca gaagaaggtt ttggccgttt cgctggtttt atcagcggtt ggggttactg
     5161 gctcacatgc tggttaggca acgtagcttt tgcaacattg ttgatgagcg cattaggata
     5221 tttcttccca gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga gtacagtctt
     5281 tttatgggga tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt tcgtcaatgc
     5341 tatcgtcaca ttctgtaaat tagtgccatt atttttattt attgtagtag ctatcagcac
     5401 gttttcgatc caacaattca ttgataactt ttctagcgac attatattga atgcgaatgg
     5461 acagccgatc ccgttttcca gtcaagtatt gagttgcatc atggtgatgc tttgggtatt
     5521 tgttggtatt gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat cagatgttgg
     5581 gaaagcaacg attctaggga tattcggctt gattattatt tacgtcttgg tttccatgct
     5641 tccatatggg acaatgacga tgaatcagct gcaaacgatc aaggcgcagc ctgctatggg
     5701 ctatgtgttt gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg gcggtttgat
     5761 catttcttta gttggtgttt ggctctcatg gacgatctta ccgattgaaa caatgggaaa
     5821 catggctgaa gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa atggggcacc
     5881 aacttatgct atcgtattga cgacgttatg tacgaatgtc tttttacttt cattattatt
     5941 cactgacagt gcttataact ttgcttacac acttggtaca gcagctattt tcttcacgtg
     6001 gcttttctta ggattgtacc aaatgaaact ttcttatcag cggaaagaat gggtccaatt
     6061 tttagtaggg cttttagcaa gtgcctttca agtatgggct atgctagtcg tagcctttaa
     6121 tgaagtaatg cttgttcttg ttcttttctt gccaggcatt tatttttacc tcaaagcacg
     6181 taaggagcaa aacgagcctg tacaacatat ccacttagat aagattttac ttataggtgt
     6241 gacgatggtc ggagtgattg caatggtttt atttgccact gggatgcttc acgtatagaa
     6301 gtataaaaga agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa catactagga
     6361 aaggagctgt gacacaaatg ttgtcacagc tcctttttca gaataaacgg tgtttaagag
     6421 acagccaatc ggcaataagg ctgaacgtct ctttcacagc ctcttttttg atgagtcatc
     6481 atacattttt tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg atgtaagcta
     6541 aattaccgaa tagcatttga ttagtagaaa gatgagtcaa ttgcttcatg atctcttcgt
     6601 ttccaatttg gttttgcaga ttcaacatac tgatcggatt ccatttgatc cactcccatt
     6661 tttggatgag tgcaataagg ataccagata tgattgacga agcaaaataa aagacgattc
     6721 ctgctgagat ggatgcgccg gaactattga taaagcaagc aatcatcaac actaaactta
     6781 aaatcagcca taaaccgata taactaccca aagtataagt gaacaagctt tggatcagtg
     6841 tttgaccagt agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc agtttcatca
     6901 acacagtgac aataatagct aaaaggtata aataaacaga ataaataact aatgtgatcc
     6961 atttgcttac taatatttct cctcgagaat actttcgata aaggagattt ttcaatgtac
     7021 catattgtgc ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata aagacaatcc
     7081 aagatgtggc actaaaaagc tgagcagtca tttcaactgg atcaatgatc gtcgtgtatt
     7141 ttttggctag aagtgctgtt cctatgagca aaacaactaa tagaacagat aaaacagcag
     7201 ttgacttttt cttgatcaat ttaaataatt cttgttttac aagcaatgtc atttttcttc
     7261 ttcccccttt actgaatcca gaatttccaa tagagaagat tccaaatcat tcgtcagatg
     7321 ctggacgtct gtcacatgga tattttgtgc agacaatagc tggatcacgg ttcctaataa
     7381 ttcttcgttt tcttttaaaa gcttgatttt atcaggatct tccaaagtat atccagcttc
     7441 tgtcaagatc gttcgagcag ctagatcatt tgttgtagag ataacgatga aatcttgcgc
     7501 tgtttggttc aattcagcca ttgtacattg tttgatgatc tttccttgat tgatcaggat
     7561 cagctcatcg accatttttt caagctcgct gagtaagtga ctggagatca agaaagaggt
     7621 gccctgagta gccaagtcgg taatgatttt tcgcacatct cttgttgctt gtggatccaa
     7681 accgttcatc ggctcatcta aaataacgat agacggatgg ttcagtaagg caagagcaat
     7741 tcccaatttt tgtttcattc ccaacgagta ggatttcgct tttttgtgaa tgtacttttc
     7801 catcttgagc tgattcacga tagtgtcgat agcagataca tcgttggttt cagagaataa
     7861 cttcagatga tcatagcctg ttaagaatgg atagataccc gggtattcga tcaatgctcc
     7921 tacttgttcc aaccctttat gactagaggg agaaatctca ttttcaccaa tacagattgt
     7981 tccatctgag taatggatca gtcctaaaat agctttcatg atcgttgttt ttccagcgcc
     8041 attaggacct actaatccaa cgattttgcc aggggataag ctaaaactga catcgctgag
     8101 ggcttgtttg tgaccaaagt tcttggataa ttcttggacc gaaagacttt tcaaaaatat
     8161 tcactccttt tctatagata gtacaagcat acacctgatg agtggagaag tcgctaggtc
     8221 attggactta tttcttattt tttcataaaa aaattgaata ttaaataaaa aggcttattt
     8281 tttctgatca gtacttattc caagtgtcta gtggccgcta gtaatcttga tgccaatgat
     8341 actgatgagt agcatcccca caaagaacca agtggaaaga ttcagcttat catgaaacaa
     8401 gaccacacca attatgaccg aaccaactgc gccaatccct gtccagatag gataagagag
     8461 actcatcggc aaacttttga ttgcctttga caaaaagtaa aaactagcaa tcatgccaat
     8521 cactgtgtaa aaagaataat tgattttaga aaaaccatca ctccatttca tcatcgttga
     8581 ccagaatact tccaaaattc ctgctatgat taattccatc catgccatac ttacattcct
     8641 ccttaactat aaaaaaatac acaaaaagag gaaaatcatc ccttcaaaga cctaatggaa
     8701 tgatcatcct ctttatccta cccggtggca gattgtatat ctttttctgt agtatagcac
     8761 acctatccag atatcggcaa tctcatactt tttagtaaag tgtaccaagc actgacggat
     8821 atatcgttag cggaaaagaa gatgaatgct ttgcaatttc atagaaagaa gttgttattt
     8881 attaatcatt taaaaaaaac agtaactaga ccttatttct atatattatc gggcaataaa
     8941 ttaaaaaaag ctttacctta caataagtaa gataattttt gtgataaaaa aataacgaaa
     9001 aatatttaaa aatagtgtct atttcgtgag aaaacaacta tattttttat gtgttttgct
     9061 ttataattaa aaaaatattg tcattttatg gaggaaaata tatggaaaat tatcttagcc
     9121 ttttaggcgt cttgattgtt attctcgggt ttgcatttaa attagactcg atattgatcg
     9181 taatggttgc tttagttgtt acagctttga ctggcggtct tggcattcaa ggaatgttag
     9241 aacttttagg tacaagtttt gtaaacaatc gcgggatggc gatttttatc atcattatgt
     9301 tggcaacagg aacgcttgaa agaaatggtc tgaaagaatc agcggcttcc ttgatcaagc
     9361 gattcaagaa agtatctgct ggtatgattg tagatattta tgggatcttt cgaatgatct
     9421 ttgcggcttt taacgtgagt tttggtggag ttgctggatt tgttcgtcca atcattttac
     9481 cgatggcatt ggggaccatc gaatcaaaag ggttagaagt ccatcctaag catgaagaag
     9541 aactaaaagg tatggcttca gctatggaaa atgtctgctg gttctttggc caagtactat
     9601 tcatcggagg agcaggggcg ttgctagtcc aatcaacact gaaagatcta ggatatgacg
     9661 taacattggg acagcttgct ttagtggaag ttccagtggc tatcacggct ttagttgtgg
     9721 caagtgtcta cttctatctt aaagaaaaga aattagcgaa gaaatattat ccaggaaagg
     9781 ggcaataaga tggacttttt tacaaatcct gatattttat taggcgataa attactggag
     9841 atcctttata tcgtgatggg actcgtattg atttacactg gagtaagaaa tttaacagat
     9901 aaaaccaatc ctcatcgtta tgggtcagcc tttttctgga cggtcttagg ggtagttatt
     9961 gcaggaggaa gatggcttcc ggcaatcgtg aacggtggct tgatctttgc gatgaccata
    10021 ccagcaattg ctaagaaagt cagcaaagga gaaagccggc ttccatcaaa agaatatatg
    10081 gaaaaaatgt cagacaaact aggaatgaaa atttttattc cagctttgag tatcggtgtc
    10141 tttgcgatct tgtttgctct atttaccaat ttaggtgctt tagtcggcgt gggcgtcggt
    10201 gtttttgcag ccatgctaat cttgatgttc ttttctcgag ataatcgacc aagtacattt
    10261 ttagatgatg cagcagatat gctgggcaca gtagggccgt taagcatgtt gccaatgctt
    10321 ttagcttctc taggtgcggt atttacaagc gctggagttg ggacagtgat ttccaatgca
    10381 gtaggtacga tcgttccaga aggaaatgtg aacatcggca tcatcattta cgctcttggc
    10441 atggtgatat ttacggtaat catggggaat gcatttgcgg ccatcaccgt gatgacggtt
    10501 gggatcggtg caccattcgt atttagtcat ggagcgaatc cagcgttaat aggaatggtt
    10561 gcattgactt gcggtttttg tgggaccctg ctgacaccga tggctgctaa cttcaatatc
    10621 gtgcctgtag cgatgctgga gatgaaagac aaatacggag tgatcaaaaa ccagttgttt
    10681 attgcgttat ttatgctagt tttccaaatt gcttatatga ttctatttaa ataatagaag
    10741 agaggggttg tgacagaata attgtcgcaa cctcttctaa cattgtagtg gaacactatt
    10801 tttttataag aatatattaa gtttgatctg ttatgttatt taaactattg tttcctttat
    10861 ttacgctgtt caaggaaaat ggtatgataa aagataacca tatgttaaat caatcggacg
    10921 gaggaacaac agatgaaagt actagtaaca ggttttgatc catttggtgg ggacaaagtg
    10981 aatccagcct atgaagcagt aaaaaaaatg ccagatgaaa tcagcggtgc tgaaatcatc
    11041 aaagtggaag taccgaccgt attcgaaaaa agcagtcaag tagtcaaaga agccatccaa
    11101 cagcaccaac cagatgtcgt gatctgtgtc ggacaagcag gtgggcggtc agcagtctcg
    11161 ttcgaacgtg tcgcaatcaa tctagcagaa gcaagaatcc ctgataatga aggaaatcag
    11221 ccattcgata cggctttaga agaaaacggt ccagccgctt actttacctc attgccaatc
    11281 aaagcaatga caaaaaatgt acaagatcat ggtctaccag cgtacatctc ttatacagca
    11341 ggaacatttg tctgcaatga tatcatgtat cgtttgcttc atttgatcga aacacaattc
    11401 ccggcaattc gaggtgggtt catccacgtg ccattctcac ccgatcaagt gatcgatcgt
    11461 ccagtgggta cgccttctat gtctttagaa gacattgctt cttctttgac ttatgcagtt
    11521 gaagcagcag tagaaaataa agaagatatt tccggaaatg ccggaacaac tcactaattt
    11581 agcaagaaac actggctggt tgctaacgtt atgttagtag ccagcttttt gtaattagaa
    11641 aggaaagaaa cagccgtgga agaatggcaa tccgtctttg aagaatggtt tccaaaagaa
    11701 atcagcaaaa gctatcccat caaaatttct aaacaataca caagcagtca acgttgggag
    11761 atctatgcaa aattaacgaa aaaacagcgg gaactagtcg ataagcaccg ccgatatcta
    11821 atcagctctc gttttatgga agaacattat ttagctgcaa cagattgggt gttttctgac
    11881 ttcaagatca atccgttttt tcgaacaaaa cgaagccagc aaaaattgta ttgtgagtgt
    11941 ggtagagaat taaaagtgca atacatcgtt aaatcgccaa aaaccggaaa gatcctaaaa
    12001 ctaggcatca accactttgc cgatcacctg catgtctctc cgacagtggc agcttcgatc
    12061 catcagggaa tgacaaaagt tgatctggcc ttagacgaat tattatggct taagcagaag
    12121 aatatcgatt ttcctgaagg actatggcaa aaatactgtt ttgtcttgta ccaaaatcgt
    12181 cggatgaaac agccatattt accggatata aaattagctc agcggctcgc agaatttcgc
    12241 caagtagaaa tgccgattta catagcggat tatcaagcat tggaaaatga aatcaagaaa
    12301 ataagtgaac acatcaatgg gcagtcaaaa aagcgacaga taaaaaaaga actatttgac
    12361 gactttgcag aagaattagt aaaggatgta gaagaattct tgatcaacta tcgcgcattt
    12421 ttacgtaaag attggcaatc catcgtatat gaagaagtgc cagtacatcc taatgcttat
    12481 tttgaaacat ttatttccgt gttgagaaag acaaaacgtc aacgaacacc agaagtcact
    12541 gctcagatgg aatattttgc gaaaaaccaa cgattcatcc aaccaaaaat ctatctattt
    12601 atttggaagc aatattgtcg ctatggtttt acagaagggt tcttcgacag cattcctcgg
    12661 atagtcagaa atggtttttt aaaagtattg cgtaaagaac gagaagctat acaatctgct
    12721 gataagaaag accgtacagt ctcaaaagaa aaatggcaac tagtagtgaa agatatccaa
    12781 tctggaaatg tgcaagagac aatcgacaaa tggaaaggaa aacattatcg ttttactgaa
    12841 gctcagaaac aagcgttgga atattatcag aagttagaag aatcactgcg tttcaatgat
    12901 gaggcaagaa agtatctgaa ggaattacta taaaaaa
//
LOCUS       DACEZN010000072        12855 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEZN010000072 DACEZN010000000
VERSION     DACEZN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.072"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..486)
                     /locus_tag="IXH60_001601"
     CDS             complement(<1..486)
                     /locus_tag="IXH60_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1990524.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EP"
     gene            621..1010
                     /locus_tag="IXH60_001602"
     CDS             621..1010
                     /locus_tag="IXH60_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990525.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1014..1778
                     /locus_tag="IXH60_001603"
     CDS             1014..1778
                     /locus_tag="IXH60_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1990526.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1907..2854
                     /locus_tag="IXH60_001604"
     CDS             1907..2854
                     /locus_tag="IXH60_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1990527.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            2984..3724
                     /locus_tag="IXH60_001605"
     CDS             2984..3724
                     /locus_tag="IXH60_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990528.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4015..4683)
                     /locus_tag="IXH60_001606"
     CDS             complement(4015..4683)
                     /locus_tag="IXH60_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1990529.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4817..5104
                     /locus_tag="IXH60_001607"
     CDS             4817..5104
                     /locus_tag="IXH60_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990530.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5520..7058
                     /locus_tag="IXH60_001608"
     CDS             5520..7058
                     /locus_tag="IXH60_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1990531.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7055..8020
                     /locus_tag="IXH60_001609"
     CDS             7055..8020
                     /locus_tag="IXH60_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990532.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8007..9329
                     /locus_tag="IXH60_001610"
     CDS             8007..9329
                     /locus_tag="IXH60_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1990533.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9392..9496
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9570..10313
                     /locus_tag="IXH60_001611"
     CDS             9570..10313
                     /locus_tag="IXH60_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990534.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10328..11320
                     /locus_tag="IXH60_001612"
     CDS             10328..11320
                     /locus_tag="IXH60_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1990535.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11332..12069
                     /locus_tag="IXH60_001613"
     CDS             11332..12069
                     /locus_tag="IXH60_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990536.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12059..12781
                     /locus_tag="IXH60_001614"
     CDS             12059..12781
                     /locus_tag="IXH60_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1990537.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         4288 a         2189 c         2669 g         3709 t
ORIGIN      
        1 tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc
       61 acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat
      121 tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc
      181 ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc
      241 ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt
      301 cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc
      361 gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa
      421 cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc
      481 aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga
      541 agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat agaattataa
      601 agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc
      661 taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag
      721 agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt
      781 tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt
      841 atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg
      901 agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg
      961 gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc
     1021 caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat
     1081 taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt
     1141 tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg
     1201 cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg
     1261 atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag
     1321 gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg
     1381 ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac
     1441 ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa
     1501 aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa
     1561 agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata
     1621 tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt
     1681 ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa gaagcttcca
     1741 cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg
     1801 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
     1861 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
     1921 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
     1981 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
     2041 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
     2101 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
     2161 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
     2221 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
     2281 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
     2341 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
     2401 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
     2461 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
     2521 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
     2581 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
     2641 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
     2701 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
     2761 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     2821 tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg
     2881 aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt
     2941 gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg
     3001 aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa
     3061 gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt
     3121 tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg
     3181 tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg
     3241 aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa
     3301 actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca
     3361 tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa
     3421 atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt
     3481 aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc
     3541 gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt
     3601 agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg
     3661 ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa
     3721 ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttcc
     3781 ttattttata ctctatccga aagtttatat cgtatcctca aatttttttg agtttaagcg
     3841 aattttttct cccaataagt aagaaaataa tggcaaaata aaaaaacaaa ctattttcgc
     3901 agatcgtttg caaaaatagt ttattttttt ataaagaaga ttttactgaa taatatcatt
     3961 ttagtttaca taatgatatt tctgtaaatt aaataaagta gtgaaaaata agaatcagtg
     4021 ctctaactgt tcaagaggat caagtggttc aaatgtttct gtagtctgtt ttgcttccaa
     4081 ttggggcaaa acagatccaa tttccagata cttcacttta ttcaatgtat aagatagaat
     4141 agaacagcta agtaatgtat acagaaaatg tgtgagatcc aaataaagca aggagacaac
     4201 taaaatacca aaagtaatca agaaaagact tttgccaatc ttaatatgaa aatatttttc
     4261 aatggcttga taaatgatat ctgttccgcc tgtgcttccg ccgaatcgaa agacgagacc
     4321 gcaaccaatg ccagaaagaa tacctgtcat caaactattg atcagtagac tatctacagc
     4381 aaaatgtata tgaaggctct cccataaatg taaaaagaaa actagcgcaa atgttccgac
     4441 gatcgtccgg agaaaagcgt gttttccaaa aagaaacaat gagataagca ataaaggtaa
     4501 attaaaaagc agtgaagtga cagaaggagc gaatccaaaa agattatgca aaagcaaagc
     4561 aataccagta accccaccat ctgccagctt agatggaata ttgattcctg tcacagctaa
     4621 tccgtagatt cctgcgccta aaatgattcc gcacaattct gtaaataaat ttaatttttt
     4681 catttctagc ctcttttctt ttgaaaattt tcagcaactt ctttattata ctgacttata
     4741 ctcttacttc aagaggaaaa tgaaaaaaaa ttatttttgg ctgtttcttt ttatttatca
     4801 aataaggagt ggaaaaatgc gagcagcaga cttattgaca attcttacaa aatcacccaa
     4861 aaaaacagag tatcaaggag tttttttggc tcaagaacaa aaactggtgc ctgtgacaat
     4921 ggtccagata aataaagcaa atcaatttat ctttttattt gaacacggca aagcaccact
     4981 tgctatgaaa gacatctttc tttttttgat gaagaaccgt gagaaagctg tttattatca
     5041 gtcaaaagaa gaactcattc ctctttatgg agtgaaagag atagaaaata aagtggtgat
     5101 ataagtaaaa ataagaaaac aagacgttta tgatgtcaaa acaaccattt tttacatttt
     5161 tggcaaaaaa gtgtcatgat acagtataat attcgttgaa caggccaata aaggtgttct
     5221 cccccacgag atttctactt tattggctgt ttctttttat taaaataaaa gttgtgtgtg
     5281 taaaaaagat atagctgatt ccaagtgaca atttcgtata gatttttatg gtattcgtag
     5341 tgaaaaaacg agaaccaaaa agttgatccg actttgttgc ttggattctt tttttgtcag
     5401 catatttaga ggtatttttt tctttttttt gttcgtgctg taaggttaag gcaagacaag
     5461 ttagttggga aaaaggaaag aaatgtttat acttaaagaa taacagaaag gaaggtcgca
     5521 tgaataattt tatacaaaca ttaagtgaaa gaaaaggcga gttgctggaa gctgcatttc
     5581 aacatctgtc catttctctc atttctttgc tgattgcagc actgattgct attcctttag
     5641 cgatttgggc agccaatcat aaaaaaatgg cagaagtctt attgcagatc acgggtgtgc
     5701 tccaaacgat tccttcactt gccttattag gtttacttat cccatttgta gggatcggaa
     5761 ctgttccagc attgattgca cttgttattt acgctttgct tccgattttc caaaacacgt
     5821 atattggtct agctgaaatt gacccgtcta ttgaagaagc agcggtagca tttggtatgt
     5881 ccagaatgag acgcttgctg aaagtagagc ttcccatcgc attacctgtc attatctcag
     5941 gtatccgaac tgccttagtt ttaatcatcg gtacggctac tttggcagca ttgatcggag
     6001 caggtggatt agggacattt attttgttag gtattgaccg aaatacacct gttttgacat
     6061 tgattggtgc gatcagttct gccttgcttg caattatatt cagcggactg attcgctggt
     6121 tgcagcataa gaaacctcga tattccatca ttactttagc agcagtggtg tgtttatttg
     6181 gcggcgtttc tttatatcag tctgccttgt ttaaaaatga aaccatcacg attgctggaa
     6241 aattaggagc ggaacctgaa atcctgatcc aaatgtataa agaattgatt gaagacaaca
     6301 caaaaacaga agttgaatta aaaccgaatt ttggcaagac gagttttttg ttcaatgcac
     6361 tggatcaagg tcaaatcgat atctatcctg agttcacagg tactgtccta gaaagcttgg
     6421 tgaaggtacc tgaagcaatg aaaagtgctg atttgacaaa agaaaaaaca tatgacgaag
     6481 cagaagaatt attgaataag caattcgata tgacattatt aaaaccaatg gcttaccaaa
     6541 atacgtatgc cttggcagtc aagagatctt ttgcgaacga acatcaactt aaaaagattt
     6601 ccgatttggc agctatctct gatcagatca aagcgggatt cacactggaa tttattgatc
     6661 gttcagatgg atataaagga atccaagaag catatggctt gaatttttca agtgtacaaa
     6721 gcatggagcc aagtttgcgt taccaagcaa tcaataatgg agatgtcaat attgtggatg
     6781 cctattccac agatagccag ctcaaggagt acgatctagt gactctagaa gatgatcgag
     6841 ggttattccc agcttatcaa ggcgcaccgc tgatgaaaaa agattttgct aaagaacatc
     6901 ctgaaatcgt taaagcactg aataaattaa gcggaaaaat aacagaggat caaatgattg
     6961 atatgaatta ccaagtcaat gtcgaaaaga aaaagccgac agaagtagca cgaatcttcc
     7021 tgaaaaatga aggattagta aaggaggaag atcagtgact aaattaatcg agttcaaaca
     7081 tgtgcaaaaa aagtatgatg gaaagtatgt catcgatgac ctaaatctgg caatcaaaaa
     7141 aggagaaatc tttgttttgg ttggtccttc cggtagtgga aaaacaacta ctttgaaaat
     7201 gatcaatgga ttatctaaac catcagcagg agacatctac ttcaaaggaa aatcgttaaa
     7261 tgaatataat ctgcagaaaa tgcggtggaa tatgggatat gtgctgcagc aaatcgcttt
     7321 gttcccgacc atgacagtca agcaaaatat tgaagtcatc cctgaaatgt taggttggga
     7381 aaagcagaaa agagcagacc gtgtagatga acttcttcaa aaagttggct tatctcctga
     7441 tatttatcga gatcgtatgc ctagagagtt gtctggagga gaacagcaac ggatcgggat
     7501 catccgagct attgctgcaa gcccagatgt gattttgatg gacgagccat ttagcgcatt
     7561 agatccgatt tcaagaaact cattacaaga attggttttg tccttacacg aagaattggg
     7621 cacgaccatc gttttcgtta cgcacaatat ggaagaagca atcaagctag gtgatcgtat
     7681 tgcatttatg aaggatggag aaattatcca atgtgacaca ccagagcagt tattgatgaa
     7741 tcctaaaaac gactacgttc ggcatttctt tgatgaaccg aaacagacga aagagtggcg
     7801 agtagaagat ttagtcgtca atggatattt cttaaatgaa ataccagaaa atgctcgtca
     7861 acaagtttgc tttgatacgc ctatgaaaga agtatattcg cttctttcgg tgtatccaag
     7921 cattgtgata gtagaaaagc aaagaagtat tgggtcgctg acaagtaaag aaatctttgc
     7981 ttttttaagt cgagaggagg aaggaaatga aaacatatga tgcaatcatc atcggttcag
     8041 gtgtgagcgg cttaagtgct gcttatggat tgaaagaagc aggtaaaaca gtactagtgg
     8101 tcgaagaaga tctatggggc gggacttgtc ctaaccgtgg atgtgatcct aaaaaagtct
     8161 tgttaagtgc agtagaggca cgtaatcggg taaaacaatt gagtggaaaa ggttttaacg
     8221 agatcccaac tgccaactgg gaagaacttc aaaaattcaa acgaacattt acggatccag
     8281 tcccagaaag ccgaaaaaaa caattagcag aagctgaaat cgatcatctt tcaggaaccg
     8341 ctcgtttttt ggatgattca tctattgaag tcaacgaaga agtcttccat gcggattact
     8401 tagttttggc aaccgggcaa agacctacga tcttaccagt ggagggaaag gaatacttga
     8461 aaactagtgc agacttttta tcattgcctg tactgcctaa agaaatcatt tttatcggtg
     8521 gtggttatat tgcttttgag ctagctacga ttgccaatgc tgcagggagc aaagtaacca
     8581 ttgtccatca taatcaacgg ccattaaaag aatttgaggc gtctttggta gaggaagccg
     8641 tccatcaaat ggaagcatct ggcattcagt ttgcttttgg tgttgaaacc caaaagatca
     8701 tttcagaagg tactcgctat cgtttagtcg gaaaagagac ggaattagta gcagatatga
     8761 ttttttgtgc gactgggaga cagccgaata cagaatcttt agcacttgag caagcgaata
     8821 tcgtctttga taaacacgga attgcagtca atgattatct gcaaacgagc aatccaaaaa
     8881 tttttgcgtg tggagatatt gtttcaagga aaaccccaaa attgacacca gtcgccactt
     8941 ttgaaggaaa ttatgtagct aagcggataa cggatgcaac aagcgaacct atcaaatatc
     9001 cgattatacc tacgatcgtc tacgccagtc ctaaacttgc tgaagtagga gttacaaaaa
     9061 gtcacgcctc ttcgtcagat caagtggtag agatggatct taccagttgg ttcacctatc
     9121 atcgtgtcaa tgaaccagtt gctaaggctg aattgacctt tgaccagcaa aattatttga
     9181 ttggtgctgc agtgattagc gagcaagcag acgaattgat cgatgatttg acactcgtca
     9241 tcaatcaaaa attgactaaa aaagagttgg atagctatat catggggtat ccgacattgg
     9301 caagtgatct ctcctattta ttgaaataat cagcatgata actaaagcgc ttgttgcttt
     9361 ttccttcttg tgctacactt taaaaaatga atacctgttt ctggggatgt cgtagacgac
     9421 atgagagtaa gttgtgaact tagacccatt gaacctgtca gttaatactg gtgaagggag
     9481 aaacatagca gacgcttgaa gcgggttact atgtcgtgcc catttcaagc gttttttgtt
     9541 attcatatta gaagaaagga ggtaacaaaa tgaagaaaaa tctttatcca gcagtcagta
     9601 tggcattcat tttaattatt tggcagattg ttgtcatagt ttggaaaata cctgccttta
     9661 ttttacccgg acctgctgct gtattttctg cattagcggc agatgcacaa gtattacttt
     9721 cccattctgt cacgacatta aatgaagcag tgcttggttt ggtaatagct agcgttcttt
     9781 cttttattac tgccttggca atggattatt ggaagttttt agaaatgtct ttttatccgt
     9841 tactagtgat ttcacaaatg ttgccaatca tggtgttagg gccattactt accctatggt
     9901 ttggtttcgg tatgcttcca aaagtgatcc ttgttgtctt gataagctat tttccgatcg
     9961 tagtttcttt ttcggataca ttgaggaaag tatcaaaaga gcaaatcatc tttttgaaaa
    10021 cgatgggggc agatacaggg aagatttatc gaatatataa aataccagcg ggtttatccg
    10081 gcttcttttc cggtttaaaa gtggctgcta cttattgtgt cagcggagca gtcgttggag
    10141 aatggctaag cgctcaagca ggcttagggt attacatgat acgcgtaaaa aacagctacc
    10201 agatcgataa agtttttgct gcgatcattt gtgtcattct attgagttta ttgctgaatg
    10261 gcgcttgttc attattgaaa aaagggtatt atcgtatgct ttatattttt taagagaagg
    10321 ggatcaaatg aaaaaattat ggcttttatt accgctactg ttgcttttat ccgcttgtgg
    10381 cactgcaaaa gaaacaagta gcaaacaaga aataaaggat ttgaaaaaag tgacacttgt
    10441 tttggattat gtaccgaata ctaatcatac tggcatctac ctagcaaaag aaaaaggcta
    10501 ttataaagag gctggtttaa atgtccaaat catcgaacca ggagataatt cgacaagtat
    10561 tggtctagta ggtgcggaca aagcacaatt tggtgtaagt tatcaagaag atgtcactta
    10621 tgctcacgca gatggacaga atataccagt caaagcaatt gctacagtga tcaaacataa
    10681 tacttctgga ttcgccaccc tatcagacag taacatccat tcaccaaaag attttgaagg
    10741 aaaaacatat gcaggttggc aaagtccaag tgaagaagct gtcttaaaag ctgtgatgga
    10801 aaaagacggc ggggactttt caaaactgac aatggtagga agcaatggag aagggccaga
    10861 aagcttagga aaatcctcag atatccaatg gtactttgaa ggttgggata tgatcaaagc
    10921 aaaagaagcg ggtattgaac taaactatat cccattgaaa gaattagatg aacggttaga
    10981 ttactatact ccggtaatta ttacgaatga tcaactgatc aaaagtgatc cagaattggt
    11041 acagtctttt atggatgcca cgaaaaaagg ctatcaggaa gctattaagg atccaaatga
    11101 cagtgctaaa ctgttgcaaa aatatgcaaa agaaaatgat cggacgtttt tagaagaatc
    11161 acaagcattc ttgtcaaaaa attatacgga tgatcctgag aattggggat tgatggaaga
    11221 aaaagtttgg cgcaattata cagcttttat gcaggaaaac ggattgatca agcaagatgt
    11281 accttcgcaa gaactattca ctaatcagtt tataaaatag gagtgataga tatgtcaatc
    11341 caaatcagcc acttgaatgt agtcattgaa aacctgaaaa tattagaaga catccatttc
    11401 actatcccta aagatacctt tacatcgatt gtcgcgccaa gcggagccgg taagtcaaca
    11461 ttgctgaaaa ctttaacagg agtccagcct atgacttctg gaacgatcaa agtagacgat
    11521 caaaaagtaa ctggtctcca tacagcattt agttatatgc cgcaagaaga tatgttactt
    11581 ccttggctga gcgtctatca aaacgttacg ttatatcaaa aaatcaatca tctaacaatc
    11641 aatgaagaac aagtaaggga ttatctggat atctttgggt taaacggcta tgaacatttt
    11701 ttgccggaac aattatctgg cgggatgaag cagcgaacgg ccttgttacg aacaatcatg
    11761 aatccttctt cttacttgct tcttgatgaa ccatttggag cgttagatgc tatgaccaga
    11821 gggcagatgc aagactggat gttgaagctg cctaaaaaag caaagcggac tactttactt
    11881 gtgacgcatg atatcgaaga agcaatttat ttatcagaca ggattcttgt gctttccgcc
    11941 cgtccggcac atgtgattgc tgagattcaa gtgcctgaaa aacgacggtc acgtgaatgg
    12001 ctactgcagc aatcagagtt gaagcaaacg atttatcagt tattggcagg tgaattagat
    12061 gttaagtgat gctcattgtc atttagatgg gagtcagagt ttagcactcc ttcagcagga
    12121 acacacgatt ttgacgatca tcaattgtga ttcgccagaa gaatggaagg aaaacagaca
    12181 gcttgcagca agtaagactc aagccttaag ttacggtatc catccttgga aagcagatag
    12241 ctatacgttt gaacaagttg agcctttttt gaaaaaagca aggatcatcg gtgaaatagg
    12301 gttagataat atttggacga atgtaccgat gactacacaa aaaaaagtat ttgagcgaca
    12361 gcttgctttt gcagccataa atgagaaacc tgttgtactg cacacaaaag gatgcgaaaa
    12421 agagatctta aattatatcc aagcttaccc taatcactat ttgatccatt ggtattcttc
    12481 tttggactat caacaagact atattgattt aggctgttat ttcagcattg gtcttgattt
    12541 gaaaaagaat ccagcagtct ggcaactagc taaagcagtc ccaatcgacc acttacttat
    12601 tgaaacggat ggactacatg cagcagagtg ggctttaggg gggatttttt cagaaaaaga
    12661 ttatgtctca ctgcttgaag aacagttaaa gacaatcgct gagatcaaag agctatcagt
    12721 tgaagaagtt aaaaaaaata cttatcaaaa cttggaacgt ttcattagat taggtaaata
    12781 aaaaagagat gtgacacaac tagtgtcgca cctctttttg gctttctttg tataaaagta
    12841 aactgtttga ttaat
//
LOCUS       DACEZN010000073        12827 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEZN010000073 DACEZN010000000
VERSION     DACEZN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.073"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..243)
                     /locus_tag="IXH60_001615"
     CDS             complement(<1..243)
                     /locus_tag="IXH60_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990538.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(358..936)
                     /locus_tag="IXH60_001616"
     CDS             complement(358..936)
                     /locus_tag="IXH60_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1990539.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1013..1543)
                     /locus_tag="IXH60_001617"
     CDS             complement(1013..1543)
                     /locus_tag="IXH60_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1990540.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1547..2407)
                     /gene="rlmB"
                     /locus_tag="IXH60_001618"
     CDS             complement(1547..2407)
                     /gene="rlmB"
                     /locus_tag="IXH60_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1990541.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2400..2795)
                     /locus_tag="IXH60_001619"
     CDS             complement(2400..2795)
                     /locus_tag="IXH60_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1990542.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2792..4204)
                     /locus_tag="IXH60_001620"
     CDS             complement(2792..4204)
                     /locus_tag="IXH60_001620"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1990543.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4201..4737)
                     /gene="cysE"
                     /locus_tag="IXH60_001621"
     CDS             complement(4201..4737)
                     /gene="cysE"
                     /locus_tag="IXH60_001621"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1990544.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5059..6519)
                     /locus_tag="IXH60_001622"
     CDS             complement(5059..6519)
                     /locus_tag="IXH60_001622"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1990545.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6689..7846)
                     /locus_tag="IXH60_001623"
     CDS             complement(6689..7846)
                     /locus_tag="IXH60_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1990546.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7880..9250)
                     /gene="radA"
                     /locus_tag="IXH60_001624"
     CDS             complement(7880..9250)
                     /gene="radA"
                     /locus_tag="IXH60_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1990547.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9313..9840)
                     /locus_tag="IXH60_001625"
     CDS             complement(9313..9840)
                     /locus_tag="IXH60_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1990548.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10070..10876
                     /gene="proB"
                     /locus_tag="IXH60_001626"
     CDS             10070..10876
                     /gene="proB"
                     /locus_tag="IXH60_001626"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1990549.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10873..12120
                     /locus_tag="IXH60_001627"
     CDS             10873..12120
                     /locus_tag="IXH60_001627"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1990550.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            12196..12540
                     /locus_tag="IXH60_001628"
     CDS             12196..12540
                     /locus_tag="IXH60_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990551.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
BASE COUNT         3440 a         2794 c         2389 g         4204 t
ORIGIN      
        1 aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta
       61 agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata
      121 ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa
      181 catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg
      241 catacagttc acctctacta tcaactatac cacatatttt taaaattttc aagtttacca
      301 taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta
      361 ttcctcatta taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct
      421 cattacttgc ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg
      481 catataataa tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat
      541 tgcttctttt aataaaagat gctgaatcgc taaaggttct tgcaatgtga tctctgctaa
      601 aaagtctggt ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg
      661 acgcttcagc gcgttctgct tcctcaacag agaatagatt tgattgctta taatgctttt
      721 ataataactt ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa
      781 agcgatcctt gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt
      841 tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc
      901 accattacgt gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt
      961 ttatggagga atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat
     1021 tttttctcat taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg
     1081 gagaattacg tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt
     1141 ccgttttttt tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa
     1201 agatcatcca ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat
     1261 tcagctgacc agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa
     1321 caacatccaa ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga
     1381 agacaacgat gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag
     1441 cttctcttgc atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac
     1501 caatcatgtt gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac
     1561 gttgggaaaa agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga
     1621 cgtgcccggt catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca
     1681 ttcctttgcc ttcattacca atgactaaag caattgatcc tttggcattc catttttgga
     1741 agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca
     1801 cagcttgtac caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag
     1861 tctttgttac gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga
     1921 cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat
     1981 ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa
     2041 gggataaata ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc
     2101 tcaaagtttc taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga
     2161 tttctttcag ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc
     2221 ctcgcccttg ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta
     2281 atgcttcttc attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat
     2341 atctattttg ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt
     2401 tacccatttt tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg
     2461 gtttgttttg tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac
     2521 gtcgtaacat cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa
     2581 taaagttctt cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct
     2641 tgtgcattcg cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct
     2701 ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc
     2761 aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt
     2821 gcggtgtatc ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc
     2881 ggacaaaatc acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt
     2941 catctaataa ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata
     3001 aagcttctcc ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca
     3061 gccacttagc aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca
     3121 tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg
     3181 ccaaattctc tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc
     3241 gttgtaaatt ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt
     3301 attgtgtggt cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat
     3361 gtacagtgat gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta
     3421 catagccatt gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg
     3481 cgatttcatt ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg
     3541 tttctcctaa atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt
     3601 tgccccatgg tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat
     3661 ctaatgggtc ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca
     3721 gttcatcgat ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat
     3781 acacatcacc tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta
     3841 aaatatctgg catatgatcc atgactcttg gatgcagtgt agcaggctcg acattcaacg
     3901 ctttcgtatc ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac
     3961 ctaattcgtt tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat
     4021 cgactttata gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac
     4081 gagcatttcc gatatggata taattataca cagtcggtcc gcagacatac ataccgacat
     4141 gtcccttttg tatagggatg aattcttctt tttttctggt caatgtattg tagattttta
     4201 tcattctgat ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac
     4261 cgctgtagcg tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt
     4321 ttttccgatc gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt
     4381 aacagttggg tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa
     4441 taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc
     4501 gatgaacacg ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg
     4561 ccagaattga gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga
     4621 gaaacgatgc caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga
     4681 acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac
     4741 tttccactcc tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc
     4801 gccttttaaa taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat
     4861 aatcaatgat tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct
     4921 ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc
     4981 aaccattcat tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact
     5041 tttttctgtt catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg
     5101 accaagcaat tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac
     5161 acggattggc atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat
     5221 ggcagctttg acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc
     5281 gtggagaaca gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac
     5341 aggatgttca ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg
     5401 aggttgatac aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc
     5461 tgctacacgt ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc
     5521 cattgctttg atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg
     5581 tgatttactt aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc
     5641 gtcttcgccc acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag
     5701 atagcctaat tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag
     5761 ttttttacct gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca
     5821 gccaaatgct tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg
     5881 taaaacgtga gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg
     5941 cataccatcg cgtttttgta tgacaaagtc gccaccgata ttatcggatt cgaacgaaat
     6001 agcgcctttc accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac
     6061 aacagattct agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca
     6121 tgttcctgcg taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa
     6181 ttcttcttct gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag
     6241 cggttgatag atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc
     6301 aggagattca tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc
     6361 atcttcgata ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt
     6421 gtggcgggca aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc
     6481 agttggactt ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac
     6541 tagactttga ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta
     6601 aagccactca gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat
     6661 ttccttacca gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct
     6721 cttttttatt gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag
     6781 cagctgtttg aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc
     6841 catcttctac gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg
     6901 tcccggcttt tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg
     6961 catttgccaa cgcattgata ttaagaacag gtacattttg gaattcagag actttattca
     7021 agttatagtc attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc
     7081 tgtctacttc actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt
     7141 ctttttgcaa ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat
     7201 cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc
     7261 cttctaagaa tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat
     7321 ccaagatttt atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa
     7381 cttgactagc tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt
     7441 cttcgattcg tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa
     7501 tcggcaatac actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa
     7561 cacctaaagt caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga
     7621 cctcacttag tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca
     7681 aagataataa aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc
     7741 atccgtttga ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc
     7801 ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt
     7861 ttcgacaatc atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta
     7921 ctccaactat ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt
     7981 atacttttgt gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac
     8041 gtctgatttc tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt
     8101 tttctttata gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta
     8161 attttacccc acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc
     8221 ttttttccag cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc
     8281 gtttggcatt tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac
     8341 gtgtcccttc cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta
     8401 agaaaatctg tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc
     8461 caatctcatt cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat
     8521 gttgttcacc ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg
     8581 cgatcgagcc ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag
     8641 cgattttgag tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg
     8701 tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat
     8761 gttcgatggc acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt
     8821 tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata
     8881 catataagac ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg
     8941 atttcccgat acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc
     9001 ctaacacacg gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga
     9061 cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt
     9121 ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc
     9181 gtcccaagaa cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt
     9241 tttttgccat tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt
     9301 atctttaacg ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg
     9361 atcttgatct gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt
     9421 gatcactaca tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc
     9481 ctcattattg taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg
     9541 cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc
     9601 tttgattcca gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt
     9661 tttcaatcca tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg
     9721 aatcacgacc tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg
     9781 cggtagattg atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac
     9841 cggttttcct ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt
     9901 tctttacaaa ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt
     9961 tccaaacgac tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac
    10021 aagtagaata gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga
    10081 aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa
    10141 tggaaatatc aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa
    10201 tcggggaaag gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt
    10261 acatttgtca gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca
    10321 agccgaactg atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca
    10381 gatcctcgtg acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac
    10441 attggaacag cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc
    10501 agtggatgaa ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat
    10561 ggcaaaactt gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc
    10621 agataatccg actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga
    10681 aaagattctg gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag
    10741 taaattaaaa gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg
    10801 aaaaaatccg gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa
    10861 ggaggacttg aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa
    10921 caactagcac ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc
    10981 atcaaaaaga atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc
    11041 gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat
    11101 gccatggcta cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat
    11161 gagatgtgga cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg
    11221 accggcatca tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc
    11281 aaatcaggga atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta
    11341 ttagtccaat tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag
    11401 tttgtcgatg atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta
    11461 gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg
    11521 gtacctatca ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag
    11581 ttatctatgg cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac
    11641 gcggcagaaa cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa
    11701 aaagaactgg tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta
    11761 gagacggctg ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt
    11821 gctgtcaaag tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact
    11881 aaacattcag aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa
    11941 gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt
    12001 ggttttggtg cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg
    12061 ttaaatgaat taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa
    12121 tgtttcgtgt gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac
    12181 tagaaaggtg tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg
    12241 aatatcgctg cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga
    12301 agcataggga gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg
    12361 ctggttacac gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat
    12421 tactattctt ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt
    12481 tttcacttat cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa
    12541 aaaatatccg tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt
    12601 agaggctgtg acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac
    12661 cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg
    12721 ccatcataat aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct
    12781 tccgctgatt cagccagtag ctgttcccga tacacctgat acatacc
//
LOCUS       DACEZN010000074        12787 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEZN010000074 DACEZN010000000
VERSION     DACEZN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.074"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(137..1666)
                     /gene="ahpF"
                     /locus_tag="IXH60_001629"
     CDS             complement(137..1666)
                     /gene="ahpF"
                     /locus_tag="IXH60_001629"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1990552.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1676..2239)
                     /gene="ahpC"
                     /locus_tag="IXH60_001630"
     CDS             complement(1676..2239)
                     /gene="ahpC"
                     /locus_tag="IXH60_001630"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1990553.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2383..3045)
                     /locus_tag="IXH60_001631"
     CDS             complement(2383..3045)
                     /locus_tag="IXH60_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1990554.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3229..4875
                     /locus_tag="IXH60_001632"
     CDS             3229..4875
                     /locus_tag="IXH60_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1990555.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4866..5948
                     /gene="uxuA"
                     /locus_tag="IXH60_001633"
     CDS             4866..5948
                     /gene="uxuA"
                     /locus_tag="IXH60_001633"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1990556.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(6056..7609)
                     /locus_tag="IXH60_001634"
                     /pseudo
     CDS             complement(6056..7609)
                     /locus_tag="IXH60_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            7757..8898
                     /locus_tag="IXH60_001635"
                     /pseudo
     CDS             7757..8898
                     /locus_tag="IXH60_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(9024..10103)
                     /locus_tag="IXH60_001636"
     CDS             complement(9024..10103)
                     /locus_tag="IXH60_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1990557.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10206..10646)
                     /locus_tag="IXH60_001637"
     CDS             complement(10206..10646)
                     /locus_tag="IXH60_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1990558.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10703..11113)
                     /locus_tag="IXH60_001638"
     CDS             complement(10703..11113)
                     /locus_tag="IXH60_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1990559.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11293..11727)
                     /locus_tag="IXH60_001639"
     CDS             complement(11293..11727)
                     /locus_tag="IXH60_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1990560.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11827..12675)
                     /locus_tag="IXH60_001640"
     CDS             complement(11827..12675)
                     /locus_tag="IXH60_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1990561.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         4293 a         2366 c         2258 g         3870 t
ORIGIN      
        1 ttcaaattat ttactactta tagtatataa gaaaaagaga agaaaaagcg attattcaaa
       61 aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct tttttccgct ctctgacttt
      121 ttacaatctt catagctcaa gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag
      181 ctcctgaacc cattgaaaca atgatttgtt tgtatggact atctgtacaa tcaccagctg
      241 cgaaaacgcc ggggatattc gttgcgccat ttttctcggt cactatttca tttcttggat
      301 tcatttcgat cgttccatct agccaatccg tattagggag taagccgact agaatgaata
      361 ctcctgaaac agctagtgta tgcatctcca tcgtacggcg atcgatatag gagagagatt
      421 ctacctgatt cgtaccgctg atttctttcg tcgctgcatg tttgataaca gtgacattcg
      481 caagagagac tagtttatcc tgtaagattt gatctgcttt taattctggt aagaattcta
      541 gaacatatac atgttttgct aacccagcca aatcgattgc tgcttcaaca ccagaattac
      601 caccaccgat aacagcaatc tcttttcctt taaataaagg gccgtcacag tgtgggcaat
      661 aagcaattcc tttattttta aactcttttt cgcctggtac attgatagaa cgccagtgcg
      721 cacctgtcgc gatgatggcg gtttttgctt taagtgatat accgttagct aagccaatat
      781 taataaaatg ttgctcttta tttaaggaaa cggcctgttg attcatcata atgtcgatag
      841 ggtaactacg cacatgactt tctacttgct gcatcaattt tggaccttct gtgtaagagg
      901 taccaatcat attttcaatc cctagtgttt caacgacttg tccgccgaaa gaatcagcga
      961 caagtcctgt acggattcct ttacgtgctg catagatagc tgcactacta gctgctggtc
     1021 caccgccaat cactaataca tcataaggtt ctttttcaaa caactcttct tcaatcaaag
     1081 ggccagtgat tttttcaaca atttgttcga tcgtcatgcg tccgctacta aattcttctc
     1141 catttaaaaa cacagttggc actgccatga tttttttatc atcgatttct ttttggaaca
     1201 tcccaccttc aatcatcgta tgagtaatat ttggatttaa tacagctaat atattcaatg
     1261 cctgtacaac gtcggggcaa ttatgacaag tcaagctaac ataactgtca aaatgcaatg
     1321 tttgtgttat ttttttgatt ttaagactca aagattcact gattttcggt gcccgtccac
     1381 taatttgtaa catagccagt aaaaaagagg aaaattcatg accgaggggg ataccagcaa
     1441 aaacgattcc actttctttg ccaactggat tgatagaaaa actaggtgtg cgcatcgaat
     1501 ctcttttttc ccaagagatt ttaggagaca tttctgctat ttccttcgta aattcagcta
     1561 gtttttgact attttcacta ttatccaaac taacagaaaa aacaatagga gattcaatca
     1621 gtgctaaata ttgttcgaga ttttgtttag tttctttgtc taacatatcc accacctaaa
     1681 tctttccaac aagatcaaaa cttggtttca atgtttcgcc actttctttc cattttgccg
     1741 gacaaacttc acctgggtgg gtacgtacat attgagcagc gcgaatctta tcaatcagtg
     1801 cactagcgtc acgaccgatt ccatctgcat tgatttccat agcttgaacg attccgtctg
     1861 gatcaataat aaatgttcct cgctgagcta atccttgctc ttcatccaaa acatcaaaaa
     1921 tacgagaaat ctgatgagac ggatcaccga tcatcacgta ttcgatttta ccgatagcat
     1981 ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt
     2041 caacacctaa agatttcaat gtactgtatt gttcttgtaa gtcttctaat tcagtaggac
     2101 aaacaaaagt aaaatcagct ggatagaagc aaagaatact ccagttcccc ataagatctt
     2161 tttcactgac ttctaaaaat tctccttgat gataagcatg tgcattaaat tcttcgattt
     2221 ttttcccaat caatgacata gtaaaacctc caataataat tatttattta taattattgt
     2281 aatttagttg gagttgcaaa gtcaatggat attttgttat ttaataaaga ttactcaaaa
     2341 acgaaggtta attataaaaa tagaagaggt cgcaagaaaa tgctactgct ggaaatactc
     2401 gggataacgt tgagtaacag tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt
     2461 ttgaaatcga gcttcttctg gttttttatc aactaaagcc ttgagtaaat cataatgttg
     2521 ttccatgatc gtttcccatg gaagcccctg aatggtcact cgtaaccagc ggaaacgttc
     2581 taaatgggta ttatggtcat ctaaccagtt ccaaacttct tttcttcctg caatttcgaa
     2641 acaagcttca tgaaataaat tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac
     2701 tgctttttct tgttcttcta aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt
     2761 ggaacaacat tcaatcatga tttgctcttc caactgttct cggacaaagc gagcattttc
     2821 tgcagaaaca agattaattt tggaaacata cgtaccactt tgaggtatcg tatacacgag
     2881 ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga gtccggccga tagcaagatg
     2941 ttcctccagt gccttttcag atattttcct tccaggttct aagtcagcat agataatttg
     3001 tttacgaatc gcttgataag cttgtgattg caagctgctt ttcatcttta ttcctccaaa
     3061 atcatttcta tgaacgtagt ataacagaag aggtctgaaa aacaatctgg tatgtcacat
     3121 taactagtaa atttgtataa atagtaaaat tttggttgac atagcgattt ttattgcata
     3181 aaattcactt gaatacaaaa actaatatgt tagtttagga ggggaaaaat gattgattta
     3241 aacaactgga ggaactttat ccctgaatta tctcaaaaag aagtagaaat acctcatttt
     3301 gaccgtgcat cattaagaga aaatggacaa aaaaatcctg tctggatcca tttcggtggc
     3361 ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa
     3421 ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag aaataattga aagggcttac
     3481 caagattttg atgatttgat tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa
     3541 cgtatattag ctgcgacagc tgacagtctg tattgtcatc cagattgttc tgaacacttt
     3601 gataagatga ttcgacactt tgaaaatcct agtttacaat tagttacttt tgctattact
     3661 gaaaaaggtt atcgattgaa agatacaaat aataaatata taggaccgat tgcagtcgat
     3721 ataaaaaatg gtccaaagaa gccatcacat acaatgagta ttcttgctgt gctactttat
     3781 aaacgttttt tatctggagc ttggccgatt gcacttgtct ctacagataa tttttcacaa
     3841 aacggtagtc aattacgtac ttctttgcta acgatcgcaa aagaatggca aaaggaaggt
     3901 tatgtacaaa atacatttat ccaatactta tcgaatgaag aaattgttgc gtttccatgg
     3961 acgatgattg atcgtatcac cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa
     4021 gagggctttt caaatatgtc gattttccaa accgcaaaag gaaataatat tgctccattt
     4081 gtcaatacag aggacatcca ttatttaatc atggaagatc attttcccaa tggacgtccg
     4141 aaattcgaaa aagctggggt attcttgact gatcgtgcaa cggtagataa agcagatacg
     4201 atgaaggtga cgacttgcct taatcctttg catacgtctt tagctattat gggttgttta
     4261 ctggggtata ggagcatagc tgaggagatg aaagatccag atttatcgct gctagtttct
     4321 gaaatcgggt acaatgaagg cctcccagtt gtggagtctc caggtatcat tgatccaaag
     4381 atctttctgg aagaagtaat tactgtgcgc ttttctaatc caaatattcc taatatgcct
     4441 cagcgaatcg ccacggatac ctctcaaaag gtggcgattc gttatggtga gacgattaaa
     4501 aaatatatgg aaatagaagt taaagacccg cagacacttg tattcatacc attgactctc
     4561 gctgcttggc ttcgatacct tattggcatt gatgatcaag gaaaaccatt catcccgagt
     4621 ccagacccac tattgacaga ccttcagcat aagctttccg atattcaact gggtacgcaa
     4681 gatacatcac atattcatga gaaaatggct cctattttgt caaataaaga aatttttggt
     4741 gtagatcttt accaagcaga gcttggcaga aaaatagaag aaatgttcat caagatggta
     4801 gcaggaccaa atgccgtacg aaaaacatta caggattact tacaagaaca tggaggaaat
     4861 ttatcatgga aatgactttt agatggtacg gatcacagga agaaaagatt accttagatg
     4921 aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac attgatggat ataccagtag
     4981 gagaggtatg gcctagggag aggatcaaag aactaaaaga agaaattgag tctcgtgggt
     5041 taacactaaa agtaattgaa agcgtcaatg tgcatgatgc cattaaaatt ggattacctt
     5101 cccgtgagca gtatatcgaa aactataagc agacaatccg aaacttggca gaatatggaa
     5161 tagaagtcat ctgctataat tttatgcctg tatttgactg ggtgaaatct gaccttgact
     5221 accgcttaga agacggctca tccacgttag cttttatatc tgatgatatt ccagatgatc
     5281 caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga atttggattg ccgggatggg
     5341 aacctgagcg cttagctcct atcaaatcct tgcttgaagc ctatgcatca gtagacgaag
     5401 aaaaacttcg agaaaatttt gcctattttc tccaatcaat catcccaaca tgtgaagagg
     5461 taggggtcaa aatggctgta catccagatg atccgccata ctcgattttt ggattagctc
     5521 gaatcgtaaa gaatcgagaa gatttagatt ggttgtgtcg catagtagac agcccttata
     5581 acggaatcac tctttgtaca gggagtattg cagaagatcc agaaaatgat gtatatggga
     5641 ttttagcaga attttgccaa agagatcgaa tcccatttgc acatgttcga aatattaaat
     5701 ttttagctaa tcgagatttc tatgaggcac cacataaatc cgaatacggt tcattagaca
     5761 tgtaccgcat actaaaaaca atgtatgaca acggatttaa cggatacatc cgtcctgatc
     5821 atggaagaat gatctggaat gagacaggaa gaccaggata cggcttatat gatcgagcat
     5881 taggtgctgt ttatctaaat ggattatggg aagcaatcga aaaaggaaac aaaagtaaag
     5941 ataattagtg aaaaaaactg taattttatt atgtaaataa gaaggatgtt caaattcttg
     6001 atacataaat ccggaggttg ttaataagca ttcggattta tgtatcttat ttggtctagg
     6061 aagtaacttg attttctttt ttcgattgat ccatgagtgc ttcttctttt aattcaaaga
     6121 tatattctga gatcctcata atcacttttt tccagtcttc tggtgtgatg attcctttta
     6181 aaatatagat ttttttgata tgagaaggca attgtttcaa agggcagtcg ctgataacga
     6241 tgttgcagtt ctccataaaa tcattttgta ttttgatcga ttcatttgaa aaaatttgtt
     6301 ctatcttaac gatcagatct ttactgatat aaaaatcacg cgtatagttc acaccaatca
     6361 caattcgcgg ctgttttttg tagcgatcat acaaatgtac taggtcttca accatccatt
     6421 gcacattttc cttttctaaa ataactggta acaagtgttg attttgttct ctgaaatcca
     6481 tataaaattg ttcaatggcg gtttggactt ctttcaatac gctatgttct tgataaagca
     6541 tgaaagtagg aaggggcgta tttggttgat tgaagttaag aaatccatta taaagtttat
     6601 ttcgtgcgaa tgccaatgag taaagaatct tttctttttc aggtataaac aaatcaaata
     6661 cagtacaaag ggactccacc atttctcttg aataaagaac atgaggaacg tttgcttttt
     6721 ttaaaagttt gtattgctcc aagcttgttt cttccgattc gattttcgtg atagaaagtc
     6781 gagctaaaat attcactaag atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa
     6841 tatctacaga gagatcaata aaaaagtcaa tatattcata ctctgtgaat tctttttgta
     6901 cgtctttcac agaagttgca ggaaaattct ctttacatat ttttaataga aaatataatt
     6961 tatctatcat accgctattt agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca
     7021 taaaagccac tacttgcgga tcttcatcat aaaattctgt agcagtactt gaaaagatgt
     7081 tccaataaat atctaatgct gcatattgaa tttgtacctc ttttccagaa aaagactttc
     7141 tgccattatt agaaaaggag atagacaaac cgtactatgc taaaaatgca ttaatctttt
     7201 tcattttcgt atacaaataa gaggcactat agtttgtttg ctgactgaaa ctgataatcg
     7261 aatttgtctg acagctgaac aaagaagtca agatctgata actgactgat tcgttgcagt
     7321 atcttccaaa tagacgataa tagcagtaag ccggatcaat tccttcaaaa ttcaacttta
     7381 aaaaaggttt ttgcttatca atatgcagtt ctgttagatt gagacgctca aagtcttgtt
     7441 ctaattcttt gatgtatagt aaaaaatttg attcagacat atggaaatga tctgctaaat
     7501 attgtgtagt aataaagaaa cgatgttcag ataggaaaag aaaaatatct aattttgtat
     7561 tgcttttttt cttcaagata aacgttttaa tcgcttcttg agtttgcatg aaagaggctc
     7621 ctttttactt aaattgttaa aacttgaaaa tattactgtt tttttcataa atttagacaa
     7681 aaaaacagta aacatatagg tagaatctct actatcttat ttcttgcata caaaaaaatc
     7741 aaggagtgag agagttatga gatattcaac catcttaata tcaacatttg cagccttaag
     7801 cttatcgctt actataaatc agacagtcca cacagaggag acagaaacag ccaaatggac
     7861 agaaaccaag tgggccaacg tcaatcgaat tacctttcag gatgaatttt catccttcaa
     7921 ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc atcaaagcaa aaattgacta
     7981 ttcgggagac ttagcaccta tactacaatc agctgttaca tttgagaatg ttgatcccac
     8041 tttgtcgatt ggctatgacg agaacacttt gactatccaa cacaagcaag cgatatttac
     8101 cttcaccgtt acattaaatc aggacttgtt aaaaccagcg ctttttactg tcaaggtatc
     8161 agatggacta ccgaacgatc atcatttaac gccgtatagt cagcggcaga caattgagca
     8221 agattcagcc atcgactcag gaggagatct tgttgaagaa ccaacagata aaccagaaaa
     8281 tgaaaataag ccagaagtac cgcctacaga aaatccagac ggagaacaga aaccggaaat
     8341 agaacctgga gaagaacccg atacagaaac tcaaccagag ccagacaata aatcaaaacc
     8401 tgaaataacc cctggagaaa aaccagatgt aactccagaa ccagatacgg attctggaaa
     8461 ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc
     8521 agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga
     8581 ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag
     8641 taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg
     8701 aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc
     8761 tgtcctgaaa cgaaaaataa acagctacca aaatccggag agtcacaaaa caaagttata
     8821 ctgtggagcg gaatcattct attaagtata gcaacaatgc tttctgcaaa aagatttaaa
     8881 caaaacagat ctctctaaca tttgaataat gtctgtgaac gcatttttca aataaaaaga
     8941 aatatccaga aaatgacccc aaaataccag attgaaatct gatatttggg gtcgttcata
     9001 ctttatcttt cttcatattt attttaaggg atatctgttt cttttggaag atggttcggg
     9061 atctttttca ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag
     9121 ccccaaacgc caaagaaccg ttcgctaaca agtaagacca caaatgtata aaaaatcggc
     9181 agttctgttt tgctggacat caacttcgga ttcagcacat aagactcaat tagatggaca
     9241 atcaaaatcg taatcaggat ataaacaaca tctcggatgc ctccttggga ataagctatg
     9301 aaagatagag gaatacaaga aatgataaca cctgctactg ggatcagact taaaatgaaa
     9361 atcatgatcg ctaaactcag cagctgatga aagccaaata cagccaaggc gattgtagtg
     9421 agcaacgtat ttaaaagagc gacaacaaat tgagcttcta ataccaaacc aaatgtattg
     9481 acaaacttat cggcaaaata gtaaatatct tgaaagaacc aagaaaattc actttttaaa
     9541 aacaatcttg agaatacaat tgtttttttc ttttcaatca taaaaaagaa acttaagatg
     9601 aatgacatag caaacgccac tgccattttt cctatatcct gtaaatagcc aagcgcaatc
     9661 gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt atggatatat
     9721 tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa aattaataac ggactggatc
     9781 atgtcaaatg tttgatgaat aagcatcggg atatactttg tgaatgccag atagataagg
     9841 taaacgatca gtgtataaat catgatagct aaaacacctg tagggattcg gacaaatcgc
     9901 tgaatgattt gaacaagatg aatcatcaaa taagtaaaga taaatgtgag caaaatggtt
     9961 gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa ataataaaac agtgagacgg
    10021 cgtatttttt cattcgccaa aaaacgctga taaacggtca aatcgacaac ctccaaataa
    10081 caaaaaataa acacgttatt cattatatga tggaatatga aaataagaaa gagcaaaaag
    10141 aaaaaccgct aaatgacgtt atcttatgag ttggttttcc tcataaaatc gttcatctag
    10201 cagtttcagc cctttttctg gtcttgatga aaattatcta gcatttttcg ggtcagatgt
    10261 tgcagtgtat ccaattcttc ctgttcaagt ggaagataag acttgatctt ttgtggaata
    10321 ccttctgctt gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt tacgattctt
    10381 tcatcacccg tttcacgctg acgattaata taattttttt ctgctaactt ttttagtaag
    10441 ggagtcagcg taccagaatc cagacacaaa tcatttccta gctctttcac tgaagtcttt
    10501 ttcttttccc ataaaactaa catcacaaga tactgggtat aggtaagatt cagcggcttg
    10561 aggaggggag tgtagtgtct aatgatttct ttagaggcgg catataaagg aaaacatact
    10621 tgattaacta aatatagatc ttccattttt ccacctccgc tgttttacta tagtgtatca
    10681 tagcggattt acacaaaaaa gtctattcac taactgcttt cagagtaact tctatatttc
    10741 cagctgtagc ttttgaatac ggacacactt tatgcgctgc ctctaataat tcttgcgttt
    10801 cttccagaga aagaccttcg atagatcctt caatctctac acctaagaca aagctcgttt
    10861 cactttctga ataaagggaa acttgtgcac tgacagtaga agccccagag atattttttt
    10921 gctgtttgat caattctaat gcactgttaa aacatgcact gaaaccagct gcaaacaatt
    10981 gttcagggtt ggttgcattt tcgacgcgtt taccaggagc tgcaatattc aattcgaatg
    11041 tacgatcggg attatgcact tctccagagc gtccgcctgt gttgatcatt ttcgtagaat
    11101 acattttttt cataaaatta ccacctttaa ttatatttta tacaattgaa ttttacacaa
    11161 tataattgtg gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa
    11221 acagacgcgc tttctcgaat agcaaacata tacaaaaaat atcagagaca aaaaataatc
    11281 tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa aaattacttc
    11341 acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca ttgagttagt
    11401 cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat ttcgtaatgc
    11461 tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg tatttggatg
    11521 ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat ggaatctcgt
    11581 accatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg cctcaatcag
    11641 ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat catttaatac
    11701 aaaatcgatt tccgttcctt tatgcataaa gatctcctct ccttttcagt cgaacagtta
    11761 ctagattctt ttattatacc agctaaagaa aacgcattca aaaaaatagc ggatttactt
    11821 accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg ctacgctgct
    11881 gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc gggaaaattc
    11941 atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc cgccttttgc
    12001 ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga tgtcttccaa
    12061 ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt cgatataagt
    12121 catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt ctattccaaa
    12181 aataaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt
    12241 caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc tagaagagtt
    12301 gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa
    12361 caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag gaaaaaccaa
    12421 gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag ctagtaccaa
    12481 cataacgata ttcaacacta aaataactat agctcggtca aagcctaaag cagcttccag
    12541 taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta aaaacatatt
    12601 gatagcgact cctaatatga agagggcaaa aataatctta atgtatgtcc atataaattg
    12661 tctcgtttct ttcatttatt cacttcctta attcattaat aatactctta tcttattata
    12721 acttactttt caccttaagt taactttaat taataaaaaa attaggctag tctaaattat
    12781 tatgttt
//
LOCUS       DACEZN010000075        12648 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEZN010000075 DACEZN010000000
VERSION     DACEZN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.075"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..364)
                     /locus_tag="IXH60_001641"
     CDS             complement(<1..364)
                     /locus_tag="IXH60_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1990562.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIE"
     gene            495..998
                     /locus_tag="IXH60_001642"
     CDS             495..998
                     /locus_tag="IXH60_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1990563.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            958..1296
                     /locus_tag="IXH60_001643"
     CDS             958..1296
                     /locus_tag="IXH60_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1990564.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            1317..2735
                     /gene="ffh"
                     /locus_tag="IXH60_001644"
     CDS             1317..2735
                     /gene="ffh"
                     /locus_tag="IXH60_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1990565.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            2847..3425
                     /locus_tag="IXH60_001645"
     CDS             2847..3425
                     /locus_tag="IXH60_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1990566.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            3429..3950
                     /locus_tag="IXH60_001646"
     CDS             3429..3950
                     /locus_tag="IXH60_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1990567.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(4029..4583)
                     /locus_tag="IXH60_001647"
     CDS             complement(4029..4583)
                     /locus_tag="IXH60_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1990568.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            4836..5111
                     /gene="rpsP"
                     /locus_tag="IXH60_001648"
     CDS             4836..5111
                     /gene="rpsP"
                     /locus_tag="IXH60_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1990569.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            5123..5374
                     /locus_tag="IXH60_001649"
     CDS             5123..5374
                     /locus_tag="IXH60_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1990570.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            5499..6290
                     /locus_tag="IXH60_001650"
     CDS             5499..6290
                     /locus_tag="IXH60_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990571.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITRQDPMSALKMAVSMKRL"
     gene            6460..6981
                     /gene="rimM"
                     /locus_tag="IXH60_001651"
     CDS             6460..6981
                     /gene="rimM"
                     /locus_tag="IXH60_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1990572.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            6971..7732
                     /gene="trmD"
                     /locus_tag="IXH60_001652"
     CDS             6971..7732
                     /gene="trmD"
                     /locus_tag="IXH60_001652"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1990573.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            7868..8215
                     /gene="rplS"
                     /locus_tag="IXH60_001653"
     CDS             7868..8215
                     /gene="rplS"
                     /locus_tag="IXH60_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1990574.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            8588..9250
                     /locus_tag="IXH60_001654"
     CDS             8588..9250
                     /locus_tag="IXH60_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990575.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            9329..12556
                     /gene="fss3"
                     /locus_tag="IXH60_001655"
     CDS             9329..12556
                     /gene="fss3"
                     /locus_tag="IXH60_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1990576.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         4457 a         2255 c         2619 g         3317 t
ORIGIN      
        1 gttcaatccg tctgaatatt gctttcatac gggctaatac ttcgcgagga ctaaatggtt
       61 tggtcaaata gtcgtcagca cctatttcta ggccaatgat ccgatccact tggtcatctt
      121 tagccgtcag catcaaaatc ggtgtttcat ttttttcttg acgcagccgc tgagtgatct
      181 ccatgccatc catcgttgga agcatcacat ctaaaatgat gaaatcaaat gattgttcta
      241 atgctaattc taatccttcg cctccatctg tggcactggt taccttgtag ccttcttttt
      301 ccaaattgaa cgtcaagagc gttacaatag aaggttcatc atcaacaact agtatttttt
      361 tcattcaaat ctcctttaaa taggtttcat tctgtttata ttttagcata gaatcagtac
      421 aaagggctat tttacaaaag aaataaatgg tataatagga aggcactttg ctgaatagaa
      481 aggaagagca gtcaatgatt ggaatcagtg catgtttagg cggtctggcc tgtcgctatg
      541 atggacagaa aaaagaagta ccagaattga tggaactagt tgaaactgga gaagctgtca
      601 tggtctgtcc agaagtgatc ggcggattac caattcctag agatcccgct gaaatcattg
      661 gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt atggacgaca agcggcgaag
      721 atgtaacgga agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg caggaaaaac
      781 acattgatct agtcatcatg aaggaaaaaa gcccttcatg cggaacccat tcgatttatg
      841 acggaagttt ttccggaata aaaaaaaatg gtgccggagt ggctactgct tattttattc
      901 agcaaaaaat gactgtgctc tctgatgaag aatggctgaa aactagaggt gagcagaatg
      961 gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat tttattcgac cttattgaca
     1021 gaaaaacaaa tgaactacat ggaattatat tatgcagatg atttttcatt aggtgagatc
     1081 gcggaagaat acgaagtaag ccgacaagca gtatatgaca atatcaagcg gacaagcaag
     1141 atcttggagg attatgaaaa aaaactccat cttttttcta attatattgt tcgagaacag
     1201 gtattggaaa atatgactgc atatattaca gataagtacc cggaagataa aaaactactt
     1261 gaatatgtac agcaaattca agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg
     1321 catttgaaag cttaactgaa cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa
     1381 aagtatcaga agctgacgta aaagaaatga tgcgggaaat ccgccttgcc ttactagaag
     1441 ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag
     1501 gggcagaagt gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag
     1561 aattgacgat cacacttggt tctgaaacag cagagttaaa taaatcacca aaaataccaa
     1621 ctgttatcat gatggctggt cttcaagggg caggtaaaac gacctttact ggtaaattag
     1681 ccaattattt aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc
     1741 gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt cctgtatttg
     1801 atatgggaac tgaagtcagc ccggtagaga tcgtccgtca agggatggag ttagcaaaag
     1861 aaaagaaaaa cgactatgta ttgatcgata cggcaggacg tctgcatatc gatgaaacat
     1921 tgatggatga gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg
     1981 tcgatgccat gactggtcaa gatgccgtca acgtagctga tagcttcaac cagcaattag
     2041 gtattacagg ggtcgtcatc actaaacttg atggggatac tcgaggcggg gcagcactat
     2101 cgatccgctc agtgacaggc gcaccaatca aattcatcgg ttctggtgaa aaattgactg
     2161 acttagaagt attccaccca gatcgtatgg ctagccgtat ccttggtatg ggggatatgc
     2221 tgacattgat tgaaaaagcg cagcaagact acgacgagaa aaaagcggaa gaacttgcac
     2281 agaaaatgcg ggaaaattca ttcgatttca atgactttat tgaacagctt gatcaagtga
     2341 tgggtatggg cccactggaa gatctaatca aaatgatccc aggaatgaat caagtccctg
     2401 gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg atggtttatt
     2461 ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa
     2521 ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa caattcaagg
     2581 aatcaagaaa aatgatgcag caaatgtcca aaggggatat gaacatccca ggtatggacc
     2641 aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca
     2701 agaaaaataa gaagaaaaag aaaaaacgga aataagatga aaaaaacagc ttcggtacag
     2761 actgaaaatg tctactgccg gagcttttct atcagcaatt tttattcttt actaatacaa
     2821 ttactctaaa cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa acagaaagta
     2881 tgcggcaata tcatgatact gtctggggtg ttcctgaata tgatgaccaa aagctgttcc
     2941 gaaaattgat gcttgatatt aatcaagccg gattgagctg gcagacgatt ttaaataaaa
     3001 gtgaagcatt cgatgaagca tatgatcgat tcgatatcaa aaaagtcgca aattatggag
     3061 aaacaaaaat cagtgaacta atggaaaaca aaggaattat tcgtaaccga agaaaaatcg
     3121 aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc ggcagttttt
     3181 cagagtatct ttggtcgttt tctaatggaa aaatcgttga ttcttcttat cattcacaag
     3241 aagaagtccc aacaacaagt cctctttcag atgaattagc aaaagatcta aagaaaagag
     3301 gatttaaatt tatcggaagc acaacaatct atgctttttt agaagctgtc gggatcatca
     3361 atgatcacct tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg
     3421 aataaagcat gcgaaaaatc atattttatg gtgctataag tttagatggc tatttggcaa
     3481 cgaaaacgga tgatcttcaa tggctttttg acactccaac aggtaagcaa actacttatg
     3541 aagcttttta tgaaacaatc gatacgacca tcatgggcag aaaaacttat caggaagcaa
     3601 aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc
     3661 acgcacaatt acaattgcca gatgctgagg tcgtaaacga agatcctgtt gcttttctaa
     3721 aaaatctaca aatgacagaa ggaaaagata tctgggttgt gggtggcggt cgattgatca
     3781 aaccgctatt ggaacatcaa atgattgatg aatggtatat ccagattacc cctgtattat
     3841 taggggacgg gattccttta ttccaaaaag gaacatatga agcgcgtttt gaaatgctag
     3901 acacgacacg attcggtgaa tttatcgaat tgcattatgt acgaaagtag aagtaaataa
     3961 aataaaaaag gcaactatat gcagaaatgt cctgtacata gttgcctttt ttattaccgt
     4021 tatgcttttt actctgttaa tttgttagaa aaatctagat acgcatctaa aatcgtttgg
     4081 aagaaactta atgttccttc aacgatatta ccattttcat caattagatt cgcaacgttt
     4141 ccgatatatg cttccggttg ctgcaaggtt ggcacattca aaaagacaag tgattggcgt
     4201 agatggtggt ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac gactaaacct
     4261 ggtttatgat cccaaacact gtgtccataa ggtcttgagc ctacatctaa agcatttttc
     4321 aatactgctg gcacagaacg gttgtattca ggagtaacga aaacgacacc gtctagtccc
     4381 ctcacttcct cacgaaaacg agtccattca gcaggagcct gttctggagt ttcgagatcc
     4441 tcattgtaaa atggcagatc atcgattttt acaaatacac tttcgtaccc ttcaggaagc
     4501 aatttcgcaa aagcttctgc tacttttttg ttgtaagaat cttttcttaa actaccaata
     4561 aaaaatccaa ttttttttgt catacgattt ttcctccttc aaactatcta taataatctt
     4621 acaaaagata agtaactgga gcaaataaaa acacttactg acaaaagcta catagaagat
     4681 agattactta tttttaacaa aaaagaagtc taaaatcttc tattttctaa aaaaaatcga
     4741 atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg ttacactaca
     4801 tcttgtgaat aaaacaatgg aggtggaaat aaataatggc agttaaaatc cgtttaaaac
     4861 gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt agctgattca cgttctcctc
     4921 gtgatggacg tttcatcgaa actgtaggta cttacaatcc tttgaaagac cctgcagaag
     4981 tagttttaaa agaagactta gttcttgact ggttatcaaa aggtgcacaa ccttctgaca
     5041 ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct
     5101 caaagaaata aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa caatcgttcg
     5161 tccgctagtc acttatcctg atcaagtcaa gttagacgtc gtagagtcga aagactttta
     5221 cgagtacaat ctaacagtgg ctcctgaaga tattggccgt atcattggaa aacaagggcg
     5281 tgttgctaaa gcaatccgca caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt
     5341 gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac gtgctgtact cgaattttcg
     5401 agtgcagctt ttttgttttt tttattaatt gaagaaatga ttatttggca aaacaagcaa
     5461 gaaagcgtat ccttatagga ggagggagtg aacagccaat gagaaagaaa atgttattta
     5521 ttccatttgc tctattgtta agcgcttgta gttcggttac tcaagaatca gacaatacac
     5581 atctatcaag cagtatatct gaaactgtta ccagtccaga aaaaagtagt accaaaacgg
     5641 cttccaccac tgaacaaaca atggctacct caaataatga gaaaaagact gctttagacc
     5701 agttgaaaga acaacagcca aatgttccca tgcctctaga tgtaccagtc tcttcaggct
     5761 atctcaatat cgcagcgaca catacaaagc aaggttattc tattttatat tacagaacag
     5821 atcgtcccct cgggttgaat gctgacgaat tgaatcaaga aacgcctatt gcaacctatc
     5881 tatatcaata tgggtttgct tctagtcaag aaacaatcca agttttgcag ccatttgaga
     5941 tcgacacaaa tggtcagcaa gtggacttag gttctcgaat cacagggtat cagcaaggtg
     6001 cagcaggttc aagttttctt gaatggcaag aaggaaactg gcgtattcga atccgtggga
     6061 ataatataga aggccaagat cccttactat tagctaaaga aatcgtagct tatttggaag
     6121 aaaacagtct accagcacct gaacaatttg gtaaaattac tgttgatatg ggtgatacga
     6181 cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa tgcctatacg atcactcgtc
     6241 aagatcctat gtctgcgttg aagatggcag tttctatgaa gaggttgtga aatcagcgcg
     6301 ctgatctgag cccatcattt aactccaagt cacaggaagt aatcaaaaat agcacgccgc
     6361 atttttgcat aattttggct taatacctta aaagaaaact agaattgcct cttcacacat
     6421 gctaaaataa aacagacaaa aaagaaatgg ggatataagt tgactgaata cttgaatgta
     6481 ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat
     6541 ttcccagaag aacgttacaa aaaaggaacg gtgctaacac tattccaaga cggaaaagca
     6601 cctgtggaat taacagtcaa aagtcatcgc aaacataaaa attttgactt attgagtttt
     6661 gaaggacatc cttctatcaa tgatgtggaa aaatatcggg atggcatatt aaaagtgtca
     6721 aaagacaact ctgtagatct agctgaaaat gaattctatt accatgagat catcggtctg
     6781 catgtattag aagatggaac tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc
     6841 aatgatgttt gggtcgttca acgtccgaaa aaagctgata tattgcttcc atatatcgct
     6901 tctgtcgtca aagaagtcga tttagaagct ggagtggtcc gtgtagaaat tccagaagga
     6961 ctgattgacg atgaagattg atgtattgac tttatttcca agaatgtttg aaggtcctat
     7021 gggggaatcc atcatcggta aggctgtaga taaaggatta ttagatatca atatctctaa
     7081 cttccgcgat tattcagata acaaacatca gacagtagat gattatccat acggcggtgg
     7141 agcaggtatg ctgctcaaag tccagcccat ctatgataac atccaagcca ttgaaaaagc
     7201 tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac gattcgatca
     7261 gaaaatggca gaagaatttt cacaagaaga acacttagta ttcatttgtg gtcattatga
     7321 gggatatgat gaacgtatcc gcacattagt gacagatgaa gtttccttag gagattatgt
     7381 ccttactggg ggagaattag gtgcgatggt catgatcgat gcaacagttc ggcttttgcc
     7441 agatgtgtta gggaatcaga cttcagctca aacggattct tattcaaccg gtttattgga
     7501 acatccccaa tatactcgcc cggcagaata taaagggatg aaagtaccag aagtattgac
     7561 aaatggaaat cataaattga ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta
     7621 tcaacgtcgg cctgatcttt tggaaacact tgaatggaca cctcaaatga cgaaattttt
     7681 agaagaaatc aagaaagaag aacaagaaaa aacagcagat gaagccaagt gactttttct
     7741 aaaatcttct ttacagtagg ttgataatat gttagtattt tatggtgagt gcaaaagcac
     7801 ttaactatta cgatattccg ctgtgagaaa agctcataag aatatttgga ataaggagaa
     7861 gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa caattacgtt ctgacatccc
     7921 agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga
     7981 acgtatccag ttatttgaag gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac
     8041 ttatacagta cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc cattgcatac
     8101 accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta
     8161 ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa gaaatcagaa gataaacacg
     8221 gaaaacttcc ttggaaatct gaaatttgtg aaaagtcctt gtatatcaag ggctttttct
     8281 tttttctcta tcacagtttt aagccatttt tggctgtttt ggaccatttt tcaatcatat
     8341 tcccttaaaa attgtcctag attgtccgac attgactatc agtgtcgtca aaatagggca
     8401 aaatttagta gtcagcttga cgacaaatat aatacgaaaa ataccaaaac atttaccgaa
     8461 tttagcaact tttgaaccca agtttctcaa agggtcagct attagcaaaa aagacaaaac
     8521 atgtactttt ctactaaaaa gttgatgtga taggcttcta tctaaataac tattaggagt
     8581 ttttatcgtg aagcaaaatg atggacgtat catttggaaa aatcaaagtg agttaaaact
     8641 gattttaaca attaatgaat tcatcgaaaa acacggaatt acatctagcc gtcaatacca
     8701 gaaaaagcta tcagaaaatc caaattctgc accaagtatg tggttcatta acaaaaaata
     8761 tggatcatgg gagaatttat tgattagtat aggacgagaa aatactgggt atggaaaatg
     8821 ggctagaatg tcagaacaag aattattgga aatagtagaa gcctttatta agtgcgagaa
     8881 gataacctct caacgaatgt acgaacaaaa atcagtaggg aagaatattc cttcacttag
     8941 taccattaaa aagatgttag gagatatacg tccacttttt aaagaaaaaa atgatggatc
     9001 tcgatttact gatttcgaac tgctgttaga actaaaaaat gaaataatcc gattaaaact
     9061 acaggatgat ttatcaatga caaagtttcg aaaactagtt caatccccaa aactaccatc
     9121 agttgataca attatgaaaa gaacgaacaa aaattgggag gaattgatgg cagagattgg
     9181 atttgattat cgaagaatca aaatttataa gcagagaaac aacctatcca agacaaagaa
     9241 aactaaatag tatcactcta aatcagacgt tcaaaattaa aatttggacg tcttttttat
     9301 tacccaaaaa cgaaaggaga tttattttat gaaaaagcca agatttaaga attggcgttt
     9361 gctcgcaaca ctgagtctat tatgccaaac aattggtgga tcgcttggcc caacgattgc
     9421 ctttgctgat gaaattactc atccacaaac ggtaactgtg gaattggatt tggctcacca
     9481 gtatgctgtc gaaggtacat ttagcgatgg tcgtcccatg tcagaagtta ccgttccgca
     9541 ctatgcagtt tacaatggtg tgaagcaaga tattttctgt attgagccag gggtgccgat
     9601 ttacaatgag ttcactcctg gttatgagaa aaatccattg cctgatatgt cggagaaagc
     9661 gaaattagtt tccgttttat ggaaggaagc tggtacggat gtggatacac atattgtggc
     9721 ccagaagatg atttggcaag aagttaatgg ctacacgctt cactcaatta aacgttctga
     9781 tggtagtgca gtaaatatcg cagccattga gtcaaaaatc aatcaagcca ttgcggatta
     9841 tcaaaagaaa ccaagcttcc ataactccac agtgaaaacc gtgttaggtc aatcgactac
     9901 tgtgacagat acgaacggct tgaatttatc ggagtttgat gaagttgtcg agaatatcgc
     9961 aaatattgat tatcgtgtga acggcaatca actagtgatt actccaaatg ccaattcaaa
    10021 agagagtggt gaattaaccc taaagaagtc agctaataca ggaacaccgg tagcttataa
    10081 aatggctggt caacaaactt tgatggcggg ggcaattgat aagcccaata cctacacaat
    10141 caaaattgat gtggaaactg agggttcttt gaagattaaa aaagtcgata aagaaacagg
    10201 aaatgttgta ccgggaaccg ttttccattt agactttgga aaaacattac ctgcaaaaga
    10261 cgtgaccacc gataaagaag gcattgcgac attggatgag attcctcatg gcgcgaaagt
    10321 aaccattact gaaaaatcag tgccagctcc ttatacgatt gacactacac caatcaccgc
    10381 aaccattaaa tctggtgaga ctatttctgt gacttcaaaa aatactcgcg aaaaaggtca
    10441 aatcattttg aataaaatgg gggtggaaac aggaactgat ctttggaacg acaaatactc
    10501 attagccgga aatacgtttg ccattcgcaa agacagtcca accggtgaaa ttgtccaaga
    10561 aatgaccacg gatgaaaaag gtcatgcgga aacaccaaaa gagattgcca atgcgttgga
    10621 attgggaacc tattatgtga ccgaaaccaa agctagtaat ggctttgtga atacctttaa
    10681 accagtaaaa gtcgaattga actatgtcaa tcaaaccgtg gctcttgtta ccagtaacgt
    10741 aaaagggcaa aaccaagaaa tcactggtga aaccactttg acaaaagaag acaaagatac
    10801 cggtaatgaa actcaaggta aagctgagtt taaaggagct gaatatactc tctttactgc
    10861 aaaagatggt caagcggtca aatggagtga ggcttttaaa ccagagttag tgaagggaac
    10921 gaaagcttct gatgaaacgg tgactttgac tttagatgaa aagaatcaag ttgctgttaa
    10981 acacttagcg attaatgagt attactggca agaaaccaaa gcacctgaag gatatacctt
    11041 ggatgaaaca aagtaccctg tttccatcaa aaaggtggat gataacgaaa aaaatgccgt
    11101 gattacccga gatgttacgg caaaagaaca agtcattcgc tttggctttg atttctttaa
    11161 atttgctgga tcagctgctg ggactgccga aactggcttc aatgatctga cctttaaagt
    11221 atcgccattg gaaggaacca gtgagattac agatgccgaa gatgaagcaa ttacagctta
    11281 taacgagcaa ctaggttttg atggctatgg caagtttgaa aatcttcctt atggggatta
    11341 tttacttgaa gaagtagagg ctccagaagg atttcaaaaa attacgccat tagaaatccg
    11401 ttctactttt aaagaaaata aagcggatta cactaagagt gaatacattt ttacgattac
    11461 cgaaaaagga caaaaacaac caattaaaac ggtgaccgtt ccttacgaga aactgacgaa
    11521 taaagcattt tctgttagct tgaatcgttt gatgctctat gatttacctg aagaagaaga
    11581 tagcttaact tctcttggga cttggaaaga cggaggcaaa gaattgacag accttgattc
    11641 taccgagcta gttgataaat tgagctataa cttgcatgaa atcaaagaag actggtatgt
    11701 ggtagcgcaa gccattgatg tggaagcgac aaaagctgcc caagaaaaag atgaaaaagc
    11761 caaaccagtg gtaattgccg aaacaaccgc aacgttggcg aataaagaga aaacaggaac
    11821 ttggaaaatt ttgcataaat taaccgcaga acaagttttg gataaaacca tcgtcttatt
    11881 caactatgtg tatgaaaaca agaaacccta tgaagcaggt gatgatccag tagcgaaaga
    11941 tgctagcttg aacaatcaag cccaaactgt caattgtacg gtggaacgcc atgtttccat
    12001 ccaaacaaaa gcccacctag aagatggttc gcaaaccttt actcatgggg atgtggtgga
    12061 tatgttcgat gatgtgtcga ttacccatga tgtactggat ggctcaaaag aagcttttga
    12121 aacaattctg tatgcattac ttccggataa tacgaccaaa gaaatttgga aatctggcaa
    12181 aattgattat gaagtgaatg acaaagaatt cactaaaacc gtacttgcgg aaaaagtgga
    12241 cacaagtaaa tatccagaag gtacaagttt taccttcaaa gaaattaact atggcaaaga
    12301 cggaaacatc aatgggaaac acaatgagga tctaaaagaa aaaactcaaa ctttaacacc
    12361 taaaaaagtt ccgacaacac caagtgcacc agaacaaccg gaaataccaa ctgttccaag
    12421 tgactctcaa gaatctagtc ctacagtaaa gacattccca caaacagggg agaaaaattc
    12481 taatgttctg ctgtttattg ggtttacctt gatctttgcg actgctgggt attatttctg
    12541 gaatcgccga aactaaggtg atgtgatgaa acaactaaat aggaaaaacc aacgtgcgcg
    12601 accgcctcct aaaaaaagtg gtcgcgctat ttttgtatcc aaaactaa
//
LOCUS       DACEZN010000076        12526 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEZN010000076 DACEZN010000000
VERSION     DACEZN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.076"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(192..491)
                     /locus_tag="IXH60_001656"
     CDS             complement(192..491)
                     /locus_tag="IXH60_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990577.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            complement(488..691)
                     /locus_tag="IXH60_001657"
     CDS             complement(488..691)
                     /locus_tag="IXH60_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1990578.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            complement(698..928)
                     /locus_tag="IXH60_001658"
     CDS             complement(698..928)
                     /locus_tag="IXH60_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990579.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            complement(925..1236)
                     /locus_tag="IXH60_001659"
     CDS             complement(925..1236)
                     /locus_tag="IXH60_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990580.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            complement(1375..2190)
                     /locus_tag="IXH60_001660"
     CDS             complement(1375..2190)
                     /locus_tag="IXH60_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1990581.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            complement(2187..2513)
                     /locus_tag="IXH60_001661"
     CDS             complement(2187..2513)
                     /locus_tag="IXH60_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990582.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            complement(2510..2671)
                     /locus_tag="IXH60_001662"
     CDS             complement(2510..2671)
                     /locus_tag="IXH60_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1990583.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(2686..3045)
                     /locus_tag="IXH60_001663"
     CDS             complement(2686..3045)
                     /locus_tag="IXH60_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1990584.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(3042..3893)
                     /locus_tag="IXH60_001664"
     CDS             complement(3042..3893)
                     /locus_tag="IXH60_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1990585.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            complement(3908..4708)
                     /locus_tag="IXH60_001665"
     CDS             complement(3908..4708)
                     /locus_tag="IXH60_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1990586.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(4751..5641)
                     /locus_tag="IXH60_001666"
     CDS             complement(4751..5641)
                     /locus_tag="IXH60_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1990587.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(5643..6584)
                     /locus_tag="IXH60_001667"
     CDS             complement(5643..6584)
                     /locus_tag="IXH60_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1990588.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEKGIK"
     gene            complement(6817..7152)
                     /locus_tag="IXH60_001668"
     CDS             complement(6817..7152)
                     /locus_tag="IXH60_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990589.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            7419..7643
                     /locus_tag="IXH60_001669"
     CDS             7419..7643
                     /locus_tag="IXH60_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1990590.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSPGNDPHPPKG"
     gene            complement(7952..8107)
                     /locus_tag="IXH60_001670"
     CDS             complement(7952..8107)
                     /locus_tag="IXH60_001670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990591.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            8179..8409
                     /locus_tag="IXH60_001671"
     CDS             8179..8409
                     /locus_tag="IXH60_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1990592.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            complement(8577..8843)
                     /locus_tag="IXH60_001672"
     CDS             complement(8577..8843)
                     /locus_tag="IXH60_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1990593.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            9043..9747
                     /locus_tag="IXH60_001673"
     CDS             9043..9747
                     /locus_tag="IXH60_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1990594.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            9834..11123
                     /locus_tag="IXH60_001674"
     CDS             9834..11123
                     /locus_tag="IXH60_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1990595.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            11189..>12526
                     /locus_tag="IXH60_001675"
     CDS             11189..>12526
                     /locus_tag="IXH60_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1990596.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITV"
BASE COUNT         3749 a         2454 c         2036 g         4287 t
ORIGIN      
        1 atcagtcgga gcgatccggc tttttttatt tgccgagtta cacttctcga cttacatagc
       61 tcacaacagc tgcatagtaa tttgcataac ctctcttaga agttgctaaa gatatatgct
      121 gaatctcatt gtttttcgca aaatcgttta attctttttc taatttataa cgagtgtcct
      181 cttcaaagat tttaaacttc attgtttata acctccatat ccaccaatct cgctacagct
      241 aaattctctt tgcttttcgc taaccactta tcgcattcca ttgtgttttc aatacgaatg
      301 atcgctgagt gattatagac gtgttctaca tatccacgaa acggataaat gaactcttct
      361 gcttcacagc gaaccatgtc accgactttg acttttggtt tcttacgtgt tttagggttc
      421 tttgtcggca tatctagcat taaaccgccg atgccgtgac tactagcgta aaatccgtct
      481 tttagtttca tctcatttcc tcccatttac gatcatcatt taatatcgaa atcccaaact
      541 tacgaatagc atcacttgca tcagcaacaa actgacttgc cactttatat gtttcttctg
      601 ctgaaattcc atattctttt tcaaactttg tctttagtac atttagttcc tgttttctta
      661 gttttgctac tctgcggtgt ctggtgttca ttgtcaatca actccctaat ctgaaagtgt
      721 tgtctatact tgatcgaaat tcttttaagt ggttctctac cacagaatca gtcacgttaa
      781 aacgatcaat taatactgga gctgccatat ctttcaaata actttgtctg atgactaatt
      841 cagtaccatc aggaagttct atgttaacct cccgaccatt gataattgct tgaatgcccg
      901 cttcacttag tggtatttcg tatttcattt cacatcctcc aaatcactcg acttcacgaa
      961 tacaccatct accattttcc ctgtgcgtcc tttgatttcg ttgtatgcca tttctaaaca
     1021 ctcttgtacg tttgtccctt tttgcatgga aaggataatc agcgtgacga taacgtctcc
     1081 tacgctatct ttaaatagtt catcattact tcttgccatc gccgaagcta tttccccgaa
     1141 ttcctcagct actttcaaaa actgtgcttt tggatctgct tgatccagtc ccttgtcttt
     1201 agcccactgc tctacttttg tgattagttc gtccattatt tctcctccta aaatttcatt
     1261 tcatcgttgt tgtcatcttc tttgtcatca gtttgggaaa ggagcgcata cacgagatat
     1321 gctgctccta ctaaacctaa gaacaagaga attttagcca taaagaatcc catattattt
     1381 gcctgcttca tccgtaacta cagtatctgc tccatttact gttacccatc catgcttttt
     1441 gcgagcttga gcttcttcat aacgaatcaa attgtctgtt actgattcgg cgactttgcg
     1501 attagattct gcctctgctt ctgcagcttt tgttttcttg taagcttcac tatcagcttg
     1561 agtttttgca gtttctgcgt ctagcttcgc tttttcattt tcttgaccag ctcgaatgat
     1621 cgcatcgatt gatttttgtg tttctttatc gacatctgga acaccaagtg ttacgtcttc
     1681 gacttcaaac cctttagatt caactgattt agcaaagttc gttaatacct cagcttcaac
     1741 tttagaggaa tctcctgaaa ggacatcaag cagactatat ttcgcataaa cttcacgagc
     1801 gaccttttga agcttagatt ttaaccatcc actttcgata tcttccgaag tgatattccc
     1861 aaattccttg tacatttttg ctgctttagt tgaatcaact ttgtagtcat atttgatatc
     1921 aatcgttgtc tttttgccgt cgcttgtaga tactgaaata ttttttgatt ggatagtttg
     1981 caagcgaatt ggatattgga tcactttgtc aatccctaca aatttcacac cttgcgtcag
     2041 tgcttcatct ttgataccgc cgttcattga atagcgcaca cccacatatc cattatcgat
     2101 tttttcgaag aacttaaatc ctccgataac tccaatacct acgattactg ctcctgctac
     2161 acccaatttg attaatttgt tttcgttcat ttttcttcct ccaaatactt atattcgtgc
     2221 ccttttcgat cggtgtgatt tcgccaagcg tacgttcgaa tagtcccacg cgtatatcca
     2281 gtttctgcag acagctcact agctgttcct tgcatcaaaa ttttgccttc gtgtaaaaca
     2341 acgacgattt tccctttgcg ctgttttctt ttgtctggct tttttcgtat acgtccgtta
     2401 gcaatctttc ctagacgctg aacttcagca accactgctt catcttcttg ccagttctca
     2461 tcttgaatca gcaacattaa ttttcgccaa gccgcttcct tatccacgct cattcctcca
     2521 atctacggat ttcccttctt aaattctcta tgtgcaaatc gattgccttt ctagccgttt
     2581 cattgaccat cactgccttt gttcgttcca gatcgtcaat ctcacgccga aggcttcgaa
     2641 ttcgcatttg aatcacttct tctgttgtca tgatggacca cctcgttaaa aacgctcttc
     2701 cttgaacgta ttccgatatt ttttggctaa tatcaacggc acttgatatt gatgacagaa
     2761 caactttgcc ttgatcttaa agtcttttgt ctgcatcccc ttaacatcta cgactttgac
     2821 cagcttgccg cttttgtaaa atgtgaagtc gggaatatac tcgatcttgc gatacttctt
     2881 gccctctagt tcaaacttcg gcatcagctc aaacctttcc tggagcttca ctttccaacc
     2941 attcgcttca gcttgccaca aggctagatc gtaatactct gcttccgcga tagaatcaaa
     3001 cttgatacct cgatgaacag tttttttatt acggtattta ttcatgcgat actacctttc
     3061 actggtttta tgcgcttgtc tgctgtttga gaaaatacca tcgtaaatcc atcagagttg
     3121 acaaacattc ttgatattgt tctcgtaccg taagcttttt tcagttcttc accaagcaag
     3181 ttcgttgtga taatcgttgc taggttctgt cgtgcatcca agaacgaatt tagcgtgttt
     3241 atgccaaacg ctcgactatc tgatgcatct tttcctagtt ctgaaccgat atcatcaatg
     3301 atgactaaat ccgctgtttt gatatctgca atcaacgatc cctcaatcgt ttttctgagt
     3361 tcaggattgt tgtacgaaaa tttgatttga tccagcattt cctgtaaccc gataaataag
     3421 atttttttat cgtagtttga tcgcttcaag acttcccaag ccgctgccat cgctaggtga
     3481 cttttacctg ttccttgttt tccagttaaa acaaggtgac ttggatttcc taacaagact
     3541 gaattaacaa agcgtttggt tacttcaacc gcttgtcttg tttcttgatc gacaatttga
     3601 taattctgca atgtgcaatc aaacaacgct tgattaggca caacagaacc acctttgaaa
     3661 aaattgattg ctcttgcttt caggctctcg ttgtaaattc gttctgtttg taagtcttcc
     3721 tgaactcgca atgctttata cccacattgc atacaggttg gcttacaacg ttctgagcca
     3781 tcagggttct tcgtacgcca tccatacaaa ggctgtccgc attcaggaca ttcgccacgt
     3841 tggacaagca ctttctgtat cagcttttcc atgatttccc caacagtttc catgcctacg
     3901 cctcctctca aataggcaac tcatctgtgc taaatttctc gtattcaagc gttttagttt
     3961 gttttgtttt ataatcacga tttgcttgcg ctgccattgt gtcgtatttt tctcgcaatt
     4021 ttttagcaga taaaatattc gatgcccaaa acacattgtg ctgactccat tcaatcattc
     4081 cccgtacctg gttttcggtc cgcttgtcga tctcgatcat tttccgaatg ttatctgccc
     4141 aactttgcag attcggcttt ttgatttcct gattctgaca aatctgtttg aataactcct
     4201 ccgcgagaat gtagtaaact gagtcggtgt cgtaaacacg ctttttgcgt ggttgcgacg
     4261 atgatgtttt attattcttt tcattcttat aattcttttc attcttgttt gtgtgcactt
     4321 gttgttcact tgttgtgcgt ttgatgttca tttgttgttc actgctttgg tacaaagacc
     4381 agttttttat tgatataacg ctgtatttcg tagttgattt gatgttcaac attccgtttt
     4441 tttcaaattg ttttagccat ctccatacag aaccgctgtt cacttgatgt tcacgtttca
     4501 cgccttcgtt catctcagat gtgatagcat cgcgccctgt gacgaattct ccgctgttca
     4561 cttgtatctc ttttccatta aaaagtatct tgcgattctc atgactcgct ttcatcaaac
     4621 acaagctcca taatttatac atataaggat tggtccaaac gaatgaattc atcacttttc
     4681 gatgtaattt gacatatcct gtgttcattc gtatttctcc tctagaaaag caagggagaa
     4741 agctccctca ttatttgttt aacggcggat tagatgcatc aaataatcca gtttgtacat
     4801 cttcattttc ttcagatata acctctgctt cttttctttc aggaatatct tcttcaactt
     4861 ctgtttcagc aataatgctg ccgtcttctt gaaccctttg gactctctca tccgatgtgg
     4921 tggcttcttg catttcgatg gacaagattc cccatttaga aagaagattt ctcaaaacag
     4981 tttttcgtgc cattgcattg taatcagatg cccacacacc acttaacttt gtcttatcgc
     5041 gatctttatt gttagcaatc cgatgagctt cgatttcttg tttggtccaa tagacagttt
     5101 ttttgaatcc attcagtaac tcgaaatagc caacatatcc aatgacttca tcagacgttc
     5161 taccatttgg atcaaactca aactcttctg tcagtcggtt ccagcttttt agttctcctt
     5221 cgtaaacttc gatcacattt aatgctttgt atttacctga tcgttgggct aattggatat
     5281 atcctttata gccaagcatg aattgagctt tcttttccca ttttcctgtt tgcttgtttt
     5341 tactattgaa tggaactaaa tatgcataac ctaaattctt atctagccca agatttaatg
     5401 ttgcagcagt taacgcacca ctcatgatag acatcggttc actatctgca agataactgt
     5461 cattagatac aagagtcata acattcgaca taaaagcatt agcattgtca tgaagtactt
     5521 cttcaaattt ctttctcatt gttggtgtat tcattagagc tttaagccct aactgtcctg
     5581 gtgcaacttg tttctgtggc ttttctgcca attgattttt taacgattca tttgttgcca
     5641 tattatttga tccccttttc ggttagcctt cttgattcag taacgttata aatctcttca
     5701 tcatttgcga catctggata tttctctgct agtttcttcg agttcatacg tctcgtacgg
     5761 acaaatttcc aactgatgat gtttttttga gtgataccga tactggcttc acgtttacct
     5821 agctcgctga taatctcgtt gtctacttga cggatagctg attcaatttc ttttttcgtc
     5881 cgtttgagtt ctcttttttg ctcgataagt tcatcaaaac gtgatggtag agctgtttga
     5941 ttttcttcta catctgcata tttttctttt aagaagtcag cagtcgctcc acttccgtca
     6001 attacaggct cgatacctcc aagaacgttc gtttcccaaa actctactaa ttgttcagtg
     6061 attgtatcga tcagctcttg atctcgttca atccgcttcc aaatgaattt ttgtccaccg
     6121 atcaaaacag cgatataaca atagtctttg tttaaaacgt tcatgtaatg ctgaacttga
     6181 cagagatagc taagcggtac ctcttcacct tcccactctt ttccgagaaa ttgattggct
     6241 gttttacatt ccagaatggc attttctccc actacttccc gatcaatatt tgctcttaaa
     6301 aatggatgta atggatgttc aaatacttgg tttcttctgc gtactttttt gcctgtacgt
     6361 tcctgaaact ctttagcaac cacttcttct aaaacattac cccaataagc tggttcgctt
     6421 tctaaatctt tcaattcaat ttgacccgtt ttctcaagcc acaattgata aggagatttg
     6481 tatttgttta atccaagtac ggttgcaaca tctgagccac caatacctct tttgcggtct
     6541 tcgagccatt cttgatggct catttctaag gtagatttac tcatcttcat cctcctcatc
     6601 aattggcgct tcatatggtg gtttagcata atcaggatca gttaaatagt tgtcaagatt
     6661 tgctaactcg ttcatgttta acctcttttc tgcttgctaa cacgcaaagc acaagcatgg
     6721 taattgcgaa taacaagcca aagattacat aacttttcgc tacaatcact aacgtgaaaa
     6781 ataaaataat gagtagatca attgtttttt cattcatcat tgaaattccc ccttgagaat
     6841 acgagtcatc acatcagcta aatcttcctt gttattaata acaaaagtat gatttgaagg
     6901 ttgattttct tcttcatttt tcgcttgttc gtaagcatcc acagcaattc tacgcataat
     6961 atgtgctgac aatgggagat tttcatctaa atgttcttct tgcatagcaa gacaaagtaa
     7021 aatttctgtt gtctcaccat aaacgagact tttaagttcc ccttcttttt gggctgtaca
     7081 aataatagaa acaccttgct tttggcattc acgtttcaat tcttcgataa gattttcaat
     7141 tttcttattc atgtgataaa atctccttag gtataatttt tggtttgtga ctcattgctt
     7201 tggacggctg agtcactttt ttatttctaa ccacttcttc tggcgttcac gccattgttc
     7261 ccctagcaat ctaggtttca tttcctcgtg gtattcctga tttagttttc gcatcaagtg
     7321 ttgatgctct tgtaatgtca tttgatccct cctttcgttt attgaatttc tccatttcta
     7381 tttgataaaa tatttctata gaaatggagg tgaaaactat gggaaaaaat caacatgttg
     7441 tacctaattc caatggtggt tggaatgtca aaggtgctgg aaattctaaa gcgactgcac
     7501 atactaatac aaaatcagaa gcagtaaaaa tcgctagaga gatttctaaa aatcaaggat
     7561 ctgagctatt tattcatggc agagatggaa aaattcaaag ccgtgatagt cccggaaacg
     7621 atccacaccc accaaaaggt taatcatatt taggagttaa tcgaatacga taaccgtcag
     7681 caggagttgc atcggtcaat gtaacttctg ctactttttc cccttcttct gtttcaataa
     7741 ttaatcttgt gtaattgttc tcattcaaaa tccctatttt tgattcttcc ttcatgtcat
     7801 ccctccttcg tctctcgccg tcaccacctc caagaagagg agttgccaaa atgaagaaac
     7861 gtggactttg gcaaacttga aaggagagca gcaactcctc ttttcggaag tggcgagtgt
     7921 gtgatataat attattgttc tatggtgccc actacttgct tgccggctgt gtgggctttt
     7981 ctttttgttc aattatggtt gccaccacag taactcccca atgtctagaa cgtattctag
     8041 ccatttcatc tgctaatctt tgaagattag gtgtgccatt caaagtgatt tttggcttgt
     8101 tactcacaat ctcacctcca tcagtttgtt ttcttttcac ctcttaatag aattaacttg
     8161 aaagcgaggt gaattgttat gggtaaaaat caatgggtat ctcctagaaa tggcaaatgg
     8221 gctgtacgtg gtgaaggtaa ttccgaagat acaaaactat ttgataataa atcagatgct
     8281 gttgagtatg gtaaaaagat ttctaaaaaa cagcaatcag agtttattgt ccaaaaaaag
     8341 aatggacaaa tacaatcaaa agatagtttt ggaaacgatc ctatacctcc acgtgataaa
     8401 gaacattaat catatctagg ggtcattcga actcgaaaac cttcgcatga ttcaatatca
     8461 ttcgccgtaa taactgcgat tgttttcggg ttctgctcgt ctgtttcgac aacaattttt
     8521 gtatattctt caagagtcgg tacttttata ttcttctctt ccactattca cacctcctag
     8581 ctgacttgtt ttgtttcacc tgttattgat ttcggttttt ccgaatcagg gtctaaaaaa
     8641 atatcggaaa ctctaacacc caatgcctta gcaatttttt ctagagtggc gtactttccg
     8701 ttcctcattt tatctacatc gttttcgtaa ataaatattg ttcttgatgt cactttgctt
     8761 ttccttgaca gttcttcttt tgaataaccc tttaaagctc ttaattgtct caatgtatat
     8821 ttttctttct tcgcggtatc catattttat tcgcctcctc tctttaactt tctgactaaa
     8881 ttatatactt cggtttttcc gaagtcaagt gcatatttcg gttttcctga aatcgatttc
     8941 tattttgcgt aaatacttct ttaaaaacac taaaatattt tctaagaaac ctgttacatt
     9001 ttttccgaaa cggtgttaca attaactcaa ggaggtgtta ctatgttcgc tgaaaatcta
     9061 aagtttctta gagaaaagta taatattgat caacaagaac tagccgaaaa actaggaaga
     9121 aaaagttcat cctcaataag cgaatgggaa aaaggaaaat atacaccaaa attaaaaacc
     9181 ttaaatgaga tagctaagat attcaatgtt gatatagatg atttgatgaa tattgattta
     9241 tctatatcta atcagatata taatatcctt cctatatata atcagcttaa cgacgaaaat
     9301 aaagttgcta cctatgaatt tgcaagtaat cgattagaag aacaaaaaca agaaaatgtc
     9361 ctagattttc ctaaaaaaga aaaaatccca actgttcaca attcggcttc ggctgccaat
     9421 ccaacagaac ttacttacgg tgatacggtt gtcgaagagg aagaatttga gagagttcca
     9481 agtgctgctg acttcgcagt acctattata ggcgattcaa tggaacctgt tatcaggaat
     9541 ggacaattcg tattcgttaa agaacaaccg gacgtagaag atggggaaat tgctattgtg
     9601 gaattaggtg gtgacggagt tacatgtaaa gagatatata aagattacga aaatcaaaca
     9661 atcattttac gttctataaa cgacttatac gaagatagaa ttgtatcgcc agaacagatt
     9721 agaattatcg gaaaagttgt attttaaaaa aattcccatg ccggagccgt caacttcggt
     9781 aagggtttat atgattttga caattatttt ataaaagaag cgaggcgttt tgaatgggag
     9841 tactattgtt tttagttgga atagttttaa taattatggg tagtagcaaa ttatctgaag
     9901 ctaaaaaatt aaaagataaa acttctgcct tagagagtaa ggtaaaggaa tattctcaag
     9961 aattaaatgc tagggaaacg tttatatctg aaaaagagaa aaacataagt aaattgaata
    10021 aaaacttgtc aacgctagaa aacaaaattg atattcttaa tgctgaaagc cagcggttag
    10081 acgatgaaat taatcaaaaa tatgacacat ctctggtcac tatctataaa tcagtaatta
    10141 ttccgaatat agatgaaact tcttcatccg aaataaaaaa caaactttct ttattaaaga
    10201 ttaaagaaaa ggattttatt ttagacggtg caattaatat tccttcttat ttgaataata
    10261 gagaagctaa taatattaaa aagaaaatgc ttataccatt agaatcagaa ataactggac
    10321 ttctcaataa acttactatt tctaacgtag attcaactag agaaaaaatt ataagaactt
    10381 ttgataaaat aaacaagctt tttaaaaacg atgaagcaga attcaaaaaa gaattacttg
    10441 aattaaaact tcaagaatta gaactcaact attcatacat agttaaagtt aacgatgaaa
    10501 aagaacagca aaaagcaata aaagaacaaa tgattgaaga agaaaaggta agacgtgaaa
    10561 tagaacgtga aaagaaacgc attgataaag aagaacgaca gtttaactca gagataacta
    10621 agttaatggc atacatgcaa aaagctagtg ctgatgttga aaaagaactc tatgctgata
    10681 aaattaaaga actggaagag aaattaagag aactagaaac tgtcaaagaa gatgtattgc
    10741 aaagagaact taatactaga gctggctatg tttatgtcat cagtaatatc ggatctttcg
    10801 gtgaagatgt ctataaaata ggaatgacta gacggttaga acctatggat agaattaagg
    10861 aacttagtag cgcttctgta ccttttgaat ttgatgttca tgcaatgata ttttcagaag
    10921 atgcaccaac attagaaaat caactacatt cgcattttag aaaaaacgaa gtcaataaag
    10981 ttaatcaacg aaaagaattc tttaaagtat ctcttgatga aattgaaaaa gttgttttag
    11041 aaaattataa cggaactgta tcttttacta aactagctca agctgaacaa tacagaagat
    11101 cattagaatt aacagatgtg tagtaaaaag attagccttc gggcttttct tttacaacga
    11161 taagaacata cattcgaaag gagataacat gaaacgagta gcattatata tgagagtatc
    11221 aactgaacaa caagccaaac atggagatag tcttagagaa caaaaagaaa ccctctatga
    11281 atatatcgaa caacacaagg atttgaaagt agttaatgaa tatgtagacg gtggtatttc
    11341 tggtcaaaaa ataaatcgtg atgaatttca aaaactatta caagatgtaa aagaaaataa
    11401 aatagatcta attttattta caaaattaga tagatggttt agaaatctac gacattattt
    11461 aaacactcaa gaaattttag aaaaacacaa tgtttcttgg aatgctgttt ctcaacaata
    11521 ttatgataca actacagcgt atggcagaac tttcattgca caagtaatga gttttgctga
    11581 attggaagcc caaattgatt cagaacgcat caaagctgtt atggcaaata aaattgcaca
    11641 aggtgaagta gtaagcggga aaactccgtt aggctactct atagaaaata agaaattagt
    11701 aataaatgat gatgctccta tcgtgataga tatattcaac tactttcttt ctagtggtag
    11761 tttaagaaaa actgtttatt atttaggctc tcagtatgga atcgttagag attatcaaag
    11821 tgttaaaaat atgttgacga acaaaaaata cattggtgaa ttaagaaata ataaaaatta
    11881 ctgtcctcct attattgata aaaaactttt ttatgctgtt caaaaagcct tacctaaaaa
    11941 tttaaaaaca aatgcaaaac gtgattacat ttttaaaggt ttattaaaat gttctgactg
    12001 tcagggatca gtagctggtc aaactattaa agcaagatat aagaaaaaag acggtacaga
    12061 atctatatac gaaaggactt gttatagatg tgtaaaacga agaaataata aattacgttg
    12121 cacaaataag cgcgcttttt acgaaaaaaa tttagaacgt tatatttttg aagctactaa
    12181 acaaaaattt gaacaaatac aaataaacta ttcaaaaaaa caaccaaaga ttattaaaaa
    12241 gaaaaatagt aagaaaaata ttgagaataa attagataga ttgaagaaag cctacttgaa
    12301 tgaagttata gacttagagg aatacaaaaa agatcgagaa gctttaatga aagaattaaa
    12361 tgaaattgaa gtagagccag ctaaaataga tataaaaaat gtcgaattta ttctatcaaa
    12421 agaattcgat gaaatttata aagaatcttc ggaagaagaa aaaaatgcat tatggcgttc
    12481 aattatcgat aacataatag tatttccaga tggcaatata acagta
//
LOCUS       DACEZN010000077        12468 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEZN010000077 DACEZN010000000
VERSION     DACEZN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.077"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(151..900)
                     /locus_tag="IXH60_001676"
     CDS             complement(151..900)
                     /locus_tag="IXH60_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1990597.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            1229..1609
                     /locus_tag="IXH60_001677"
     CDS             1229..1609
                     /locus_tag="IXH60_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1990598.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            1705..2214
                     /locus_tag="IXH60_001678"
     CDS             1705..2214
                     /locus_tag="IXH60_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990599.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            2291..2470
                     /locus_tag="IXH60_001679"
     CDS             2291..2470
                     /locus_tag="IXH60_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990600.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            2525..3088
                     /locus_tag="IXH60_001680"
     CDS             2525..3088
                     /locus_tag="IXH60_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1990601.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(3135..3650)
                     /locus_tag="IXH60_001681"
     CDS             complement(3135..3650)
                     /locus_tag="IXH60_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990602.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(3676..4545)
                     /locus_tag="IXH60_001682"
     CDS             complement(3676..4545)
                     /locus_tag="IXH60_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1990603.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4671..5351
                     /locus_tag="IXH60_001683"
     CDS             4671..5351
                     /locus_tag="IXH60_001683"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1990604.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            5504..6484
                     /gene="pta"
                     /locus_tag="IXH60_001684"
     CDS             5504..6484
                     /gene="pta"
                     /locus_tag="IXH60_001684"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1990605.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            6607..7080
                     /gene="tsaE"
                     /locus_tag="IXH60_001685"
     CDS             6607..7080
                     /gene="tsaE"
                     /locus_tag="IXH60_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1990606.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            7073..7597
                     /locus_tag="IXH60_001686"
     CDS             7073..7597
                     /locus_tag="IXH60_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1990607.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(8087..8620)
                     /locus_tag="IXH60_001687"
     CDS             complement(8087..8620)
                     /locus_tag="IXH60_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1990608.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(9484..10236)
                     /gene="xth"
                     /locus_tag="IXH60_001688"
     CDS             complement(9484..10236)
                     /gene="xth"
                     /locus_tag="IXH60_001688"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1990609.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            10349..11269
                     /locus_tag="IXH60_001689"
     CDS             10349..11269
                     /locus_tag="IXH60_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1990610.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            11342..12277
                     /gene="murB"
                     /locus_tag="IXH60_001690"
     CDS             11342..12277
                     /gene="murB"
                     /locus_tag="IXH60_001690"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1990611.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
BASE COUNT         4133 a         2211 c         2517 g         3607 t
ORIGIN      
        1 aagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt tcagcttatt
       61 tccgaaggag ttgcttctga ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg
      121 ttctttactt ttgtcccacg ctcagttttt ttatgcgtgt tgttgtgcgt tgtagtattc
      181 ttcttccatt ttttgcatac gttcacgttc tttttggtta taccaaggtg aaacggtaaa
      241 tgcaaggaca tcattcagca agtaaacaga gctgttcacg aacatcgcca aacttgcaga
      301 tccatgcgta aatgaaatat accaaaggac catttgagca agccctgaag ctaaccacca
      361 gtaatattgg ttgttgtaac gcaagaaact gatgatacca gcagtcaagc taatagaaaa
      421 agcaatggca tctacccacg gccgaggatc atctgtataa cgaccaatca ggaatcctga
      481 aacaagatag acaaggaaag tactgatgat tgcgataaac cagctttttc cagtgaattt
      541 tcggattttt gaagccatat tatgattcca ttctttactt aacaatacgg ggatatccaa
      601 tgtgatcata taagcaatct gttcaccgat acttaaatag ttctttgctg aaaacccaac
      661 gataataaaa caaatcgcag acaaaatacc taaaataccg ttgactgatt ttgccgcatt
      721 gatggataaa atacacaata caccaagcgt ggtaccgata aatgtaataa cggacagcca
      781 agtgatggga ctattgacaa gagtcatgac ctggcagcca agactgaaaa agaaaagata
      841 ataattttgt tgcggccagc cttttagctg atgagctaaa aactgaaaat atgacttcat
      901 gtatgaggac tccttaagac actaaatgta gtgtgttctg tatttactaa cacaatctat
      961 tgtatgcttt tcttgaaaaa aaactagtct ttcaattata aataatttgt gttatgataa
     1021 ttttctgttt gttttcttga aaatataatt tcttactttc atttattata ttgggaacag
     1081 gaagagtgtc ttggaggacc tactttctgt ttttttgcct tttttcttat aaaaaggaaa
     1141 aacagctatt tttttcaatc tgacacattt acaatggaac tggagattaa tatagatccg
     1201 agcttaaacg aagaatagga gtgctcatat gaaccttaca acgatccatc atatagccat
     1261 cattgtttca gattatcaga aatcacgtga tttttatgtt aataaattag gatttgaagt
     1321 aatcagagaa aattaccgca aagaacgaga tgactataaa ttagatctga aactaggaag
     1381 tgctgaattg gagatttttg gaattgctaa tgcgccacaa cgaccaaact atccagaagc
     1441 ttgtggcttg cgtcatcttg ctttcaaagt agacgatatt gaagaaacaa ttgcagaatt
     1501 gcagtcctta gggatcgaat cagaaccgat tagagaagat acctttacag gaaaaaaaat
     1561 gacgtttttc tttgatccag acggattgcc attagaactg cacgaataaa gataacaaaa
     1621 ttgtcacttt tttttcgttt cattcgattt ttcattcgtt catttcttga tattctttaa
     1681 aaaaaagaaa caggagaatg aagaatgaaa aaaatgatgt ttggtgttgc tgccctcatc
     1741 gtaataggga ttaccggtat gacgggactg ctcaatgccg cttttcacat ggatgatatt
     1801 agtgaccagg aatatcggat ctcattgaaa aaagcagcag caatcgtaaa aaacgaaaca
     1861 aaagaacata acatatctgc tattcatttt gcagttgaaa aagatttgga gaatcctacg
     1921 aatgcaacat atgaatacca gtttttttgt gaaggacaag tcatagcagt caatccatcc
     1981 actgggaaaa caaccgtacg aaatgacacg gaaaaggccg cagaacatct ttttgatata
     2041 gaaaaaacgg aagagttcaa acaaccgcaa gcagcgatga aggaagcagt cagctactac
     2101 gggaaaaaag caaaagtgaa agagtggaca cttctagtca aagatgaacg tccttattat
     2161 aaagtccgat taatagatgg agaacaggac cgtcaggtat ggatatctgc atagaagaag
     2221 gatattgctt ctctttttta caatataaga gtaagctaag agagtaaaga tgaaaggaag
     2281 ttgagataac atggaaaaaa tgaaatgccc taattgcgga aaaaaatttg catacgaaga
     2341 agtcaataat gtagtcgaac ataatgataa agaaatgcca gtcgtctgcc cttattgccg
     2401 aactgaagcg gcaagaatcg ttactcatgg ttatttcatt actgaaaaga ttgaagattt
     2461 tttaaaataa cgattttgcg atttcttttt ctgcatgtta gaatggagaa aaggagagga
     2521 aactatgatt cgttttgcga gaaaagaaga tgctccagaa atcgccccat tgatcttagt
     2581 gattctaaaa gatatggaac tgccattttt gaagcagaca ggggaacaaa agactttaga
     2641 gattttagaa gaagcaatca ctgatccaaa ttatcgttac agttattcac gaggtatcgt
     2701 agaagaaata gacggtaaag ttgcaggagt tgcttacggt tatacagatg aagaagaatc
     2761 aatcatcgat cagccactgg tacctatctt agagaaatat cagctagatg cgtctacacg
     2821 tttgttcatt gataaagaaa cgtttcccaa cgaatggtat ttggactcta tcagtgtatc
     2881 agaagatttt agaggacaag gcatcggttc gcgattatta gaagcattac ctaaattggc
     2941 gaagaaagct aatcgctccg tcatcggtct aagtgtggat gaaaaaaatc caaaggctaa
     3001 aaaattatac gaacgccatg acttcaaagt tgtcggtcag cggatgatca gtggtcatct
     3061 ttatgatcat atgcaaaaga atatctaaaa agatgtacct gaaatgcgta tttccgcatt
     3121 tcaggtacat ctttttaact taagttaagt cggtaagaaa caggcttatc gcgacttttg
     3181 tctttacggt agagccagta atctaaaacc gcagcaatac aaatacaaac tggagcatct
     3241 tcatcatttg agacatccaa tacatagtaa tctcctgaaa aaagtgttgc tttatccatt
     3301 ttcatgattt gctgattgaa atgatggatt tgatattgat gattatagat atcaccttgg
     3361 actgtccagt gaagtctacg aatataataa aagtcaccgg gccaattgaa tattttctgc
     3421 atggttccca ccttttcgaa ctctttatac agctcaaaac gcgtaccgaa tgtcaggctt
     3481 atctgcttga tgtgagcaac gagatcgcca ttcatggcat aaagagacaa tgcatcgccc
     3541 tttgttcccc agcgaccaac catcaaaaac agggaatgtc ctttttcgtt tttgacaatt
     3601 gtccgagttg cacgtccaag ctgcttttcc tgaatgaaaa attctgacat ctatatccct
     3661 cttttctaga taaaactaca agtcttctgc aatcgcgcgc aatatggctt ttcctacgcc
     3721 agattcaagg tttgtgtccg ttgtatattt ggcataatct tttacttcga tttctgcatt
     3781 tcccattgcg aaactgactc ctgctacttg cagcatgctg acatcattga agttatcgcc
     3841 aatcgtcatg acttcttcta aagaaatccc acgctcgtga gcgacatggg caacagcaat
     3901 tcctttttga gcgtttttat ggttcacttc gatattgttt tgaccagagg aagtaacggc
     3961 tagatcgcct aaaagagaaa tctgctttcc aactggacct aatacagctg gcccttctgt
     4021 atgaaaacaa ataattttta aaacttccag attcttttct tccaaaatag cacgaatact
     4081 gtccacgtat gtgatgtgca ataactccag cttagctgag gccatggcaa tcgccatttt
     4141 atacgtaagg tgaggtaaat gggtagccac catggaagca aaactttcaa ttcgtttcgc
     4201 ttgattttca gaataaagac catcgttggt tgctacttca tagtaaatac cgttagcatc
     4261 tagaatatct aaaacagaga ttgcttgatt atttggaatc gacacggtaa acaatgaatt
     4321 tccttcttta tcaaatacct gtgccccgtt caaagtgatc atcgcacaat cgattcctac
     4381 ctcatctaaa gctgcgcgag cttcttgata gtttcgacca gtagcgacca tgaactcaac
     4441 accggcatca tttgcaaaac gtatcgcctc ggcattttca ggagaaacac tcatatgggc
     4501 atctaataat gtgccgtcca tatctgatgc aattaatttg atcataatga ttatcgttcc
     4561 tttctgcttc ttgttcctag tttaccatat taatcaagtg ctgagggtga agattgtccc
     4621 ttgtgattga agaaaatgtg agttattctt tttattagga ggtggagttt ttgaaagaaa
     4681 tcattcataa cagttggcaa gaagttttgt cctcagaatt ttcaaaagat tattatctgc
     4741 atctaagaga atttttaaaa aaagaatatg cgtcccaaaa gatccatcca gatatgtatc
     4801 atatctatga agcattggaa ttgactcctt atgaagaagt taaagtggtg attttaggac
     4861 aagatcctta tcatggagaa aatcaagctc atggcttatc tttttctgtg caacctggcg
     4921 taaagatccc gccatcgtta agaaatatct ataaagagct ttatgatgat cttggaatcg
     4981 cacctgttca acatggaaat ctagtgtcat gggctaaaca gggagtcctt ttactcaaca
     5041 ccgttcttac cgttcgggaa gggcaagcgt attctcatcg cgggaaaggt tgggaaagat
     5101 taacagacac gatcattgaa aaattaaatg aacgggaaaa accaatcgtg tttatcttgt
     5161 ggggaaaacc ggcacaagaa aaaatcaaaa tgatcgataa gagtcgccat atcatcataa
     5221 catctccaca tcccagtccg ttatctgcaa gtcgtggctt ttttggttcc aaacccttct
     5281 cgaaaacgaa tgatgcgctc cttgcgttag gagaagaacc gattgattgg caactgcctg
     5341 aaacagtgta ataaaacagc gctttcctgt tatagtgcag ttcgctttgt atgtgaaaaa
     5401 cttaattttt ccagcatttt tgactgatac agataaaata aactgttctt tttttctgga
     5461 atagtgtatg ataatacata taaaaacgtc tggaggtcat cttgtggaat tgtttgatag
     5521 tttaagattt aaaattgttc gccgtgggat caaaatcgtt ttcccggaag ctactgatgc
     5581 tcgtatttta ggcgcagctg cccggttgaa agcagaagaa cttgtggaac ccattttagt
     5641 agggaacgaa gaagaaataa gaaatgcagc gcatgccaga ggaatcaaaa catctagctt
     5701 tacgatcatc agccctgata attacgataa gtgggatgaa atggtggata cttttgttga
     5761 acgtagaaat ggaaaggcat ccaaagaaca agcagaaaag attttaaaag atgtgaatta
     5821 ctttggtacg atgcttacgt atatggggat tgcagacgga atggtctctg gagcgatcca
     5881 ttcgactggc gacactgttc gcccagcact tcagatcatc aaaacaaagc ctggcatcag
     5941 tcggacaagc ggagcgatga tcatggtacg tggaagagat caagaaaaat atgtcttttc
     6001 ggattgtgcg atcaacgtga atccaactgc gcaagaattg gctgaaatcg ccgtagattc
     6061 tgcaaaaaca gcagaattgt tcgatatcga accaaaagta gcaatgatga gcttctcgac
     6121 aaaaggctca gcgaaatcgc cagaagtaga caaagtagtt gaagcaacta aaatcgctaa
     6181 aaaactagcg ccacaataca tgatcgacgg cgaattgcaa tttgatgctt cttatgttcc
     6241 ttcagttgca caactgaaag cgccagattc agaagtagct ggtcaagcaa cagtctttgt
     6301 atttccagaa ttgcagtcag gaaatatcgg ctataaaatt gctcagcgtt tcggtaactt
     6361 tgaagcaatc ggaccaattc tacaaggctt gaacaaaccg gtttctgatt tgtctcgtgg
     6421 agcaaatgaa gaagatgtat ataaattatc gattatcaca gcagcacaaa cattgattga
     6481 ttaacaaata cacgcgaact agccaaaaac agcagctgct gttcttgact agttcatttt
     6541 ttctttaaaa aaacttcatt acgcgatata ctggcaaaaa gaaagaagag cgggagaaga
     6601 tgaaaaatga tcgaactatc aggattaaca atgacagaac aattcgcaga ggcaattggt
     6661 cattccgcaa tgccaggtga caatctgata ttgactggtg atctaggtgc gggtaaaaca
     6721 acgttgacga aaggaattgc ccaaggatta gggataacgc aaatgatcaa aagtccgaca
     6781 tacacgatta ttagagaata tagtcaaggt cggattccgt tgtatcatat ggatatttat
     6841 cgggtagcag ctagcggagc agatttaggg ttagatgaat attttgaagg tgatgggctt
     6901 tccgttgttg aatggggaaa tctattagaa gaagcactgc cggaggatta tttggaactg
     6961 attttagaaa aaagtgatac agatttagaa aagcggtatg tgaaactaat ctcatacggg
     7021 gagcaaagtg aaaccttcaa acagcgaatt ctagaaaaat gggctgaaaa tcatgagtga
     7081 agaagttggg atcatcttgc gggaagcagt gccaggagat gcaaaagaca ttcttttgat
     7141 gatgggacaa gttaataagg agacagaatt tttagttttg gatgaagcag aactcctgtt
     7201 accaccagaa accttagaag aagagttgga ttatatatac gaaagcaata ataatttatt
     7261 gctgcttgca atctatgaag ggacgattat tggaacagca tctgtcaagg cagacagtca
     7321 atttcgtttg tctcacgtgg gagaagtcgg tatcagtatt ctgcaggaat attggggaat
     7381 gggactaggc actcttatgc tagaagaaat catcagttgg gcgaaggaaa tgggtgtact
     7441 ttttcgattg gagcttgacg ttcaagttcg taatgaaaga gccgtacacc tttaccgaaa
     7501 aatgggcttc caaatcgaag cggtcatgcc tagaggggca agaacagatc taggtgaatt
     7561 tttagatgta tataaaatga gttatttgat tgaatagaag aaataggaaa agagtgagaa
     7621 actgtcatcc ttttgagaag gcagaaaaac acacgaaggg actgtgacaa aagtcgtgtc
     7681 gcagtccatt ttttttgaat aaacggtgtt caatcagctg accttcggta ttagctcaaa
     7741 aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaaacg
     7801 ctgatttcac aacctcttgg taaacggtgt ccaaaagctt gcatctgcgg taataagtcc
     7861 aaccaaatga aaaatatgag aagctatttt tgtttggctg ttcttattac ttgatgcaga
     7921 acagcttttt cacaacctct ccaataaacg gtgttctcct gccagctcct cggtcaccag
     7981 tcacaatatt taataaacat gagaagctat tttttgaaat tgctccttgt gactcggagt
     8041 gaaacggcag gctcacgacc tctttgtgtg tttcataagt attcttttac tttatagtca
     8101 caagtttttt caatggatct gtcccgaatt ttttttcttg ataaagcagg atttccgcac
     8161 atgcacgact atcttccaat gcatcatggt gatgatctaa ttggatgttc aaattttcgc
     8221 acacagtgtt caatcgatga tttggaaatt caggaaacag gcgacggcta cttttcactg
     8281 tacataaaga aagataatta ggctgttcaa gatgataata atctaagcat cccgctaaga
     8341 cctttgtatc aaaagcagca ttatgtgcaa cgactaaacg atttttttga aaatatggtt
     8401 ggatagtttg ccagacttct gggaatttcg gcgcattgcg tacgtcttct tgatggattc
     8461 cgtgaatttg gacatttcgc cagaaaaaag gagtctcagg ctgtatcaat gtatagaatt
     8521 catcaacgat ctggctattt tttaccatga ctagcgcaag agaacaagca ctataaggct
     8581 gatgattggc agtttcaaaa tccatggcaa caaaattcat ttctgtaaaa cctccttggg
     8641 aaattactgt aacatacttt tgaaaaaagc aaataaattt aacaaaaaac atagaatttt
     8701 ttatgtaata tttgttgaga aaagctagtt tttgaactca ttccttgtga tttggagtta
     8761 gatggggatc atgacttctc ggtattaagt caaatttatg caaagttgtg aggaaccatt
     8821 ttcaaaaaaa tttcttacga ctcgaaagta ggcgactttt tcacaacttc tccactaaac
     8881 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat agcaaaatat gaggaactat
     8941 tttgccattt gtttctctaa tactcaggtc aggacgctga tttcacaact tctcaataaa
     9001 cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aaacgaaaaa tatgaggaac
     9061 tatttttgtt tggctgttct tattacttga tgcagtgcag ctttttcaca acctcttaat
     9121 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgagg
     9181 aactattttt gtttggctgt tcttattact tgatgcagtg cagctttttc acaacctctt
     9241 aataaacggt gttcaaaagc ttgtatctgc ggtaataagc ccaaccaaac gaaaaatatg
     9301 aggaactatt tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     9361 ctcgataaac agtgttcaaa agccagtttc tcggtattat gccaaattta tgcaaaatgt
     9421 ggaaagccat tttcacaaaa tttttcttaa tactcgaaac taaacgactt tttcacaacc
     9481 tctttatttc aaatctaatt ctactggaca atgatcactg cccataatgt ccgtatggat
     9541 ctttgcatca gctagtcctg gctctaaatc tttgctcgtc aaaaagtaat cgatccgcca
     9601 accagcattg tttttccttg aattgaatcg gtagttccac caagagtaga caccttcctg
     9661 ggttggataa aaataacgga atgtatcgat aaacccgtta tccaacagtc gagaaagagc
     9721 cgctctttct tctggagtga atccagcatt tttttgattc gttttccagt ttttcaaatc
     9781 aatcttttga tgagcgacat ttagatcccc acacacgatg actggttttt gtttttttag
     9841 attttccaaa taattataaa aggcttcttc ccattctaat cggtaatcta aacgttttaa
     9901 ttcgctttga gaatttggcg tgtaacaggt tacaagaaag aaatcggaat attccaaagt
     9961 aatcagacgg ccttctgtat catggatatc gatacccatt ccgtaagtgg cattcaatgc
    10021 tggtttttta gcaaaaatcg ctgtgccgga atagcctttt cgttccgcgt aattccaata
    10081 ttgataataa cctggaagat ctaaatcaat ttgtcctgct tggagtttcg tttcctgcag
    10141 acagaaaaaa tcggcgtcta actcatgaaa aacttctaaa aaatttttat ttacgatggc
    10201 acgaagcccg tttacgttcc aagagataaa tttcaataaa agcacactcc ttcgaattaa
    10261 tttccttacc tttgcgatac tattgtacct cattttagta aaaaatggtg taagatgaaa
    10321 gtaactaaat gatccaggag gaaagtagat gaagattggt gtaatcggtg tagggaatat
    10381 cgcagaaaaa gcatatttac caacttatgc gaaaaaacaa ggaacacttg atttttattt
    10441 tgcaacaaga aataaagaaa caaaagaaag aattcaaaaa gagtacgggt tccaacatct
    10501 atatgaaacg atcgatgagc ttttagaaga aaaaatcgaa gcatgtatga tccacgcagc
    10561 aacaaaagta cattatgaat tagccaaaaa atgtctagaa aatggtatcc atgtttatat
    10621 tgacaaacca ctgagtgtcc atctaaatga agttcgtgaa ttgcaagaat tagctgataa
    10681 gaaccaaata attttgatga tcggtttcaa ccggcgcttt gctcctatgg tagaagaatt
    10741 gaaacgtatc ccagataagc ggttgatcca cctgcaaaaa aatcgaatag cggcaaaaga
    10801 aacgacagag ttcgtccttt atgatttgtt tttacatctt attgatacag ctgtgtattt
    10861 attagatgaa cccattttga gaacaaaata ccagataaga gaaacaaagg aatttttgga
    10921 atatgccatt ttacaattag aaacacaaaa tcaaacggct attttaacga tggatttagc
    10981 aagtggcgct aatatagaaa aatatcaagt aacaagtaaa aatggtactt atgaagtaaa
    11041 cgatctgacc cagcttgtca ttcaagatgc tgatggaaaa aaagtgaaag aatttggcga
    11101 ctggacaaac acgctagtca aacgcgggtt tgagccaatg gtagaggcct tcttttctca
    11161 gatccgttta ggtaaatcgg aaacagaatt gcttaaacaa aaagaagttg taagaagtca
    11221 tgaattatgt gaggaagtgc ttaaagatca ctaccgtcat caactttgaa caagactggc
    11281 aagattgtat taagcgtgat agaataatga ggacgtttga gaaaagaaag gaaaatcctc
    11341 agtgaataaa gaagaaatag ttgaaagctt agccacgatt actatattgt ttgatgaacc
    11401 attgatgaac tatacgttta cgaaaacagg tggaccagct gatgtccttg cattcccgaa
    11461 aaaacaagaa gaagtaaagc aaatcatcga ttattgccga atccatgata taccttggat
    11521 ggtattagga aatgcaagca atctgatcgt tcaagatggc gggattcgcg gtgtagtcat
    11581 catgctgact gaaatgaaac agattcatgt caaaggaacg atggtcattg cggaagcagg
    11641 tgcctcactg attgatacga cttatgcagc tttggcagaa tcattgactg gatttgaatt
    11701 tgcttgcggc atcccgggaa gtgtcggtgg agcggtttat atgaatgcag gtgcgtatgg
    11761 cggagagatc aaagatgttt ttgctgaagt ggatcttttg cttgaagatg gaacattgaa
    11821 aactttgaca aaagacgaaa tggtattttc ttatcgtcac agtaaagtcc aggaattacg
    11881 tgctatcgta ttggaagctc gattcagttt gcaaacaggg gattatgaag caatcaaagc
    11941 aagaatggat gagttgaccg aattgcgtca atcaaaacag ccattagaat acccttcatg
    12001 cggaagtgtc tttaagcgcc cagtaggaca ctatacaggt cagctgatcc aacaagcagg
    12061 attgcaaggc ctgaaatggg gcggtgcaca agtctctgaa aaacatgctg gattcatcgt
    12121 caatatcgat catgcgacag cgacagatta tattgaattg attgcgcaca tccaagaagt
    12181 aatcaaagaa aaatttgatg tgtctttaga aacagaagtg cggatcattg gagaaaaaca
    12241 agcaatatca gaaacaagca gaagtttagc caattaataa gtaatggaac ttaaaataag
    12301 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
    12361 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
    12421 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataact
//
LOCUS       DACEZN010000078        12033 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEZN010000078 DACEZN010000000
VERSION     DACEZN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.078"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..518)
                     /locus_tag="IXH60_001691"
     CDS             complement(<1..518)
                     /locus_tag="IXH60_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1990612.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGY"
     gene            complement(766..1602)
                     /locus_tag="IXH60_001692"
     CDS             complement(766..1602)
                     /locus_tag="IXH60_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1990613.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1613..2542)
                     /locus_tag="IXH60_001693"
     CDS             complement(1613..2542)
                     /locus_tag="IXH60_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1990614.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2734..3087
                     /locus_tag="IXH60_001694"
     CDS             2734..3087
                     /locus_tag="IXH60_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1990615.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3161..3817)
                     /locus_tag="IXH60_001695"
     CDS             complement(3161..3817)
                     /locus_tag="IXH60_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1990616.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4043..5977
                     /locus_tag="IXH60_001696"
     CDS             4043..5977
                     /locus_tag="IXH60_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1990617.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6063..7286)
                     /locus_tag="IXH60_001697"
     CDS             complement(6063..7286)
                     /locus_tag="IXH60_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1990618.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7280..9178)
                     /locus_tag="IXH60_001698"
     CDS             complement(7280..9178)
                     /locus_tag="IXH60_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1990619.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9172..10290)
                     /locus_tag="IXH60_001699"
     CDS             complement(9172..10290)
                     /locus_tag="IXH60_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1990620.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10373..11206)
                     /locus_tag="IXH60_001700"
     CDS             complement(10373..11206)
                     /locus_tag="IXH60_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1990621.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11199..>12033)
                     /locus_tag="IXH60_001701"
     CDS             complement(11199..>12033)
                     /locus_tag="IXH60_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1990622.1"
                     /translation="GLVTPFVIFFIVTILIPIIISFAFSFMKIGYSFEFVGFDNYLAI
                     FRDPLFIKSYTNVLIIMAGSIPITMLLAIYFAVLLNSAQLKGKGFFRTVYYIPTITST
                     VAVASVFMTFFNPTGLFNGFLNALGINGVQWLTNPFWIRISMIITMIWLSVGYNTILF
                     LAGLQGISKEIYESAEIDGASKFRQFFLLTIPMLKPIILMATVLATINGLGNFNIPNI
                     FFGTSNGPENSALVVGVNLYKTSFEMVDFGKASAIAWTMVFVSVILSAIQFKFGGDKN
                     D"
BASE COUNT         3830 a         2420 c         1977 g         3806 t
ORIGIN      
        1 ttataacctg gcatgctcat tagataaagg atttcatcgt aatggatata gtgatcgcta
       61 ttaccataac ggacagttgt ccaccttttt ttcgtagcat gttgaacagg atagttttct
      121 ttttctttca ataagtaact ttggatgctt tttttatttt tttcataagt gaattctttt
      181 atcaaataac cactaggcgt aatctttcga ttaataacag ttattgcttt attcggatga
      241 gacgttagat aaacaatttt acacttttca ttcttttctc taagcatttc gcctagttcg
      301 ataccgctat aataggcgtt caaatcgatg tctaggaaaa aaatatctga ggaatagatt
      361 tcattatttt taatgaattc aacaaattgt ataacgttca atccctgaac gatctcaaat
      421 ttccaaggac ttaataaaat tctggattct tccaaaattt ctactagcat tttttcaaaa
      481 accaaactat catccaaaat aaatatacgc gttaccataa aaagtacctc cactcgatat
      541 tttactatca aaatcatttc atacaacttt tcaacataca tgttaggaat aaattgtaat
      601 tctcttcaca atcttttcca acacctttga aaccgatatc aaatactttc aggatgctgc
      661 ttctaaaata aatatcaaac aaatgaatac atcatgcaaa aaaagccaga caaccgacgt
      721 ccttgcgtgt cagctgtctg agctatgttt atttattttg ttcttttaaa aagaacgata
      781 cacatactga tttttcggag cagagacttt ctcagcgttt gtaacaacta cagatgggat
      841 ttcacgcttg aatggttgat aatagtctga ttggatggtt ccttctattt tataccattc
      901 atcattttta accgttactc cttccggcat atgcgtcaac aaaccaaaga ctcctgaatc
      961 tgccacacaa tggataatgc cgaatcggaa caagaagata tctgtattgc tatcgttcgg
     1021 tgtttggtaa acaaatcctt catagctgat ttttttgcca atgaattcgc tagggtaatt
     1081 ataaattagc tccataactt ctaaatagtt ttcatctgtg atcttgatag tttgcttatc
     1141 cccatacttt tctttcaact cattcatttg gtctaagtag tctgatttgt tgaaataaat
     1201 actggtatcc ggctttaagt attgtgtctg catctccgga tcccctactg attctttact
     1261 tactgggaaa ttaaatcctt tggcttctac gatcgtagtg tcgagactaa ctgttggaaa
     1321 caacgtcccc acgattactg gcaaagctag cagtacataa gcgattccac gttgataagg
     1381 ttttgacaac ccatggtcat gatcgtgctc gtgtgccttt tcttctgctt ttccgttttt
     1441 gacccacaaa tacagctgaa ctaaagccaa aagagcagat aaaatcattg aaagaatagc
     1501 taagtagcga taatggacat tgatatattg atcgagtttg ccagaaactt gcaagtacat
     1561 catcaattcg aaatacccag ataaaatcaa aaaccgcagc atattttccc tcctaaataa
     1621 ataatgtgaa tccaataacg ctaagggcaa tcagtccgat cagcgataaa ataaagcgtc
     1681 ctgtaaaata tcgtttcatc atcagtagat ttttgatatc gaccattggg ccaaagacca
     1741 aaaaggcagc tacaggtgct gtgccgaaca gacttaacaa agaagatcct ataaacgcat
     1801 ctgcttctga gcaaagagac atcgtaaagg ccaataacag catgatgata atggctaaaa
     1861 tctttgttga gccgagcgtt agcataattc tagttggtaa ataagtctgc atcgcagctg
     1921 cgattaatcc gccaatgatc aaataacgac ctgtatcaaa aaattcgtcg attgaatgtg
     1981 tcaacacatg cccagtttga tggatcagct gattttttgg ttcattcttc tcactttgtt
     2041 cttcttcatg gacatgatga tggtgatttt ctgctacttt agacgtgatt ttttctttca
     2101 atacagcgtc tttttgaaaa taggcaagcc agacaccaat cacaagggca acaaaaaagc
     2161 tgcctatcac ccgccagctt gccatctgcc aagaattgcc aaatgcgata aaagtggaaa
     2221 aaatcacgat tggattgatg atcggagcag tcagcataaa agcaaacgct gtgtgaactg
     2281 gtacgccttt tttaacaaat tgatgaacga tcggtacgat gccgcattca catgaaggaa
     2341 agaaaaatcc taagatcgaa ccagtaaaaa tagagaagat acgattttca ggaagccatc
     2401 tcttcacacg ttctggggtc aaaaatactt gtaaggcgcc tgagataaga cagcctaata
     2461 atacgaaagg taatgcttca ataacgatcg ataagaaaat cgtactcatc tgcaaaaccg
     2521 aatttggaat aaaagaaaac atgagtttcc tcctttttat ttctagttca ttcgctttgc
     2581 aaatatacgc ttttattcaa aaaaaacaag ttgctctgta aatttttaca caactcttat
     2641 tttttaccaa agcacttcaa ttgacttttt ctgttattcc cattaaatta aggtctcata
     2701 catatcactt ctatcataaa agggagaata aaaatgaaac aaaccactaa cttgactgaa
     2761 gttcaagaca tactgcaaca gtcatccgtt gccgtcattt atctttcgat gcctaattgc
     2821 agtgtttgtc acgctgttcg tccacgacta gaggaacttc tcacacatta tgatattccg
     2881 gcacttcatt tggatgcatt tgaaacacca gaagtagcta gtaaatttga agtgctgact
     2941 gcgccagttg tcctgatttt tcatcaagga aaagaagtct ttcggcaagc acggttcatc
     3001 gactttaaaa aaatccgtta tctactagat gaactgacag cagaagatga ttccctttct
     3061 tatgaagaaa tcttccgaac aaaataatcg ttgtccaaac agaaaaaagc gattgaacaa
     3121 gtgaaagttt tgccttcatt tgttcaatcg ctttttattt ttattctgct gtattttcgg
     3181 aatgcgcatt tgcgtcagaa tgcaagaaat agatcaaggt ttgcaattca ttcgtcaaat
     3241 caacattttg gatcaataca tctgacggag cattcaatcg agcagcggta aagttcaaga
     3301 ttcctttgat tcctgcttcc gttaactgat tgactacttc ttgtgctctt tcagcgggta
     3361 tcgttaaaat tgctacttcg atttgttgga tcttgatttg ttctgacata tcttccattg
     3421 gataaacggg gataccgtct acgatgcgcc caacgatttc ctcattcaca tcaaaagcac
     3481 aactgatacg tatgctatta ctttgatgga atttgtattt tagtaaagca cttcccaagt
     3541 tccctacacc gatcagcgca acatttgtca attgatcgtc gtttaatgtc tttgcaaaaa
     3601 agtgcataag atcttctaca tcataaccat acccacgttt tcctaattcg ccaaaataag
     3661 aaaaatctcg acggatcgtt gcactatcta cttgaacggc ttcacttaat tcagttgaag
     3721 acactttttt ctttccagca ttatttaaaa tccttaaata gcgataatac aaaggcagtc
     3781 ttttagctgt cgcctttgga ataatatgat ctttcacgaa atgacctcca cgtattgtct
     3841 aattacatct ttgatttctc catcatagca gtatttatag gaaaaaatca attccattgc
     3901 tcagatattg tgaaatttta ttctttattc atttatttat catttctgca aatctcgcag
     3961 tataatggaa tttatggtag actactaatt gaaaaatagt tttttatttg aggagaattc
     4021 acaaagtaga aaagaggatt ccatgatatt attacaagcc aatcagattg ctcgccattt
     4081 tggtgcagaa gtcttatttg aaaacataca tttagaaatc agtacaggcg ctcgtgtcgc
     4141 tttagttggc agaaatggcg ctggaaaatc gactttatta aagatcattg ctggaattga
     4201 agcacctgat gaaggaaaga tcgctaaaaa taaaacagcg actctcggat acctcgctca
     4261 ggacacagga cttgattcca atgaaacagt ttggaacgag atgctgaaag cttttgaaca
     4321 agttagacaa atggaagagc ggatgcgcga gctagagatt ttcattagcg aaggtacgcc
     4381 agacacacct gcttatagtt cttatttaaa agaatacgac cggctgcaac acgatttttc
     4441 agatcgaaac ggctatggtt atgaaaatga aattcgttcc gtattgcatg ggtttaaatt
     4501 cgattcttca ttttatgatc agaaaatcga tactttatca ggcggacagc gcacacgttt
     4561 agctttagct cgcatgcttt tgcaacaccc tgacattttg attctagacg aaccaacgaa
     4621 ccatttagat atcgagacac tttcctggct tgagaattat ctgcaaagct atactggtgc
     4681 attattgatt gtttcccatg accgttattt tttagataaa gtagtcaatg aagtctatga
     4741 attaagcaga agaaaaatgc gccattacaa aggtaactat tcgaaatatc tagatttgaa
     4801 agcagaacag ttagcaagtg aatggaaggc ttatgaaaag cagcaaacag aaatccataa
     4861 attagaagac ttcgttgctc ggaaccttgt tcgcgcctct accacaaaac gagcgcaaag
     4921 ccgcagaaag acattagaaa aaatggaccg gctggatcgt cctcaagggg atgaaaaaca
     4981 agcaaacttt ctattcagta tagaaaagcc ttctggaaat gtagtcttgc aaatcgaaga
     5041 tgccgctatc ggatatgata atgtcacctt atctgaacca atcaatctag atatccgtcg
     5101 tcaagaagcg attgctctag taggtccgaa tggtatcggg aaatcgacgt tgctgaaatc
     5161 aatcattcac cagatccctt ttatccgtgg aaaagaaacg ctagggacaa atgtcaccat
     5221 cggctattac gatcaaggtc aagctgatct gcatacaaac aagacgatcc tagcagaatt
     5281 gtgggatgag catcctacca ctccagaaaa agagatccga agtgctttag gtgctttctt
     5341 attttctggc gaagatgtag aaaaaccaat ccctctgcta agtggcggtg aaaaagcacg
     5401 ggttgcttta gccaagttag cgatgaacaa agaaaatttt ttgattttgg atgaaccaac
     5461 aaaccattta gatattgaca acaaagaagt attagaaaat gcattgatcg attatgacgg
     5521 cacactgctt tttgtttccc atgaccgata tttcatcaat cggatcgcga caaaagtcgt
     5581 cgaactctct gaaaatggca gcaaagtcta tctaggagac tatgattatt atttagagaa
     5641 aaagcaggaa gaagaagaaa tcgccgaatt gttggcacaa gatgagccag aatctgctcc
     5701 tgctcccacg aacaaatctg attattacca aagcaaagaa cagcaaaagc ttatccgtag
     5761 ccttcaacga aaaattactc aggttgaaga agaaatggcg cggatcgatg aatggatcga
     5821 tcagttggaa caagaaatgg tcgcgccaga aaacctggaa gatcacgtaa aactcaatga
     5881 gctgaaccaa gatttagaag ctgctagaca agatcaagaa acaaaattga cggaatggga
     5941 agaactaagc ttggaattag aagaatatga aaattaacga aaaaaccaaa acagaaacca
     6001 ttcatgctgt tttggttttt ctttttttat tttatcaaac aaaaattcca tatttacctt
     6061 tttcaccaat aagaagacca attactagtc attctcacta aagcttctaa ataatagtaa
     6121 tcaccccaaa tacaaggtgt atttacccca ttatttttgg gcttattata aacagactgt
     6181 gttaaaaagc catcttcttc ttccgttgta gcatatcttt ctttcaattg tcgcataatt
     6241 ttccaaacag cagactggta taagttcttt tctgttttgt ctaatagacc tgattctatt
     6301 aattctaata atccacaaac catgatcacc atactagatg tatctttttc ttctctgcta
     6361 gaatcatcaa agatcaaatc ccaataacaa atggtatctt ctggcaaccg attcataaaa
     6421 taattggcta atattttgct taaatcaaaa tatttttgtt ctttcaaata acgataactt
     6481 aatgcaaaac cgtaaatccc ccatgcttgg cctctagccc aacaggaatt atcactataa
     6541 ccttgatgcg tacttccata tcttggtttt ccatctttga catccatata aaatgtatga
     6601 taagttgaac catcttctct tactaaataa tctgctgcat tttgaatatg agcctgagca
     6661 gcttttttat atttttgatt tccagtttct tcactcgccc aatacaataa tggaagattc
     6721 atgcaacaat ccatgatcat tctaccttgt tgtgaaggat cactaggatc tccccaagct
     6781 tgtataactc ctgccttctc ccaaaaacgc tgaagtaatg tttctgcagc atcaattgaa
     6841 gcttccttca tttttcgatt tcccgttatt ttataatctg ctacggttga taaagaatac
     6901 aaaaaaccaa gatcgtgatg attagtgata atttgacagt caatcctgtg gataaaatct
     6961 atatctgctt tttcagctgt ttgttgaaat aaaggatcac ccgtcttctc aaataacaac
     7021 caattaattc ctgtccaaaa tccttctgtc caaccaatat tgtctcttaa tggataacgt
     7081 ccttttatag gacactcgct tgggaattga tactgactgt tactgatacg tttgattaac
     7141 ctctccgtgt gtctgcgaca acgctcgatc tcattattcc attctttttc cattaattct
     7201 ttataacatt catttatcca tttttctgga tgtattatct cttcaaacat cactttatca
     7261 agcatttccc caccagcctt tagcataatt ttagtctttt tttattgttt ttattttgga
     7321 tagttagccc ggttgtccca tcaaaatcaa ttttgggacg attgttccat acttctaaac
     7381 tataatcccg atcttgatga gcaaccactc ctgttccgag ccaatgggtt ccttttccaa
     7441 atgtaccctc taatccagga ataaaagtgt tttcccatgt catcaagttt gtattgggca
     7501 aacccataat agctgtggat tttcgtttaa tagtatcgtc acttaaacag ataatacctg
     7561 aaaagccagc ttcactcacc ctatagtttt ccttgtcttc ttctatgatt attccttctt
     7621 tctcaggaga acattcaata gcaaaccctg tctcatatag aacatattct gtatctaatt
     7681 ctactttgtg tacgcgaatc tgccagcctt caaaaggaat aagccaagtt ctgatttttg
     7741 cctgtaaagt aggttcccat gtagaggcaa gtacctcttt cttacataca atgtctcgta
     7801 cattatttcg aacgatgaac tgtccatagg ttttgatgct ctcattctta aaactggtcg
     7861 tgatatatgt gtccgctcgc tgaatcccta gcattgaatc cattgccatt tctgctttca
     7921 attgattcgc tcgcggtatc gaaaaaccaa attgactaga ataagcaaat tttaaatatt
     7981 tttcattggt caatgtatgg aaatttggcc ctaattgtcc cgcattcaaa aaaaatgtat
     8041 gtccattatc atgttgtatg agcaaattgg cttctgaaag aagtttagtt ccttcacgaa
     8101 taggcatagg catttcttta gccgtccaat actcatggtc ttcaggtagc gccaacaata
     8161 aaaaaatctt attcagccat aatggagata acgtactatt atagggttca gtcaatgtca
     8221 attgagggta ggtataacca atattcagga tacctttttc atcaaatatt tctttttgca
     8281 tccaccatct tatattccga ttgactaacc ccttgatttc acctaattta aaaggcaaaa
     8341 gatcggccca caacatagct gaccaaaagc ttgaaactgc aaaacgataa atcatgcttc
     8401 gaccaaaagg cacatttgca ccatcttccg caaaaaaatg aatatagtct ctagaaaaat
     8461 caatagctcg gttcttatat atctggctaa tgggcttttc tggttctagt acgctataaa
     8521 tcaaaccata ataatgaaat gcaaaaggat tataataatc ttggcgacct cttgaactgt
     8581 ctcgatacca gccattttga agataacaat tttctatatt atcaaatgct tctttcagtg
     8641 ctgattcttt aactggataa ccaaattttt ggataattgt tccgacaatt atcttaaaaa
     8701 attgccaatt accatctgca cattcatatt caaaaatttg cgagaaccaa tgaataatct
     8761 ggcttttttc tcctgtatct aattttttcc ataataattg ttcatgatga tataacgcta
     8821 ataacattgc gggcatttct accatacgct ggtctctttc atgtatttct ccccaataat
     8881 cgggatggga aggatctgtt ccagctttta ttcctgatag aattaatttc aaccatcgtt
     8941 catctaattg gtcaattgcg ggccccgctc cccataaaac ccgagagaat ccttccattc
     9001 ctgcaatttt atttccatat cctacccctt gatgtatata atcaatttgt gcgccacttg
     9061 ctcgataaaa cggctggagt ggttgattta tctttttaaa taagtcaaag taatcctgtc
     9121 tggtccttaa tagtggtact ggatgtttca ttgtattttc tcctcttttt actaccaaat
     9181 agctaattcc gactgttcta atcgaagtaa gccttcaata aaaaaataat cagaatagat
     9241 cagtggcaca ttataagtca gttctctatg gtatgcttct gctccgcctc caatgatccc
     9301 atcttcatcc atattccaat ttgcatagtg cttttctgtc ttttggagta tattgatagc
     9361 agcttcatga tacatttttg atcgtacaga ggaaacatat cctgctaatt ccagaagacc
     9421 gcatgcagcg catactccag cactggtatc aatattcgaa tcttttacag caaaatcaat
     9481 gagaggaata taaccagttt tttcgatttt ctgaataaaa tttactgcta tcttttctgc
     9541 tatagctaga tagtacggat tttttgtgta acgataactt aacgcgaatc catataaagc
     9601 ccatgcattt cctctgctcc aacaagaatt ctcattgtat ccttgcccgc caagtaaccg
     9661 aatatatttc cctgattctg catcaaattc tgcgatatgc ccggttgatc catctttcct
     9721 cactagatac ttagctgttg tatctgcatg ctgccgagca attttagaga acctcggatc
     9781 accagtttcc ctcgaagccc aatacagtaa tggtaaattc atcatactat caataatcat
     9841 ccaaccaggt ttatggtcat tccatgctac taaatagttc cctgaaatat tatagcgtcc
     9901 agcaagtacc gtagctgcat gtaaccctag ttgtcgacta tgtttgtttt gagactgttg
     9961 ataatgtgca atagccgatg gtaagaataa aaaacctaca tcatgatgaa cgtctgaaaa
    10021 atggtacagg gcagattcta ataattgttc ttgttgtatt gctgcctctt tgaacttttc
    10081 tcgttttgtc ttgtgaaaga gctgccataa aataccactc caaaaaccat tggtccacca
    10141 gtcgattcct ctgtccttta ttacgtctgt atattttccg ttgtaagaaa tatatggaat
    10201 tttattcccc attctgtcta attccgcatc tatcttttca gaagctacat tcaaaatttt
    10261 tcttgaccat tccttcgtct gttcattcat ggatatcccg ccattctttt aatttttatg
    10321 aatgtggagt tgctcctaga acgaactaga tggttaacat tcgttatttt ttttacttat
    10381 ttgcagctcc aatgtcattc tccttaaatt tagactgcgc ccacacataa atcaagacca
    10441 ttgggatcac tgaagttgct aaaattagca taattgcgcc ataattaatt ggctcacctg
    10501 tactagaaac cgtcatattt ttgataatta agggaaccgt atatttatct ttatctgaaa
    10561 gcatgactaa tggaaataga tattgattcc aacttcccat aaagataagt aaaccactag
    10621 taataatcgt aggtgataaa atcggtacag cgattttcgt aaaaatcacc cactcattgg
    10681 caccatcgat tctggcagcc tccatcagtg ctgtaggaaa ttgactgcta tattgtcgca
    10741 tcataaacac tgaagttgta ctggcaatcg aagggatgat cacacctgca taagtattcg
    10801 ataatcctaa acgactcata atgtaaaata aaggaatcaa tgtggtaaaa aaaggcacca
    10861 tcctagatag cataactgta ttgaacaaca gtttattccc cataaaacga tactttgcta
    10921 atgcataacc cgccattgta gaactggctg tagctgaaac tgtactgatt acagcaataa
    10981 caatcgaatt ccaaataatc aagggataat tatatctcgc catgatggct tgccagtttt
    11041 ttaatagcat atccccaaac ttgaaattga ttccgccact taaaatttct tgattacttt
    11101 gcgttgaagc tacaatcata tataaatatg gcatcaaaaa tattaaggca ccaaacacaa
    11161 taatgacgta cgtaaaaatt ctgctttgta tcttttgttt agtcattttt atcacctccg
    11221 aatttaaatt gaatagccga taatataaca gatacaaaca ccatcgtcca ggcaatagca
    11281 gaggcttttc caaaatcaac catttcaaaa cttgttttat ataaattgac tcctacgacc
    11341 aaagctgaat tttctggtcc attggaagtc ccaaagaata tatttgggat attgaaatta
    11401 cccaagccat tgatagtggc taaaactgtg gccattaaaa tgattggctt tagcatgggg
    11461 atcgttaata aaaagaattg ccgaaatttc gatgctccat ctatttcagc actttcgtat
    11521 atctctttag aaatcccttg aagaccagct aaaaataaaa tagtgttata tcctacacta
    11581 agccaaatca tcgtgatgat cattgatatt cgtatccaaa aaggatttgt taaccactgt
    11641 acaccgttaa ttcctagcgc attcaaaaat ccattgaata acccagtcgg attgaaaaaa
    11701 gtcatgaata cggaagctac agccaccgtt gatgtaatgg ttggaatata atagacagtt
    11761 ctaaaaaatc cttttccttt taactgtgct gaattcaata aaacagcaaa gtaaatagct
    11821 aatagcatcg ttattggaat tgatcctgcc atgataatca aaacattcgt gtaagattta
    11881 ataaacaatg gatctcggaa gatagctaga taattgtcaa atccaacaaa ttcaaatgag
    11941 tagccaattt tcataaaact aaaagcaaac gagataatga tgggaatcaa aatcgtcaca
    12001 ataaagaaaa taacaaaagg tgtgaccaat cca
//
LOCUS       DACEZN010000079        12015 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEZN010000079 DACEZN010000000
VERSION     DACEZN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.079"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            708..1679
                     /locus_tag="IXH60_001702"
     CDS             708..1679
                     /locus_tag="IXH60_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1990623.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1757..1993)
                     /locus_tag="IXH60_001703"
     CDS             complement(1757..1993)
                     /locus_tag="IXH60_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1990624.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1962..2093)
                     /locus_tag="IXH60_001704"
     CDS             complement(1962..2093)
                     /locus_tag="IXH60_001704"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1990625.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2169..2552)
                     /locus_tag="IXH60_001705"
     CDS             complement(2169..2552)
                     /locus_tag="IXH60_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1990626.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2585..3370)
                     /locus_tag="IXH60_001706"
     CDS             complement(2585..3370)
                     /locus_tag="IXH60_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1990627.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3548..4648
                     /locus_tag="IXH60_001707"
     CDS             3548..4648
                     /locus_tag="IXH60_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1990628.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4638..5462
                     /locus_tag="IXH60_001708"
     CDS             4638..5462
                     /locus_tag="IXH60_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1990629.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5468..6274
                     /locus_tag="IXH60_001709"
     CDS             5468..6274
                     /locus_tag="IXH60_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1990630.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6271..7101
                     /locus_tag="IXH60_001710"
     CDS             6271..7101
                     /locus_tag="IXH60_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990631.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7108..8139
                     /locus_tag="IXH60_001711"
     CDS             7108..8139
                     /locus_tag="IXH60_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990632.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8246..9519)
                     /locus_tag="IXH60_001712"
                     /pseudo
     CDS             complement(8246..9519)
                     /locus_tag="IXH60_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9796..10005
                     /locus_tag="IXH60_001713"
     CDS             9796..10005
                     /locus_tag="IXH60_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990633.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            10055..10771
                     /locus_tag="IXH60_001714"
     CDS             10055..10771
                     /locus_tag="IXH60_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1990634.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10673..11193)
                     /locus_tag="IXH60_001715"
                     /pseudo
     CDS             complement(10673..11193)
                     /locus_tag="IXH60_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11481..>12015
                     /locus_tag="IXH60_001716"
     CDS             11481..>12015
                     /locus_tag="IXH60_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1990635.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKD"
BASE COUNT         3452 a         2223 c         2731 g         3609 t
ORIGIN      
        1 tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga catcatagct
       61 atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt agacatcatc
      121 cataaaatgg actttgtcta cagtctggat aacgtgattt tttgctgtaa tgacaaggtt
      181 ttagctgttt cagataccat gtatccaaaa atagtttatt gagaaaatag gagagtattt
      241 gatactaact agtaggaaaa aatgagataa aaagtaaatg gaatcattta aggtaatctg
      301 aaataaaaaa tatacagccc tgtggtactt tacctacaag gctgtatata ttttttactc
      361 tcagcgtttt tgactccgtg tctgactccg ttttattgat tttgaatgtt attcaatgac
      421 catttaacag gtcaaatttg atgtttttga ttccgtatga cgccgtttga tatctagtga
      481 tatagtgatt tgagtctaca tcgaataagg gaataaacca ataaaaaatg atttagaata
      541 ttaaaataac aaaaaagagg actacctgta ttagtcctct tttttgacca gattttgacc
      601 agtctatttt acatcctagt atatttatat gagctaattt tatgattaaa tggtaagttt
      661 ttccttgtgt gttttagttt aaactagtgt gtaattcaaa aatctatatg gaaggaacag
      721 tattaagaat agaacagggt tctctacatg atggtgctgg tttacggacc gttgtttatt
      781 taaagggctg cccattacgc tgtgcctggt gttcgattcc cgaatcacaa agcaagcaga
      841 ttgaaaaagg atttggccag accatgaccg ctgaagaggt aatggatgag attgagaagg
      901 atgctgtttt ttactttcac tcggatggcg gagtgaccat tagcggaggc gaggcactgg
      961 ttcaggcgga ttttgctaaa gaaattttgc aaaaatcgaa atacattgga atcaataccg
     1021 tactggaaac cagcttttgt ggtgcttata atgaaatcca aaaggtagct ccctatgtgg
     1081 atacattgtt tgtggatgta aaaatgttta ccagcaagct ccacaaacaa tggaccggct
     1141 tagataacca acaaattttg aagaatattc gtcgcttttt aattgagtat cctaactgcg
     1201 aagtgcgaat tcgagttcct gtggttccag gtatcaatat gaacctgacc gagctgctga
     1261 cgattgcttg ttttgtggca gatcttgatc ggttcgtacc gttagaatta ctaccgtacc
     1321 actgctatgg tatgcacggt taccaagcat tgggactgga gtatccgttg gcagacacgc
     1381 cagcgccttc acctaaagaa atgtttgcat tggcagatca actggctcgc acggttcctc
     1441 atttgctggt cacagcttta accaaaacat ttatttattt attgaatcca agctttatct
     1501 tatggataaa tgaaagacac tcactagctg cagccagctc aagaatagag cttttgctga
     1561 agtcagtgag tgtcttttta cgatgttttt tcgaagttaa tttgtcgaat gaacactggc
     1621 tcaatcggta catcttcact gtttgtcggc tgagctgcga tcgctgctac gatttctaac
     1681 ccttggtata cctgtccaaa aacagtataa cgaccatcca gctccggtag tcctccaagc
     1741 tccaagtatg cttgctttac attagcaggt cagcctgcgc ctttgtgttt tgcacaataa
     1801 aaaactggtt gccgttggtg tgggggccgt ggtcccctaa ggccaaggcg ccattaaaat
     1861 gataaagtcc gcgatgcact tcatcagcga aaaagccctc atagctggaa acagcttcat
     1921 caccagaacc atccaaagcg ccaccttgaa taacaaattc cttaatgaca cgagccgaaa
     1981 ggtgtgttgt cataaacgcc ttgctcagct agggagcgcc agttggctac agccagggga
     2041 gcttggtcag gaaataagcg gagacgaatt tcaccagcat cggtgaccag cacacaaact
     2101 tcttcctgct gcggtttttc taattgaaag agaggaaggt ttgcaactga aacgccttga
     2161 tcctgccatc aaatatcgaa aatccgccac tcgaccttcg cctgatcacc aaggcgagcc
     2221 attcccaata aaacggtata ggcggtttca tccgcttgaa aggtcaattg cagctgtcct
     2281 cgtaaatcgt tgtagtcatc tggggcggct acttgataaa aaagatgaaa ctgcgcatgc
     2341 aaatattcca aaggctgatc tttaaaggct gcaaaataag cctgaacttt ttcccgctgc
     2401 aagctgtcag ccaccaaaaa attttccagt tgttgttttg gaacggctgc taacaaggat
     2461 cggcaaaccg cctcaatttt caacgcatcc caatgtcgcg caattaaata atggatgggt
     2521 gcctcttgat tgtagtccca caggttgctc atttttttca ccactttctt ctattatata
     2581 ggaatcacaa aatcggcagc ggcgtcaggc ggcgttcttt tccttgaatg actagcaact
     2641 tttctacctg acagtctgcc aacagctgac tcattttttt aaagcccagc tgataatctt
     2701 cggcaacatg agcatccgag cctaaggtaa agagctttcc gccgacactt tgatacaaag
     2761 gaacggcata acggtaaagg tcttcgttgc cgtatttcaa gaagctcttg gcatttaact
     2821 ccatcgccaa ttctcgttca acaacctttt tgaaaatcgt tagcagctga gcctcaaaat
     2881 gctccgcgaa ttcttccgct gtgaaggtga agcggcgtaa tccataatca aagtgagtca
     2941 aaatatctcc ttctggaaaa tccgtcaaga cctgcaacat ctgatcaaaa tattccttgg
     3001 tagtttgaat gggatctttg gttaaaacca catcgtccat ataatcaaag cggccatttt
     3061 gatgaatact taccagcttt aaatcataag gatgctgagc gaggtaatcc acgatccgct
     3121 cttcttgtcc tggaacaacg ccaatctcga tacctcgcag aaatttggtg gaataggttt
     3181 cttttaaaga gtccagtttg tttatatagg ctggataatc cggaatgtca tcttttccgg
     3241 tagatggatt gtttaaatca aagtgatccg tagaaacgaa aaactccggc tgataggcta
     3301 aatagttttc aaatttttcc tctgaatcaa aggaaaaata cgtgtggaga tgctgatcat
     3361 aatactgcat agggagcctc cttttttcta ctatcatacc gcaaaagctt tagaaggtta
     3421 acgggaattc gtcgaaattt acaaaccata tacaaccttt acattgttca tgaaaccttt
     3481 tcaagaaact gattctctca ttacaaagct ttgttagaat ggaattatcg aaaggggaga
     3541 agaatgaatg aaaatgaatg aaaaaattcg taacatgact atcggtttat tttgtgcagc
     3601 aacagtaagt atcctgctga caggctgcgg ctcaggtgaa gcaacgaagc aatcggctgc
     3661 ggcagacgac tctttagata cgattataga aaaagccaag gaagaaggag agattgccag
     3721 cttgggaatg ccggatactt gggcaaattg ggtaggcact tggaacgacc ttgaaaaaga
     3781 atacggctta agtcacgtgg atacggatat gtccagtgca gaagaattgg ctaagtttga
     3841 atcagaagga aaaaatggaa ccgccgatat tggtgatgtc ggtatcagct tcggtccgtt
     3901 ggctgaaagc aaagagttaa ccttacctta taagaccaac tattgggagg aaattccgga
     3961 ttgggcaaag gatgatgatg gggattggtt gttaagctat acgggaacca tttcctttat
     4021 caccgataaa gagaatgtga agcaagcacc gactagctgg aaggagctgc aagagggtga
     4081 ctatcaagtt agcgtggggg atgtgacgat tgccaaccaa gcacaatttg ctgtattggc
     4141 tgcggcgatt gccaacggag gtgacgaaaa ggatattcag ccaggtctga attactacaa
     4201 aaaaatggca gaaaaaggcc gtttgtccac cgttgatgcc agcgtagcaa atcttgaaaa
     4261 aggtgaaatc gatgtggcag ttgtttggga cttcaatgga ctaaattatc gtgaccaaat
     4321 tgactccgag cgttttgagg taactgtgcc aacagacggc tccatcatta gcgggtatac
     4381 aaccttaatc aataaaaatg cccctcatcc caatgcggca aagctagcac gggaatatat
     4441 tttatctgat gaagggcaaa tcaatttggc aaagggctat gcgcgaccta ttcgcaaggt
     4501 agagctgcct aaagaagtag ctgaaaaatt actgccggag gatgcttatg aagcggcggt
     4561 gccagttgaa gacaatgaag cttggaacaa gacctctgta gagcttccag agctttggca
     4621 ggcggaggta ctgacctatg caaagtagac ttgacaaact gattgtagtt atactagacg
     4681 gctgtcgcta cgacacagcc gtcgcccagc ttgggttcat gaatcattta gttgagcacc
     4741 agcaagcaag cttattgaag gtaaaaagtg agctgcccag caattcacgt ccgttatacg
     4801 aagtactgat gaccggcgtt ccagcctatg aaaatggcat ctatagtaat taccatgctc
     4861 agcgatccaa agagtggtcg ctgtttgaat tagttaagga agctggcgga aaaacaggag
     4921 ccgccgctta ccactggtac agcgagttgt acaatcaagc cccctataat cgtttggttg
     4981 atcgcatcca gcatcaggag gagcgactga ttcagcatgg catcttttat agtgaggaca
     5041 cttaccctga ttcgcattta tttgcggatg cccattattt agtgaatcag tatcagccag
     5101 actttttggt cgtacattcg atgaatattg atgatgtcgg tcacaaattt accgccgatt
     5161 ctcatgaata tgcagcagcg gttaatcagg cggacagatt attggctatt taccttcctg
     5221 aatggctgca aaaaggttat caagtcgtgg tgactgcgga tcacgggatg gatgcgcacg
     5281 gactgcatgg cggcagcttg gctgcacatc gtgaagttcc tttttatttg atttcaaata
     5341 aacgccaaaa gctgaaggaa caaactgtac cacagctgct ggcggctcca ttgttttgct
     5401 ggttattagg aattgcaccg tcagagaaaa tgcaggacat tcaagaattg atgaggggat
     5461 gaaagagatg aaaaaacgct tggttctggt tcctgcctcc tttatcttct gtgcattttt
     5521 actgctgccg ctgcttttta tggttctgtg cagcttgcac agtgatttaa ctaaccagtg
     5581 gacattggcc aattacagtg atattttcag caacaaatat tacacacagg cctttgttaa
     5641 cagcgtactg attgctgtca gctccagtgt gattgggtta atcgggacgt ttattttatg
     5701 tttatgctta atgaatctat cgactaaatt gcaggagaaa atcacctttg tttccaattt
     5761 ggcagctaat tttgctggga ttccgctggc attctctttt attattttgt tggggaatgt
     5821 aggtgtgctg aaaatgattt tgccatttct agcggacttc aatttgtatt cttggtgggg
     5881 attgaccatc acgtatacct atttccaaat tccgttaggg gtcttgtttt tgtatcccag
     5941 catgaaggaa gtgaaaaagg aatggctgga gtccgcacaa ttgctgggcg cttcatcagc
     6001 gtatgcttgg ttcaaggtgg ctttgccttt tttgcgacca tcgattgcca gcacctttgt
     6061 catcttattt gctaatggca tggggaccta tgaaacagcg tatgccctga ccagcagtaa
     6121 tgtaaatctt ttaacgattc gaattgcggc tttagtgtcg ggagatgtgt tcgccaagcc
     6181 taatttaggc agtgcgttgg cagtcgcttt aggtgtcctg ctggtaacgg caatgttgat
     6241 tattcaaaag aaaggcaagg tggtgcagac gtgaaaaagt taataaaacc gattttgttc
     6301 tgctttgtcc tgtattactt gctgccgatt attggaacga ttttgtatgc cagctccact
     6361 aaatggtcaa aatcgctttt acctagtgat tttaccttgc aatggttcca acagctgctg
     6421 acggacaggg aatttattgc tgcggtgggt cgctcacttt tattagcagg tgtggtcttg
     6481 gtgacgattc tgttgttgat gattcccacc attatttgga ttcatttgta tttccctcgt
     6541 ttgaatcgtt ggctggaaaa gctgctcctg ctgccgtatg ctttaccagg agtgattttg
     6601 gtaacggctt tgcttcgaac gtatgctgaa actggtatcc caatgtttgt agtgttggta
     6661 ggtgcattgt ttattacggc gttgccgatt gtctatctta gtcttaacaa ccagatgcgc
     6721 ttaattaatc tgaaggaatt agtggatgca gcagaaacct tgggcgcccc gatgtctacg
     6781 attattatcc aggtgctgtt tccaaacatc cgtatcggtg tgaccctagt ttctctgatg
     6841 attttttctt cggtttttgg ggagtacatg ctgacgaacc tgttgattgg cggtcgattt
     6901 cagacggtac gcatctatat gctgcggcgg atgaacgaaa acggccattt ggctagcgct
     6961 gtgatggttt tgtatttgct gtttatggtg ggaattgctt taactacctt tatgctgact
     7021 tatcgacaaa agcaacagct gtcccttaag cagacgaggg aagagaagca gtcgaaaaag
     7081 aagcccttaa aggagttgtg acggaaaatg tttttagaaa tacatgatct caatctgttt
     7141 tttgatgaaa aacagatttt agctattgat gaaattcaga ttgaaaaggg tgagctggtt
     7201 actttactag gtcccagcgg gtgcggaaag tctacgttgc tgcgctgtat cacgggattg
     7261 gaacaaccaa agagcggtga gattgtgctg gacaatgaga atattgccga taagcctacg
     7321 aaggatcgca atatcggctt tgtttttcaa cagtatgcat tgtttccaac tatgactgtt
     7381 tttgaaaatg tagcctttgg tttgaaggta aagaagctga gtcctgaggt gattcaggaa
     7441 aaggtttttg agatgctatc attggtggat atgacggagc aggcagataa aaatgtccag
     7501 tttctttctg gcggtcaacg acaacgggtg gcactggctc gctcattggt cactgaaccg
     7561 aaagtcctgc tgctggatga gccattaagt gctttggatg cccgaattag aaaacagctg
     7621 cagcgggatt tgcgcgctat ccagcaatcg ctagggatga cgatgatttt tgtgactcat
     7681 gaccaagaag aggcgatgcg aatcagtgat cggatttttg tgatggaagc tgggcgggtg
     7741 gctcaggttt ccacctcgaa ggaattgtat cagaatcctc agagccgatt tgttgcggaa
     7801 tttatcggca actataatcg tttcgaatgg tatgagctga atcagtatac ccacgagttt
     7861 tccgctaagg gcaatcgctg catttattat ttaagaccgg agctgattca atttgaaccg
     7921 gttcctcagg gggttaagat tccggttcta tggaaggaat catttatttt aggcaacatt
     7981 caacggtatg tttttcaaac gctggagggc aaagaaattt tagtggatcg gttgaacgat
     8041 cgggaagaga aaattagcga tgccttatac attcagctga aagatattct agcaatccct
     8101 gtggaaattg atactcagca ttttgcacaa caaagctagt gtatagtcgg caaacatttc
     8161 tggctctttg tcaaatagga ctgatagagt gatataagaa catctaaggt agaattgatt
     8221 ttctgcttta gatgtttttt tatgtttaat aagtgatcag atgattgatt aagaaaattc
     8281 tggttctcat cctaacccgt aagctattcg tttcaatgct ttgatgtggg tatgcagatt
     8341 ttctattttg ccatttgagt aaggcaactc taaggctaaa cgaatgccgg attcatagtt
     8401 tataaacgct ttttttatat gatgcagttc tcttggaacc gacttgggca ttgtttttac
     8461 aagctgaaag aagatgtccg cttctttgtc ataaaaagct tgaagaattt cttgatagta
     8521 agcataagct attcgtagtt catcagaagt agaaagtaga cgttctacga cttcactttc
     8581 ggttaaatag gcccaaccaa aattacgcca ttttgtccat ttatcataaa caagcgtgtc
     8641 ctgtcgtttg atcagaagtc gagataaagc cttcacttgt cgatagtgtt tggcttgtat
     8701 tggatcgttt cggtcgaagc ttttcatgac tcatagtcaa atatccttaa aactgcgaat
     8761 gaggtgcttg acgatgtgaa agcgatccac aacgagtttg gcatgaggaa agcactcttt
     8821 tatcagcaaa gcataagagg cgttcatatc tgttacgagt atttttacgt ttaaacgtgc
     8881 acgtcgttca aattgcataa aataagaaat gagatctttc ttcttacgtg aaggaagaat
     8941 atccagtaat ttcccagtat cgccatccat acaagaaaaa gaatagggac cgacttgatt
     9001 tttcaatgaa cgaaattcat ctaccaatga acagcgaggc aaccaattta ttttcacgtt
     9061 tgtcatgggg agcagagaag cgagtgttcg cgaaacggtt gaccaagaaa cgccgcaatg
     9121 tttagaaatc gtcgtcattg cttgtttttc agtcaattcc tccaatattt tataatgtac
     9181 ttgtttggcg ataaaacagt ttttttcaac aatgtaggta ttcgccgtaa aataggtgtg
     9241 acagccacga cagaggtacc gttgctttgc caaagcaaga taagtccgtt gattcgcaca
     9301 cttattgaga aggaccagcg attttttctt tccgttctta acgatcagcc catgttgatt
     9361 cacctgttta cactgaggac agggctgaat cgttttaatg agcgtggcag aaacaacgat
     9421 actgttgatt gaatgtattt ttttcttctc gacatgagtg atttgaatgt ttccatcttt
     9481 gattcctagt aatagtttga taagatgaag atcaggcata cagaacgctc ctttgattgt
     9541 gttgtggtga ttcaattcta acggagcttc tgtatgtttt gtttttttat gctcaaaagg
     9601 actgatagaa tgatttacta tcagtcctag aaattatagc gccacatttc tttcaccttt
     9661 gacttttcag acggcagttt ttatgctgat actgtagaga gagcaggaca cagcttttgc
     9721 ttgttttggg taagggggaa agaagatgac caaagcactt atttttgatt ttgatgggac
     9781 gatgattgac agtgaatgga tgttttacga gagtattcat cactatttac tgaagcctat
     9841 ttactggagc agttcggcta taaaattcct tttgcagact atcaacaagg tgttgggacg
     9901 gcagcagaac tgtcctttta tgaagggata agtcaagcgg ttgggagacc agtggatgct
     9961 gaacattttg agcagcggat cagcagcact ggaaaatcgg agtagtctca attctgaact
    10021 gcgggagcta gactattttt ttgaagcaca tccgatgatt aaggaaaaaa ttgattcctt
    10081 tgtgacgatc gatgatcttc aggtcggcaa accagatcca gaaggttatt tgttgtgctt
    10141 gaaacggctg agggttgaac aggctgttgt agttgaggat agtccagtcg gtgcccaagc
    10201 agcaccgcat atgacagttg atgtacaagc gttggatgca gattttttcg cctttagcgg
    10261 gcataaaatg tgcgggccga caggaatcgg tgtattatat ggcaaacgtc aattgttaga
    10321 acaaatggag ccagtcgaat tcggcggtga aatgatagat tttgtcaatc tttacgatag
    10381 ttcatggaaa gagctgccat ggaaatttga agctggtact ccgaacattg caggagcaat
    10441 cgattacttg aatgagattg gtatggagaa tattcatcgt tatgagcaag aactggttgc
    10501 ctacgtttta cctaaattac aagcgatcgg cggtatcacc atttacggac cgcaagatcc
    10561 tgaacatcat actggcgtgg ttgcttttaa tttggatggc atccatcccc gtgatgtggc
    10621 gactgctctg gatatggaag gtgtcgctgt acgttcagga caccattgtg cacaacggtt
    10681 catcgacgat ttctgtttga ctaattggtc gtttaaacag tcgatgataa tgccgtttga
    10741 gcggctccaa tgtcgtggaa tctttgagta agagtttcca atatcgcttg agccgacggt
    10801 acttcttctg attttcagat tacgtgattg attttcgtac agttctatga gaatcgcctg
    10861 cttcagttcc tttgttagtt gatgatgttc atcaactaag tccgttttcg cagaaaaggt
    10921 ctgcccacaa tcatagcata gaaaacgggt acgttttaat tctaaaatag tcaacttttc
    10981 tttgactttc aacatctgag tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga
    11041 tccacagtgt tcacaatggg aaggtgagtc atccaatctc cctttaataa actgcgcttg
    11101 aacgccttta tattcttttt caatcagcca ttcttcatca aaaaataaat gtttatctgt
    11161 taatccgagt aattttctag tatgattatc catagagcga ctcctttggt agggtgatgt
    11221 tttggtcgac taaattttac caaaagtgag tctctttttg agttgaaacg aagaaaaagt
    11281 gttagtagaa aatcgccttc gattttccaa caacacaaaa tattatagaa gccttttttc
    11341 taaaggattt caaggtgtct gtttgcattt attaaaaaaa agaaaaagta ttggcaacag
    11401 aaggcgtcac caacactaga tattatagag ccataatatg ctgtataata tctagtgtat
    11461 aggcttaagt ggaggtatat atgaaaatat taactgttga agatgataat atgataagag
    11521 agggaataag tgaatatctt tcagaatttg gatatactgt tattcaagct aaagatggaa
    11581 gagaagcctt gtcaaaattt aatagcgata taaatttggt tatcttagac attcagatac
    11641 cttttataaa tggtttagaa gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc
    11701 taatcttgac tgcatttagt gatgaagaat ataaaattga tgcatttact aatttagtag
    11761 acggatatgt agaaaagcca ttttcattgc cggtcttaaa agctagaata gattctttaa
    11821 ttaagaagaa ttatggacat ttagagaagt ttgaatataa ggatctatcg gttaatttta
    11881 atagctatac agctaaaata aatggtgaag aaatagatgt aaatgcaaaa gaactagaag
    11941 tattaaaatg tttattggat aataatggac aagtgttaac aagaatgcaa attattgatt
    12001 atgtatggaa agata
//
LOCUS       DACEZN010000080        11658 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEZN010000080 DACEZN010000000
VERSION     DACEZN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.080"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..258)
                     /locus_tag="IXH60_001717"
     CDS             complement(<1..258)
                     /locus_tag="IXH60_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990636.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIYFAE"
     gene            complement(263..607)
                     /locus_tag="IXH60_001718"
     CDS             complement(263..607)
                     /locus_tag="IXH60_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1990637.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(867..1514)
                     /locus_tag="IXH60_001719"
     CDS             complement(867..1514)
                     /locus_tag="IXH60_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990638.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            1736..1915
                     /locus_tag="IXH60_001720"
     CDS             1736..1915
                     /locus_tag="IXH60_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAQ1990639.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            complement(1908..2249)
                     /locus_tag="IXH60_001721"
     CDS             complement(1908..2249)
                     /locus_tag="IXH60_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990640.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(2273..2509)
                     /locus_tag="IXH60_001722"
     CDS             complement(2273..2509)
                     /locus_tag="IXH60_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990641.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            complement(2784..3251)
                     /locus_tag="IXH60_001723"
     CDS             complement(2784..3251)
                     /locus_tag="IXH60_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAQ1990642.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(3326..3415)
                     /locus_tag="IXH60_001724"
                     /pseudo
     CDS             complement(3326..3415)
                     /locus_tag="IXH60_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(3436..3681)
                     /locus_tag="IXH60_001725"
     CDS             complement(3436..3681)
                     /locus_tag="IXH60_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990643.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(3641..3997)
                     /locus_tag="IXH60_001726"
     CDS             complement(3641..3997)
                     /locus_tag="IXH60_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1990644.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(3997..4302)
                     /locus_tag="IXH60_001727"
     CDS             complement(3997..4302)
                     /locus_tag="IXH60_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990645.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(4299..4460)
                     /locus_tag="IXH60_001728"
     CDS             complement(4299..4460)
                     /locus_tag="IXH60_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1990646.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(4475..4834)
                     /locus_tag="IXH60_001729"
     CDS             complement(4475..4834)
                     /locus_tag="IXH60_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1990647.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(4831..5682)
                     /locus_tag="IXH60_001730"
                     /pseudo
     CDS             complement(4831..5682)
                     /locus_tag="IXH60_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5699..6529)
                     /locus_tag="IXH60_001731"
     CDS             complement(5699..6529)
                     /locus_tag="IXH60_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1990648.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(6532..7218)
                     /locus_tag="IXH60_001732"
     CDS             complement(6532..7218)
                     /locus_tag="IXH60_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990649.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(7224..7463)
                     /locus_tag="IXH60_001733"
     CDS             complement(7224..7463)
                     /locus_tag="IXH60_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990650.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            complement(7789..7977)
                     /locus_tag="IXH60_001734"
     CDS             complement(7789..7977)
                     /locus_tag="IXH60_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990651.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(7998..8282)
                     /locus_tag="IXH60_001735"
     CDS             complement(7998..8282)
                     /locus_tag="IXH60_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990652.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(8284..8478)
                     /locus_tag="IXH60_001736"
     CDS             complement(8284..8478)
                     /locus_tag="IXH60_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1990653.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            complement(8475..9251)
                     /locus_tag="IXH60_001737"
     CDS             complement(8475..9251)
                     /locus_tag="IXH60_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1990654.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            complement(9378..9599)
                     /locus_tag="IXH60_001738"
     CDS             complement(9378..9599)
                     /locus_tag="IXH60_001738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990655.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            9900..10244
                     /locus_tag="IXH60_001739"
     CDS             9900..10244
                     /locus_tag="IXH60_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1990656.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            10280..10675
                     /locus_tag="IXH60_001740"
     CDS             10280..10675
                     /locus_tag="IXH60_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1990657.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            10728..11039
                     /locus_tag="IXH60_001741"
     CDS             10728..11039
                     /locus_tag="IXH60_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990658.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            11051..>11658
                     /locus_tag="IXH60_001742"
     CDS             11051..>11658
                     /locus_tag="IXH60_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1990659.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYT"
BASE COUNT         3405 a         2257 c         1758 g         4238 t
ORIGIN      
        1 ttcagcgaag taaatgtaat aattaatctc ttcaatacta tatccaaaaa cattttttat
       61 tctttcgacg ttattatctt tattcaacgc ttctcttact tgattcactt tgtatttact
      121 acaacagtta tctgataaca gatccctaat acctacatag cgaacgtata tcaaacgttt
      181 aattaatccc tgagtataag atgaatactc ttcaatcttt tctgtatcat actctctgcc
      241 attgtcattg atgatcatgc acttaccacc tttcacttgc atcgaagtta gcgaagcttt
      301 ctatcttctt ctcttgttta tctaacgctg taagatatct gccatgaact tcattatgat
      361 gttcaacaca taaacaaata agattatcta aatctaaagc taagtcaggt ctatccttga
      421 cttcctttat atgatgaacg ttctctactc tatgatactt acctagtctt ctacactctt
      481 ggcattcata gtgatctcgt ttcatcgctt tctctctaag cctgcgccat ttaggagact
      541 gatagaactt aaccaaacga tcttctctta tcaactgtaa taaccatcta tagaattcct
      601 cggtcatgtc ccatctcctt tcgcaatctt atttaatact tagctattct tttgccatac
      661 aatggaataa cttcattgct ttccttccgt ttatatgtat cgctctttat tggtcttcta
      721 tactttcgta ctatctcacc gttaccgttt tgcacagtga ttacttcatg cttctgttct
      781 aagtattgtg gtctatacat cgtcgtgcct cctttacgca aaataaaaag accactcaaa
      841 gagtgatcta cgaattttaa aaaatctcat actgaaatct tatttttaat gtctttatat
      901 aagttcgagc ctcgcaaaac ataaatatct tgataatatt ctttgccatt atattctaag
      961 aataaaaagg aaaatttcaa ttttttacat tttttatatc tcttttctaa tttcttgctg
     1021 ttctttatgt tagctaccat tgcagtaact aaactttcat ttacaattgg tatttttaga
     1081 cctttttcta agtatccaat attctggaat tcttgattaa taaggtctgt tggattgaga
     1141 taagatattt cgtggtccac atatttttca ataagctttt taactacagg gtatttgtaa
     1201 atatatttgt accaatgctt tctgataaaa cgttggaaaa tgttatattt acctataatc
     1261 ccgcaaaact ttattgcggt ttcagatttc ccttcattta ataaagttat agtaaaccca
     1321 tatttaccga cgacagtaat atcttttttg gttattcctt cagttattct tgatacaatt
     1381 ttagaactat tagaatacca gtagtacttt gatgtttgtt gaattgtaat cactatcaaa
     1441 ataataccta cccattcgcc taacgtacca tatccatcaa atgtaaaccc aagaaattta
     1501 actacgccca ccattttcta tcacctcaga aataatatta tcaaataatc ttagataact
     1561 aaacagggga cttgtaaatt tcagataatt actcattgac ttttcccatt catcctttat
     1621 tgcgtctacc ctttccgcca cagcgacaaa ttgatattgt gaaattttat acatagctta
     1681 taattttaat tatcagcgag tggtccgctg aaataaatta taggtggtga aaaatttgta
     1741 ctttgttata agagaagcaa ctaatggcca atattacttc gttattaagt ccaataacaa
     1801 tgaagtagta gcaactagcg aaacatactt gactaaatat tccgctgaac aaaccattaa
     1861 ttctataaaa aacggaatca ccaaagactc tcaagttatc gatatgacta aataagacga
     1921 ctaacttcat ttgctaggga gttcatttca gctgcttttt ctaccaaagc agttgagata
     1981 ctttctagct tttctgattt aacagaatta aatactggcg tcttgatact aatcgataca
     2041 tcttgacgag aatcaggact cgttgctcta tttttttgca ccactgatct gtacttttct
     2101 actgaatgat actcaattac ttgttccaaa tgttttttgt actctttaca tacagcttct
     2161 ttttggatca attctaaacg ttgttgctca ataatatcaa tcaaccgttc ccgatccata
     2221 ccttgatact ttatttgttc gacttccatt catttcccct ccaatacata aattaattag
     2281 ctaattgttg ctcattgatc tggagagcta tttcttcttc tataagctca acgatcttcc
     2341 tttttgtcga atttaattcg aacttatgat ccattaaagt aagctttatg ttcttaatct
     2401 tttcaccttc gaattctttg ataatatctt cgattctttg gtttactaaa attgttagtt
     2461 gatcaatttt ttcggaagcg atcttcattt tatataccct ccaatacata aattaataga
     2521 cagcaacgga tgatagataa taagaacaat ttagaaggag ttgaaattca catccttatt
     2581 cttaatattt ccgctgctgc ctatcgaagc ttaattaaac gatgagggag atttcctccc
     2641 ttacatttta ttttgtcgat cctgtttcct aatctttcga cactatcata atacaacgtt
     2701 gaataggtaa gtgattggta taaaaaaggt ataaaatgga aaccaaattg gtaataaaag
     2761 ggtataaaaa gtgtaaaaac tggctacttg aaagcaacca gttctaacga tgaagcaaat
     2821 tggatgataa ttctgtttga ttctacttta accgattctt cgctagtatt attcctttga
     2881 gcagttacat aaatgggcag accattgata taacgatcat agaatatctt cttgcgcctt
     2941 tcagtcacat caggcttatg cggatgctgt atcgctgaat agcctcgaac aaacaattta
     3001 tgcagatact caaattcttc ctgtgcttct tctttatcga tcagcattct ttctgcttca
     3061 aatatatgat cagctgtaga aggtggaacc aaggaataag atgctgtcac ttttggttct
     3121 cgaggttgcc ctactctaca tctagctgat agatatgctg aaagaaacac agcgacatta
     3181 tgttttgtgc gttccatatc aacatctttc gcgcttggtg tctcatattt ctttacatcg
     3241 aaaagtacca tcctctgatt cccccaatta tgatataata cttatgtcgg aaatattatt
     3301 catagtcgga ggaatccgac tttttttatt ttgtacgtga aatgagttct cctgatttat
     3361 acgcctcagc aaattcaacc aaagctactg ccttcattct gtctttttct ctatcccacg
     3421 ctctaaactt cggtatcatt cgctgtcctc ctcgtatttt tcaatcaatt ccattacttt
     3481 tttcactatt tcaaactcaa ccgcttttga ttcttcgaaa tcatgaacaa tttctggaaa
     3541 cagtacatca tcaactaccc acaaaataat ctggcgcttt ccaccaaaat ttatgattag
     3601 atggtccgat tccacagata gtgttgctcc tgattcgata tcatataaat ccatgctgaa
     3661 ttgaacgagt ttttttatca tttgctgtcc tcctcaaata ctcttctaat atttctttat
     3721 acttctctac aaatttgaaa cgatcttgat gaagtttctt gctccaattt gtttgccgat
     3781 ccagctcacg catctgatcg aacccttttt gaatttcgtt gtaataaaat tcaatgtttg
     3841 ctgctgcttt ccaatgcctg ctacttcgca ctcctgctcc tgtttcagcc atttctaact
     3901 taactaattc agctcgttct tttgattttt tgtctttctg aatcttcatc atgattttct
     3961 tgaggatgat gtcactgtat tgtgtaatga gatccattat ctctcctcca catacctaaa
     4021 ctgtcgtcct tttgaatcaa tccataagct cctagctcta tcccagataa tgtttttgct
     4081 taatccagta atttcagata actgttcagc tgtacctgtt actagaattc gatcaccatg
     4141 ccagattgca atttttctcg gcgttttccg tttaggcttt tcagtccaca ttgatttacc
     4201 gagcttttgg acttctgcaa ctatttcttt gtcttcctgc caagattctg acttggttaa
     4261 ttcagcaatt cgtttcattg tcgctttctt atccacgctc attcctccaa tctacgaatt
     4321 tcccttctta agttctctat gtgcaaatcg attgcctttc tcgccgtttc attgaccatc
     4381 actgcctttg ttcgttctag atcgtcaatt tcacgctgaa tgtttcgaat acgcatttga
     4441 atcacttctt ctgttgtcat gatggaccac ctcgttaaaa acgctcttcc ttgaacgtat
     4501 tccgatattt tttagctaat atcaacggcg cttgatattg atgacagaac aactttgcct
     4561 tgatcttaaa gtcttttgtc tgcattcctt tgacatctac gactttgaca agtttgccgt
     4621 ttttataaaa tgtgaagtcg ggaatatact cgatcttgcg atacttcttt ccgtctagtt
     4681 caaatttcgg catcagctca aatctttcct gaagttttac tttccagcca tttgcttcag
     4741 cttgccacaa ggctagatcg taatactctg cttctgcgat agaatcaaac ttgatacctc
     4801 gatgaacagt ttttttatta cggtatttat tcatgcgata ctacctttca ctggttttat
     4861 gcgcttgtct gctgtttgtt ggaatttcag cgcataacct tctgaattct taaatatcct
     4921 agaaacaatt ctttcaccgt aggcttctct tagttcagga ccagataagt ttgttgtgat
     4981 gatcgttgcc ttgttctgtc tggcttctaa gagcgtgttt aacgtgttgt ttgtaaactg
     5041 cctactattt gataccccac tacctaatta agctccaata tcgtcaaaaa ccaccaaatc
     5101 agttgtttta atatcggcta taagcgatcc ttcaatttct tttctcagtt cagtattgtt
     5161 ataagaaaac tttatttgct ctaataattc ttgatagctt ataaaaagta ttttcttgtc
     5221 ataatttgag cgctcaagta tttcccaagc tgtggccatt gataagtggc tttttccgct
     5281 tcctgatttc cctgatagaa tgaaatgtgc aggatggttc agcagaacct cattcgtata
     5341 gctttttgct ttttctaaag cgatttttgt ttcttgatcc actacgtgat aattttccat
     5401 tttgcattta aacaaagttt tatctgttaa taccgaacca ttttgaaaaa aactcaacgc
     5461 tcgtgctttt aagctatcgt tatatatccg ttcggtctgt atatcctctt tcacacgtaa
     5521 cgctttataa ccacaactca tgcatgttgg tttacaacgt tctgaaccat ccttattttt
     5581 agctcgccaa ctatacaaag gttcgctaca ttctggacat tttccgcttt gcactaatac
     5641 tcttcttatt agtttctcca tagcatttgc taggctttcc atgtgatgca tctccttttt
     5701 aaattggcaa gtcgtcatat tcactaggat tgctgtactg tagtttttga ctttgctttt
     5761 tatgattctt cttgtctgct ttgatttcga atttgagctt ctcaaatttt tctctcaatt
     5821 tcttagcact tctaatattt ccaaaccaaa attcatttgt aggtagccaa ttgatcacat
     5881 actcaatcgc ttctatagat gctttatctc tttcttccat caacctgatt gtgtctgccc
     5941 atttttcgat atctactttg ttcatttctt ttggaaaatc ttcagttaaa ttactttgca
     6001 actttttagc aaggcgtaag tgttcgtcag aatacttacc tttcttttct tctttatcta
     6061 tatctttatc ttcttctata tctttatctg taccgtcacg tgacgtcacg ctaacgtcat
     6121 tttccaattt gagacgttcc tgtctctttc tttccctgta tttacggttt ctttcagcat
     6181 tttttagcct tactttatcc ataccctcga tattttgatg tttttcccaa ttactgatgg
     6241 caattagtcc atcactgctt agatcaatca tgttgaaatt tgccaatgta gttagcgcta
     6301 agcgaaccgt atttacgttt ttgccaaaca atgtagcaag catttcttcg gtataaggca
     6361 tgttcctctg gatatatatc agaccatcgt cgttagtctt tcctgctaaa actagtaatc
     6421 gaatccatat aacgatgatg gcatccgact caggaacagc ttggattaat cgtatttttt
     6481 catcgtcaaa catagtagtt ttaagtttga tccaacttat ctcagccaaa tttatcctcc
     6541 tatccttaac tttttaattg tttcctggtt taacttgatc cctttgattt gatatttatt
     6601 tttgaaattg atcacaccta ttttgtgttt ctctgtgtga tggattctgc agagtgctgc
     6661 aaatgtgtac tctgaatgat caacttcttt gcgctttcgt cttcccagcg ctttgtcaaa
     6721 gtgatcgata tcagctcctg ttttgccaca gatgcagcag actctttttg taatgcattt
     6781 gtagaagtaa tactcttgat tcgctggtaa aatctcatat ccttctttga aaggaatatg
     6841 atgttcaaag atgaaatcta ggatgatatt cgctaagata ttggcatcac tcacggttgt
     6901 attcgattcg tctttgaggc ttatttcgcg ccctgtgaca ccttcaaaac ggaagtagaa
     6961 gaattccttc cagaagtccg ttggcatgcc tgtatcgatg aaaatatcgc ctatgagcgc
     7021 atagatgaag tttctttgct gtactgtgaa ccgtcgtgga tcaataaagc gaatttcaat
     7081 gactcgatcg ccatcatatc cgtcatacat cgttttcagc cgttcgatat ttacttcttc
     7141 gttgattgtt gcaccaatgt cgttaccttt aaatttcttt agtaccgccg agtatgaatc
     7201 tattagtggt ttaaacactc atatcacttc tcttttgttt cttctctgta ctgatcttca
     7261 agccaattaa cgcctcgttt tagaatgccc aagtctctct tggtccattt actgtcatca
     7321 gcggttatgg aagctgcatc agtcaatgca acaattgctt catcaattga tttttcgtac
     7381 ttgttagcaa ccagttgtaa attcgatttg ccccaatcag gatcggccaa atattgatct
     7441 agagtggaaa gttcattttc catatgttaa aatctcctta gttatgattt tttgagtgac
     7501 tcattgcttt ggtcggcgga gtcacttttt tatttgttgc catgcttttt tcttttcgat
     7561 atgttgcttg cttaaaataa tagaacggct atttgcccac caattatcag caatcacttt
     7621 accgattttt agcgcttctt ctcgtgccat agttgctcct ttcttttgag tcaagcagat
     7681 tgattaaaac catcaatgct gcgaacaagc ttcctccgat aatactttgg tgcgctacaa
     7741 tcactaataa ccccaaaatg aatcctataa aaagtgtgtc tgtcttcttc ataatctaat
     7801 ctccctattt tttatttcta gcattctcaa atcctcaagt tcagaagcaa ttagttcagc
     7861 ttgtctatct gatagctcat cggattttct aagcgctgca cgatcatctt gtaattgttt
     7921 tctacgctgt ttaatcagac ctagaatttg atgttcttgt tgcaatgtgt aggacataaa
     7981 atcattctcc tttgccttta gaactcaaag ttttctttca aaaatctttg gagttccgat
     8041 cgttcaattc tgatgtctaa cttgctccac tgctgtgttt ttaagcctag gcttatccaa
     8101 tgtgttaatt tgtcatcacc aatgcctaaa acttttttta cctctgattt gtttggatat
     8161 ggaggaagct ctactgattt gttcataagg tgtaatcgtt cgtccaatga attaagaaca
     8221 gcattcgtaa tctgtgtagt taattcggaa actactaaat tatctggaat tgttatttgc
     8281 atgttatttc tccttctcta actctgctag tgctgcctca attggcttga tttgtttatc
     8341 tggttttcta cgcccattca taatgtccga catatatggt ctagaaattc cgatagtatc
     8401 agctaaccag ttctgagact ttccgtattt tgctaattga attctgactt tcaaaataaa
     8461 ttcttgtgac atcgtcattc ctcctctaat aaatcgattt ctggaatata tccttctttt
     8521 ttcagcgact cataaatgaa cagacgccct ttttgagtcc attttgtatt catcacaact
     8581 tttgttccgc catcagattt cggaatctca gttgtatgag attttgtgta accctgtctc
     8641 atgtgttttt tgcataataa ccattggttg cctacttttt tctgaatacc tagcttatga
     8701 agtagtttgt tcatctgttg tggagacatc ccataatcag ccgcgatttg actaatagtt
     8761 actgaatctg tagaagataa gatactatcc aaatacgaaa ttttgggttc gtattctgca
     8821 attttttgtt ctgcgattag tcttccagta cgttcttctt tcaacttagt tgctaattgg
     8881 atgattgtat ctggattaag caaagcttct tctacttttt ctggagttag ataacctcca
     8941 tgttttctaa ttgctggcaa cacttcgcta gtcacccaac gtttaaattt cttagcattt
     9001 ggttgtttgc ttcctaaaat taaggagtac aaacctgatt cgttaataat agttaaccct
     9061 ctaggcgatt caaaagtacc gttttggtag ttttgccgat cttctacatc gacgtgtctg
     9121 ttaatatctc tactaccatt ttggtaccct agaactttag ccacatcttt tccaacaaaa
     9181 aatggttcat catgtattgt tactgttctt acttcatgtt gttcaaagtt aaaaatttgc
     9241 ggtgtgttca tttttgctca ttcctttctt tgatataatt ttgaatagaa agcgaggtga
     9301 aaatagtatg gaagaattta atatggatgt cgacgcctta ttcaaacaaa ccgtattcaa
     9361 gactgttaat aaagaattca aaatagactt ccaaaatgac gaatcttttc caacagaaat
     9421 tgaattattc gaagaacttt ctcagagcgt ttctgagtct ttttctcgtc agttgcaaaa
     9481 gaattttttt gatgctcttg tggatgaatt tcatcaacaa cagcactaaa cttttggtct
     9541 ttaaaattta aagttattaa agctcctttt gcggaaggag tttttttatt ttgtttcatg
     9601 atgtttcctc cttttcttta aatatgtaag ctaataaaat tagctaattt ttattgacat
     9661 ttctatccaa ctgtcttata tttatataca gttaaataag cctagaaaaa ccctaaaaat
     9721 taacattctt agtttgccga cctcgaattc gtttatttta ttaggtgtat ttcttgttgc
     9781 ttgttagctt attaaattag cttacgagtt aaatataaga caattggata gatttgtcaa
     9841 ctacctttct atctttttgt ataaattatt atcgtacgct ctaggaaggt tgatttgaca
     9901 tgcttttcga aagggttaaa gagctctgta aaaaaagagg tatttctatt tctgaattag
     9961 aaaatagaat aggatttgga aaaaactcta tttataaatg gaagacacaa agtcctaaaa
    10021 tagaaacttt gcaaaaagtt gctgactact tccatgtatc aacagattat ttgctaggac
    10081 gcacagatga tcctaacgcg ggagttgcac cagaggaaag aaaactaacc gtggaagaag
    10141 ctttagcatc tgttatgagt agcgacggaa aaccgctcac cgataatgat agggaaatac
    10201 tatcagctat gattgaagca tatttagaga aaaaagataa ctaaataagt aggtgagtca
    10261 tttggacagt caaattgaaa tgataattaa tgaactcggc gttaaggtag aagagcgtga
    10321 aaaccttgat gccgatggcc attatgttgc ttgtatgaat accatagtaa taaaagctaa
    10381 tttatctaag tatagaagac aaagaacctt attacatgaa ttaggacacg cttctaaaca
    10441 tcatgataat tattttttat ataacttagc attctctctc cattcaaaaa tggaatatga
    10501 ggctgatcgc ttcatgattg aaaaattatt agatagatat attgcaaagt ctgaattaga
    10561 accacacaat atcaattaca tgaaatttat agaagataac aatttaagcg ttcgcttcga
    10621 accacttgtg aaagaattat taaaagctcg catctattgt tatgcagctc tctaaaattt
    10681 tttaagcaaa aaagaacata cgttcaaaaa tagaaaggtg aacaaaaatg atatatacag
    10741 aattcaaaga atggttagaa aaaagcacaa ccggatacga aacatttatc atcaaagcta
    10801 ctaattatca aattgaaaaa aacaaaaata gaccccaaaa aaaacgctgg gatgataaga
    10861 aaatagataa agctgtatta gaaatgtgga aacaagtcgt gactaacttg tatcaaacaa
    10921 ttcgtaaaga aaaaggagtt ccattaatta acgggaagga aatatggctt gaatttatag
    10981 aggaacaagg actgatcgaa tttttcaatg atagcatggc agaattagaa tttgaatagg
    11041 ggtaatattg atggcaatga taaaacaata taaaaagaaa aatggcgaaa aagcatggta
    11101 ctttaaaact tatctcggta ttgatccgct aactggaaag aaaaaatata ctactaaacg
    11161 aggatttaga acacaaaaag aagcaaaaac agcactttct aggttagaac tagaattaca
    11221 aaaaacagga atgcccacaa gtacaaatac tactttcaaa gaagcagcag aattatggct
    11281 agaaagctac aaaaaaactg taaaagaaag ttcatattca aggactaaaa taatctttaa
    11341 taaacatata tatcccaaat ttggaaatat taagctttct aaaattaata cggcatattg
    11401 tcaaaaggta gtaaatgatt ggagtgaaaa aggaacttca aagcagtacc ctcttttcat
    11461 aaactatatg aataaagttt ttaagtatgc tataaatatt ggtttaacat ctgataatcc
    11521 aacattaaat ttacttattc caaagccaca aattaaaaca gaaaagaaat taaaattata
    11581 tacaaaagaa cagttggaat tatttctaaa tgaagtatct caagaacaga atccatattt
    11641 taaaaacaga gactatac
//
LOCUS       DACEZN010000081        11549 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEZN010000081 DACEZN010000000
VERSION     DACEZN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.081"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1031)
                     /locus_tag="IXH60_001743"
     CDS             complement(<1..1031)
                     /locus_tag="IXH60_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1990660.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IXH60_001744"
     CDS             complement(1113..1802)
                     /locus_tag="IXH60_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1990661.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IXH60_001745"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IXH60_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1990662.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IXH60_001746"
     CDS             complement(2329..2832)
                     /locus_tag="IXH60_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1990663.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IXH60_001747"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IXH60_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1990664.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IXH60_001748"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IXH60_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1990665.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IXH60_001749"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IXH60_001749"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1990666.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IXH60_001750"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IXH60_001750"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1990667.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IXH60_001751"
     CDS             complement(6444..7463)
                     /locus_tag="IXH60_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1990668.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IXH60_001752"
     CDS             complement(7636..8523)
                     /locus_tag="IXH60_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1990669.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IXH60_001753"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IXH60_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1990670.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IXH60_001754"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IXH60_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1990671.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IXH60_001755"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IXH60_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1990672.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IXH60_001756"
     CDS             9754..10398
                     /locus_tag="IXH60_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1990673.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IXH60_001757"
     CDS             complement(10454..10957)
                     /locus_tag="IXH60_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1990674.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3376 a         2387 c         2107 g         3679 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agcttatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggagga
//
LOCUS       DACEZN010000082        11528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEZN010000082 DACEZN010000000
VERSION     DACEZN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.082"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1211
                     /locus_tag="IXH60_001758"
     CDS             <1..1211
                     /locus_tag="IXH60_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin-binding domain-containing protein"
                     /protein_id="HAQ1990675.1"
                     /translation="KQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQEL
                     TKRLLERPNEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYP
                     TLSQLLDSYYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYR
                     RNGELLTTFLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKN
                     AVKLIGEQIQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRK
                     KPSQPDQYFSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPS
                     DETITEAAELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEE
                     EKITAMKQNR"
     gene            complement(1369..2271)
                     /locus_tag="IXH60_001759"
     CDS             complement(1369..2271)
                     /locus_tag="IXH60_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1990676.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2268..2972)
                     /locus_tag="IXH60_001760"
     CDS             complement(2268..2972)
                     /locus_tag="IXH60_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1990677.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3003..3644)
                     /locus_tag="IXH60_001761"
     CDS             complement(3003..3644)
                     /locus_tag="IXH60_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1990678.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(3682..3996)
                     /locus_tag="IXH60_001762"
     CDS             complement(3682..3996)
                     /locus_tag="IXH60_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990679.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(3984..4613)
                     /locus_tag="IXH60_001763"
     CDS             complement(3984..4613)
                     /locus_tag="IXH60_001763"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1990680.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(4620..5336)
                     /gene="pyrF"
                     /locus_tag="IXH60_001764"
     CDS             complement(4620..5336)
                     /gene="pyrF"
                     /locus_tag="IXH60_001764"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1990681.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5333..6265)
                     /locus_tag="IXH60_001765"
     CDS             complement(5333..6265)
                     /locus_tag="IXH60_001765"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1990682.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6262..7038)
                     /locus_tag="IXH60_001766"
     CDS             complement(6262..7038)
                     /locus_tag="IXH60_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1990683.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7092..10277)
                     /gene="carB"
                     /locus_tag="IXH60_001767"
     CDS             complement(7092..10277)
                     /gene="carB"
                     /locus_tag="IXH60_001767"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1990684.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10282..11358)
                     /gene="carA"
                     /locus_tag="IXH60_001768"
     CDS             complement(10282..11358)
                     /gene="carA"
                     /locus_tag="IXH60_001768"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1990685.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(11363..>11528)
                     /locus_tag="IXH60_001769"
     CDS             complement(11363..>11528)
                     /locus_tag="IXH60_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1990686.1"
                     /translation="APADVAVFDITQTCTIDKEDFLSKGENTPFIGWKVKGETQMTFV
                     NGKLVWQKGE"
BASE COUNT         3231 a         2604 c         2038 g         3655 t
ORIGIN      
        1 caaaacaaga ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt
       61 cccaaactga actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata
      121 tcgctcaaga gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg
      181 acgaattttt ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata
      241 aagatttttt cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag
      301 cttatccaac actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc
      361 gggcaaaaca acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata
      421 aaaataaatt gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc
      481 gtagaaatgg cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag
      541 ttgttttacc taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat
      601 tgacaccaaa tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg
      661 ttaagctgat tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg
      721 tcttatctca actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga
      781 cttcagaagg atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc
      841 agcctgatca atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc
      901 aaaatgacca gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa
      961 atatacccgg ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag
     1021 aagctgccga gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg
     1081 atcttgtaca ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt
     1141 atgaaaacca aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac
     1201 aaaacaggta atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa
     1261 ttagttataa attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct
     1321 cttttttgat caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg
     1381 gtccagtaac cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt
     1441 cgccgaataa atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa
     1501 gtgctcgcga atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag
     1561 aacgatattc atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat
     1621 ctcgataagg tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg
     1681 aggataagat aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg
     1741 ccttaaaaaa atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg
     1801 taaatatctt ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact
     1861 gtgctgctct ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg
     1921 tttgatctcg tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata
     1981 atgctgggca gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa
     2041 tgtatcaagc tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt
     2101 taaatatgcc cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc
     2161 tttgatacca gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa
     2221 actaccattg tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac
     2281 tcctcgaaat aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga
     2341 gcatactgac catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt
     2401 acaaaccatt ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa
     2461 tggctttttt gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag
     2521 ttccaacttt ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac
     2581 atgacaccca caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata
     2641 tcttcctgtt ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa
     2701 taatagcgtt ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc
     2761 gtgtttttga attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct
     2821 ccttctattt tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc
     2881 gcacctctcg caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa
     2941 tagccccatt cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat
     3001 ttttattttt cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca
     3061 taaaattcct ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc
     3121 cgatgttcaa agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt
     3181 tccgggttat ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa
     3241 atggctaata gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa
     3301 acttcttgga actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca
     3361 actgtttgca gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca
     3421 ccaataaaac gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg
     3481 atcggttgtc cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga
     3541 tacgaatgtg ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc
     3601 gtctctaaat tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt
     3661 tgagatttct ttttccgaat attatatcat taaataactg atattcgcaa catttccgct
     3721 ctttaccatt tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga
     3781 aaaaagagaa gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa
     3841 aaaaatcaaa aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct
     3901 cgcaatcagt ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat
     3961 ttctctatct tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct
     4021 tttagcagag ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat
     4081 acagaataat tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct
     4141 tttggcaact catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt
     4201 gctgcttcag ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc
     4261 atcttctgac cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc
     4321 ttacttcgaa tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg
     4381 attcctgctg ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg
     4441 cttgctaatc cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta
     4501 tcacagtaaa ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa
     4561 aatactgctt caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt
     4621 tagctccatt ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt
     4681 ggtcgaccta taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact
     4741 cttgtctgat ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat
     4801 tcgtctcctg ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct
     4861 aatcctgctt ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg
     4921 attagttgat cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga
     4981 agtagcggac gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc
     5041 atcatacgaa taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat
     5101 ccccgcattg ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca
     5161 ttatgaccag cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc
     5221 attccgattt tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc
     5281 tcttgcctag ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt
     5341 cacgttcctc tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca
     5401 ttcgcacagg gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg
     5461 tccctatagc tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct
     5521 gcacgccgcc catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac
     5581 gaatagcgac aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg
     5641 gttttcttgt cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat
     5701 ctgcccctcc agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca
     5761 atttgacata gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa
     5821 catcagaatc tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca
     5881 gctcgattgc tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt
     5941 cttcacaagc accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg
     6001 gaagaagttt atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca
     6061 tgccagatgg tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg
     6121 ttgccttgac catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc
     6181 caaaaccgaa acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt
     6241 tgaccgctaa aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc
     6301 gtcacaaact ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc
     6361 atagcaagcc cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg
     6421 cgaagaaaac agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc
     6481 tgtaggttcc gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt
     6541 tccatcgtca gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac
     6601 gacttcatgt gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg
     6661 ttttgataac tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc
     6721 tcctgtatga agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg
     6781 gtctccggca gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat
     6841 acggcaagta tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa
     6901 gtctgcacgc ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag
     6961 atcacctttc aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc
     7021 catcatttct tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca
     7081 tgtgctcgcc gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc
     7141 gcatctgctg tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg
     7201 atgatgaaac cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact
     7261 tgtgttttac catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt
     7321 ttcgctactg tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt
     7381 cctatcagac tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt
     7441 gtttcgtccg caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct
     7501 tcgaatgcct tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc
     7561 gatttcattt caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca
     7621 ggtgctttaa catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc
     7681 tttagtttct ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc
     7741 ttacttaaga atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca
     7801 ttttcatgga tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag
     7861 cggatcgtgt aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac
     7921 acagccattg aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg
     7981 atgagtactg tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa
     8041 cggtccacta gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct
     8101 aaatctcgct ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga
     8161 acaagaactg gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca
     8221 gtatccccta aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga
     8281 ttttccgcac gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc
     8341 aaaggttcgg ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc
     8401 tgttctagct cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta
     8461 tccgaaatag aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg
     8521 tatcctgcct gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct
     8581 tgtccgatac ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa
     8641 ctttcatttt catattcata ggaactataa aaataaggtg tttttgattc gaattccgct
     8701 gcacaagtgt caaccatctt atatacagga atcaaatcat tttcttttct catctggcga
     8761 acggcttctt cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt
     8821 ttcgctgttt tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct
     8881 acgatatgca acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct
     8941 tctatggtgt accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct
     9001 cgaaccactt tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata
     9061 tggtgtgtac cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga
     9121 ccgatagcca tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt
     9181 tcaaacttat caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca
     9241 aattctgcaa aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc
     9301 gctattttag cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat
     9361 gaacgagaaa cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga
     9421 gcgagttgga cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca
     9481 tcacgaagca tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa
     9541 tcacctgtat ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta
     9601 tcagccgaat ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca
     9661 atcaaacatt gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct
     9721 tcttcatttt cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg
     9781 acaggatagc cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca
     9841 gactcgggga ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct
     9901 gcttgatcga tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata
     9961 cctgatgctg ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg
    10021 agagcatctg gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg
    10081 atatatactt gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact
    10141 aaaaccactt catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca
    10201 aattctgcgg cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata
    10261 tctgtacgtt tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc
    10321 atcaaataaa tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact
    10381 gaaagcggga taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg
    10441 agttacgatg agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg
    10501 agaagtaaaa tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg
    10561 tccgaatttc attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag
    10621 acaaatacca aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg
    10681 cacatctttt ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa
    10741 aatcgtctcg gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa
    10801 ttctcgtaaa atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc
    10861 tggacttgga taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt
    10921 tgctttcagc tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt
    10981 catcgttccg acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc
    11041 aggaattcct ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc
    11101 tacacgtgca tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc
    11161 atctctattt accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt
    11221 aaaactttga tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc
    11281 tccgaagaca ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa
    11341 tataagcaaa cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa
    11401 aagtcatttg tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg
    11461 ataaaaagtc ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag
    11521 cgggggca
//
LOCUS       DACEZN010000083        11498 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEZN010000083 DACEZN010000000
VERSION     DACEZN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.083"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            174..875
                     /locus_tag="IXH60_001770"
     CDS             174..875
                     /locus_tag="IXH60_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1990687.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1484..2500)
                     /gene="tsaD"
                     /locus_tag="IXH60_001771"
     CDS             complement(1484..2500)
                     /gene="tsaD"
                     /locus_tag="IXH60_001771"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1990688.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2497..2961)
                     /gene="rimI"
                     /locus_tag="IXH60_001772"
     CDS             complement(2497..2961)
                     /gene="rimI"
                     /locus_tag="IXH60_001772"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1990689.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2968..3510)
                     /gene="rimI"
                     /locus_tag="IXH60_001773"
     CDS             complement(2968..3510)
                     /gene="rimI"
                     /locus_tag="IXH60_001773"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1990690.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3494..4318)
                     /gene="tsaB"
                     /locus_tag="IXH60_001774"
     CDS             complement(3494..4318)
                     /gene="tsaB"
                     /locus_tag="IXH60_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1990691.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4407..5387)
                     /locus_tag="IXH60_001775"
     CDS             complement(4407..5387)
                     /locus_tag="IXH60_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1990692.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5411..6895)
                     /gene="galT"
                     /locus_tag="IXH60_001776"
     CDS             complement(5411..6895)
                     /gene="galT"
                     /locus_tag="IXH60_001776"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1990693.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6907..7896)
                     /gene="galE"
                     /locus_tag="IXH60_001777"
     CDS             complement(6907..7896)
                     /gene="galE"
                     /locus_tag="IXH60_001777"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1990694.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8144..8311
                     /locus_tag="IXH60_001778"
     CDS             8144..8311
                     /locus_tag="IXH60_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1990695.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8373..10184)
                     /locus_tag="IXH60_001779"
     CDS             complement(8373..10184)
                     /locus_tag="IXH60_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1990696.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10181..10546)
                     /locus_tag="IXH60_001780"
     CDS             complement(10181..10546)
                     /locus_tag="IXH60_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1990697.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10709..11104)
                     /locus_tag="IXH60_001781"
     CDS             complement(10709..11104)
                     /locus_tag="IXH60_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1990698.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11122..>11498)
                     /locus_tag="IXH60_001782"
     CDS             complement(11122..>11498)
                     /locus_tag="IXH60_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1990699.1"
                     /translation="GRFLAEEYAGDSKLKAMLDNGEEVTSKDIFEMAQEDDPFALMVV
                     DRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFLRSRVEKYFKEFTFPQVRESTKI
                     KLAELGNEAGVIGAASLALKFA"
BASE COUNT         3281 a         2438 c         1988 g         3791 t
ORIGIN      
        1 aaatcattcg cttcaaaaca aattattcaa taccattgta tattttttac ataataacaa
       61 attatatttg taaagtatta aaacttgttt ctaatgttgg tatgttaaaa ctttacaaat
      121 tctttaaaaa aagtgacaat aaatatagtg aacaataagg agaggacttt ttaatgcgtg
      181 ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt gaacatttct
      241 cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat aaaaaaggac
      301 agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc aaaggactcg
      361 ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat gtgaatacaa
      421 ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat acagctcaag
      481 cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa
      541 aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt gaaatacatg
      601 aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa caaaatttat
      661 tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc attccttatc
      721 cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact gctggacaag
      781 taatcaaaca actacgtgat tccgggctgt tagattatga caaaaagcgt tttactttct
      841 gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa gcagtgaccc
      901 tgataaatat cagggtcact gcttattttc ctccctaaaa cttctgaaag atccggcagt
      961 tttagatcac tggacaagac tcaaagattt tctatgtgtg tttcagttat gtaatattca
     1021 ctgtttgcaa aaaatactgt tagattaact agatgaagcg tcttgtcctc atccgatcta
     1081 gattattcga tattcctcga caataaacga ttactcagtg agggcaatca gaggaacgga
     1141 taatgataaa taagctgtaa aaaatagcaa aataacaata atgattaaaa gtatgtaaaa
     1201 aatagggctt tgacaaaaac gtgtcacagc cctatttcga ttaaacggtg tgtacctgtc
     1261 aactcttcgg tcacaagtca taatgttcat aaacatgagg agctgttttt tgaaattgtt
     1321 ccttgtgaat cagagttaaa cgacaggctt acgaactctt gattaaacgg cgttcaaaag
     1381 cttgcatacg cggtaataag aacagccagg tcaaaaacat gagaagccgt ttttcctggg
     1441 ctgttcttat tacttgatgc agggcagctt tttcacaacc tctttattca gataaaacca
     1501 agcttggatc agcattcaac tgatagctag caaaatttct tttttgcatt tccacatgtg
     1561 ctgctgctcc aatcattgca gcattatctc cacaaagtcg aagaggggga atcactagtt
     1621 ctacctcagg gagtttagtt gccaaagcat cctgcaatcc ttcacgcaac ccttgattgg
     1681 ccgctacacc tccagctata atcagttgct tgactggata attttgacaa gctcgaagtg
     1741 ttttagtaat caatacatcg atcacacttg cttgaaagct agctgctaaa tcatttttgt
     1801 ctagatcttc tccacgctgt tgtgcattat gcaccaaatt gataaatgca ctttttaatc
     1861 cgctaaaact gaaatcataa ttatcttcat ggatcatcgc tcgcggaaaa tggtaattat
     1921 cttttccctg atgagctagc tgatcgattt cttttccgct tgggtaagaa agacctaata
     1981 ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc ccaatgattt
     2041 catatgatcc atcttcttgc atatagacta attctgtatg tcctccgcta acaagaagtg
     2101 ccatcaatgg aaactggaat ggtttaacca gacgggctgc atagatatgc cctgccatat
     2161 ggtttacagg aataagcggc aattggtgtg cccaagcaaa agccttagct gcagaaatac
     2221 caatcagcaa cgagccgaca agtcctggtc cataagtgac agcgactgcc gataaatctt
     2281 ctgccgacac accagcttct accagtgcat cttccaagca taaggtgatt tgttctacgt
     2341 gatggcggct agctacttca ggtactacgc caccaaaacg tttatgactg tttatttgag
     2401 aagcaacgat gttagataag atctctgttc catttctaac gaccgctaca ctcgtttcat
     2461 cacaacttga ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct ccgtttttaa
     2521 ctttctgctc ataatgatcg catcttccgt cggattgcga taatatgatt tccgtttacc
     2581 taatatacga aacttttctg attcataaag ttttcttgct gcttcattag atagacgtac
     2641 ttctaaaaaa acatgcagcg aatcttttat ttgttccgta atcagtaatt gtaacaaatg
     2701 ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca atatttgtga tctcgacttc
     2761 atctattact ttcgagtatc caataaagcc acagatctgg ttatcttcag ctaaaagcag
     2821 ataatcagag tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc aaggtgaacc
     2881 atgtggataa gcattttcgc taatcatcca tacgcattct gccaagtacg cttcatcaaa
     2941 atcttttttt ctgcaaatca actaatctca ctcattttct aaaagtttta tcatatccaa
     3001 cgcatcttct tttgtttcag tgtaatatct ttttttgatc gttgttgctt gaaaccccaa
     3061 tttccgataa agtctttgtg catcttgatt actcatacgc acttctaatg acatcgtctc
     3121 acagcgattc attatagcaa agttctcgat ttcgtcaata agcagcgaac cgattcgttt
     3181 acgctgatat gccggtcgaa cggcaatatt cgtgatatgg caatctaaac cgaacgctct
     3241 tgaaccgata aaaccaatca ttcttccatt atcaaagata cctagatata gatgaggaag
     3301 cggtgacttc aattctgata taaaggcact ttttgtccat ggcaattcgc cattatagac
     3361 ttctcgttcg acatccagca gctctttgat atcttctgta gtcaattctc ttagtacgta
     3421 acgacgatcg atttgttcga ctgttttttc tgtataaatc attttatctt gaaaaaagct
     3481 tctggcatac gctctaaatt ttttcaacat aactttcgtc tccaggtgtg tgcgtctcca
     3541 accatttttc ttctgcttct actcgtttta aatagcgagg aagaaaagct tgtttctctg
     3601 caactggttc aactaaagca cctaattgag ccacagttac accacttgga atatcccatg
     3661 ctggaactgt attaatcatc gcactaggca atatttcctt tatttcagag aagaatttgt
     3721 gacaatcact cccgacgaaa taaatagctt cattcagctt ttgtagttgt gtgaacaaat
     3781 cagaagcaga aatatgtaca tcttcaagta cagtttctaa tctattttct ttccagcggt
     3841 atgccccagc gtatacattt tttcttcgag catcaaacat gggcacaata atattatcca
     3901 caccgatgca attagccgcg atggttttca gactagatac tcctactagc tctttatcta
     3961 atgtataagc aatcgttttt gctgtcgtta cacctatacg aagccctgta taagaaccgg
     4021 gaccatcaga aaccgcgatt cggtcaatgt cttttggact tatttgaaga tctgcaaaca
     4081 attggtcgat tgcaggcatc agtgttacac tgtggttgcg tttgatattt gtttgtattt
     4141 gtccaagaat ttgttcgtct tctaccacac cgatcgccaa agtttggttt gcagtatcta
     4201 ttcctaatgt aatcatatat ttgatccttt ctgctatgac attctcgcct tccattgtag
     4261 catacttttt tattctgagg atgaaaatac caaaggcaaa actagtacaa taatacatga
     4321 ttatctgctg gcgggcaaat tttgcccctt cctgaaacac aaaaaagctg cggtatcact
     4381 ccattttccc acagcttttc catcttttat ttcgttgatt ctcgaataat caactctgtt
     4441 tccacaataa tctttcttga aaccggtgct ttggaattac ttaattgaag aatcgtctcc
     4501 actgctagct ctcccatcca ttcggtataa acttttacag tagaaagcga gggcgttaca
     4561 tatttcgcta cactgacatc attgaagccc atcacagaaa tatcttctgg taccactaaa
     4621 cctttttctt gaatcgcttt cagtgcgcca atcgctaatg catcattagc tgcaaaaagc
     4681 gcatcaggaa catgcggatt cgtttttaaa tactgcttca ctgcttgata tcctgactcg
     4741 acagaaaaat ctgcttcgat aattggacct tcgtcgtagc ctttttctgc caaaacttgt
     4801 tgaaatgcca taattctggg atcttttagc cgctgatggt ttttcttcgt atattctgtt
     4861 ccagcaagca tagcgatatg ttgcgcttta tgggagacaa agtgttcaat gactttttga
     4921 acacttgact taaagtcgac aactagagag tcgattcctt gagaagtgcc gtcagaatct
     4981 accaaaagca aggcattttg ggatcctttc atttctaata actgagcttc gtcgaatttt
     5041 cctagtgcga tcgttccatc tgccggaaga tcactaatgg aatcccacgt ttctttcaat
     5101 aaagtaacgt ttgcttcttc tgcctttttt tcgattccta ggcgaataga taaataataa
     5161 agatcttcaa gctcttccgt atcgtcgtac cactggatca ggcgaagcgt ttgcttagta
     5221 tttgctgcat ttttacgatg tttcgtataa tttaagcgtt ctgctgcttc aaaaattttt
     5281 tgctttgtgg ctaagccaac ggaaagatca ggatcatagt tgagaactct tgaaacagta
     5341 gcaggagaaa ctcctgccaa ctgcgcaatg tctttaattg ttgccatttg tctctcctcc
     5401 ttttttacgt ttataaaatc gcagtaaatc gttcaaacgc tgcctgtcct ttttcatctc
     5461 gcttgaatac tccagcgtct tccagtacac gtgcaaaaac ctgacctaca gatttttgta
     5521 cgacatcggt gaccgtatct ttcgtcaaat caggatgttc tttttttaat tgttctgccc
     5581 acggacgatg atattcagcc atctggtttt cttctcctaa taagaaatgc tctacttcta
     5641 ctagttctgg tttcaaacga ggtggtaaaa cagcaagccc cattacttcg atcaacccaa
     5701 tgttctcctt tttgatatgt tggacatctt tatgtggatg aaaaatccca tcaggatgtt
     5761 cttctgaaac attgttgtct cgaaggacaa ggtccaactc aaagtaattg tcccttcttc
     5821 gtgcaattgg cgtaatcgta tggtgtggtg taccatctgc tgaaaaagct ctgatagata
     5881 ctgtttcatc cgaatatgct ttccattttt ccaatactga gttagctgct tcaataagca
     5941 agtttttctc tgttgattgc aaacggatca cagacatcgg ccatttcacg actcctgcga
     6001 tgacttgcgg ataatcttct agtttgataa gtttttctat cggtgctttt gccataggaa
     6061 attcatgacg tcctgcctga taatgatcat gagaaagaat cgatcctcca acaattggta
     6121 agtcagcatt cgaaccaaca aagtaatgag gcaagacttc agtaatccga agcagtctag
     6181 caaatgtttc ctttgtgatt ttcatcgggc ggtgttgttc tgataaaatg atcgaatgtt
     6241 cattgtaata agcataaggg gaatattgga atccccagct ttcaccgtct agattcattc
     6301 gaataatccg gtgattggtg cgggctggat agttcaagcg acctctatat ccttcgtttt
     6361 ccatgcaaag catgcaggta ggatagtcta cttttaccgc ctcgcgttct gctgcgattt
     6421 gttttggatc tttttctggc ttagataaat tgattgtgat ttccagttca ccatactccg
     6481 actctgccgg aaatttgatg ttttttgcga tggctcttgt tttgatgtag tcatttttct
     6541 tacttaaatt aaagaaatac tcagttgctt cctctgggct tttcgaatag tgctgtgcaa
     6601 agaatgcatt gacgactgaa ggtggcggtg tgagaaaatc cattaattgc gcttcaaaaa
     6661 tctcttgttc tgctggcaat tctgaaagaa caccattttc tcttgcctta gcgacaagct
     6721 gatccaataa gtctacagat tcttccttaa ctggtcttac ttcaatgctt tctagagcat
     6781 cttctcctac catccctaaa agacgattct gtaaatataa gcggtccatt tccatccatc
     6841 cgccagcttc gatcgcaagt gtggcaaaat cacaaatcgt ttgactgatt gacatatctc
     6901 attcccctat tcttcataac catttggatg gctgacatgc caatcccatg ctgttttgat
     6961 aatcgctttg atatctgtgt attcaggttt ccagcccaga atctctctcg ctttttcact
     7021 tgaagcgact aatcggctag gatctccagc tcttcttggt gctatttctg ctggaatttc
     7081 ttttcctgtc acttctctag ctgcttctag catttccttg actgagtatc ctttgctgct
     7141 gcctaaattg aagaagttgc tttcatttcc ttctttcaag tattccaaag ctaaaatatg
     7201 tgcagcaatc aaatcttcta cttgaacata atctctgata catgttccat ctggtgtatc
     7261 ataatcgtct ccataaacag ccaaggcttt tctttgacca agagctactt gtaaaatgat
     7321 tgggacaaga tgcgtttctg gtgtatgatc ttctccgata gatgcatctg cttttgctcc
     7381 ggctacattg aagtagcgca aagcgacata gcgcatgcca tatgcttggt cacaccactt
     7441 catcattttt tccatcatca atttactttc accatacgga tttttaggat tagctggtgt
     7501 atcttctgtg attggagact ctttcggctc tccataagtt gctgcagtag aagagaatac
     7561 gattttattc acgttgaact cgtgcatgac ttcaagtaag atttgcatac cgtatacgtt
     7621 gttgttgaaa tacatcaatg gtttttcgac agattctcct actaaagagc ttgcagcaaa
     7681 atggattact ccttcaatcg gttccttttc aaatacggaa cgtagaaatt ctttatcccg
     7741 gatgtctcct tcataaaaat gagcatctgg atgtactgct tgtttgtgac ctgtgagcaa
     7801 attatctacc acagctactt gatacccctt ttgaactagc tggtctacag cgtgcgaacc
     7861 gatataaccg gctccgccta gtactaaaat tgtcataaaa aacactcctt tgattagtaa
     7921 aattgtgttt gttgattcta tataatttaa aaaacgaagt ttacttttat ggtgaaaaca
     7981 aacaaagtaa aatttcgtaa attcatatgg taaatttata ataaaacatt tagtaaacat
     8041 tttcaaggat tttttgcaaa ggtttacaaa caaacgtaaa actagccttt ttttctcttt
     8101 tgttccataa tagatataac aaataataaa tgaggtggat tcaatgaaaa aatttgtatc
     8161 tggaatatta gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg ttgcttctgt
     8221 gaaaaaaaca gtgatcgatc caattgacga aaaagaagca atgatcgaag aaaaccgtaa
     8281 aaaagcaatg cgtaaacgcg tttctcgtta ataattattt aaaaatacgc atgaagactt
     8341 tctaaaaaac agataagtca tcatgcgtat ttttatttgt cagtcaatat aagttgtgct
     8401 attttatctg cctgtctcag attccatgga tctgtataag gtctagctac tgctgcagtt
     8461 agccaatcca caccttcaac aggtattcca tagcaataaa agtcagactg gatttcccct
     8521 gaagcatcaa tcacgcgatt cgtacctcgc tcaacatcta tagcttctga atgataactt
     8581 cctgtattat ctgaaaatga aaaagggcga acaagtttgt tttccttcaa attttttatg
     8641 acaggattac ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat caaatagcga
     8701 ctgctgattt ctttatcttg tagagaaata acatagcggt tgtgcttttg ctgaatatca
     8761 aatggcggtt cgatcaaatg aaaaataccc gcatctatca gtgctgctaa ttctttcgtc
     8821 cgaatgattg gcggaccgat cgttaaaaag ctatttagat gagtaaacca tccccatagt
     8881 atttctttgt attccttagc agaaaaaacg tgccattcaa tagcctgccg gattggatca
     8941 cgccagtctt ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt gcctttttcc
     9001 gcttctttta tctgcccttc aataaatatt cggctagctt ctgaaaaggg ctcattctga
     9061 taggcaagag gagtttctaa atacgaccaa tcccatctat aagggattag tgctggatgc
     9121 tttccgagac actcctctga agagagggag agaaaatcat gctcaaaatt tttctttgga
     9181 atcggcaagt gatattcttc gatgattttc ttgtaataaa acaactcaac gtcttttttc
     9241 agatagtcaa aaaatgtttc gccagtcaaa aatcccgact gatgccattg ctccaggttt
     9301 tcatttgtta gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt tcttcctcga
     9361 gggtaataag ggagccccct tcttgatcca ctgtatacaa tcggttcctt gcctgatgga
     9421 tgatagacca gttttccctt ttcgttctcg aagcgccctc ctcttgatac tgtaaacaat
     9481 ccgacataat caaaaaaact taagcctaga cctcttaaaa agacattttc tttaggcggt
     9541 atcttcgaaa gatctacatc tgcaggatta gaaggaagct gatacagtaa aacatttttc
     9601 tttgcgtagt ctacccacac tctttcttct tctgtctcat cattttccca gtgaccgctt
     9661 gctaaaacaa gctgatcgat agaatagctt tgctgatctg tctgtaaaag ataaccatct
     9721 tttcttttgc ttactttttt tactaaagaa taaacaaact gaatagaagc tggaaattct
     9781 tcttgcaact ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg agtagattgc
     9841 tgatcttttt ccagacgatt gatttcttct ataattagtt gcttattttt ggttaccgtc
     9901 tgttcgatat aatctgtagc aggtccttta ctccattgaa acaagttggg tccctgtgtt
     9961 accaatcctc cactggacaa tgtttcatct gtaaaaagcg tcacttgttg agaaacagaa
    10021 ttcattaata actccggtgg ctgattcaac cgccaaactc gacctccagg accattggga
    10081 tcgaaaagaa aaagttcaag agaatgtctg tcctgattat tatggagaat cctttctgct
    10141 acacttaatc ctctaggtcc ggcaccaatg atccctattt tcatttgcat gcctccattt
    10201 cgataaattt tttcaataaa acagcatggt tctgatgttc attttttgca ccataaacaa
    10261 aagtaatgac tggatgttct ttcataagtt gactgagttt aaaaatagct tgcttctttt
    10321 cttcttcgaa aatcaattct tgctggtatt tctttgcaaa tgaagcaaat cgttctggta
    10381 catggtcaaa agttttgcgt gcttcattac taggtgcaat ttctttcaac cacagatcta
    10441 actgcgcttt ttcttttgaa attcctcgtg gccagaatct atccacgagt attctatagc
    10501 catcctcttt ttcagcatca aggtaaacac gttttaattt gatcatagtt gtttccttct
    10561 ttctaccttt tcgcttagct tatatatgta gataatagca caaaataaaa aaaatgaacg
    10621 gcacaactat ccattgcgcc gttcatgtga tcttggtcaa aatctaatga ttgattcggt
    10681 ttttaaagct gtcatacaat catctgtttt atactttttt acttttgatt tttccagacc
    10741 atccgtcata tccaccctta agtaaataaa tctcttgata acctgcttta cgtaatttat
    10801 ttgctgtacg gatgctcaaa cttttctttt gatcgtaaat atagacaggc tggtcttttc
    10861 gaatagatcc taatgaatct ttcaaaacag tgtaagggat gtttcttgca cctaaaatat
    10921 gcccagcatc aaacacatct ttttcgcgaa catcgattac ttgtgctttt ctcattcctt
    10981 ctcgaaattc ttcttctgtc aaagtcgttg ctgaacgttt ggccattact cgtagataga
    11041 cttcccaaag gatgattgca agcacaatca atagcaaaat gatattaatt acccataaaa
    11101 ccatgtaatg cctccgtttt tttaagcaaa tttcaaggct aatgaggcag caccaatgac
    11161 accagcttca ttaccaagct ctgctaattt gatttttgtt gattctctta cttgcgggaa
    11221 agtaaattct ttaaaatatt tctctacacg gctacgtaaa aattctcctg ctgccgatac
    11281 tcctccacct aacacgatac ttgacggatt taatgtattc cccaagtttc cgcaagctaa
    11341 acctaagtag aagcatacgc gatcaacaac catcaatgca aacggatcgt cttcttgagc
    11401 catttcaaag atatctttac ttgttacttc ttctccgttg tctagcattg ctttcagttt
    11461 tgaatcccct gcgtattctt ctgccaagaa acgaccta
//
LOCUS       DACEZN010000084        11390 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEZN010000084 DACEZN010000000
VERSION     DACEZN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.084"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            84..1130
                     /gene="gmd"
                     /locus_tag="IXH60_001783"
     CDS             84..1130
                     /gene="gmd"
                     /locus_tag="IXH60_001783"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ1990700.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1210..2223
                     /locus_tag="IXH60_001784"
     CDS             1210..2223
                     /locus_tag="IXH60_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990701.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2260..3204
                     /locus_tag="IXH60_001785"
     CDS             2260..3204
                     /locus_tag="IXH60_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ1990702.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3272..4585
                     /locus_tag="IXH60_001786"
     CDS             3272..4585
                     /locus_tag="IXH60_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1990703.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4669..5571
                     /locus_tag="IXH60_001787"
     CDS             4669..5571
                     /locus_tag="IXH60_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ1990704.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5576..7228
                     /locus_tag="IXH60_001788"
     CDS             5576..7228
                     /locus_tag="IXH60_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1990705.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7252..8118
                     /locus_tag="IXH60_001789"
     CDS             7252..8118
                     /locus_tag="IXH60_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1990706.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8160..8351)
                     /locus_tag="IXH60_001790"
     CDS             complement(8160..8351)
                     /locus_tag="IXH60_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990707.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8381..8920
                     /locus_tag="IXH60_001791"
     CDS             8381..8920
                     /locus_tag="IXH60_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990708.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9048..9401
                     /locus_tag="IXH60_001792"
     CDS             9048..9401
                     /locus_tag="IXH60_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990709.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9868..10344
                     /locus_tag="IXH60_001793"
                     /pseudo
     CDS             9868..10344
                     /locus_tag="IXH60_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10344..10538
                     /locus_tag="IXH60_001794"
     CDS             10344..10538
                     /locus_tag="IXH60_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990710.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10544..10829
                     /locus_tag="IXH60_001795"
                     /pseudo
     CDS             10544..10829
                     /locus_tag="IXH60_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10843..11209
                     /locus_tag="IXH60_001796"
                     /pseudo
     CDS             10843..11209
                     /locus_tag="IXH60_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(11256..>11390)
                     /locus_tag="IXH60_001797"
     CDS             complement(11256..>11390)
                     /locus_tag="IXH60_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1990711.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT         3200 a         2322 c         2885 g         2983 t
ORIGIN      
        1 attatcgtgt attcttgaaa agtattagaa attacgagcc atttatatgg tcttatcact
       61 tacataggaa aggataatgt atcatgaaaa aagcgctgat tactggtatt actggtcagg
      121 acggttccta tctggcagat ttcctgctag agaagggcta cgaagttcac ggtatcactc
      181 gccgtgcttc tatttccaat accgctcgca ttgaccattt gatgggcaag atcacccttc
      241 atgacggtga cctgtccgac tcatcttctc tgattcgtat catcagcatt gttcagcctg
      301 atgaaatcta taacctcgct gcacagagcc atgttcaggt ttcttttgat gttcctgagt
      361 actccggcga tgttgatgct ttgggcgtta tgcgtattct ggaagcttgc cgtattctgg
      421 gcctgacgaa gaagactaag atttatcagg catctacttc tgagctgtat ggcaaggttg
      481 aagaagttcc tcagcgtgaa accactccgt ttcatcctta cagcccttat gctgttgcaa
      541 agcagtatgg cttctggatc actaaggaat atcgtgaagc ttatggcatg ttcgctgtca
      601 acggtattct gttcaaccat gaatccgaac gtcgcggtga gaatttcgtc acccgtaaga
      661 tcaccctcgc tgccggccgc attgcagaag gcctgcagga ccatctggaa ctgggcaaca
      721 tggactccct gcgtgactgg ggctatgcaa aggactatgt tgagtgcatg tggatgattc
      781 tccagcatga gactcccgag gacttcgtga tcgctaccgg cgagcagcac actgttcgtg
      841 acttcaccga gaaggcattc gcagcaaatg gcatgaccat tcgttgggaa ggcactggca
      901 ttgatgaaaa gggctacgat gttgaaaccg gcaagatgct cgtttgtgta aatccacagt
      961 ggttccgtcc gactgacgtt gacaacctgt ggggcgaccc gactaaggca aagaccgttc
     1021 tcggttggaa tccgcagaag acctcttacg aggaactggt taacatcatg gcaaagcatg
     1081 accgtgaatt ggcaaagcgc gaagctgcaa tgaagaacgc agtcaagtaa gctgacattg
     1141 aataaacata tctgtttcta tctttccagt cacggcttcg gacatatcgc gcggaatctg
     1201 cccattatta tggcaacact ctgccgatgc gaagcgaaga ttacactggt ttgcggcgca
     1261 aatcatctgg attttgcaaa gcgcaatttg gagcaggaac tgcccaaaga gctgatagac
     1321 cgcgtgcgct atcgtccaat aaacacagat atcggtctga ttaaccgtga gggcacccta
     1381 ctggtggaca cgggtaagct gaccgagatc tgtgaatatt ttttggcaaa ggttccttct
     1441 ttggcaaaga cagaagccga atggctcaag cgagaagctg tcagcgcagt gctttgcgat
     1501 atgccaatct gggcaatgga tgcctgcgat atggcaggag tacctttcct gtatgtagga
     1561 aacttcactt ggacagagct gtatcgcgaa tttttacccg aacatatttg gaaagaatat
     1621 gccaagcgtt acggtaaaat tcagcacacc atgctttatg cgctaaataa cgaagaaatg
     1681 ttggaatttc tccaaaacag cgaaaaaaca ccgacttccg ttgtggcgcg tccgattcat
     1741 aacgacgaag tgcaacgcat taaatcacag catgaaaaac cgcctgtatt tgtcgcactc
     1801 ggtatgtccg cgcggttcaa acagccaatt gatgtcagtc acgcgccgta tgatttcatt
     1861 acaacgctag gtgttccgct tgtgggagaa aacgtaaccg ttatttctaa cagcaccccc
     1921 aatacacagg actatatagc ggcggcagat tacgttatta caaaggctgg atggggaact
     1981 gttgcggagt gtattctcgc acagcgtccg atggcactgt tcgcccgcga tacagttttg
     2041 gaagatagaa caactatttg taaattgcaa aaactgggtc tggcaatttc cgtcacaccg
     2101 gaaaacctcc accgcgtaga gcgggtgatt gaggacctca aacagctaga cacctcaaat
     2161 tttgatacat tttatgatgc tgcgagcgaa atcgcagaca aattgatttc tctcatgcaa
     2221 tgagagatgc tctaaattta gaaaggagca agcattatta tgatggaaaa agacgcaaaa
     2281 atctacgtag ccggtcatcg tggcatggct ggttccgcaa tcgtgcgcga gctgaaccgt
     2341 caggactaca acaatattat cacccgcacc cataaggagc ttgacctgtg ccgtcaggac
     2401 gctgttgaag catttttcgc acaggaaaag ccggattatg tatttctcgc tgctgcaaag
     2461 gttggcggta ttatcgcaaa ccagaacgct ctggcagact tcatgtatga aaacatgatt
     2521 ctggaaatga acgtcatcaa ctccgcatgg aagaatggct gtaagaagtt acagttcctc
     2581 ggttcttcct gtatctatcc gcgtatggca ccgcagccga tgccggaaag ctgtctgctg
     2641 acaagtgagc tggaaaagac caacgaggct tatgcactgg caaagatttc tggcctgaag
     2701 tactgtgagt ttctaaacaa gcagtacggt actgactata tctctgttat gcctactaac
     2761 ctgtatggac ctaacgacaa ctatcatcct actcacagtc atgtgctccc cgctttgatt
     2821 cgtcgcttcc atgaggctaa ggaagcaggt ctgcctactg ttacctgctg gggtgatggt
     2881 tctcctctgc gcgaattcct atatgttgac gatctggcaa acctgtgtgt gttcctgatg
     2941 aataactata gcggtgacga gactgttaat gcaggtactg gcaaggaact gtctatcaag
     3001 gaactgaccg agatggtagc aaaggttatt ggttacgaag gcgagattct gtgggatacc
     3061 agcaagccga acggtacacc tcgtaagctg ctggatgttt ccaaggcaac caagctggga
     3121 tggacatata agactgagtt ggaagatggc atccgtctgt cctacgagga cttcctgaat
     3181 aacccgatgc gtgctgaacg ctaatttacc gaggttgctg tgatttttga ggattgcagc
     3241 agcctctttc ttataaaaag gagagatcat tatgaatatt gttttactgt ctggcggttc
     3301 tggtaagcgc ctttggccgc tgtctaatga catccgttcc aagcagttca ttaagatttt
     3361 caaaactgaa gatggatatg aatccatggt tcagcgtgtc taccgccaga ttaagaaggt
     3421 agataacgac gcaactgtta cgattgcaac ctctaagaca caggtttctg ctatccataa
     3481 ccaacttggt gaggatgtgg gtgtatctgt agagccttgt cgccgcgata ccttcccggc
     3541 aatcgcattg gctaccgctt atctggtaga tgtgatgggc attgatccag aagagtccgt
     3601 agttgtttgc ccggttgacc cttacgtaga agatgactat tttgaggccc tgaaatctct
     3661 gtccgatcag gctgacaaag gcgaagctaa tctggtgctc atgggtattg aaccgactta
     3721 tccttccgag aagtacggct acatcattcc tgagagcgca gagcagactg ctttcgtaaa
     3781 taccttcaag gaaaaacctt ctgaggctgt ggcaaaggaa tatatcgcac agggtgcact
     3841 gtggaacggt ggtgtgtttg tctacaagct gaagtatgtc atggagcgtg cacatcagct
     3901 gattgacttt gtagattatc aggatctgtt caacaaatac gatactctga ctaagatttc
     3961 cttcgactat gccgttgttg agcatgagga caagattcag gtacagcgtt ttgctggtat
     4021 gtggaaggat ctggggactt ggaatactct gaccgaaggt atggaggaat ccattatcgg
     4081 taaaggtgtt atgaatgaga attgcgatgg cgtacacatc ctgaacgaac tggatgttcc
     4141 tgtgttggct atgggtctgc atgatgtggt catctctgca tctcgggaag gtattctggt
     4201 ttccgataag gaacagtcca gctatattaa gcctttcgtt gacaagttcg agcagcagat
     4261 catgttcgca gagaagtctt ggggtagtta taaggttatg gatgtagaag cagagagcat
     4321 gaccattaag gttactctga atgctagtca ctccatgaat tatcacagcc acaagaaccg
     4381 tgacgaggtg tgggtggttc tggccggtac tggtaagacc atcgtggatg gatatgagca
     4441 ggttgttaag gctggcgatg tgatcaccat gagtgctggc tgtcggcata ccattatggc
     4501 agatactgag ctgaagctga ttgaagtaca gcttggcaag gatatcaatg ttaaagataa
     4561 acagaaattt gagttagagt attaaaaaac ggaggagggc tcgttataca gaaaccgttt
     4621 ttattgaagc cttccgcaaa ggattatctg tgggggggaa ccgcctgaat gatgaatttg
     4681 caaaaggaat tgagatgtct cctcttgcgg aaacttggaa tgctctacgc acccggatgg
     4741 accgagcttt gttgtgtctg gtcagagtat caggggatga ccttgacgga ggtgttgcaa
     4801 gaacacccag agtttctggg aacgcatccg cagaccaaag gagaattgcc gatcctgatc
     4861 aaattcattg atgcgaaaaa ggatttgtct gttcaggtac atcccacaga tgaatatgca
     4921 attgaaaaag aaaacgggca gcttggaaag acagagatgt ggtatgtact ggatgccagc
     4981 ttaatctatg ggctgcagaa aataacagaa gaagctgtga tccgtgaagc gattgctcag
     5041 ggaactgttg agcgttatct tcagaaggtt ccggtcaaaa agaatgatgt gttctttatt
     5101 gaggcgggaa cgattcatgc gattggtgcg ggtgcactaa ttgcagaaat tcaggaaaat
     5161 tccaatctga cttatcgtct ctacgattat gaccgtgttg acaaaaatgg taaaaagaga
     5221 gaacttcatg tagacaaagc gctagaagta gcgaacttga aagggttgtc agaaccgaaa
     5281 cagccgcttc gtgttctaaa atatcgtgct ggtgtagctg cggaattgct ttgcagatgc
     5341 aagcatttcg aggtctctcg tatgattgtc aacactgaga gaagacagac ggtacactat
     5401 cgtgcggatt cagtcagctt ccgtgtgttg ctgtgtgtca atggctgtgg taccgttcga
     5461 tttgataatg aatcgttgga catctataag ggggactgcc tttttgtgcc ggccgattct
     5521 actgaaatca gcattcacgg gtcgattcag tttctggatg tgagaggata ataatatgaa
     5581 ttataaagta gaatatgagc gttggctgaa tcatgccaat gcagatatta tagaagaact
     5641 gcgttcctat tctgagaagg acattgagga ctctttctat aaaaatttgt ctttcggtac
     5701 tggtggtctg cgtggtacca tcggtgcagg cactaatcgc atgaacgtac acaccgtagg
     5761 taaggcaagc caaggtctgg cagactatct gaataagaac tatgaagccg cttccgtggt
     5821 ggttggctat gattcccgta tcaaatctga tgtgtttgca aagactgccg cagacatctt
     5881 tgctgcaaat ggtattcagg tgcacttgtg gccagaactg cttcctgttc ctacagtatc
     5941 ttttgcgact cgttatctga aggcagatgc cggtgttatg attacggcat ctcataaccc
     6001 ttctaaatat aacggctaca aggtatacgg tgcagacggc tgtcagatta ctaccgaggc
     6061 tgctactgca attctggcag aaatcgaaaa gctggacatc tttgctgatg taaagatgga
     6121 tggcgatgct tctaagattg catccattca gtcggaagtt ctgactgctt tcatcgaaga
     6181 agtgaagggg cagtccgtcc tgtttggcga aaaagtggac aagaatgttg ctatcgtgta
     6241 ttctcctctg aatggtaccg gtctggtgcc tgttacccgc accctggcag agatgggata
     6301 cagcaatgtg accgttgtgg aagaacagcg tctgccggac ggtaatttcc cgacttgtcc
     6361 ttatccgaac cctgaaatta aggaagctat gtctctgggt atggagtatg ccaagaagta
     6421 caatgcggat ctgctgttgg ccactgaccc tgactgcgac cgtgttggta tcgcagttaa
     6481 gagcgcacat ggtgactatg ttctgttgtc tggtaatgag actggtctgc tacttctgga
     6541 ctacatctgt tctcagagaa ttaagcatgg aaaaatgcct gctgaccctg tgatggtcaa
     6601 gaccatcgtc accatggatt tgggtgaacg tatcgcatcc aactatggtg ttcgtaccat
     6661 cgatgtgctg accggcttta agtttatcgg tgagcagatt ggtatgttgg aaaaggctgg
     6721 taaggctgac agctatatct tcggctttga ggaatcttac ggctatctgt ccggtagcta
     6781 tgtccgtgac aaggacggtg ttaacgctgc ttatctaatc tgtgaaatgt tctcttacta
     6841 caagactcag ggtatcagcc tgctggataa gctgaatgag atttatacaa agtatggcta
     6901 ttgtttgaac accctgcatt ccttcgagtt tgacggtgcg gcaggtttta ccaagatgca
     6961 ggaaattatg gttgaattcc acaagggtct cgatataatt ggtggtaaga aggttattga
     7021 aactttagac tacagtaagg gcctgaatgg tctgccgaag tctgatgttt tgaagtttat
     7081 gctggaagat aactgctctg ttgtggttcg tccttctggt actgagccga agctgaagac
     7141 ctatatcagc gtcagcgcag agaacaagga agccgccgaa gctatggaag ctattatggc
     7201 tgctgaactg gcggaaatgt tcgagtaaga aaagaaagga gaaatcttgt aatggaggaa
     7261 ctgttttcta acgctttgaa atcggtgaag tcaaatcgtg tgctgtacac ggcatcgttc
     7321 tttgcaagat cctctctttt gtatttgcag gaaattggac agctgactgc tctgaaggca
     7381 catacatcta ggcgtacagg attgtcatct tacctttgtt tcctggtaga agttggttca
     7441 ggcacattgg agtataaggg gcagaaatat acgcttcaag taggagatat ggtgtttatt
     7501 gactgtttga agccgtatgt ccatgagtca tcagaagatg atttgtggac tttgcggtgg
     7561 tgccatttta atggcattgg tatgacggaa gtctacaaaa aataccttga acgaggtggt
     7621 caaccggtct ttaaaacatc aaaaacagat aagtatgctg acttgctgag gagccttcat
     7681 atggtggcag agggacagga gtatgtccgg gatatgaaaa tcaatgttct actcagtgaa
     7741 ctgctgatgc atttgatgga agattcttgg aatcctgaca aagtgccgaa tttctctaca
     7801 aaaaaaatgg aattgcagaa cataaaacaa tatctggatg accattacac ggagaagatt
     7861 acgctggatc agttgagtgc agagtttttt atcaataaac agtacttagc taagattttt
     7921 cgtgaaactt acggtacaac tgttgtcggt tatctgaatc agaaacgaat cacacaggcc
     7981 aagcacttgc tacgctttac aaatctaagt atggaagaga ttgcaacaaa aattggcata
     8041 acagatgcaa actatttttc cagaactttt ttcaaggtgg aaggtgttcg tcccagcgaa
     8101 ttccgcaaga tgtggtaata aaaagccggc ggtgttgagc cgccggtcag tacctgtcgt
     8161 taacccgcat tcaaagcttt aatctcagct ttgatttcag cacgaacacg atagtaatct
     8221 tcagtttcga tttcggtatc cagccgagtt gctgtctttc cgcagtcagg acaacgctct
     8281 ggctgttcgt cggcagggaa gctgtagtgg caggaatcac aggtgtaaat gtgatcgtca
     8341 cgtataatca tcacctacca atccagtgct tagatttttc atgcaattac tattatatcg
     8401 aattggtgat tccacaaaaa tccgcttctt gataaatgta cgattcagag tgatatatag
     8461 tactgtactg ccaaagggaa taccacaacg gcagaaatgg atgattcaca tggaagaaat
     8521 tatgcagatg attttaggga acgcaaaatt ccagctcgga caggtggttt cgacccctgc
     8581 tgtattggaa actttcaacc ccctgcagac cgcaaacggc attcccgaag ccttgagcaa
     8641 cctttggttt agacaggaaa ttagtattgc ggagatcgag gacgcagttc tggatgccaa
     8701 gaacggcaag gagctagcaa acagcctcaa ccgcatcaag cttttccgca agttcaccct
     8761 cgaccgagag ctggacggca tggtcagact gaagggcgcg gacgtctgga agaacatcag
     8821 ctacctcaag gtctacaccg ccacgccgcc taagaaaccg acccccccaa ggttccagtc
     8881 gaacggctgg agccttttct tctctcataa atcggcataa tgtatacaaa tacagctcca
     8941 aatgattgtg tagtatatgc ctgtggattg acttgatata gtcgagagac tatggcaata
     9001 tacacacaac caaaggggaa aatcccaaaa acaaggagga aacgctaatg gcaatgagcg
     9061 aaaaggcaaa gaaatatttg aaggagatca agggtgcaaa gaccatcccg gagctgaagg
     9121 atgtggagat cgcaatcaag cgagacggta tccttgcttg ggcagagttc accaagttga
     9181 acgaggcggt ggaagagaag aaggtcgccc ttcgcaagaa aaagcaggag accagccttc
     9241 aggagatcct tttctgggcc tacaaaaagg aatcagacaa gctgctgaag atgatggacg
     9301 agggagcaga caaggatgcc atccagatgc aggtccagcg ttacgacagc atcggccaga
     9361 tcattgcaga ggcagacctc gaagatgagt atgaggtttg agaatacagc ctgaaccact
     9421 aagaaccaaa taccgaaagg gacttctgaa acagacagag gtccctttct tttgctcact
     9481 ttggcggtca attatgaacc aaaaaccagt cgaatgattg tgtacattat ggctttcaaa
     9541 cgacttgata ataatcactt ttagagtgat atatgtacta caaaaaacaa aagactggag
     9601 aaaacaacag atgagcgaat acagaatcat ttatatcagc gacgaggacg gcggcgaggt
     9661 ggaaggatgt ggctacacgg ctacgatggt tatgatgcgg tcagaaactt cttggaattt
     9721 gcctgcggca tcttgaaaat cctgcgggtg cgattcatca gaagagtgga ctgaaataca
     9781 agttagcaga tagagaagag agtcaggttt cgacttggct ctgttctctg tctactggca
     9841 gaaatacatt gaagggaggt gacgactgtg gcaaccagag gaagaaaatc aaccccgaca
     9901 gccatgaaga agctggaggg aaatccgggc aatcgcccac tcaatgcaaa agagtcaacg
     9961 ccggaaaaga aggcaccgac ctgtccgaag tggctggaac cagaggcaaa gaaggaatgg
    10021 aagcgtctgg caaagcagat ggaggccatc ggaatcttga cagatgtgga tatggctgcc
    10081 tttgccggtt agtgccaagc ctacgcccgc tggaaggaag ccgaggaatt cattacccag
    10141 cacggtatca tcgtgaaaac cctatccggc tattggcagc aggtaccaca ggtgtttatt
    10201 gcccagacct atctgaaaat tatgaaccga ttttccgagc agttcggtct gacgccttcg
    10261 tccagaagcc gtatcattgc cggtgatggc ggtggtgtcg gagcagaaga tgcgatggag
    10321 gctctgttgg gaggtggtag ctgatggcag aaaccagacc aaagggctat cccaaattaa
    10381 aggaatacac accaaaccga ttcatgcttt cggaatgtca ctacgacaaa gccagagcgg
    10441 atcgggcggt gaactttatc ggacagctcc gacacaccaa aggcaaatgg gcaggcaacc
    10501 gcttctggct cttgccttgg caggaacaga tcatctagtg taatctcgct taactgtatt
    10561 aacggacgta gcgactttat taagagtgtt catggtagga gcattagttg caacaatgat
    10621 tgatattaaa tttttatggt accaacgatt ggcgatgtga cattgaatat gcaaaactat
    10681 atcgatatga tattatctaa aatcttactc gctgggatcg tcgaggtgtt tattggttgt
    10741 taggaaagaa aaatatgacc caactcgagc gatttttatc gtattacttg tgtgtggctt
    10801 tatctgcatt gtgtgtgatt tcgaaataag ggagagccaa aaatgagtag aaaattgata
    10861 ttgattagtc acggaaaatt ttgtgaggaa ttgaaaaaaa gtacaaaaat gattatggga
    10921 cctcaagaga atatctacac gttaccttta ttaccagaag atggcactga aacatacaga
    10981 caaaagctct tactgtcata gattcgttaa atgattttgt ggtactatgc gatcttttag
    11041 gcggtacacc tactaatatc atttctaaac tgataatgga aggacaacag attaaacttt
    11101 atgtcggatt aatcttccta tggtcattga atttatcaac tcgcaagtga ttggttcaga
    11161 gcccgagttt gttcgtgcag gatgtgaaag cattgtatct gtcaactaat taattagtgg
    11221 aatagtagaa gatgaagagt atattatttg tcaaattaag cgagacaaaa ctttccattt
    11281 acgtaactca aaaaaacttc taatggtgtt tggtaattta gtgattttct agggatatgg
    11341 tttcttttgg acgcaacgga tgagatgaat ccttgattga cttggttgaa
//
LOCUS       DACEZN010000085        11265 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEZN010000085 DACEZN010000000
VERSION     DACEZN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.085"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(784..1191)
                     /gene="arsC"
                     /locus_tag="IXH60_001798"
     CDS             complement(784..1191)
                     /gene="arsC"
                     /locus_tag="IXH60_001798"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ1990712.1"
                     /translation="MRKIYFLCTGNSCRSQIAEGYGKAILPTDEFEIKSAGIEQHGLN
                     PRAVQVMAEEGIDISTQTSDLIDMDYFNQADLIITLCGDAKDKCPVIPKGIKHVHWDL
                     EDPAKATGTEEEIINKFREIRDDIKSRVANLLD"
     gene            1549..1908
                     /locus_tag="IXH60_001799"
     CDS             1549..1908
                     /locus_tag="IXH60_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1990713.1"
                     /translation="MKNEICEISCVHEDKVLLGKEKLQTEDISSLKSVFKILADENRL
                     KIIYALSYEDELCVCDIAATIEATVATTSHHLLSLKKNGIVVSRKVGKLVYYSIKNDK
                     FVQLLNSKLYLEDEVLV"
     gene            1905..4022
                     /gene="cadA"
                     /locus_tag="IXH60_001800"
     CDS             1905..4022
                     /gene="cadA"
                     /locus_tag="IXH60_001800"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1990714.1"
                     /translation="MSEKKQVYRVEGLSCTNCAAKFEKNVSQIPKVTDAKVNFGAGKI
                     SIEGEATIAEIEAAGAFENLKVQSEHDTEPRIETKEPFVKRNWNLLVSLFLIILALGS
                     QVVNGEDALLTEGLFILAIIIGGFSLFKEGFSDLLKLNFSMESLMTIAIIGAAIIGEW
                     TEGSIVVILFAISEALERFSMDKARQSIRSLMDIAPKEALIRRNNVEQMINVSKIEVG
                     DIMIIKPGQKIAMDGQVIKGHSSVNQAAITGESVPIEKNINDDIFAGTINEEGALEVK
                     VTKHVNDTTIAKIIHLVEEAQGERAPAQAFVDKFAKYYTPTIMVIAALIVIVPPLFFN
                     GDWNTWLYQGLSLLVVGCPCSLVISTPVSIVSAIGNSAKNGVLVKGGIYLEEIGGLKA
                     IAFDKTGTLTKGTPTVTDFTTVDSKDEEKYFSIITALESYSQHPLASAILKEADNRAI
                     SYKSVVVDEFTSITGKGIQGNIEGITYLVGSPKLFESILTDNSKIIENYQRLQQQGKT
                     AMLLGTDKQILAVIAVADELRESSKAVIEKLHDLGIEHTIMLTGDNATTAQSIGKQTG
                     VTEIKGDLMPQDKLDYIKSLKETYGKVAMVGDGINDAPALAASTVGIAMGGAGTDTAL
                     ETADVALMGDDLQKLPFIVKLSRQTLRIIKQNITFSLGIKLLALLLVVPGWLTLWIAI
                     LADMGATILVTLNGLRLMKVKSK"
     gene            complement(4243..5055)
                     /locus_tag="IXH60_001801"
     CDS             complement(4243..5055)
                     /locus_tag="IXH60_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1990715.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGTQENQYILEMLQKKAGKLDPKIEGLIVDELKQ
                     AGLVSEKEMDRMIEFFIINLIPPTEQDQFTYQCIDEKKQLYLGSTKFGKEAQLYLHHD
                     NQNHILHILPFTFSNFSVDRLLVYLANQESYSYFGKHLPKELVEKAILLYSINQLTVD
                     VRIAPLVYSKETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNVAYPSSK"
     gene            5217..6473
                     /locus_tag="IXH60_001802"
     CDS             5217..6473
                     /locus_tag="IXH60_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1990716.1"
                     /translation="MNQVNQHDLGESIRVSREERGWTQRYLAEKVGISRSLLSKVEKG
                     TRQLSAEKLNLILDSLQEEIVPVNRVLIDYLTIHFFSNQHLKLIEAIIGMPIERFEEL
                     DYAPKGYIGQYVWNQVITIRYSIDDTVKGTVMEFSGQGCKHLAMRLKTAKSNWQEFFR
                     KVLDYQGNFTRIDFTLDDFVGSLSIPELKRKVTLGHVWTTFQVSESHGGTDIINNESN
                     GETLYLGSKKSQCRFCFYQKDYEQRKRRGISLEEAEVKNRFELRYRKEKAQSLAKIIS
                     KTHDLTKLFFELLNGAICFYDRAPNDPGAKVDAKWAAFIGNHGATTISLETIPQSFEK
                     SMNWLIHSVSPTLAFIQEVDNHFDSNLINEIISCGELSSRQQKILENLIAEPDYYQEE
                     VEFYIQCLQNMKAAKIHKKSKAQLTH"
     gene            6563..6760
                     /locus_tag="IXH60_001803"
     CDS             6563..6760
                     /locus_tag="IXH60_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1990717.1"
                     /translation="MKNTITTYELPYLLTREQASHFLGIDPKSFDKFVRANDELERFM
                     IGRQERYTVTSLINFIKTHSI"
     gene            6866..7915
                     /locus_tag="IXH60_001804"
     CDS             6866..7915
                     /locus_tag="IXH60_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1990718.1"
                     /translation="MPSIIKRGNSYRAQISLYNHGQHKKLTKSFSSKKEATRWALENE
                     LEKGNGKQLAERTTTFADFFENWIHIIKKNDVKETTFQNYQRTSQVVKKLFGDIQLKD
                     LNDIVVQRKIDKYAETHSRKTTHEVLLKIKTALRDAYARGYLATDFANLVKTRGKVLD
                     KRNRVLSITELKKLRTYVINHRENEFNILVLLALETGMRRGELLGIRPEDLFEYGIHV
                     RRSLSPTSEDTSLKTKNSRRDISINQEVYDILKSVPIKDNGYFFDPDGFQQSAKLARL
                     LKKLDIPKTTFHGLRDTHASFLFSQDIDIAYVSKRLGHINIQTTQNYYLELMPEKKHQ
                     QDADALNLLNSLSNL"
     gene            complement(8213..8833)
                     /locus_tag="IXH60_001805"
     CDS             complement(8213..8833)
                     /locus_tag="IXH60_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990719.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(8914..9324)
                     /locus_tag="IXH60_001806"
     CDS             complement(8914..9324)
                     /locus_tag="IXH60_001806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990720.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            9354..10532
                     /locus_tag="IXH60_001807"
                     /pseudo
     CDS             9354..10532
                     /locus_tag="IXH60_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT         3758 a         1958 c         1976 g         3573 t
ORIGIN      
        1 aagcggataa ataatattgt ttgatgatac tttatctttt tcccgtttgt ttatagatcg
       61 aatgatatta tagaccaaga atcctaccta ttaacatagc taaaaacaca aaaaaggcac
      121 tttccaaatt gtggaaagtg ccagaaaagt taggattaag gtaactgcgc tcagcattgc
      181 ttcgcccagt acgattctcg taactcgtga actcgaacga taatcgcaat taacgttttg
      241 agatgtgact cgtacttatt acaagtactt ctatcaatag aaatccaatt atatcaacat
      301 tacaaacatc taaaaaacag tttgatatgg ataaaatagg attaatattg atattacttg
      361 aaccaatttg aaccaaagat gaatggtagt tgaatacaat tttcttacta gtttatcaat
      421 cttttttaga gttattcctc ccctcttatt tatttgagac cgtattgatg gctggatagc
      481 tgtctaatag gggtttcaag gccagaatta gctaagctac tagattctcg aataattgta
      541 taagccggtc acacctactt aaatgtgatt gagaaggtca aatatagggt caaaaaatac
      601 tcgtcagtat gtcagataga taaatccaat tttacgccaa aagcagatcc ttttctctta
      661 aaatagtaag agccatcgaa gatttatgtt tcacttcgca cggcttttac tttaattttc
      721 caatttatca aaaggacatt ttacgtcttt ttatctttta ccgagataga tgacagttgc
      781 tacttaatct agcaaattcg ctacacgact ttttatatca tctctaattt ctctgaattt
      841 attaatgatt tcttcttctg ttccagttgc tttggctgga tcttccaaat cccaatgaac
      901 atgcttaatc ccttttggaa ttactggaca tttatctttg gcatcgccac atagcgtaat
      961 aattaggtct gcttgattaa aataatccat atcgatcaaa tcagatgttt gcgtagagat
     1021 atctatccct tcctcagcca ttacttgaac ggctctagga tttaacccat gttgttcaat
     1081 gccggcgctt ttaatttcaa attcatctgt tggcaaaatc gctttcccat agccttctgc
     1141 aatttgacta cgacaagaat tgcctgtaca taaaaagtaa atttttctca ttattatttc
     1201 ctccgatatc agacaattct gatggatttt gaatgaacca atttttttgt gcgattagat
     1261 attttaacta acagtaacat gactggcact tcaactaaga tgccaactgc cggcgcttat
     1321 gtaaaaatat agataatcat ctatgttttg tcaaatgctt ctgtctttca gttcaaatta
     1381 attagtttat tatttgcatt cgattcaatc taatttgaaa atcactactt atatagatgc
     1441 atccatacaa tctatataac tcctcacata ttcaaattaa aatttgatta ttctattaat
     1501 ctatgataca ctacattcaa atatccatta gaataaagga ggagctgtat gaaaaacgaa
     1561 atttgtgaaa tctcatgtgt tcatgaagat aaagttttac taggaaaaga aaagttacaa
     1621 actgaggata tttcgagctt aaaaagtgtc ttcaaaattt tagctgatga gaatcgctta
     1681 aaaattatct acgccctaag ttatgaggat gaattatgtg tatgtgatat cgcagcaact
     1741 atcgaggcaa ccgtggcaac aacctcacac catttattat cattgaaaaa aaatggaatt
     1801 gttgtgagtc ggaaagtagg gaaattggtt tattactcta tcaagaatga taaatttgtc
     1861 caactactaa attccaaact gtatttagaa gatgaggtgc tagtatgagt gaaaaaaaac
     1921 aagtctatcg ggttgaagga ttgagctgta cgaattgtgc ggcaaaattc gaaaaaaatg
     1981 tttctcaaat accaaaagtt accgatgcaa aagtaaattt cggtgccgga aaaatctcta
     2041 tcgaaggcga agcgactatt gcggaaattg aagctgcagg agcttttgag aatcttaaag
     2101 ttcagtccga acatgataca gaacctcgca ttgaaacaaa agagcctttt gtgaaaagaa
     2161 actggaacct attggtttca ctgtttttaa ttattttggc attaggatct caagtagtta
     2221 acggtgaaga tgcgctatta acagaaggat tatttatact agcgattatt atcggcggat
     2281 ttagtttgtt taaagaagga ttttcagacc tattaaaatt gaatttctca atggaatctc
     2341 ttatgacaat tgcaatcatt ggtgctgcaa ttattggcga gtggactgag ggctcaatcg
     2401 ttgttatctt atttgcaatt agcgaagctt tagaacggtt ttcaatggat aaagcaagac
     2461 aatcaatacg ttccctaatg gatattgctc cgaaagaagc attaattaga agaaataatg
     2521 ttgaacaaat gattaatgtt tcaaaaatcg aagtaggcga tattatgatc attaagcctg
     2581 gacaaaaaat tgctatggat ggtcaagtca ttaaaggcca ttcctcagta aatcaggctg
     2641 cgattaccgg tgaatctgtc cctattgaaa aaaatattaa tgatgatatc tttgcaggaa
     2701 cgattaatga agaaggtgca cttgaagtca aagttacaaa gcatgtgaac gacacgacaa
     2761 ttgccaaaat tattcaccta gttgaagaag cacaaggtga acgagcacct gctcaagcat
     2821 ttgtcgataa atttgcgaaa tattatacac ccaccattat ggtgattgcc gcattaatcg
     2881 tcatcgttcc cccattattt tttaacggag attggaatac ttggttgtat caagggctct
     2941 ctttattagt tgttggctgt ccttgttctt tagtaatctc aacacctgtg tctatcgttt
     3001 cagcaattgg aaactctgct aaaaatggtg ttttggtaaa aggcggtatt tatcttgaag
     3061 aaattggtgg cctcaaggcg attgcctttg acaaaacagg aacattgact aaaggaacac
     3121 caacggtaac agatttcact acagttgatt caaaagacga agagaaatat ttctcaatta
     3181 ttacagcatt agaatcatat tctcaacacc ctctcgcgtc agcaatctta aaagaagctg
     3241 ataatagagc gatttcttat aaatcagtag ttgttgacga gtttacttca attactggaa
     3301 aaggaataca aggaaacatt gaggggatta cctatcttgt agggagtcca aagctttttg
     3361 aatctatctt aacagataac tcaaagatta ttgaaaatta ccaaagactt cagcaacaag
     3421 gaaaaacagc aatgctctta ggaacagata aacaaatttt agcagtcatt gcagtagctg
     3481 atgagttaag agaatcaagt aaagcagtca ttgaaaaatt acatgattta ggaatcgagc
     3541 atacgataat gctgactggt gataatgcta caactgctca gtcaatcgga aaacaaacag
     3601 gtgtaactga aataaaaggc gatttgatgc ctcaagataa attagattat attaaatcac
     3661 ttaaggaaac ctatggaaaa gtcgccatgg ttggtgatgg tattaatgac gcaccagcat
     3721 tagctgcctc aacagtcggt atcgcaatgg gtggcgctgg aacagataca gctttagaaa
     3781 cagctgatgt agctttaatg ggtgatgact tacagaaact tccctttata gttaaattaa
     3841 gtcgacaaac attgagaatc attaagcaaa atattacctt ctcattggga atcaaactat
     3901 tagcattatt acttgtagtt ccaggctggt taacgttatg gattgctatc ttagctgata
     3961 tgggagcaac tatattagtt accttgaacg gcttacgact gatgaaagtt aaatctaaat
     4021 agacaagtag tagcataaaa agaggaaaga ctagcgacgg cagcgccgta taagctagtc
     4081 tttcctcttt ttaatgctgt tgcaggacgg cgtagccgga ctgcatgggc aagcccctct
     4141 ttctaacagg gggctaacaa agaacaggaa actgtgacaa ctacccattt gggagtgtcc
     4201 tagcagtgtt ttctttgtca catgcttatc aatcttgtca cctcattttg atgatggata
     4261 cgctacgttt aacagacgtt ctttctcgac ttccatcacg aatggtagat aaagatattg
     4321 ttgttctagt agatattgat tgtcctctgt gtactgataa gaagctgttt cttttgaata
     4381 gaccaacggg gcaattcgca catcgacggt caattgattg atcgagtata gaagaatggc
     4441 tttttcaact agttccttgg gcaaatgttt cccaaaatag gaataggatt cttgatttgc
     4501 taaatagacg agtaagcgat ccacactaaa gttggaaaag gtaaagggca gaatgtgcag
     4561 aatgtgattt tgattatcat ggtgtaaata gagttgagct tctttaccaa atttagttga
     4621 accaaggtaa agctgctttt tctcatcgat acattgataa gtaaattggt cttgttccgt
     4681 tggtggaatg aggtttataa taaaaaattc aatcatccga tccatttctt tttcactaac
     4741 aagaccggct tgtttcagtt cgtctactat tagcccttca atcttaggat caagcttacc
     4801 tgccttcttt tgaagcattt ctaaaatgta ttggttttct tgagtaccat acaaaagata
     4861 ttcgttggtg acatggccta tttctgcaat ttggtgaagg gtttcggttt ttggaaggtt
     4921 aatcccacgt tcccagttat tgactgtact tcgtggtaaa tcaccaatta gtttgccaaa
     4981 tttctccata ctcaacttta actcttgacg aatctgccga attcgattgc ccactgcttg
     5041 cttgtctatc tccatgaaaa ctcctcctca tgataagttc ttaaattcag tatacgcttg
     5101 tacttaaaaa aagtcaaaat gtattgactt aaaataaata caggcgtaca atcgatttgt
     5161 acttaaaata agtctgatgg ttctttgaca atttaatagc agaaggaggt ttttctatga
     5221 accaagtcaa tcaacatgat ctcggggaat cgattcgagt ttctcgagaa gaaagggggt
     5281 ggacccagcg atatttagca gaaaaagtgg ggatttcgag atcgttatta tcgaaagtag
     5341 aaaaaggaac gagacaatta agtgcagaaa aattgaatct tatactggat agtttgcaag
     5401 aagagatcgt tccagttaat cgtgttttga ttgattatct aactattcac tttttctcga
     5461 atcaacattt aaagttgatt gaagcaatta ttggcatgcc tattgaacgc tttgaggaac
     5521 tggattatgc gccaaagggc tatattgggc aatatgtttg gaatcaagta atcacgattc
     5581 ggtattccat tgacgatacg gtaaaaggaa ccgtaatgga gttttcaggt caaggctgta
     5641 aacatcttgc catgcgtttg aagaccgcaa aatcaaactg gcaagagttc ttccgtaagg
     5701 tattagatta tcagggaaac tttacacgga ttgattttac tttagatgat tttgtgggga
     5761 gtctcagcat tccagaactg aagcgtaagg taacactagg ccatgtgtgg accacttttc
     5821 aagtgagtga atctcatggc ggtacggata tcataaacaa tgaatcaaat ggtgagacct
     5881 tgtatttagg ctcaaagaag agtcagtgtc gcttttgctt ttatcagaag gattatgaac
     5941 aacgaaagcg acgaggaatt tctttagaag aagcagaagt aaaaaatcgc tttgaactac
     6001 ggtatcgaaa agagaaagcg caaagcttgg caaagattat ttcaaaaacc catgatttaa
     6061 ccaaactatt ctttgagtta cttaatggag caatttgttt ttatgaccgt gctccaaatg
     6121 atccaggtgc aaaagtcgat gccaaatggg cagcctttat tggcaatcat ggcgcaacca
     6181 ccatttcttt agaaacaatt cctcaaagct ttgagaaaag tatgaattgg ttaattcaca
     6241 gcgtttctcc caccctcgca tttattcaag aggtagataa tcattttgat tcaaatttga
     6301 ttaacgagat tattagctgt ggggaactta gttcaaggca gcaaaaaata ttagaaaatt
     6361 tgattgctga gcctgattat tatcaggaag aagtagaatt ttatatccag tgtcttcaaa
     6421 atatgaaggc agcaaaaata cacaaaaaaa gcaaagcaca gcttacacat taaaatgtat
     6481 aaacgtactc cgccctagac aagttgagta tagcacattt ccttgtgtca gccaaaaaac
     6541 atttttggaa aggaaataaa ctatgaaaaa tacaataact acttatgaat tgccctatct
     6601 acttactaga gaacaagctt ctcatttttt agggatcgat ccaaaatctt ttgataaatt
     6661 cgttcgcgca aacgatgagt tagagcgttt tatgatagga agacaagaac gatacactgt
     6721 cacttccctc attaatttta ttaagacgca ctctatctag ctaaaatatc gtaggatggt
     6781 tgattagatg cttttcgcca tgtagaatga cggtgtattc aactagcatt tatcaaccat
     6841 ctttcagtga aaggatttgt tataaatgcc tagtataatt aaacgtggta actcatatag
     6901 agctcagatc tcactttaca atcatggtca acataagaaa ttgacaaaat catttagctc
     6961 taaaaaggaa gcaactcgtt gggccttaga aaatgaattg gagaaaggaa atggaaaaca
     7021 attagcagag cgtacaacaa catttgctga cttctttgaa aactggattc atatcataaa
     7081 gaaaaacgat gtaaaagaaa cgacttttca aaattatcaa aggacttctc aagtggtgaa
     7141 gaaattattt ggcgatatac agttaaagga tttgaacgat attgtagtac aacgaaaaat
     7201 agataagtat gcagaaactc attctcgtaa aaccactcat gaagtactat tgaaaatcaa
     7261 aacggcatta cgtgatgctt atgctcgtgg ctatcttgct accgattttg caaatttagt
     7321 caaaacacga gggaaagttt tagataagag aaaccgtgta ctttctatta ccgaattaaa
     7381 gaaactgcgt acttatgtca ttaatcatcg agaaaatgag tttaacattc ttgtactcct
     7441 tgccttagaa actggcatga gacgcggaga gcttttaggt atacgtcccg aagacctctt
     7501 tgaatatggt atccatgtca gacgttccct tagtccaact tctgaagata cctcgttaaa
     7561 aacaaaaaat tcaagacgag atatttccat taatcaagaa gtctacgata ttctaaaatc
     7621 tgtcccgatt aaagataatg gatatttttt tgatcctgac ggctttcaac aatctgctaa
     7681 gctcgccaga ttactgaaaa aactagatat tccaaaaact accttccacg gcctaagaga
     7741 tactcatgct tcttttctat tttcacaaga tattgatatt gcctatgtat caaaacgctt
     7801 aggtcatatc aatattcaaa caacgcagaa ctattatctt gaattgatgc cagaaaaaaa
     7861 gcaccagcaa gatgctgatg ctttaaacct cttaaattct ttgtcaaatt tataatttga
     7921 accaacttga accaaaaaat tcttgtaaac gttgatacaa taaggaattg attaacgttt
     7981 tgagaactgt gaagctttac gggctttctt aagacctggt taaacaaaac tctattttat
     8041 tgcattttac aggaatgtat ttcatgcgaa attaataagt ctttgtttat agagttttac
     8101 tagggtataa aaagttgtat gttatcatta cctcaaaaca ttacctcagc ttggcgagtt
     8161 ggttgattga agaataaatc atttatctag atatctcaat aaaaaatgat ttctaagaaa
     8221 cttgtatttt cgtgttatta tttttttgcc ctatcaaaat ttctaattct tctagcgtaa
     8281 tatgattatg acacataatc gattcaatta cttcaatacc tatttttttc gagaaagatc
     8341 ccttttgttc actcacaaaa atactttcag aagacatata taaggaacga agttttggaa
     8401 ttagtatatc gtacaaatag accaccataa aatcgtcctt gcaaccattc tcaaaaagaa
     8461 ttaaatttgc tgattgtgtt acatatatca ctgaattaca ttcatggtca attccaaaaa
     8521 caatgtctac ttcattttct tttctaattc gcgcagttac atttttcaaa ctttcaccat
     8581 tacttataaa tgctttagtt actgtaagct ctgatgtttg ttcagatatt ccttttataa
     8641 tattttgttc atcagccaca ttccccataa atccgccgat tccttttaag taataattct
     8701 cacctaagag tcgcttgcat tcactgaatt gataaaaaaa ccagttaaaa aaatcttctg
     8761 gaaaaataaa ttctgatcga actttattta tatttttagc tcctattaat tttataactc
     8821 gattaatgtt cacttgctgc gatgaacata taactgaata aattgagttg tttaattcaa
     8881 aaaaacaact ttaacatatg catcattaat tcgttatttt ttaggtagat attgatcatt
     8941 gttatctctc tttttttgct tcccatactc caacttagaa attgccgtta taaaatttat
     9001 tttaaaggtg actttttccc ctgaagtatc ggtagtagga atacttaata cctcagcttc
     9061 tgaggaggta tcccaattat agttatactt tgcatcatca ccataaagaa caattccatt
     9121 ttttttatct tgtgccgtta gctttactgg ttcgcttact tttcttgtta atatcttgga
     9181 ctttacatca tcgatattgc ttattttatc aagcttaaat aatttacaaa ttaatttcgt
     9241 actagttttt atatttgacg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc
     9301 ccttctgtag aatagagtta ccacaacaca ttcacagaaa agaggacttc catatgaacg
     9361 attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc
     9421 gtgtccattt ggaaaaagct gtcaatacgc ttctcaaaac ggagttaacg gctttcctcg
     9481 attacgaaaa gtacgatcgc attggtttta acacggggaa ttctcgtaac ggctcctatg
     9541 accgtacggt caagaccgag tacggggaac ttcatctcca gattccgcgc gaccgcaacg
     9601 gcgagttcaa gcaacagact gttcctgctt atagacggac gaatgatacc ttagaggaga
     9661 ccgtcattca cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga
     9721 aaatgtatgg gcatcactac acgccccaaa ccatgtccaa catgactaaa gtgctgactg
     9781 aagaagtaaa tgcatttaaa gttagaactc taaatgataa gtatgtcgct attttcatgg
     9841 atgctactta tattccttta aaacggcaaa ccgtctccaa agaagcaatc tatattgcca
     9901 ttggtatacg agaagacggc actaaagaag tactaagtta tgcgattgct ccgactgaat
     9961 cgacctacgt ttggaatgag ttgttacagg acatttactc cagaggggtt catgatgtct
    10021 tactgtttat taccgatggc ttaaaaggca tgaaagatac gattcaccag atttatccta
    10081 aagctaaata ccaacattgc tgtgttcacg tttcccgtaa tattgctcat aaagtacgtg
    10141 tcaaagatcg aaaagaaatc tgtgatgatt ttaaggctgt ttatcaagct agctcaaagg
    10201 aagaggcaaa taccttttta gggagtatga ttgagaagta gcaaaaaact tatcctaaag
    10261 tgacgcagtc actgataaaa aatcaagatt tattgacttt ttatgagttt ccgcctggta
    10321 tccgccgaag catttactca actaatttaa tcgaatcttt caagaaacaa atcaagaaat
    10381 acagccgcag aaaagagcag tttcaaaatg aagaatcact agagcgtttc ctagtatcca
    10441 tctttgatac atacaatcaa aaattcttaa atagaagcca taaaggcttc caacaagtga
    10501 cggatacatt agcttcaatg tttactgagt aacccattat tttgcagaag gacgagttat
    10561 ttacacaaaa ttattgacgc tccctccaaa ttacttcatc aaaacattga tataattttt
    10621 attatatcag tatttattct tgatacataa atgagaaaat aaatacttta aatctattga
    10681 ctacaagtag tcattaagtc ttcataagat gtacaatatg acattaaaaa aataaaagct
    10741 tacgaccatc cgttaaatct gacactaaat aatggactgc tttcttttgt attagcaggt
    10801 tacgttgact atgaatattc accagattat ttggagaaac ctcttcaaca cgagaacaat
    10861 gatattaatt ttagatttgt ctacgagtca aaaagtaaaa catggttagt tgatcaatta
    10921 agcacgataa acacaagctt ccatgaatta cctaaacctg aaaatgaagt tgagatggtc
    10981 attaaaatct tctaaaaaag aagcgttgaa ggggaaatat tcctttcaac gctctttttt
    11041 atatgcttag aaacataaat aaattgtata ctttcttttt agcccctatt acttttcacg
    11101 aaaaatatct ttttttcgac gctagcgttt ctctacttct aatactgtac acagtttttg
    11161 tattattgaa aaattactca ttatttagca ttaatccaca tcgttttatc aatcgacctt
    11221 catactaaac tactttaaag tcatcttcct tctcgttttt catta
//
LOCUS       DACEZN010000086        11164 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEZN010000086 DACEZN010000000
VERSION     DACEZN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.086"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            126..1385
                     /gene="lysA"
                     /locus_tag="IXH60_001808"
     CDS             126..1385
                     /gene="lysA"
                     /locus_tag="IXH60_001808"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1990721.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1372..2172
                     /gene="dapF"
                     /locus_tag="IXH60_001809"
     CDS             1372..2172
                     /gene="dapF"
                     /locus_tag="IXH60_001809"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1990722.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2175..3056
                     /locus_tag="IXH60_001810"
     CDS             2175..3056
                     /locus_tag="IXH60_001810"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1990723.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3053..3769
                     /locus_tag="IXH60_001811"
     CDS             3053..3769
                     /locus_tag="IXH60_001811"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1990724.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3775..4470
                     /gene="dapD"
                     /locus_tag="IXH60_001812"
     CDS             3775..4470
                     /gene="dapD"
                     /locus_tag="IXH60_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1990725.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4486..5487
                     /locus_tag="IXH60_001813"
     CDS             4486..5487
                     /locus_tag="IXH60_001813"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1990726.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINENRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5484..6845
                     /locus_tag="IXH60_001814"
     CDS             5484..6845
                     /locus_tag="IXH60_001814"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1990727.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6805..7956
                     /locus_tag="IXH60_001815"
     CDS             6805..7956
                     /locus_tag="IXH60_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1990728.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7931..9100
                     /locus_tag="IXH60_001816"
     CDS             7931..9100
                     /locus_tag="IXH60_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1990729.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9275..10654
                     /locus_tag="IXH60_001817"
     CDS             9275..10654
                     /locus_tag="IXH60_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1990730.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10935..11164
                     /locus_tag="IXH60_001818"
                     /pseudo
     CDS             10935..11164
                     /locus_tag="IXH60_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3722 a         1948 c         2497 g         2997 t
ORIGIN      
        1 aaaatatttc gaaatatact agacaattca gaaaattttg tttaatatta ttccaataaa
       61 attcaaatct ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg
      121 aaaaaatgac agaattgata attggcggag tcacagcaag cacattggca gaaaagtata
      181 agacacctct gtatgtgtat gatgaagaaa agatgagaca aacgatgctg tcattcaagc
      241 gaggctttgt atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa gcattccaaa
      301 cgattgagat gctgcattta gttcgtgaat atggattcgg gctagatgtt gtcagtggtg
      361 gggaaatcta taccgctttg caagctgatt ttcctagaga tactatctac ttccatggta
      421 acaacaagac gccagaggaa ctgatctatg cacttgaaaa tgatttgttg catattgttg
      481 ccgataattt gatggaagtt gaattattag cagaattaag ccaacgctat ttaaaaaaaa
      541 tctgtatcat gcttcggttg aatgtcggag tagaagcaca tactcatgaa tatatcgtca
      601 ctgcgcatat tgattcaaaa ttcggcatga gctatgaaag taaagagtgc caacaagccc
      661 ttcagttgat aaaagaaagc agctatctag agttagaagg atttcatgct catatcgggt
      721 cacaaatttt tgatgttact gcttggttag ctgaaatcga caaattgacg aattacttaa
      781 ccgattttga ccaacctctt tccttgaatc ttggcggagg gtttgggatt cgttatacac
      841 aaaatgatca gccgatccca gtcgaagagg cactaaaaaa actgattgaa tatacggaag
      901 aagctttgga aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg ggtagaagta
      961 ttgttggaga agctggtaca acactataca cgattggttt catcaaaaag acaccacata
     1021 aaaaatatta ctttgtagat ggcgggatga cagataatat ccgtccagct ctgtatcagg
     1081 cagaatacga ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga
     1141 ttgcaggaaa aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat ttgccgaaag
     1201 cagaatcggg tgatctttta gctgtttatt ccacgggagc ttatggatac tcgatgagca
     1261 gcaactacaa tcgagcgaca cgaccagcag ttgtttttgt caataatggc caatcaagac
     1321 tgattgttcg cagacaagca ttttctgatc tactaagggg ggaagtttcc tatgatcctt
     1381 cataaatata atggctgcgg aaatgatttt atcttattag attacgcaga agacacagat
     1441 tatagtcaat tagccgcttc tttatgcaca aaagaaaaat atgatataga cggattgatc
     1501 gccgtaaaaa tcgatccgtt ggaaatgatc tattacaaca aagatggcag ccgcgcgccg
     1561 atgtgcggaa atggcattcg ctgttttgct cgatacgtaa atgatcagaa catgatgaat
     1621 gaacaggaat tcaacgtaga gacattagca ggcacaagaa aaattacggt gttggatccc
     1681 tcaccttttt attgcagcgt agatatggga gtgccagatg attctccgga aaaaatcaaa
     1741 gctaatcaaa cttatccaat aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg
     1801 atcactagtt tatttttagg aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag
     1861 gaagtctgga gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc
     1921 aatgtcaact ttgtagaagt agtcaatgaa caagaattga tcgtccgtac ctatgaaaga
     1981 ggagtaggtt ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag
     2041 gaaactggga aaatttcagc agatcaggta gttgttcact tggaacaagg agacttgatg
     2101 atctctggtg aggatgcgat acaaatggct ggcccagcag ttcatgaatg gacaagaata
     2161 ttggaggaat gaagatgata aaaggttcga tcgtggcgct gatcacgccg atgaatgaag
     2221 agggatcagt agattatgct ggtttggaaa aattgatcca attccattta gatgaacaaa
     2281 cagatggatt attagtttta ggtacaacag gagaatcttc tacgctcaca caatctgagg
     2341 aggagcagat ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag
     2401 gagctggaac gaataatacg aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg
     2461 gggcagacgc acttttggtc atcacccctt actacaacaa aacaagtgat gcaggactag
     2521 ctgctcattt tacagcaatt gcagaagcct caccgattcc gcttattttg tataatgttc
     2581 cttctagaac aggcatgtcg attccaatcc atgtattagt aaatttagca gaacatccta
     2641 atatcattgg tttgaaagaa gcctcaggag atatggctta tgtgatggat gccgctcggt
     2701 tgattggaga agaattcttc ctttacagtg gaaatgatga tttgatccta ccagtaatga
     2761 gtgtaggagg aagcggtgta ataagtgtat gggcgaatat ccaacctaaa atagtccatg
     2821 aattagtaaa agatacgcag gacggaagat ggcagcaagc aaaagaaaag caattaaatg
     2881 cacttgaact gatccatgcc ttgttcagtg agaccaatcc tattcctgtc aaagcagcca
     2941 tgtcattatt agacttacct tccggacctc tacgcctgcc tttagtttct ctttcaaaag
     3001 agaaaaagaa acaattagct cagcttttgt taaaagaaag gacggttgaa aaatgaaaat
     3061 cggactgatt ggcagtggac agatgggaaa tagagtcgaa gaagttgcac aagaaagagg
     3121 agatacggtc cttcttttac ggacggcacc taaagaagca actacgcttg cttttacgga
     3181 actgccagaa ctgcttattg atttttctca tccagataat ttagagaaaa tcctttcgta
     3241 tagcttatca tatcaacttc cgttagtcat tggcacgaca ggttatacac aatcacagtt
     3301 ccaagaaata aaagaacatg ccaaacatgt acctgtgatg tatagtgcta atttttcgtt
     3361 cggcatcatg gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc aaggctggca
     3421 gatcgaactg attgaaaaac atcatagtcg taaaaaagat gcaccatctg gtactgcaaa
     3481 tatgctgctt caaacaatcc aagaagtgca aaagctgcag cccgtataca actggcaagg
     3541 gaaaaaaaga gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt ctttacctgg
     3601 tgaacatgat gtattgtttg ctgctactga tgaaatattt acgatcaaac atgaatcttt
     3661 ttctaatcga atctttgctg caggcgcagt agaagcagcg gactggttga aagatcagtc
     3721 accaggttat tacaaattag aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa
     3781 gctcaagaaa tcatccgcta cattcaaacg gcatctaaaa agacgcctgt gaaagtctat
     3841 ctgaatgtaa ccgagccaat cgaatttaaa gaaagtaaag tttttggtga aggaagcagc
     3901 ttcgttgttt ttggtgacta tgagacgatt gctccggttt tagaagctga atcagaaaaa
     3961 atcattgaaa tcgaaatcga aactgctgca agatacagcg cagttccttt gctagatatt
     4021 aaaaaaatta atgctcgtat cgaaccaggt gctattatca gagatcaagt gtcaatcggc
     4081 aacaatgccg tgatcatgat gggagcgatt atcaatattg gtgcagtaat cggtgaaaac
     4141 acgatgattg atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa aaactgccat
     4201 attggtgcag gagctgtctt agcaggtgtg atcgaaccag catcggccaa accggttatc
     4261 gtagaagacg gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga
     4321 aaagatgcag tcgtggcagc aggagcagtg gttttagaag atgtggcagc agaaactgtt
     4381 gtcggtggaa tacctgcacg tgtattaaaa ataagtgatg atcagacaaa agaaaataca
     4441 gcattgattg ctgcgttacg cgaattatag aggagatgga gaaaaatgcg cgaaatagat
     4501 acctgtattg tgggggcaac cggattagtc ggccaaacga tgctcaaagt gttagaagaa
     4561 tatgattttc cagtaggacg attgaaatta ttagcttcaa aacgttcagc cggtaaagtt
     4621 tgtgtattca agaaaaagaa acacactgta caggaattga cagaaacttc atttgaagga
     4681 tatgatttag cgctattttc tgcaggtgct tctgtatcca aagagtttgc gccaatcgca
     4741 cgaagtcagg gactatttgt catagataat tcctcagcat ggcgggaaga ttcggatatt
     4801 ccattaatcg ttccagaggt caatctggaa gactatacat tgaatcggct gattgcaaat
     4861 ccaaattgtt caaccattca agcggtactt ccgcttaaag ctttacaaga agcctttggt
     4921 atccagcgag tcaattattc gacctatcaa gcagtttctg gttccgggca aaaaggcatt
     4981 gaagatcttc atcgtacgca aaatgacgag gaaccagttt tgtatcctta taatattagc
     5041 caaacggtga ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa agaagaacag
     5101 aaaatgataa atgagaaccg taagattctg catcagcctg ctttgccagt atcagcaacc
     5161 tgcgtacgtg tgccaatcga aaatggacat ggagtctctg tggcagttca actgcaaaaa
     5221 gagtttacga cggaagaaat tagagagtgt ctcagccagt ttccagggat tcaattagaa
     5281 gatgatctag cagcacataa atatccgacg agcattcttg ctagaggaac agatatggtt
     5341 tatgttggga ggatcagaaa agacacttct ttagagaacg gattgttgtt ctatacgact
     5401 gccgataaca tacgcaaagg agccgctgcg aatgccgtac agattgctgc agctttaatg
     5461 cagaaaatag cggaggtggc aagatgagta atacgctgaa agtagcgaaa ttcggcggaa
     5521 catcgatgtc tcatgaagag caattcaaaa aagtccgtac aattgttctt tcagatccca
     5581 atcgccaagt gatcgttgtt tctgctttag gcaaacgtac gtcagatgat gacaaagtaa
     5641 cagacttact ttatttgatt tatgcttacc ttcagcatca tgttgattgg acaccccttt
     5701 ggaaaaggat ttgtgatcgt ttcattcaag taagagatgt tcttgccctg cagttaccta
     5761 ttgaagatga actagcacgc atacagaaac aactggaaga cgaaaagata actgaagatt
     5821 atttagtaag tagaggtgaa tatctgactg ctcaactgat ggctgagtat cttggttacc
     5881 agtttattga cgcaaaagac ctgattttct ttgactatga tgggaaaatc gatcaggaaa
     5941 aaacagcaca ggaaatcaca aaagcattta aaagatatcc gaaaatggtt gttccagggt
     6001 tttacggggc gaaccctgca ggaaaagaaa aattattggg ccgtggggga agcgacatta
     6061 ccggcgcgat actggcgaag atgttgcaag cggaaagtta tgagaattgg acggatgttt
     6121 caggaatcat gatggctgat ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct
     6181 cttttagaga actgagagag atggcttata tgggggctaa cgttcttcat gaagcagctg
     6241 ttttcccagt tcaagaggct gatatcccca tccaaataaa aaataccaat gatcctttcg
     6301 ctcaagggac aaaaatatcg aatcacgaaa tagaaaagga aaatggactg acaggaatag
     6361 ctggacgcaa ggattttctt tccatcactt tattcaaacg tcatatgtcc gatgaaatcg
     6421 ggtttatctg gaaagcaatg agcatttttg ctaaacatga gatatcaatc gaacatattc
     6481 cttcagggat cgacaatatc ggagtgatcg tctcagcaga agcgattgcc gataagttat
     6541 ttctcatcac gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag
     6601 acttagcttt gatctctgtc gttgggggtc cgcataaaga attgattgga ttatctggga
     6661 aagtgttatc gatattgaac aaattagaaa ttcgcacatc gatcttatca caaggagcac
     6721 aggagttgaa tctgatcatc ggtgtaccga ataatcaata tgaaacagta gtgaagggta
     6781 tctatgaagg gatggtgaat acggatgctg aaagaatatc gaatggcact ccatcaaatt
     6841 cctgaaatcg ggtttcatga attcaagaca aaaaaatatt tatatgaaca agtaaaaaac
     6901 agcgaggccg ttatccatga aatcggcgaa acaggactac tcctttattt tgataatcag
     6961 caagaagaaa cgattgcttt tcgaacagat atcgatgcac tgccagtcaa agaagaaacg
     7021 aatctctctt ttgcttctaa gcatgaaggt tatatgcatg catgtggtca tgacggacat
     7081 atggcgatgc ttttagggtt gactgacttt ataactaaac acattgagga tcttatttat
     7141 aatgtagtac tgatctatca gccatcagaa gaaatttccg gtggcgcaca aagcgttatt
     7201 cagtcaggat tgttggataa atatgaagta caagcaatat ttggctttca tctatggcct
     7261 ggactagcta agggagaagt tttctctcga ccaggacctt tgatggcaca aagcagtgaa
     7321 acagacatta tcattaaagg ccagtctgca catattgctt ctagtgaaaa aggaatcgat
     7381 agtctggagg cagctgtacg atttttgaag aatgtttatg attttgaccg gcaattaccg
     7441 gcagagtgga atcatctaat gaaatttggc caaatccaag gtggaacgat ccgaaatgtt
     7501 ttagctggag aagttattgt atccggaagt atccgttctt acagttcaga tatgcaaaat
     7561 tatttgaaga ggggattaga ccatttagca gaacagtttc actctacagc tactatttct
     7621 tttcgctata atgacggcta tccagctgta atcaaccata gaggcttata tgcagcactg
     7681 ttagattcaa acaaactaca tgaattagat gctccagttc ttcaagccga agattttggc
     7741 atatatacgc aacaatatcc atgtgtattt ttctttttag gtgtcggtga tacccctgct
     7801 ttacatgatc cgacgttcga ttttgatatg gatgttttag aaaatggtgt agaatggtac
     7861 aaaacgattc tttttacaga aaatcttctt gtaaaaatcg atagcagttc aaaagaggag
     7921 cgagtaaata atgatgaatt tatccaatcg atttagtcct aggctttcta gaatagaagt
     7981 atcaaaaata cgacaattcg atcagcaaat atcgtcgata ccagatgtga tcaaactcac
     8041 cctgggagaa ccggattttc caactcctga acatgtcaag caagcaggaa tagcagcgat
     8101 tgaagaagac ttttctcact ataccggaat gagaggatta gaggaactgc gggaagcggc
     8161 ttgtatattt cagcaacagc gttatggatt aacttatgat ccgcaaacgg aagttttgac
     8221 gacagttggt gcaacggaag cgattgcctc tgcgttgtta tctgttttgg aagaaggaga
     8281 caaggttctt attccagcgc ctgcttattc aggatatcag ccattagtgg aactagctgg
     8341 tgctgaattg atcccgatag atacttctga cacagggttt gtctgtcagc cggaacaatt
     8401 tgaacgagca tttgaacaat atggatcagc tgtcaaagca gtcattttaa actatcctaa
     8461 caatcctact gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa
     8521 aaaatatccg gtatttgtta taagtgatga agtatatgct gaattgactt atagtggaac
     8581 gcatatgtcc atagctactt atcttccaga acaaacaatc gtgataagcg ggttatccaa
     8641 gtcacatgcg atgactggtt ggcgagtggg ctttattttt gctcaaaaag cccttattga
     8701 cgaactaatt aaagtccatc aatatctggt gactgccgct acaacgatgt cgcaaaaagc
     8761 tgcggctgaa gcgctaataa atggggtgaa agatagcgag aatatgaaag agcaatatca
     8821 aacacgaaga gactatctgg tccagcaatt ggcgccttta ggatttgaag tgacccagcc
     8881 aaacggtgca ttttacttgt tttgcaaact cccagctgcc attcaaactg actcttggca
     8941 gttttgtgta gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg
     9001 cccagaagga gaaggttata tccggatttc ttatgctagc agcatggaaa aattacatga
     9061 ggcctgcaaa cggatcaaag actttttaac agaacagtga ttcatagatt ttatcaaaaa
     9121 aacaatcgtt aattttgcgg tatattctgc aaggagacga ttgtttttta aatttagttc
     9181 aaatgaacgc gtttcttttg attcataact aatgtaatgt taagtttaaa ttgtttagaa
     9241 tgattttaaa agaatattca aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt
     9301 aggtgcatct catggagggc atcaatcgat cttggaacta ttgacacgat acggtgaaaa
     9361 tgtagatatc acgctatttg aagcaggaga ctatatttcc ttcatgtcat gtggtatgga
     9421 gctgtattta gaagacaagg taacagatgt aaatgatgta cgtaatttca gaccagaaaa
     9481 ctttccgcaa tccaatgttc atattttaaa taatcacgaa gtaaaaacga tcgatgccga
     9541 taaaaaaaca gtaacggtta ctagcgcatc agataacgaa acaaaagaat atccttatga
     9601 caaattgatt ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag gagcagatct
     9661 agaaaatgtc tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa
     9721 tgatccggct attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc
     9781 agaagcaagc cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg atagaccttt
     9841 aggcacttat ttagatgctg agatgacaga tattttagaa caacacctaa atgaaaacgg
     9901 tattgaagtt ttgacaaatg caaaaatcga agcttttact ggtaacggaa aagttgaggc
     9961 aatcaaaaca agtgaaaaag aaatcccagc agatctagtc attcaagctg ccggtgtgaa
    10021 ggcaaataca gaatggttaa aagggatcgt cgacttagat gaacgtggct ggatccaaac
    10081 aaatgaatac ttgcaaacga atcttccaga tgtgtatgca gtaggagatg ctacacttgc
    10141 ttattctatc ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga
    10201 agcacgttat gtagtaaaac atctttttga agaagtacca aacaaaccgt tcggcggagt
    10261 cgtaggttcc tctgcattaa gtgtctttga ctatcatttt gcagcaagcg gattgaatag
    10321 ctttacagca gcaaaagcag gagtagatgt acgagtgtct tattatgaag atacgttacg
    10381 accaaaatat gtaccagaag aacaaggaaa tccaaaagtg tccatccaat tattctcgga
    10441 tccaatgacc catcaattgc taggaggagc agtattatct acctatgatg ttacaggtca
    10501 aggaaatgta ttggcactag cgatccaaaa caaactgact ttagaagaac tagcagaagc
    10561 tgacttcttc ttccagccag gctttgaccg acaatggagc atgttgaatc tagccgctca
    10621 acaagcgtta ggagaagaac catttgtaga ataagaaaac gaaatatttg aaaagaggtt
    10681 gtgaaaaagc tgccttgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
    10741 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa
    10801 aaaagggact gtgacacgat ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac
    10861 ttttggaggt cggtattttt tatggttaaa tatacttttg aacttaaatt aaagatcgtt
    10921 catgattatt tggatggcaa aggcgattgg accgtaaaaa tccagatcaa gagattgaag
    10981 cggaaatgct taaaattcgg gagaagcaca aggattatgg ctgtttacgg atgaccaatg
    11041 aactaagaaa ccgcggtttc tctatcaata aaaagaaagt tcagcgcttg atcaagaagc
    11101 taggaataaa agtaacggca tacacgcgta aatcacgtcg ctacaattct tatcgaggaa
    11161 aagt
//
LOCUS       DACEZN010000087        11142 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEZN010000087 DACEZN010000000
VERSION     DACEZN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.087"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(174..1361)
                     /locus_tag="IXH60_001819"
     CDS             complement(174..1361)
                     /locus_tag="IXH60_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1990731.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            1683..2525
                     /locus_tag="IXH60_001820"
     CDS             1683..2525
                     /locus_tag="IXH60_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1990732.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            2522..3472
                     /locus_tag="IXH60_001821"
     CDS             2522..3472
                     /locus_tag="IXH60_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1990733.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            3487..3720
                     /locus_tag="IXH60_001822"
     CDS             3487..3720
                     /locus_tag="IXH60_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1990734.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            4051..4635
                     /locus_tag="IXH60_001823"
     CDS             4051..4635
                     /locus_tag="IXH60_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1990735.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            4766..5434
                     /locus_tag="IXH60_001824"
     CDS             4766..5434
                     /locus_tag="IXH60_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1990736.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            5436..6860
                     /locus_tag="IXH60_001825"
     CDS             5436..6860
                     /locus_tag="IXH60_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1990737.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            6878..7447
                     /locus_tag="IXH60_001826"
     CDS             6878..7447
                     /locus_tag="IXH60_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1990738.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            7557..9311
                     /locus_tag="IXH60_001827"
     CDS             7557..9311
                     /locus_tag="IXH60_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990739.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            9265..11037
                     /locus_tag="IXH60_001828"
     CDS             9265..11037
                     /locus_tag="IXH60_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990740.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVTMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT         3831 a         1570 c         2047 g         3694 t
ORIGIN      
        1 taatgaagag aatttaaaaa aagcctatgg agatactata tttattggag agactaacta
       61 tatagagtca agtaaaaatt agccaaatca aaaaatattt tataacagat aattccgaaa
      121 aaagggtaca acaaataagc ttcttaccat tagttttttt gaatgttcaa tagctaatca
      181 gaaacaatta tataaggctc gtttttagtc aaaatagcat gaatcacgta acaaagctta
      241 cgagaaaccg cacctaaggc agttagatga tgttttcctt cggaaacttt tttctggtaa
      301 aacgcactaa aaacaggatc acattgagaa gctactaaag ccgccgaaaa caaggctttt
      361 cgcaaatatg gagaccctcg tttactcata acattatgcg ttgcctcata ttgccctgat
      421 tgtgtcacgg aagcgtctat cccagcgtaa gcaacgagtt ttttcgggtt agaaaatttt
      481 tgaatatctc cgatctcacc aagaatagtc gcaccgttta taggaccaat tccaggaatt
      541 gtgagaataa cagaatttaa agtgtccatt atgtgcttga tttctctttc cgtttccttt
      601 atttggcttt caatgaattt taattgttca atcattgaac gtagttggaa ggtgaacgct
      661 tcttgagcaa attttactcc aaaggagtta gaagctgctt gcttcaattg gtccgctttt
      721 tctgccttga ttcggttcct gcttaactca gctaagtatt gggccaaagt ttctgaggaa
      781 atgttttcta agtcgatcgg ggcactaaac gccagcaata actctttaga agcttttccg
      841 aaaacgccct gtttggtaaa aatcgtcgcg tattctggaa agacttgatc caatacggct
      901 ataatttttc ttttgaagtc gctcgctgta cctactaaat aagttcggta acgagaaagt
      961 tgtttcaaag aaaaaacatt ttcatcggat aagaccgttt cgacaaatga gccatagcgc
     1021 attaagtcag caatcaaaac agaatcaata atatcgtttt ttcgtttccg tatttctgta
     1081 cctttacgcc atccgtctgt ttgaagggga ttcacaacgt gaatcaaata atctgattcg
     1141 tgaaggtatg aaaagatgga cagccaatag tgccccgtgg cttccatccc tataacaaag
     1201 tcactgggtg ctccggaata gtcgaccatt cgctcaatta atgactctgc tccttcagtg
     1261 gtgttaggaa atgaaaaacc ttttagtaat acctttcctt cctcgttcat gatagaagca
     1321 acgtgagtgc gtttaccgat atcaatgcct aaaaagtaca tgtatatctg acctttcaaa
     1381 aaaagaattt tggattgaaa tccactcaac tgaaaagcgt cactaccttg tacgagatac
     1441 ggagtaaagt aaggactttc aacatctaac tcatacgcaa tacacttatc agagagagga
     1501 gccagtctct cgagtgcggg ttcaatgacc caagggggga acggcttagc ctctcaatcc
     1561 aataaatata ttatctcaaa agaaatgagt taataccaaa ttaaaataac gatggttcat
     1621 agggttatcc aatcaaaacc ggaaggttaa aaccctaact ctaatataca agggggaata
     1681 atatgcttca aaattttatt aatggtctgt atgactttca ctttttacag aatgctctaa
     1741 ttacatctgt agttataggg attgtttctg gaacagtagg ttgttttatt attttaagag
     1801 gaatgtctct tatgggtgat gctatttctc atgctgtttt gccaggagta gcaatttctt
     1861 atattttagg tattaatttt tttattggtg cgattgtatt tggtctatta gcttctatca
     1921 ttattacttt tataaaaagc aatagtgtaa taaaaggaga tacagcaatt ggtattactt
     1981 tcagttcttt tttagcatta ggagttatcc ttataggagt tgcaaatagc tcaacggatt
     2041 tatttcatat cttatttgga aatattttag ctgtacaaga tattgataag tggattacaa
     2101 ttggcgtttc tgttttagtg ttggttacag ttgttctatt ttttaaagaa ttattaatca
     2161 cttcttttga tccattaatg gcaaaagcaa tggggatgaa tgtcagtttt tatcattatc
     2221 tattaatgat tctattaact ttagtagcag taacagctat gcaaagtgtg ggaactatat
     2281 taattgttgc aatgttaatt acaccagctg ctacagcata tttatatgca aacagcttaa
     2341 aaactatgat atttatttcg gcgacagtcg gagcgtgctc atcattgcta ggtttgttca
     2401 ttggttatag ttttaacata gcagcaggat caagtattgt tttaacagcg gcattcttat
     2461 ttgtaattag ttttctaatc tcaccaaaac aaaaattttt aagaaagaag gagagaaagt
     2521 tatgatgaga aaatggaaag cagtactggg aagtctgggg attttgattg ctctttttat
     2581 attcggggca tgttcaacaa atagtaaaga caaagataca gtggcttcga acgaaaaatt
     2641 aaaggtagta gttactaatt cgattttagc agatattact gaaaatatag caaaagataa
     2701 aattgattta cacagtatcg tacctattgg gaaagatccc cacgaatatg aacctttgcc
     2761 tgaagatgtt caaaaaactt caaaagcaga tttgattttt tataacggtg ttaacttgga
     2821 aactggagga aatgcttggt ttacaaaatt agtaaaaaat gcgaacaaag aggaaaacaa
     2881 agactatttt gcagcaagtg atggcataga tgttatttac ttagagggtc agagtgagaa
     2941 agggaaggaa gatccccatg cttggttaaa tttagaaaac ggtattattt acgctaaaaa
     3001 tattgaaaaa cagttagcgg aaaaagatcc tgataataaa aaattctata aagaaaatct
     3061 agataagtat attgaaaaat tggattctct agacaaagaa gctaaatcta aatttgcttc
     3121 aattccgaat gataaaaaaa tgattgttac aagtgaagga tgctttaaat atttctcgaa
     3181 agcgtataat gtgccttctg cttacatttg ggaaatcaac actgaagaag aaggaacacc
     3241 agatcaaata aaacacttag ttgaaaaatt acgcacaaca aaagttccct ccttattcgt
     3301 agaaagcagt gtggacgata gaccaatgaa aacagtatca aaagatacca atattcctat
     3361 ctattcaacg atttttactg attcaattgc agaaaaagga caagatggtg atagttacta
     3421 tgcgatgatg aaatggaacc tggataaaat tgctgaaggc ctttcgaaat aatttggagg
     3481 gtaggtatgg tacaaattaa aacgactact atactaattt atttactagc aattcaggaa
     3541 ttatcaaaaa gaaaaaagaa attaaaaaat aatgatttag caaaagttct aagtgttagt
     3601 ccagcatctg taagtgagat gctggctaaa cttagatatg aggactattt agatatagga
     3661 tttcaattaa ctgataaagg gaaaaaattt attgacgatt acaaaaaagg ttttaagtaa
     3721 aagactttat ttttagggag gataagaaaa tggctattgg agtgattatg ataggactta
     3781 ccctaatagc tggatataat ttttttaaat ctttctcgga ttattttaga aaaggtgaat
     3841 gatttagatg agtctattaa ttcttgtagg attgcttttt gtatcagtta ttttgctctt
     3901 tctcattagt ttagctctaa ggaatgagtt cagtaataga cgttaacgat tgaaacttca
     3961 agtcctgtgt aagataatat agttaattca tcttattagg aggaagtacc catagaaacc
     4021 agagattata ttacaactca ccaaaaatta atagaaagtg gactaagcca ttttgaaaaa
     4081 gaaggatttc agcaaactaa gataaggcaa ctgtgcttga ccgcaggcgt aactacgggt
     4141 gctttttata aacattttac ttctaaggaa gcgttatttg aagaaattat tcagccgcat
     4201 attaatgcgc ttattaatat gtataatgaa gagtttacca aatacctctt aaacttagaa
     4261 aatatagata tagagaaaag ttggagagag aacttcaatg aattagatcc atttattaat
     4321 tatatttttg agaataaacg tatattcgat ttggttttat tccaatcagc tggaaccaaa
     4381 tatcaagata ttgttgaaaa aattacatca tttgtgacaa aaaagacagt ctgttgtcta
     4441 gataagttaa agactatcgg aatgatcaat caaaatttag tctttaatga agaagaaatc
     4501 cacttctaca tttactcatt ctacgctaca ttctatgata tattgaagca tagctattca
     4561 aaagaaaaga ctatgctatt aaccagaaga ttgtatcaat tttctgttcc tgggtggatt
     4621 gaattactga gttaattaaa aaaaagcttg cattgagaat tgttctcagt tatgcttttt
     4681 gataagatcg cactgttatc tgaataacgg tgcatttttg tcagcttaag ataacagtgc
     4741 gatcttattt tttggaggtg aaagagtgtc aaaaatcaat ccgttaacct tgattacttt
     4801 aaatattttt tttccggtca ttattttttt aggaaaggga aatatatttc aagtggggtg
     4861 tttcattatt gctacagttt tagtattgat gtttcgtaaa ataaagacat gtatttattt
     4921 catcttgatt tattcagttc tgtttttagt aatgaaaatt attgatgcta tcaagataga
     4981 gttttttact atattttttg ggacatcctt atatattgta tttcgaatgt tgccggtcgt
     5041 aatgatctca tggttgttgg ttagtgtcta tactagcaat gaattattat cctctttaga
     5101 aaaaatacat ttaccgaaaa aaataatgtt aagtattaca gtagtgatca gattctttcc
     5161 gacttatcgt tctgaaataa gaatgattaa agaatcttta aaaatgagaa acattcgctt
     5221 gtcttttttt cagcctctgc aatatttgga atttctgtta gttcccgttc ttatacgtgc
     5281 aactatgatt gctgaagaaa tgaccgctaa tgcaataaca aaggggatag aatctcctgt
     5341 gaaaagaagt tcttactata aaacaaacat gagcttatta gactatatca tcctgttttc
     5401 cgttactatt tttttcttaa gtattttgat ttgaggtgaa agaattgttt gaatttaaca
     5461 atgtttcttt ttcatataag gatgttaagg aacaagcgct tgttgacctt aatttttcaa
     5521 taaaaaaagg cagtcttaca ttgattactg gttcttctgg gagtgggaaa tccacactat
     5581 taaagttaat gaatggactt attccaagac tttatgacgg aaaattggtt ggggaaattt
     5641 tatttgaaaa taactcgcta caatccttaa cacaagaaag tatatcaaaa aatattggct
     5701 acgtaagtca agatcctaga ggacaattct ttacaacaaa tacaacgagt gagttagtat
     5761 tttcgatgga aaactttgga tttacaaaag agcatatgga taaaagaatc aatgaattag
     5821 ctgaattatt agatctttca aatatattag ataaaaacat tttttcgcta tcaagtggtg
     5881 aacggcagaa aatttctatt ggatgtagtt tatcgctaaa ctctaaatta attatattgg
     5941 atgaaccgtc ttcaaatttg gacttcaaag cgactaagaa acttgctgac ttactttgtg
     6001 atttaaaaaa taaaggattt actattgtta tagctgaaca tcgtttttac tatttgaaag
     6061 aattaattga tcaagttcta tttgttaaaa atagaactgt aaaaactaca actattcagt
     6121 tgttaaaaac tgaaaacaat aacgcattaa ggaaatttga catttttaat agttcgattg
     6181 aagttcccta tatcgaaaag gtaagtgcag atgtattaaa agttgaagaa attacttatg
     6241 aaaatattct aaaagacata aattttgatg tcaaagttgg agatgttatt ggtttggtag
     6301 ggaaaaacgg tgtggggaaa acaaccttac taagaatact catgaagatt attcgcccta
     6361 ccaaaggaaa gataatcgaa aataaaaaaa atttgtcatc gccgttttta gtaatgcaag
     6421 aaatggatta ccaatttttc acagaatcag tgaggagcga gttagccctg gggaatgaaa
     6481 tagttagtaa agatcagcaa gaaaaaattt taaaaaaaat ggaactttat aaaatgaaag
     6541 atcaattacc gtttgattta tccggtggag aaaaacaaag attattggtt tctatagctt
     6601 cattgtcaaa aactaattta tttctatttg atgaacctac aagtgggttg gattatatca
     6661 atatggaaag gattgctgac cttattaaag acttaaaaga aaagggtgca gtagttatag
     6721 ccactcatga tccggaattt ctttataaaa catgcaatag aattatttat ttaaaaaatg
     6781 gtggaataaa aaaagatata aaacttagag tagaagattc aactttgata gaacagattt
     6841 ttaatgacat aacgaaatag aattgaggag aatatatatg aaaacaaaag attatatttt
     6901 tattggttta gctacagtta tttccctagt aatttacttt ttttcaataa tggttagtag
     6961 tattggtggg gcgtttggtc acagcatatc cccagggatt tttggattat tatctggaat
     7021 catatttgtt tatattagct ataactaccc tagaaaagga atttttacta tttacacaat
     7081 tgttttattg ttatttttta cgttaatggg cggggcgtac ttaccttggt ttatcagttc
     7141 gattagtatg gcgatcttag ctgatattat tctatctgta tttggatatg atcgagcaat
     7201 tcctcaagtt gtgtcttggg ttctaatgca actaggcagt gctgccggac agtggattcc
     7261 catatggttc tttacagatc gttttcgtca agactggata gatagaggac aatctgctgc
     7321 aacaatggat gcaatgatcc actacgcggt gggtatatgg ggaattattt cagtgttagt
     7381 tgtagcaagt ttatctatga ttggtgtatt gattggaaga aaagtcttaa aaaaatacaa
     7441 aaaatagaac gtagtttacc ttctatctgt ttgtacatct ctaatttacg ctattacgga
     7501 aagcattaac agttgtaaac gaataagaga agaaattgaa tgcgggggag gaaaaaatga
     7561 aagtatacaa aaaattattt agttatgtcc ctaaaatgaa atactacgga tggttagcca
     7621 ctttattttc tgatttttct gtcgtattaa cagtctatgg atattacagt atttacaaat
     7681 ttcttcagtc tctaattatt gtaggagatg aatatttagc taagagttat gcggttcaaa
     7741 ctgttgcttg gttaacttta ggagctttat tttatatttt ttctggctta ttttctcata
     7801 tccttgggtt tcgcttagag actaatttga gaaaaaaggg aattagcggt ttaacagaag
     7861 caagttttcg tttttttgat ttaaattctt cgggatatat tcgaaagaca attgatgaca
     7921 acgcggctaa aacacatatg gcaattgctc acttgattcc agatagtgct caagccatag
     7981 taacacctgt tttatcagtt gccttaggct ttttcattaa ctttaaaatt ggattggttc
     8041 taatcgccat gttagtaatt ggtgtattct tgttaaagaa aatgacagag aatacaaatt
     8101 ttattcaaaa atatcaagaa tcgttagatg tgttaagctc tgaaacggta gaatatatcc
     8161 gcggaattca agttataaag atatttggtg ctaaagtaac atcatttaaa gccttgaata
     8221 aagcaattaa cgattatgct aagtttgcat ataagtattc tttgagtggg aaaacacctt
     8281 ttgttctttt tcaatggcta ttttttggca tagtttgcat tttgatagtt cctataacct
     8341 tttttataac ttctcttacc actcccaaga tattagctgt tgacttgttg atgttgtttt
     8401 ttttaagtgg aattattttt gtttctttca tgagaataat gtatgtaagt atgcacttgt
     8461 ttgaagcaag atatgcagtc gaaacactag actcccttta tttagaaatg aagcaagacc
     8521 aactaaaata tgggaacgag acacagatta aaaattgtga cattgcattt gaaaatgtca
     8581 gcttttcata caaagaagag tgtgttttag aaaatttcaa cttagtttta gaacaaaaaa
     8641 gaatttatgc attagtagga aaatcaggaa gcggaaagag tacgatagct aaactaattt
     8701 caggtttcta caatgtggat aagggacaga ttaaaattgg tggtaagcct ttaaatcact
     8761 attcaaaaga cacaattatt aacacaattg cttttgtctt tcaagatacg aaactattca
     8821 atcagagtat ttacgataac gtttctatag caaatcctca agcgccagca agtaaagtat
     8881 ttgacgcgct aagattagcc ggttgcgaat cgattttaga aaaatttccc gaaagagagc
     8941 ataccttaat tggtagtaaa ggagtttatc tgtcaggagg agaaaaacaa cgtatagcta
     9001 ttgccagagc aattttaaaa gatgcaaaga ttgttattat ggatgaagcc agtgcatcta
     9061 ttgatccaga taatgagtat gaactacaaa aggcttttca atatttgatg aaagaaaaaa
     9121 cagttattat gatagctcat cgattatcca gcattaaatc tgtagatgaa attcttgtct
     9181 tgtcagaagg aaaaatagta gaacgtggaa atcataatga attaatggct aaacatcgag
     9241 tgtataaaca attggtcact aattatgaga atgcaaatga ttggaggctt gacaatgaaa
     9301 aatttttata aaaaaaaatt tgctttaact gatcagggcg cagaagcttt aactaaagct
     9361 tctatttcta gtttttttgt atactgcatt aatatggtac cagcattcat tataatgatg
     9421 ctgatagacg aattagtttt agaaaatgca aagccccgtt ggctttattt tgcagtatcg
     9481 tttgttactt tgcttttcat gtattggttg ttagataggg agtatgaaaa cttatataat
     9541 agtacttata aagaaagtgc gcatttaaga gtgcaaattg cagacgattt gtcaaattta
     9601 ccattatcct atttttcaaa acataattta tcagatttat ctcaaactat catgtctgac
     9661 gttgaaggta ttgagcatgc gatgagtcat gcaataccta aagccggcgg tatggctctg
     9721 tttttccctt ttatttcagt gatgcttttg gttggtaatg tcacaatggg attagctgtt
     9781 attttgccaa cgttatttag ttttgtctta atcttgttat caaagaaatc ccaaacgaaa
     9841 gccaatacta aatattacga tactttgaga gaaaactcgg aagaatttca agaaactatt
     9901 gaattgcagc aagagattaa tagctttaat ctatctaaaa aagttcaaga cagacttttc
     9961 aaaaaaatgg aagagagtga aaggattcat ttaaaggtag aattaagtac tttttcagtc
    10021 atggccttat cctctatttt ctcatatgtt agtttagcag tagtcattct agtaggtgtt
    10081 cacttactgt taacgggtga agtgactata ctctacgtcg ttggttactt actagccgca
    10141 ataaaaataa aggattcctt cgattctatg aaagaagcag tgcttgaaat attttattta
    10201 gctcctaaaa tacagcgaat tagagctatg aaggaaacca gtatccaaga gggatcagat
    10261 agtcctctga aatcttttga cattgagttg cgagatgtct ctttctcata tgataacaat
    10321 accccaattt tagatcatat ctcttttacg gctaaacaag gagaagtgac tgcgttagta
    10381 ggtgctagtg gttccgggaa aactagtatc ttgaaattag tgtctagact gtatgattat
    10441 gatgaaggct gtattttaat tgatgggtat gatattaaac gtgtatctcc agcttctttg
    10501 tttagcaaaa tcgctattgt ttttcaagag gtaactttgt ttaatacaag tattttggaa
    10561 aatatacgta ttggaaatag tcaagcgtca gatgaggaag tgaaaaaagc agctcgttta
    10621 gctaactgcg aagactttat tgaaaaacta cctgacggtt atcatacatt ggtcggagaa
    10681 aacggaagtt cactttctgg aggagagcga caacgcctat ccatagcacg ggcttttttg
    10741 aaaaatgcgc caattttaat tttagatgaa attactgcca gtttagatgc ggaaaatgaa
    10801 aaacgtattc aggaaagttt aaatcgttta atacaagata agacagtact aattatatcg
    10861 catcgcttga agtctattga aaaggtaaat aaaattgtag tgatggatca aggaaaacta
    10921 gttgatcaag gtactcataa tgagttgtat cgaaggtcag aaatatacaa aaatctcata
    10981 aagaaaacta aactatctga gaaattcgtc tatgaaaaag aagctcaatc acgatgatga
    11041 aataggcttt aaattaacct acaatttttg ggatatagtt ataattcttt ttcaaaaaga
    11101 ataattttat taaacagcat ctaattttga atacacgaaa ga
//
LOCUS       DACEZN010000088        10944 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEZN010000088 DACEZN010000000
VERSION     DACEZN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.088"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(192..1448)
                     /locus_tag="IXH60_001829"
     CDS             complement(192..1448)
                     /locus_tag="IXH60_001829"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1990741.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            2028..3329
                     /locus_tag="IXH60_001830"
     CDS             2028..3329
                     /locus_tag="IXH60_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1990742.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3398..3997)
                     /locus_tag="IXH60_001831"
     CDS             complement(3398..3997)
                     /locus_tag="IXH60_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1990743.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            4251..5132
                     /locus_tag="IXH60_001832"
     CDS             4251..5132
                     /locus_tag="IXH60_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990744.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            5145..6755
                     /locus_tag="IXH60_001833"
     CDS             5145..6755
                     /locus_tag="IXH60_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1990745.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(6832..7491)
                     /locus_tag="IXH60_001834"
     CDS             complement(6832..7491)
                     /locus_tag="IXH60_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1990746.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(7774..8439)
                     /locus_tag="IXH60_001835"
     CDS             complement(7774..8439)
                     /locus_tag="IXH60_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990747.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(8586..9191)
                     /gene="dhaL"
                     /locus_tag="IXH60_001836"
     CDS             complement(8586..9191)
                     /gene="dhaL"
                     /locus_tag="IXH60_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1990748.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(9219..10202)
                     /gene="dhaK"
                     /locus_tag="IXH60_001837"
     CDS             complement(9219..10202)
                     /gene="dhaK"
                     /locus_tag="IXH60_001837"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1990749.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(10204..10599)
                     /gene="dhaM"
                     /locus_tag="IXH60_001838"
     CDS             complement(10204..10599)
                     /gene="dhaM"
                     /locus_tag="IXH60_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1990750.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(10689..>10944)
                     /locus_tag="IXH60_001839"
     CDS             complement(10689..>10944)
                     /locus_tag="IXH60_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1990751.1"
                     /translation="NTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNKVSE
                     TFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
BASE COUNT         3056 a         2247 c         1977 g         3664 t
ORIGIN      
        1 attttaatat gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga
       61 gggaatttac aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat
      121 tttaggatgg gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa
      181 cttactaaat tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata
      241 accacaaact taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg
      301 cggtcaccgt taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat
      361 ggttcaattt ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca
      421 acgttcggca ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt
      481 ccagagaaca atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca
      541 aatcttgtaa cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct
      601 acttttttcg ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat
      661 ttcactacat cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt
      721 ttcttcggat caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc
      781 ttcagcatca atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg
      841 attaggtcta atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc
      901 acatcgtatg ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac
      961 tctgtaaatg agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg
     1021 ttgatgttga agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac
     1081 cagtcgtagt tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga
     1141 ttggaaagtg cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt
     1201 ttgcggccgc ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga
     1261 tggcctgcca attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc
     1321 gaatcaccag ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg
     1381 cgaagtcttt cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata
     1441 atattcattt tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatgt
     1501 gtaattaatt atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc
     1561 caagggacga aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct
     1621 cgaacattta acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct
     1681 ttaaagcatg tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact
     1741 acttcgtttc gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt
     1801 ttctttgtca acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc
     1861 cgctctaatt ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat
     1921 tttttctgca atttatcgag aagtcacttt actttgcctc ttattctttc ttcgttacaa
     1981 taaggaatgg gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc
     2041 aaaaaattat tttaggagcc acttctgctt taactatcgt tattttaatt ctaagtttgt
     2101 tttttgtcat ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag
     2161 aaaaagcaaa agaaccgaca gatgataagc aacaactagt tactcaagca aaaaatctgg
     2221 ctcaaagcta cgattatgac caagcgattg cgttattgga aaaagatagt aagaacggat
     2281 ctactgaaac acaacagctt cttcaaacac taaaaaagga gaaagaacaa ctgatcgact
     2341 ggtctgatcc tactcagatc tctcatgtct ttttccacag cttgatcgtg gacccgcaaa
     2401 aagccttcca ctctcaacag gcacaaggat acaaagatta tatggtgact gttgaagaat
     2461 tcaaccgttc gatcgaacaa ctttatcaaa atgattttgt tttagttaat ttgaatgatt
     2521 tgatccaaaa agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa
     2581 aaaaaccatt ggttctttca caagatgatg tcagctatta tgaatatatg gataattccg
     2641 gttttcctag ccgattggtc gtatcaaata acaatgaaat caaaaatgtc tataaagaga
     2701 aagataaaga agaaatcggt gactttgaca tggttcctct gatcgatgcc tttgtcaaga
     2761 aacatcctga tttttcttat cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg
     2821 gagttttagg ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag
     2881 agattaaaga agctaaaaaa gtggcggaac agttgaagaa agacggctgg agctttgctt
     2941 cccatacatg gggtcacttg aatatgactc aagcttcact agaagatgta aaaaaagaca
     3001 atgaattgtg gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt ctgatttatc
     3061 catttggtgc agacatcagc gactggcagc cttattcagc agaaaatcaa aaatttgctt
     3121 acttgaagag tcaaggtttc gatattttct gtaatgtcga tgcttctaca ccagcttggg
     3181 gacaattagg tacggatttc tatcgaaatg ctcggatcaa tatcgatggc attcgttttc
     3241 aatctgattt agacggaaaa aatcctattc ttgatccatt catcaatgtc catgatgtct
     3301 atgacaaaga agcacgcgaa tctgcttgat tttacgtcat ttatgaacca aaaaagccga
     3361 actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc ggtgcgattc
     3421 ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg tatatctccg
     3481 gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca tccgaatatt
     3541 tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg atactgaaac
     3601 tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt tttgtatagt
     3661 atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg attttgtttt
     3721 cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac gctttcgctt
     3781 cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct tccttgctgc
     3841 caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc ttatccattg
     3901 tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt
     3961 tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc cgtaatttta
     4021 aacttttata atatatatag ttcaaattta ttctgaggtt tattataact atctattgag
     4081 aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt ataaaaagaa
     4141 tattatgtac taaaataata aaaaaagttt tataaaaagg tataaaacct ttttattaaa
     4201 ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa
     4261 tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct
     4321 ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat
     4381 ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt
     4441 ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag
     4501 gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc
     4561 ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc
     4621 ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg
     4681 ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt
     4741 tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc
     4801 tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac
     4861 gcgaaggaac actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga
     4921 ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg
     4981 actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga
     5041 ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg
     5101 aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt
     5161 cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat
     5221 ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt
     5281 ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg
     5341 tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg
     5401 aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc
     5461 aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac
     5521 gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa
     5581 tatcgatcag ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt
     5641 ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg
     5701 ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg
     5761 gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc
     5821 ctttaggctg gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt
     5881 atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag
     5941 atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt
     6001 tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca
     6061 cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag
     6121 aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact
     6181 ttacaagcac gatcatggtc gtcttgattt gtctagctgc tattttacca attgctagca
     6241 tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa
     6301 tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt gctatattgg
     6361 caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga
     6421 aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag
     6481 tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt
     6541 atatctactt agcttactcc tttgcactca attactttgg cggaattttg gatttaccag
     6601 aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg
     6661 atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta
     6721 ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc
     6781 tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc
     6841 ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt
     6901 ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc
     6961 aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc
     7021 aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg
     7081 ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata
     7141 atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag
     7201 tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc
     7261 gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt
     7321 catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc
     7381 tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac
     7441 taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc
     7501 tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca actgataatt
     7561 ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct
     7621 ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt
     7681 ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa
     7741 ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt
     7801 ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat
     7861 ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt
     7921 caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag
     7981 ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc
     8041 tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt
     8101 ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca
     8161 ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt
     8221 ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag
     8281 tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt
     8341 gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg
     8401 tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat
     8461 cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc
     8521 tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat
     8581 tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca
     8641 gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta
     8701 gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct
     8761 gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct
     8821 gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct
     8881 tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg
     8941 tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat
     9001 gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct
     9061 cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat
     9121 tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg
     9181 gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca
     9241 ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga
     9301 ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct
     9361 ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc
     9421 ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa
     9481 atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag
     9541 cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct
     9601 ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc
     9661 tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca
     9721 tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac
     9781 gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc
     9841 atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg
     9901 atcagcaaaa ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga
     9961 gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac
    10021 ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct
    10081 ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt
    10141 tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc
    10201 aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc
    10261 atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac
    10321 cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa
    10381 taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac
    10441 tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa
    10501 aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac
    10561 tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac
    10621 atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt
    10681 ttcgtttgtc aaattaagct agacaaatca tctttaccta cgtaactcaa aaatacttcc
    10741 aatggtgtgc gatagtgtaa tgattttcta ggaatatgat ttcgttttga tgccaccgat
    10801 tggataaagg tttcggatac cttattaaaa tccatagctt tatataagcc atctttacgt
    10861 aataagccat tcgaatgttc atttagtcct cgttgagaag gtgttcctgg atcagcgaaa
    10921 taaatcgaaa tatcattggt atta
//
LOCUS       DACEZN010000089        10928 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEZN010000089 DACEZN010000000
VERSION     DACEZN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.089"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..381)
                     /locus_tag="IXH60_001840"
     CDS             complement(<1..381)
                     /locus_tag="IXH60_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1990752.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLY"
     gene            487..956
                     /locus_tag="IXH60_001841"
                     /pseudo
     CDS             487..956
                     /locus_tag="IXH60_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1062..1646
                     /locus_tag="IXH60_001842"
     CDS             1062..1646
                     /locus_tag="IXH60_001842"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1990753.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            1649..2722
                     /gene="prfA"
                     /locus_tag="IXH60_001843"
     CDS             1649..2722
                     /gene="prfA"
                     /locus_tag="IXH60_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1990754.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            2715..3554
                     /gene="prmC"
                     /locus_tag="IXH60_001844"
     CDS             2715..3554
                     /gene="prmC"
                     /locus_tag="IXH60_001844"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1990755.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            3547..3726
                     /locus_tag="IXH60_001845"
     CDS             3547..3726
                     /locus_tag="IXH60_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990756.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            3748..4773
                     /locus_tag="IXH60_001846"
     CDS             3748..4773
                     /locus_tag="IXH60_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1990757.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            4948..6192
                     /locus_tag="IXH60_001847"
     CDS             4948..6192
                     /locus_tag="IXH60_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1990758.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            6345..7292
                     /locus_tag="IXH60_001848"
     CDS             6345..7292
                     /locus_tag="IXH60_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1990759.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            7426..8055
                     /gene="upp"
                     /locus_tag="IXH60_001849"
     CDS             7426..8055
                     /gene="upp"
                     /locus_tag="IXH60_001849"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1990760.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(8194..8436)
                     /locus_tag="IXH60_001850"
     CDS             complement(8194..8436)
                     /locus_tag="IXH60_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990761.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            8557..8629
                     /locus_tag="IXH60_001851"
     tRNA            8557..8629
                     /locus_tag="IXH60_001851"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8590..8592,aa:Thr,seq:cgt)
     gene            complement(8748..9386)
                     /locus_tag="IXH60_001852"
     CDS             complement(8748..9386)
                     /locus_tag="IXH60_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1990762.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(9509..10141)
                     /locus_tag="IXH60_001853"
     CDS             complement(9509..10141)
                     /locus_tag="IXH60_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1990763.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            10492..10791
                     /locus_tag="IXH60_001854"
     CDS             10492..10791
                     /locus_tag="IXH60_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1990764.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         3777 a         1816 c         2244 g         3091 t
ORIGIN      
        1 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
       61 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
      121 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
      181 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
      241 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
      301 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
      361 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
      421 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
      481 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
      541 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
      601 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
      661 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
      721 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
      781 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
      841 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
      901 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
      961 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     1021 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     1081 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     1141 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     1201 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     1261 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     1321 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     1381 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     1441 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     1501 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     1561 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     1621 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     1681 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     1741 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     1801 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     1861 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     1921 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     1981 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     2041 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     2101 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     2161 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     2221 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     2281 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     2341 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     2401 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     2461 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     2521 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     2581 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     2641 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     2701 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     2761 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     2821 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     2881 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
     2941 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
     3001 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
     3061 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
     3121 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
     3181 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
     3241 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
     3301 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
     3361 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
     3421 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
     3481 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
     3541 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
     3601 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
     3661 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
     3721 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
     3781 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
     3841 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
     3901 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
     3961 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
     4021 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
     4081 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
     4141 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
     4201 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
     4261 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
     4321 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
     4381 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
     4441 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
     4501 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
     4561 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
     4621 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
     4681 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
     4741 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
     4801 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
     4861 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
     4921 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
     4981 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
     5041 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
     5101 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
     5161 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
     5221 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
     5281 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
     5341 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
     5401 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
     5461 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
     5521 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
     5581 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
     5641 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
     5701 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
     5761 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
     5821 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
     5881 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
     5941 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
     6001 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
     6061 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
     6121 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
     6181 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
     6241 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
     6301 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
     6361 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
     6421 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
     6481 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
     6541 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
     6601 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
     6661 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
     6721 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
     6781 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
     6841 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
     6901 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
     6961 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
     7021 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
     7081 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
     7141 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
     7201 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
     7261 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
     7321 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
     7381 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
     7441 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
     7501 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
     7561 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
     7621 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
     7681 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
     7741 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
     7801 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
     7861 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
     7921 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
     7981 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
     8041 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
     8101 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
     8161 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
     8221 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
     8281 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
     8341 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
     8401 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
     8461 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
     8521 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
     8581 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
     8641 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
     8701 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
     8761 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
     8821 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
     8881 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
     8941 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
     9001 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
     9061 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
     9121 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
     9181 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
     9241 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
     9301 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
     9361 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
     9421 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
     9481 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
     9541 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
     9601 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
     9661 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
     9721 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
     9781 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
     9841 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
     9901 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
     9961 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    10021 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    10081 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    10141 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    10201 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    10261 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    10321 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    10381 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    10441 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    10501 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    10561 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    10621 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    10681 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    10741 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    10801 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    10861 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    10921 ttttatcc
//
LOCUS       DACEZN010000090        10575 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEZN010000090 DACEZN010000000
VERSION     DACEZN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.090"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(44..682)
                     /gene="plsY"
                     /locus_tag="IXH60_001855"
     CDS             complement(44..682)
                     /gene="plsY"
                     /locus_tag="IXH60_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1990765.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            809..1243
                     /locus_tag="IXH60_001856"
     CDS             809..1243
                     /locus_tag="IXH60_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1990766.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1409..3454
                     /gene="parE"
                     /locus_tag="IXH60_001857"
     CDS             1409..3454
                     /gene="parE"
                     /locus_tag="IXH60_001857"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1990767.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3466..5916
                     /gene="parC"
                     /locus_tag="IXH60_001858"
     CDS             3466..5916
                     /gene="parC"
                     /locus_tag="IXH60_001858"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1990768.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6193..8424
                     /gene="pflB"
                     /locus_tag="IXH60_001859"
     CDS             6193..8424
                     /gene="pflB"
                     /locus_tag="IXH60_001859"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1990769.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8668..9429
                     /gene="pflA"
                     /locus_tag="IXH60_001860"
     CDS             8668..9429
                     /gene="pflA"
                     /locus_tag="IXH60_001860"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1990770.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9514..10440
                     /locus_tag="IXH60_001861"
     CDS             9514..10440
                     /locus_tag="IXH60_001861"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1990771.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
BASE COUNT         3741 a         1833 c         2267 g         2734 t
ORIGIN      
        1 gatttgtcac actcccgttt tatctctttg gaatgaaaaa gtatcaggat tttttcttgc
       61 tgctattcaa accaaacggg attcgacttt cagtatggtt tctgatacgc ttgatattgt
      121 ctttatgccg ataaaagata aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat
      181 tgggcttagc caaaatcaca ggagctacat aaggaaggac gatcgtagat aaagtgatca
      241 atactgcact gatcatactg ctcaaactca ccatacttgt acaaaataaa ctaacgataa
      301 agatgataca agaataaaca aaaaatacag gattaaatcc taaaagcatt cctgcacttg
      361 tcgcaacagc ttttcctcct ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg
      421 cgcatacccc aaaccaaaga gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag
      481 tagccaatgt accttttaaa atatccataa gcaggacaac aatcccggct gttttgccta
      541 atacacgaaa tgtattggtc gttccagtat taccgctccc gtgttgtcgg atatcttttt
      601 tgaaaaacaa tttgccaatc cagacaccgg aaggaataga acctaaaaga tatgctaaaa
      661 taagtaataa gatcaatttc attttttacc ctctttcaag ttgaaaacta tcctttattt
      721 tagcatgaat aggtaacagg aacaattgaa aaaatgaaag atacaagtat aatagatgga
      781 ataaaaagaa aagcaagggg tggcagaaat gtctgtaaaa aatccaacac aagaaacgat
      841 tttcaactat ttggaagaag caaaaaatat tgccgtcgta ggcttgagca ataaaccaga
      901 acgaacaagt tacatggttg cacaggtatt gcaagacaat ggatataaaa tcataccagt
      961 aaatccatta ttggctggtg aagaaattct aggcgagaaa gtctatgcga atttgacaga
     1021 aatcgatgat ccaatcgata tcgtagatgt atttagacgg agtgaatttt tacctgaaat
     1081 agcaggtgat tttttgaaaa ctgatgctaa agtgttctgg gcacaattag ggattgaaaa
     1141 caaagaagca gctgagatgc taagaaaagc aggacgtaca gatatcgtaa tggatcgttg
     1201 tattaagatt gaacttggta agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag
     1261 agatgatcga tagaggggaa tgtcgaaaaa gcggttagga gataattaaa attgatttct
     1321 cgtctaccct cttttttttc acgacctttt ctagtatgat aatcgtggtg cggttttgcc
     1381 gcaaagtatt attgaaggag cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga
     1441 ctcgtccatc caagtattgg aaggattaga ggcagttaga aaaagaccag ggatgtacat
     1501 tggatccact gacagccgag gcttgcatca tttagtctat gaaatcgtcg ataatgcagt
     1561 tgacgaagca ttatcaggtt atggcagtga gattgatgtg accatccatg aagacaatag
     1621 tataactgtt gcagatagcg gacgtgggat gcctgtagga atgcatgctt ccggaatacc
     1681 aacagttgaa gtgattttta ctgtgctgca tgctggcgga aagtttggtc aaggagggta
     1741 caaaacttct ggtggactgc acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg
     1801 gctaattgta acgatcgtta gagatggcgt agagtatcag cagaaattta aaaacggcgg
     1861 taaacctgac ggtactttga aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt
     1921 ccatttttta ccagatgata caatattcag tacaacgaaa ttttcatatg aaattttagc
     1981 cgaacggtta agagaatctg cctttcttct aaaaggcgtt aaaatctctc tttctgatct
     2041 tcgtggagaa gaaccagtca aggaaatttt tcattacgaa gaaggaatca aagaatttgt
     2101 tgattatctg aatgaagaaa aagatacatt gacaccagtt gtttattttt caggagaaaa
     2161 agaaggtatc gaagtagagg tcgcttatca atacaatgac ggttattcag aaaatgtttt
     2221 atcttttgtc aataacgttc ggacaaaaga tgggggaacc catgaagctg gtatgaaagc
     2281 ggcgatgacg aaatcttata atgaatatgc gagaaaagtt ggtttgctga aagaacggga
     2341 caagaatctg gaaggtagtg atttcagaga agggttagct gctgtacttt ctatccgtgt
     2401 acctgaaaat cttctgcaat ttgaagggca aacaaaagaa aaacttggta cgccagttgc
     2461 tcgtaccgta gtagataacg tcatcagtga acaaatgggc ttttatttgc aggaaaatag
     2521 cgaaatgagc cagatgttgg tccgtaaagc aatcaaagcc agagaagcga gagaagcagc
     2581 acgtaaagcc agagaagaaa gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct
     2641 ttcaggaaaa ttgacacccg cacaatcccg aaaccctaag aaaaatgaat tgtatttagt
     2701 cgaaggggat tctgctggcg gatctgctaa acaaggaaga gacagaaagt tccaagcaat
     2761 tctaccttta cgaggaaaag tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa
     2821 aaatgaagaa atcaatacga tgatctatac gattggtgca ggagtcggtc ctgagttttc
     2881 tatcgaagac tgtaattacg acaaagtaat catcatgacc gatgcggata cagacggcgc
     2941 ccatattcaa gtattactgc ttactttctt ttatcgttat atgaaaccgc tgattgaagc
     3001 tgggaaagta tatatcgcgt taccgccttt atataaagta tccaaaggac aaggaaagaa
     3061 gcaagtcatt gaatatgcgt ggacagatga cgaattggca gcaatgatta aaaaagtcgg
     3121 caaaggatat atgctgcagc gatataaagg acttggagag atgaatgcgg aacaattgtg
     3181 ggaaacaacg atggatccga cttcccgtac gttgatccgc gtccgtatcg atgatgctgc
     3241 gcaagctgaa cgtcgagtaa ccacactgat gggtgacaaa gtagaacctc gaagaaaatg
     3301 gatcgaaaat cacgtgcaat ttacgctcga agaagatggc agtatcttag ataaaaaaga
     3361 ggatactgaa atctctcctt cagtttctaa tgatttatta gatgaagaac gagcagataa
     3421 aaatgaaaac aatcagttat ttgaagtaga ataggaggtc acctgatgga aaatcgacaa
     3481 gaagtacaag aattaacttt agaagaagtg atgggtgacc gcttcggacg ttattccaaa
     3541 tatattatcc aagaacgtgc actgcctgat atccgtgatg gattgaagcc agtgcaacgc
     3601 cggatattat ttgccatgaa caaagatggc aatacgtatg acaaaggatt ccgtaaatca
     3661 gcaaaatctg tcggaaatat catggggaat tatcatcctc atggtgaccg cagtatttat
     3721 gaagcgatgg tccgcatgag ccaagactgg aagttgcggg aagtactgat cgaaatgcac
     3781 gggaataacg ggagtatgga cggtgatcca cctgctgcga tgcgttatac tgaagccaga
     3841 ttatccaagt taagtggaga gatgctagca gatatcgaga aagaaacagt cgatctcgtg
     3901 tggaattttg atgatacaga aaaagaacca acagtccttc cagctcgata cccaaacctt
     3961 ctcgtcaatg gatcgacagg tatatctgct ggatatgcaa cagaaatccc gactcataac
     4021 ttagctgaag tgatcgatgg cacaatctat atgatcgatc atccacaagc gagccttgaa
     4081 aaattgatgg aatatattcc tggacctgac tttccaacag gagggatctt gcaaggaaaa
     4141 gatgaaatca aaaaagctta tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca
     4201 aagatagagg caatgaaggg taataaacag caaatcgtga tcacagaaat cccatatgaa
     4261 gtaaataaag cgaccttggt gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat
     4321 ggaatagctg aagtacgaga tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg
     4381 aaaaaagacg ccaatgctca aggaattttg aattatttgt tcaaaaacac tgagctacag
     4441 atcaattaca atttcaatat ggtcgctatc gaccatatga ctccgcatca agttggactg
     4501 aaagacattt taagaagtta tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa
     4561 ttcgatttag ccaaagctca aaaaagacag catatcgtag aaggattgat gaaagcttta
     4621 tcgattctgg atgaagtaat cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa
     4681 aagaatttag tcgacgtctt tcaatttaca gaagaacaag cagaagcaat tgtcacattg
     4741 caattatacc gtttgacgaa cacggatatc acagagcttc aaaaggaatc cgaaagcttg
     4801 attgcgcaaa tcacagagtt gaataaaatc ctttcaaatg acaaagagct tttctctgtt
     4861 atgaaaaaag aattgcgaga agtgaaaaaa aattattctt ccgctcgtct aacgacaatc
     4921 gaagacgaaa tcgaagaaat caaaattgat acgcaagtct tagtggcaca agaagacgta
     4981 attgtcagtg tgacgcgaga aggctatgtg aagcgaacaa gccttcgttc ttatagtgct
     5041 tccaaaccag aagaaatcgg gatgcgagaa ggcgactact tgctatactc aggtgaattg
     5101 agtacattgg accatgttct tctgatcaca aataaagcaa atgtgatcta ccgccctgtc
     5161 catgaactac ccgatttgaa atggaaagat gctggtgagc acatttctca aacaatctcc
     5221 aatctgtctg tagacgaatc gattttagct gttttcccat atcaaaaaat tcaagcagag
     5281 aaaacatttg tattcatcag caagaatgga ttgatcaagc aaacacgaat gacagagttc
     5341 gaaccatggc gcacctataa gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa
     5401 gatgaactga ttgctgtgta tctgaaagaa gatcagccag gcttagatgt tttcttagta
     5461 acgcatcaag gaatgggttt gcgctatcct ttaacagatg ttcccgttgt tggagcaaag
     5521 gcagctggag tgaagtctat gaatttgaaa gatggagata ggattatcaa cgggctgctt
     5581 gtgttagcag aaggtgatac gccgatcgta atcgttactc agcgaggagc tgttaaacgc
     5641 atgcttgctc aagaaatcag ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt
     5701 gagctgaaaa aaaatccgca ccgtattatc tatatgtcgg aaggaagatc aaaagaaata
     5761 accttgatga atcaaaaagg gcagcagtta acgattgacc caactgattt ccctataggt
     5821 gatcgaacat cgaatggatc atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa
     5881 gttatcgatg ctcctgagat tttcatcgag tcgtaactgt tgtagtattt taatgaagag
     5941 acgccatctg tattaataca ggtggcgtct ctttttcatt catttttaat aaatccttat
     6001 acgtttgcaa aaaaagatga aagtgttcac aatgcttaaa tgttggtatg tcaacgttga
     6061 tttaaatata tgtaaataaa acataaaaat tgttaaataa aaaacaagaa aacccttgca
     6121 aagatagggt gctgtgttat actaatcacg taaaaactgt tacataaaac agaaatttag
     6181 gaggtccata caatggagca atggaatgga tttaaaggta ccaaatggaa agaaggcgta
     6241 gacactagag actttattca aaataactac actgaatatc gcggtgatga cagctttttg
     6301 gaaggcccaa cagaagcaac agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc
     6361 caattcgagc gtaacggtgt ctatgatatg gacacagaaa tcgtatcaac tattacttca
     6421 cacgatgcag gctatttgga taaagatttg gaaaaaatcg tcggtctaca gacagacaaa
     6481 cctttgaaac aagcattcat gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa
     6541 agcaatggct atacaccaaa cgatgaattg acacatattt tcactgactg gcgcaaaaca
     6601 cataatcaag gtgtatttga cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa
     6661 atcattacag ggcttccaga cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt
     6721 gtggcattgt atggaatcga ctatttgatg gaacaaaaga aaaaagatca tgatatgact
     6781 ggttatcata caatgagtga agaagtgatt cgtttacgtg aagaaatcac tgaacaatat
     6841 cgtgcattag cgcaaatgaa agaaatggct gcgaaatatg gctatgatat ttctaaacca
     6901 gctgcaaatg caaaagaagc tgtccaatgg ctatacttcg gatatttagc agcaatcaaa
     6961 tcacaaaatg gtgctgcgat gtcaatcgga cgtatctcag cattccttga tatctatatc
     7021 caacgtgatt tggataatgg agtaatcaca gaaaaagaag cacaagaatt gatcgaccac
     7081 ttagtaatga aattacgtat ggtcaaattc gcgcgtacac ctgaatataa ccaattattc
     7141 tcaggattcc ctatctgggc tactttatcc attgcaggta tgggattaga tggtcgttca
     7201 ttagttacaa agaatgattt ccgtctgctt catacattaa caaatatggg accttcacca
     7261 gaaccaaact tgactgtttt gtattctgaa caccttccag aaggattcag aacatatgct
     7321 agcagaatcg ctatcgaaag ttcttcgatt caattcgaaa atgataatca gcttcgtgaa
     7381 caatggggtt ctgatgattg tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa
     7441 gacatgcaat tctttggcgc tcgtgctaac ttggctaaaa cactattata tgccatcaat
     7501 ggcggagtcg atgaagtgac tggcgcccaa gttggaccag aatatcgtcc aatcacatca
     7561 gaaatactag actttgaaga agtgaaacat aacttcatga atatgatgga ttggttagca
     7621 gaactatatg tcaatacgtt gaacatcatt cattatatgc atgataaata ttcttatgaa
     7681 gcagctcagc tggctttgat ggattctgac ttgcaacgca catttgctac aggtattgct
     7741 gggatctctc atgcggcaga cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc
     7801 cgtaatgaaa aaggaatagc agttgatttt gaagtggatg gcgacttccc taaatacgga
     7861 aatgatgatg accgtgcaga tgaattagtt tcatggatct tggaatactt catgggacaa
     7921 atcaaacgtc ataaaactta ccgcaactca aaaccaacaa cttcattatt gacgattact
     7981 tcaaatgttg tttacggtaa aaatacagga aatactcctg atggccgtga agctggaaaa
     8041 ccacttgcgc caggagcaaa tccaagttac aatgcggaac aaagtgggtt attagctagc
     8101 ctgaactcaa cagctaaaat gaattacggc tgtgcattgg atggaatttc aaatacccaa
     8161 acaatcaatc cttcaggtct tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat
     8221 gtcttagatg gatacttctc aaaaggagct tatcatttga atgtcaatgt attcagtaat
     8281 gatttattgc gtgatgcaat ggaaaatcca atgaaatatc cgaacttgac gatccgggta
     8341 tctggttatg cagttaaatt ccgtgacttg acaagagaac aacaattaga tgtcttgatg
     8401 agaacttcac acgacagaat gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga
     8461 aaggacgttg aatagccatc gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa
     8521 cgatttacag gggcaggact tgtatgttcc gtccctgtaa attttacaca taacgaagcg
     8581 gcaattgcga aaaattttgt tcagaaagct tcgcttcgac agctaacttg tacttaaaat
     8641 gaaatcaaag aaaagatgtg aacttaaatg gaagaaaaaa caatcggcta tgttcattct
     8701 atcgaaactt tcggttcagt agacggacct ggactgcgct ttgtcgtgtt tatgcaagga
     8761 tgccgcatgc gttgccaatt ttgtcacaat ccagatactt ggaatattgg cggagggaaa
     8821 gaatacacag cagatgaact tttagataaa gcagaacgct tcagacctta ctggggagat
     8881 aaaggtggta ttactgttag tggtggagaa cctctattgc agatcgattt tttgatcgaa
     8941 ctatttaaaa aagcaaaaga gcggaagatg catactacat tggatacatg cggaaaacct
     9001 tttacgtatg aagaaccttt cttttcacgt tttcaagaat tgatgaagta tacagattta
     9061 ctgttatttg acatcaaaca catcgataat gaagagcata aaaaactaac gcattgggac
     9121 aatgaaaata ttttagaaat ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt
     9181 cgccatgtac tagttcctga gcgaagtgat tatgatgaat atttgatccg actagacaac
     9241 tttattcaaa ccttgtcaaa tgtagacaga gtagagattc ttccttatca tacgatggga
     9301 aaatacaagt gggaaacact tggtttgaaa tatccattag aagggatcga accacctaca
     9361 aaagaacgtg tcgaaaatgc taaacgattg cttcatacag aagagtatac aaaatattta
     9421 acgcgatagg tcgcatcgga aagaagataa gtattgcttc tttccgtttt tttgctatta
     9481 ttaaatagga gaaatcacaa aggagcgaat gacatgtcaa aaattttagt ttttggtcac
     9541 caaaatccag atacagatgc aatcggggca gccattgcgt tttcacactt gcaaaatgaa
     9601 ttaggtaaag aaacagaagc agttgctcta ggtacaccta atgaagaaac acaatacgca
     9661 ttagaccact ttcagctatc tgcaccacga gtagtagagt cagctaaagc agaaaatgca
     9721 gatgtcatgc ttgtggatca taatgaattc caacaaagta tttcagatat cagcgaagta
     9781 aatattttag ctgtagtaga ccaccatcgt attgcaaact ttgaaacagc taatccattg
     9841 tactatcgtg cagaaccagt tggatgtaca agtacgatta ttttaaaact atataaagaa
     9901 aaccaagtaa caattcctaa agaaatcgca ggaatcatgc tctcagctat catttcagat
     9961 acattgttgt tcaaatcacc aacatgtacg cctgaagatg aagcagctgc aaaagaactt
    10021 gcagcaatcg ctgaagtcga tctaaacact tatggacttg aaatgttgaa agcaggaaca
    10081 aatctaagtg ataaatcagc tgcggttctt cttgatttgg atgcaaaaag tttcccaatg
    10141 ggcagttcca acgttcgtat cgctcaagtc aatacagtgg accttaatga agtgatggat
    10201 cgacaagcag agctagaagc agcaatggaa gatgaaatga aagcaaatgg ctacgatctg
    10261 ttcgtattag ttgtcacaaa catcttagac agtgattcag aattacttgt tgctggtaaa
    10321 ggaaaagaaa aagtagaaga agctttccaa acaactttga ccaacaaccg tgctttgctt
    10381 aaaggtgtcg tttcacgtaa aaaacaagtc gtacctcaat tgacggctgt atttagctaa
    10441 tcaaatggta gtatgaagca ttcaaaatga atggataagc gattgatata aaaagaaaca
    10501 tagaacatac taaaggccga agaaatggag gagaataaca gtcaattatg actcttgttc
    10561 ttcctccttt ttacg
//
LOCUS       DACEZN010000091        10524 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEZN010000091 DACEZN010000000
VERSION     DACEZN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.091"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            155..2851
                     /locus_tag="IXH60_001862"
     CDS             155..2851
                     /locus_tag="IXH60_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1990772.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2928..3416
                     /locus_tag="IXH60_001863"
     CDS             2928..3416
                     /locus_tag="IXH60_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1990773.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            3428..3922
                     /locus_tag="IXH60_001864"
     CDS             3428..3922
                     /locus_tag="IXH60_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1990774.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3943..4752
                     /locus_tag="IXH60_001865"
     CDS             3943..4752
                     /locus_tag="IXH60_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1990775.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4749..5570
                     /locus_tag="IXH60_001866"
     CDS             4749..5570
                     /locus_tag="IXH60_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1990776.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5621..7720
                     /locus_tag="IXH60_001867"
     CDS             5621..7720
                     /locus_tag="IXH60_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1990777.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7717..8835
                     /locus_tag="IXH60_001868"
     CDS             7717..8835
                     /locus_tag="IXH60_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1990778.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8874..9752
                     /locus_tag="IXH60_001869"
     CDS             8874..9752
                     /locus_tag="IXH60_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1990779.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9898..10095
                     /locus_tag="IXH60_001870"
     CDS             9898..10095
                     /locus_tag="IXH60_001870"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990780.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         3251 a         1705 c         2322 g         3246 t
ORIGIN      
        1 aatctattaa taaaactaat atatgaatcc agttcaatat ttgtagctat gaaaaaacat
       61 aaaaatcata ttagataaaa actaaattgg tagataaatt attttgtcag atatctgagg
      121 caaatattcg actatttgtg tttcaaagta aaaattggaa tacaaatata gaatagacat
      181 attgcagccg atagtttatt atgcttttag acacattaaa ctggaggaaa aaatgaaaaa
      241 gagcgctaaa gatcgagttt ttgatgagct gatacgacta acagggaata tgtcttctgt
      301 cactgccgag aaaatttcga agaatctagg tatctctcgt cagaatgcca gccattattt
      361 gacgagatta gtggaagata aaaaggtcga gaaattaccg ggaaagcctg ttttgtggaa
      421 gcctttagat gaatatgcgg ttgtagataa tacggagcaa ataaacgaag cctttcattc
      481 tgtagttggt catgacggaa gtctgagaga ggtcattcaa aaatgtatag ccgctgtcaa
      541 atatccgcct aacggactca gtgtattaat aaatggagcc acgggtgttg gaaaaagttt
      601 tttagccaca aaaatttttg aatacgctat ccatgagcaa attattgaaa aagatgcacc
      661 atttgctatc ttaaactgtg ccgattatgc ggacaatccc gaactgcttt cggcaacact
      721 gtttggatac aagaaggggg cctttacggg ggctgaaaag gataccgatg gtttattggc
      781 aacggccaac aacggctatc tttttctcga tgaggtccat cgcctatcga aagaaaatca
      841 agaaaagctc tttctattta ttgatactgg aaactatcgt ccagtcggtg aaaatgtaaa
      901 ttggcattct gctaaggttc gttttatttt cgccacgacg gaaaaaggtg aaaattattt
      961 attagacacc tttgatcggc gtattcaaat tagtgttatg ttgccaactt ttgaagatcg
     1021 ccccattcga gaacgattag agctgattca attatttttt caaaatgaag cggatgtttt
     1081 acaaaaagat attattgttt caagagaagc cttgtcttta atgttatctc atccctttag
     1141 tgggaatatt ggtaagctga agaatatcat taaaatcagt tgtgcggatg tgtatagtcg
     1201 aacacaagaa gaaccattaa cgattgggaa aaatgagatc ttgattcagt taaatatgct
     1261 ggaatccgaa gtagaaagct tgccgattgc tagtttattg ataaagaaaa atcaaaagat
     1321 aaaaacggat cgaatcaaac cgtttgatta tgattcgatt ttggaaaaaa ttactcagct
     1381 gataaatgaa ggtaatttca atgaaaattt ttcttcgata aagaatgaag tacagcattt
     1441 ggtgcatcac attgataagg attctttttt atcaatgagt agaaaattga tgattcagat
     1501 gtttagaaag gtctgggtat ccgttttagg aaaaacgtac ggattgacga cgagtggacc
     1561 tgttgcagaa attattagca gatcctatgt catttcccca agctataaga cagacttaac
     1621 cgggataaat cgtacattag ctgcaaatct acctcggacg acttacttat gtaatcagtt
     1681 cttggagcat ttaccaccgc tctcagaaaa ggacgagaac tttctaagac tgcttattgt
     1741 cgtgacttta tctgattatg tagatgaatc catcgagctg aagggcctgt tgcttgctca
     1801 cggagatagt acggctagca gcattcaagc agtagtgaat caattatgcg gcaattatgt
     1861 ttttgaagcg ttggatatgc cagtagagag taatgtgtca gagattgtcg aatcgacaag
     1921 gaatttcgtg aaacggcaag tgaatacaga tagtctagtg ctgatcgtag atatgggctc
     1981 tttaaatcaa atttacacac agatcaagaa cgatttaaaa ggggagttac ttcttctgaa
     2041 taatttgacg acttccattg cacttgatat tggcttgaag atgacgatga atgcgccatt
     2101 taaacagatt gcggaggatg cagcagacaa gtattcaatc aatgttcaat actttgaagg
     2161 cttttctcaa agtagcaaca ttatcatttc ctgtatgtcg ggtttgggga tttcggacaa
     2221 attgaaagat gtttttcgtc atcatttatc ggatgaatcc atcgaagttt tcacgaagga
     2281 ctacagagaa ctacgtgaat taatcgacca aaatgatgaa acttattttg acaagacaaa
     2341 attggtcatc acgacttcgg atcttccacg ttccttttca attcctaata ttaatgttta
     2401 tgacatctta gacggtgatg gagcagccaa tttggagcat attttgagtc ctgaaatgaa
     2461 gccggcagaa tttaaccgct tgattcagga gctagtaaaa ttcttttcgt tggaaggaat
     2521 tgcggaccgt ttgaactttc tgaacccaac gatcgtgatg agtgaagtgg aaacagtcat
     2581 ttctaaatat gaaaattact atcatttcac tttaaatggc cgtgtaaaac taaatctcta
     2641 tatgcatacg gcattgatga tggaacgttt attcttggct cggacagaaa aatctgaaga
     2701 accgaattat gatttgaaac cagatgaaga tgaattttat tatgtttcta gaagtatttt
     2761 tcaatcaatg gagatgaaat ataattttaa agtcaatgct tatgagcttt cgctgctcta
     2821 tgaattatta ggaccgtata ttcaaaaata aagtgtctaa aaaagtgtct aatctgtttt
     2881 ttttaggtta gacacttttt ttaatgtgaa ttaacgtgat taaaccaatg tttttaaaag
     2941 ttggcacagg aattgcttat ataaaagtga acagaaagaa aggaattaac catatgaaga
     3001 aaattctaat agcgactcac ggtcacttag cttcaggctt tcttagcagt attcaattac
     3061 tgacggggaa ggttcaagag attacggtga ttaatgctta tgtggatgag tgtgattttg
     3121 aacaggagtt gaatgtattt gtaaaccagg tgaacgagca ggatcagatt tttgttttta
     3181 ccgatttatt tggcggaagt gtcaatcaga agattaccaa aacctttttg gagaaagaga
     3241 ttgctgtaac tattttgtct ggctttaatc tgccaattct gctcgaaata gtattggcaa
     3301 ccaaggaatt aacaaaggag gatattcagg aactggtaaa taaatgccaa caggaattaa
     3361 agatgaccat tcttgatgag gtacatgtag taaatgaaga ggaagatttt ttttaggagg
     3421 aagaaaaatg attaaagcag tgcgaattga tgaacgatta attcatggac aagtagcgat
     3481 gacttggaca aagagtctaa atttaaccgg gttagtagta gcaagcgatg aggcggctac
     3541 caacgatatt caaaagatga cattgaagat ggctgcacca tcgaatgtca aagtcattat
     3601 taaaacagtt gatgacgcga ttcatttgtt aaaagatccg cgggcgaagg acatgcgctt
     3661 gatggtttta gtccctaatg tcaaagatgc ggtaaaagtt gcgaaagctt tcccaacgga
     3721 aatcgaatta atgaacgtgg ggaatgctgg gaaaatgaca ccgccatcgg aagataagaa
     3781 aaccttatca aaggaagtga tgttaacgac gtcagaaatt caagacttaa aagaattagt
     3841 tgaactttat ccagataccg tttttcagcc tacaccggcg atggaaaaac gttcagctat
     3901 ctctattttg agtgattttt agaaaaagga aggagaaaaa ttatgtatga tgcatttgta
     3961 gtcgctttgg ccgtttttat tggagttgct ggacatgaaa tgttcgggat gtccatgctg
     4021 agtcgtccga ttgttgttgc tccgttagca ggagcgttga tgggggattt gcagacgggg
     4081 ttgattgtcg gggcgtcact agaagccatc tttatgggtg tcgtcaatat cggaatctct
     4141 agtacggcgg agccttcttt ggctgctggg ctggctacag cgttcgcaat caaaatggga
     4201 ggaaacgtgg atgcgattat tcccattgtc tttccactag cagtattagg attacagtta
     4261 atgaatatga ttttttcttt tgtatgtgga ccgatggctg ctaaattttt caaatttgca
     4321 aaatctggtg atcaaaaagg aatcatcaat ctgcacttct ttatgtggtt tgtccactat
     4381 gcgctttatg ctcttattcc attctttgcc gttttatttg gagcagatgt tgtcaaaagc
     4441 gtcttggata atattccaga ggtcatcatg aacggattaa cagtcgctgg aaatctgctg
     4501 ccggcagttg ggatggcgat gctgttacgg atgctttgga gtaaaaatat tgctgtgtgg
     4561 tttttctttg gggccgttgt tatggcgtat ctcgagttac cattgattgc aattgccgca
     4621 gttggaacga ttattgcaat tgttatagcc caaagagatc tacaactaaa aaatatatct
     4681 gttcatgcag ttcaaagcga tgctgctgta aatgcaacgc ttgaccaaga agaggaggat
     4741 ttctttcaat gaaattagct gatggaattt ctaaagatga gaaaaaattg gttcggaaga
     4801 tgttttggcg ctcgatgact atgtatgctt cggtgaatcc ggtgacgatg ggcggcggtg
     4861 gtttcgccta ttctatgatg cccttcatta atcatttcta caaaagtgaa gaagatcgag
     4921 ctgcggcgct ggaacgtcat acacagtatt tttcgacgac cattccttgc gcgtcctttg
     4981 tcatggggat tgcggcatcg atggagaagg aaaattctga aaaagctggt tttgacgtgg
     5041 aaagtatcaa cgcgatcaaa acgagtttga tgggaccatt ggctggaatt ggcgattcat
     5101 tgttctgggg agtttggcgt gtcgttaccg cgggactagc gatcaactta gccaatgccg
     5161 gaaatatttt ggcaccaatt atctttttag ttatgttcaa tatacctaat tacttgatgc
     5221 gatactatgg cggcgtttta ggctattcat taggctctaa atatattgaa aagctgtatg
     5281 cgaacgggtt aattgaaatt ctgacaaaag ctgctagtat cttgggtctg atcatggtag
     5341 gtgcaatgac gatcagtatg gtcaaattca ataccgtttt gagcttttca atgaagggcg
     5401 aagaactaat gaatgtccaa gaaatcctcg atagtatttt taaaggaatt gtaccgttat
     5461 tggtaacatt gagctgtttc aaattactag ataaaaaagt cggcgtcatc aaaattattt
     5521 ttggaatggt cgtcttggga attttattgt cattacttgg aatcgcatag taggaagctg
     5581 cagctactag gctgcagttt ttttagcaag gagggaaaag atgactatta atgaagtaat
     5641 ttattcaccc aaacacccat tttttaatgc tgagttaaac gacggaaatg tgactacttc
     5701 aaacgaatac agctactatc ggaatattcc tgatgtaaag ggattgacct tcgatgtaag
     5761 taatcaaaag acattagcaa accttgatgt ttgcgggacg attcaaacgt tgacctttta
     5821 tcgagaaaat ttactaactg aggaaaaacc aggtgtttgg gtcaataaac agctctctca
     5881 aaccaattcg ttatccttaa aggttgaagt aaacgggatt gtctaccatt tgtcagaatc
     5941 ggatcatcgt attgaagtcg atgtgattca agactgtttg cctagatata ttcatcatta
     6001 ttcgacgttt aaggtcattg tagtatcctt tgctcccata ctggctaata agcgtttaag
     6061 tatgcttgtt caacaggttt acattgtgaa tgaaacacac gaaagccttc aagtttcagt
     6121 gaaagatgtg ccttcgtatc aagagaagta ttcggatcag caaaatgtgt tgatttctca
     6181 aaaaggaaat aaggacacga tcgcacaaca ggaagtggca agcttcgcca ttgcttttgt
     6241 ggacccaaat gcttttgaag aagtaatgac ttttcaggaa tctgatatcg aaaaatggct
     6301 gaaggaaaca ttgaactatt ttgctcagct atttgggcag ctgtcaatgc caaacgatag
     6361 agttgtccat ctctatcgtc gtgcacttta tcaaagcttt tctagtttcg gtatgaatcg
     6421 acagggacag gtcgtcggca gcaattgggg cagctatcct gctactaatc gaatttggaa
     6481 taaggatatg tattacgcat cattgccatt caccatgttt gaaccggagt tatgtcaaaa
     6541 aacggttcta tggtttgatc aatatggcgt taagtttcct ggttcaaaat ttacaggagg
     6601 tatcaatcat tctctgagta attcgttatc cagtgcgtta ctggctagtc tttattttga
     6661 acatactggg gatacaaaat tctttgagcg gtgtccgcag gttctactga atgcttcaag
     6721 aattattgag gacatattag ctcaacgtcg gtcaggggaa ccaatgcttt ttccatctgt
     6781 ttggttatca gacgcctttg cacttggaaa gtatcatacc ggcagcaatc tttgtctttg
     6841 gaaggcctgt attggacttg cagaaatttt tgaagcccta gaacaactga gtcttagcaa
     6901 gcgatacaaa aacattgcct gtaaactaaa ggaatcaatt tgtaaacaaa tgactatcga
     6961 tggtcctttt ggtgagcaat ttttggaggg aatcggtgat gaggagaaaa caacctattc
     7021 agtgaagcac tatcaaaaac cgattcttga gcaagggtta atctttttaa gtgatgtgat
     7081 tgtggatgga aaaattaatt tattgatgca tgatggagaa gaatcagaca ctacgttgat
     7141 gcctttttat caatttttag ataataaaga tcatttatat cagaacacca tggccttttc
     7201 tgctagttct ttcaatccaa cctatagcga ggaaatcaaa ggcatcactt ggggattaga
     7261 atccggcgcc acttttcctg gctttatcac tgttttaatg agcgagctgc acaaccctca
     7321 agctttcgta acccgattag aagaactaat ttgtttggca gatttagacg gttcgtggtg
     7381 gtggtggccg tatcgcttag aggctcaaca aggagaagtc gtgcgagact ttggctgcgg
     7441 caaatgcggc tgggcatcgg gtctttttgt cagcctaatg atcagtcaat atttcggatt
     7501 gaaattcaaa aaaggagtat tgcaaattga tcctgttgat aatttaacgt tttcttggaa
     7561 gaatttgaag ttcggtcaag ccttcatttc gattgaatgt acgcaatccg agttgagtat
     7621 ttcaaatttg tcagaaaagc cattgaaaat aagtgatacg aaaaaaattc ttcacgtaga
     7681 aaagggaaaa acgattcaca ttcaaaggag aaaaagatga gtatactaaa aaatattacc
     7741 caatttaaaa aaatccagag tcgagcaatc actcccgaaa atccaacagg cgagaaaggt
     7801 aaagctgcga tgttgccaag cgagctagga ccaagccgca aaggtcgtgg cgcaattccg
     7861 ctgacttctg gtgaagaaac tgtgattgcg gatattactg gaacgggaat aattcgtcat
     7921 atgtggatga cgattcgtga tcgaacagaa tttggcagtt ttgttttacg tgatttaatc
     7981 ttaagaattt attgggatgg gtcggatagc cctgctgttg aaagtccttt aggggatttc
     8041 ttttgtaatg ggtttggaga gcgttgcgag gtgaactcgt tgccgatagt ggttaatcct
     8101 acagggggct ttaattccta tttcgaaatg cctttccaaa catccgctaa aattacgatc
     8161 gagaatttac atccgaagga tatgtcctca tttttttata cgataaatta cgctttggtg
     8221 gacgaattgg aagaaaatag cctatatttt cacgcaaatt ggcatcggga acgcattacc
     8281 aaattacagg aggattataa gttaattgat cgattaaagg gtcaaggcta ttacgtgggt
     8341 acttaccttg cgcttacctg cctagaacgt tattggtggg gcgaaggcga gtttaaattc
     8401 tacattgatg gggatagtga gtatccgacc gtaacctcaa caggttcaga agattatttt
     8461 ggcggggcgt gggcctttca tgagtacgat gagttcggac gtccacatgc gaaaaactat
     8521 tcaacagcct ttttagggta tccttaccaa tcaaatagag atgccacaag agatcgtttt
     8581 gaaacgggaa aattgaatgc cgttcatgcc tttggtgacg acgcaatgcc acgacatggt
     8641 ctttatcgtt ggcatcttca agaccccatc tgttttaccg aagatttatc ggtcacttta
     8701 caacaaatcg gcaacgatga tgttcgctta tttgagcggc aagacgatgt tgctagtgtc
     8761 gcttactggt attcaacaga agcttcagga aatactaaac gtcatttcac tgatcgagaa
     8821 cgcttgccgc gttaatgaag tgtttttcac atattcgaag ggaggaacct atgatgatat
     8881 atggggcaat tgaagctgga gggactaaat ttgtctgtgc agtcggtact gaggaattaa
     8941 cgattttgga aagagtcagt tttcccacaa caactccagt tgaaacaatg aagcaggtca
     9001 ttgatttttt taaacctttt aaggaacaat tggcaggcat cggggtcggc tgctttggtc
     9061 caattgatat tcaatgcaaa tcaacgagtt tcggtcatat cacatcaaca ccaaaacttg
     9121 cttggcaaaa tttcaatttt gtaggaacgc taaagcaata tttcaatatt ccgattgaat
     9181 ggacaacaga tgtcaatgcg gcttgctatg gtgaatatgt tgctggttct ggcaaagggt
     9241 tagcaggtgt tgcatacttc actgtgggaa caggtatcgg aggtggtgcc ttgttcaatg
     9301 ggactttcgt ggaagggttc agtcatccgg aaatggggca tatgttgatc aagcgacatc
     9361 caaaggacaa tttttcagga aactgtccct tccaccaaga ttgtttagag gggatggcag
     9421 caggaccagc gattgaaaaa cggttgggtg taaaaggcca gaatttattg gctgacgatt
     9481 cgttttggca gattgaagcc ttttaccttg cccaatgtgc atacaataca acactgatgt
     9541 tttcgccaga tagaattatt tttggcggtg gtgtaatgaa acaagaacac atgaagaaaa
     9601 aagttcagga taaatttgtt gagcttataa atggatatgt tgaaatacca ccaattgata
     9661 gctacattat tacgcccgaa ttaggtgata atgccggaat aatcggcgga ttagcattgg
     9721 ccagaaaagc ggtaagaaat aagcaaccat aattaatgaa tgtttcattg atactcatgt
     9781 caaaagtatt taaccgagta atcaaatgat caaatgaagt tggataaagc gaaaatgaat
     9841 tcaaataaaa aggctctaag tcaaatcgtg ttgttttcat taaatcactg aaaggaaatg
     9901 cttctgcgtt tccttttttc tttttgttgt gcgctgtata ccttgaggat attcactggt
     9961 ggcgtacact taaatagtaa tatattgtgt tactttatgt ctgtggtaat agttatagtt
    10021 gcttaccaat gtatgttcat ttttcgaaat gttgacttca taatccttgg cttaatgttt
    10081 gaattttttt ggtgatagat tttttcagcc gtataaaata aatcgtaatt ctattataga
    10141 gagttgtgat tataaaaaga tagcaagaaa aaaaaagaaa gtttctatac attttaaata
    10201 ttttattcct atttctattg ctaaaaaagt agaaattcta tatttacctt taactctcgc
    10261 ttctcttgca aatgtagtta gcattttaat cgataataaa ctcaggtagt tatattacct
    10321 tgaaatgatt gataattttg tcacttctat tttttttgca ataaattttt tattttagtg
    10381 aaaaataaga aactatttct ttgaaggcga taagttagaa aaatattatt gtagtctttt
    10441 ctttattagg aatcatcact gttgtatctt attacctttt ctgtttttct tattagagaa
    10501 aaataatttg agaggtagat agtt
//
LOCUS       DACEZN010000092        10478 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEZN010000092 DACEZN010000000
VERSION     DACEZN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.092"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            35..658
                     /locus_tag="IXH60_001871"
     CDS             35..658
                     /locus_tag="IXH60_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990781.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1108..2157
                     /locus_tag="IXH60_001872"
     CDS             1108..2157
                     /locus_tag="IXH60_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1990782.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2154..3035
                     /locus_tag="IXH60_001873"
     CDS             2154..3035
                     /locus_tag="IXH60_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990783.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3038..3808
                     /locus_tag="IXH60_001874"
     CDS             3038..3808
                     /locus_tag="IXH60_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1990784.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4087..4941
                     /gene="aroE"
                     /locus_tag="IXH60_001875"
     CDS             4087..4941
                     /gene="aroE"
                     /locus_tag="IXH60_001875"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1990785.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5119..6282
                     /locus_tag="IXH60_001876"
     CDS             5119..6282
                     /locus_tag="IXH60_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1990786.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6399..6809
                     /locus_tag="IXH60_001877"
     CDS             6399..6809
                     /locus_tag="IXH60_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1990787.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6983..9211
                     /gene="topB"
                     /locus_tag="IXH60_001878"
     CDS             6983..9211
                     /gene="topB"
                     /locus_tag="IXH60_001878"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1990788.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9349..9666
                     /locus_tag="IXH60_001879"
     CDS             9349..9666
                     /locus_tag="IXH60_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990789.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9826..10062
                     /locus_tag="IXH60_001880"
     CDS             9826..10062
                     /locus_tag="IXH60_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1990790.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10059..10457
                     /locus_tag="IXH60_001881"
     CDS             10059..10457
                     /locus_tag="IXH60_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1990791.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3655 a         1761 c         2222 g         2840 t
ORIGIN      
        1 ataataaaaa ataggagaaa aagtagaaaa gaaggtgttt gttatttggg aagtagttaa
       61 atcgagagta caagaaatga atgaacattt ctcagtgtca ttcgcagaaa atccaagtaa
      121 gtattttcga cgtcttgttt ttctgctcat cttatcattg ctgttaacag ttgcaatgtt
      181 tttattgccg gaaaaaatcc gagaagagca agtaaatagt ctcgtttatt cgtccatacc
      241 aaccagcaaa ttgaaacctt ttgattacag caaagctgat cagaaaatca aacaaagccg
      301 ggcgatatcg gtgatgttca gcaaaccgaa cgggaaaacc tatcaggaca tgcttgatgt
      361 ttttaatgat cctgagctta tggaagaatt gaatcgaccg atcttttatt atccgatcgt
      421 ttatgatgta catgaactgg aacaaaaata taacatacga acggatgaag tgacatttat
      481 tttctttgat ggaggaaaag aagcaaatcg gataactgcg ggtaagggag gaataactga
      541 ttttgataag gagttgatcc ctgaactgaa tcgacttccg ttagccaata tcaaacaatt
      601 agaagaagaa ttggatagaa cgggagtaac ttcccaaaat tccgagattg ctaattgaga
      661 cattggcgcg atgaaacggt gggtctatat agcgctattt ttaattatca gaacaagtga
      721 tgtttgatct tcaaaataac aaataaaaaa ttaaaaaata tagttgacga aattcaaaaa
      781 ttcgattaga cttaaatcaa ttcttaagga aagggtgacc tgtaatgaac ttatcaaaac
      841 gtatttcagc agaaaacaaa aaaaattggc gcagctggcg tagataagtt gtattaatgg
      901 tgagactttc ccatagatac aactttttag agtgcatttc taaagcttgt atctatgcta
      961 gacatgaaca ttgactgccg catagattag aatctatgcg gttttttgtg taaagacaat
     1021 gaccgctttg gccattgtct ttttttgtag actggatact tgcctgatta taataaataa
     1081 aaagattgac tttgcaggag gaaaaaaatg aaaaacaaac gattaataac agttgtagta
     1141 attattttag tttatttagc aggtgcattt atttatggaa aagcctcagg cgaaaaagaa
     1201 agtacttcga cgaaccagac aaaaaaaaca acagttgttg gggtattgca atacgtgagc
     1261 catccggcat tagatgagat ttatcgagga atcaaagatg gtctgaaaga agaaggatta
     1321 aaagaaggag aaaatcttga gatcaaattt caaaatggac aagccgatca gagcaagcta
     1381 gccacaatga gtcaacagct agtacaatct gatccagatg ccttgattgg aatcgcaaca
     1441 cctgccgctc aatcattagc aaacacgact tcatcgatcc cagttgtgtt aggtgctgta
     1501 acggatcctg tgggggctgg attagtcgct tctttagaga aacctggtgg taatgtaaca
     1561 ggtgtttctg atcagccgcc tgttacttca caaatcaagc ttgcttctga tttgttgcca
     1621 aatgcaaaaa aagtcggaat cttgtatgct tcttcagagg ataattcaaa ataccaagta
     1681 gcacaagcag aagaagctgc aaaaaaagaa gggttagaat cagtaaaata tgcggtgcct
     1741 tccactaatg agattgcaca aacggttcaa gtgatgagcc gacaagtcga ttttatctat
     1801 gttccattag ataataccat tgccaatgcg atgcaaacag tggtaaaaga agcaaacaaa
     1861 gcaaatatcc ccgtcattcc atctgtggat acaatggtgg aacaaggtgg actagcaaca
     1921 atcgggatca atcaatatca attagggctt caaagcggta agatggcggc agaacttgcg
     1981 agtggaaaag aaaaaccgga aacgacgcct gtttatacat ttgatcaagg ggatacagtg
     2041 atcaatcaat ctcaagctga tcgcttagga atcattgttc cccaatcgat gaaggaaaaa
     2101 gccaaaatca ttacagatga ctcgcagcaa gaaacatcta aggaggatga caaatgatcg
     2161 tatcagctat ttcacaaggc atgctttggg caatattagg attagggatt ttcatgactt
     2221 atcggatctt agactttcct gatatgacga cagaagggtc cttcccatta ggtgctgctg
     2281 tctgtgtgac agctattact catggggtag cgccgattgt agctacatta ttaggcgtag
     2341 gtgctggcat gatagcagga ctcgttacgg ggttacttta tacaaaagga aagataccgg
     2401 ttattttagc agggatttta gtgatgtcag gattgaactc agttattttg tttgtgatgc
     2461 gaacaccaaa tctttcttta ttgaatcatc cgattctaca agacagcttc catcgctttg
     2521 ggttacctga ttactttgac acagtccttc ttggcttcat ctctttgggc atcttgattg
     2581 ctgtactgct attcttcttc aatacagatt tgggtcaagg ctatattgcg acaggagaca
     2641 acgaaacgat ggctcggtcg ttagggataa aaaccgatcg tatgaaaatt ttaggtttaa
     2701 ctttgtcgaa cggtgtcatt gcattatccg gtgcgctgat tgcccagaac gacggatatg
     2761 cagatgtaaa taaagggatt ggcgtaatcg tcatcggtct tgcttccatc atcatcggtg
     2821 aagtgatctt ccaagaattg acgctagcag aaagattgat tgctattgtt gtcggcagta
     2881 tcatttatca gctattgatt ttagtagtaa tcaaactagg gtttgacacg acttatctga
     2941 agttgttctc cgctatcatc ttagctattt gtctgatgat cccgcaattg aagaaagcac
     3001 tgaacttaaa aacgggtttt gagaaggagg catagtaatg actgatatat tgacaatcaa
     3061 aaatggcaga aaagtcgtaa acaatggaat gaatgaagag aaagtactat tggatgaaat
     3121 taaccttaca atcagagaag gggacttcat cactgtcctt ggcgggaatg gtgctggaaa
     3181 aagcacactg ttcaatacga tcgctggtac gcttcaactc agtcaaggat cgatctcttt
     3241 taaagatcag ctgatcacaa aagattcaga agaaaaaaga gcacgattcc tttctcgagt
     3301 tttccaagac cccaagatgg gaacggctcc tcggatgaca gtagcagaaa atttgctact
     3361 tgcacaaaaa agaggtcaaa aacggaaatt ccgtttacgt aacttgaaaa atcaaaaaga
     3421 agattttttc agactatgtc aagaagtcgg taatggccta gagcaacact tggatactcc
     3481 agcgggtgaa ttatctggtg gtcaacgcca agcattaagc ttgctgatgg cgacgatcac
     3541 agctcctgag ttactattat tagatgaaca tactgcagct ttagatccta aaacagctaa
     3601 agcattaatg cgattaacaa atcagcggat caatgaacaa aaattgactt gtctcatgat
     3661 cacgcatcga atggaagacg ccttgaaata cggaaatcgt ctgatcgtac tccaaaaagg
     3721 gaaaatcgtt caagatattt ctgaaaaaga aaagcaacat ttgacaatgg gcgacctttt
     3781 gcagttcttt gaagaagtag tggattgatt tcgacagaaa aattcctttc agtagaagga
     3841 cttgaaaact cattgacatc ttcctttgat tcatcctata cttaaaacaa taaacaatta
     3901 aacatatttg ggtgttttgt aggatcacgt agtcaatgat tttaacaatt aaatagaata
     3961 ttctttgctc tgacaagtaa acgatgagaa aagaatttgc tactttgcca cctagacgtt
     4021 tcgtactttt tcgtatcaaa cgctaagtgg ctttttttat tgaagtaaga acaaaggagg
     4081 aataagatga tttctggaca aaccaaatta gctgggctct ttgctgatcc ggcttctcac
     4141 agtttatccc cattgatgca caatactgct tttgaagcaa acaacatcga tgcggtgtat
     4201 ttagctttcc aagtaaatcc aatgaatttg aagcaagcag tcgaaagtat tcgaactttt
     4261 gatatgttag gtgtaaatct ttcgatgccg aataaaacag cggttattcc ttatctggat
     4321 gaggtaagcc aggaagcgca attgatcgga gcagtgaata cgatcgtgca cgaaaagggt
     4381 cgtttgattg gctataatac tgatggtgcg ggcttcatgc aatccgtgca agaagctggg
     4441 atttcgatca aaaagaaaaa aatgactcta ctgggagctg gaggtgcagc aaaggcagtc
     4501 actgttcaag cagcgttaga cggcgctgag gaaatcgtag tttataaacg aaacaaccaa
     4561 acattcgctc aagtaaaaga aacattcgaa caactcagct atcgaacttc ttgtaagata
     4621 aaagttcgtg actttgccga tacgcatcaa ttaagaaaag acttgaaaga aagctgtctt
     4681 ctagtcaatg caacagatat cgggatggga aagaaaatca atcagtcacc gctcttagat
     4741 ataagcatga tgcatgagaa tctagcggtt tttgatttga tttatgcacc tagagaaaca
     4801 cgtttattaa aagaagcaaa aaaagtagga gcaaaaggat acaatggact tggcatgttg
     4861 atctaccaag gagcaatcgc gtttgaatta tggacaaagg aaaagatgcc gatccatgtg
     4921 attgaaaagc tgtttgatta aaaaaggata atagataaag atcttttatt ctcgcataaa
     4981 cggtctcaag aaaagtaatc ctttatgtat cgaagcattt atttcgttat tttttattaa
     5041 attatcaaaa aatgataggc ttttgtcgat tggttatcgt atcctttatt tatcagtaag
     5101 aaacgaggag gaaccacaat gagtcggatg gatcgctata aggacatcca taagaaggcg
     5161 aaacctctga agcgggatac ggggagtatt tttgctagaa gagaacgaaa acgaacgcgt
     5221 atggaaaatg aggaagatgc tacccgtatt tatgaaagag agcagcattc atttgaacat
     5281 ttgaaccaat acgagcaaca gatatacaga gatgcaccaa gagcagaaga acaacaaaaa
     5341 aataaaaaat ttttcaatcg gcaaaagaag aacaaaccgt taaaacctcg aagaaagcga
     5401 tcgtggaaaa aaatcattct atcgattttg cttgttttga tcctttattc aggtatctca
     5461 ttttttattg gtaaatatgt agctgaacat gacacaagct tagaggcagc gacttcagaa
     5521 acctttcatg ggcaaaaagc gacgaatggt gcgcataata ttttgatcct tggaagtgat
     5581 acacgcggag aagatgccgg gcgagccgat acgatcatgg tcttacagtt ggatggtcct
     5641 tcccataagc cgaaactgat ttcttttatg cgtgacagct tcgttacgat cccaggggta
     5701 ggacaaaata agatcaattc agcttatgca tacggaggag ccgatctagt gcgtcaaact
     5761 ttagtagaaa attttggaat cgattgtcaa tactatgcga aagtcgattt caaatcgttt
     5821 gaaaaagtga tcgatgcctt attcatgaac ggagtgaaga tcgatgcaga aaaagatctg
     5881 aatctggatg gtgtagatat taaaaaaggg ggccagaaga tggatggaca tgttcttttg
     5941 cagtacgcac gtttccgaat ggatgaacaa ggagactttg gccgtgtcag acgccaacag
     6001 caagtaatga acgctatttt cagccaattg aagaatccat tgaatctgat ccgtgcacct
     6061 tatgcagctg gtaaagcttt aggatataca tccacagatg tcactgcttg gttcttgacg
     6121 aaaaacctct tttcgattgc acgaggagct ggtggcattg atcgaataag tgtgccagta
     6181 gacggttcgt ggaattatgg gcaaagtgat tatgctggca gtattcttga aattgataat
     6241 caaatgaatc aatctgctat tacagatttt ttgaataaat gatcataaaa aagatcaaga
     6301 agaagctgcg caaggctttt gtcatgaatc aatcaaacga ttcactcgta aaaaacgctt
     6361 tcttatggta gatttaacca agagaggagc tgtttacatt ggatatctat ctgaacttca
     6421 aaaatgagtc gcgtgaagcc atcgcttttt atgaaaaagt ttttcagact gaagcatcag
     6481 gtatcaccac atacggtgaa atgcctggaa tagaacaaat gaacttgcca gaagaagcaa
     6541 aaaatctaat cacgaatgca cggctgaaca tttatgatac attagttatg tttgcagata
     6601 cgccagaagg aattggtcct gaattagttg tgggaaataa tgtttcttta gtcatccaga
     6661 caagaaatga agaagaaacg aagcgcctat ttgaaagctt agctgagggt ggtaagattg
     6721 agatgccact ggatgctgtt ccgtgggctc agttgtatgg gatggtaact gatcgatttg
     6781 gcatcaactg gcagattaac caagagtgac agctaaagat gaatattttc tggaaacatc
     6841 aagcagcaaa cggatcatcg tttgtttgct tgatgttttt gtgtctattt cgactatacg
     6901 gtatcttttt tttattgcca gatgcgaaaa tatgttccat aatagagaag tccgcaaaaa
     6961 attaagtaac ggaggtcaga agatgaagac ggtgatacta gctgaaaaac cttcacaagc
     7021 aaaagcctat gccgaaacct tccaacagca aaagcggcat gaagggtatt atgaaatcag
     7081 cgatccattg tttgctggtg aagtaaccat cacttatggt tttggacatt tggtagatat
     7141 ggttgctcca ggagcttatg aagagcgttg ggcaaaatgg tctttagaaa atttgcctat
     7201 tttcccagaa accttccgtt acgaagttcc aatggacaaa cgtgcacaat tttcgatcgt
     7261 aaaacgagaa ttgcagtcag ccgataccat cattattgca acagatggtg atcgagaagg
     7321 tgaagccatc gcgtggtcga ttattcgaca agcaaacgca ttttctaagg aaaagaacta
     7381 tttacgttta tggatcaatt ctcttgaaaa agaagcaatc tatgaaggtt ttaagaatct
     7441 ccaacctgga aagaactatt ttccaaaata taaagaagcg caagcaaggc agaacgctga
     7501 ttggctgatt ggaatgaatg gaagtccatt atatagcttg ctacttcaac gaaaagggat
     7561 tgatggcagt ttttctcttg gtcgtgtaca aacaccgaca ctctatatga tttatcagct
     7621 gcaagaagaa atcaaacact tcaaaaaaga aacgtacttt gaaggagaag gtttaattac
     7681 gacaaacaaa gggcagtttt ctggaaaaat cgtaccaaaa aaagcattta aaacccaaga
     7741 agagttaacg aaggaaatag aaaaacttgg agcgcattta ggtaagcaaa acggacggat
     7801 tcttgaagtt acgaaaaagg aaaagaggtt gcatagtcca cgtttatttt cgctatctag
     7861 tttgcagaca aagatgaatc agctaatgaa agcaagtgcc aaagctacac ttgatgctgc
     7921 tcaaggattg tacgaaagca agtttctaag ctatccaaga acggattcct tttacattac
     7981 agaaaatgag catcagtatc tggttaaaca tctgcaaaat tataaaaaat ttttaggaat
     8041 agaagaagta cagacaactg agatcaaagc aaagaaacga tatgtcgatg cgaaaaaagt
     8101 tcaagaacat catgcaatta ttccgacaaa gacgattccg actcctgcac gctttgcgaa
     8161 gctttccaaa ttacagcaag caatctattt gcaagttttg cgcactactg tggctatgtt
     8221 tgctgcagat tatatctacg aagagacgac cgtaatgacg ggcgtacagc aattaaagct
     8281 aaaaagcatc ggaaagatcc cattacagca aggttggcag gaaatcttac gcatcaagtc
     8341 aaaaaagaaa gaagctcaga ctcttcctcc tatcgtagaa aatgaagaag cagaagtaga
     8401 actgaaaagt atggagaaag aaacacaacc tccaaaaccc tataccgaag gaacgttgat
     8461 cacagcaatg aaaactgcgg gaaaaacgct ggataatgat gaagcgcaag aaattctcaa
     8521 agaagtagaa ggaataggga cagaagctac acgagccaat atcattgaaa atcttaagca
     8581 gaagcattac atcgaagtca ataaaaacga aataactgtt acgccaaaag gaatcactct
     8641 ttgtaaggca gtagccaatg aaccattact gacgagcgca gaaatgactg cgcgctggga
     8701 agggtacttg agaaaaattg gtaaacaaga ggggacacca tctgtttttt tagagaatat
     8761 caaaaaattc atcctacatt tattggcaga agttcctaat caaatcgaca aggtagactt
     8821 ttcagatgag attagcggga cacgtcagat aaaagcgatg gagaagaaaa acgatcaatt
     8881 aggaagatgt ccaaaatgta aaaagggaat agtcatgctt tatcctaaaa tcgctacatg
     8941 cttgaatccg gaatgcgact tcaaattgtg gccaacagta gctaaaaaga agctgactaa
     9001 aacgaatctc agagatttgc tttcaaaagg aaagacaagt aaagttgtca aaggttttac
     9061 tggtaaaaaa gggaaattcg atgcagtttt agtattaaaa gaagatatga caatcggttt
     9121 ttcttttcca tggatcgaaa acagtggaac aaaagcagtt gatggaaaca aagaaacatc
     9181 aagaaaaaat aatgaaaacg aagtagagta aatgaaaaca atgaaatagc ggaatattta
     9241 ttccgaaaag tgagaatata aataccaata aaactaaaaa atatcagtga atcaagcaaa
     9301 aacgttgggg taggctgatt ttatatagat agcaaggagt ttttttattt gaaaaaagtg
     9361 cattcatttt taaattatct tatgggagca atcacgattg tggctgtgta ccaagcattt
     9421 ctaaagtatc ctgattacgg gacgaagttg ttgtcattga cgcacaatca aacagcatat
     9481 tcaccaacgt tgattttatc gactttgact gcaggagcta aaagcgctac ttattttttt
     9541 cataaaacga caagcatgct aaagcgaaag aaacagcgaa aacaattgtt ggtggtggta
     9601 gcggctatga tcattattta ttttctacca gttattacac aagtatttca tcaatatttt
     9661 ggttaagaat acgagagttt gtgacaaaag tcatgtcaca aacttttttc tttgtcattt
     9721 tttcgtaaga aaaagcaaaa taaaagtata tacattgatt aaaagtatat acttggatat
     9781 aatgtactta ctatagaatg agaatggtat agagggtggc gaaatatgaa aaaaatgaaa
     9841 aagtataaaa ttagaaaatc tggcaatagc gatgtaacaa ctatcccacc agaagtaaaa
     9901 gaattcatgg gtgtgcagac aggtgacgca atcagttatg tttttcaatc tgatggttct
     9961 gtacgaatga tcaaagcaca agaagaacct gatattgatt cattagttga ttcgatcatg
    10021 aatcaatatg aagacgcgtt aaaagatttg gttgatttat gaggtatctt tcgactaaag
    10081 aaattatcaa aatcaacgcg aaagttattt tacgtcactc gccaggcgaa atgatcggtg
    10141 taaaagatgc aaatgcatta gatatggccg tcaaacagcc ttcacaagca gtattcaatc
    10201 aagaactcta tcctgaaatc tatgaaaaag cggcgatttt agcaattaat ttagctaaaa
    10261 aacatccttt tcataatggc aataaacgaa ctgctcttgt tgccatgttg ttgtttcttc
    10321 aattgaataa ttgtccagtt gctttttctc gtcaagaagc tgttgatttt atcataatga
    10381 ttaccactag ttcattggat ttcgattcac taaagtccaa gataaccagt tatttacgac
    10441 aaaccgcaat aaaatgaacg aattaaaaac tttatttc
//
LOCUS       DACEZN010000093        10436 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEZN010000093 DACEZN010000000
VERSION     DACEZN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.093"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            90..308
                     /locus_tag="IXH60_001882"
     CDS             90..308
                     /locus_tag="IXH60_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990792.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(597..1109)
                     /locus_tag="IXH60_001883"
     CDS             complement(597..1109)
                     /locus_tag="IXH60_001883"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1990793.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="IXH60_001884"
     CDS             complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="IXH60_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1990794.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3562..3996)
                     /locus_tag="IXH60_001885"
     CDS             complement(3562..3996)
                     /locus_tag="IXH60_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1990795.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4006..4794)
                     /locus_tag="IXH60_001886"
     CDS             complement(4006..4794)
                     /locus_tag="IXH60_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1990796.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4791..5732)
                     /locus_tag="IXH60_001887"
     CDS             complement(4791..5732)
                     /locus_tag="IXH60_001887"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1990797.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5909..6496)
                     /locus_tag="IXH60_001888"
     CDS             complement(5909..6496)
                     /locus_tag="IXH60_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1990798.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6717..8030)
                     /gene="obgE"
                     /locus_tag="IXH60_001889"
     CDS             complement(6717..8030)
                     /gene="obgE"
                     /locus_tag="IXH60_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1990799.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8318..9328
                     /gene="gap"
                     /locus_tag="IXH60_001890"
     CDS             8318..9328
                     /gene="gap"
                     /locus_tag="IXH60_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1990800.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9413..10348)
                     /locus_tag="IXH60_001891"
     CDS             complement(9413..10348)
                     /locus_tag="IXH60_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1990801.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2888 a         2356 c         1809 g         3383 t
ORIGIN      
        1 tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat acgaacaatt
       61 gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt ggcttagtat
      121 taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca gctttacttg
      181 tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag taccgtcaat
      241 ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac agatccaggc
      301 aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac ttttacgggg
      361 gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa agccagtttt
      421 tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg tctttgcttg
      481 tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg acttttgagg
      541 gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa
      601 tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc
      661 aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc
      721 gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct
      781 ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc
      841 acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct
      901 aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata
      961 tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca
     1021 ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga
     1081 atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt
     1141 ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg
     1201 tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa
     1261 aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa
     1321 taatttttca gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat
     1381 tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc
     1441 attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga
     1501 ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt
     1561 tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc
     1621 ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct
     1681 accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag
     1741 accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt
     1801 gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt cttgagtacc
     1861 aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg
     1921 ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg
     1981 aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac
     2041 tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc
     2101 aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga cattttcact
     2161 tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat
     2221 ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga
     2281 tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc
     2341 agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt
     2401 ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc
     2461 ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc
     2521 aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc
     2581 aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa
     2641 tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag
     2701 gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc
     2761 caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac
     2821 tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata
     2881 agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc
     2941 ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa
     3001 ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc
     3061 aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat
     3121 taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc
     3181 actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg
     3241 atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta
     3301 acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg
     3361 gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac
     3421 tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa
     3481 agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg
     3541 accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt
     3601 tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc
     3661 ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt
     3721 tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg
     3781 tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc
     3841 accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc
     3901 aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa
     3961 aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa
     4021 aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac
     4081 ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat
     4141 ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct
     4201 tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc
     4261 tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt
     4321 cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt
     4381 aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat
     4441 cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa
     4501 gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc
     4561 catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc
     4621 ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat
     4681 tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc
     4741 gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct
     4801 tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca
     4861 aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg
     4921 atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga
     4981 gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca
     5041 tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg
     5101 cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc
     5161 gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca
     5221 gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca
     5281 accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat
     5341 tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt
     5401 aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg
     5461 taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt
     5521 ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga
     5581 atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca
     5641 ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca
     5701 cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc
     5761 tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta
     5821 aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa
     5881 cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt
     5941 tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt
     6001 cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag cgagtaattt
     6061 ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa
     6121 tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct
     6181 gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc
     6241 ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg
     6301 tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc
     6361 cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta
     6421 actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga
     6481 ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt
     6541 aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa
     6601 agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc
     6661 acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat
     6721 tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt
     6781 gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt
     6841 tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc
     6901 cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag
     6961 tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac
     7021 aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga
     7081 aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat
     7141 ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga
     7201 cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca
     7261 taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt
     7321 tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag
     7381 cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta
     7441 ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat
     7501 agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc
     7561 aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca
     7621 ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca
     7681 actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc cgcatctcgg
     7741 acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct
     7801 ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc
     7861 aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg
     7921 ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg
     7981 cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc
     8041 tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact
     8101 tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct
     8161 tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt
     8221 gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc
     8281 gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa
     8341 cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat
     8401 tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt
     8461 tgactctact catggtactt atcctggcac agttactgca acagaagact caatcgtagt
     8521 ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa
     8581 agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc
     8641 aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat
     8701 gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc
     8761 agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt
     8821 tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt
     8881 agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat
     8941 cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa
     9001 attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc
     9061 agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt
     9121 tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac
     9181 aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca
     9241 acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg
     9301 cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg
     9361 actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa
     9421 catattggct taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg
     9481 cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat
     9541 agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca
     9601 ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag
     9661 cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca
     9721 tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga
     9781 ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca
     9841 tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta
     9901 tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt
     9961 ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg
    10021 cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt
    10081 cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga
    10141 tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg
    10201 atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag
    10261 cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc
    10321 ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat
    10381 aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt tataac
//
LOCUS       DACEZN010000094        10288 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEZN010000094 DACEZN010000000
VERSION     DACEZN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.094"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(21..251)
                     /locus_tag="IXH60_001892"
     CDS             complement(21..251)
                     /locus_tag="IXH60_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990802.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IXH60_001893"
     CDS             complement(687..1253)
                     /locus_tag="IXH60_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1990803.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IXH60_001894"
     CDS             complement(1253..2209)
                     /locus_tag="IXH60_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1990804.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IXH60_001895"
     CDS             complement(2209..2865)
                     /locus_tag="IXH60_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1990805.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IXH60_001896"
     CDS             3235..5253
                     /locus_tag="IXH60_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1990806.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IXH60_001897"
     CDS             5680..8094
                     /locus_tag="IXH60_001897"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1990807.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IXH60_001898"
     CDS             8442..9995
                     /locus_tag="IXH60_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1990808.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
BASE COUNT         3394 a         1907 c         2045 g         2942 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgt
//
LOCUS       DACEZN010000095        10264 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEZN010000095 DACEZN010000000
VERSION     DACEZN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.095"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1014
                     /locus_tag="IXH60_001899"
     CDS             <1..1014
                     /locus_tag="IXH60_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1990809.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /gene="topB"
                     /locus_tag="IXH60_001900"
     CDS             1025..3229
                     /gene="topB"
                     /locus_tag="IXH60_001900"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1990810.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3470..4063
                     /locus_tag="IXH60_001901"
     CDS             3470..4063
                     /locus_tag="IXH60_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1990811.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="IXH60_001902"
     CDS             4076..4975
                     /locus_tag="IXH60_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1990812.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="IXH60_001903"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="IXH60_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1990813.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="IXH60_001904"
     CDS             5817..6077
                     /locus_tag="IXH60_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990814.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            6522..6728
                     /locus_tag="IXH60_001905"
     CDS             6522..6728
                     /locus_tag="IXH60_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1990815.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            6728..6979
                     /locus_tag="IXH60_001906"
     CDS             6728..6979
                     /locus_tag="IXH60_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990816.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            6995..7411
                     /locus_tag="IXH60_001907"
     CDS             6995..7411
                     /locus_tag="IXH60_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990817.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            7393..7665
                     /locus_tag="IXH60_001908"
     CDS             7393..7665
                     /locus_tag="IXH60_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990818.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            7832..8233
                     /gene="tnpA"
                     /locus_tag="IXH60_001909"
     CDS             7832..8233
                     /gene="tnpA"
                     /locus_tag="IXH60_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1990819.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            8245..9390
                     /gene="tnpB"
                     /locus_tag="IXH60_001910"
     CDS             8245..9390
                     /gene="tnpB"
                     /locus_tag="IXH60_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1990820.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            9628..9891
                     /locus_tag="IXH60_001911"
     CDS             9628..9891
                     /locus_tag="IXH60_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ1990821.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            9885..10235
                     /locus_tag="IXH60_001912"
     CDS             9885..10235
                     /locus_tag="IXH60_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1990822.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
BASE COUNT         3832 a         1677 c         2042 g         2713 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     1141 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa
     6361 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt
     6421 gtccttgtcg cttttcgttg attcattctg gccaacaaat caataaagaa agaactaacg
     6481 aaaggaagta aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt
     6541 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccacat actgtcgaat
     6601 gcgattgtgg agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat
     6661 gcgggaaaaa atataccttg tcttatggcg attatatttt gattgatgag aaaggaaaga
     6721 aacgttgatg aaaacacaac acaaattatt gactgtaaaa gaagtcgttc aaattttggt
     6781 ggacaaagaa gtcatttctg aaaaagtagc agaagcttta cttattccgc ggtactggac
     6841 gacagaaacc cctttagtag aggatgcacc gactatgcta tttgaagacc tagtgaagat
     6901 tgcaggatat gaactgcatt atcaagaaaa cttggaaact tatgaaaacg aatattggtt
     6961 aaccgaatta gagaactaaa aaggagaaaa aaatatggct caaaattact atgacgaatt
     7021 tgtcaaatta cctttggata aaatggcaca aaaaatggag gatatgacat tcctatacaa
     7081 tgagacacgt gttccaaaaa aacattataa ggaaagattg tcggtagctg tggaagaaat
     7141 gattgaatca ggtgtcgaaa tgaatctcat tgcgacgtat tatcgaacac tggaagaact
     7201 gaaaaagcaa aatgcaaaat ggtttttcca agccttacta tgcttggaag taggcgtcaa
     7261 accaagcaca atcaaacctt ctgaatacca agctttagaa ctaacgtatg cgaaatttat
     7321 cgaaacgaaa aaagctaaga ctgtttcaag tgaatggtta gattattttg aaaatatcaa
     7381 taagtatggc gcatgttaca tcatgaagta atcagacgga ttcaaaaaga ctttagttgt
     7441 atggaagaaa tgaagtactt tgttcaaaat gttgaagaac agttatattt ggaaagcgaa
     7501 acgagtcaaa caaaggtcac agtcagccat aatcgtttag atgtgtataa acgtcgtcct
     7561 gaatcattaa aaaaaggagc ggagcttcta cgactagaaa cagaaaaata cgtcatgaat
     7621 gtttacctag aaaaaagtga gttggtcgaa aattttatgg actagccgag aacacccgtg
     7681 attttaatca tgggatgaat cggattcagt tgtgacctag ctttagctag gttaaacaac
     7741 tgacaaatat aaactaaata atagtgaaat gtcttgcaat aattgatttt gtagaaatac
     7801 atatattaaa ataattgcga ggtgtttttg tatgtcagaa attcattatg gacggggtta
     7861 tgtctattct attcaatatc atcttgtttg gtgtgtaaaa tatcgacatg atgttttgta
     7921 tggtcaggta gatattgatg tcaaacagtt tttgaaacaa attgctgatg ataataatat
     7981 aaaaattata gaaatagaat cagataaaga ccatattcat ttactcattg aatgcacccc
     8041 acaacactat attcctagta ttgttaaagt atttaaaggc gcatccgcta gactgctgtt
     8101 taaaaaacat cctgaactta aacaactcct ttggggtgga catctttgga atccaagcta
     8161 ctttgtagct actgttagcg aaaatacaga agaacaaatt agaaaatata tacaaaatca
     8221 gaaaaagaaa tgaggtgaat ccgtatgcta caacataggg cttataaatt tagaatctat
     8281 cctaatcaag aacaagaaat attaatgact aaaactattg gctgttcaag atttgtctat
     8341 aattattttt tgaatttgtg gaatcatgaa tatacgacta ctggaaaagg attatcttat
     8401 aattcttgtt ctgccatgct tccacaaatg aaaagaaatg cagagacaat ttggttaaaa
     8461 gaagttgaca gtattgcgat tcaatcctca ttgaaaaatc tttctgatgc tttttctcgc
     8521 ttctttaaaa agcaaaataa acgaccacaa tttaaaagta aaaaacatcc tatacaaagt
     8581 tatacaacta aaaatgtgaa taatagtatc gaaattagaa acaatttcgt aaaactacct
     8641 aagttaggat ttgttaaatt tgcgaaaagt caagagccca aagggcgaat cttaaatgct
     8701 acgattcgaa aaaattcgag tggtaaattt tttgtttcca tcctgtgtga agaagaaata
     8761 gttgagtttc ctaaatctaa ttccgctatt ggaatcgacc ttggtataac tgattttgca
     8821 atcctttctg atggtcaaaa gattgataac aataaattca cgtcaaaaat ggcaaaaaaa
     8881 ctcaaacgtg aacaacgtaa gttgtcaaaa cgtgcattac tagctaaaaa gaaaggcgtc
     8941 aatctctttg aagctaaaaa ttatcaaaaa caaaaacgaa aagtagcacg actacatgaa
     9001 aaagtaatga atcaacgcaa tgacttttta aacaagttga gcacagagat tatcaaaaac
     9061 cacgatatta tctgtataga agacttaaat acaaaaggta tgttgcgtaa tcataaacta
     9121 gcaaaaagca tttctgatgt gtcttggtct agttttgtga ctaagttaca atacaaagct
     9181 aactggtacg gtcggaaaat catcaaagta gataaatggt ttccatctag tcaaatctgt
     9241 tcaaattgtg gacacaaaga cggcaagaaa tctctagaaa ttcgagaatg gacttgtcct
     9301 gtttgtcatg ctcatcatga ccgagatatc aatgctagta tcaatatttt gaccgaaggt
     9361 ctaagattac atgtcatggg attagcttag atccaatcat caaaaaccgt aggaactacg
     9421 gggatagctt ggtaaataag agaaaccgct gtgagtaaag aaatacgctt gcaagtatgc
     9481 tctattccca agaatctcgt gactttagtc atgagaggtt caaggattca tggaatgtta
     9541 atctaattta atagaaaagg ataattaagt atatacgttt tttcttatgt atgttatact
     9601 atgtatatac agaaagaggt gtctactatg gaaatgttac ctataaaaaa atggggaaat
     9661 agtaatggcc ttagattgcc aaagtatatt atggaatatc ttggaattca tacagaagac
     9721 aaagtgaaga taatacaaga agaagtaaac ggtcagaagc ggctaattat cgaagcggct
     9781 aatatagaaa atgaattaac aattgaacag ttatttgaaa attacactga tgaaagaaat
     9841 catgtgacga ttcaaaattt aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta
     9901 gacaaggcga tattctatta ttgaatacag cacctcgatc tggacacgaa cagacaggta
     9961 aaagacctta tattgtttta agtcatgata ttattgcgga ttatagtaac gttgttacag
    10021 ttgctccaat ttctagtaca actagaaact atccgctata tgtaaatata aactcagaat
    10081 atgaaatgaa gacaacagga aaagtattat tagatcaact aacgacaatt gattatgaag
    10141 ccagagagtg tatttttttg gaaaaagcgc atgatagttt agttgaggag ttattgatga
    10201 aagttagaac agtttttcaa aaagtaagca aataaaatag aaacagcagc atgatcaact
    10261 attc
//
LOCUS       DACEZN010000096        10254 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEZN010000096 DACEZN010000000
VERSION     DACEZN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.096"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1295
                     /gene="uvrC"
                     /locus_tag="IXH60_001913"
     CDS             <1..1295
                     /gene="uvrC"
                     /locus_tag="IXH60_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1990823.1"
                     /translation="YHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQK
                     MEKAAENMEFEKAAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFF
                     VRQGKLIERDVSMFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALL
                     ATKVLQPKRGEKKKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPI
                     RIESFDNSNIMGTNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYS
                     RVIREGLPLPDLILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTD
                     LAVVPLERNSPEFFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKML
                     LKEFKSLKNITAATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            1440..2339
                     /locus_tag="IXH60_001914"
     CDS             1440..2339
                     /locus_tag="IXH60_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990824.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            2332..3594
                     /locus_tag="IXH60_001915"
     CDS             2332..3594
                     /locus_tag="IXH60_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990825.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            3755..5653
                     /locus_tag="IXH60_001916"
     CDS             3755..5653
                     /locus_tag="IXH60_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1990826.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(5983..6702)
                     /locus_tag="IXH60_001917"
     CDS             complement(5983..6702)
                     /locus_tag="IXH60_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1990827.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            7039..7371
                     /locus_tag="IXH60_001918"
     CDS             7039..7371
                     /locus_tag="IXH60_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990828.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            7358..9361
                     /locus_tag="IXH60_001919"
     CDS             7358..9361
                     /locus_tag="IXH60_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1990829.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            9380..9850
                     /locus_tag="IXH60_001920"
     CDS             9380..9850
                     /locus_tag="IXH60_001920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1990830.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            9871..>10254
                     /locus_tag="IXH60_001921"
     CDS             9871..>10254
                     /locus_tag="IXH60_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1990831.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSA"
BASE COUNT         3472 a         1735 c         2187 g         2860 t
ORIGIN      
        1 actatcattt gggacaatgc ctgtgtcctt attatttcga cgtagatccg aaagtctata
       61 cgaaaatcgt tgatgaagtg aaaaactttt taaacggagg acacgaaaaa atcgagtcag
      121 agatccatca gaaaatggaa aaagctgctg aaaacatgga gtttgaaaaa gctgcagaat
      181 accgagacca gatccgtgcc attgagacaa tcatgactcg ccaaaaaatg acaaatactg
      241 atttactgga tcgagatgta tttggttatg cgattgataa aggatggatg tgtgtccaag
      301 tgtttttcgt aagacaagga aagctaattg aacgagatgt ttcaatgttt cctttctata
      361 acgaagcaga agaagatttc ttgacttacc tcggtcagtt ttatcaggaa aatgagcatt
      421 tcattcctaa agaagtcttg attcctgata cgatcgacaa gcccagtgta gaagctttgt
      481 tagctacgaa agtgctgcaa ccaaaacgag gagaaaagaa aaaattagtt gaattggcga
      541 gtaagaatgc ctcagttgct ttgaatgaac ggtttgatct gattgccaga aaacaggaac
      601 ggacgatcgg agcagttgaa cgtttaggaa acgcgatgaa tatcccgacc ccaatacgta
      661 tcgagtcttt tgataactct aatatcatgg ggacgaatcc tgtggctgcg atggtcgtat
      721 tcattgatgg aaaaccggca agaaaagaat atcggaaata taaaataaaa acagtcaacg
      781 gaccggatga ttatgcatca atgagagaag tcatctatcg cagatattcg cgagtgattc
      841 gcgaagggtt gcctttacct gatttgatct tgatcgatgg aggaaaagga caagtagatg
      901 tggcaaaaga tgtcttagaa aatcaattag gaatcgatat tcctattgct ggcatggtaa
      961 aaaacgataa acataaaaca aatgagttgc tattcggtac agatttggct gtagtaccgc
     1021 ttgagcgaaa ttcaccagag tttttcttac tccagcgaat ccaagatgaa gtccatcgat
     1081 tcgcaatcac tttccatcgg cagctacgca gtaaaaacag ttttgcttcc cgattagatg
     1141 aaattgaagg gttaggacca aaacgaaaaa aaatgctgct taaagagttc aaatcgttga
     1201 aaaatatcac agcagccacg acagaagaat tacaagaaat cggactgccg aaaaatgtcg
     1261 cacaaaacgt atttgataaa ttacatcagg aatgaggaaa gggcacttta caaagaaagt
     1321 aaagtgtcct atcattttat gtacaacaaa aagagcgttt acgttttgtt ggtaaataat
     1381 aaatcgctta cgtctggtgt ctaaggatat atgtgatttc ttgaagaagg agtgaagaaa
     1441 tgttagaagt caaggatctt gttaaaactt ttgggaacta cacagctgtc gatcatgtct
     1501 ctttccaaat ccccgatgga aaaatcatgg ggctaattgg ccaaaacggg gctgggaaga
     1561 caacgacttt tcgtttgatc ttggattttt tgacacaaga ccaaggagaa gtgttatgga
     1621 atggccatca tcttggggag aaagattatg atgtcatcgg ttatcttccg gaggaacggg
     1681 gactttatcc gaaagtaacg attcaagacc aactgcttta ttttgcagaa cttagaggga
     1741 aatcaagaaa agagattgaa ccaaaaatcg acttttggat ggaaaaattt caggtaaaag
     1801 ggaaaaagac ggataaagta aaatctttgt ctaaaggaaa tcagcaaaaa gtccagctga
     1861 tcgccacgtt aatccatgaa cctaaattga tcattttgga tgagccattc agcgggttag
     1921 atccggtaaa tgctgaattg ctaaaagatg gaatattgga attgaaaaaa catggttcat
     1981 gtgtcatttt ttctagccac aatatggata atgtagaaaa aatctgcgat catttgatca
     2041 tgttgcgaaa cggaaaaatg gttttgaatg gaaaagtaca tgagattcgt gaatcatttg
     2101 gccgtacgaa gttattcttg gaatctggtt taagccaaca ggaagtagaa gaaatcgaag
     2161 gcgtgaaaaa agtgatcctt catgaagaca atacgttaga gattacctta gaagatcctg
     2221 aagctgggaa agaaatcttt actcgtgcga ctcagttcgg ctacattcct atgtttaacc
     2281 agcaaccgcc aactttggaa gagattttca aattgaaagc aggtgaaatc gatgcgtaag
     2341 ttttggatta ttaccaaaga tgtctattta aaaaatgtta agtcgatttc tttcttgatc
     2401 atgatattag tcccttttat catgctgggt atcatttatg tagcaggaaa ctttgctcaa
     2461 caaaatagtg atgtcaataa aatcggcgta ttggctgata atgagcaaat cagtcagcaa
     2521 atgagccaag tcaaaacaga tgattttgag ttcaaagcag ttgattctga agaaaaagca
     2581 caaaaagagc tttcggaaga aaaaatcgat gcgtatttag tagtctcaac tgagaatgat
     2641 aagataaatg ggatgcttta tagcgaaaat tcattaggac aaagtacaca actactgata
     2701 caacaacaac tgactggctt gcaatcgatg cttcgagcga gtcaattagg catttcacca
     2761 gaagaagtag ctagcttgag ccaacaagca agtttgactc gacaaaaagt caattttgat
     2821 tcaaaaggaa aaatgacagt cggagaagat aactcgacag tccaatacgt cgtaagttat
     2881 gtagcaacga ttgtcttgtt cattattatt ttgacgtatg cgcagatcat tgcacaagag
     2941 atcgcttctg aaaaaggcac gcggatcatg gaagtgatcc tgtccagtac gactgcacaa
     3001 aagcattttt acggaaaatt aacaggtgtt ttacttgttg cattaacgca aatggcatta
     3061 tacggtatta tttttggtat aggatacagt caattcaaag atatggaaat ggtcaaaaat
     3121 atattagacg gtatctcatt ggataacttg attggaccat tcttgtggta ttctcttcta
     3181 ttcatgatct ttggcatttt gattttttct gttttagcag ctttatgtgg ctctttggtc
     3241 aacaaagcag aagatacagc aaaagcaatt ttgccagtga cttatctatc tctaggcgga
     3301 tacatgcttg gattgatact aggagcttca gatccaaaca atatcgttat tcggatcact
     3361 tcgtatattc cattcttatc ttcatatatt atgccgattc gcttagcaaa tgagacagtc
     3421 ggtatgggtg gagcattgat ttccttgttg gtacttgtag ccgtaacatt cttattgatg
     3481 atcttgtctg caaacatgta caaatcgaat gtactcgttt atagcgaagg cggattatgg
     3541 agttcattga aacaatcgat ttcaattatg aggaatgaac gaaaaaaagc ataaaacaat
     3601 cctaaaatac agaaggaagc tggacatcta ttgttcccag cttctttttg tatagaccta
     3661 ttttatttat tttactatat tggatatata ttcattgaaa atagtgtata acaactttat
     3721 aatattgagt ataccaattt ttggaggcgt tttaatggac cgttatttag atttactttc
     3781 agaaaaattc ccctcaacgg aggatgtcat cacggaaatc atcaatctcg aagcaatttt
     3841 gcagcttcca aaaggaacag agttattttt gagtgatata catggcgaat ttcctgcttt
     3901 tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa aagatcaaag accttttttc
     3961 tgaacaaatg acggaaacgg atatcagcca attcactatt tttacggctt atccagaata
     4021 tgcattaacg actgattggt acaaagaaca agataaatcg cagctgatcc atcatttgat
     4081 cgatctttta cggtttacga cagtcaaata tacccggtca aaagttcgaa aaggattgcc
     4141 gaaagaatat agctatatca ttgaggaact cttgtatatc gatgaccgta tcaatggaaa
     4201 aaaagactat gtaaaaaaaa tcattgaaca acttgttttg atgaaggaag aagaaaaatt
     4261 tttaacgaaa cttgcacaga cgatccaaaa atctgtcatc gatcatttgc atatcgttgg
     4321 agatattttt gaccgaggaa ctcaagcagc caaagtgatg gatcgaatca tggatttttc
     4381 ttctgtagat atccaatggg gaaatcatga tattttgtgg attggtgcgt attgcggctc
     4441 tgaggcatgt ttactgacac ttttacggat tgcagcacgc tataattatt tatttgaatt
     4501 agagaaagaa tatgggttga atttacgtcc attgttttct tttgctcatc aaacttatca
     4561 gaagaatcca gtatttgcgc ctaaaaacag taaaaatcta tccgaaagag agcaagaaga
     4621 attagaaaaa atccatcagg cactttctat ccttcaattc aaatcagaac atcagttatt
     4681 ggatcgtcga cctgaattta aaatggacga tcgtaaattg ctggaaaaaa tcgactatga
     4741 agcatcaacg attgatttag aaggtcagct ctatgagttg aaaggaactt gttttcaaac
     4801 gatccagcca gatgatccaa aaaaattctt ggaagaggaa caaaaagtga ttgatagttt
     4861 gatgtattca ttccagcact ctttgagaat gcaaaaacat atgacgtttt tgatcgaaaa
     4921 agggggaatg tatctcacga acaatcagca tgtgctgttt catggctgta tacctgtaga
     4981 cgagaaggga cagctcttag catttgaatt agaccaatct tatcgcggaa aagaactgct
     5041 gggctttttt gaaaaacaga taactgaaag cgcaaaagat ctgactgcaa aagaagattt
     5101 agcaaccgat ttgatttggt atgcgtggag tgggccattt tcgcctttat tcggcaagag
     5161 taaaatggcg acttttgaac gttattttct cacagagcct catacgcacg tcgagaaaag
     5221 caatccttat tatcacttgc gagatgaaga gtggttcagt gaaaaaattt tagcagaatt
     5281 cgacacaaaa gaagaagggt cagcaatcat taatgggcac acgccagtaa aagtaaaaaa
     5341 aggggagtca ccgatcaaag caaatggaaa agtatttgtc attgacggag gtttatccaa
     5401 agcttatcaa aaaacgacag gaattgccgg ttattcacta ttatgcaatt cttatggttt
     5461 ccaaatcgtg acacattatc cgtttttgcc tattgaacaa caatttgcaa aaaaatccga
     5521 ccaaacaaat gtgaaaaaag taatcgaaca gcaactacca cgcaaactca atcgtgatac
     5581 aacaaaaggt atggaactgc agcagcagat tacccagctc aaaaggctgt tggactatcg
     5641 aaaggacgat tgagaagaat tccagtgaac attgttccat agactggaga ctgagacaaa
     5701 agtcgtgtcg cagtctcttt ttccggataa acggtgttct tctgccaact cctcggtcac
     5761 aagtcacact attcaacaaa catgaaaagc tgttttttga aattgctcct tgtgactcag
     5821 agttgaacgg caggctcacg acctcttggt ataaacggtg ttcaaaagcc agtttctcgg
     5881 tattaagcca aatttatgca aaaatgtgaa aagccatttt cacaaaattt ttcttaatac
     5941 tcgaaactaa acagcttttt cacaaccccc tcagaaagtc tattaaaatt cattcacaaa
     6001 gaaatcgaat aagcgctgtt cagatggatg tacacggtgg gtaagttccg gatgccattg
     6061 aacaccgagg attttatgca tatcatcttt gctttgaacc gcttcaacga gtccatcgtt
     6121 agaaaaagcg atggctttta ataaaggtga aagatctttg actgcctgat gatggtaaga
     6181 attcacaaaa tatggatcgg ggagaaggtt gcttaacaaa ctgtctgaaa caatcttgac
     6241 ttcgtgagtc gtaaattgcg gggcagtggg ctgttgttca tgttttactg accactttgg
     6301 atataatgaa agatcttgat aaagtgtacc ttccaaaacg acattcaata gttggagtcc
     6361 ccggcagact gcaaaaattg gttttctttg tttcaatgct tccaagatca gtgcctgttc
     6421 aaattggtct cgttgaatat ttgtttccgt tagctttgga tgaggatctt ccatgtaaag
     6481 ttgtggactg acatcttgac cacctgcaag aataagtttg tcgatttgtc caatcagttg
     6541 aggagctgaa tctggagaca tgactggcag aataagaggc aatccttgag catctaaaac
     6601 tgcatccacg aatccttgcg gtgtatagct cacttgattt ccttggaaag tatctgttgc
     6661 tcgaatcagt tgatttccgg caatgccgat gattggtttc atttgtattc cccctaaagt
     6721 agacttattg taacaaggat ttaaaaaaaa ttaaactatg taagacttgc ccctatactt
     6781 atctgttctt gctgtgttac tatctgtaaa gagaaatttc gtgtgaaatt catgacgata
     6841 gttgaaaata ttgcttaatt tgttataata aaaagtaagc agaaataaaa aggttgggcg
     6901 taagcttcaa ggatctagtt cttcaagggg tgagaaggac attttattga aggggcctgc
     6961 ctatactaag ttatctttag aggtacatac tttcaatgaa aaaagaaagc atgtacctct
     7021 tttttaaatc gaaaggaagt gaagcaagtg ttagaagctt tggaacaaat ccgaaaagca
     7081 gaagaagaaa atgaaaagcg tgctcaaaca ctgcggcagc agctgaagat ctatgcaaca
     7141 gaaaaagagc aggaattgga aacgatccaa acaaactatc gacaacaact aaaacagttg
     7201 gtggaagaaa aagaacaatt tgaaatacaa gaattagaaa aagaaaaaat gaaattgaaa
     7261 gcagaagcag acaaggtgca agcaactttg aaagatcaat atcaaaagca tcagagtcaa
     7321 gcgatcggtg cgatcattga aagggtgaaa gaaacttatg gccgtcacta agatggaaaa
     7381 agtgaccctc atttcagata aaagaaatca ggagaggatt ttacaagcag tacaaggaat
     7441 ccaaaacgtt gaaatccggg atctgtatca aaatagaaca aacaaccagt gggtggagaa
     7501 gtattttcca gataccacga ttattgataa agaagcaaaa ttaagcgctt taggtaatcg
     7561 gttgaaagac atccaagaag ctatccaatt catacaacac cacggcgata gccgtcaaaa
     7621 gaaactccat ttgaaacgtc aagaactttc cttacaagat ttggaagcca attattccga
     7681 agaaaatttc ataaataaat tgaaagaagt ccttgattta aaagaaaagt gggaacaact
     7741 aactgaagaa cgtgatcgtt tagaagaaga ggaagaatgg cttcttcatt ggcaacagct
     7801 ggatattgct ccaagtatgt atgcaagtaa gacgaccatt tttgagatgg ggactgtgag
     7861 tgcagcaaat tttgaaagtt tcaaggaaga actgagtaag cttgatgacg tttatatgga
     7921 agaaatagat tatacgccaa aacaagttta tctttcgtat gttgctttaa aaaaatcagt
     7981 tgctgttata gaagaaattg ctgggaaata cgggttcaat aaagaaacgt atccttatga
     8041 acaaaatcct aaagaaaaac tgcaagagac caagcagcaa ttatccattg ttcacgagca
     8101 gcaaaagaag ataacgacag cgttaggtca gtgcagtggc tatatccaag aatttgaatg
     8161 ggtagaagaa gtcacacttg ccgttgcaga acgagaaaaa ataaaagaac ggtttatcca
     8221 tgcgacttat ctgatcgttt tgcaaggttg ggtagatgca gaagaaaaac agaatttgct
     8281 gcatgtgtta aacgaaaccg tatcagaaga agatatatac gtcagtttcg aaacaccaaa
     8341 ctcagaagag attcagcagg aagtccctac taaattgaaa aaccatccgc ttgtcgaacc
     8401 ttttgaattg ctgactgaaa tgtacagtct gccaaaatat gaagaagtag atccaacacc
     8461 ttggatgaca ccattttatc ttgtcttctt cgggatgatg gtcgcagatg ttggttatgg
     8521 tttgctgatg ctcattggga caatactagc tcaaaaacta cttgttctac cacgcgggat
     8581 gaaacgtttc gtaaaattct ttgagattct ctcgatacca tcgatcatct ggggattgat
     8641 ctacagttca tttttcggac agtcattacc aaaagaactg tttggcatcc gcttgccttt
     8701 cccaatccta tctacgacgg atgacgtcaa tacgattttg attttatccg tgattttcgg
     8761 tctgatacag attttggttg gattatttgt ggcagcaaag gaaaatatca ggagaaaaga
     8821 ttacttgagt gctgtcagtg atggatttgc ctggcaaggg atcctaattg ggatcgttat
     8881 tgcgttggtt ggtgccatgt tgataaaaaa tacagcattt gtttaccttg gcgctgggct
     8941 atcgatcatt gctgccttat gtatcttgat cgttccgatt atccaaacgc cgtctaaagt
     9001 caaaggtgct gccaaaggtg cgtataatct ttacggactg accggctata tcggtgattt
     9061 agtcagttat acacgtttga tggcattggg tatttcaggc ggaagtattg gagcggcatt
     9121 caatatgcta gttgctttca tgccgccagc tgctcgattc agtgtaggga tccttttgat
     9181 catcgcttta cacgcactga atatgttttt gacactgcta agtgcctatg tccatggcgc
     9241 acgattgcag tatgtcgaat tttttgggaa gttttataca ggaggcggac gtgcctttga
     9301 tccgctgaaa acggcggaaa aatacgtcaa catcaatcac aagaaaaaaa acaataaata
     9361 attggaggat ttttatataa tgatggatta cttaattagt caaaatggtg gaatggtatt
     9421 tgcggtatta gcgatggcaa cagcgacgat tttttcaggg atcggttcag ctaaaggtgt
     9481 tggaatgaca ggtgaggcag cagcagcatt gacaacaagt caacccgaaa aattcgggca
     9541 ggctctgatt ctgcaattgc ttccaggcac gcaaggtttg tatggattcg ttattgcctt
     9601 tttgattttt atcaacttag gaaatgacat gtcagttgta caaggactga actttctagg
     9661 tgcttctctg ccgattgctt ttacaggctt gttttcaggt attgcccaag gaaaagtcgc
     9721 agcggcaggt attcaaattt tagcgaaaaa acctgaacat gctacaaaag gaatcatttt
     9781 tgctgcgatg gttgaaacat acgcgatttt aggtttcgtt atttccttcc tattagtgtt
     9841 gaatgcctaa gcataaagga ggaaaagcag atgaacgcca ttgaaaaaat catctctcag
     9901 atgaacgaag cagcagagca ggaaagagcg gcgttagaac aagaagagcg aatgaagatc
     9961 gatcaaaact ttgaacaaaa acgtacgcaa gttgaaacag aacatcaaaa acaaaaagaa
    10021 aaacaaatcg aattactcga aaaaaaatac cgtcagctaa ggaatcgcca gcaagtagaa
    10081 gtacgacaag aaaatttgaa tgcgaagcag gaatttttaa gacgattgtt tgcagatgcg
    10141 gtaactgaaa tggaaaattg ggatgaatct gaacaaatcc agtttatcaa aaatgcattg
    10201 tattcattac ctttgacagg aaaagtagca ttcattgctg gagaaaaatc agca
//
LOCUS       DACEZN010000097        10149 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEZN010000097 DACEZN010000000
VERSION     DACEZN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.097"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(423..1358)
                     /gene="whiA"
                     /locus_tag="IXH60_001922"
     CDS             complement(423..1358)
                     /gene="whiA"
                     /locus_tag="IXH60_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1990832.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1392..2390)
                     /locus_tag="IXH60_001923"
     CDS             complement(1392..2390)
                     /locus_tag="IXH60_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1990833.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2387..3271)
                     /gene="rapZ"
                     /locus_tag="IXH60_001924"
     CDS             complement(2387..3271)
                     /gene="rapZ"
                     /locus_tag="IXH60_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1990834.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3406..4137)
                     /locus_tag="IXH60_001925"
     CDS             complement(3406..4137)
                     /locus_tag="IXH60_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1990835.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(4141..5406)
                     /locus_tag="IXH60_001926"
     CDS             complement(4141..5406)
                     /locus_tag="IXH60_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1990836.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(5669..8485)
                     /gene="uvrA"
                     /locus_tag="IXH60_001927"
     CDS             complement(5669..8485)
                     /gene="uvrA"
                     /locus_tag="IXH60_001927"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1990837.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(8495..>10149)
                     /gene="uvrB"
                     /locus_tag="IXH60_001928"
     CDS             complement(8495..>10149)
                     /gene="uvrB"
                     /locus_tag="IXH60_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1990838.1"
                     /translation="EKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFE
                     YQKQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERA
                     LRVEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQ
                     RLTVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLD
                     FFPEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKH
                     VNQIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVE
                     KDQRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVG
                     INLLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDS
                     MRLAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEM
                     TKEEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
BASE COUNT         2804 a         2278 c         1704 g         3363 t
ORIGIN      
        1 cttgttaaaa cttctacatt aattacacct ggttgttttc gactagctaa tccaatgaac
       61 ttatcaacca tccttatcca ccaaccacga gaatcttgac cagaagtaag aaaaacagtt
      121 ctcaaggttg atcgagcagt tccttccagt ttttaattaa tctccagtta tagataaata
      181 cttgatccta tctatatcag gacttctata ttaagcaaaa gtttatgtat aaaactaaaa
      241 tcttcattca gttgtctccc cgctttttat acgattgtat taaaaaacaa tagaagcctt
      301 tcaacattat gttactccag taaagttgga attatagttt gacggaagaa aagcaagtat
      361 agcttttaaa aaaaactgat acattcgcat ctcctgcttc atgtatcagt tttttctttg
      421 gattaaactg cttcttcacg cagtttttcc gcaaattcat ttattttgcg aattcggtga
      481 ttaattcccg attttgatat tgcacctgag ggaatcattt cccctaattc tttcaagctg
      541 acctctggat gttgaagccg aagttctgcg atttcctgaa gtttttcagg aagggcatgt
      601 agaccgacct ttgcttcgat caattcgatg ttttcgattt gtttagaagc cgcatcaatc
      661 gtcttgttca gatttgctgt ctcacaattg actagacgat tgaccgaatt tctcatatcc
      721 cgtacgattc gaacatcttc aaatcggagc attgagttgg ttgcaccaat caatgttaag
      781 aaatcagcaa ttttttctgc tccttttaaa tacgaaatat agccattgcg ccgttcaagc
      841 gttcgagcat gtagaccgta ataatttaac atatcgcaaa tgtcttggtt atgctgttca
      901 taaatagaaa atatttccaa atgataacga ctcgtttccg gattattgac tgaacctgag
      961 gccataaaag caccacgtaa gtaagaacgc attttttgcg aatttcccat aatctcgtcc
     1021 gaaacgtgtc cattgaacat cataccatcc atgatatcaa gatccatcaa cacatctttt
     1081 gtgccttgct tcaaccggac gatatagaca ttgttctttt ttaatttcat ttttttacga
     1141 acgagtagct cacttcgtac ctgataatga tctttcaaaa gagaatagat acgtctggca
     1201 attgcagcat tttctgtttg gacgttcaaa ataaactgct ggttcacaat acctaaagag
     1261 ccgttcattc gcagcaaagc agctaattcc gctttcgcat gttctcgatg aacttccaaa
     1321 ccagttagtt ctttcttcac atctgcagca aatgacatcc tattctcctc ctttctccaa
     1381 caataaattg cttaatattt tgaaccgaaa actaaacgga acaattcttc tactactttg
     1441 tctccatcgt ggaagacacc accatctttc aattctaaaa agtcggtgga aatcactcga
     1501 catccttcat ttctcaatcc gttgaagtcg tgtgcgactt gtactagata ctcatcatag
     1561 atctctgggt ccatataatc ttcaggcact ttttccgtat tgaccaatac tgtatcgata
     1621 aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat cggcatctgt gaaatgttcc
     1681 gtttcccctt tttgggtcat gatattacag atatagacgg tttcagcagt tgtcttgagc
     1741 attgcttcgc cgatttctgg tatcactaaa tttggaagga tactggtgaa taaacttccg
     1801 ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa cgacttttcg agccgctttg
     1861 gcttcacttt tatcatttgt gttgcagaca tacacacggt caatggtttt acggttgatc
     1921 gcgattttag attctcctac tgcagtcgtt ccgtctttaa aaacagcatg caaggtcaaa
     1981 ggagtttctg aagaaggata aacatgtcca tctacatgca tcatttttgc taataattga
     2041 atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg caatgatcag attaccgatc
     2101 gtatgattgg ctaaaaactg atcttccttt ttgaaacggt attggaaaat atcttcatag
     2161 aattgtggca tatccgataa cgccactaag acatttctca agtcaccagg aggagccata
     2221 gcgatcgatt ctctgattgc accgctgctt ccaccatcat ctgcaacagt cactactgct
     2281 gtgatatctg ctccttgatt tcgtaaactt ttcaggataa ctggcagacc agtgccaccg
     2341 ccgaccacta cgatctttgg ttttcttatc cggtaggttt tcattttcac gaccggttca
     2401 ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt gactttatag tttttcccta
     2461 aagcatttgc caaacgttca gtcaatgcca cagaacggtg ttgaccgcct gtacagccga
     2521 ttgcaactgt caagctgctt tttccttctt taatgtatcc aggcaataca gtttccagaa
     2581 gatccaaaaa tttatggtag aatgattgcg tttcatcaaa ggacatcaca taatcataga
     2641 ctggctgatc ttttccggtc aatggtctta gttccgggat ataatgaggg ttgggcaaaa
     2701 aacgcacatc catcacaata tccgaatcga tcggcaaccc gtatttaaaa ccaaatgata
     2761 caaattcgat ccgaaatcct tggtctccct ttgatttgaa tgactcattg attttttcac
     2821 gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac ggaagcttgg gctttcaagt
     2881 cgtctagtat cgcccgttct ttacgtatcc cttcagtgat caaaccgtcc atcgccattg
     2941 gatgtgctcg tcttgtttct ttgtagcgag ccactagttc ttcgtctgaa gcatctaaaa
     3001 ataagataga agtgtcgatg aagttcgtat tttcgatttc gatcagcatg ttttggattt
     3061 cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc catttttgtg actttacctg
     3121 attctttgat caattcccag aattttggaa tcaatcgagg cggcatatta tcgatacaaa
     3181 aatagcccat atcttcaaaa ctttggatag ctactgtttt ccctgcacca ctcatacctg
     3241 ttatgatgac taattgcaaa ttatctgtca tatttacgaa cccctctcac attgtgcata
     3301 ataaaattat atcatcccgg gcgtatcatt accagttttc tttttctttt acaaaaagat
     3361 ttggaccaaa tatcttttag gatatttgcc ccaaatccaa tttttttact tttccgatgc
     3421 tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg atcgttcgat ccgctaaaat
     3481 agattgagca tcgatcactg ggtaatgatt atcttcagca aattcatgca tcaaagataa
     3541 ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc tctaccgctt cctctaagat
     3601 ttcgtaatag agctcttgat tcaaataatc cgattccttg atctcatgat agatcaggta
     3661 attgattttt tcttgaagcg cagtatcagg aatcactgtt tcgaaaccaa gatttttgac
     3721 ttcgcgttca tagattccag ctttcatact tccttctgtt cctagaatcg ctactctccc
     3781 tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt ggcatatgca agataggaat
     3841 atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg gtgttacaag tcaacacaat
     3901 gaagtttggt cgcaataaat tttgtttctc aatatcatcc agcagaaacg gcattggatt
     3961 ttcttctgat cgatctaaaa tataagctgt acgatcagga actgttgcat gattgaataa
     4021 gacataattc aaatattctt gatcttttgt agctttcgtt cggtggttga tcaagcgaac
     4081 aaaactttct gtcgccatcg tgcccattcc gcctaaaata ctgaaaaaat tctccatgaa
     4141 ctagccttct ttcacatgaa aatacttttt gaatcttgga atatagttat ggaacatata
     4201 tttcatcaac aaccagcgct tgattgatcg gtctttttca taaaatacag tagtaccgta
     4261 acgattttct ttgatcattt gctcagctag ttttttgtct tcattttccc gagcatattc
     4321 taaaaagatt tttttgggca cacccatcca taatcttgct ttatctgatt gattcgttcc
     4381 ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct tctgtgacaa aacgagccag
     4441 attcaagccg ttcaatgtca caaagaaact gctacgacct tgtctcaaat tgatttcaaa
     4501 taatttatat tctccatctc taggatcata cttcatatcg aaattggcaa agcctgtata
     4561 ttcgattttt tccaaaaatg ccttaatggt ttgatagatt ttttcgttat aatctggcat
     4621 gatcacaacg taattaccaa tcgatgctgg tgtaggatct tctaatagtg gatgaccaag
     4681 gcacatcatc cgaacttgat gatcttcatc tacatacgca tttagcacac gcatattact
     4741 atcatcaccc gggataaaat cttgtacaat catttcactt ttataaccgg cttcgtatat
     4801 ccggcctaaa attaaatcga actcttctct agtctctaaa ataaaagctt tttttcttcc
     4861 ttcaaattgg acagaaagat actccacact gtttgcaggt ttcaaagcaa ctgggaattc
     4921 aaaaggcaat tcctgttcca aatgtccatt tactaacatt tcttctctga cgatcaatgt
     4981 tttaggatac ggaagatcat attcttcaca aacttcataa aagcttactt tattgatcag
     5041 cctttcaaat aaagaataat caatatatgg acagataaaa tattctgaca gctcttgttt
     5101 atgttgggag atcaattctg catatccatc accacaagca atcaaaagat attttttact
     5161 cttatcagaa tatctttctt ttgccaaacg aagcatcgtc tcgataaata cagggtcttt
     5221 atcaaatcct ggaataactt ctacattgac aattttactg tagcgggtag gggctaactg
     5281 atccgaagca tatgcttggc agattttgcc gtatgcttca ttaaaagaac gtgccatacc
     5341 ataaacgttc atgtcgcttc ctaataatat agggataaat tcttcatttt caatactgtt
     5401 catcattatc tctcacttac tcataatctc ttgtttttct tttccaacta cacaaggaat
     5461 atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc cgattgctca attctttttt
     5521 gaacggattg aacttatagg aagtttttat ctataccaat cagtgtagaa agaactacca
     5581 tgtaatgatt ctagaacact gatattaata aaacaagcaa aaattcattt ttttatgttt
     5641 ttttaactat ctcagctatc tatcctcttt ataccattat ttttttcaaa tagtgacctg
     5701 tataactttc ttttacgtta atgatttctt ctggtgttcc agtcgcaagg atcgttcctc
     5761 caccttctcc accttctggt cctaaatcaa tgatataatc tgctgttttg attacatcta
     5821 gattgtgttc aatcactaaa actgtgttac cagcatctac taatctttgt aatacatgca
     5881 acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg ttcatctagt atatagaaat
     5941 tctttccatt agagattttg tgcaattcac tggcaagttt catccgttgt gcctcaccac
     6001 cggacaatgt cgttgctggt tgccccatcg tcacataacc taagccaaca tcaacaatcg
     6061 tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa ttctacagca tcctctactg
     6121 tcatttccaa aatatcagca atgctttttc ctttgtaatg cacttctaat gtttcagaat
     6181 tatatcgttt accatgacat acttcacaag gaacatagac atcaggcaaa aagtgcattt
     6241 cgatcttaat aattccatcc ccgcgacaag cttcacaacg accgcctttt acgttgaaac
     6301 taaaacgccc tttcttataa ccccgcattt ttgcctcgtt cgtttgagca aataacccac
     6361 ggatatcatc aaatacactt gtataagtcg ctggattact tctcggcgtc cggccgattg
     6421 ggctttgatc gatatcgatg atcttttcga tactttcgta gccggaaatt gtcttgaatt
     6481 tacctggctt agcagaattc ttatttaatt tttgcgctaa cgatttcttt aagatactat
     6541 tgaccaatgt actctttcca gaaccagaca cacctgtgac tgctacaaat tctcctaatg
     6601 gaaattcaac actaatattt ttcagattgt tttctgaagc accagtgatc ttgattgctt
     6661 tcccattacc tttacggcgt tcttttggta cagggattga ttttttccct gaaagatatt
     6721 tccctgtcaa agaattttca tcattagcta cttcttcagg agtgcctgca gccacgattt
     6781 gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa ataatctgct gcccacatcg
     6841 tatcttcatc atgttcgact acaacgaggg tgttgccaag atcacgcatt ttctttaatg
     6901 agccaatcag tcgatcgttg tcgcgttgat gcaatccgat cgatggttca tctaaaatat
     6961 acaaaactcc agaaagattc gatccaattt gtgtagccaa tctgattcgc tgcgcttctc
     7021 ccccagataa tgttccggaa gcgcgactca aagttagata gtctagtccc acattttcca
     7081 agaatgaaag tctgtcattt acttctttta aaatcggttt ggcaattact ttctcttgtt
     7141 cagataacgt taagtttgag aagaaatcta ctgctttttg aatagacaac tcatttgttt
     7201 gcccgatatt cgttccattg atttgaacag atagagcttg gggattcaat cgataacctt
     7261 tacatgtctg acaagttaat tctgtcatat aaagacgcat ttgctcacgc gtgaaatcac
     7321 tgttcgtatc atgataacga cgctcgatat tcttcaatac cccttcgaaa gtcgtttcta
     7381 catcgcgaac gccaccaaaa tcattttcat aatggaaatg gaaaagatca tctgtcccat
     7441 tcaagatgat ctcctgatgc tcttccggta attcttcgaa tggtgtatct aaatcgatac
     7501 cgaaactttc acaagcctgc gctaacattt gaggataata ctgagagctg attggattcc
     7561 atggaacgat agcaccttga ctaagtgtct tcgttctatc tggaatcact aaatcttgat
     7621 cgacttctaa tttgacacct aaaccatcac agtctggaca agctccaaaa ggcgcattga
     7681 atgagaacag tcttggttct agttcgccaa cagtaaatcc gcaataagga caggcatagt
     7741 gttcgctaaa caacatttct tcttcaccga tcacgtcaac cagagcatag ccgtctgcta
     7801 atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg aatcccttct ttcacaatga
     7861 tccggtcgat gacgatcgcg atatcatgtt ttttgttttt ctccagctca ggcacttcac
     7921 ttaaatcata gatctcacca tcgactcgaa cgcggacgta tccctctttt tggattcgtt
     7981 cgaatatttt tttatgttgt ccttttttct ttaccacgat cggtgccaac acttggatct
     8041 ttgtgcgctc aggcaaagcc attacttgat ctgccatttg atctactgat tgactggtaa
     8101 tctcgatatg atcgttggga caaatcggat gtcctacacg tgcaaacaac aagcgtaaat
     8161 agtcattgat ttccgtcaca gtacctactg tcgaccgagg atttttactt gttgttttct
     8221 gatcaataga aatcgccgga ctcaatccgt cgatgctatc tacgtccggc ttatccattt
     8281 gtcctaaaaa ctgacgagca taagcagaaa ggctctctac gtatctccgc tgcccttcag
     8341 cgtacagcgt atcaaatgct agggaacttt ttccagaacc ggacaaacct gtaacgacca
     8401 cgaatttatc tcgtggaatc gttacatcta tatttttcaa gttatgggca cgtgcaccat
     8461 gaatcgtaat tttatcgtta gccatgttgt cctcctactt tgaagctttt aattctaaaa
     8521 tcatatctcg cacattcgcg gctgtttcaa aatccaaagc ttttgccgca tctttcattt
     8581 ctttttctaa tttcatcaat aacgtgtctt tttcttcctt cgtcatttct tcgtacgaga
     8641 cttgtaccgc ttcttttgta tcatcttccg attctttcgt aatagaaatc aaatctcgga
     8701 tttctttaat gatcgttttt ggaacgatcc catgttcttc attgtacttc tgctgaatcg
     8761 tacgacgtct tgacgtttca tccatcgcta gacgcataga atctgttact ttgtctgcgt
     8821 acatgattac tttaccttct tcattccgag cagctcgtcc aatcgtttgg actaaggaac
     8881 gttcactacg taaaaagcct tctttatccg catccaagat agcaacaagg gaaacttcag
     8941 gtacgtctaa tccttcacga agcaaattga ttccaactaa cacatcaaat tcacctaatc
     9001 gaagatctcg aatgatttct gttcgttcca gcgttttgat atcactatgc aaatatttga
     9061 ctttgatccc taattctttg aaataatctg tgaggtcttc tgccattttt ttcgtcaatg
     9121 tcgttacaaa cacccgctgg tctttttcga ctcgttcatt tatttcgcct actagatcat
     9181 cgatttgtcc catgattgga cgaacttcga tcactgggtc aagcagccct gtcggacgga
     9241 tgatttgttg gataacggta tctgtttgtt catgttcata tggtcctggc gtcgcagaga
     9301 cataaatgat ctgattgaca tgtttttcaa actcttctaa ccgaagtggc cgattgtcta
     9361 aagcagatgg caaacggaaa ccgtaatcta ctagcatctg ttttctcgct cggtcaccat
     9421 tatacattcc tctgatttga ggcatggtta cgtgcgattc gtccgccacg atcaaaaaat
     9481 cttcaggaaa gaaatccaat agcgtataag gcggttcacc ttctttacgt ccgtccatat
     9541 gccgagaata attttcaatt cccgacgtgt agcccatttc taacatcatt tcaatatcgt
     9601 aattggttcg ctgttctaaa cgttgagctt ccagaagttt gttttcattt cgcaagacag
     9661 ttaaacgctg ttctaattcc tctttgatat tcgctacggc atgctccata tgttcatcat
     9721 tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc cgtttctcct aaaacttcac
     9781 ctgtcagtgc attgacttct cggatacgtt cgacctcatc tccaaaaaac tccacacgta
     9841 aggcacgttc atctcttgaa gccggaaaaa tctccacgac atcaccgcgc actcgaaaac
     9901 gaccgcgctg aaaatctata tcatttcttt cgaattggat actgactaag tcacgaatca
     9961 actgattgcg atctagttct gctccctgtc gaatcgaaac tacttgtttt tggtactcaa
    10021 aaggcgagcc taatccaaaa atacatgaaa cggatgcaat gacgatcaca tcatttcttt
    10081 ctaataatga actagtagcc gaatgtcgta atttgtcgat ctcatcattt acacttgaat
    10141 ctttttcga
//
LOCUS       DACEZN010000098        10064 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEZN010000098 DACEZN010000000
VERSION     DACEZN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.098"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(393..1241)
                     /locus_tag="IXH60_001929"
     CDS             complement(393..1241)
                     /locus_tag="IXH60_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1990839.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1405..2307
                     /gene="gnd"
                     /locus_tag="IXH60_001930"
     CDS             1405..2307
                     /gene="gnd"
                     /locus_tag="IXH60_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1990840.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2309..3310
                     /locus_tag="IXH60_001931"
     CDS             2309..3310
                     /locus_tag="IXH60_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1990841.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3307..3591
                     /locus_tag="IXH60_001932"
     CDS             3307..3591
                     /locus_tag="IXH60_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1990842.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3624..4421
                     /locus_tag="IXH60_001933"
     CDS             3624..4421
                     /locus_tag="IXH60_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1990843.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4435..5268
                     /locus_tag="IXH60_001934"
     CDS             4435..5268
                     /locus_tag="IXH60_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1990844.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5286..6143
                     /locus_tag="IXH60_001935"
     CDS             5286..6143
                     /locus_tag="IXH60_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1990845.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6165..6674
                     /locus_tag="IXH60_001936"
     CDS             6165..6674
                     /locus_tag="IXH60_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1990846.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6711..7112
                     /locus_tag="IXH60_001937"
     CDS             6711..7112
                     /locus_tag="IXH60_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990847.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7224..7577
                     /locus_tag="IXH60_001938"
     CDS             7224..7577
                     /locus_tag="IXH60_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990848.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7611..8054
                     /locus_tag="IXH60_001939"
     CDS             7611..8054
                     /locus_tag="IXH60_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1990849.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8071..9156
                     /gene="uxuA"
                     /locus_tag="IXH60_001940"
     CDS             8071..9156
                     /gene="uxuA"
                     /locus_tag="IXH60_001940"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1990850.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9149..9862
                     /locus_tag="IXH60_001941"
     CDS             9149..9862
                     /locus_tag="IXH60_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1990851.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3371 a         1588 c         2198 g         2907 t
ORIGIN      
        1 atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa taaaattaca
       61 aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt aattcaccaa
      121 aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag aaaagataaa
      181 ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg aataatacca
      241 atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta ttgatgttct
      301 gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg ctttttttgt
      361 tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga aatagtatgt
      421 cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa aatcacatat
      481 agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga tcggaattct
      541 gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga agaattagct
      601 tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc aatatggatt
      661 gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc cgtcaataaa
      721 gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat aggggaacgc
      781 aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc gaaaaaatag
      841 acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg ttgtaacaac
      901 ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc catagtgagc
      961 tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc attttcttgg
     1021 atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt tgtaaattga
     1081 aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg actatgactt
     1141 actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg gctgaagtcg
     1201 ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc tcattttcat
     1261 aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag agtaggaaaa
     1321 aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag
     1381 ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga
     1441 ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca
     1501 agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa
     1561 tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata
     1621 accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa
     1681 ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa
     1741 atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc
     1801 ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta
     1861 gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg
     1921 atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta
     1981 caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg
     2041 gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta
     2101 agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg
     2161 ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca
     2221 caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga
     2281 catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc
     2341 cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt
     2401 tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg
     2461 attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa
     2521 agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc
     2581 agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt
     2641 agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc
     2701 aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa
     2761 caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg
     2821 taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga
     2881 aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg
     2941 tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca
     3001 gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc
     3061 gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga
     3121 agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca
     3181 tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt
     3241 cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat
     3301 aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat
     3361 ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa
     3421 agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt
     3481 ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct
     3541 gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg
     3601 gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg
     3661 gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata
     3721 aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta
     3781 taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag
     3841 atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg
     3901 aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc
     3961 attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc
     4021 tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa
     4081 gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc
     4141 gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc
     4201 gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc
     4261 tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg gctgaattta
     4321 ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac
     4381 agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa
     4441 acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc
     4501 tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc
     4561 gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg
     4621 atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca
     4681 gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt
     4741 gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact
     4801 tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga
     4861 tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt
     4921 gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc
     4981 gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact
     5041 gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt
     5101 gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc
     5161 gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac
     5221 aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat
     5281 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
     5341 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
     5401 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
     5461 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
     5521 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
     5581 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
     5641 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
     5701 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
     5761 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
     5821 tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg
     5881 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
     5941 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
     6001 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
     6061 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
     6121 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
     6181 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
     6241 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     6301 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     6361 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     6421 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     6481 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     6541 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     6601 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     6661 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     6721 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     6781 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     6841 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     6901 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     6961 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     7021 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     7081 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     7141 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     7201 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     7261 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     7321 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     7381 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     7441 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     7501 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     7561 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     7621 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     7681 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     7741 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     7801 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     7861 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     7921 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     7981 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     8041 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     8101 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     8161 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     8221 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     8281 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     8341 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     8401 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     8461 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     8521 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     8581 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     8641 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     8701 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     8761 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     8821 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     8881 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     8941 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     9001 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     9061 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     9121 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     9181 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     9241 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     9301 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     9361 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     9421 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     9481 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     9541 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     9601 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     9661 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     9721 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     9781 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     9841 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     9901 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     9961 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
    10021 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tcca
//
LOCUS       DACEZN010000099        10050 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEZN010000099 DACEZN010000000
VERSION     DACEZN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.099"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            146..1333
                     /locus_tag="IXH60_001942"
     CDS             146..1333
                     /locus_tag="IXH60_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ1990852.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1314..2618
                     /locus_tag="IXH60_001943"
     CDS             1314..2618
                     /locus_tag="IXH60_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1990853.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2644..3657
                     /gene="citC"
                     /locus_tag="IXH60_001944"
     CDS             2644..3657
                     /gene="citC"
                     /locus_tag="IXH60_001944"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ1990854.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3661..3954
                     /gene="citD"
                     /locus_tag="IXH60_001945"
     CDS             3661..3954
                     /gene="citD"
                     /locus_tag="IXH60_001945"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ1990855.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3959..4864
                     /gene="citE"
                     /locus_tag="IXH60_001946"
     CDS             3959..4864
                     /gene="citE"
                     /locus_tag="IXH60_001946"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ1990856.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4857..6416
                     /gene="citF"
                     /locus_tag="IXH60_001947"
     CDS             4857..6416
                     /gene="citF"
                     /locus_tag="IXH60_001947"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ1990857.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEVVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6413..6970
                     /gene="citX"
                     /locus_tag="IXH60_001948"
     CDS             6413..6970
                     /gene="citX"
                     /locus_tag="IXH60_001948"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ1990858.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6963..7826
                     /gene="citG"
                     /locus_tag="IXH60_001949"
     CDS             6963..7826
                     /gene="citG"
                     /locus_tag="IXH60_001949"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ1990859.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            8060..8469
                     /gene="tnpA"
                     /locus_tag="IXH60_001950"
                     /pseudo
     CDS             8060..8469
                     /gene="tnpA"
                     /locus_tag="IXH60_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9106..10050)
                     /locus_tag="IXH60_001951"
                     /pseudo
     CDS             complement(9106..10050)
                     /locus_tag="IXH60_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         3447 a         1571 c         2017 g         3015 t
ORIGIN      
        1 ataccaaaaa aattgaattt agtctttttt taaattttta aaaataagca tcattttttt
       61 aaaatttaaa atgaaagggt ttttttattt taaaaaaagt gttaaagtgg ttttgtaatt
      121 aaaaaatgta aaaaggagtg aaggtatgct tgatgaagtt ttagaaatcc ataaaaaaaa
      181 tgtaggtgtt ttacggatgg aaacaacaga agccgtgcaa gatgatcaag atttagcaaa
      241 aatgtacacc ccaggtgtag cagaactaag tttaatgatt gagaaaaatc atgctttggc
      301 aagagaatta acaattagtg gtaaattagt ggccgttatt acagacgggt cggcggtatt
      361 aggtcttgga aatgtaggaa accaagctgg attaccaatc gtcgaaggga aagcgctgct
      421 ttataaaaat ttagcaggtg tcaatgcgat tccattagcc atcgaacaaa aatcagtaga
      481 cgagattgta caaacaattg tcaatttgca aaatagtttt gcagggatcc atttagaaga
      541 tattgcagca ccgaagtgtt ttgagattga agaaaaatta caagaaaaac tttctattcc
      601 tgtgtatcat gacgatcaag aaggaacagc gatagttgtt ttagctggat tgatcaatgc
      661 tgctaagatc caaaggaaaa cattaactga acttcgagtt ttgatcaacg gcatgggagc
      721 atctggggtt gcaacagcta gactattaat cgctgctggt atcaaaaatc taacattggt
      781 ggataaacaa gggagattaa agaagcaaga tagtacatta aataaatatc aaagagagtt
      841 gttggatcat atacatgata caaataataa tgcaagcgtt ttggaagatg caatcgttgg
      901 acaagatgtt tttatcggtc tttcaatagg aaatgtttta acgcctgaaa tgattggaaa
      961 aatgaatgat aatccgatta tttttgcttt agccaatcca gtaccagaga tcacacccga
     1021 tttagcaaaa gaatctggtg caaagctaat cgctacaggt tcttcgaaat atcctaacca
     1081 agtaaacaat attttagtat ttcccggatt gttcaaggga ctattactat cgaaagcgaa
     1141 taaagtcgat ctttcattac aacttgaagt agccaaacaa ttagctgcta aggtcgcaga
     1201 gcctacggta gaaaaattca tccctaatgt ttttgatgaa ggagtcgcaa acgtcgtttc
     1261 acaggcagtt ttggattatt tacaaaaact aaaagaaaac gaggataata tacatgctga
     1321 aaacattaaa tagaattaaa attggtttta ttcctttatc tctttatatc attttggtac
     1381 tcgcgtatct tgcaatgacg ataagcaata tcgttccaga aaatgatatg ttagtttctt
     1441 tgggtattat gactgttttt gcttatttat tagaagaaat tggtaaaact attcctattt
     1501 taaaagattt aggtggtaaa gtattagttg ttacattttt gcctgcattt ctagtctata
     1561 aacattggct acctcaaagt actgtaactg tggtttctga ttttatgaat aataacaatt
     1621 tcttgatgtt ctttatcaca ctactagttg tcggcagtat tgcttcaatg aatcggcaaa
     1681 cattggttaa agcaacttca cgtattatca ttattttaat tttatgcgat gtgattggtt
     1741 ctttgatagg aacaggtgtg ggaatactcc ttgggctatc tgcttggaat gcgtatttct
     1801 ttatcgttgc gccgattatg gctggtggtg ttggggaagg agcgatgcca ttatcgattg
     1861 gttatgcagc aatattatct ggtatgggac aaaatgatat ctttgggaag attctaccgt
     1921 gtgtgatgtt gggaagttta gtcgcagttc tactgtctgg tatgctcaaa aaaattggag
     1981 agaaataccc acaattaaat ggtcagggta atttgatcga tggggatgat gaacatttag
     2041 agaacttgaa gggttcagct aaaccagtca atattgaaaa aacattgtta gcaggcgtgg
     2101 tagcaataac gatttatttt gttgctgtct atgttgagca cttattaaaa tcactccttg
     2161 gcttttctat tccttcacct gtgatcttat tagtctctct tatgggggct aaaatgcttg
     2221 gttttatccc taaaaatatc gaagaaggtg gaaattcgtt gtatggattc actgttcgtg
     2281 ggatcacacc tcccttactt tttggcgtag gggttgcaaa aacagattgg ttagagctga
     2341 tcgaagtatt taaaaatcct gcaacgatag taacaatcat tgtgacggta gtagctattg
     2401 tcgctacagc ttttatcgcc gcaaaatttt taaaaatgta cccagttgat actgccatcg
     2461 cagtttcatg ttgtagcgga caaggcggta caggtgcttt agctatctta gctgcgggtg
     2521 atcgcatgga attaatgcca tttgcacaag ttgctgttcg actagggggt gctatgaccg
     2581 taacgttcgc tatcttttta atgggtctgc tatcataaga acataaagaa aggacgtgac
     2641 catgtgtttg atcaaacaga tatccaacga ttatttttaa aaaataacta tatcaaagac
     2701 caatgggaaa atcttttgct tgattcagga atacaaaaga agcaaatcga agattttcaa
     2761 catttagatc aaacattggg tatataccat aaagaaaagt tagtaggaac agtatcttat
     2821 caaaacaacg tgattaaata tatcgccgtg tctgaaaaat ataaagatgg tggacataca
     2881 tttaatgtac tagtctctgc aataattcag gcaatgagtc aacaagagat ttttcattat
     2941 attgttttca cgaaaccgcg ctatcaaaaa agttttaact ttttaggttt tgaaacaatc
     3001 gtatctaccc caaaaatagc tgtattagaa atgggagata gttctataaa acagtaccta
     3061 gagaggattc ctaaagtgaa tggtgaaaaa aaagcagcaa ttgtcatgaa tgcgaaccca
     3121 tttacagaag ggcattatta tttagtaaaa caagctgcca ataacaacga ttgggtatat
     3181 gtatttgtac tcgaaaaaga acaaaatctt ttttcgacga aggaccgact caaattagtt
     3241 caacaaggat tacaaaaact gaaaaatgtc attgttctat caggtggcga gtatattatt
     3301 agccctgcaa cgtttcccac ctatttttta cgagaagatg atgaaatagc aaaggagcaa
     3361 atggtcgtag atgctacttt attcaaagaa agaattgcca cttatttaga tatctctgct
     3421 agatatgtgg gagaagaacc actttcccca atgactcgtt catataacga catactggaa
     3481 acgattttac cccctgaaat aaaagttcat gtccttgcaa ggaagaaaac ggaacaacac
     3541 caagttattt ctgcttctca agttcgtaaa gcgtatcttg ctgggcaatt ggaaaaaata
     3601 aaatatatgg ttccagaaac aacctatcaa tacttaaaaa ataaaaggga gcgttaagta
     3661 atggaaataa ataaatcagc gattgctgga acattagagt caagtgatgt ccaaatcatg
     3721 atttcaccga atacagaagg aatagaaatc gatttagaca gtcaagtgat tgagcaattt
     3781 ggaaaacaaa tcaaaaaagt tatctatgac acactagcag tttatcagat taaaaacgta
     3841 aaactcaagg tagtagataa aggggcatta gattgcgtta ttgttgcacg attaaaaaca
     3901 gcgatccatc gttccttagg tatccaacac caagaaacac cgtgggaggt ttaattagat
     3961 gatgaaagaa cgtttgagaa gaacaatgat gtttgttcca ggaaataatc cagcgatgat
     4021 caaagatgca ggtatttatg gtgctgactc gattatgttc gacctggaag atgcagtgtc
     4081 tatggcagaa aaagacagcg caagaatgct tgtggctgag gcattgaaaa cagtggatta
     4141 tggtgacaca gaattaattg ttcgaataaa tgacctgcac tcagaatttg gacgtgcaga
     4201 catttttgca atggttaaag ccggcgcgca tgtattacgt ttacctaaaa cagaaacctc
     4261 tgaagatata acagaagtag aaaaagtgat tatagaagca gaaaactatc ataatgttcc
     4321 gatagggcga acattgatga tggcagccat tgaaggtgct tctggtgtat tgaacgcacc
     4381 tgcgatcgcc aaagcatctg atcgattgat tggaatcgcc attggggcag aagactatgt
     4441 gacaaatatg aaaaccaaac gatatccaga tggaaacgaa ttgttttttg ctcgcagtat
     4501 gatcgttcat gctgcacgtg cagcaggcat ttccgcattt gacactgttt actctgatgt
     4561 ggatgatatg gaaggatttt taaacgaagt acgtttaatc catcaattag gttttgatgg
     4621 aaaatcttgt atcaatccac ggcaaattcc agtagtcaat actgtttatc agccgtcaat
     4681 caaagaaatc aaacgatcac tagacatcat ctcggcaatc gaagaagcaa aagaaaaagg
     4741 ttctggcgta atttctttaa atggaaaaat gatcgataaa ccaattgtag aacgtgcaca
     4801 acggacattg atgttagcaa aagcatcacg attgattgat gaggagggaa atatccatgc
     4861 ataataaaca aagacgtagc attccatcaa gtgttttaac aaagtataaa aaagaattcc
     4921 aaagtgtttc attatcgcca caaacgattc aacgttgtgc accaaaagta acgactgtaa
     4981 ctaatcagac aaataaattg atgcactcga tcaaagaagc tatagaaaaa tcgggcttaa
     5041 aagatggaat gacgatttca tttcatcatc atttgagaga aggtgatttt gtttttaacc
     5101 aagtgatggc cgtgatccgt gaaatgggca tcaaagaatt gaccttagca ccaagttctt
     5161 taacgaacac tatgaatgag gtggttattg aatgcatcaa ggacggtaca gtaactcata
     5221 tcacaagcag tgggatgcgc ggcagcttag gtgaatttat ttctcatgga ggaatggaca
     5281 atcctgtgat tttacgttct catggtaatc gtccacgtgc aattgaaagt ggtgaattga
     5341 aaatcgatgt tgcatttcta ggagtaccaa catctgatga ttatggaaat gctaatgcga
     5401 ctgttggaaa atccgttttt ggttcattag ggtatgcaaa agtagatgca caatatgcag
     5461 ataaagtcat tcttctaact gatcatttag ttccttaccc taatacgccc attagtattc
     5521 cgcaaacaca agtcgactat gtcgtggaag tagaagcaat tggtgatccg aataaaatcg
     5581 gtgctggtgc tacacggttt actaaaaacc caaaagaatt aaaaattgct gaaatggtca
     5641 aagatgtcat tgttcactca ccttatttca aagatggctt ttcttttcaa acaggaacgg
     5701 gtggtgcagc tttagcagta acgcgttact tagctagtga aatggaagaa aaacagatca
     5761 aagcttcttt tgcattaggt ggcatcacta aaccaacggt tgatttattg gaaaaaggtc
     5821 ttgttgaaaa agtgttagat gtccaagact ttgacaaagg agcagctgaa ggaatggcgc
     5881 ggcttgagaa ccaacaggag atcgatgctt ccttttatgc tgatccaagt aataaaggag
     5941 cggttgttaa ccaattagat gtcgctattt tatctgcatt agaaattgac acaaacttca
     6001 atgtaaatgt tatgactggt tcagatggtg tgctaagagg tgcgattggt ggacatcaag
     6061 atgcagcaac ggctaaactt actatcatct cagcgccgtt agtacgtggc cgaataccta
     6121 cagtagtaaa tgatgttact acattgatca caccagggaa aagtatcgat gtattagtca
     6181 cagaagtagg ggttgctatc aatccccaac gtaaagatct aatagctatt tttgaaagaa
     6241 ttccacaaat tcctgttttt acaatagaag aattacagca aaaagcagaa aaaatagtag
     6301 gcatcccgga acctttgcaa ttcacagatc ggatagttgc ctatgtagaa taccgagatg
     6361 gttcaatttt agatgtcgtt cgtcaggtga aagaatttga agaagagaga tcatgatgtt
     6421 tgatactgga gaaattcaag aaatcgagga tattttgatc cataaagaac aacgtgctaa
     6481 cttgcaaagc aacctgcttt ccaagtattc aaatagttca ttgatggtct ttacattgaa
     6541 tattcctggg ccgatcaaaa ataatccaat gattgagcag gtatttcgtc agggacagca
     6601 gtcaattcag caaatgtttt taaaaaaaaa ggtggaaatg acgtacacaa aagaaatcaa
     6661 tttgccaagt ggattggact ggtacgtaat agtaacagaa gcacctttga atctcaaaaa
     6721 atggttggtc acgttagaag agcaagacgc attaggtaga ctttttgatt tagatgtctt
     6781 gttcaaaaaa ggagagcaga taacaattgt cagtcgccaa gctcttaatc atggggaacg
     6841 tccttgtttt atttgtggga aatcagcaaa ggtatgtgca cgtaatcgta cacatactgt
     6901 gaaagaaatg caaaagaaaa tcgaacaaat agtagccgat tccttaaaag gagcattgaa
     6961 acatgcatga taatattcaa aaaatagcac aagaagcaca attagcgatg ctttatgaag
     7021 ttacatgtat caataaacca ggattggttg atccagtaga ccccgggtct catcaagata
     7081 tggacatttt tacttttcta caaagtagtg tagttttgac tccctatttt gaagaattta
     7141 tccaaacagg attggaccga caacatcaac caattgaaga aacctttatg gcgattcgac
     7201 agattggttt agaggctgaa actgcgatgt tctcagtgac aaatggtata aatacacata
     7261 aaggtgctat cttttcattg gggatttttc ttgctatttg tggccggtta actatttgga
     7321 aagttccttg taaaaaaagt ttctttcaaa aagaaattca aaaaatgacg aaaaatctac
     7381 ttaacgattt tgggaagatt gatcaaacaa aaccaaaagc atggacttgg ggagaatatc
     7441 tatttgttaa ccatggattg acagggatac gaggggaagc caaaagaggt tatccttgtg
     7501 tttttgaccg aggattgcct tattatcaag catatcaagg gtcgcaacaa gataagatga
     7561 tagacacttt actttttcta tcaatggaag tagaagatac caatttgatc aaacgaagta
     7621 ataatagaca tgtttttgaa gagtatcaat tacttattcg tccttatttt gaattagggg
     7681 gtgtaaaaac catacaaggt aaaaaatatt tggattacct aaatcagcaa tttaaaaaga
     7741 aaaattggag tattggtgga agtgcagact tattgatcct tacaatcttt ttagataaaa
     7801 tcatggacaa aggatggtta tgttgatact ataatacatt attttgtatt agtaagctaa
     7861 taactaaaaa atggttcaat acaaatattt tataaaaaat ggtagtgcct atcctttcag
     7921 attgtagaca aagaaaaatt tctttgtcta caattttttt gttaatgact tcagtcagtt
     7981 tcgtgcgcaa agcgtgcgaa acaagaaaac catgcgtttc tacacgtgga agaactagca
     8041 tatcgaaagg agttttttca tgtcaaatga cgataaaagt ttagcccata ctcgttggaa
     8101 ttttaagtat catattgtat ttacaccaaa atatcgacga aaagtaatct ataggcaatt
     8161 acgaaaagat attggaaaaa tacttaggaa gttgtgcaaa ataaaaaacg tggaaattat
     8221 agaagcacat gcaacgccag atcatattca catgcttgtt caagtaccat caaaaataag
     8281 tatatccagt tttatagtta tctgaaagat cgtagtgctg tgctaatcca cgagcagcac
     8341 gtaaatcaca aatatagata cggaaataaa tcattttggt cggaggaata ttatgtgagt
     8401 acagtcgaat tgaatcaaaa gacaattgaa aaatacatcc gtgaacaaga agctgatgat
     8461 cgcatttgag atagtataag taaaagagag tagatgttgc aataacaaaa gggtaaaaat
     8521 ggactacctc cagtaaaata ccgaaaaata gtttaacttt tggagataag tccatttaag
     8581 ggtctataga atgacaattt ttttagggga ttttactatt attacttcaa gacccgcttg
     8641 gattcgcaaa gtgagtgaac tcttaaaact acctgatagt aggttgtttt aagagttatc
     8701 tgaaagtaaa aattgcattt atatttttga taaatacgta aatttatata tacgtattta
     8761 tcaaagtttt aggccaaagg aatattgtgt aaatacacta ggattgaatg aatagaccag
     8821 aagaaattgc gtccaagaac aaaagaagca tgatagtgct ctatataaat tgggcattca
     8881 agaatctgaa atttaaaggt agaattttag ttagactttt agattttaaa cacaaaaata
     8941 accatacaag caacaagaag caaagtgcta tattataaat tgtccgtccg tttacttaca
     9001 taagatcatt ggggctattt ttaaaagaga ataatagata ataaatgaaa aaaaagtcaa
     9061 gtccacaatc ctgtgtaaaa tactatacaa tgctctcact attttttact tggcaaattt
     9121 aatatatttt cttgatgaag aaagccactc atcgtgttga tcaataagga ctgctccaat
     9181 taaacgattg gctgaaacgc agtttggaaa gatacgaata attttttctc ttctgcgcac
     9241 ttcttgattc agtcgttcaa ttagattggt actctttagt cgattgtggg aatttccttg
     9301 tacggtatat tgaaaggcgt cttcgaatcc atcatccaat gatgcgcaag cttttgaata
     9361 ttttggttga tcgatataat catgaatcaa tcgattttta gcctcacgag ctaagttaat
     9421 atctgtgaac ttaaaaattc ctttaacagc ttctctgaaa gattttgaat tttttttagg
     9481 aatggtggta aagatatttc ttaggaagtg aacttggatc tttgccaact tgcgttggtg
     9541 aaggattttc taatggcaga gactaatcct ttgtgcgcat cagaaataac gagttctgta
     9601 ccttgtaaac cgcgttcttt taggtattca aaaaatgtta tccaggtctc ttcgctttcg
     9661 ccactttgaa tcatgaagcc gataatttca cggtcgccat ctttggttat tccaatcgct
     9721 atatgacagc tttttgagag tactcgattt tcttctcgta cttttatata gagtacatcg
     9781 gtcattaagt aaggataatt tttttctgat aataaacgat tctgccactc gttaaccatt
     9841 ggttctagct gttctgttaa gctagaaacg aaggacttag agacggattt accacaaagt
     9901 tcttccacaa tttttgatac tttacgagtt gaaacgcctg atacatacat ttccaacatt
     9961 gaagcaatga gggctttttc gtttcgttga taacgttcaa acactgtggg tgaaaaatgg
    10021 ccatcacgtg ttctgggtac ttttaattct
//
LOCUS       DACEZN010000100        10049 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEZN010000100 DACEZN010000000
VERSION     DACEZN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.100"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IXH60_001952"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IXH60_001952"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1990860.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IXH60_001953"
     CDS             complement(1172..1867)
                     /locus_tag="IXH60_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1990861.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IXH60_001954"
     CDS             complement(1880..2596)
                     /locus_tag="IXH60_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1990862.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="IXH60_001955"
     CDS             2926..3531
                     /locus_tag="IXH60_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1990863.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="IXH60_001956"
     CDS             3856..4179
                     /locus_tag="IXH60_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1990864.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="IXH60_001957"
     CDS             4361..5731
                     /locus_tag="IXH60_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1990865.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="IXH60_001958"
     CDS             complement(5861..7162)
                     /locus_tag="IXH60_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1990866.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="IXH60_001959"
     CDS             complement(7392..9383)
                     /locus_tag="IXH60_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1990867.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="IXH60_001960"
     CDS             complement(9527..9793)
                     /locus_tag="IXH60_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1990868.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2943 a         2083 c         1829 g         3194 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacag
//
LOCUS       DACEZN010000101        10039 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEZN010000101 DACEZN010000000
VERSION     DACEZN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.101"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            24..1763
                     /gene="yfcC"
                     /locus_tag="IXH60_001961"
     CDS             24..1763
                     /gene="yfcC"
                     /locus_tag="IXH60_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1990869.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1920..3104
                     /gene="iadA"
                     /locus_tag="IXH60_001962"
     CDS             1920..3104
                     /gene="iadA"
                     /locus_tag="IXH60_001962"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1990870.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3215..3418
                     /locus_tag="IXH60_001963"
     CDS             3215..3418
                     /locus_tag="IXH60_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1990871.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3519..4181)
                     /locus_tag="IXH60_001964"
     CDS             complement(3519..4181)
                     /locus_tag="IXH60_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990872.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4194..5696)
                     /locus_tag="IXH60_001965"
     CDS             complement(4194..5696)
                     /locus_tag="IXH60_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1990873.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6244..7923
                     /locus_tag="IXH60_001966"
     CDS             6244..7923
                     /locus_tag="IXH60_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1990874.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8050..8529
                     /locus_tag="IXH60_001967"
     CDS             8050..8529
                     /locus_tag="IXH60_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1990875.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8748..9428
                     /locus_tag="IXH60_001968"
     CDS             8748..9428
                     /locus_tag="IXH60_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1990876.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9436..>10039
                     /locus_tag="IXH60_001969"
     CDS             9436..>10039
                     /locus_tag="IXH60_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1990877.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKT"
BASE COUNT         3173 a         1775 c         2190 g         2901 t
ORIGIN      
        1 aaaaaagaaa ggaagaaata aatttgaaga aaacaaattc ttcaggaaaa ccgaaacgcg
       61 acttcactaa attatcgaca ccccatacgt atgtcattat ttttggtgta gtcatctttg
      121 catggatact gacttttgtc gttccagcag gaaagttcag cacgcaagat attgaataca
      181 aagatgcaaa tggcgaaaca agtactcgta ctgttttacg tcaagattcc ttccgttatg
      241 cttacgagct ggataagtcg tatgtgtttg atcaattaga agaactgcaa gatcatccag
      301 cagaacgtga aaaactggat gttccggaaa aaggattgga aaaagtcatt gctgacggag
      361 aaaaaaatct tacgcaggaa aaactagatg aaatctcctt gacagatgat gtgctttacg
      421 atcagtacgg cgaaaatatc tatgatactt ctaaaaaatt gcataaaaca gccaaaatat
      481 ggggaacaga tgattttggc ggctttggtt tcttaaattt tgtttttgaa ggactagtat
      541 ctggagataa gtatggatct gctgtaggga tcgctgcatt gatccttgtt gttggaggtg
      601 cttttggtat tattatgcgt accggagcga tcgacgcagg tatctatgcg tttatcagta
      661 aaactaaggg actggagcgt ttagctttac cgctattatt ctttgctttt tcattcggtg
      721 gcgctacatt cgggatggca gaagaggtaa ttccattctc aatggtgatg gttccatttg
      781 tcattgcgtt aggatatgac tcgatcgtag cagtaaccgt gacttatgtc gcatctcaag
      841 tcggaaatgc gacctcttgg atgagcccgt ttagcgtagc ggtggctcaa gggattgctg
      901 gaatccccgt gttgtctggt gcgacattcc gattgattat gtgggtagtt gttactgcat
      961 tagccgctgg ttatatgatg atatatgcag aaaaaatccg taaaaaacca gaaagttcgt
     1021 tgacttacaa atcagatgat tatttccgtt ctcatatcaa aaagacatct gatgaaaata
     1081 aaaaattcat gcttgggcat aaattgatct tattagagat gttagttgtc cttgtctgga
     1141 tcgtttgggg cgtgactcaa aaaggctatt acattccaga aatcgcttct caattctttg
     1201 tgatgggatt agttgctgga atcattgctg ttcttttcaa attagatgga atgaaagtca
     1261 atgacatcgc ttcttctttc caatctggtg cagctgattt agctggtaca gcaatcgtag
     1321 taggtatggc caaagggatt cttcttgtct tgggaggatc tgatgcatct gttccttcag
     1381 ctttgaatac gattttgcat gggatcggga cggcgttgac cggtgttcca gctgtcattg
     1441 gtgcttgggc gatgtacatc ttccaaagtt tgttcaactt ggttgttaca tcaaattcag
     1501 gacaagcagc tttgactatg ccaatcatgg ctccactggc agatttagta ggtgtatcca
     1561 gacaagtagc agttcttgct tatcagcttg gttcaggttt tatggacgct tttacacccg
     1621 tatcagccag cttgattggt gtgctaggtg ttgcacggat cgaatgggca aaatgggcaa
     1681 gattccaaat caagatgcaa ggattcctgt ttgttttagg aactattttc atcatgattg
     1741 ccattgcaat aggtctgcag tagtactgga gacctgtaaa taaaaaagta atccaaacag
     1801 tagataagga ctccaagaac cgtcatttta cggtaaaatc aaaggagtaa agatcctatc
     1861 gtttggatta ttttttaaga tcctatcatc agttatactt aaagaaagag gaaaaatgta
     1921 tgaaaatcat tcgacaaatt gaagtgtttg caccagatta cttaggaaaa atggatgtac
     1981 tgattgccgg tgggaagatt ttggctgtag aagaacagct agaaggcggc tacgaaggag
     2041 tggaagtaga agaactttca ggtgagggaa aagtactaac cccgggtttc attgactgtc
     2101 atttccatat tttaggtgga ggaggagaag gcggttatca aaatcgaacg ccagaagtca
     2161 cattgagcca attgactaca gctggtgtaa cgacagttgt cggttgtttg ggaacagacg
     2221 gagaaggtag agatatgact gcgttgatca gcaaagcgaa aggtctggaa gcagaaggaa
     2281 tctccactta tgtatacgaa ggttcgtacc gattgccggt aaaaacagta acagattcta
     2341 tcatcaaaga ctttttgaca attgataaaa ttatcggaat cggagaaatc gcagtatccg
     2401 atcaccgttc ttctcagcca agttttgaag agttcgctcg tgcagtagca gatgctcgtg
     2461 taggaggcat gttatccgga aaagctggga tcatcaatgt ccacttaggc ggaggaaaaa
     2521 gaaagctaga gctattgaca cgaatcgtag aagaaacaga aattccgatc acacaatttt
     2581 tgcctactca tgcaaatcgc acaccagaat tatttgaagc ttgcgtggca tttgccaaac
     2641 gtggtggcac gattgacttc actgctagcg aagatcctga tttttgggag aagacagatg
     2701 gtgaagttcg cttcagcaaa gcactgaaac gattgatcga ggaagatgtt tctttagata
     2761 atttcacgat gacctcagat ggacaaggaa gcttgcctta ttttgatgaa aacaatcatt
     2821 tcctagggtt aggagtagga agtgccaagg cattgctagt agggatcaaa gaagctgtgc
     2881 aaaaagaaag tatccctttg gaaatcgcct tacgagctat aaccagtaat ccagcacgta
     2941 tattgaaatt ggataaaaaa ggaaaaatcg aaattggtgc tgatgcagat ctgtgtatct
     3001 tggataaaga aacgcttgat attgatacag ttatcgctaa aggagaaata atggtccaag
     3061 aaaaagaagt caaagtatgg gggactttcg aaaaaagttt ttaatcaaca gctctaacta
     3121 tcttttttag aaaacgagcg ttctgtttgt ctgtatctgt tgttgatgtt accctgaagg
     3181 tagatcaata aacaaactaa gaggaggaag cacaatggca gatttaaaag gacgttctaa
     3241 agatatgaaa gataaagtag tcggtgaagg aaaagaagca tacggcaaag caacagacga
     3301 caagagcaaa gaaacagaag ggaaagcaca atcactaggt gctgacatca aagaaaaagc
     3361 tcgaaattta ggcgacgaag tcaaagacaa gttttctcga aaagacgaaa aaaaataaaa
     3421 accacttatt actaattgaa tcaagcaaat aaaaaatccg aaccatatct gcgagacaga
     3481 tatggttcgg attttttatt tgatcattat cctgtttttt aaacttcgat tttttgaaat
     3541 tttttatttt ttagttcaaa gacttcatca cagctttttg ctacttcaat ctcatgagtc
     3601 acgatgataa tacagcgatt ttgttcatgg gcgatttctt ggaaaagttt gacgatatct
     3661 tgtgaagttg tttcatctag atttccagta ggctcatccg ctacgattag ttgatgttca
     3721 cagcagatag cacgaacgat agcgactcgc tgctgctgac ctcccgaaag tttggtcacc
     3781 tgtttttttg ctaatatttc atcaattcct acacgagcca gtcgatctaa agcaacttgt
     3841 cgataattct cttgcttggt cttagtgatt tccattgctg ctatgacgtt gtctagagcc
     3901 gataaataag gaagcaagtt gtaagcttgg aaaataatcg agacatcatt tttacgataa
     3961 cttcttaacc cgattttgtt cagtgaacga tcttgatagt atacctttcc ttcttttggt
     4021 gcatctagtc cagaaataag cgaaagaaaa gtcgtttttc cagaaccact tgctcctaaa
     4081 atagcgtaca tttttccagc ttcgaaattc agttctacat ctttataaag cgcatcttgc
     4141 ggatcttgat accaaaatcc caattgttct gtccgtaaca tcaaattcct cctttagttt
     4201 aataggattt ttcttgggtt taatcttaat atccctatag aagctaaaaa gacagaaaac
     4261 agactgattc caaatgctac tccggctaat agtcccaatt gtgcaggttt gacattgatt
     4321 tccaattcgc tgacttgatc agaaacagca aatggattat tcgtggttcc ttgttgacca
     4381 tcgcgttgac cgccttgttc ttgtctgtta cctggcattt gtccaccgcc aggagcttgc
     4441 tggttttgtg tcgtggtttc tgtctgttgg tcaagtagct gctgtcccac ggcattcgcg
     4501 acgacgtttc cgctaagcga agcaactcct aatgcaagaa tcatacaaat agccacttcg
     4561 gtaaacaatt ggagaatgat tttacttctg gattctccta aagatagcaa gacgcctaac
     4621 tcatgccgac gctcccgaat ggtaatcata atgatcagag taagtatgat aataccggct
     4681 acagccacta gtaaaacaac atttttagaa aaacttgcca cgttgtttaa aggttccaac
     4741 attgactgat acatggaatc atttgattgt aagctaaatg tatctgtgtc gatcagcttc
     4801 tcagcttctt tgagaaactc atccatttta tctggatcag atagagtgta aatagcagaa
     4861 tctatcgtgt tatcgtcaga tgtcccattt agttcatttg caaaagtgta agaagtgtaa
     4921 agtgtattcg acggattcat gaagttgaat ttcataccca tactgccgct tgtttcgctg
     4981 gattcataaa ttcctttgat cgtcatttca taagtggtat cttcatcttc agtactcgtg
     5041 attgtgaaag tatctccgac tgccaggtca tttgtttctg ctagagtaga atcaatgact
     5101 acattgtttg tgcctttgtc gctgtcatct attccttcac catccgtgat ttttgctgtt
     5161 ccatcagaaa aattactatt ttgtgcagtt gctgacacac cactgatttg aaagtctgct
     5221 tgagtcattt gcggattccc gcccattcct ccagcaggtt caccgcctgc atcagtagtt
     5281 tctgaactgt cttcacttga atcagaacta gtgatggcag ttattccatc ttttgccata
     5341 gctgaggcgg aactttcaaa ggaataactc tttacattat ctaattcagc tattttttcc
     5401 gcgtccgtta aggaaactgg tgtaagagaa aaactgcctg gatctggacg ttcttcagtg
     5461 gtagaaccgc ttgaagaagt ttgttcctgt tttttaaatg aagcttctcg attggcactt
     5521 aaagtcactg ttgctccaac ttctttttgt gcttgattcg ctgcttcttc tgcagcgctt
     5581 cgaatcgtga ggccagctag cacaaaaata agaatggctg tgaacacggc aaacaataac
     5641 aatgaacgtc cccattttac ttttgtactt ttccatgcac gtttcataaa attcatattt
     5701 tgcatccctc ctagtagatg catttgataa tagacaacct atcttaaagc tagcttaaat
     5761 cttatgctgt ttgcacacca tcaacatgag gttctgtata tataaaaagt cactatagtt
     5821 attgcaaaaa atgtggtaaa atgaaaaaag tatgtttcaa accgacgaag aagaaatgaa
     5881 agaggaatag caaaagaaat cctagatgct acagagggat ttttacgtgc atagttgttc
     5941 taacaattgc tgatactagc gatttagttt atttgttgta taataatggg gaacagctga
     6001 atacttatat aagtattctg gctatagcgt ttcgcaaaag atccattttg ttaatgaagt
     6061 atatccaaaa taaaatgccc atccaactgt cagaacgcgg atgtgctggt aattttcctg
     6121 ctgattctcc ataagttgag aaatagtgat tttgcatatg cttcattgga atggctcata
     6181 tatttagata aagaagaaaa tcgacatctt taaacttgga tgtcataaga agaggtgaag
     6241 tttgtgagta ccattaaaat tatcccatta ggcggcgttc gtgaaaatgg aaaaaacatg
     6301 tatgtcgcag aagtgggcga ggatattttt attttagatt gtgggctaaa atatcctgaa
     6361 aatgagttgt tggggatcga cgtcgtaatc cctgatttta cgtatttaga agaaaacatt
     6421 gatcgtgtag ctggtgtctt tttaacacat ggacatgcag atgcgatcgg ggcacttcct
     6481 tacttgttgt caaaaatttc tgtaccagta ttcgggacag agttgacgat cgagttagca
     6541 aaattgaatg tcagtcgtaa tgaagcagcc aaaggattta aggatttcca tgtggtagat
     6601 gagcatacag aaatagactt tggtgaaaca gtagtgagct ttttccgtac cactcatacg
     6661 ataccagatt cagtggggat caatctgaaa acaaaagaag gtagtatcgt ttatacggga
     6721 gattttaaat ttgaccaaac ggctatccca atgtatcaaa cagattatgc acgattggcg
     6781 gaaatcggca aagaaggagt cttagcctta ctaagtgaat cggcaaatgc agaaaatcct
     6841 cagcctgtag cttctgaagc ccaaatagca gatgaagtat ttgatacgat caaatattgg
     6901 gaagggcgta tcatcgtcgc ttgtgtagca agtaatttgc aacgagtgca acaagtattg
     6961 gatgcagcag aaaaagcaca acgaaaagtt gtcttgactg gtcaagattt cgaacgaatt
     7021 attcgcacag cgatgcgttt agaaaaactt caattaccaa gtgaagatct gcttgttacg
     7081 ttgaaagaca tgaaaaacta tgatccagaa gaactgatca ttttagaaac tggccgaatg
     7141 ggtgaaccca tcaagtctct gcaaaaaatg gcaaacggta cacaccgtaa tgtcaagatt
     7201 gaagaaggag atttggttta tatcactact acgccaagca tcgcgatgga aaccatcgtg
     7261 gcgaaaacgg aagatatcat ttatcgagct ggtggaacag tgaagttgat ctctgagaat
     7321 atgcgcgttt ctggccatgc caatcctaat gacttgcaat taatgatcaa tttgatgaaa
     7381 ccaacatatt ttgtgccagt tcaaggagag tatcgtgaat tagctgctca tgcagatttg
     7441 gctcatgcag tagggatgcc atacaaaaat atctatatta ctggacgcgg agacgtgctg
     7501 gaatataaga acaagcgtat gagtgttgca ggaactacaa gtgcagaaaa tgtaatgatt
     7561 gatggattag gcataggtga tatcggaaac attgttttac gtgatcgaaa aatcttgtca
     7621 gaagacggga ttttcgtagc ggttgtcact atcagtcggc gagaaaagaa aatcatttca
     7681 aaacctcaaa ttacttcacg gggattcgtt tacgtaaaag ctagccgtga tctgatgaaa
     7741 gaaagcagca atatgattga aacaatcgtt gagaaacatt tagaaagcaa tgattttgag
     7801 tggagcaaat taaagcaaga catccgagat caattaggcc gttacttatt tgaacaaaca
     7861 aaacgccgcc cagtgatctt gccagtcatt atggaagcta ctcaacgtcg aaataaaaaa
     7921 taaacaaaaa gagtaagatg aacctctcta tagagtgctc gtcttgctct ttttttgcga
     7981 aaaacaaata atttttgaat acagcatccc tcgctttata ataaacgata gaataaaggg
     8041 agtgaatcaa tgatgagtca aacaaaagtc gaacgttatt tagaagaaca aaagatcagc
     8101 tatcaaccac ttgattttag tcatgtggta gaagaatctt tcacagaaga actacaaaaa
     8161 agagggattg atccagcttt gatatgtaaa actctcgtag tgaaaggaaa caaaactggg
     8221 gtgatcatcg cattgattcc tttgaacgat cacttagatt acaaaaaaac gagacaagtg
     8281 acgaaagatc gtaaagttgg ttttcctgga atggattttg tattaatgca tacaggatat
     8341 ccccacggag caaacacccc tattggaata aaactcgcac atcctgatta ttgtttttta
     8401 gcagacagtt ctttaaaaga aaaagaagaa gtaattgtgt caagcggtga aatcgggaga
     8461 agccttcgga taaaagtagc cgatttagaa aagatcgtcc atcctattta cgctgattta
     8521 attagttaag ggacgagaag agaagctaag cagagcagag acctcaaact tgagattata
     8581 tctgcttggt acttggtgca tcctgtatta tctgttacaa ttgctttgta catactgtac
     8641 tatcaatcca atgaaaatat atttagcagc tatatttttt cattgatgtg caagtgatga
     8701 aatcgttttt aataaaaaag atacagagaa aacgcgaggt gtaaaatatg aaaattacgt
     8761 atcatggaca ttcctgttta acaattgaaa tggaaaatgg ccaacgcgtg atgattgacc
     8821 cgtttattac aggaaatcca ttgacagact taactgcaga agaagtaaag acagattggt
     8881 tgctgattac tcatggtcat agtgaccaca taggagatat gattcctatt gcaaaagaaa
     8941 atcaagccac aattatcagt attgttgaga ttgctgatta tgcgaacagc agaggagtag
     9001 attcattcgg tatgaatatc ggtggaaaac atgcgtttcc attcggcgtg gtaaaattcg
     9061 tacatgcaca gcatagttca agctatgaag tagacggtat cgttcagtat atgggcgagc
     9121 ctagcggaat tattattcaa gcagaaggaa aaacgattta tcatgcaggg gatactgctt
     9181 atttcagtga tctaggattg cttgcagaag aattcgatat tgatgtcgct ttcttgccga
     9241 ttggcgataa ctatacgatg gggccagaag atgcagcaaa ggctgcgcaa acaattcgtg
     9301 ccaagaaagt agttccaatc cattacaata ctttccctgt cattgaacaa gatcccgaat
     9361 cttttgtaga gttactggaa gatagtgaag gaaaaataat gagcgtctcg gaaacgctgg
     9421 aagtgtaggc agaaaatggc gacaaaacat gatttgattt tagaatatat tgaaagttta
     9481 cctgttggca atcgaatttc tgtaagaagc atcgcaaaag aattgaatgt cagtgaagga
     9541 acagcgtacc gtgcaatcaa agatgcagaa aatgtcggac tagtttcaac aattcaacga
     9601 gtagggacca tccgtatcga acgcaaatta aaaaaacata ttgaacgttt gacatttggc
     9661 gaggtcgtca ggatcattga aggggacgtt ttaggcgggg cggcaggatt agacaaggtc
     9721 ttgaataaat tcgtcatcgg cgcgatgact aaacatgcca tggaacgcta tattacgcca
     9781 ggatcactaa tgatcgtcgg caaccgggcg gaagtccaaa aattagcatt ggaagatggc
     9841 gccgcagtat tgatcacagg aggattcaat acgactgctg agattgcaaa gctagctgat
     9901 gatcttgaaa tgccgatctt acgcacctct tatgatacat ttacagtcgc aaccatgatc
     9961 aaccgagcgt taagcgacca attgatcaaa aaagatatca tgcttgtcag tgatatctat
    10021 atgccagctg aaaagactc
//
LOCUS       DACEZN010000102         9700 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEZN010000102 DACEZN010000000
VERSION     DACEZN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.102"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1023
                     /locus_tag="IXH60_001970"
     CDS             <1..1023
                     /locus_tag="IXH60_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1990878.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="IXH60_001971"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="IXH60_001971"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1990879.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3389..3985
                     /locus_tag="IXH60_001972"
     CDS             3389..3985
                     /locus_tag="IXH60_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1990880.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="IXH60_001973"
     CDS             3998..4897
                     /locus_tag="IXH60_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1990881.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="IXH60_001974"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="IXH60_001974"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1990882.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="IXH60_001975"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="IXH60_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1990883.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="IXH60_001976"
     CDS             5430..5663
                     /locus_tag="IXH60_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990884.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="IXH60_001977"
     CDS             complement(5822..5944)
                     /locus_tag="IXH60_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1990885.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6134..6358
                     /locus_tag="IXH60_001978"
     CDS             6134..6358
                     /locus_tag="IXH60_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990886.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            6801..7007
                     /locus_tag="IXH60_001979"
     CDS             6801..7007
                     /locus_tag="IXH60_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1990887.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7007..7258
                     /locus_tag="IXH60_001980"
     CDS             7007..7258
                     /locus_tag="IXH60_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990888.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            7439..7711
                     /locus_tag="IXH60_001981"
     CDS             7439..7711
                     /locus_tag="IXH60_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990889.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            8156..8335
                     /locus_tag="IXH60_001982"
     CDS             8156..8335
                     /locus_tag="IXH60_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990890.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            8394..8750
                     /locus_tag="IXH60_001983"
     CDS             8394..8750
                     /locus_tag="IXH60_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990891.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         3485 a         1613 c         1995 g         2607 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     2341 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatcct attatagctt atgatccaga aaaaaccttg tgtgacattt ggaatccctg
     3841 gtataacgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactacatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaattttg cctacagata aaacgatgcg
     3961 ctcttatatt atggctttaa attaaaggag aatacacatg acacctacgc aattaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttaattg gctcaaagta tggcattgaa aatcgaacaa ctaaagacat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttgacaacgg ttctaaacga
     4261 tattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagggaagct gattattatc cagggtttag cttacgtgta ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgttactac aggtgactcg atctatcctg cgacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttgtcaga ccggcaacaa gttgcctttt atcaccaaaa
     6661 agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtcttgtcgc ttttcgttga
     6721 ttcattctgg ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct
     6781 aagaggaggc agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg
     6841 aattattccc aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga
     6901 aatgtatcaa aagctattgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt
     6961 cttatggcga ttatatttta attgatgaga aaggaaagaa acgctgatga aaacacaaca
     7021 caaattattg actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga
     7081 aaaagtagca gaagttttac ttattccgcg gtactggacg atagaaaccc ctttagtaga
     7141 ggatgcaccg actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta
     7201 tcaagaaaac ttggaaactt atgaaaacga atattggtta accaaattag agaactaaaa
     7261 aggagaaaaa aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa
     7321 aatggcacaa aaaatggagg atatgacgtt cctatacaac gaaacacgtg ttccaaaaaa
     7381 acattataaa gaaaaattgt cggtagctgt ggaagaaatg attgaataag gtgtcgaaat
     7441 gaatcttatt gcgacgtact atcgaacact ggaagaactg aaaaagcaaa atgcaaaatg
     7501 gtttttccaa gccttactat gcttggaagt aggtgtcaaa ccaagcacta ttaaaccttc
     7561 tgaataccaa gctttagaac taacgtatgc gaaatttatc gaaacgaaaa aagccaagac
     7621 tgtttcaagt gaatggttag attattttga aaatatcaat aagtatggcg catattacac
     7681 catgaaaaag gaagacaacg aaaatgaata aggtgattta tcaactaaca acggattcca
     7741 tatcaacgat ttgaaagaaa aaaaggtcaa taatcattta taccgaaaag tgagaatgtg
     7801 tgaaacaagg acaaaaaacg cctagtaact aaggaaatgg ttcttatttc gcaccaatcg
     7861 ctccaaccgc ccccgccgct taggtggtgc gaaataaacg ggcaaagccc gataacttaa
     7921 gagaaagaag gtgatccatt tgaacaagat tattccctta ttgttagtac tgtttgttgg
     7981 cgaaatactt atgcgagata gtgcagctgt acagttaaac ctcgctgtat ttttggtaat
     8041 actggtgttt tgtttgatct tttggcggtt tgtattagtc ctcgtgagtc tactattcaa
     8101 gatgattgca gccatatttt tcatagggtt atttcgatga aaggagtgag taccaatgcc
     8161 aattgcaata ggaaataaac ggttacccgt gacattagat gaaaagagac aaaaagaatt
     8221 gcagcaacta aagcagaagt atggcaaaag tgaatctaag attatgtgta ttgcgttaga
     8281 tctattgatc gcgcaagaaa aagcaggatt tgatgtacca gcgctaaaaa agtgacgtca
     8341 ctttttaatt tgatctatat aagtgatatc acttttacca aggagggaat taaatgttaa
     8401 aaactaaaaa gaattcgtac aaattaaagc ttagtatact atctattatt tgtcttttag
     8461 gcatgataat tagtggtcat ttgtatagag tggcatatac gcagtttatt gagtacaagg
     8521 ttcactcttt aggattagct ggggatcgat acgttgaata tgtccattat tcattaaaat
     8581 gttcttcata tattcttctt tggcttattt tttcattgtt tgaattgcta aatttaatag
     8641 caatatcttc atatcactta gataagctaa ataccaaatc tagccgttat aacgaagtgt
     8701 ttaaggaaaa tgtgaagtta gaaagtgact taaacaagct aaaaaaataa aaagaagaaa
     8761 acataagtta actaaaatta ttttattaag aatgatggaa tataaggata aggttaccaa
     8821 cataacgatc attacttctc tgatatgcta tggacttcta tcaatcagta ttttgtctat
     8881 agattatgga gtaactatac tgcaagcctt agttttggct atgcctatgt atctatcttt
     8941 gtttatcatg catgaaatat ataagctatt tgttttatca aaaaatgtag aaatacgggt
     9001 aaatattttg gtgaatagtt atcaaaaaga actcaaagaa agaagacggt ggcggtattt
     9061 attgaaagga tgaaaaagta ggatttgaca tactggtatt cagaaagtgc cgtcactttt
     9121 aaaacaatgg aaaaggtgga gataagatga agaaaaaaca aactaaacta tcattagcag
     9181 atatttaaca cagttaaccg caactgaaga tggtgttgtt gaatttgaac aatctgaaat
     9241 tgcggtagta gatgatcgtt accttaaaat gccttacttc tttgaccaag caaaggtcat
     9301 ttgcctttgt cactacgatg gggtacgtga ttttaggcgt taaaaagtag tgattatatc
     9361 aactttacga gtaaatataa atttagacat gaaactgaat tatccaatgg aaaatcatgg
     9421 tttataatct agaaaaatta tagtaatgat tggattatat atattctttc gtattttcgt
     9481 acaactgtag cttgtcaaaa ttgagaaatt taaccgatta ggtactttac atcgttattt
     9541 gggtaatttc taaaacatga agcttaccaa cgaataaact gagactatct tattaattga
     9601 tcaatataag aaaatatctt tgaggagatg atgtactatg ttttcgtcta tccacgattg
     9661 gttaggtggt cttttcggcg gtggaggtaa aggtttttaa
//
LOCUS       DACEZN010000103         9689 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEZN010000103 DACEZN010000000
VERSION     DACEZN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.103"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            282..521
                     /locus_tag="IXH60_001984"
     CDS             282..521
                     /locus_tag="IXH60_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990892.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IXH60_001985"
     CDS             complement(883..1143)
                     /locus_tag="IXH60_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990893.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IXH60_001986"
     CDS             complement(1328..1825)
                     /locus_tag="IXH60_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1990894.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IXH60_001987"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IXH60_001987"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1990895.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IXH60_001988"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IXH60_001988"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1990896.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="IXH60_001989"
     CDS             complement(4547..5209)
                     /locus_tag="IXH60_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1990897.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="IXH60_001990"
     CDS             complement(5219..6034)
                     /locus_tag="IXH60_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1990898.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="IXH60_001991"
     CDS             complement(6296..6739)
                     /locus_tag="IXH60_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1990899.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="IXH60_001992"
     CDS             complement(6873..7211)
                     /locus_tag="IXH60_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1990900.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="IXH60_001993"
     CDS             complement(7199..7576)
                     /locus_tag="IXH60_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1990901.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="IXH60_001994"
     CDS             7800..8993
                     /locus_tag="IXH60_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1990902.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="IXH60_001995"
     CDS             complement(9159..9581)
                     /locus_tag="IXH60_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1990903.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2891 a         1934 c         1590 g         3274 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctc
//
LOCUS       DACEZN010000104         9500 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEZN010000104 DACEZN010000000
VERSION     DACEZN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.104"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..224)
                     /locus_tag="IXH60_001996"
     CDS             complement(<1..224)
                     /locus_tag="IXH60_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1990904.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDA"
     gene            complement(372..2606)
                     /locus_tag="IXH60_001997"
     CDS             complement(372..2606)
                     /locus_tag="IXH60_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1990905.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3085..3351
                     /locus_tag="IXH60_001998"
     CDS             3085..3351
                     /locus_tag="IXH60_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1990906.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3351..5078
                     /gene="ptsP"
                     /locus_tag="IXH60_001999"
     CDS             3351..5078
                     /gene="ptsP"
                     /locus_tag="IXH60_001999"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1990907.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5209..6288
                     /locus_tag="IXH60_002000"
     CDS             5209..6288
                     /locus_tag="IXH60_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1990908.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            6305..7528
                     /locus_tag="IXH60_002001"
     CDS             6305..7528
                     /locus_tag="IXH60_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1990909.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7617..8504
                     /locus_tag="IXH60_002002"
     CDS             7617..8504
                     /locus_tag="IXH60_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1990910.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8590..8820
                     /locus_tag="IXH60_002003"
     CDS             8590..8820
                     /locus_tag="IXH60_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1990911.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(8877..>9500)
                     /locus_tag="IXH60_002004"
     CDS             complement(8877..>9500)
                     /locus_tag="IXH60_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HAQ1990912.1"
                     /translation="TARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPD
                     LQEMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFI
                     RRIQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKE
                     AHLSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3030 a         1686 c         1942 g         2842 t
ORIGIN      
        1 gcgtctttga ttttatctaa gaaataattt ttagcatctt cgatttcttt cggctggatt
       61 ttacgtttgt cattgatcag cagaatttca ttatgttcag gggcttcagt gtagacaata
      121 gtggtatttc cggctgtgta gaccttgact agatgggcat cagtgttttc aagctggttt
      181 aatacaagac gggaatggct gtttgttaca tttaccaatt tcatggcgct cacctctttt
      241 atgtattttt aattgcttgt ttctcttatt ataatttctt ttttataaaa tgaacagtat
      301 tttgactttt ctgaaaaaag aatgagacgg catttctcaa agaaataccg tctcacgcta
      361 taaaactgga tttattcagc cgtttcttcg gctgattctt tagccaaacg ttctggttta
      421 gaagtaacga tgatttttcc atctttatcc agttttgctt tcaactcatg gatagaaggg
      481 tgatcaagat aaaattcagc gataccatct tcgatttgtt cttggatcgt gcgtcgaaga
      541 ggacgagcac ccatagatgg atcatagcct aagtcaacta atttttcttt gacattcgta
      601 ggaacttcga tgtgaagttg ctgtgctgcc agcatttggt tgacatcgtc tagcattagt
      661 gtgacgattg tcatcaagtt ttcttttgat aatgcactga attcgatgat cccgtcaaaa
      721 cggttcagaa attctggtgt aaagtagttg tttaattggt tcaatacaga acgagtaaca
      781 ccttcgcgtg ccgcaccaaa accaacgttt gcttctactt taccagttcc ggcattactt
      841 gtcataatga tgatcgtatc tttaaagctg accgttcttc cttgtgcatc tgtcaatcga
      901 ccatcatcta agatttgcaa gaacatatgc aaaacatctg gatgagcttt ttctacttca
      961 tctaacaaga caagactgta aggatttctc cgtacttttt ctgttaattg accagcttct
     1021 tcgtatccga cataacctgg aggagaacca atcaatttag agacactatg tttttccata
     1081 tattcagaca tatcgaaacg aatcattgaa tcttgtgaac cgaatagttc gtaggcaagt
     1141 tgtttcgcaa gctctgtttt acctacacca gttggtccaa caaataagaa ggagccgatt
     1201 ggacgattct gtttgtttaa tccaacacgg tttcggcgaa tcgctttcgc tactcgatct
     1261 acagcgttat cttgtccgat gacatgagct ttcaagtcat ctgctaagtt tttcagttgt
     1321 gtctgttctt tttcttttaa ttcacccact ggaatacctg tccgttgttc aacgattttt
     1381 tccatatctt tttcggtaat cacaggtgtt tcctcttcag tgagctgacg ttctttcatt
     1441 ttttgaagtt tattgatctg atcgcgataa taagctgctt tttcgaaatc ttcttctcga
     1501 gaagctaaaa ctttttgttc ttctgcttct tgcagttttt tctcgattgt tttaggatca
     1561 acgatttgga tcgtcaagtt tttcttagaa cctgtttcat ctagcaaatc gatggcttta
     1621 tccggcaaga agcgatcttg gacgtaacga ttggataaag ttgcggctgc ttcgatggcc
     1681 tcatcagtat attttacatg atgatagtct tcataacgtt tttgcaaacc tttcaaaata
     1741 ctgatcgttt cttcgacagt tggttcatct acccgtacag gttgcatacg acgttctaaa
     1801 gcagcgtctt tttcgatgat gcggtactca tttaaggtag tagcaccaac catttgcaat
     1861 tctcctcgag ctaaagctgg ttttaaaata ttaccagcat ccatatttcc atcaccagca
     1921 gaaccagcac cgactatttc gtgaacttca tctatgaata ggatcacatt ttctgcttga
     1981 cggatctctt caatcaattt ttgcatacgt tcttcaaatt gtcctcgtat accagtacct
     2041 tggactaaag atacgacatc taaacgaatg acttctttgt ccattaattt ttgcggtaca
     2101 tcaccatcta cgattttttg tgctaaacct tcaacaactg ctgttttacc aacacctggt
     2161 tctccaatca agacaggatt gttcttggtt cggcgattta aaatttcaat gactcgtttg
     2221 atttcgtcat cgcgtccgat cacaggatcg atatcacctt gtcgtgcttg ttctgtgata
     2281 ttgattccgt attctcccaa taaaccttct gtattgcctt gaggtggctg accaccattg
     2341 aagttgttgt ttccgccaaa ttgtgttggt ggtgtttgct cataatttcc ttgctgttgc
     2401 atttggcgag acatggaacg gaaaagatcg tccaaactgc caaatccaaa tggatcattt
     2461 tgggccatat tagtcagacc tccattttgt tgatttttga ttttttgata acagctctga
     2521 cagtaatcga gttgtgtccg ctgaccattg acagtggcat acaagtgaat cgttgcctca
     2581 ttcttgccgc agttttgaca aagcatgaat cattcacgtc ctttcacaaa aatgggtaca
     2641 tccatatttt acatctcgtt tttcatgagg taaaagaata acccctagtt ttttaagaga
     2701 tttatgaggt agaaaaggtg aaatctctat tgaccattcc tgacctttga ctcttattat
     2761 actaaaaata aaaagggaag caagtaattc gtttcatatg aaaacaaaga gggtcaaagg
     2821 aaagaatgat aaacgtcagg agaagagaaa gcgacaagaa aaaggaaata aacacgtttg
     2881 ttataaaatg tgaagaaaag gatgaaaaag aatccggaat aatttgaata cttttatctg
     2941 aaattgaatg aaaactgttg tatctaacga aaaaaaatgt taatctttac agatgaaagg
     3001 gtggcatttg ttaggattct ctgaattact aaaaaagaaa accctcacat aaaaacaaca
     3061 ctcacttaaa ggagaccgat taatatggaa aagaaagaat ttcacgtagt agcagaaact
     3121 gggatccacg ctcgtccagc tacactatta gtacaaacag caagcaaatt taactctgat
     3181 gtaaacttag agtataaagg taaatcagta aacttgaaat caatcatggg cgttatgtct
     3241 ttaggtgtag gtcaaggttc tgatgttact attactgcag aaggtgctga cgaagctgat
     3301 gcaatggcag caatcgttga aacaatgaag aaagaaggct tatctgaata atggttgaaa
     3361 tgctaaaagg gattgccgct agtgacggtg tagccgttgc aaaagcttac ctgctagttc
     3421 aaccggattt aacattcagt aaggcaacag tagaagatac tgcggctgaa gaagctcgtt
     3481 tggatgctgc attagctaaa tctactgaag aattgcagca aattcgtgaa aaagcagcgc
     3541 aaagcttagg tgaagcagaa gcgcaagtat ttgatgccca tttgatggtt ctttcagatc
     3601 cagaaatggt aggtcaaatc aagcaaaata ttaaagacaa cagcgtaaat gctgaatcag
     3661 cgctaaaaga agtaacggat atgtatatcg gtatgttcga agcaatggaa gataatgctt
     3721 atatgcaaga gcgtgctgca gatattcgtg acgttgctaa acgtatttta gcgcacttat
     3781 taggcgttac tttaccaaat ccatcaatga tcaacgaaga agtggtcgtt gttgcacatg
     3841 acttgactcc aagtgataca gcacaattag atcgcaattt tgtaaaagcg tttgtaacag
     3901 acattggtgg acgtacttca cattctgcta tcatggcacg ttctttagaa attccagcaa
     3961 tcgttgggac aaaagaaatt actgctaaag tcaaagaagg cgatattttg gctgtcaacg
     4021 ggatcgaagg cgatgtaatc atcgatccta cagatgaaca aaaagctgaa tttgaaaaag
     4081 caggtgcaga ttacgcagca caaaaagcag aatgggaaaa attaaaaaat gcagaaactg
     4141 tcacagctga cggcaagcat tttgaattag ctgcaaacat cggaacacca aaagatctag
     4201 ttggtgtcca taataatggt ggagaagcag ttggtctgta ccgtactgaa ttcctgtata
     4261 tggattctcc agacttccca acagaagatg accaatatga agcttacaaa gctgtattag
     4321 aaggtatgga aggaaaacct gtagttgttc gtacaatgga catcggtgga gataaagaac
     4381 taccatatct gcaattacct catgaaatga atccattctt aggatatcgt gcattgcgta
     4441 tcagtttatc tgaacaaggc gacgaaatgt tccgtacaca aatgcgtgca ttgcttcgtg
     4501 catctgttca tggtaattta cgtatcatgt tccctatggt agctacactc aaagaattcc
     4561 gcgcagcaaa agcgatcttt gaagaagaaa aacaaaaatt gataagtgaa ggcaaggaag
     4621 tatctgatac gatccaagtc ggcatcatga tcgagattcc agcagcagca gtcttagcag
     4681 acaagtttgc aaaagaagta gatttcttct ctgtaggtac gaacgatttg attcaataca
     4741 caatggcagc agaccgtatg aacgaacgcg tttcttactt gtaccaacca tataacccat
     4801 caattttacg tttgatcaaa aatgtaattg atgcagcaca cgctgaagga aaatgggcag
     4861 gtatgtgtgg agaaatggct ggagatcaaa cagctgttcc attgctagta ggtatgggtc
     4921 tagatgagtt ctcaatgagt gcgacatcta ttcttaaaac gcgtagttta atgaaacgct
     4981 tggacacagc gaaaatggct gaacttgctg atcgtgcatt gaaagaatgc gatacgatgg
     5041 aagaagtcgt tgaacttgtt catgaatatg taaaataaac aacgtatcaa aagctggtgt
     5101 ggcttgtaaa agagctacgc cagttttttt gttctttttt aagaaaacgt ttttgtcagt
     5161 aagaatagac aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat
     5221 ataagtgaag ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt
     5281 gcaaaatctg gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt
     5341 atctcttata cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga
     5401 atcaatagtc ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat
     5461 gcgcattcta cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct
     5521 atcgtaaaaa aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca
     5581 ccttattcaa aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc
     5641 aatgggatcg acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa
     5701 tatttcaaac tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt
     5761 aaaggaatca ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg
     5821 tttggtcatg ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat
     5881 catcctgaaa atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat
     5941 tcaggtgcag atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta
     6001 gaagctttag caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg
     6061 ttagaagata gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc
     6121 caacagatta ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa
     6181 gagcgaagta tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat
     6241 gaaaaagtct ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa
     6301 aaacatgaaa atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac
     6361 atcgatcaaa acattaaaac aagaattaga aagacatggg catgaagtct atatttttac
     6421 aacgaccgat cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc
     6481 atttatttct ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt
     6541 tgccaaagaa ctagaactag atcttattca tacccataca gaattcggag cagggatact
     6601 tgggaagatg gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta
     6661 tgaagactac cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata
     6721 cttgtctaga ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat
     6781 tgatacatta cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga
     6841 cattgaaaaa ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc
     6901 actaggtctt caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa
     6961 aaatatccaa gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag
     7021 attagttatc gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact
     7081 gcagttagaa gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta
     7141 ttataaagcg gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta
     7201 tacagaagcg atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg
     7261 attgttcgat catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc
     7321 acttattgat tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact
     7381 ttatgatatt tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt
     7441 tgtttatcat gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa
     7501 aataaaatta aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt
     7561 ttctagcgta tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga
     7621 aacttggttt tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag
     7681 caggacacga agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag
     7741 aaggagctat ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta
     7801 caatggtagg ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt
     7861 ctgcagatat ctcaggacat attttagttg atttgactac aagtacacca agcctggcag
     7921 aaaaaattgc aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg
     7981 gtggtgactt aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg
     8041 tttatgatca agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt
     8101 ctgctgggaa aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga
     8161 caggaatgac ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga
     8221 ttgagacgct ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta
     8281 ttttaaaaga agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga
     8341 ttgcattaga agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga
     8401 aactatatga agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca
     8461 aactttggtg gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta
     8521 gagaaaatca atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga
     8581 ggacctccga tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc
     8641 gagtcagcag ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt
     8701 acagtaactt atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag
     8761 caatacgaat ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa
     8821 attttaggat aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa
     8881 tttagttgct tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct
     8941 aattttttgc ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga
     9001 cgtttcaaag ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca
     9061 gaggtaatca ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc
     9121 tcatctgctg ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt
     9181 tcctggatcc gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct
     9241 ttttcgaaat tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc
     9301 tttttatttc ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt
     9361 tcctgtaggt caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc
     9421 atttgataag tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa
     9481 ggcccgtatg ttcgagccgt
//
LOCUS       DACEZN010000105         9317 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEZN010000105 DACEZN010000000
VERSION     DACEZN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.105"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            14..2140
                     /locus_tag="IXH60_002005"
     CDS             14..2140
                     /locus_tag="IXH60_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990913.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            2137..2757
                     /locus_tag="IXH60_002006"
     CDS             2137..2757
                     /locus_tag="IXH60_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990914.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            3450..3830
                     /locus_tag="IXH60_002007"
     CDS             3450..3830
                     /locus_tag="IXH60_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1990915.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3812..4726
                     /locus_tag="IXH60_002008"
     CDS             3812..4726
                     /locus_tag="IXH60_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1990916.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4966..5655
                     /locus_tag="IXH60_002009"
     CDS             4966..5655
                     /locus_tag="IXH60_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990917.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5776..5862)
                     /locus_tag="IXH60_002010"
     CDS             complement(5776..5862)
                     /locus_tag="IXH60_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1990918.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6507..7244
                     /locus_tag="IXH60_002011"
     CDS             6507..7244
                     /locus_tag="IXH60_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1990919.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7272..7811
                     /locus_tag="IXH60_002012"
     CDS             7272..7811
                     /locus_tag="IXH60_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1990920.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(8309..8770)
                     /locus_tag="IXH60_002013"
     CDS             complement(8309..8770)
                     /locus_tag="IXH60_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1990921.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            8903..9277
                     /locus_tag="IXH60_002014"
     CDS             8903..9277
                     /locus_tag="IXH60_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1990922.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3597 a         1414 c         1454 g         2852 t
ORIGIN      
        1 ggaaattcaa aatatgttaa aaaaaataaa cgctgtttta tcactttttg tctgcattac
       61 aatactattt ttttctacta atgttataaa tggtataaag acttctgaag cgctatataa
      121 aaataccgct gaaattcata tgcaatataa agatgatatg gactttaaca gttatataaa
      181 cgctttgtat gaaatatcta cacaagaaaa tattaatatt tctcaatata gttttactgc
      241 tgttaaccaa ctaactattc ttgctacaaa ccctaacgct aacaaacact ggaaattttc
      301 taaaaaaaat atattagcaa gaaacgattc taaagtccat tatactaata acaaaaattc
      361 taagtaccaa ttaaaacttc caaataattt tttaaatata aatatttatc ctttttctca
      421 tgtggaaaat attggattaa gtgaaatatt atatgtacaa ggaaactcaa acaaattaat
      481 tcctgtactg aaaaaatatg gaacacctac tatttccaat atcaatcaat cagatacttt
      541 tcaaattaat actaatattg ttcttgttat ctgctatctt agtatattta ttgtaattac
      601 tacaattata tttgcttttt caaaaatgaa agaaatcact ctaaaaaaaa tgttaggcta
      661 taactctttt gatactataa aagattctat taaagagtta aacctcttat tttgttcttt
      721 gattataact ttcattttat ctattttttt gagcatatat tacagtaacg aaaatttttt
      781 cctatcattt cttctatgta gtatttttca ttttataatt agttttttat ttatcatagt
      841 tactctaagt atctatcatt tatctagtat aaaaaattat ctaaaaaatt cacgaccact
      901 taagttaaat ctttgtatat taaatatctg tctattctta agcatgatcc ttctgttaat
      961 agcttctaca aaagtaataa atacccataa tgaagccgaa aataattctc ttaaatattg
     1021 ggaaagaact actaatttat acaaaacaaa tattactaat caattaaata gaaacaacac
     1081 cgtagaagaa aataactact tgaaaaaagc tagtaaattt gtttataaaa tacagaagaa
     1141 ttataaaact tttattatcg cgccatacaa ttacgcaact atccaagaga ataataaaga
     1201 attttttatt ggacaaaaag tttatccaaa ttttgaagac tatgttagtc aacctgctgg
     1261 tgcaggaata tgtgttgatt taaactatct aaaatattac aatcctattt cttttgaaga
     1321 atctactgac ttaaatttaa taaactctga tccactaact acttacattc tagtacctaa
     1381 aaaatataaa gaatatgcaa aaatgattga aaaaaattat ttagaagaca tacagtttag
     1441 acttgaggac tctcctcctc aagctcctta taaaaatcct aatttgaaaa atttaaaaat
     1501 aaaactaata tttgttaata ataaccaaaa gtattttagt tttaatacct tatatggaaa
     1561 ggcaaaagat aactatacaa taactgatcc catagttaga gttattaatc cagaaataac
     1621 agaatcatta ttttggggaa atatattgac tagtgatggt ggattatttt ttgatcagaa
     1681 acatacttca aaccaaaact tttttaatca aattaatccg tatataaaag aatttaacct
     1741 agaaaatatc ataaattttt cttattctgt ttttcaagaa actggtgaat tattatctca
     1801 aaaaaaagta aataaattaa atgcatatct acaatatctt ttaatattag gattatttat
     1861 aataattaat tttcaaatga tttttgttag ttttactata aattcaaaaa aaatagcttt
     1921 aaaaaaaata tttggatatt caccaataag tcaagtttta gatttatgtt tactcccttt
     1981 tacaatagag ttatttgcta ctatgtgttt tcaattgata ttttcaaaaa acacttactt
     2041 gtattttata atttttatga tctttttaat aaatctaatt acttctatta ttatttataa
     2101 aaaaaatagt tttaaatatt ttaaggagaa tattttatga tattatcagt tgagaatttc
     2161 accctgtcaa ttaataaaaa aaagatcaaa caaaattgga acttttcaat atcagaaaaa
     2221 gattttatta tagtaacagg aaaaagtggt agtggaaaaa ctactctgtt aaataattta
     2281 agcctaatag ataaacaata taccggtaac ttattctatt ttgaaaagaa agtttctaaa
     2341 catgatatac aacgtctacg aagaaatcat attagctatt tatttcaaaa ctatgctctt
     2401 cttgaaaatc aaacagtaca atacaatttc gaattagcaa aaaagtttaa cagtaatatt
     2461 tccaatgatc atatttataa tttattaaca gatttttctt tgccaaaaga tatcttaaaa
     2521 gaaaaaatct ttctcttaag cggtggagaa caacaaagaa tagcattaat tagaaatatg
     2581 ttaaaaccat ttgatatatt atttgcagat gaacctacag gaaatttaga taaagaaaac
     2641 tcaactttta taatgaactt tctgaaaaat ttaacagaaa aagaaaaaaa agccgttgta
     2701 ctagtaacac atgacttaaa tgttctaaac tatggaacta atattataga attataattt
     2761 ttacccgtga tgctagctga tttaaaacta gcaccacggg tatttatttt atattccatc
     2821 tctcctatcc caaaatttaa ataatcactt aataaaaagg aggcacccaa gtgattttat
     2881 tgaaaaaaga gtatgaaaaa ttttatgata gacttattta ttctttgcaa ttgccaaaaa
     2941 aattaaaccg tcaattacaa aagacttatg aaactaaatc aactgaagaa tgggagagtt
     3001 attgctagat ttttggtaga agtaacaaaa agtggacata ttatcataat gactcgccga
     3061 aagtctgata aaaaattggc taaaaatatt gctgaatgtt taataaaact atcagtttta
     3121 gctaaatagt tagtagttta cacaaaaaag catttatgtt ttttgttgca aagcaacaat
     3181 ttcatcttcg gtgaaataag cgaaacttta caaaaagtgg ctagcatttg ctagcttttt
     3241 ttgtattgtg gagcgtggct tttgaggggg attcaagggg gagcttgcgc ccctttgaca
     3301 agcggttatt tgcactaaat acgtaggtat ttggctagca aatatagagc ttgccaaacc
     3361 cttttagtga gctttactca ttgtaccttt ttaggtacaa tggtattgcg aacgatacgt
     3421 ccgtaggacg tactgaaagg gtgggactta tgagcgaaca aaatcaaaat ttggctagca
     3481 attcttctaa aaaatatacc tatcgatccg agccaaaaca aatcagtttt cgagtgagcg
     3541 aatccgaatt tgcaaagctg aagcaatcgg ctgaagcttt gcaaatgagt gtgccggctt
     3601 tcgtgaaagc caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg ccagacattg
     3661 cccaggcaat cgcgcgcgat ttagccaaag ctggagggaa tatcaatcaa attgctaagt
     3721 ggtgcaatac acaccaacat gacgttgctt ctggtgacgc tcaacgttta tccgaaaacc
     3781 taaaaatcat gcaaaaggag ctacaaaaga tatggcaaca attaaggtaa gccgaaccac
     3841 ttcttgtagt cgtgcaatta attacgcaga accacgagca accattaaaa caggaataaa
     3901 ctgtgacgtc gattatgcca aaagtgaaat gaaacaaatt cgcatgctgt atggaaaaga
     3961 cgaccgtgta caggcacact tattgattca atcttttcga ccaggagaaa ttaccgctga
     4021 aaaagctaat caactagggg cagagtatgc aaaaaaaata gcccccgatc accaaatagc
     4081 tatttacaca catacagaca aagaccatat tcataatcac attgtcatta actccgttaa
     4141 tctggagact ggaaaaaaat ttcaagctca tggtcaagca tttctaaaca aatgttatga
     4201 cctaaatgac gagatttgtc ttgcacataa cttaccaatc actgagcgag gaaagaaacc
     4261 agaaaaacga accatgtctg aaataaaatt aaaagaaaaa aatgaacctg tttggaaaga
     4321 cgaaattcgg attgcgatcg atcaaacaat gaaaaacaaa aaaaccagaa cctatgacca
     4381 attttgtaat tccctcaaaa cattggggat tcattgcttc aatcgcggga aaaatttcac
     4441 ttacgaattg atccacaaaa agaaaaaagt acgatccaac agactaggaa aggactacga
     4501 aaaggagacg attttacatg agctggacag acgagaacga acctataacc aacagcgaac
     4561 caacgagcta cgaagagctc gagctagcat tcgcgaaact gcagaagaag tacgaagaga
     4621 acaacgaact cttacgcctg aacaaccgga aattagaccg gctcgaagaa accatgaaca
     4681 acacgttgaa agaggaacag accaccaaca agaattatcg ctctaaaacg ttaaaagaca
     4741 tagaaaacga aataaagaaa caacgtgctt ttctcgttcc ttatacgcaa gaaaggatac
     4801 aaactacgat tgatttacaa gttaaaaaac tccatcaaga gctattagac cagcgaaata
     4861 aggaacagaa aaaggtgctt attcaacgat cattggattt tcttgccccc ttatgttgct
     4921 tagtcctgac actgttcgca ttttggttag tatggggagg ctaaaatggc attttattta
     4981 ccctatctac tcatttttgt tagtatttca ggaagtattt ggctcatcta taaaattttt
     5041 caaacaagat attcattaaa aggctctaaa attcgattta agcgattttt ctttcttggc
     5101 tgtatatttt ccttgataat cgtttcaagc ggcttattcg gcgttttaga gggaaataag
     5161 cgcgtttcta gaagtatttt attaggaaat gtcactcaaa agtatgaatc agcgcgtgat
     5221 aaaaagaaaa aagagcaagc attagcacaa aaaatggaag agtttaccac ttgttatgag
     5281 gaaatgaacg atatttttgt gaatcaagaa aaacgtttga cggataaaaa catggaaaaa
     5341 ctcactcgtc tttatcaaaa cttgccagaa aaacagcaaa aagaggttca agacaactat
     5401 gagcgaacca aaaaagacgt gcaatatgta aaagatacaa agatagaaga agcctgtagt
     5461 gatctatttg gagacacaaa cccttggttt gccagtgaag aagaaaaaaa agaaaaacaa
     5521 caatctgtca catatgaacg ctatgaaatc ttacttcaac aagcaactaa catgcaaaat
     5581 ccgacaaaaa aagaaaccgc cttaaactat ctaaaaagtg tcaaggaatg gttagatcag
     5641 caacaacaaa attgaatttg atttaaaatc atgttaaact gaatgtgcaa atgatcaaac
     5701 attagataca cgaaagcccc agagaggtcg gattctctgg ggcttttttt caggttgatg
     5761 tcgcctttat tgattttact tcttgtttcg gttattcaac caataactga ataaagagac
     5821 acaaactcct acaagaaggg ggcaaatgaa cagttcaaac attagataca cctccttcca
     5881 gtcgcaagac tgtcggatag gcgacaagtt attatatctt aaaaaattaa atttgtgtgg
     5941 tgtttttaag tatcacttgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     6001 cttctcgact agattaaggg aaattaatca agaattgtct tagaacggct tagacaagct
     6061 ttcagagtct cacacctcct ctttgaacaa cggttttagc aaaggagccg gaggcgactg
     6121 cgcagtataa tatgagagca tagcgatcac acctttttag cgaccgtagg gagcaagttt
     6181 tccagtttag aaaaattcgc gaaccttttt ggaggaaaaa gtaggagttt tttaggcggg
     6241 aaattcaatg tgctcttaag atatactgtt agatatactg ttagatatac tgttaataat
     6301 aactgttagg gattagcaac cttatgggag agtaagtatt gacataggta gtgggggata
     6361 aattgtcaca ctatggggga taaattgtca cactatgggg gataaattat cacactatgg
     6421 gggataaatt gtcacactat gggggataaa ttgtgtttta catcctctta tgtctatgat
     6481 aacataattt attgaggtga tttttattga gtaacgatat agttcgatat aataatggat
     6541 taaattctgt ccctttaaga aaatttaccc ctatagatat ggatttattt tggggaatat
     6601 gttctaaaat gaaaagaaaa ggtacaaaag aagaaatttt tacatttaaa caaataaaag
     6661 aattaattga ttacaatata aatgcacgtg gcgaccgttt tgctaatgat ttaataaaaa
     6721 tgtcaaaaaa aatcattact ttgtctttta tttatgaaga cgaaaaaaca tatgaacaat
     6781 taaatctgtt tcaaaaattt aaaatcgata aagtgaatga aacattatta ataaaggttt
     6841 ctgaagaatt tgaatatata cttaattcaa tcggaactaa ttttactcgt ttcgaattag
     6901 aaaatatgac aaacttaaag agtagctacg ttaaagagtt atatcgccaa cttatggctc
     6961 ataaagacag aagatctaaa aaaggtcact ggtttgtaaa aatagaagat tttagagctc
     7021 tattagcaat accagaaagt tatcgcatga gagatattga taaacaaatt tttgcccaag
     7081 cgaaaaaaga atttttaaac gttaattcaa ctcaccctgc tatttttaaa acttttgaaa
     7141 tcaaaaaaat taaagcctac aaaggaaata agataggatc tctgcaaatt tattttgaag
     7201 aaaatatacc ttcaattacc ttagataatt ggttagagaa ataataccta acaatttagg
     7261 aggcaattat catgcaaccc aaagaaacct ttacaataaa ggaattatct gaattatttg
     7321 aaataagtag gcaagcaatc tctaaacatg ttgataaatt agacccgtcc ttattagcta
     7381 aaaatgagcg tggttataag gttgtattac gttctggagt attgcaactt gctagcaact
     7441 taggtaacca aaggttacta tataggttgc atgagtatga acaagaaaac gataaaaacg
     7501 tttatatatc aagcaaaaac gtgattgtaa acgagttgct agataagttg cgtgaagaaa
     7561 atgaattttt acgagaacaa ctttccctca aagagcaaaa taattctaat atgcaaaaat
     7621 tactcgacca gcaacaacag ctcaccctac aggcaaatca acagatcgaa aaactgcaag
     7681 aacagttaca attagcttat acggaagaaa ctccagaaga tgaatcaact actattcttt
     7741 ctgaagaaac agaatcgata gaaaaacaca caaaaaagaa gaagcaaaaa tggtggcaac
     7801 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     7861 tactaggaat tctaggcttc agtccgatgt tattgtcagt aatcaataaa gggaataaac
     7921 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     7981 aagagacgga taaataataa aaaagactgc tcttccgagc agccagttaa aaaataatga
     8041 gtgtttattc ttgaaaaagc acgcgcttat gaagcgctac caaaaattta ccatatttta
     8101 caaaaaataa caagtcttaa aaaaatgaca gataaaaatg cacaaagaat tagatataga
     8161 taaccgcgaa aaatcaagct ttagaaagtt gcttttagct agaccaatta aaaatcacaa
     8221 agtgagcgtc tctatccatt ggtagagggg ctacttggca tagtgtacat tatgtcaact
     8281 atgcaattaa ataactaact acttcatatt agcttaataa aataatcaaa ataactaggg
     8341 ggaaaaacat tgtataaaaa aatttttcat tttttaaata agaatagtaa atataaaaat
     8401 aatttaagat atctattact attaatccag atagagcaaa ccaagtaaat attccatgtg
     8461 taaagctaaa aaaattcatt tgaatcaatt caaataaacc taatatagta acaaatatat
     8521 taaaaataat agctgtcaat accagtaaat tatttttttt attttttgaa ttatcttcat
     8581 ttaaaataga atttatgtta gtactataaa attcacaaat tttttttagt gtctctatat
     8641 ctggtgttgt tttgttattt tcccactttg aaatagtttg acgtgtaacg ttaaattttc
     8701 ttgccacata ttcttgagta tacccttttt tcactcttaa ttctcttagc ttagatccta
     8761 aatcctccat tttttctcct atgtaaacga ttatttacat aaaattttat cataaaaata
     8821 ctaacgcgcc aaaaaagttc tttattttat aaaacacact aaaataaaat aattctagta
     8881 aaaaagatag gagaaataag ttatgaaaaa atttttatgt ctttctgctt taatgggtgt
     8941 cttattaact agtggaggaa tagctagtgc cacagaagct ttaaatctag atgtacccga
     9001 agaccatacc gccatttacg gaggaggaat ggaagctaca gaagaaggat tttcttctag
     9061 taaattgaga tatgctgctg gcggcgggga tttcaactgt ggggttaatg gatttaaagt
     9121 ttatgcaaat tactaccatg ctagagctaa acatagtgca acagctaaaa atggacgagg
     9181 cggtcaagta cgatcagttc agaaagctgg agtaagagct tatgcaacat gcaatgccac
     9241 acttactgga aatactggtt ggtggaatgt ttattaaaaa agagagttta aactctcttt
     9301 tttatttaca tttgaaa
//
LOCUS       DACEZN010000106         9313 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEZN010000106 DACEZN010000000
VERSION     DACEZN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.106"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            6..980
                     /locus_tag="IXH60_002015"
     CDS             6..980
                     /locus_tag="IXH60_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1990923.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1710..2027
                     /locus_tag="IXH60_002016"
     CDS             1710..2027
                     /locus_tag="IXH60_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990924.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2231..2596)
                     /locus_tag="IXH60_002017"
     CDS             complement(2231..2596)
                     /locus_tag="IXH60_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990925.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2804..3286
                     /locus_tag="IXH60_002018"
     CDS             2804..3286
                     /locus_tag="IXH60_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990926.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3483..4373
                     /gene="pstS"
                     /locus_tag="IXH60_002019"
     CDS             3483..4373
                     /gene="pstS"
                     /locus_tag="IXH60_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1990927.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            4698..7169
                     /locus_tag="IXH60_002020"
     CDS             4698..7169
                     /locus_tag="IXH60_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1990928.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7272..9008)
                     /locus_tag="IXH60_002021"
     CDS             complement(7272..9008)
                     /locus_tag="IXH60_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1990929.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9005..>9313)
                     /locus_tag="IXH60_002022"
     CDS             complement(9005..>9313)
                     /locus_tag="IXH60_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1990930.1"
                     /translation="EYLSIGQITADLTNYQVTVDDHLIELTPKEFELLVYFMKRKDRV
                     IDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKYLVTIRGFGYKFQEPKR
                     "
BASE COUNT         3046 a         1559 c         1794 g         2914 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatatta tagattacaa atagtatcgt
     1441 atttcgcaat cttaaaatta tcttcttact atgaataaga taataaaatt tgtatttgtc
     1501 ctttttaggt aacttatttt aatctatata cctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatggt aactatgcac tgtaacgata atgctgctaa aatatctttt
     1621 tacgaaaaac atcacaagga aaaacttaac tatggtatta tctaaacata attttttcgg
     1681 tatttaatat ttaattagga gggaatattt tgctttttta tagtaatttt atcttaatag
     1741 tagcgatatt attacttctg aatatatgga tttttgatag gagtagaaat gcaagtatag
     1801 gttttagaac taaacgaagt ctgtcttcta aaaaaaattg ggtatactca caaactattt
     1861 tttatggagg aattgtttta attagtttac tttcttcaac actttattct ttgaatatta
     1921 tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct gcaattatta
     1981 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
     2041 agttttggtg atagtgatat taaaaatcag aaaagataga aaaagcagga gatagataaa
     2101 atgctgatat ttcagcattt tttaagagta taaaagagaa aaaaagtaat aaatgtatac
     2161 atgatataag aaaaacatat atgtataaaa agaagaaagt agcttctctt tgtacatata
     2221 tggtttgttt ttatggatgc cgagcttttt tataagataa ggctaccgcc cataacgacc
     2281 caaggatttg tggaactaag taaatacctg caagaagcaa gatcagctgg gtcggtttta
     2341 aaggatgata gaagtaacgc cattcagtaa aattggcgca aaataaatag gaagtgacag
     2401 tagatataat attggcaatg aataaagata taatacgcct gttcctttga aaataatatc
     2461 cagcaaaaag agataaaaat atcaatgtga tgaacccgct tacggaatga taacgaaaat
     2521 caagcagtaa ggcgaaatgc acataaggaa caaaagataa aagcaacgga aggtttttcc
     2581 agcaagtttt tatcatcttt catcaccacc taacaagtgt tattattttg ttagttttat
     2641 tgtaaattga atcctgcaat aatcaattac tttttcatta tttctagatt aaatgaataa
     2701 actctcatgc cattatctct aaaaaaaaag tttttccccc taattttttt agatagaaaa
     2761 ttgtatagta tgatgagaga aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt
     2821 aatcgatcgg ctttatttat ttttttggtt ttttcctatc gttttgctac cttttacatg
     2881 ggtaaatgct gttgtacaag gtgcggagat attaggcctg atccaaggaa gcggatttgt
     2941 ggtttctagg aattttccag ctttgactgt tttctattta gtgataagtg taggtctctt
     3001 attaccaaaa tctctgatca atcactacaa ttggcagctg ctttactttt cttgcttatt
     3061 agtatttaca tctatgtttt ctttgctgcc tcgcttgatt atcggcccta atggtttgag
     3121 cgagagtgga ttgaaggatt cagcttattc ctccatgtat ttcttgacga tcaaaccatg
     3181 gttctattta gtaattgcct catttattct tgcaacattt ttttacttct ggacagaaat
     3241 caggataaaa agtgaacgat ctgtatctat aaaaaataac tattgatggt ggatcatcag
     3301 taaaaaagca tatgagctgc gtaaaatgca tgtttctcat atgctttttt tattattatg
     3361 taaagaatgc gaaaaacgtt tacatgattt ttacataact taactatatt tttacagcaa
     3421 ccatacgagg gatttacagt cgactgataa actttagttg taaataagaa aggagcaata
     3481 aaatgaaaaa agtattgttt actctaggta tgatgggaat tcttttagcc ggatgcggct
     3541 caggatcagg aaatacagat ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac
     3601 aagtaaaaat cgttgcggta ggttcgacag cattacagcc gttagtcgat gcagcacaag
     3661 aaagctttgt acaagaaaat ccaaattatc aaatctctgt acaaggagga ggaagcggaa
     3721 ctggtttgag ccaagtcgaa gctggagcag ttactatcgg aaactctgat gtatttgcag
     3781 aagaaagaga cggagttgat gcttcaaaat tagtggatca taaagtagca gtggtcggga
     3841 tggcgccaat cgtcaataaa gatacagatg tcaaagatat caccaaacag gaattgattg
     3901 atatctttac aggaaaaatc actaactgga aggaagttgg cgggaaagat caaaaaatca
     3961 acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac attcgaaaaa tggggactgg
     4021 atggtgctac ccctgtgcag tcccaagaac aagattcatc aggaacagtt cggcagctgg
     4081 taagtcaaac accaggagca atcagctatt tagctttctc atatctcgat gattccacac
     4141 aagcactaag tatcgacggt gtagaaccaa aagaagagaa tgtagcagat aacagctggg
     4201 gaatctggtc atatgaacat atgtacacga acggaaaacc ttcccctgaa gtccaaaaat
     4261 tcttagacta catgatgaca gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt
     4321 taccaatcac aatgatggaa gtagaacgtg atcacgaagg aaatatcaaa taactagcag
     4381 ataaacatgc cacggtagtc gttactatta aggaaaaatc aactatgttt cccacatata
     4441 catttttcct ttaattgtga aaaatagttc ctcatatttt gccgtttttt gagctaatac
     4501 cgagggtcag ctgattaaac accgtttatt cgaaaaaaag agctacgaca tagaactgtc
     4561 gtagctcttt ttttgctata tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa
     4621 cactttccat atctgttcta tactggatag gagaagtttg gaaatatcca gaaaacgaaa
     4681 tcaatcgagg tagccacatg aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa
     4741 cacaaagatc atcagataaa gtcatccgtc tttttccatc gtataaagaa ggattaacgg
     4801 cagatcaagt caaggaacga atagaaaaag gtgcagcaaa taattcagtc gatcccacat
     4861 tcaaaacaaa tcagcaaatc gtattggaaa acatatttac gtatttcaat ttgatctttc
     4921 ttatcttagc cattttgctt tgtttagtag aatcctataa gaacttgact tttttaccag
     4981 ttatcatagc caatacagga atcgccattt ttcaggaaat tcgatccaaa aagatcttgg
     5041 acgaactaaa tgttttaaat acagcaaaag tcaatgtcat acgggacggg atagaacaaa
     5101 caatagatat agaagacctt gtaatagatg acattgttat tttagagact ggacatcaaa
     5161 ttccagcaga tgcagaggtc gtggaaggaa aactgcaagt taatgaggct ttattgacag
     5221 gcgaagcaga tgaaataacc aaagaaatag gcgatccatt aatgtccggc agtttcattg
     5281 tttccggaaa agcttacgcc cgattagaca aagttggagc agattcgtat atttcccagc
     5341 tgacagtcaa agccaagaca atgggagaag gagaacagtc ggagatgatt gcttctctta
     5401 atcaattgat caaatggatt gggatcatca ttattcctat cggtatcctg cttttttctc
     5461 aaagttactt ttttaatgga aacacgatca aagaaagtgt cgttgcaatg gaagcagcat
     5521 tgatcggtat gatacctgaa ggactgtact tgctgacgac gattgcatta gcattaagtg
     5581 cgactaaact agcaaagcaa agagttcttt tacacaatat gaaaagcatt gagacattgg
     5641 caagggtaaa tgtcctttgt gtagataaaa caggaacgat cacagaaaat aaaatgtctg
     5701 ttcaaaaagt gatcgtctct aaaaaacaag aaataatcag tcagtcagat caattggaag
     5761 catgcattgc tgactatgca aaagcgatgg caggagacaa tgcaacaatg gaagctgtta
     5821 aagcatattt tacaaaaacc actgatcaat cctatcacca tgttattcct ttttcttctg
     5881 tccaaaaatt cagcagtgta gcgttggcag acaaggtgta tgttttagga gcgcccgaaa
     5941 tggttttgcg cgatcaattg ccggaatatg cggaagagtt tgtttctttc gctgaaaaag
     6001 gctatcgcgt gttggtcttt ggtgtgtacg atgggattct tgaagaacca gtattgacag
     6061 aagcagtcac accattaggc tatattctga ttgctaatcc tatcagaaaa gaagcaagag
     6121 ccacatttga ttacttcaaa aaacagcaag tagccatcaa agtgatttct ggagataatc
     6181 cgctcaccgt atccaatgta gctgtccaag cagggatttc acatgctgat cagtacgttg
     6241 atgcatctaa attatcagaa gaagaatatg aagcagctgt cgaaaaatat acagtttttg
     6301 gacgcgtcaa gccagaacaa aagaaaatct tcgttcaatt gataaagcgt aacaatacag
     6361 tagctatgac aggtgatggc gtgaatgata ttctagccat gaaagaagca gactgcagta
     6421 tagcgatggc ttccggaaat gaagccgcca tgcaggcctc acaagtggtc ttacttgatt
     6481 ctgacttttc acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg aacaatattg
     6541 agcgatcagc cagcttgttt ttagtgaaga atattttctc ctttttgctc tcactgtttt
     6601 cagtgatctt tgctctgacc tatcctttag aaccatcaca aatcacattg attagtttgt
     6661 ttactatcgg acttccatct ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag
     6721 ggaaattcat catgaatgtg atggaaaaag ctgtgccagg tggactaaca gatatgatcg
     6781 tagtcggtgc gttggtgatc tgcggggtga ctttgaatct aaataaaaca gatgtttcta
     6841 cagcatctac catgcttttg attgcagtag ggttcctcgt tctctataaa atctgttcac
     6901 cgctgaataa attcagaagt cagatcattc ttttctgtgc tagcggtaac tttttttcgg
     6961 tcatcttttt gcataagctg ttttcaatca ccggtatctc agctgtatca cttttgctgt
     7021 tgagcattct atttttctcg gcagattcga tttttaggca tttgaccaca ttggtagaat
     7081 atttgtttga acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat
     7141 ttctatcgat atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga
     7201 acaaccactc gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa cgcacgagca
     7261 gttgctcttt tttacatttt cggaatggta acggtgaaag tcgaacctgt tccaagatga
     7321 ctgtctacag taatcgtacc attaaggagt tgcacgtagt cttttacaat cgctaaccct
     7381 aatccggtcc cgcctgaatg tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag
     7441 atccgttcct gatcctcaat gtcgatacct ataccaaagt cttggacaga aaacgataga
     7501 tcatctgttg cctgatagcg gataataatc tcgctgtcgc tttttgaata ttggacagca
     7561 ttttcaatca gatttttgat aattggataa aggatatctg ttttagtagt aaatggttcg
     7621 ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg ataattttga
     7681 acgatttgct ggaaataagg ttctgtataa attgtctgga tctcgtaaga attctcgcca
     7741 tcttttgaca gctggatgat ttcccgaatc agtttatcta accggatcgc atctttttgc
     7801 atgatctcaa gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc
     7861 tcggtaaaac caatcagtga agtcacagga gtttttaatt cgtgagagac gttgccaacg
     7921 aaatcctttt gtagcttttc taaacgtcgc actttcgtta agtcatacgc cacccctaga
     7981 atctgtccgg taccttctga attagtaaag tagcgcaaac tcaagtctaa tgtctgccga
     8041 ccttctgtaa gggtgatctc ctgatggatc agcggatgct caggagtcac ttgatgaatc
     8101 agctgaatga gttttggctc ttgaatgact tctgtgtacc gctgttcagg ttgatagctt
     8161 accacgttta aatgtagctg catctttgga ttaagcagta gtaaacgaga atcagcatca
     8221 tcaatcagga atacgccaat catcagttcg ttcagtaacg tatataattg ctcttctgtg
     8281 gtagtacacg cccgatacat cttactcatt tgttcgctca acgcattaat cgtctgatac
     8341 aattcttccc attgatcggg tgtttgcatg attacttccg cctgattagg attttgcacc
     8401 atttttttta gtacgggcag cacggttttc aatggctggt ttttttgata gatcagataa
     8461 tagatcattc ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac ccaacggcga
     8521 aaactctcag ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa aatggcttct
     8581 aactggccac tttttttgac tggtaatgcg acatataaaa gatcctcatt caaagtagta
     8641 cttttgcgca aagcgctacc taggttccca ccattcaata cggctttgat ttcgggacgg
     8701 gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa ataagatctt tccagtagca
     8761 tccattaaag taatccgttc gttcgactga tggacgaatt cttccaaagc agttttgttt
     8821 tgcgctgctt ggaggttttc gacatctaat tgccggatca gcagttgtgc tttttttgtc
     8881 aggtagcttt cttgctgttc cacgatctgc tgttgaaaat aatgggaaat caattgccag
     8941 ccaccaaaaa acaaactaac taaaagaata agaaagaaga gacgttcata aatttttttc
     9001 catttcatcg ttttggttcc tgaaacttat agccaaatcc tctaatagtc accaagtact
     9061 ttggatgttt tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt acatctacga
     9121 ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga
     9181 tcacacggtc ttttcgtttc atgaaataaa caagtaattc aaattctttc ggtgttagtt
     9241 caatcagatg gtcgtctacg gtcacttgat aattggtcaa atcagcagtg atttgtccaa
     9301 tcgatagata ttc
//
LOCUS       DACEZN010000107         9292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEZN010000107 DACEZN010000000
VERSION     DACEZN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.107"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(133..306)
                     /locus_tag="IXH60_002023"
     CDS             complement(133..306)
                     /locus_tag="IXH60_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1990931.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IXH60_002024"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IXH60_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IXH60_002025"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IXH60_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1990932.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IXH60_002026"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IXH60_002026"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1990933.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IXH60_002027"
     CDS             5704..6489
                     /locus_tag="IXH60_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1990934.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IXH60_002028"
     CDS             6502..7353
                     /locus_tag="IXH60_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1990935.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IXH60_002029"
     CDS             complement(7477..7929)
                     /locus_tag="IXH60_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1990936.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IXH60_002030"
     CDS             complement(8394..8762)
                     /locus_tag="IXH60_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1990937.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..>9292
                     /locus_tag="IXH60_002031"
     CDS             8912..>9292
                     /locus_tag="IXH60_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1990938.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGR"
BASE COUNT         3148 a         1551 c         1918 g         2675 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta ga
//
LOCUS       DACEZN010000108         9233 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEZN010000108 DACEZN010000000
VERSION     DACEZN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.108"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..123
                     /locus_tag="IXH60_002032"
     CDS             <1..123
                     /locus_tag="IXH60_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1990939.1"
                     /translation="TFISSVSNQRNYIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            260..1198
                     /locus_tag="IXH60_002033"
     CDS             260..1198
                     /locus_tag="IXH60_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1990940.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            1213..2043
                     /locus_tag="IXH60_002034"
     CDS             1213..2043
                     /locus_tag="IXH60_002034"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1990941.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            2087..3112
                     /locus_tag="IXH60_002035"
     CDS             2087..3112
                     /locus_tag="IXH60_002035"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1990942.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            3127..4065
                     /gene="galU"
                     /locus_tag="IXH60_002036"
     CDS             3127..4065
                     /gene="galU"
                     /locus_tag="IXH60_002036"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1990943.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            4191..4631
                     /locus_tag="IXH60_002037"
     CDS             4191..4631
                     /locus_tag="IXH60_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1990944.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            4634..5236
                     /locus_tag="IXH60_002038"
     CDS             4634..5236
                     /locus_tag="IXH60_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1990945.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(5298..6401)
                     /locus_tag="IXH60_002039"
     CDS             complement(5298..6401)
                     /locus_tag="IXH60_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1990946.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            6611..7612
                     /gene="ccpA"
                     /locus_tag="IXH60_002040"
     CDS             6611..7612
                     /gene="ccpA"
                     /locus_tag="IXH60_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1990947.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            7966..8361
                     /locus_tag="IXH60_002041"
     CDS             7966..8361
                     /locus_tag="IXH60_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990948.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            8358..8612
                     /locus_tag="IXH60_002042"
     CDS             8358..8612
                     /locus_tag="IXH60_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990949.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            8655..9050
                     /locus_tag="IXH60_002043"
     CDS             8655..9050
                     /locus_tag="IXH60_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990950.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            9050..>9233
                     /locus_tag="IXH60_002044"
     CDS             9050..>9233
                     /locus_tag="IXH60_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1990951.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPI"
BASE COUNT         3188 a         1600 c         1967 g         2478 t
ORIGIN      
        1 acatttattt ccagtgtcag caatcaacgt aattatattc caagaaaatc attgaattac
       61 agaacaccaa tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt
      121 tgacaaatca tatataatta agaatgcgtt ttctattaca ttggtaatta aataaaaacg
      181 ttttttttaa ttggtagtac ttttggctag acaatgataa aatggtagag aacactctaa
      241 tagaaatgag gtagcatgaa tgtcagaagt tgtaaaagtt agccaattag taaaaaaatt
      301 atctttggaa atcgtcacag gcgacgagaa aagtttagac cgcgtcatcg tgactgggga
      361 catctcacgc ccaggactag agttgaccgg ttattttaat tattattctc ataatcgttt
      421 acagctattt ggcagtaaag aaatcagttt tgcggaacgg atgatcccag aagagcgttt
      481 gatcatcatg agaagattat gtagtgaaga tatgcccgca tttattattt caagaggatt
      541 gccggctccg gaagaaatga tccaagcagc caatgaaaat gggatagctg tgctgcgttc
      601 gcctatctca acgtctcggc tgctaggtga attatctagt tatttagatg ggcgcttagc
      661 ccctcgaaca agtgtccacg gtgtgttagt cgatgtctac ggattaggtg tactaattca
      721 aggggacagt gggataggga aaagtgaaac ggcgttggaa ctgattaagc gcggacatcg
      781 attgatcgca gacgatcgag tagatgtcta tcagcaagat gaactgacgg taatcggaga
      841 acctcctaaa atcctggaac atctgattga gattcgaggg atcgggatca tagatgtaat
      901 gaatcttttc ggtgcgagtg cggtacgagg ctctatgcaa gtccagttag ctgtttatct
      961 agaagcatgg gcgaaagata agaaatacga ccgtttgggt tctgaagaca cgaccgttga
     1021 aatcgcggaa gtgggcattc cacaaataaa aatccctgtc aaaacaggac gaaacgttgc
     1081 aatcatcatt gaagtagccg cgatgaattt ccgcgcgcgt acaatgggct ttgacgcaac
     1141 gaaaacattt gaagaaagat taagtcgctt gattgaagaa aactcaggca atgactagca
     1201 aggagtgaca atatgttagc acaaatcaat cgagttgctt ttgagcttgt aggaattcct
     1261 atctactggt atgcattgat tatcgtatca ggaatcatta ttgccatgtg gctcagcagc
     1321 agagaagctg ttcgggtagg attaaaagca gatgacgtga cagattttat gttgtgggga
     1381 ttgccgattt ccattattgg tgcccgtttg tattatgttt tattcgatct tccccaatat
     1441 atagctgatc caatccaaat attcaatatc cgttcaggag gattagcgat ttacggggga
     1501 ttgattgcag gtgcaatcac actgtacttt tttaccaaat atcattttat ttctttgtgg
     1561 acctttttag atatcgctgc accaagtgta ttactggcgc aggcaatcgg acgatgggga
     1621 aatttcatga accacgaagc gtatggtcca gacacgacac gaagtttctt agaaagtctg
     1681 catttaccgc aattcatcat tgataacatg tatatcgaag ggatctatcg acaaccaacc
     1741 tttctttatg aatcactttg gagtttgatt ggatttattc tgcttttggt tttgcggaag
     1801 aaaccgcatc ttttgaaaaa aggagaagtc cttttatgtt atctaatgtg gtatagtttc
     1861 ggacgtttct ttatcgaagg attacgcaca gacagccttt actttttagg tgtgatccgc
     1921 atttctcaat tattgtctgc tgttttactt gtcggcacat tgattctgtt tatttggcgt
     1981 agaaaaaaag gaaatttacc ggattatgac cgctcattag gaaaaaatca acaaattatt
     2041 tgatttttag agcgttaaaa atgaaaaaaa tttcaggagt ggtaaaatga attatagagt
     2101 agcggtttta ggaccaggct cttgggggac tgctttagca caagtcctcg ctgaaaacgg
     2161 acatgacgtt cggatttggg gacatcgtgc ttcacaagta gaagaaatca acacccaaca
     2221 caccaatcaa cgttatttac cagatcatca tctacccgat tccatccgag cattcaccga
     2281 tatggaagat gcagtaaaag atgcagatgc ggtactattc gttatcccga cgaaagcgat
     2341 acgttctgtt gcaaaagaac tagttgccaa attgaatacg aagccagtga tcatccacgc
     2401 aagtaaggga ttagaacagg gaacgcataa aagaatctct gaaatcttgt tagaggaaat
     2461 cccgacagaa aaaagaaaag ctgttgctgt tctttctggg ccaagccatg cggaagaagt
     2521 agcaaagcat gacattacga cgatcacagc ggcaagcaca gatgcaaaag cagcaaaata
     2581 tgtacaagag ctgttcatga accattattt tcgaatctat actaaccctg atgtggttgg
     2641 tgtagagatg ggggcagctt tgaaaaatat tattgccatt ggagctggtg ctattcacgg
     2701 cttagggttt ggcgataatg caaaagcggc aatcatgacg cgcggattag ctgagatcag
     2761 ccgtttaggt gtagcaatgg gtgccaaccc gctgaccttt attggcttaa gcggagtagg
     2821 cgatctagtc gttacttgca caagtgttca ttccagaaac tggcgcgcag gcaacttgtt
     2881 aggacaagga cagcctctag aagaggtgct agccaatatg ggaatggtcg ttgaaggtgt
     2941 gaatactact caagcagcga tggaactagc ccaaatgatg gaaatcgaaa tgcccatcac
     3001 tcaaacaatc tatgaagtgc tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat
     3061 catgttacgt gatgggaaaa aagaaaatga attctcatta acaatttttt gaaggagcga
     3121 atgtatatga aagtaaaaaa agcagttatt ccagcagcag gattaggaac aagattttta
     3181 ccagcaacaa aggcgatggc aaaggaaatg ttgccaatcg tggacaaacc aacgatccaa
     3241 ttcatcgttg aagaagcgtt gaaatcagga atcgaagaca tcttgatcgt tacaggaaaa
     3301 gctaaacgac caatcgaaga tcactttgat tcaaatatcg aattagaaac aaacctaagt
     3361 gaaaaaggaa aaatcgaatt attgaaatta gtagaagaaa cgacagatgt caacttgcat
     3421 ttcatccgtc aatctcatcc aaaggggtta ggacacgccg ttcttcaagc aagagccttt
     3481 attggaaatg aaccgtttgt cgttatgcta ggcgatgata tcatggaaga cgaagtacca
     3541 ttgacacagc aattgatcga tgactatgaa cgtacacacg cttctacgat tgccgttatg
     3601 caagttcctc acgatgaaac ttcaaaatac ggaattatca acccagggga agtactggaa
     3661 gatgggttat acaatgtcaa aaactttgtt gaaaaaccag atccaagcaa agcaccaagt
     3721 gatttagcta ttattgggcg ttacttattg actccagaaa tctttgatgt attatcagaa
     3781 caacaaccag gtgctggaaa tgagatccaa ctaactgacg caatcgatac gttgaataaa
     3841 acacaacgcg tatttgcccg taaattcaca ggtaaacgtt atgacgttgg tgacaaattt
     3901 ggttttatga aaacaagtat tgaatatggt ctgacacatc cagaagtagg ggcacctttg
     3961 cgtgaataca tcatgtcttt aggtaaaaca ttagaagcag aaggaagcaa gatcgatcat
     4021 aatccaaaag gaataacaga aatcaaagaa gaaaagaaaa aataatcatt tttttctaac
     4081 gagcttcagc tgctaattga ataaaaagta gttgaagctc gtttttattt gtttaaaaaa
     4141 cacgaaaatg ctatccttat agaaaagaga gacaagaagg gagtttgatc atgtcaggag
     4201 gagaaattgc aggattgatc gcagcaatcg cctttgcgat acttgtcgtt ttcatcattc
     4261 gcgtattgtt gcaagtttcc aaaactttag aaaaagtaga ccagactgtc gcagaagcaa
     4321 atacgacaat tgagatcgtc acaaaagacg tagatctttt atctagacaa gtagaaggct
     4381 tgctggtcaa aagtaatgag ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc
     4441 cattgttcac agcagttgct gatttaagtg aaagtgtatc ggaacttaat cattctagta
     4501 aaaacatcgt gacgaaagtc ggcggaatcg gaaaaacgac agctaaagcg actgttgccg
     4561 gaaaagtcgg tggaacagcg atgaaatttt ttaaaaacaa aaaacatact gaaacgacag
     4621 gaggaaaata agaatggcta aaaaaggtgg atttttatta ggtgcagtag ttggaggtac
     4681 agccgcagca atcgccgcat tattgttagc acccaaatct ggtaaagaac ttcgggatga
     4741 attggctgct caagcagatg atttgatgga catggcttca gattacacag atatggcaaa
     4801 agaaaaatca gctgagttaa caggcattgc aaaagaaaaa gcgcaagatc tttctcaaca
     4861 agcaggggat ctagcaggta atgttaagca aaaggtaagt gaatcagcag aggaagcagg
     4921 aagtgaatca gatgatatct tgttaaacct caaagaacaa tcagctgatt tatctaatcg
     4981 tttcaaaaaa acagcagaag atctgaaaaa accagcagac gaattaggta gtattgctaa
     5041 agatacttca gaagatatca tgatcgatgt aaaagatgct gcagataaag caggagagac
     5101 ggtcaatgaa ggaatgtcag aagcaaaacc agttgcagat gacacaaaag aaatgttgaa
     5161 aatggcaaaa gaagatgcaa aagaagctaa aaaagaaatg aaagatacaa tgacagattc
     5221 gactaccaat cactaagaat actcatttct agaataaaaa ggtgagagcg gaccagctcc
     5281 cacctttttt attttcatta ctctaaaaca agtaattctt ttggtgtagt cgtgaatggc
     5341 acacaaccat cacttgtcac atacacacaa tcttcgatac gtacccctac tttttcagga
     5401 atatagattc ctggttcaag agagaagcac atcccttctt cgatcactaa gtcgtttcct
     5461 tctacaagag aagggaattc gtggacggtt gtcccaattc cgtgacctaa acggtgattg
     5521 aaatattcgc cgtagccata gctgttgatt acgtttcgtg cgatttgatc aagttcacct
     5581 gctgtcacac ctgggcgaac tgcctcttgt gcggcaagct gtgcttcaag agtgatgttg
     5641 tagatttttt cttggaattc tgttggtttt tgataagcaa ctgtacgagt agcatcactg
     5701 caatagccat tccaaacaac gccaagatcg aataacacta gttcatttgg tgaaaccttc
     5761 gtgcttcctg gtgtaccatg agggcttgca gcatttgctc ctgcaaggac taaggtgtcg
     5821 aaagacattt gtgaaacacc ttgttttttt aattggtatt cgatttctgc gataattgct
     5881 tgttcagtca cgccttcttt tactgttttg aagccaattt caaaggcaac atcggcccat
     5941 ttaccagctt cgatcagttt gtctttttct tctaccgtct tgatcagttg cagacgctgg
     6001 ataatagaag ttagatcagt gtaagaatct gcacctgtca agatatcaga gatagcatcg
     6061 aagcgatcga cagtcagagc attcttttca atccctacat taccaggttg cccatagcgt
     6121 gcgcgaagct cttttccgat gatctcccaa gggttttcgc tgtctaagta gccacgtaca
     6181 tcgtacgccc agctgctgtt ttgagcatct tcaacttcca gagcaggagt gaaaaggaaa
     6241 ggatcttgtt caagagggat aaaaagagct agtacacgct cgtgaggatc actttcatat
     6301 ccggaaaagt aagcgatgtg gccagggtcg ctgatataag ccaattctac ttggtgttgt
     6361 gccatccatt gacgtagttc attgattttt acgttattca tttggcaacc tactttctaa
     6421 attaattttg tattcaataa ttgtaccact gattgattaa aagaccaacg ttcttaaaac
     6481 tgaaaaaagt aaaaattttc atgaaaacag agataaaacg gttgcaattg ttgagattat
     6541 ttgatatcct attttagaaa tgtaagacgt attttcagaa aacatataaa atatgaatag
     6601 gagaacacaa atggaaaagc aaacaatcac tatctatgat gtagcgagag aggcaaatgt
     6661 ttcgatggct actgtttcac gtgtcgtaaa tggcaatcca aacgtcaaac cagcaacaag
     6721 aaaaaaagtc ttggaagtta tcgatcgctt ggattatcgt ccaaatgcag tcgcacgtgg
     6781 cttggcaagt aagaaaacaa caactgttgg ggtaatcatt cctgatgtca gcaatatgtt
     6841 ctttgcctct ttagcacgtg gtatcgacga tgtagcaaca atgtacaaat acaatatcat
     6901 cttagcaaac tcagatggca acgatcaaaa agaagtcaat gtattgaata atttattggc
     6961 taagcaagtg gacggcgtga tctttatggg tcaccatatc acagacgaaa tccgtggaga
     7021 attttcacgt tcaaaaacac ctgttgtact agctggttcc attgatccag atgaacaagt
     7081 aggtagcgta aacattgatt atgtatcagc aacaaaagat gcagtaaata aattagcaaa
     7141 aagcggcaat aaaaaaattg cttttgtcag cggtgcgctg attgatccta tcaatggtca
     7201 aaaccgtcta aaaggctata aagaagcatt aagcgaaaac aatctaccat atagcgaagg
     7261 attaatcttt gaggcacaat acaactttaa agatggtctg tctctagttg accggatcca
     7321 taatagcggt gcaacagctg tctatgtttc agacgatgaa ttagccattg gtatcttgga
     7381 tggtttatta gaccgtggcg taaaagtacc agaagaattc gaaatcatca caagcaacaa
     7441 ttctttactg acagaagtag ctcgtcctcg tttgacaagt atcacacaac cattatacga
     7501 tttaggagcg gtatccatgc gcctattgac gaaaatgatg aataaagaag aagtcgaaga
     7561 aaaaacgatt atcttgcctt atggaatcga agaaaaagga tcaactaaat aataaaagat
     7621 caatcattta gaggttgttc caaaagtggc caacttctgg aacaacgttt attcgagaaa
     7681 aggggttatg acaaaatttt gtcataaccc cttttttttg tgactttaaa aaaagagaaa
     7741 atggcggaaa caaaccttac gtccatcgta tacgacacaa tctgtgcgta gatgctacat
     7801 tttgttcact actatcaaaa ttactttaaa atgaaaacgt gataaaaaag aaagtagaaa
     7861 agtagaaaag taactctaaa aaatccaaaa aaatccaggc tcgattgcgt acttatctta
     7921 aaggagaaac aatccatcat acataacccg aaaggagact tctatttgcc taagaaaaga
     7981 cgaaagacca tagtcatcat tttgaccatt cttgctatag gaatcggagg aaaattttac
     8041 atggacaaaa gagaagcaca aagacagaaa gatttactcg cagtagaaaa acaatccgtc
     8101 aaagttctga aaaatacctt tgcagatatc agggaagtga aggtagaaga atttaaaaaa
     8161 aaccctgtta ctggatgtta tggtgcactt gtgacaatga caaataatga agggaaatct
     8221 gtttattttg attatttttt ttcaaaacaa atgaatgaaa ttagcagtta tggtgtagaa
     8281 aatgaagaaa tacaaaacga aggggttact actcctaaaa taagagttat ttattctaat
     8341 ggagaggcgg aggaagtgtg acagtaaaag acgaagagta tagatatata agtgaacatg
     8401 tttattggct agatccaaaa catcctcaat atagtaaaag ttacaaagaa ggcgccatta
     8461 aaaactttgg agaaacaaaa ttccaaatcc taaaaatcaa agactcattg gatggcatgc
     8521 aggcgatggc tgcagcgcca atcgtccata gcaaattgga gaaaaatttc aaaaataaaa
     8581 aaattcctac aaactttcgc gtacttaaat aatgaaggta tcaatactta cataaaaaga
     8641 aaggaggcac gttcttgtct cggaaaataa gaaaactcag cttagccatt ttattaatcg
     8701 tcatattagg aatcggaggg aaagtctata tggacaaaag agaagcacaa agacagaaag
     8761 atttactcgc agtagaaaaa cagtcagtca aagttttgaa aaatactttt gcggatatca
     8821 aggaagtgaa gattgaaaaa tctgctcgaa atgaaatgac gggagcatat cgtatgtttg
     8881 tgacaatgat aaataatcac gatgagtctg tttattttac gtttggattt tggaaagaac
     8941 gaaatgaatt aggagattat ggcgtagaaa ataaaagtat acaaaaagag gggattacag
     9001 taaataaaat cagagtaatt ttttctaata ataaggagga atatatttaa tgatggcaga
     9061 caaacaatac agtgttatta gtgaggctgt ttactggcta gatcctaaac acagagacta
     9121 tgatcatact tataaagaaa attctattaa aaaaattgat agttataatt ttaaaatcct
     9181 aaaaatcaaa gactcattgg acggcatgca ggcgatgggt gtagcgccaa tca
//
LOCUS       DACEZN010000109         8962 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEZN010000109 DACEZN010000000
VERSION     DACEZN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.109"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            196..1044
                     /locus_tag="IXH60_002045"
     CDS             196..1044
                     /locus_tag="IXH60_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1990952.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            1275..1754
                     /locus_tag="IXH60_002046"
     CDS             1275..1754
                     /locus_tag="IXH60_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990953.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            1787..4015
                     /locus_tag="IXH60_002047"
     CDS             1787..4015
                     /locus_tag="IXH60_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1990954.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            4144..5787
                     /locus_tag="IXH60_002048"
     CDS             4144..5787
                     /locus_tag="IXH60_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1990955.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(5860..6405)
                     /locus_tag="IXH60_002049"
     CDS             complement(5860..6405)
                     /locus_tag="IXH60_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1990956.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(6428..6913)
                     /locus_tag="IXH60_002050"
     CDS             complement(6428..6913)
                     /locus_tag="IXH60_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1990957.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(6903..7715)
                     /locus_tag="IXH60_002051"
     CDS             complement(6903..7715)
                     /locus_tag="IXH60_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1990958.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(7854..>8962)
                     /locus_tag="IXH60_002052"
     CDS             complement(7854..>8962)
                     /locus_tag="IXH60_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1990959.1"
                     /translation="ILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAHS
                     MGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKVV
                     HMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTSM
                     AQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLPE
                     NTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLIE
                     TINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELPR
                     LNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         3040 a         1682 c         1768 g         2472 t
ORIGIN      
        1 ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct
       61 gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt
      121 agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac
      181 gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa
      241 cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa
      301 atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt
      361 aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa
      421 ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc
      481 tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat
      541 gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat
      601 gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc
      661 tacgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat
      721 ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg
      781 aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa
      841 attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca
      901 aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca
      961 gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc
     1021 ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca
     1081 acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt
     1141 ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc
     1201 gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat
     1261 gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag
     1321 aagactatga taaagttgat ggaagaaatg cattaaatac ggatgcaaaa gggctttcac
     1381 tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac
     1441 atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt
     1501 tagcgatctt agctgcacaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat
     1561 tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg
     1621 tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac
     1681 tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg
     1741 agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc
     1801 actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa
     1861 aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca
     1921 agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac
     1981 acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt
     2041 tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa
     2101 ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact
     2161 gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga
     2221 gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc
     2281 tgaaatcgtt cgagcgattg aaaaaggata tttattagag attcaagagc cgactgtcat
     2341 ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa
     2401 tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg
     2461 aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga
     2521 aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat
     2581 ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc
     2641 aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac
     2701 ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc
     2761 cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact
     2821 ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa
     2881 aggaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga
     2941 aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga
     3001 gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag
     3061 tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat
     3121 gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc
     3181 gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc
     3241 tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga
     3301 atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa
     3361 aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa
     3421 ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga
     3481 tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc
     3541 cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga
     3601 ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca
     3661 atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc
     3721 acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa
     3781 tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga
     3841 tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat
     3901 tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac
     3961 tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa
     4021 taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa
     4081 caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac
     4141 gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct
     4201 agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt
     4261 aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca
     4321 tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg
     4381 atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg
     4441 gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct
     4501 aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg
     4561 aataattgga catcgatttt cggcggttct gcttgggagt attctcatga aactggacag
     4621 tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg
     4681 aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt
     4741 ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct
     4801 ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt
     4861 tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga
     4921 ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat
     4981 atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaact tgcattaagc
     5041 gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat
     5101 gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca
     5161 aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag
     5221 gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg
     5281 aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc
     5341 attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat
     5401 gctggttttt ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat
     5461 gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc
     5521 attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat
     5581 ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac
     5641 ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta
     5701 ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa
     5761 gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt
     5821 gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc
     5881 accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa
     5941 acgaggtctg acaggtactt ggacatgttt gatatgcata ccgattgatg tgtcaccgat
     6001 atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa
     6061 agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa
     6121 attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc
     6181 ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca
     6241 ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct
     6301 gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac
     6361 tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct
     6421 ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg
     6481 caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg
     6541 gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta
     6601 ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc
     6661 cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat
     6721 cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag
     6781 cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga
     6841 tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt
     6901 gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta
     6961 tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct
     7021 actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca
     7081 caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga
     7141 tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc
     7201 acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac
     7261 gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc
     7321 gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct
     7381 tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt
     7441 atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca
     7501 tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata
     7561 gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt
     7621 ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat
     7681 ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt
     7741 ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt
     7801 aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac
     7861 ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc
     7921 gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc
     7981 tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga
     8041 gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa
     8101 aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc
     8161 aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca
     8221 taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac
     8281 agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc
     8341 cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa
     8401 ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga
     8461 agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt
     8521 atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta
     8581 gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga
     8641 gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat
     8701 cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga
     8761 aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc
     8821 catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta
     8881 tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt
     8941 tgttccatta aataaagaat ag
//
LOCUS       DACEZN010000110         8952 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEZN010000110 DACEZN010000000
VERSION     DACEZN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.110"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            153..524
                     /locus_tag="IXH60_002053"
     CDS             153..524
                     /locus_tag="IXH60_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990960.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            517..912
                     /locus_tag="IXH60_002054"
     CDS             517..912
                     /locus_tag="IXH60_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1990961.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            914..1291
                     /locus_tag="IXH60_002055"
     CDS             914..1291
                     /locus_tag="IXH60_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990962.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            1292..1900
                     /locus_tag="IXH60_002056"
     CDS             1292..1900
                     /locus_tag="IXH60_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1990963.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            1900..2241
                     /locus_tag="IXH60_002057"
     CDS             1900..2241
                     /locus_tag="IXH60_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1990964.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            2483..5272
                     /locus_tag="IXH60_002058"
     CDS             2483..5272
                     /locus_tag="IXH60_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1990965.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            5262..6002
                     /locus_tag="IXH60_002059"
     CDS             5262..6002
                     /locus_tag="IXH60_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1990966.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            5999..8710
                     /locus_tag="IXH60_002060"
     CDS             5999..8710
                     /locus_tag="IXH60_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1990967.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSIIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            8723..>8952
                     /locus_tag="IXH60_002061"
     CDS             8723..>8952
                     /locus_tag="IXH60_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1990968.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKI"
BASE COUNT         3204 a         1194 c         1808 g         2746 t
ORIGIN      
        1 aaaaatgaat cagaaaaaac agtatcttca gttagacgtg gcgatacaac gattagttat
       61 gcttcaacaa caaatgatgt tagtgagtta cttctaggct atggtgattt gatacgaaga
      121 gttattggtt gtggaggatt ggagtttttt taatgaatga agcagatatt ttagaaacta
      181 cttatgaaga tagttgtatt attgaaagac tgacggacat tgaagattct aatacaaata
      241 ttactattca agattacaaa aaagtatatg ataatcctat ttcttgcgct ctttcacaag
      301 gtcaaatcga tggactagca gtcatagaag atggagagat ggtaaatgtt tcaactgaca
      361 catataaatt atttgttcat cctaagatta aactaaaaaa aggagatcga ataacaataa
      421 ctcaaaaggc cagtggctta attttttctc tatttgctac taagcctttt tactatccta
      481 gtcattgtga agtgaatttg ataggaagtg agaaaaatgg gtaatctcaa atatgaatca
      541 aatgcagaaa agatcattga gaattttaaa aatatgactg taattgctca aaaagaagga
      601 atatccttcg ttaacgattc aatgaataaa gtcgttagtt tagctaaacc attaactcct
      661 gtaaaatcag gtaatttaag gcgaggatat cgtgtagtaa aagctagaaa actatcaagc
      721 ggtcgaattg ttggagcagt tataaacaat gaacattatt ttaaatatgt agaagaaggt
      781 cataggacta aaaatggtgg ctttgtcaaa gggaaattca tgttaactcg tgcaacgaat
      841 cttgcaaata tgtcttatat tcctcgaaga tttaaacaaa tggcaataaa aatcgttaag
      901 aaaggaaagt aacatgaaag atgaaatcat tgctgcaatc agtagcaaat taaaagaaat
      961 ctatcctgat gggacaatat atcttgattc agttatgcag tcaactaaag atttttattt
     1021 cgttttatca gtaatggaat ctggaactga aaatgtagga attgatattc aaaatgtttc
     1081 tttcttaatt gatattgcat tgattgataa taaacctaat agaaatttaa ttaatgaatt
     1141 agtctcacgt tgtgggactt tttttaatac gattacaatc gatgagcaaa tattatttcc
     1201 aaaagcctat ttacctgatg aagcagatgg tgttcaacat attcgtttta cattagaatt
     1261 tccacaatat attgaatgga gtgaaagata aatgggagaa aaaagaagta aaaccggaat
     1321 tatttctgtc gaaaaaccta cttggtttcc tttggaagat gagacaggga atttcccggt
     1381 atatggtact gcgttgccaa tgggaacagc agtaagtatc aaaccaacag ctaattatga
     1441 aacaacacaa gattatgggg attcagtggt tcaagatcaa tttacagctt ttggtggtgc
     1501 agaagttgaa ctggaagcaa atggatatac tcataaagtt ttatctacaa ttactggagg
     1561 gaaaattgtt aaaggtggtg ccttgcgttc tggagaagat attgcgcaag atggtgcgtt
     1621 tgcatataga cgtaaaaaat cgaatgggaa ataccgttat acggtctttt ataaaggaca
     1681 gtttgctcta gattcagatg aaacgtcaac gattgaagga agtaaagtaa gttttactca
     1741 tccaacttgg aagggttcat ttgtagatgt accaggactt ggatatatgt attcagttga
     1801 tgaagatgat gaaggcgtgg ataaagcaat gatcggaaat tggtttacaa aagtagcaat
     1861 tccaattgaa gaagcagaac tttcaggagg tacagaataa tgtcaaaata tcaaacaacg
     1921 attaaattaa cgaaaaaaga tgaagaaggt aaatatgagc aagtacaatt taaatctgct
     1981 gaatttttac caggaacagt tgtagaagat gctgcaggag ttatggaaga aatgcaaaca
     2041 gcgactgata aacaatcagt taaaaaagct ttaagtcgtg cttattcatt tattgcagat
     2101 actttatttg agggacaatt tactggtgaa gattattgca aaggaattga tgctcgtgag
     2161 attgcctcat tgacaggaaa attattgaag tctgttactg caggttttga tgaaacttat
     2221 acagagacga aaaaaaagta agtgaggcgc tcaagtcacc ttcatttaag tattcgatta
     2281 cttaccgaga attagatata aagacgcaat tactcgaagc aggttggacg ttaccagaaa
     2341 ttgaacgtac tgacttggat gagttaatgc gtctttttgc ttttagagat gcggtaaaag
     2401 agcatgaaga tgttgagtac tatgataatt tcactcaatt ttaggaggtg atactttgaa
     2461 taacgatgac ctaattctga agatgatact agatgaatct ggttttactg ccggtatgaa
     2521 taatgctgtc aaaaagttga actcttttga tgaaacaatc gaaagaaaaa gtagaaatag
     2581 tggcagttca ttaggtagta tttggaaaat atttgctgga agttttctag ctagcggagt
     2641 aactagaatc gtaggagctg gctttgatct aatcaagggt tccataagtg gagcggttga
     2701 tcgagtagat acgatgaata acgctctacg aaatttccaa aatatgggat ttagtaattc
     2761 agaaattatg aagaatatcg gaaagaatgg gcttttatct caaggtattc aaggacttcc
     2821 tactgctttg aatgatgcga taagtcatgt tcaacttctt gcttcttcta caggtgattt
     2881 aactcgttca actcaaatat ttaaagcttt aaatgatgga attcttggtt tcggtggttc
     2941 aactgatcaa gttaatgaag ctgttattca attatctcaa agtttctcaa acggaaaagt
     3001 agacgcacaa acttggaact caatgatcaa cgctcaactt ggacctactc tttctgctat
     3061 tgctaaaaag atgggaatta caatgggaga tctgaaagaa ggtttatctc aaggtaagat
     3121 ttctgttgaa gaattccaaa atcaattaat agaaatggat accaaaggtg gcggaggact
     3181 taaatcatta agtcaaatcg ctaaagattc tactaaggga attaaaacct ctatacaaaa
     3241 tgcaaaaaca gctgttacac gtggtgttgg tgaggtaata gaaggattaa ataaagcatt
     3301 agtagattca gatttaggtg gatttaaggg aattattgat aaagtgggca gttcaatgga
     3361 atcgttctta aaagtaattg ctgcaaatat tcctacagca gtatcatttt taagtaattt
     3421 atttaacgaa attcaaaaat ttggttctgc tttgaaattc atgatgccct ttcttgttcc
     3481 tgcagctacc gcttttggag cgttcatgtt tcaacttaaa ggtataccag caataataaa
     3541 aagcttcaat aattttaaga atgccataat tggcgttgga aattcattaa agattatggg
     3601 ggcaatagct gctgctaatt catttgtttt aattattggt gcagtcgttg gtgcaatagc
     3661 tgttttcggt tactttatgg caaccaatga agagtttaga aacaaggtta tatctatttg
     3721 gaatgatgtg aaagattccg taattggtgt attaaaaaat ataaaggact ggggaattga
     3781 tacttggaat tctgctaaag aaatggcatc aaatgctgtt gagagtgtca aggatgcttg
     3841 gtctggaata aaggaatggt tttcaaatac ttggcaaggc attaaaaatg gagcaacagg
     3901 tttatttgat aaaacagtag aaacatctat gaatgcggtt gatagtgtaa aaaacgcatg
     3961 gtcaaataca aagcaatggt tttctgatat ttggcagagc ataaaagatt cagcaactga
     4021 aaaatggaat gaaattaaag gttctatcat ggaagttgct ggtccattga ttacaggttt
     4081 aaagaacgca ttcttgcatg tcacttttta tttagagacc ttatggaata atctagttga
     4141 gattggtaaa aatgtttttg agattttaaa aaatgttata cttacgccag ttttgtttat
     4201 tacctccatg atttctggtg gatgggaaga aacaaaaaac aacatgattg gtgtatggaa
     4261 taatattaaa gaaagtgctc taaatatttg ggagtctata aaaaatattt ttgttagtta
     4321 cattacaaat atttattttg ctgcgcttaa tatttggaca gggttcaaac ttactttgat
     4381 aaacatttgg aatgaagtag taagtcaagc taaatcaatt tggattaatg taaaatactt
     4441 ttttattaat ctttggattg acattaagta ttttgctatt caaaaatgga ttgaattaaa
     4501 attcgggatt attcaaactt ggattgattt aaaatataat gctattacta cttggaacaa
     4561 tattaaacag ttcttcaaag atacttggca gaacattaaa gatatagcat acaatacatg
     4621 gatttctata aagaattcca tgatcaatac ttggaataac atcaaggaat ctttctggaa
     4681 tattgttact ggaattgtta actccgctga aaatgcatgg actaatctaa aaaatggtgt
     4741 ttcaaaagca attaatcggg tgaaagaaat ctttgattca ttaagggaaa tcaatttatt
     4801 cgaaatcggt aagaatatca ttgatggact tatcaatggt gtagtagaaa aatggaatgc
     4861 attgaaaaag actattaaag gaatagctgg aagtatcaaa gattctatta agggtgcatt
     4921 aggtattcat tctccatcta gatggatgag ggatatggta ggtaaaaata ttgttcaagg
     4981 tattatcgtt ggtattgata aagaaaaaag taaattggat cagacaatga ctgatttagt
     5041 gaaaactccg tcggttcaac cagttattac agggtctaat agtcaaccag tggtacaagc
     5101 taaacaaaat acatcttcaa atgcagtgaa tgaaattcac ttgcatttaa atgtttatgg
     5161 agatctacct gattcgatga ttaaacaaat cgccaagaag atgaaaacag aattgacgag
     5221 acaaatgaaa cgagatgctg atgcagtagg agggacatta tatgcaactt aaaagaggac
     5281 aattttttat caatcaacat tattcttctg agtttaacgt atatattcaa aataggcctg
     5341 cctctgtttc agctagtcgt gtaattgaat tgagagaacg tgaaggcaat gactcaatta
     5401 ttatcgataa agcctattac aaaaacgtta ctagaaaaat tgaatgttat tacaaagcac
     5461 catcgattga tttagtgcag gaatgggaag atcgaattac tgaatggtta gatatgaact
     5521 cttacagtga ttttattctt tattacgatc aacaatatat ctatcaagct atagtaaccg
     5581 aagcgccaga atttaaagga acaagaaaaa cagggaacat agttccattt gaatttacag
     5641 ttagtcttcg accattcaaa gaaaattata gtggtcgttt tgctattcaa cagataaaag
     5701 ctttcgagtt atttaatcca gagaagtatg cttcaaaacc gcttattaaa ttgagtggtt
     5761 ctggagatgc ttctttttat attaataatg ataaatatga tttgaaacta ttagatagag
     5821 aattatatat agattctaaa ctagaagagt cttatcgaaa gctagatgac aatttagaac
     5881 atcaagatca tgtcacttta tttttagatt ttccatttct ataccctgga aaaaatgaaa
     5941 tcaaatggac gaataacata cattcgtttg agataatacc aaggtggtgg agaaaagtat
     6001 gataccaaga atatatagtc ctaccgagac agatttttct acgaatggtt tagggatttt
     6061 gaaggacacc acaaaatgtg agatatatga agtagcaaat ggaaaatatg aattagagtt
     6121 ggagtatcca ttaggtactc gatttgatga atattttgaa aatgactatc aaataaaagc
     6181 aaagtcaaat gatcaagaag agtatcatat cttttttatt gatgataaag atattgatac
     6241 ctttttagat accgtaacta tttatgccca gagtcgtaca aatcggcttg gaagacgagc
     6301 ggtcactttt gctggagtcg actctaaaac tggtcgagaa gcaatggcaa ttattgaaaa
     6361 caatatggat aaaaaatcag atatacgctt atattctgat attacaactg tatcaagtac
     6421 aacttttgaa gcaagaaatg ttttaaattg tattgctggt gaacaaggat ctttacttca
     6481 gtattggggc ggagaaatta aacgtgagcc ttttaagcta tctttattaa agcgaagagg
     6541 tcgtgataat attggcacaa tccgatatgg aaaagattta tctgggttaa aagttaagtt
     6601 agattggaca ggcgtgaaaa caagaattat tccgtatgct gatcctcaaa gtgatgcagg
     6661 tacaactagc cgaatttatg gctcgccagt agatagcgaa tatattaata attatcctga
     6721 tgtatataca gaacacgttc aattcacaga agaacaggga gttaaagacg ttaatagctt
     6781 aaataaaata gctaagaatt atttcaaaac aattaatcca ggctgtgata aaccgaagat
     6841 ttctattacc gttgagttcg ataaattaac ggacaccgaa gaaggaaaag aattcgctaa
     6901 gataagaaat tatgggttat ttgatacgtt taaaatatat catcgaaaat ataaactata
     6961 ttttgaatcg aaagttagcg gtgttcaata tgattcatta tctgaaaagg ttttgaaatt
     7021 ggaagctggg gatgctcaag ttgcttttta tcaacaacaa gctgttacta ttcaagataa
     7081 attaaaagat tatgctacga ataattatat gagtagtttt aatgattatg tgtcctctat
     7141 tattgcaggt caaggaaatg caggcggtta tgtagtttta tggccaaaag aaaaaccttc
     7201 aaatattttt ataatggata gccctgattt aaacaaggca aaagaagtat taagaatgaa
     7261 caaaaatgga attgcctttt ctaaaaatgg ttggaatggt ccttttaatt ccgcttggac
     7321 attggatagt atattcaatg ctaattttat tcaaacagga ttaataaagg cagatatctt
     7381 tcaaaactct tttaataaaa ctggagatgt attgaaatta gttaatggac tacttcaaat
     7441 ttggaataac aaaaagaaaa taatggagtt aaccaaaaaa ggaatggagt tttggaattc
     7501 taatagttca attggaacga ttggaacaac tgattctgct ggtaatcctt ttcctggagc
     7561 ttctactccc acacctattg aagataattc tttagttatt cgtacaaatg gagacggcaa
     7621 atatattttg atttctccta aagttggtaa aggattagtt ttattaggaa atggtaaagc
     7681 aatttatttt ggagacttag atgtacaagg caaactcaca gttaacggaa aagaaatcac
     7741 agggaacaac agtggaggaa gtgatcctgg aactatccct cctcaattga cgacggaagc
     7801 tgaaaaaaga gcatggaaaa tttggacaat gctgaaagct cgtggctatt ctgaatatgc
     7861 agctgcaggt attctaggaa atattcaggg ggaggttgga gcaagtatga accctgatac
     7921 agaacaactt ggtggtccag cttatgggat tgttcaatgg gatggttctg cttatccgtt
     7981 agtcggatca ccaacttgga acggacgaga gtatgttcag cgtttgatga acaccgcagg
     8041 gattcaagaa gattatcgaa gtattgaagc ccaagtaaaa ttattagatt ggtgtatgtt
     8101 caacggtcaa tggctcggaa aagtaaatcc aaccacagta tcaggattta aatcgatcaa
     8161 tgatgccaaa agtgcagcgt atgcttttga aatgaacttc gaacgcccag cttctgcaca
     8221 tccagaacgc caaaattatg cccaatcttg gtataacaaa ttacatggat taactagtcc
     8281 agaacctgga gggaatttca tttgtccaat tcaaaaacca gtgacagtta cttcagaatg
     8341 tggatggaga actagtccaa taaatggcgg ccaagaattt cataatggaa ttgatcttgt
     8401 aaatggaaat cctaatacac ctgtttttgc agcattagat ggggaagttg ttcaggctgg
     8461 tgctaattat tatgactggt atggtaatta cgtggttatt aaacataata atgggaagtg
     8521 gacaggttat gctcatttgt ctcgtattga tgtttctgtt ggacaaaaag tccagaaagg
     8581 tgctcaaata ggcttgatgg gaacaactgg tccatctaca ggagaacatc tacattttca
     8641 aattatgaaa aattattggc cacagccagt tgttgatttt gagaatccaa gaaattatat
     8701 ccaattttaa ggtggtgatt ctatgagtaa atggaatgtc gttttaagta caacagaacc
     8761 atataattat gtggggatga ttcaagttcg acaaggcaat aagaatacag aggttatgga
     8821 agcgactata gttgaaaatg gtcttcccta cgatttatca gaatgtaagg tatattttga
     8881 atcagttgta ggtgggaaat atccagtcca attagaaaca aaaattgtgg atgctaaaaa
     8941 agggaaaatt aa
//
LOCUS       DACEZN010000111         8885 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEZN010000111 DACEZN010000000
VERSION     DACEZN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.111"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            170..1471
                     /locus_tag="IXH60_002062"
     CDS             170..1471
                     /locus_tag="IXH60_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1990969.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            1474..2325
                     /locus_tag="IXH60_002063"
     CDS             1474..2325
                     /locus_tag="IXH60_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1990970.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            2338..3153
                     /locus_tag="IXH60_002064"
     CDS             2338..3153
                     /locus_tag="IXH60_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1990971.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            3304..4470
                     /locus_tag="IXH60_002065"
     CDS             3304..4470
                     /locus_tag="IXH60_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1990972.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            4700..5347
                     /locus_tag="IXH60_002066"
     CDS             4700..5347
                     /locus_tag="IXH60_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1990973.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            5431..6375
                     /locus_tag="IXH60_002067"
     CDS             5431..6375
                     /locus_tag="IXH60_002067"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1990974.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            6510..7961
                     /locus_tag="IXH60_002068"
     CDS             6510..7961
                     /locus_tag="IXH60_002068"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1990975.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            7982..8818
                     /locus_tag="IXH60_002069"
     CDS             7982..8818
                     /locus_tag="IXH60_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1990976.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            8818..>8885
                     /locus_tag="IXH60_002070"
     CDS             8818..>8885
                     /locus_tag="IXH60_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1990977.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLV"
BASE COUNT         2840 a         1583 c         1790 g         2672 t
ORIGIN      
        1 aagccataga acaaaaatac tgattaaaat gataaccgtc catttttata aaaaatggaa
       61 caggtgcttc agataaaagt ggcaagagcg agatggaaaa ttatcttgct cttgttgcca
      121 ttatcttaaa agcagtttgc ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga
      181 taaaacaagc aatatcaacg aaaaaaaaat cacgctttgt tcacttattc cagggatggg
      241 gcagtttgta aatggccaag cttttaaagg tatcgttttt ttcgccatat tcgtcctatt
      301 cattggtcag atgcttctgg gcggatttca tgcattggta ggattggtta ctttgggaag
      361 tataccaatg gaagatcatt cattatttat tttgattgaa ggcactttgc aattcatcat
      421 tacaatcatt ttcttgattt tttacggttt gaatctttta gacgcttatc gagtagcgaa
      481 gatgcgtcaa gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac ataatatagg
      541 tgatgaaggt tttccttatc tattgacact tcccgcttat cttctcatga tttttacgat
      601 cattttccca gtcctagtta cattgttcac tgcatttact aattatgact tcaatcatat
      661 tccaccagcc agtttaattg attgggtagg ggctaaaaac ttttttagta tcttcttttt
      721 aagctcatat cgtaatactt tttttgcagt cttttcgtgg accgtgattt ggactttatg
      781 tgcaacaact ttgcaaatca cccttggtat tctcacagcg gttgttgctc accagaaatt
      841 tattaaattc aaacgtattt tcggtatcat tttcttatta ccatgggcgg tcccagcttt
      901 tattactatc atgagttttt cgaatatatt caacgatagc gtaggtgcca tcaatactca
      961 agtgattcct ttcataaacc agcttccttt tgtagatatc agtgcaatct cttggaaaac
     1021 ggacccgaac tggacgaaag tagcaattat cttagtgcag ggctggttag gatttcccta
     1081 tatctatgtg atggttacag gaatcttaca atcgatttca gaagacttat atgaagcagc
     1141 aaaaatcgac ggagcaaatg catggcagcg cttttcaaac atcacgcttc ctgctgtctt
     1201 cctagtggca gcgccaacat ttattacaca gtatacgggg aatttcaata atttctccat
     1261 gatctatctg tttaacaatg gcggtcccgg aagcgtaggc ggaggagctg gttctactga
     1321 tattcttatt tcctggatct ataaacttac gactggaact tctccgcagt attctatggc
     1381 ttcagcaatc acgttgatta tctccgtcat cgttatctgt gtgtcattgc tggtatttaa
     1441 aaagacaaat gcgttcaata tgggggatta acaatgaaaa aatttcattc gagtgctcga
     1501 ttccaaaaac gactttctca cttttttact tacttttttc ttttaatact gtcagtcatt
     1561 atcatctatc cgcttttgat cacagcaagt actgccttca agtcaggaaa tattgcagct
     1621 tttagtttag atctttcaag tgactggact ttaggaaact ttactcggct attcagtgaa
     1681 acactttacg ggacttggta taaaaataca ttgatcattt ctgtcctaac aatgatcgta
     1741 caagtgactg tagtaacttt ggcgggctat acttatagtc gctatcgttt tatcggccgc
     1801 aaatcaagtt tgatgttctt tttagtgatt cagatggtac cgactatggc agcattgact
     1861 gccttttatg tcatggcttt cttattagaa gcgctcgata agtactggtt cttgacaatg
     1921 atctatatag gcggtggaat tccaatgaat acttggctga tgaaaggata ctttgacact
     1981 gtaccaatag atttagatga atctgctaaa cttgatgggg cagggcattt caggattttt
     2041 ttccagattg tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt
     2101 atgggacctt ttggggattt catgttagct aagttcctgc ttcgaacacc tgaaaatcag
     2161 actgttgctg tcggcttaca aacatttatt tctgatgtga caaaccaaag agtctctctt
     2221 tttgctgctg gggcaatttt gattgcacta ccgatttgtc tattattctt ctttttacaa
     2281 aagaactttg ttaccggcct gacatctggc ggaacaaaag gataggagca gagtatcatg
     2341 caaacatttc cgttaaacta tttttccgca ttacgtacgc caactcaagt gtttgcggga
     2401 cgcaaactat tatcatggcc taaatttttc ttgatatttg tatttttagt ttccttgatg
     2461 gtcatgcctg tgactctttt ttatgccaat cagatccaag cgattccatt agaacaattt
     2521 ctatccgttc attcgctgat cgatgaacaa ggaacccaaa aattttctga acttgaatta
     2581 tctgaaactg ggttacaagc atcgcaacaa acgatagctg tgacccctga aattttggta
     2641 ggtgtgtctt tatctgagaa acaacaatcc gatcatggaa cttttattga ttttgagaag
     2701 gaacaatggg tgatccaaca aaaggataaa tctggtatac gccgctatac gatgaattat
     2761 agcccatctt tccaaccaga ttctgtacgt acgcccgaag atttccaacg atttttagaa
     2821 agagaatttt atgccagtaa tcgtccaaca atcattttat cttatagttt aagtcttggt
     2881 cttgtcctat ttgtcatgac gagtctcatt ttattcgggg cttccttttt cttgtggatg
     2941 acaagaaaaa gccaactttc atccattcat acattcaaag agagtgcaaa tttgatgctg
     3001 aatgttatgg gaataggttc catggcagca gcagttgtag ggtttatcca ctttgacttt
     3061 atcttgatgc taagtgtcca aacaacgata actgtcttgc tgcttttatg ggtttttgtc
     3121 aaaacaagat tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg
     3181 aatgtgaaaa aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc
     3241 ttagttgatt cttttctttt ttagtattaa cgtaataggg tagttttttt aaggaggaaa
     3301 atcatgttag agaaaacttt atacaatcaa ttattttcac gttctttctc gctccctgtc
     3361 gaagtaaatt attgggacgg tacaacgaaa caatacggag atacggacac tcctacccaa
     3421 atcaaaatca atatccatga agaaatccca atgaaagaga ttacaagtaa cgcatctttg
     3481 gcactaggag aagcctatat ggatcatcga atcgagatcg agggaagtat ccaagcattg
     3541 atcaacgatg cttatcagca agcaaacagc tttttacgag gaaaagatta tctaaaatgg
     3601 ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca tgcgcattat
     3661 gacttaggta acgactttta taaactttgg ctagatccaa ctttgactta ttcttgtgct
     3721 tatttcaata cacctgaaga tacgttggaa caagcacaaa tcaataaagt ccatcatatc
     3781 ttagacaaac tctttatcaa agaaggcgaa acgttgcttg atatcggttg tggctgggga
     3841 acattgatgt tcactgctac caaagagtat aatgtcaagg caactgggat cacactaagt
     3901 gaagaacaat acgatttgat taccaaaaga ataaaagaag aacatttaga agataaatgc
     3961 cgtgtattat taatggatta ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt
     4021 gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag gttatttcaa agtggtaaaa
     4081 gatttattga aaccaaaagg aacagctttg atccatggaa tcagccgaca acaaggcggt
     4141 gcgacaaatg cttggatcaa taaatatatt ttccctggcg gttatgtccc tggtgttact
     4201 gaattagttg ataatattac aaaaaacgat ttgcaactga ttgatttgga aagcttacgt
     4261 cgtgactacc aattgacatt agaacattgg acaaagaatt ttcatcaagt ccaagatcaa
     4321 gtcacgaaag aaatgggtga ttccttctat cgaatgtggg atctatattt acaagcttgt
     4381 gcagcttcat ttgaatcaag taatatcgat gtgattcaat atttgcttgt tcaacctgga
     4441 aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt aaagactatt
     4501 taaaaagaag ctaagtttta tgatgctcca gaagtgaact tttttgtcta acttctagga
     4561 ttgtctctaa agcttagttt ctttttcttt atattttcat aataaaagtt tggttcttta
     4621 atttttaaac atgacagaaa attttttctt cgttatactt gtaatagaat actattttat
     4681 aacaaagggg atcgaatcaa tgaaaaaaac tgttatttat actagtttat ttgcagcttt
     4741 gtttttagct gcttgtcaat caaataccaa tcaaggcaca tctgggaata cgtcttccag
     4801 tagcgaacag cgctcttcac aggtatcgga ctcctcaaca aaagcaagca catcttcgtc
     4861 tagccagaca aaaagttcta gtgaagcaag tactcagtca gatatcaaag tgtctgcagc
     4921 tcgtgcgatc gaattgttcc aagataaata cccagatact gcaattacta gtttggaatt
     4981 agatagtgat tggggatctt acttctacaa gatagaaggt gtcgatgatc aaaaagaata
     5041 cgaagcaaga attgatgctc aaaatgaaaa aatggagaca gagaatccag aaactctaga
     5101 tcgagatgaa caaaatggtc aaaagaaaaa agaagatggt ctggatgttg ataaattgat
     5161 cacgattgag gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta cggcaacaga
     5221 ttgggatttg gataaagatt taggtactac ttattgggaa gtcaaagtaa aagacggcaa
     5281 tcaaagtaca tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa ctgaacaaga
     5341 tgattaagaa tttcttcgga aaacaagagt tgatttttct tttagtcatg atacactaga
     5401 caaaataact tgtttgaagg agcgatttac ttgagtttag aaacgacctt tgccaatcat
     5461 atctttgcca atcctttgat gaatgcatct ggtgtccact gcatgactac ccaggaatta
     5521 gatgaactgg ctcattcaga agcaggagca tttatcacta agagctgtac gatcaatgaa
     5581 cgaaaaggaa atcccgaacc acgctatttt gatgttcctt tagggagtat caattctatg
     5641 ggacttccaa acttaggttt ttcttattat ctagagtatg ctcttgcgta tgaaaaagtg
     5701 caggaaaacc aaaatcaacc gttatttttt tccattgccg ggatgagtgt ccaggaaaac
     5761 ttggaaatgc ttgagaaaat agaaaaaagt gggtttaatg ggatcactga attaaattta
     5821 tcttgtccca atgtgcctgg aaaaccacaa ctagcttacg attttgaggc cacttatgaa
     5881 acactgaaag aagtattcag catcttttct aagccgctag gaatcaaatt gcctccttat
     5941 tttgattttg ctcattttga tcagatggct gatatcttga atcagtttcc attgacctat
     6001 gtcaatgcta tcaacagtgt gggcaatggt ttgtatatcg atacagaaca agaagcagta
     6061 gtcatcaaac caaaagaagg gttcggcggc attggcggag agtatatcaa gccgactgct
     6121 ttggcgaatg ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat cattggtaca
     6181 ggtgggattc ggacaggaca agatgcattt gaacatctgc tatgtggcgc aagcatgtta
     6241 cagattggta ccgagcttca taaagaaggg ccggaaattt tttctagaat cataaaagaa
     6301 ttaacgcaga ttatgtcaga aaaaggatat acgtcaattg acgaatttaa aggcaaatta
     6361 cgcaccattt catagcaaag agacgaaggt catgattgat cttcgtcttt ttttataaaa
     6421 aacactcgtc tgttcattcc tgtgcaagcg tactcattgt atgttagacg aacttgtggg
     6481 aaaataacag aggcaaaaag gaggataata tgaatgatgc aacagaatta gcaaaagagc
     6541 ttaaagaaaa agaatttagt tttcaagaac tagttacgga tatacagaaa aaaataaaga
     6601 agcaaaatca tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt aattcccacg
     6661 agctattgat tccccagtca gaaaaagggc cattggctgg tatcccattt cctttaaaaa
     6721 tgctcggtca agaaaaaaag ggatggctag ctacttcagg atctcgttta tttgaaacac
     6781 accgagcttc tcatacatct aattatgtgc ggcaagcaga agcgataggt ctagttcctt
     6841 ttggacaaac caatgcgcca gagtttggtt tcaaaaatat tacagaccca gtcatctatg
     6901 gcccagcaag aaacccttgg aaccttgaat gtacaccggg cggatcaagt ggaggagctg
     6961 ctgctgcagt ttcatcagga atcgttcctt tagctggtgc tagtgatggc ggtggttcta
     7021 ttagaatacc cgcttcgttt tgcggattga ttgggctgaa accttcgcga gggacgatgc
     7081 cagttggtcc atatgcttgg agaggatggc aaggagctgc tattgatttc ggcttgacag
     7141 tttctatgag agacacggag gctttatttt atgggatgag aagtatccat tccggtgcgc
     7201 catatcaagt atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg ttgaagatag
     7261 ctttttgtgt tgattcaccg atccactcac agattagtga agaggcagta cttgctgtaa
     7321 aaaaagctgt agatttcctt ggaaaagaag ggcatgattt gacagagatt ccttacccac
     7381 tagatggtcg taaactgatc acgtcttatt atcagatgaa tggcgcagaa acagcagcaa
     7441 tgatccgctc gattgaaaca ggcatccagc gagctgtcag aaaagaagaa cttgaaccca
     7501 tttcttggac tttgttccaa tatggtcaga agctctctgc cgctacttac atcgattctc
     7561 tgcatgtatg ggatgaagca gcaattgtca tggaaaacct acatcaagaa tacgatctat
     7621 tcctttcacc aacgactgca tttaccgccc ctaaaatcag cgaagatttg caaagtgatg
     7681 cgataagagc acagatggaa cacatcagtg attgttctga agtagaaggt ttagacgttg
     7741 tttcttccat gtttgaggag agcttgaaga tcacacctta tacacagttg gctaatctga
     7801 ctggacaacc agcaatcagc ttgcctactc atttgacaaa agaaaacatg cctctaggaa
     7861 tccaatttat ggctgccaga ggaagagaag atttgctatt tcaaatcggc tatctatttg
     7921 aagcaaatca tcagttccat ttaccaaatc catggaaata gcagaaacaa ggagaagaag
     7981 aatgaagaaa aaaagattgt ttgctctaat aactagtgcc ttttttattt taagcgcttg
     8041 cggtacagct gccgctaatc aggaagataa cagctggagt aatgtagaaa aaaagaaaga
     8101 attagtagta gcgacttctg gcacactgtt tcccgcatct tattataatg atcaaaatga
     8161 attagttggt tatgatgtag atttggcaaa agaggtggct aaacggttaa atgtcaaaat
     8221 ctcctttaaa gaatacaacg tggatggaca aatcacttct gtttcaaaaa gagaatcaga
     8281 ttttgctgcg aacgattttg gtatcacaaa agaaagagaa aaaaaattct ccctttcaga
     8341 gactttcaaa tattcatttg acagtatgat cgtacgaaaa aaagatgatt caggaatcca
     8401 ctcacttgaa gatcttaaag ggaaaaaagc agctggcgaa cccaatacta gctatatgcg
     8461 tttagcggaa aaacacggag ctaaattagt cacatatgac aatgccacga atgatcagta
     8521 tttaagcgat gtagcaaatg gtcgaacaga tgttattcta aatgattatt atctgcagaa
     8581 aatggccgta tctgctttac cagatatccc tgtaaaaata ctggagaacg tttatttcaa
     8641 tgaaaacgaa agtggttttt tattgaaaga aggaaatgat gctctgaaaa agaaaatcga
     8701 tggcgtatta aaagagatga aagaagatgg gacgacaaag aaaatatctg aaaaatattt
     8761 tcaaactgat gtttcagtca aaccgaaaga aacgatggct gagtcggtga gtgagtgatg
     8821 tactttcctc taattgattt ttccctcatg tggacttcac ttcctttcgt actgcaaggc
     8881 ttggt
//
LOCUS       DACEZN010000112         8880 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEZN010000112 DACEZN010000000
VERSION     DACEZN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.112"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            243..1550
                     /locus_tag="IXH60_002071"
     CDS             243..1550
                     /locus_tag="IXH60_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1990978.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            1669..2868
                     /locus_tag="IXH60_002072"
     CDS             1669..2868
                     /locus_tag="IXH60_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1990979.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(2895..4163)
                     /locus_tag="IXH60_002073"
     CDS             complement(2895..4163)
                     /locus_tag="IXH60_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1990980.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(4326..4973)
                     /locus_tag="IXH60_002074"
     CDS             complement(4326..4973)
                     /locus_tag="IXH60_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ1990981.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(5381..5884)
                     /locus_tag="IXH60_002075"
     CDS             complement(5381..5884)
                     /locus_tag="IXH60_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1990982.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(6007..6771)
                     /locus_tag="IXH60_002076"
     CDS             complement(6007..6771)
                     /locus_tag="IXH60_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1990983.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            6878..7153
                     /locus_tag="IXH60_002077"
     CDS             6878..7153
                     /locus_tag="IXH60_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1990984.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            7869..8819
                     /gene="yidC"
                     /locus_tag="IXH60_002078"
     CDS             7869..8819
                     /gene="yidC"
                     /locus_tag="IXH60_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1990985.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         2702 a         1830 c         1648 g         2700 t
ORIGIN      
        1 tcttatgcat ttaattatga ttttcagttt attaagtatt atcagtttat ttgctttcag
       61 cgcttcccgc ttattagatg cggaaacacg gcctattcct gtaaaagtac gtaaaaacga
      121 ccgctaaact tccataacgc gcgttcttat cctaaagttt tttcagaaac attgaaactc
      181 tctcccctct ctgataaaat tttaatttgt agcgaaacaa attaattaca aggagatcgg
      241 atatgtccat aaaaaaaaca cgtctagtcc tattattggc tagtctcatt ctttgccttg
      301 cttcttttct accagctgtt gctgtgcaag ctgaagatac gttcaaagta aatgcaaaag
      361 cagcttttgc tgtagatgcg caaagcggta agattcttta tgatcaagat ggagaaaaaa
      421 ccatggggat cgcttcaatc acaaaaatca tcggtcttta catcgtcctt gatcaagtaa
      481 aagaaggaaa actgtcatgg gatgataaag tttcgatttc agattatgca gaaaatttga
      541 gtatcacccc agatttatcg aatgttcctt tgcataaaga aaacacctac actgtcaaag
      601 aattattcga ctctgcaatc atccagtcag ccaatgcttc aatggtggcc ttggcagaaa
      661 aaatctctgg cagcgaagct aaattcactg aacgaatgaa agaacaattg aaagattggg
      721 gaatcaaaga tgcaacaatc gttaatgcat caggattgaa caactcttat cttggcgaaa
      781 accgaccaga aggcactgga gaaaacgacg aaaatcaaat gtctgctcaa gatgtggcca
      841 tcgttgcccg ccatttgatc ttggatttcc cagaaatact ggatgtatca agtactacga
      901 cccaaatgtt tggcgaaaat acgcaatcac cagtggaaat ggtcaactgg aactggatgc
      961 tcccaggatt cgttaattac aaggaaggcg tggacggatt aaaaactggt acgactgagc
     1021 ttgcaggtgc ttgtttcgtt ggtacgatca ctaaaaacga tcaacggatc atcactgtcg
     1081 tattgaatgc ggacggccat gcggaaaacc cgagtgcccg cttcaatgaa acagaccgtt
     1141 tgatggacta ctgctatgac aattggtcag aaaaagagct cggaaaagct aacgcaagta
     1201 ttcctagtct aaaaaccatt gatgtaaaag acggaaaaga atcaacagtt cctgttgtgc
     1261 ttaaaagccc tgtaaaagta tgggtacgaa acgatatgga tactggcaaa ttaacgatca
     1321 cgccaacact tgacaagaag caagtcactg acaatgagat ccaagcacct atggataaag
     1381 gtacaaaaat cggtaccgct acgatcacct tggcagacga taaattaggc tatttagaag
     1441 acaacaatgc accatcaaca gagatcatta cggataaaac agtggaaaaa gcgaatatat
     1501 tcgtgattgg ctggcgacgc gtcgcagaat ttttcgacaa cttgttttaa ttgaacaata
     1561 agaaaaacat ctttccatcg gaaagatgtt tttttgcttt ttctagttta aataacagct
     1621 tttttcatct attttcgtta tactgtataa gaaaaaggag ttgaatttat gaatctaacc
     1681 agagatctga tctttttagt tctctatctt attggggtat tctctatcgt ttattggcga
     1741 aaacagaaga aggatccttc cttgttcttt tacggcagta cgtggacatt tacggtaatc
     1801 ttaacttatt ttgtcgtgtt ggaaattttt tattcgacct tttatttttt gattccattg
     1861 ttgttcttta gtatctttgc tttttcttat ttcacagaaa aacgaaggct gctgaatggt
     1921 cttttattca atgttttcct catcagtttc ggcatctact tgtttgtctt gctgtatgaa
     1981 acgcaaaata ttttccttgg cggactgatc gccttgatca cgattccatt gctacttgtc
     2041 cttttatttg gtatttatgg attgattgtt tttctttttt ggaacggggt cacagtttta
     2101 cgtagagaat cgcattcatt agctaatctt ttaacgttga tactagccat ttttcttaca
     2161 ctgtttcttg tttttgactt tttcttgtta aaatatttgc cgcaatggat aaatgctttg
     2221 tttttctgcg ttcccctgat cttgatctat ctatttatcg tattctacaa ctttttgact
     2281 gtctcattct tgtatcagtt caaccggcct cgatacaatc aagacttcat cgttgtgtta
     2341 ggcgctgggt tgatcaatgg agaaacagtt tctcctcttc tagccaagcg aatcaataag
     2401 gcaattgctt tttatcgggc tcaaagccgt gctacattaa atccgcccat cttattgatg
     2461 tctggcgggc aaggagcaga cgaaaaagta ccagaggcca ttgcgatgaa acaatatgcc
     2521 atggaacaag gaatccctga aagagacatt cttgtcgaaa ccaattcgac aaccactttg
     2581 gagaacatgc tctattcaaa ggaaatcatg gatcagcaaa tgaaagggcc ataccgcgcg
     2641 atctttagtt ccaacaatta ccacattttt agagccggtc tttatgcgtg gcaagccaaa
     2701 ttgaaagcaa atggtattgg tgcaaaaaca gctttctact acttgcctaa tgcgttttta
     2761 agagaataca tcgccatctt gatgatgaac aaaaaaagac atatgatcat ttgcggattg
     2821 cttttcgcag gctccgcatt cttatcgctg attactttac ttttctaaaa aaacaaggtg
     2881 tgacaaaaca tatgtcacac cttgtttttt tctttcattc tcttaactat tggcaaaaag
     2941 agtatccctt ttttgaccgc tacttgctgg gcagaataaa taccgctgtt gcctgaacac
     3001 ataaagctga tcaagcagat cataaagaag tatactgccg cttgtgaacc gaacaattcg
     3061 atccccatca caaagcaggc aatcggtgta tttgtcgcac cagaaaatac tccgataaag
     3121 cccaatcctg caagaaaagg aacagaaaca tgaagcaaag gcgctagtgc tgcaccaagc
     3181 gttgcaccaa tttcaaacaa tggtgtcact tctccccctt gatatcccgc gcctaaagac
     3241 aaaactgtga agaataactt cccaataaaa tcatagacat gtgcctgtcc attaaatgat
     3301 tcttgaagta acggaagact caatccgaga taacgttgtg tttgcaaaat caatgctgct
     3361 gccacaacta cagctccacc tatcgtattt cgccaaacag gatgaggaat ccattgtgca
     3421 tatcgcgctt ttaaaaagac gatcgaacgg ctgaataccc aaccaatcaa accaaaagca
     3481 atcccagcaa caaaaacttt gaagaacagc aacatactcc attcaggaat cacacccatt
     3541 tggtaatgta gatgagaaac accatagctt tctgtcatga aatttgcaaa cagtgcagca
     3601 aagaagctag ggaagatcgc ttctgttcgg actttcccga ttgccaacac ttctaatcca
     3661 aataatgttc cagctaaagg tgtgccaaat acagaactaa aacccgcact gattccactg
     3721 ataatcaaga tttcccgttc tgctttatcc aatcggaaca aacgtgctgt attatctgct
     3781 aacgatcccc ccatttggac tgctgttcct tcacggccaa cagacccccc aaataaatgg
     3841 gtagttatgg ttccaaacag tgtaagtgta ataagccgca acgggatttt ttcttctcca
     3901 ccatttcctt gatcgatcac taagttgttt ccacgactag aaagaccgcc gtgatacgca
     3961 tagagataag caaaaagcgc accactaact ggtagtagat aaaggagcca aggatgtgat
     4021 agtcggatat ctgtcacaac tgtcaggcta tttaagaaaa aagcagacaa gctccccatg
     4081 aatataccga gaatcgctgt gatcaccagc cacttcagca tgtacactaa aatgcgcatt
     4141 ggactcggta attgtttttc cataaaaaat tcctcctcaa taaaaaaacg cctactaaag
     4201 aaaggggctg tccccagact ttagtaggcg tcattagcta tctttcgtta gcatttaaag
     4261 gcgaacacca tcacctattc acttccatta aaagatagca tatttttctg taaaaaacaa
     4321 taaaattatc gttctttctt tacacgttca gcaaattcat agaaaggagc caatttcgtt
     4381 aactgttgat tacattggtc actatcatga caaatataat tccccttttt cgtatacgtg
     4441 ccatcagaac ctgctttcgt tgtagaaaga aaaagcgaca cattcgatgt tttctgacag
     4501 attgcacaga ttcccttatt tatcgttggt gataaagtac cgctaatccc ttttaatttt
     4561 tcatcttcgt aatagaccat gaattttttc tgcgtacctg gatcattcca gccgatgtac
     4621 gtatgttctt gcaagtcgat ttcattccaa gctggttgtt tgagtttttt tacttttcgg
     4681 aataatttct cgatttgtgg tttgctaggc tgcataaacg gctgaacgat ttgcttcagc
     4741 ttctccaatg ctctttctgc ttgcgctttt gtcagtttta cgtctaaaac tgtcgttaaa
     4801 aatgtatcga tttctggcag ttcgtgattt aatatgtctc gaatcttttc agctgctagt
     4861 gcctgaactg tttgtaccgt ttttggatca ttgacagatg agtacgcatt taataactgt
     4921 tctacttctc tttttataaa aaaatattgg tatggtttta ttgtttgttc catgatttta
     4981 aagtcctttc ttctaaccaa agtgcatact ttgtagaaaa aagtgatctt ctgcaaagta
     5041 tgtgttacct gaagatcccg gactctttta aataagatac gttttgcatc ttatcatcac
     5101 cccctttaat gcttattctt ctatgtacat gattcattcg ataaataaaa gtaaaccgta
     5161 tcttgaacag atagtcaact gcaaacagaa aaaaggactg tgacaaaagt cgtgtcaaag
     5221 tcccttgttt cgaatgaatg gtgttcaaaa gcttgcatct gcggtaataa gccgaaactt
     5281 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
     5341 ctgtcacaac ctcaatatgg tctattattg gttttacctg ttaaatcgag gattctttcc
     5401 caaagtaacg ttgccatctc ttacgcatac ttgcagacat gggctgagcc gcttctttga
     5461 ctgcgcagta tttgtccacg aatgtcacaa tatatccttc tttatactta ggcggtgcga
     5521 tcgttgcacc ccacatgtgc ttcaaaatga tatctcgctc aagatccgac aaatcggtga
     5581 ttttttctgc attctttaca gcaattcttg gatggatata cgcatgtgtc ccctcgtcaa
     5641 atttcgtcgt acgccaatca tagtagaaca gatcatgcaa aagtccagca cgcgctgttg
     5701 ctttagcatt gccaccccat ttttttgcga gcagataact atagtaagaa acactgatgg
     5761 aatgctccaa acgagtggaa tgaacatgct gtgtgtaatt cgctaatttt ttcactgctt
     5821 ctgtttctaa aagatcttgt acataagaca tatattcttc gtcttcacgc catttttctg
     5881 tcataattat cgatctgcca cctttttgaa tttcattttc ttctgaaatt gatctaagta
     5941 taccaccagt gttcggatta ttctagtcgc tctagataaa atagctaaag cttaatagtt
     6001 ccttaatcat aaagaaaaat gataaagaac gatccctgct gcgactccga cattcaatga
     6061 ttccgcattt cctttcattg ggatatatac gttctgatca gccgaagaca agatttcctg
     6121 acttactccc tgaccttcgt ttcccatgat tatagcgaaa tcctctgttt tttctacttt
     6181 gtctaatgca tgtgcttctt catttaattc tgtaccgtac accgtaaacc ctttgccttt
     6241 cattacaggg atcacttctg ccaaatcttt ttgtaacaca ggcaaatgat aattgcttcc
     6301 ctgcatgctt cgcagcacct ttgtactata aatatctgcc gttcctttac ctaataaaac
     6361 accagccata ccaaacgcat ctgccgtacg gatcatcgtt cccacatttc caggatcctg
     6421 tacattatct agtaaaagcc agcctcctga aaaatctggc gtatcttcag atgttaacgt
     6481 catcactgct aagatccctt gcggtgtagg tagatctgac atagcggtca tgacctcatc
     6541 tgaaactaga aaagtagaaa tctctttgtt tacagccagc cagtctcccc attctttcat
     6601 acctctctgt gtcacaaaaa gttcttttat ttctgcatca gacgacgcag cttcttcgat
     6661 cagatggaac ccttccaaaa gatattctgc cttttcttct ctgtatttct ttttatgtag
     6721 tttttttaat tctttgattc ttggattttt tactgattgg atctctttca cattcctcac
     6781 ctcaactgat tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta
     6841 tcattgattt ataaaacagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaatgt
     6901 ccaaggacgt gtccagggcg tgggatttcg ctatatgacc aagatggttg ctgatcagtt
     6961 aggcgtgacc ggaacggtaa aaaacgaaga cgacggctca gtcacaatcg aagcaatcgg
     7021 aaatgacgac atcatccaaa aatttatcga agaagtcaag aagtctccga gcccaagtgg
     7081 acgagtgcag tatgtggatg tccaagaaga tccgatgatt gaggaacgaa agaaatttga
     7141 tgtggtggga taaggttgtt tttgaagcgc tttgcctcga gtattaagga gaaacatttt
     7201 ccaaatagct tctcatattt tgaaatgtaa tgacttaata ccgagaggca gctgataaaa
     7261 caccgtcaat aaggttgtga aaaagctggt ctgcatcaag cattaaggac tggttgtgaa
     7321 aaatagctct ccatattttt caacaggctg gacttaatgc cgtagatgca agcttttgaa
     7381 caccgtcgat aaggttgcga aaaagctgat ctgcatcaag cattaaggac tagttgtgaa
     7441 aaagtcgctt agttttgagt attaaggaaa attttgtgaa aatggctttc cacatttttg
     7501 cataaatttg acttaatacc aagaaactga cttttgaaca tcgtcgataa ggttgttttt
     7561 gaggcacttt gcctcaagta ttaaagacag gtagtaaaaa agctgatcta taacttgcaa
     7621 taaatttgct taacccatat tttccagaca gaacaaaaaa gtttttagtt ttttaaataa
     7681 gaaagaaaca cttggaatca ctcttttctt tctaagtatt tctttcttga ttttttgtct
     7741 tttttcaaaa tttttcgcta cgaaaaagaa cgaatattta tatgaaattt ctatgaagtt
     7801 gattgaattc catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga
     7861 agtattctat gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc
     7921 tctttttatc tggctgtgtg cggatggatt caaacggaaa ccctgacact tcaggaatcg
     7981 tttatcgagt actcgtccat cctatgggcc aagcgattac atatctagtt gagaatttca
     8041 attggtctta cggctgggca gttattgcaa tgactgtgat cgttcgtatc atcatcttgc
     8101 cactaggtat cagtcaatcg aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa
     8161 aaccacaagt tgaagcagca caacaaaagc taaaagctgc aacaacacgt gaagaacaaa
     8221 tggcagcaca agcagaaatg cagcaagtat atcgcgaaaa tggattgagt atgaccggcg
     8281 gaatcggctg tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tattttacgg
     8341 ctcgctacac agaaggtatc cgtgaatcat ctttttacgg catcgatcta ggctcaccaa
     8401 gtatggtgct tgtggccatc gcaggggtcg cttatctgct tcaaggatat atttcaacaa
     8461 tcggtattcc tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta
     8521 tgatcgtctt catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtcggtg
     8581 gtgtgttcag ctgtctgcaa acattcatca caaacgttat aatgaaaccg cgtttgaaag
     8641 cacaagtggc agaagagttg aaacaaaacc cgccaaaaca agtcgttact ccaagaaaag
     8701 acgtgacaga atcagcaaag ccagctgaca acaaacaagc aacaaaacaa atcaaacaac
     8761 caaaaaacag tacaaacaac aaaggccgca acgcaggaaa gcagcagaag cggaaataaa
     8821 aatatatgac aaaaaactgt agacaaataa aaaaatttct ttgttctaca gtttttttgt
//
LOCUS       DACEZN010000113         8721 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEZN010000113 DACEZN010000000
VERSION     DACEZN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.113"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            2..74
                     /locus_tag="IXH60_002079"
     tRNA            2..74
                     /locus_tag="IXH60_002079"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IXH60_002080"
     tRNA            78..150
                     /locus_tag="IXH60_002080"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IXH60_002081"
     tRNA            155..225
                     /locus_tag="IXH60_002081"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IXH60_002082"
     tRNA            238..311
                     /locus_tag="IXH60_002082"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IXH60_002083"
     tRNA            323..394
                     /locus_tag="IXH60_002083"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IXH60_002084"
     CDS             660..1853
                     /locus_tag="IXH60_002084"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1990986.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IXH60_002085"
     CDS             1983..3455
                     /locus_tag="IXH60_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1990987.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IXH60_002086"
     CDS             3538..4326
                     /locus_tag="IXH60_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1990988.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IXH60_002087"
     CDS             complement(4393..5061)
                     /locus_tag="IXH60_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1990989.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IXH60_002088"
     CDS             5176..6888
                     /locus_tag="IXH60_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990990.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="IXH60_002089"
     CDS             6891..8663
                     /locus_tag="IXH60_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1990991.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2618 a         1486 c         1940 g         2677 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt t
//
LOCUS       DACEZN010000114         8627 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEZN010000114 DACEZN010000000
VERSION     DACEZN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.114"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(53..886)
                     /locus_tag="IXH60_002090"
     CDS             complement(53..886)
                     /locus_tag="IXH60_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1990992.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1190..3355)
                     /locus_tag="IXH60_002091"
     CDS             complement(1190..3355)
                     /locus_tag="IXH60_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1990993.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3615..5909
                     /locus_tag="IXH60_002092"
     CDS             3615..5909
                     /locus_tag="IXH60_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1990994.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5902..6567
                     /gene="pgmB"
                     /locus_tag="IXH60_002093"
     CDS             5902..6567
                     /gene="pgmB"
                     /locus_tag="IXH60_002093"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1990995.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6560..7567
                     /locus_tag="IXH60_002094"
     CDS             6560..7567
                     /locus_tag="IXH60_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1990996.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7589..8608
                     /locus_tag="IXH60_002095"
     CDS             7589..8608
                     /locus_tag="IXH60_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1990997.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2635 a         1874 c         1592 g         2526 t
ORIGIN      
        1 cgtttggaga gttttatttt aatcagtttt tttatgttat atgttatatt ttttattcgt
       61 tcacatctaa ttcgatttcc accccgtagt gatcactgat gattggacta tcgatcccat
      121 caaaaatccg ttgatatttt ttaacctgca ttccttcagg gacgaaaata aaatctatac
      181 gcaatttttc tgtattttgt tcccaaccat ctattttctt ttccacagta gctgcttttc
      241 ctttttcatt cgcgacaaca tacgaatctt gaagaagaag gcaactttct cttacagtat
      301 aataaccttg agagtctact ggattattga aatcacctag gaaaaaaagt ggtacgcgag
      361 tttccaaaaa gtagttttcc aagtttttcc actcaaaata aaagccactt tttttatctg
      421 tccaccaaga aaagtggcag ctcgagacaa aaatgtcctg atcatctatc tttgttttcc
      481 caatcaagat cttgcgtgtc tgataatctt ctttcttttg aaattgggag acggtaagga
      541 cctgactttc caatgttttt ttcgtaagca gtgcatttcc ttcttcatat ttatcataac
      601 cgatgtggct catttcccaa ctccaaaaat actcttttcc gtgttccttt aaaagctgaa
      661 ctaaacggta ggcaaaatta tctttacgga taggtacttg attctttacc ggacagaact
      721 tcattagctt cgcaggttct acttcttcag attccagtaa ttggttgatt tcttgtaaag
      781 caatcacttc ataatcttct aacaggatct tatctgctaa ttcttgcaat tttttctctg
      841 gttctggctc aagccagcta tgcgtgttga gtgtcatgaa tttcattggt tttctccttg
      901 tcttcatttc gaaacaaaag ggttgtgaca aaatttttgt cacaacctct ccagcaaata
      961 gtgttctcct gctaactcct cagtaataag ctcaatggac caggagctat tttttgtttg
     1021 gctgttttta tcacttggtg cagggcagct ttttcacgac ctctccacta aacagtattc
     1081 aaactgctgc ttctcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt
     1141 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctcct tacattttaa
     1201 tggagccgat cactttacca gctgtcactg tacctaattg cttgatcact acttcttcta
     1261 ctttatcgtt tgtaaatacg atcatgatcg ttggatcttt ttcttcgttt tggatcacat
     1321 caagatccat ctcagctaat ttcgttcctg ttcctacttc ttggccttct ttgacagcaa
     1381 tctcaaaagc tggctgattc atttggactg tatcgattcc catatggatc aaaacttcga
     1441 taccatcttt cgtttccagc ccaattgcat gtttgctagg gaaaatactt gtcacttttc
     1501 cagcgatcgg cgcaacaact tctcttgatg ttggaataac agcaaagccg tcacccatca
     1561 ttttttgtga gaataccgga tcttttactt cagataaagg aatcacttct ccagaagctg
     1621 cggaaactag ttcttgttct atacctaagt attgcttact acctgcttgt ccatttcctg
     1681 tagaaaccat ttctggctct ggaatctctg ctcctgattg cagcagatct tcgatatctg
     1741 atttcaagac atctgctttt ggtccataaa ctgcttggac tccattgtct ttgacgatca
     1801 atcccatcgc ccctgctcgt ttccaggctt cttcagtccc tactttttct cgatcattta
     1861 cacttacgcg taaccgagtc atacaagcat cgacatcttt gatattttct tttcctccca
     1921 gcaaatgaat gatctggacc acttgctgat ctgctgttcc tgttccgctg ttagatacaa
     1981 cggtttcttc actgtcattg tcgtaatttc cgttacgtcc cggtgtagca aagttgaatt
     2041 ttttgatcaa gaaatttgct aagaaatacg taacaacacc aaaagcgatc acacacaaca
     2101 cgaagttgat aagatctcca cctagacccg ctttgatagc taacggtgtc cgagtcagca
     2161 attcgatatt cccaaatgaa tggacccgca atggcaagat atctgccatc gcaaatgcag
     2221 ccccttggat cactgaataa atgacatata atggcacagc agcaaacata aacatgaact
     2281 ccaatggttc tgttacacct gttagaaaga cagccaacgc tgctgaaaaa tacattgatt
     2341 tatatcttgc tttcttatca ggatcgacat tacgatacat cgcaaaagcc attcccatca
     2401 agatacctga agaaccaatc atttgtccta ctttgaaacg agccggcgtc cagttatgca
     2461 acacaaactc atattgcgac atatcccctg cacctttaag attcactaaa tcagtggccc
     2521 aagccaacca taacggatct tgcccaaata cttgtgtccc cgcctgtgcg cctgataaaa
     2581 tctcatacga tccgccaagc tgtgtatagt tgataggaat cgtcagcata tgatgcaatc
     2641 caaatggcaa tagtaaacgt tctaatgttc caaataaaaa cggtgctaga atcggtgcgc
     2701 tttcttgtga ttctgcaatc cataaaccaa aattattgat tccagcctgt acgtttggcc
     2761 acaccacagc aagaatcaat gccacgattg ttgaccataa gataacaaca aacggaacaa
     2821 agcgctttcc attgaaaaat gacaaagcat ccggcaattt tctataatta tagtacttat
     2881 tataagccat tgccccgaca aatccagcga tgatccctac gaatactccc atattcaaag
     2941 ctggtgcttc caatacactt gtaaaaaatc ctttaaccat gatcttcgta ccgaacagag
     3001 tgtgggtaaa tgcatcttca ttcgcaagca tgtcacttgt gacaccaaag atagccccag
     3061 tgatccgatt gattaaaata aatgagatcc cagctgcaaa tgctccgcct gctctttctt
     3121 ttgcccaact tcctcctatt gctaaagcaa ataaaatatg aagattacca ataattgccc
     3181 aaccaatatt ttcaattact ccgcccgttg tgacaagcgg cgtccaatca ggattgatca
     3241 agggaattga tttccctata ctgatcatca acccagctgc cggcataact gctaccacaa
     3301 ccattaaagc ctttccaaat ttttgccaaa actcaaaact aaatatcttt ttcattccgt
     3361 ttagctcctt tacgcaatcg gtttcgttaa acgtattata gcgtgcgttt tcttttttgt
     3421 aaatgcttta ttttttattt ttctgtctat accattctat aaaacctata atacgtgtgt
     3481 tcccttctat aattttctcc taaaaattta tttttttaag cttttttcta tgaaatattt
     3541 tcttttgtct attgacttgt gcaatcggtt gcgtttatta tgaacgtata aaacagctca
     3601 ttaggaggag aaaaatgaaa cagatcaaac gactatttgc tattgatcca tggaaaatca
     3661 gcacgacaca attagataaa aaaaatcttc gactgcaaga atctttaaca agtattggaa
     3721 acggttatat ggggatgcgc gggaactttg aagaaactta ctcaggggac caccatcaag
     3781 gtacttattt ggcgggtgtc tggtatcccg ataagacacg tgtcggctgg tggaaaaatg
     3841 gttatccaga atactttgga aaagtgatca acgccttgaa ctttatcgcg atagatattt
     3901 cgatcaacga tcagctgatc gacttggctt ctttggaacc agaagacttt tactgggaat
     3961 tagatatgaa aaatggtgta ttatctcgta cattcacaat cactacttct actaataaag
     4021 tacgcatttc cttcgaacgt tttctgagca ttgtcaaaaa agaatcagct tatatctatt
     4081 taactataga aatgcttgaa ggtagcggaa cagtcaaact cgtatcaaaa ttggatggaa
     4141 atgttcaaaa cgaagacagc aactatgatg aacatttttg gaatgagatt gatcgtggaa
     4201 ctatagaaga aatcggctat ttgacgacta aaactattcc aaacaacttt gggattgaac
     4261 aatttactgt tactgcagca atgcgccatt tcgttaatgg aaaatcagtc atccctgact
     4321 atcacgaaga tactttagca ttacatggtt gcttgtctgc agaattatct caaggtgaga
     4381 catggtcagt ggaaaaagaa attatcgttc ttactagtag agatattcca gaagcgcagc
     4441 aagcagaaaa agctgtctca gaattaacga accagacaaa caattatatc caagcaaaag
     4501 aagagcaaac agcggcttgg gcagaacgat ggaagcttgc cgatgtggtc atcgaaggag
     4561 acgaagaagc acaacaaggt attcgattca atttgttcca actattctct acttactacg
     4621 gcgaagatga acgattgaat atcggcccca aaggattcac aggagaaaaa tacggcggag
     4681 ccacttactg ggatacagaa gcgtatgctg tccctcttta tcttgcttta gccaaacctg
     4741 aagtaactaa gaatctgctg aaatatcggc ataatcaact gccgcaagca atccacaacg
     4801 ctcagcaaca aggattaaaa ggcgcgcttt atcctatggt caccttcact ggtgtcgaat
     4861 gccataacga gtgggaaatc acattcgagg agattcaccg aaatggcgcg attgcctatg
     4921 caatctacaa ttatacgaat tacaccggcg atacggaata tttgaaaaat gaaggactgg
     4981 aagttttagc agaaatcgca cgcttctggg cagaccgtgt ccatttttca aaacgagcaa
     5041 ataaatacat gatccacggt gtgaccggcc caaacgaata tgagaacaat atcaataaca
     5101 attggtatac aaatacgatc gcagcttggg tcttgcgtta cacgcgagaa aattatttaa
     5161 cttatcaaaa agaagctgct atcactatca gcgaagaaga actagccaaa tggaaagata
     5221 tcgcagagaa catgtacttc ccagttgacg aagaattagg aatctttgtc cagcatgaca
     5281 cattcttaga taaagatctc atgcctgtat ccgatattcc cacaagcgat cttcctctga
     5341 atcagcattg gtcttgggac aaaatcctta gatcttgctt catcaagcag gcagatgtac
     5401 tacaggggat ttatttcttc aatgatactt ttacaaaaga agaaaagcaa aaaaactttg
     5461 atttctatga accaatgact gtccatgagt cttccctttc gccaagtatc cattcgattt
     5521 tagcagcaga attaggaatg gaaagcaaag ctgtagaaat gtaccagcga acagctcgct
     5581 tggatttgga taattacaat aacgacacag aagacggctt gcacatcact tccatgactg
     5641 gtagctggtt gtcgattgtc caaggatttg ctcaaatgaa aactgcccat gatcagttga
     5701 gattcgcacc atttctgcca aaaacttgga aagcttattc tttccatatc aattatcgcg
     5761 gacgtttatt gttcatctca gtcacagatg aacaagttac tttcgagtta ttagaaggtt
     5821 ctcctctaga aatttacgtc tatgaacagt cttattcttt agcggatcgt ctagaaataa
     5881 cgctaagaaa ggaggaagca aatgtttaaa ggggtattgt ttgatttaga cggtgtcatt
     5941 accgatactg ctgaattcca ttatcgtgct tggcgaaagc ttggtcagga aatcggcatt
     6001 acgatcgacc acacattcaa cgaacagcta aaaggcgtca gccgagaaga ctcactctct
     6061 ttgcttttag cttatggcgg aaaagaacac tctttttcaa aggaagaatt tgcagaatta
     6121 gcgaaaagaa aaaacgatta ttatttagag atgatccaga cgatcgaacc aaaagatgtc
     6181 ttccccgggg tcgtgccttt actggattct ttaaaagaag aaaaaatcaa gattgctctt
     6241 gcttctgcaa gtaaaaacgg gccattccta cttgaaaaaa tgggtcttac accttacttt
     6301 gatgctattg ccgacccggc agaagcaaca aacggaaaac ctgcgccaga tatcttcctc
     6361 cttgcagcaa aagctgtcgg actaaaagca gaagaatgta tcgggatcga agacgcgcaa
     6421 gccggtatcc aagccatcct ctcaagtggc gctcaaccaa ttggcgtggg acgtgctgaa
     6481 gaccttggag aggagatccc gcttgtccct gatacgactt ttcttacgat cgattacttg
     6541 aaagaaaagt ggcatgacca tggataatcc agagatctcc gtcgtaccag ttccagagac
     6601 tgaaataaag aaaatcacat tgaagaacga ctacttagaa gtcgttcttc tcaattacgg
     6661 tgcccgcctg caccaaatct tcgcaccaga caaagaagga aaatcagaaa atgttttatt
     6721 gtcttacgat cgtttcgaag atgttctaac agacaaaagc ttttttggag caactgtcgg
     6781 acctgtagcc ggaaggatcc gggatgcttc ttggggtgag catagattgg agaaaaactg
     6841 cggatcacac catatccacg gtggcacgaa aggctggtct ttccaatttt gggaagtaga
     6901 agttttcaaa actccatcat caataggtgt ggtcttttac ttaaaagatg aattttctac
     6961 ctatccagga ccaatcactg ctacgatcac ttaccgttta acaaaaaacg aattagagat
     7021 gacaacctct gctagtagcc ttgttgaaac catctgcaat ccgaccaacc atgcctactt
     7081 caacctttca ggaaatggaa caagagacat ctatgaacat cagttaacgg tctttttaga
     7141 tggtgtgctt gaattagatc aagaaaaact gcctactgga aattggacaa aaaaagaaaa
     7201 actgccaatc gactttagaa aatccccaac actgcaagag atccttgcat gttatccaga
     7261 tggactagat gatgtatttc ttttgcatca tcctaggctt tccaaaactt ccttacaatt
     7321 attcgagaag cattctggtc gccaaatgac tatagcaact tctaataaaa gtatggtatt
     7381 attttctacg actggattcg aagctgattt ctctgtcaat ggaaaacaga tgcattcaaa
     7441 ctacggatta gcaatcgaac cgcaagaaat ccctgatatc gttcatttcc ccaaatttgg
     7501 atcgatcaac ctccatcctg gtcaagaacg gatcagtcag acgatttatc ggttctctgc
     7561 acaataacaa gagaaaggtg tttttatcat ggctattacc gtaaaagatg tggcgaaaaa
     7621 agccggtgtt gccacctcga ccgtctctcg tgtcatcaac gatcatccta gtatttccga
     7681 gacaacaaag aagaaagtcc gaaaaattat ggatgaacta ggttatgttc ctaacatgac
     7741 tgcccgcaat ctaggaaaac gcatttccag tgccatcggc gtaatcttac cccctcttga
     7801 ttcaaaagag cggatcggta acccatttta tcttgagatc atggaagcaa tcaacgaaga
     7861 agcgcggaat tatgatatga ctacagcaat cgctactgct aaaaattttg aattgctgtt
     7921 agagaacgtc cagcgtatgc atttacaaaa acaagtagat ggttttattc tagtgtattc
     7981 agatagtcaa gatactgtca ttgattattt ataccaaaac cacatccctt tcactttgat
     8041 cggacagcct tatcatcatg agaacgaaat cgtttatgta gataatgata accagcttct
     8101 aggtaaacaa gcaacagaat ttttgatcca gaatggacat aagaatatcc tgtttgtgac
     8161 aaacacgact catgaaagtt tgtatttcga acgatatttc ggttatcaaa aagcaatgat
     8221 gatggctggt cttcctgcgt tatccagtgt agtactagag catccggagg attatctgtc
     8281 ctttgagaaa acgttgaaag aaaacgaagc cacagcattg gtggtcattg acgatatctt
     8341 cgcccttcgc acgatgcagt tggcgcaaat gtatggctat gtcattccag agacactctc
     8401 cgtcattagc ttcaacaact cgatcttctc aacattaaca cacccttacc ttacaagtat
     8461 cgatatcgat atctccgaat taggacgtgt cgctatgcaa aagctgcgcg aattgatcca
     8521 tgaagaattg agcagtggcg tacgtttggt catccctcat cgcttgatca aaagagagac
     8581 agtcgttcct ttagaaaacc catactaaat agctgactca ctaaata
//
LOCUS       DACEZN010000115         8562 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEZN010000115 DACEZN010000000
VERSION     DACEZN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.115"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1331
                     /locus_tag="IXH60_002096"
     CDS             <1..1331
                     /locus_tag="IXH60_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1990998.1"
                     /translation="IIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECGTSLFI
                     KGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKL
                     ILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYIGVEIA
                     EAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGKEKI
                     EKIVTTRDNKYAMDMVIQAIGFLSHSPIGKKEFLRDVSGAYLTDEYQQTNIKDIYAIG
                     DCATTFFTSINRSSVDFSISNALRTAYIATQHINDQSFTPSGSQFTNGFSIFGYNFYA
                     AGLNLKTARLLSEAIDYIEIEDYIRPIFSKTNNIVKLRISFEKKAKRIVGVQFYSQED
                     LSGLLTMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNSGA"
     gene            1443..2846
                     /locus_tag="IXH60_002097"
     CDS             1443..2846
                     /locus_tag="IXH60_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1990999.1"
                     /translation="MKKSLKLASAVLLMSHLLIATPFHVLASENSTYKIAKERTKTTE
                     MSNSDVTAEGSEFSTTSSSESSTTLNAESSTTSSSESSMTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTSQQIESTETFQSQPV
                     LSGNEEIHFDKNESVENFIMKIGDSARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGSFVIMVTNEDLGNGTLYKTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNGLIKTYDLTDYDKEVQGP
                     QMNKNGYQIPIHNYSISSLFGNRGDEFHRGLDMAAAQGEPIHAVKAGKVIKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVVQGQMIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGN"
     gene            3264..3479
                     /locus_tag="IXH60_002098"
     CDS             3264..3479
                     /locus_tag="IXH60_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991000.1"
                     /translation="MNQEIADIKLIKEVLQESTANAIASGSGISLSTAKKLKSGERNV
                     EKLNLSDAIKITEFAIKNRHVKIEIWK"
     gene            complement(3565..4476)
                     /locus_tag="IXH60_002099"
     CDS             complement(3565..4476)
                     /locus_tag="IXH60_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991001.1"
                     /translation="MKIKIERNQKEKSPKKNKERVISVGKHRKMVLALWLLLSCSLAF
                     GIYRNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWRNNKEAIEKRMSNL
                     EQYLTENGLALSQDMVRADIPTSSEVQSIKILDVEKHSEEFIVSFLVEQQITEGKKTQ
                     SISSAYRVTIFEDENGSHIVTSLPTMIAKPEKAKYESKQIGIESDIDAKTTEEVTDFL
                     ETFFKLYPSASEKELEYYVENKAVQPINSNLKLVELVNPTFKLKGKEIQGEIFVKYLN
                     NTTKVSNIFEFTLTLQKVKNWKIIDIG"
     gene            complement(4495..5499)
                     /locus_tag="IXH60_002100"
     CDS             complement(4495..5499)
                     /locus_tag="IXH60_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1991002.1"
                     /translation="MNVKKLSIISLTILTLSFMVVLMCVGLLFGEDSEGSNSSGASSG
                     GSNVSEEVLKHRAMVEKYAKESNISEYVLILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVAKNGKKHNFALA
                     ESFSKEKSSGQKVDYPNPIAIKKNGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGASPETGFDCSGLTQWCYAKVGLKLPRVAQDQYNAMTHIDVKDA
                     KPGDLVFFHSTYDTGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(5496..7619)
                     /locus_tag="IXH60_002101"
     CDS             complement(5496..7619)
                     /locus_tag="IXH60_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1991003.1"
                     /translation="MKKKLLRIVGIIAISLCTILLLLSLIGTVAEATGLVDDTVKSGN
                     LYSQYSLDNYQLDFFVDSSWGWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQVSGFYFGFLLLMILALGVYVAYI
                     GLLKREATKAVRAVINFVTIFLLSGSFIAYAPTYITKINNFSSDVSEAALSLGTELVV
                     PNSESKGKDSVDLIRDSLFSIQVRQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRIMRKPHMLMNRKLR
                     QLNRNVIRTLAFGGGAAVGHKLEKEQSKSKFKPSGSSLRKNSRLPNDHEVPSDSTKEN
                     PVSNNKKQSRMNLAGRKMGKVLDTQALVKDKTKQVRDQVRNTPTNLKYNFHKGIEKTK
                     KAPKEFKRGLVQEKANRAELREKQRQHRDEKMAEKRKVLNETGNRHKKRRENVSIKPE
                     RQPQKDRAPKKEAPKQIVRVNPNPEIKRKLSSHEIVSKRISQPSSKKNSFQQVKQRST
                     LPKRTNQKVQKRMNPRPKSRSGEKK"
     gene            complement(7624..8535)
                     /locus_tag="IXH60_002102"
     CDS             complement(7624..8535)
                     /locus_tag="IXH60_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1991004.1"
                     /translation="MASEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLT
                     GISSRDGEKFPVLRKAIRTVTQSNQRGLLQVITELRNDGTPVAENIADHIESFTDYDF
                     AHLLFSDGSITQSISLEKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFA
                     LDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLD
                     ERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDESNQKRLRDLENGQCLMQDLYGRV
                     GVVQVHPVFEELFHAFDTRPPVQKRME"
BASE COUNT         2801 a         1583 c         1461 g         2717 t
ORIGIN      
        1 taattattgg ttcaaaccat tcagggctag ccgcagctcg atttctaaac ttaagcgatt
       61 caaattatga agttattcta atcgataaaa aatcagaaat tagttacttg gaatgtggga
      121 catcgttatt tataaaagga gaagtaagta atttagaaga tcttttttat caaaatagaa
      181 tgacactaaa ttctgagata aaaaagttat ttctagagac agaagttgac aaaattgata
      241 ttcaaaataa aaagattagt tgtgtaaaaa aagatggcac aagattttcc caagaatatg
      301 acaaacttat attagctaca ggttcaaagc aaattatgtt agatataccg gggaataatt
      361 tagctaatat tttttcgata aagaattttc caaatgccaa agagattttc aaagctttag
      421 atgataatac tattaaaaaa gtaggtatta ttggagcagg atatattgga gttgaaattg
      481 ctgaagctat aagaaaaaga gggaaagaag tatatctttt cgatgttgca gatagagtgt
      541 tgtcgaccta ttacgaccgt agtttttcag ataaagttga agaaatacta tcaaaaaacg
      601 ggatacatct ttgtctatca gaaagtccta aaaagtattt aggaaaagaa aagattgaaa
      661 aaatagtgac tacaagagat aacaaatacg ctatggatat ggtcatacaa gctataggat
      721 tcctatctca tagtccaata ggaaaaaagg agttcttgag agatgttagt ggagcatatc
      781 taacggatga ataccaacaa acaaatataa aagacatata tgctattgga gattgtgcta
      841 caactttttt tacatccatt aatagaagta gcgttgattt ttctatctct aatgctctaa
      901 gaacggctta tattgcaact caacatatta acgatcaaag ttttacacct tcggggtctc
      961 aattcacaaa tggcttttct atatttggtt acaattttta tgcagcaggt ttgaatttaa
     1021 aaacagcacg cttattaagc gaggctatag attatataga aatagaagat tatattagac
     1081 cgattttttc taaaacaaac aatattgtaa aattaagaat tagttttgaa aaaaaagcaa
     1141 agagaatagt aggtgtacaa ttttatagtc aagaagatct atctggatta ttgacaatgt
     1201 tctctcttgc tatagaagaa caaattacat tagacaaatt gaaaatgtta gattatttat
     1261 ttcatcctag ttttagtcaa ccctataatt ttattttaaa ggcagtaaca ggattaaata
     1321 gtggagcata atcgttatta aatgaaatca agaaatatac tgtattactc aattcattga
     1381 aaatatttgt catatttttt aatagaaaac actattaaag aaaaaaaaga gaggtaaatc
     1441 aaatgaaaaa atctttaaaa cttgcatcag cagttctatt aatgtcccat ctccttatag
     1501 caacaccttt ccatgtgttg gcaagcgaaa attcaacata caaaattgca aaagaaagaa
     1561 ccaagacgac tgaaatgagt aattccgatg taacagcaga gggtagtgaa ttttcaacaa
     1621 cttcatcaag tgaaagtagt acgactttaa atgctgagtc ttcaacaact tcatcaagtg
     1681 aaagtagtat gacttcaaat gccgagtctt caacaacttc atcaagtgaa agtagtacga
     1741 tttcaagtac cgaatcttca acaacttcaa gtagtacaag tgaaataact tcttcgaaag
     1801 ttacaaagcc aaaccatata gagtcttcaa aaaaggaagc gccaaaaacg gaaggacaaa
     1861 caaaaacttt ttcgacatca cagcaaatag aatcaacaga aactttccaa tcacaaccag
     1921 tactatctgg taatgaagaa attcattttg acaaaaatga gtctgtagaa aattttatta
     1981 tgaaaattgg tgattctgcg agaaaaattg gtcagaaata tgatttatat gcttctgtta
     2041 tgattgcaca agcaatctta gaatctgcca gtggtcaaag ccaattagct caggcaccga
     2101 attacaattt atttgggata aaaggaactt ataatggaag ctttgtcata atggtaacaa
     2161 acgaagattt gggtaatgga acgctttata agactcaatc gaaatttaga gtgtatgaaa
     2221 actatgagga aagttttgaa gattatgcca aattattaac taagggaatt agtggaaata
     2281 aagattttta tgccggagca ttaaaagcta atagtaaaac atatcgagaa gcaactaaat
     2341 tccttacagg acggtatgca acagatactc aatactattt aaagctaaat ggattaatta
     2401 aaacctatga tttaactgac tatgacaaag aagttcaagg accacaaatg aataagaacg
     2461 gatatcaaat acctattcat aattattcaa tatctagttt gtttggaaat cgtggagatg
     2521 agttccatcg aggacttgat atggcagctg cacaaggtga accgattcat gcagttaaag
     2581 ctggtaaagt tattaaagca gagtttcact attcttgggg aaattacgtt gttatacaac
     2641 acgaagatgg atcaacagcg ttatatgctc accaacaaca atacttagta aaaactggag
     2701 acaacgttgt gcaaggacaa atgattggct atgtaggaag tactgggaat agtacaggac
     2761 cacacctcca tttggaaatt tgcaaagata gtagtctttc tcaaagcatg ttagttgatc
     2821 cgaaaacctt attatttggg aactaaaaag aaaaagagca ttttattaga agtatggatt
     2881 agggaatata gtagtagtgg gctctaaaga ttatgaaatc tttaagagct ctttttaaga
     2941 cgattgttta aaacgaatta accaagtagg agctttccta gaatttataa aattatagtt
     3001 tcttgtatga catgtatata gcatgctaaa ataccaataa ataaggttta agataaacgc
     3061 aaaaagtcga aaaattaaca cttattttgt gatattaatt ttaatttgca aaacaagcta
     3121 tatggaaaaa gtttaaactt tttctagagt ccctattact agttaaaaag accactgtag
     3181 tcctctgata aatcatcata aagaccacag tagtcccctt aaagtttttc caaattaact
     3241 atctgagaaa ggagagaaga tttttgaatc aagaaatagc tgatattaaa ttaatcaaag
     3301 aagttttaca agaatctacc gcaaacgcga tagcatctgg atcagggatt agcttaagta
     3361 cagcaaaaaa actaaaaagt ggcgagagaa atgtagaaaa actaaatctt tctgatgcaa
     3421 taaaaatcac tgagtttgct attaagaata ggcatgtgaa aattgaaatt tggaaatgac
     3481 ggatacaact attatagttt tgttttttct aagtatcata tttatgaata tgcttttgct
     3541 aaacatctaa ttatagatta atttttatcc aatatctata attttccaat tttttacctt
     3601 ttggagagta agggtaaatt caaagatatt gcttacttta gttgtattat taagatactt
     3661 aacaaaaatt tctccttgaa tttcttttcc tttcagttta aaagtaggat taaccaattc
     3721 aactaatttc aaattactat taattggctg aactgctttg ttttcaacat agtattcaag
     3781 ttccttttcc gatgctgatg gatacagttt aaaaaaggtc tctaaaaaat cggtgacttc
     3841 ttccgtggtt ttagcatcaa tgtcagattc gattcctatt tgttttgatt catattttgc
     3901 cttttctggc tttgcaatca tagtaggcaa actcgttact atatggctcc cattttcatc
     3961 ttcaaagatt gtcactcgat atgcagaaga aattgattgt gtcttttttc cctctgtaat
     4021 ctgttgctcg actaaaaatg aaacgataaa ttcttccgag tgtttttcaa catctagaat
     4081 ttttatagat tgaacttcgg aactcgtggg aatatctgct cgaaccatat cttggcttaa
     4141 agcgagaccg ttttctgtaa ggtactgttc gagattactc attctctttt caattgcttc
     4201 cttgttattt ctccatgaaa aatattcttt cgcaaaatcc tctgtaaaac tttcaacgcc
     4261 tgaagtattc actaccttct cttttataac tactttttca tgagtcgtat gctgatcaat
     4321 ggctgtgaaa tttctgtaga ttccaaaagc aaggctacaa gacaataaaa gccaaagagc
     4381 aagcaccatt tttcgatgct tgcccactga aatgactcgt tctttattct tttttgggct
     4441 cttttccttt tgattccgtt caatttttat cttcatgttt tttcctcttt tctcttattt
     4501 gtaccgtcca gcaccgataa gatgctgctg ccaatagctt tctgttagat ttgtccagcc
     4561 cactggatct cctgcatgaa acatgcgatt ctctcctgca taaataccga catgggtcac
     4621 ataagtaccg gtatcataag tcgaatgaaa gaaaaccaaa tcacctggtt tggcatcttt
     4681 tacatcaata tgggtcatgg cattatactg atcttgagcc acccgaggaa gtttcagacc
     4741 aactttggca taacaccatt gagtcaagcc actacaatca aacccagttt cgggacttgc
     4801 tccaccaaac acataagccg ttccttcata cttaaacgcc tcatcaaaaa tcccttggac
     4861 tgttttatca tcaaactgtg ttgtagttag gtattgcttc actaataagc aataaaacat
     4921 attgccatag ttataccgcc acccaccatt tttcttaatc gctatgggat tagggtaatc
     4981 aactttttgg ccgctcgatt tttcctttga aaaagattcg gctaaagcaa aattatgctt
     5041 cttcccgttt ttcgcgacat gatccagata cccgccacca tagttatacg cttgaataat
     5101 gctatcttga tcacacccta gttctttgga acgcttaacc aaagacgcaa aatagagaca
     5161 tccttgtttg atggatgcct cggtacttaa tgaattcggt ggcaacccgg ctgactccga
     5221 actctgcatc acatcttcca ttgtgccacc agattccact tgaataatcg ccaaaagaat
     5281 caggacatat tctgagatgt ttgattcctt ggcatatttt tccaccattg ctcggtgttt
     5341 caaaacttct tctgagacat tacttccccc agaagaagca cctgaactat tacttccttc
     5401 actgtcttcc ccaaacaaca agcccacaca catcaagacg accataaagg agagtgtcaa
     5461 gatggttaac gaaatgatac ttaatttctt tacattcatt ttttctcacc cgaccttgat
     5521 tttggacgag ggttcattct tttttgtact ttttgattgg ttcgcttggg cagagtagac
     5581 ctctgtttca cttgttggaa agaatttttc ttacttgatg gttgacttat tctttttgaa
     5641 acaatttcat gactagatag ttttcgtttg atttcgggat tcggatttac tctcacaatc
     5701 tgttttggtg cttctttttt tggcgctcta tctttttgag gttggcgctc tggttttatc
     5761 gaaacatttt cccttctttt cttatgacga ttaccggttt cgttcaatac tttgcgcttt
     5821 tcagccattt tttcatctct atgttgtcgt tgtttttctc tcaactccgc tcgatttgct
     5881 ttttcttgga caagtccgcg cttgaattct ttcggtgctt ttttcgtctt ttcaattcct
     5941 ttgtggaagt tgtatttcaa gttggtcggc gtattcctta cctgatccct tacttgttta
     6001 gtcttatctt tcaccaaagc ttgggtatcc agaactttgc ccatttttct cccagccaaa
     6061 ttcattcggg attgtttctt attatttgaa actggattct ccttagtcga gtcagatggt
     6121 acctcgtgat cgttaggtaa tcgtgaattc tttcgtagag aagaaccgga tggcttaaat
     6181 ttggattttg attgttcctt ttccaactta tgtccaactg ctgcaccacc gccaaaagcc
     6241 agagttcgga tcacatttcg attgagttgc cgcaatttac ggttcatcaa catgtgcggt
     6301 ttccgcatga tgcgccttcc cattgactga ctatcattgc tttggagatt gaacatactc
     6361 atgatgtcac ctaatttgaa atagataccg gcaaaggtca cgatttgcag gaacgcaacc
     6421 ataaagaaag gataggtagc tgaaatattg aaaaacatgg tggagatact aaaagcagtc
     6481 gtaatgacta aggtaactcc cgctcgcatc ataatcgtat tgaataaacg aataatcgcc
     6541 ttctttccca agctctcata agttggtaac atggataaca gaaaactaat tggcagaaac
     6601 atggcgaaaa tgataaagag aatttgggag aacaacataa tccccgtcag taagaagaca
     6661 aagaatgaga taccaatatt aaacaacacc aaaaagacca cggtccctag tcggttcatg
     6721 gtctttgtaa tacttagatt cgcgttgtca ttgtcttcaa tttcagcttt taccgcttct
     6781 tctcgatcct ttcccttatt ttcatctgga ctcacagata aaattttatt gacccggtct
     6841 tcaccaattt cttctatgtt ggaatcatca aattgcaaca agagccaagg ttgtcggact
     6901 tgaatagaaa acaaactatc acgaatcaaa tccacactgt cttttccttt gctttccgaa
     6961 ttcggcacaa cgagctctgt tccgagacta agagccgctt cactaacatc cgaactaaag
     7021 ttattgattt ttgtgatata ggtaggcgca tacgcgataa aggaaccaga gagaaggaag
     7081 atcgttacaa agttaatgac agcccggact gctttggtag cttctcgctt taatagtccg
     7141 atatacgcca catagactcc tagagctaaa atcattaata atagaaatcc aaaatagaaa
     7201 cccgagacct gtaatccatt ttcagtaata ccggctagcg tttggatatt tttcccaata
     7261 ctttctgccg tatccgaaat aaaatctaat ttgtacgctt cttgtaccac atacccggtc
     7321 gcatttgata aatacaaact aacggtccaa atgaaatttg taatggcgta gagaccgtac
     7381 atcacactct ttcctaaccc atcgccccag ttccaaggca gccagcccca cgaactatct
     7441 acaaagaaat ccaattgata gttatcaagt gaatattgag aatagagatt gccagattta
     7501 acggtatcat caactaatcc agttgcttcc gctactgttc caatcagact caaaagaagt
     7561 aaaatggtgc agagactaat tgcaattatt cccacaatcc gtaaaagttt ctttttcatc
     7621 tcctcactcc attctctttt gaacaggcgg gcgtgtatca aaagcatgga agagttcttc
     7681 aaagactgga tgcacttgta ccacaccaac gcgaccatac aagtcctgca tcaaacattg
     7741 cccattttcc aaatctcgca gacgcttttg attgctttca tcttcttgat caagaccaaa
     7801 gaattcgagt gtcttcttga tttcatgaat atctgtgctt cgaaaggcaa atttcagccc
     7861 aatgttgttt ttcagacgtt catctaataa atcatcacta ttttgagtga caaagtaaac
     7921 gccggcattc atagcacggc cagcacgaac tagtttattc gaaagagttt ttccttgtgc
     7981 gacttgaagg aagctccagg cttcatccaa atccacgatt ttgaaaattt ctcgattgct
     8041 atgaataaaa tccaaggcaa atgtggagat aacaatcaac atggaaacac taagcaattc
     8101 catcgtagtg tactcttcaa atgtagtctc cgcatccggc aaaaccaaat ctgccacctg
     8161 aatgatattc aattgtttct ctaaactaat cgattgtgta attgaaccat ctgaaaaaag
     8221 taaatgcgca aaatcataat cggtaaacga ttcaatatga tcagcaatat tttccgcaac
     8281 tggtgtccca tcatttcgaa gctcggtaat gacttgtaac aacccacgtt gattgctttg
     8341 ggtaacggtt cgaatagctt ttcgtaaaac gggaaatttc tctccatccc gactagaaat
     8401 tccagttaga aaagtcagaa tatcaattgc tagactttca gaatccttct ttttcttcat
     8461 gatcacatag ggatcaagta acccttgatt ttctggttta ctggttaggt tgaggatatt
     8521 aatttcactc gcaatttctg gtaaggtttc tttccagcta cc
//
LOCUS       DACEZN010000116         8490 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEZN010000116 DACEZN010000000
VERSION     DACEZN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.116"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..173)
                     /locus_tag="IXH60_002103"
     CDS             complement(<1..173)
                     /locus_tag="IXH60_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991005.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IXH60_002104"
     CDS             complement(593..1714)
                     /locus_tag="IXH60_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ1991006.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IXH60_002105"
     CDS             2042..3451
                     /locus_tag="IXH60_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1991007.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IXH60_002106"
     CDS             3448..4176
                     /locus_tag="IXH60_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1991008.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IXH60_002107"
     CDS             complement(4304..5215)
                     /locus_tag="IXH60_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991009.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IXH60_002108"
     CDS             complement(5232..6239)
                     /locus_tag="IXH60_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1991010.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IXH60_002109"
     CDS             complement(6236..8365)
                     /locus_tag="IXH60_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1991011.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IXH60_002110"
     CDS             complement(8370..8477)
                     /locus_tag="IXH60_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991012.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DACEZN010000117         8431 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEZN010000117 DACEZN010000000
VERSION     DACEZN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.117"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..774)
                     /locus_tag="IXH60_002111"
     CDS             complement(<1..774)
                     /locus_tag="IXH60_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1991013.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIH"
     gene            1004..2182
                     /locus_tag="IXH60_002112"
     CDS             1004..2182
                     /locus_tag="IXH60_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1991014.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            2198..2956
                     /gene="aroD"
                     /locus_tag="IXH60_002113"
     CDS             2198..2956
                     /gene="aroD"
                     /locus_tag="IXH60_002113"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1991015.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            2979..3464
                     /gene="ybaK"
                     /locus_tag="IXH60_002114"
     CDS             2979..3464
                     /gene="ybaK"
                     /locus_tag="IXH60_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1991016.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(3535..4788)
                     /locus_tag="IXH60_002115"
     CDS             complement(3535..4788)
                     /locus_tag="IXH60_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1991017.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            4905..6254
                     /gene="gdhA"
                     /locus_tag="IXH60_002116"
     CDS             4905..6254
                     /gene="gdhA"
                     /locus_tag="IXH60_002116"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1991018.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            6366..7712
                     /locus_tag="IXH60_002117"
     CDS             6366..7712
                     /locus_tag="IXH60_002117"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1991019.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(8020..8406)
                     /locus_tag="IXH60_002118"
     CDS             complement(8020..8406)
                     /locus_tag="IXH60_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1991020.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         2735 a         1535 c         1745 g         2416 t
ORIGIN      
        1 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
       61 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
      121 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
      181 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
      241 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
      301 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
      361 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
      421 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
      481 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
      541 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
      601 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
      661 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
      721 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
      781 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
      841 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
      901 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
      961 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     1021 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     1081 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     1141 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     1201 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     1261 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     1321 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     1381 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     1441 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     1501 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     1561 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     1621 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     1681 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     1741 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     1801 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     1861 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     1921 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     1981 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     2041 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     2101 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     2161 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     2221 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     2281 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     2341 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     2401 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     2461 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     2521 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     2581 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     2641 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     2701 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     2761 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     2821 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     2881 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     2941 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     3001 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     3061 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     3121 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     3181 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     3241 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     3301 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     3361 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     3421 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     3481 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     3541 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     3601 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     3661 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     3721 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     3781 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     3841 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     3901 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     3961 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     4021 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     4081 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     4141 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     4201 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     4261 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     4321 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     4381 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     4441 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     4501 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     4561 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     4621 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     4681 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     4741 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     4801 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     4861 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     4921 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     4981 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     5041 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     5101 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     5161 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     5221 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     5281 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     5341 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     5401 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     5461 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     5521 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     5581 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     5641 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     5701 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     5761 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     5821 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     5881 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     5941 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     6001 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     6061 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     6121 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     6181 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     6241 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     6301 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     6361 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     6421 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     6481 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     6541 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     6601 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     6661 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     6721 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     6781 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     6841 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     6901 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     6961 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     7021 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     7081 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     7141 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     7201 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     7261 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     7321 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     7381 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     7441 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     7501 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     7561 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     7621 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     7681 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     7741 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     7801 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     7861 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     7921 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     7981 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     8041 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     8101 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     8161 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     8221 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     8281 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     8341 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     8401 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DACEZN010000118         8323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEZN010000118 DACEZN010000000
VERSION     DACEZN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.118"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            20..433
                     /locus_tag="IXH60_002119"
     CDS             20..433
                     /locus_tag="IXH60_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1991021.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            446..832
                     /locus_tag="IXH60_002120"
     CDS             446..832
                     /locus_tag="IXH60_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991022.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1383..1607
                     /locus_tag="IXH60_002121"
     CDS             1383..1607
                     /locus_tag="IXH60_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991023.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1625..2053
                     /locus_tag="IXH60_002122"
     CDS             1625..2053
                     /locus_tag="IXH60_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991024.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            2031..3449
                     /gene="terL"
                     /locus_tag="IXH60_002123"
     CDS             2031..3449
                     /gene="terL"
                     /locus_tag="IXH60_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ1991025.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3461..4996
                     /locus_tag="IXH60_002124"
     CDS             3461..4996
                     /locus_tag="IXH60_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1991026.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            5076..5954
                     /locus_tag="IXH60_002125"
     CDS             5076..5954
                     /locus_tag="IXH60_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1991027.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5956..6273
                     /locus_tag="IXH60_002126"
     CDS             5956..6273
                     /locus_tag="IXH60_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991028.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6322..7023
                     /locus_tag="IXH60_002127"
     CDS             6322..7023
                     /locus_tag="IXH60_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1991029.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            7038..7928
                     /locus_tag="IXH60_002128"
     CDS             7038..7928
                     /locus_tag="IXH60_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1991030.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7951..8166
                     /locus_tag="IXH60_002129"
     CDS             7951..8166
                     /locus_tag="IXH60_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991031.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
BASE COUNT         2931 a         1276 c         1836 g         2280 t
ORIGIN      
        1 aaaggagcgg tttgacttga tgcaattgtt acgagaggta gatttcaaac agacaagatg
       61 taatgcgaga gatgtgctga agaactttcg gcgtttggag cggatggcag gtcgctcttt
      121 gatagatatt aagtcgccga ttattacgga tatgccgaag gcaccgaagc acggcaataa
      181 ggcagaagac gcgatcattc aaatgatgga tatagaagcg gagagagacg cgattctagt
      241 ggctttgatg gctcttagtc tgattagtcg tcagatactc tactacagct tctgtgtgcc
      301 agatagcttc tcaaactaca ggattagccg tgaagtgggt tattcagaaa gaagcataca
      361 aaggatgaag tcggaagctc taatagagtt tgcagaagcg tataaacacg gaagaataat
      421 tgcttataaa tagggggacc atataatgtg gaaagattat gtatcgttga aagagttgaa
      481 aaaagatctt gttttcaaaa gaattgtaga atggtcagag agtgaattaa ttttagaaga
      541 tggaaccaaa atggaagttg tatgtagcga atcagattgc tgcgcatggg ccgaaggtga
      601 attcaaaaat gtaaagcttg atgcagtgat tacagatata aagatttttg ataaaggtaa
      661 ccgtctctat aatggtgacg gacatacctc ttatgctgaa gtcgttgttt atcataatcg
      721 taatgagatc gctaaagcag aatgcacagc gaatgatggg aatggcggct actattatag
      781 tgtttgtgct ttaaaggtca aagacaaact ttgtatagtg actgacgcat aaaaaaatgg
      841 cggttttttg gcggaatgat ggcggttttt agccatttat cagtgatatt atggtagtgt
      901 cgaaagatta gtgataggtc tgagacaaaa taataataaa aggaacatcg ttttattatt
      961 gtttcacaat taagcttcga tagacagcaa cggaaatatt aagaataagg atgtgaattt
     1021 taactccttc taaattgttc ttattatcta tcatccgttg ctgtctattg ttacatacag
     1081 atcgcttagg cggtcttttt attttggccg aaaacctaca ttttcgatgg ccgattattg
     1141 gaggaggaat agcatgttca aactatctga aatcattaag aagtccgacg ttgagaaatt
     1201 agagatgtta aaaaagaagt tgaagaaata gcagtctctt cgtgaggctg ttttattttg
     1261 ctcacaaaaa tagaccacta tcgggtaata gtggtcagga attaaatgaa aaagatgtta
     1321 gggttgttag ctaagtataa catcataacg cttacaaaac aatgcaagaa ggataaatta
     1381 ttatgagaac ttactggtat gtgtcattaa acaataaata tccgaagcca atgaaaggac
     1441 agcataggcg tgtagtcatg tctgttcaaa tgaaggcgaa gtattcgatt gtagaaatga
     1501 tcagagaggc aacgccagta gagattgatt attgcaagct agtctattgt gggtgcggcc
     1561 gttggaaaga ggatcatgta caaaaaaata tcagcaagta catatagaga ggcggtgatt
     1621 aaggatggaa aaaagcgata cgaaagcgaa cagatacaaa cctactgctg cagagaaaaa
     1681 actcttggaa gtattgataa atccagataa cttaggcaaa ccggtgcaag agttgtgttc
     1741 actggcgaaa ataggtagga ccaaatatta cgacgccatg agcaaagatg gctttgttga
     1801 tctagttaac gaaacaacaa tggatctgat caaaggaaaa gcttctgatg tccttaatgc
     1861 cacttacaag tatgcattga ctgaaaaagg tcatcaggat aggaaaatga ttctgactat
     1921 tgctggaatc tatgcagaca aacaagaact caaacattct ggcggcgtcg atatccgaaa
     1981 acagtatgag aaaatgtccg atgaagaatt agaagagttg gtgaaacggt atgaaaaaat
     2041 caacgactct taatcgtgtt gagtatgccg ttgttcaaag agagctagcc attcgaaaag
     2101 ctaggaaaag tttttggcta ttctgccagc ttctttatcc agagttttac aaagatgatc
     2161 ggttgtattt gaaagattta tgtcatacgc tacaagattt ttacgaagat aaaatcgaca
     2221 aaaaaatttt ggttattaac atgccgcctc gacatgggaa gacttttaca gcgcggctat
     2281 tcgttttatg gatgtttgga caaaatccaa agacgaaaat tattaccgga tcttataacc
     2341 aaatcctgtc atctttattt gcgcaacaaa cacgtgatgg aatcatggtt gaaaacgaaa
     2401 atgttaaggc tgaatacttt aaagatattt ttccagaggt gcaaatcaaa tatggagatg
     2461 ctgcaaaggg tttctggtct ttagatggtt cagaagaaaa gaattattta gcgacttctc
     2521 ctggtggaac atccactggt attggcgcga attatgtaat tgttgatgac atcattaaaa
     2581 ccgcagaaga agcctataac gagcgtgttt tagattctca ctgggaatgg tataacaaca
     2641 cgttggccca acgaatggaa cggccacgta aacagatatt gattatgaca cgttggtcaa
     2701 gtaatgattt agccggaaaa atgttgacga gacgaaaaaa taatgttcat cagatctgtt
     2761 ataaagcagt acaagaagac ggctcgatgt tgtgcaacga gattatgacc cacgatgagt
     2821 acttagatgt ggtacaagaa atgaatgtag atattgcaga agccaactat cagcaagaac
     2881 caattgacca aaaaggcagg ctatatcaga agtttcttac ttatgatacg ttgcctgata
     2941 acattatcaa aatatggaac tacaccgaca ccgccgacaa gggtgccgat tactttgcgt
     3001 ctcctgtttt tgctgagacg tctgatcatg atgcttatat tatcgatgtc atgtatacaa
     3061 aagagccgat ggaaacaact gaaatagccc acgctaatat gattattcgc aatcgagtta
     3121 ataaggtacg gattgaagga aacaatggcg ggcgtggatt cagaagaaat tcagaaagaa
     3181 ttacaaaaga gcgaggctat cgaggtgcag tatttgaaga ctttcatcaa tcggataata
     3241 aactgtctag aatactggcg aatagtgctt gggtggaaaa tcatgtttac tatccagatg
     3301 attgggcgac acgttggccg gacttttatg aggctatggt tagttatcaa cgtgaaggta
     3361 agaacgctca tgatgatgct ccggatgcag ttacaggaat tgcagaaacg attatagaag
     3421 cagatgactt cgagtttatt gcttattagg aggtgggtct ttgttttatt ctggaatcac
     3481 attgaaaaaa tacaaacgtt taagaacgaa atattcaacg caaatcgaag aaggattatt
     3541 cgatcctaac gcttttatgg aagatatgaa accctttttc gatgatcgag aaagaaagta
     3601 tcttgcttat actagcgaga aaaacgagat cgacagtcgt gagaaaccaa aaacaaaaat
     3661 cattaaggtc aataacaaga ttcatgcggg aatgtattca gtggtcgttg atcaagcagt
     3721 taaccatttc acaggaatac caattaaatg ggattatgat gtttcggaac agaaaaggac
     3781 attcattcaa cgaatgagag acaaattact gggtaatgat atcagcgccc cttctacccc
     3841 agaagaattt gaaacactca cgagacaatt aagtactatg cggtttggaa tgttagaccc
     3901 cgatactgca acatttcaag gagcgacagg gcttgctttc agattactgg agcctgttga
     3961 gacaaatgat ggttgggaac taagagcaag caacattgag ccgtggaaag ctgaaaagta
     4021 cgaaaacggc gcagtgtaca ttaaagagaa atacgatcca tatcaaaaga agttttatga
     4081 agagatgaag gttgtcacgc cgaaatcaat atttacttat gctcgatata ctgattaccc
     4141 tggcacggta caaggagcat ttaaaacgct gggtaatgtt cctaatcctt taggcgttat
     4201 caatcttacg gagtttaaaa acaatacaaa ccgttactgt gactttgaag ttgcagagga
     4261 gattagtgat gctattgatc gtgttttatc tgatcagcag aacgagattg aacaattcaa
     4321 gttagcttat atgctgatca ctggggcaga tttgtccgag gataaagcca agaagatgat
     4381 ggagcaacta gggatcatca atctgaaaga ccctaatgcg aaagccggct acgtgacaaa
     4441 ggatctacaa aaggatttca acgagtatca ttttgatctt cttaaaaagc aattctacac
     4501 ggtgtgtaaa gcaattgatt tcaatgacga agtctttaag tcgaatagtt ctggggaagc
     4561 ccgaaagtgg caaatcatta gtttagaagc taagacaaat accaaagaac agtactttcg
     4621 agaaggattg aaagagtgtg ccgaggttat ggctgcttat ttggattttg ccgagaaagt
     4681 tacaattgat ccggaaaaga tcattttcac cttctctcgt tcgcttccaa cagatttaag
     4741 ctacctagct gaggcattgc ctaagttggc accatacgtt tctaagagga cgattcaaag
     4801 tcagatcccg ttcgtcacag atgttgatta tgaaaacgaa atgattgacg tcgaaagcag
     4861 acaatcttac ccagacggcg agtatggagg tggcatcgtt gagtcaaata gaaacgaagt
     4921 actggaccaa acgtcgagaa ctggaagatt tagcaagact caaaatggaa aatcaaacgc
     4981 taaagaaact aagtagtgtc tttcctgatg cgttgaaaga aattcaagcg gagttattat
     5041 cccaagctga tttacacgat attacctatg ctgagatgat ggaattttat agtacaagtg
     5101 atcagaaaaa atatcgtgaa tacgttgaaa agaactacaa atcactgaag atgtatgatg
     5161 caaaatacaa agagttcatc gaagaatact ttccttcttt cgactacgcc aaagtgaatc
     5221 ggctgcttca aatgaggtca gatatttttc gaattcttgc agagagtgca atagatgcag
     5281 atgtgaataa atatttttcg gatcggttag aagatatctt gcaacgttcg tatgtatcaa
     5341 acgcaaacgt atttgcccag attttgaacg tagatttgcc gaattactta tcaaaggaag
     5401 aacttgaacg ttacctgaac tatccttggt caggtaagac attctcgcgt cgtttatggg
     5461 gcaatatttc atctctagaa caaaagctat ctaacgccat tacaactagt gtagcaagtg
     5521 gcgaaggagt tctcgtggca ttacgaacaa tgcgagaaga cccagaaata tgcgatatgt
     5581 tcaaaaggga ggaatcgaag tacaacaagg cgatcgaaaa tcttgtgaga acagaatacg
     5641 ctaaattcgc tcaagatggt attgagaaat cttatattga aactggcgta gacgaataca
     5701 atgtcttaac tgcaaaagat gaaaaggtat gttcaatatg cggtggcaaa gcaaagaaca
     5761 acccatacaa gttgagtgaa gcgatcattg gtgagaaccg tgcgcctttt catggccgat
     5821 gcagatgtac agatgttcct aatatgccga aattaggaaa agatattgac gaagagtatg
     5881 agcgtttatt tggcgattta ttagatgagt ttgcacatga ttcttttgga attaatttga
     5941 aacggaggaa gtagaatgaa agattttttt gaagcagtac taacaattaa tgtaaatgct
     6001 gatattgcag aagcctacaa aacagctatt gagtctgaga accatcctaa tggcttgaga
     6061 gaccattgga atggcaatta tgcctacgtg gttattggcg atcaaactgt taattatcaa
     6121 gataatactc cagttgataa gaataccgtt aatttaacga ttcaattatt atctcattca
     6181 ttaccaaatt taaaagaaac agttgattgg tatgaaaata tgggatgtat tgttgttaga
     6241 actgactaca aagaaggaaa gtctagtaat taggcttttt tattttgtcc gaaatgacgt
     6301 taaactagcg caatactggg cttggttgaa tggtggggcg caactattaa aactcaaagc
     6361 aatgcggggc gtgaaaacga atcgtggggc gaaaggagaa tgattatgaa acacaaatca
     6421 ttaatgccaa tgaatttgca atactttgcc gaaggtgacg atcaaaagtt ttcttttgat
     6481 gacttcaaat cttttgtgga atccaatgaa gaagctcaaa aatttattca gtcacaatct
     6541 caaagtgttg ccgacaaaca attggaagct tggaaacaaa ataacttaga taaaattaaa
     6601 caggatacca tcaaggaata tgaggaatct aagaaaaaca agtcacctga gcaaattcaa
     6661 ctggaaaaac tacaagctga atttgaagca gaaaaagcgt tgcgtgtgac aagtgataat
     6721 aaagcatttg ttgcagaaca aattgctgga ttagaactag atggagagtt aaaagagtct
     6781 atttctcaat ttatgctaaa taatcttgtt agttcggata cagatttcac taagaatgct
     6841 gttgaaggtt ttacaagtgt tttgaatgca attaatgaga aacatgcaga tgcattaaaa
     6901 gaactacaaa tgaagtctgc atttggtgga actcaacaat cgaataacca ggttcagcag
     6961 aacaatgaaa cattaacaaa tccagaagaa caattaggac aaattcttca acaatttaac
     7021 taggagagtg aaaaattatg aaaaaaacat ctttaaataa tttagagtat ttggatattt
     7081 caccagcgat taatgctatg caagtaccaa atacaccttt tttaagctat ttacttggtg
     7141 ctggaaaaac agagccagca aactcgacag aaattaaatg gcgtgaatat gatatcaaca
     7201 acgatgattc ttctgaaaaa cttgagggcg gagaatatcc agatgctgaa tcaggtcgaa
     7261 cttggtttaa caactatact gaaattttta gaaaatcaac ctctgtatct ggtacattag
     7321 atgctattaa tgtgaatgga gtcggaaatg aattaactaa tcaagtagca ctacgtggta
     7381 tggaaatgaa aattgatttg aaccgaaaat tgattactgg tgtaaaagct gatgaaaatg
     7441 gttctaaagg tcgtcgaatg aatgggattt tgaacttgat caattcagca aataaggcag
     7501 aaacagccac tgcgggtgca gtaacaagaa aagatatcga tactttattt aaattgatgt
     7561 atgaaaaagg ttatatggga gaaaaactat gcttgatttc tccagatatg caggagttaa
     7621 tgactgatga gttagatgga aaatcaacaa aaattgttca gttcggggaa agagtaactt
     7681 ttggattgca aattggaaat atcgtgtcta attacggtac aggtattgct ctactagaac
     7741 catcattgcc aaaaggaaca attgccgcaa tcgatactaa ttatgtgaaa ctacgtccat
     7801 tacgtgaatg gagagcagaa gaacttgcaa aaacaacgga ttctagacgt atcggtcttg
     7861 taggtgaata ctctcttgaa tacaatgctt caaactctgg ggcaatttta aatttaaagt
     7921 ctgaataaaa gggagttagt actccctttt ttgataggag gaattacagt ggtaaaaaaa
     7981 gatgaaacta aaaaagatga agtcgtgaaa tatagagtag gtaaaactaa aaattttgtt
     8041 gggtttgttc atcctaaaac tcgtagattt atcacagcag attcaaataa cgaatttatc
     8101 atttctatag atgataaaga agcaattgca attttggaag atgcaattga tgttaatgaa
     8161 atttaggaag tgatctgatg gatgaatcgc taaaaacgga aatcattgag tctacaaaag
     8221 aagattttcc agatttgagt gaagaacgca taactaattt attagaaata attttgctag
     8281 aaattgaatc atacaacact tgtaaaaatg atatttcatg gga
//
LOCUS       DACEZN010000119         8323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEZN010000119 DACEZN010000000
VERSION     DACEZN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.119"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            166..600
                     /locus_tag="IXH60_002130"
     CDS             166..600
                     /locus_tag="IXH60_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991032.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(678..1211)
                     /locus_tag="IXH60_002131"
     CDS             complement(678..1211)
                     /locus_tag="IXH60_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1991033.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1305..2483)
                     /gene="mutY"
                     /locus_tag="IXH60_002132"
     CDS             complement(1305..2483)
                     /gene="mutY"
                     /locus_tag="IXH60_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1991034.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2476..3273)
                     /gene="recX"
                     /locus_tag="IXH60_002133"
     CDS             complement(2476..3273)
                     /gene="recX"
                     /locus_tag="IXH60_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1991035.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3380..4762
                     /gene="rlmD"
                     /locus_tag="IXH60_002134"
     CDS             3380..4762
                     /gene="rlmD"
                     /locus_tag="IXH60_002134"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1991036.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4936..5514)
                     /locus_tag="IXH60_002135"
     CDS             complement(4936..5514)
                     /locus_tag="IXH60_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1991037.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5836..6306
                     /locus_tag="IXH60_002136"
     CDS             5836..6306
                     /locus_tag="IXH60_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1991038.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6414..7640)
                     /locus_tag="IXH60_002137"
     CDS             complement(6414..7640)
                     /locus_tag="IXH60_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1991039.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2443 a         1645 c         1407 g         2828 t
ORIGIN      
        1 agactgtcta agtttttcaa acaatagatt tcctcctttc tttcttacta gtatatatta
       61 tattcgttaa aaattctttt attctttttt tatttttgaa aacttgccat actttttatt
      121 tgttttattt tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa
      181 acaaaagata caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt
      241 gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc
      301 cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta
      361 gaaaacggaa aaatgaaatt acagcggatg gcgattcttt ctgaatacag acaccaaggg
      421 ttaggaaaaa tattgattga agaagcagaa aactttgcta agaatcaagg ctataatacg
      481 attttattag gggcacagtc cacagctgaa actttttacg aaaaactagg atatacagct
      541 tacggcgatc cttttgaaga tgctggcatg cctcatattt atatgaaaaa gactttataa
      601 aaatttattt cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc tcactggctt
      661 actgttcttt tttgtgttta cttacgcgac aattgcttgt aacggttata ccaaatatca
      721 atatagcctt ccgagaacgg gccatctcca ttattgatcc agtcaaccag aatcttcaca
      781 ttttctttta ggatgaagtc aatatcgttc gggtaattca tcattttgct gtgcatctca
      841 tattcatcga catccaatag tcgtttctcg ccgtcaggaa ataccttgat atccaagtca
      901 tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag attacaataa
      961 taggaaaccc ccttttctct gatcattgct attacattga accagtattt tttatgaaaa
     1021 taaacaatcg ctggttcgcg cgttacccat cggcgtccat cagattcagt gactaatgtg
     1081 tgatcattca cgccgatcat agagtgttcg cttgttttta ataccatggt atctcgccac
     1141 gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc tccttctttt
     1201 ggaactccca tagcaaccct tccttaagca gcattttgtg ctttgtgttt gggtgttcat
     1261 tacaccgaca tcataattat tatatcaata aaaatgagct ttgcctagaa aggattccta
     1321 ttgtttcgat ttttctctaa ctgatctacc agtttcattt ggaccttagg aaaaacgacc
     1381 gaatcaaatg catcaggctt caaccacttc gaagttttgt tctcggtaaa ttcttgttct
     1441 gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg actaaaaata
     1501 tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg
     1561 agattttgct cttcttctgc tactaggtca tcaaacagat cgatttctgg ctttgtttcc
     1621 cattcttttt tcaatcgctc atactcctct tgtgtcactt ccatcattgg aaatgtccac
     1681 atattcgcca gcaatttttg gctatctctt tcttcaaaat aatacgctcc agaatgattt
     1741 tgaagagctg cactgatata ataaacgtct tttggctttg ctttttttgt ttttacagga
     1801 aaactcgttt ggattcctcg tttgttagct aaacaaaagg cttggatcgg acaagtctca
     1861 cactttggag aagttggtgt acagatagct gaacctagat ccatcattgc ttggttgaat
     1921 tcacctggat gcttttcatc aatgattttc cgcatcgctt catcaaagat tttcctgcta
     1981 gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg catcacgttg
     2041 ccgtcaactg caggttctgg aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat
     2101 ggtcctattc ccttcaatga gctaatttct tcaggcgttt gcggcatttc cccatcaaat
     2161 tctgacatga tctgtttagc agcagcttgg atgttccggg ctcttgagta ataaccaagg
     2221 ccctcccatg cttttaaaag cttttcttct ggagcatttg ctagctcttc aattgtagga
     2281 aaccattcca tgaaacgata aaaataatcg atcactgtgt ctactctggt ttgctgaagc
     2341 atgatctcag agatccagat acgataagga tcacgattat agcgccaagg caggtttctt
     2401 ttttcttgtt cataccattg aatgaactga tcttgaaatt cttttgtttc tttatctgtc
     2461 catttttccc attctttatt cactttcctc ttccttttcc gtaatgaatc gctggatcat
     2521 atccaaatca aatccttttt gatataagct ttgctttact tttagatttc tttttttagg
     2581 ttcaaacctt aaatttcttc gccatagttt atctccttgt ttgactaaat aatcatattc
     2641 tatttcttga tctgcttctt gcggaagatc agctatcgct tgttggatca cttcttgaga
     2701 aaagcctttc gtcatcaatg tttgttggat ttttctcaat aattcttttt ggctttttcc
     2761 gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa
     2821 tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt gctgcatctg
     2881 ctgcgctaat ttctttggtc ctttgtcact taagcgcata ttcgtgcgca cataactttc
     2941 cccatagacc aaatcatcga caaggtccaa ttcttttaag cgggctacga ttttatttcg
     3001 atcttcttga aggatttcct tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa
     3061 ctgataactt aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt
     3121 gatctcttgg atagtttctt cgtctaattc ccgtcctttt aataatcgat agcgtactaa
     3181 tacatcttca gatacttgta aagcttctcc ttcagataaa tctacgcgat agaaagggcc
     3241 tttgccttta ctgattctcg taacggttaa cataaacatc ctcctcgaac atatattctc
     3301 tatcatacca cagaaaatcc ccgtaatcag gtattatatg ttagaataaa ccacagaaaa
     3361 aagaaaaagg gagatactta tggcagaaac aattgtcaaa actggacaaa cggttccttt
     3421 gaaaatcaag cgtttaggca taaatggtga aggaatcggt tatttcaaac ggttgatcat
     3481 atttgttcca tatgcactac caaaagaaga agtactagtg aaaatcacga aagcaactcc
     3541 tcgctatgca gaaggagaat taataaaagt caaaaaaaca agcaaagatc gcgtagtggc
     3601 accatgtcct gtttactatg aatgcggtgg ctgtcaatta caacacttag cttatcaagc
     3661 tcaattggat tttaaaaagg atctgctttt gcaagcattg gaaaaattca aaccagcagg
     3721 ctttcgatct tatcaacttt tgccgacgat cgggatggat gagccttggc attatagaaa
     3781 caaagcacaa tttcagttac gaaaaaataa gcagactcaa aaaattgaag ctggtttata
     3841 cgaagcaaac tctcatcagt tagtccctat aacagactgc ttggttcaag aacctcgaac
     3901 acaaaaagta atgaatactg ttgttcaact attgaataaa tttgatgcac ccatctatga
     3961 tgaacggaca aacagtggaa tcttccgcac aatcatggtt agaatcggcg ttaaaactgg
     4021 ggagttacaa gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat
     4081 ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca
     4141 aaaaacgtct ctcgtgatgg gggatgaaac gatccactta tggggaaaag aagccattga
     4201 agaacaaatc aatgaagtga ctttcgatct ttctcctcgt gccttctttc aactcaatcc
     4261 tcagcagacc gaattgcttt atggcgaagg aatcaaagca ttagatgtcc agcctaatga
     4321 aacgatcgtg gatgcttatt gcggagttgg gacgatcggc ttaagtgtag caaaacaagc
     4381 caaagaagta cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc
     4441 ggaaagatta ggatatcaca acactcgata tgaagtcggg acagcagagc aagttctgcc
     4501 taaatggttg aatgaaggtt ttcagcctga tggtatcatc gtagatccac cacgtacggg
     4561 tttggataac gcattgatcc aatcattatt gaaataccct ccaaaaaaac ttgtttatat
     4621 ctcctgtaat gtttctacac ttgccagaga tctggttaat ttaagtaaaa agttcaagat
     4681 tgaatattta cagtccgtcg acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa
     4741 gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt
     4801 aataagaaca gccaaacaaa aatagctctt catgttttat gaatactgta cttgtgaccg
     4861 aagagttaac aggaggacac tatttattta aaaaaagggt tgtgacatag tttttgtcac
     4921 agcccttttt gattattatt ggatattcac gtctatcact tgataaaatg catttgtcgt
     4981 atcgctgata ttccatactg ctaaaatcac attatatcct tttcgatcct gcggaagtgt
     5041 gatcgtttgg ttgactgttt ttggtggaac agttgctttg tcgtctattt ttgtgatcaa
     5101 ttcaaaatca ctaaatttca aaggttgatt aggattccaa gttggtttgg taatgtagta
     5161 atcccaagta cttgtccgat gttgtgctgt taaattccaa gtaatattta atgttcctgg
     5221 attaaccatg tttttatgcc atctgttaga tgtctgttca tctaaaggct caaagccgct
     5281 gactcctgca ctagcaattt ttccatcaat gaatgtattt ttggtagctt cgatactttg
     5341 cggttcccat tgtgctcttc cgacattttg atttaagttt cctgctgctg atgttcctag
     5401 gtatgcacgg cttcctggag aagtaatata accatgcgca aaacctggaa tgctaaaacc
     5461 gaacaaccct aaactaacta acccaataaa caataacgta ctaatctttt tcatgtagaa
     5521 acctccttca tttgataacc acattgtaaa cgctttattg tattaacaca aacattatgc
     5581 ttttataatt ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga
     5641 agtagacgag aaaagtacaa atagaaaaca tgagaatcaa atcatatgat ctttttcaaa
     5701 aacatcattc atataaacaa atgttcaact ttttaataac aacaattatc aatttttttc
     5761 ttacttcaca aactaaatac aggtcttgct gtgagccttc atggtaaact aatattatag
     5821 aaaggagtta gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg tgttatagga
     5881 attgtcatat taggcttttt atcagggttg cttagcggaa atcccggaaa atattactat
     5941 tctttgcaac ttccaccatt tgcaccacct agctggatat ttgggccgat gtggacctta
     6001 ctgtatatat tgatgggtat ttctctttat ttgttactta atcacaacaa caaaaaacag
     6061 cgaaacaatc ttgtcggtct ttttgtgatt cagttcattt ttaattttat atggtctgct
     6121 ctatttttta atttaagaaa tattttcatt gcagcaatcg atattacact tttagttatt
     6181 ttcttgtcag tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat gtggctcatg
     6241 attccttatt atttatgggt actattcgca actctattga attattcaat ttatttttta
     6301 aactaagcgc taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt
     6361 tagatttttt gtctaattta ggtggcactc tttattaacc aaactatttt tttctagtac
     6421 ccacttgttg ttccatactc attattctgg tcataaccat aaccattttg ttcctgtcca
     6481 gcgtaaggat cataactttg agtatcttga cttggtgtat agtactgact tgtatcttct
     6541 gtttgctgat aataggtatt cggatcttgg tactgatcat atgtgttttc tgaataggaa
     6601 gaataatcgg tgtacccttc actattcgat ccatcaccgt ttgtcgaatc gccatcttcc
     6661 gaatcatcaa agaacatcat tgaagcattc tcatttttca aattaggtaa tgtatcgctt
     6721 gtttttatat ttaattgttt cttcagttca ttttggatcg aaagaagatc attttttcct
     6781 aaaatttgat agtagatatc attgatcatt tgtccttcgc cttgcaactg gtcttgtttg
     6841 attgtttcaa acgccggggt gtagttcgtc gctacatcaa gcatgtcatc ccaatctaag
     6901 tccgtcgtca cattgttctc aactgctttt aaaatttttc tataattgct tacgccatct
     6961 aaacttaaaa ctttattgac gattttcgtc actacttccc gctgacgacg ttgtcgaccg
     7021 atatcgcctt ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt ttctccattc
     7081 aatgtttgtt cacctactgg tacatggaca ccatccaaag taaagtcaat tttattattc
     7141 actttgatgc caccgaccgc attgatcaaa tcttccatgc catccaaatt gatcgttaca
     7201 taatgatcaa taggaatatc gagcagttgc tcaattgaat ccatggccat ttcgacacca
     7261 ccaaatgcgt aggcatgatt taatttatct accgttccat aacctacgat attcgtataa
     7321 atatctcggt ccaaactgat gatcgttgat tttttttgtt tcggattaac tgttactacc
     7381 atcatcgtat cagaacgtcc ttgttccgtt cttcctaatc caccggtatc taaacctaat
     7441 agcaaaaccg aaaaaggttc tttgtcatca atgcttactc tctcagaacg tttggaggat
     7501 attcgattag agtttttaga aattttgttt actgtttggc tcgcttctcc cataaatctg
     7561 attccgtatg ctgataatcc ggcaatcagc aaaacgaata cccctaacaa ggttaataga
     7621 actttttgcc atcgcttcat aatacacctc tgtttcacat tttctaaaat attgtctcat
     7681 ataactgttc aatcagcaaa ttcacttttt aatcataaga aaactttaaa aaacattttt
     7741 gtgtaacgat tattattttt taaaatttct caaaattctt aactatcata ccataccgtg
     7801 aaaaagatcg aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt tttatgtatt
     7861 ttttccagaa aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta aaatgatcgc
     7921 acataccttt cccatctttt taacagtctt tgcctaccgt tcagattatc attcgatttc
     7981 acgacaattt cttcctatga ctggtccttt tgttaaaccc atcttctatt cgttttattt
     8041 ccagctcact gcatggctct taaaatgact cgaactagtg attgcattac catcatcttt
     8101 taatggatag atatgctcct cttcaaagta aaacgatttt cttttaaagt aatagaattg
     8161 acatggtaat gagtatgtat actagtggtt tcacaaatgg tcttttattc aacatccaaa
     8221 aactgaaatt tctttaaaat ccattactaa cgaagaaaat gttccttgct tgataattag
     8281 ttaggacatg tcaagtgaac aaatgcattt acttagccaa aaa
//
LOCUS       DACEZN010000120         8303 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEZN010000120 DACEZN010000000
VERSION     DACEZN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.120"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            47..679
                     /locus_tag="IXH60_002138"
     CDS             47..679
                     /locus_tag="IXH60_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1991040.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(681..1586)
                     /locus_tag="IXH60_002139"
     CDS             complement(681..1586)
                     /locus_tag="IXH60_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1991041.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1671..2324)
                     /locus_tag="IXH60_002140"
     CDS             complement(1671..2324)
                     /locus_tag="IXH60_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1991042.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            2646..3590
                     /gene="mvk"
                     /locus_tag="IXH60_002141"
     CDS             2646..3590
                     /gene="mvk"
                     /locus_tag="IXH60_002141"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1991043.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3583..4560
                     /gene="mvaD"
                     /locus_tag="IXH60_002142"
     CDS             3583..4560
                     /gene="mvaD"
                     /locus_tag="IXH60_002142"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1991044.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4573..5658
                     /locus_tag="IXH60_002143"
     CDS             4573..5658
                     /locus_tag="IXH60_002143"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1991045.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5655..6719
                     /locus_tag="IXH60_002144"
     CDS             5655..6719
                     /locus_tag="IXH60_002144"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1991046.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6968..7771)
                     /locus_tag="IXH60_002145"
     CDS             complement(6968..7771)
                     /locus_tag="IXH60_002145"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1991047.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="IXH60_002146"
                     /pseudo
     CDS             complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="IXH60_002146"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(8078..>8303)
                     /locus_tag="IXH60_002147"
     CDS             complement(8078..>8303)
                     /locus_tag="IXH60_002147"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1991048.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         2509 a         1741 c         1625 g         2428 t
ORIGIN      
        1 gattcccgct tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga
       61 acctattcca ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga
      121 gatcttctct ggtcttctga tcaacggagg taaccaaagc tatctcgatg ccaaaaatat
      181 ggtattaaga gagagtcata tccaaaaact tacgatgcaa aaaacaggtt tacgtcattt
      241 tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg
      301 cagtttccac caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag
      361 ttatcttcga gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac
      421 aaacttgaaa gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt
      481 cacttggaaa aacctttttt tgtctgacaa tcagttaacc ggttcgaact ggtttcgaac
      541 ttctctgaag ggattagact ttacgactaa tttcttcaca acaatcgctt tatctcaaga
      601 ttctttggcg ggactaatcg tcaatcaaga gcaagcactt gtgatcgccc aagcccttgg
      661 tttgcagatc aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata
      721 gataagacca aactgatgat caaggcgatt tcccatccga aaacggaatg ggtaaagggt
      781 aactggagat tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac
      841 cagaccgtca aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg
      901 ccagagagct ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct
      961 tcgatcagtg catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc
     1021 agtgccttga tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc
     1081 aaaccgatct ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat
     1141 ttcagattaa gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca
     1201 gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac tcatgtctgg catccgttcc
     1261 aaaagcgatc gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa
     1321 ccgtcttctt ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc
     1381 tggtgcggaa catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc
     1441 gcccgttcat ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct
     1501 tttaagtttt taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct
     1561 tgattaaaat agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa
     1621 gaaaaaagtc gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggat
     1681 actttggttt ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt
     1741 gcttgaggat cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct
     1801 ttgtattctt cataccattg atcataacgt ctgttcaatt ctttgtaata atcgtataga
     1861 cttgggtcaa aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctctaacatc
     1921 gtatcaaaag atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag
     1981 ggaagttctt ccatcatgtt ttccagcagt tcatcataca cacaaacttc tgtttctgtt
     2041 gctcttccta gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc
     2101 aatacattat tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa
     2161 taaatttgca gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca
     2221 ttatcatcaa tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt
     2281 aaacttgatt ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag
     2341 ctcacttcat cactaaatat tgtgtttaac tccaaccttt tttaaatata cccccaatat
     2401 ctagtagtgt caattgtatt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa
     2461 gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag
     2521 taaaataaag gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc
     2581 gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt
     2641 agattatggc aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc
     2701 acgcggttgt ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg
     2761 cattccttga agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa
     2821 atttagccga agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa
     2881 aacagcatga catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac
     2941 gtggaatggg atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact
     3001 tagcatttcc tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg
     3061 cccacggtaa tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt
     3121 ttacaaaagg gcatcctttt gactactttt ctttgaacat cgatgctttt ttgattgtcg
     3181 ctgatacagg aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg
     3241 aaacccagcc tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag
     3301 caaaacaagc aatcattgag aattcaccag aaacgttggc acagacaatg gatgaatcac
     3361 agtcacttct ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag
     3421 caaaagatac cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga
     3481 ttgcattagc acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg
     3541 gtgctgctga aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca
     3601 cgcgcatata ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc
     3661 cttccaatga acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc
     3721 atcttttctg atagctatat ggtagatgaa ttttatctag atggcacctt acaagacgaa
     3781 aaagcaacaa aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca
     3841 ctaaaagctt cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct
     3901 gccagcgggc tagctgcttt agcaggagct tgcaatactg ctcttaagct tggattagac
     3961 gatctctctc tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc
     4021 ggtggtttcg ttgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc
     4081 ccttccgatt ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa
     4141 aaagaagtgt ccagcagaaa tgggatgcgt cgaacagtcg aaacatccag tttttatcaa
     4201 ggctggttag attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa
     4261 gatttccaac ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact
     4321 ttagctgccc agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg
     4381 gtgcggcaat tgagaaaaaa aggcattcca tgctacttta cgatggatgc tggccctaat
     4441 gtcaaagttt tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta
     4501 tttagcaaag aacaagtaat tactgcccat gctggtccag gaattgcgat tattgaataa
     4561 agggagattt ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat
     4621 gcagttgttg aaacaggaca tccagcagtt atcgctgcag tcgatcaatt cgtaacagtc
     4681 actgtagaat ccgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct
     4741 gtacgttgga caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat
     4801 tatatccttg ctgctattcg tttgactgaa aagtatgcac aagaaaaaaa cattctttta
     4861 tccttttatg atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga
     4921 ttgggttcta gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg
     4981 ttgaatttat ctcaattgga gattttcaag attgcagcac tagccaattt agcagttcaa
     5041 gataatggtt cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca
     5101 accttcgacc atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt
     5161 gccctggatt ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta
     5221 ttgattggtt ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt
     5281 tcgagagaag acaaaatggt ggcttatcag cttttcttaa aaaacagtac agaatgtgtc
     5341 aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga
     5401 aaaaaccgac aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct
     5461 gctttgaaca aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt
     5521 gcaggtggtg gcgattgcgg aatcgtaatt gttgaccaga aatctggcat ccttccttta
     5581 atgagtgcat gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa
     5641 agaaaggaaa accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg
     5701 ataaacaaaa aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag
     5761 cagtttctga tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt
     5821 atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa
     5881 tcgtcgcaag agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag
     5941 acccttcttt agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca
     6001 ttgccaacat cggtgcaggg acttctgtcg aaagagccca agaagctatc cgattgtttc
     6061 acgcagatgc cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag
     6121 accgtgattt taccaattgg aaagtactta ttcaagagac tcaaacagcc attgacgtcc
     6181 ccctcatcgt aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg
     6241 ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg
     6301 aaaacgcccg tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag
     6361 tctcttcatt gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag
     6421 gtatccgcaa tgcctatgac atatttaaag ccctttgtct cggcgcaaat gcagtgggta
     6481 cttcaggaac cgtactgacg catctgatga atcacggcgt agaagaaact attatactga
     6541 tgaagcaatg gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg
     6601 cggccctaca ccaacaatca ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa
     6661 gagggatcga cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa
     6721 gaacactttt atcccacgct ctaaacacgt ataaacggcg gaagcaaaag caactccttc
     6781 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt
     6841 cttggagtta aacgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct
     6901 gtccttcggt attcgttcaa gccgctgatt tcacaacctc tccactaaac ggtgtactaa
     6961 agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat
     7021 ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga
     7081 ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac
     7141 gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg
     7201 gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca
     7261 gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac
     7321 tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata tcccgaataa
     7381 ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg
     7441 ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat
     7501 caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg
     7561 tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt
     7621 catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag
     7681 agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat
     7741 ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat
     7801 tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag
     7861 atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg
     7921 gcttgttcat tgtctacgac taaatgtttt gtttcatctg gtttgcccat catatggaac
     7981 acacgtgtat cttcattggc ataatcaaaa tagacataag cttccatacg acgttcatgt
     8041 gtatgacaag gcatcgtatt ccaagcactt cctggttcta aaatcgtata acccatttat
     8101 aaatgacagc tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga
     8161 taatgatgac aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag
     8221 ccatctgtcc tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta
     8281 gcatgattcc tagcgcccac aaa
//
LOCUS       DACEZN010000121         8066 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEZN010000121 DACEZN010000000
VERSION     DACEZN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.121"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..882
                     /locus_tag="IXH60_002148"
     CDS             <1..882
                     /locus_tag="IXH60_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1991049.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            930..3059
                     /locus_tag="IXH60_002149"
     CDS             930..3059
                     /locus_tag="IXH60_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1991050.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3034..3723
                     /locus_tag="IXH60_002150"
     CDS             3034..3723
                     /locus_tag="IXH60_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991051.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4131..4817)
                     /locus_tag="IXH60_002151"
     CDS             complement(4131..4817)
                     /locus_tag="IXH60_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1991052.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4953..5147)
                     /locus_tag="IXH60_002152"
     CDS             complement(4953..5147)
                     /locus_tag="IXH60_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991053.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5197..5637)
                     /locus_tag="IXH60_002153"
     CDS             complement(5197..5637)
                     /locus_tag="IXH60_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1991054.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5641..6486)
                     /locus_tag="IXH60_002154"
     CDS             complement(5641..6486)
                     /locus_tag="IXH60_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1991055.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6635..8053
                     /gene="pepV"
                     /locus_tag="IXH60_002155"
     CDS             6635..8053
                     /gene="pepV"
                     /locus_tag="IXH60_002155"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1991056.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1713 g         2357 t
ORIGIN      
        1 atttcagtac cggttgcact gttctcattt atgggctcga ttatggctgg aatctttgct
       61 aatatcgtca atttcaaaca atatcaaacg atttatgctg gtttcaatcc agcggctgaa
      121 agctatactt ataatgacta tttccaaatt ggtatgattg ggatgattgt aagcttagtt
      181 gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga aacgctatgc aatggcagca
      241 aacgtaccag ctgaaggtgg cgacgcacca atgatctcat ggttagccgt attattacca
      301 gttttagggg tcgttctttt agatcttcaa atcatcttag gttttattct agcaggtatc
      361 tgggcattgc tgtttactgg aaaattgcgc ggaggttata aagaaatctg tcgtcagttt
      421 gcgaaattat ttacagatgg cgcagtcgat gttgcaccaa tggttggttt cttgatgacc
      481 ttagcgatgt tcaataattc agctgcgtat gcttctcctt atttttcagc tatttttgga
      541 gattggatac cgcaatcacc attagtcttg gcaatcgtat ttgctatatt gacaccacta
      601 ggatttttcc gcggtccaat gaatctagtc ggttcaggct cagctatttt agctgtcgtg
      661 ttagctgtta atccaacgat gtcgccagcc tttttgttcc cgttatttgc aattacaacg
      721 attgctcctc agcatttaga tattacacaa tcttgggttg cttggggatt aggttataca
      781 aaagtaactt ctcgagaata tatgaagaaa tccatcccaa ctggttggat catcggtgcg
      841 atcttatgcc tgatcacgtt cttattgtac gggaacgcct agtaaacaaa acgtcgataa
      901 aaacgattag ataaatggag gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg
      961 ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa
     1021 aatcttcagt gaaaacaacc cacgatgatg tccgtggagt agctgcaggt gtcgttcagt
     1081 cttttcaaaa ttgtttgcgg gaaaataata tcagtccaga agatgttgtg tttgttgcac
     1141 attctaccac acaagcgact aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa
     1201 tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa acgacaaact agattgaacg
     1261 atatcgattt agggaataag aaaaaaatag agattgtcaa tgcattctta cctgtcaaaa
     1321 acttaaatgt agaccgtgtt tcagaaacga tttcttcttt agaaagagaa agagcggagg
     1381 tcttggtttc ttctatggca tttggagtag ataatgggga accagaacga gttgtttatg
     1441 aagcagcatc tgtgaaagcg atccctacaa cgatggcttc tgacatcaca aaattatatg
     1501 gattgacaag aagaactcgg acagcagcaa tcaacgcatc tattttacca aaaatgctag
     1561 atacggcaac ctcgacagaa gactccgtac gagaagcagg ggtcaatgtt tctttgatga
     1621 ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa
     1681 ccatgctttc cggtcctgct gcatcagtca tgggctcttt gatgtacttg agagcgtcta
     1741 atggtgttta ttttgaagta ggcggaacaa caaccaatat cggggtcatt aaaaatggac
     1801 gtccagcaat cgattattca attgtaggtg gccacccaac atatatctct tcattagatg
     1861 ttcgggttct aggtgtggca ggcggttcga tggtacgggc taatcagtca ggaatcatcg
     1921 atgtcggtcc acgttctgca catattgccg gtctagatta tgcagtattt acggagacag
     1981 aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa agaaggagac cctgctgatt
     2041 acgtcaaggt agtgatggaa gacggagaag aagtgactat tacaaataca tgtgcagcca
     2101 atgttcttgg attagtgcaa gaagaacatt tttcttatgg aaatgtccca tctgcacgaa
     2161 aagccataca ggcattagca gactattgtc atacgacggt tgaagacatc gcagagcaga
     2221 tcatggaaaa atcctatgct aagattgaac cggtgatttt agaattggca gacaagtatc
     2281 acttggagaa agatcagatt tctcttgtag gtgtaggggg cggcgctgcc tcattgatta
     2341 cttactttag taataaaatg ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt
     2401 cttctattgg tgtagcactt gcaatggtcc gagatgttgt agaacgtatc atcccttcgc
     2461 caagtaaaga agatatacga tcgttgaaaa atgaagcgat gaataaagcg attgaatcag
     2521 gcgctacacc agaatcgatc gaagtccacg tagaaatcga tccacatact tcaaaagtaa
     2581 cggctatcgc tactggttct acggaagtga aagcaaccga tctaaccaag gagatcacaa
     2641 cagaagaagc actagagctt gctgcagagg atatgcgatt aaataaaaat gaagtttgct
     2701 tattggaaaa tacacctttc ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg
     2761 gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt ccaacgtgga catgcttcat
     2821 gtatgaaaac aactgcagcc aattatatga cagctgtcga acaactttgg gaagatatgg
     2881 cagtttatca aactgaattg attgcccgac cagaatttta tctctgctta ggagcaagag
     2941 tctctgattt tacagcgacg gatctggaac aactacagct tttgatggat ctggaagtat
     3001 ctactatgga accggaggaa gaagtgatcg tcgttgccgg aaatatcaaa caaacataag
     3061 ttagaagagt tactaaacga attacaacag atccccgaag aaatttgggg attctaccag
     3121 tttcggcgag atttattttg gaaaaagatt cctttgtcca agcagaaaat tcttatacaa
     3181 caatcaatcg attgtggtat cgaaacggca tgtagcatca aaaaaaagta cccttttgcc
     3241 gatgtaggag aaatctgtga acaaatggct gttccaatcg tttcctgtga atcagagcaa
     3301 ataaacgaac gaatcacttt tgcaacttat gcagaagatg aagggatccg attgatgacg
     3361 gagccgttag aaaaattaaa atgttcagga ctagcttcga tctctaaaga aacagcgcaa
     3421 gctttgatta ttggacatga actatttcac cacatcgaag cttctgttaa gggaatttat
     3481 acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt atacacatca atcaaacatt
     3541 cgtgcattga gcgagatagc agcgatgagt ttttcaaaag agatgaatca aagcgatttt
     3601 tcgccttatg tattagaggc agtcttactt tggccatata atgaaattcg tagccaaggg
     3661 atcttagaag aaataaaaga aatagaaaaa agatgtacgg agtatgattt agattacaaa
     3721 taaagagact gtgacaaaag tcgtgtcaca gtcctttttt tccgaataaa cggtgttctc
     3781 ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac atgagaagct gttttttgaa
     3841 attgttcctt gtgactcgga gatgaacggc aggctcacga cctcttggaa aaaggtgttc
     3901 aaaagcttgc atctgcggta ataagaacag ccaagttaaa aacataagaa gctattttta
     3961 cttggctgtt cttattactt gatgcagggc agctttttca caacctctca ataaacggtg
     4021 tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg caaaatatga ggaactattt
     4081 tgccgtttat ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg
     4141 ggtctgtcct gaatatcttc aaaataatcg gggtaattat gaatcacgta ttcttgctcc
     4201 tcgataatca tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa gttatgatca
     4261 atcattgatt gaagcattgt ctgatgttca tctaatactc gaccccagtc taattctgaa
     4321 atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac tataactttc tagccaatcc
     4381 caaatttctc gttttccagc aatttcataa caagttcggt gaaaatcagt atccaaatca
     4441 aaaaaagctt ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct
     4501 agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc
     4561 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
     4621 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
     4681 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
     4741 ccgggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
     4801 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
     4861 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
     4921 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt
     4981 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
     5041 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
     5101 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
     5161 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
     5221 agcgtacgct tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg
     5281 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
     5341 ttcagttccc acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt
     5401 tgggtggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
     5461 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
     5521 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
     5581 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
     5641 tcatgcctcg ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc
     5701 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
     5761 atgaagataa acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
     5821 tgtgatcatc ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa
     5881 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
     5941 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
     6001 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
     6061 gggtagcaaa agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca
     6121 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
     6181 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
     6241 agtatccgtc caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
     6301 tttttcatca gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact
     6361 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
     6421 tcctttatgt gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
     6481 cagcatcgaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
     6541 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
     6601 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
     6661 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
     6721 gcgcgatgat tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
     6781 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
     6841 tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga
     6901 tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg
     6961 aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc
     7021 gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg
     7081 cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc
     7141 agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt
     7201 ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt
     7261 ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag
     7321 tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt
     7381 aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc
     7441 acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga
     7501 taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca
     7561 tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt
     7621 gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat
     7681 caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga
     7741 acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca
     7801 ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg aagaccacac
     7861 tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg
     7921 tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga
     7981 atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg
     8041 tttggcgaaa taaaaatact aaaaaa
//
LOCUS       DACEZN010000122         8042 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEZN010000122 DACEZN010000000
VERSION     DACEZN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.122"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(217..660)
                     /locus_tag="IXH60_002156"
     CDS             complement(217..660)
                     /locus_tag="IXH60_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1991057.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(878..2218)
                     /locus_tag="IXH60_002157"
     CDS             complement(878..2218)
                     /locus_tag="IXH60_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1991058.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2487..3383
                     /locus_tag="IXH60_002158"
     CDS             2487..3383
                     /locus_tag="IXH60_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1991059.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3533..4474)
                     /locus_tag="IXH60_002159"
     CDS             complement(3533..4474)
                     /locus_tag="IXH60_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1991060.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4600..5721
                     /locus_tag="IXH60_002160"
     CDS             4600..5721
                     /locus_tag="IXH60_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1991061.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5979..7481
                     /locus_tag="IXH60_002161"
     CDS             5979..7481
                     /locus_tag="IXH60_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1991062.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7548..>8042
                     /locus_tag="IXH60_002162"
     CDS             7548..>8042
                     /locus_tag="IXH60_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1991063.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFM"
BASE COUNT         2672 a         1508 c         1595 g         2267 t
ORIGIN      
        1 tcttaataaa cggtgttcaa aagcttgcat ctgcggtaat aagcctaacc aaacgaaaaa
       61 taggagaagc tatttttgtt tggctgttct tattacttga tgcagggcag ctttttcaca
      121 accccttatt cacggcgttc cagaagtagc ttcttcttat ttcttgaagc tgataacttc
      181 tggaacatcc tcttttttta tttggacgga taatggtcat tccatttctt tgatcatttt
      241 tttcattttg tcgatctctt cttcttgtgc ctgaatgatt tcttccgcta atttttttac
      301 ttcaggatct tttaaatcag ctcgtttact tgccaggata gcgatagaat gatgaggaat
      361 catggccttc atccaagctg tgtcgttgac agtagtttgg cttcgtacca taaacaagga
      421 accggcaaaa acaagaagac tagcggctaa gatcataaca tttatctttt tgttttcata
      481 cattttccac atgaacaaca gcatgatgac tgccatgact gcacccatca tcaacgccat
      541 gaatactcgt gtacgactga agaagatatg atcgagcgca tagacattca agtacatcat
      601 accaaacata actaccgttg aaacaccgat catcaacaaa aacttccaat actttttcat
      661 ttaaaacaaa ctcctttttt tcattccttt cttctatcat gtcatgagca tggagaacaa
      721 tcaattattt tgcacagagg gtttttgaac accgtttacc aagaggtcat gagactgttt
      781 attcggaaaa aggagttgca acaagacttt tgtcacaact cctttcttca atagctccat
      841 ctattccaca taaataaaat aaaattttct atcgatttca ttcattcgtc aatacgaacg
      901 catcattctt tttttgataa ctgaaataaa tgaacaggta aagcaggcca gtcaatacag
      961 ttaccccaat ggagttcgtg aacaggatac caaggaaaat caatcctaag acagctaatg
     1021 caaaaaagct gattccttta tcccataatg ttccttttct tagtgccagt acaaaggatg
     1081 ccgcactgat aatgatccag atcaataata ctgtatacga aagggaacct gccagatagc
     1141 caaataattt gtcaccgaca aaataagaaa gagctacacc aagataaaga acactggtac
     1201 aaaataaaac agcacgttgc ggtacttgat gcttgtttaa aacagctagt tttctagagg
     1261 aaccttttcg atccttcaaa cggaagaaca aaattcttga agatgcatac acacccgaat
     1321 tgatagatga gaacaaagca aggacaatca caaaattgac gatatccgct gcaaatggga
     1381 tattcatttt ttcaaatacc atcacaaaag gactgacatt cgtgcctgct aaagtattcc
     1441 aaggaaaaag aatcaaaagc aaaaacaaag gaatgatgta aaaggaaatg attcttccca
     1501 tgacaccttt gattgcttta ggaatcgcac gtttaggatc atctgcttcg cttaccgtga
     1561 tagcaatcag ttcagaaccg ccgtaagaat agataactac aagcaaagaa ctgatgattc
     1621 ccttcatacc atgaggagta aacccaccat ggtcagtcaa ttgctgaagg gtagggaaaa
     1681 cgcctgtgcc caacatttgc tgaccgacaa gataagtagc aaaaatgatc agtaagataa
     1741 tgacactgat tttagcaaaa gccagccaat attctgtttc tgcgaataac cgtaccgaat
     1801 aaagattgat gatcgtagtt aaaacagcca acataaaaac aaatacccag gctggaatgt
     1861 gcgggaacca caactgcaaa aaggaagcag ctgcaacagc ttcagcgatg atattgataa
     1921 tccacataga ccagtagacc caatcagtaa aatcagcaga atgagcacct agataagggc
     1981 gtatcaaacc agataatccg tgatgcgtgt cgccaccgct taaaacgatt tttcccagac
     2041 ctttcatcac gatatataaa atcagcccac caatcaaaaa agcaatcaat acagaagggc
     2101 cggcgatccc aatcgcttcg ccacttcctt taaatagacc tgcaccgatt gctccaccta
     2161 aagctaacat ggtgatatgt ctggagctaa gttgtttttt caatttttta tcttccataa
     2221 atgacctctt tcttttaaat tcacaatcaa aatttatatt aagaataaat agcaatattt
     2281 aacaatgact aatgataaca aattttcaga caattacaac tattcagaaa acttttaggc
     2341 gaattttaat agaagaatga tttatttcat catattttca tacaaatgtt aaattttcat
     2401 acaaatatag gaaacgtttt actggctttt tactatttaa acaggctatc ataggaaaaa
     2461 gtgacagaga aagtgaggcg gaaaagatga acaaaaatga cgtactgcta gttgcaaaaa
     2521 ataaatcact ttattttttg ccttccatcc gctctttaga tgtttggtcc ggaaaagatt
     2581 tactacagtc ggaattcatt aaatcagcta gacaacagtt ggcaaattat cagctgatcg
     2641 tttttttaga ttatggattt gatatggaga tggctcgacg cgtgcgccca tttaccaatg
     2701 caaaaattgt gttatttttt tggaatcact tcaaagcaga acacgaggcg atgctgaagc
     2761 aggcacaaga tgaaccggca atcgatgaga tttatcattt tgattttttg gaagcaaaag
     2821 aacttgggct gaaacataat agttcatttt attcgaaaca tatgaatttt tcttctagcg
     2881 aaccaaagac ggatctattt tttggtgcaa cgaataatgg gagaaaagaa cgagcagaaa
     2941 gctataagca ggaatttata aaaagagatg ttacgaccaa ctatttcatt cttccacatc
     3001 gagggaatga acaacctgga tatcttactt atgaagagta cctgaaaaaa acaggggaat
     3061 cgcgcgggat tctggaactg ttgcgggagg gtcagcaagg agtgactttg cggacatttg
     3121 aatccatgta ttttcaaaag aaactagtga cggataatca agcgattgct cattatcatt
     3181 tttatcgtcc tgaaaatatt tttttgcttc gagaacgtga tttagatgaa ttaccggaat
     3241 ttctgcatac accatttaag ccaatcctat cagagctatt ggaattttat gatgcagaaa
     3301 attgggcaaa acgatttatc actaacgatt ccactattta tgagcaatac gaatatgacc
     3361 tctcctctgt tgaaaacaaa taaaaaacct acattttgaa gagattatgt actgaaacaa
     3421 acaagcacaa ttcttatggc atcatgagag ttgtgttttc tttttgatct tttattgggc
     3481 ttaaaaaaag agaaccagct gctgattcag ctgattctca tcagagagcc ttttattctg
     3541 cgccaaggat tccttctttt gcatggacta attgggtcaa aaaggcacaa tatggtgtag
     3601 caatatcata tttttgtcct tttcgccaaa tggcaccatt gatgtagtcg atttcagtca
     3661 aacgatggtt tttgatcagg tcttggtaca tagatgggta atgcaacccg atgttgtcag
     3721 gatcataagt agaagcaata tgttggtaca cttcttcttg atctagtatg attccttctt
     3781 ttgctgcgat agaagcaaat tcagatacga ttgttcgaac catgctctct gccgcttttt
     3841 gcgcgcctag ttcagcaata ttacaatcca aaatcgtgca taaaccattc aatgttccat
     3901 ttacacaagc ttttcgccaa atggaataac gaacgttatg gctatagatc ggatttaaat
     3961 ttgcttcttg aaagacttct acaacttttt ttgtaaatgc ttcgccttct ggttccaagt
     4021 tctctagttc gatttctcca tcacctaaca atgtgacttt tccgggacca gctagaccag
     4081 ctgtccacat cgtgatccca aataagatat ttttctttgg cacatatttt tccagtacat
     4141 cttcatgacc taatccattt agtaaacaca aaacataagt attgtcagta ataatggatt
     4201 ggatcgaaca aaacatatca tctaattgat tggcttttgt taatgctacg atcaagtcca
     4261 cgtgttcgtt gctttcgatg atttcttctg gtgaatagat tggcagtttt gcgaccactt
     4321 ctttaccgtt aaagtcagca atcaacccat ttgtacgaat ggcttcaatg tgagcaggcc
     4381 aacgatcgat caagaggacc tcatttccgc tttgatgtag catcaatccg attcggctgc
     4441 ccattgctcc ggccccagct attgcgattt tcatgttttt tctctccttt attcaatgag
     4501 ttccctttta gtatatagaa aaatggaaga aaatagcatt gttttgtttg aaaaaaatga
     4561 aaaaaagtgt cttacttaag aagaaagtga gggatacaga tggatatttt ggcggcaatt
     4621 gattcattta aaggaagtgc ttcttcattt gaactaaatc aggcagttct aaatggctgg
     4681 gaagatcgta aaggaagaaa aatcaatgtg ccgattgcag atggcggaga aaatactgca
     4741 gaagcaatct attacgcgct caggggaaca tggcgtacca ttgatgggat ggatctctta
     4801 ttccgtcagc gaaaaatcag ctatttattg acaacttggc aaggaaaaaa caccgcagtc
     4861 atcgaatctg cacaagcatt ggggttagat ctacttgata aaccaacgga tcaaactatc
     4921 cgacttgctt ccagttatgc cttaggagaa cttattaaag atgcactgct tcaagaagta
     4981 gaacaaatta ttgtcacttt aggaggaagc gggtgtagcg atggtggtct aggattactc
     5041 caatccttgg gtgcttcttt agaagggtac acagaaggaa atcctttatt atctactaaa
     5101 aaacttcatt ttgatcgaag aaacgttctt gtttctaaag taaaattgat tatcgctgca
     5161 gatgttacca atccatatac aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa
     5221 ggaggaatag atacgacgct agctttctta gataaacaag caggaaagat tgctcagcaa
     5281 ataaaagatt tctgggaggt ggatttagat gatattccag gaagtggagc agcaggcggt
     5341 attggtggag cccttttttt gttgggagcg aagatggttt ctggtttcga tttagtcagc
     5401 caaatcactg gattggaaaa aaagatcgaa caagcagatg tcatcattac aggagaaggg
     5461 cggatcgatc agcaaacgat ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa
     5521 aaatatcgaa aaccaataat cgctatttgc ggaagtagag accaagagct agaaggactt
     5581 tctgattttc tcccaattgt cttaagtatt catttgggtc caataggttt agaagaagca
     5641 atggaacaca ggcaaacatt agaaaaaaca aaaatcttag gacagacact tagcagattt
     5701 ttcaccatcc tcccgaagta gttgacaaaa tcattttctt tatgctatcg taatttcaaa
     5761 tataggacaa agtgagggat ccgtgcattg aaaaattaag tcgattgatg aaagctgcat
     5821 tcagactgta ccttgtggag acaaggaagc agtgtgcaga ttttcgggat tggcttggtt
     5881 ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg catcctcttt agccagttct
     5941 gaaaatagga ctggtctttt tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa
     6001 atctgacatt cggctacgac agccaaggca cattattatt tgaacaagca aatctaaatt
     6061 ttgacacaca atggaaacta ggacttatcg gacgaaacgg tcgaggaaag acaactttac
     6121 tgaatattct acaaaacaaa ctaccttatc aagggcaagt aatccatcag caagaatttg
     6181 cctatttccc gcaacagaca aaagataaag aacgtttaac ctattacgtg ttaaatgata
     6241 ttacggattt tgagatatgg gaaatcgaaa gagagctcca attgatgcaa acagatcctg
     6301 aaatcttatg gagagaattc agcacactat cggggggaga gaagacaaaa gtcctactgg
     6361 cacttttatt tgtggatgac actcatttcc cgttaatcga tgaaccaacg aatcatttgg
     6421 atatctctgg tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg
     6481 tggtcagcca tgaccgggga tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa
     6541 aaagtcaact ggaactttat caagggaatt tctctatcta tgaagaacag aaaaaacttc
     6601 gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga
     6661 aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga aggagataaa acaaagaaac
     6721 aagtcggatt catcgatact gaatctagac gagtgaataa aggagcagtg ggtgctgatg
     6781 ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg
     6841 agaaagaaaa actattaaaa gatatcgaat atatcgattc tttgacgatg aatagccaag
     6901 cgtctcacca taagcgactt ttaagcgtag aagatcttca attagggtat gaaaatctgt
     6961 tattcgagcc aattcatttt acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta
     7021 acggtgcagg aaagtcatcc attatccatt atcttctggg ggcattcaac ggcaaggtta
     7081 taggagaaaa ataccagcca aaacatctga gcattagtta tgcaagccaa aattatgaag
     7141 acaatcgagg aacgttggcg gaatttgcag agaaaaacca agtagactac caagcatttt
     7201 tgaacaacct ccgaaagctt gggatggaaa gagatgtttt tcataacaag atcgagcaga
     7261 tgagtatggg ccaacggaaa aaagtggaat tggctaaatc tttatcacag ccagctgaac
     7321 tatatacatg ggatgaacca ttgaattatt tggatgtctt caatcaagaa caattagaac
     7381 aactgatctt gaacgtgaaa cctgccatgt tactagtgga acatgatcaa accttcctgg
     7441 ataaagtatc tactgagatt atttctcttg agagaatcta acccttgtaa tctcaagaac
     7501 aacaaggtat ggttaagggg aactttgcca acgaaggaga gaatcacatg ctttatgtaa
     7561 cgagacacgg agaaacaact tggaacgcac aaggattagt ttgcggacgg gcagatgtgc
     7621 cgctgactga aaaaggacag atgcaggcac agaaactagc agaaaaggta gtagatttgc
     7681 ctgttccaat cacgaaaatc atccattccc ctcttcaacg agcaagagat acagcacaag
     7741 cagttgctga tcgtctcagc ttgccgctga ccgttgatga acgattggta gaaatggatt
     7801 ttggggatta tgatggactg ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt
     7861 tcgctgtacg attcccaaat ggggagtcag tcttagatgt gtatgcacgg atcgttcctt
     7921 tattgaaaga atgcatcgaa gacgaagaaa atgtctatct gttagtttgc cataatgcct
     7981 tgataagagt gatcaatgct tattttcatc cgatgcctaa tgaggggttc tttacattca
     8041 tg
//
LOCUS       DACEZN010000123         8019 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEZN010000123 DACEZN010000000
VERSION     DACEZN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.123"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..642
                     /locus_tag="IXH60_002163"
     CDS             <1..642
                     /locus_tag="IXH60_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1991064.1"
                     /translation="ELIEMTRSFCEEHQLFSFDVMTPPISEIERLTGIKPIRQPGALH
                     LLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSKTPLSLFLANKNLKVA
                     NLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSYGLNPDTAYSDIEKIR
                     AELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSYYGTETTEETD"
     gene            complement(737..1030)
                     /locus_tag="IXH60_002164"
     CDS             complement(737..1030)
                     /locus_tag="IXH60_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1991065.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(1103..2830)
                     /locus_tag="IXH60_002165"
     CDS             complement(1103..2830)
                     /locus_tag="IXH60_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1991066.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(3044..3970)
                     /gene="trxB"
                     /locus_tag="IXH60_002166"
     CDS             complement(3044..3970)
                     /gene="trxB"
                     /locus_tag="IXH60_002166"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1991067.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(4115..5548)
                     /gene="ascB"
                     /locus_tag="IXH60_002167"
     CDS             complement(4115..5548)
                     /gene="ascB"
                     /locus_tag="IXH60_002167"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1991068.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(5548..6951)
                     /locus_tag="IXH60_002168"
     CDS             complement(5548..6951)
                     /locus_tag="IXH60_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1991069.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            7181..>8019
                     /locus_tag="IXH60_002169"
     CDS             7181..>8019
                     /locus_tag="IXH60_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1991070.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFD"
BASE COUNT         2198 a         1791 c         1411 g         2619 t
ORIGIN      
        1 gaattgatcg agatgacgcg ttctttttgt gaagaacatc aattatttag ttttgatgtg
       61 atgactccgc cgatatcaga aatcgagcgc ttaactggta tcaagcccat tcggcaacct
      121 ggcgcccttc atttattgaa tgagaactat ttcaaacgga ttaaagcaat ggaatttgct
      181 gtaaaatacg atgacgggaa agatcctcgt gggtttctag aagcagatgt cgttttgcta
      241 ggcgtatccc gaacttccaa aacgcctttg agcttgtttt tagctaacaa aaatttaaaa
      301 gttgccaatc ttcctttgat cccacaagca catattccca aacagctttg ggagattgat
      361 cctaaaaaaa tcgtgggatt gacgaataac ccagatattt taaacaatat ccgtaaagaa
      421 cggatgcgct cttacggatt aaatcctgat actgcttatt ctgatatcga aaaaatacga
      481 gctgaactcg attttgcaaa tgatctttat gaaaaactgg gctgcgtagt aatcaatgtc
      541 gcttctttat ctattgaaga aacagcttcg cttattttga atgctttaga cctagaagat
      601 catagttatt atggaactga aaccaccgaa gaaacagact aacagttttt cttgtctttt
      661 tcttaaaaaa aactggactg tgtcagcgaa gaagattcgt tgacacagtc cagtttgtct
      721 ctatttgcac ctatcgttat tgatgttcca catgtttgag tgtctggttc aaaatatcca
      781 gtacgtgaga atcatcaaga ctatacaaaa tcgcttttcc ctcacgatgg gacttgacga
      841 catggttttc tcttaataaa cgcagttgat gcgatacagc agattgttcc atttccactg
      901 cttgggtgat tgctgtcaca ttttgttccc cgtctttcag atacagcaga attttgagac
      961 gagtggggtc actcaacatt ttgaatagtt ggctgacttt ctctactttt gttttttgcg
     1021 tgtctggcaa tccattcacc tccaacttga aaaaagactg acttctaaag tttaccactt
     1081 tttccatcag tctcaagttt ttttattctt ctgtcatctt ttggataatc gcttcataag
     1141 cggctacttt ttcagctgcg tcttctgaag agtttccttt tacaccgatg taaaatttga
     1201 ttttcggctc agttcctgaa ggacggatag cgatccagcc attttcttcc agcgtatact
     1261 tcaatacatc agatggcggt gtcgttagtt tttctacgtt tccttcacgg tctgttctcg
     1321 tcaactcttt gaagtcttct gtttggatga cttttgctcc ggcaaattct gttggtgctt
     1381 cttcacggaa tttttccatc aaagcagaga ttttcgcaga accttcttgt ccgctcattg
     1441 tcacagaaat cgttttttct gcgtagtagc cataggtttc aaaaatcgtt tgcaagccgt
     1501 catacaacgt tttgccttgt tttttatagt aagctgctac ttctgctaat aagagcaatg
     1561 cttggatcgc gtctttgtcg cgaacgaaag gtttaactaa ataaccgtaa ctttcttcga
     1621 atccgaacat aaaagtatgg gaatgatctt cttcaaattg ttgaattttt tcagcaatga
     1681 atttgaatcc tgtcaataca ttgaacatgg tagccccata gtgttccgca attgctgtag
     1741 gtaattcgct agaaacgatc gatttcaata ccgctgcgtt ttcaggtaaa gtgcctcgcg
     1801 attggtgtgc ttccaaaata tattgagcaa acagtgctcc gatttggttt ccggtcaata
     1861 cttcatagga gccatttggc aaacgaacgg ctgcacctaa acggtctgca tcaggatctg
     1921 ttgcaatcag caaatcagcg ccttctttct cgccaagtcg tatcgcatac tcaaaagcag
     1981 agtgttcttc tggatttggt gatttgactg tagtgaaatc tgggtctgcg acagcttgtt
     2041 ctggaactag tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg cccaacattt
     2101 ttcctgttcc gtgaagtggt gtgtacacaa gtttcaaatc tttccccatt gtttccacca
     2161 attcatgatc aatcgttact gctttgattt cttctagata agcggcatcc acttcttctc
     2221 cgatgatcgt gatcaatccg ctatgttcag cttcttcgtc agataatact tcaactttta
     2281 acggattgct gacttcacgg acatatttcg tcaatgcatc tgcgtctgca ggaggcattt
     2341 gtcccccatc ttcgccgtag actttatatc cgttgtaagc tgctggattg tgggatgcag
     2401 ttaccatgat tcccgcaaac gtcttcaaat gacggactgc aaaagaaagc tctggtgttg
     2461 gacgtagact ttcgaataca taagatggga tattgtgatg tgctaatgtt ttggctgctt
     2521 ccatggcaaa ttctggtgat tgatggcgtg agtcataagc gatagcgaca cctcggcgtt
     2581 ttgtttcttc gccttgtgta tccatgaaac gtgcaagtcc ttctgttgct tgtcttaccg
     2641 tatagatgtt cattcgattg atcccaggcc ctaagattcc ccgcatgcct gcagtaccga
     2701 attccaacgg tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc agatcattta
     2761 attgtttttt taagttttct tctaaatttt cttcatgtac ccattgttca tacacttgtt
     2821 cccaagacat gaaaataaca cctctttctg aaatatggtt cttaataagt atagcattca
     2881 cttattagat ataaaagtat gaggaccaaa aaacgcttaa atcaagaaag gattctttta
     2941 tcaagcacgg ttgcaagtat tcgcataaat cggattcagt agatttcacg cgaaaaaaaa
     3001 gagtaaaaga acaattgaaa ccgttcttcc actcttttta aacttatgca gttgtttctt
     3061 ctaaagattc caagtattta tacacttgtt gtccagcgat cccaccatca ccaacagcag
     3121 tagtgatttg tcgcaaatct ttttcacgaa catctccgat ggcaaaaacg cctggtatat
     3181 tcgtcttcat ttcgttgtct gttggaatcc agccagcttc gttcgtcaac cctgcttctc
     3241 taaatggtgc agttaatgga tccagtccaa catagatgaa tactccgtct gctgccaaat
     3301 caaactcatt gccggttttg acattacgtg ctttcacacc tgtcactacc atttcattcc
     3361 caacgatttc gtcaacgact gtatcccaca taaaggaaat tttttcattc gcaaatgcac
     3421 ggtcttggat gatcttttgt gcacgtaatt catctctacg gtggacgatg actacttcag
     3481 aagcaaattg tgtcaaatag atggcttctt ctactgctga gtctcctcct ccgatcacta
     3541 ctagacgttt gttacggaaa aacgcaccat cacaaactgc acagtaagaa acgccacgtc
     3601 cagcaaattc ttcctcgcca ggtacgccta atttacggtg gatacagcct gttgcgataa
     3661 tgactgtttt tccttcgtaa gttttatctt cacaaataac ttctttgtgg ctgccgtgat
     3721 ccttgatatc ttgcacgatt ccataagcat tttctgtccc aaatcttgaa acaccttcat
     3781 acatcttata agctagatca ggacccatga ttgaatcgaa accaggatag ttttcgattt
     3841 ctgccgtatt gttcatttgg ccgccagggg cgccttgttc gatcatcaat acggataagt
     3901 ttgaacgtga agcatataaa gcagcagtca tcccagcagg acctgcgcca ataacgatta
     3961 catcatacat atgtagattc tcctttactt aatcaagtaa gcatacttta cagccaatgc
     4021 gaaaaaaagt ctattgttct gcttgcgttt ctcgaatagc tttattatac tcgaaaaatc
     4081 cgctgaaaaa caatagactt ttataaatca ttgatcaatc attcgaaaga ttttctccat
     4141 ttgaatcaat tactttttta taccagttga atgatttctt cttcgaacgg gctaatgtgc
     4201 catttccctc gttgtcacga tcgacataaa tgaatccgta ccttttcttc atctcacctg
     4261 taccagctga tactaaatca atacatcccc aagtcgtata gcctaataga tcgactccat
     4321 ctaattctac tgcatctttc attgcttgga tatgtgctgc cagatagtcg attcgataat
     4381 catcttccac ataaccattc tcatcgggcg tatcgactgc acctaagccg ttttctacaa
     4441 taaatagtgg tttttgataa cgatcataaa tatcattcat cgttattctc aagcctaatg
     4501 gatcaatttg ccaaccccat tcacttgctt ccagatatgg atttttgaca gaagcaaaga
     4561 tatttcctgc agtctgttcc aagagttcgt tgtcagtagt cgaaactcga gaagaataat
     4621 aagaaaagga gataaaatca acagtatgtt ctttcagcaa tgcggcatcc ccaggttcca
     4681 tttcgatctg aatgccattt cgttccattt ctttcaatgc ataagcggga tattctcctc
     4741 gtgactggac atcaatgaag aaataatttt ctcgatctga ttttctagaa gcccaaacat
     4801 cttcaggatt gcaagtgtat ggataatatg ctcctgctgc cagcatacag cctactttgt
     4861 tctccggatc tacttcatga gcaatcttcg ttgcgatggc acttgcaacc aactcatgat
     4921 gtgccgcttg atatttgatt tgttcttcat tttctccttc ttcaaaatac agccctgcgc
     4981 ccatgaatgg tgcatgaagg atcatattga tctcattgaa tgtcaaccaa tatttgacaa
     5041 gtcctttata gcgattgaag atgactcggc acagattttc ataaaaacca acgagttctc
     5101 tggaacgcca cgcaccatat tctttgatca aatgcatcgg acaatcaaaa tgtgtaatcg
     5161 tcacaagcgg ctcgatcccg tacttttgac attctttaaa gacatcctca taaaatttca
     5221 acccagattc atttggctct ttttcatcgc ccattggaaa aatacgactc caagcaatgg
     5281 acatccgata agtcttgaac cccatttctg caaataaagc aatatcttcc ttataatgat
     5341 gatacatatc gatcgcaatt tttgctggat aaaaatgatt ttcatcaaat tcaaacattt
     5401 tcttttttcc agtaatgaca ggaaaacggt cttctccgat tggcacaacg tctacgttcg
     5461 ctaacccacg tccgccttca tcataaccgc cctcgcattg attggctgct gtagctccgc
     5521 cccataaaaa atcttttctg aatcccatca tacttcctcc attacatgtg tattgcttga
     5581 taattctctt gccagttcat cttggacttc gattccttct gcttttaacc ggctgattgt
     5641 ttccttgaat tgcggtaaat actgctgatg tgcaataaac aactcatcca ttatccgttt
     5701 tgctgtcttt cctgatcgta taagcggatt gatcgtaaac gcttgcaaag ctgttccata
     5761 atcaccagtc acggctgctt cgatcgtcag caattccata ttcttcatta cttgcaacca
     5821 gcctctttca gcagcaggaa gggaaccaaa cgctacattt cgtgcgcctt gtgcgccaac
     5881 ataggcagtg acttctacta cacaatctgc cggcagatca ggaacagctc catcattctt
     5941 tgtagatacc acgatctgtg tttctttatt tccatagatc gaagcaatcg tttcacaggc
     6001 agcatctgaa taataagcac cacctcgttt ttgcaattgt tcaggtttat gatccaattc
     6061 tggatctttg tataattcaa acagctctgc ttctgtttct ttcacttgct gcgctcttgt
     6121 accaatcgtt tgatattctt ctaaagcatg ttccagcatt tcttcttcac ggtagtaata
     6181 gcggtggtag ccacaaggaa tcatgttcat ttgttttaat tgttctttga aaaacgggat
     6241 atcaaagata tttttcggca atccattgtc ttcatcgtat aaacgatcaa tcaattctgc
     6301 cgtgcggtca tttcctttat tatctgctac tttgtgccaa tggaagtgat ttagaccggc
     6361 aaacttgtag atcagctctt cttttttctt gccaagcatt gttggttctg tcatcatcgc
     6421 cattactggc acattgcata atccgatcac tttatcccat ttgccatatc tgataactgc
     6481 ttctgtcacc atcccgcttg ggttcgcgaa attgatcagc caagcatttg gacataaacg
     6541 cttcatgtct tcaacgattg ccaaaaccac tggaacggta cgaaaagctt tgaatatccc
     6601 gccagcacca tttgtttctt gcccaatcat cccataataa ctagggatcc gctcatcttt
     6661 gattcgtgca tctaattgac cgacacgaaa ctgagtcgta acaaaatccg catctttcaa
     6721 tgcttcttcc cgatccagag ttgcatagat tttgacttca tacggagaag catcccacat
     6781 acgctgagac atctgactga cgatctcaag cttttctttt ccttcttcta catctacgag
     6841 ccagatttct cgtatcggta attcctcata tcttttgata aatccttcca tcaactcggg
     6901 ggtgtaactg ctgcctccac caatcgtagc gattttgatt ccttttgcca tgttctcact
     6961 cctttacttt ttacactttt attatctttt ttgtaaacaa aacaaacaat agaaattcgt
     7021 ttctttccag taacatttac aaaagttagt gttttcttct ggatttttgt aaatatcttg
     7081 tcaaaaaatg tctttattaa gctatataac ataaatccgt ttacaaatca ttttcaaaaa
     7141 agttataatt tacgtaaaaa aacaagaaga aggtatctac atgttattac gtgaaaaaat
     7201 caaagaaatc tccttttctc cttcagaaaa tgaagtgatt cagtttctct taagcgattc
     7261 tccagcactt ggcgaaatga ccatccaaga gattgctgag cgatgttatg tccacccgtc
     7321 cacactcatc cgtgtagcaa aaaaaatggg ttttgatggc tgggtcagtt ttcgtaatgc
     7381 ttttttagct gaaacagcct atcttgaagg gaattttcaa gacgtagatg cgaatcttcc
     7441 tttttccacc acagacggta ttatgacgat cgcaaataaa atggcttctt tagaacagat
     7501 gacgattcaa gatacacttt ctctcattca tcatgaccaa ctgcaaaaag ccaaacaact
     7561 aatgcttcaa gcaaaaaaaa tcgtgatttt cgccagtaac tcgaatactt tgatttctca
     7621 agatttcatg ctgaaaatga agcgcatcaa aatggatgtc acgattgtca ccacttttgg
     7681 tgaatcgttc tatgaagcgt acaattgcac gagtgatatg tgcgggattc tgatttctta
     7741 tacgggtgaa aacaatgtga tccttcgtac cgcagaaata ttgaaacaaa atcatgtgcc
     7801 aattttagct ttgaccagta tcggtgattc aacgctatct tcaatgagta cagccgcttt
     7861 gcgacttacg acacgcgaac gactgtattc gaaaattggt aattttacga tcaatacatc
     7921 gatttgttat cttctagacg tgttatatag ttgtgttttt gcagaaaatt atcaacagaa
     7981 tttagtccat ctaatttctg taggacaaaa atttgataa
//
LOCUS       DACEZN010000124         7941 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEZN010000124 DACEZN010000000
VERSION     DACEZN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.124"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(733..2079)
                     /gene="gor"
                     /locus_tag="IXH60_002170"
     CDS             complement(733..2079)
                     /gene="gor"
                     /locus_tag="IXH60_002170"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1991071.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2213..3454)
                     /locus_tag="IXH60_002171"
     CDS             complement(2213..3454)
                     /locus_tag="IXH60_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1991072.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3472..4395)
                     /locus_tag="IXH60_002172"
     CDS             complement(3472..4395)
                     /locus_tag="IXH60_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1991073.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4616..7108)
                     /locus_tag="IXH60_002173"
     CDS             complement(4616..7108)
                     /locus_tag="IXH60_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1991074.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7115..7582)
                     /locus_tag="IXH60_002174"
     CDS             complement(7115..7582)
                     /locus_tag="IXH60_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1991075.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7718..7791)
                     /locus_tag="IXH60_002175"
     tRNA            complement(7718..7791)
                     /locus_tag="IXH60_002175"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7755..7757),aa:Arg,seq:acg)
     gene            complement(7849..7922)
                     /locus_tag="IXH60_002176"
     tRNA            complement(7849..7922)
                     /locus_tag="IXH60_002176"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7886..7888),aa:Pro,seq:tgg)
BASE COUNT         2217 a         1718 c         1284 g         2722 t
ORIGIN      
        1 gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatc agaggttgtg
       61 aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggctt tccacatttt
      121 tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat cagaggttgt
      181 aaaatcagtg ttttgtcctg agtattagag aaacaaatgg aaaaatagtt cctcatattt
      241 tgccgttttt tgagctaata ccgaaggaca gctgattgaa cactatttgt tcggaaaaag
      301 tgactaacta tgacacaatt tttttcacag tccctttttt cacttgattt gattcgtacc
      361 agcgaacttg acgagaactt tcttggtttg gtaactatat ggtattcttt taacatcctc
      421 gtttgcttct tctaatttat ttatacaaag tcacactttt ttaaacatct gttcttcgat
      481 acatttctat ttcttttaaa catcgatacc aatattttga acttaagagt gcataccaaa
      541 tggattcatt tactcttcgt cagttacgaa gataatcaaa atctccaact tccctactta
      601 tttcgttttg agaattatcg aagcaatctt acataaataa ctaaaaaaca aaaaaccaga
      661 aaatcagtat actgattttc tggttttaca tggtttgttc aataaaaagc tgttggaact
      721 gttaatatta aattatcgca tcgttacgaa ttcttccgct cctgttggat ggatggctac
      781 tgtattgtca aagtcttctt tagttgcacc catcttgata gccacggcaa aaccttgcag
      841 catctcatcg acaccaacac caatagcatg taagccgacg attctttctt cttttccgac
      901 acagatcaat ttcatttcgc atttttggcg ataatcattt aatgcaaagt acattggggt
      961 aaagcgtgaa cgataaactt tcaattcatt ttcgccatat ttttccaatg cttcttcttc
     1021 tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg actgtcggaa caagtttata
     1081 gtcaagataa gaatctttct ttccattaaa taaacgttct gataaacgtc taccagctgc
     1141 gattgcaaca ggagtcagat ctaatttacc gatcacatca ccaacagcaa aaatatgttc
     1201 atccgttgta ttttggtatt tatctacttt gataaaccct tttttgtcca attccacatt
     1261 tgtattttcc agacctaatt tttcggtatt cggtacacgt ccaccggcaa aaagtacaca
     1321 atcaccagtt aaggtttcac cattttcaaa tagcaccgta tattcgtcat tgtttttagt
     1381 aatttcttta gcaatatgat gtgcgtaagt ttttattcca gcttcttggt acatttggat
     1441 caaattgtcc gaaagcattt tatcaaacgt tctaagtggt cgttcttttc gatatgccca
     1501 catgacttca ctacctaaac cacgaaatac acctgaaagt tcagccgcaa tgtatccagc
     1561 acctaaaacg attgctctct taggtaactc tgttaattca aaaaatccat ttgaatcaat
     1621 tgcgtattct ccaccaggaa tggtcatttt acttgcacgg cctccagtag cgatcaagat
     1681 tttaggagca cgataggttt caccgtttac ttcgatcgtt tgactatcga taaaagtagc
     1741 gtaacctttt atagcctcga catgattgct atctaatcct ctttgataag caccgtgtag
     1801 aaaatcaata tatttttctc gtctttctac tagaccggcg aaatcaacat tttttaaatc
     1861 tgcttggata ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg tacttgcttg
     1921 ccacataact tttttaggta cacaaccgac gttgacacat gttccgccca attcattcgc
     1981 ttcaatcagc aaaacttttg cgccatgcat ccctgctctg tttgctgaag caataccgcc
     2041 agagccgcca ccaattacga tgtaatcata tatttgcatt ttgttcctcc ttattgaaga
     2101 gatcaatcaa aacctttatt taacgatcct ttttctacag aaaacaagtg taacactttt
     2161 cttgaatttt gtatgcgaag atgcttaaaa cagggatata tcagcttttt ccttatttac
     2221 gttttcggat catatctcca ggtttcaccg gctgtgcgac tggcatagtt aaaatcatca
     2281 ttggatttgg tgctcggtca atggattcgc cttcttcatt atgcataact tcaacggttt
     2341 gactgaaatg actgaatcct ggaccataaa attcgatttc atctcctaca gaaaacaagt
     2401 ttctctgacg tatcgtagca atctgtgttg cctcatcata agataatact tcaccgacaa
     2461 atttatatac tgggatttta cgacgttcac cgaataattg ttcgttttca gttggtgtat
     2521 tgtagtaaaa acctgtagct aattctcgtt gtgctacttt ccataactcg tcgatccatt
     2581 cttgcttgca gacatagttt tcaggatctt ccatataact gtctacagcc gctttataga
     2641 catttgacac cgtagataca taatggatcg atttcattct tccttcgatc ttgaagctgt
     2701 ctacgccgtt ttcaatcaat tctggaatat gttcgatcat tgacatatcc actgcactca
     2761 tcgagaattc ttcttccact tcgcctgtat cagttaaact gtttcgttct tgaccaaaag
     2821 gcatatcgaa caatccatac ttccagcgac aagattgaga acatccgcca cggtttgcat
     2881 cccgcatcga catatggttg gacaatgtgc atcgaccaga ataagagata cacatggcac
     2941 catggataaa tgcttcgatt tcggtatccg tatttttacg aatctctgcc acttcctcca
     3001 ttgaaacttc acgagctaaa acgacacgtt ccaacccttc ttctttccag aattccaatg
     3061 tctcaaaatt agttgctgaa gcttgagtgg ataaatggat cggcagacct ggggcttctg
     3121 tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac tgcagaaata ccgacatcac
     3181 gcaaactgcg gaaaaattcc cctgctccat cttcatttcc ttcatgggtg accatattag
     3241 cagcaacgta taccttcgca tcatgttctt ttgcaaaagc aactccttca gccatttctt
     3301 cataagtaaa attacctgca cggcttctta aaccatatgc attcccgcca atatatactg
     3361 catctgctcc ataatggatc gcggttttta acttttccag tgttcctgct ggcgctagga
     3421 tttctggtcg tttcaatttt ctttttactg tcatttcttt catcatactc cttatttgat
     3481 agcagaagga tcaatataaa agaatcctgt atctaattca cggttttcag gatgcaattt
     3541 actgatttcg ctagtcattt tttccgcttt ttcttgatcc cagctacctt ctaaaatttg
     3601 tttttttgct tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg gagtaaataa
     3661 tccgtccaat ttccatgtac agtaatgatg ctgataaagt tcattcaatt cattcgagag
     3721 attcagatca ttgctggcaa aaatatgtgt gccatgacta tcttcataaa tagaataatg
     3781 tgtctcttct tttttaggtt ccgagataaa aagacctctt tcacgatctt tttgttcgtc
     3841 ttgtttcgta tagttatagt aattctgtag cagtggacgt ttggattgat ggatgcatgt
     3901 agcaccatac acaagggttt ctacaggaat atcgagtttt tcttccatcg ccttcatctc
     3961 ttcaaaagga acttcacgag caagtactgc tccaactgcg ccttttttcg cccagaaatt
     4021 gatttgacgt gcgctcgtta ctaatgtttc agcatcataa atatacggta tacgcaattc
     4081 aggatttttt ttcatcgtat aaataatacc tgtatcacca actgtgatca catctacgtt
     4141 tatttctttt aaaaaagcca aatattcagg tacatttttc attttttcag gatgaatgat
     4201 cccatttaca gcaatattcg cttttttacc atacttgtgt gctaattcta ccaattgtcg
     4261 ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca aattgctctt caccgaaata
     4321 tagtgtatct gcacctgcag caagcaactg ttctgcttgt tcaatagttt ctactgtggt
     4381 tatgagttcg atcataattt tctcctccta caacttcata atagtagcat agctctaaaa
     4441 actttccaat acttcttcta tttaaagtcg gatttttgaa aattatttca caaaaaatca
     4501 aaataaagtt cgatatattc tgtccaaata taaaaagaga ttgtgacaaa aatcttgtca
     4561 caacctctta atcaagtctt actattataa aaattcagct acacaggcca agtaactata
     4621 gggaacctac tgtttccttt ttaggtccgc gaacattcaa tgtgatctta ccttttgaag
     4681 cacctactgt tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc tcgctcaatc
     4741 gatcttcaat ttctttttga agtgcgcgac ggattggacg tgcaccatat tcaggatcaa
     4801 atccagcttt cccgatcaca tcgattgcag atggtgtgat tttcacttga atatcttgtt
     4861 ctttcaatcg tttgataatc gctttgctca tgatttggac gattgtatgg atttcatctt
     4921 tactcaatga atggaagacg acagtctcat cgattcgatt caagaattca ggtcggaaag
     4981 ctttctttaa ctcttctaag atacgttttt gcatggcttt gtgatcttta gtaacatctt
     5041 ggacattgaa tccgacattt ttttcttctc gaatttgtgt cgctccgata tttgaagtca
     5101 taatcatgat tgtatttcta aagtccactt ttctaccttt agaatctgtt aaatgaccat
     5161 catccaatac ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc acttcatcta
     5221 agagaatcac tgaatatggt ttttgacgaa ttttttctgt caactgtcca ccttcatcat
     5281 accctacgta tcctggagga gacccgatta atcggcttgt actgtatttt tccataaatt
     5341 ctgacatatc aacacggatc agggcatctt cacttccaaa catgacttct gaaagtgctt
     5401 tggccaattc tgtttttccg acaccagttg gtccaaggaa catgaatgaa ccaattggac
     5461 gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact gctttgacag
     5521 cctcatcttg accaacgaca cgttcatgca gaagtccttc cagttctaac aagcgttcgc
     5581 tttctttttt ctccaactgc tgtagaggga caccagtcca ctccgataca actgtagcaa
     5641 catcttcttc tgttacacga tcagaataac cttgctcctg ttttgcttca attgcagaaa
     5701 ctttttgcag ctttttacgt agtgcttttt cttgtctgcg taattgagca gcattttcaa
     5761 agtcttgttt ttggatcgct gcttcctttt cttgtactag ttcatcgatt tctagtttga
     5821 tcacagttga ttttgttaag tgatcggttt gatccaaacg gactttcgct gctgattcat
     5881 cgatcaaatc aattgcttta tcgggtagtt gacgagaagt gatataacga acagaaagat
     5941 tgactgctgc tcgtacagct tcatctgtga tctccactcc atggtgctct tcatatcttg
     6001 tacgtaatcc ttgaagaatc acttctgctt cttcaggagt tggctcatct acttgaatgc
     6061 gagcaaaacg tcgctctaat gcagaatctt tttcgatata tttttgatat tcatctaggg
     6121 tcgttgctcc gatcgtttgt aattctcctc gagctaacgc cggtttcaag atattcgaag
     6181 catcaattgc tccttctgct ccacctgcac caattaaggt atgcaattca tcaatgaata
     6241 agattacttg accatcatta tagatttcgt cgatgacctt tttcattcga tcttcaaatt
     6301 caccgcgata tttcgtacca gcaaccaaag atcccatatc aagcatcatc aggcgctttt
     6361 cttgcatgtc ttctggcact tcacctaaaa tgattttttg agctaaccct tcagcaatgg
     6421 ctgttttgcc tacaccaggt tcacccacca gtacagggtt attctttgtc cgtcggctaa
     6481 gaatctggat aagccgtttc acttctctcg aacgaccaac aactgggtcc agcttgtttt
     6541 cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt ccttgcggtg
     6601 tttgccgttt atcttgtaca tttacatttc tacgacgatt cattcctttt ccagcagaat
     6661 caccagctag ccccattttt tgtttcagaa cttgacgggc ttttgaaagg ctgataccta
     6721 agttgatcat gattcgagta gccatgatac tttcatcttt taataagctc atcaaaatat
     6781 gttctgttcc aactttttga gaaccagttt tcttcacttc gctgctagct aaagcaaaaa
     6841 cttgtttcat tcttggtgaa tatggaagaa agactccttt agggtaagaa cggataccac
     6901 cataaccaat cagatgttca atttcttcat aaatttccgt ttcattcaaa tccatttcac
     6961 gtaatacttt tccagcaatt ccattttgct cgaccactaa ggctagtaaa acatgttctg
     7021 aactaactga ttgatgtttc aaccgttttg cttcttcttg tgcaatttct aatactgctt
     7081 gggctctttc agtaaaaagt tcttccatct aatctcactt cccatcttca taacttaaac
     7141 gttccagaaa tgctcggaga atccgagcac gtgtatgatc ttcatattca ttgaaattca
     7201 atgtgttttt tgcaattgca ctcaacatca agtttccttc tttttttgta attatttggt
     7261 cttcataaag tttttgaata atagcaaaag catttttctc actgattgta tcatcaaata
     7321 actgatttat ctgctcaagt aattgttttt tatctgaaat ccttactttt gcaattcgta
     7381 tataaccacc accaccacgt ttgctttcta ctagataacc acgttggatg gtaaaacggg
     7441 tattaatcac atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc atttcaatcc
     7501 ttcgaatctc gatcatttca ctattttcca gaattttctt tatatatgat tcaattaaat
     7561 ccgaggtatt ctgatgtgcc atcttattca ttccctttga ctaatattga ccttaattat
     7621 aacgaatttg ataatagaaa gaaagaagaa cgctttttgt taaataaatc ctttagaatc
     7681 aacaaaaaaa aatacggaat atactattcc gtatcgacgc gcccaagagg agtcgaaccc
     7741 ctaacctttt gatccgtagt caaacactct atccagttga gctatgggcg catattcaat
     7801 ttaaaaaaat tataaatcat ttgaaaacaa aaagcaagat aaaaaaaatc gggaagacag
     7861 gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc taccaagctg agctacttcc
     7921 cgtttaaaaa ataaaaaaga c
//
LOCUS       DACEZN010000125         7847 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEZN010000125 DACEZN010000000
VERSION     DACEZN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.125"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..866
                     /locus_tag="IXH60_002177"
     CDS             <1..866
                     /locus_tag="IXH60_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1991076.1"
                     /translation="NKHKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIR
                     NRKAYGTRKLKQCLANLGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTAN
                     ILDGTFLQEKSLEAIVTDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKE
                     AFAKIPYSLNNVQLFHTDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYK
                     SVKVEFVHQYRFETLANLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDN
                     ELGCDTGGEAP"
     gene            1146..2126
                     /locus_tag="IXH60_002178"
     CDS             1146..2126
                     /locus_tag="IXH60_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1991077.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            2248..3666
                     /locus_tag="IXH60_002179"
     CDS             2248..3666
                     /locus_tag="IXH60_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1991078.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            3737..5206
                     /locus_tag="IXH60_002180"
     CDS             3737..5206
                     /locus_tag="IXH60_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1991079.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            5216..5683
                     /locus_tag="IXH60_002181"
     CDS             5216..5683
                     /locus_tag="IXH60_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1991080.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            5719..6591
                     /locus_tag="IXH60_002182"
     CDS             5719..6591
                     /locus_tag="IXH60_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1991081.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            6673..>7847
                     /locus_tag="IXH60_002183"
     CDS             6673..>7847
                     /locus_tag="IXH60_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1991082.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLES"
BASE COUNT         2659 a         1166 c         1610 g         2412 t
ORIGIN      
        1 ccaataagca caaatactca atatcagcga tgtgtaaagt gttaaacatc tcttgccaaa
       61 cctattatta tcaagccaaa ccagttgaag atgaatcaga actggaagaa ctcgttcaag
      121 aagaatttat tcgcaatcga aaagcttatg gtaccaggaa gctgaagcag tgcttggcca
      181 atctaggtct tcaagtgagt caacgcagaa tcggccgtat gatgaaacag cgtggcttgc
      241 gttcaacata cacattggct catttcaaat tccattcttc tacttgtaac gaagccaaga
      301 ccgccaatat tctagacgga accttcctac aggaaaagtc attagaagcg attgtaaccg
      361 accttaccta tgttcgtgtt gggaagaaat ggcagtatat ctgcctgatt cttgatttgt
      421 ttaatcgaga aatcatcggc tattcatgcg gggagaagaa agatgcgacg ttagtcaagg
      481 aagcttttgc aaagattccg tactcgttga ataatgtaca actttttcac acggatcgtg
      541 gaaaagaatt cgataaccag acgattgacg aaattttatg ggcctttaat atcacgcgtt
      601 ccttaagcaa aaaaggctgc ccctatgata acgccgttgc agaatccacc tataaatctg
      661 tcaaagtaga attcgttcat caataccgct ttgagacatt ggctaatttg cgattagagc
      721 tatttgatta cgtccattgg tggaatacca tccggctgca cggcacgtta ggctatgaga
      781 caccgatcag ttttcgacag cagagattgg cgcagcgaat ccttgataat gagctcggat
      841 gtgataccgg tggcgaggca ccttaattat ttgaatgagc ttctgcctta gagaatcaga
      901 ttcgaggagg ctcgttgtca agggcagtcg gaacaccagc agcaagctta cgcaatacct
      961 tataattttt gtcaaaaaaa gtgttgccat tccacatata tctttaaaca gctattgcca
     1021 gataaaaata aatgatatta tatatataaa tacttatccg aaaaggggaa tgagagagtc
     1081 aaggttcttg gaatgtcgaa tagtcgagta ggtagatttt ctatctacaa ccttgttgtt
     1141 taaaaatgaa aaaaattgga attagagaag ttgcgaaaga ggcaaatgtt tctcctacga
     1201 ctgtgtcgag ggtattgaat gatcgtggat atttgagtga ggaaacaaaa agaaaagttt
     1261 ttgatgccat gaaaaaatta gaatactatc ctaatgaact ggcgagagca ctttttaaaa
     1321 acgaaacata taccgtaggt ttgatttttc ctacaattac taatccattt cacgctgagt
     1381 taattcaaga tatcgagttc gagctttcca gcaggggata taaagtgttg ctgtgcaata
     1441 gcttaaacaa tccaacaaaa gaaaaagagt acttgacgat gttgagaaag aatcaggttg
     1501 atggaattat agtaggaacg cacaataaag atattgcgga ttatgatatt ccagggctac
     1561 ctattgtagc gattgataga agtcttggtg aaaatacagt tacagtatct tgtgataatt
     1621 acaggggtgg ggaattagct actcaactac ttgtaaatca tggctgtcag aatatactgt
     1681 gtattcgggg agatagtaag ataaagctac ccgcgaatag taggacttta gcatataaag
     1741 atgttttgaa taaattcatg ctggaaccgt tagtcctaga agtaccattt gtaaaagaaa
     1801 tcaatgaaaa aaatgaaata attaagacat atctggaaac tcatccaaat attgacgggg
     1861 tttttgcagg agatgattta ttggcttctt tggcgattaa caacttagga agttcgggca
     1921 aaagagttcc aaaagaggtt aaagtgattg gatttgatgg agctcaacaa acattgattt
     1981 ataatccaga attatcaact attcaacagc ctatagatca aatatctaaa gtagcagtaa
     2041 aaaaattatt gaatatgatt aagggcgaaa aggagacgga tgaactttca cttccagtaa
     2101 agctcataaa aaaaaataca gcataagtta aatcggcgaa aaattccttc gtcgatttaa
     2161 aaatattttt atgaaacggg ttgacatatg aaacgggttg acatatatta gttgtgaaga
     2221 taaaaacctt ttcactggag gaagaaaatg gagaaattat taattaaaag caataatcat
     2281 caactttact accaacctaa ggatgtttgg gtcggagatg ttatgcctta cgggaaagaa
     2341 ggaaaattct ttatttatca tcaacgtgat actagaaatc cagttccttt tggagaaccg
     2401 tttggctggg ctttagcaac tacgaaagat tttgttcatt ttgaagactt tggggaatct
     2461 cttttaagag gggatgacaa tgagagagac cagtttatct acgctggttc tgtatttgag
     2521 gcaaatggaa aggttcacgc attttataca gggtataatc gtgaattctt agcgcaggga
     2581 aagacatctc aagtgttgct tcatgctgag agtgatgatt ttgttcattg ggaaaaatca
     2641 gaaacagcat tggagttaga accacaagaa gggtatgata ttcgaaactg gagagatcca
     2701 tttgtcgttt gggatgatga aaaagaagaa tatttgctaa ttttaggggc gcgaaagggt
     2761 caggataaac atctacaaac cggacgttta gtggaattta catcaaaaga tttaactaat
     2821 tgggaattca aaggtgattt ttgggctccg ggattgtata ccatgtttga aatgccagaa
     2881 cttttcaaga ttggtgactg gtggtatctt gtctattcag aatactcaga tggaaataaa
     2941 atactctatc gaatgagtca aagtttggaa ggtccttgga ttaagcctaa agatgaagct
     3001 tttgatggac gggcttatta tgcaggtaga acagcttatg atggaaaacg tcgagtccta
     3061 tttggatggg taccaacaaa agatcaagaa aatgataaaa acaattattt atggggaggt
     3121 actttggttc ctcatgaagt ctatcaaaaa gaggattttt cacttggggt aaaacccata
     3181 gacgaagtgt ggaatgcatt cataaattta catgatattg ccgatgtaga gatagaaaca
     3241 gcagacacaa gagaagaagt aacgcttgtc aaaaatagtg gaactttgtt taatttagaa
     3301 acaacaattt catttgatga gaatcttagt gacttttcac tgagattata taaaaacctt
     3361 gagacagatg aatcttacga atatcgcttc aatttagatg aggggcgtct tacatttgat
     3421 aaaaaccctt gttacccttg gtttcaatat atggataaag gacttattag acctatatct
     3481 ttaaagccta accaatctta taaactaaaa gtgattgttg atgacaatat aatgacactt
     3541 tatattgatg gaatcgctct taacgctcgt gtacatggtt gttttggtca agcaattact
     3601 attacagcaa caaatggatc aatgactgca ataaatacca agttttcaaa tgaaataaag
     3661 aaataataaa cgtcaatcat gtggcaggtc tgaaaatcaa acatgacctg tcacatttaa
     3721 aagggaggat aaatgaatgg actacaaaaa aattgccgaa gatgtattgg aaaacgttgg
     3781 aggaaaagag aatatctcaa gcgctacaca ctgcattact cgtttacgtt taattcttaa
     3841 agacaaaagc aaagcgaata tggaaatgct tgaggaaata gaaggtgcaa aaggtgttct
     3901 tttcaatagt ggacagttac aagtaatttt cggaacaggt ataattgaaa aggtttacaa
     3961 agagttcgtt gaattgacag gagctaagga agtatccgtt tctgaaatga aagacgaagg
     4021 agtcaataat tcaggaaata gacttcaacg agcttttaaa gttttctcgg atatctttat
     4081 tccgatcatt ccagctttta ttggggcagc aatggtttta ggaattcgtt cgttgattac
     4141 cactcctgga atgtttggaa tggatggctc attcgctgat cacaatgtat tcctaaatga
     4201 tttatccaaa tttctgggta ttattgcaac cacatttgat tatttaccag ttttagttat
     4261 gtattctgca acaaaacgtt ttggcggaaa ccctatttta ggattacttg taggatttgt
     4321 tatgattcat ccaggactag ccaatcggaa tgatttcgtc atgggaacta taaagcctga
     4381 ttattggcat ttatttggat taagcattcc agcagttgcg tttcaagggg gtgtttttcc
     4441 agcaattttg acttcttggt ttttagcaaa agttgagaaa attacaaata aatattctcc
     4501 tcaagtactt gcttacattt tagtcccaac tgtgacaatt gttcttgcca actttgcact
     4561 atttttagtg tttggtccat tggggaattt tatcggtaat gttttaggtg gaatcattga
     4621 tttcctatat atgaagatgg gtgcctttgg tgcttttgtt tttgcaggcg cgttacaacc
     4681 attagttgta acaggaacac aacatgctat tcaaggtatt gaggcaaact tagtcgcaac
     4741 aactggattc aattatatcc aacctatttg gtcagtttca attattgccc aaggtggcgg
     4801 ttgtattggt atgtatttac tctttaagaa aaaatctaaa gatagagata ttgcgatttc
     4861 aagctttatt ccaacactgg taggtattac tgaaccggct atctttgctg ttaatttgaa
     4921 atattcaatc gttccttttg tttgctcgtt tattggtgct ggttttggtg gagcatttat
     4981 gaaaattttt gatgtaaaag ccattggaca aggattgact gtattgccag gtctgactat
     5041 tgctaatcca gttcttcctt atatatttgg taatctcata gcattcattt taccaatcat
     5101 ttttattctc gcttatgata aatataaagt gtttatccct gaagctaaac accgtaaatt
     5161 tgcagaaaaa actgactcaa aaaaattgcc agttcaagta aagtgagatg agagcatgtt
     5221 aaattttttt agaaaaaatg aaataagcgt tgtagcacct gcaaccggtg tttttgaaaa
     5281 actggagaaa ttaagtgatc cagtcttttc aaaaggaatg atgggacaag gatttgcaat
     5341 tgatgcaaaa aacgaaacga ttgtatctcc aatcgatggt tttgtttcaa gtatttttcc
     5401 tacaaaacat gccttaggaa taaaaacaaa atcaggaatt gagatacttg ttcatgtagg
     5461 aatagataca gttgagctaa atggtgaggg atttgatatt aaagttcaag aaggacaaaa
     5521 agttaaggct ggggatattc ttatgaccat taattttgta gtttttgaaa agaatcacaa
     5581 gactaaagat gtcattgttg tttttccaga ctaccaagac gaaattttca ttccgaaact
     5641 tgaagaagta aaacaaggac aagaattgtt tgttcttggc taatgataag taattaataa
     5701 tagaatcgag gaaaatgtat ggtttttggg gctattgaag ctggtggaac aaaatttgtt
     5761 tgtgccattg gagataaaga tttgaacata tttgaaaaag tcagctttcc cacgactaca
     5821 cccaaagaaa caatgaattt agttttagat ttttttaatc atcataaaga tgatttagaa
     5881 ggtatcggag tgggatcgtt cggtcctatt gatattcatt gtgaatctaa aacgtatggc
     5941 ttcattacat caacgccaaa acttgcttgg caggattttg attttattgg aagtttaaaa
     6001 aagcaactta atattccaac tgcttggacg actgatgtaa atgcctcttg ctatggtgaa
     6061 tatgtttctg gaaatggcag aggcctttca agtgttgttt actataccgt aggaacgggc
     6121 ataggaggtg gcgctatcca aaacggaaac ttcattgaag gttttagtca tccagaaatg
     6181 ggacatatgc ttgtaaaacg acacaataaa gacaactttt ctgggacatg tccattccac
     6241 ggagactgtt tagagggctt agctgctggt ccggcaatcg aaagaagatc agggaaaaag
     6301 ggacaagaag tgactgaaaa tgacgttact tgggaaatag aagcttatta cattgctcag
     6361 tgtgcatata atacaacatt gatgttatca cctgatgtca ttatatttgg tggcggagta
     6421 atgaaacaaa aacacttaaa aaagaaagta caagataagt ttattgagtt gatcaacgga
     6481 tatgtcgcta ttcccgatgt tgacaattat attatgacac caaaattgtt agataattct
     6541 ggtattgtag gttgtttagc tttagcaaga gacgttaaag aaaaatctta atttggaaaa
     6601 aagatcgtct cgactatttg tttggttgat gcgatctttt ttttcaatta gtaattgaag
     6661 ggacgcctaa aagtgaaaaa aaaaatgaaa gatgaagtat tagagtatat caaaaataat
     6721 ttaaagtatt acgatttttc tgctcaagat attgcaatga aattttgtat taaaagaaat
     6781 gtcgcttccc actatttgaa tcagttattt tcagatggaa aattactaaa gaatgattct
     6841 gttagaccag taatgttcaa gtataatcaa caaaagccaa aagactgctt ttctaagttt
     6901 attggagcag acatatcttt gaaatccact atagataaat gcaaagctac tgtaatgtat
     6961 ccccctaatg gcctcccatt aataataaaa ggaaacagtg gcgttggaaa aagtttttta
     7021 gcaagtttga tctatcagta tgctcttgat agaaaagtaa ttcataatga cgcaaaattt
     7081 gttgttgtga attgtgcaga ttatgcaaat aatccagagc tattgtcagc agttttattt
     7141 ggatataaaa aaggagcctt tactggggct gaaaaagata ctgcagggat gctatcttct
     7201 gctaatggag gatacctttt tttagatgag gttcacaatc taagtgcaga aaaccaagag
     7261 aaattgtttc ttcttatgga ttctcaaaag tatcgtaaac ttggtgaaag tgtgcaatgg
     7321 gagtatgcaa atgttcgcct tatcttagcc acaacagaag ataaaaatac ctctttgtta
     7381 gcaactttta gaagacgcat cccagctgaa atcactctgc cagattacaa taatagaagt
     7441 acatctgaaa aaattcaatt attatttgaa tttcttaaag atgaagcaat tacaattgat
     7501 tcaaaaatat tttgttcgat tgaacttttt actgatcttt tatcgaaaac atttgaagga
     7561 aatgttggag aattgaggaa tgaaatcaag cttctttgtg cagaaggtta tttaggaaat
     7621 aagagaaagt cttcaattta tttaggggac aaactagaca gcggtttttg gatagatcca
     7681 gaaattagta tagatttaaa aaaaatgttt cttaaatcac tttctgaaat tgatttgatt
     7741 gaactttttc aaaatcacat ttcaattgaa gaaagcatga gtcaattaag agatagaata
     7801 cttaaagagt gtgcaggttc ttctgagtat aattatttag aaagtga
//
LOCUS       DACEZN010000126         7834 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEZN010000126 DACEZN010000000
VERSION     DACEZN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.126"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            167..2494
                     /locus_tag="IXH60_002184"
     CDS             167..2494
                     /locus_tag="IXH60_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1991083.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2620..2949
                     /locus_tag="IXH60_002185"
     CDS             2620..2949
                     /locus_tag="IXH60_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1991084.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2989..3993
                     /locus_tag="IXH60_002186"
     CDS             2989..3993
                     /locus_tag="IXH60_002186"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1991085.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3990..4436
                     /locus_tag="IXH60_002187"
     CDS             3990..4436
                     /locus_tag="IXH60_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1991086.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4586..5401
                     /locus_tag="IXH60_002188"
     CDS             4586..5401
                     /locus_tag="IXH60_002188"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1991087.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5607..6779
                     /locus_tag="IXH60_002189"
     CDS             5607..6779
                     /locus_tag="IXH60_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1991088.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6941..7435)
                     /locus_tag="IXH60_002190"
     CDS             complement(6941..7435)
                     /locus_tag="IXH60_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991089.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7432..7626)
                     /locus_tag="IXH60_002191"
     CDS             complement(7432..7626)
                     /locus_tag="IXH60_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991090.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2661 a         1462 c         1545 g         2166 t
ORIGIN      
        1 tttttattta aaaataaaat ctttgtatta aaggacaaga ctaaaataac cttagaataa
       61 aaagagaaaa aaagccccaa actttctata catcaataaa ataataaaaa ttaaatttag
      121 aataacgtgt aaaggattat ttgatgattt tttattgaga ggagggatga aaatgattaa
      181 gaaaactcaa aataaaatcc aacgtatatg tcaaaaacat tttgtttcta gactcaaagc
      241 tgtatctaaa agaaaatact tacatcccat tatgatttcg atcatcttga tttttacttt
      301 cttagggagc ttatcaagta tttatgtaaa cgtttataca gaaacgtcaa attttgctaa
      361 ccaaacgaca gaggagacaa atagtccacc ctattctgaa atacagtcca tagaaagcac
      421 caatcagtct agcaacaacg agacaacctc aaccacatcc tcgactcaac tgattgatcc
      481 gacagaaaaa gaaatcacag cagcaaaagc agcaggggag aaagcttatt cagaaacagg
      541 tagaagccag acatttagta ctgttgcggc cactgcgaat ttcaacggac ataccatacc
      601 agatggatat acagtgatat atgccaaaga tggagtcatt gttgcggtta acggatcgat
      661 gagtgcggat aaaactgcgt tacaaggcgt aaccaaagca atcgctttta ttgataaccg
      721 tccagatatc cataatctgt ttattggctc cacagtaaat ggtactacta tcacattccc
      781 aaaaagtatc tatatggggg tggctaccgc tggaaatacg atcgattcag ttacacccaa
      841 tgcggataaa agttattctt ttacaccctc aacatgggga gggcttcctg ccggaggagg
      901 tatgagtact ttttcaatcg gtatacagtt agcaaatgat attccattca cggggtactc
      961 gatcaaaaat gggcagatcg ttgatggtaa gggaaatgtt tcaaattttc caacctatca
     1021 agcagcaacg gttcaggtca atgcgaatcg gacaggaact ttttcttata cagtcaatgg
     1081 tggagcagca aaaatcggaa cgtacgggac tacattatct ggaatccatg tgggtgacaa
     1141 agtggttatc actccaacag cagtaacggg ctattctttt gcacaaacaa acggttcttt
     1201 cacagcccta gaaagtaacg tcgatacggt gacctataca ggcaaacaaa caccaatcac
     1261 tattaagtat caagactatt ttggacagat gattgctcct tctaaaataa tgaatcttac
     1321 ttacggaagt gctgcacaag acctgacgac taatacgcct actatcaacg gatatacctt
     1381 tacagctgtt tcagcgatag aaacaaagaa ccaatcagca atgagtgttt tagctagttt
     1441 agatacaaat ggaaatgtaa tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat
     1501 cctatattat aagacaaatg caacagtatc aatcaatgca aatggcagta agtactatga
     1561 tggtctttct gttgtaccga tcgttaaata tgcctttaat gataaaacag aatcaacatc
     1621 ccttttaaac aatgatctca gtattgatca aatcacatgg aatccaactg atttcaaagc
     1681 gatgaatgaa aagggagttg aagtaactgc gcctactgaa attggaacct atacgtcttg
     1741 gcaattgaca aaaagcggac tagctaaact agctgccaga acaaactatt tatttactat
     1801 cgtgcagaca tctgatgtct atacgattaa gcaaatcagc ggtgcggtag agctcagtgg
     1861 cagtaagaca tatgatggaa aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg
     1921 tgtcactagt tcgcttactc cagtacaagt ggcacttagc agtatggatt acacagcaga
     1981 tgcacaatca gcaaaaaata tggttaatgc tggcagctat acaattaaac taacgaatag
     2041 cggaatagac aaagtcaaag cagcaaatcc caagttgtca tttactgata tagccaacac
     2101 atcaggaact tataccatct ataaagctaa cgcagtgatt actgtcgatg acgctgcctt
     2161 taattatgat gcccaaagtc actcgatttc ggttggaaat ctccacgtta ccggtgttgt
     2221 cttaggcgaa tcattggatt ataccctaac agttaacagc cggacagata ttggatcaca
     2281 aatagtaacg attagtttag gcgaaggaag tgtaaacaat aattatacga ttatcgcaac
     2341 agataccgct aagttgacga tcaatccgac tctcttgctt ccttcgacag gtgggatagg
     2401 tctactgccc tttgtctttt taggactgat ctttattttt tctggcttct tttattttat
     2461 ccatcgtaag aaaaaagcag gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat
     2521 atatttgctg ttctcttttt tatcctactg gtctttttta taggttctcc gactgtgtca
     2581 gctagagaat cacctattac atttactttg cataatcgaa tgttcgaaag cgataagatg
     2641 ccaagtatca gtcaaaacga cggaataaat atcccggata actttcaagg agtaaaaggg
     2701 ttgaacggcg ttactttcgc tatatatgat gtctcggatg aattttacaa gctgcggtca
     2761 gaaggaagta gtgtggaaga cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa
     2821 atactggcag aaaatgtgac aaaaacagta gacgaggaag agggcattgc ttcttttagt
     2881 gcatcggaca aagatgagca aggtcgtgac gctgtttacc gatttacgga aataaagact
     2941 tcagattaag taaaagaaaa atcagcacca tttgtcgttg tgcttcctgt gacagattca
     3001 tctaatcata aattgacgac catccacttg tatccaaaga gcgaacaaaa aatacctgct
     3061 gcactcacat taacaaaaac agtagagaat aagcagacag attttgcaga tggggataaa
     3121 gtcccttatc tcatcaccgt aacaattcct gagaacatca acgagatagg aacctatacg
     3181 atcaaagata cagctgatcc tcagttatgg ctagaaccag aaacgatcga ttttttgatt
     3241 ggtggagaca agattcatac atttcacaca caaaaaataa aacatggttt tgtcttatca
     3301 gattctggaa aagatcttag ttcgtttgct ggaaaaaaat taacgatccg ttaccaaatg
     3361 acattgaaag aaaacagtga aaaaacgaca tttgataacg atgtacgttt gacgactgac
     3421 aatcaaacag tagaaacaca tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg
     3481 gatcggcaaa cgaaacaaaa actagcagat gctcaatttc tagtcaaaaa aaatgaccaa
     3541 tacttagcgc aagaaaacgg gataaatcat tgggtagcca aagatgattc aagagcaacg
     3601 atttttactt ccagtaaaga tggaacattt tctgtccatg gattgtcttt tggaagttac
     3661 gaactagtgg agattaaacc gcctaaagga tatatattga gtcaatcaac cattctcttt
     3721 gaagttgaaa aaggcagtta tgatccgctt catcctatac cattggaaat catcaacgaa
     3781 ccaaaagcaa atccgacgaa taatggaaaa acgccagttc agatgcagac aaatggtcca
     3841 tcaaatgaaa aaaatggagg aagctttcca aaaacaagcg aagagatgct tggaggtttc
     3901 tcaattttag ggttattgct agtcctttca acagggacag cttggtttta taaaaaacaa
     3961 caaaaaggaa acaataggag ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt
     4021 ttttaggaac tgctatcatg ctgcttcctt tcgttctcgg cctaggaact gcagaggttt
     4081 ctgcagccgt atcacctaca ccagaaaatg ttacggttaa tttgcataaa ttgaaattca
     4141 cttcagcacc agaaaatcaa ataaacaacg gaacggaatt gacatttccg aatagcgaac
     4201 cgttgaacgg cgttgaattc aacgtatatg acatcactgc tacgtactat ccaagtaaag
     4261 acacagcagt tccagcagac gctacaccct tcgcaagtgt aacgacttct ggtgaaggat
     4321 tagctaacct gactcttcca ggtaaatctg atgggaaaga tgctgtatac gtctttgtag
     4381 aaacaccaaa gcccggtgtc gaaacgtcgc caaatatcgt gttaagctta ccctagaccc
     4441 tgacaaacct ttagacacga ttcacctata tgctaaaaat gtcatcaaag aaagcaatat
     4501 caaaatccag aaagctggga acttttcagg ggtagataag ttagcagggg cgcaattcca
     4561 gatcaaaaat tcagagaatc aattcgtgac cggttttgat gaaaacactg gtgtcgctct
     4621 ctatgatcct gctgagtcag cgttgacatt cacaactgat actgaaggat ccatcagtgt
     4681 caatggatta atagacggca attatacttt agttgaaaca aaagcaccag atggttatca
     4741 attaaccaat ccacaaaaag aaactacttt tatagtaaaa aacggaaaat tgcaacaaac
     4801 aaccggtatc atccatggcg cagatgaagc caatcttggg aatccaaacg gtgtagtcaa
     4861 tcgggaaaat acaactacgg ataacacgat tactgtagaa aatgtccaaa actacggtga
     4921 tttccacttc atcaaacaag atgtgaacac gcaagaaaaa ttagccgatg cagaattttc
     4981 tattctaaga gcaaaagatc caactgttaa attaatgaaa aaaatagcaa accttgcctc
     5041 aggagaatac ccatatgctt ggtcagatga agtcacaggt tctgactggg aagaagtgaa
     5101 attgatttct gattcagaag ggaaattttc aatcgaaggg ctgaaacaag gggcatactg
     5161 ccttcaagaa acaaaagcac cagaaggcta cgcattacct aagaatgaag ctgcgtacac
     5221 tgaatttaca gtagatgatg atccaacaac ttctgatgaa acaacgatcg ataaccaacc
     5281 taaaggaatc ttgccttcaa ctggtggtat ggggatcatc gtattcatct tggtaggtac
     5341 tgctttagtt ggaggagcag tgatctattt caaaaaacgc catcaagaaa cagaagctta
     5401 aactattgga aacaaaaatc atacgataaa taaacgatag tgattcaaat ggggagaaag
     5461 gttgtgacaa gtggtcggtc tcttggtatt cgctcaacaa acggcaaaat aggaggaact
     5521 attttgccgt ttgcttcgct aatactcagg tcaaggcgct gatttcacaa cctcttcata
     5581 tacatattag aaaataggtg gacatgatga ataaaagcag aataagaata ctggacatct
     5641 tcatggcaat cattttagtc gttgggatcg ggatcttttc ttatcctttt gtagaagatt
     5701 cactgaatga ttttcttgcc cagcaaatga tcatccatta tcaaaagcaa gcatcgaata
     5761 agaattcaga ggaaataaaa aaacagcagg aaaaaatgac aaagaaaaac caacagttgg
     5821 cagagcaaaa cgtatcacca gggataggca gtttcaacca agcagttgat gcaaaagcat
     5881 taaaagattt acctagtaat gcctttttta tggaacatat gcttggagtc atcgaaatcc
     5941 caaaaatcaa tgtcagctta cctatattcg atcagacaac ggaaatattt ttgcaaaaag
     6001 gaacgtcatt attggaagaa agttcttatc cgactggcgg gaaaaacacc catgccgttc
     6061 tttctgggca tcgtggactc ccagaagcaa aattatttac agatttgcct aagttgaaaa
     6121 aaggagacca gtttttcatc cagatcaatg gaaagacatt ggcgtatcaa gtagaaaaaa
     6181 tccaagtggt cttacctgat gaagtagatt ctttgggtat ccaaaaaggc agagatctcg
     6241 tgactttgct tacctgtact ccttatatgg tcaatacaca tcgactgctt gtgacggggc
     6301 atcgtattcc ttatcaagca aaagaggcaa aaaaagcgat ccaaggaatt gatcaatgga
     6361 aaaaatggaa attttttatt tggtttatcg gtatactact tggatcaatc ggcttagttt
     6421 ggctactcat cgcttattta gattctttgg ccatcgctaa gcgaaactat cctttatctt
     6481 tttatgtaaa gaacacaaac ggacgaccaa tcgaaggaat ggtcttttct gtaaaaacgc
     6541 tcaacggcaa acactatatt actagagaaa aagttccatt tgtgaaagct tctgatgaat
     6601 acggactagt gagattttct gatttaaaag gcaggaacta tcgcttgcaa catgaggagc
     6661 tcctgcttaa aatccatgta aaacataaac attcgaaaca gttttcaatg aaattgaaaa
     6721 aaggaagata taaactgcga aaagaaaaag aggtgtacta tttgattgag aaagaatgaa
     6781 tgaagatgtc caaaaaagca gagggaacag actaaatgaa aaaagagaga agctgacgaa
     6841 atggacgtgt aaactgttat ttttggttta ctatctggaa cgccatcttc tattttttta
     6901 tgtttcagaa gaattttatt attatagtat aacttttgaa ttagtttcca taacatttgg
     6961 agcaaggggt taaaccacga gcttgagctt cgcttaaagt ggcaggggta tacgtaccat
     7021 ttccacattt atgtgtatgg tattttgaac cggtcggtgt gacatagaca gtttgttcat
     7081 tattttgctc aggagctgcg gcttgtgcct gctgagaggc ctgttcagct gctgattgct
     7141 gtcgcgcctg ttctgcttgt tgctgtgcga ttgcttcttg cctcgcctgt tctgcttgtt
     7201 gctgtgcgat tgcttcttgt tgcgcttgtt ctgcttgttg tcgtgcaatt tcttcttgct
     7261 ttttccgttc ttcttctgct tcttttgctt tgattgtgga atcaattgca gcaagacggc
     7321 ttgtcaactc ttgtttgttt tcaggtagcg cttggatagc tgctgaagca gttgcatagt
     7381 tttcttttgt aggatttgct tcagcttgtg acaaagcaga ttccgcttct gtcacttttt
     7441 ttctagtgca gctttttccg ctttttgttt ctcttctttt ttcttttcaa tttgttcttg
     7501 ttctttcgat gaatctgccg tactttcgga gatcgtggta ctgctgctgg aagcctgatc
     7561 tgaacttgtg ctttgagtag tagacgtagt agcaccacac ccactcaaca aacttcctgc
     7621 gaccaaaaac aatgcaacta attttttcgc taaatttcct ccttaagtaa aaaacaaccg
     7681 ttttcaataa atgtcctttt caattgttta cttacttgtt ataataattg tgttatgaat
     7741 agtgattgag gaaacttaat ctaatcccgt gttgatacac gggatttttt gctatagttc
     7801 tgttattaag tatgcaataa taaaatatgt acgt
//
LOCUS       DACEZN010000127         7706 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEZN010000127 DACEZN010000000
VERSION     DACEZN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.127"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1364
                     /locus_tag="IXH60_002192"
     CDS             <1..1364
                     /locus_tag="IXH60_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1991091.1"
                     /translation="NLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEK
                     EHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLS
                     SFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITN
                     WHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHP
                     IVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIP
                     KGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATID
                     PVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLE
                     GARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHF
                     SRN"
     gene            complement(1536..2108)
                     /locus_tag="IXH60_002193"
     CDS             complement(1536..2108)
                     /locus_tag="IXH60_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1991092.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(2115..2777)
                     /gene="rpe"
                     /locus_tag="IXH60_002194"
     CDS             complement(2115..2777)
                     /gene="rpe"
                     /locus_tag="IXH60_002194"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1991093.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(2793..3308)
                     /locus_tag="IXH60_002195"
     CDS             complement(2793..3308)
                     /locus_tag="IXH60_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1991094.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(3310..3594)
                     /locus_tag="IXH60_002196"
     CDS             complement(3310..3594)
                     /locus_tag="IXH60_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1991095.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(3628..4956)
                     /locus_tag="IXH60_002197"
     CDS             complement(3628..4956)
                     /locus_tag="IXH60_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1991096.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(4970..5425)
                     /locus_tag="IXH60_002198"
     CDS             complement(4970..5425)
                     /locus_tag="IXH60_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1991097.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(5428..7374)
                     /locus_tag="IXH60_002199"
     CDS             complement(5428..7374)
                     /locus_tag="IXH60_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1991098.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2532 a         1466 c         1184 g         2524 t
ORIGIN      
        1 tgaatctttt tagtgatgaa gaaatttctg taagtgttcc agaagcagct gccaccatta
       61 ttccagtaaa aggacatcaa cgagttgtag gaacgaaagc agacaaaatc aaacacttac
      121 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
      181 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      241 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      301 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      361 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      421 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      481 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      541 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      601 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      661 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      721 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      781 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      841 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      901 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      961 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
     1021 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
     1081 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1141 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1201 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1261 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1321 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1381 cgattctatt ctataggtcg ggttatttga cgtacaggta ctgttctatg gggcgcttac
     1441 gaattattaa gttatgtcta atcaaataaa aaaaccattc tcatatttgc tgaataatta
     1501 gcatttagag aatggttttt tatctttaaa atctactact caaaatctgc atgtcgagca
     1561 aacatcgttt ctgagctttc acctgtttta tccattaaag ataacactaa actatcaaag
     1621 gtgagaaatg ctaattgttc aaaacttgaa cccataggtt gtgaaaaagt atcttcttct
     1681 cgttgatttt catctttggt aatacctggt aatacaagaa cagaatctgc taattgacca
     1741 atcgacgagt cttttttcat ggtaactaaa gcaatgctta caccactttg atttgctttg
     1801 gccgctaaat tttttaaact acctgtttca cctgagccag aacagataat taataaatct
     1861 ccaggttgcg aatgtggtga actaatttca ccgacaaggc tgactgaaaa acctaaatgc
     1921 attaagcgat tcgtaaatgc ttgcatggca attccagagc gtcctgcacc agctagaaag
     1981 atgtgtttag cttgaacaat tttctcacta aattgttcta actctacaga attaatacgt
     2041 tttgagttat tttgtaattc taagataatc gcttctaaat agtttttatt ttggtcttta
     2101 ttggtcatga tcttttactt cacttcctct aatgattgat ttagtttatc aatttgttct
     2161 tgcggggtat ttttcccatt gaagatgaaa ccgcctgata caaaaatatc cgcccctgct
     2221 ttagaacaaa ctttaatcgt ttgattatca atcttgccat ctacttcaat atcatacgta
     2281 taatcatttt gtttgcgtat tacatctaac tgttcgattt taactactgt ctcttgaata
     2341 aaatgcttat tctctgttcc tggattgatt gtcattacta atacttgatc gactaaagat
     2401 aatactggtt caataaaact gactggtgtt gcaggattaa aagtaatccc tacttttgct
     2461 cctgcttcct tgactaactg aatgaccttc attgcgtgtt tggtagcttc aatgtggaag
     2521 gtaacaatat ctgctcctgc ttctacaaat cttgtgacaa ttttttctgg tgtatcaatc
     2581 atcaaatgaa catcaaacac aacgtctgtt agtggacgaa tatctttaat aacacttggt
     2641 ccaaaagcca tatctggtac aaaacagcca tccattacat caatatgcaa ataatcaaca
     2701 ttcttttctt ttaagatttc aaataactct ttaacttctg cgataggtgc atttaacaat
     2761 gatggagcaa ttgtcattct ctattccccc gtttattctc ccataatcat gagattgatt
     2821 gttctagttc gctctcttag ttgatcccaa tcgacaaaat agacacggcc catttctcct
     2881 aataaaagtt taccttcttg aacaattaaa gtcattgagt tggttccata aatagatgaa
     2941 cgtaaatgtg catcggtatt tagcatgacc catttttctg cagggtaatt gggatcagat
     3001 aatcttgttc caaaatcaat gtgttttggc cccggactgt tgtattgatt ttcactcgtc
     3061 atttttggaa taatcgtttc cattacattg ttgatgtcta catgtaaata atcatcacca
     3121 aaaaagtttg tgtcatgcat cgtttcttca aaaaagagtg agcaagttgt atgcgggctc
     3181 gaaattacta acattccatt ctcaatacct gctgtatcaa ctgtatctaa aagttctttt
     3241 cgaatatcat gatacgaagg acgccctgaa actgtttcaa tagtcaaagt gtcatgatga
     3301 aaacgcatat taaacaccta atctttcttt tacatctgca taaatggcat ctgtaccaat
     3361 acctgttaat aagcccaagc cattcgttac tttagcttta attacatcag ggacaattgt
     3421 tgtacttact acagcatcat attcatcaat tttatttagc tcatcagcaa ttttagattg
     3481 ataaattttc acttgatctg ttaatccttc ttcttccaac cattttgata attttgctac
     3541 tgcaattgta gaagtagcat gacctgctcc acaaataact aataattttt tcatattgat
     3601 ctcttctttc ttattttaat tttatgatta agctttcttc actttgttca tgtagataga
     3661 agcacctact aaaacaactc ctaatccacc tattccaatt acaccaaagt aagatgctaa
     3721 taaaacaaac aaccctactg gccataatcc acataacaac atagttacta atcctgctgt
     3781 ccccacatcg aaacttgcta atgtaaacac atcagtgata attggtgcca accatgttgc
     3841 catatataat gttgtaacaa catacaacga acatcctact aatgatctca caatatttcc
     3901 tttaaaataa ggaatcatta aacagatggc gaaagcaaag aatgctaaat ctccaaaagg
     3961 taatacttta tttcctggaa gaattagtgc tagcaataat gagattggta caagcaatag
     4021 agaagttgac ataatagttg gatgacccac agttaacgct gcatccattc caatgtttac
     4081 ttggcgacca tttaactttt tattcgtaaa ttctttcgcc gcttctgcaa ttggcattaa
     4141 accttccata aacattgcta ccattttagg cataatcttc atcactgccg cagtcataat
     4201 tcctaagttt ccaattcctg ctacgtcata gcctgccata atacccacta caataccgat
     4261 aattaggcca ataacaactg tgtcacctaa aacaccgaaa cgttttgtta atgtttctgc
     4321 atctgcatct attttattaa atccaggaat acgatcaaaa acccaattta ctggtttggc
     4381 aaatagtact cctgataatc ccatcatatg tgtgatagag attcctggat agccgaaaaa
     4441 ttcgctaaca gttttttgtg tacgatctgc taagaaccat tgtaaaatta ctgcgacaat
     4501 cgcgccaatt aagccaaacc aaaagtttcc cgtaacagat tgagtaatca tacctaggaa
     4561 aattgcgaac caaaagttcc aaatatcaac attcaatgtt tttgtccaac ccaaaaccac
     4621 aaatatgaag ttaagaatta atgaaattgg aatcattaaa attcccaatg atgtagagta
     4681 agccaaaggt ccaccaacac ctacgccaat atctaggata ttcaggccac caccgtattg
     4741 ttcccccatt tgttgaattg ctgttcctaa accatctgat aataaactta aaactaatcc
     4801 taaaccaata gaaccgatac caacagtaac gccggaaaca aatgctttac caggttttaa
     4861 tccaaataat atcccgatta caaaaataat cagtggtaaa aagacgtttg accctaatcc
     4921 aataaaccat tcaaaggcat ttaatacaat atccatctct ctattcctcc taataaactc
     4981 ctgcagaatg tactaactca ataaatgctt ctttcgtggt acatgcaagt aattcattta
     5041 ctttttcttc attttgaaat aggctaacta agttttgtag tgtatcaatt tgttcatgag
     5101 gttgtgacat tgccaacata aagactaaac gtacaggtat ttcatgagat ttatctgtca
     5161 tatctgaaaa taccagtggt tcctttaatg aggcaaaaca gatttgagag ctatttacat
     5221 gtgcggaatc tgtatgtggg atagcaaacc caatttgatt tacttctaat ccagttggaa
     5281 attcttgttc acggttggtg ataccttcta agaaactatc tttcacgcaa ccagcgtttt
     5341 ttaattgttc agacattact tgaaacaatt ctttttgatc tttcacttct aattgaaata
     5401 aacataattc tttatctaga tacattttta ttctcctttt tgttgaaaaa ttcttagtaa
     5461 tttttcgcta tcatgaactt gtaacaagcg atcgaccatt tctttttgcc caataaattg
     5521 gtaaagttct tcatatactc ctctaatctc agatacatct tcttcagcta aactaaccat
     5581 aatgactaat ttgacttttc gatcgcccca attaatcgca ttttttaatg ctacaataaa
     5641 aatgttggat tcaatgacca ttgatggatt cgcatgcggt aaagcaacac cactttctaa
     5701 attagtattc cccattttct ctctagagaa gatagaagca cgaaactctt cagtaactaa
     5761 tgaattggct tctagttcgg aaatcatcaa atccaaacat tcttctttgc tttccacctg
     5821 tactcctgtt tttactaaag atgattgaac aaatttagat atggtgggaa ctttaaccgc
     5881 tttaggaatc agttcatttt gaataaatcg cgaaggttct tctattaaat atttagtata
     5941 agcttccata atattcatat aatctgtttt acttaccaat ggagataccc ttactaaggg
     6001 tataccacct gcatcaaagg aaagagagga aataatcaaa tcaacttccc caatttcagc
     6061 taaatgaaac ttatctaaag ttgatatttc tacattatct tttgctggta aaaaccgtcg
     6121 aactttattg taaattaatt gcgcagaaga aataccgtat tggcaaataa tgatgatatt
     6181 atttgctttg gactgatctt caatagcaat ctgaaaatga atcaaaatta aggaaacttc
     6241 atcgtccgtt aaagtcacat caaattcttc ttcaaggatg gacatggcgt accatactat
     6301 gctaaagagt tctgaatatt gagatttgat attttctaat aacggatttt gaattttaat
     6361 accttttttt aaacgaagga ccattgctgg cacatgatac aataaggatt gatataatcg
     6421 atcattttga gtcaaatcaa ttttttcaat ctcactcatt ctttcaatca atttatgaac
     6481 gctcatagcg tactcttcat tgctggtttt taaattattt gtcacacgat gtgcaaatag
     6541 ttgacgacat agatattctt tatctgcgtc ataaaatgga agattcaatt ttgccttcaa
     6601 ttcttcaatt aaaccatttg ccacaatgta cgtttccata tagcgaatgc tattgaagag
     6661 gaattcttct tcaacagtta aatgatgacc tttcagtaaa cggttacatt ggataactaa
     6721 agtagaaatc aatgaccgaa catagtaatc agaaaccttt tcagtcagtt cactgtaatc
     6781 attgaatagc aaatcatgaa tgacactaat aacttttgaa tcaaacaaca tatttaaaac
     6841 ttgaaaaaat gtttttgatt ccatgtcatt cgcataatag aatacaagtt gcttgattgc
     6901 tcgttgaatt gcttgttcat taccagtaaa actaattcct tctgcagatg actgcaactt
     6961 aacttctcca ctgccaataa ttttattgat aattctcagg tcattgtata acgatgtttt
     7021 actaaccata aaactttgag acaaatgagt taaagaaaca ggcttatcac caaacaaaac
     7081 ttctttaatg atctcgacac gtcttttttc tggagaataa atatcttttt caagataatt
     7141 ttctaccgat gaaatcaaat gttcaacgtt tttcctatca cctatgagtt tgatacccaa
     7201 agaaggtaat cgctcaattg ttacttggta attaactaca taatcttcca acaattcaat
     7261 atcttgataa atggtcttgg tcgacactgc caatttatca ctaaaaaaag aagcgggttt
     7321 aaagtcttct tgtcgactta ataattttaa taaatttaca tgtctttcaa tcaaacttgt
     7381 tcacctctta acgctagcat ttgttacctc ttttcattat ttattctagc actggaaccc
     7441 ccttccctca attacatcta tgtcaataat acttgtggaa gtttgttgta aaggaaatcc
     7501 aaataatctt aaaaaatgag aaacaaaaaa ggagaaatcc ttaaaagagc taagatattc
     7561 caatagaaaa tactccttat tatggaagta atatgaaata ttacaattga aaaataaaaa
     7621 caatataatg gcgtcactaa aattcgtatt tttttattat aatataactt aattacaaat
     7681 gctcatctat gaacaattga gtccta
//
LOCUS       DACEZN010000128         7599 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEZN010000128 DACEZN010000000
VERSION     DACEZN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.128"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(138..428)
                     /locus_tag="IXH60_002200"
     CDS             complement(138..428)
                     /locus_tag="IXH60_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991099.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IXH60_002201"
     CDS             complement(546..806)
                     /locus_tag="IXH60_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991100.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IXH60_002202"
     CDS             complement(943..1356)
                     /locus_tag="IXH60_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1991101.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IXH60_002203"
     CDS             complement(1812..2297)
                     /locus_tag="IXH60_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991102.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2681)
                     /locus_tag="IXH60_002204"
     CDS             complement(2436..2681)
                     /locus_tag="IXH60_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1991103.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="IXH60_002205"
     CDS             2874..3674
                     /locus_tag="IXH60_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1991104.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="IXH60_002206"
     CDS             3693..5561
                     /locus_tag="IXH60_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1991105.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="IXH60_002207"
     CDS             5554..6045
                     /locus_tag="IXH60_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1991106.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="IXH60_002208"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="IXH60_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1991107.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="IXH60_002209"
     CDS             6605..>7599
                     /locus_tag="IXH60_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1991108.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1443 g         2236 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aagctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatgcgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gttcccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DACEZN010000129         7521 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEZN010000129 DACEZN010000000
VERSION     DACEZN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.129"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(235..960)
                     /locus_tag="IXH60_002210"
     CDS             complement(235..960)
                     /locus_tag="IXH60_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991109.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1114..1440)
                     /locus_tag="IXH60_002211"
     CDS             complement(1114..1440)
                     /locus_tag="IXH60_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991110.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1477..1842)
                     /locus_tag="IXH60_002212"
     CDS             complement(1477..1842)
                     /locus_tag="IXH60_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1991111.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1879..2094)
                     /locus_tag="IXH60_002213"
     CDS             complement(1879..2094)
                     /locus_tag="IXH60_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1991112.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2223..2558
                     /locus_tag="IXH60_002214"
     CDS             2223..2558
                     /locus_tag="IXH60_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1991113.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2634..4631)
                     /locus_tag="IXH60_002215"
     CDS             complement(2634..4631)
                     /locus_tag="IXH60_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991114.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4729..5004)
                     /locus_tag="IXH60_002216"
     CDS             complement(4729..5004)
                     /locus_tag="IXH60_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1991115.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5236..5970
                     /locus_tag="IXH60_002217"
     CDS             5236..5970
                     /locus_tag="IXH60_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1991116.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            6009..7151
                     /locus_tag="IXH60_002218"
     CDS             6009..7151
                     /locus_tag="IXH60_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1991117.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2542 a         1377 c         1078 g         2524 t
ORIGIN      
        1 acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat
       61 atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg
      121 ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag
      181 tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc
      241 aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga
      301 ataatctttt ttcccatttt caagtaaccg aagtggaata tctttaccaa ctttctctgt
      361 cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt
      421 agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa gtttccaagt
      481 aggtggtata gctgtgtatg tttctacaaa tgaattaagt ttttcattaa gtgacttctc
      541 ttttatattt ccatctaaaa actctgtata tatggcttga gctatactaa aacaaagctg
      601 gccttgttct aataatttta catcattagt tagtaaagct gtgttcacgt tatcaatcgc
      661 ttcaagaaga gtatctttgg tgtccctaac agctttaggt gtgccttctg gaatttccct
      721 acttgatacc tcgtccattt tatcttccat aaacccctta aaatcatatc tttctcccaa
      781 atccatagaa gtttttgaga aagctttcat ttctgagtct atttcttcaa taatatttaa
      841 catgtctgat acatattgtt taggctgatt cgtttttaat ctatatgtgg taaatatctc
      901 aattcctcca attatcaata aaattgccaa acaaatccat aataattttt tctttttcat
      961 ttttcctcct atacactgct gttataacag tgactaatgt aaagattttc ccagcaatca
     1021 tcagactatt atttacaggg aatcaaaaaa actccttagt aagcttacac ctttaggagt
     1081 tcctgtttga acggtatgca taatacattt aaatcaattt aacattaaca cctcagtata
     1141 tggtatctct acatatacac ggtctttttt tggatagtac tcgacattta tattgatagc
     1201 acttccattg tctaacgtag ctttagcccg gtacgtgtta tcaccattat catgagtgat
     1261 cgtcacatca tcacatgtcc gatttaagtt atactgttct tgtaaaatct gagttaccaa
     1321 tggtacagat gacttctcta gtccagatga acccgtcatt cttgaaaaaa ttatacctat
     1381 aatagccaat acaacgatac ctataatgac aaacttctta ttcatattta ctttattcaa
     1441 ctttttatct cctatctcca aaatgatttt ctctcatcag taactcattg acatccccaa
     1501 tagtacacca aagatggctc cagctacccc tgctatcatc aaaatcgtac ccatttgttc
     1561 atctttctct cgatataagt aacccataat aacagcaaca gcaccaaata ccacaggaat
     1621 aaatgctaat gatactacca tagaaatcca accaataatc aaatacagct tccctgtctc
     1681 ttgtccatgt acgatttgtt ccttcatttc attaattgtc tgctcagtac ttttctttac
     1741 tgtgtctgtt ccggttgtct catctgtttc ttgtaatgct ccgcaaaact ggcaatactt
     1801 actttcattt gttatttctt taccacattt tctacaatac atgtcttctc ctcctaaaat
     1861 cattcaattg cttattagct aactgtttac tttattaaat acatcagaaa acagctcatt
     1921 tattggtaca cttaatatct cagaaagcat tttcatttga tagggtttag gaatcgttct
     1981 ccctgtttgc cacgaactta cacattgctg tgtaacattc atttttttgg ctaactcagt
     2041 ttgggaaagt cctttttctt ttctgtatcg gctgagatta cttacttttt tcatgattct
     2101 cccctcctgt tttattatat acaacaatat gatgtatttc aactcataaa atatataaat
     2161 acttcaaatg tcattacaaa tacaacaact tgttgtactc tactgagaag gggggtgtat
     2221 taatgaaaca gaagcaatat gcaaaggtca ttagagaaaa gcgaaaagca ctcgggtgga
     2281 cacaaaaaga attagctaaa aaaatcttct ctacacaaca ggcagtagca agatgggaaa
     2341 attctgtaac agaacctaac ctcgatagct taacagctct ttctagagcc ctaggaaccc
     2401 ctgtaagcca ttttctagac aatgttgaca tagattatga ggaagagttt ttggctctct
     2461 atcgctctct tagtactgaa gatgcaattc gaacaattga ctatatgaag ctattaaaaa
     2521 ggcaagaaaa tgaacgcaat cagctattga aagattgaaa aataaataac ccaccgaact
     2581 ttaacggtgg gtgtattgag atgaaataag attaagagct atggataacc tgtctagttt
     2641 aatataattg ggtctcttct attccttact ctccgtccat catctcttgt tacacttgta
     2701 aattgataaa taggaactaa ttcaactcct gataatatca attcttttat tacttcatct
     2761 atccctgccc ctttcgctag atatactaaa aaactactgc ttgctttcac attgtatagt
     2821 ttcttaatat ccttagaagc tcgttcaaga gtgaatcctt gtttaacaaa gtagcctctg
     2881 ttttctatta aagcattttt cacgctgtta tccctgacat ttcgtaggcg ttcacaaaca
     2941 gtgttaataa gttctttttc tgtcattaga tatgtccctt gccacgataa gccctgtaaa
     3001 acttcatgtg cttcatctaa aatcaattgt ttttccttgc taagttctga tcgggaattt
     3061 tgaggaaata ctcttttagc ctctttttcg ttaaaacata tcttaaccca ttcataagaa
     3121 aaatattgat atgtaaaatg aaatttttcc agaagtggaa ggatatcttc attcaatttt
     3181 tgaatcacag aagttgcatt aaaaacgtct aatcgataga cattgcttct cttatgttta
     3241 aagtttccta acgctttctc ttcctttaac ctatttaggt attcgagcat cttcaaagga
     3301 acatcttcat catctaattt ttttatcaat cctataaaag tcataagtga tagtaactta
     3361 ttacatttat ctatgtcata agttttacca tagtaagaac tcattttttt agctaattgt
     3421 tcccccgaca aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa
     3481 agcaagacat catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt
     3541 ttgtctttca ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga
     3601 aaatgtgtag gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg
     3661 tctttcttta aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg
     3721 gaatgacaat aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta
     3781 tgatcgttgt gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca
     3841 atggttaaca atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag
     3901 tctttaaacc actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg
     3961 gtttcattga ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc
     4021 atggtaaaat caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc
     4081 actgcatctg acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca
     4141 cacccgggga tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta
     4201 attgcttctt ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata
     4261 cttttttgta tcgcaaacgc agaattatga actaaattga tagataaaac cttttctatg
     4321 cttaagtatc tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca
     4381 gtgttatcga catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct
     4441 ttattctttg tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca
     4501 tctcttgtaa cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt
     4561 tttgtaactg gcttctctaa taatttttct ttatctacat aaattggaat attaatcatt
     4621 gatgtttcca tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata
     4681 ataaaaacaa actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata
     4741 ccgttccatg aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat
     4801 aacaggtaac ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata
     4861 ttcacttgcc tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag
     4921 cataggcttt agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat
     4981 gctatcaatg atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt
     5041 tctaagaatt atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta
     5101 atcaaacaaa aaatgattac cagttcatcg acatacacat aatacctcga taaaaaaggg
     5161 gtgtcaatag ttttgtaaaa tttatttaca acaccccttt tttgaggtat actaatttta
     5221 ggaggtgtgg tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata
     5281 ctcggaacaa aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt
     5341 cttatgatta acaacggtca attaattgac aaaaagacta tctacgaatg ggagaaggga
     5401 acctatttac caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata
     5461 gaggagttag tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat
     5521 agcattgtac tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa
     5581 cagttcccac cagtacactc taatttagat acatggctgg atagatattc caaattagaa
     5641 ccagagatgc aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt
     5701 agtcttttca atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt
     5761 gacaataaca ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat
     5821 gaatggctac ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt
     5881 actgataata taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag
     5941 aaaaaaatga agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag
     6001 atgatttcat ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt
     6061 ataaaggaca cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag
     6121 gttttagtag caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat
     6181 acggtgttaa aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa
     6241 ctcatcagcg taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata
     6301 atcaaatact tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc
     6361 aaaaagtggt agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga
     6421 ctgcacaaat tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa
     6481 aaacacttct tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg
     6541 agcttaataa tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct
     6601 tcagattatt ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag
     6661 acatagacac gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg
     6721 gtaaagttat aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata
     6781 ctgagactct ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac
     6841 ttggatacaa tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat
     6901 acattccaaa cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc
     6961 gtattacacc tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat
     7021 ctgtcaaggt agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct
     7081 atgcacatat tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg
     7141 ctcatcagta aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa
     7201 agctagaacc cttatatatc aagggttcta gctcctataa caggtttacc caacactgcc
     7261 ttccaatgac tcttttggga atcagaggaa agaatcaggc tttatagaga tatttcatct
     7321 aacaaattac tttagactga cagtatatat cagcaggtac tgtcatcggg aacgtagcca
     7381 atatcgtatc caaaacagca ggtagagaag ctatttgagg attcaagtta ttctatttca
     7441 accatgaagt taagttcatt aaaaataaag atagttggta tttcaataat ttttgtatag
     7501 agttaatcac tttattgatg c
//
LOCUS       DACEZN010000130         7429 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEZN010000130 DACEZN010000000
VERSION     DACEZN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.130"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..152
                     /locus_tag="IXH60_002219"
     CDS             <1..152
                     /locus_tag="IXH60_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAQ1991118.1"
                     /translation="YIINTENITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKI
                     ENESF"
     gene            328..1071
                     /locus_tag="IXH60_002220"
     CDS             328..1071
                     /locus_tag="IXH60_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1991119.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1064..2644
                     /locus_tag="IXH60_002221"
     CDS             1064..2644
                     /locus_tag="IXH60_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1991120.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2895..3374)
                     /locus_tag="IXH60_002222"
     CDS             complement(2895..3374)
                     /locus_tag="IXH60_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1991121.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3390..4142)
                     /locus_tag="IXH60_002223"
     CDS             complement(3390..4142)
                     /locus_tag="IXH60_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1991122.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4259..4329
                     /locus_tag="IXH60_002224"
     tRNA            4259..4329
                     /locus_tag="IXH60_002224"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4291..4293,aa:Gly,seq:ccc)
     gene            4695..5420
                     /locus_tag="IXH60_002225"
     CDS             4695..5420
                     /locus_tag="IXH60_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1991123.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5674..5940
                     /locus_tag="IXH60_002226"
     CDS             5674..5940
                     /locus_tag="IXH60_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1991124.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            5937..6368
                     /gene="ruvX"
                     /locus_tag="IXH60_002227"
     CDS             5937..6368
                     /gene="ruvX"
                     /locus_tag="IXH60_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1991125.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6394..6696
                     /locus_tag="IXH60_002228"
     CDS             6394..6696
                     /locus_tag="IXH60_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1991126.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            6886..>7429
                     /locus_tag="IXH60_002229"
     CDS             6886..>7429
                     /locus_tag="IXH60_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1991127.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSL"
BASE COUNT         2390 a         1267 c         1511 g         2261 t
ORIGIN      
        1 cttatatcat taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat
       61 ttgcaaatgg agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta
      121 tgcaagaaaa gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt
      181 tagaagagtg ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc
      241 ttttttgtta tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa
      301 gtaataacaa acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac
      361 gggaaaaaac aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga
      421 tttttaggac ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt
      481 gaaccatcat cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt
      541 atcaaacaaa aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct
      601 ggagaatatt gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag
      661 cgtttagcag aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca
      721 agtttttcac atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca
      781 gatatttggg tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg
      841 aaagaaatga tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta
      901 ttggataccg cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt
      961 tatcaggggg cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt
     1021 tatttgaaaa tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt
     1081 attagaatta attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag
     1141 agagaaagga aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc
     1201 agggctgatt tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt
     1261 gccaggtttc tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat
     1321 atcagtcatt tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc
     1381 ttttggacaa gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc
     1441 ttttgttttt ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat
     1501 tttgcttact atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg
     1561 tctggctagt ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa
     1621 aacagtttct agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg
     1681 gatgaatcat caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat
     1741 attattgcca ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg
     1801 gggaatacta gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc
     1861 aaagcttaca gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa
     1921 aaagaaccaa aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc
     1981 taatttgatt atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc
     2041 ttttgcccta ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt
     2101 tttcctggta gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa
     2161 tcttatttct ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa
     2221 ccaatattta aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg
     2281 gatcgcatta ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct
     2341 atttggttct ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg
     2401 gttgctgctg ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt
     2461 gggtttagtc gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata
     2521 cggaatgttg gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt
     2581 tctaatactt tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga
     2641 ataaagaaga ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt
     2701 gttcaaaagt cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt
     2761 ttacaaaact ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc
     2821 aatgtttgtg agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct
     2881 ttgttacact tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc
     2941 tttactttga aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg
     3001 attgctccag caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca
     3061 gtaacgagat ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg
     3121 ggaatattga aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat
     3181 gctactccta aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag
     3241 atccagtaga tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta
     3301 ggtacatttg taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata
     3361 aatatcgtcc ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc
     3421 gcaaaaccga tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca
     3481 taatgtccag ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc
     3541 atcacacatc cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag
     3601 atagcggcta tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa
     3661 aatttcaaac tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt
     3721 aaatcggtca tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta
     3781 acgatttgca accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag
     3841 taaagttctt ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt
     3901 actacttttt ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta
     3961 cgatcaagac caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca
     4021 gaagctaaag caattcgact catcgtatct tctacccgat acatctccgc attgctttcc
     4081 atcatgattt tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc
     4141 atagcatctt ctccaataat tgattgatta gttttatttt actacaaaac atatcggaag
     4201 cgtaaagaat aaaaaaactt gaggaaggag tgaaagaggc gtatactgtt tttgtatcgc
     4261 gggtgtagtt tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg
     4321 tcacccgctt aaaagaggct gtgaaaaagc tgccctgcat caagtaataa gaatagtcaa
     4381 gcaaaaaagc ttcttatgtt tttgacttgg ttggacttat taccgcagat gcaagctttt
     4441 gaacactgtt taccaagagg tcgtacagaa gccgtttagt tttgagtatt aagaaaaatt
     4501 ttttgaatga taataaatac cttgtaaata gtgttttgtg attgagtaga gacgatgaat
     4561 ccgtctcttt ttttgtattc ttaagccgtc taaaaactgg ttttattttt ttactgtttc
     4621 tatttctttc cagtatttat ttatagggga acatatgtta agatttaggg aactgttagt
     4681 aaaggagtaa agaaatgagc aaaaaaacaa ccaaggcgat cgtggggatc gtcgctgctg
     4741 tgattgcagg ggcactcggt atttccacca cacaaaaaga ttcagatatc ttccaacaaa
     4801 ttacaaccat ttttgattcc acaccgcaag cttctcaagt agcggatgtg ccagaatatg
     4861 atggaaaaca tcaagaaata gaaatcaatg ggaatcagcc ggagtttaca aaagaggatc
     4921 ttagtctttc caaagggaca tgggaaagct attcaaatat agacaaattg aacagagtag
     4981 gacaagccaa tgcgatgttg ggaaaagact tgtttcctac tgaaaagaga gaacctttgt
     5041 atattgatcc gacaggttgg aaacaaaaga aattaagtga cggacaatgg ttatacaacc
     5101 gcagccattt gattggtttc cagttgactg gtcaaaataa taacattaaa aacttaatga
     5161 cagggacacg ttcgttgaat gctccgtaca tgcttgccca cgaaaatgat attgcggcat
     5221 atatcaagga aacaaatcat catgtacgct atcgtgtcac tcctcatttc gagggaaatg
     5281 aactggtagc aagaggtgtg cagttggaag cagaaagcat tgaagacaaa aaaattgaat
     5341 tcaatgtctt tatctacaat gttcaagatg gctacaccat caattatgag acagggcaag
     5401 caacgaaaac gaaaaattag tagcagcaat tcgaattttt tatgaacttt atgaaaaaat
     5461 tgtgtaaaaa catactttca aagaaatata gcgtatactt tatggaaaga gggcttttga
     5521 aaaagccaca gaaaaaagga aaaatcagac aaatcgtgtt tcttaattta ttcagaagtt
     5581 tggaatattt gtttgtagtt ttccctttct tcgagtaaaa taagggtgta gaaggtaagc
     5641 tggaattacc ttgcgggaaa gaggtgtaga atcatgggtt ttacagatga aaccgtacgt
     5701 tttggttttg acgatagtcg caaaaaagaa gtgagcgaaa cattagcgat tgtttatcgg
     5761 gctttggaag aaaaaggcta caatccaatc aatcagattg tcggctactt gctttctgga
     5821 gacccggcct acattcctcg ttatcgtgat gcacgaaatt tgattcgccg tcacgaacgc
     5881 gatgaaatca tggaagtaat tgtcaaagac tacctctcaa atcatggagt gaatttatga
     5941 gagtaatggg cttggatgtc ggttctaaga cagtaggcgt tgctgtgagc gatccctttg
     6001 gctggacagc tcaaggagta gaaatcattc gaatcaatga ggcagaagaa gagtttggtt
     6061 ttgatcggtt gaaggaatta gtggcgcagt acgaagtaga ccgttttgta gttggattac
     6121 ctaagaatat gaacaattcc atcggaccaa gggcagaagc ttccatgtca tatggagaga
     6181 agatcaaaga actattctcc ttgccggttg attatcaaga tgaacgacta accactgttc
     6241 aggcagaacg tatgctagta gagcaggcaa atacttcaag agcgaaacga aaaaaagtca
     6301 tcgataaatt agcagctgtg atgattttac aaaattattt agacggctca ggtaagcaat
     6361 tattttaata ataaaaagaa aagaggtaat cttatgacag aacataacca ttcccacgaa
     6421 cacgaaggac acgaacacat cacattggtg gatgaccaag gaaatgaaac attatacgaa
     6481 atcttgctaa cagttgacgg acaagaagaa tttggacgta actatgttct tttatatcca
     6541 gctggtgttt cagaagatga agatgtagaa ttacaagctt atgcatacat cgaaaacgaa
     6601 gatggaacag aaggcgagtt agaacaaatc gaaacagacg cagaatggga tatgatcgaa
     6661 gaagtattca atacattcat ggcggaagaa gaatagaaca aaagaatcga taaggtgtaa
     6721 gaaaagctgg actctctgaa aagtagagcc cggttttttc gtttagcact aaatttcctt
     6781 aaaaagtaga aaagctttcg aagcctcgtt tgtttctttt actgtataat aaaagaaaag
     6841 agtaacaatc gctaaaagat ataaacaaga gaaaggaaat acaagatgat tggtctgatt
     6901 tattttgtgg tgatcgttct ggcgaacaca gttggcgcta tttccgggat gggtggaggc
     6961 gtgttgatca aaccgattct tgatcttatc ggcgcccatt ctgttgcagg gatttctttt
     7021 tactcaacag ttgcggtatt cacgatgtcc attgtttcga ctgtgcgaca agtatcaagc
     7081 gggaaatcac tgaattggca gattgtcggt tgggtatcta gtggagctgt tgttggagga
     7141 gtagtcggca acattgtatt cgaagttttt ttacagttat ttgaaaacga gaagcatgtg
     7201 cagctgatcc aaattttttt gacggtattg accttggttt ttgcgttctt ctacaccaag
     7261 catcatcagc caaaatttca tttgacgtca tggacttggt accttatatg tgggggggtt
     7321 ctgggctttt tagctagttt tttaggtatt ggtggtggac cagtcaatgt ttctttgttg
     7381 atgctgatgt ttgcattgcc aataaaagaa gcaacactat attctctat
//
LOCUS       DACEZN010000131         7400 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEZN010000131 DACEZN010000000
VERSION     DACEZN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.131"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            68..151
                     /locus_tag="IXH60_002230"
     CDS             68..151
                     /locus_tag="IXH60_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1991128.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="IXH60_002231"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="IXH60_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1991129.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="IXH60_002232"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="IXH60_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1606..2271
                     /locus_tag="IXH60_002233"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="IXH60_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="IXH60_002234"
     CDS             2307..2552
                     /locus_tag="IXH60_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1991130.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..3524
                     /locus_tag="IXH60_002235"
     CDS             2655..3524
                     /locus_tag="IXH60_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1991131.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3505..4239
                     /locus_tag="IXH60_002236"
     CDS             3505..4239
                     /locus_tag="IXH60_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1991132.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4272..5184
                     /gene="ant(6)"
                     /locus_tag="IXH60_002237"
                     /pseudo
     CDS             4272..5184
                     /gene="ant(6)"
                     /locus_tag="IXH60_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5181..5723
                     /gene="sat4"
                     /locus_tag="IXH60_002238"
     CDS             5181..5723
                     /gene="sat4"
                     /locus_tag="IXH60_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1991133.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5816..6610
                     /locus_tag="IXH60_002239"
                     /note="aph(3')-IIIa"
     CDS             5816..6610
                     /locus_tag="IXH60_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1991134.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6718..6906)
                     /locus_tag="IXH60_002240"
                     /pseudo
     CDS             complement(6718..6906)
                     /locus_tag="IXH60_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6886..>7400
                     /locus_tag="IXH60_002241"
     CDS             6886..>7400
                     /locus_tag="IXH60_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1991135.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2593 a         1119 c         1555 g         2133 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3481 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3541 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3601 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3661 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3721 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3781 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3841 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3901 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3961 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4021 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4081 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4141 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4201 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4261 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4321 aggatgaacg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata
     4381 cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt
     4441 atatctaatg atgactggct taatcaattt gggaatataa taatgatgca aaagccggag
     4501 gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat
     4561 tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc
     4621 gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg
     4681 actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat
     4741 gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt
     4801 gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag
     4861 gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg
     4921 tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa
     4981 aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg
     5041 gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg
     5101 gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat
     5161 atagaagaga ggcgggaaca gtgattacag aaatgaaagc agggcacctg aaagatatcg
     5221 ataaacccag cgaaccattt gaggtgatag gtaagattat accgaggtat gaaaacgaga
     5281 attggacctt tacagaatta ctctatgaag cgccatattt aaaaagctac caagacgaag
     5341 aggatgaaga ggatgaggag gcagattgcc ttgaatatat tgacaatact gataagataa
     5401 tatatcttta ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga
     5461 accggtacgc ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag
     5521 gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa
     5581 tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca
     5641 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     5701 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     5761 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     5821 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     5881 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     5941 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     6001 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     6061 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     6121 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     6181 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     6241 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     6301 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     6361 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     6421 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     6481 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     6541 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     6601 attgttttag tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat
     6661 ctaatctttt ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta
     6721 aaagttcgct agatagagtt ctatattaga gatgtaaaga gttttggtga agaggtttgt
     6781 gagtatggct tttataacca gggtgcaatg agaagcacta acagcagcta gcttaagaac
     6841 gcataggata tactttttat ggaagtcaca gaggaggaat aaaatttgaa caggattaac
     6901 agagtaacct ctattcttat tcagctgcaa tcaaaaaaaa taattcctgc caaagaaatt
     6961 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     7021 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     7081 cttccgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     7141 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     7201 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     7261 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     7321 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     7381 ccggaaagca gaatgataga
//
LOCUS       DACEZN010000132         7397 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEZN010000132 DACEZN010000000
VERSION     DACEZN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.132"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..251
                     /locus_tag="IXH60_002242"
     CDS             <1..251
                     /locus_tag="IXH60_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1991136.1"
                     /translation="PKGLSKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLN
                     FLVEQDILNERIKYLEIGRPLTCYELTKNGRKKLTDYC"
     gene            complement(353..1372)
                     /locus_tag="IXH60_002243"
     CDS             complement(353..1372)
                     /locus_tag="IXH60_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1991137.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(1391..2449)
                     /locus_tag="IXH60_002244"
     CDS             complement(1391..2449)
                     /locus_tag="IXH60_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1991138.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(2671..3819)
                     /gene="nagA"
                     /locus_tag="IXH60_002245"
     CDS             complement(2671..3819)
                     /gene="nagA"
                     /locus_tag="IXH60_002245"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1991139.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(3895..4731)
                     /locus_tag="IXH60_002246"
     CDS             complement(3895..4731)
                     /locus_tag="IXH60_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1991140.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(4915..6132)
                     /locus_tag="IXH60_002247"
     CDS             complement(4915..6132)
                     /locus_tag="IXH60_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1991141.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            6515..6598
                     /locus_tag="IXH60_002248"
     tRNA            6515..6598
                     /locus_tag="IXH60_002248"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6549..6551,aa:Leu,seq:gag)
     gene            complement(6838..>7397)
                     /locus_tag="IXH60_002249"
     CDS             complement(6838..>7397)
                     /locus_tag="IXH60_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991142.1"
                     /translation="LFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTY
                     VNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNK
                     KTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSV
                     QKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         2294 a         1532 c         1208 g         2363 t
ORIGIN      
        1 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
       61 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
      121 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
      181 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
      241 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
      301 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
      361 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
      421 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
      481 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
      541 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
      601 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
      661 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
      721 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
      781 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
      841 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
      901 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
      961 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
     1021 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
     1081 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
     1141 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     1201 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     1261 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     1321 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     1381 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     1441 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     1501 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     1561 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     1621 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     1681 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     1741 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     1801 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     1861 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     1921 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     1981 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     2041 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     2101 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     2161 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     2221 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     2281 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     2341 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     2401 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     2461 taattatgtt catttattca caataaaaaa aagcttttat tttgctaata cgtattaaca
     2521 tttttaattt tatctttaca ttgttactta ttgaacaata ggaaattaaa gaaacttttg
     2581 aaacttatgt aaagtgattt ttactagaac acaaaaaaaa ccttaaaacc aaacttaaga
     2641 agtttagttt taaggttaac ttaaattata ttatgcgtgg aaacgttcca cgccatctag
     2701 ataagttgct tccaatgtca tatcagggtt caatacgata aagtctgcat cgcgtccttg
     2761 agcgattttt ccacatgtat catcgatttt acagctgatt gctggaatca gactagccat
     2821 catgactgct tgttctggtg ttgctaatcc ccagtctaca acatttttaa ttgcttcttt
     2881 caatttcaat atacttccag ctaagttgcc agattctaga cgtgctgttc cttcttttac
     2941 tactactgga aattcgccta aattgtaatt tccatcaggc atcccaccgg ccatcataca
     3001 gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc attaggattc ccgcagcatt
     3061 tggatgtaca tgatggccat cacaaatcaa ttcagaaaag acatgttcta aagataataa
     3121 cgcacccacc atacctggtt cgcgatgatt caatccacgc ataccattga aagcatggac
     3181 aaatacactt gcgcctgctt caacagcttt ttgcgcttct tctagtgtag cattactgtg
     3241 tcctagagaa acaactactc cttcttcagt cacttgtttg acaaactctt ctacaccttg
     3301 acgttccggt gctaaagcaa tttttttgat taaaccacct gaagcttctt gccattcatg
     3361 gaaagtatcc aaatcaggat ccccgaaata acttgggttt tgtgcccctt tgtgttcttc
     3421 tgtgaagaaa ggaccttcaa aatagattcc tttgatttta gctcctggtg cttcctgata
     3481 cattttgccg attgtctcag ctacatcttt taaacgttct ttgcttgacg tcaatgttgt
     3541 tggtaaaaat gaagtcactc cacaagataa cagaccttca gacatcactt tgatgccttc
     3601 tgcatcatta tccattacat catgattcat atagccgtga atatgtgtat ctaccaaacc
     3661 aggagcaatc catttgccgc tttggtcaat gatgttgact tctccctcag gtagttcttt
     3721 ggtgtattca ccaaaaagtc catcggtaat ttcaagatag cctgcatgtt tgacacctga
     3781 cgctaaaaag aatttgtcag catagataaa agttctcatt ctattctctc cctttgtttt
     3841 tctacctcta aagtactctt atttagagaa gaagtcaaga atggtctaca ccatttacaa
     3901 attttttgaa atttgtttca cagcttgtgc taagccttca tagaatgctg ctctttctgg
     3961 ttcttcttta cgcttgtata ttaagctttt aagcacaaat aaatcattga ggtaatagta
     4021 gctattcttc tcttgagccc aatagattcc ttgattcacc tgttctaacg cttcgtcgat
     4081 ttcattttga tggaataaga aatttccata attataaaaa atttgcgaaa attcactttt
     4141 tgatcgttca tcagctgatt gatgcagcag ttggatgctt cgactcaaat agtagcgtgc
     4201 ttcagccgtt tttcctgcca caccatatat ttttccgata caactaatca gttgtatttc
     4261 tgtgtcagaa acatggattc tttctttttg gctagagtag cttaatgctt ctttcaaata
     4321 ataaagggac tgagagatat tttgacgcaa cgtgaattcg caaacaccga gataataata
     4381 atagcgttga aagtcagttg cttgatacag attttcagaa atatcagaag attttagtac
     4441 ttgttctaac ttccgataat cacgtttttg gaaacaacta tccaacagtt caaataattt
     4501 atcgtttgtc cgagtagttt ttcgagagag attcatcaca tgatcaatcg ttacccctag
     4561 ccgttgacaa atctgcatca tcaccaatac attcggcagt tcctcattat tttcgatccg
     4621 acttaataca ctttgtgaac aaatatcatg cgaaagcatt ttttgcgtca ttttttgttc
     4681 ttttcgaatc tctttaatga tcgttccaaa ggactcgatt aacgtttcca taagtatcct
     4741 ccccaaaata tgcgtattcg cattctattc gtattttact ttaggaagtc gcaaacttca
     4801 agaaaagact tgcaactaaa taatatactt tagttattta agaaaactcc actagaaaga
     4861 gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa tttggttttt aaaatcattt
     4921 ttggcgatag gttgctagga attctctcat tttcttagct gtttgtttca aatcatccat
     4981 tttaggaaga taaacgattc gaaaatgatc tggctggttc caattaaatc caccaccatg
     5041 tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg aatttttcat catccaaaat
     5101 attgaatcgc tttgtatcaa tttttgggaa tatataaaaa gcagcttttg gttttacggc
     5161 tgaaagacca ggaatatcat tgattgcatt atagatatat tctctttgtt catagatcct
     5221 tccgcctggc aggagcagct catcgacact ttgataaccg cctagcgcgg tttggataat
     5281 ttgctgagac aatacattgg aacacaaacg catggaggac aacatattca gcccttctat
     5341 atagcctttg acgtgtgact tatctccact taggaccatc cagcctactc gaaatcctgc
     5401 tacacgatga gatttggata gaccgttcag cgtaacaacg aacaaatcag gcgctaatgt
     5461 agcaatcgga atatgttcta gaccatccat cactaagcga tcatagattt cgtcggaata
     5521 aataatcagg ttattttgtc gagctacttc aacgatttct tctaataatt cttttggata
     5581 aagtgcccca gtaggattat taggattaat gataacaata gcttttgtcc gactcgttat
     5641 ttttgatttg atatcatcaa tatcaggata ccattctgcc tcttcatcgc aaatataatg
     5701 aacaggtgtg cctccagcta atgcaataga agccgtccac aacggatagt ccggcattgg
     5761 aaccagtact tcatctccat tgtccagcaa cccctgcata cacatcgtga tcaattcgct
     5821 cactccgttg ccggtgtaga tatcattgat cgtcacattc ggaaaatgtt tcagttggca
     5881 atattgctca attgcttttc tagcagaaaa gatccccttg gaatctgagt agccttctga
     5941 gtcacgggca ttcatgatca aatcacgaat gacttcgttt ggtgcttcaa atccaaaagg
     6001 tgcgggattt cccgtgttca attttaaaat actaatgccc tcttcttgca tccgctccgc
     6061 ttcttctaga acgggaccgc gaacatcgta actgacacct tctaatttgt tggatttttc
     6121 aaattctctc atcacttagc caatccctca tttcaaaaag ttagaacttt ccaaattttt
     6181 tgttatttac tatttcaata tcatactcca tcctagaaaa ttttgccact tatttaggaa
     6241 aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac ttaattgtca tgatagtcat
     6301 tccatagatt cgctgcccaa tccattgcaa ttccacaaat gattcctagc ataaaaccaa
     6361 gcaaaaaacg tccgaatgca agtccaatca atacacctaa gatcattccc agtcctgaaa
     6421 attctccgtc atatttctcc atcttaataa gtcctcccat ttttgagctt gacaactatg
     6481 taaaacctcg ctatcattag tgtgttatta ttacgcagat gtgttggaat tggcagacaa
     6541 gcatgattga gggtcatgtg agcgttagct cgtgtgggtt cgagtcccat catctgcact
     6601 acaactcgtg aagttaattt tgcttcacga gtttttttgt ttgaacgttt ctttatgcca
     6661 ttatatacat atcactgtat cctttacaaa aagaacgtac aatcatgtaa acagcctgag
     6721 gtgagtatat gaaggtcgga aacaagctac tcgtagaaac ttgcaagtgt taaaacaatt
     6781 aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc gagggatttt ttgtatatta
     6841 tctgtttatc accgttatta taaaggtaca aataccaagc acggttaatc caaattgcat
     6901 aaattcattt ttttgtacac tcatgctttt cattagctct ttaactgcca ttagtactaa
     6961 gatggcgata acaacataaa gtatatagta cgtccgcatt ccggaagccc atactgtggg
     7021 agaaaatccc ataattaacc ttgaacaaaa tcccattatc atcaaaataa ctaaaaaaat
     7081 agaagttttc ttattgtcaa aacttaaata aattccaaca attaaacata gtaataatgc
     7141 caagactact aaagtagcta tccaagtacc tggataaaaa agtgacagtt tcgttcctaa
     7201 ctctgtaaag agattattca ggttactact actccaaatc attgcccttt tatttccatt
     7261 gacgtaagta aagctttgac ccattgtatt accaaaataa ataatcaggt ttaagaaaaa
     7321 cggtaatgca gtcaatatac gcacataata gttttgacat ttcctatatg ttagaaggaa
     7381 aattaagaaa aatagca
//
LOCUS       DACEZN010000133         7066 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEZN010000133 DACEZN010000000
VERSION     DACEZN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.133"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            143..583
                     /locus_tag="IXH60_002250"
     CDS             143..583
                     /locus_tag="IXH60_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1991143.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(609..1766)
                     /locus_tag="IXH60_002251"
     CDS             complement(609..1766)
                     /locus_tag="IXH60_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991144.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1769..2125)
                     /locus_tag="IXH60_002252"
     CDS             complement(1769..2125)
                     /locus_tag="IXH60_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991145.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(2423..3397)
                     /gene="sgrA"
                     /locus_tag="IXH60_002253"
     CDS             complement(2423..3397)
                     /gene="sgrA"
                     /locus_tag="IXH60_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1991146.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(3593..4432)
                     /locus_tag="IXH60_002254"
     CDS             complement(3593..4432)
                     /locus_tag="IXH60_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1991147.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4617..5504
                     /locus_tag="IXH60_002255"
     CDS             4617..5504
                     /locus_tag="IXH60_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1991148.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(6088..6783)
                     /locus_tag="IXH60_002256"
     CDS             complement(6088..6783)
                     /locus_tag="IXH60_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1991149.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6767..>7066)
                     /locus_tag="IXH60_002257"
     CDS             complement(6767..>7066)
                     /locus_tag="IXH60_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1991150.1"
                     /translation="TLTDYCYKIELNQVRKEITCDMKLCFDKQMYVITAVGDVVQTNL
                     NELGHITIKFDGSHEPELPGTMYVEDKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2231 a         1386 c         1043 g         2406 t
ORIGIN      
        1 tttttcttga tttaatagta tttcttatct agctagttcc attatacgtg acaacatttt
       61 cttattttac acctctaaat ggcacttttc gactatttct tcatgttatt atttcataaa
      121 tacttagcta ttggaggaat gtatgatgat taaattgtat acaggttctg gctgctcgtc
      181 ttcaagacgc gcaaaacgtt ggcttatcgt ccatgatata ccgtttatcg aacagaactt
      241 aattcgtcaa ccactaacca aaaatgaatt tatccactta ctaaaattga caaacaacgg
      301 cgtgaccgaa atgctctcta ctcattctat tagttataaa aatcttgttc acagaataga
      361 tgaactcagt ttgagtgaac tatatattga gattcgtaca tccccttctt tattgaaaaa
      421 accgattatc tacgaccaat ctgcaaaact tttgactgga tttagcgaag ataaaatcag
      481 gatgttcctt tctaaaacta aaagaaagca agagctcaac gaactcttgc tttctggtca
      541 tcttgtcgat tttaaagaaa ataatctttt tgcaatgtct taattttggc ataaacaatc
      601 tgcttttctt aattaacgaa atccaatttt tccaatatgg atctattttt ctcattcctg
      661 atttttatga tttcagcttt cagacgcgcc caatcatttt ttgtaggttg tccattccag
      721 taaaatatct gtgtattcgt ttgatcatat gcctttttgt cgataggata atcgctaatg
      781 acaatatcag gtaactgatc agcggatgca ctatatttta ttgggcgttc agtcatagat
      841 aatatccttt tctttgccca taattcctgt gttttaccat actttgattg aatatagaat
      901 atgacaggtt gttcgtgttt tataaaaata tccctgacta acatacagta ttgaaaaatc
      961 aaccgtctgt tcttttcata ataagtatta agctctggaa ttctgcgagc taccccacta
     1021 aaaaactgtt gcatgattgg atacatcact ggaaatatat gctgaaaatc tctgtcttct
     1081 gctgattttc caaaagcatc caccttcttt tttgtcccaa aaacccttga atacaaatgg
     1141 atcattgcca aattgacata tagataattt ttttctgaag ttgtaaaaca aatcttgaag
     1201 tttttttcaa aattttctac aatagacgat tcaatccagc cccaactttc ttgtacttta
     1261 tctggagtat ccaacaaatc ttcttccaaa taggtgttca ttacaccaaa cataaaataa
     1321 aggtaagctt gctctgttgg agtgatgtga ttcatcgacc agaagtttgc ttcaaaaaaa
     1381 ggctctagta tgtaatgcgt aaactcttct tttgttatca atggatgcac aaatttgttc
     1441 atctcttctg ggacatacgt aatcgagtgg ccttgtttca ttcgttgcaa cgaaattctt
     1501 agtaggctga tccatttctt tctatctata ctacgcgtat atggcgcatg tttcgcaaat
     1561 ttttctaata gttgacgttt attttttata cttgataata tatcagaatg attttcgtcg
     1621 aatgactgcc aatacatcaa gtgaaaaaag tatcggatac ggtattcttc tccttctatc
     1681 ggattacctt ttttcaagct gcaagataaa tgatagtttt tcaaaatagt attaagttct
     1741 cttaattttc tttgaatcgt acgcaaactt atataatttt ttgactaaga ttttctaagg
     1801 attgataagt acctttgtaa agtgaatcca gtaaaatata accaatcgtt ccctctaaaa
     1861 gtatagaata aactttactc agtaaaaact gagaagaaaa ctcgactttt atcatcttgt
     1921 tttctgaaag aaaagataat ttatattctg gatacctttc gcaaaacgat tctgcttgtt
     1981 gaatcaccga attcatctta gccagggtgt agtttagatc taaagcgata tccttcacca
     2041 catataattt ctcgtctctt tcataacaat agttcaaaaa ttgatatact tgcctttcat
     2101 tgatatcaat aaattcttca aacaaagtac atactccttt ttatctatta tttagactta
     2161 ttccgacagc tacatgaata acgtacaaat agattcgtta ctgatttttc agagatacta
     2221 ccatgcgcag tgtgcattgt caattattct tgctcttatt ttccatttgt gttcctgtta
     2281 tttcctgttt ctcaaatatg ttagtaaaca aaaatagatc tggcaaagct acaacttcgc
     2341 caggcaatac agcactctcg aatgaaatat tcactcaaaa atctatcgtt ttattcaatt
     2401 ttagatctgt tgcttttatt ctttatttca ctcgatggaa gagagaacga aaatagcttg
     2461 ctcctacaat caaaatgagt aacacaccca tccatgtaat tagcttgttc aaggttctac
     2521 taccagtttc tggtaaatcg ctttttctat tatctgtttc tttcgctttt tcttgatgat
     2581 caattccacg agaaacaaaa tcaggctgtt ttagcacact ttttaggttg ctccctgcat
     2641 ttctcttttg ttccttagtt ggtatgatag gtaccaaatc aagttcatta tttacttttc
     2701 ctgtgctgct ttcgctgctc gtgcttcctg tgctactctc actactcatg cttcctgtgc
     2761 tactttcact actcgtactc tccgtactgc tttcgctgct cgtgcttcct gtgctacttt
     2821 cactactcgt actctccgta ctgctttcgc tgctcgtgtt ttctgtgcta ctttcactgc
     2881 tcgtactctc tgtactgctt tcaccgctcg tgttttctgt actgctttca ctacttgtgc
     2941 tatccgtact gcttggcggt ccaacaactg tccaactgct tggtttcaaa ttctccgtga
     3001 tcgtcatctg tccatcataa aaaacaatca aatcaacttt atctttactc ttgatctcat
     3061 caatcgatag ttctgcagaa atcattattg tatactcaat accagcggtc cattgtacgt
     3121 cttttaaata aacttcccca ataacttttt ctgaacttcc attaccatcc caaccatatg
     3181 ttagaacagc atgtacatca ctagcattca cgcttttgtt tggtttgact ggaatagtaa
     3241 tgacttgttg tgtcatgtca taaacaatcc gatcagtttt cacagtaaat gaaagattct
     3301 catttgcccg ttcatttgca gaagtactga ttccaatcat tgctgcaatt atactcagcc
     3361 caagaataac aaataatacg gtcgctgttt tcttcatttc tatcactctc catataatta
     3421 ccttctattt gataaaggaa ttatataaaa tgaaaatgat gagaacacga cagtcagttg
     3481 tgaaaatcta acaaaagatg tcgtgtattc gcttgaattt gtttactttt tcgtttaatc
     3541 aatgacagac ttaaaaaagc aagaccgata tcagccttgc tttcttcatc ttttattgat
     3601 aaacagcagt gtatttcttc atccgctttg ttttatatgc atagagtaac aaaccaacaa
     3661 tcaagactaa caagaataat gtagttgata tagatgcttc agccccaaag tttcccccag
     3721 taattattgt attttgcgat tggatcagag tggagaaaaa accttgttct tgcgttccgc
     3781 tgactggtat acccaaaata gctccttgtg tccaattcca agtaatatga gcaccaatag
     3841 ccgtaaataa acttcctgta ctgtagataa gtgcgctgaa aaagcaacct gctaaaaaga
     3901 tattgaaaac tgccagaaat gtcatgttag gattcaatcc gtgcaaaaga gcaaataatg
     3961 cagcagatcc aatcattgct attaacggac caaatctaag catctgatgc tgaaaaaagc
     4021 cacggaataa aagctcttct gatgccccca ctgcaataaa taaaataaaa ctcgttatca
     4081 gcggcacaat gtttgcgttt cctgaatcta accgatacgc ccccgtcaaa tataatggac
     4141 tagtaataag cagaaacact ccaattccaa caacgagacc tcccgctaaa tacccccagt
     4201 cttttcctcg aacagaaaca ccataatctt ctattctttt tccttcgacc ttactagcaa
     4261 gaaataaagc tagtaaaaaa acagctaaat gtccgaccgt accggcaatt gctccaatcc
     4321 attcactatt tccaataaac atatccgcaa gtgcattatt gacaagaatt cctaaaacaa
     4381 aaccaactaa gactattaaa acagatccaa tcaaccgaat aatcctgatc atctttcatc
     4441 cctcctttat attaagttta acaacccttt tctttcttgt ctcgtgaaaa ctatgtaaat
     4501 gttctccatc atagagagaa tagattttta cgaactaaga atatctacag caaccttacg
     4561 aaaatgaaag actgtctccc taattcttct gttagactgt ttttaataag gaaaggatgg
     4621 gaaatttttt gaaaaaacaa ctactaatta tgtgcctttc agcatgtgca attttactat
     4681 tctcaggttg tagcaagtcc aatgaaacga ttgcaacgtt taaaggtgga acacttacag
     4741 ttcaggattt ttatgataac ccaaaagtta aatcaaataa tcaaacacta ttaaaaaaaa
     4801 tgattgtctt caaagcattt gatgatgtat acggaaaaga gatttcttca aaggaagtaa
     4861 caaaagaata taatgaacaa atcaaaaaac tcggtccaaa ctacaaggaa caattaaaag
     4921 cagttggcca aacagaagag acatataaga aactctttaa acagatgctg gctttccaat
     4981 acggtttgaa agccaatgta aaattaacag ataaagatct agacactgct tggaaagaat
     5041 tctatccaga agttagtaca cagatcattc tattttcgac taaagaagaa gcagataaag
     5101 caaaaaaaga agcaaatgaa ggggaaaatt tctccaagct tgttcaagct tatggaaaaa
     5161 ataaaacatt aaaggaaact gatggaaaaa tgaattttga ttcaaccaat ccagagattc
     5221 ctactgaagt aaaaaaagca gcatttaaac taaaaaacgg ggaagtttct gatatcattc
     5281 ctgtaacaga tccaacgact tatcagcaaa gctattatct agtaaaaatg gtaaaaaaac
     5341 aggataaagg ttctaataaa gacaaataca aatctgagct agaaaaaatt gctaccgaag
     5401 caaaaactct tgatacggaa ttcatggata aaacaatccg taaagtgatg aaaaaagaca
     5461 atgtaactat taaagatcct tatgtaaaaa atatattcaa ataaacaaat ttgacccaat
     5521 tggggatgtg gcaaaatttg agaagtgatt tttggaaatt ccttcttatt tttgaggcaa
     5581 accacttttg ccacaacttc atcggggcct ttttttattt gtaccaaaac aaatcactgg
     5641 atttgttaca atcatgagga tataatttaa aaaagttttt tatttctatc acatgaatca
     5701 atgcgccatt aagtagattc tttcatgctt ccctattttt tcacttgccg tatcgcttca
     5761 aaaacaacta tcataaactc aaactcgact gaaaagttat tttaaaaaag actaaaaata
     5821 tcccttctgg acttgttcct cttattctgt tatactagag aaacacgaaa gatattttta
     5881 tgcgacactt ttcatttgtt tttatgaaat ttattcaaaa ctggcgtaac ttcagagtta
     5941 caaggatgta ttagaggtta gggaatacat cgtttaaatg agaaataagt aatagtcacc
     6001 caaactttaa attaatgaat attgaatcgt cagatagcct cctcgagttt tcgagaaggc
     6061 tatcgtttat tttatggaga aattgattca gaagtaatca gcgacctttt ttagttcacc
     6121 aatcgtttct gcttggaaag tttgcaccca atcttcttgg aagcccctca ctgctttatc
     6181 aatcgagggc atctctaata cttgatctag tagctcttta cctaacgtta tcgcatgagc
     6241 tccagatcga atggctgtag tgacttgttc acaatttttg aaactagctg ctaatatttt
     6301 tgttggtctt tgcgtacgtt cgatctcagt ccgcaacgaa tcaatcactt tcatagcatc
     6361 aatgccttga ttttgcatcc tgttataata aggtgcaata taatctgctc cattattaat
     6421 agctaaatac ccttgtaatt ctgtgtaaat agctgtaccg gtaatcaaat aaccttcttt
     6481 tttcaacttt ttgatcgctt gaaggcctgc ttgattcacc ggcaccttga taaacgtatc
     6541 ttttcccagt tcttctatga taactcgagc atcctctatc atttcttctt ccgttcttcc
     6601 tattgtctga atatggatag gaatctgtcc gattattttt ttgatttctt tcatatgcga
     6661 gaagaagttt gacgctcctt cttttttcag aattgtagga tttgttgtta cgcctgttaa
     6721 attcaaaaac tgttgacaag aataaatact ttttgtattt gctgtatcaa gcatgatttc
     6781 catatagctt tcccccattc tgatcctctt gatcgatttt aatgattgtc ccctttctta
     6841 aatgcggcat ttccttatct tctacataca ttgttcctgg aagttccggc tcatgagatc
     6901 catcaaattt tatcgtgata tgtcctaatt cattcaaatt agtttggaca acatctccta
     6961 ctgctgtgat tacatacatt tgtttatcaa aacagagctt catatcacaa gttatttctt
     7021 ttcttacctg attcaattca atcttataac aataatctgt taatgt
//
LOCUS       DACEZN010000134         7041 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEZN010000134 DACEZN010000000
VERSION     DACEZN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.134"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(28..726)
                     /locus_tag="IXH60_002258"
     CDS             complement(28..726)
                     /locus_tag="IXH60_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1991151.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="IXH60_002259"
     CDS             complement(719..2071)
                     /locus_tag="IXH60_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1991152.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="IXH60_002260"
     CDS             complement(2294..3694)
                     /locus_tag="IXH60_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1991153.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="IXH60_002261"
     CDS             3855..4592
                     /locus_tag="IXH60_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1991154.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="IXH60_002262"
     CDS             4717..5196
                     /locus_tag="IXH60_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1991155.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="IXH60_002263"
     CDS             5193..5960
                     /locus_tag="IXH60_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991156.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="IXH60_002264"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="IXH60_002264"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1991157.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
BASE COUNT         2374 a         1328 c         1306 g         2033 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg t
//
LOCUS       DACEZN010000135         7008 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEZN010000135 DACEZN010000000
VERSION     DACEZN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.135"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..116
                     /locus_tag="IXH60_002265"
     CDS             <1..116
                     /locus_tag="IXH60_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1991158.1"
                     /translation="NYKTGENYAYLGLPPNFLIFDEYVAYMGANRFPTSIE"
     gene            710..2584
                     /gene="ltrA"
                     /locus_tag="IXH60_002266"
     CDS             710..2584
                     /gene="ltrA"
                     /locus_tag="IXH60_002266"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1991159.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            2656..3030
                     /locus_tag="IXH60_002267"
                     /pseudo
     CDS             2656..3030
                     /locus_tag="IXH60_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346898.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3125..3800
                     /locus_tag="IXH60_002268"
                     /pseudo
     CDS             3125..3800
                     /locus_tag="IXH60_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            4033..5175
                     /gene="dcm"
                     /locus_tag="IXH60_002269"
     CDS             4033..5175
                     /gene="dcm"
                     /locus_tag="IXH60_002269"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1991160.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AALLRPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYIPQSRERI
                     FLIGHSRDACTEQVFPIIGPSSTSDQNIRNLVNINPSNRGMGGQVYGSDGVAPTLTGD
                     EGIKIALPARDGISVAGLLPGKFEQGNRVYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAIVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVQKCDL"
     gene            5165..5455
                     /locus_tag="IXH60_002270"
     CDS             5165..5455
                     /locus_tag="IXH60_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991161.1"
                     /translation="MIYDELVGEIYWVIEKIQSDPELEEQLRRLNFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            5713..6897
                     /locus_tag="IXH60_002271"
     CDS             5713..6897
                     /locus_tag="IXH60_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1991162.1"
                     /translation="MVQQNLDYRLLKDRRNQYGVSQNKLATACGLSRPYLNQIENGGV
                     TTSTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVQKIVQEILHLKFDYMLHED
                     YAFYSYQEQYIMGDIVVMLSHEEEKGVLLELKGRGCRQFETFLLAQKRSWYDFFDDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDKSETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIEGTTFSIINRYLRFTDKVEGKRRTNWPLNERWGRFIGRNRKEIQLTSDPKPYT
                     IERTLSWLGRQVAPTWKMAKELDRLNQTTYILDMVQNAQLSDRHKKILEQQSMAIENL
                     II"
     gene            6894..6992
                     /locus_tag="IXH60_002272"
     CDS             6894..6992
                     /locus_tag="IXH60_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1991163.1"
                     /translation="MKRGSSMLGITVMCLIHTSAAADRVMERKVIK"
BASE COUNT         2351 a         1172 c         1520 g         1965 t
ORIGIN      
        1 ctaactacaa aacaggagag aattatgcct atcttggact tccacccaac tttttaatct
       61 ttgatgaata cgtggcttat atgggtgcga atcgttttcc tacaagcatc gaatagatgt
      121 gaaatggaag cctattgaga ttatcaatag tgacaactaa tgttttgaga gatggaatga
      181 aagccttcaa gttgagctga aacatccccg ctaatactcc tacatgctct aatcaatgaa
      241 gtagattggt tttggtatga agctaggtga agtcgtctga aatctgccta agtgcttgag
      301 cataaggctg actatagggc gggcggctga aaaagataac tatggtaaga atgtctgaaa
      361 atgactgacg aatctgcgaa tgtacgggtc taaaaggctg atcttattga aagataggaa
      421 catacggacg tatggtgaga taccgacaag taaggttcca tgatatgaaa atcgggatag
      481 ctaacgaaga agctataatt tcacaggctc atagtagata cctaaggtta tacatgatag
      541 ctaaaaacat tggaacgatg taagcagaaa actatcaatt aaatgactac ttctagatag
      601 gtgcatataa gtaataacga aattgctttg tttctgtgaa agtaagggca cagtaccgat
      661 gaagcgtcta acaaatgtgg agggatagcc ctaagtctat ttaataacaa tgaatgttga
      721 aacagatgaa gttcaaggat ctggtaagaa cgtgggaaaa tccgttatgg aggtgccacg
      781 cttgaacaca aaaatacgtt actgggaata ttatgatcta caagaaacat tcgatcagct
      841 atttactcaa agtagaaacg ggaaaaagtt ttatcaatta tatgagttaa tcatttctga
      901 aaataatatt cttttagcat accgtactat caaggcaaac aaggggtctt cgacaccagg
      961 aacggattcc ttcacaattg ataactataa ggaaatgaac caagcagagt ttatccatct
     1021 tattctcagt catttagaaa attacaaacc aaaatccata aagcgagtaa tgatacctaa
     1081 acccaatggt gagaaacgcc cattaggaat tccatgcatg attgatcgaa tgatccaaca
     1141 gatgttcaag cagatactgg agccaatatg tgaagctaag ttttatgaac atagctacgg
     1201 tttcagacca ttgagaagtg caaagcatgc tctaggtcgc attatgtatc tcataaacat
     1261 cagtaaaatg cactatgcag tagacattga tatcaaaggt ttctttgata atgtaaacca
     1321 ccgtttactg ataaaacaat tatggaatat aggaatttgt gacaaacaag tcttagctat
     1381 tctttcaaaa tctctgaaat cacccattca aggagaaggt atcccgagca aaggaacgat
     1441 ccaaggaggt atcatttctc cactattatc taatgttgtt ctaaacgact tagaccattg
     1501 ggtctcaaag cagtggcata cctttgaaac aaagtatccc tatactaaag gttacaataa
     1561 atttcgtgct ctaagagata caaatctaaa gcaaggctat attgtcaggt acgcagatga
     1621 ctttaaaatc atgactaatg attatccgac agcattaaaa tggttccacg ctgtcaagct
     1681 ttacctaaaa gacagactta aattagatat ttccaatgag aagtctaaaa tcgtcaattt
     1741 gcgtaaatgt aagtcggaat ttttaggatt cgctatttgc gtgaaacaaa aagggaaaaa
     1801 atgggtttgt aattcacgta tttctaacaa aaaaaaggat cagattaaag aagagattaa
     1861 acaaagaatc aaagatattc aaaagagtcc tacagcacaa aatgctctcc tgtttaatgc
     1921 tctcgtgctc ggtgttcata attatttcag gtatgccact catattaatt tagatttgaa
     1981 taggatgaac tacgttcttt cattcactac ttacaaccga ttatcaaatt gtagtacaag
     2041 atgttacccc aacaaagctt ccccaactta caaaaagttt tactcaacaa cgatgaaaac
     2101 gtataaagta gctggagtct atttgtaccc tctttgcgac gtatctacaa aaacaattta
     2161 cggatttaat acggaggata caccgtttac tccgtttggt cgtcaaagat tggagcacaa
     2221 aagcctacag tctcttgtgt tccaagaatt aagaaaattg atggaatcga aaataccgaa
     2281 tagaacggtt gagtatttag atagtagaat atctcgttac tcaatgaaat caggcaagtg
     2341 tgagattaca aaacaatttt tgccagcaaa agccgttcac tgtcatcact atttgccaaa
     2401 aagtcttggt ggagacgaca aattcgataa cttgagaatt attcataagg atatccatct
     2461 tctgatccat acaacaaata aaatgataat cgatcactac gtgcatgaac tgaaactggt
     2521 gccagagcaa atagcaaaaa tcaaccttta tcgaaagatg tgtaatctgc aaaacattca
     2581 atagaaaata aatagatgga acgccgtatg aggtgaaagt ctcacgtacg gtgtggagcg
     2641 ggggaaaaga tggagattat atcaaattct tacctatcgc tatagatgtt aacgacgaaa
     2701 gaaagtgcgg tgattttgaa taagctcaaa caaatcgtga tgttgggtcg tcaatctggt
     2761 ttctttctga ttttggcttg ccaaaggccg gatgcgaaat atttaggaga cggaatccgc
     2821 gatcaattca actttcgcgt ggctttgggt cgtatgagtg aactcggata ttccatgatg
     2881 tttggggagg tcgaaaaaaa cttctttatg aagcacatca aaggtcgagg ttatgtggat
     2941 acaggtggca gtgtgattag tgagttttat acgccacttg taccaaaagg gtatgatttc
     3001 ttagattcaa ttagtaaatt agtaaattag aaaagatgaa tgattgagtg atcttcaata
     3061 ctgactttat acttcaagta acgtataata cttttatata gtaggaagaa aggggataat
     3121 taatatggaa agtaatgatt cttatttaga gtatgcaaaa gcaaataagg ataagtttat
     3181 ttcttatatt atcaaaggga aatcccccga agttggacga gacgcagttt ttatggcagg
     3241 gagtcctgga gctggaaaat cagaggttgc cattggatta gctgagaatt atgataacca
     3301 tgtgattatt gatgcggatt actttcgcac ccagtttcct gattataatg gaaaaaaact
     3361 ctagtctctt tcaaaaagca tcttcttggt tggtagagca ggcatttaga tatgtcttag
     3421 atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct gaaaaaaata
     3481 tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctacgtttat caagacccta
     3541 aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat gtgccgaaaa
     3601 aggtatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta aagagtaaac
     3661 atccagatgt attgcttcat atcataataa aagattatac gaataatatt tccgaagttc
     3721 attatgctgc ggacaatcta gaattatttt taccactagg gtatagtagg aaagatttgg
     3781 aggttcaatt acatgactga taaagaacaa gtaacgaaaa ttgtaaagaa atacaataag
     3841 agcattgcgg atttatcgga aaatgctact gcaaaagagt tcaaaacggt gattaaatat
     3901 gtagccgatc aggccaatga aaaacaacgg aaattagttg gattaaataa aaaatagtag
     3961 gacaaattaa taatgtatga gccaagagca aaatgttctt ggcttttttc tttgcaatga
     4021 atgaggtaat gtatgagatt tttagattta ttcgccggta ttggtggctt tcgtttaggt
     4081 atggaacagg cgagccatca ttgtatcggt ttttgtgaaa ttgataaatt tgctagacgt
     4141 agttataaag caattcatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta
     4201 tcagatgagt ttattcaatc cctcggacca gttgacatcc tttgcggcgg atttccgtgt
     4261 caagcttttt caattgcggg aaagcgacaa ggattttccg atactcgagg tactttattc
     4321 tttgaaatcg ctcggttcgc cgctcttctc cgacctaagt ttttattcct tgagaacgtc
     4381 cggggattgc tcaatcacga agggggggct acgttcgaga cgatcctccg aacgctggat
     4441 ggattggggt atgacgtgga gtggcaggtg cttaactcaa aggcctacat tccccaaagc
     4501 cgggagcgta tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct
     4561 atcattggac cgtcttcaac atctgatcaa aacatcagaa atttggtaaa tatcaatcct
     4621 tccaatcgag gaatgggtgg acaagtctat ggttcagatg gcgtagcgcc aaccttaaca
     4681 ggtgatgaag gaatcaaaat tgcactgcca gcaagggatg gaatctctgt agcaggacta
     4741 ttaccgggaa agtttgaaca aggtaatcga gtttatgaag ttactggaac cgcccccacc
     4801 ttgtccacga aacaaggtgg tacaaaaatc atgattcgaa aaaatgaaac agaatatcaa
     4861 gaggtgaagc caggagacag tgtgaacctg gcttttccta attccacaag tcgccgaggt
     4921 cgtctgggaa aacagagtgt tcatacttta ctgacaggag accaacaagc gattgtcacg
     4981 gatcagtatc aaattcggaa gctaacacct agagagtgtt ggcgacttca gggctttccc
     5041 gattgggctt ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaagcc
     5101 ggaaactctg taacagttcc ggtcattttt gatattgcca gaagattaaa ggaggtacaa
     5161 aagtgtgatt tatgatgagt tggttggaga aatttattgg gtgattgaaa agattcagtc
     5221 agacccagaa ttagaagaac aattgcgacg gttaaacttt gatattcgca agaatggtgt
     5281 caaagtcgct ggcgatccat atttaatgaa tgaggagacg gatgctcgaa ttgaaataaa
     5341 ccaagtaatt gcagaatttg agcggattgc ggaccaagca aaagaaccgg atattcgtca
     5401 atacttgtta gaaatgaaag cagagttgga agtaaatggt attacagatg agtaacctaa
     5461 accaacacaa ctgactgtta ggcgctgacc gcggaggatt cgcgaagcag aaatcctccg
     5521 tggtgctgtg cctgttcatg gacggcgtta gccggccatg atgtctcacc ccccgtatct
     5581 aacagggggg tacaaaaaca caaaaaacaa gcaagcagcg attaagaatg gttgtcatga
     5641 cgaggatagt tagctgtttg aacaggtgtt aactcatttt gaaaagttaa caccttattt
     5701 ggaggtgaaa acttggtaca acagaactta gattatcgtt tactaaaaga tcggcgcaat
     5761 caatatggtg tttctcaaaa taaattggct actgcttgtg gattgagtcg tccctatttg
     5821 aatcaaatcg aaaatggtgg cgtaactaca tctacaaaaa ctatgagaaa aatttttgac
     5881 caactggaaa gtttcaatcc cgatttgcct ttgacgttac tgtttgatta tgtaaggatt
     5941 cgttttccga caacggatgt tcaaaaaatt gttcaagaga ttttgcattt gaaatttgac
     6001 tatatgcttc atgaagacta tgccttttat tcgtaccaag aacaatacat catgggtgat
     6061 attgtggtga tgctttctca tgaggaagag aaaggcgttc ttttagaatt aaaaggtcgt
     6121 ggttgtcggc agtttgaaac ttttttgctc gctcaaaagc gtagctggta cgactttttc
     6181 gatgattgct taaaagctgg aggcgtcatg aaacgcttgg atttggcaat caatgatcga
     6241 gttggcttat tggatattcc tgagttgacg aaaaaatgtc agaaggaaga atgtatctcc
     6301 ttattccgga cttttaagag ttaccgctcg ggtgaacttt tgaaagctga tgaaaaggat
     6361 ggaatgggaa atactttgta tatcggaagc cttaagagtg aggtttattt ttgtttatac
     6421 gaaaaagact atgaacaata tatcaaattg ggaattccgt tagacaagag cgaaacgaag
     6481 aatcgctttg aaattcgatt gaaaaatgac cgtgcctacc atgcaataca agacttgtta
     6541 aaaggtcgaa gtattgaggg taccacattt tccattatca accgttactt acggttcacg
     6601 gataaagttg aagggaaacg gcgaacaaac tggccgctaa atgaacgatg gggtcgcttt
     6661 atcgggcgaa atcgcaagga aattcaactg acttctgacc ccaaacctta tactatcgag
     6721 cgaacactga gctggctagg tcgacaggta gcacctacgt ggaagatggc taaggagtta
     6781 gatcggttaa atcagacgac ttatattctt gatatggtac agaatgctca gctatcggat
     6841 cggcataaaa agattttgga acaacaaagt atggcaatag aaaacttgat tatatgaaaa
     6901 gaggaagcag tatgcttggt attaccgtga tgtgcttaat tcatacaagt gcggctgcag
     6961 atcgagtaat ggaaaggaaa gtgataaaat gaatttcgga caaaattt
//
LOCUS       DACEZN010000136         6975 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEZN010000136 DACEZN010000000
VERSION     DACEZN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.136"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            203..1144
                     /locus_tag="IXH60_002273"
     CDS             203..1144
                     /locus_tag="IXH60_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1991164.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1213..1944
                     /locus_tag="IXH60_002274"
     CDS             1213..1944
                     /locus_tag="IXH60_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1991165.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2088..4268
                     /locus_tag="IXH60_002275"
     CDS             2088..4268
                     /locus_tag="IXH60_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1991166.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4288..4770
                     /locus_tag="IXH60_002276"
     CDS             4288..4770
                     /locus_tag="IXH60_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1991167.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4786..5547
                     /locus_tag="IXH60_002277"
     CDS             4786..5547
                     /locus_tag="IXH60_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1991168.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5678..>6975
                     /locus_tag="IXH60_002278"
     CDS             5678..>6975
                     /locus_tag="IXH60_002278"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1991169.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAY"
BASE COUNT         2341 a         1224 c         1540 g         1870 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg tttttctgtg cgaaacagga
       61 aagcgaagtc tttttaattc aaataataat attatgtaaa caaaaaactt tccatgcgta
      121 attatacaca agcaaataaa tcgtcaaatg cgaacgcctt ctttatactg tttaggaacg
      181 aaaagaagga aggaagaaat acatggatta tcaagtactg ctttattaca attatacaaa
      241 aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc ttttgcaaat ctttggatct
      301 taaaggtcgt attctagtag ctgaagaagg aataaatggt actgtgtctg gaaccgttga
      361 aaacacgact gcctatatga aagcaatgaa agctgatcct cgatttgctg atacgatgtt
      421 caagatcgat ccaagcgaag gacatgcctt taagaagatg tttgtaagat accgtccaga
      481 gcttgtaagt ttaaagctag aagaggacat tgatccaaac caagtgacag gtgcctatct
      541 ttcaccgcaa gaatttaaag aagctatttt agatgaacag acagtggtga ttgatgcacg
      601 gaatgattat gaatatgact tgggacattt tcgaggggct gtacgcccag atatacgcag
      661 ttttcgagag cttccagact ggatccgcga gcacaaagaa gaatttatgg aaaaacgggt
      721 agtgacctac tgtactggtg gcattcgttg cgagaagttt tctggttggc ttgtccgtga
      781 aggattcaaa gatgtcggac agctgcatgg cggtattgct acttatggta aggatcctga
      841 agtccaagga gaattatggg acggcaagat gtatgtgttt gatgaacgga tcgctgtcga
      901 tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg tttgacggaa caccatgtga
      961 acgatatatc aattgtagca atcctgagtg caatcgtcag attcttgctt ccattgaaaa
     1021 tgaagaaaaa catttaggag catgtagcat agagtgtgcg aaacatccgc gcaatcgcta
     1081 tgttcaagac catggaatct ctccagaaga agcagaactg cggtttacag ctttaattaa
     1141 atagaaaaca atagttttga ttgtcgacca tttccaatat gcgctatact agcgttgagg
     1201 atggtgatcc acatgaaatc attgatttat gctattgggg atgtccatgg caaacatgag
     1261 ctttttgaaa aacttattca ctattatgat ccaaagatcc atcagcttgt tttattaggg
     1321 gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt tattaggaaa gcaattagtc
     1381 gaagaaacag gagccattta cctccgggga aaccatgaag aatatttttt gcaatttctg
     1441 caatcaccgg aggactggta tgcgccttat atgagaaatg gtggaaaaga aacgtttgaa
     1501 agcctgattc acccaggggc atcggaggaa tactccccaa ctgaaatctc gatgatgatc
     1561 caatcgagat attcagaact gattaaattt ttagtccagc gccccttgta ttatgaatgg
     1621 gaaaattatc tttttgtcca tgccggtgtg gatttgacaa agaaagactg gcgtaagaca
     1681 agtctaagag actttatttg gataagggaa ccatttcatg aagggaaaaa caatacaggt
     1741 aagacaattg tatttggaca tacgattaca ccaatgctcc atggagatat gcagacaaca
     1801 gacctctgga ttcaggatca taaaatcgga atggacggtg gggctgtatt cggagggtca
     1861 ttgcatgggg ttgtttttga caaggaaggt attcttcaag atattgaata tcagaacaac
     1921 gaaggacctt ggcatggaat ctgactaatg gaaagaatga aggagctgtg actttgttgc
     1981 ggctcttttt atttttatag agaatattgt ttctttcagt agtttaaccc aggataaaaa
     2041 gtatgataga ataaagaagc aatttttagc agaaaagagg cattttaatg acggaaaatt
     2101 tgaaccagac gatcattcag cttgtacaaa aagaactttc agactatcgt cctaagcagt
     2161 tgactactgt acttaatttg ttaaatgaag gaaacactgt tccttttatc gcgcgttacc
     2221 gaaaagaaat gactggaagt cttgatgaag tccagattcg tgaaatcgaa gagcggtatg
     2281 cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat tcggctaatc gatgaacaag
     2341 ggaaactgac tcccgagtta gaagcagaaa tcacccaagc cgtgaaaatg caacaagtgg
     2401 aagatttgta tcgtccatac aaacaaaaac gacggacaaa agcaacgatt gctaaagaaa
     2461 aaggacttga accactcgcg aagtggctga tgcagcttac agatggagag gtgcaatctg
     2521 aagcagaaaa atatattgac aaagaaaaag aagtctcttc tgccgaagaa gcgttgcacg
     2581 gtgctcatga gattattgct gaacaagtca gcgataatgc aaaatttcgg acttggattc
     2641 ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt caaggatgag caagcagatg
     2701 aaaaaggcgt atatgaaatg tattatgact ttgcagaacc cgtccataaa atggtttctc
     2761 atcgaatatt agctactaat cgtggagaaa aagaagatat tctgaaagta ttctttcaag
     2821 tagatgaagc agcaatttta gcttacttgg atcgccaaat agtcaaaaat cctgcctctc
     2881 cttcttcttc attcgtcaga gaagcctatc aggatagtta taaacgcttt attcagccag
     2941 caatcgagcg agaactgcgc aatgaattga ctgaaaaggc agatgagcaa gcaatcgcta
     3001 ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc actaaaaggt aaagttgtcc
     3061 tgggatttga tccggcttat cgtactggat gtaaactagc agtcgtagat gcgacaggga
     3121 aagttttagc aatcgaagtt atttatcctc ataaacctgc agcgcaagca aaaagagaag
     3181 ctgctggacc agcttttatc caattaatta ataaatatca agtagatatg gtagctatcg
     3241 gaaatggtac agccagtcgg gaatctgaac tttttgtggc tgagcagttg aaatccgccg
     3301 accataaaac ctattacgct atcgtcaacg aagcaggcgc ttctgtttac tccgctagcg
     3361 aaattgcacg aaaagaattt ccgcatctac aagttgaaga aagaagtgct gtttctattg
     3421 cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat cgatcctaaa gctgtcggag
     3481 taggccagta ccaacatgac gtttctcaaa aacgtttggc tgaacaattg gattttgttg
     3541 tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac agcaagcccg cagctcttgc
     3601 agcatatatc aggattgaat aaaacaactg cccaaaacat tgtcatttat cgtgaagaaa
     3661 acggagaatt caccgcacga actcaattga aaaaagtgcc acgcttagga ccaaaagcat
     3721 atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa aaacattttg gacaacacag
     3781 gtatccatcc cgaaagttat tctattgcaa aagaaatatt aacttcagtc caactttcag
     3841 aaaaagagct tggtacagaa gaagcagtag aaaaattgac acgattatcc gtagagaaac
     3901 tggctgaaag cctatcagtc ggagaagaaa cgttagccga tattttagct gggctgacac
     3961 aacctggacg agatatgcgt gatgagatgc cagcgccctt attacgtaca gatgtattga
     4021 gtatggaaga cttgaaacca gggatggaat tgactggaac ggtccgtaat gtcattgact
     4081 tcggcgcatt tgttgatatc ggtgtgaagc aagacggact tgtccatata tcgaaactca
     4141 gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt aggagatatc gtaacagttt
     4201 ggatagaaca agtcgatacg aaaaaaggca gaatcagttt gacgatgctt tccccttatg
     4261 aagagtaaca atgaggagta acaaaaattg acacaagaag aactgcagca gttagtagaa
     4321 gagatatcat taaaaagctt ccagcttcct ttcaagcacc aagcaatgtt caatccacgc
     4381 ttacgtacaa ctggcgggcg ctatcattta aacgatcatc atttagattt taatgcgaag
     4441 ctatttgaag agtcagacga agagacgatt gcaggaatca tcaaacatga actttgccat
     4501 tatcatcttc atctagctgg aagagggtat cagcataaag atatggattt caagtatctt
     4561 ttgaaaaaaa caggagggtt gcgctatgca ccagccttga cgaagaaaca gataaagatg
     4621 gagtattatc gatgccaaga ttgttctgcc acgatctgcc gcaagagaag aatcgatgta
     4681 cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat ggattgagac aaaaagagaa
     4741 gactttaatg aagcacaaaa aatcagatga aaggatagaa ggcctttgga aaaaagtgag
     4801 cagaggaatc taaaagactg gaatcagcta ttaaattttt tagccgcacc tgaagaggga
     4861 aattttgagg aactgacggc acctacactt attttagctg gaaactgtct tcctatctta
     4921 gctgataaag cagcacaaat gtatttaaat ggccaagtag atcaattgtt tttggtcgga
     4981 ggggtggggc atgcgactag aatcctttat gagaattttg aaaagcaagg atttcatttt
     5041 gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc taaaagaagt ctatgatttg
     5101 cccgataagg cttttctgat agaatcgaag tctacaaatt ctggagagaa cgccactttt
     5161 tcactggaga ttcttcattc cttaggagct gtaccagaaa aaatcttatt gatgaatgat
     5221 ccgacactgc aacgcaggac tcgagcaact tttgagaaag tatggcaaaa tgaacaaact
     5281 attttcgcga atgctgtgcc atttgtgccg gaaatccttc acttttctga agagatcatc
     5341 tttacagcaa aagaactgaa tcatcaatgg cctaaggaat atttccatgc gctagtatta
     5401 ggagaaatgg aacgtctcca tgacgatgag aacggctatg gtccgaaagg aaaagatttt
     5461 attcctcaca ttgatatatc agaggaagtt tggtcttctt acaccagaat taaagagtca
     5521 ataaaaacgg atttttctcg tacttgacca ttttagcaag cgaatcctcc tttccaaata
     5581 aatttggttg aaattttcaa tcgtttataa gataatgagg agcgtgttga atgtaatagc
     5641 taaaagaatg gacgctagat aagagaagag gattcaaatg gaaaatcaaa ataaattgta
     5701 tcattttgtt ggtatcaaag gttcaggaat gagctccttg gctcttgttt tacatgaaca
     5761 aggtctaaat gtccaaggat cagacattga aaaatatttc tttacacaaa gagatttaga
     5821 aaaagcaaat atcacgattc tgccatttaa cgcagataat gtaaaaccag ggatgacgat
     5881 cattgcagga aatgcatttc ctgattcaca tgaagaaatc cagcgagcaa aagaattagg
     5941 tttggaagtg attcgctatc acgactttat tggtcatttt atccaaaatt atacgagtat
     6001 cgctgtaaca ggctctcacg gaaaaacaag tacgactgga ctgctctctc atgtattgag
     6061 tggtgtccgt ccaacaagtt atctaattgg agatggaaca ggacatggtg atccgcaagc
     6121 ggaattcttt tcatttgagg cctgtgaata tcgccgtcat ttccttgcct attcaccaga
     6181 ctatgcaatc atgaccaata tcgattttga tcatccggat tattatacaa gtatcgacga
     6241 tgtatttaca gctttccaaa caatggctgg acaagtgaaa aaagctattt ttgcgtatgg
     6301 tgacgatgct tatttacgca aattaaaagc aaatgtgccg atttattatt atggtgtcac
     6361 agaaaacgat gatatccaag cacgaaacat cgaacgcaca acttctggtt ctgcttttga
     6421 tgtctatcat ggggatgaat ttgttgggca cttcactgta ccagcattcg gtaaacacaa
     6481 tatcttaaat gctttaggtg ttattgcagt agcttatttc gaaaaactgg atctgaaaga
     6541 agttgcagaa gaaatgctga ctttcccagg agtaaaacgt cgattcagtg aaaagatcgt
     6601 agcagacatg accgtagtcg atgattatgc gcatcaccct gcagagatca aagcaacgat
     6661 tgatggcgca cgccaaaaat atccagataa agaaataatt gcagttttcc aaccccatac
     6721 gtttacgcgt acgattgcat tgatggatga atttgcagaa gcacttgatt tagctgataa
     6781 agtttactta tgcgatattt ttggttcggc tcgggaagaa caaggaaacg ttaagattga
     6841 agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc aaagaaaata atgtatctcc
     6901 attgttagat tatcatgatg cagtagtaat cttcatgggt gctggagatg ttcaaaaatt
     6961 tgaacaagcg tatga
//
LOCUS       DACEZN010000137         6924 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEZN010000137 DACEZN010000000
VERSION     DACEZN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.137"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(156..620)
                     /locus_tag="IXH60_002279"
     CDS             complement(156..620)
                     /locus_tag="IXH60_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1991170.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(642..962)
                     /locus_tag="IXH60_002280"
     CDS             complement(642..962)
                     /locus_tag="IXH60_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1991171.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(1105..2184)
                     /gene="pepA"
                     /locus_tag="IXH60_002281"
     CDS             complement(1105..2184)
                     /gene="pepA"
                     /locus_tag="IXH60_002281"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1991172.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            2384..2734
                     /locus_tag="IXH60_002282"
     CDS             2384..2734
                     /locus_tag="IXH60_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1991173.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            2919..3872
                     /locus_tag="IXH60_002283"
     CDS             2919..3872
                     /locus_tag="IXH60_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1991174.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            3885..5672
                     /locus_tag="IXH60_002284"
     CDS             3885..5672
                     /locus_tag="IXH60_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991175.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            5659..6861
                     /locus_tag="IXH60_002285"
     CDS             5659..6861
                     /locus_tag="IXH60_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991176.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         1995 a         1282 c         1248 g         2399 t
ORIGIN      
        1 aaaaaattgc attcactgca tgagattttt ttcatgcttt atttccgaag aagcttcttc
       61 tggaacacca tttattcgta acaagggact gtgacaagac ttttgtcaca gtccctttac
      121 tgtttgatta aaaaggttat acaagttcca cttgcttaat tactcggctg ttcttcttcg
      181 tcagaaacga tcaagacatc acaaggtgaa tgacgtatga cgtagctggc tacacttcct
      241 gtcataaagc gttcgactgc atttagtcca gactgcccaa ccatgattag atcgattgca
      301 tatttttttg gcaatttgac tgccatggat tcacgaacag agccgtatgc aataattcct
      361 tctatatttg tgtaatcaac agattttcca tattctttac attcttcgat catttgcttg
      421 gctgtttcag tttgctgatc gactagattt tgatcaatag gagcatagcc cataaaatta
      481 taaaattgct gatcgattac gtttgctaca tatactttcc cattattacg gcgagccact
      541 tctaacgctt ttttgaaagc agcaagtgct tgttcactac cgtcaatacc tactaaaatg
      601 ttcttatatt cttgattcat cttgacccct cctaacagat gtcagccttt tattacactt
      661 tctaggaacg attcgatttc ctgttttgtt ttacgatctt tattcactag acgacctaat
      721 tcttttccgt cttggatcac gacaaaactt gggataccaa aaatgttcca ttcagcggct
      781 acatctatgt acttgtcacg gtctacttca ataaattgcc aatcagaaaa gtcattttcg
      841 atttcaggca tttgcggttt gataaaacgg cagtctccgc accagccggc agtaaagaaa
      901 aatacacttt ttccagtttc tacgtagcct gctaattctt ctaaactatt tgggataatc
      961 attctttttc ctctactttc tgtttatgta tttttcaatt aaattataca cctttctttc
     1021 gaactaacaa acattgatcc tctttttata aaaaaaatct gacaaaaggt tgcttttgcc
     1081 agattttata gtttcactat cgcgttattt cccgtatacg atcgtgttca ctgttgattt
     1141 atctaaacct ttcaatgttt gaatcaacat ttctcgtgca gcttcaaaat cgcgaatact
     1201 gaacatcgtt tgatgcgtat ggatataacg tcccactacg ccaatcactg tacttgggat
     1261 tccttcattt tgtgtatgag cagcacctgc atctgtaccg ccttttgaaa caaagtattg
     1321 ataagggatg ttgtttgttt cagcgatatc tagcaagtac tcacgcaaac gaggaagcat
     1381 gatcaaacct ggatcttgga tacgcatcaa tgtaccttct cctaaatgtc cgaatgtgcc
     1441 atttttcgtc actgtgtcat ctgctgcaga gcagtctact gcaaagaaca gatctggctt
     1501 gaatttattg acagaagctt tagaaccacg caagcctact tcttcttgga cattcgcgcc
     1561 tgcaatcaat gtatgaccta atgtttcatt ttgtaacgct tctaaagctt ctaataccat
     1621 tgtacagcca tagcggttgt cccacgattt gctgatgata ttcttcccgt tggctgtttt
     1681 gatcgtttca gcgtaaggaa cgattgtgtc tcctggaaga acaccgtaac tcattgcctc
     1741 ttctcttgat tcaaacccag catcgaacaa aatatctgtt acttctactg atttttgtcc
     1801 gcttgttcct cgtaataaat gtgggggaac ggaagaagaa atacatggat aattcttacc
     1861 agtagaggtt ttaagtgtaa aacgttgagc agaaacaacg taagggttcc atccgcctag
     1921 aggtacgact ttaaaaagcc cgttttcttg gatttgtgtc agcatgaatc cgacttcatc
     1981 catatgtgct gcgaccatta cacgaggtgc atccgcttct tcatggtggc gcagcccaaa
     2041 aatgccgccc aaaccatcta actgtaactc atctaccagc ggttccatat gactttccat
     2101 ataggcgcga acgtcttgtt caaaaccgct tgttccttgt aattctgtta actctttgat
     2161 tcgttgaaat gttttttctt ccatatgcta ttcgtctaaa tttcagacta aactcctctc
     2221 agacttatat tgatctgcgg ataaaatatg attttttctg cagaccgatc gtatttatta
     2281 tagcttattt tttctggtag cggttattta tttcaaagaa aatatgagag tgtggtaaaa
     2341 taaacacaaa gagtagcaag tgagaagaag gaggtatatc cacatgtatg aagaaagcga
     2401 attaagctat tttaaaggcg ggttagccgt tgggatcggt ctaggattat tgagtggggt
     2461 agcttcaacg atttggtatc atcgcaaaaa agtgttatca gcagaccaag tgcttgaaaa
     2521 tatcaaaaca tcttttttaa aagaaggcgc aatcgaaggt gcttggatct cttttgaaaa
     2581 agaacctatt cggaaattcg ccgttcggtc gagtgcttat cggggtgggc tttcccggat
     2641 agaagatggc gaaactgttt attatgaatt tttagcagat gctcatacag gaaccgtatt
     2701 agacatttca agaaaaaagg gcgcaataga ttaatacgtc aaaaaaatga aataacgaat
     2761 acgcacccac tatctaaaag agaggtatcc tatccttctt ttatagtggg tgatttttat
     2821 aaaaataggg ctatttcctc ttggaatagc tagtatttgt agttattata cggtattctt
     2881 gaaataagat tagttttatt atataaggag agtgacaaat ggaatcacgt gaaaaaattg
     2941 ccgtgctcat tccttgttat aacgaagagg cgacaatttc aactgttatt gaagatttta
     3001 aaagggaatt accagaagca gatatttatg tctatgataa taattcgacc gatcgaacat
     3061 acgaattggc cgttcaaggc ggagcaatcg taaagaaaga accacgtcaa ggaaaaggga
     3121 atgtaatccg tcaaatgttt tttgatattg atgccgatta ttatttgatg gtagatggtg
     3181 atgatactta tcctgcagaa gctgcacatg gtttgcttga gaaattacgt tctggcgaag
     3241 cagatatggt cattggcgat cgtctatcaa atggaaccta ttttgatgaa aataagcgtc
     3301 ctttccatga ttttgggaat aatctggtta aaaatacaat tacttactta tataaaacaa
     3361 aaattcgcga tgtcatgacc ggctatcgag gattcaatcg aatttttgtc aaaagcttcc
     3421 ctattatgag ttctggtttt caaatcgaaa cagaattgac gattcatgct ttagataaaa
     3481 aattcaagtt cgttgagatt ccaatcgatt atcgcgatcg tcctgaaggt agcgaatcaa
     3541 aattgaatac attctctgac ggattcaaag tgatcatgat gatcatcaaa atgtgcaaag
     3601 attataaacc tttgcttttc tttgggatct ggaccatctt cttcttttta tttggattgg
     3661 tcatggggat tcctgtgatt cgtgaatata tgctgacaag ctttattaca cgcatccctt
     3721 ctgcgatttt aagtactgga ttgatgactt tagcccttct ttcattagta acagggttga
     3781 ttttggatac ggtcgtcaca aatgccaaga aagaatatga attgaattta taccatgtct
     3841 accacgaata tcaaaataaa gaaatgaagt aggaggcctt ttttatgcat aagaaaaagg
     3901 agaatctatt tcctactttt tttgcaactt tatttgcgtc aggttttgct ttttttactt
     3961 cttatggtct aggaactttg aatccaacag taatcattgg tttagccatg gttttgctac
     4021 ttgcttttaa gcaatatgga atagagttgt tttcaataaa aaacaaagtt gcagctattg
     4081 cccgattgtt tttttctgca gcttttgctc ttacactttc tattggcaga cagatacaat
     4141 ttcagtatgt ctcagcaaac tatacaaaga caacgattgc tgatggaagt attttttact
     4201 ttcttgttta tttctccatt tttgtgtatt cttttttatt tttacaatcg atttatttat
     4261 caatgccaaa actggaaaca ttttttactg ctcaaaacac acaggctttc tcttcaagaa
     4321 aatatttttt attctttact gtctttttag ttctgtgttg gcttccttat ctgttggcat
     4381 tgtatcctgg agtggttttg cctgattcgt taagttcggt cgcccaatct atgggtgatg
     4441 ttcctataag caatcatcat ccagtcttat tcaccttact ggtcaagcta tttgttcatg
     4501 atttaccaac tgatacgatc aatcacggtg tatttttatt ttcgattttt caaacattga
     4561 taacagcttc agcgataagt tattctcttt gctggctgtt aaaaagaaac atccattttt
     4621 ttccggtact cattagttta tgctattttg catttgcacc ggtttttcca atctacgcct
     4681 tgaacatgca aaaagatact ttgttctcta tcgcttgctt tttattttcc ttagcggttt
     4741 ttgactttat ttttcaacga gaaaagccaa cgattctcaa tcagatttac gtaatcatcc
     4801 ttgctttact gaccacctat ctaagaaata atgggattta tgtagtaacc ggcacaggtt
     4861 tgatttgcgg cttgattatt ttgaaacaaa aaaggtatca agcattcttt caagtggtgc
     4921 tgggttatac agtgattgct ggactactca tccacccgtt gatggctcgt tatgctattc
     4981 ctacagaaac tgctgaacaa ttaggtgttc ctttgcagca aatttctaga actattgtta
     5041 tggatggtga cttgtctaag aaagataaag cattcatgaa tcagctactc ccgataaaag
     5101 attatgaagc gtattctcct agtcttgcag actcaatcaa gtggcataaa aattttaaca
     5161 atgagttttt gaatacacat aaaaaagaat ttctatcact atgggcaaga aatttaccaa
     5221 gtaatataaa aatctataca gatgcttata ttttagaaac gttcgggttc tgggtacctg
     5281 gtacaaaaaa tagttacggc tttttagata ctcgtgtaaa cacgaataat tatggtatga
     5341 agcaagtaga tttgattgaa catcttactg gattgtcagg aatgaaaaaa atcatcgatg
     5401 ctcgtgattt ctttggttcc ggcacattac tgtggttagc tatcggcagt gcacttttag
     5461 caatctttaa gaaaaaatat cggtatttac tcctttttct tcccggtatg cttgtttttg
     5521 ctactattat gattgctact cctgtggctt ttagtttacg gtatgtgttt gttttagcat
     5581 taggtctacc tgtttatatc ctctctcctt ttttactaga taaaagagaa aaaatgaaaa
     5641 cagatatagg agatactcat gaaactattt aaaaagatat tttttgcttt tgttctcttg
     5701 atcgctagct attgcgtact actgacaatt acttttttca ttccgcagca ctctattgaa
     5761 tcgaatgcag aaaaatctct acagatggtt gaaaaagaag gaatgtatcc ttctatcaat
     5821 caaggaaata tgctaggcac tcggttagat aattttactg atcatttgat gatacgaaaa
     5881 acaaaagcag atcctgaatt gaactccttg gagaacgcga tgtctatggc ggattatccc
     5941 cgttattggc atggttatca gcttttttta cgacctttac tgctagggat gagtttgggg
     6001 agtattcgaa tgatttacgc agccattctt tttctgctga ttggattgac gagctacttt
     6061 ttaaccaagt gtagcgacat ctatttaacg acagccttgt tgatttcgct tgctatagga
     6121 aatgctgcaa cctttttctt cagtatgcaa ttttcaaata tttggatttt gactttattg
     6181 tctgtattac tatttttaac aaaaccacaa ctctccaaca ccaaccaaca attattcttg
     6241 tatttctttg tcattggttc tttagctaac ttttttgact tgttgacaac accggtcatc
     6301 tcttggggga tacctgtgat cattatttat tatgtaacaa ataagtattt aatggataaa
     6361 cgcagttcat tgagcaaaca agtaggcagt tttgttttta ccggtatctt ttggggatta
     6421 ggctatggtt tgacatgggt ggctaaatgg atcctatcat cgatcatatt gaacaaaaac
     6481 attgtaaaag atgcaatcaa ccaaatcctc tttcgaacgg aaggaaacga caaatacccg
     6541 ttgcatcgat tagagatgct gaaaagcaat attagattga tgtatccaaa agtagcaata
     6601 ttattgctat tgattacttg tcttgttttt ctctattttg cttatcgtaa acgaaatagc
     6661 tggcgtccgt tccaacttct agttttgttt gttttagctg ctgtaacgcc gtatatttgg
     6721 tacaatattt tagctaatca ctcacagatt cattattggt ttacgtatcg tacgcaaatc
     6781 atcacaagtt ttgctatact tagtgctttc gcctttttga tccctcctga gaaatcaaaa
     6841 gacgaactaa attactttta agaataccga tgtactaatt catttttaaa cataaaaact
     6901 ccagctgtca gagatagccg gagt
//
LOCUS       DACEZN010000138         6735 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEZN010000138 DACEZN010000000
VERSION     DACEZN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.138"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..2220
                     /gene="polA"
                     /locus_tag="IXH60_002286"
     CDS             <1..2220
                     /gene="polA"
                     /locus_tag="IXH60_002286"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1991177.1"
                     /translation="TQLASKEVKVDITVKGVSDIESYTPEHVAEKYDGLTPKQIIDMK
                     GLAGDASDNIPGVTKIGEKTAIKLLKQYGSVEGIYEHIDEMKQSKMKENLINDKEQAF
                     LSKKLATINTSAPVDVSIDSLKYEGKNLDKLVPFYKEMDFNQFLSKLNIVEEEVKMDD
                     ILFEVVHEFKEEMFTTDMALYVEMMGDNYHTEEIVGVAWGTEKKIYVTNDLSVFENRA
                     FHSWITDPTRLKKVYDAKRTYVALNRYTGKTKGIDFDVLLGAYLLDTNEKSTDIEGIA
                     AHYGYNDIQSDEAVYGKGAKKGLPEEEELFFAHLARKVAAINALTPKLSQELADKNQE
                     DLFRKMELPLSIILAEMEISGIKVDATRLQEMKGEFSARLREIEQKIYEEAGEEFNLN
                     SPKQLGVILFEKMGLPVIKKTKTGYSTAVDVLEQLREQAPIVEDILTYRQIAKIQSTY
                     VEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVDPNLQNIPIRLDEGRKIRQAFVPREKD
                     WLIYSSDYSQIELRVLAHISNDEHLKAAFLEGQDIHASTAMRVFGIEKAEDVTPNMRR
                     QAKAVNFGIVYGISDYGLSQNLGISRKAAQQYIDTYFEKYPGVKEYMERIVREAKDQG
                     YVETLYHRRRYLPDINSRNYNIRSFAERTAINTPIQGSAADILKIAMIELDKRLKETG
                     LQATMLLQVHDELVFEVPEKELESLDKLVKEVMEQAVSLHVPLITDSSWGKTWYEAK"
     gene            2265..3101
                     /gene="mutM"
                     /locus_tag="IXH60_002287"
     CDS             2265..3101
                     /gene="mutM"
                     /locus_tag="IXH60_002287"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1991178.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            3065..3694
                     /locus_tag="IXH60_002288"
     CDS             3065..3694
                     /locus_tag="IXH60_002288"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1991179.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            3746..4078
                     /locus_tag="IXH60_002289"
     CDS             3746..4078
                     /locus_tag="IXH60_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1991180.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            4390..4881
                     /gene="nrdR"
                     /locus_tag="IXH60_002290"
     CDS             4390..4881
                     /gene="nrdR"
                     /locus_tag="IXH60_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1991181.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            4904..6277
                     /locus_tag="IXH60_002291"
     CDS             4904..6277
                     /locus_tag="IXH60_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1991182.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            6277..>6735
                     /locus_tag="IXH60_002292"
     CDS             6277..>6735
                     /locus_tag="IXH60_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1991183.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTAD"
BASE COUNT         2402 a         1197 c         1480 g         1656 t
ORIGIN      
        1 actcaattag ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc
       61 gaaagctata caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt
      121 attgacatga agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc
      181 ggtgaaaaaa cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa
      241 catatcgatg aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag
      301 gctttcttat cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc
      361 gactcattga agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg
      421 gatttcaatc agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat
      481 attttgtttg aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg
      541 tacgtggaaa tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg
      601 acagaaaaga aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat
      661 tcttggatca ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt
      721 gctttaaacc gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca
      781 taccttctcg atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga
      841 tacaacgata tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca
      901 gaagaagagg aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg
      961 acaccaaaat tgagccaaga actggcagat aaaaaccaag aagatctttt ccggaaaatg
     1021 gaactgccat tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg
     1081 actcgcttac aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa
     1141 atctacgaag aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc
     1201 ttatttgaaa aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc
     1261 gtagatgtac tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat
     1321 cgtcaaattg caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca
     1381 gatggaaaag tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct
     1441 gttgatccta atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag
     1501 gcatttgttc ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag
     1561 ttacgcgtac ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt
     1621 caagatatcc atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc
     1681 acaccaaata tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct
     1741 gattacggac tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat
     1801 acgtactttg aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca
     1861 aaagaccaag gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat
     1921 tcaagaaatt acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa
     1981 ggaagtgctg ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa
     2041 acaggtcttc aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg
     2101 gaaaaagaac ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct
     2161 cttcatgtac cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa
     2221 acattgttta agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag
     2281 aagtagaaac cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag
     2341 ttcaggtctt gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc
     2401 ttgttggcga aaccatccaa tccattggac gtagagggaa gtttttaatt tttcatctcg
     2461 atcattgtga actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa
     2521 atacaccaat cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc
     2581 gctacaacga tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa
     2641 cttaccgagg aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag
     2701 atttcgcaaa tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac
     2761 gtctagtgac tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc
     2821 atccagaaca accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga
     2881 tcattgatgt tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga
     2941 atgcattagg agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca
     3001 aaccatgtat acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc
     3061 actattgtcc gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac
     3121 gggtggaata gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc
     3181 tttgatcgat ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag
     3241 gaaaattgta tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg
     3301 gaaacttggg atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt
     3361 agatccgttt atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc
     3421 tttagtaatt gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc
     3481 agtagctgtc gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca
     3541 actgacgcta gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa
     3601 gcagcgtgcg gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat
     3661 cgaaaattgg ctttttgcta atcgctttct ttgagtatct gtgtcaattt tgctatcctg
     3721 agtatataga aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt
     3781 aggtaacgca aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg
     3841 tcgtacgcaa tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga
     3901 aatagaagac cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa
     3961 aaatccgaca cctgatcgta gtaaaacacc gctcccagaa agtgaccaca tttccgatga
     4021 gcaaaaaatc gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa
     4081 tagtaaaaaa tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaagttt
     4141 tttgtgctag atgactaaat cagcttttgt gtaagttatt tctcagtaat caaggtgatc
     4201 agtattggaa aattgaaatt tagtaaggac aggggacaca attacgcatg aaagtagaaa
     4261 aaattgttct tatatccgtg ttttttgggt aggacagtcg cgtttgtgat ttgtttcgtg
     4321 ttataataca actagttgta aagaaagaca catcctttat ataaaatgcg atgcaagagg
     4381 tgagacaaaa tgagatgtcc cagatgtcaa cataataatt cacgagtaat cgacagccgt
     4441 caagcagatg atggacgtgc gatccgtcgc cgtcgcgaat gtgagaattg ttcgttccga
     4501 ttcactacat ttgaacgaat tgaagctgct ccgttattag taatcaagaa aaacggggaa
     4561 cgcgaagagt tcaatcgcga taaaatttta agaggactca tccgttcagc agaaaaaaga
     4621 cctgtagcta tggaacaaat ggaacaaatc gtcaaccatg tcgaaaaccg cgtacgcagt
     4681 ctcggagaaa acgaagtatc gacgaatcta attggtgaat atgtgatgga agatttagtt
     4741 gatttagacg aaatcgctta tattcgtttt gcaagtgttt atcgccagtt caaagatatg
     4801 tctgttttct taaaagagtt gcaagatatc gtggataaag caaaagaaaa taataaagag
     4861 aataataaag aaaacaatta agaaacaaaa aggaggcatt tccttggaaa atgcttggaa
     4921 agagcttcaa ccaaaaaata tttaccaagg aagaaaagcg gctccgctga gagaagacag
     4981 caagaacgcc ttgctgtatc tttatcagcc actaatcggc ggagaagcct tgagtatcta
     5041 tttcaccctg ttatcagaaa tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc
     5101 agatctgctt agtagtctag gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct
     5161 agaaggaatc ggcttactgg atgtttattt taaagaagat agtgatctgg gacctcgttt
     5221 tatttatgag ctgttagaac cactcattcc taaagcattt ttcaaagact ctgttctggc
     5281 ttttcttctt ctggaaaaag ttggtgaacg acgattccag aagcttaccc aattctttga
     5341 acctaaaagg catacagcag agggatatca aaaaatcaca aaaaaatttt tagatgttta
     5401 tcgcttcaat gaagcagtgt acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc
     5461 ttttgaagaa tctgaatcag tagcattact tgaagcagat tcgacattcg attggcagtt
     5521 tttgaatgat tggctggcaa aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca
     5581 agtcgacatg tatcatcagc tttacggttt cgatgaattg acacttggcg aactgatcag
     5641 tcaatcttat gactttagtg aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt
     5701 ggcaatgcag agacgtccgg tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag
     5761 taatgaaccc tcgtcaaacg aacatttgac aaacgcagcg cttcagctga taaaagaagc
     5821 acagagcatt ccaccgatga aatatctgga agcaatcaag tcagaaaaag gcggatatgt
     5881 ttctaaacaa gaaaactggt tactgcaaga tcttgtttct caatcaggtc tttccagtag
     5941 cgtgatcaat gttttgctga attatgtact agtgatcaag aataatgcta gcttaaatgc
     6001 cagttatgta aatacagtgg caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga
     6061 agcgatccag cacattcggc aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca
     6121 aacaaattat caaactggca agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa
     6181 ccagccaaaa gatgaaaaac agatcagcag cgagaaaaaa gcagagattg accgccgttt
     6241 taaagaatac ctggcaaaaa aggagggtga tatctagtgg aagacgtagg caaagaattg
     6301 aagaggatca tcagcaaacg caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc
     6361 ttaaatgatg cggatgttca agcttttcta agcgcacacc aagaagaatt gacacaagcg
     6421 gatattgaaa aaagttacgc taaattgtat gaattcgtgc aagaaaagcg taaatatcaa
     6481 aaaaacgatc caacgatgat cgctcccggc tatgagccga agctgacgtt gaattttcat
     6541 tatgtggatg tcacttatgt accaacggaa gcattgttag cacggcaaag acaagaagaa
     6601 atcagaagcc gagtaaaagc aatggatatg ccaaaagata ttcaatcggc ttcttttgaa
     6661 aactttgaac gtacagatgg acgaggatat gcgagcttag aagccctaga ttttgtagag
     6721 aaatatactg ctgat
//
LOCUS       DACEZN010000139         6670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEZN010000139 DACEZN010000000
VERSION     DACEZN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.139"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1006)
                     /locus_tag="IXH60_002293"
     CDS             complement(<1..1006)
                     /locus_tag="IXH60_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1991184.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     A"
     gene            1228..1902
                     /locus_tag="IXH60_002294"
     CDS             1228..1902
                     /locus_tag="IXH60_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1991185.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            1907..3247
                     /locus_tag="IXH60_002295"
     CDS             1907..3247
                     /locus_tag="IXH60_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1991186.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(3396..4382)
                     /locus_tag="IXH60_002296"
     CDS             complement(3396..4382)
                     /locus_tag="IXH60_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1991187.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            5106..6137
                     /locus_tag="IXH60_002297"
     CDS             5106..6137
                     /locus_tag="IXH60_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1991188.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            6246..>6670
                     /locus_tag="IXH60_002298"
     CDS             6246..>6670
                     /locus_tag="IXH60_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1991189.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         2173 a         1211 c         1148 g         2138 t
ORIGIN      
        1 ttgcgacaat gatcaaaatc ccactatata gcaaccattg tgaccaaaaa acagcaaaag
       61 gcatcgcgct tggtaaaatc agactagtca aataagcaaa caaaagagca agcagaacgc
      121 ctaccacccc aacaataaag gactgtgcta ctaaagattt agcgatgaag aaatttttga
      181 tcccttgtgc tttcattact ccaaaaatag cagtcttctg aagtgtcatc acgtacatga
      241 aaataccgac cacggcagca accactaaga acaaaaagta tatcatcgca tttaaggtca
      301 tattttgggc agaatagcca ggaatacttt caatgaatgt cccaatagtt aataactgta
      361 gacccttcga tgctttttct ttcgaaattt tagctgaatc ctttattacg atcccattga
      421 tcggctgttc ttcttcagaa gcaaacggct gttgtccata cttcaaagca gtccacgtat
      481 ctagggacgc atagatgacg ggagaaactg tgtaataagt ttcaggaaag ataccgacaa
      541 tcgtcaaagg atcgtcgtaa ctgccaattt tgatcgtatc tccaatttta tagcctgaat
      601 cagccagatt ttgagagatg atgatttcat tctttgtttt aaattcatgc cctttcgtga
      661 gttttggtaa taaaaatgct tctttcgttg tgccgaaaac tgtaacattt tctttgtttt
      721 ttcctttgat tgctccgctg tacaatccga ttggtgcttt ctctcctcct tcaatgtatt
      781 tcagatctcc tcttgttaat tgtgaagctg caaatacttg atttgcatcc tccgacaaaa
      841 caatctcttg tgcgtcccaa tcatcgattg cttttttaaa ctcttctgaa agtccattcg
      901 ctagtcctga caacatgaat acgacatacg caatcaagaa cataataccg acgatcaagc
      961 catatcgtaa tttagaatag cgaatttctt tgatagctag aaacatttgt ttttcctccc
     1021 tatgatagat tgatagtgac aaaatgtcac ttgttgaata gtgtaactct aaagtgacaa
     1081 tttgtcactt gtgttgtttt tttcttatca ataaattgtc taagacatct ttcgataaaa
     1141 ccttaacatg agtcataaaa aatttattta agccttattt atgctttcca tgataaacta
     1201 aagtgaccaa atcaatgagg tgggaaaatg attaaactat tagtagtaga agacgataaa
     1261 gtattatcca ataatataaa agaaatactc gctgcaatcg gcgagataac acaagtttat
     1321 gatggtagtg aaggactgtt cgagttggaa agtggagttt atgatcttgc aatcttggat
     1381 ttgatgatgc cggaaatgaa tggatatgaa gtgttagcaa aaatacggaa agaaaaaata
     1441 cagactccag tattgatttt gactgcaaaa gatagtttgg aagataagat tgaaggattt
     1501 gaacgaggtg cagatgatta tctaacaaaa cctttctatc gagaagaact gatcatgcgc
     1561 gtgaaagcat tattgaaacg ctcgctaggt ctctttgatg aaaatgttct gaagtataag
     1621 gatatcagtt gtgatttaaa aagcaatgaa gtcttgtata aagaacaagt tttgcccata
     1681 caaggaaaag agtttgaatt gctcgtttat tttttacaaa ataagggaat cattttgaca
     1741 aaagagcaga tatttgaccg gatctgggga tttgattctg atacgacgat tacagttatt
     1801 gaagtttata tgagcaactt acgaaaacat ctgcgaagta ctggattgga taaagaaatc
     1861 aaaaccttac gtaatgtcgg ttatatactg caaagggtgt gatgcaatga atggagatat
     1921 tacacgaaag caacgtagtc gcttttttgc gacaaatgta gcagcatttg cttttatctt
     1981 ttttcttttg ggattcatta ctttacagat attaaaccaa tctgcataca gagaaacaga
     2041 tctgtcatta gagaaaataa attttgattc tcgaacagta caattagaga tacaaagata
     2101 tcagcaaggc gatccctttt taaacccgac tcaaacggaa caaaacagaa caaacatgga
     2161 acagccatta atggaaccat caaatgaccg ttttaacact caagtgatct tgtggtctaa
     2221 aaatggtgag atattgaata aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt
     2281 aaaaatggat acgaaaaatc tgaataccat ccaaaatatt gaattaaatg aagacagtgg
     2341 ctcatctgcc ctctctttcc gttcgattac gaaaaaagcg gagacgaatc aagatggcgt
     2401 tgcctacatc caaattttag aaaatacaaa tcaaatcaag aattcattag cgacttttcg
     2461 cacgattatg attttgtgta tgattgtttt ttggattctc tctattggta tcagttatta
     2521 cttgtctagt ctaagcatgc gccctattat tctttcttgg agaagacaaa aagaatttgt
     2581 tgagaatgct tctcatgaat tgcgcacacc gttaacgatt attcaaaata gtttggaaca
     2641 tctttttacc aagcctaatc atactattat tgaagaatcc gaaagcatcg ctcaagcatt
     2701 gtcagagacg aggcgcctca ctggattgac ttcggatttg ctgacaatcg ctcggaatga
     2761 ttctgatcag cagctgcttt caaagcaaat gatcaataca caagaatata tcgaacaact
     2821 tgttaaacca tttcaagaga tggccatcat agatggaaaa gagtttattc tccataacga
     2881 tgcaacatta gaagtaccaa ttgatgaaaa aaaaatccat caagttttgg ttattttgct
     2941 ggataatgca ttaaaatata cgaatccaga agataagatc attttggatt catctgttaa
     3001 ccataaagaa tggctgattc acgtaaagaa tactggccct tctatcagtg aagaagatca
     3061 agcaaggatt tttgatcgat tttatcggga agatcaatct cgggcgaaag agactggcgg
     3121 atacgggctt ggcttggcaa ttgcaaaaca ggtaatcgaa cagcatcatg gacagatttc
     3181 tgtatccaat tatcttccac aaggagtcga ttttaaagtc caattgccgt tgaagcctaa
     3241 aaaataatgc ttatccctaa aaaagggctg tgacaaaaat cttgtcacag cccttttttg
     3301 ttgcgaataa acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttctttaag
     3361 ctgaccactt ctgtcacaac ctctttagct tacgcttacg attttttatt gcggtcttca
     3421 atgtgtacat caccgcttgt gtcaatatcc aattcttctc ggctaacatc ttctgtcact
     3481 ttttcaacat cttcatgttc ttctttatga atcccaactt cttcacgaat aactgtatgt
     3541 ttgcttacat ctacttgctc ttctttgatt gggatagtaa tcgtgtcatc ttccaatttt
     3601 tcgtctgttc cctgtgaaga ttgatctgtt actggtttgc gttcgatgac gatttcttca
     3661 cgtttgaccg gtacttctac cgtttcagta tcgttgacca catgtttgtg gatgtcgact
     3721 tctccagttg tcacatcatg tgtatttaca tctaattttt cttcttttag ttgaattgta
     3781 tcatcatcta gatttggttt gcgatcgatg tcttcagtct tgcttgtacc cattgtatca
     3841 gtctcgccag gtacgtccat cggatcagtt cctggtgtca tattcaatgg atccgttgta
     3901 ttttgaaagc ctgtcgtgcc acctacgtcc attggatctg ttccagtgcc agtttctaat
     3961 ccagtcccca ttgtgcttgc tgggctaaca gtatcttgtg tatccattgt atcttctgtt
     4021 gtttctggct gacgatagcc ttttactgtg atgacaaatt ttccatcaga aatatctcct
     4081 ctatattgat ataaaggatc attttctggt gtagccgtct cagaatcata ggtatcaaaa
     4141 gaaaaagtat cttttatttt ttcccataaa gaacggtctt cttcaccatc catccgattt
     4201 tcatctgttt caacatctat accagacaat ccgtcaaatc ctagtctaga tgctgcttca
     4261 tcagtagtat aaataacgat atcatctcgt tcataccctt cgttacgcaa ccgttcaatt
     4321 actgataatg cttcttccct cgtactatag cttccgatga ccgttaaatt tttctcttcc
     4381 atgtaaattc ctcctagcaa atagatttat gattggtata ttgttactta taaatttatt
     4441 ctataggaga cgattgtttt ctacaaatat ttagattgga atttgattaa aacaatttaa
     4501 ttgaaatttg aaaacattta tgttaatgaa agtcatattc taataaaatg tcatttgttt
     4561 ctatatgaat ttttatgaaa aatttcatat aatttccact cgttgtgatg gtttaattta
     4621 tccaatccat caataagcaa acaacttata taaaaaagga ttaattctca atataattgc
     4681 tcctaccata gaataaatct tcatgtaaag taatgaagta ccaactattt ctaatacttc
     4741 tttaagaatt atcccaacaa tatcgttaga caaaccatta ttgaataaat ggagagccaa
     4801 tcgattctga aaaaatgttt tctaattttt cggaaataaa agaaagagcc tcctgccatt
     4861 agaacgaacc aaatccattt cattgctcat gaagtaacca catagctaaa aagaggataa
     4921 atcaattaaa agatcaaaag aatgactgca ctaataaaat aactaaaaat gtatcttctt
     4981 acttcatatc ttttagctag tacttagtta aaaatttgct tcctaagatc aagacaaact
     5041 gtctatcttc atttgatgaa atgcggtaaa atatagaaaa tgaacactat tggagggaat
     5101 ttgtaatgga cagcaaaact agaaaacaac tgcaagaaaa gttaaagcaa cgtacacact
     5161 tgctcccttc cgtctttcaa cagtttttag aagaaaatcg ttcttccctt tcattaggca
     5221 gtcgctatga atacgcaaaa gattttcatc tgtttactga ttatttgaga aaagtatttt
     5281 ctgaagaagt ggatgatcag ctgattatcc atttagaaaa acaagattat cagaatttct
     5341 tagctaaaat ctatcgacat actcgttcgt tccaaactac agcgaaccaa gaaacggagc
     5401 aattgtttga aaatagctat tacgggattt caaggaagca atattcatta aatcattttt
     5461 tacgattttt gtatcaaaaa ggactgattg aagaagagat ttctcttttg ggtacaagtt
     5521 taccggaaac aaccaaagca gctccgcgat atatcgatgc agaacttttc aaagattttg
     5581 agcatttgtt tatcccgatt gaaggctttc aaacaagcag agaagctagc tttgaagaaa
     5641 agaatagacc gaggaacctt gcaattactg ccattcttct gtattgcgga ttaaagatcc
     5701 atgaagtagt agaacttaaa cgtaaagatg tatctttgac caagcagatt cttaatctga
     5761 atagaaaaga aaacagactg aataaagtac caataccaaa agaagcgatg ccatttctgg
     5821 agaactattt taagttaaca gcacaaaata cggacgaaaa ttcttttgtt tttcgttcct
     5881 cacatgatca gcaaatttct cctcggacta ttcgccaaat tttgagcaaa attacagtat
     5941 ataaaaacgt atcgccagaa ctatgcagaa aaacatatcg ttattatgca gaactatatt
     6001 tagatgatgc tgacgcggta aattatttat gtggtaatcg atacctctca tcccattctt
     6061 ttcaagatat ctgggaaaag atggttgatt tttcttatca cgaattagcg gcattcgttc
     6121 gggtttcttc catataaaac aaatgtatga tctttttttc acaataaact atgttattat
     6181 ataaaggatt atgttacgat tatcttgaat ttgtatatta cttaacaaaa gataatggag
     6241 tgtgaatgat ggagaccata acgtattccc ctttgaactt atttacgaat tttcaagaag
     6301 ctgcagctaa cttttccgag accccgatta tctttgatga gccactttca gctttcccag
     6361 aattaggact tgaaacaact tacaaaaaat gctgcgaaag tgtgttgaaa cgggcttatc
     6421 aattggctca tcttggagtg aaatccggag acaaagttat tatttataag agttccgctt
     6481 ttgacacata ccttctagct gtttctgtgt cttaccttgg tgcagtgcct gtcatgactt
     6541 cttatcatct accaacaaca acaatggaag tatttatcga tcgtttagaa gaccctttca
     6601 ttttatttga tgatgagaca aaaaagcgag tgagagaaat ttcaaatgga acaaaatcaa
     6661 aacagattcc
//
LOCUS       DACEZN010000140         6616 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEZN010000140 DACEZN010000000
VERSION     DACEZN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.140"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..646
                     /locus_tag="IXH60_002299"
     CDS             <1..646
                     /locus_tag="IXH60_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1991190.1"
                     /translation="LTVEKDIYEDFLVSDHITTTAYLRISLPNLLAKEDYKKVLYLDS
                     DVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYFNSGVMVIDIDQWN
                     KKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQTSLIFERHPAPNE
                     KYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHSTLTKVGEINE"
     gene            639..1541
                     /locus_tag="IXH60_002300"
     CDS             639..1541
                     /locus_tag="IXH60_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1991191.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1574..1924
                     /locus_tag="IXH60_002301"
     CDS             1574..1924
                     /locus_tag="IXH60_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1991192.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            1958..2896
                     /locus_tag="IXH60_002302"
     CDS             1958..2896
                     /locus_tag="IXH60_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1991193.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            2974..3867
                     /locus_tag="IXH60_002303"
     CDS             2974..3867
                     /locus_tag="IXH60_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1991194.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            3905..4468
                     /gene="amaP"
                     /locus_tag="IXH60_002304"
     CDS             3905..4468
                     /gene="amaP"
                     /locus_tag="IXH60_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1991195.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4481..4669
                     /locus_tag="IXH60_002305"
     CDS             4481..4669
                     /locus_tag="IXH60_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1991196.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            4682..5584
                     /locus_tag="IXH60_002306"
     CDS             4682..5584
                     /locus_tag="IXH60_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1991197.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5616..5858
                     /locus_tag="IXH60_002307"
     CDS             5616..5858
                     /locus_tag="IXH60_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1991198.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6114..6485)
                     /locus_tag="IXH60_002308"
     CDS             complement(6114..6485)
                     /locus_tag="IXH60_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991199.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2397 a         1142 c         1314 g         1763 t
ORIGIN      
        1 cctgacagtg gaaaaagata tctacgaaga cttcctagtc agtgaccata tcacaactac
       61 cgcttactta agaatttctt taccaaactt attagctaaa gaggattata agaaagtcct
      121 ttacttagat tcagatgtgt tagtactaga cgatatcgta aaactatatg atgagccgtt
      181 aaatggaaaa acaatcggcg ctattatcga tccaggccaa gtgaaagctt tggaaagatt
      241 aggaatcgat tccgatgact tgtacttcaa ttcaggagtg atggtgattg acattgatca
      301 atggaataaa aaagaaatta cagaaaaaac tatccattac ctcagtgaaa atggcgatcg
      361 aatcatctat cacgaccaag atgctttgaa tgctgtactt tatgaagatt gggaacaact
      421 tcatccaaaa tggaacatgc agacttcgct gatttttgaa cgtcatccag caccaaatga
      481 aaaatatgaa cgtcagtata aagaaggaaa tgaaaaacct tcgatcgttc actttacagg
      541 acatgataaa ccttggaata cgttgaaaga tcatccgtat accaatttat acttaaaaaa
      601 attagcacac agtactctaa cgaaagtagg cgagatcaat gaataaaaaa gaaatagcag
      661 tagtcgcaag ttgtaacaca aaattcgtac cacatttagc tgcattattt gtctcagtat
      721 tagataattg caacccgtct aaatttgttc ggttctatgt gattgacgat gatatcgatt
      781 ttgaaagtaa acagctttta cgcttctcag tgaaaaacgc acgaatgaac tcagatgtgg
      841 aattcttgaa aatcaataaa gaattcttta caaatgtggt aatcagcgat cgtattccag
      901 aaacagctta ttatcgtatt gcgattccgg aattgttccg cggcacagaa gttgaacgta
      961 tcttatatat ggattgcgac atgatcgctt tacaagatat ctctaaatta tggcgtcttg
     1021 acttcggtga ttcaatcgta gcagctgttg aagacgctgg gttccatcaa cgactggaaa
     1081 aaatgaaaat ttcagctaaa tcaatgcgtt atttcaactc aggtttgatg ctgatcaatg
     1141 tgaaaaaatg gttagatgaa aatattacgc aaaaagtatt agatttcatc gaacataatc
     1201 cagaaaaatt acgattccat gaccaagatg cattaaatgc tatcttacat gaccgttggt
     1261 taccacttca tccgcgttgg aatgcacaag gctatatcat ggcaaaagca aaaaaacatc
     1321 caacagcagc aggtgaacgt gaatacgaag aaactcgcaa caatccttac attatccatt
     1381 tttcaggaca tgtgaaaccg tggagcaagg attttgaagg gccaacgaag aaatattacg
     1441 aaaaatatgc agggatgacg gctttccgtt gtgtagctaa attccctaaa tatccaaaat
     1501 atgcaaaagt tcaaccaaaa acagatgtag ttgttaaata atagaagaca acaactgaag
     1561 agggggagta ggaatggaaa ttacaatgtc taaaaatgca gtagagacgt taattgaaaa
     1621 ggttggggaa aatacaaaaa tcgcactcgc gcttatcaat gattccgatc catttctaag
     1681 ggacaaaggt gcttttgcaa aaggcagctt ttttcaaatc attccttttg tttcggaatt
     1741 tggagaatat gcgactaaaa tcgagcatcc attattggac atctatacat ctaaattaga
     1801 gcaaaattat ttcggtaaac gattgaacat ggacttcaac aaacagcttg acagcttttc
     1861 attagaaaat gaaatcgcag tactcgatta taatatcaaa ttaaagaact gcttcttcag
     1921 ctaatagttt cgagaaagtc caaaaaggag gtatacattg agatactact ttgtggatga
     1981 agaaaagaat gtaatagaaa cagatgaaaa aaaatataat tggctcgtac ttgatagtgc
     2041 aaagcccgaa gagattgagc aagcaatcga agaattcgag cttcctgatg atattttcgt
     2101 aggaaccgaa tatccggaag aagtttctcg gatggagcgc ctttatgaga cgaaactaga
     2161 gcatccattt tctttggtag tgattaatct agatcagtca gataatcgta tcgaaaaaaa
     2221 attaacgcca atatcttttg tactttccga taatctcttg attacttgta tcgatagaaa
     2281 aaccgaattt gtggatcggc tgctgacaaa gcatggaaat catttaaact cgtttgaaaa
     2341 gatcatcacg tatacattat tagatattta cacccattac gtgaaagaac taagagagat
     2401 gaaaaagcga attgacgctt tggataaagc agctcgtaaa acaacggaaa atgaagaact
     2461 gtataaacag gcagatcttg aaagagacat cgtatatatc gatcatacct taagagatca
     2521 acgggaaaca atggatcaat tatggaattc tgaaactttc atcaaacgtt tagacgatga
     2581 acagctactc tatgatgtcc atttgagaca gcgacaaaca gaaaaaatga tcgagatcta
     2641 tcgagattta ttagagagta ttggtgatct attcaacggg atgatggaca ataatctaaa
     2701 tcatatcatg aaatatctag attcagctgc attaatcatt tccgttccgg cgttgttcgc
     2761 agggatttgg ggaatgaata caggaggatt accgggtaaa agttctgctc taggctttat
     2821 actcgtagtc tttggctctt tgatagcagc agtagtttta ggttaccatt tataccggaa
     2881 agattataca aattaaagaa tctatttaaa acaggtatca aacaaagaaa tttgttttgg
     2941 caatacaatt taaaaactag gaggaataca gcaatgacag aaccaaaatt aatcgacccg
     3001 cgaaacttgt accatacgga gaaatttcct aaacaagatc aggatacacc agctttacag
     3061 cagaatatga ttcctgttcc tgattgtgga gaagaatcct atgaaggcaa taatcaacta
     3121 gaaaaccgcc gtgtactaat cactggtgga gattcaggga tcggtcgtgc agcagcgatt
     3181 gcatttgcta gagaaggggc agatatcgct cttcaattct tcccaggaga agaaaaagat
     3241 gcagaggaag tagcaaaata cgtgaaagtt gcaggacgta agattgtctt actacctttt
     3301 gatcttcgtg acgaacaagc acctaaagaa atcatagata aagccgtgag tgaacttggc
     3361 gggctagata cgatggtact aaacgcagga cagcaaattt cttgtcagtc tattgctgag
     3421 ttaccacttg agcaagtcaa agacacattt atgataaaca tcatcgctat gtttgcttta
     3481 gtgaaagaag ctgttccgca tatcccagca ggaggagcaa tcgtaacaac aacttctgtt
     3541 caagcattca atccaagcga acatttatta gattacgcag caacaaaagc ttcaatcgct
     3601 aactttacga tcggtttagc aaaacagctt gcacctaaag gaatccgtgt aaatggtgta
     3661 gcacctggtc caatctggac accattacaa ctagacgaag gacaaccttc tgattcgatc
     3721 cctgaatttg gtcagcaaag tttactagaa cgtgcaggtc agccaggaga attagcacca
     3781 gtttatgttt tcttagcatc gaataaagca agttatgtta cagcccaaat ttacggagta
     3841 actggtggag aagcgatcaa cttataagaa gttgatcgtt tcacccctat gaggaggtgc
     3901 gaaaatgaat aaaggtgtaa aaacgatcgg agtgatcgga acattacttt tgctatctgt
     3961 gcttttgttt gcgatactat taagtgcaac ctatccattg ccttttcagc tagagaagtt
     4021 tcgattcttt tccatcacta attcatacgt gcaacaatat gtattttggc ttgccgttgc
     4081 atttgctatt ttgacaatta tcacgttact tgttttcctg ttttatccaa aaacaatcgg
     4141 aaactttcta ctgaaacatg gtgatgggga gttgacgttg gataaaaaag caattgaagg
     4201 aatggttcgt tctcacttgc atgaagatga attcatccat tcaccaaaag taaatgtcaa
     4261 agcaacgaaa aacaagatcg ccgtcaatgt aaaaggggag ttgaaacgaa cttcagcact
     4321 cattggtaaa acaggaacat tgatgaaaga aatcgaaaat gaagtacaac acatattggg
     4381 aacaaaagag ccagtgaaag tatcggtgaa atacacaggc tttgaagaac cagctaaacg
     4441 aagcaatgac cgcaaacgag tagaataggg gggaccaaaa atgaaagaac tattttcgac
     4501 atacaagctc cctatcatct ttggggtact tggattgata ttagccgttt tatggatcac
     4561 cgtgggcttt ttcaagacct tattgatctt gatcatggca gcgatcggcg tcgctatcgg
     4621 tttttatctg aaacaaacga aaatcttaga tcactatttt aaatcttagg aggaaacatc
     4681 catgaataac aataacaaaa ataataacaa taaaatgaac aaccaaaaac cagtgtctgc
     4741 acatccagac ccaacgacac caaaccaatc aaatggtgtg aacgatgaaa agaacaaaac
     4801 agcaccagta tctgcacatc cagatccagt agtccctaac gttcaacaag gagtagaaga
     4861 gaagaaacat gatgcagaaa agcatgcacc tcatcatcca ggaactccag aatctaaagt
     4921 tccagaacaa catgatgcaa tcgatccttc taaacctgtg gaagagcaaa aacatccttc
     4981 tgaccatgcg aaagcacaag aaaaacttag ttctgaccac acaaacggac aaggaaaact
     5041 tggttctgat catacaaata accaaggaaa gcatgattct gaacaggaag attcaatgcg
     5101 cggtgagctg aattatgaag ataaagttgt gcaaaaaatc atcggaatcg caattgaaca
     5161 agtagacggt ttattatctg caaacggtgg tttcttctct aatgtggcag gaaaacttgt
     5221 gaatacagat aatgtaacgg ctgggatcga aacagaagtc ggtaaaaaac aagtagcagt
     5281 tgatatggat gtcattgtgg aatatggcaa agatatcgaa aaaatctttg aagaaatgca
     5341 agaagtaatc ggcaaagaag taaaaaatat gacacattta gaagtgatcg aagtcaacgc
     5401 taacgtagtg gacatcaaaa caaaagaaga atttgaaaaa gatcaagaaa cggtgcaaga
     5461 caaagtaact gacgcagcga aaaaaaccgg tgaatttgct tcgaatcaaa cagataaagt
     5521 aaaatcagca gctggaagcg gcgcacaaaa agtaaaagaa aatacagaac cacgggttca
     5581 gtaataagga agtaaaacat taggaggaac gaaacatggg atttatttgg tcacttatcg
     5641 tcggaggaat tatcggagca attgcaggag caatcacgaa taaaggttct tcgatgggga
     5701 ttattgcaaa tgtcgtagca ggtttagttg gatcagcact tggtcaagca ttattaggtt
     5761 cttggggacc aagtattgca ggaatggcaa tcgtgccatc attgattggt gccatcattt
     5821 tagtactaat cgtatcattc tttgctcgaa aactgtaaaa agacttttaa aaagaggtcg
     5881 tgaaaaagct gttcttcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt
     5941 tttcatttgg ttgggcatat tatcgaagat gcaagctttt gaacactgtt tatacggaat
     6001 aagaggctgt gacaagactt ttgtcacagc ctcttattta tgcgggcaaa ttcccaatta
     6061 gatttttaaa agtttttcct taaatgtgtc tttattgata cagagaagct tctttaataa
     6121 aacaatgcct ccctaaattt tcttgagata ggaaaggtga acatacaaaa taatacgacc
     6181 actttgaaaa cccagatgat ccacaaatgg atcgtatgat actgatgaag taaaaagcaa
     6241 tggatcagaa actcaaagag attgaaaaag acaaggaaga aataatatct cttttttact
     6301 cttgtctgtt ttagccaata atagctcgta aagtaatcta gacaaacaaa tagaagtaaa
     6361 caaagtttcg taagagcagc aatcgtatca tgttgctctg ctagacgatt gacgagcagg
     6421 aaaaatgttg gtaatggaat atagatcacg agtaatgaaa gaaaaaagta aacaataaat
     6481 ggcatactat tcctcctaat tgtgtgagtt cgccctcatt ttaaaataac gatatttgtt
     6541 tgtgaagctt tttcgtgtcg taacaaatgt tatctaatgt atttttaaat aaataatcat
     6601 tattatcaaa ataatg
//
LOCUS       DACEZN010000141         6467 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEZN010000141 DACEZN010000000
VERSION     DACEZN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.141"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(109..564)
                     /locus_tag="IXH60_002309"
     CDS             complement(109..564)
                     /locus_tag="IXH60_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991200.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IXH60_002310"
     CDS             complement(718..1926)
                     /locus_tag="IXH60_002310"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1991201.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IXH60_002311"
     CDS             complement(2038..2370)
                     /locus_tag="IXH60_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1991202.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IXH60_002312"
     CDS             2518..3393
                     /locus_tag="IXH60_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1991203.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IXH60_002313"
     CDS             complement(3475..4056)
                     /locus_tag="IXH60_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1991204.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IXH60_002314"
     CDS             complement(4062..5321)
                     /locus_tag="IXH60_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1991205.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IXH60_002315"
     CDS             complement(5323..6369)
                     /locus_tag="IXH60_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991206.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1804 a         1337 c         1102 g         2224 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaa
//
LOCUS       DACEZN010000142         6380 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEZN010000142 DACEZN010000000
VERSION     DACEZN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.142"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(303..884)
                     /locus_tag="IXH60_002316"
     CDS             complement(303..884)
                     /locus_tag="IXH60_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1991207.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(1067..1693)
                     /locus_tag="IXH60_002317"
     CDS             complement(1067..1693)
                     /locus_tag="IXH60_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1991208.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1715..3046)
                     /locus_tag="IXH60_002318"
     CDS             complement(1715..3046)
                     /locus_tag="IXH60_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1991209.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3201..3602)
                     /locus_tag="IXH60_002319"
     CDS             complement(3201..3602)
                     /locus_tag="IXH60_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1991210.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3688..3894)
                     /locus_tag="IXH60_002320"
     CDS             complement(3688..3894)
                     /locus_tag="IXH60_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991211.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3989..5491)
                     /locus_tag="IXH60_002321"
     CDS             complement(3989..5491)
                     /locus_tag="IXH60_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1991212.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5509..5964)
                     /gene="argR"
                     /locus_tag="IXH60_002322"
     CDS             complement(5509..5964)
                     /gene="argR"
                     /locus_tag="IXH60_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1991213.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(6062..>6380)
                     /locus_tag="IXH60_002323"
     CDS             complement(6062..>6380)
                     /locus_tag="IXH60_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991214.1"
                     /translation="QLMIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCA
                     QILHVGPYAEEPATFEKLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKI
                     EKG"
BASE COUNT         1665 a         1406 c         1047 g         2262 t
ORIGIN      
        1 tttatttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct ctccactaaa
       61 cggtgttcaa tcagctaacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta
      121 ttttgccgtt tgtttctcta atactcaggt caaagcgctg atttcacaac ctctaataca
      181 cggtgttcca gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag
      241 caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat
      301 tattaaagaa ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta
      361 agtagtgata gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc
      421 aacgctttgg cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg
      481 atattttgtt tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat
      541 tcttcatcca ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg
      601 atctgtgtct catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc
      661 tgttttccag catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca
      721 tggatcaccg ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt
      781 cctgtatcag aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa
      841 ataagatctt tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt
      901 catttctcat agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa
      961 aaaagactgt gccaaattat ctgacacagc cctcactata aaactgttct catatagcta
     1021 gatgttcatt gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct
     1081 ctaatacttc ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg
     1141 catccattgc ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc
     1201 cactctctac caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa
     1261 gaattactgt gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc
     1321 caaagatttt atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt
     1381 cgataaaatc ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca
     1441 tctgctgctc aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg
     1501 ggtaaaaata atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca
     1561 tttcaatcat attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc
     1621 caccgcctgc tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt
     1681 tattattgaa catggtggat tctccattcg ttatttattt taatacatca tttagagctt
     1741 cactcattgt cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat
     1801 tcatagctag cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc
     1861 ctagaatcaa gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta
     1921 atacttgtgc tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt
     1981 gtgctttggc ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac
     2041 tgtaaggcac agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca
     2101 taatgattcg ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc
     2161 caatcgccca aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc
     2221 gatctgttag tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc
     2281 cagttgcagc caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg
     2341 tgacggcagc tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt
     2401 cgattcccgc atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg
     2461 gaagaaattc agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg
     2521 atgcaaattc caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt
     2581 cctgatccat cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg
     2641 gtaaaatgat tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt
     2701 tttcaccatt attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca
     2761 tcactgtccc agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg
     2821 tcaatttttc tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa
     2881 tcaatgattt agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg
     2941 acttttcaac gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac
     3001 cgccctttcc aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc
     3061 ttttttacct accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc
     3121 atctgcttta tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg
     3181 gttcgatttc ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt
     3241 gctttggagt aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg
     3301 ctgatgccat cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa
     3361 ttataaagag agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc
     3421 atatgttgta ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga
     3481 tttgttcctt ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt
     3541 gcctcgactt ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc
     3601 attgtactgc tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa
     3661 gtaaggcgct ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat
     3721 ttttccaaaa aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata
     3781 aatagcaatc ttttctacat aatcttttat atgattgatt ttaagataca tatataccag
     3841 aaagttgacc ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc
     3901 ctccttttgc tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc
     3961 aagcaactag cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa
     4021 aatgtccgaa taattttgct aattttacta acgccaactt gttctttccg aattcatcat
     4081 tttctatctg taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt
     4141 ttccgtatgg ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca
     4201 tccctttttc ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc
     4261 gtggttcatc gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact
     4321 ctttgatttg ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga
     4381 tctttgcgat gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc
     4441 cgcttgtttc gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat
     4501 tgattacatt tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga
     4561 atctttgtag atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt
     4621 ttaatacgat tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag
     4681 cactgacaac tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat
     4741 ttgctaaaac agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat
     4801 gattaggaaa tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc
     4861 tattcaatac tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt
     4921 tccaaccatt tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta
     4981 aatcttgcgc ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt
     5041 ttattttttg ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg
     5101 aggtaaagaa ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg
     5161 cagcatcgca cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta
     5221 attcgaaatc gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca
     5281 gtgctggtcc gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat
     5341 cggttgattt cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca
     5401 tgtctgtcgt caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg
     5461 atccattttg cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat
     5521 tttttccagc ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa
     5581 tgtatctaca ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt
     5641 cgttaccaca tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg
     5701 gatacgcaaa acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga
     5761 tggctggtta aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt
     5821 catttctcga atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag
     5881 ccgcgcaagc agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag
     5941 tgcttgtcgc tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat
     6001 aagtaatatt tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt
     6061 gctaaccttt ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc
     6121 ttggatcgct gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg
     6181 cgatgaatgc ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta
     6241 agatttgggc acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt
     6301 ccggaagttt gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag
     6361 gctgtcggat catcaattga
//
LOCUS       DACEZN010000143         6376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEZN010000143 DACEZN010000000
VERSION     DACEZN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.143"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(45..779)
                     /locus_tag="IXH60_002324"
     CDS             complement(45..779)
                     /locus_tag="IXH60_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1991215.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IXH60_002325"
     CDS             1106..1516
                     /locus_tag="IXH60_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1991216.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IXH60_002326"
     CDS             1509..2210
                     /locus_tag="IXH60_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1991217.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IXH60_002327"
     CDS             2210..3796
                     /locus_tag="IXH60_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1991218.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IXH60_002328"
     CDS             3815..4036
                     /locus_tag="IXH60_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1991219.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IXH60_002329"
     CDS             4033..4794
                     /locus_tag="IXH60_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1991220.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IXH60_002330"
     CDS             5174..5683
                     /locus_tag="IXH60_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1991221.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IXH60_002331"
     CDS             complement(5758..6297)
                     /locus_tag="IXH60_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1991222.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
BASE COUNT         1983 a         1170 c         1296 g         1927 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaa
//
LOCUS       DACEZN010000144         6350 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEZN010000144 DACEZN010000000
VERSION     DACEZN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.144"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            257..532
                     /locus_tag="IXH60_002332"
     CDS             257..532
                     /locus_tag="IXH60_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991223.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="IXH60_002333"
     CDS             545..2623
                     /locus_tag="IXH60_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1991224.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="IXH60_002334"
     CDS             2783..4669
                     /locus_tag="IXH60_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1991225.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="IXH60_002335"
     CDS             4681..5628
                     /locus_tag="IXH60_002335"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1991226.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="IXH60_002336"
     CDS             5648..6175
                     /locus_tag="IXH60_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1991227.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..>6350)
                     /locus_tag="IXH60_002337"
     CDS             complement(6238..>6350)
                     /locus_tag="IXH60_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1991228.1"
                     /translation="GAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         2115 a         1043 c         1378 g         1814 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta
//
LOCUS       DACEZN010000145         6243 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEZN010000145 DACEZN010000000
VERSION     DACEZN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.145"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(99..1229)
                     /locus_tag="IXH60_002338"
     CDS             complement(99..1229)
                     /locus_tag="IXH60_002338"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1991229.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IXH60_002339"
     CDS             complement(1878..3080)
                     /locus_tag="IXH60_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1991230.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IXH60_002340"
     CDS             complement(3140..3343)
                     /locus_tag="IXH60_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1991231.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IXH60_002341"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IXH60_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IXH60_002342"
     CDS             4880..5692
                     /locus_tag="IXH60_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1991232.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1849 a         1284 c          995 g         2115 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaa
//
LOCUS       DACEZN010000146         6242 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEZN010000146 DACEZN010000000
VERSION     DACEZN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.146"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            294..1424
                     /locus_tag="IXH60_002343"
     CDS             294..1424
                     /locus_tag="IXH60_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1991233.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1516..2271
                     /locus_tag="IXH60_002344"
     CDS             1516..2271
                     /locus_tag="IXH60_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1991234.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2329..4368
                     /locus_tag="IXH60_002345"
     CDS             2329..4368
                     /locus_tag="IXH60_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1991235.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4477..5628)
                     /locus_tag="IXH60_002346"
     CDS             complement(4477..5628)
                     /locus_tag="IXH60_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1991236.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5782..>6242)
                     /locus_tag="IXH60_002347"
     CDS             complement(5782..>6242)
                     /locus_tag="IXH60_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991237.1"
                     /translation="IAIVLNPSAVFHFIGYLITAYLVLLGLLNLFEAYRFKRKTGSWS
                     IGLFSGLFFLILALIVYAFAPAIVSILPILLGLAVIINSLFQLFFSMNTKAKSWSVYS
                     SILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMGIQEIINFIRIRKMTIR"
BASE COUNT         2163 a         1128 c         1236 g         1715 t
ORIGIN      
        1 cctatacaaa cataggctgt tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa
       61 actgttggat taaggctttt tccaatagct aagaatgttt tctttacgtt gtatagtgct
      121 aaaactcgga cgatgagatg atgctaggtc agaattcttg ttcgaaaagg tttctgacct
      181 tttctttata tttttgtgac tgacttattt gatgagacaa actcgctcct cacctgacaa
      241 gtggattgct gtgtgaggag gagatgcggt gaaaaaagat cgattggtgg attttgggtc
      301 cttatttgac tttgtccatg atcggtcttt tagaagtata cagtgccagt tcttatcgtt
      361 tattgcaagc cgatgaaaat acaaaaagtc tattattgcg tcaactaatt ttcatatttt
      421 tgagttggag cgtgatcttc ttagctcgtt cagtcaaact acactattta cttcacccta
      481 aaatagcagg atacggttta gccttatcga ttttcttttt agtattagta agaataggga
      541 tattcggtgt cactgtcaac ggcgcacaac gttggatctc tctgtttggc attcaattcc
      601 agccttctga actggcaaat ctttttttga ttttttattt aagctggttt tttcgtgacg
      661 gaaatagtag cccaaaagat ctaaaaaaac cattcctgat tacagtaggt ataacttttc
      721 tgattttatt tcagccaaag attgctggag cattgatgat cctttcgatt gcgtgggtca
      781 tattttgggc agcggcggtt ccatttaaaa aagggatcta tctaatcgtt actttttctg
      841 cattgctgat tggagcagca ggcggggtat tatatttagg aaataaaggt tggcttccac
      901 agatgtttaa tcatgcttat gaaaggatcg cgactttaag agattctttt atcgatagtc
      961 atggtgcggg ctatcaaatg acccattcgt tttatgcact atataatggt ggcatatttg
     1021 ggcggggatt aggaaatagt atcacaaaaa aagggtatct tccagagaca gaaacagatt
     1081 ttattttttc gattattaca gaagagcttg ggctaatcgg tgcattgtgt gtgcttttct
     1141 tattgttctc attatgcatg agaatctttt gtttaagcag tcgctgtaaa aatcagcaag
     1201 ctggattgtt tcttttagga ttcggaactt tgctattcgt tcagacgata atgaacgtgg
     1261 gaagtatcgc ggggctgatg ccaatgactg gtgtgccatt gccatttgtt agctacggag
     1321 gaacaagtta tctgatcctt tctttaggaa tcggaatcac gttgaatatt tcttcaaaga
     1381 tacaggccga agagttggcg ctctatcgac ctgaaaaaca ataaagagaa actgagaaac
     1441 tctcagtttg aattatcata aagtacaatc ttttattttt caagcgagag gttcttttag
     1501 aaagaggaat tcgcaatgaa agtatctcaa aaaatcatat tatctatttt gttactgctt
     1561 acattagtag ttggctattt ttcgattgag tttatccatg gattttcgtc tgcaaaacag
     1621 agttcaactg tgaaaaaggt agagccgaaa agtatcccta ccacactaaa tgtggctttg
     1681 attggttcag atgctcgttc gaaagacgaa aacggtcgct cggattcact tctggttgca
     1741 caatacaacc agaaaacaca acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc
     1801 gatattccag gttacggaat gaataagata aatgcagctt actcttatgg gggagttgat
     1861 ttattaaatc aaacattaaa ggaaaatttc aaatttgaag ctccctatta tgcaagtatc
     1921 acatttcaag attttatcga ttgcgttaat gaactgtttc ctggtggagt caagatcgat
     1981 gcagaaaaat ctctagattt agatggcgta tatataaaca aaggagagca agtgatggat
     2041 ggtaatactt tactccagta tgctcgattt cgggaagaca aagaaggaga cttcgggagg
     2101 atcagaaggc agcagcaagt gatcaaagct atctctcagc agctgaaaga cgtaacttca
     2161 atattcaagc tacccaaagc ggttgggaaa ttacttggta gtatccaaac gaaccttcca
     2221 gaaagtgtgc ttctggattg tggtatggat ttcctcaaag acatatgtta gaataaacag
     2281 gtataaatag tgaaataaag gaatgacaag caagagaagg aggaaaaaat gaaaagaagt
     2341 gacaagcacg gcaaaaatcg aacaggcgct tatattgccg gcgcagtgat cttaatagca
     2401 gctgcgggtg gcggttattt ttactaccag cactatcaag aaacccaagc agtagaagct
     2461 ggagaaaaga cagttgagca atttgtccaa gctttgaaca agggagatta taacaaagct
     2521 gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat tatctgaaaa agagatctta
     2581 gataaatacc agaatatata cggtgctgct gatgtcaaag gacttcagat atcgaatcta
     2641 aaagtagata aaaaagacga ttctacttac agtttttcat ataaagcaaa gatgaatacc
     2701 tcattaggtg aattgaaaga tctttcttat aaaggaacat tagacagaaa tgatggtcaa
     2761 accacgatca actggcaacc caacttggtt tttccagaaa tggaaggaaa tgacaaagta
     2821 agcctgacca cacaagaagc agcaagaggg aatattatag atcgaaatgg tgaaccatta
     2881 gcaacaacag gcaaactaaa acaattagga gtcgttccaa gtaaacttgg agatggaggc
     2941 gaaaaaacag ccaatatcaa agcgattgct tcctccttcg acttaacaga agatgctatc
     3001 aatcaggcga tttcacaaag ctgggtacaa cccgattact ttgtcccatt gaaaatcatt
     3061 gatggagcaa cgccagaact tccagctgga gctaccattc aagaagtaga cggcagatat
     3121 tatcctttgg gtgaagcagc tgctcagctg attggttacg tgggagatat cacagcagaa
     3181 gatattgata agaatccaga attaagcagt aatggaaaaa tcggtcgctc aggtttggag
     3241 atggcttttg ataaggatct tcgtgggaca acaggtggga aattaagcat tacagatgca
     3301 gacggtgtag agaaaaaagt tctgatcgaa catgaagttc aaaatggcaa agatatcaaa
     3361 ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca gtctaggagg aaaagctgga
     3421 tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg cgcttgctag ctctccaagc
     3481 tatgatccaa acaaaatgac aaacgggatc tcacaagaag attacaaagc ttatgaagaa
     3541 aatcctgaac atccattcat cagccgattt gcgacaggtt atgctcctgg atctacgttt
     3601 aaaatgatta cagcagcaat cggtctcgac aacggcacta tcgatccaaa tgaagtgttg
     3661 acgatcaacg ggcttaaatg gcaaaaagac agttcttggg gatcttatca agcaacgcgt
     3721 gtcagtagtg atgtatcaca agtagactta aaaactgctt tgatctattc cgataatata
     3781 tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa
     3841 ttcatttttg gtgaagacct tgatttgcca attagtatga atccagcaca aatttctaat
     3901 gaagatagct ttaactcaga tatcttgcta gctgatactg gatatggaca gggcgaactt
     3961 ctaattactc ctatccagca agcagcaatg tattctgttt ttaccaacaa tggcacactt
     4021 gtctatccta aattgattgc agataaagag acaaaagata agaagaatgt catcagcgaa
     4081 acagcagtac aaacgatcgt gccagatctg agagaagttg tgcaagatgt aaatggtaca
     4141 gcacattctc tttctgcttt agggattccg ttggcagcga aaactggtac agcggaaatc
     4201 aaagaaaaac aggatgtaaa ggggcaagag aacagtttct tgtttgcttt caatcttgat
     4261 aaccaaggtt atatgatggt cagcatgttg gaaaataaag aagatgatga ttcagcaacc
     4321 aaacgagcat cggaactatt gcaatacctc aaccaaaatt atcaataaaa aattgctttt
     4381 taagtacagt aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt
     4441 tcacgatgtc tctttcacaa cttctttttg atttactcac acttcataaa ctttttttgt
     4501 aaaatatttg agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg
     4561 tgaatagtac tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc
     4621 catttctcca cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc
     4681 ggcaatctga gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa
     4741 tatagaaata aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact
     4801 gacaaaaaat actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc
     4861 ttctttaacc tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct
     4921 aagcccaaca agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat
     4981 aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt
     5041 gactaataag aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga
     5101 agcattgcta gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac
     5161 taaaatgtca tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac
     5221 gtttagttct tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc
     5281 aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac
     5341 aaccgggaag atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa
     5401 aagagataaa tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga
     5461 aaagtcatgg actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac
     5521 accaacaagt aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc
     5581 aatcgttgtt gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc
     5641 tttgtataaa atatagcccg aacttagcat aacatacatt attgaaaaaa taggaaaata
     5701 tactcacaaa aaaaccaaca atccaaaaga tttttttgga ttgttggttt ttatacggtc
     5761 tattttaata actaaaaatg attaacgtat agtcattttt cttattctga tgaaattgat
     5821 aatctcctgg atgcccatga aaatcaaaat aaagccgaat atttggaata gaagcatcaa
     5881 acttttgaat ggattgaaca ataagacgaa tccgccaatc aacaacagaa tactactata
     5941 aacactccaa cttttagctt ttgtattcat gctgaagaac aactggaaca agctgttgat
     6001 gataacagct aatccaagaa gaatcggcag gatagaaacg attgcaggtg caaaagcata
     6061 gacgatcaga gctaagatca ggaaaaaaag accactgaac agaccgatac tccaagaacc
     6121 tgttttcctt ttaaaacggt aagcctcaaa aagattcaac aacccaagca aaacaagata
     6181 ggcggtgatc agataaccga taaaatgaaa aacagcagaa gggtttagca caatcgcaat
     6241 cc
//
LOCUS       DACEZN010000147         6222 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEZN010000147 DACEZN010000000
VERSION     DACEZN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.147"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            352..1041
                     /locus_tag="IXH60_002348"
     CDS             352..1041
                     /locus_tag="IXH60_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1991238.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IXH60_002349"
     CDS             1028..2236
                     /locus_tag="IXH60_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1991239.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IXH60_002350"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IXH60_002350"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1991240.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IXH60_002351"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IXH60_002351"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1991241.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1913 a         1168 c         1208 g         1933 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aa
//
LOCUS       DACEZN010000148         6136 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEZN010000148 DACEZN010000000
VERSION     DACEZN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.148"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(207..1169)
                     /locus_tag="IXH60_002352"
     CDS             complement(207..1169)
                     /locus_tag="IXH60_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1991242.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IXH60_002353"
     CDS             complement(1171..2610)
                     /locus_tag="IXH60_002353"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1991243.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IXH60_002354"
     CDS             2795..4741
                     /locus_tag="IXH60_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1991244.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="IXH60_002355"
     CDS             5005..5877
                     /locus_tag="IXH60_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1991245.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1947 a         1055 c         1083 g         2051 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 atatctttgc ttcttc
//
LOCUS       DACEZN010000149         6128 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEZN010000149 DACEZN010000000
VERSION     DACEZN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.149"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(63..1268)
                     /locus_tag="IXH60_002356"
     CDS             complement(63..1268)
                     /locus_tag="IXH60_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1991246.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IXH60_002357"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IXH60_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IXH60_002358"
     CDS             complement(1447..2832)
                     /locus_tag="IXH60_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1991247.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IXH60_002359"
     CDS             complement(2861..3247)
                     /locus_tag="IXH60_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1991248.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IXH60_002360"
     CDS             complement(3263..3577)
                     /locus_tag="IXH60_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1991249.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IXH60_002361"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IXH60_002361"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1991250.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IXH60_002362"
     CDS             4837..6126
                     /locus_tag="IXH60_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1991251.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         1770 a         1243 c         1154 g         1961 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaata
//
LOCUS       DACEZN010000150         6017 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEZN010000150 DACEZN010000000
VERSION     DACEZN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.150"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..986
                     /locus_tag="IXH60_002363"
     CDS             <1..986
                     /locus_tag="IXH60_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1991252.1"
                     /translation="RRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYD
                     KENKEILLIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKE
                     EFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGN
                     QPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLN
                     PNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSS
                     ISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1078..1443)
                     /locus_tag="IXH60_002364"
     CDS             complement(1078..1443)
                     /locus_tag="IXH60_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1991253.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1440..1805)
                     /locus_tag="IXH60_002365"
     CDS             complement(1440..1805)
                     /locus_tag="IXH60_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1991254.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(1997..2245)
                     /locus_tag="IXH60_002366"
     CDS             complement(1997..2245)
                     /locus_tag="IXH60_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991255.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2244..2372
                     /locus_tag="IXH60_002367"
     CDS             2244..2372
                     /locus_tag="IXH60_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1991256.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2429..4123
                     /locus_tag="IXH60_002368"
     CDS             2429..4123
                     /locus_tag="IXH60_002368"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1991257.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4205..4396
                     /locus_tag="IXH60_002369"
     CDS             4205..4396
                     /locus_tag="IXH60_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991258.1"
                     /translation="MDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKVV"
     gene            4774..5907
                     /locus_tag="IXH60_002370"
     CDS             4774..5907
                     /locus_tag="IXH60_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1991259.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
BASE COUNT         1984 a         1148 c         1107 g         1778 t
ORIGIN      
        1 aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc
       61 tacctgaatc gttttttgaa cgtgccgatc gtttcattga atccgtaacg gaaaatagtg
      121 gcagatttta tgataaagaa aataaagaaa tcttattgat tggttttgct atcagtttcc
      181 tacgccagca agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac
      241 gtcctattta caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata
      301 aaaaagaaga attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt
      361 cttatcagtt gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc
      421 ccgaaataaa agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa
      481 accaaccatt cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat
      541 tattcatgcc agaaaaattt tatcttctaa atgaggaaca gacaaattta tataaagaag
      601 tccagacaat tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta
      661 attgcatgcg ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc
      721 tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg
      781 aagctgtaac tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata
      841 taaagaagta tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac
      901 cagatgcggt ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta
      961 tcatttcagc tgtgcaaaac aaataaggga ctgtgacaaa aatcatgtca cagcctctta
     1021 tttgttgaag atgcccattt ctgtaacaat ctcttgatac acatcaggct cacaacctca
     1081 cttattttgg acatctacgg gtccgatctc tttatttaaa aaataagaaa tgatggttcg
     1141 gataactacc aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc
     1201 gatgatccca gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt
     1261 tttaatagca ttgttctcgc tgactcggtc gctttgcact tttgtatgga caaataaata
     1321 tttcaaaaaa tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat
     1381 atcaagtccc agtacaagtt tttcaaaata tggaagaaga aactgcatca actctgtcgt
     1441 catcctacct ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa
     1501 gaaatgactg ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc
     1561 ttgataactg actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa
     1621 cttgctagca gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg
     1681 acatgagacc aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg
     1741 atagaacaaa tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga
     1801 aacatatacg ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt
     1861 taccttgttc gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt
     1921 ttcactctcc cctttttctc cctccatcca taaatcgtgg aagtatccaa aacaacgtag
     1981 caagttaaaa taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt
     2041 tttttgtacc caaatttcta aaaatagaac catacacctc ctcaaatctt ttcttcttac
     2101 ctaccctatt tttatttaca ggaaaatcag acacttcttc ttttttgtca aaatttcctt
     2161 tttcttctcg tcctatcatt tggcattcta ataaaaacga actatccttg gatatagaaa
     2221 gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat caaagtcggc atcggtttag
     2281 gtttgcttgc ccttggcgct gcatatctag gaaaaaagac aggactattc gaagatgaca
     2341 gtcatttata tgatgaattc gaatcaatct agcatcctat ttagttgaat cactacggac
     2401 atacttggaa gacattggag gaatcacaat gagaaaaagt aaagcaataa tgattggtgc
     2461 cggtattgcc aatatggcag cggctgttta tttgattcaa gaaggcaaat ggaaagggga
     2521 tcagatcacc ttttattcat tggatgatca cggttcaaat gatggtgcac cgaccgacac
     2581 tgtagtggat gaatattgga ataaaaacca tccattagaa aaccaaaaag gctatattgc
     2641 tcgaggcggt cgcatgctta attaccgtac ctatgtagat ttgatggatc tgttaagtcg
     2701 aataccttct gttactgagc cagggatgac tgctgaagaa gatacgagag attttgatgc
     2761 aaaacaccga acctttgaca aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg
     2821 cggaaaactg ggattcaata ataaagaccg tattcttctg actaaactga ttgctatgcc
     2881 tgattcagaa gaagaaaaac tagacaatat cactatcgaa gaatatttca aagatgatcc
     2941 acactttttt gaaacaaact tctggtttat gtgggaaaca acatttgctt tcagaacacg
     3001 tagttctgct caagaattgc gtcgctatat gcatcaaatg atttatgaat tcactcaaat
     3061 cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc
     3121 tttgatcgag tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga
     3181 ctgggaattc aaagaaacac cgatgcaaga tgaaatcact gtgactggtt tgaaaatgac
     3241 caatacactg actcaagaag aagaacacgt tgtggtcgat gaagatacag cagttatttt
     3301 cactaatggg tccattacag attcagctac tttaggagat tacgaaacac cagctgtaga
     3361 aaatatggat tatggtgcag cttctagtct ctggaaaaaa gccagtgaac gtttctacaa
     3421 cttgggaaat cctgacaaat tctttgctga tcgagatgca agcgaatggg tcagctttac
     3481 cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc
     3541 aggaaatgcc ttgaattcat ttatttcaac cacaccgatc acgccgttag gccaaaaaga
     3601 tgtcaatatg tctatcgtcg tgcatcatca accgcatttt acgtctcaga aaccaaatga
     3661 aacagttatt tggggttatt tcctttatcc aagacgacgt ggcgaatttg tggataaaca
     3721 gtatattaaa atgaatggaa aagaaatgct agaagaactg attggacaac tttcaaaagt
     3781 agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa
     3841 taatatccct gtctatatgc cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga
     3901 tcgaccaaaa gtcattccta aacattcgac caatctggcc tttacaggag aatttgtcga
     3961 gcagccttat cagatgatct ttactgaaca aagtgctgta cgttctggag aaatcgctgc
     4021 tttccatttt gcgggtatcc ctatgtcacg tcttgtcaaa acaccgcgtt acgacaaaga
     4081 tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa
     4141 agctgccctg catcaagtaa taagaacaac caggctaaaa cagcttctta tgtttttgat
     4201 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttgtga
     4261 aaaagctgcc ctgcatcaag taataagagc agccaagcta aaacagcttc ttatgttttt
     4321 gacttggttg gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
     4381 tgaaaaagtc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt
     4441 tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac ctctttttat
     4501 ttatgtctgt ttctttttga aaacaaccat tttttcaacg tttactctct tgccaatcca
     4561 acatcatcta acagatacaa ctatttcaat gattaccatt acactaaaaa atcggaaact
     4621 cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa aaaaagcgtt
     4681 tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac taataatgca
     4741 gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa
     4801 cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac agatgcaatc
     4861 atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca tcatggtgaa
     4921 gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg
     4981 ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt taatattagt
     5041 tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga atcaaatcca
     5101 gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca ttggggtggt
     5161 caagcggaat atctaagagt tccttatgca gactctactt catttgttgt accagataat
     5221 gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc ttattggagc
     5281 gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc tggtccagtc
     5341 ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat cgcagtagat
     5401 ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac ttatttctta
     5461 gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc
     5521 attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc gaaaaattta
     5581 gtaagcttac aaaacggcac aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa
     5641 tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta cccgctgcaa
     5701 gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat ccatctaatg
     5761 ccaaaaattt acgaaatgat tgcagaagga ctattcgatc ctacacaaat cattactcat
     5821 tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa agaagataaa
     5881 aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat tttctttatg
     5941 cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga tatcgaaata
     6001 cgaggacgtc gcttccg
//
LOCUS       DACEZN010000151         6009 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEZN010000151 DACEZN010000000
VERSION     DACEZN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.151"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..329)
                     /locus_tag="IXH60_002371"
     CDS             complement(<1..329)
                     /locus_tag="IXH60_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1991260.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTV"
     gene            complement(418..1599)
                     /locus_tag="IXH60_002372"
     CDS             complement(418..1599)
                     /locus_tag="IXH60_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1991261.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(1835..3343)
                     /locus_tag="IXH60_002373"
     CDS             complement(1835..3343)
                     /locus_tag="IXH60_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1991262.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            3502..3693
                     /locus_tag="IXH60_002374"
     CDS             3502..3693
                     /locus_tag="IXH60_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991263.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            3896..4813
                     /locus_tag="IXH60_002375"
     CDS             3896..4813
                     /locus_tag="IXH60_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1991264.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            4806..5879
                     /locus_tag="IXH60_002376"
     CDS             4806..5879
                     /locus_tag="IXH60_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991265.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
BASE COUNT         2046 a         1119 c         1101 g         1743 t
ORIGIN      
        1 accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa
       61 tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt atgttgagca
      121 tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt attgtcagaa
      181 acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta
      241 atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc agaaccagaa
      301 ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat tgaacaagtg
      361 aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta
      421 taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg ttagcaaaat
      481 tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct tcatcgcacc
      541 agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag atataattgc
      601 tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag caacaaattc
      661 atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt tccatgggat
      721 tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga aagtgatgcc
      781 gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac cgataagcat
      841 agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca acatttggaa
      901 aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt ctaatacata
      961 gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa gggcagtgat
     1021 cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag aaactgtaga
     1081 catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta attccttttt
     1141 tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat tataagattc
     1201 caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca taaataatgg
     1261 aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa ttggcatcaa
     1321 tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa caaagtcaac
     1381 accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag aaacaaccgc
     1441 tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa cgaacatcac
     1501 aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag caagccctaa
     1561 tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc tattccaaat
     1621 tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa ttttcattac
     1681 ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa aataaaaggt
     1741 ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga ttaagctaat
     1801 agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga ttttcggaag
     1861 ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt tcatcatttt
     1921 tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt ttatcataag
     1981 caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag acttcattga
     2041 tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt attgtttcaa
     2101 gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat
     2161 ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta atgatgactt
     2221 catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt tgcgcttgaa
     2281 tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga cgtaaaccga
     2341 cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat ccttttttga
     2401 agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct tcttggacaa
     2461 cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta ggagattgat
     2521 aaacagattc tttaccagta attttttcaa taatacgttt gattacagga aatgtctcaa
     2581 catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag gaatcgatca
     2641 tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc ggatgtttca
     2701 acggaacatt ccatactggg aaagtttcga acttggagta accagctgca tgtccttttt
     2761 ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct gggccaggag
     2821 ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca aaaccatctt
     2881 ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt ttatagacct
     2941 tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt ccactatagc
     3001 gagtgataac gacactgttg attaataagc cataaccacg taattcatcg attaaccgca
     3061 agatatccat atcataagta atcccataat caccacggat cttattacgt tcaatatctc
     3121 cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc aatttgattt
     3181 tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca actagcttcc
     3241 caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata tacgctgact
     3301 gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc cctccttaag
     3361 tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga aattcctcaa
     3421 aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg gtatgatagt
     3481 ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc atataaacac
     3541 tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga attggaacaa
     3601 ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca tcagcgcatt
     3661 catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc atcggattcg
     3721 attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac caaattttgg
     3781 tgcagcctgt tttatggagc attgcccgct cttttttata acaatttttt acagaaaatg
     3841 gtactattga agtattaata agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa
     3901 agagccagat acaaatgcaa attatgagcc agacgaacta gaggagacga atgcggatca
     3961 ggatacagga caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct
     4021 ttcccaatta ttagaagact tacaggcttt tgaaaaagct gtccgcgaag aagatatccc
     4081 tgagatctac cgtttataca atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga
     4141 aaatcacgag atcgatcaat tgctgatgcg taagatccat gatcgttttt tacaggaatt
     4201 tccttttatg gaacaagtag aaaacatctc gccaacgatg agctattaca aaatcggcac
     4261 ttattaccat gatcggccca cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt
     4321 gcctaagatc gatgaagagt gggagaaata ttcccaaccg gagtcaacgg actatgacaa
     4381 aaaaatcaat gagctagacg caaaggtgat cactgctcag acagaaattg aacgtttaga
     4441 tgaacaaata aaagaaatca atcgacaaat gactgatttg aacgacaata aaggattctt
     4501 gaatcgtaaa aaaatcgagg aagaaattca agaattagag aagaagaagc aagtactatc
     4561 taatgaaaaa ttaggctggc ttccttatat tgagacgcct gaaacaatcc agcagcaaaa
     4621 agaagcattg aagcaagaag caagagcaga ccagcttcgt gcagccatcg tagaaaaaga
     4681 acaaaggcaa atcagacgct atttcggtag caaagaggga ttcggtcaag caatccatga
     4741 attcttgatg aattatcttg gcaatgagaa tcctaataat caatctaatg aaggaggttc
     4801 cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc
     4861 atgaagaaac gaacacttca ttggaagaac aaacaagtaa tcctctggat gacacagaag
     4921 aaatccctat gctagaggaa acaaaaatac acgaaaaaga aaaagtaact ccgacgaata
     4981 cgaatactgc agaaaacacc catgcttacg aaataactga aaatcccggg tatttagaag
     5041 gaaaagagca agatcatcaa gatatcgtca gtttgattga aacagcggaa gcagaattag
     5101 cggatttacg tatgagaaaa gatttgatcg aatcagattt tccacaatta ttagaatcat
     5161 atgatcgttt tatattgaat gaagaaccaa tctccacgat cggaaagaca agcctgatcg
     5221 aatacttcac gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt
     5281 cagcttatga cacatggaaa acaagacatt tttcgatcaa ttatacgttg aatggacaaa
     5341 acgaattagt cttttccttt gtattaccga ccatcgatca acgataccaa gtggaatcga
     5401 tgagtctcct aaacgtatca ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc
     5461 tttctttgat ccgacactgg tcagtggatc gagtattctc tgcagggcag atcacgatat
     5521 tcaatcacaa attgaatcaa ttattagccc atgcaagaac acttggtttt accgttaatc
     5581 agacattact agacaatacg aagccacttc gtttgaatct tcagagcgaa tttgaattga
     5641 cgaatgaagt attggatgat atttttatcg tggcaatgaa aaatccatcc tacgatatgg
     5701 aaaagatcaa cgaaactact tatcaagtac gattagataa aggacagtct ttaacgataa
     5761 caacaaacga aaaagatcaa acagtcttgt cgatctcatc tggagactac ccacgatctg
     5821 tcattgattt ctttatcaat tacgaattct tggtcccatt aatggtgaga aagtcctgat
     5881 tggataagtt ggattaccag atgtcattca gataattctg ttccaaagga cttgcggcag
     5941 ttatccagaa taattgtcac aagtcttttc tgtatgtact aaatggacgt gaataacttg
     6001 ctgatgaag
//
LOCUS       DACEZN010000152         5905 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEZN010000152 DACEZN010000000
VERSION     DACEZN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.152"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(101..925)
                     /gene="nadE"
                     /locus_tag="IXH60_002377"
     CDS             complement(101..925)
                     /gene="nadE"
                     /locus_tag="IXH60_002377"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1991266.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(937..2409)
                     /locus_tag="IXH60_002378"
     CDS             complement(937..2409)
                     /locus_tag="IXH60_002378"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1991267.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3046..3408)
                     /locus_tag="IXH60_002379"
     CDS             complement(3046..3408)
                     /locus_tag="IXH60_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1991268.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3631..4383
                     /locus_tag="IXH60_002380"
     CDS             3631..4383
                     /locus_tag="IXH60_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1991269.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(4523..5446)
                     /locus_tag="IXH60_002381"
     CDS             complement(4523..5446)
                     /locus_tag="IXH60_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1991270.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(5461..5733)
                     /locus_tag="IXH60_002382"
     CDS             complement(5461..5733)
                     /locus_tag="IXH60_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991271.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1769 a         1190 c          976 g         1970 t
ORIGIN      
        1 tatcaaatta aaagtttact acagctgata tttttctttt ctattttata aaacaaaaac
       61 agccaatccc tatgaaggga cggctgtttt tgctttattg ttatttccag aaatcatcca
      121 aaacggaaat tggcaaatgt cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt
      181 cttgtgcttg agcgggaact tcttttcctt ctagataatc atcaatttca ttatatgtga
      241 cacctagtgc gacttcatct gcaatcattg gtttcccatc ttcaagatct gcagtcggaa
      301 tttttgtgta taatttttcc ggtgcattca atgcttgaag caattgtttc ccttgtcgtt
      361 tgtccaaacg gaataatggc aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc
      421 cagtaatgtt ttctgcagca tgatctgtcc ctaatacggc tcctgctctt tctccagcta
      481 cggcatattg cgtaatcata cgttgacgtg ctttgatatt tcctttgtta aaatcgctga
      541 ttcccacacc tgcttcgctt aatacttgta cttgtgcatc aacagctggt ttgatattta
      601 ctcgcagact aacatcgggc tggataaagt caagagcagc ttttgcatct tcttcatctg
      661 cttgttcacc ataaggaaga cgaacagcga taaattgata gttttcatct tttgtttctt
      721 cacgcatttc ttccatcgtt agttgcgcca aacggcctgc taaagtagaa tcttggccgc
      781 cgctgatacc tagtacaaaa gttttaagaa acggatattt tttcaaatat gctttcataa
      841 agtcaatact tttacgtatt tcttcttttg gatcaattgt tggtttgaca cctaattcat
      901 tgataatttc tgtctgcagg ctcatttatt ctcctcctat gcttctgtat gaagttctgc
      961 tacgcttttt ttcattcgtt ccatgatagc cattttatgg ttccagcatt cagtagacag
     1021 atcgactggg tatttttgcg gattcaagtc tcgtttatac tcttcccata gtgaatctag
     1081 attttctttt gtatattctt tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc
     1141 ttcgataaag atatcttgta aaactgggcg agctgtaaag tctcgaacag ttttattgat
     1201 aaacgtatgc actggatgga acatgaagat agcttcttct tctcgagggt cttcatccca
     1261 taatgtcacg tagtctcctt cagatttccc atcaaaattt cgagtgatgc gccacacttg
     1321 ttttttccct ggagtagtta ctttttctgc attgcttgat aacttgatcg tatccatcat
     1381 ttttccttca tcatcttcga tcgatactag cttgaatact gcaccaaggg ctggctgatc
     1441 ataggctgtg atcaattttg tcccaacacc ccatacatca attttagctt tttgcatctt
     1501 taaattcaaa atcgttgctt catccaagtc attcgaagca tagatttttg cttccgtaaa
     1561 tccggctgca tctaattgtt cccgaacacg cttagatata taggccatat ctccactgtc
     1621 gatgcgtacg ccttggaagt tgattttgtc acctaattct ttcgccacgc gaatcgcact
     1681 tggcacacct gatttcaacg tatcataagt gtcgactaag aacacgcagt ctttatgggt
     1741 tttagcgtag gccataaatg cttcataatc atttccataa gattggacta acgaatgagc
     1801 atgggtcccg ctagctggta tcccaaatat cttcccagca cgtacattgc ttgttgcatc
     1861 tgctccgccg atatatgccg ctctagttcc ccagactgct gcatccagtt cttgtgcccg
     1921 tctagttcca aattccaata gtggatcttc tccgatgaca gatttgatac gagctgcttt
     1981 tgtcgcgatc aacgtttgga aattgaccat attcaacagt gctgtttcta ctaattggca
     2041 gtgcgctaaa ggcccttcca cttggattaa tggttcattt gcaaaaacta gttctccttc
     2101 acgagcagaa cgaactgttg ctttaaattc aaaattttgt aaatactcca aaaaaccttc
     2161 aggatagact tctaatccac gcaagtaagc aatatcggaa tcattgaacg tcagattctc
     2221 taaataattg actaaccgtt ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat
     2281 ttctcgaaag taacattcaa aaacagcatg taaatcagct cgacctaatt cccagtatgt
     2341 ctgcatcata ttgatttgat atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt
     2401 attattcatc ttgtattact cctcttggca agttttcatt tcctacccat tataacaaaa
     2461 aaaattaata ggaaaagaaa tcttccattt tttctgaaaa agcacatgtt ttacggatat
     2521 ttcttgctaa aaatgaactc catctgctaa aactatcctt tcttattggt atttaaccta
     2581 aacgagaaag aatacttatt ttattcaatg tatccgagac acgaagaaat tagcgttttc
     2641 tatttttctt acttgataaa ataagtagat tttcctacta caatcgtagc cgttaagata
     2701 ccgatgattg aacaaatata accaaatgtc tctacaacat aatcaatggc aaaagttttt
     2761 gaagcaataa tacgtctttc cttttttgct tcttatcccc aaagaaacgg atataactta
     2821 ttatgtattg aatttttttg gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa
     2881 actcgcggaa aaaatatcac acgtttttaa aaagatacaa agtaagaaag cagctgtcaa
     2941 tagaatggaa acaaaaatgg cgagacatca atcagtctcg ccttgttttt tattgtatac
     3001 attagatatg gagcaggtta ccataaagat gattattagt cgttattaaa tagaatcctt
     3061 cgtatttttt tgttctatca ctacaatatc tttatttgga cgtttgaatt gtattccggc
     3121 acgttctagc tcctgtttca ctcctacatg gactttgaca tcttctcgtg tcatttttaa
     3181 aacgtgatga atcgcatagt cgatttcttg ccttgtgatt tttttcttcg tattgatcat
     3241 cacgacttgc tttttatcat atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa
     3301 taatttgtaa taattaatag ccgtattccc aaaaaagaat tccactactt taggataaaa
     3361 tgtcttaata tttaaattac tattcacagg tgtttcgatt agcttcatct cattcctctt
     3421 ttctatatag tcgatttaga tccaatctta agttatgtta ccatctttcc tagcattaag
     3481 gaatcggtta caattgaacc tttaaagaca aaaggattta ccttttatca cttaatagta
     3541 taacataatt tcgtgagaaa aaaatacaat tttttcttga ccatctcgtt acgtaaagga
     3601 ttatgcttac atttgtcagg aggaaaaata atggagtaca caataaagaa aatgtcagag
     3661 atatcaggag tcagtccgag aaccttgcgc ttttatgatg agattggact gctaaaacca
     3721 gctaggatca attcttcagg ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa
     3781 cacatcttat tttatcgaac aatggcattc aagttggatg atatccaaga ggtcttagac
     3841 aatccatctt ttgaccacca gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa
     3901 cgggcacaaa tcgataccct cttgacaact gtccaacaga cacttgatat gtacgaagga
     3961 ggaagaaaaa tgagtgacaa agaaaaattt gaagggttta aacaacaaaa gttgaaagaa
     4021 aatgaagaat cctatggaca agagattcga caaaaatacg gaaaaaaaaa ggttacagca
     4081 tccaatgaaa aatggatgaa tatggataaa gaaacgtatg aagaaatgca aactgttgaa
     4141 aagcagatgt tatttgatct cactctttac ttaaaagaac cagaagatga aagcttagca
     4201 gaccgtatct tccaagctca taagaagtgg ttctcttaca gctggcctga atatcagcca
     4261 aaagcgcata aaggactagg aatgatgtac gaagccgatg aacggttcac tgcatattat
     4321 gacgagcgaa gtggtcatgg tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg
     4381 taatctttta catgatccaa caaaaaagta cacgtaaaat gacctaataa caaaacagca
     4441 gacacccaag ttagattttt atgctaactt ttggtgtctg ctccattctt tcacaagttt
     4501 tttcatccta ctttaaatat attcatcgtt gaataaacgt ataaggtatt ttcactttat
     4561 cctttttttc tgtaagaaac aattgaaatg ccatttctcc gcaagcttcc agatggtggt
     4621 cgatggttgg gaaaccaaga agctcgctga tcagcaaatt atcgcgtcca atcaccactg
     4681 gtgtcttatc ttgcagatag ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa
     4741 agacaccatc aactccaagc tctttaaaaa agaagcctgc ttgttttcct ttctctgcat
     4801 ctatacaatc ccaaaaaaca agtgattcat caaaagatgg aaaaagttga tgacatagct
     4861 gcaaagtgat ttttgagtta taacttagcc tgccacttcg ccccaacgtg atgcccaatt
     4921 gctttatacc ttgctgcttc aaataagtaa gaccttctgt aattgattgt tctcgatcaa
     4981 taaagacaca gcttgcctgt tttcccttga tttcttcaca aaaaactaga ggtccatatt
     5041 tttgatatgc caataaatca tctagtgtat tggcacgaga cgtaatgatc aatccatcga
     5101 aagctttagc tgcaaattct tcaagatacc ttcgctcttt ttctttatga tagcccgtcg
     5161 gtaaaagtgt cactttgtaa tcataatcaa acgctgcttc aatgatccca ctgatcaact
     5221 gatcaaaata cggatgattg gtataaggca gaatcactcc aatgcttttc gtttttccat
     5281 aacttaactg tctggcattt gcattaggga tataatcgac ttctttcatg accgcaagta
     5341 tttctttcct tttttcctca tctacatatg gatgtccatt gattacacgt gaaatagtag
     5401 aaactgaata acctgtgagc tttgcgatat ctcgaatgtt tgccactgct gcttcgccac
     5461 ctatacaaaa agattttctt gtaaatagtc taccacagct ggaccgcatt caaaaaactg
     5521 atacattttt ccttcatgcg aaaaactgat ccgctgcccg ccccaatata tatcttttgt
     5581 caaattttcg atttcattca ttggtatttc ttttactata ttcctattca acccttgaaa
     5641 actgtacaac gttaatacgg atcccttgaa ctcgacaaac gcataaaaat aattgttcgt
     5701 tgtgccatct gttatctcta cataagtatt cattgcaagc cctccttttg atactttgag
     5761 tatagtgtat gaaacgcgtc tcatagcaag gagtttgttt tgccaaagaa aagttttatt
     5821 cctctttcat tattaccaaa gtctcttatt ttaaattgaa tacattataa tgatactgta
     5881 aatatatctt tattattagt gaatg
//
LOCUS       DACEZN010000153         5882 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEZN010000153 DACEZN010000000
VERSION     DACEZN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.153"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..2168
                     /locus_tag="IXH60_002383"
     CDS             <1..2168
                     /locus_tag="IXH60_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1991272.1"
                     /translation="ATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSA
                     ESEKLYKQLLNSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVG
                     EKMSVGVTAPIVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTH
                     LPLQTVGEAIGEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGL
                     EYDDLNSVLDVTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSG
                     VDSSAALSSLSKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGP
                     RMADAIRRGTLNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEA
                     VQVSLLPFFEKATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSV
                     TKIVGEVKSFIGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNS
                     FFKGVSDVAVEIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWS
                     KAWQGLVDIFGGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSI
                     SKLPYLAQGGHLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIE
                     QHIHIGKVDANNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            2180..2884
                     /locus_tag="IXH60_002384"
     CDS             2180..2884
                     /locus_tag="IXH60_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1991273.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            2881..5640
                     /locus_tag="IXH60_002385"
     CDS             2881..5640
                     /locus_tag="IXH60_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1991274.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            5653..>5882
                     /locus_tag="IXH60_002386"
     CDS             5653..>5882
                     /locus_tag="IXH60_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1991275.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKI"
BASE COUNT         1980 a          912 c         1348 g         1642 t
ORIGIN      
        1 atgcaactca aacagaaaaa ttgcgagcgg ctaaacaaaa attagaaaaa caattaggat
       61 tggctgaaca acgatctcaa atgctgagag aggaatatga aaaatctgtc aaagaaaccg
      121 gtgaatattc tgctgaatca gaaaaacttt ataaacaatt actcaattct gaaaccggag
      181 aaaataaact tcgtacagca ttagaacaga cgaacgatgc acttaaagaa caaggtgacg
      241 tttctgttga tacagcaaaa aaactccaga aaatcgaaga aacaggtgag aaagtaaaag
      301 gcgttggtga aaaaatgtct gttggagtaa ccgcgcctat tgtagcggca ggagcagcag
      361 gacttgcagc atttggtgaa gttgacgagg cacttgatac catcattaca aaaaccggag
      421 caacaggtga tcaagctgat agactttcac agtctttcaa aaacgttggt tcaaatactc
      481 atttaccttt acaaacggtt ggggaagcta ttggtgaggt aaatacacaa tttggattca
      541 tggataaaaa actggaagat tcaaccaatt atctcctaca gtacgctgaa atcaatgata
      601 cagatgtttc gcaatcagca atatctgctc gacaagctat tgatgcttat ggactagaat
      661 atgatgattt gaattctgtc cttgatgtaa caacgaaaac atcgcagaat actggtcaat
      721 ctgtagacga cttgatgcaa aaagcaattg atggcgcacc acaaattaaa caattagggt
      781 tgagctttgg tgaaggggtc actttacttg gacaatttga gcaatctggt gttgattcaa
      841 gcgcagcttt aagtagttta tctaaagcaa cagtggctta tgcgaaagac ggaaaatctt
      901 taagtcaagg actagaagaa ctacaagaca aggtaaaaaa tgcaggttct gaaacagaag
      961 ccatcaatgc agcagcagaa gtattcggga ctaaaggcgg tcctagaatg gccgatgcaa
     1021 ttcgtagagg tactttgaac ctggaagatt tagcaaaaac tgctggagaa agtgggggag
     1081 ctgtaggaga cacattcgat gctactctcg acccaattga tcaagccgat caagctatga
     1141 acaatgcaaa attagcaatg gctgacgtag gtgaagcagt ccaagtaagc cttttgcctt
     1201 tttttgaaaa agcaacttcc ttattacagg aattctctaa atggtggggg tctctagatc
     1261 aagatacaaa gaattggatc ataacaattg ctggtatagc ggcagcagtt ggaccagtac
     1321 taatcgttct tggttcactt atgggatctg ttacaaaaat cgttggggaa gttaaaagtt
     1381 ttataggagt ttggcaagga cttgctggtt tatttggaat gtcgggaggc tggtttgcat
     1441 tagcagttat agctattggc gcattagtta ctggactaat ttgggcatat aataacgtta
     1501 aatggtttca tgatggagtg aattctttct tcaaaggtgt ttcagatgta gctgttgaaa
     1561 tatttaattt tttaagtggt tattttggtg gatttttcga tggtgttata gcaaatttca
     1621 ataattttta taatgctgga aaacgaattt tcagtggttt tctagatttt atcactggag
     1681 tatttacagg gaattggtca aaagcatggc aaggtctagt tgatattttt ggaggtattt
     1741 tcgatggtat tgttgctgta gggaaagctc ctattaatgc catgattgga ttgatcaatg
     1801 gatttattgg tggattgaat aatataaaga taccaaaatg ggttcctggt gtaggtggaa
     1861 aatcattttc tatttcaaaa ctgccttatt tagcacaagg cggtcatcta atcaatggac
     1921 aagcgattgt tggtgaagct ggacctgagt tactgactgc caagaatggt aaaaccacag
     1981 ttactccatt atcagacgaa gaaaaacgca aagggatcag tggaagagtt tctggaggta
     2041 gcattgaaca gcatatacac attggcaaag tcgatgcaaa caatccatct gagttagatc
     2101 gtatgaatcg taaatttgct aaagcaaaca aacaggctat tcgtgatttg ggaggtgttc
     2161 ctatttgagt cgacaattta tgaatccaga tgaaccaaat tttatttgga aaaatcggaa
     2221 tgcagtaatt gatatgaatt gcatcattga ggcagagctt ccagatatta tgccgaacaa
     2281 gcgatacgaa acttacacga ttcagggccg aagcggagaa ttgacggaga cgtttgatga
     2341 ctatgaacct tttgatttgg aaatagaagg gatcactatt ccacattcga aactgagaga
     2401 agtcaaacga tggctcactg gtaaaagcca attaatcaca cataatgatc cagacaagta
     2461 tctagaggct atctgcagca tggataaaga agtccctttc gaaaacgagt ggggcttctt
     2521 ttatacattc gatgttactt ttcgttgtca gccactcaaa agaaaaatcg gtgaagcacc
     2581 taaaattttt cattctagtg ttatggatat ttttgatcat ggtgacgaaa ttgcacatcc
     2641 ctatctggaa atagaatcga acggaggaga tataacgatc gctgtcggtg ataaaagcct
     2701 aacaatactt aatacgttag ctgcaacaat cgtggttgat actcagcttg gaaaagcaat
     2761 acaagaaggt ttgaatttat ttacgcgtgg tgattggcca gtattgcagc ctgaatggaa
     2821 tcaggtaaaa atatccggaa aatttaaaga agtgcggttt tggaatagga gtgtgtatct
     2881 gtgacgcaag aatttattta tgcttataaa aaaatgccag atgatttgag cgtgaacgga
     2941 gcgtcgttag ttgattggga agatttgcca gaaatcaatc gtgtgctgaa tggccaatat
     3001 cgtttttatg gtaactattc aagaagcgga cagtatcgct cttatttaaa aaaaggaaat
     3061 tttataaaag cgaaggtacc agatggatca tggcaatatt ttgaaattta taatgtcaaa
     3121 aagaatttga cctccgtttc tgtaacagct cggcatattg gttttatggc caataagaac
     3181 tttattgtca agtcgtttac ggataacggc aatgggtctc agatcatgac caatcttaaa
     3241 aacagtttgg catttgatca aaaatttaac tatctttcga atgtagggac aacgcaccaa
     3301 tttacagcaa gacaagtcgc gccagttgaa gcaatcattg gttcaaataa cggtaatcaa
     3361 aatttgactg gcgtgactag cgcggaactt gatatggata actatgattt gaaactggta
     3421 aaacagattg gatcagataa tggttttcgg attgattttg gcatcaattt agaagcaatc
     3481 gaagaagaaa ttgatgaaga atcaattgtc aatagtttgt atttggttgg cggtgttcct
     3541 gataacgatt atgatgaaga caaagaacct atcgaatatg gttatttaga aattgatgga
     3601 gtaaccaacg agaatcgacg tattgccaaa cgtgaaaacg gagattgtaa aacagttgat
     3661 gaattgatca aatggggcaa aaccttattt gacaatgatc gtatccatga gccaaaagct
     3721 actcatacag tcagtatggt ggcgttggaa cacacactcg aatacggtga catgtatcaa
     3781 gagctagcgt ctttacactt tggtgatgtc gctcatgtta gggcgaaaga gctagatatc
     3841 gagataaaag agcgtatggt cgaatacact tatttcccaa cgcttgggaa atataaggat
     3901 attgttttag gtaatgattt atcactgtac acttcggcag taaatactca agcacaagaa
     3961 ttgaaaaaga aaatcgacaa tcgaacagaa acgttagttc aaaatgtgtt gaatgcgacg
     4021 gcatggatca ctggtaattc tggtgggcat gtcgtttttc gtccagaaaa agctccatca
     4081 gagattctta tcatggatac tccaaatgtg gcgaatgcaa agcgagtgtg gcgttggaat
     4141 ttaaatggct taggttattc agacaacgga gtaaatggcc cgttcggtat tgctatcaca
     4201 tcaaaaggag aaattgttgc tgacttcatc aaagtaggaa caataaatgc agaagtattc
     4261 gaaacttcct ttaatgctta tggtgatgtg ttaaaacttg taaaaggcac gcttcaaatt
     4321 tggaatgaaa acaaaaaaat catggagcta accaaaaaag gtatggaatt ctggaataga
     4381 aaagaatcca ttggaacgat cggaacaaca gattcagcag gaaatccatt tccaaatgct
     4441 gtaacaccta cacctcttga ggaaaattct ttggtaatac gtacaaatgg cgatggaaaa
     4501 tacattttaa tttctccaac tgcagaaaaa ggatttgtgt tattaggaaa cggaaaagca
     4561 tattatttcg gtgatttaga tattcaggga aaacttactg ttagaggtaa agaagtgata
     4621 ccaggacaaa atggcggtcc atctggcgga ggagaaacac ctggtgggta tcctgatgaa
     4681 ttaaaaacag atgcagaaaa gagagcttgg agaatatacg atattttgtg taataacgga
     4741 tttacgaaac aatctgcatg tggaatattg gggaacattc aacaagaaac aggaggaact
     4801 tttgatcctg atactgttca aataggtgga ccagcatacg gattagttca gtgggatggt
     4861 tcttcatatc ctcttgttgg tccagcaaca tgggatggaa aagtttatgt tcaaaactta
     4921 tttaatgctg caggtattaa agaaccaata acgagcttag atgcacaagt tcgattgctt
     4981 atttggacat ttacaaatgg acaatggatg ggcgtagtac aacctacgac ggttgatgga
     5041 tttaaggcct gtactgatcc aagacaagca gcatatgctt ttgaacgaaa ctatgaacgt
     5101 ccggcagcga cacatcctga acgtcaagat tatgcagtta actggtataa caaatttaaa
     5161 gatttaaaac caggaggagc tactggagaa gcaggactaa aacatttaga gtctttgatt
     5221 ggacaacgaa ttggcaatgg tcagtgttat ggcttgtctg cagaatattc aggatatctg
     5281 ggtggttgtg gcatgggtgc tggaacaaaa tatggtttaa ctcatgtgat aggaaatact
     5341 tctgcagcat ctgatattgg tattgcctat gattggtctg ctgttggttg gaaagtgatt
     5401 caaaacccta gatatgatca attagtagtt ggtgcaatta ttaattgggc aagaggtgga
     5461 caagtgggtt catggtttgc agacggaact tatggacata ctggcgttat ccgaggctta
     5521 gctaatggtc gtatgcaaac ttatgaacaa aacacagagt taggtatgat ttgtggaaag
     5581 ttagatcgcc agtattatag tgcaagtgca atttcttcca ttgtcatacc accaaaatag
     5641 ggaggtgatt taatggcaaa gtggaatgtc atattaagta caacagaacc ctataattac
     5701 gtggggatga ttcaagttcg acaaggcaat aaaaacactg aagttatgga agctacaatt
     5761 gtcgaaaatg gtcttccata tgatttatca gaatgtaagg tatattttga atcagttgtt
     5821 ggcggaaaat atccagtcca attagaaaca agaattgtgg atgctaaaaa agggaaaatc
     5881 aa
//
LOCUS       DACEZN010000154         5876 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEZN010000154 DACEZN010000000
VERSION     DACEZN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.154"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            640..1374
                     /locus_tag="IXH60_002387"
     CDS             640..1374
                     /locus_tag="IXH60_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1991276.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1390..2559
                     /locus_tag="IXH60_002388"
     CDS             1390..2559
                     /locus_tag="IXH60_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1991277.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2575..3570
                     /gene="lacD"
                     /locus_tag="IXH60_002389"
     CDS             2575..3570
                     /gene="lacD"
                     /locus_tag="IXH60_002389"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1991278.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYAEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3649..4575
                     /locus_tag="IXH60_002390"
     CDS             3649..4575
                     /locus_tag="IXH60_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1991279.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4594..5844
                     /locus_tag="IXH60_002391"
     CDS             4594..5844
                     /locus_tag="IXH60_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1991280.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1966 a          957 c         1202 g         1751 t
ORIGIN      
        1 tttgaaaaat gctatttcga ggtagaagga tattatataa gcacggttga ctaaacgaag
       61 tgataataaa aaaagttatt caagaataag aaaaattatg atacgaattc agataaactg
      121 gagaatacag agacctttaa aagggataag aaacaacata aattcctctt aagagagaac
      181 gaaaggacaa agccagcaac ttgatacacg agctagtatt agaggaattt attctcatag
      241 actccctttt gaatacgttt caactttaat tttcatgttc ataaatgatt tgaaaccaaa
      301 gcaaatgcgt tgcacttttc ctccgtcttc cgaaaatcgt tcaattcact ggtaagtagt
      361 catttgatag tttgaggcaa attgttgatt gatgaagggt aggagctaaa aaaagatacc
      421 agttgaagag agaaaaagaa aaaaatagag ggtgttcttg aatgaaaaac gttggaaaat
      481 tatttttcat ttccttttat tcctattaaa gtcagtgatg tcaatcatat taacaagact
      541 aaaaatgaaa ataaaaaaaa gattgttctt agtattgact cataaaaaat accactacta
      601 tacttacatt gtggttatag ccagttagga gagacatcga tgcaaaatac ctttcaatca
      661 aaatcacttt attatcagct ggtagaaata ttgaaacaac gaatcgaaaa cagtatggtc
      721 gctcatgaca agctgccatc agaaagagag ttgaccagtt tttatggcgt tagtcgcacg
      781 acggtgcgac tagcattgca agaactggaa aaaatgggct acatttatcg acaacatgga
      841 aaaggaacct ttgtttcgga attaaggaca aatgcgactg atctagctgg cgcatatagt
      901 tttaccgaac aaatgcgggc aatgggcaaa catcctgaaa ccaagatatt ggccttcaag
      961 aagattgaag caactaaata tttagcgagt catctgaatg ttcaactagg tgagccttta
     1021 tttcaattag accgattgcg cttagctgat gatttgccaa tgatgatcga gaaatcctat
     1081 ttgccagtga agctatttat gtccctttct caagaaattc tatttctgaa acctttatat
     1141 aatttgtttt cagagaattt caatcaaatc gtacggttgg ctgacgagga attttatgcc
     1201 agcattgtcc agaaagaaga tagtgagttg ctaaatattc cagaaggatc accagtgctc
     1261 catttagttc gctcaaccta taatctcgag aacgaagtga ttgaatttac attaagtgta
     1321 gcgcgggctg ataaatttca ttataagatc aggcatctgc gttccactaa ctgaattgga
     1381 ggaaagaaaa tgtttatgtt ctcaaaagaa gaactacgaa agctaggagc agaaaatact
     1441 acccgagaaa ttcgacaaca accggaactg tggcatgaga cgtttgaccg ttatcaagaa
     1501 acagttgcag atttagatcg tttgtttacg gatgcgaaac aacatgctgg agagcgacca
     1561 atccgagtca tttttacagg ggccggaacc tcgcaatatg tgggagatac actaattccg
     1621 tttcttacta gtcaaggaaa tacgaaccag tttatttttc aaagtattgg ttcgaccgat
     1681 atcgttgcta cgccaaaaga atacctgatt cctgatgaga caacgatctt ggtctcattt
     1741 gcccgtagtg gaaatagtcc agaaagtcta gccgcagtta agatggccaa tcagctggtc
     1801 aacaagatct atcatatcgt gatcacctgt gcaccagacg gattattggc tacacaaatg
     1861 cctacaaaaa aagaaaatct agttttacta atgccaaaca aagccaatga cgctggtttt
     1921 gctatgacaa gcagtttcag ctgtatgacg ctgactgcgc tacttatttt tgatcaagca
     1981 agcaatcagc aaaaagaaag atatgttcat caattaagcc gactaggaga agaagtcatc
     2041 caacgagaat cagagatcca agggtatctg gcatcagact ttcatcgcat catttactta
     2101 ggttcgggaa gcttaggcgg attgactaga gaagcgcaat taaaagttct cgagttaact
     2161 gctggaaaaa tcgcaaccgt atttgataca tctttaggat tcagacatgg tcccaaatct
     2221 tttgtcaatg agcaaacatt ggttttcctg tttgtcagca atgactcata tacgcagaaa
     2281 tacgatgtag acatgctgga agaaatcaaa aatgacggta ttgcccaacg cgttgttgga
     2341 attggtcaaa agggttcatt gtttagtggt gattcctttg aattagttgc agattcgtta
     2401 ctgccggatg gctatcttgc tttagcagat atcatggtag cccaaacgat cgcattactt
     2461 tgttcggtga aggttggtaa tacaccagat acaccatcgc caagtggaac agtcaatcgt
     2521 gtggtcaaag gcgtaacgat ttatccatat gaaaaatagg aaagagtgtg aagcatgtta
     2581 gaatggtcgg aaaataaaag acaagcaatg gaacgtctat cgacgccaga aggcattatt
     2641 accgctttgg cgatcgatca gcgaggcgcg ctgaagaaaa tgatgtcggc tttagggatg
     2701 gaagcgacaa aggaaaaaat tgagcagttt aaaacactaa tttcccaaga attgactcct
     2761 tatgcctcag caattttatt ggatcctgaa tacggactgc ctgctgcaca gaagcgtgca
     2821 gcagcgagcg gtctgctgtt ggcatatgaa aaaactggtt atgatcaaac cgtacccggc
     2881 cgcttgccgg atctgctgtc ggactgttca gttctgcgat tgaaagagtt aggtgcggat
     2941 gcggtgaaat ttttactgta ttacgattat gcagaagcag aggaaatcaa tcggcaaaag
     3001 caagtttttg ttgaacgaat tggaagcgaa tgcatggctg aaggactgcc attttattta
     3061 gagttattgt catatgatgc taaaaatgta gatacgaaat cgctttccta tgccaaagca
     3121 aaaccgcgca aagtgatcga aatgatgaaa gaattctcga aaacccagta taaagtagat
     3181 gtcttaaagg ttgaagtacc agtaaacatg tcatttgttg aaggaaatgc aacaggggag
     3241 atcgcctata cacgtcaaga agctgccgct tattttaaag aacaaagcga tgcaacagat
     3301 ttgccgtata tcttcttaag tgctggggta tcagcggcat tattcatgga gacattgcgt
     3361 tttgccaaac atgctggggc acgattcaac ggtgtactct gtggcagagc aacttggaaa
     3421 gatgcagtcg ctcctttcgt tgaaaaagga gaagcggcaa ctttagaatg gctgacagaa
     3481 caagggacac aaaacattcg tcaattgaat gaagtgataa gagaaactgc catcccatgg
     3541 tatgagaaag taagtgaaag cacttgttaa tgtttctaat atattagtag tgataagaga
     3601 tatgtgtgat tataatttat cattattgaa actgggagga tattttatat gaataaaaga
     3661 ttgttagatt attcagcttc agattttttg aaagctaagc cgatggaatt aaagcaggca
     3721 attttggctt ctgaaggtag aacaattatg gcagaaaatg tgccaagtag ctgtccttac
     3781 atgggaaaat cagtgacaaa tgcagaattg gagagagctg ctggagctga tttaattttg
     3841 tttaatgcac tagatctttt taatcctcaa atttcaggtg tacctttaac tctaaaagag
     3901 aatccaataa tctggattaa aaaagcttta ggaagagcta ttggtgtaaa tttagaacct
     3961 gtagacaaag aagttttgat gagtgaaaat cgaactgaaa tttcaagtgg tagaatcgtt
     4021 tctcctgaga ctttaaaaaa ggcaaatgaa ttaggcttta attttatttg tttaactgga
     4081 aatccaggaa caggagtatc taatagaact attgagaaag caataacatt atccaaatca
     4141 atttttaatg gattaattat cgctggaaaa atgcatggtg ctggtgttga tgaaccaatt
     4201 atgaacttag atatagcaaa aagatttatt aaaacaggtg tagatatttt attagttcct
     4261 gctccataca ctgttccgta tttcatggaa agtgatttgc gaggaatcgt tgattatata
     4321 aaagatttta atagaaataa atctattgag gaaaaagttc tagtactaac ggctaatgga
     4381 acaagccaag actcttctga ccaagaaact attaaaaaaa ttgcattagc atcaaaagca
     4441 tgtggagctg atattcaaca cataggggat tctttaaatg gtatttgtct tccagaaaat
     4501 atttttgctt tgggtcaagc aattcgggga tatcgtcatc aaattattat gttaggaaaa
     4561 tctaattatc gttaattaag gaggaaatga aagatgatcg acaaagttca aaaaatagct
     4621 gcaaaaatta gtaatcaaag acatctacta gctttaagag acagcatggc tgcagttatg
     4681 ccattaatca ttattggttc cttttttatg ttattagcaa atttcccgat agaaggattt
     4741 acaaaatggt tatctgaaat tggagtacaa tcctttttga acaaagcatc agattcaaca
     4801 tttggtatta ttggattagc tgttacctac tctttgggac acaatctagc aaagacttat
     4861 ggtgtagact cattatcagc aggattatta tctttgagtg gatatgtttt acttacacct
     4921 ttattggatg cagatggttc tataggattt ccaacaaaat actttggtac ctcgggtctt
     4981 tttgttggta ttattgtagc acttgtatct acagaaattt tccggtggtt tgtacttaaa
     5041 aatattacaa ttaaaatgcc ggcaagcgtt ccaccaaatg taagccgagc tttcgttgct
     5101 attattcctg gcttttttgt cgttctacta tggtttattg ttgttgtaat ttgttataaa
     5161 cttggaattg aaaatgttca tgctcttatt gcagatacac tcgcaaaacc actatcttta
     5221 ctgacgaaaa ctttaccagg aattatattg gttatactta ttcaatgttt cttttggatg
     5281 tttggtattc atggtgcaca ggtaacgggg ccaataattg aaccgttatt attgcaaaac
     5341 tctgatgtaa atagaattgc ttatcaagct ggaaaagaac ttcctaacat tataacttat
     5401 gaattcttat ataactttgt tttttctggt ggtgcaggtt gtttatttgc acttgccatt
     5461 ttaacatttt tcttttcaaa gagtcaggaa aataaaactt taggtaagtt atcaatcgct
     5521 ccagtaagtt ttcaagtagc agagcccctt ctatttgggt ttccaactat attaaatata
     5581 aaaatgttaa ttccatttgt aactgcacca gtagtaacaa cattaattac ttatttttca
     5641 atgagtatgg gattagttgc aaaacctgta ggagcaacaa tcccatggac aactccaccg
     5701 ataattgccg gatttttagc ttctggtgga cgaataagtg gtgcagtgat tcaagtaata
     5761 acaattgcaa taaatgtttt gatatactat ccattcttta aattagatga taatgccaag
     5821 ctaaaaagcg aaaaaaatga ttagaactac ctatatgttt ctaactgtag ttaagt
//
LOCUS       DACEZN010000155         5846 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEZN010000155 DACEZN010000000
VERSION     DACEZN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.155"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            127..579
                     /locus_tag="IXH60_002392"
     CDS             127..579
                     /locus_tag="IXH60_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991281.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IXH60_002393"
     CDS             593..1282
                     /locus_tag="IXH60_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991282.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IXH60_002394"
     CDS             1407..3758
                     /locus_tag="IXH60_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1991283.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IXH60_002395"
     CDS             3838..4233
                     /locus_tag="IXH60_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991284.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IXH60_002396"
     CDS             4558..5175
                     /locus_tag="IXH60_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1991285.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IXH60_002397"
     CDS             5216..5515
                     /locus_tag="IXH60_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991286.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(5704..>5846)
                     /locus_tag="IXH60_002398"
     CDS             complement(5704..>5846)
                     /locus_tag="IXH60_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1991287.1"
                     /translation="QSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGI
                     TA"
BASE COUNT         2023 a          793 c         1183 g         1847 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagg gttctgttgc gaagtttgaa aatcaaacgc gccctctctg aactccaaat
     5701 atcttaggct gttattccca ttaatacctt gatttcagta gacaccgaaa agccgaagag
     5761 cgttccattt cttcggttct ttttatatat tcctcgaatg gtctccatgc ccttaatcgt
     5821 ggaagaggct gtacggagac tttgat
//
LOCUS       DACEZN010000156         5830 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEZN010000156 DACEZN010000000
VERSION     DACEZN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.156"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            137..1456
                     /locus_tag="IXH60_002399"
     CDS             137..1456
                     /locus_tag="IXH60_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ1991288.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1897..3537
                     /locus_tag="IXH60_002400"
     CDS             1897..3537
                     /locus_tag="IXH60_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1991289.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3691..5013
                     /locus_tag="IXH60_002401"
     CDS             3691..5013
                     /locus_tag="IXH60_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1991290.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5075..5506)
                     /locus_tag="IXH60_002402"
     CDS             complement(5075..5506)
                     /locus_tag="IXH60_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991291.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5570..>5830)
                     /locus_tag="IXH60_002403"
     CDS             complement(5570..>5830)
                     /locus_tag="IXH60_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1991292.1"
                     /translation="VTIALTSSFMRFDLSMLFYVIPAAVIGGLWGARFSRVLSPKKVT
                     FIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSFFK"
BASE COUNT         1796 a         1129 c         1095 g         1810 t
ORIGIN      
        1 taattaaaac catgtgaaac tgcctcacgg cagcttacaa ttactttttc atcttcaccc
       61 gttaagtgat gaaaaaagaa cccctttctg ctatggttaa agtgactaaa ccaaccaaaa
      121 gaaaggagtt cttttcatga ctagcttaca caaaaaccaa gtaaaattca attcaaatat
      181 cactatttct catacaggtg gtcaattatc gagtgattcg ggtctcgtcc tagtgaaaga
      241 gctgatgaac atctttggtt tttctgaact ggctaagcaa cacattcaca ttgaagacga
      301 acgagcgtat tttactcatg acaacttatc catacttgag cagttcatta tgcaattaat
      361 tgctggttac tcagctgatt ctgcagctaa tctcctgaga caagatcctg tgtttaaagc
      421 tgttttagat aggaaagaac tcgcttctca atcttcactt tcccgatttt tagatcgact
      481 atctgaggag aatatccatg aacttcaagc tttgaaccaa gaacttatcg ataaagcacg
      541 cctcatccgt aatgatacgg aattaatcat tgatttagat tcgacccatt cagatacatt
      601 tggtcaccaa gaacaaacga attataatac acactaccaa acctatggtt accatccatt
      661 ggtagctttt gacggattga ctggtgactt cttaaaagct gaactacgtt caggaaatca
      721 atatacatca aaaggcgtaa aggagtttct cacaccttta ttagagcact ataatcactc
      781 tctaccaaac actgacatct tggttcgtgg agacagcggg ttcgctacac ctggtgtgta
      841 tgattcgtgt gaatcaaaaa agagtcatta tgttattcga ctgaagaata atcgtagact
      901 aggtcaaata gctgagaaat cagtacttta tggcgataat caaaagtggg aagaacgaga
      961 agttcagtac ttctcgacca cttatcaagc acaatcctgg tcacaaagtc gtcgcgtgtg
     1021 tatacgctca acacgtgaag cgggcgaact actctttcga catgaattta tcgtgacgaa
     1081 tctatcagaa aatgtttctc ctgataccat cttttctatc tatgccaaac gtggcacaat
     1141 ggagaacttc attaaagaag cgaaagctgg cttttacttt gacaaaacag acagtcctcg
     1201 ctttttggag aatcatgtcc gaatgatgct cagcttgata gcttacaact tagtcaactt
     1261 cttaagaacg attggctttg aagaagtcca aaagggaatg accattcatt ctattcgatt
     1321 gaagtttctg aaagttgctg ggaaattagt ccaaacgggt agacgagtct atctcaaatt
     1381 atctagttat catgtgtatc agaatgaatt ctacagggtc tttgctcgcc tgaggcgagc
     1441 cagtcaatgg atctaatcca acaatctaac gattttttta ctgggaagtt atccagggag
     1501 aagtatgctc aaaatgcctt acacccaaaa tttattcctc aatatttttg aacagtgaat
     1561 gtttgaagta actttttagt caaaatcagt tgatagggat attagtgata catatttcaa
     1621 taaaatgtgc caaagaatta aagctatgaa ttattcagga ttctatttat ctatctaaca
     1681 tatcctttat tcttctactt ttctttcttt agcatataag agatacgcat agtaatcaag
     1741 caaggattgt tgatataaaa agaacaaata ccgctatttt gatttctttt gactctatta
     1801 ctcatataaa taaaatttat tatacctgct ctttttgact atatcctcat ctgactccta
     1861 tacttcgttt gtattaaaac ttaggaggaa tcattcatgc ttacagggct aaacttatta
     1921 aataatcctt ttctaaataa aggaacagct tttacaaaag aagaacgggc aaaatacgga
     1981 atcactggta tgcttccaag tactgtccaa acattggaac aacaatctgt ccaagcttac
     2041 ggtcaatact taagcaaaca gaccgatttg gaaaaacgta tcttcttaat gaatctattc
     2101 aatacgaacc ggacattgtt ttataaattg atgggcgaac atttagttga attcatgcct
     2161 gtcgtttatg atcctgtcgt tgcggattca atcgaacaat acaacgaaat tttcttgaaa
     2221 ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa atatcaaagc aagtttgaaa
     2281 aacgcagcag atggacgcga cattcgtttg atcgttgtga cagatgcaga aggaatctta
     2341 ggtatgggtg actggggtgt gaacggtgtt gacatcgcta tcggtaaatt gatggtctac
     2401 actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg tatcaatcga cgctggtact
     2461 aataacgaag aattattgaa taatccatta tacttaggga atcgtcatgc tcgtgtagaa
     2521 ggcgaaactt actacgaatt cattgatcaa ttcgttcaat cagctactga attattccca
     2581 gaattgttat tacattggga agattttgga cgaggaaatg ctgcaacgat cttagaaaaa
     2641 tacgaagata aaatcactac tttcaatgat gatatccaag gcacaggaat cgttgtacta
     2701 gctggtgtat taggtggatt gaatatatca ggtgagcaat tgaaagatca aacgatcttg
     2761 acatttggtg ctggtactgc tggtgtaggt atcgcaaaca tccttttaga tgaaatgatc
     2821 cgtcaaggtg tacctgaaga agaagcacgc caacatttct atcaagtaga taaacagggt
     2881 ttattgtttg aagatacaaa aggattgact cctggtcaaa tcccattcgc tcgtaaacgc
     2941 tcagaattca ctaatgctga tgaattgaca aacttagaag cagttgtaaa agcaatccat
     3001 ccaacgatca tgatcggtac ttctacccaa cctggagcat tcactgaaga aatcgtgaaa
     3061 gaaatggcgg ctcatacccc acgtccaatc atcatgcctt tgtctaatcc aacaaaattg
     3121 gcagaagcaa aagcaaaaga tttgattgaa tggacggacg gaaaagcttt agttggaaca
     3181 ggtatccctg ctgctgatgt ggaatataac ggtgtaactt accaaatcgg tcaagcaaat
     3241 aatgcgttga tgtacccagg tttagggctt ggtttgattg cttcaacagc tacacgagta
     3301 aatgctgaaa tcatttctca agctagtcga gcattaggcg gaatcgttga tgtaacaaaa
     3361 ccaggagcag ctatcttgcc tccagtagca aaaatcactg aattctctca aacgattgct
     3421 gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc gtgaagaaat cacggatatc
     3481 aaagaagctg ttgaatctgc taaatgggtt cctgaatata aatctttaga agattaagat
     3541 tgttatagta aaagaacata cagaaacttt gtatgacgtc taaaaagaaa aaagcgactg
     3601 cggcaaaaca gcagccagcc aactactgct cttttgccgc ttctctttgg cttttttcat
     3661 acgatacaga ataagaaagg atctgtaaaa atggcacaaa aaacaacaac cgcaactgca
     3721 gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa ttaccggcgt aagtttgccc
     3781 ctttatttga tcatggtcct tgtcctgatc attgcaatgg ctttaggaaa attaccgacg
     3841 aatatgattg gcccaatcgc tgttttggtc attttcggta atttatttca ttttatcgga
     3901 acaaaaatcc ctatagtcaa aagttattta ggtggtggct ccgtttttgc gatctttgcc
     3961 tctgccgcga ttgctacatt tgggatctta cctgagtatg tagtaaaatc cacagaaaac
     4021 tttgtcagca atatgggctt tttagacttt tatattgctg ccttgattac tggtagcatc
     4081 cttggtatga accgtaatct attgatgaaa gcttctgttc gttttattcc cgttgcactt
     4141 atttcaatgg tcgtctcctt ttttgccgta ggactcgtag gtatgcttat cggaaatggt
     4201 tttgcaaatt ccgttttata tatctcattg cccgcaatgg ctggtggtgt tggagccgga
     4261 gctgtacctt tatcaacgat ttatgcaaat gttttaggta cggacgcttc ttctatcatt
     4321 tcccgcctga ttcctgcgac agcaatgaca aatgtcctag ctatcattgg tgcagcgtta
     4381 gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta atggtaaatt aatggaaaaa
     4441 ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt ccattccaca attaggtgtc
     4501 ggtttgatgg tatcattgac tttcttcatc ttaggacaaa tctgtaacta ttttgttcct
     4561 aaagtccatg cttacgcatt catgatcatt atcgttgtta tctgtaaaat cgcaaaccta
     4621 ttaccagaat attacgaaga tgcagctatc atgttcaaca acttgattgt caaaaacttg
     4681 acagcagctg ttcttgccgg tatcggtatt gcgttattga atctaaatgt attggcttct
     4741 gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg ttatcgcgat ttcagtagtt
     4801 tcaggatttg tcggtcgttt gtttggttta tatccaatcg aatcaactat tactgctggc
     4861 ttatgcaaca actcgatggg tggtacagga aatgtagctg tcttatctgc agctaatcgg
     4921 atggaactta ttgcttttgc tcaaatgggc aatcggcttg gtggtgccat cgttttgatc
     4981 atctcaggtt tcttgatgca attactttct tagtcaaaaa aaatacctga tcatcaagta
     5041 tcggcttgat gaccaggtat tttttgataa aaatttacag gattttcaaa aatctttttt
     5101 gcattttatc ggaatgataa ccgatgcgtt cataaaatcg gtgtgcttct actctgtatt
     5161 ctgcagaatt aagtcttatt gctgatattc ctcgtctttt cgcttcttct acgccagtca
     5221 tcaaggcctt tccgattcct cttttttcag catgtttaga cacagcaagt gccataatat
     5281 tgaacatcgg atcgctgaaa atcgattcat aggactccgc ttgtacatag ccagctgctt
     5341 ttttagaagt aggatcttca tagaccaaga aaaaattttt ttctggctcc ttcaacagtt
     5401 tcttcatttg tctcaccgta acggttaatg gtacatcata tcctaattgt tccgcattga
     5461 tttctcgtag tgcttctgca tctcctaaac gcatttcccg caacatccaa atcaccagcc
     5521 aatttttatt tttttaaatg aattttttta tcacagtata ctcctttttt cacttaaaaa
     5581 aagacttttc agtgattttg acttgcttga atccagatcc ttctaatgtt tgcaagatga
     5641 cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac aatcgcttga aaaataaagg
     5701 tcactttttt tggagacaaa accctgctaa atcttgctcc ccacaaacct ccaataacag
     5761 cagcggggat aacataaaac aacatagata aatcaaagcg cataaagcta cttgttaaag
     5821 caatcgttac
//
LOCUS       DACEZN010000157         5805 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEZN010000157 DACEZN010000000
VERSION     DACEZN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.157"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            97..1101
                     /locus_tag="IXH60_002404"
     CDS             97..1101
                     /locus_tag="IXH60_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1991293.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1102..2046
                     /locus_tag="IXH60_002405"
     CDS             1102..2046
                     /locus_tag="IXH60_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1991294.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2046..3008
                     /locus_tag="IXH60_002406"
     CDS             2046..3008
                     /locus_tag="IXH60_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1991295.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3035..3949
                     /locus_tag="IXH60_002407"
     CDS             3035..3949
                     /locus_tag="IXH60_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1991296.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3975..5756
                     /locus_tag="IXH60_002408"
     CDS             3975..5756
                     /locus_tag="IXH60_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1991297.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
BASE COUNT         1873 a         1077 c         1270 g         1585 t
ORIGIN      
        1 gataagaata acctttgtta taaaacgatt tattctgtgt aatgtccttt tttattaaga
       61 aaaacttcca tttaggaagc agagaggggt attcgattgt ccgataacca attattagaa
      121 gttcagaacc tacatacagg attccgactc aaagatgact attacgatgc agtagatgac
      181 gtttcattta ctttagacaa aaacgagatt ttagcaatcg ttggggaatc tgggtgcggg
      241 aaaagtacct tggctactac gattatggga ttacttgatc ctaataacac aaaaattaca
      301 ggtgagattt tatataacga tctgaattta atcgacctga acgaaacgct ctataataaa
      361 atccgcggaa atgatatcgg aatgattttc caagatccgt tgtctgcatt aaatcctctc
      421 atgagaatcg aagaccagat caaagaagga ctttcctatc acacaaagat gaatgcagaa
      481 cagcgtcaag ctcgtgcact agaattgcta gaacaagtag ggattcctaa tcctgcgaga
      541 gttggacgcc aatatcccca tgaactatca ggcggtatga gacaacgtgt catcatcgca
      601 atcgcaatcg cctgcaaacc tccaatcgtc attgcagatg aaccgacgac agccctagat
      661 gtaaccatcc aagcacagat tcttgattta ctgaaagacc ttcaggaaga aacaaaaaca
      721 gggatcatct tgatcactca tgacttagga gtcgtagcag aaatggctga tcgagtggca
      781 gtgatgtatg gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt tgatcatcct
      841 aagcatcctt atacacgctc tttactgaac tctataccac aagaaggaaa tcatgatgca
      901 gaactccatg tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg taaaggttgt
      961 cggtttgcac cacgtattcc atggatacca gcaagcgatc atgaagaaga gcctatcctt
     1021 catgaagtcg caccgaacca cttggtacgt tgctcctgct ataagcattt ccactttaga
     1081 gacgaaaaag gagaggtgta gatgacagaa ttaatcacaa tcaaagacct gaaagttcat
     1141 taccctattc gcagtggatt cttcaatcga gtaacggatc atgtatatgc tgtagatggt
     1201 gtggatttta ttatcgaaaa aggaaagaca tatggtttag tgggagagtc tggctctgga
     1261 aaatctacta cgggaaaagc cgttgtggga ttagaaaagg taacagccgg acaaatcatg
     1321 tatgaaggaa aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta taacaaggat
     1381 gtgcagatga tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg agtgttggat
     1441 atcattgctg aacccatccg aaattttgaa cggttaagcg atcaagaaga aaagaaaaag
     1501 gtaaaaggac tgctagatat cgttgggatg ccggaagatg cgctatataa atatcctcat
     1561 gaattttcag gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc taccaatcca
     1621 aaactgatcg tagccgatga acctgtatct gctctggatc tatctgttca agcgcaagta
     1681 ttgaatttca tgaaacgaat ccagcaagaa tacggcctca gctatttatt catttcacat
     1741 gatttaggtg tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa aggtcgattt
     1801 gtagaaatcg gtacaagaga agacatttat aatgacccgc gacatatcta tacgaaacgt
     1861 ctgctttcag ctattccgca aatcgatgta gaaaataggg aaagccacaa agaaaatcga
     1921 cgaagagtag aacaagagta tatccaaaac caaaaagaat actatgatgc gactggtcga
     1981 gtctacgatt tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga tggaggtgca
     2041 agctaatgtg gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa gttattattt
     2101 taagtgttct gatcttctta ttagcccaag cgatgccggg ggatccattt accggtttga
     2161 tcaatccaaa tcaggatccg gcagtgatcg aacagatgcg acaagcagct ggcttgaatg
     2221 acccatggta tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc gattttggtc
     2281 aaagtttctt atacaaacgc tctgttgctg acttgatcgg cgaacggatc atcaacactt
     2341 tatatctatc gatcttgaca gttgtcctta cttatttgat tgctgtgcct ctggggatgc
     2401 ttgctggccg gtatcaaaat tcaatcttgg ataagattgt cgtaatctac aacttcttta
     2461 gttttgctgt tcctttattt atcttcggct tgatcatgct atttgttttt ggttatcgtt
     2521 taggctggtt cccaacaagt ggatcacaaa cactaggttt gcaaggtgga tttatcactc
     2581 aatggataga ccgtttgaaa tatattctgt taccatcaat tacccaagca tttttggcga
     2641 ctgccgtcac aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg ctcgattttg
     2701 tcagaacagc ccgttccaaa ggggtaccga cgaataaagt attcagtcga catattttcc
     2761 gaaatgcagc gttgccgatg gcttctcaaa tggggtatga aatcacttcg ttgattgctg
     2821 gttctgtagt tatcgaaaag atttttggtt atccgggagt cggaaaacta tttattgatt
     2881 ccatcggcca acgggactat accgtgatca catccttagt attgatttta ggaatcgcga
     2941 cgctagtggg gacattactt tcagatatca tcatgagtat cgttgacccg cgtattcgga
     3001 ttcaataaaa ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca atacagcagt
     3061 tcaagaaagt attcctccta tggggatacg aatgattgct cgagaattta aaaaagataa
     3121 aattgctatt ttttcattag gtttgctagt ggttttatta ttagtgatct ttatcggtgc
     3181 gatgctgacc gatcaagaca aagtgatgta tgttagtatc ttcgacaaat acgctgcacc
     3241 aggaacagtt tcgacacaag gacaaaagtt cattctggga gcagatgaag gcggacgtga
     3301 tgtattagga caattgatca taggtgctag aaactctgtt acgatcggat ttgcgatcac
     3361 gatccttact tcgatcatcg gggttggatt agggatcatc tctggctact acggagggat
     3421 cattgacaac atcttgatgc ggatcgttga ttttatcatg attctaccga tcatgctgat
     3481 cattatcgtc ttcgtatcga ttatttccag atacaatgtc tggtccttta tattcatcat
     3541 gagtgcattt tattgggtag ctaaagcccg attattccga agcaagacat tatctgaagc
     3601 aagaagagat tatgtcagtg cctcaaaaac aatgggaacc aatgatttta aaatcatgtt
     3661 ccgcgaaatc atgccgaatc taagttcatt aatcattact aatttgacga tgaactttgc
     3721 ggcaaacatc ggtatcgaga caactttgac tttcttaggt ttcggcttgc cggcaaatgt
     3781 acctagttta ggtacattga tcggttatgc aagtaacgga gaagtcttag tgaatcggca
     3841 atggatctgg ttacccgcat caatccttat tttagttttg atgttgagta tcaattatgt
     3901 tggacaagca ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt atagaagcga
     3961 agggggaaat aggaatgaag aaaacgattt ttggatttgc agcgctgatg tctgctgtcg
     4021 ttcttgcagc atgcgggggc ggaggtacag atacctctgg aaacaactct ggaggcaacg
     4081 gcaataacaa agaggaatta acttttccaa ttgcaacaaa aaataatgat gatgcaatcg
     4141 aagacggcga attagaagct gcggtcgcaa cggacagtca gtttaaagga ttgttccagt
     4201 gggagtttta ccaagattca tatgatgcag cttttatggc tccttctcat gaaccgctat
     4261 ttgcaaacga tgacgatttc agaatcacag acgacggagc tgcaacgctt gatctagatg
     4321 aagatacgaa aaaagcgaca atcaaagtaa aagacaacgt caaatggtca gatggtgaag
     4381 atgtaacggc ggacgatgtg attttccctt acgaaatcat tgggaataaa gattacactg
     4441 gtatccgtta tgacgacact ttccgtaaca tcgtaggtat ggaagaatat catgatggaa
     4501 gtgccgacac aatctccgga atcaaaaaaa tagacgacaa aacagttgaa atcgaatata
     4561 aagaagtaaa cccaagcatg cttcaatcgg gtggtggtat ctggtcatat gcagcaccaa
     4621 aacatacgtt agaaggtgta gcaatcaaag atatggaatc aagcgatcaa gtacgtaaaa
     4681 atccagtgac ttttggccct tattacatga gtaatatcgt tgcaggagaa tcagtagaat
     4741 acttgccaaa cgagcactac tggaatggta cgcctaaatt gaaaaagatc acgatgaaat
     4801 ctgtaccgat ggcaagtgca actgaagcat tgaactctaa attatatgat atgattttcc
     4861 aaatgccaac agatacttac gatacctata aagaaattgc tggctataca aacttaggcc
     4921 gtgaacaaac ttcttatact tatctaggat tcaagttagg taaatggaat gcagatacaa
     4981 agagtgtgga atacaatcca gattcaaaaa tggctgataa atcattacgt caagcaatgg
     5041 gctatgcatt agataataat gcagtaggtg aacgtttcta tgcaggtttg agaagcaatg
     5101 cgacttcatt gattcctcca gtattccagt cataccatga tgatgagatg aaaggattca
     5161 cacaagatct agacaaagcg aaaaaactat tagatgatgc tggctacaaa gatgtggatg
     5221 acgatggttt aagagaagac aaagatggaa ataaactaac aatcaacttt gcttcgatgt
     5281 ccggtgggga aacagcgcaa ccattggcag attactatat ccaacaatgg aaagaaattg
     5341 gtttggatgt ccaatacaca acaggtcgtc tgatcgaatt caattctttc tatgataaat
     5401 tagaaaacga tgatcctgaa attgatgttt accaagctgc ttggaataca ggaactgatc
     5461 caaatccaag tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt tatgaatcag
     5521 aagaaaatac aaaattgatc aacgatatca gttcaagcaa agcatttgat gaagagtacc
     5581 aaaaagaagc atatcaaaaa tggcaacaat atgcatttga agaagcattt gcgattccaa
     5641 cgttgttccg taatgaagta ttgccagtca acgaccgtgt caaagactgg gattggtctt
     5701 atgatgtacc aaacccatgg gcaaatgttt ctgttacagc agattcacgt tcataatcac
     5761 taaaaatgac cgaaaaaggg cgatgtcctt tttcggtttt ttgat
//
LOCUS       DACEZN010000158         5729 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEZN010000158 DACEZN010000000
VERSION     DACEZN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.158"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(35..256)
                     /locus_tag="IXH60_002409"
     CDS             complement(35..256)
                     /locus_tag="IXH60_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991298.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(253..543)
                     /locus_tag="IXH60_002410"
     CDS             complement(253..543)
                     /locus_tag="IXH60_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991299.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(540..1724)
                     /locus_tag="IXH60_002411"
     CDS             complement(540..1724)
                     /locus_tag="IXH60_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1991300.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1906..3309)
                     /locus_tag="IXH60_002412"
     CDS             complement(1906..3309)
                     /locus_tag="IXH60_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1991301.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3331..4104)
                     /locus_tag="IXH60_002413"
     CDS             complement(3331..4104)
                     /locus_tag="IXH60_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991302.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4114..4491)
                     /locus_tag="IXH60_002414"
     CDS             complement(4114..4491)
                     /locus_tag="IXH60_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1991303.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4512..4826)
                     /locus_tag="IXH60_002415"
     CDS             complement(4512..4826)
                     /locus_tag="IXH60_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1991304.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5026..>5729)
                     /locus_tag="IXH60_002416"
     CDS             complement(5026..>5729)
                     /locus_tag="IXH60_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1991305.1"
                     /translation="CNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRRKLIANDI
                     LIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEK
                     NYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTV
                     IAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLL
                     YPTQLLDKIEFDYNI"
BASE COUNT         1707 a         1121 c          955 g         1946 t
ORIGIN      
        1 tatatacttc ctattttatc atgaaaaata tctgttaagc accaatgatt cggttaaata
       61 agtttaataa cacatctttc acgccagctg cattgaacac aagtccgacg gcgattagag
      121 caataatcaa gaagccaatg agttttgaaa attctcgttt aaaacctaaa aacagtccaa
      181 tgacaacaat cgctaacaat accaatgact gtgcattatc caagaaccag ttgtacaaat
      241 tctgcccaaa attcatgtga ttgtcctcct ttccttaaga tatttttctc gataatagat
      301 atagcttttt acacgcctta ataattgaaa aggggttaaa tgctctccat agacggttac
      361 tcgattggta taattattat aaaatctcac cttgtaaaaa tcataggtat agatggtatc
      421 accagcttgc cgttttccct caataatgag agacgtatat ttccctgtat tttgtatata
      481 cgcttgaaac tcaatgccac actgaaaccc taacttcttt aattcttttg ttaaaaggct
      541 catgtaatca aatcctccat ggctagatgt tgttgttcaa ttaatttctc atgtttttct
      601 gtcagtttgg cttcctgaat catggtggag ataatggttg taccgttgag aacatctaaa
      661 atgcttgcga tttttaatgt aggggcgact tgatgatgta accagttcaa tgttcgttca
      721 aaagaataag gctctggctg tgtggttagg cgtaatgctc cacgattttt accgataaac
      781 cattcccaac gttcattagt tttccaatca ctccgacgtt tagtgtcgtc tttatctgca
      841 aagcggatat agcgattgat aatttcaaag gctgtttttt ccgcctgttg atgtgccaat
      901 aaatctttca tggcttgggt ggctcggtca ttttttaacc gaatttcaaa ccgattcttt
      961 acttctgcgt cttctaaggg aacatcattt ttcacgtatt gctcataatc tttctcatat
     1021 atacagaaat agacttcact ttttaatgag ccaatataaa gtgtgtttcc catgtatggt
     1081 ttttcatcac ggtgaaccaa ttcaccactg cgataatttt taaagcttcg aaagacggaa
     1141 atgcattctt cctgtttaca tttctttgcc aaatctggaa tattcaaaat ccctgtttta
     1201 tcgttgatag ctaaatccaa gcgtttgaaa atacctttat gttccatgca atcttgaaag
     1261 aaatcatacc agctccgatg ttgagccaat agaaagtttt caaattgccg acagcctttt
     1321 cctttcagtt ctaataaaac acctttactt acatcatgag agactaggac aacaatatca
     1381 ccgaaacgat agtgttcggg ataagaataa tagccaaagt cttcgtgtag catataatcc
     1441 atattcaaac gtaaaacctc accgataatt tgagccacat cattggtagg gaatcgaatc
     1501 cgtacatagt cgaataacat ctccaatggg ttatcgggat tgaagcgttc caaagcgttc
     1561 agtaaatctt ctttcacctg ttgagtaggg actgccttgc ctgtttcaat atccgatagg
     1621 taaggtctgg taatatgaga agcaaccgcc agtttatttt gagaaacgcc ataagccagt
     1681 cgcttttctt tcaattcctt aatccaaatt ttttcgtttt ccattcgcaa acctccaatc
     1741 taaaaaagat gtaaactatg acccttgagt ttacaccttt catattttcc gcaatccttt
     1801 gtaccatcaa cattggttga tgagaattgc ctgttttcag ttgtatttgt gcccccctgt
     1861 tagatagagg gggctaaagg attggcgtgg ctaacgccac accaatcaaa gcaagtctga
     1921 acctgtacct ttcacttcgt gcggtacagg ctcagattgc tttttttgaa tgacttttcc
     1981 tatttctcct aagaaatcgt acctttttgg tacaaggggt gtataaaact cactaatcac
     2041 actttttcct gtatccacgt aacctcttcc cttgatgggt tttaagaaaa attgtttttg
     2101 tgtatcgctt ccgaacatca taccaaagcc catttcagac atacgaccca aagccacacg
     2161 gaagttaaag ttgtcccgaa tcccatcact aaaatatttt gcgtctggac gttgacaggc
     2221 aagaattaaa aagaagcctg cttgtcgtcc taacattaca atttgtttga gtttatgaat
     2281 aacggtggta ctttcacgac ctagcatatc catcattgcc acatattcat caaaaataag
     2341 aaaattggca gacaaaccaa gataagcata attttctcct gttttatagt tagggtgttg
     2401 tttcatggtt tcactgcgat caatcatatc ttcgtaaaat tgattgatac atgccaacat
     2461 atcttcttta cggtaataga cattattcat gaccgcccct aagtctgcca aatcagcatt
     2521 tttagggtca agaatataaa gctttgcatt tgtatgcagg agagattcaa tcaatgtgag
     2581 aataaaatag gattttccac taccagtgcc cccagcaatt aacatgtgag ggagagaatc
     2641 gaactcccaa tagaccgatt ccatcaattt gacacgtcca tcttgagcta ccacgtcttc
     2701 aatgccaatt cgattgccaa tagtatcata aagtaggaca tactctacat aagaatcttt
     2761 caacgttttt tccactaatt cacaatacaa tcctgtttct agtttgtttt ctaggtgcaa
     2821 aagctgatct tgatatttcc ctaaggtgat ttctacatga atatggatta gtccatcttt
     2881 cattcgataa tatattttag gaaaatggcg gattcgttgt ttggttttct tgctgggtaa
     2941 atctttaaag aatccctctg attgagtttc ttcggcttca taccaatgat tttctaaaat
     3001 catacgagct aacttttgac ggtgctctaa ctgttttata gaatctatac gatagtgata
     3061 ataaagccag ctgataagtc cgcatacaaa aagagcagtg agaatactgc tccaaaagta
     3121 tggcgtatga agttgataga aaaatgtatc taactgaact gcttgccagt tcaccgattg
     3181 taacgctctc caatgaaaag ggagtaggac gactagaaaa actaccagca atgaaattag
     3241 tacaaaatga tagaccaagt gcttatcgtt ggctttgatt cgctctcctc tgtcatgaag
     3301 caatggcata aaaatcctcc tttccaccta tcaaaataat tgtgtattgt agatacgagc
     3361 ctgtttatca aaagttggtt gagaaatggc ttcatatttc tgataagcga ttcgcttttc
     3421 aaagcgtaaa tttttttctt tcggataata atgacgtcga tagagagtgt gtcctaacag
     3481 ttttttcgct tcttttgtca acggatagag atagcgaaac atgcgtccat tgattttctc
     3541 aatccctttg tattcgcaaa atgcctggga aagccaatgc tttttctcta cctgttcaaa
     3601 gcgagcattt tcctctaata aaaggcgagc acttcgaggg tgtatctttt cccatgactg
     3661 cttatcacga tagacacttg tcttgaaata cccgcagtaa aagaagttgg acgcttgata
     3721 gacatagcca cattttccct caattccatc tgccagtgta tataaaaaat gacagtccgt
     3781 atgttctttc aaccacttaa ttagagctga aagagcttga ctgccaaaat aattggtttg
     3841 attcatttct ggaagaaaac acatctttcc aatttctaaa taatctgtgg tttgaaggga
     3901 actgtcggga aagagtttgc gtatggtttg taggggttgc gtaccccagc ctaattccac
     3961 aacgcctaat aatttttctt ctgaaaaaat ccctagaaag tatttacaca atctagggag
     4021 tatctttgaa taatggtatt gccgaataaa agaaatggct tcatccttcg gaatttcttt
     4081 caaaatcaat ggtgtgtttg tcataagcaa cacctatttt tcactttcgg acgcttcttt
     4141 tttatatttc tgatgaggtt ttgtctgtcc ttttaataca agatcgtctg ctttcacgta
     4201 ccaatcaacg tctgcccctc gaaaagtagc cgtcgctacg gtatctgcca caggattgat
     4261 taattccact tctgcgttgt agtcaaattc tttcaaagga acagaagcgg ggatactgac
     4321 ttgtatcatg cgtccttgtc cttttgattt taagtcatag gtacgttctt taatttcttc
     4381 ggaaaccgta ccatcttcat tctggatacg aacctcacga cgcaaggcgg aaaatttcaa
     4441 tgtgccaaat gtctggtctt tttcaatcac aataccttct gctaatctca tattttttca
     4501 cctttccttt cttatgcttt caccatatcg tctgcgtgca taatgtaatt ggtaaatcct
     4561 cgattgccaa ttttataacc ttctgcggta atatgtgggt tgatgagttt gactttttcg
     4621 tcggattcaa aatgtttctc gccagcttcg gagggaagaa tgacaacaat atcatctgct
     4681 cgttgaatcg ttgagtataa attgtaacta cgagagagaa tggttggtct gccatttatt
     4741 cgacgttgtt caatgttccc ttcaccagca tattctaatt gtccaaatgt tttttccata
     4801 ttgggaatga catatttaag ttccatattt ttacctatcc tttctgtttt tatgtttgaa
     4861 tgaatcttat ttgtgtgatg gttttaaagg ggggagagcg accttttgat tcttgatttt
     4921 ttattttcat aagttcactt cctttcaaaa ttgggtaaaa aaatagacgc tccgtactga
     4981 gcgtccgatt cctactatat ttaaaacaac gactacctag gtattttaaa tattataatc
     5041 aaattctatt ttatctaata attgagtggg ataaagcaag tctcctttac tcgtaaaata
     5101 acccacctct gagaaaagaa ataaattatt ttttgctctt gaacaaatta cgtatagtag
     5161 ttttctcgct gaactaattc ctttatcaga aacattaaaa atttcattcc aatgaggaat
     5221 tttcccgttt agaagattaa tagcgataac agtatcatac tcatttcctt taactttatg
     5281 tgctgtagta agtgtgatac catctcgaga tttaaacatc ttcttaaaac tagatacact
     5341 actagataat gaattatttt ttatccgatc ttctacttcg ttaataaaaa atagatagtg
     5401 cttaaaaata gacgggtaat ttttctcgtc aatattaaga ttaacaaata tatgtttggt
     5461 aacaaatttg tataaatcaa caccgttgtc gtctccacta tacattcttt tcacactgtt
     5521 tattagctgt aataaatcta agtcaataaa ataatcttta acttctattc caaattcatt
     5581 ttttaatata attaaaatat cgtttgcaat tagctttctt cttcgaacct tttcacctga
     5641 ttcagtaaag tacaaaattg ctaacttgaa aaataaacta tgattattta ccttaattgg
     5701 tgatatattt atagcatcaa aattgcaat
//
LOCUS       DACEZN010000159         5681 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEZN010000159 DACEZN010000000
VERSION     DACEZN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.159"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            133..1287
                     /locus_tag="IXH60_002417"
     CDS             133..1287
                     /locus_tag="IXH60_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1991306.1"
                     /translation="MEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSIN
                     GELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQ
                     REMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKA
                     GTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEY
                     RSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1385..2758
                     /gene="tet(L)"
                     /locus_tag="IXH60_002418"
     CDS             1385..2758
                     /gene="tet(L)"
                     /locus_tag="IXH60_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1991307.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3322..4584
                     /locus_tag="IXH60_002419"
     CDS             3322..4584
                     /locus_tag="IXH60_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1991308.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            4829..>5681
                     /locus_tag="IXH60_002420"
     CDS             4829..>5681
                     /locus_tag="IXH60_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1991309.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1954 a          805 c         1167 g         1755 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact ttctttctta
      541 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      601 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      661 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      721 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      781 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      841 tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
      901 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
      961 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
     1021 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
     1081 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
     1141 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
     1201 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     1261 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
     1321 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggaatt
     1381 aattatgaag tgtaatgaat gtaacagggt taaatacaac caaattttaa tttggctttg
     1441 cgttttatct ttttttagcg tattaaatga aatggttttg aacgtctcat tacctgatat
     1501 tgcaaatgat tttaataaac cacctgcgag tacaaactgg gtgaacacag cctttatgtt
     1561 aaccttttcc attggaacag ctgtatatgg aaagctatct gatcaattag gcatcaaaag
     1621 gttactccta tttggaatta taataaattg tttcgggtcg gtaattgggt ttgttggcca
     1681 ttctttcttt tccttactta ttatggctcg ttttattcaa ggggctggtg cagctgcatt
     1741 tccagcactc gtaatggttg tagttgcgcg ctatattcca aaggaaaata ggggtaaagc
     1801 atttggtctt attggatcga tagtagccat gggagaagga gtcggtccag cgattggtgg
     1861 aatgatagcc cattatattc attggtccta tcttctactc attcctatga taacaattat
     1921 cactgttccg tttcttatga aattattaaa gaaagaagta aggataaaag gtcattttga
     1981 tatcaaagga attatactaa tgtctgtagg cattgtattt tttatgttgt ttacaacatc
     2041 atatagcatt tcttttctta tcgttagcgt gctgtcattc ctgatatttg taaaacatat
     2101 caggaaagta acagatcctt ttgttgatcc cggattaggg aaaaatatac cttttatgat
     2161 tggagttctt tgtgggggaa ttatatttgg aacagtagca gggtttgtct ctatggttcc
     2221 ttatatgatg aaagatgttc accagctaag tactgccgaa atcggaagtg taattatttt
     2281 ccctggaaca atgagtgtca ttattttcgg ctacattggt gggatacttg ttgatagaag
     2341 aggtccttta tacgtgttaa acatcggagt tacatttctt tctgttagct ttttaactgc
     2401 ttcctttctt ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg
     2461 tgggctttcg ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca
     2521 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
     2581 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
     2641 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
     2701 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
     2761 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
     2821 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
     2881 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
     2941 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
     3001 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
     3061 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
     3121 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
     3181 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
     3241 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
     3301 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
     3361 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
     3421 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
     3481 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     3541 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     3601 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     3661 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     3721 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     3781 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     3841 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     3901 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     3961 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     4021 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     4081 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     4141 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     4201 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     4261 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     4321 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     4381 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     4441 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     4501 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     4561 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     4621 tggttttgaa cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt
     4681 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     4741 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     4801 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     4861 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     4921 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     4981 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     5041 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     5101 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     5161 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     5221 aaataagagt ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat
     5281 taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa
     5341 ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac
     5401 agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga
     5461 ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat
     5521 acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga
     5581 tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag
     5641 gttaatctcc tatggtggtt tgttaaaaga aatacataaa a
//
LOCUS       DACEZN010000160         5561 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEZN010000160 DACEZN010000000
VERSION     DACEZN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.160"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(25..144)
                     /locus_tag="IXH60_002421"
     CDS             complement(25..144)
                     /locus_tag="IXH60_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1991310.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IXH60_002422"
     CDS             complement(159..1343)
                     /locus_tag="IXH60_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1991311.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1600..1854)
                     /locus_tag="IXH60_002423"
     CDS             complement(1600..1854)
                     /locus_tag="IXH60_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991312.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1879..3021)
                     /locus_tag="IXH60_002424"
     CDS             complement(1879..3021)
                     /locus_tag="IXH60_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ1991313.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3081..4433)
                     /locus_tag="IXH60_002425"
     CDS             complement(3081..4433)
                     /locus_tag="IXH60_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1991314.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFPDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4516..4851)
                     /locus_tag="IXH60_002426"
     CDS             complement(4516..4851)
                     /locus_tag="IXH60_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1991315.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(4861..5235)
                     /locus_tag="IXH60_002427"
     CDS             complement(4861..5235)
                     /locus_tag="IXH60_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1991316.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(5248..>5561)
                     /locus_tag="IXH60_002428"
     CDS             complement(5248..>5561)
                     /locus_tag="IXH60_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1991317.1"
                     /translation="ELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLYS
                     SKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLVK
                     A"
BASE COUNT         1569 a         1354 c          925 g         1713 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggg tgagacatca
     1501 tggccggcta acgccgtcca taaacaggca cagcaccacg gaggatttct gcttcgcaaa
     1561 tcccccgcgg tcagtgccta tcagtggttt atttggccgt tactccgcgg taataccctc
     1621 aatttccaac tctgctttta tctcaaacaa gtattggcgg atatcaggtt ctttagcaaa
     1681 gtccgcaatt cgttccaatt cactgatgac ttgattcagc tccattcgag catccgtttc
     1741 ctcatcaatt acatacggat catcagggac tttcaccccg tttttgcgaa tatcaaaatt
     1801 gagccggtac agttcttctt ccaattccgg atctgactga atcttgccaa tcacccagta
     1861 gattccccaa tcaactcatc ataaatcatc tgtttttacc tcctttaatc ttctagcaat
     1921 atcaaaaatg accggaactg ttacagaatt tccagcctgc ttatacaact gactatctga
     1981 attgacttga gaagctcgat caaaagccca gtccggaaag ccctgaagcc gccagcactc
     2041 cctaggtgtt aactttcgaa tttgatactg atccgtgacg actgcttgtt ggtctcctgt
     2101 taataaggta tgcacacttt gttttcccag acggcctcga cgacttgtgg aattaggaaa
     2161 agccaggttc acactgttcc ctggcttcac ctcttgatat tctgtttcgt ttttccgaag
     2221 catgatcttc gttccgcctt gtttcgttga caaagtaggc gctgttccag ttatctcata
     2281 aacccgattg ccttgttcaa aatttcccag taacataccc gctacagaaa ttccatcatc
     2341 taccggcaat gcgattttga ttccttcatc accggtcaag gtaggcgcta cgtcatctga
     2401 cccatagact tgtcccccca ttcctcgatt ggaagggttg atatttaata agtttctgat
     2461 gttttgatca gatgttggag acgatccaat gataggaaat acttgttcgg tacatgcgtc
     2521 tcgagaatgt ccgataagga agatccgctc acggttttgg ggaacgtagg cctttgagtt
     2581 aagcacttgc cattccacat cataccccaa tccatccagc gttcggagga tcgtctcgaa
     2641 cgtagcccct ccttcgtgat tgagtaatcc ccggacgttc tcaaggaata aaaacttagg
     2701 ttggagaata gcggcgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa
     2761 tccttgccgc tttcccgcaa ttgaaaaagc ttggcacgga aatccaccgc aaaggatatc
     2821 cactggtccg agagattgaa taaactcgtc tgatacactt gtgatgtcat gcatttccac
     2881 ctccttactt gtatcatgga ttgccttata actctggcga gcaaaatcat caatttcaca
     2941 aaagccgata cactggtgac ccgcttgttc catacctaag caaaagccac caatcccagc
     3001 gaataaatct aaaaatgtca taaatcacct cgtaaaaaaa gcccaggatt cttgacactc
     3061 ctaggctaca tactattatt ttatccttcc gtattaacgt ttaatccacc aatgtcgcta
     3121 atttccctta agaaatcata tccttttggt acaagtggcg tataaaactc actaatcaca
     3181 ctccctcccg tatctacata gcctcgacct ttgatacgct tcataaagaa ttttttatca
     3241 acctcgccaa acatcatgga atatcccagt tcactcatac gacccaaagc cacccagaaa
     3301 ttaaattgat cgcgaatccc gtctcctaaa tatttcgcat ccggtctttg acaagctaga
     3361 atcagaaaga aaccagattg acgacctaac atcacgattt gtttgagctt attcaaaatc
     3421 accgcacttt ccttcgtcgt taacatttcc atataaacca cgtattcatc aaagattaaa
     3481 aagtttggtg gaagtccaag aaacgcataa ttctctcctg ttttgtagtt gggcatttct
     3541 ttcattgcct tgctacgagc catcatacgt tcatagaaat cttccacaca agcagaaatt
     3601 tcttcctttt gtgaataaac atgaggcatc accgtaccta aatcagctaa atctgcattt
     3661 ttgggatcga ggataaacaa ttctgcatct gacttcaata gtgcttcgat aatggtgagg
     3721 aggaaatagg tcttccctcc acctgtgcca ccagcgatta acatatgggg taaagaatca
     3781 taagcccata cttgattttt catcaatcgc agagtcccat tttctgccac tacttcgtct
     3841 atcccaattc gattggcgat catatcatac agtaaagtat actccacata ggagtctttg
     3901 agttcctttt ctactaactc acaatacagt ccgctttcta acttcttttc caattttaaa
     3961 agctgatctt ggtatttccc aagtgaaatt tgaacgcgaa tagacagtaa tccatctttc
     4021 attcgataat agattttggg gaagtaactg atagtctctc tggttcggct actattcaaa
     4081 tctttgaaaa agccttcact ttgagtttgt ttcacttcat accaatggtt ctctagaatc
     4141 atccgagcta gcttttgtcg atgccacaag cttcgccaat gatcaggaaa aaagtgaata
     4201 taaagcaata tcatagctcc tgaaaaagcg agacttccaa gcagacatac taaatctttt
     4261 atagaccaaa cgatttcaat agtagaaagt gatagcttag aaatatcaaa ttgtctaaac
     4321 tcttgaaaat aaaagacgct catccaaccg atgaacagag cgagtagcca gccaagaaca
     4381 aagtgataaa ccaagtgctg atctcctgcg cgtatgcgat gtcctttgta cattttgatg
     4441 gtttccacct ccaatcgaaa aagcccaata ggatttcatt aggcaatttc aaaaagttat
     4501 gctagtcatt tactgctacg aggaggggag ttgtttcgtt tcaaccaatt gatttaaaaa
     4561 gccttcaata gcatccggca tctccacacc aatttcttcc aaaatggatt ctgcctgccg
     4621 acataagttt tcatttactt cgacaactat aaagaaagac tcttcttctc ctaaccagtc
     4681 tagtacatca gagtcttctt cactgtcgct attttctcca agtaaacctt gatgtataat
     4741 cagaaacatt ttttcatcag aggttaatgc tttaacgagt tgttcctcag tcagctcgcc
     4801 ttttccatat tgatcgagac aatctaaaat atgttcaaca ttaatttcca taatcttcac
     4861 ctatttcttt ctttcatttt ggttcgtagg atttccttga ttcggctgcc gcttcaagac
     4921 taaatcatcc gccttaatga accaattaac ggtagttcct tctcgaaaaa cataattggc
     4981 aacggtatcc acgattggat tgaccaactc tacctctgca tcataagcaa actcgcgtaa
     5041 gggtacatta gctgggatac taacttgaat cattcgccct tgtgcagagg attttaaatt
     5101 ataggttcgt tcttttacct cattaccaat tgttccatcc tcattttgct tccgtacttc
     5161 tcggcgtaat gcggaaaaac gcaacttccc aaatgttaaa cctgaatcta cgacaatacc
     5221 ttctgttaat ctcatcttct tttcctccta tgctttcact aaatcatcaa catacaacac
     5281 gtaatcagta aacccacgat tctcaatctc atagcctttg gcaaccaaat ggggattcac
     5341 ggcttttagc ggttggtctt gatcaaaatc cttttcaccg gcttccgcag ctactaccac
     5401 ctcaatatca tcggcccgtt gcttactgga atacaaatga taactgcgac caatcaccgt
     5461 agtattccgt ccatatcctt ctgttataat ttctccttca ccggcataac ttaactttcc
     5521 aaaggtttca gccatatctg gcacgacaaa tttcaattcc a
//
LOCUS       DACEZN010000161         5513 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEZN010000161 DACEZN010000000
VERSION     DACEZN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.161"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..198)
                     /locus_tag="IXH60_002429"
     CDS             complement(<1..198)
                     /locus_tag="IXH60_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991318.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLI"
     gene            419..1792
                     /locus_tag="IXH60_002430"
     CDS             419..1792
                     /locus_tag="IXH60_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1991319.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            1867..2979
                     /locus_tag="IXH60_002431"
     CDS             1867..2979
                     /locus_tag="IXH60_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1991320.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3051..4688)
                     /locus_tag="IXH60_002432"
     CDS             complement(3051..4688)
                     /locus_tag="IXH60_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1991321.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(4856..5464)
                     /locus_tag="IXH60_002433"
     CDS             complement(4856..5464)
                     /locus_tag="IXH60_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1991322.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         1465 a         1109 c         1104 g         1835 t
ORIGIN      
        1 aatcagccaa ttttttttag gcgacatgcg tattgcatag ctgactgcat atagacaggc
       61 tactcttcgt ttaaagtctt cctgattcgg gtcgccgatc cctctgatgc taaagaatga
      121 ttttttagga acctctagta tttgcgggct ttttttcata ctatagctct tgtctttacg
      181 ccattcaatt ttttccattt ttcttcctcc gcgaaattcc atttagttat ttattatcat
      241 acctcttttt gagtgacttg gctaatcgtg ttgacactat ttttcagttt gtgctagaat
      301 aagtgaagta aataaatcgt acagataaca cgtttcggcg tgtcgagaga gaggcggttt
      361 ctatttttat aatagaaatc actctctttt tttacgaaat tttttagagg tgatgtaaat
      421 gacaaaacaa aaacagctgc ttttagtacc tgcagtaatt gcaacaggga tgatgtcttt
      481 tgcgggcgta ttgatcgaga cggccatgaa tgtaactttc ccaactctta tcagacagtt
      541 tgatttaacg actgcacaag tccaatgggt aacaacgatt tatcttttga tgatttcgat
      601 cattgttccg ctgtctactt atttgaatcg caattttagt ctcaaaaaac tctttttagc
      661 ttcaaatctg cttttcttgg caggtataat gatcgatttc ttctctccta ccttcatggt
      721 cctattgttt ggtcgattgc tccaaggtgc agcgacaggt atcgctcttc cgttgatgtt
      781 ccatattatc ttgacatttg cccccctcaa tcgacgaggc gcgatgatgg gtgtcggtac
      841 tttgacaacg gccattgctc cagcaattgg ccccacttat ggaggcatta tgacttcaca
      901 ttttacatgg aatcatatct tcctgtttct tgtgcccgtt ttgtttcttt ctttgattat
      961 cggctggttt gctattcccg agatggcagt acaaaaaagt ggttatttgg attggataag
     1021 cgttttagga atttgtcttt tgtttagtgg aagtctaaca tttttgagta cgttgggaac
     1081 agtttccagc tggattgctt taatcatcgg cggtattggc tggttccttt tttactatcg
     1141 aagtaaaaaa gtggagcatc cactgatccg actgtctatt ctttcaaata aaacattccg
     1201 cttgtttttg attggcttct tagtttatca gtttttatta cttggtgtat cttttgtctt
     1261 gccgaatttc cttcagatcg ttcaaggaag tagtcctttt gtcgctggat tagcgatgct
     1321 tcccggcgca gcggttggtg ctttgctttc tcctttttcc gggaaaatgc tggatcaata
     1381 tggtccgaag aagccaattt ttgctgggct gattctttct ttgattggtt ggctagcatt
     1441 attcctactc ataggacacg cttcagtggg tgtattgatt gcttgtcatg tgttttatat
     1501 gatcggtatc ggattatctt acagcaatat gatgaccacc ggaatgaatg cactgaatga
     1561 agaactacaa ggagacggga acgcaatgtt caataccttg cagcagtttt ctggcgctgt
     1621 ggctacttca ttagtagcgg tcattatcaa ttacgtccag catcatactt cccataacta
     1681 tgaagtgtct actacattag gatcaaaagc agcattaggc gtcttgttgc tgttgcttct
     1741 cgtaagcttt gctcgattcg cctattatct gttctttgct aaaaaagcgt agattccata
     1801 tacggcaggt gttgcatctg ccgttttttt gtgtgaaaat ggttgtttgt aaaaggaggc
     1861 ttttttatgg aaaaaattgc cattatcggt ggcgggatca ttggtatgac cctagccagt
     1921 tacttagata catcaaaatt caatgttgct ttatttgatg aagggacagg acaggcaact
     1981 aaagctagcg ctgggattat ctccccttgg ttgtcgaaac gcagaaacaa acaatggtat
     2041 cgtctggcta aagacggcgc tgcttttttc cctaaactta taaaagattt ccaacttgat
     2101 caaacgattt acgcacaatc agggacactt ttgcttcgac ctgaaaaaga tttagctgaa
     2161 ttggctgttc ttgccgagaa acgaaaagaa gaagcgccag aaattggaaa catcgaatgg
     2221 ctttcacctg aacagacatc tcaagcactt ccgttattga aaccagcagt ttcactgcgt
     2281 atcagtggtg gtggtcgtct ggatggaaaa gcatatttag aacatcttgg tcagcgttta
     2341 gcacaaaaac aaatggagca gatccaggca aaagtcactt tgcataagaa gagcgattct
     2401 tggctcatca agggaccgtt tggagaatac acagctgatc gtgtaatttt gacacctggc
     2461 ccggcactta agcctttatt acaagaatta ggctatgctg cagatatacg tccgcaaaaa
     2521 gggcagctct tagcatttca gacatcttat aagatgagtc aagactggcc tgttgctatg
     2581 cttgacggag aagctgattt cattcctttc atggatggaa agatcctttt aggtgctacc
     2641 catgaaaacg atcaaggctg ggatttggct cctactagcg aagcttataa gggattgact
     2701 aagagtacag aacagttctt gactgatcca gatcagataa aaaactggcc atacgagttc
     2761 cgtgtaggta cccgtgctta tacgtcagat ttttccccat ttttcggcgt actgccagat
     2821 gatccaagct tactggctgc tagcggtcta ggctcttctg gcttgaccac aggtccttat
     2881 atcggctatc tattagccaa cttcttaaat actggtcatt ggcctacgga agattatcaa
     2941 aaggacatct ccacctatat tacgaaaaat ccatcataga aaaacagctt tttcaagtaa
     3001 ggatcccgag cgatattgcg catggattac ttcttgaaaa agctgttatt ttagttcaat
     3061 ttctgaatca aattctccaa actttctttt gcttcatctg gcaagagatc ttttcctatc
     3121 tgagcttcaa tgaatcgtaa acgaatatca agtcgttgtt tcaccagatt tttataggac
     3181 gtatcctcaa aagaaacagg aaaatctggt ataggtaaat agtggatgtc ttcaaatgga
     3241 ccaaacgact tgaattctgc cgtttgttgc acgattcttt caacgattga caatgtcgtt
     3301 tcaggtttga tttttttatc atcgaaatat ccagtattca ggacatagca ggctgtttgc
     3361 tgtttcaaaa acaactctcg aaaagcttga tagtcttccc taagaggata gacacggaaa
     3421 ggattagcaa atggttcaat gactagctca tctagctgtc tttttgtagt gatattttcc
     3481 gcgtctgatc gttttgttgc taatgttgca ccaaaagacg ctgctaaaac tgggtcattg
     3541 atcttgataa ttggcggaag cgaatcatct ttcatgatcc aaaaaacagc atcgatcttt
     3601 tcgctaagat gatcgactcg gtcgggtgtc gcataacgtg atttgactgt ccgcccattt
     3661 ccgtttcgta tatctcctgt tactaggact ttttctcctt tacttgtaca tgtcacgccc
     3721 acattctgac aagtaaggaa agaagaaatg gccggatcat cagacggata gtcttgtgtt
     3781 ttatcaaagt aagaaggttc caaggcagta gcaaaaccat tttttcgaga aataacgaaa
     3841 gcatcgtcat gcaagatcga aacagagtga ccttccgtat gttttgctaa ggtaatcgtt
     3901 gattttccag aaccggacaa cccaaataca gccatcgtat aggtctggtc attactagca
     3961 tattgtttca tcccaccgtg gcaggcaata aagccattgc gatttcccaa tgcccaagca
     4021 agcgtcaaag tcgctttttt taattctcca aagtaacgta atcctagcac agccgctgta
     4081 ttatggagag catcaaacaa aatgagtcca tttggaaatg tttcatgtgt ccattcggga
     4141 tcacaaaaga tataaagatc tccctcatca aaggaaacag acttttgata agtctctaag
     4201 atcttcgaat tcgctggctg aaaattcagc aaataagaat acaaattgtt ctcaaatcct
     4261 tctggaatca gcaaatgact ttgcacagaa aattcttcag ataggccaac gattactttt
     4321 gtatggtaaa actggcgctt gcgactttga tacacagctt ctcttaaaat tttctcgtat
     4381 ttgctttgtt ccttgtctaa gtcctcaaag atccgccgag cacttgctgt tcttcccaca
     4441 atgcttccat cattgaatac tagtgtccta acatcagctg gtaaatctag ttcttcagca
     4501 tgtgagattg ttaaatctgt gatgatcgtt cctggactcc cttttgctag ttgataagct
     4561 gtcttcctat caaaaacttc atgaacatta ttaccgtaaa atgtcgtttc tacaatcgat
     4621 cgaatactgg ataacagtgg atttccactt gtgatttcgt ttaattcgag actctctttt
     4681 gtactcatgg ctcctcctca ttccctaaac aaaaaaacgt tttctttatt atacattatt
     4741 cgagttttta tttgtactgc tgcgctgtgg gaagcaaacg agttattttt cacacaaaaa
     4801 aaccgagaac ttgaataaac aagtttctcg gtttcatcat tttcctttta tcggcttagt
     4861 accagccgtt tgctaaccag aatgttttag cagcatccca tgaaccataa cgagatgtta
     4921 catattgttc tgctacacgt tcttgattgg cagctgaata gtcgccgttt aagtatgaag
     4981 catctaattg ataacggccg atgtaacgtc cgttagtagc tgtgtatgaa ccgcctgatt
     5041 ctttttgtgc gatccattct tttgcagagc ttgaagttgg cgctgttgca gcagctgggg
     5101 cagcaggagc tacttcaact gtttcaacag gtgcaacgta tgtatgttct tgcgttgctg
     5161 tttcagttgt agatgctgta ttttctgttg ttttgttttc tgttgttgct gaagaatcat
     5221 ttgaagttgg gattgttaat tgttcaccaa cataaatgcg gttgatgttt gcgatcgaat
     5281 ttttttcagc aattgcatcg atcattgagt tgtctcctgc aaatttttgt gagatcttag
     5341 ataatgaatc acctgatttt actgtataaa cttcatcagc gtgagcattt gtagttccta
     5401 caaacattgc agtagcacca gcagcaaaag ttgtacctaa aacaattttc ttaaatgaat
     5461 tcatattaaa ctcctttttt tctccgtttt ttattctttc gacgctatta act
//
LOCUS       DACEZN010000162         5477 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEZN010000162 DACEZN010000000
VERSION     DACEZN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.162"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(39..405)
                     /gene="ssrA"
                     /locus_tag="IXH60_002434"
     tmRNA           complement(39..405)
                     /gene="ssrA"
                     /locus_tag="IXH60_002434"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(576..1349)
                     /locus_tag="IXH60_002435"
     CDS             complement(576..1349)
                     /locus_tag="IXH60_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1991323.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1493..1843
                     /locus_tag="IXH60_002436"
     CDS             1493..1843
                     /locus_tag="IXH60_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1991324.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1824..2540
                     /locus_tag="IXH60_002437"
     CDS             1824..2540
                     /locus_tag="IXH60_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1991325.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2540..3988
                     /locus_tag="IXH60_002438"
     CDS             2540..3988
                     /locus_tag="IXH60_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1991326.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4058..4567)
                     /locus_tag="IXH60_002439"
     CDS             complement(4058..4567)
                     /locus_tag="IXH60_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991327.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4557..5282)
                     /locus_tag="IXH60_002440"
     CDS             complement(4557..5282)
                     /locus_tag="IXH60_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991328.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1861 a          955 c         1095 g         1566 t
ORIGIN      
        1 aaggatttaa gcatctagcg aaatctgctg aaatctcaca atggagacgg cgggagtcga
       61 acccgcgtcc aaacacatcg ccacttaaaa atctacgttc atagtcaact catttaagga
      121 ttcactttac agcttgccga gtgacaggca ttctgtatcg ctagtctgat gttctctttt
      181 tcaacttaca gacggaaagt tgaaacgtat cccactaaag ttaggaccct tactcgagca
      241 catgggcgat gccgagagga tctacgctaa ctgtttttag gcagctaaag cgtaagagtt
      301 gttttcgttt ttagcagtta tatttaactg taacgttttt acgtagacgt aacctacgaa
      361 acgcaattca agctcgactg tgcctgtcga atccgtaacg tccccgttag ctaacaaggg
      421 ttacgtgtat ttttcataca aaattagtat agcatatatt gggataaatt caataaaaaa
      481 gcgaatcata tcccattatt tatataataa ctgccccttt cttaagaaaa aataagcgat
      541 tttcttaaga aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa atacttcaat
      601 gcgtggacaa ttccgccttc tgtatttttc tttgtgataa aggtagaaag ttcttttaat
      661 ccgtcatagg cgttgcccat agcgattttg ttgtcgcaag cttccaacaa ggcaaaatca
      721 ttcggaccat cgccaaatcc aaaagtagga actttttcca gctgaagttc tttttgcaga
      781 cgtttgacac ctgccccttt tgagattcct ttcttcactg tatcgatcga gtaaggacca
      841 ttccgataaa aagttagctc aggaaaatgt tcgtgatagt attcgtcacc tgattgagat
      901 aagatcagca acatatttac tgtgttttct ttaaagccta aaggatcaat ttccggaaca
      961 tctgaatgaa tgaagtcgta agcattgatc attgtttggc tatgaccggt acaccagatt
     1021 ttctgatcgt tatagtaaga aagttcatga ccgttcaact gtacatgatc atacattttt
     1081 tgacattctt ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac tcgaatgaaa
     1141 gaaccattca tggtgaccgc agaagtaatg cccgcatctt ccatgataga gtggatttct
     1201 gcttctgtac gtcctgtagc gatgacgggg agaacattgt tttcttttaa tgcgttcata
     1261 gcttttgcca cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc atcaagatca
     1321 aagaaagtga ttgcacgata atttgacaca aataaaactc cttttttatt ggaataagtt
     1381 tatcttaaca aagaatgaac aaaagacaaa cctttcgtga agaattcctg aacatattgg
     1441 ttcagtcaac aatttagagt atactataag aagagaattt aggagggaaa gaatgatttt
     1501 tttcagtatt ggtatcttat tcttaataat tggcagtctt tttcggattc tgccctcaaa
     1561 aggaaatctg cctttttatg ggtatcattc gcctcttgcc gcaaaaacag acgctcattg
     1621 gcggctggct caaaagacaa gcggaaactg gttttttctt atgggcttac tgatggcatt
     1681 gatcggctat tatttaaaga catctgggca tacgaattat tttctgattg aaatgcttct
     1741 gctagtattt ccaattatgc ctatttttat tatgacagaa aagaaattac agaaatatga
     1801 cctggaaact ggagggaacg ataatgaata ttttaatgat tgaagataat caatctgttt
     1861 ctgaaatgat gcagatgttt ttcttaaatg aaggttggga agcaactttc aagtatgatg
     1921 gaaaagaagg gctcgatgcc tttttagaaa acccacaaca ttgggatatg attacactag
     1981 acctgaattt gcccacactg gacggtatgc tagtaggaag agagatccgc aaagtttcaa
     2041 gtactgtgcc gattatcatg ctgacagcta gagattctga aagcgatcag gtgatcggct
     2101 tagagatggg ggcagatgat tatgtcacaa aacctttcag tcctctgacg cttattgctc
     2161 gaatgaaagc tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa gcagtagttt
     2221 cagatgatca ttttgatatc gagactagtc atttcaagat gaatacgaaa acacgagaag
     2281 cgtatctgga caaccggcca attgaaggac tgacgcctaa agagttcgat ctattgttta
     2341 ctttagcaaa aaaaccacgt caagtattct ctcgggagca gttgcttgaa ttagtctggg
     2401 attatcagta ctttggtgat gaacggacag tggacgccca tatcaagaaa ttacgacaaa
     2461 aaatcgaaaa ggtcggacca caagtcatcc aaactgtttg gggagtcggc tacaaattcg
     2521 atgattctgg tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt tctgcggaat
     2581 ggtcgcgttg atcttattga tcgttggagt ttcttttaca caactgacaa aacaaacgat
     2641 tgaagaaaac aattaccagc aactgtttgg atatgctgaa tcagttgaaa agacaacgca
     2701 aacctatgcc gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa atgcattgct
     2761 gctgacagag caagtactga cagaacaaaa tgtgaacttt atatttatcg ataaaaacga
     2821 acgtgtcatt tatccgacta atacagcttt actgaatttt accttaacag ctgatcaatg
     2881 gaaaaatctg aggaatggac gtcaagaaaa attcacatcg aataaaaata ttttaggaca
     2941 agcacaggcg acctcttacg cacttgtgcc atttaatttg aatcatgaat tctatggagc
     3001 acttgtagtt agtcaacctg cgcgaaatat cgataacagc gttcgtccaa ttacactgaa
     3061 cctgttcaaa ggatttattt tttccagtat cgttgcagtt attgccagct atgcttttgc
     3121 agcattccaa gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag tgacgaatgg
     3181 taattttgat gtacagctcc ccgtacatga caaagatgaa tttgatgagt tagcagatga
     3241 tttcaataaa atgacgaatt cattgaaaga atctcaagca gaaattgaag aacaagaaaa
     3301 tcgtcgaaga caatttatgg cagatgcttc tcacgagatg agaacacctc ttacgacaat
     3361 caacggactt ctggaagggt tagaatacaa tgcaatccct gaaaatcagc gagaaaacgc
     3421 gattaaattg atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg aaaacttgga
     3481 ttatgaaaaa atccgtacga atcaaatcag tatggtcatt aaaaaattca atggaacaga
     3541 aacattgaaa aatattgttg cacagttaga agctaaagca gaagcagcag gagatacatt
     3601 gacattgaaa gccgttgatg atattgatgt ttacgctgat tatgaccgat ttgtccaaat
     3661 catggtcaat attattcaaa acgcgataca gtttactgaa aacggccaaa tcacgattac
     3721 tttagaaaaa ggctatcttg aaacgatcat cacgattgaa gatacgggaa ttggaatgtc
     3781 tgaacaacaa atgaaaagta tctgggatcg ttattataaa gtagatcctt caagaaaaaa
     3841 tacaaaatat ggtgaatcag gattaggttt accaatcgtg cagcaacttg tacgcttgca
     3901 taaaggaaaa ttagaagtag aaagcgaact aggaaaagga acaaaattcg ctgttcgttt
     3961 gcctgacgta gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc atgaatcgat
     4021 cttctatacc gcttgttttt actataaaat gattgattta gcccgcgggc tctaaacgat
     4081 caatataagg actttgagca atagtagcat catgagtaaa gagtaagata ccttgatttt
     4141 cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat acactaagtt
     4201 catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca tgaagtaatt
     4261 gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc aaggaaggag
     4321 agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt tccggtacat
     4381 ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt ttttggatga
     4441 tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc aatattttat
     4501 gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc aaatattcag
     4561 gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaaagt gaaagggaat
     4621 agactaagaa tgagacagca tccactgagc ccaataagag tttgggcaaa tcccttaagt
     4681 atatctgtga aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg ttgatccatc
     4741 ggaattgtaa gtccgcttac cgaagtcaag atatcacgga tatctggcag ttcagtagtc
     4801 tttaagacaa gaaaattgat cctttgaaga agcgcttcat aaactgactg gaagcttacg
     4861 atccacaata aaaccaagat acaacaaaga aagagaggaa agaacagctg ccagaaaata
     4921 aaaaggaata atttggtttt agaaagacca gtcaggtgcc aattgatcaa ttcctgcttg
     4981 ttttgcctga ctgaatagta aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa
     5041 ctgaatatgc cttttatata aaatcggcta gctaattcta aagccggaag aagctctgta
     5101 gatgcagaag cgccaataaa tgaaagccga tcttggactt gattggctaa agccgattgc
     5161 aaatggtaaa gagtcaggca tgctatcaat aaaaaagcag aaaagcttga aaataaacaa
     5221 tagagtattg tctgcttttt gtaataaatt aatcgagcag tagcatagcg gattattttc
     5281 atacttttca cctctataaa aagtatagaa aatgaatatg aataaagtat gaaattcctt
     5341 tagtgaaatc acgaattaaa accagaaaat attttgtaaa aagcaagagg ttgtgaagaa
     5401 gctgccctat tctattaccg tagatgcaag cttttgaaca ctgtttattc gaaaagagga
     5461 gcagcgacaa taatttt
//
LOCUS       DACEZN010000163         5463 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEZN010000163 DACEZN010000000
VERSION     DACEZN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.163"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..933
                     /locus_tag="IXH60_002441"
     CDS             <1..933
                     /locus_tag="IXH60_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1991329.1"
                     /translation="RWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLA
                     IDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHI
                     ESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAM
                     LIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVT
                     QNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCL
                     ISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            936..3119
                     /locus_tag="IXH60_002442"
     CDS             936..3119
                     /locus_tag="IXH60_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1991330.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3116..4117
                     /locus_tag="IXH60_002443"
     CDS             3116..4117
                     /locus_tag="IXH60_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1991331.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4132..5046
                     /locus_tag="IXH60_002444"
     CDS             4132..5046
                     /locus_tag="IXH60_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991332.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5291..5407
                     /locus_tag="IXH60_002445"
     CDS             5291..5407
                     /locus_tag="IXH60_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1991333.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1727 a         1048 c         1154 g         1534 t
ORIGIN      
        1 agatggaaag aaaccttgcc agagatttcc catgaaatca atatcgtcaa tctgacttct
       61 gatgagaaaa acaaaggctt acttgaccct tatgtaatta tgaaaaatac caaagattct
      121 gaatcactgg ctattgatat tttgacattc cttacgggga tttcctctcg tgatggggaa
      181 cgcttcccaa tccttagaaa agccattcgt gcagtaacca atagtgaagt gcgagggttg
      241 atgaaagtga ttgaagaatt acgggttgag aatacgccac taagtaccag catagccgac
      301 catatcgaaa gttttacaga ctatgacttt gcccatctgc tttttagtga tggttatgta
      361 gagcagtcta tcagccttga aaaacaactg aacattatac aggttgccga cttggtactt
      421 cctgacaagg aaacttcctt tgaggaatat accacaatgg agttactatc ggtagcaatg
      481 ctgattgtca ttagtacctt tgcgttggac tttatccata cagaccgaag tattttcaag
      541 attgtagact tagacgaagc atggagcttc ttacaggtgg cacaagggaa aacactatct
      601 atgaagctgg ttcgtgctgg tcgtgctatg aacgctgggg tatatttcgt gacccaaaat
      661 acagatgacc tcttagatga aaaactgaaa aataacctcg gcttaaaatt tgcctttcgt
      721 tccactgacc ttaacgagat taaaaaaacc cttgcctttt ttggtgtaga cccagaggac
      781 gaaaacaatc agaagcgatt gcgtgatttg gaaaacgggc aatgccttat cagtgattta
      841 tatggtcgtg tcggtgtgat acagttccac cctgtatttg aagaactgct ccatgccttt
      901 gataccagac cacctgtgcg aaaagaggtg taaatgtgaa accatcaata ttaaacagaa
      961 taaaatcaaa ctggacgctg aaacgtctag gtaaagtggc aatgacagtg gctttcacac
     1021 ttgtgattgc catttttctt ttagccatgc tgggaacggt ggttcaagct gcgggcttgg
     1081 tagatgatac ggtcaatgtg gcaaatgaat acagccgata cccacttgaa aactatcaac
     1141 tggattttta tgtggataat agctggggct ggctaccgtg gaactggtcg gacggaattg
     1201 gaaaacaagt catgtatgga ctatatgcca ttaccaattt tatttggaca atcagtcttt
     1261 atgtttccaa tgcgacgggt tacttagtac aggaagccta ttccttagac ttcatttccg
     1321 ctacagcaga ttccattggc aagaatatgc agacccttgc aggggttacg cctagtggac
     1381 tatcaacaga gggtttctat gttggattcc ttttactctt gattttggtt cttggggttt
     1441 atgttgccta tacaggactg ataaagagag aaaccacaaa ggcaattcat gccctcatga
     1501 attttgtgct ggtttttatc ctatcggctt cctttattgc ctacgctccc gactacatta
     1561 aaaaaatcaa tgacttttca tcagacatca gcaacgctag tttgtcactg ggtacaaaaa
     1621 ttgtcatgcc ccattcagat agtcaaggca aggacagtgt ggacttgata cgtgacagcc
     1681 tattttccat acaggttcag caaccgtggc tactgcttca atataacagt tcagacattg
     1741 aaagtattgg tattgaccgt gtggaaagcc tgctctcaac cagtccagat tccaacaatg
     1801 gggaagacag agaaaaaatt gttgcggaag aaattgaaga tagaagcaat accaatatga
     1861 cgattacaaa gacgattaac cgtttaggta cagtcttctt cctatttgtc ttcaatattg
     1921 ggatttccat atttgtattc ctattaacag gaatcatgat tttctcgcag gtacttttta
     1981 tcatctatgc tatgtttctg cctgtgagct ttattttaag catgattcca tcatttgatg
     2041 gtatgtcaaa acgagccata acaaagctct ttaataccat tttgacacga gctggaatca
     2101 cattgattat tacgacagca tttagtattt caaccatgct ctatacctta tcggctggtt
     2161 atccgttctt tttgattgct tttctacaga ttgtgacctt tgcaggaatc tactttaagc
     2221 tgggcgattt aatgagtatg ttttctctac agagtaacga ttctcaaagt gtgggaagtc
     2281 gtatgatgag aaaacctcgt atgcttatgc acgctcacat gcaccgtcta cagcggaaac
     2341 ttggacgctc cgtgactgct ttaggggctg ggtctgccat tgccagtgct acgggtaaaa
     2401 aaggacagtc gggttcggga agttctgcga gtacacaagc agatcactct cgaccaaacg
     2461 ggcaggaaaa atcaacacta ggaaagcgta tcggtcaaac catcggtaca gtagctgata
     2521 ccaaagacag aatggtagat actgctggta atttgaaaga acaggttaag gatttgccga
     2581 ccaatgcaag atatgcagta tatcaaggaa aatctaaagt gacggataat gtccatgatt
     2641 taaccagaag tatttctcaa accaaagcgg ataaagccag tggacgcaag aaacagcagg
     2701 aacaaagacg gaaaaccatt gcggagcgtc gctctgaaat ggaacaggtc aaacagaaaa
     2761 aacagccagc ttcttccgtt catgaaagac cagccactaa gcaagaacaa tctcatgatg
     2821 gacagacttc aaggcaaaca actgtacagg cttcctatag ggaatctcaa caagccaaac
     2881 aagagcgtcc aacagttaag tccgattctt caagtttaaa aacggaacgt caaagtaata
     2941 cagttcaaga aagaacggtt caaaagccag taacttcaac tgcaccaaca gatagagctt
     3001 cacaacgttc aatcacaaaa gaacgtcagt ctactgttca aagagtacca ctacaaaata
     3061 caaaaagtag accaccaatc aaaaccgcca ccattaagaa aggcagtaag aaaccatgaa
     3121 gctgaaaact ttagtgattg gtggttcggg attattcttg atggtcttct cactgcttct
     3181 gtttgtcgcc attttatttt cagatgaaca agatggcggt ttttccaaca ttcactatgg
     3241 cggtgtggat gtttccgcag aagtgctggc tcataagcct atggtagaaa aagttgccaa
     3301 agaatatgga attgaagaat acgtcaatat acttcttgcg attatacagg tggaatcggg
     3361 tggtacagca gaagatgtta tgcagtcctc ggaatccctc ggtcttccac ctaattcatt
     3421 gagtacagaa gaatccatta agcaaggtgt gaagtatttc agtgagttat tagccagtag
     3481 cgaaaggctt ggtgtagatt tagaatcggt tatccagtcc tacaattatg gtggtggttt
     3541 cttaggatat gtggctaatc gtgggaataa atataccttt gaactggctc aaagtttctc
     3601 aaaagagtat tcgggtgacg aaaaagtgtc ttaccccaat cccatagcca tacctatcaa
     3661 tgggggctgg cgatacaatt acgggaatat gttttatgtg caactggtaa cgcagtatct
     3721 tgtcacaaca gagtttgatg atgatacagt acaagccatc atggacgaag ctctgaaatt
     3781 tgaaggctgg aagtatgtct atggcggagc ttccccgact acctcttttg attgtagcgg
     3841 actgacacaa tggacgtatg gaaaagctgg aatcaactta ccacgaacag cacaacagca
     3901 atatgatgta acccggcata tcccattatt ggaagcaaaa gccggcgatt tggttttctt
     3961 tcattctacc tataacgctg gctcttatat tactcatatc gggatttatt taggcgataa
     4021 ccgtatgttt catgcaggcg accctatcgg ttatgccgac ttaacaagct cttactggca
     4081 acagcattta gtgggagcag gacgaatcaa acaataagaa aggacgattc aatgatgaaa
     4141 ttcggaaaaa atcagaatac agaaaaacag acaccaaagg aaaagaaacc tcgtgtctat
     4201 aaggtcaatc ctcgtaaaaa ggttgtgatt gtcctatggg tacttttagg acttagtttc
     4261 agctttgcga tattcaagca ctttacagcc attgataccc ataccattca tgaaaccacc
     4321 atcatagaaa aggaatatgt cgatactcat catgtggaaa attttgtaga gaactttgcg
     4381 aaagtctact attcatggga actaaacgac aagtccattg ataatcgcat agaaaacctg
     4441 aaagcatatc tgacagagga acttcaagct ctgaatgtcg atacagttcg taaagatatt
     4501 cctgtatcgt cttctgtaaa aggatttcag atatggacgg tagaagcaga tggcgacaat
     4561 caatttgatg taacctacag tgtagaccaa ctcattacag agggggaaaa tacaaagacc
     4621 gtccactctg cttatatagt gagtgtctat gtagacagta ctggaaatat ggtcctcatt
     4681 aagaatccga ccattactag cataccaaag aaatcagact ataaaccaaa agctattgaa
     4741 agtgagggta cggttgattc cattacaacc aatgaaatca atgagttttt aacgacgttc
     4801 ttcaagctct atcctacagc gacagccagt gaactttcct actatgtgaa tgacgggata
     4861 ttaaaaccaa tcggaaaaga gtacatcttt caagaactgg taaatcctat ttacaatcgt
     4921 aaggataatc aagtcacggt atcgctgaca gtggagtata tcgaccagca gaccaaagca
     4981 acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg gaagattata
     5041 gaataacaaa tattggtaca ttattacagc tattttgtaa tcacgtactc tctttgataa
     5101 aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt atctatttaa
     5161 aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagtggga
     5221 acaaataatt ggatgtcctt ttttaggagg gcttagtttt ttgcacccag tttaagaata
     5281 cctttatcat gtgattctaa agtatccgga gaatatctgt atgctttgta tgcctatggt
     5341 tatgcataaa aatcccagtg ataagagtat ttatcactgg gatttttatg cccttttggg
     5401 cttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag ctcatgttga
     5461 tgc
//
LOCUS       DACEZN010000164         5411 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEZN010000164 DACEZN010000000
VERSION     DACEZN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.164"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..641
                     /locus_tag="IXH60_002446"
     CDS             <1..641
                     /locus_tag="IXH60_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ1991334.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(727..1821)
                     /locus_tag="IXH60_002447"
     CDS             complement(727..1821)
                     /locus_tag="IXH60_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1991335.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            2010..2771
                     /locus_tag="IXH60_002448"
     CDS             2010..2771
                     /locus_tag="IXH60_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1991336.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2886..3326)
                     /gene="fabZ"
                     /locus_tag="IXH60_002449"
     CDS             complement(2886..3326)
                     /gene="fabZ"
                     /locus_tag="IXH60_002449"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1991337.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3546..4301
                     /gene="fabI"
                     /locus_tag="IXH60_002450"
     CDS             3546..4301
                     /gene="fabI"
                     /locus_tag="IXH60_002450"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1991338.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(4411..>5411)
                     /locus_tag="IXH60_002451"
     CDS             complement(4411..>5411)
                     /locus_tag="IXH60_002451"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1991339.1"
                     /translation="NNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEGSPALH
                     YGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVKQVVKA
                     NEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLKPTNFI
                     VSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEVGAANF
                     FGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAEAGACG
                     TAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIEVVE"
BASE COUNT         1694 a         1088 c          949 g         1680 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagagaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata acctataaaa
      661 aaacaaaata aaaaagcaga aataactggc tatttctagc ggattatttc tgcaataact
      721 aattatctat tttttatttt caattaattt aaagcttgac catggtttca aaaagtcaag
      781 gaggaataat tcgtcatcgg agattcttcc tacaacattg acacgtccgt cgtttttcat
      841 atccttcaag gcgatttgtg tttctccttt gtattgaccg tacccttcat tgtcaatcag
      901 tacatcccca tgatgcatat ctcgcgtatt atgaggcggg aaatctttat ctttgtagta
      961 tacacgagtc atcgttgaac gaaggatata ttctgagcga tcccctcgat aactatgaag
     1021 gttatcaaac aagcagattc gttcgttttc tgtaatacct tcttccgtat ctactttcaa
     1081 tgttggataa tccgcgttaa atgcttctgc catttctttc aattcttctt cacttgcgta
     1141 cgcatttcca atagaaatat catcaatcaa tcctgtaagg attaaatgct tcacttgtgt
     1201 cgcaatttca agatctcggt ggtcttccag cgtgcacaag ccatcttgcg taggccaagg
     1261 gccaaactca gcagattgcg aattgacgaa tgccatcgta ttcaaattat attttctgaa
     1321 tttttctgaa caaaaaacaa agtgttcgta tcctaaacca gagtatcgat gaggataaaa
     1381 attatgagaa cctaataaat tatccgtgtt tggcgaataa ctcatgatgt tgtcaacgta
     1441 attcgtgccc gaactcatat tgatctcgat tttgatacca aaaggattgc gcgtcatttt
     1501 tgcttcttct gctcctgtga atccgatatc taagcggacc ccgtaggcac ccatcttatg
     1561 gaaaaaagat aagtcatcgt aagaaatctc caactgttca aataacgctg gattgatatc
     1621 aaccattact tccatgccta ggctattcgc gtaatcaact acttctttaa attctgaaag
     1681 aactttttct ttatcatcat tgatctgcag caaactggta aaaacccgtt tatacccata
     1741 cttatgagct aaatccaagt acgctttgtc cttttcaaat gttgaccgtt ctggataaat
     1801 cgagattcct aattttccca tattaccatt tcctcctgca tcttttcgtt tttgtatcca
     1861 cttacattat agcaaacaca aagtaaatgg tctacacctt ttctttttct tttaaaataa
     1921 atgataaatt ccaataattt aagtaggtat tttccctcta ttttgttatc attaaagata
     1981 acaaaatagt aaattggaaa ggaacagtta tgaagaaaaa taaagttatt ttaagtatta
     2041 gtttagcagc agcattgctg tttgctgctg gttgttctgc aaacaacacg acttcttcta
     2101 gttcgtctac agaaagttca aaaagtacat ctactgcgga aagcacgact gccagtagcg
     2161 tcgatttgaa cgcattggaa ttaccacaat tgacgaaaga tgtcgccgaa aatgaagatc
     2221 tggtcgaaat ggtgacgaat aaaggcacaa tcaaaatcaa attatttccc gaacacgcac
     2281 caaaagcggt tgaaaacttt atgacacatg caaaagacgg ttactacaac ggactgactt
     2341 tccatcgcgt catcaaagat ttcatgatcc aaggtggcga tcctaaaggc gatggcactg
     2401 gaggggaaag catttgggga gaaggatttg aaacagagat caacaaccac ctttataata
     2461 taagaggtgc gttatctatg gctcgctcac aagatcctaa cagtaacggc agccagttct
     2521 ttatcgttca aaatacagat gatatgcatg atggtctttt aaaagacgac tatcctcaag
     2581 caatcatcga cgcttataaa aacggcggtt atccttcact agatggccat tatactgtct
     2641 tcggccaagt gattgaagga atggatgtcg tggatgctat tgcaaatatc gaaacagata
     2701 gttcagacaa accaaaagaa gaagtaaaaa tcgataaaat caatatactg caagaagcaa
     2761 aacaaaaata aaaaaataac gtctgaatcc tgcatagatg acaacgtatg ctaggattca
     2821 ggcgtcttct ttttacataa aaagcaagta agcttccttt tgttttgcta tatcaggttt
     2881 tatagtcaag cattttggtt gacgatgaac gtgaactcgc actcacagac ttttttatca
     2941 tctacatatg ctgcagataa cgctgtaccg actgatccct tcattttggt gatttcaaaa
     3001 tgacatttca gcacatctcc tggaacaact ttttgacgga atttggcctt attgatccca
     3061 ccgatataag ctctttttcc tagaaactct tctgatttta ggataagtat cgaacctgct
     3121 tgtgccagtg tttctagaat caacgcaccc ggcatcactg gattgtcagg aaagtgacct
     3181 tggaaaaaat cttcattgat tgttacattt tttgtcgcaa cgatcttttt tcctggttct
     3241 aaagtatcta cataatcaat ataacaaata ggataacgat taggaatcaa ttccattact
     3301 tcagtagcag ataatacgta agacataatt aaactccttc tttcaggcaa ttgccatttt
     3361 cattactttg actatcaaag tatcatattc ctaaatattt agcaatcttt ttttaattta
     3421 agaaaaaata gtttgacaat caaaacattt tgactaataa ttaagaaatc aagaagttat
     3481 tttagctatt attctctatt tttttacgac tatcaaattt ttatcagaaa caaaggagag
     3541 tcattatgtc atttctagaa ggaaaaaaaa tcgtcattat gggcgtcgct aacaaaaaaa
     3601 gcattgcttg gggttgtgct gaagcaatgt tgaaacaagg ggcagaaatt atctatactt
     3661 accaaaatga acgaatgaaa aaatcattag tcaaattggt cggcgaagaa gcattcactg
     3721 tagaatgtga tgtttctagc gacgaaagca tcgaaaaagc aatgaatgcc atcggtgaat
     3781 atgctgggga gatccacggc cttgtccatg cagttgctta cgccaacaaa gaagaattgt
     3841 ccggaaatgt gtctgatatt tccagagacg gctatcaatt agctcaagat atcagcagct
     3901 actctttaat tgctgtatca aaatacgcac aaccgtttct tgcaccaaac agcgggatcg
     3961 ttagcatgac ttatctaggt tcagaacgcg caatcccgaa ttataacatg atgggtattg
     4021 cgaaagcttc tcttgaagct gctatccgtt atttggcagc tgagttctcg cctaaaggaa
     4081 ttcgggtcaa tggaatctca gctggggcaa tcaaaacatt agctgtcaca ggtgttaaag
     4141 actatcaaaa actgatccag ttatctgaat ctcgtacgcc tgatcagcaa ggtgtgacga
     4201 tcgaagaagt cggtaatgct tgtgctttct taatcagccc actttccagc ggaatcatcg
     4261 gtgatattat ctacgttgac aaaggtgtcc atctttcata aacttttgtt gcattcttta
     4321 aagtaaaaca tataaaaatc aggaaaaaac tttttaaagt ctcttcctga ttttttcttt
     4381 ttacgttttt ttcaactcat tactttgaaa ttattccacg acttcaatcc agccttctgg
     4441 tgcttctatg tctccaaatt gaatacctgt caattcatca tacaaccgtt ttgttactgg
     4501 tcctacttcc gtttcactat aaaatacatg aagattgtta ttgtgataaa tcccaccgat
     4561 tggagaaata acagccgctg taccacatgc accagcttct gcaaattgat ctagatcatc
     4621 aatgaaaata tctccttcaa ttgcttgcag tcctagtcgt tcttcagcta actgcaacaa
     4681 ggaatatttc gtgatacttg gtaagataga gggcgattgc ggagtgatga attttccgtc
     4741 ttttgtaatt ccaaagaaat ttgctgcacc tacttcttcg attttcgtat gtgtggctgg
     4801 atcaagataa acacaatctg agtaatccag ttcttttgct tctgttcctg gaagtaaact
     4861 cgctgcgtaa tttccgccga cttttgctgc accagtcccc ttaggagcag cacgatcgta
     4921 atcagataca ataaaattcg ttggttttag tccacctttg aaatacgctc ctactggcgt
     4981 acagaagacg acaaataaat attctggagc tggatggaca ccaatgtttt caccgacccc
     5041 aatcagtaaa gggcgcaagt ataatgtagc gcctgttcca tagggcggca caaaatcttc
     5101 atttgctttt accacttgct tgacagcatc aacgaacatc ttttcaggga ccattggcat
     5161 cagcaatcgt ctagcacttt gattcatccg tcgactattt tgttcaggtc gaaagagatt
     5221 gaccttgccg tcttttcgac gataagcttt tagaccttca aaacattgct gaccataatg
     5281 aagcgctgga gagccttcat gaatcgtcag gtaattatct tctgtcaatt ctccttcttc
     5341 ccattgtccg tctttccatt tggcaataaa acgccaagga gttttgatat aagaaaaacc
     5401 tagattgttc c
//
LOCUS       DACEZN010000165         5350 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEZN010000165 DACEZN010000000
VERSION     DACEZN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.165"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            68..1945
                     /gene="tdc"
                     /locus_tag="IXH60_002452"
     CDS             68..1945
                     /gene="tdc"
                     /locus_tag="IXH60_002452"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1991340.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2188..3609
                     /locus_tag="IXH60_002453"
     CDS             2188..3609
                     /locus_tag="IXH60_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1991341.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            3718..5085
                     /gene="nhaC"
                     /locus_tag="IXH60_002454"
     CDS             3718..5085
                     /gene="nhaC"
                     /locus_tag="IXH60_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1991342.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         1601 a          977 c         1144 g         1628 t
ORIGIN      
        1 agaatagaag aacaggtgta atgtagggct cgacaaaatt taaatatttt tttaggaggg
       61 tttcactatg agtgaatcat tgtcgaaaga tctaaaccta aatgccctat tcattgggga
      121 caaagctgag aacggccaaa tctataaagc tttgttgaat gaattggtgg atgaacattt
      181 aggctggcgt caaaactaca tgcctcaaga catgcctatt attacgccag aagaaaaaag
      241 cagtgctagt tttgaacaca ctgtgaataa aacaaaggat gttctatcag aaatttcagc
      301 gcggatgcgt acccattccg ttccttggca taatgccgga cgttactggg gtcatatgaa
      361 ctctgaaaca ttgatgccat ctttactagc gtacaatttt gctatgctat ggaatggtaa
      421 taacgttgcc tatgaatctt ctcctgcaac aagccaaatg gaagaagaag taggaatgga
      481 atttgcaaaa ttaatgagct ataaagatgg ctggggccac atcgttgctg atggttcttt
      541 agcaaactta gaaggacttt ggtatgcccg caacatcaaa tcattgccgc ttgcaatgaa
      601 agaagtgaca cctgaactag ttgctggaaa aagcgattgg gaattaatga acttgtcaac
      661 agaagaaatc atgaatctgt tagacagcgt cccagaaaaa atcgacgaaa tcaaagctca
      721 ctcagcacgt agcggaaaac atttagaaaa attaggaaaa tggttggttc cacaaactaa
      781 acactattca tggctaaaag ctgccgacat catcggtatc ggtttagacc aagtgattcc
      841 agtgccagtt gaccacaact atcgcatgga catcaacgaa cttgaaaaaa tcgttcgtgg
      901 tctagcagct gaaaaaacac caatcctagg tgtagtcggt gttgtaggat caacagaaga
      961 aggagcaatc gacggtatcg ataaaatcgt tgcattgcgt cgcgtattag aaaaagacgg
     1021 tatttacttc tacttacacg tagatgctgc ttatggcgga tacggccgtg cgattttctt
     1081 agacgaagac aacaacttca ttccatttga agatttaaaa gatgttcatt acaaatacaa
     1141 tgtctttaca gaaaacaaag attatatctt agaagaagta cacagtgcat ataaagcaat
     1201 cgaagaagca gaatctgtaa cgattgaccc acataaaatg ggctatgttc catattctgc
     1261 cggtggtatc gtcatcaaag atatccgcat gagagacgtt atctcttact ttgcgacata
     1321 tgtatttgaa aaaggcgcag atatcccagc attacttggg gcttatatct tggaaggttc
     1381 aaaagcaggg gcaacagcag ccagcgtttg ggcagcacac catgtcttac ctttgaatgt
     1441 cacaggatat ggtaaattaa tgggtgcatc aatcgaaggt gcacatcgtt tctacaactt
     1501 cttgaatgac ttgtcattta aagtaggaga caaagaaatc gaagttcatc cattaactta
     1561 tccagatttc aatatggtag actatgtatt caaagaaaaa ggcaacgatg acttagttgc
     1621 aatgaataaa ttgaaccatg acgtatatga ttattcttca tacgtgaaag gaagcattta
     1681 cggtaatgag ttcttaactt cacatactga ctttgctatc ccagactatg gcaacagccc
     1741 attacaattt gtgaaccaac taggattctc agatgaagaa tggaaccgtg ccggaaaagt
     1801 aactgtattg cgtgccagcg taatgacacc atacatgaac aaagaagaac actttgaaga
     1861 atatgctgaa aaaatcaaag cagctcttca agaaaaattg gaaaaaatct acgcagacca
     1921 attattggca agcgaagcaa aataagcaag gtaatcatgt tagaaaacag cttgaatact
     1981 aactgtttct gacgcatgtt tggatctgaa gagcaagggg agacctaagc tcttcgatcc
     2041 ctttgttttg aggttgtctt tgaagaaacc cttcaagcaa taaactcaaa gaatcccctc
     2101 aaatattcgc tgaattgttt cgtttattgc tgaaactact tcaaagacac cgttatttta
     2161 tccaaaaaat caaggagagt gtcaacggtg agtaaatctg aagtaagtaa aatgggtctt
     2221 gggaccttta tcggtctgac aatggcactg tgtgcaacgg ttcggagtat tcctacgtta
     2281 gccgcagtcg gttggacatt gattttttat tcaatttttg cagttttgtt ttttgccggt
     2341 ccgatatcaa tggtttccgg tgagctttcc acgatgcttc ctcaagaagg cggtcctcag
     2401 ttatgggtaa aaacagcact gggcagcaaa tggggatttg tcgttgcatg gctattatgg
     2461 gtgcaaatgt tcccaggtat ggtaatggtc gcttctactt taggaccatt gttaggaaac
     2521 acatttggta atgtagaact tggcaataat cactggttcg ttttaggttg tattttagtt
     2581 atctactgga tcatcacgat cttgaacttg aaattcgata tggcaaaagt cggcgggaac
     2641 atcggtgtat ggctaggggt atatatccca gtagtcatca tgttcgtttt aggtgttttg
     2701 gcagccttca aagtcggact agtctcaaac ggctatctag gtgacttttc atggtcaaaa
     2761 gctttccctg acttggaaca tattgattca ttgaaatact tagctggtat cacatttatt
     2821 tttgttggga tcgaaatgag ttctgtttac atgcctcgtt tgaaagacgc aacaaaaaat
     2881 tatacaaaag gcgtatttat cgctttgatc ggattagttt tattgaatgt catcaatgcg
     2941 atgcttgtag caaatgtcgt accagatgga aaaatggaat tagcaaatat cacgcagcct
     3001 atcttgattg actgtcaaat cctaggctta ccagaagtga tcggaaatat cttcagcttc
     3061 atggtattta ttggtgtgct tttacaatta tcagcttggg ttactggacc atcaaaaaca
     3121 atcatccaag tagctcgtga aggattcctt ccaccaaaat tcggattcca taaagaaaac
     3181 aaatacggcg tatctagaaa tgtcgttcta acacaatcaa tcgttatttc attatttgct
     3241 ttgctttatg gtgtaatgga tgacgtaagt gctgtattct tgacacttac aaatgctaca
     3301 actgttattt attgtatcgt gtacattttg attgctgttt cattgcttaa aatgcgtaag
     3361 aaacatccag aattcgaacg tccttaccga attggtaaaa acggaaacgg attagcttgg
     3421 gtagtcagct gcatgctgat cttctctatc atcgttgtcg tatttgctac attaggtaca
     3481 gcgacattga gtgatgcatt attagtagca gctatcacag tggtcatgtt cgttattcca
     3541 ttgatcatca atcacttcaa gaaagattct tgggggattg aggtagaaaa aagtttagaa
     3601 gaaaaataaa aaaataagtc tttcagccga cgttttgtcg ctgatttagt aggaggtcct
     3661 gccaagtgaa tagttttttc atggcagggc cttttcattt gaaataggag atcgacgatg
     3721 aataaaaata tttctttaaa agaaagtatc gctgtattga tcgtgttgtt ggcactccta
     3781 ggagtactga tcatcggatt ccagctctcg ccacatatcc cgatccttgc agctttcatg
     3841 tgtttgttgt tttatggcag atttaaaaag ttcacatggg atgagatcca tgacgggatc
     3901 atcaaaggaa tcactccagg gatcattcca atcctgattt ttcttttgat tggtgtctta
     3961 gttgcttcat ggattttatc tggaaccatc cctacgatta tggtttatgg atttaaactt
     4021 gtttctgtgc gtttcttttt accgacagca tttcttgttt gtgcaattgt cggagtgact
     4081 gtcggcagtt catttacaac gatttcgacg attggaattg cttttttggg gatcggtcat
     4141 ttacttggat tcaatgatgc aatgactaca ggcgcagtcg tttccggtgc ctttttaggg
     4201 aataactgct cgcctttatc agatacgacc aacttggcag cgggtattgg aggagttaat
     4261 ctattcgaac atatcgctgg tatgcgttat acggatttcc cagcattcgt cctttcgttg
     4321 attttttata cgatattagg acataacagt cattcagccg atctagctag tatcaatgaa
     4381 atgatccaaa acatgaaagc gagcttttgg atctctcctt ggacattgat tccactagtt
     4441 attttatttg ggggggcaat caaaaaaatt ccggctatcc ctacattact agcgggatcg
     4501 acagctgctc tcgttctttc ttttatccat gagaaaagtt tgacggtcgg aaaagcagca
     4561 gacatcttga tgaatggtta tgtcgcaaat acacctgatc caaagctaga tgaactgctt
     4621 tctcgaggcg ggatcatgag tatgttaggt tctgctagtt tgattttact tgcattggca
     4681 ttaggtgggt tactgatcaa atatctgatc gttgatacca tcgttcaaga attaaaagaa
     4741 aagatggacc gaccttctcg tttagtcggg tttactgcct tgagctgtat cggaatcaat
     4801 ctgatcgttg gagaacagta tctgtccatc attcttccag gagaaacatt caagcgttct
     4861 tttgatgacg ttggcttatc aaaaacctat ttgacacgaa cactagcgga ttcaggtgcg
     4921 accgtcaatt ctctcgtacc gtggggagtc agcggcacat tcattatggg aacgatgaaa
     4981 gttagcgcgt tgcattactt gccattcgcc tttttctcga ttcttgcacc tgtctttaca
     5041 atattaggcg gttttttatt gaatcgtcga cgtgtaaaaa attgaaggaa ggaaaaaaga
     5101 ggctgggaca gaagcgttta actccaagaa ataagaagaa actcaagaaa attgcttttt
     5161 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
     5221 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttacagat
     5281 aaacaatgtg ttagaagtag tatcttcttt tacataaaaa gctgaccata ggataaatgg
     5341 tcagcgtatt
//
LOCUS       DACEZN010000166         5345 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEZN010000166 DACEZN010000000
VERSION     DACEZN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.166"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..939
                     /locus_tag="IXH60_002455"
     CDS             <1..939
                     /locus_tag="IXH60_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1991343.1"
                     /translation="EWKETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLA
                     IDILTFLTGISSRDSEKFPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHI
                     ESFVDYDFAHLLFSDGSVTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAM
                     LIVISTFALDFIHSDRSIFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVT
                     QNADDLLDEKMKNNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCL
                     VSDIYGRVGVMQFHPIFEELLHAFDTRPPVRNEVEE"
     gene            936..2963
                     /locus_tag="IXH60_002456"
     CDS             936..2963
                     /locus_tag="IXH60_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991344.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            2960..3982
                     /locus_tag="IXH60_002457"
     CDS             2960..3982
                     /locus_tag="IXH60_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1991345.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            3999..4928
                     /locus_tag="IXH60_002458"
     CDS             3999..4928
                     /locus_tag="IXH60_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991346.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            5173..5289
                     /locus_tag="IXH60_002459"
     CDS             5173..5289
                     /locus_tag="IXH60_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1991347.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1755 a          948 c         1091 g         1551 t
ORIGIN      
        1 gaatggaaag aaaccttacc tgatattgcc catgaaatca atattgttaa tctcacgagt
       61 gagaacgaca ataaagggtt acttgaccct tatgtgattt taaaactccc gaaagattca
      121 gaaagccttg ccattgatat tctcacgttc ttaacaggta tttccagccg agatagcgag
      181 aagtttcctg tcctgcgtaa agctattcgg agtgtaactc aaagcaaaca acgagggttg
      241 ttattggtga ttgatgaatt gcgaaaagaa gatacgccca tcagcaatag tattgctgac
      301 catatcgaaa gctttgtcga ttatgatttt gcccatctgt tattcagtga tggcagtgtc
      361 acccaatcca ttagcttgga aaaacaactc aatattattc aagtggcaga tttggtctta
      421 cccgattcca atagctcttt tgaagaatac actaccatgg aattattaag tgtggcaatg
      481 ctgattgtca ttagtacctt tgctttagac tttatccact ctgaccgttc catttttaaa
      541 attgttgacc ttgatgaggc ttggagtttt ttacaagtgg cacaaggaaa agctctatct
      601 atgcgattgg ttcgagcagg acgtgccatg aatgcagggg tctatttcgt tactcaaaac
      661 gcggacgatt tactagatga aaagatgaag aataatattg gcttgaaatt tgcctttcgt
      721 tccactgacc tagtggaaat caaaaagacc ttagaatttt ttggtgtgga caaggaagat
      781 gaaaacaatc agaaacgctt acgtgaatta gaaaatggac aatgtttagt ttcagacatc
      841 tatggtcgtg taggggttat gcagtttcac ccgatttttg aagagttgct tcatgccttt
      901 gataccagac cgcctgtgcg aaatgaggtg gaagaatgac acaaaaacaa aagaaatttc
      961 ttcactatag ttggataacg ctcctttgtg tgggcgttat tttactttta ttaggcacgc
     1021 tgggcaatgc agtacaagcg acaggcttag ttgatgaaac cattgataca agcaatgaat
     1081 actccaagta tggtttgaat caatatcagc ttgattatta tgtggacaat agctggggct
     1141 ggcttccatg gaattggagt gatggcatag gacaatcggt gatgtatgga ttgtatgcca
     1201 ttactaattt tatttggaca atttctcttt atctatcgaa cgccacaggt tatttggtga
     1261 aagaagccta tgccttagat ttcattagtc aaacatccca agcaattggc gaaaacatgc
     1321 agacgattgc aggcattaca ccgaatggct tttccagttc agggttttat gtcggtttcc
     1381 tccttctttt tattctcatt gttggaatct atgttgccta tacaggattg gttaagcgag
     1441 aaacgacgaa agtgatacga gctgttttaa actttgtcgt ggtgtttatt ctatcggctt
     1501 cctttattgc gtatgcaccc gattatattg cgaaaatcaa tgatttttcc aaagatgtca
     1561 gtaccgccag tctgaatatt gggacaaaaa ttgttcttcc taacacggat agtcaaggga
     1621 aagacagtgt ggacatgata cgtgacagct tattctctgt tcaagtaaag caaccatggc
     1681 tattgcttca atatggctcc actgatgtag aagcgttagg ggaagaacgt gtggaaagtt
     1741 tattgtctac cagtccagat acaaacaatg gtgaagatag agaaaatgta gtgattgaag
     1801 aaattgaaga tcatgacaat acctatttaa ccctaccgaa gacgatttcc agattaggaa
     1861 cagtcttctt tttgtttgta ttcaatattg gtatctcgat ttttgttttc cttctaacag
     1921 gaatcatgat tttttcgcaa gtgctattta ttattttcgc tatgtttttg ccaatcagtt
     1981 tcctattaag tatgttgccg agttttgatg gcatgggcag gcatgccatt accaagttat
     2041 ttaatgtgat tatgacaagg gctggtatta ccttgattat taccgttgcc tttagtattt
     2101 ccactatgct ttattcgctg tcagcaagtt ctcccttttt catgattatg tttttacaaa
     2161 ttgtgacctt tgcagggatt tattttaaac tgggcgattt aatgagccta ttcactttac
     2221 aaagcaacga ctctcaaaac atgggcagac aagtgtttcg cagacctcgt caaatgatga
     2281 atcgacaaac cagaagattt caacgcagta tgagtaagat ttttcgtgga aaagcagggt
     2341 caacgaagca aaagacaatg acaacaaagc agaatcaaac caataaagct aaccatactc
     2401 gtcaaaatga acggactaca ccaaagaaag aaacagcact ttccaatgtg aaaaagcaac
     2461 aaggcggtat cgggcaacga ttaggtgaaa agacagccca tgtattggac acgaaaaatc
     2521 gtctagtcaa taaaagaaag caggcaaaac aacagataca ggcgacacca acccatgtca
     2581 aatatgccct tcatcaagga aaagagaatg tcactcataa tgtaacagac tttaaacaat
     2641 ctatggcaga taccaaagct caacgcaaac aacagcgtat gaaccagcag gagcaaaaac
     2701 gaaagaacat tgctcaaaag cgtttggagc tggataaagc aaaagaaaaa agacgaaaag
     2761 aaaacccatc atctcctgtt cagaataacc agcagcatgt tcgcaagcgt cagcaaaccg
     2821 tcacgcctgt tttgtcacaa aacactaaac gtgaaaatca tcagcgacca ttgaaaaaag
     2881 agcaaaaaca aaagacacca ccgaagcaat tcaatcagag gaagctaaat aaaagaacgg
     2941 ataagcgagg acaacaaaca tgaagctaaa aataacagtg atttgtagtg tggtcttctc
     3001 tctcttcttt tttctcttat tattcctagt cattttcttt gcagatgata cggacagtgg
     3061 cgaaaacaac aaggattctt ctattccaca aggcggtgtg accgtttcac cagaagtgtt
     3121 ggcacaccgt ccgttgattg aaaaatatgg taaggaatat gggattgaag actatgtttc
     3181 ttatatctta gccattatgc aagtggaatc gggcggtaca gcagaagatg ttatgcaaag
     3241 ttccgaatct ttgggtttac cgcccaacag tttgagtacc gaagagtcca tcaaacaagg
     3301 ggttaaatat ttcagtgaat tattaaccag tgcagaacaa caaggagtgg atatagattc
     3361 tgttatccaa tcttataatt atggcggtgg ctttttaaat tatgtgagga gtcatgggaa
     3421 aaaatatacc tatgaattag ccgaacagtt ttctaaagag aagtcgggtg gtcaaaaagc
     3481 agactatccc aatcctatag ccatacctgt gaatggtggt tggcgatata actatggaaa
     3541 tcagttttat gttcagttgg tttcacaata cctaaccgac acttccccaa cagaatttga
     3601 tgatgaaacc gtgcaagtca ttatggacga agccttgaaa tacgaagggt ttccctatgt
     3661 ctttggaggg gcgtcgccta ccacctcttt tgattgtagt ggcttaatac aatgggtgta
     3721 tgacaaagct ggcatttctc tccctcgtgt cgcccaagat cagtatgacg ctacgcaaga
     3781 aatctctatg gaagaagccc aagcaggcga ccttatcttc tttcattcaa cctataatgc
     3841 aggcacatat gtgactcatg tggcgatata tttagaggga aatcgctttt atcatgcagg
     3901 cgacccaatc ggttatggcg acttaagcag tcgttattgg caagaccatc tgattggggc
     3961 tagacgtgtt attcataact aagaaaggaa tgaatcaaat gattataaaa atcgagcgaa
     4021 aagaaaagaa ggagaaacca aagaaatcta aaaaaccgag caagcaaaga aaagttccga
     4081 tgattaaagt tggcacgcat aagaaattaa cctttgtttt atgggtttta ttgattggta
     4141 gtgtcggatt tggaatctat aaaaatttta ctgccattga cactcatact gtccgtgaaa
     4201 cagaaatcat taaacagcag attgtcgata ccaaccaagt agaaagtttt gtgaaatcct
     4261 ttgcgaaaga ctacttttcg tggcaacaat cccaagaagc cattgataaa cgaaatgaaa
     4321 aattgaccca ttatttgacg gaagaattgc aggtattaaa tgaagaaatg attcgtaagg
     4381 atattcctac tagttctagt gtgaacgata tacaggtgtg gcaggtttct caagtgaatg
     4441 agaacacctt tgaggtactg ttttctgttg aacaagttat taccgaagac aaagacaagg
     4501 aaactatatc gtctagcttt catgtggttg tccatataga tgaatcagat aatatggtta
     4561 ttatcaaaaa tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa cccaaacagc
     4621 tagaaagtga ccatacagta gatacagaaa cgatggacga aatcactagc tttttagaaa
     4681 cgttcttcca gctctatcct accgccacag aaaaagaatt gacttactat gtaagtaatc
     4741 atgtgttacc tatgattaac aaggaatatg ttttcgagga attggtgaat cctatcttta
     4801 ccagaaaaga taaccaagta atcgtgaatg tcgctgtgaa atatttagat caagaaacaa
     4861 aggcaaccca aatctcgcaa tttgagttga tattagaaaa acaagataat tggaagattg
     4921 taaaataaca aatattggta catgattaca gatactttgt aatcatgtac tctttttgat
     4981 aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa gtgactattt
     5041 aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagcgg
     5101 gaacaaataa ttagatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa
     5161 tacctttatc atgtgattct aaagtatcca gagaatatct gtatgctttg tatacctatg
     5221 gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg
     5281 ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt
     5341 gatgc
//
LOCUS       DACEZN010000167         5333 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEZN010000167 DACEZN010000000
VERSION     DACEZN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.167"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..334)
                     /locus_tag="IXH60_002460"
     CDS             complement(<1..334)
                     /locus_tag="IXH60_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991348.1"
                     /translation="MHVRCGAGENLEITSKGYLSLFTSDFLAGLGFGATQMLGELEGI
                     YFGYNVDTGRNVYLKPALASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFG
                     GQAVIVDPK"
     gene            complement(374..2176)
                     /gene="ltrA"
                     /locus_tag="IXH60_002461"
     CDS             complement(374..2176)
                     /gene="ltrA"
                     /locus_tag="IXH60_002461"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1991349.1"
                     /translation="MSTELRYYEYYQMQETFDWLYERSSKQATKGIDLYKIIMSEENI
                     LLAYRIIKSNTGSKTSGIDNQTISDFKFQNKQKFVNDIRKTIENYQPQPVRRVNIPKS
                     NGKTRPLGIPTMKDRLIQQMFKQVLEPICEAKFFKHSYGFRPNRSTEHAIARCNFIAH
                     KCKCHHVVDVDIKGFFDNVHHGKLIKQLYTIGIKDRRVLAIISKMLKVTVAGQGTSTS
                     GVPQGGVLSTLLSNVVLNDLDWWIANQWETFQTKRKYSSVDNMHHALVKSKLKKMHIV
                     RYCDDFKIFTTNHKHAVKIYHAVKGYIKQRLNLDISPDKSKITNLRRNYSEFLGFELK
                     VIKQKKQQYVTVSRVSKKSKSRIKKEIREKIKAIQKSPTRKTVNQYNQYVRGIQNYYQ
                     KATLINHDFSSIYYSCLPALYNRLKPFGKYEIPRSPPITYKKFYPLTKRTFCVRKNYL
                     FPLDSVKWKRLEFFKPHINNYTQDGRLAKLRKLKPSVYSELVTLSTMETDESLEYLDN
                     RLSRYSMQNGKCSVTGIFLRTQDVHCHHIIPRKFGGTDEFDNLVIIHKWVHRLIHAKT
                     KETIKEYLQLLQLTNKQMKKLNKYRLECNLTEFH"
     gene            complement(2884..4113)
                     /locus_tag="IXH60_002462"
                     /pseudo
     CDS             complement(2884..4113)
                     /locus_tag="IXH60_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            complement(4100..4489)
                     /locus_tag="IXH60_002463"
     CDS             complement(4100..4489)
                     /locus_tag="IXH60_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991350.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHMPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(4572..5297)
                     /locus_tag="IXH60_002464"
     CDS             complement(4572..5297)
                     /locus_tag="IXH60_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991351.1"
                     /translation="MLEVMIIMNFDFDLKSFIDIFVPVLTSFAVCFITLFFTNKQHKK
                     AMKQQERQHEESLDLSKQQYADEKQRNEEQDRLSYYPYLKLIPKISEHRFAGKMVKSD
                     ENDVFKLPFELINEGLGMAFSIDFVYLEETINTNDHEVNLLSLLSAQKHIDDSLDALG
                     VSAPIDTDILRVGKKTEFELYWMKYNNEVMSIPKYSVSKWEIKIRFSDFQDRIYQQRY
                     TFITSAGYQEIHRLWSDKPQLID"
BASE COUNT         1540 a         1025 c          868 g         1900 t
ORIGIN      
        1 ctttgggatc aacaatcacc gcttgaccgc caaacaggac agcatagtaa actaaaagat
       61 tattactaaa ggatttccca ccaccaagtg aaccaagaaa agcggcggcc agtgcattgg
      121 tgacggaacc tttgacccct tgtgaagcca atgccggttt aagataaaca ttgcgtccag
      181 tatccacatt gtagccaaaa taaattccct ccaattctcc taacatttgg gttgccccaa
      241 agccaagccc agcaagaaaa tcagaagtaa atagcgatag gtaacccttt gaagttatct
      301 ctaggttttc ccccgctcca caccgtacgt gcaactttca tcgcatacgg cgttccaact
      361 tgttcgtatt tctttagtga aattcagtta aattacattc taaacgatat ttgtttaact
      421 tcttcatttg cttatttgtt agttgaagaa gttgtagata ctctttaatc gtttcttttg
      481 ttttggcatg gatgagtctg tgtacccatt tatggataat aactaggtta tcaaactcat
      541 ctgtaccccc aaatttacga ggaataatat gatgacaatg aacatcttgc gttcttagaa
      601 aaatccctgt aacggaacat tttccatttt gcatagaata tcttgacagt cgattgtcta
      661 agtattcaag actttcatct gtttccatcg tactaagggt aaccaattca gaataaaccg
      721 aaggttttaa tttcctcagt ttagccaatc gtccatcttg cgtatagttg ttgatgtgag
      781 gtttgaaaaa ttctaacctt ttccatttca cgctatctaa tggaaataaa taattcttcc
      841 taacacaaaa cgttcttttt gtgagaggat aaaacttttt atacgtaatg ggtgggcttc
      901 tagggatttc atattttccg aatggtttta aacgattata tagtgctggt aagcaggaat
      961 agtagataga agaaaagtca tggttaatca aggtggcttt ttgataataa ttctgaatac
     1021 ctcgtacata ctggttgtat tggttaacag tttttctagt aggagatttc tgaattgctt
     1081 ttatcttttc tctgatttcc ttcttaattc tactttttga tttctttgag actctcgaaa
     1141 cagtaacata ttgttgtttc ttttgtttta tgacctttag ctcgaatcct agaaattctg
     1201 aataatttct tctaagattt gtaatttttg atttgtctgg tgaaatatct aggttcaacc
     1261 gttgtttaat atatcccttg accgcatgat agatttttac tgcatgttta tgatttgttg
     1321 taaagatttt aaaatcatca caataacgta cgatgtgcat tttctttaat ttcgacttta
     1381 ccaaagcatg gtgcatatta tctacactgc tatattttct ttttgtttga aatgtttccc
     1441 attgattggc aatccaccaa tctaagtcat ttagaacaac attcgataat aatgtagata
     1501 acacgccacc ttgtgggacg ccagaagtgg aagtgccttg tcctgctact gtcactttta
     1561 acattttaga gattattgct aatacgcgtc gatcttttat accgatggta taaagctgtt
     1621 tgattaactt tccatgatgg acgttatcaa agaatccctt aatgtcgaca tctaccacat
     1681 gatgacattt gcatttatgt gcaataaaat tacatcttgc tattgcatgt tctgtagagc
     1741 gattaggtcg gaacccatag gaatgtttga aaaattttgc ttcacatatg ggttctaaca
     1801 cttgtttaaa catttgttgt atcaagcggt ctttcatagt gggaatgcct agaggacgag
     1861 ttttcccatt actttttgga atgttgactc gtctaactgg ctgcggctga taattttcaa
     1921 ttgtttttcg aatatcattt acaaactttt gtttgttttg aaatttgaaa tcactgattg
     1981 tttgattatc tatcccagat gttttagaac cagtatttga tttaataatt ctatatgcta
     2041 gtaggatatt ttcctctgac ataattattt tataaaggtc aatacctttt gttgcttgtt
     2101 tgctacttct ttcatagagc caatcaaaag tttcttgcat ttgatagtat tcataatatc
     2161 gtaattctgt actcactgat ggattatttc tcctaacttt aaaattagtc ccatcctctt
     2221 accagatctt gtgagtttca tttaactgta ttcacttttc aaagaacaag tcttggggct
     2281 atccctccac gttgattaga cgcttcacag gtactgtgcc cctactctcg cagaaattaa
     2341 ggattttcga ctattagtct tatatccaac gctccaataa cagttgttga ataagtgttt
     2401 tctgttttcc tcgttccagt tattctagcc atacatctat ccttaggtgc ttactttgag
     2461 cctgaatatt atataaggtc attgttcctt atcccgaatt tcataaatac gactcttact
     2521 tttcggtaat attcgttgct tttgcaaccc aattatcttt cgataaaagt catcctttag
     2581 acccgtacat tcgcaagttc gtcagttccg taggaacatt ctcaccatag atagtttgtg
     2641 gcgtcccggc atatccttta ccatataagt tattacttac ttaggtattg ttctgccgac
     2701 tttacctagc ttcacacgaa tattcactgt tatgaatatt cgcatgtagg agtatcagac
     2761 cagttgcttc aaccaacttg acggttttca tttcaactgt gaaataacta gttgtaatta
     2821 tctttagttc ttgataattc tcccatttca tgcctatgca cttataggga aacgagtcgc
     2881 accatattgg atatagtcat tcatgtatcg cttgctagct ggcaaaaact cttcatgaag
     2941 tcccatcata tcgccaaagg gacgaaccaa ttttacattg gtatcatcat aaaaatctaa
     3001 aacctcatca caccgacgtt ttaattcatc caatgattct gcactcactc gaacaacata
     3061 gcttaatttg tacatggatt ctttggattg atccaacgtt gtttctagtt catccacgga
     3121 gtccaacgca tccaagacat tggaattggt ttcattgtcg gactggtacg catggttgtc
     3181 taagtctttc agttcttttt tcttattgcg aactgtcgcc agtgcctttt tattcgtcac
     3241 gatttctaca ttcatactcg tgtcgattgg gaaagtgaat tgttgttgct ggtaataaaa
     3301 gagttcagaa gatggaaact ccatctcacc gacaatcgta ttaatcgtta gataggccac
     3361 ataagattca tgattttcat gttccatgtg taagtaccga ggcttctctt caatcaaaca
     3421 gcgacttggg cgaagtaggt catagcgctt tatcaatgtt tcagatttta gctttttctt
     3481 agacaactga aattcatatt cttcaaatgg tgttcctttt tctccataaa tgtgttcaat
     3541 caaataagtt agatcactag gtgttactcg tcttactttg aatctacgag ccaatttact
     3601 ttccatcaat ttttcaagct tagtataccg gcgaatttct tcattggata acgaaacaaa
     3661 atcacccatc aaatgatgat tcactccata gacaaactct tgaagtccgg ataaaaaaga
     3721 gttcttgaga cttttcaggt ttacttcttc gtctgtgaca atcagtttga agccaataaa
     3781 aaagcgatag tcaatttgac tatcgccaat catcgatacc aatgcatccg tctgtaagtc
     3841 gattctctgt tttgcgactt ctttcaaacg accagttatc tcttttttcg accgttcctg
     3901 cgtggcacga atgctgcttt ctgtcgcaat ttgtaacgca tgaatcttac cttcacgatt
     3961 ttgcgcaatc aattggcgaa agttatcgtg gacttgatac ttctgttcaa gtgataagaa
     4021 ggagtaattg taagggacta gttcgtagta agcaaagcac tctccctctt ggttaaaaac
     4081 aagattattt tcaatatatt taatcgggta cataattcac actcctcact agggtgattg
     4141 gttcatccca tcgttttttc tgttctttga ctttctttcc tgcaaaagta accttaaatc
     4201 gcaagtgata actaataaag gatcgtaaaa accctaatgg cttctttcca tcaaaggtct
     4261 tttgcgacat aaaccaagtg actgccacag gtatgcccaa gtattttaaa aatgccccct
     4321 ctatcataga tagtggaggc atatgagcaa acacaatgac aaacagcaag gataagacaa
     4381 accaagccat ttgattaaag gttacaggaa aaggcagctg aaagtcattg attgcgtaaa
     4441 tgactttttc aactgcccag atactggtat agctttttat ttttttcatt agtttcactt
     4501 cctaaaaaag cgcctgataa agacgactct gcatgaatat taattttctt tttaaaagtg
     4561 tattccaact ttcaatctat caactgaggt ttatcagacc ataatctgtg gatttcttgg
     4621 tatccagctg aagtaataaa agtatatctt tgttgatata ttctatcttg aaaatcacta
     4681 aaccgtattt ttatttccca tttactcact gaatatttag ggatactcat tacttcgtta
     4741 ttatatttca tccaatataa ttcaaattct gtttttttcc ctactcttag aatatctgta
     4801 tcaataggcg ctgaaactcc taacgcatcc agactatcat caatatgttt ttgagccgaa
     4861 agtaatgata atagatttac ttcatggtcg tttgtgttga tagtttcttc taaatacaca
     4921 aagtcaattg aaaaagccat tcccagacct tcattaataa gctcaaatgg taatttaaaa
     4981 acatcatttt cgtcagactt taccatttta ccagcaaaac ggtgttcaga tattttgggt
     5041 ataagtttta aataagggta ataacttaga cggtcctgct cttcatttct ttgtttttca
     5101 tcagcatatt gttgctttga taaatcaagt gattcctcat gttgtctctc ttgctgtttc
     5161 atcgcttttt tatgttgttt atttgtaaaa aaaagtgtga taaaacatac tgcaaatgaa
     5221 gttagtacag ggacaaatat gtctataaat gatttaaggt caaagtcaaa attcataata
     5281 atcataacct ccagcattat atatttatca ataattatat ccctaaacct ata
//
LOCUS       DACEZN010000168         5312 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEZN010000168 DACEZN010000000
VERSION     DACEZN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.168"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..218
                     /locus_tag="IXH60_002465"
     CDS             <1..218
                     /locus_tag="IXH60_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991352.1"
                     /translation="KLYEELMTTDEIERSYENDKMYIIPPHYHATDELYKDYEKIKIT
                     AYSSDATELMSGQEFIRIAKENNVVTW"
     gene            230..1234
                     /gene="neuB"
                     /locus_tag="IXH60_002466"
     CDS             230..1234
                     /gene="neuB"
                     /locus_tag="IXH60_002466"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ1991353.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1231..2379
                     /gene="neuC"
                     /locus_tag="IXH60_002467"
     CDS             1231..2379
                     /gene="neuC"
                     /locus_tag="IXH60_002467"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ1991354.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            2352..3029
                     /locus_tag="IXH60_002468"
     CDS             2352..3029
                     /locus_tag="IXH60_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1991355.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3019..4161
                     /locus_tag="IXH60_002469"
     CDS             3019..4161
                     /locus_tag="IXH60_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ1991356.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4174..5151
                     /locus_tag="IXH60_002470"
     CDS             4174..5151
                     /locus_tag="IXH60_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1991357.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5151..>5312
                     /locus_tag="IXH60_002471"
     CDS             5151..>5312
                     /locus_tag="IXH60_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1991358.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRD"
BASE COUNT         1977 a          669 c         1025 g         1641 t
ORIGIN      
        1 aaaaattata tgaagagcta atgactacag acgaaattga acgctcatat gaaaatgata
       61 aaatgtatat cattccacca cactatcatg ctactgacga actgtacaaa gattatgaaa
      121 aaattaaaat cactgcatac tcttcagatg caacagaatt aatgagtgga caggaattta
      181 ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg agttttatga tgaaatcaga
      241 agtatatgta attgcagaaa ttggtgtaaa ccataatgga agtctagatt tagctttaga
      301 aagtattgat aaagctaaag aatgtggagc taacgcggtt aaatttcaaa catttaaaac
      361 gaataagcta gtttccaaaa aggctccaat ggcaaaatat cagaaggata atcttaaaga
      421 ggacagttct caatttgaaa tgctaaaaaa attagaatta tctcaatctg attttattag
      481 aataaaagat tattgtatac aaaataaaat tgattttttg tctactcctt ttgatgaaga
      541 aagtgcagac tttctaaaac aaattggtgt acaaggattt aaaattggct caggagattt
      601 aacaaattta ccttttctta gaaaaataga tatgtatggg ttgccagttt tattatctac
      661 aggtatggct gatattcatg aggtcagtga ggctgtagat tgtttcatag atagtccagt
      721 tactatttta cattgtactt caaattatcc ggctagttct gaagatatta atttattggc
      781 gattaacaca ttaaaagaaa tgttcgggat acccgttggg tactccgatc attcattggg
      841 atatgatgtt ggtatttgtg ctgttgcgat gggtgctaag gtaattgaga aacattttac
      901 cttggataag gaactacctg gtccagatca taaagcttct ttagatcctc aggaatttgc
      961 agattttgta aagcatatta gaaacactga aatattttta ggtgatggtg ttaagcgagc
     1021 aatgccttct gaacttagta caaaagaagt atcaagaaaa agcctagtgg tcactgagaa
     1081 tttaaatgtg ggagatattc ttacagagaa atctcttaca ataaaacgtc ctggaaatgg
     1141 aatagctcct aagtttttat atcaatatat tggcaaaaaa gttaaaagac caataagtgc
     1201 agatagtgtt ctgcttgagg aagatgttga atgaaaaaaa tacttatagt aagtggaaca
     1261 cgagctgatt atggaatata taaacctgtt gctgagaatt tactacaaga taattgggat
     1321 gtatcatttg ttgtttctgg aatgcattta tcccataaat atggatttac taaagaattg
     1381 atcttagctg acggatttag aattagtgga gaagttcaaa gtttgttttt agagaacaat
     1441 aagggaaaca tggctcggtc tgtggcatta gagataaatg gtttcacaaa tatatttgaa
     1501 caaactagcc ctgattttgt attattacta ggggatagag gagaaatgtt ggcagctgcg
     1561 atagcttgtt tgtatcttgg aatcaaaaca gcacatattc acggaggcga agtgtctggt
     1621 actgtagatg aatcaataag acatgccata acgaaaatag catctgttca ttttgctgct
     1681 acggaaaaaa gcaaagagcg tttaataaaa atgggggaag attcttggag agtttttccc
     1741 gtcggagctc ctagaataga tactatttta aataatagat tacctagttt tagagatata
     1801 aaagaaaaat atcatctaaa atttgaaaag aaaaaatacg ttttgcaagt atttcatcca
     1861 gttgtaaccg aactagaaaa tatagaaaaa caaattttag ctttgatcaa ttcaatggaa
     1921 caacaatctc aaccaattat ttgcatttta cctaatagtg atgctggtag cgaaattatc
     1981 agaaaaatgt accagaaata tttaaaaacg gatattattt atattacgaa tttagagccg
     2041 gaggaatacc tgactatatt aaaagagtgc aaatttatga tcggaaattc ttcttctggg
     2101 ataattgaag ctgcatcatt caaaatacca gtaataaatt taggaaccag acaaaatgga
     2161 cgagaacaat ctaaaaatat tataaatgca aatctaaatg aaaatgaaat tgaaactgct
     2221 ttgaataaaa tacaagatcc tcagtttata aaaagcttga aaaatgttaa gaacatctac
     2281 ggtgacggca acagttctag aagaataaca aaaacattga atcaaatatt gaatgatggg
     2341 gaagatatta agtggattca aaaacaaata gcttattaaa atatactagt gatccataca
     2401 ttattattgg ggctggcgga cacgcaaaag tcattattga aaccttaaga ttagaaaata
     2461 agttaatata tggatttttt gatgataagc aaaataaaat gcaagatctg aaatatcttg
     2521 ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa aaaaatctat aaatttattg
     2581 tagctattgg tgactgtgta gtaagaaaaa agattataaa aaaattgaat atgccaagtg
     2641 agcaatatgg aatagctgtt catccaagtg ctatagtggc aaaagaaatt tcaattggtt
     2701 atggaactgt tatttttgcg aatgctgtaa ttaacccaga tgctgtaatt ggagagcatg
     2761 ctataataaa cacaggtagc attgttgagc atgattgtag aattaataac tatgttcatt
     2821 tatctccagg tgtatgctta gcgggtggag tacatgtagg tgtaggaact caaataggaa
     2881 ttggttctca atgtatccag atgaagaaaa ttggatcttg gtgtatgata ggcgcaggga
     2941 gcactattgt taaggatatt ccctcacatt ccttagctta tggcaatcca gctaaaatta
     3001 agaaagaagg aattaatttt gaattctaga attttattat caagtcctca tatgggagga
     3061 acagaagaaa aatatgttgc taaggctttt aaagataatt ggatagcgcc attaggtgaa
     3121 aatgtggata aatttgaaga aagtattaca aaatatactg gttctaaggg cggagcttct
     3181 gcgttaagtt caggtacagc tgccatacat cttgccttaa gactgcttaa tgttggaaaa
     3241 aacgatattg tctttgctcc ttcatttact tttatagcta cagtaaatcc tattttatat
     3301 caaggggctg taccagtgtt cattgactca gaacctaata catggaatat gagccctgtt
     3361 gcactagaga gcgcattaga agaagctgaa agaaacaacc aattacctaa ggcaatcatc
     3421 gtagtgaatt tatatggtca aatggctgat tatgaacgaa ttttagctat tgcaaaccaa
     3481 tttaatgttc cagtgataga agatgcagcg gaatcgttag gttctactta taagggaatt
     3541 tcgtcaggct tatttgggac aattgggatc tattcattta acggtaataa gatcatcaca
     3601 acctcaggtg gaggagctct agtgtctagt gataaaaaat atgttgagca ttcaagattt
     3661 ttagcaacac aagcaagaga tcaagcaccg tattatcaac atagcgaaat aggttataat
     3721 taccgtctaa gcaatatttc tgccggtatc ggacgtggac aaatggaagt attgaatgat
     3781 agagtagcta aacgaagaga aattttcaat cgatataagg aagctttttc tggaattgat
     3841 agaattgaaa tgatgccaga agtggatggc atggtgccaa atcgttggtt gtctacttta
     3901 acgatcaatg attataattc tattgatcaa gtagttaacg taataaataa actaaatgaa
     3961 aaaaacattg aaacaagagc tttgtggaaa ccactacatt tgcaaccagt atttcaaggg
     4021 gtaaaaatgt attaccatga taataatctt agtgtgtcag aaaagttatt taatagcggc
     4081 ttatgcttac cttctggaag taatatgaca gatagtgaac aagaatttgt tattgatgag
     4141 tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca taggaattat tggtttaggt
     4201 tccatgggga aaagaagact tagactcatt caagaacact ttaatgatat tttactatgt
     4261 ggtgttgata ctaatgaggc gagacgaaag gaagttgaaa aggaatttgg aataccaact
     4321 tataatagtc taacagaggc aaaaaatttg tttgaaataa attcagtttt tgtttgtaca
     4381 tcccctataa cccatgaatt tattataaaa gaggctttaa atttagattg taatatcttt
     4441 acggaaatca atttattaaa tgaatattat gatgaaataa tcgaagtagc taaaaagaaa
     4501 gatagattac tttatctatc ttctacattt ttgaagagaa aagaaattca atatattaag
     4561 tctaaagtga cgcataataa aaaaatttct tatcaatacc atgtaggaca gtatttaccc
     4621 gactggcatc cttgggaaga ttatcgcaat ttttttgttt caaacaaaaa aactaatggg
     4681 tgtagagaaa tattcgctat tgaattacct tggattatag atacatttgg gaaagttaaa
     4741 tcgtttactg taatgaccca gaaattatcc aatcttgata ttgattattc ggacagtttc
     4801 actcttatta ttgagcatga aactggtata gttggaactt taagtgtaga tgttgtttca
     4861 agagtagcta aaagaaattt aactataatt ggtgaaaaga ttttaataga atgggaaggt
     4921 aacccggatt ctctattttc atggaatgaa gaaacttcta gtatgacaga tattcaattg
     4981 tacgatgagg ttatgcatga taataattat tcacatacta ttattgaaga tgcatattta
     5041 gaggagatta aagaattttt cacgcttttg gaaaagaaaa tggaacccca atatactttt
     5101 gaaaaagata agtatattat taacttaatt aacgacattg aggtaaatta atgatgaaag
     5161 taattttttt tgggttaggg tcaattggaa agagacatct aaaaaatcta ataagctatg
     5221 gaaaaaagaa ttctattaat tttgatataa cagcttatag tagcagtgga aaaaaagagt
     5281 ttgaagaaat aacgtatatt caaaatagag ac
//
LOCUS       DACEZN010000169         5155 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEZN010000169 DACEZN010000000
VERSION     DACEZN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.169"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(51..1751)
                     /locus_tag="IXH60_002472"
     CDS             complement(51..1751)
                     /locus_tag="IXH60_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991359.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2258..3418
                     /locus_tag="IXH60_002473"
     CDS             2258..3418
                     /locus_tag="IXH60_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1991360.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3434..3877
                     /locus_tag="IXH60_002474"
     CDS             3434..3877
                     /locus_tag="IXH60_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991361.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4360..4890)
                     /locus_tag="IXH60_002475"
     CDS             complement(4360..4890)
                     /locus_tag="IXH60_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1991362.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4995..>5155
                     /locus_tag="IXH60_002476"
     CDS             4995..>5155
                     /locus_tag="IXH60_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1991363.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEE"
BASE COUNT         1738 a          898 c          973 g         1546 t
ORIGIN      
        1 gattataccg tttggagagt tttttgatcg gatgtattta tcccttttgc ctaagcacgt
       61 aaaagcttgt atctatgata aataaataaa catagcaagg taaaccagcc aatcaaagta
      121 agagaaagaa tgaaatagaa atagattggc gtttgataat gaagcgtgag ctcgttgatt
      181 cctttttgct gcttcacgat tggggtcccg atatctgtta cttcataatc agtcaatgtt
      241 tttccatttt gctgtagcac agtccgatcg tagacgataa cagggatatg aacagttttg
      301 tccccagtgt ttttccactt gattgtcaaa agaccgcctg gatgtgtttt tataaaagtt
      361 gtttcgtttt ctagtatttc ttcgttatac cggtcgtatt tattgtcttt tgtctcctta
      421 tagattggca gatagtcagg tgtggttttt tggaaaacga acaagctggt agacatatct
      481 ttgctgaaga atgttttcct tgcttcttcc ggcgtatcga atagataagt atgtcgactg
      541 acaaatgttt cattttccca cttttccaga tgctggtata agttctggat cgtctgtgct
      601 gaaaaaataa gaatgccaaa tatacttaaa agacggaacc acttttgttc agaccactgg
      661 ttcagtgaaa gggcaaggta aagtagaagc aatagcgtaa atggcacgaa aaatcgaaat
      721 ggaaactgga tcaaactaat aaaaggcaca tttttttcga tcaatgtcgt ccatggaatc
      781 aagctagtag aaagaaaaaa gaaaacaaga cttgtgtaca acacgatttt ttggaataag
      841 gtgttctttt gttgaaagaa aataaaaagc aaaaaacctg ataatatcaa tataggaaag
      901 gccttagggt aatggagcca ataactgcca tccagatcta tcgccttttg actcatttct
      961 ctgttaacaa agggaggaag aatcttattc ccactgtaaa caatagctaa tcctgcccaa
     1021 atatttgctg tcagaacaaa ataaagcaag accgacttaa tcagttgaag cagtgctttt
     1081 cctttttcag tctggttaaa aaagtaataa agataaaaag ggatatacat caaagctaac
     1141 atgatcgaag acagtaaatg gatctgtgtc ataagtccca ctgagacagc gactttgata
     1201 acagggaact ttttatgaag gacgaaatca gcaataggga tcatacataa aggataaaaa
     1261 caagtccccc agctagtgaa tccttggttg atagaccagt attgaataga atacgtactc
     1321 atgaataaaa cggctactgg tacacttacc caatttcgaa cattgacata tctcagtaaa
     1381 atcagcatag aaaatcctga aataagataa agaatgaaat ttgaaagaat ctgataatga
     1441 aaccagctac ctgcaagcaa cacgatcaat ccttgtaaat aagcgaaaaa tgggccgtaa
     1501 acggcattca cgatcctgcc actttgctgg aatccataaa gggaaggaaa ataatggaaa
     1561 tccccatatt taatttgttg cgcggcatca tagaaccgat tgtaatggaa tggggcatca
     1621 gcacccaaaa ttagtttatg tgtgaataaa tgaggcatga gaaatgccaa agcaatgaaa
     1681 aaaattgcta aaaatgggcc atattttttc aattgtcgaa ctcctttgca aactttagat
     1741 aaattcatca tatatcctat gcgagaggta ggcaagaaat tagaaaattc atgaaaatat
     1801 ttatgaattt tacaagaaaa acataagtga aagttctaag gagagaggat gcgacataag
     1861 ttttgttgca tctccttgtt ttgaaacaga atttttctta atactcgaaa caaagtagct
     1921 ttttcatgac ctctcggtaa acggtgttca atcagctgct tctcggtaat aagctcacta
     1981 gcctagaaaa tatgagaagc tattttctga ctagcgctct tattactcaa agcataacgc
     2041 agttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
     2101 aacccaacaa aaaatatgag tagctatttt tttgggctgt acttattact tgatgcagga
     2161 cagctttttc acaacctcat cggctttcat tttctatttt tttcctgaac ccatataata
     2221 aaagaaagaa caaaaaaact tgcaagggag gacacatgtg aagcagagta agcaaaaaca
     2281 gtctcgtcga cttttgtttc gtttgttttt attaatatgt atcgtcgtag ggttactgat
     2341 cgctttatat ccattttatg tggattcttt aaatagtttt attgatcaaa aacgtatcga
     2401 acaagtacaa aaacggaccg cagctgaaat tgaagtgcag cgaaagaaaa tggaggagaa
     2461 aaaccaacga ttaactgacc aaggattcaa tcctggtact gatcctttca atgaaaagaa
     2521 cagaaatgaa agtacaacat ccagccaatt agaggaatgg ctgatcggaa gtatcaatat
     2581 acctaagatc caattaaata tttcacttta tgatcggctc aatggtagga tccttgaaaa
     2641 tggagcaggc gtcttgcaag gaacctcttt cccgcttggt ggaaattcga cgcattcagt
     2701 gatctctgct cacagcggtt tacctaatcg acggttattt accgaattag atcgtttaga
     2761 aaacggagac acgtttattt tgactgtttt gggggagaaa ttggcttatc aagttaaaaa
     2821 tatacaagtg gtactgccag atgacacgtc tgttctaacg atttcagaag gagaagactt
     2881 agttaccttg cttacatgta cgccgtatat gatcaacacg catcgtttgc tagtgacagg
     2941 acatcggatt ccttataacg aatcggtaaa aaaagaagaa ggaaaaggaa atcaggaaag
     3001 aaccatgcgg caatggttta ttcttgcagg aacgataata gctgtagtca tactgttagt
     3061 gtttatcgga cgactaatct atcagtatag attaagtaaa aaagtgcttg atttttcatt
     3121 tattattagt gattcgtcag ggaaaccggt aaatggtagt tcattcattt tgaaacataa
     3181 gaaaaaaaca cttactagaa atggtgtgcc gttttcagta caaagcgacc atcatgggaa
     3241 agtcaagctg gatcaattgc ctggtggaac ttatcgaatc gtttcagctg atgatcctaa
     3301 agttgctgtt tcttttggta ttagaaaagt aaaacaagag aagatatact tttttgaggg
     3361 gcgaaaactt gtaaaagagc ttcaaaaaaa tggtttttat tttaaaatca atgaataagt
     3421 aaggagagaa aaaatgatcc gcacaacaac aaaattgtct gccaaagaac tggaatctgt
     3481 tctatcgatt tggctgcatg ctaacttgga ttctcatgaa ttcattccat caacttattg
     3541 ggagaatcaa gtagaatctg ttaggaaagc tttaccagat gcagtgattt acttagcaga
     3601 aatagatcaa gaaattattg gttttgctgg ggtgattgat cagtttatcg caggaatctt
     3661 tgtgaaaagg gaataccggc aacaaagaat cggtacgcaa ctaatgaaaa aaataaaaga
     3721 aaccaataaa acactgactt tgacagtcta tcaaaaaaat caatcagcta ttcatttcta
     3781 tctttcacaa ggttttacaa ttcaaacaca gcaaaaagat gaagcagtaa atgaggagga
     3841 acttgtgatg aaatggaatc aaaacgctga tttttgatga acagaataac gaaataatgc
     3901 tcccagccta attttttgct gggagcatta tttcattttg aagtcactaa tttaactgtg
     3961 tgaatatgca gttgttacaa tctttgataa acggtgttca atcagctgac cctcggtatt
     4021 agctcaaaaa acggcaaaat atgaggaact atttttccat ttgtttctct aatactcagg
     4081 tcaaaacgct gatttcacaa cctcttgata aacggtgttc aaacggctgc ttctcggtaa
     4141 taagcccact agcctagaaa atatgaaaag ctattttcta actagcgttc ttattactca
     4201 aagcataacg ctgatttcac aacctcttga taaacggtgt tcaatcagct gaccctcggt
     4261 attagctcaa aaaacggcaa aacatgagga actatttttc cgtttctttc tctaatactc
     4321 aggtcagaac gctgatttca caacctctct ttcacaacct tattttactc gtttataatt
     4381 ttcatcgaag tattttccgc tacggatatc atctggcatt ccatcgtatg gttcttcgct
     4441 aataacatct tcattgtcta tacctgcatc acccatataa gtgatcgtag caaattcagg
     4501 aacaagtaat tttggataag tttcatattt ttcacgagct gcttccattt ctgcgatatg
     4561 ttcgttttgg aaagtaacag tgatttctgg atgttcttca acccatttag ggaaaaagat
     4621 cattccgtga agtttctttt catttggcat aaagtccaat gccacgtctg tccctgttgg
     4681 ctctgtccaa gtgattttga atacgccgtc cgtcaattta acgatatcag cttcttggtc
     4741 ttttacccaa cggcctttga ccattccgcc atggatacgg taatcaacag tatggtcatt
     4801 tttggcatac cattcatatt cccaaccatt gtcatacgta tagataaagt gagttccaat
     4861 aaaatcttcc aattctttaa attctttcat gtttaagcat ctccttataa attaaaacat
     4921 gtaataaact acatgttttc gattactcat ttatgataga atggatttaa agatttgtca
     4981 aggagggaaa actgatggca agaaaaaata cacttcgata tattttgtta gggttattaa
     5041 gtaagaagaa gatgacagga tatgaattga atcaatcgtt caaaaatgaa ataggagaat
     5101 tttggcaagc aaaacatagt cagatttatc ctgaacttgc aaaaatggaa gagca
//
LOCUS       DACEZN010000170         5040 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEZN010000170 DACEZN010000000
VERSION     DACEZN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.170"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            97..576
                     /locus_tag="IXH60_002477"
     CDS             97..576
                     /locus_tag="IXH60_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1991364.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            793..1884
                     /gene="phnW"
                     /locus_tag="IXH60_002478"
     CDS             793..1884
                     /gene="phnW"
                     /locus_tag="IXH60_002478"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1991365.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1877..2005
                     /locus_tag="IXH60_002479"
     CDS             1877..2005
                     /locus_tag="IXH60_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1991366.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            2009..2554
                     /locus_tag="IXH60_002480"
     CDS             2009..2554
                     /locus_tag="IXH60_002480"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1991367.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(3008..4699)
                     /locus_tag="IXH60_002481"
     CDS             complement(3008..4699)
                     /locus_tag="IXH60_002481"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1991368.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         1533 a          980 c          954 g         1573 t
ORIGIN      
        1 ataaaataat tttaggtata aaagacgaat attattgggc aatccttctt ttttttgtta
       61 tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc gcaaagctca aagacatttg
      121 ctgattaaac aagtcattga agaattcccg atccgtacgc aagaagaact attaaacaaa
      181 cttgggcatt atggcgttac cgccacgcag gcaacgattt ctcgagatat ccgtgatcta
      241 aaaatcgtga aggcacctga tgaaaatggc gtttcccgct tcgtattgtt tcgcgaaaaa
      301 ggaactcctg agactaggac tgaggaagag gatcggctgg ttcaaatgat tgaagacatt
      361 gtattgaaag tcgaacgagt ccactttttg acgatcgtca ataccttgcc agacaatgct
      421 catttatttg catctgtttt agatgagatc aaacctccct ctattgtcag tacaattgct
      481 ggattcgata cgatcatggt catctcggaa aatgaggagg acgcaaaacg agtagaagaa
      541 tttcttcacc atccaactga agtatcactt ttttaatcag agcacgtatt cctaatatgg
      601 aatgcgtgtt tttttatttt caatattttc cttattgaat aaacctttac actctttaca
      661 aaaatacatc attttttatt caaatttttc aacaccttga ttgaatcaca ataatcatca
      721 tttatattgg atgttaacaa gagcagcttc ttgctctttg tgtatttcaa aaaagaaatg
      781 aaaggaaatc atatgtataa attattaact tctggaccat tgacgacttc tgaaacagtg
      841 aaaaatgcta tgctgatcga tcattgtact tgggatcaag aatacaaaga aattacacaa
      901 tggataagaa aaaaattatt ggatcttgct caagtatcgg aaggaatata cacaacggtc
      961 ttaatgcaag gtagcagctc cttcggggta gaatcggtat taagttctgt gatcaggcca
     1021 gaagatactg tactgatttg ttcaaacggc gcatatagac aacgaatgat caacatgtgc
     1081 gaatgtctga aactgaacta taccgtttat gaggtatcgg aacatcaaat cctccaagct
     1141 catgtgatta cacagctgat tgaagagaat ccgtctatct ccgcactagc aatggtccat
     1201 agcgagacta cttccggtat tttgaataat ttggaagaga ttgctgctgt cacacaagaa
     1261 agagatcttt tgtttatagt ggacgcgatg tcaagctttg gtgggatacc gatttgtgta
     1321 gccgaactag ggattgattt tttgatttcc agctcaaaca aatgtattca aggtgttcca
     1381 ggcttttctt ttgtacttgc taataaagcg gaactggaaa aacgaaaaaa tgttagccga
     1441 agtttgtcac ttgatctata tgaccagtac cgcgtgatgg aaaacgagaa tggcaaatgg
     1501 cgtttcactt cccctaccca tacggttttg gcttttcaac aagcgctgaa ggaactagag
     1561 gcagaaggag gaatcgaaac tcgatattca cgttatctcg ccaataatca tgtgctacga
     1621 caaaaaatga cagagatggg gttccagcct ttcattcaag aatatcaggg gccattcatc
     1681 acctcttttc tttaccctaa ggactggcga tttgatttta atcattttta tcagtattta
     1741 aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg atcatgaatg ttttcgtatc
     1801 ggaaatattg gcgagatcta tccggaagat attttggaac tgacagcaat tatcaaagac
     1861 tataaggaga acgttaatga ttaaaactgt aatttttgat tgggcaggaa caactgtgga
     1921 ctttggctgt atggctcctg tccacgcttt tagaaatgct tttctagaaa aaggaaccca
     1981 actgactgac aaagaaatcc gctaaccgat gggaaaatta aaatgggatc atatccaaca
     2041 gcttctttcc ctcccttctg tcaaaaaaca atggatagct atttatggac aactacctca
     2101 ggaaaaagag gtggaggaat tgtatcaaca atttgaacat tatctgttta aagatttggt
     2161 caagcattct accttgaaac ccgacacatt ggaaacgatc gaacggttga gaaacgctgg
     2221 gatcaatatt ggttccacga atggatttaa tgctcctatg atgacaatcg tagcagaaac
     2281 cgcaaaaaaa gaaaggtatt caccagactt catggcaact tttgaagacg tcgaagggta
     2341 tggtcgaccg tttccttata tgatccataa aaacatgcag tacttcaaaa acaagtcggt
     2401 tgatgaagta atcaaagtcg gagatacggt ctcagatatc caggaaggga aaaatgccgg
     2461 agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg gggatttctt atgatgagta
     2521 ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt ctgttgctct tagattccaa
     2581 caagccggcg ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac
     2641 gctgacaaga atccagaatg acttttatac aaaataagag gttgtgaaaa agctgtcctg
     2701 tatcaagtaa taaaaacagc caagcaaaaa tagctcctca tatttttcgt ttggttaggc
     2761 ttattaccgc agatgcaagc ttttgaacac cgtttattcg aaaaaaaaaa gggagctgtg
     2821 acacgacttt tgtcacagtc cccttatttg caaacaaaca gtgtttaaga gacaacctct
     2881 ccactaaacg gtgttcattc agctgactct cggtatttgc tcaaaaaacg ataaaatttg
     2941 aaaagctatt ttctcttttg tttctctaat actcggatca gaacgctgat ttcacattct
     3001 ctttttctta catcttatct ggtgcatgaa gtcctagcaa acgaaggtct tctttcaata
     3061 atacagtcac tgcataaact agagctaagc gtgcttcttt ttgttcatca tcagctaaaa
     3121 tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa tttgattgca tgtttcgcaa
     3181 tcactgacgg ttcgtatttt tccccagcag acaagaccgt ttcagggtat ttttgaacta
     3241 atttaaccac ttcccagctg gtatcgtcat tcaatgcata gtctgctgtt tctgaaggga
     3301 caaatcctgc tttttccaac aggctgactg cacgagcatg tgtatattgc acataaggtc
     3361 cagtttctcc ttcaaatcga acaacttctt ccaggttaaa gtcaaatgta ttcagtcgat
     3421 cgtttttcaa atcgtggaaa atgactgccc caaccccaac ttgtttagct acagcatctt
     3481 tgttttccaa atcagggttt ttctcgctga tttgttcttt agcagattgg attgcttcat
     3541 ttaatacttc ttctaataag acgatctttc ctttacgagt tgataatttt ttaccacctt
     3601 gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga ccagtcaaag cccatttcct
     3661 tcaagacagc tttcaactgt ttaaaatgat agctttgttc attccctact acatatagag
     3721 attgtttgaa gtcgtaagta cgtttacggt acaatgcagc agctaagtca cgtgtaatgt
     3781 atagtgtcgc accgtctgac ttcttaatca acgctggatt caggtcataa gcagataggt
     3841 caacgatctc tgcaccttta tcctcgttca gcaaatgttt ttcttctaaa agtttgacga
     3901 tttcatccat cttgtcattg tagaaagctt caccgttcaa cgaatcgaaa cgtacttcca
     3961 gcagatcata gattttattg aattctttca tcgattcatc acggaaccat tgccataatt
     4021 gaatagcctc tttatcgcct tcttccagac gtttgaacca agcacgagct tcttcattta
     4081 attctggttc tgtttcagct acttcatgga attggacgta taaacgaagc aattcattga
     4141 ttggttctgc tttaacagct tcttctgtac cccatttttt gtaagcgacg atcagttttc
     4201 caaactgtgt tccccagtca cctaaatggt tgatgcggat tggctgataa ccgatttttt
     4261 ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg caaatgtccc attgagattg
     4321 gtttggcaat attaggagaa gacatgtcga ttggtactgt tccttgattc ccgatgttgc
     4381 tatccccata atgttctttt tctttcacta ctgtttgcaa aacttttttg ctgataagtt
     4441 ctttattcat gaaaaagttc aagtaaggtc cgacaacttc gattttttca aagttggcag
     4501 aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg cgcttttcgg taaacttttg
     4561 ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc tgattttgga ttttctagta
     4621 attgctcgac ttgttccagt gttaaatcat ctttgacagc gtcatagaca gcttttgcga
     4681 caagatcttt attattcatc tttattcctc ctaaagtgtt cccttgattg ttaaacaaaa
     4741 aaaatctcta gcgacggcat acttagtatg ccatcactag agacgagatt tcccgcggta
     4801 ccactccaat tgtcctaaaa aaggaccagc tccatctgat atcggtagac gaccgtctgt
     4861 ttaaaacaga attctccaag tgcgcttcgt tgtatcttct tcattcggct tccaccactt
     4921 ccgaattcgc tagccaagaa tgatacaagt actctcttgt tcaacaaatc ttagtcttat
     4981 taaactttct tgaattatat cagaaaaaaa aacgaaaggc tagtcgtttc ataaaaaaaa
//
LOCUS       DACEZN010000171         5010 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEZN010000171 DACEZN010000000
VERSION     DACEZN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.171"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..295)
                     /locus_tag="IXH60_002482"
     CDS             complement(<1..295)
                     /locus_tag="IXH60_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991369.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQL"
     gene            complement(319..1290)
                     /locus_tag="IXH60_002483"
     CDS             complement(319..1290)
                     /locus_tag="IXH60_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1991370.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1996..3375
                     /gene="brnQ"
                     /locus_tag="IXH60_002484"
     CDS             1996..3375
                     /gene="brnQ"
                     /locus_tag="IXH60_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1991371.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(3531..3977)
                     /locus_tag="IXH60_002485"
     CDS             complement(3531..3977)
                     /locus_tag="IXH60_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1991372.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(4004..4180)
                     /locus_tag="IXH60_002486"
     CDS             complement(4004..4180)
                     /locus_tag="IXH60_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1991373.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(4342..4770)
                     /locus_tag="IXH60_002487"
     CDS             complement(4342..4770)
                     /locus_tag="IXH60_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1991374.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            4862..>5010
                     /locus_tag="IXH60_002488"
     CDS             4862..>5010
                     /locus_tag="IXH60_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1991375.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDE"
BASE COUNT         1377 a         1033 c          889 g         1711 t
ORIGIN      
        1 ataattgctt gatccgattg ttctggtcat ctgccagatc tttttgataa gtatattctg
       61 gagtcactgc ttctgctgct agttttcttc tttgttcggt ttcttccgtg ttctcgatcg
      121 ttttattcgc acggatactc tcttctgcta cttggccttc tctaaaatca ttggcctttt
      181 gtttgacact actaaatgta acgccgcaaa caatgataaa aaacaagaac agaaggaaag
      241 gcaatgctgc ctttccgagt ttttcttgta gtttctgcag tgttttattc aacatgtgct
      301 tatcctccta agcaaagctt attcttgatg aagatccgcc ttttcataag ctttgatgat
      361 ttcggcaact acagggtgcc gaacaacgtc acttgcttca aaattaacaa aatcgatttt
      421 tttgatttgc tttagtgttc gttctgcatg aacaagacca cttgtcgtcc ctcgaggcaa
      481 gtctatctga cttgtatccc cattgacaat catttttgat tgataaccga gacgggtcaa
      541 gaacattttc atctgagcga ctgtcgtgtt ttgtgcttcg tccaagataa cgaatgcatc
      601 atccaatgtc cgcccccgca tgtaagctaa tggtgcgatt tcgattacgc ctcgttccat
      661 caatcggttc gtatgatcca atccaaaaat ttggtataaa gcatcataaa caggccgcag
      721 ataaggatcc actttttctt tcagatcccc tggtagaaag cctaggtttt cccctgcctc
      781 tacagcgggc cgcgtcaaaa tgattttttg cacttcgcct ttttttaagg cagcaattgc
      841 taatactacg gcaagaaacg ttttccctgt tccggctggc cctacaccaa atacgatgtc
      901 atgtttggct accgattcaa catatttctt ttgaccgctg ttttttaccc gtataggttt
      961 tccattacgg tctttgacga tctcttcctc atacatctca acaaaataat ctaatgtacc
     1021 tttttgcccc attttcaatg ccgtcacaac atcgggcgta cttacgtgga ttcctcgttt
     1081 gatcaattcc tgtaatgtcc gaatgactga ggcagccaag gaaatctggg attgttcacc
     1141 aatgatctga atgatttctc cacgtgtatg gatggttgtt tccgttgttt cctcgatcag
     1201 ttttatatgc ttgtcgtgtg cacctaaaag catgctgaca tcatcagcag ctgtcaatct
     1261 aatctctaaa gaactagact cttccgtcaa aaatagacat cccctttttt tcgattcatt
     1321 ctttcctatt ttaccatact cagaagaaac tatatagcaa ataagaaaag gttgtaaaag
     1381 agtcgtttaa ccatacgtac caagaaacgt taaaaaatag ccgtttccat tcgctaaagg
     1441 tctcgttttg ataaaaagaa tctgagaatg taagttatat gaaagtggag aacaaaatat
     1501 tccgtttcat tcagcaggta ttctctaggt atttttcttg tttcaaaaaa agaaaacaaa
     1561 atagttaaaa tattcttgca tttcaaaaaa aacaggtgta ttattacgtc aacaaataaa
     1621 catacagcaa agcgtcgagg aaaacagtag gctattcaac gatttattcc cagtgagtca
     1681 tgtaagtgag aaatgacaat aaatcgaata gacgaacatc atttccgagt caatgtcgaa
     1741 aacgtagatc attgtggctg cctccattat caggctagcg gataacttta tcaaaaagcg
     1801 tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt accgttctag aatctagagc
     1861 ccttttccca agtggaaaag ggcttttttt atattttaag gtggtggttt tcatggaatc
     1921 tgatattaat ggcattacta attgctgcaa cgtttatatc gtcaatcaaa atgataatta
     1981 ttggaggaaa aatttatgga agaaacaaaa acttattcat ggaaacaact attgatggtc
     2041 agttcactta tcttcgggat gttcttcggg tcaggtaatc taatctttcc tgtccactta
     2101 ggtcaaatgg ctggcacaaa ctgggtcagt gctggcgtag gatttgccat ctcaggcgct
     2161 cttttcccat tactagctat cttagctgta gtagtgacaa aaagtgatgg tctgtatgac
     2221 ttagctaaac ctgtcggaaa atggtatgct gccttgttct taatccttgt tcatttgacg
     2281 atcggaccat ttttcggtac accacggaca gctgctactg cttttgaaat ggctgctaaa
     2341 ccatttttgc ctgaaaaata taattcatta ggtatgtttt tattctctgc tcttttcttt
     2401 ttacttgctt atttccttac tgtccatccc acacggttgg tcaaatacgt cggaaagtat
     2461 ctaaactcct tgtttcttgc attattagca atcatttttg tcgtttcttt attccaccca
     2521 atgggaaatc taaatcaatc tgctgcagat acttaccaaa caaacgcttt aacaaatggt
     2581 gtcctagaag gttataacac ggttgatgca gtagctttgc ttgccttaag tgtcactttt
     2641 gtccatgcag tccgtgacct tggtttcaaa gataaaaaag tcacagaatt aacagcaaaa
     2701 gctggtagcc tgagcgttgc gctagaagtc atgatctatt ttggacttgt tcttctcggt
     2761 gcgatgagtt tgaataagat gtctttatca gaaaatggcg gaactgcact ttctcagatt
     2821 actaattttt atttaggtaa cttcggcgct agcttcttag gagtgatggt cacattaggt
     2881 gtcttcacta ccgctatggg actcgttgtt tcttttgcac aagatttcca taaactgttc
     2941 cctaaagtca gttatatgac atggctgcgg ttaacgactt tcgtctcttt tgtcgttgca
     3001 aatgctggtt tggacaatat catccaatgg tctttaccag tattgatgct tttgtaccca
     3061 ttgtcgcttg cgttgatctt gctttcctta acagcaaaat ttttccaaaa gacaccattt
     3121 gtctatcaag taacgatgtt gtttgctgct gttcccgccg tattggatat gctggcaaat
     3181 tcgcctgctt tagtgagtca acaaagagtt gtagcaagca tgctcgaatt ttatcatcat
     3241 cacgttcctt ttgctgcatt aggacttggc tggatggtcc caacgttact tggttacgct
     3301 ggcagcttac tcttctatta cgcctatcgc ctatcagggt acaaacaaga agcaaatgaa
     3361 ctaccagaag aataaccaga tcaaacacaa tttaacataa gaaatattaa acaggaaaaa
     3421 accagctaga tgaaagaatc ctattctttc gtctagctat tttttagatt tcttttttac
     3481 gagcacaaaa aaactgcgga caatgattgt ccgcagttta aatgataaaa ttaagattgt
     3541 aatagttctt tcacaatggc atttacttgg tttccatctg cttttccttt gactttaggc
     3601 atgatcgcac ccatgacttt gccaaattct ttcattgatg aagcaccagt ttgtgtgatt
     3661 gcttcttgaa caagttgtcg aatttcttcg tcggaaagtt gttccggcat gtatttttcg
     3721 actatcacaa tttcactttt tactttttca gctaaatcgt cacgtcctgc ttcttcaaac
     3781 tcatgcaaag aatcacgtcg ctgtttcatt tcgcgagaca acaccgttaa ttcttcttct
     3841 tctgtcaagt cgtgaccttc tttaatctgc tcgttttgta tagatgactt gatcattcgg
     3901 atgacttgta aagattcctt atctttcgct ttcatcgctg ttttcatgtc atcgtttaat
     3961 ctacttagta gtgacacact accaactcca attcatcaca gaactagaat tttttgcgtt
     4021 ttctagctgc ttcagatttt ttcttgcgtc ttacacttgg tttttcgtag aattcacgtt
     4081 tacgagattc ttgtaaagta cccgcttttg acacggaacg tttgaagcga cgaagagcat
     4141 cgtcaagtga ttcgtttttg cgaacgactg tttttgacat gttaattccc tccctccgag
     4201 cttaaaaagt aaccgtaatt gttattgaaa tatatctcaa aaaacaagac tgtcctgtaa
     4261 acattatatc taatgctgtt ttgaccgtca aggaaatctt gctttttttt aaaattatca
     4321 aatgttattt tatttaaatc cttattgaca attttcacat cttccgaaaa tctcgaatct
     4381 atgcccttcg atcgtacatc caccgagctg ttcttcgaaa taattcattg gacacatatg
     4441 gatttctttc gttgttccgc acaccgtaca gataaagtga tggtgatggc cgatttcccc
     4501 ttgacagcaa tgaaaacgaa acttcatctc tccatttaaa tcggtttctt ccagtaaatc
     4561 gatttctgca aaatcatgga gatttcgata aatcgtatcg taacttactc ctttgaattc
     4621 ctgattcata tagtcaaaaa cttctttagc ggatacgtag cgattgcgct tcactaaata
     4681 agttaacaat gcttctcttt ttttagtata tttaaaaccg ttatttttca atgtttctag
     4741 tgattgttcg atcaatgtct gttttttcat gacttattcc tccaaatcaa aatgattacg
     4801 aagtacacat tctagagtat ggtataataa acataaaaaa aatgcaagtg aggttttatc
     4861 catgtcttcc gattctacag aaatcgttac tttttttgtc atctccgact ctgctggaga
     4921 aactgcgaca aaattagcgc aagccactat ggcccaatat cctagcgttg agttcaattt
     4981 attccgcaga acatttgtga cagatgagaa
//
LOCUS       DACEZN010000172         4988 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEZN010000172 DACEZN010000000
VERSION     DACEZN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.172"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..545
                     /locus_tag="IXH60_002489"
     CDS             <1..545
                     /locus_tag="IXH60_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991376.1"
                     /translation="FIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLLYGKIVEIL
                     EEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFH
                     LYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTKIYPISLKG
                     ANIHLYHIAQELHLLKKHIS"
     gene            766..2529
                     /locus_tag="IXH60_002490"
     CDS             766..2529
                     /locus_tag="IXH60_002490"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1991377.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2613..3071
                     /locus_tag="IXH60_002491"
     CDS             2613..3071
                     /locus_tag="IXH60_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1991378.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3180..3863
                     /locus_tag="IXH60_002492"
     CDS             3180..3863
                     /locus_tag="IXH60_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1991379.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4010..4624
                     /locus_tag="IXH60_002493"
     CDS             4010..4624
                     /locus_tag="IXH60_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1991380.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4640..4846
                     /locus_tag="IXH60_002494"
     CDS             4640..4846
                     /locus_tag="IXH60_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991381.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1778 a          795 c         1015 g         1400 t
ORIGIN      
        1 attttatttt caagcaatcc tttttacaat ttgtccgtac gttgtgggca gatggacaag
       61 tatttttacc ggaaaggatc tatcttctat cagaaaaaga acaacttctg tacggaaaaa
      121 ttgtagaaat attggaagag tggaaaaaga aaaatcaaat ccaactaaga tggaatgaaa
      181 atttcattcg aaaattcgtc aaaagtcttt ctttgatcaa cagtgaacaa gcggggagac
      241 aggaaataga aatttttgtt gtttcagaaa gttcagtcaa acaatttttt tatcgaaccc
      301 aatttcattt gtatgcggaa gaaggcatcg ctgaaataaa tcctatttta tttcgttcat
      361 tagaagaatt gcctgatgaa tgtctatgtg cgaaaaaaag agtggttttg tgcgatgctg
      421 ctagctatca aaatggatta gaaactgaaa agacaaagat ctatccaatc tcattaaaag
      481 gtgcaaacat ccatttgtat catattgctc aggaacttca cctattaaaa aaacatattt
      541 cttgatgaga ccttaattag gagaccttct acaaagcttt ttaattgtct gtagaaggtt
      601 tttatttgag tcctgattcg tgcgaagatc tacttatata acaagctttc tctgaaattt
      661 ttttaaaatg ctattgactt agagtcaact ctaagtagta gaatagataa agtgtaacaa
      721 atctattttc attaaaagaa gatagtacgg aggagagaat gtattatgag tacagtatta
      781 catgtattgg atgcaatcaa tagccccgca gatgtcaaaa aattatctgt tgaggaaatg
      841 aaacagttgg cagaagagat gcgccaaaaa atcttgcaaa aggacagcga tgtaggtggg
      901 cacgttggtc cgaatctagg tgtagtagaa ttaacgattg cgtttcatta tgtatttgat
      961 tcaccaaagg ataaaatcgt ttgggatgtt tcccatcagt cttatggtca taagctttta
     1021 actggtcgaa aaaatggttt tgaaaaagga cattaccgtg aagtgagcgg atattccaac
     1081 caacatgaaa gtaaacatga ctattttaca gtggggcaca catctacatc tatcagctta
     1141 gcaacaggtt tagctaaagc gagagatctg aagaaagaat caggaaatgt ggtagcattt
     1201 atcggtgacg gctccctaag tggtggctta gcttttgaag gattgaacaa tggtggtgtt
     1261 ttacaatcca atctaatcgt attagtaaac gataatgaga tgtctattga tgagaatcat
     1321 ggcagtttat ataaaaactt agctgagctt cgtcaaacca acggtaaaag cagcaataac
     1381 ctatttaaag caatgggatt tgattatcgt tacgtggata atggaaacga tctggaaacg
     1441 gtgatccgtt tatttgaaga agtaaaagat attgatcatc cgattgtact tcacgtccat
     1501 acggaaaaag gtcgcggtta tcagccggca atcgataata aaatgaaata tcattggcat
     1561 gtaccatttg atctgtccac cggggaggca aagcaagtaa cttctggaga aacatttaat
     1621 atgatcatca tggattattt ggataagaaa atgcaaacaa gtacagctcc tattgccgca
     1681 gtcaatgcag cgataccagg cgcctttgga ctgaaaacat tccaagaaaa acatccagat
     1741 cattattttg acgtggggat cgctgaacag cactctatca ctttcgcttc tgctttggct
     1801 tctcagggag tacgtccagt cgtcttccaa tcaagtactt tcttgcaacg ggcatatgat
     1861 caattatcac atgatttagc gatcaacgag aatccagtga ttattttagt cggtggcgga
     1921 acgatctcag ctagtgaccg aacacaccaa ggaacatttg acattcctta cttatcaagt
     1981 atcccgaaca tcaaatatct tgctccagca acaaaagagg atatgatctc tatgctagat
     2041 tgggcgttcg aacaaacagc gcagcctgta gcaatccgaa ttcctacaca tggtgtagaa
     2101 catggtacag ctatcgcaaa tgattatagt attccagcct atcaaacagt aaataatgga
     2161 ggaaaagtag caattttagc tttaggcggt tttcttgaat taggaaaaga aacagtaaaa
     2221 gcttttagaa aaaaaggaat cgaagcaacg ttgatcaatc caggaacgat cacagaaaat
     2281 gacgtagaaa cattagataa attaaaagaa acacacgaat tagtcatcac attagaagat
     2341 ggtagtttgt caggaggatt tggcgaaaat atcagtcgtt attatggtga ttcttcaatg
     2401 aacgtattaa attttggtgc agagaaagaa ttcacggata gcgttcctgt agggcagctt
     2461 tatgaacgtt atcatttaac accaaaacaa atcgttgaag atagtctgcg tgtcttgaat
     2521 attcaataat tcattatgct caccatccag ataattgttt atactagtat ctggatggtt
     2581 ttttcattat aaaaagcaaa ggggtggatg ttatgtatca gcaagtagaa gaatatgtca
     2641 aaaagttcca agaaaaatat cctagttatc aatcagaaaa attaggtttc ttttcatttg
     2701 gggatacaga aaaaatggca gacgagttga gcgaactagt tgtaaaaaag ataaagactg
     2761 gtacatcaag tggttacgat ctgtataaac aagagaatga gcctttacca aaagttggtc
     2821 aattagacgt ggtcctgaat ggaaaaaatg aaccagtttg tatcatcaaa aatataaatg
     2881 tgaccatctt gccttttagt gaagtcacga aagagcatgc ttggaaagaa ggagaagggg
     2941 atcggacatt agcttattgg cggaaagtcc atgctgagtt ttttaaacat gcttataaag
     3001 aagcgttgaa catagaattt acagaagaga agctggtcgt atacgaagaa tttgaagtaa
     3061 ttttctttta gtaagaaagg tctgctgaaa gaaatagctc attattgtct cctgaataga
     3121 aaagggttac atttttatat gaagctgtat ttttatataa cattactgga ggagatattg
     3181 tggatacact ggaacaaatc aaaggcggag tcgtcgtttc ttgtcaagct ttggaaaacg
     3241 agccgctaca cagttctttt attatgggga gaatggcttt ggctgcaaaa gaaggcggag
     3301 cagttggtat tcgagcaaat acgacagaag atatcaatga aatcaagcga atcgttgatt
     3361 taccggtaat agggattatc aaaagagatt acgatgggtc agaagtgttt attactgcga
     3421 cagaaaaaga agtggaagaa cttttggaga cgcaagcaga aatcattgca ctggatgcaa
     3481 cttctcgtaa acgtccgaca gaaaaaacaa ctgctgaact tgtccaaatg attcacgaac
     3541 acggtcggtt agctatggca gatatttcga cttttgagga agcgcttcaa gcgcaagaag
     3601 atggttttga tcttttgtct acgactttag caggctatac agaatattca cgaaagacgc
     3661 agacacctga ttttgattta ttgaatcaaa tcatagaaca agtcgatatc ccagtaatta
     3721 tggaaggaca tactgactct cctgaacaag tagagaaagc atatgaatta ggtgcttttt
     3781 cagtcgtagt tggatctatt atcacccgtc cgcaattgat cactaaaaga tatgtcgaag
     3841 ccgcaagtaa agcaaataac taaaaaacac gcacggcaca gattcttgac aaaaaagaaa
     3901 aattgataga tagtttaaag cagattctaa gaagatgaaa aaagcattac ttttcatttt
     3961 gtcttcaaga tctgcttttt aacgaatttt atagaaagaa ggcgaaacca tgtttgaacg
     4021 gagtccttca gaattgacag aacgtgaaaa ttacaagttg ctgattggtt cagtcatccc
     4081 tagaccagta gcagtagtaa cgactaaatc agcaaaagat atagtgaata tcgcaccctt
     4141 cagttacttc aatatcgtca gttcgaatcg acccatctta tccttggcga tccagagaaa
     4201 acaagatgca gtaaaagaca cagcaaagaa tattcttgag acaagagaag ctgttatcca
     4261 tatccttgat gaggacaacg tagaagaagt aaataaaaca gcagccaatc tgcctaatga
     4321 ggaaagcgaa ctgtacctga ctgacttgac tttaactgat tcagtaatca tttcagtacc
     4381 agcattgaca gaagcaaaag ttcgttttga aacatcctta tatgaacatg tcgagatacg
     4441 gaataaaaat aaagtaacag ctgatttatt tttgctagaa gtacagaaat atcatttaaa
     4501 tgaaacagtc tatgatgata agacaggaag gatcgatcca agaaaattga atgctgtaag
     4561 ccgtttggca ggaaatgatt atgcagcaat aggagaaatt ttcacgattg cacgtcccga
     4621 ataaaaagga gcactagtta tgttaaaaat aagctataaa ccatcgacag attcaaaaga
     4681 aatgaaaaaa gaatatgaaa cagtgaatga ttttctgcaa gggcagtact tagaagttcc
     4741 gccattgcaa gaccattttg tcgttactac tgttacttta gatggtaagg aaatagaaat
     4801 gccagatcaa acgattagcg gattgtttaa ttactttaac aaataaatat ttattccgaa
     4861 aagtgaaaaa acatataaaa aaacacataa aactaaaaaa ttatcaaaga atcctggtgc
     4921 aactgaaaga atttgcaacg ggattctttc aacttatttg ttattttttt ctaaaaaata
     4981 ttaaagat
//
LOCUS       DACEZN010000173         4915 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEZN010000173 DACEZN010000000
VERSION     DACEZN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.173"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            1596..1910
                     /locus_tag="IXH60_002495"
     CDS             1596..1910
                     /locus_tag="IXH60_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991382.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(1963..2736)
                     /locus_tag="IXH60_002496"
     CDS             complement(1963..2736)
                     /locus_tag="IXH60_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991383.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLNVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            2841..3086
                     /locus_tag="IXH60_002497"
     CDS             2841..3086
                     /locus_tag="IXH60_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1991384.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            3242..4093
                     /locus_tag="IXH60_002498"
     CDS             3242..4093
                     /locus_tag="IXH60_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991385.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            4126..>4915
                     /locus_tag="IXH60_002499"
     CDS             4126..>4915
                     /locus_tag="IXH60_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991386.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINV"
BASE COUNT         1904 a          640 c          830 g         1541 t
ORIGIN      
        1 atgagataaa aaacaacttg aaatattgtt atctagaaaa aagtgaagag ataatccatg
       61 aattaaatgg cttttaaaaa ttgtatttga catgaattta cgacaaggaa ttagtgggga
      121 ttaagcgatt attctcttcc actttagaga aataatgggt taaaaagcca gaatacgtag
      181 agcaatattg acaacctata aaggagctat tgttgaaaat tgaagtaaca aaaagataaa
      241 gagaggttta catctatcag attaaaagtg atagtacacc catgaaacct gcaaaagagt
      301 tgaacctaat gtatatcggt ttaactcttt ttatttgcaa attaaaaaaa tatcaccgaa
      361 gtgacgaatg aaactatgac caaattaagc aagaaaatat atcttgcaaa taaagtagta
      421 taaaaagata ttgataaagc caatcaaaac tttgaatttc aaacgttaga taatcaaaca
      481 gatacaaaaa atgaaatcag ttctacgcgt acgattatta aaaataataa attttcacta
      541 gaagaaaatg gaaataaaat caaataatta ggaagaaaaa acaaagcaat tagaaaagcc
      601 tgcaagcaaa tttgagtaat cgagatccaa tttttttaag gaagtttaac aaaaatatgt
      661 aattctatta tcactattaa agagaaagtt ggagcggaat ataaagaaaa gagaaaagta
      721 attaaacgca aaaaaatgta gaacttaaaa aatataaaga ggaacaaccg ttaaaattag
      781 gaatgatgtt gtgtaaacat tataattgct atgatataaa tgaagtgtta aagaaagcaa
      841 atttaaacca attttgtgaa cagaaaagcc ttagtcacta tgaccaaaac tacggacgaa
      901 actatgacca aaagtaggta ctaaattatg accgaaaata ggtccaaaat ggattttttt
      961 atgatatcaa aaattatgaa atgattttga aagaaaacca gttggcacag ccaacttagc
     1021 caccttcctg taggaaggtg ctatcagttt tcccttatgc tcttttcttt cttttttgtt
     1081 tcatatgtca aatgccattt tgaaatgtac catcttaaga aatttatcct taaaaacaat
     1141 tgcgttattg gataaaaaga tgctacatta tcttaaaaaa ggaatcaaat atgatgaatt
     1201 tagtggctag catatcgtgt gattaagata ctttatagtg taagttgaag gagaaaaaat
     1261 atttaaaata tacttcccat ttcagtggga agtaaaatta atcttgttgt ttctactagt
     1321 aatgtatctt tttggaaact atgtaacgat gtaaacaaga tttttttcag aagaaaaaac
     1381 gataaaagtt gtgataaacg atttaaaaaa taggagaaaa tatatcaagc gcatggtatg
     1441 aaacgcacat ttaaaaagat tcgactaact aaaaaaataa gtttacaaag cattgttgtg
     1501 tattacaagt gatctataca ctgaaaaatg atagagaaaa tctgatacgt ccgcaacaat
     1561 ggacaattga aaatgaaagt gaggaaattg gaagaatggc tgatgaaaat aaagttgttc
     1621 cgcttgaaac actagtgata aataaggaag agtttgagca actttttctt gatttttttg
     1681 ttcaatatga caggatttat gattttagtt cgtcactaga tttatttgaa ttagaggcag
     1741 aaataataga aaacagtcga gaggagaatg gtaaaatcat catttcagta gaaaaattca
     1801 atgaattatt ctcagaaaca tttgaaatag atacagatta ttatcaacca caagaagttt
     1861 tccaatcatt tagaaatcag attattcgct caggtaagac cgtaaactga aaaacaataa
     1921 aagaaacgtt gaaagaataa ttattccttc aacgtttctt ttttagaatg ttttaatgac
     1981 caaagcagtt tcattgtcag gcttaggtaa ttcatgaaat cccgtattta tagtgctcaa
     2041 ttgatcaact aaccatgttt tactttttga attgtagata aatctaaaat taatatcgtt
     2101 attatcgtgt tgaaggggcg tctccaaata atctgctgta tattcatagt caacgtaacc
     2161 tgctaataca aaagagagta tccctttatt taatgttaga tttaatggat aatcatacga
     2221 ctttattttt ttcaacttca tattgtacat atcttcagcc ataaaagttg agttcgtgat
     2281 ttcatcttgg gtctcattaa aaaagttatc cgaataatcg cttatttttg aaaagtcttt
     2341 attggcacca gcctcaaaaa attgttcaac tttttcagta taaagttgtg ctaacgctcc
     2401 tttttcaata ttaactttaa tattttcatg tctatcaatt tgtgtgctgt tcagtaaggg
     2461 aagatcagct tttagtttta tatcgtcttg atttctttca atttcaattg gcaaaatctt
     2521 ttcagtagat tcagtttctg ataaaggaac atttagtttt ttatcatgat aataagcttc
     2581 aaatccatct ggaaccggaa aaaaatgtat tgggtaattc ttttctgagg ttgttgtact
     2641 agtttccgta cttgtagaac ttgtgtcaga agtggtacac ccgcttatag ttccgatgag
     2701 taaagcgcaa gttagaatga ttgcaaattt cttcaaaaaa attcctcctt aaaattgaat
     2761 atggactaat actagtccca ttgtagcata ccgaatcttt aagattaata agaaaaagga
     2821 ctaattttag tccaaaaggt gtgctattct tgaaagagga aaaaattatt gataaagaaa
     2881 atgtaatcgg tagcaatatt agacgagtcc gattagaaaa aggaattggt caaaccgagc
     2941 tgattagaga cctacagctt cgtaaaattg ctataactag ggaaacgttg gttaagattg
     3001 aaggtggccg ccaacacatt aagttggatc aattgaaagc catcaaagat attctacagg
     3061 tgtcttatga agatttgctt caatgactac ttgtagtcat ttcgtttgaa attattatgt
     3121 tacaccctta tgtgtcacga agaaatactg gtataataat gaatgggata tgtaacgata
     3181 gggtatactg ttatgttgcc taataaattg tggatcaaaa tacaagaatg gagggatatc
     3241 cttgtataag gcatatgaat tggattttac aaatgctgac ttaaatgcat tctcctcaag
     3301 ctcgcaatca tatacacatg tagtaaatca aatagatcac aaccaaaaat ctattaacaa
     3361 gagaatagaa aatctatttg aaagtgaaaa tgatttagtt gattattatt ctaaggacag
     3421 caaaattcta gatgcagacg aaattattaa agattggttt ccaactataa aagcagatat
     3481 ttttatatct cactctcatg ctgatgaaaa attagctata cgttttgcat catggctatt
     3541 tgaaaatttt ggtcttactg catttattga ttcttcggtt tggggataca gttctgattt
     3601 attaaaaaaa atagaccaaa aatattgcta taaggaacaa acaaaaacat atgattatga
     3661 taaaagaaat gtaacaacta gtcatgttca tatgatgctt tctacagctt taaataatat
     3721 gattgatagt acagaatgtc tgttttttct taatacacct aattcaatta gtttgagcaa
     3781 tgaaatcact aatgaacaga aatttacgta ttcgccatgg ctatatagcg aactgacgac
     3841 agcttcaata gtggaaaaaa agaatccacg attggaaagt aatcctcaaa tgagcaccga
     3901 ggatgttaga agcattataa agcactattc ttcgcaagaa aatctgcaaa taaaatattc
     3961 tttaaattta agtgaattaa cttatttacg aaaagctgat ttgaaaaaat gggctttaga
     4021 tgctgtggaa tgcactaaag gaagtaatac attagatatt ttatataaaa acaccatttt
     4081 cactaatctc taatcaattt actgaatcga aaggaaccgg gtagaatgga taagaaagtt
     4141 ttaataaaga ggttctctga agctattgaa aaaggaaatg cagcaatatt tgcaggtgca
     4201 ggattatcga tgtcacaagg atatgtaagt tggccggaac tacttaatga tcctgcaaca
     4261 gaaatcgggt tggattctaa aaaagaaaca gacttagtta ctctggctca atattataaa
     4321 aatgaaaatg gtggatcaag aggaattttg aatcagatac ttatggataa ttttggtgaa
     4381 gaattagaaa tatctgaaaa tcatcgaata ctagcaagtt tacctattga aacttattgg
     4441 acaactaatt atgatcatct aatagaaaaa tcaatccgag aagcttataa aaaccctcag
     4501 gttaagaaaa attatactca gttagcaaca actaatccaa atgtggatac aattgtttat
     4561 aaaatgcatg gtgatataga tgatgtcagt tcgacagtaa taacaagaga tgattatgaa
     4621 aaatatgatg atgattcgta cgcgttattt aaagaaactt taaaaggaga tttgttaaca
     4681 aaaacttttc tctttttagg atttagtttt acagacccta atttagaaag aattttatca
     4741 gatataagat gggttttgag agaaaaccaa agacctcatt attgtataat gagaaaaata
     4801 ttaaaggaaa attttgtcga ttctgaggat ttttttgatc aagagagata taattatgaa
     4861 ctgacaaaaa gaagactaca aatcaatgat ttgtccagat tttcgataaa cgttg
//
LOCUS       DACEZN010000174         4743 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEZN010000174 DACEZN010000000
VERSION     DACEZN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.174"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(550..2163)
                     /locus_tag="IXH60_002500"
     CDS             complement(550..2163)
                     /locus_tag="IXH60_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1991387.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2304..3212)
                     /locus_tag="IXH60_002501"
     CDS             complement(2304..3212)
                     /locus_tag="IXH60_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1991388.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3395..4519
                     /locus_tag="IXH60_002502"
     CDS             3395..4519
                     /locus_tag="IXH60_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1991389.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         1608 a          801 c          853 g         1481 t
ORIGIN      
        1 aaataaatag gaacaaaaaa attaaattat ttaatacaca cattagggat cattcattga
       61 aggattagaa gacaacgagg atggcgaaaa atatgattac aatagaaaag tcgttctaaa
      121 ccaatcagtt cctagtatca cacaacgcaa gccagtcgat ttggtgagat tagaggagtg
      181 aaagcaaact ttattatata agaaaaaata ttcgaaaggg atgctagttt aggtactagt
      241 gctgatagat taatatgatg cctcgtaata ttaggcgtag ttactctaga gacatcacta
      301 tggactatga tttgtctcag agtcacaaca tgttttttta tgaaaagaaa cagctaaaat
      361 aattaaattg cgcaatgttt aaactttctt aaactataac ctaataaatg tgaagaaata
      421 atgcacaatt tcttcacatt tatcttctat tctatataca taccaaccaa caaccctata
      481 tactttttca tttttactcc tcaagtaaaa ataactccta gaccgcggct acccaccgcg
      541 gtcgtttttt tattcttcaa tcaaaatcgc tttgataccc aaacctgcca agattcgggc
      601 agaagtataa gtattcaatc ctcttctgaa agtaatgtaa acttttttat cttttgggat
      661 ttcattgatt cgatctcgta actcattcat tggaatattt tttgttgccg agatactgcc
      721 agaaggtgct ttattaggtt cacgtatatc taaaaagaac gcttctttca aatcgttttc
      781 gggtatatca cttgctttga tcgtttctac gatattcgtc atttgattga ttgcgacata
      841 accagctata ttcaacgggt cacgggtagt agaatacggt ggagagtaag gcaactccaa
      901 atcaggcaaa tcaaagactg tcaagtttcc ggtgattgct acagaaagtt ctcccatccg
      961 tttatcgaca cctttttctc caaatgcttg tccacctaaa atacgaccgc tatctgcatc
     1021 aaaaatcagc tttagattta atcttgtagc ttctggatag aaataggcat ggtcaaaggg
     1081 agtgataaaa acagttttat aatttgttat gcctgatgct tttaattcat gttctgtcat
     1141 tcccgcataa cttgctgtat gatcaaagat ttttgcgaca cctgcaccaa tatatccgcg
     1201 atagcgcata ggcgtaccat ttaaaatatc tgccagcata tgtccttgac gattggccgc
     1261 actggaaagc atactttgga tcggctgacc agtcactaca ctagtcgttt cgataatatc
     1321 gccaatcgca tagatgtccg gcagattggt ttgtaactgg tcatcaacaa tgatctgacc
     1381 tgtatctgat aattgtatgc cggttgcttt caccacttca ttcttcggag aaacaccaac
     1441 agcaaaaatt agcatatcag cagaaaggac ggagccatct gataatacta tttctcgacc
     1501 tttatcacgg atctcagtaa ctcttgtttc taaatgaacg gctaatcctt ctttcagcaa
     1561 tttgtcataa actaaattag caatttctgg atcataaggg aaagcgactt gtggcaattg
     1621 atcgattaaa gtcacgtcta agccgcgatg cttgaaattc tcagctaatt ccagacccat
     1681 aacacctgca ccaagaatga cgacatgttg tggatttttt tcttcaagga acgatttgat
     1741 tcgatccgca tccgtcacac tacggaggac aaagccgttt tctgcttcaa aaactccggg
     1801 aatatccgga taatctggtc tagcaccaga agagatgatc aacttatcgt aatctgtttt
     1861 tatttcttca tttgtggata ggtctttaac tgttaatcgc ttagtagaag gatcgattgc
     1921 tgttacttcg tgtttagtaa agacatcgat gttgtttttt gttttcaata tttctggtgt
     1981 acgttcgatc agcgagtcac ggtcagtgat cacacctcct aaataataag gcaaagcaca
     2041 acttgcataa gaaatatttt ctccgcgttc atagataatg atttcgtgcg cttcattcaa
     2101 tcgacggaaa cgagtggcaa atgaaggacc gccagctaca ccgccaatga caacaacttt
     2161 cataaaaaca tctccttttc tacgttgatc gtactatgtt atggtaaaaa acagcaaata
     2221 attgtgccgg aaaattattg tttattttga atagacagaa aatatagaga aataatttgt
     2281 ggataaaacc taaaaatttt tttctatttt aaggatagat aatactttga aggggataac
     2341 cctgtatatt ttttaaaagc tgaagaaaaa tgatggatcg aatgaaatcc taatatatct
     2401 gcaatttctg tataggttaa ttcattttct agcacgagac gttttgcttt ttccattttt
     2461 agttctttaa aatagctaat aacacttttt ccattcactt ttctaaaatg tttttttaag
     2521 ctggctggac tgataaaaaa aatttgggct aattgttcaa gcaaaaaatt ttctgaaaga
     2581 tgttcttcta aatatcgtat gattggatca actaattcat ctgaataaag aatatgcttt
     2641 tttttaggaa tgactttctc atttgaaggt attgtcaata aagcaagttc ctgttgataa
     2701 tgccttggta aggaagacaa ttgaattttt tgcagacaat aagtaactaa taaggaaagc
     2761 aaatgaatgc tcatctgttg gcatagtatg aaaaaagaat tgatttcctg atcataggaa
     2821 atagctggct tactatgttt tagataatca attttgaact gttcattttt cagaaaagta
     2881 atgatagatg ttatttcttg atctatagaa aaagattgtc ccgtaatatg tgctaataat
     2941 tgctcatcaa aataaaactg gataatgata aaagtttgtg tttccttttg agtagaaaag
     3001 atttggactt cttgctgttt atcaatgaat aaaagggaag gattatctag ttctatagat
     3061 ttatgtgcaa attcagcata taaattagcg ctttcattta ttagtagtcg aaaataggga
     3121 gcagcagata aatgaagatg ggtatctatg gaataggtta gtgttttttt gacaagattg
     3181 gaggattggt tgtttgttaa caaaagaacc actttccttt ctctttttat ataaaaagct
     3241 ttctatttat aattaatgac tattataaca aaaatgaaaa gccaaaacga caagtaaaat
     3301 atttttttat ataaatattt gtgttactaa ttccgttaat atacataagt gaaagcgttt
     3361 tatataagaa cttgaaaaga aagaagggat agacatgaaa aaaagataca aattggtgat
     3421 agctttgcta aacattaccc tattattcag cttaagtgca tgtggaaaaa ctaacgaaga
     3481 agtaacagca aacatcgcag atccaatgat aaaaaaagcc actacattag aagtatatgt
     3541 cgcaaacggc aaactgacaa actatgttga aaatgctgca aaaatctata accgcaaaac
     3601 tggagcaaat attgacttaa aaattgtgaa tgtagcttcg ggaacagcaa cagtacaaat
     3661 gatcacccca aaattggtaa cacacgaaga tatgccggat attatctcca tggcagacac
     3721 ttcagcagca ggtgtgctgg aaaaatttca agatgctttt tatagtgctg atgaatatgg
     3781 attttatgac aaatatggcg atcagtttta tgatcaaaaa ttgaatattt tgcgtcaaca
     3841 aacgagtaat gaaacgatta ttccttgggc tagtgatttt accccagcac tttgttatta
     3901 tcaaccaaaa aaatttgctg aaataggtgt agattttgaa gaaatcagtt cttgggaaga
     3961 atatatccag gtggcaaaag cattaaaaga aaaaactggt agttttggaa ttgccttgcc
     4021 agaatctggg gaccaagaat tgtttatcaa catgctagcc cagcagaatc aatctttgct
     4081 tgatgaagaa ggaaatatcc gtctgaatac taaagaagcg aaacatgctg caaagttaat
     4141 caaagaaatg atcgactcag atattgtaca tttctacggt gcacaagatg ctgagaaagc
     4201 atttcaagaa tctgcaatgt ttgttgctgg cggctggtat gctacgaata tgtctttgaa
     4261 ttttcctgat gcatctgaca aatggcgcat ggctccactg attccttttt caaaagaaaa
     4321 tccaggaaaa ggtgcagtat caggaggatc aagtttttat gtccctaaag atgccaaaaa
     4381 tccacaagtg gcacaacaat ttcttacatt tatgctgaca gatgaagaat gcttagccaa
     4441 cgcactggag ctgggcgtgg ctacatctaa ctcattggcc tatcaaacag aagcagctga
     4501 gaaaaagttt gactactaag gaggacaaaa atactatgaa ctattgaata aaatcaatga
     4561 aaatacatca aatactgttt taccagccac ttattcagat gctacgtctt atttgactac
     4621 cgctgcatat aactattggc aaacaggtga ttttgaaaat tcatatctaa aagaagcaga
     4681 aaattttgct cataagtacg gcgtaaaatt aactggtgat taaaaaatat taggaggact
     4741 taa
//
LOCUS       DACEZN010000175         4727 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEZN010000175 DACEZN010000000
VERSION     DACEZN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.175"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            221..655
                     /locus_tag="IXH60_002503"
     CDS             221..655
                     /locus_tag="IXH60_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1991390.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="IXH60_002504"
     CDS             678..998
                     /locus_tag="IXH60_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991391.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="IXH60_002505"
     CDS             1139..1330
                     /locus_tag="IXH60_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991392.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="IXH60_002506"
     CDS             complement(1401..1658)
                     /locus_tag="IXH60_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1991393.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="IXH60_002507"
     CDS             1816..2079
                     /locus_tag="IXH60_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991394.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="IXH60_002508"
     CDS             2103..2291
                     /locus_tag="IXH60_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991395.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2374..2559)
                     /locus_tag="IXH60_002509"
     CDS             complement(2374..2559)
                     /locus_tag="IXH60_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991396.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            2774..3145
                     /locus_tag="IXH60_002510"
     CDS             2774..3145
                     /locus_tag="IXH60_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1991397.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3295..3367)
                     /locus_tag="IXH60_002511"
     tRNA            complement(3295..3367)
                     /locus_tag="IXH60_002511"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Gln,seq:ctg)
     gene            complement(3413..3841)
                     /locus_tag="IXH60_002512"
     CDS             complement(3413..3841)
                     /locus_tag="IXH60_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991398.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(3901..4659)
                     /locus_tag="IXH60_002513"
     CDS             complement(3901..4659)
                     /locus_tag="IXH60_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1991399.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1651 a          736 c          924 g         1416 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
     2341 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
     2401 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
     2461 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     2521 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     2581 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     2641 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     2701 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     2761 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     2821 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     2881 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     2941 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     3001 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     3061 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     3121 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     3181 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     3241 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     3301 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     3361 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     3421 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     3481 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     3541 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     3601 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     3661 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     3721 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     3781 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     3841 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     3901 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     3961 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     4021 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     4081 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     4141 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     4201 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     4261 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     4321 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     4381 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     4441 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     4501 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     4561 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc
     4621 ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct
     4681 gtttatcttt cctattataa atgtattttt attggaaatg gaagtat
//
LOCUS       DACEZN010000176         4652 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEZN010000176 DACEZN010000000
VERSION     DACEZN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.176"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(12..482)
                     /locus_tag="IXH60_002514"
     CDS             complement(12..482)
                     /locus_tag="IXH60_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1991400.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IXH60_002515"
     CDS             complement(641..1099)
                     /locus_tag="IXH60_002515"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1991401.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2414)
                     /locus_tag="IXH60_002516"
                     /pseudo
     CDS             complement(1270..2414)
                     /locus_tag="IXH60_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2427..3344)
                     /locus_tag="IXH60_002517"
     CDS             complement(2427..3344)
                     /locus_tag="IXH60_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1991402.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3772..4335
                     /gene="efp"
                     /locus_tag="IXH60_002518"
     CDS             3772..4335
                     /gene="efp"
                     /locus_tag="IXH60_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1991403.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1453 a          846 c          877 g         1476 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatctttt ggttagaagt cactaaacca
     1801 gctactacat cgctgtgtcc gcctagatat ttggtaccgc tatggacaac gatatcagaa
     1861 ccgagatcta aaggtctttg aaaataagga gtagcaaatg tattgtcagc aataactatc
     1921 aatccatgtt gatgagccag agtggcactt ttttctaaat cagtaatctt tagaagcgga
     1981 ttgctaggtg tttcaagata aagtgctttt gtattgggtt tgatggattg ttctatttta
     2041 tcaagattac tcgtatctat gatcgtgtac tcgagaccat ttttagtaag aactttgtca
     2101 aatagacgga atgttcctcc atagacatca tctcctaata agatatgatc gccagcttga
     2161 aaaagagaaa aaacggcatg aatcccagaa agaccagaag aaaaagcgaa tcctcgaaca
     2221 ccgccttcca aatcagcaat cagttcttcc aatgcaaaac gagtaggatt accagatctt
     2281 gaatattcat attgtttagg ctggccgacg ccatcttgcc gataagtgga tgtttgataa
     2341 ataggtacac tgacagctcc tgttgtggga tcttcactga tgcctccgtg gatcaatttt
     2401 gtttgaatgt gcatgtttct ttcctcctat aagtaaatgt tttttgataa atagcggtct
     2461 gctgcgtctg gaaaaatcgt aacgacacga tgaccaggtg gtaaacgacg aatttctctt
     2521 aatgctgctg caaaagccgc accactcgaa cttccaacta atagtccttg ttctcgtgct
     2581 aactgcctag tatagttaaa cccttctaca tcagagattg tctcgatctg attgattgaa
     2641 aggggagaaa gaaacggcgg aatgaattct acaccgatac cctcaatttc gtgaggggct
     2701 ggatcaccac catttaacac agagccctct ggttctacac cgattattcg aatgtcggga
     2761 tatttttctt tcaaaaaggt agaagttcca gcaaaggttc caccactccc aattcctgct
     2821 acaaaactat ggatattttc tttgatttca tgaaagattt ctggccctaa cgtatgatag
     2881 taagcagcag gattatcttt attttcaaat tgaagaggca gatagctgtt tgatatctcc
     2941 tctgcaagtt ttttactttt gctgatggca ccgatgattc cttgctcact aggagaatgg
     3001 atgatctttg ctcccaatgc tttcatcaat tgttgttttt ctaagctaaa cttctctgga
     3061 acaacaaaaa ccgttcttaa tttgtatgtg agtgcggcca gagcgatccc aattcctgta
     3121 tttccggcag ttggttcgat gatcgtcgtt ttttcattga tttttccttg ggcaaatcct
     3181 tctttgatca aataactgcc caatctatct ttgatacttc ctccaggatt taaatattct
     3241 agttttgcat aaatagcaga acctttaggc atagaatagt gctgattgtc aaagcgataa
     3301 agcggtgtgg aaccaatagc ttctgtaatt gtcgagataa tcatgtgggc ctcctaaaaa
     3361 aagataataa aaaaaggagc tgactattaa atcagctcct tgaaagacat agagaaaaat
     3421 tgagtagata aaaagataag ttacctggta ctcattctat gaagggcaat taatagacat
     3481 gacaaatagt atgctgcttt tatgcaccat acaacagcaa cgattcaatt gtttttggtt
     3541 gctgactgtc atgtgtatcc ctccaagtaa aatttttatt aacgatagca tgaaaaaaaa
     3601 taaaagtcaa aggcttgaaa gaaaaaaata tttcgatagc atcgtttacc cgtgtttatt
     3661 tttctgacaa ataggcatga gaccttaaaa gatagagaat actctagttt tctggccggt
     3721 tgtctgatac aataaagttg attatgataa agaaaagaag gagcaaaaat aatgatctca
     3781 gcaagtgatt taagagccgg tatgactttc gtacaagacg gcaaactgat taaagttctg
     3841 gatgcaagcc accataaacc tggtaaagga aatacagtta tgcgtatgaa actgaaagat
     3901 gtccgttcag gtgcaacata cgacacaacg ttcagaccag aagaaaaatt tgaaaaagca
     3961 catatcgaca caaaacctgt acaatatttg tacacgatgg atgatacagc tttcttcatg
     4021 gatttggaaa catatgagca atatgaaatt cctgtagaaa cagtagaaga agaaatgaaa
     4081 tatatcctag aaaacatgga agtaaaaatc caatttttcg gtagcgaagt catcggcgtt
     4141 caattaccaa caactgtcgt attacgtgtt gtggaaacac aaccttccat caaaggtgca
     4201 acagtcactg gttcaggtaa gccagcgaca atggaaactg gcttagtagt caatgttcct
     4261 gacttcatcg aaacagatga attgttggaa atcaatacag cagaaggttc ttatgtaaaa
     4321 cgtgcgtcaa aataattcgc taacggattg ataagaattt gaaaacagag cttgacctct
     4381 tgaaaggcag atcatcggat ctgcctttca agaggttttt tgtggaatca gcaaattgaa
     4441 aaataaaaaa gtatttgatt ttatttatat tttgaaagat gaatggcaaa agctagagaa
     4501 agaaaaaact gcacttattt atgaagaaat tgaaatgatt cataaaattg aaaatatccg
     4561 aactattccg aactatattg aagcgaaaat attttttcat cactaaatag tgttgaattt
     4621 atgtttttat gtataaatag cttctaatag tt
//
LOCUS       DACEZN010000177         4645 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEZN010000177 DACEZN010000000
VERSION     DACEZN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.177"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(45..1187)
                     /locus_tag="IXH60_002519"
     CDS             complement(45..1187)
                     /locus_tag="IXH60_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1991404.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(1238..2035)
                     /locus_tag="IXH60_002520"
     CDS             complement(1238..2035)
                     /locus_tag="IXH60_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1991405.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            2237..2536
                     /locus_tag="IXH60_002521"
     CDS             2237..2536
                     /locus_tag="IXH60_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1991406.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            2801..3676
                     /locus_tag="IXH60_002522"
     CDS             2801..3676
                     /locus_tag="IXH60_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991407.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            3673..3891
                     /locus_tag="IXH60_002523"
     CDS             3673..3891
                     /locus_tag="IXH60_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991408.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            3878..>4645
                     /locus_tag="IXH60_002524"
     CDS             3878..>4645
                     /locus_tag="IXH60_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991409.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNL"
BASE COUNT         1591 a          618 c          813 g         1623 t
ORIGIN      
        1 atttgtattt taatggttgg ctacgttttt ggctacgttt ggaattattc gcccataaat
       61 tttgcaaatc ggtcaccagt tttcttaaca gattgtggtg tgacatgagc atagatattc
      121 atggtagttt taatatcttt atgacctaat cgttcctgta cttcttttat cgacgcacca
      181 ctttcaaaca ataagctaca gtgggtatgc ctaaatccat gtggagtaat ttgaggctcc
      241 atcggtgtct ttttgtaaat ccagtctaac caatcgttta ctacttgggg gtagtacaac
      301 ttgtttgtgt ttgtagtgaa aagaaactgt tttggtgaat tcgtgttata gccatataag
      361 atcattattt tcatttgttc ttgttgccaa atacgcagtt gtttaagtgt aaaatcatca
      421 agctgaatcg ttcttgatga atttttagtt ttaggtactt gtagaactac ttgattaaac
      481 tcgtcttgag cgatagtttt accaattgtt attgttttat tgaaatagtc tatatcttcc
      541 cattgaagcg ccaatgcttc gcttttacgc attcctgtat aagctaataa acggaaaaaa
      601 gtgtacagtt taactgaatt attttcttta gtattctgta aaaacagttg taattgatct
      661 ttcgtgtaaa acttcaaggg ttgttcttct tcaatttttc ttggaagcaa tgttttagac
      721 ataggattat ccttacagac ttccattgct actccaaatt taaatatttg agcagttgtt
      781 tttctcaaga aaccatattg ttttaatgtt ttgtgccatt cattcacctg tttttggcaa
      841 tagctaacac ttattttatc caatcgtaat tccccaaaag caggtaatat atggtttttg
      901 caaaaacgtt gagcaacggc aacagaagac ggttttactc ttgttctgta gttatcaatc
      961 cataattcat acaactcttg aaaagttgta acattgattt taaactcttg tttttcttta
     1021 aagtctacaa gtagtctatt aagtgctgtt tgcgcctcag cttttgattt gaatcttcta
     1081 cgctttgtac gtatatcttt tccagtcttg ggatctttcc ctaaataagt ttcaaatccc
     1141 caatattttt cgccattttg taatttataa ctaaaaaatg ttgccatttg attctctcct
     1201 tacttcatta ttgcaggtag agaagttatt tgaagaatca attattttta aatttatcgt
     1261 gattatcagt taaaaatttt gcgattatac tatttttctc atatgggaaa ttgtattttt
     1321 tactaagatc tgaaataaat ttttctgttt caaattggtt tttagtaatt ttataaaaga
     1381 aatcctcgac agatgaatac tttgaaatat ctttataggc taagtccatt tcgtctaata
     1441 gctgttttaa agaatcaatc attctttctt tttcaccgta tttgaaatca ccatttctta
     1501 tcttgtcttg aagtttactg ttggctcttt tgaaaaatag ctctttagag atagagtgtc
     1561 tttggttagc gtactcttca atcagaggat tgatattatt agcaagtatc tcatttagat
     1621 aaatttcaaa atcttttgag taatattttg taaatatatt ttttattata ggattcaata
     1681 actcatagtc tttatctagc gaaaacgtat cactatatct ttcttgaatg tctttaaaga
     1741 ctttgtgagg tatttctggg aagtccttaa tatatagctc ataccctata tcgataaggt
     1801 atgaagttat atattcttct aaagttcccc atttaatcca gtcaattgtt gaattagtga
     1861 tagttgataa taatttcagc ttttgtggat ttggtaaatt ggtcccacgt tcccagttgg
     1921 atatggtcgc acgtggtgaa ttatctattt tccttccaag ttcttccata gtaaaaccac
     1981 atgaaaggcg taattgtttg attcgttttc ctgcttctga aggatttggc ttcatctttt
     2041 ttctcctttt tatttttaaa gatagtatat catatttata aaataaaagt atttaaaagt
     2101 atttactttt tatctggagt atgatacttt attcttataa atacaaataa atacattttt
     2161 ttacgcaatt tgtatttatt tgtacttata aaggtagagg agttatagaa taattaatta
     2221 aaaaggagag gtttttgtgg aagcaataaa attaatgatt tcagaagaac aggaggaaat
     2281 gtttctaaag cagttttatc aaattgctac aaaagctatt gaaaaggctc aagaaaatgc
     2341 ggggatgaca agggaatatc tcaatcaaaa agaaatggcg gaatacttaa atgtgagtga
     2401 aaacttttta aaaaatgctg tgagaaatga gggattgccc gtagttgaat taggggcaag
     2461 aaaattttat tcgaaaaagg cagttaatga gtttatgttt gctcgaatga aaagagggga
     2521 aaatattgga agatgatgaa gtagaaatta taaaaaaatg ctttaaaaaa agaaaaaaag
     2581 tgttcccaaa agtttgcaga ctgggggaac actcaataat aaagtgctga gaaaacttta
     2641 tttatagcta ttatatcaaa aaagaaggtg aaaaggtgtt ttcttctgga aatgttgtag
     2701 ttgacaaact tagagaaata aattttgacg gaaatgtaat ccacacacat ggtacgatca
     2761 attaagaaaa aaaacgaaaa aaggtgttga gaagccctat ttgaatgcaa tagttatctt
     2821 agcagaaatt acctattggt atagacctaa aaaaattttt aacgatgatg gtcagctact
     2881 tggttatcaa aaaaagtttc ttgaagatat tttgcaaaaa tcatataagc aattaagcaa
     2941 aaaaacagga ttatcagaac gagctataaa agatgcaatt gtgtacttag agaaaaaggg
     3001 gattatcaaa agggttttta gaaatattta ttatgagaaa agagcagtta cattatcaaa
     3061 tgttatgtac attgttttaa atgtacataa attgatagaa attacttttc ctactttgga
     3121 ttgcgaaaaa aaggagtata tgccgaaaat tcgtcagagg ggtatcgaaa aaaagagata
     3181 tgtctcttta aaaaaaggta gaggtatgta caaatttgta tatacgtata caaagactac
     3241 ttcacagaac atatcaaaga ctactctcgt tatggaagaa gaggatatta atataaagag
     3301 gatttacagt aagattagtg ttctttatga cgcaaataac gaagatggtc gaaaattatt
     3361 tcaagtgtat cactatttga gaaacgctaa tgtagataca gaagatatca gaaaaatcat
     3421 tgaatattta tctgaaaata ctcagtatct tattgctgag tgtattataa aacaacatga
     3481 agcatgcttt tttcaaagaa aaactgaaga agggttatac gattatgtaa catatttttt
     3541 gaatggactt cagaaaagat ataaaagtca atttttagaa aatgtagagc ctatagatca
     3601 gttcaaaaaa gctttcgaaa tcaaagagtt acctgaaatt actatgcata attggttgaa
     3661 aggagaagaa tcatgattaa aaatcgctta atagatcaaa tgtggtgtct tgatcaaaat
     3721 acagatactc tcgaattaat cgagagtatc tgcttcaaca cagtagttct cgatttaaga
     3781 gctgaaaatg acaattgtgt aactcatatc ataattaatc aaaaaatggc tcagcagctc
     3841 tctgagagcc taagaaaatg ggctgaagga gaaaactatg aaagcaatta aagaaaaaat
     3901 cgttagtaga aaaataaact ggttagcact cagtgtactt gttttaatga ttagcgttgt
     3961 ttttacaagt aatattgttg aagctagcat tttcaattgg tttgatgaca caaaaagtca
     4021 aacagagttt ttgaataatt ctctttatag accttaccta aaaaaataca atggagattt
     4081 ccttagtcag ttcgggattt ggcttggatg ggctgtggta aaaggcatgt ttactgtgac
     4141 tgattcgatt caaaatatga ttccagatgt gttggatttg ttcaatttca ttgaaagtac
     4201 agggctaaat aatgtttatc aatcagtcat aaatacaatt gtcgttggat taatgatcct
     4261 ttcattgatg tttgttggct acaagatgat tactggaaaa ggaacaatag atttaagatc
     4321 tgttggaatg aatatagtga tgtctgtagc gttaattctt ttaatgccaa caatgatttc
     4381 tagcggtatt caatttgcca aaatatttta caatgatgct actacgatta caaattcaga
     4441 tgacggagtc gcttggtcat tgataaaaca aggagtaaca gatctggcat atattaataa
     4501 aacagatcaa tactcaagca ttgataaaac ggaggatcga aataagttaa cacgaaaaaa
     4561 ttttcaacag acagatttaa cacaagtgtt gacggataaa gtgattgata aattggaaaa
     4621 agaaaatcca gctgcggata atctt
//
LOCUS       DACEZN010000178         4616 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEZN010000178 DACEZN010000000
VERSION     DACEZN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.178"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(15..962)
                     /locus_tag="IXH60_002525"
     CDS             complement(15..962)
                     /locus_tag="IXH60_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1991410.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="IXH60_002526"
     CDS             complement(1140..1943)
                     /locus_tag="IXH60_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1991411.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="IXH60_002527"
     CDS             2089..3003
                     /locus_tag="IXH60_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1991412.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="IXH60_002528"
     CDS             3051..3521
                     /locus_tag="IXH60_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1991413.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="IXH60_002529"
     CDS             3606..4214
                     /locus_tag="IXH60_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1991414.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="IXH60_002530"
     CDS             4348..4548
                     /locus_tag="IXH60_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1991415.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1511 a          716 c          912 g         1477 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttag
//
LOCUS       DACEZN010000179         4600 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEZN010000179 DACEZN010000000
VERSION     DACEZN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.179"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..527
                     /locus_tag="IXH60_002531"
     CDS             <1..527
                     /locus_tag="IXH60_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1991416.1"
                     /translation="AFSGCRIGEILALTWDNINFKTNEMAIKKTVARSDKYYISETPK
                     TKKSNRIIYLDEKTIKQLKFWKLEQRKYLFQLGFTKANYLFTNDENNFTINQSVAERY
                     NIYRERAGLPYIGLHGFRHTHASMLYEAGADHKEVQERMGHANIKTTMDTYTHITNSK
                     KEETTQKLTNYINF"
     gene            complement(620..913)
                     /gene="rpmA"
                     /locus_tag="IXH60_002532"
     CDS             complement(620..913)
                     /gene="rpmA"
                     /locus_tag="IXH60_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1991417.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(926..1270)
                     /locus_tag="IXH60_002533"
     CDS             complement(926..1270)
                     /locus_tag="IXH60_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1991418.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(1297..1605)
                     /gene="rplU"
                     /locus_tag="IXH60_002534"
     CDS             complement(1297..1605)
                     /gene="rplU"
                     /locus_tag="IXH60_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1991419.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            1887..2528
                     /locus_tag="IXH60_002535"
     CDS             1887..2528
                     /locus_tag="IXH60_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1991420.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2590..4221)
                     /locus_tag="IXH60_002536"
     CDS             complement(2590..4221)
                     /locus_tag="IXH60_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1991421.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4344..>4600)
                     /locus_tag="IXH60_002537"
     CDS             complement(4344..>4600)
                     /locus_tag="IXH60_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1991422.1"
                     /translation="FKMSKEELSRKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEI
                     TSMLLISIFFVALYGSLTSDRWILLKQGGLALLALLSIFL"
BASE COUNT         1494 a          975 c          785 g         1346 t
ORIGIN      
        1 tagcattcag cggatgtaga atcggcgaaa tattagcact cacttgggac aatattaatt
       61 ttaaaacaaa tgaaatggcc attaaaaaaa ctgtagctcg ttcagataaa tattatatat
      121 ctgaaactcc taaaaccaaa aaatcaaatc gaataattta tttagatgaa aaaactataa
      181 agcaactaaa attttggaag ctcgaacaaa gaaagtactt atttcaatta ggatttacta
      241 aagctaatta tttgtttacc aatgacgaaa ataatttcac aattaatcag tcagtggcag
      301 aaagatacaa tatatatcgt gagcgtgccg gcttacctta tatcggtctg catggtttta
      361 gacatacaca tgcatcaatg ctatatgagg caggcgcaga tcacaaagaa gtccaagaaa
      421 gaatgggcca cgcaaatata aaaactacta tggacacata tacacacatt actaacagca
      481 aaaaagaaga aacaacacaa aaactaacaa attatattaa cttctaagaa agtatggtca
      541 aaagtatggt caaaaaaaat cccataattt acaaaaaagc tccatccctt gctacacaag
      601 agatagagca gtttatttat taagcttctt gagctactgg ataaacagac acttgttttt
      661 tgttacggcc tttacgttcg aaacgtacta cgccatcaac ttttgcaaat aaagtgtcgt
      721 ctccaccgat acccacgttt acacctggat agatttttgt accgcgttga cggtaaagaa
      781 ttgatccacc tgtaactgtt tgtccatcag cacgtttagc acctagacgt tttgattctg
      841 agtcacggcc gttagatgta gaaccgccac cttttttgtg ggcaaataat tgtagattca
      901 tagataacaa catggtctgc acctcctatt tatcattaat tgttttaatt tggatatatt
      961 ctgagttttc agcttctatc gattgtaatc ctaataaaag attttctaat agaatctcag
     1021 caatattgtt ttgttcttgt gtcattcctg aagtgacttc ggtataaaga taaccgcctt
     1081 cttcttcatt catttcaaca atcggctcaa aaccggctaa tgaggcaatc ccgttgacag
     1141 tgctgatcgc taacgcagaa acacccgcac aaacgatatc acttccataa ggaccagcat
     1201 cagcatgccc ggtcaaagtg aatgaaacga tctgacctgc gtcatttcgc ttaaatgttc
     1261 ctttgatcat atataggcct tctttctcaa acgctattaa gcgttgatcg cgtcgataac
     1321 cacttttgta tatggttgac gatgaccttg tttacggtgt gaatgttttt taggtttgta
     1381 tttgaaagta acgactttct tttgtttgcc gtgtttttca acagtacctt caacagttgc
     1441 acctgcaaca gttggagcgc ctacttttgt agattctccg cctactaaga taacttcgtc
     1501 aaaaacaact ttttctcctg cttcaacgtc aagtttttca acatagattg cttgtcccac
     1561 ttcaactttt acttgtttac caccagtttt aataattgcg tacatgctat aaagcacctc
     1621 cttctgatat acttagactc gccatccgaa gtgacagcat atgctgtgct tattacttgt
     1681 tctgagcggt tgtagctgtg gagtccacaa ttacaacagt actagcttac cagaacagca
     1741 accgttagtc aactaaattt aaaatcaaaa agtaaaacaa aaattcaagc aatacttctg
     1801 cttttctatc ctcacagttc taccaaataa aatagtcgca ttgtgtttct tttattctta
     1861 taataaaaga aaaaggagga gcataaatgg aatacccaaa agaactgata caagaagctt
     1921 ctttaagaat gaacggccgt cctgtagaag gatttatcgc tgggcaagga cctcttcatc
     1981 ctaaactgat gctcgtggga gaagccccag ggaaaacaga gattgatacg catgttcctt
     2041 ttagcggtca agctggaaaa gaattgatgc aggcactttc ttcaactggc ttaacacgag
     2101 aagaggtgta tatcaccagt gctgtaagga gccgcccgta tcgcgtgact catcggatca
     2161 ataaacggac acaacagtca gaaactgttt atccaaatcg gacacctact cgttccgaag
     2221 tatttgcgca tgcaccaata ttggattacg aactgatgaa ggtccagcca aaattaattg
     2281 cgacacttgg aaacattggt cttcaacgtt tacttggcaa agaatatacg gttaccaaaa
     2341 atcatggtca actctataca gggcctgtct tacaattaac agaacaaaag gatgcctacg
     2401 aatggtcaga aaaaaactat cgaattcttc cactttttca tccagcagct attttctaca
     2461 atcgctccct tgtacctgtt atccatcaag actggcaagt actagcttca cttttacagc
     2521 aatattaaaa agaacagaca agaccataaa atcttcagtt tacagtcttg tctgttcttt
     2581 ttcattgttt taatggctaa ttgcagtata tttcgcatgt aaagctgtca acgcatgagc
     2641 aactgtttga tgttgatgcg tgaatgtatg ttcccacaag tgagaaagtt gtttttctac
     2701 ttctggctgc aaggaagtat agataatcgt ttgtcctctt tgctctgctt tttcttgaaa
     2761 ttcatcaaaa actgcacgtg ccgtcaaatc aatacttggt acgcctcgca tggagaaaat
     2821 gacaccatcg tgatgattta gttcttccaa tttatttttg agtttttctg aagacataaa
     2881 aaacagtgga ccactaatat acactactgt ccagttattc agcttttccg tttctggcag
     2941 ccccattctt tgccaatcga tcgtttctaa tgaaatcgtg atagcagcgc ttttgacaac
     3001 aaaaaataca cagccgccga tcaccccgat cacgatagca atacttaaat caaagataac
     3061 tgtacaaatc atcgtaagga taaacaatag aactgctgac caatatttat tagtaaatag
     3121 ctccttgatc gtttcccatt cattcatacg ccaagccgtc acaatcaaaa cacctgcaag
     3181 tgctggcatt ggaatctgtg acatcactgg cgcaaaaaca agcatggaga gcagtaagac
     3241 aactgcatgt atcataccag ctaatctagt ttgtgcacca gatttgatgg ccacgcttgt
     3301 acgggcgatg gccgcagtag caggaatccc gccaaagaaa ggaagcaaca gatttccaat
     3361 tccttgcgcc attaattctt gattgctatc taagggctta tttgccattc ggccagccga
     3421 agcaccacat agcaagcttt caatcattcc taataaagca atgctaatag ctggaactgc
     3481 cacttgttgc aaggcagaaa agctaaaatc tcccatattc aaacgattac tactgatcaa
     3541 tgtttgtgga atcttaccca cagttgctac tggtaaatgg aacaatagca taactgctgt
     3601 tgccaaaatg atagccagca gcgaactagg cattcgtttc gcccattttt ttggatagat
     3661 aaacatccct aaaatgacta gccctcccat gatcaaagag gctgtgttta tcggaaatcc
     3721 tatcgtttga taactactta gtttttcaat aacgtttgta ccttccgagt gaacaccaaa
     3781 taaattatcg atctgaccta atgcaataat gatagcaata cctgaagtaa accccgtaat
     3841 tacgggtgct gggataaaag aagtcagtgt tcctaatcgg aaaagtcctg ctgcaagtaa
     3901 aaatagaccc gctaaaaaag tagcaagcaa tacaccttgc attccctttg ttgccgcaat
     3961 agacatcaag atagctgcca tcgcacctgt cggacctgat atttgataaa aaccgcctga
     4021 taaaccgcca ataacgatac cggcaatgat agccgtaatc aaacctgcgg ccgcatcagc
     4081 tccgcttgat accccaaaag ccaaggctaa aggcaatgct acagcagcta ctgtgatccc
     4141 agccaaaaga tctttctgaa atcttgaaag atcataacct gaaaattctt tttttagtga
     4201 ggaaacataa tttttgaaca cctatctaac tcctttttca tgaaattttc taaaatacca
     4261 ttcattagaa tatcatattt catgagataa agtaatattt ttcacaaagt ttcctgttag
     4321 ataaattctt attttttcta tatttataag aaaatagaaa gcaatgccaa cagagctagc
     4381 ccaccttgtt tgagtaaaat ccaacgatcg ctagtcaaac tgccatataa tgcaacaaaa
     4441 aaaatagata ttaatagcat acttgtgatt tctttagatg cggaagaaag ataagctcca
     4501 taaagtagtc ccactcctaa tagtccattg tacactcctt ggttcttaaa caatgtttga
     4561 acagattttc tgcttaactc ttctttactc attttaaaaa
//
LOCUS       DACEZN010000180         4464 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEZN010000180 DACEZN010000000
VERSION     DACEZN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.180"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IXH60_002538"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IXH60_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1991423.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IXH60_002539"
     CDS             complement(3250..3768)
                     /locus_tag="IXH60_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1991424.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1460 a          732 c          679 g         1593 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggt
//
LOCUS       DACEZN010000181         4433 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEZN010000181 DACEZN010000000
VERSION     DACEZN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.181"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(543..2048)
                     /locus_tag="IXH60_002540"
     CDS             complement(543..2048)
                     /locus_tag="IXH60_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1991425.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IXH60_002541"
     CDS             complement(2045..2731)
                     /locus_tag="IXH60_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1991426.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXH60_002542"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXH60_002542"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1991427.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACEZN010000182         4429 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEZN010000182 DACEZN010000000
VERSION     DACEZN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.182"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..159)
                     /locus_tag="IXH60_002543"
     CDS             complement(<1..159)
                     /locus_tag="IXH60_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1991428.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKG"
     gene            complement(389..1207)
                     /locus_tag="IXH60_002544"
     CDS             complement(389..1207)
                     /locus_tag="IXH60_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1991429.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1368..2057
                     /locus_tag="IXH60_002545"
     CDS             1368..2057
                     /locus_tag="IXH60_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1991430.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2071..3573
                     /locus_tag="IXH60_002546"
     CDS             2071..3573
                     /locus_tag="IXH60_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1991431.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3586..4062
                     /locus_tag="IXH60_002547"
     CDS             3586..4062
                     /locus_tag="IXH60_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1991432.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4159..4269)
                     /locus_tag="IXH60_002548"
                     /pseudo
     CDS             complement(4159..4269)
                     /locus_tag="IXH60_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1474 a          641 c          789 g         1525 t
ORIGIN      
        1 accctttata gaaaacattc gcttagcaat cttaataatt ccttgtgccc gatgtcgttt
       61 agattcagta ggttcctgct catcgatcat ggaaaaaatt tgaaggatca gatccttaat
      121 aaatttattt aagagaggat gatccattgc ttcatccata ctcgataaac tagtgataat
      181 gagctacacg tttttctttc aagttgatca atagcttcca cgataaaaat atctttagat
      241 aaaatcaaga accgtaggaa ctacagggat atttggtcaa taagagccac cgctattagt
      301 aaaaaataag ctatcaagta tgctctcatc ccaagaattg cctacttcaa tcgttaaaaa
      361 ccgttaggtt tagctaagtg ggggtagttc actcaatact tttatcaata atagagcgca
      421 aaactttttc tcttaattca actgaatcaa tattattgtt ttgttcataa ccatgaacta
      481 atagatcaca aatataaagt tgtgatattt ttccagctaa agatccacca ttaagaaatt
      541 cttcaatggc tgtttgtaaa actaagtctg cagactttcc tataggtgaa tgaatatagt
      601 tagttatagc aataattttt gcgccatttt ttttagctat tttcaatgaa tcataagtat
      661 ctttcgtttt cccagataaa gaaaaaatga ttactaaatc attaactgtt aataaagaag
      721 cgacttgggc ttgaaaatga gggtctagta cagcttttgc ttgaactcct acgcgaagaa
      781 acatattttc taaatcaaga ctagtatttc cactagatcc cactccaaat atatatatgt
      841 tttttgattg agtgatcatt ttaatggctt cattcaattt ttcttcgtct aaaagcctaa
      901 tagtggaata gagagcttct gttaaactat tagcaatttc atcatagtac tttacactag
      961 tcggatgttc ttttttttga ctaaagtctt cctttgcaat ttcaattttt aaatcagaaa
     1021 aaccagaaaa accaattttt tgacaaaagc gaataatagt agcatcgcca acttttgtgt
     1081 ttttcttaat atcactcatc gtactataaa taatagtttt cccagaattt aaaataaaat
     1141 cagcgacttt tttttcagac ttcgttaaaa atggataatt ttctttaatt aaatagatat
     1201 aactcataca aactcctcta caatttcaac ttttctccat tatactccaa aataataaaa
     1261 aaaactccta aatactccat ttttatcttt acttttatga aagcggatgc tatattgttt
     1321 ttggagttgt tatccaaaaa taataaaaaa ttggagtgtt taggataatg gataaaaaga
     1381 ggtttttaga tcttatttca aaaggtttga ttgtttcctg tcaagcatta cctggtgaac
     1441 cattttacaa agaggaagaa agtttaatgc cttattttgc tttggctgct caaagggctg
     1501 gagcagttgg tatacgtgca aattctgttc gagatattat tgaaattaga aagtgtgtat
     1561 cattaccaat gattggaatt attaaaagag attatccacc agaatctcca ttcattactg
     1621 ccactatgag agaagtagat gaattagtag aaaccggtgt tgaggtaatc gctcttgatt
     1681 gcactcttag aaatcgacat gatggactat ctataaattc ttttattact aaaataaaag
     1741 aaaaatatcc tcatcagtta tttatggcag atatttctac atttgaagaa ggcaaaaatg
     1801 catataaagc aggaatagat tttgtaggaa ctactttaag tggatatact gaggaaagta
     1861 aaaaacaaga tgggccagat atagatttga ttaaaaaatt ggtagaagaa gggatttgtg
     1921 taattgctga gggaaggatt catactccag aacaagcaaa agagataaaa aaaataggag
     1981 ttgcaggtat tgttgtgggt ggtgctatta ctcgaccaca ggaaattgca tcaagatttg
     2041 ttaaagcatt aagttagggg gagaaataaa atgtttaaaa aattttcaca attaggacgc
     2101 gctttcatgt taccaattgc tatattacca gtagccggat tactattggg actgggagga
     2161 gcgttaacaa atgagagtgc tataaatgct tatccatttt tagatcaacc gtggttgcat
     2221 acaattttaa gtattatgag ttatgctgga aatgcagttt ttgcaaacct agcactaatt
     2281 tttgcaattg gtattgctgt aggacttgca aatggagata aaggaacagc tggtttagct
     2341 ggtggagtag cttatctagt atatactgca actattagtg ggtttcttga acttttttca
     2401 gcaaaagatg caacactaga tacaggtgta gtgggatcaa ttgttgttgg tggtactgtt
     2461 gcatttttac acaatcgtta tagaaaaatt gaattaccac aattcttagg tttttttgga
     2521 ggatcaagat ttattccgat tgtttctagt ttagctgcaa tcattattgg ttctttcttt
     2581 tatttaattt ggccaccaat tcaaaatggt ttagtagttg ctggtgaaca aattgcccaa
     2641 atgggtagtt taggaacttt tttatatggt tttttattgc gtttaacagg tgctgttgga
     2701 ctacaccaca ccatttaccc tttgttctgg tatacatctt taggtggtac cgaaactgta
     2761 gctggatcaa caattgctgg agcccaaaat attttctttg cccaactagc tgatccaaac
     2821 cacactggtc tgtttactta tggtactaga ttttttgcag gaagatttgc aacaatgatg
     2881 tttggattac cagcagcatg ttatgcaatg tatcgtgcaa ttcctaagaa aaatcgtaaa
     2941 aagaatggtg ggctttattt tagcggggcc cttacatcat ttttaactgg aattactgaa
     3001 ccagttgaat atatgttttt atttgttgct ccttggcttt atgtgattca tgcattctta
     3061 gatggattat ctttttattt tgctgatatc ttaaatattc gtgtagggaa ttccttttca
     3121 ggtggtttga tcgacttttt attattcggt gtacttcaag ggaatgacaa aactaattgg
     3181 ttaaaattaa ttccttttgg tattgcatgg gcatttatat atttctttgt ttttacattc
     3241 tttatcaaaa aatttaaggt agctattcct ggaatggaag aagaatcaga agttcattct
     3301 gaaaaagtaa taaacaaaaa tacatcatta catgaagagt ctcttatcat tattgaagct
     3361 cttggtggaa aagaaaatat tgagtctgtt gaagcttgtg caactcgttt gcgtgttgct
     3421 gttacgaatg gggagattat tcaaaaacaa acaataaaag acactggtgc aactgctgtc
     3481 ttcgaagtga agggtggcat ccaagctgtt tttggaggga aagctgactt attaagtcaa
     3541 gaaatcaatc aaatattagg aaaagataat taggaggttt ttaatatgtt tgaaattttc
     3601 aaaaagaaaa aacaagaaac tttttatgca ccttgttccg gaaaaatcat ttccatcaca
     3661 gaagtaaatg atcctgtctt ttcacaaaaa ttactaggtg atggttttgc cgtcatacct
     3721 gatggttcaa aaatttatgc tccattggct ggtcgagtaa caaatatatt tcctactaaa
     3781 catgctatta atataaaaag caactcaggc attgattatt tgattcatat tggaattgat
     3841 acagttgaat tacaaggaga gcctttttcg atttgtgttc aagaaaatga tgaggttact
     3901 cctgaaacct cgttggttga tgtggatttt gacaaaataa taaaatctgg taaagatact
     3961 tctgttatag ttgtttttcc ggagaaaaat cagttcaatg atttaataat agataatggt
     4021 cgaattgctc atgggaattc atgtggaaaa atcacttttt aaaaaagtgt atataaaaag
     4081 ctataaaata agctaaatag gatatttgtc aaatgaagtt ggtgaacaga aagaactgtt
     4141 ttctttctgt tcattttttt attttattag taatgattcc ccatgccaaa tcatgatccg
     4201 taatttaaag ttataaaagc ttcgaaatcc attggcattt cgttggaata ccttgatatg
     4261 attattcaaa tataaaatat agagagaaga gagagaggga agatatttat aaagggcaaa
     4321 gttatgttta ctcaacttag gaaatagaaa tagatcaatt tatattctct tgtcaatcga
     4381 atatgaacct tagcactata taccaaatat agtaaaatgc tgaaagatg
//
LOCUS       DACEZN010000183         4420 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEZN010000183 DACEZN010000000
VERSION     DACEZN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.183"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1598)
                     /locus_tag="IXH60_002549"
     CDS             complement(<1..1598)
                     /locus_tag="IXH60_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HAQ1991433.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERL"
     gene            1744..2319
                     /locus_tag="IXH60_002550"
     CDS             1744..2319
                     /locus_tag="IXH60_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1991434.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            2533..3228
                     /gene="vanR"
                     /locus_tag="IXH60_002551"
     CDS             2533..3228
                     /gene="vanR"
                     /locus_tag="IXH60_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1991435.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3206..4360
                     /locus_tag="IXH60_002552"
     CDS             3206..4360
                     /locus_tag="IXH60_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1991436.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1417 a          822 c          858 g         1323 t
ORIGIN      
        1 tttaaccttt cattaaagct gctggttctc tccgttatat aatcttcgaa tgataaacta
       61 actgataatc tcgtattccc cttcgattga ttccatgtat cttccgaaaa caaatattcc
      121 tcaaaatccc tatattgtct gctgccaaca atggaaacat ctcctgcccg aacatgctcc
      181 cgaagttctg ttaaaacagc catttcatag taatgacgat taattgttgt accatcatcc
      241 tcgtataaat gtcttttcca tcgttttgaa ataaaatcca caggtgagtc atcaggcact
      301 tttcgctttc cagattcgtt cattcctcgg ataatctcaa cagcttgtaa aagtggctca
      361 tttgcctttg tagaatgaaa ttccaatact cttaatagcg ttggcgtata ttttcttagt
      421 gaataaaacc gtttttgcag taagtctaaa taatcatagt cggcaggacg tgcaagttcc
      481 tgagcctctt ctactgaaga gacaaaggta ttccattcaa taaccgattc taaaacctta
      541 aaaacgtcta atttttcctc tcttgcttta attaatgctt gtccgatgtt cgtaaagtgt
      601 ataactttct catttagctt tttaccgttt tgtttctgga tttcctcttg agccttacga
      661 ccttttgata acaaactaag tatttgccta tcatgaattt caaacgcttt atccgttagc
      721 tcctgagtaa gttgtaataa atagatggtt aatatcgaat aacgtttatt ttcttgaaag
      781 tcacggaatg catacggctc gtatcttgag cctaagcgag acagctgcaa caggcggtta
      841 cggtgcaaat gactaatttg cactgtttct aaatccattc ctcgtatgta ttcgagtcgt
      901 tctattattt ttagaaaagt ttcgggtgaa ggatgacccg gtggctcttt taaccaaccc
      961 aatatcgttt tattggattc ggatggatgc tgcgaggtaa taatcccttc aagcttttct
     1021 ttttgctcat ttgttagaga tttactaacc gtattaaata gcttcttttc agccattgcc
     1081 cttgcttccc acaccattct ttcaagtgta gtgatagcag gcagtataat tttgtttttt
     1141 cttagaaaat ctatgcattc atgcagtaga tgaatggcat caccattttc caaagctaat
     1201 tgatgaaggt acttaaatgt cattcgatat tcactcaggg taaaagttac aaagtcgtat
     1261 tcacttcgaa tttctttcaa atgatcccaa agtgtatttt ccctttgagg ataatgatca
     1321 agcgaggatg gactaacacc aatctgtttc gatatatatt gtatgaccga atctgggatg
     1381 cttttgatat gagtgtatgg ccaaccggga taccgaagaa cagctaattg aacagcaaat
     1441 cctaaacggt tttcttccct ccttcgctta ttaactattt ctaaatcccg tttggaaaaa
     1501 gtgaagtagg tccccagtat ccattcatct tcagggattt gcataaaagc ctgtctctgt
     1561 tccggtgtaa gcaattctct acctctcgca attttcattc agtatcattc catttctgta
     1621 ttttcaattt attagttcaa ttatatatca atagagtgta ctctattgat acaaatgtag
     1681 tagactgata aaatcatagt taagagcgtc tcataagact tgtctcaaaa atgaggtgat
     1741 attttgcgga aaatcggtta tattcgtgtc agttcgacta accagaatcc ttcaagacaa
     1801 tttcagcagt tgaacgagat cggaatggat attatatatg aagagaaagt ttcaggagca
     1861 acaaaggatc gcgagcaact tcaaaaagtg ttagacgatt tacaggaaga tgacatcatt
     1921 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
     1981 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
     2041 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
     2101 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
     2161 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
     2221 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
     2281 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
     2341 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
     2401 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
     2461 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
     2521 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
     2581 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
     2641 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
     2701 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
     2761 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
     2821 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     2881 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     2941 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     3001 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     3061 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     3121 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     3181 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     3241 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     3301 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     3361 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     3421 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     3481 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     3541 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     3601 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     3661 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     3721 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     3781 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     3841 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     3901 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     3961 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     4021 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     4081 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     4141 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     4201 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     4261 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     4321 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     4381 tctttgtcaa atgatgttgg taaagagaaa tcttagaata
//
LOCUS       DACEZN010000184         4301 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEZN010000184 DACEZN010000000
VERSION     DACEZN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.184"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            186..725
                     /locus_tag="IXH60_002553"
     CDS             186..725
                     /locus_tag="IXH60_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1991437.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            718..2991
                     /locus_tag="IXH60_002554"
     CDS             718..2991
                     /locus_tag="IXH60_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1991438.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            3029..4075
                     /gene="holA"
                     /locus_tag="IXH60_002555"
     CDS             3029..4075
                     /gene="holA"
                     /locus_tag="IXH60_002555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1991439.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(4164..>4301)
                     /locus_tag="IXH60_002556"
     CDS             complement(4164..>4301)
                     /locus_tag="IXH60_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1991440.1"
                     /translation="ASGADNVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIA
                     K"
BASE COUNT         1348 a          686 c          894 g         1373 t
ORIGIN      
        1 tacggaattt cttgaacaaa tggattcgaa gaatttttat aatgggctaa tggatgacgg
       61 acttcatttc ggcagtgaag ggtaggatct tttgtcagac aggatgattc gttatattta
      121 aagaatattt tgtttaagca atgagtactt tttaatcaac ttgaaaaaac ggaggaatat
      181 aaaacatgtt agaaaaatct tcaaaagcat gcgatacgaa taaagaaaaa gttactgata
      241 agtcacataa tgaacgcatt ccttgggacc agtactttat ggctcaggca gtactgttat
      301 ctttacgtag tacgtgtaca cgtttagaag taggagcgac actcgttcga gaaaaaagga
      361 tcattgctgg cggctataat ggagcagtca gtggagatgt ccactgcata gatgaagggt
      421 gttacgtcgt ggatggacac tgcttgcgaa cgatccatgc agagatgaat gcactcctac
      481 agtgtgcaaa acttggtatc ccgacggaag gttcagaaat ctatgtcaca cattttccat
      541 gtttggcctg tacaaaggct ttgttgcaag ctggtgtgaa agggatcaat tatctccatg
      601 actatcgaaa tgatccttat gcccaagcgt tgatcgaaca attaggtgta tctgtacacc
      661 aagttgcttt agactcacag catttttcaa aacttcaaaa tgaattaaca ggaaaacatg
      721 tctagcgttt tagaaaggca tgttcgaaat cggttgattt ttccggcaat tacgtcgatg
      781 attatctgtt tgttgtatta cactgacaat tggggttatc gtttgcttct tgccggaatt
      841 tttttatggt tttcgctaaa agaaggacgt tttatcgcgt acttgtgtat tttgtgtagc
      901 ataacggtat ccatttctta taggctacaa gagtattcat ttttagagga aaaaacaaca
      961 tctgcgcaaa ttactgtatt tacagatacg attcgtgcaa atggtaattt tttgacaatg
     1021 gaaggaaggt tatcagagaa taaacaaaag ctccagcttt cttatcaagt aaacacagaa
     1081 gcagaattga aaacatggct taacttccga ggaaagggaa tagaattgac tgctgaaggc
     1141 gagttcatct ctccagcaaa aaagcggaat ctttataatt ttgaccaaga agcttacttc
     1201 aggaaccata gaatttctgg acggttccaa attcaaaaga ttgaacagat caccatgcaa
     1261 aatcactggc gaaactggct tcagagaaaa agaggatctt ttatctcctt tgtccaatct
     1321 gaatttccgc aaaaaacaag tgtatatatt aattctttat tatggggcta taaagatgca
     1381 ggattcagag aaccagaaga aatttttagg gagagtggtt tgctgcatct gttcagtcta
     1441 tcgggtctac acatccaatt ttatttaggg tggctgtatt atttatttcg acgtatcgga
     1501 tggccactac acgtcagtat tattccgttg agtctactaa ctatctgtta tgtttctatt
     1561 gcaggaagtt ctatttctgt tttgcgtgct agtttgctgt ttttattgcg attgctagtc
     1621 aaattgctga ataagcatta ttccaccatg gacttatttg cagttgtctt ttggcttctt
     1681 ttactatttg atcctcgatt tctttttcat gcgggtggac agcttagttt tttcatgagt
     1741 tttttgttta tcctgatttc ttttgatgga gattggttga aaaaaacggc ctctcacgtc
     1801 ctgtttacta tgatcacgat gccgttgatt gtatggcatt tttatgagtg gcctatttta
     1861 ggtagtcttt ttacattact gattgcaccg gtattcaagt ttctttttct tcctggtgtg
     1921 ttatttctca cactcttcaa tcgctggctg ccggaaactt tacttttgtt gatagaagag
     1981 ggattaatct tatttgagcg aataatcgat ttttcttcag ggtcgtctct cattatagga
     2041 cgtttgcccc ttgtgctatc tgggatgtat attttcggat tactgatttg tatggatagg
     2101 ttgaaagaac aacctgtgaa acacttgttt tatgttggtc ttttttcttt tatttttttg
     2161 actttgccat ttttacgctt ttcttcaacg atcgctttta tcgatgtcgg tcaaggagac
     2221 agtatctttt tgcaaagtcc ttttcgaaaa gaaacgattt tgatcgatac tggaggtcag
     2281 ttacactttg aaagagaaaa gtgggcgcaa ggtatgatac gtcctcctgc tgaaagaaat
     2341 atcgttccat atctaaaagg tcaggggata agtgtaatcg ataaactcat cttgacacac
     2401 gacgatacgg atcatgtggg agaattaccc acgttatctc agcattttac tatcaaaaaa
     2461 atttatatcg gttggggcgc tggacgaaat gagccattac aaaggcattt atctgaagct
     2521 caaaaaaatg gtactgagat cattgaaatc aaacatgggg acaggatcag cggttattat
     2581 gatctttatg tattgactcc ttttgaaaag ggagagggaa gaaatgaaga ttcgattggt
     2641 ctatggatgg aatataacaa taggcgcttt ttatttttag gagatttgaa tcaagcaatg
     2701 gaaaaacaat tgcttttgat ttatcctaat ctgaaagccg atgtagtcaa attaggtcat
     2761 cacggtagcc gtacgtcaag taacacagat tttctttccc atattgaagc aaaacatggg
     2821 attatatcct gcggcgtaaa taatcgttat ggtcacccgc acagcgaagt attggagaca
     2881 ctagaagaaa atcagataaa aacatggcga acggatcaac aaggaatgat cacctatcgc
     2941 tggcatccag tttttcatcc tcatgggcta gtggaaacaa tgatagacta gtaattctca
     3001 tctgctcatg gtaggataat ttttgaaagt ggtgagtaat ttgagtatac aagcagagtt
     3061 acaaaaaata cgtaaagaaa aacttgcatc ttgttacttg attttaggaa cagaaaagtt
     3121 tctccaagat caagttcgga cagaaattgt caaaaaaatc caattagagg gcgaagatga
     3181 cttaaatttt ttaagttttg acatggaaaa tgcaagttta gatgaggtag tagctgaagc
     3241 agagacgttg ccgtttttcg gagatcaacg gttagtattc gtagagaatc cttatttttt
     3301 aacaggtgaa aaagtaaaca atggaatcga acaaaatact gatttattga cggcttattt
     3361 gaaagaacca ttagaatcga ctgtattagt attctttgcg ccatatgaaa agttggatga
     3421 acggaaaaaa gtaacgaaac aattgaaaaa aacagcaatt atcatcaatg tccaacagtt
     3481 gaatgaaaaa gaagtacgtc aatatctgat gaatacctta gagaattcct caatcaaaat
     3541 ggaacgaagt gcaattgatt tattcttgag gttgaccgac ttagatttat ccaaaatgat
     3601 gagagagtta cagaaattgg tcctttatgg acaaaatcaa cagcaaatca cggtaagaga
     3661 agtagaagaa ttagttccta aaacgttaga acataatatt tttgacatga cccaatatat
     3721 tttaaaaggg aagacggaac aagctttgcg tttatacgaa gatttagtta tgcaagggga
     3781 agaaacaatc aaaattaatg cgattttgct ttctcaatta cgtttatttt tacagacaaa
     3841 atttcttgta aagattggtt atcagcaggc aaatattgct gaaacattga aaatccatcc
     3901 atatcgtgta aaacttgcca tgcaggaagt aaaaaaattc gatgaacatt tgctggtaca
     3961 gttgtttgat caattagttg agatggatta tcaaatcaaa acgggacaaa tcgaaaaaga
     4021 attaagtttt caactattcg ttttgcgcac agggcagttg ttaagaaatc gataagaaga
     4081 aagacgaggg caaagcatgc gggaaggtcg tttgtttttg tccttttttt gacaaataaa
     4141 aaaatcggcc gaagccgatt cttttattta gcgatttttt tgcttaaacg agatttgtcg
     4201 cggttagctt tgtttttgtg gattagacct tttgattcag ccatatcaat agctttaaca
     4261 gcttctttat ataacgcatc tacgttgtca gcacctgaag c
//
LOCUS       DACEZN010000185         4249 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEZN010000185 DACEZN010000000
VERSION     DACEZN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.185"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IXH60_002557"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IXH60_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1991441.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="IXH60_002558"
     CDS             complement(2066..3064)
                     /locus_tag="IXH60_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1991442.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="IXH60_002559"
     CDS             complement(3290..3943)
                     /locus_tag="IXH60_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1991443.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         1249 a          897 c          658 g         1445 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagt
//
LOCUS       DACEZN010000186         4177 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEZN010000186 DACEZN010000000
VERSION     DACEZN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.186"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..492
                     /locus_tag="IXH60_002560"
     CDS             <1..492
                     /locus_tag="IXH60_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1991444.1"
                     /translation="DAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETIGKPVI
                     FVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDCHSIEL
                     CGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDKRFLTF
                     LEQ"
     gene            660..2573
                     /locus_tag="IXH60_002561"
     CDS             660..2573
                     /locus_tag="IXH60_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1991445.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            2578..3579
                     /locus_tag="IXH60_002562"
     CDS             2578..3579
                     /locus_tag="IXH60_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1991446.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3781..3987)
                     /locus_tag="IXH60_002563"
     CDS             complement(3781..3987)
                     /locus_tag="IXH60_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1991447.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1538 a          645 c          864 g         1130 t
ORIGIN      
        1 gacgcaatag tagtgatcga tttacaaaaa ggggtatgtt ttgattcttc gccgatacac
       61 gaattagaaa cagtgacagc attagtgaat aaaagaatca aaacatacga aacgattggg
      121 aaaccagtta ttttcgttca acacgaagat gctcaattag tgccacaaac aaaaccatgg
      181 gagctattgt cggaattatc tattcctgag cacagttatt ttgtgagaaa aacccatgcg
      241 aattcatttt ataagactaa tttgagtgtt ttattaactg aattggactg tcattcgatc
      301 gaattatgtg gggcacaagc agagtattgt atagatacga ctgtgaagtt tgctcatgga
      361 ttaggatacc agcttatgat gtgtagagga gcaacgacaa cttatgataa tccttttatg
      421 aaagcaaagg aaacaatcgc tttttacgag aaaatttggg ataaacgatt tctgacattt
      481 ttggagcaat aagttgtttc ttcgtttaaa aaaagcagaa tggatactgg tttatccatt
      541 ctgctttatt acgtaaaatg agaaaataat cctgttctat ttttatggga taaaccattt
      601 attttttgta taaaaaatgt ttgcgtttac atataattct cctagaaaca taaaaaggag
      661 tgaatagctt gagaacaaaa caagtattaa agggagcgtt ggtcatctta tttgctgtaa
      721 gtacaagcgt cccgcaatgg acagtatatg gagagagcat ttcttccgaa tcggaatctc
      781 gggaggtaga aaccacagaa actactgatg ctggaaatga aacgaaatcg agtacttcac
      841 aaacagaaac aaatactgaa acaacggttg agacatctgt tactagcaca gatcaagaaa
      901 cgaccgctac cacagataac caacaaacag aagaagcaac agctacaaaa atgtcaaacg
      961 acgaatcctc ttctgctaaa gtaagtgaca aagagaatgg gacagaaaat gatgatatcg
     1021 gtaaaggtga aaatttcaat ggtaaagaat atttgaatta cttcggtaaa cagatgacag
     1081 aaatacttaa tggaccaaca gaagacatca agtcaaaatt aatagagtta ttagtcactg
     1141 agcttgctgt taatgattat ggtgtagata aagagattat caaacatctg attgctactt
     1201 atctattgta cgatatcgct tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta
     1261 cagatgatgg gacaacgcct acagaaaaca caggcttcta tccaaataca aaacaacttc
     1321 atcaaactgt actaaacaaa caagatggca aaatgatgga tgtatatgca tattatgtcg
     1381 atcaaggttc agataaaaca gtcatgatcc atggcggatt tcgagggaat tggaataacg
     1441 ggatcgttac agaagaatac aatgattttt ataaggccgg gtataattta ttatttgttg
     1501 attccagagc gactggtaat agcggaggcg attatgtgac ctatggacaa tatgagtcag
     1561 atgacgtcct ttactggata aatcaagaag ttcgagagcg gccatctcaa aagattttac
     1621 tttatggtgg ttcgatggga gcagctacga tgatgtctgt attagcgaaa gatattcctg
     1681 tcaatgtcaa aggaattatt gaaaattgcg gatttgcatc aattgatgaa caactacgtt
     1741 ttacgtacag ccagacagta gcccccgcac taccggatgc cataaaaaat cagttagaca
     1801 tcattgggga tcaagagcat gaagatttat ttatgggcct tttgaaacaa tactattttg
     1861 atcaggagat gcatttggat acaaaagctg ctctacctac gattggaatg tcagattcat
     1921 tgcctaaatt gattatccat ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa
     1981 aattatatga gttatcagga gggtataaag atcttttatt agtagaagga gcagggcatg
     2041 gaaaggcaca ggaagttgac catgctgcct acacgaaaca tgtaacggac tttttgaaag
     2101 tagtgttcca tgatcagata aacgtaaagt atgtggatga aaacaataaa agtctactca
     2161 aagaccagga cgaaatccag ctttacggag cttatggaga aaattatatg acagagcaaa
     2221 agacattcga aggatacgaa ttagcaaatg ttgaaggacc gactgaaggg atttttaatg
     2281 agacgatacc aactattatt ttctattaca aaaaaatccc agtagctcca ccaaaaaagc
     2341 aagatccgac agagaatgca gacaataagg gaaagaatga acctcgagag gatcaatcaa
     2401 gtgatgatac gcaaccgatt cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa
     2461 aaagaatgtt gccgaaaact ggagaaaaga aacattcgct tctcatttat tcaggtggat
     2521 ttttatctgt tagcacgatt atcacatgga tttggcgaag aaagagaggg taaatgaatg
     2581 aaatggttca agcgaatagt gattgcagta ccagttatca tcgtagttat tttagtcact
     2641 gtatttttga ttaatcaaat cacaccaaag ccgattagct tgcttgttag aaaacaattt
     2701 gacacaagtg aaaaagaaca agtatatgcc cgaccagatg atttccagca aaaaatcgaa
     2761 caaatcgata taaaaaaaga aatatcctat ccttcaaagt acaaaaataa taatatggat
     2821 atctacacac cgaaagtgga aaagaaaaaa ttaccgattc ttttctggat gcatggaggc
     2881 gcatatgtag gaggggataa aaatgattgt cgtgattatt tggaatatct atgttcagac
     2941 acgcagcaaa tcatcgtaaa tatagattat gaacgatcac cggaagcaaa acatcctaca
     3001 ccagttatcc aattgaacga agccattcaa gcagctaaaa aagagataaa agatcaagca
     3061 gattggtcga atatttcgat tggtggaaat tcggcaggtg cacaaattag tggagaatat
     3121 ttgttagcac ttcaaaatga agaaattaaa aaagctgatc atcttgatcc gactttggag
     3181 aacaagcaga ttacgaagtt tatttctttg tctggtctgt tggatccttc tgcacttact
     3241 caagtaagtg ataaaatctc tagctttctt tatgaaaaat gtggatgggc ttactttgat
     3301 aataaggact ttgaacaatc agagaaggtc aaaagtttag ccttggtcag acatgcggat
     3361 cgttggacgc aaaacacatt cttaacggat ggaaatacaa atacgtttac gaaacaaatg
     3421 gaagaagtaa cttctgtatt tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg
     3481 gagaaaaatg ctgttttgaa ccatgaatat cagtttgatt tcagcaataa acaagcgcaa
     3541 gaaacatatc aaaagttagt aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg
     3601 ttgtaatctg tcacttagct ccaagtcaca aggagcaatt tcaataaaca agcttttcat
     3661 ggtttatgaa tattgtaact tgtgactgag gagttagaga tggcaagggg caccttttat
     3721 tcgaaatttg ggaatatcac acgattattg tcacgatctc ttttacgtaa attttataag
     3781 tcagatttgt tgttttgatc gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag
     3841 aagggacaaa aagccgagtg gtaagcaaaa gaatgctggt tccactaata ttccgttgtt
     3901 attgatataa ggctgggaac aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa
     3961 aagcaaagaa atatggatag ctttcataag cagcacctcc ttttttataa ttgtatgata
     4021 aatagattaa gaatctataa atgtgacaag tttgttagga tgtgaataaa atgatcatct
     4081 agcgaacttg aaacaaagat ggctaatgat aagcaattaa tgattcagca atcagatagg
     4141 tacaaagttt accatcatac gtctagacca ataatag
//
LOCUS       DACEZN010000187         4174 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEZN010000187 DACEZN010000000
VERSION     DACEZN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.187"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            612..1424
                     /locus_tag="IXH60_002564"
     CDS             612..1424
                     /locus_tag="IXH60_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991448.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            3059..3613
                     /locus_tag="IXH60_002565"
     CDS             3059..3613
                     /locus_tag="IXH60_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1991449.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            3867..>4174
                     /locus_tag="IXH60_002566"
     CDS             3867..>4174
                     /locus_tag="IXH60_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991450.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT         1542 a          516 c          660 g         1456 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt
     1921 ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt tgtttgtaat
     1981 tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc agacattcta
     2041 tgctcatttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     2101 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     2161 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     2221 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     2281 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     2341 aagaactagt agaagaagga ataatggctt gataataaag tactacctat taaaggtagt
     2401 actttattta atttagtata aattaacata ctactatatt ttaagtttca tattatataa
     2461 attaataata tactaccatt ttttggtagt ataatttaaa ggtaataagt tattctttta
     2521 ttactatgtt tgggttttat agcaaggaaa gtttaataag agtttggacc tagcaaacaa
     2581 tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat ctacacagac
     2641 aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat taatgaaaag
     2701 cttggtagca cggttattca tatgctaatt tatttcactt tatagtaagg ggagttctta
     2761 gtatattgtc caaatttaaa tttcatataa tttaattatg taaattagct attctaagaa
     2821 cttctacgga taactgcccg tctaaaattt aattaagagc ttagaatctt catataagta
     2881 aggttaattt ttttgtacaa aatagtcgga atattctgag ttactgaaat gatataaaat
     2941 cataaactaa accccttgat tttcttcact tttaacagag tatatatata agttcatgat
     3001 tatggtaaaa tatatatata atcatgaact cagaatattt ttaagaaggg aaacaatagt
     3061 gagctataat gtacaaccgt taaggacaaa tgaagagata caagattttc agttttggct
     3121 aagaagaaca agtaatccag aaagagatag ttttttattt ttgtttggaa tcaataatgg
     3181 gttacgtatg tcagatatta taggattaaa agttggagac atacgaggta aaagtaagcc
     3241 tattattgtt gaacataaaa caggtaagcg aaaaccgatt tttatagata atttgagaga
     3301 agagatttta ttatatactg aaggtaaaga agaaaatgat tggttatttc caagtcggca
     3361 acaaggtcga catataacaa gggatcgtgt ctatcagata tatgcagata tagcagaaaa
     3421 attaggaaga gatgatatag gaacacatac attaagaaaa acttttgggt atcactacta
     3481 caagaaaact agagacattg ctaccttaat gtttattttt aatcatagct cgcaagcaat
     3541 tacaaaaagg tacattggta taactgaaga tgaaataggg gcatcgttac gtggttttaa
     3601 attgggaggc taattgagat attaggatat attttttgtg tataatgaaa acttagaagg
     3661 tgagattaat gaagataact gaattttcgg aagtatttaa aacgtttagt aacatttaca
     3721 atagaatacc agagatagaa caaaatgttg tagttgataa tgattattat atagagaatg
     3781 attttaataa accaagaaaa tatgacaacc tatacaattt tatacacaat agaacaacaa
     3841 taaataacaa cagtaacact cgagttttga ttaattcagc atattttcat attaaatatt
     3901 taaagaaata tgagtatgaa gaagttgtat tagattatgg gctaaataga cagtcatttt
     3961 taatttatgc aataaataac ggaaatttgc caagcaataa aaagaacata attattgaaa
     4021 catatgccta tctggagggg ggatctaaaa aactaattaa gtcatgtaat atagagtatc
     4081 aattatcagt aggagaaatt aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa
     4141 aaatttttgc cagcattcat gattatatag gttt
//
LOCUS       DACEZN010000188         4124 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEZN010000188 DACEZN010000000
VERSION     DACEZN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.188"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(152..700)
                     /locus_tag="IXH60_002567"
     CDS             complement(152..700)
                     /locus_tag="IXH60_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991451.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(701..1558)
                     /locus_tag="IXH60_002568"
     CDS             complement(701..1558)
                     /locus_tag="IXH60_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1991452.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1844..2131)
                     /locus_tag="IXH60_002569"
     CDS             complement(1844..2131)
                     /locus_tag="IXH60_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1991453.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2121..2450)
                     /locus_tag="IXH60_002570"
     CDS             complement(2121..2450)
                     /locus_tag="IXH60_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1991454.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2527..2799)
                     /locus_tag="IXH60_002571"
     CDS             complement(2527..2799)
                     /locus_tag="IXH60_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1991455.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(2799..3062)
                     /locus_tag="IXH60_002572"
     CDS             complement(2799..3062)
                     /locus_tag="IXH60_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1991456.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3168..3584)
                     /locus_tag="IXH60_002573"
     CDS             complement(3168..3584)
                     /locus_tag="IXH60_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991457.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(3590..>4124)
                     /locus_tag="IXH60_002574"
     CDS             complement(3590..>4124)
                     /locus_tag="IXH60_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1991458.1"
                     /translation="WVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAI
                     DADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLY
                     TKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTL
                     FSFSVSTEIKVLMGITA"
BASE COUNT         1181 a          823 c          562 g         1558 t
ORIGIN      
        1 tccattactt ttcaagattt aatttctttg tattcactga ataatttcca cgttttttcg
       61 ttaatgttac ttcttattaa ttttattgta cttctcatgc aataggatca tatatccttc
      121 taaatctttt ttatgctata aaaagggggt tctatctaac tttattattc attccccacc
      181 ctttgcgcag tgagtcttcg gtatcttgtt ttatctgact ggtagttctt attatttctc
      241 cacggtcatc taattctgga acaattggat atttttctct gaacaagtct agttctttca
      301 ccacgtcatt aaacaaatct tgcttatctt cttgcgtaag attctgttta atatactcat
      361 ctaaagcttt tctaagcaat tcatctcttg tcatattagt ctcatacttt tctactaaaa
      421 ctctggaagt cgcatttaac aaagtcgaca gttttaaagg atgcttggtg gaataaacct
      481 tatcaaactt tctgattggt gcgccaactt tttttgttct tcctctactc tgtctttcaa
      541 cattggagga agtttttatt tcttgaactt ggtgcacttc taattgctta ttttcagatg
      601 gtgtaattgc ttgctgcttt ttctttgctt ctgcttcttt ttttattttg gcgagtcctt
      661 tgttgttccc aaaaatatca agtcctttga tgtcagccaa ttactccacc tccgcatacc
      721 aattctcgtg ttcttccaat tctttcaaaa tactgataaa taattcgtgg cttttgcgat
      781 cccattgttt taagagacct ttctttgtct tctccatcgt aatacctgat ccgtcgtatc
      841 gttttaagcg cgcatttttg agtacaacat ttttaatcaa atgttcccca taaatctctt
      901 gggcttgttt atacatttcc tgatctactg cgtcaccgct ttccaacatg aaaggaataa
      961 caccagcaac atataaattt gtatcaaatt ctttcacaaa aaagtccata tactcaagat
     1021 attgcatcgc atttctcatc gattttactt gggtctgtaa gacgacgatt gcccagtccg
     1081 caatgtacat cgcggattta ctatatacag aaatagaagg tgggacatct aaatagatga
     1141 cgtcaaactc atcaaataat ggttctagct tttctttcat atatttgaat tgggataatg
     1201 aatcattata ttggtctttg atccaatcat catacatttc aaaggacata tctgctggaa
     1261 gtatatagag gttattgatt actttataga cagcctcttt aggtggaaga ttgtttatca
     1321 acacgtcagt cacagtattt tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt
     1381 tagtgaagtt catatgcgtt aagttcatca catcagaagt atttccttgc atatccaagt
     1441 caattatcaa ggttcttttt ccttcattaa ctgctgattc aaaccctaac atttgcgcgc
     1501 acgtactttt acctacgcca cctttaaaat taccaattac atatttcttc gctaccacgt
     1561 tcctacctct tttcttttag caatattcat ttgatactat tataacatat ttatttttag
     1621 ttactaccaa aaaaaagata tatatgtact accttataat taataagtaa aaggtagtac
     1681 ataaataagg aaaagttgaa atatattgat ttatagcttt cctcaaagta atgacgttat
     1741 ttaatttatc gagaagcaga gaatactcgc gactttagtc atgtagacga atcagatttt
     1801 cgataaaaaa gaagccgtcc ttcttaagaa cgacaacaaa tcactagatt aattttttca
     1861 actgttcata aaagttttca tgcgttccac ataacaatac tggaataacc ttgttttcta
     1921 taatctcgta agctactcta tatgttgtgc ccgcatattt gaacccacta gaccaaatac
     1981 ctgataaatc cccagttttt tgttcaccct tatatggatc aatcgcaatt tcatcataaa
     2041 ttaaattcaa aaatttttct ttcaatggct tctcttttag cttttttaaa tacttaatga
     2101 catgttttga aggaacgatt tcataagcca atttctgttg ccaactcctc tttagtcatt
     2161 actttagaat tagtaagtgt atcttcagca atatctctaa aggaagaatg cattttgttt
     2221 tttctattct taaattctac aagaatctca tctttatcgt agccttctgc aattatatca
     2281 gacaaaatat cttcgctaaa atcataaaat tctttttgtg gttcaacaaa ttcataaaga
     2341 ataccattct ctaccaactt tgcttcaact tcgactccat taggaacatt aaaatcttta
     2401 ggtactgtca acatgattga attgccttgt tttcttgttt ttgtaatcat ttaaatccct
     2461 catttctaac agtatgtaat tacttagtaa atacattata ccacgagata atgtatttac
     2521 aagtaattac tgtgactcgt aatgcgtcct tgctgaataa atttcaacca cgtgttcatc
     2581 ttcatctacc ttataaacaa cccggtgttg tctattcaat cttctagaat acctaccttc
     2641 atatgtaggt tttaacttct caaatgattg ggtagggaga tagggatctt cctttaaagt
     2701 ctgaataacc ttctcaaatt gaggtttcaa attagactgt ttgattttct ttaaatcagc
     2761 tttagccgag tttttaatca tcacagaata gttattcatt ataagttatc ccaatcaatt
     2821 tcatcaacat tagtaaatcc actatcatct ttttctcgct tacgaacaac acccaaagtg
     2881 cctatttgct caagtaatag cgtttctttg attgctttca aatcatccaa accaataagc
     2941 accgcattat tttcactgcg ctcactaatg atttcaattt ccgtatgatt ctcatttaca
     3001 tctttcaaca acttataaaa atttttccgt gctgagctag gatttaagac tgtttttgcc
     3061 atatctactc cccctctaat ttatatctcc attctaacgt acttaattaa gtacgtaaat
     3121 tcgattgttt tgtttaaatt ggagtaccaa aacagcattg taaagtgtta tccgtttcct
     3181 ttatttttat taacaagttt tatcacaggc ttcatatatt tgattataac ttcatcttct
     3241 acgtagatat ctgttatcat tacgacatct gtactatttc gattgagtac tttcacttga
     3301 tctccagcat tgattttccc tctaaatctt ttaggaatga accaactata catatgatta
     3361 gcatcaccaa aatgtgcttt tatcgcattc tcaggatata acttattcaa tagttcattc
     3421 tttttaattt tcaaaacttc aaatctatac ttatcatcat ctgctaatct aaataaatct
     3481 tctaaacgca tgttttctaa ctgtaagttt tgaaaatctt ccggtataaa ttttcggttc
     3541 tgttgcgaag tttgaaaatc aaacgcgccc tctctgaact ccaaatatct taggctgtta
     3601 ttcccattaa taccttgatt tcagtagaca ccgaaaagct gaagagcgtt ccatttcttc
     3661 ggttcttttt atatattcct cgaatggtct ccatgccctt aatcgtggaa gaggctgtac
     3721 ggagactttg ataaaattta ttccgtcgtt taataggtcg atggtcttgt tctattaaat
     3781 tgttaagata cttcacagtt cggtgctctg tcttagtata taaacccaca ctctgtaact
     3841 ttctaaaggc ggagccaaga gaaggtgctt tatcggtcac aattgctttc ggctcaccaa
     3901 actgtttatg gagtcgtttt aagaaagcat aggctgcttg cgtatcccgt ttctttcgta
     3961 accagatatc taaggttaag ccgtccgcat caattgcacg ataaagataa tgccaacgtc
     4021 ccttaatttt gatataggtt tcgtccattt tccatgaata gaaggattgt ctatttttct
     4081 tcttccaaag ataatagagg actttgctgt actcttgcac ccaa
//
LOCUS       DACEZN010000189         4034 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEZN010000189 DACEZN010000000
VERSION     DACEZN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.189"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(176..1462)
                     /locus_tag="IXH60_002575"
     CDS             complement(176..1462)
                     /locus_tag="IXH60_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1991459.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IXH60_002576"
     CDS             complement(1455..2723)
                     /locus_tag="IXH60_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1991460.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IXH60_002577"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IXH60_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1991461.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IXH60_002578"
     CDS             complement(3495..3941)
                     /locus_tag="IXH60_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991462.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1138 a          821 c          711 g         1364 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttt
//
LOCUS       DACEZN010000190         4027 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEZN010000190 DACEZN010000000
VERSION     DACEZN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.190"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            474..725
                     /locus_tag="IXH60_002579"
     CDS             474..725
                     /locus_tag="IXH60_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991463.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IXH60_002580"
     CDS             759..1037
                     /locus_tag="IXH60_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991464.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKK"
     gene            1634..1891
                     /locus_tag="IXH60_002581"
     CDS             1634..1891
                     /locus_tag="IXH60_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991465.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="IXH60_002582"
     CDS             2004..2615
                     /locus_tag="IXH60_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991466.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="IXH60_002583"
     CDS             2794..3351
                     /locus_tag="IXH60_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991467.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
BASE COUNT         1439 a          538 c          827 g         1223 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaaataatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttactt
//
LOCUS       DACEZN010000191         4012 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEZN010000191 DACEZN010000000
VERSION     DACEZN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.191"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            179..775
                     /locus_tag="IXH60_002584"
     CDS             179..775
                     /locus_tag="IXH60_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1991468.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IXH60_002585"
     CDS             899..2698
                     /locus_tag="IXH60_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1991469.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IXH60_002586"
     CDS             complement(2976..3188)
                     /locus_tag="IXH60_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991470.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1178 a          686 c          710 g         1438 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata tt
//
LOCUS       DACEZN010000192         3965 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEZN010000192 DACEZN010000000
VERSION     DACEZN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.192"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..755
                     /locus_tag="IXH60_002587"
     CDS             <1..755
                     /locus_tag="IXH60_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1991471.1"
                     /translation="NDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISEVTLDETDDF
                     TYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDTILV
                     PLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKY
                     EITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLA
                     HPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            822..1316
                     /locus_tag="IXH60_002588"
     CDS             822..1316
                     /locus_tag="IXH60_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1991472.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1372..2010
                     /locus_tag="IXH60_002589"
     CDS             1372..2010
                     /locus_tag="IXH60_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991473.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2082..2477
                     /locus_tag="IXH60_002590"
     CDS             2082..2477
                     /locus_tag="IXH60_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991474.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2461..>3965
                     /locus_tag="IXH60_002591"
     CDS             2461..>3965
                     /locus_tag="IXH60_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1991475.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKA"
BASE COUNT         1413 a          596 c          753 g         1203 t
ORIGIN      
        1 caaatgatca atttaaatct cttttaatta aaacattaga aagaaagagc tcttcaaatt
       61 attatgaagg aggtaacgag attgtttcta aaatgaaaat atcagaggta actttagatg
      121 agactgacga ttttacttat tataaaggat ataaaaaggg ctggaatacg ctttgcacat
      181 atcttaaaat aagagttcct tttgatgatt tagacttttt tgaaagtcat aaagatttga
      241 ttacccagac agcaagctcc atatatgata aacaaggtga taatgtatta gtggatacta
      301 ttttagtacc acttccagag aattacgaag taattaactt tagtcagttg aaaattagtg
      361 atgtggtttc acaagctatt gaagacgctg agtcttttat gagtaatggt gaatatcaac
      421 gtgcctttga cagagttcac actgcttttc atggatattt aattgaaata ctaaaaaaat
      481 atgaaattac agttcctaga gacgaaaatc tatctaaatt atatagtagg atacaacaat
      541 taattgaaaa agaaattcaa cctactgaac ttgctgatat agtgaagaca acgataaggt
      601 catcaaatgg aatgattagc tctcttaatg aagcaagaaa taggcattca cttgcacacc
      661 ctaatacaaa tattattgga aagagagaag caaaactaat aataggtatt tcaagtacag
      721 ttacagatta tattagcgga taccttgata agtagcgtca ttaactgatt gctacttttt
      781 ttatgactat ttttataaaa aggatgtgat aagaattgga aatgaaagtc tatgtggcaa
      841 atttaggacg gtataatgaa ggtgaattag tcggagcatg gtttactcca ccaattgatg
      901 aggaagaaat ggcagaaaga atcggtttaa atgaggacta cgaagaatat gcaatacatg
      961 attttgagct tccttttgat gtggacgaat acacgccgat tagtgaaatc aaccgtctat
     1021 gtgaagccat tcaagaaatc gaaggtacac ctatttataa tgaactcaaa gaaattcaag
     1081 gcatgtggtt tagtagcttg gaagaattac tggaaaacaa agaggatatt cattgttata
     1141 gtgattgtga ttcgatggaa gatgtcgccc gttattacgt ggaagaaaca ggacagcttg
     1201 gtgaagtccc ttccaattta caaaattata ttgactacca atctttggga cgtgatatgg
     1261 aaatagaggg taattaccta gttacctctc atggggtatt tgaatattgt cagtaagttt
     1321 aacaatccgt gttataatta aattaataaa tagcacggag aggtataaat tatggtaaat
     1381 gtatttgaaa atcttaaact attaaaacta gacatgaaag ataaaggctg ggtgatagat
     1441 tccttttact ttcgttacaa acagcaaaat tatattgtac ttgttaagct atttgaaaaa
     1501 gaggaaaaag ttccagaata tgctctgtta aaactagaat ttttaaagga aaacgatttt
     1561 tcagatatgc tagctgtcta tgctaattcg gtaaaattat atacagacac gaaaacaata
     1621 agagaatatt ttggtatcga atacagtagt aacttgggag atgttttatt tcaatttagc
     1681 caaactcttg ctcgatttat tccgacagaa gttagtgaga agaaaaatga agaccaaaaa
     1741 gaagccatgt gtttctcatt aagtcaaagt gattcagaag atcccagaaa aaaatattgt
     1801 ttttcagtta gaagaaatcc tttaaaggga aatggtgagc tgggaaaacg aagtcctttt
     1861 aatgacaata aaacgagatt gtatagaccc agcttatatg aacgattagg gtcagatacc
     1921 aatttaagtt tcagatattc aatgaatcca gatgatgaag aaactgatga aggtattatt
     1981 gcaaaatgga caaaaaataa aattgaatag ttaaagcatt gaaagagcaa tcacctattt
     2041 tgattgttct ttttctattt ttacggaaag gacgtgaaga catgaaaaaa ataagaagtt
     2101 acaccagtat ttggtcagta gaaaaagtga tttatgcgat taatgattta caactccctt
     2161 tcccaattac ctttacgcaa atgacatggt ttatcgtttc tttgtttgtc gtcatactct
     2221 ttggcagttt accaccactt tctctgattg acggagcact actcaaatac ttagggattc
     2281 ccattgttct cactgggttc atgtgccaaa agacctttga cggaaagaaa ccctatggat
     2341 ttctaaagtc tatcattacc tatgctctac gccctaaagt aacctacgct ggcaaacaaa
     2401 tacgattaaa aaaagaaaca atgaatgaat cgattacgat tgtaaggagt gaacaactag
     2461 atggcatatc cgattaaata tatggaagat aatctcgtat ttaatcatga tggcgaagta
     2521 ttcgcttatt atgaattaat tccctataat tacagttttt taagttccga tgaaaaaatt
     2581 caagttcatg ataatttctg tcaattgatt gcccaaaatc gtgatggcaa gattcatgct
     2641 ctgcaactag caaccgaatc aagtattcga gccacacagg aacaatcgaa aaaagaaatc
     2701 actggacgat taaaagagat agcttacgaa aaaatagatg aacagaccga agcgttaatt
     2761 tctatgattg gtgacaatca agtagactat cgctttttta ttggttttaa actctacctc
     2821 aatgaacagg aagtatccgt aaaaaatgtt gggaaagaaa ttcaaaaagc atttgcggaa
     2881 ttctttcatg gtgtgaatca caaattaatg ggcgattttg tttctatgcc tatggcggaa
     2941 atcgaacgct tttctaagat ggaacagttg ttagaaagta aattatccag acgctttcag
     3001 attcgtaaat tggagaaaga tgattttggt tatattattg aacatcttta cgggcaaaca
     3061 ggtgtccctt ttgatgacta cgactaccat ttaccgaaac gatacgaaga taaaaaagtg
     3121 ttggtaaaga aatacgatat tctcaaaccc actcgctgtt taatcgaaga aaatcaacgc
     3181 tatttacgga ttgaacagga agataccact acttatgtgg cgtattttac aattaatagt
     3241 attgttggtg aattggattt ccctaatagt gaaatttttt attaccagca acagcagttt
     3301 actttccccg ttgataccag tatgaatgtt gaaattgtcg ccaataaaaa agccttatct
     3361 accgttcgca ataaaaagaa agaactgaaa gatttagata gccacgcatg ggaaagcgac
     3421 aacgaaacca gtactaatgt ggtggacgct ttagaatctg tcgatgaatt agaaagtaat
     3481 ttagaccaaa ccaaagaatc tatgtacaaa ctaagctatg tggtacgggt atcagcaaag
     3541 aatttggacg agctgaaaca acgttgtgat gaagtaaaag atttttatga tgatctgtct
     3601 atcaaattag tacgaccttt tggcgatatg ctaggcttgc ataatgaatt tattccctca
     3661 agcaagcgat atatcaatga ctatattcaa tatgtcacgt cggatttttt ggctggtctt
     3721 ggtttcggtg ctacacaaat gattggtgaa aattctggta tttacgtcgg ttacaatctc
     3781 gatacaggca gaaatattta tctccaacct agccttgcaa gtcaaggaat taaaggctct
     3841 gtaacaaatg cattagcgtc cgcttttatt ggttcattag gtggcggaaa atcattctct
     3901 aacaatatgc tggtctatta ttcagtactc tttggcggac aggcaaccat tgttgaccca
     3961 aaagc
//
LOCUS       DACEZN010000193         3957 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEZN010000193 DACEZN010000000
VERSION     DACEZN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.193"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..864
                     /locus_tag="IXH60_002592"
     CDS             <1..864
                     /locus_tag="IXH60_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase FAD-containing subunit"
                     /protein_id="HAQ1991476.1"
                     /translation="AKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIRSGYYMFQYL
                     MHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGNGEVFKPSTW
                     FGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAATQAAHFGLS
                     YAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALALIAGLFTWL
                     CSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPWLQRTLGNWW
                     YGRPSSQYGKK"
     gene            1321..1737
                     /locus_tag="IXH60_002593"
     CDS             1321..1737
                     /locus_tag="IXH60_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1991477.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            1751..2317
                     /locus_tag="IXH60_002594"
     CDS             1751..2317
                     /locus_tag="IXH60_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1991478.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            2340..3326
                     /locus_tag="IXH60_002595"
     CDS             2340..3326
                     /locus_tag="IXH60_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1991479.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(3481..>3957)
                     /gene="scm"
                     /locus_tag="IXH60_002596"
     CDS             complement(3481..>3957)
                     /gene="scm"
                     /locus_tag="IXH60_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1991480.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1165 a          732 c          860 g         1200 t
ORIGIN      
        1 gccaaatggg gggtagctaa cctatttgat aagatccatt taagcggatt tttagctatc
       61 gtaatgaaac atatcgtgaa tttgaaatac ttctttgata ttcgttctgg ttattatatg
      121 ttccaatatt taatgcatga gtttttccat atcaaagatg atcgcaacgt gactcgagga
      181 cattcttctc gttacggcaa tgtcttatgg agtgtgccac ttcgtatttt ctatggttta
      241 gtttggttcg ttgaagcttc caaaaaaatc attgggaatg gtgaagtgtt caaaccaagt
      301 acttggtttg gtgaaggatc atggttcact ggaaacagtg tgttcccttg gacatgggaa
      361 tacaatgtaa cagatgttgc cagtggtgct tctgctgctg gtgatgcaac cacagctgcc
      421 agcggagcag gggcaagtgc cggagaagca gcgactcaag ctgcacattt tggtttgagt
      481 tatgcatacg gtgaagaacc aatggctgtg attgagaagt caccagactg gttcacaaag
      541 atcatgcaag tgatcatgcc aaacaatgat gtagcaatgt tcttccaaaa attcatggta
      601 ttctttgaaa ttgccttggc attagcattg attgctggat tattcacatg gctatgcagt
      661 gcgacaacga ttgtattggt gatcacgttc tgcttatcag gcatgtttta ctgggtaaac
      721 atttggttca tttttgttgc tttcgcattg atgaacggtt ccggacgtgc gattgggttg
      781 gatcgctggg taattccatg gctacaacgt acactaggta attggtggta cggtagacca
      841 agttcccagt acggcaagaa ataaatagat aaagaacgtt atttgaatgc aagatttcaa
      901 tagaaagggg ctgagacaaa ccgtcacagc ctccttttta tgtaaaatta aaggaataag
      961 atgcccgaga taagcctcca tgagcaagac acaggtgtca caacctctgt tacaacctct
     1021 tgttataaac ggtgttccag aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa
     1081 cttgaaaagt aattttcgta aattccttct tatttcttga agttgcccac ttctgtcaca
     1141 acctcttgtt ataaacggtg ttccacctgc tgcttctcgg taataagccc aaagaattag
     1201 aaaacatgga aagctgtttt ctattcttcg ttcttattac tcaaagcaag acacaggtgt
     1261 cacaacctct tgtttttatg ctaaaattgg gtgagttaat ctggaaaaag gagaacaaaa
     1321 atgagcctta aagaatttgc tgaaaaaagt ctgctcaaac cttgggatgt ttttattatt
     1381 ttgcttttga ctgttctttc ttttttacca gtcatgattt ttggtcttca gcaaaacacc
     1441 gcaacagcag aaaaagaagc ggtactgcgt gtggatggca cagaaatcaa aacattccct
     1501 ttgatggctg gagaagagag caacacatat acttacaaag atgaacacgg agattataac
     1561 ttgatcgaga tagatggcga tcgagtacgg ataaaagaag cagattgcga tgaccagatc
     1621 tgcgtacgtc gaggctgggc aacgaaaaat ggcgaaacca tcgtctgttt gccacataaa
     1681 ttagtgatcg aagtccgttc aacagatgga ggtgacgaag acagtctgat ttattagacc
     1741 ttcacacacc atgagtaaat tacaaaaaat gatttatatt tcgctacttg ttgcacaagg
     1801 agtgatcatt ggtttattgg aaaatatgat cccttatcct ttttcttttg cgcctggtgc
     1861 aaaactaggc ttagccaatc taatcacgat cgttgccatt tttaccatga aaaaacaaga
     1921 cagcttctta ctactttggc tacgactatt cctgactact ttgctaggcg gaacgatttc
     1981 cacattcctt tatagtatga gtggtgcttt gcttagttat tttggaatgt tattggtcaa
     2041 gcaattagga ccaaaacgtg tcagtatcat cggaattagt gcggccggcg gtttcatgca
     2101 taatgtcgga cagcttttga tggcttcttt cattgcacaa tcatggacag tcatgttgta
     2161 cttgccagtc ctttcagttt tagggatttt atcagggatt gcaatcggga tcgcagcgaa
     2221 ttatctttta cgacatgtgc agacacttca acgattccaa aaagattatg agcagggaac
     2281 caaacaaaaa tggagcccaa tgtttaccaa aaaataatcg tcacacaagg agttttttta
     2341 tgaagctaca tccaatgtgg gaaaactatc ctgctttaag taaagaattg acagaaacac
     2401 taaaacggat ggaagaaact gtccgcctga aaaacaaacc tgtcgaacaa gccatcaaag
     2461 aaacgatcca tgccggcgga aagctgttgc gtcctgccta tcagctattg ttctcacaat
     2521 ttggaccaga gcaagatcga gacaaagcca tcgctttggc agcttctatc gaaatgctcc
     2581 atacggccac actgatccat gatgatatcg ttgatgaagc ggatctgcga cgtggtcagc
     2641 ctaatatccg tagtcagttt ggtaatgtcg tttctgtcta tgcaggagac tatttatttg
     2701 tctgctgttt taaactattg tctgattact ccacttcctt gaaaagtctc caattgaatt
     2761 ctcgcagtat ggaaaaagta ttgaatggcg aattagggca gatggatgat cgctataatt
     2821 atgaactttc tgtaaaagaa tatttggaaa atatttcagg taagacagct gaactgttcc
     2881 aattaagttg ttctgttggc gcttttgaaa gtggaacaag cgagcgcttc gcaaaaaaag
     2941 caggagatat cggactttct atcgggatgg cctttcagat tatcgatgat attttagatt
     3001 atacaaaaga gagccaggaa atcggcaaac ctgtactaga agacatgcgt caaggggtct
     3061 attctttacc gttgatctac tctttgcaaa aaaacaaacc aaagctacta ccttatttag
     3121 agaagaaagc ggcaatgaca gaagaagatg tcgacgccgt acgcaaaatc gttgaacata
     3181 caggaggcgt ggaagaagct aggaagttag cggcctctta cacagaaaag gctttgaagg
     3241 aaataaagaa attacctgcc acctctcttc gaacaaaaga gaatctcttt tcccttaccc
     3301 aactgatttt ggatcgcaaa gactgaatag ggaaaggttc gatacgtaaa taaatttctc
     3361 agaaggaaat ctacgtgttt tttgacaaat tgaattgtag ttcttgtatt tatgataaaa
     3421 agcaaaaata aagagcaacg tccgttagcg acgttgctct tttttatctt gaatattttt
     3481 ttaccttttc gatttcttat aactcataaa agtgattctt aaaccgatta caagtaatat
     3541 aacgcctgcg agtgttatcc aattattcga ttgacttcca gttttaggaa gtgctctttt
     3601 tgatttgttt ccattgtcat tgcttgtttt attagttaag ttaaagttcg tcctgttatc
     3661 cgaacttgtt tttgagaagg agacacttgc atgacgtgta tcgcttgtgg tagacgacgt
     3721 gtctgtacta tcgcttgaag ttgatgaatc tgagctatca cttgtggtag aggatgtatc
     3781 tgtactgtca cttgaagctg acgtgtccgt gctatcactt gtagtggtag atgaatctga
     3841 gctatcactt gtggtagagg tagtatctgt actgtcactt gaagctgacg tgtccgtgct
     3901 atcacttgtt gtggtagatg tatccgtact gtcgcttgaa gctgacgtgt ccgtgct
//
LOCUS       DACEZN010000194         3936 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEZN010000194 DACEZN010000000
VERSION     DACEZN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.194"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..105)
                     /locus_tag="IXH60_002597"
     CDS             complement(<1..105)
                     /locus_tag="IXH60_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1991481.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIA"
     gene            complement(137..694)
                     /locus_tag="IXH60_002598"
     CDS             complement(137..694)
                     /locus_tag="IXH60_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1991482.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(707..895)
                     /locus_tag="IXH60_002599"
     CDS             complement(707..895)
                     /locus_tag="IXH60_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1991483.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(908..1474)
                     /gene="amaP"
                     /locus_tag="IXH60_002600"
     CDS             complement(908..1474)
                     /gene="amaP"
                     /locus_tag="IXH60_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1991484.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2704..3177
                     /locus_tag="IXH60_002601"
     CDS             2704..3177
                     /locus_tag="IXH60_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1991485.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3186..3413
                     /locus_tag="IXH60_002602"
     CDS             3186..3413
                     /locus_tag="IXH60_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1991486.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         1179 a          704 c          591 g         1462 t
ORIGIN      
        1 agcaatgatg tttgcgatga tccccattga tgatccttta ttcgtgattg ctcctgcaat
       61 tgctccaatg ataccgccga cgattaatgc ccaaataaat cccattttaa attcctccta
      121 actgacttct tacttcttat tgtacacgtg gttcgttatt ttctttgact ttttgtgcac
      181 ctttgcctac agcagatttt gctttatctg tttggcgtga agcaaattgt cctgtggatt
      241 ttgctgcttc tgttactttg tcttgtactg tttcttgatc tttttggtat tcttcttgtg
      301 ttttgatgtc tgcaacattc acattcactt cgataacatc taaatgtgtc atattgttta
      361 cttcagtact gatcaattct ttgatttggt tgtaaatatc ttcgatgtct ttaccgtatt
      421 ctacgatcac atctagatcc acagctactt gtttcttacc tacttctgta ttgatacctg
      481 ctgttacgtt atctgtgttg actaattttt cagccatatt tgagaaaaat ccgccatcta
      541 atgtcaatag accatctaca tctttcaatg cataaccgat gattttttgg atgactttgt
      601 cttcataagt caattcacca ttcatcatgc ggttttctcc tttttcattt gccaaaactg
      661 atccatttgt tgtattcatg tttttgtttt ccatctttgt ttcctcctat gatttaaaaa
      721 attgatcaac tattcctgac tgtttcaaat aaaaaccgat tcctgcgcca attgctgtca
      781 tgattatgat caacaatgtc ttgaaaaatc caatcgttaa ccataaaaca gctagcagta
      841 gtccaagacc cccaaagata atagggagct tgtatgctga aaatagttct ttcattattt
      901 ggccctccta ttccactcga gagtgcttgt agtctcttgt atcttcttgt tcttcataac
      961 cgctgtatgt gactgccact ttgaccgttt cttttgttcc gagtacttta gttacttctt
     1021 cttcgatatc ttgcatcaat acacctgttt tccctactaa tgaagaagtt cgtttcaagt
     1081 ctccgttgac atggatatgg attcgatttt tcgttgaacg tatccgtact ttaggtgaat
     1141 gaatgaactc ttcttcgtgt aaatgggaac gtaccaaccc ttcgatcgct tttttgtcga
     1201 ttgttagttt gccatcttcc cgtttcataa cgaaagttcc tcttgacttt ggatagaaaa
     1261 gaacgactag caagatgata atagcaagaa cggcaaatac tacagctgcc cagaaaatat
     1321 attgttgcat gtagtaattc gttaaagtaa agaagcgaaa tcgttccaat tgttcaggca
     1381 taatatacgg cgcattgatc aacgcaatcg ctagaaatac tgaaagtagc agtaaactca
     1441 tgataacacc gattattttt aaccccttat tcattttcca cctcctataa acttattttt
     1501 accctgtaaa taactctacc gacaatcaca gtatagatag cgaacagtta gcatagcatc
     1561 agtaaataaa gcgctaccaa acattgctgg atcataacgg cgttttattg ccagtctgat
     1621 actgaaataa caattgctta cttattctct atttactttt gattgctggc tatacttaaa
     1681 ggatattatt caagattaaa aattgcaaag attatgctca gttattatta caagattcta
     1741 tttatttatt aaattttttc ggagaattaa aaaatacaat aaataacttt agttacttta
     1801 atgcgttctt actttatttt tatattatca tttttcttat tcacttcttt ggtttatact
     1861 attcttataa ataaaaacga ggtatgtcct atgatgaaaa gattgtacta ttcattgatc
     1921 ataacgattg gctatttgat tgtatcaaat ctaggaaata tggtatttgg tatcagtaaa
     1981 gaattcagtt ggactactac attatgggaa tctctattct ttttcatctt cgtatttttg
     2041 ttacaaaatt atcgaaaaaa ataaacatgt gccatctatc tgttggcaca tgttttagga
     2101 tttatggtac tttttattcg gcaatccatt ttatttttct acttcttctt aatacctcta
     2161 acacttttga tttataaact aatccaatgc atctacttgc gcttattaag ctatttcagc
     2221 tagatagtac caccccatgc gtcaatgctt gattttcctt ttgtataaca ttgtataagc
     2281 aataaaggac aaataagtgg tgattacacc aaaccattga ataagaaaaa ttctgatacg
     2341 taaataaaca tgaacttgaa aaaattaatg aatcggttag ctactacgta gttgtctttt
     2401 ttctcataca gttctgttaa cttacacttc tcttcgctag ataataaaaa atcctccaac
     2461 tgtattgtcc aatataatcc caggcatcta aactagcatt atagaagaga cacacataga
     2521 atctaaaata actggtttta aatgtttctt acaatcaaaa aaagctgtta aatgcactcc
     2581 acaccttcat actctatcat tttttataaa cgatatattt ttattgtttt tcgataaaaa
     2641 atagttgttt tacaataatt attctgctat attttagcta tcaaagataa aagagggata
     2701 aaaatgaaat ttaaaacatt gctattaaaa tttgtcgttt taggtataag tgccgtattt
     2761 attttgttcg gcgtactttt ttatactcag atcgctacaa gcgaaaaagg attcaatttt
     2821 gattggcaga ccacattatt tgtttctgtc atttactttg cccttctcct agggatcgtt
     2881 attgcttatt tcttattgaa actattgaaa aaaatcgaag ataaccaagc tttttccact
     2941 caaagcttaa acatactaaa aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt
     3001 tttggcttac ttcctaaatt tatgaaagta gcggatttag acgatgctcc aggtttgatg
     3061 ttggtaggaa tcgctgtcgt attcattcct tttgcagtat acacattaac aagcgtgctg
     3121 gaaaaactgc ttgaacatgc gattttactt aagacagatc atgatttgac agtgtgaggt
     3181 gcgacatggc aattatcgtt aatttagatg tagtattagc tcaaaaaaaa atgagtgtga
     3241 ctgaattgag tgagaaagtg ggcattacga tggcaaacat ctctattcta aaaaatggaa
     3301 aagcaaaagc catccgtttt tccactctcg aaaaaatctg ccaagttttg gagtgtcaac
     3361 caggtgattt gttgacctat aaaaatgact cgaatactat accagaagaa taaaataaag
     3421 tagaacaaac agtaaataaa aaataatctg aatgatagat gtgtgagact tttagaagtt
     3481 atttacgtct attattcaga ttttttattt cgattgaata ccgttttatc tataaataag
     3541 atatgtactg actcatacaa agggggcctt ttcttctcca tgtactatca cttttcctga
     3601 tacaactggt ttcctgttac gttgtagttg gatctgcttt tagtataata ctttattcat
     3661 tccaccagac tgatagcagt ttgagcagtc taaataatca tgtagtatta caggccattc
     3721 tgtttcatac cttgaagtgc ttaaaacttc agctaatggc ccattgtgtt aactcttact
     3781 ccattctttt ttagtaagct cccatatata ctgtaaagcc ttctatatat tatgtcatgt
     3841 tgctttttaa catttactat gtagttttga taaaatgatt tgaataacag tgatggacaa
     3901 ttctttttta tttttaacat tattttgata ataatg
//
LOCUS       DACEZN010000195         3878 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEZN010000195 DACEZN010000000
VERSION     DACEZN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.195"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(718..3867)
                     /locus_tag="IXH60_002603"
     CDS             complement(718..3867)
                     /locus_tag="IXH60_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1991487.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DACEZN010000196         3871 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEZN010000196 DACEZN010000000
VERSION     DACEZN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.196"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            7..1398
                     /locus_tag="IXH60_002604"
     CDS             7..1398
                     /locus_tag="IXH60_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1991488.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="IXH60_002605"
     CDS             1414..2454
                     /locus_tag="IXH60_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1991489.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="IXH60_002606"
     CDS             2473..3558
                     /locus_tag="IXH60_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ1991490.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..>3871
                     /locus_tag="IXH60_002607"
     CDS             3591..>3871
                     /locus_tag="IXH60_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1991491.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLS"
BASE COUNT         1395 a          545 c          715 g         1216 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat c
//
LOCUS       DACEZN010000197         3820 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEZN010000197 DACEZN010000000
VERSION     DACEZN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.197"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..603
                     /locus_tag="IXH60_002608"
     CDS             <1..603
                     /locus_tag="IXH60_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1991492.1"
                     /translation="FNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMGPLAGI
                     GDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKESGASLL
                     TQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQVMPGL
                     LPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            613..1104
                     /locus_tag="IXH60_002609"
     CDS             613..1104
                     /locus_tag="IXH60_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1991493.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            1122..1532
                     /locus_tag="IXH60_002610"
     CDS             1122..1532
                     /locus_tag="IXH60_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1991494.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            1536..2936
                     /locus_tag="IXH60_002611"
     CDS             1536..2936
                     /locus_tag="IXH60_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1991495.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            2959..3333
                     /locus_tag="IXH60_002612"
     CDS             2959..3333
                     /locus_tag="IXH60_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1991496.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         1341 a          490 c          784 g         1205 t
ORIGIN      
        1 tttaacacta cgccgtatac agcgccttac attattggta tgaatgttgg aattgaaaaa
       61 gaagaaaaaa ataagtcttt ggaagcagta agtgctctga aaacaggtct aatgggtcct
      121 ttagcgggaa ttggagatag tgtatttgta gttattccat ggactatttt tggagcaatt
      181 gcagctaata tggcattgga aggtagtttt gttggaatta ttttgtggat actggcatca
      241 attttattaa ggattactgc ttatccttta taccttgctg ggaaagaatc gggtgcgagc
      301 ctactaactc aaattgaaac aaaattaaag tctattactg atgcagtatc aattttaggg
      361 ttaatggtgg tcggtgcact cattgccaca gttgtaaaag tgaatgttgg ctttgaattt
      421 aaacaaggcg acttaacaat gaccggaaat gaaatctttg atcaagttat gcctggttta
      481 ttacctgcat gtgtagtagc aattgtgtat tatttgttag gacgaaaggt taaaccagtt
      541 tatgtaattc tattaatctt agtagtttct attgcgttaa gagcgttaaa tattttgaag
      601 taggtgttga atatggatga aaaaatgaaa ggtattatac atgttcgtat tgatgatcga
      661 atgattcatg gacaagtagc aacacaatgg acaaatcaat tgagtgctaa cagaattatg
      721 gttattaata atgaagtagc caatgatgac gtaaaaaaag ctgtagttcg cctcgctgca
      781 ccacctaatg ttagaacttc aataattaca agggaagtag cagttaaaaa tattctttct
      841 ggtaaatatg aaggacaaaa agttcttatt gttgcagttt ctccaatcga tgttaaattt
      901 ttgattgata acggtttacc aataacaagt gtaaacgtag gaaatttatc aagaaggaat
      961 ggaacagaac gcatccgtcc gacgataaat attactgaag aagagagaga ggcttttaaa
     1021 gaactaatta gtgatggagt tgaagtgaca gtgatacaaa ctcctagaga ttcaaaagaa
     1081 tttttaaagg attttattta ttaagaacag aggatgagtg gatggagttt ataattacat
     1141 ctcatggaaa tattgccatt ggatttgtag atacattaaa aatgtttttt ggcgataatt
     1201 taaattgtca tgtggtaaca cttggagatg caggcattga agaattccgt gaaaacattg
     1261 aaaaaatagt ttctcaagta aaaaatcaag aagttatggt atttgctgac ttgattggtg
     1321 gaacaccttt caacgaattt gctaaaagaa gtgaagtatt tcaaaatggg tttcaacttc
     1381 ttggtggcat gaatttagga attttgattg aggctgtcaa tttgagatta caaggaaaaa
     1441 aaagcgatga tatcatcgaa aatctaagaa atatgaatac aattgcgtgt cttgcagata
     1501 tgcaaagaca tactaacgaa gaggatgagt gataaatgca aaaaattttt tacaagccta
     1561 aagatttttg gtttggggat tgcatgcctt tttataaaga tggaactttt tatctatttc
     1621 atcaacgaga tacgagaaat cctaaacctt taactgatcc ttttgggtgg tcactagtta
     1681 caaccactga ttttgtgaaa tttgaagaat acggggaagt aattcatcgt ggtggcaacg
     1741 atgacattga tcaatacatt tacgctggaa gtattgtcca aggaatcaat gagtatttag
     1801 ctttgtatac tggacataat cgcaaagcta agttggatgg aaaaacttca gaagtgttaa
     1861 tgattgcaag ttctaaagat ttaattcatt ggaaaaagga tggcgaagca gcaaaacttg
     1921 ttcctcaaga aggatatgat aagaatgatt ggcgcgatcc ttttgcaatt tttgatcaag
     1981 atcatggaaa atacgttttg atactaggag ctagaaaacc agcaagtaag aaaaagccga
     2041 cggggagatt ggtctatttt gaatccgaag atttaaaaga ttggaatttc aaaggagact
     2101 actgggctaa taatgaattt aacatgattg aaatgcctga tatatttgaa attgatggaa
     2161 cgtggtattt acttttttca gagtattgtg aagataaaaa gactaaatat cgagtcggag
     2221 caggattatt ttcagattgg aaatcaccag tagacgaagc tttcgacgga aaagcctatt
     2281 atgcagcaag aacagtcgga actaatgaaa ctggccgttt tctaactggc tgggtggcaa
     2341 caaaagaagg ggagaacgat caaaataact gggattgggg cggtgcactt gttccacatc
     2401 aaattattca aaagacagac aaaactcttg gtgttaaact gcctgaaaaa ataattgaga
     2461 attttaaaga aatcactcca ttttcaagtt ttaagttaga gaaagaattt ggaaaaaaag
     2521 aaagagtaat tgttcctaaa atcgaagatt attttctatt tgaaacaact ttgactatta
     2581 catcgttaac aaaggagttt gctttaaaat tatttgtaga tagtgatact caagaaggat
     2641 acgaatttcg tatttcattg attaataatc agcttacttt cggaaaaact ccgaactatc
     2701 cttggcctca aatgtttgat aaggggctgc aacgaccact atttttggaa ataggcaaag
     2761 aatatgaact aaagttaatt gttgatgata cgatagctgt tttatatatt gataatgttg
     2821 ctttaagtac tagaatgtat gataaagtcg gaacagcatt aagtatgact gttacggagg
     2881 gagcaatcaa ggtagataaa ctagactatt caataaatta tgaatgtgga ttttaaaaca
     2941 attgaaggga tgatgtaagt gaaattaaat ggtttacaac atattggaat tccaacaaaa
     3001 gattttggag cttcaaaaat cttttatgaa tccttagggt ttagtctgat taatgaagaa
     3061 atgaatgtgg gaagtaaagt cggctttttc gagttgaatg gaacgataat agaaatatgg
     3121 gaagataaaa ctactgagtg tagaggagcc attgatcatc tagcattaga tacggatgac
     3181 atagatgagg tattcaattc aattcaagat ttaggatatg aattattaga taaaaatatt
     3241 atggaattac cgttctggga aaatggaatc cgttacttta atttctacgg accaaatcat
     3301 gagattatag agataagcca aatgaagaaa taaatagata acgaagtgct ctaagttggt
     3361 atgaacgaaa agtacttcat tttttggtat cagcatttca atagtttaat gagaatttac
     3421 tatttcaaat taattcaaag tgacagtatt ttttacttat ttggtttcaa attttgtaat
     3481 ccttcctatt tattggaata cactccacat tttgtataca agtcatatca tgttttagca
     3541 aagctcttac caagcaaagt tataatgatg ttttccaaag tttgatcttt cgatggaggc
     3601 taacacatat tttttgcagg acagccaaaa ttagatgtat agaatagagt ctgtgaggcg
     3661 tatagacagt ttattgtctg tatgcctttt tttattttga aaaattttta gtatttttcg
     3721 aaaaatgctc actcctagag taagcgcccc atagaacagt accttactaa caaatgcacc
     3781 tcaagatata cttgagctat caagaagtct ggaggttttc
//
LOCUS       DACEZN010000198         3818 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEZN010000198 DACEZN010000000
VERSION     DACEZN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.198"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..210)
                     /locus_tag="IXH60_002613"
     CDS             complement(<1..210)
                     /locus_tag="IXH60_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1991497.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="IXH60_002614"
     CDS             complement(309..623)
                     /locus_tag="IXH60_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991498.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..1185)
                     /locus_tag="IXH60_002615"
     CDS             complement(691..1185)
                     /locus_tag="IXH60_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991499.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1178..1492)
                     /locus_tag="IXH60_002616"
     CDS             complement(1178..1492)
                     /locus_tag="IXH60_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991500.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1708..3099)
                     /locus_tag="IXH60_002617"
     CDS             complement(1708..3099)
                     /locus_tag="IXH60_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1991501.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="IXH60_002618"
     CDS             complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="IXH60_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1991502.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
BASE COUNT         1115 a          717 c          509 g         1477 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttctgta tcatttataa gtgtataggt cacttgtaat acacgatcat gttgtgcaaa
      961 ttcatttctt ttttcggtca cttcaatatt tttaaatgtt agttcaaccc catctgcctg
     1021 ataagtttca cctattttta aatcttttgt tacaattttt attggctttt cttctggaaa
     1081 atgtctggct ttaattgtta gataacttcc aaaaagaatc aatcctagta gcactaaaag
     1141 aattccttta acgagccact gaaacgggaa gtaaacttta aacatctttt ttctcctccg
     1201 ttttgtattt gttttttaga gaatacgata acttttgatc aatgtcatcc agcttttcaa
     1261 ttacttcatt acttatcatt gattctagtt ctgaaatttt ttgttcaaat ttaatgcttt
     1321 caggatcatt aaatttatta tttgatggac tagctaacac tggtaaattt gaaaccataa
     1381 cacgaaacat atttgccttt gaaccaaaaa ttcctttgtt ttggttgtaa aaattgttga
     1441 atatttcttt agtttcttct gaagccgaga ctttaaaata tggatagttc atttttttct
     1501 tccacctttc tttttttggg agggagtctg tagggagctt gctccctcag agatttaccc
     1561 gccatctgac ttgccagaaa gtagcctagc gtgagcgggg cggattgact ggcaagtaga
     1621 tgggtagctg gcgtatgtcc taaaaactgc ttctttccaa gcaactcttc ataggcaaaa
     1681 aactggaaag tccactaaca agcccattca gaattccatt tcaaaatttt gttgcctttt
     1741 ttgctgattt tcaatcattt gttgtgtgaa ttcataatcc tttttagcac gatacatttt
     1801 tacatcgtca ttcattatgc gactgatttg atagtgaatc tgtttagcag aaggtgtatg
     1861 agaacaattt tcaaaaccct ttgccggtag aaataactct ttctgatcag tctttttaat
     1921 agaaattgtt ttacttttcg cactacgtaa agtttgagga ttcacatccg tatgaagttt
     1981 aatcgtgtat tcttgatcat cttctaaaga cattgtattt acaaaatgct tgtctacctc
     2041 tcgcaagaag tcatcatatt taatgaattt ttcatcgcct tcacggtcgc taaattgctt
     2101 ttgtgcgatg gaatctaatt ccacaaacgg tacatttaac tcaccagttt tgtaatttac
     2161 tccactaatt agcgacataa cttgctctct ttccatatta aaaaaatttt ttatatgtga
     2221 ttcttctaaa ttcttttcaa gttgttcaga aacagattca aatgaatatc ttttccctaa
     2281 ctttcttgca ctaaaagtac gttcgacata ctgacttttt tcttttctag attgtttatc
     2341 tcttcctaaa gtaattaact cacctctacg ctcatagaaa tttacacctt gtttgtaaag
     2401 aacatagcca agtttgttat ttgtttttga aaattgataa acttcaatgt tatattcttt
     2461 tgccaaatga tccgaaaaat cttcaaaaga attcacttcg catttttcca acgttgcttc
     2521 gatcgcattt tgcacaaact cttgataatt aatttttcca tatttcatct tagaaggatc
     2581 gtttgaataa agattggttc tcttttctaa gttttcatag tactcattat tcactgattc
     2641 aagaccatgt tcattgagga cttgatcgtt aatttttcgc attagttcta aatctttagg
     2701 gtcaatacgc aaccttttac atgtttccat gttaatatta ttaatgacaa gatgactatg
     2761 aaaatgatct ttatcattgt gacttgcaac aatgatttgc gaatttggaa aagctttctc
     2821 tgcaaattcg acagctattt cgtgcatttt atcaatactt aactctttag aagtactctt
     2881 atcaaacgaa tgaataacat gaaacgcttg tgctatatct ttagatttat tcatcttggt
     2941 acgtactaaa ttcatttctt gcagtgcttc ctcactatca tcactacaat ttattcctga
     3001 gactgcaaca catttttctt tttttgaagg attagctgca tattgtaata gcgaggataa
     3061 atttttcttg cgtcctttcg atgttatagt agctcccaaa gttgtttcac ctcatctttt
     3121 aaacttgcaa taatctgaat tgagtgaatt aaattccgat caactttttg ggaatactct
     3181 tctttcgtac ccggtctagt ttgcttttct atccaatatt cgaaatattc tttttctact
     3241 tctgataatt taaaaaaaga gggggcactc cgattggact ccaaaaaaat gtcatctgga
     3301 taactaatca tcaacttttt tgctatgcta ttcgcaagtt catctttaaa aatgttgtaa
     3361 cggctagcat caaaactaaa atgtaggctc ttttcttttt tatcttcaga ttcttctcta
     3421 aaatatttag caatgatatt taatgccttt accgcttgat tcaaattgtt accaatttga
     3481 tttaactgtt tggttaaaac taccctatca tcttcagcta acaacggcgg ttcatatttg
     3541 agtcccttta accgctcacg gcaaacgtct gctctagaaa ttcccctttg ttttgcaatc
     3601 aatgtcagtt caagataagt ctttttatcg acacgaaaat ttaaggcctc actatgtttg
     3661 cgattttttt cgtacatttt ttgtttatca tccattgatt atcccctcac atttttttct
     3721 agagagcaac atttgaaatt gctctctatt tcatttttca tctttaattc ttcttgaatc
     3781 atcagttcaa tataattagt gaccgtgatt ttttcatt
//
LOCUS       DACEZN010000199         3807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEZN010000199 DACEZN010000000
VERSION     DACEZN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.199"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            94..2193
                     /locus_tag="IXH60_002619"
                     /pseudo
     CDS             94..2193
                     /locus_tag="IXH60_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
     gene            2389..3252
                     /locus_tag="IXH60_002620"
     CDS             2389..3252
                     /locus_tag="IXH60_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1991503.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3357..>3807
                     /locus_tag="IXH60_002621"
     CDS             3357..>3807
                     /locus_tag="IXH60_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1991504.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT         1521 a          636 c          771 g          879 t
ORIGIN      
        1 agtaaatcct aaaaataagc tattggcgtg acgtatagaa tatgatagat taaatggtag
       61 cgtaaaccta gtaaaggagc cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg
      121 aaaaaaatgg agcagaatac tatctgtcca gactgcggtc atcaaataac tgcagaagat
      181 cagcaaataa aagaatcaat tgaagaaaca aatacagatg cagctatgca ggataaatca
      241 actgaagatg cagtctttcc tgatacagaa ttaaacgatc caatcgaatg gtcggaattg
      301 aaagatttac ctctagaatc tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa
      361 gaaccacctg ctcacgatca agtaactaat atagagaaaa atgaacagaa gaaagcaact
      421 gttcatgaga cttcggaaga atcaggaaag aatcaagaag aaacgcttga atcaaaacaa
      481 aaaagaagag tagctgaact gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta
      541 tccgcttata tcaaagcaca tcgtgaagat acaaaagaag agcacgcgaa agagctgctc
      601 aaaatgatca gtgaaaaaat tgcacaggaa aacaaagtag agccagaaaa ggaacaagaa
      661 actgaaaaag actctgatag agaaaacgcc acaggtaaag aaacagtaaa aataaaagac
      721 gtaaaaacgg atatcacaga aaaaactgaa gtatttactg aaaaacaatt agaaaaggaa
      781 aaaacgaata aactgccgga agcagagaaa aaagaaatac tcgaaaacac agacaaaaag
      841 tcaacagagc ccgatgaaat aaatagttaa tccgatgctg aaaatcaatc tgtaagcaaa
      901 gagggtactg atcttgaagc aaaaatagaa aaagaagaga accagcctaa gctgtttgaa
      961 gcagaagcga aatcaataac aatcgtgcca gatactgaaa aaacacatca agaatcaaac
     1021 ttagaaaaaa cagatccaga taaagcgcaa aagaaaaaat caaaaaaagt accttatctc
     1081 gttttagcag cagtacttct actcggtgga ggaagctggg cttattatga ccatcatcaa
     1141 aaagtagaag ccgaagtagc agcagaagca aaaagacagc aacaaatgga agaacttcat
     1201 gagaaacttg ctgcctttta tacagatgat caccatcaat ttattcgtgc agatatgatt
     1261 catcaagatt tatcaaaact aaaaaatgat ttatccagtc ttaagaatga aaaaggatat
     1321 tcagagttag aaacaactta ccaagatatc caatctaaga tccgagatat ccaaaaagtg
     1381 aatgagtggt tcatagcacc tgccatcgtc gatgatcatc tagcagatga catcaagttg
     1441 aaagcagata aagagatcca agcaatcgaa acggataaca cagattttgg aaaattgatt
     1501 caacaagcac aaacagaagc taaaaatcaa tatgctgaat tgcaaacagc taaagaaaaa
     1561 acagctatag tctttaaaga cggaaaagta gtggattcag caacacgcga ccaatacaag
     1621 acggcaaaag aagcagtaga aaaagtgaaa aatacagatc tatctgctca gttgaatgaa
     1681 caattgaaac aagtagataa gatgctcact gataaagaga aaaaagaaga agaggaaaag
     1741 aaaaaagcag aagaagcgaa aaaagcagct gaagaacaag caaaacaagc cgctcaagca
     1801 gcagctgcag ctcaagccgc tcaacaagct gcggtcgcac aagcagcaca gcaagaagcg
     1861 gcatcctcga ccccttcctc ttcaaactca gcgaatcagc caatcaaatc tgccagagca
     1921 agtgatattg ccgatgcatc taatccagca tggacgtggg caccaggtgt ccaaagcaaa
     1981 gtgattgcta catgtatcca acgtggatat atcgttgaag gaggttacag attagaaaaa
     2041 gccagaatcg aaaatgggga aggctattat aatctatatg ccacttcaac aaaatctgca
     2101 ttgatgaaag gcattggaga aagtgctttg ccattttaca tcgtaaccat caactgtaaa
     2161 actggatggt tcggaggaaa tgggagcaat taattttagg caaatgaaag aggctgtgag
     2221 cctgccgttt aacaccgttt attcgaaaaa ggggagtatg acacaactgt tgtcatactc
     2281 cttttttcac ctttccaaac caaggctcag ctattcaagt agataacatt atgttatgat
     2341 agcaaagaaa tgtattaaga aaacctttca ctatagaggg agaaaaaaat gaacgattta
     2401 ttagcaacta tatttacagg tattattatt gatgaaaatg aaaaaaacta ctttgtccaa
     2461 aaaaacggac aaacctttgg cttaagcaaa tcagaaggtg aacacaaaat cggtgaagca
     2521 gttgaaggat tcggttacca aaatcaaaaa catgaaaatc ggatcactac acaaattcct
     2581 aaaagccgta tcggtcatta cgcttttgga atcgttactg ctacacgaaa agatttaggt
     2641 gcgtttgtcg atatcggtct tccagataaa gactttgtcg tatctcttga tgaattgccg
     2701 acaatgacag agctatggcc taaaaaaggg gatcgcttga tgatttccct tagagttgat
     2761 gcaaaagacc gtatttgggg cagtttagca gatgaaaaaa tattcaaatc tttaagcaaa
     2821 tatggaacag aagaaatgaa aaacaaagat gtgataggaa cagcataccg cttgaaatta
     2881 acaggtacgt acgttttaac ggaagatttc tatcttgggt ttatccatcc ttctgaacga
     2941 tataatgaac ctcgtctagg cgagcagata aaaggtcgag tgattggtgt ccgaccagac
     3001 ggtgtactga atctttcatt gaagccaaga gcatatgaag ctatatcaga tgatgcacaa
     3061 atgattttaa ccattttaga acgttctgca gatcagcaaa tcaactttac agataaatcg
     3121 gatccagaag aaatcatgaa agcttttggt atcagcaaag gagcatttaa gcgcgcatta
     3181 ggtaatcttt tgaaacaagg attgattaca caagaaaacg gtgtgacaaa actagtcaaa
     3241 aaatctaatt gagaatccct tgcatgtttt tcttaactag cttataataa ttttaattaa
     3301 catagtgtta tatctttttt tatttgtaat tattataaat tgttggggtt gagtaaatgg
     3361 tcatgaatgc tatttcggcg atgaaaaaaa caaaacagca gctacatgaa gcggggttta
     3421 agctgacacc gcaaagagaa gctactctgc ttgtgctttt ggaaaatgag aaagagcatt
     3481 tatctgcaga agaaatcttc tttttagtga aaaaaaagag tccggaaatt ggacttgcta
     3541 ctgtttatcg aacccttgaa atcttgacag aattgaaaat tgtcgataaa gtaagtttta
     3601 atgatggatt agcccgttat gatttgagaa aagaaggcgc caaacatttc catcatcatt
     3661 tactatgctt gaactgcggc acgatcgagg aagtagaaga ggatctttta cttgaagtgg
     3721 agcaagtagt agaagaacgt tatcattttt tagtaaaaga tcatcggcta acgttccatg
     3781 gaatctgtca ggaatgctat gaaaaag
//
LOCUS       DACEZN010000200         3785 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEZN010000200 DACEZN010000000
VERSION     DACEZN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.200"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..171
                     /locus_tag="IXH60_002622"
     CDS             <1..171
                     /locus_tag="IXH60_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1991505.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            346..642
                     /locus_tag="IXH60_002623"
     CDS             346..642
                     /locus_tag="IXH60_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991506.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="IXH60_002624"
     CDS             649..2601
                     /locus_tag="IXH60_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1991507.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IXH60_002625"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IXH60_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1991508.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="IXH60_002626"
     CDS             3400..>3785
                     /locus_tag="IXH60_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1991509.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DACEZN010000201         3776 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEZN010000201 DACEZN010000000
VERSION     DACEZN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.201"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(72..145)
                     /locus_tag="IXH60_002627"
     tRNA            complement(72..145)
                     /locus_tag="IXH60_002627"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Ile,seq:cat)
     gene            332..655
                     /locus_tag="IXH60_002628"
     CDS             332..655
                     /locus_tag="IXH60_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991510.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            819..1073
                     /locus_tag="IXH60_002629"
     CDS             819..1073
                     /locus_tag="IXH60_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991511.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            1143..1388
                     /locus_tag="IXH60_002630"
     CDS             1143..1388
                     /locus_tag="IXH60_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1991512.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1485..1829
                     /locus_tag="IXH60_002631"
     CDS             1485..1829
                     /locus_tag="IXH60_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991513.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1890..2453)
                     /locus_tag="IXH60_002632"
     CDS             complement(1890..2453)
                     /locus_tag="IXH60_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991514.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(3021..3221)
                     /locus_tag="IXH60_002633"
     CDS             complement(3021..3221)
                     /locus_tag="IXH60_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1991515.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1139 a          660 c          590 g         1387 t
ORIGIN      
        1 aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc
       61 acaacctctt atgtaccttg taggactcga acctacgacc ggacggttat gagccgtctg
      121 ctctaaccaa ctgagctaaa ggtacatcat aaataaaatt tatttttgtt acttttcaac
      181 tatagcacga atgacaaact ctcacaagtt tcaaaatata agtaaaataa tattgttatt
      241 cttatttctt ttacttgtgt cataactatt ttgttacttt aatttttatt tgcttatttt
      301 agaaaaaatg ttattctttt atttattctc aatgttagga gtgattattt tgccatttat
      361 tgcgatttta ttcgatttag ttgcggcagc agcttatttt ctccaattaa attatcaaac
      421 ctcaggaatt ctgtttattg gaatgatttt tcaaggtgtg attacccttt tgcttcttgt
      481 catgatgctc agctataaag gaaaaagata tgcccgtatt caaacaaaag tatttgttaa
      541 atatgtttcc attcgctatg ggatcatcat tctttccttt ttagtgaatg cgatcgtcct
      601 ctttttatat gtattgaatt acttagatat caatccattg attttttctc gatagataag
      661 acacattcag cggaacagtt cctgcttatc agcagaaaac tgttctgctt tttcattttt
      721 cattgatcta ttcttctttt attttcctag catttcattt gtgatgtatg caataacatg
      781 ttatctttta ttatatgaat cgttgaggag tccgttacat gattacgttg gtgagagttt
      841 tgttttgggt gcctgcggtc gcactggtcg caagtattgt ttatcttatg aattggaaca
      901 aagaacggtt ttatttagct attttgactc ttccggctat ctactttatg tggaaagttt
      961 ttaattacaa ctactttgaa cccgatagtg tttttataga agaattatca ggactggttt
     1021 tgtctttatt gatcgtcatt ctttatttga tacgtttaaa taaaaaacat tgagtactat
     1081 tgattgcatg tgtagctttc tatagtcatt ataactattc tactataatg aaaggtgatt
     1141 aaatgagtat cgaagaagct attttactgg atactttacg ttgtcgtctg aatttcttaa
     1201 gaactggccg atctttatct caagtagctg tttctagcgc gttaagtatt cctttattca
     1261 aatatcgtaa gtacgaagaa ggagaagaaa ttccaagcat cattgaatta atctatatcg
     1321 ctgactttta cgatatttcc gttgattacc taattggtcg ctcagaaaca gcaagcaaac
     1381 tgaagtgatt ttttccagcc ttggtgctgg ttttttcatt taacgtctaa ggttaacaat
     1441 ctatatataa aggagacatt tctatgtgat tatggcatga aaaattgatt tcaaagcttc
     1501 ctcgtcaaca gcttttagga caacatcggg aatgctgtgc attaagagga aatggctggg
     1561 gaaagaaaca tgctactgtg gattatgttt ttcactattc tccttataga ttattccaat
     1621 atcatcagct tgtcatggat gaaatggaaa aacgtggtta cagcccagca ccagagtgga
     1681 aaaatcctat gtatcgtggc aaatcatgcg aagcattttt tgatttacct actgtagagc
     1741 gcaacgtgcc gatttatccg gaacacgatg aagtttatct agcagaatgt ttagcaaatt
     1801 tggagcaaaa aggaatttat cttacctaat ataactagaa aagccttcta tctcctctta
     1861 cttcattaaa aagctttaac catcttagat caagctaaaa tgtcgtaagt tttagggtta
     1921 tcttcatcct cacgtgcaaa actaaaagcg tactctagtt ttggttcatc gccaacttcc
     1981 ctagacataa atgaacatct cctgacgtgg tttaaaagta ccgcatcttc gacgtctgta
     2041 tacgatgcac agcacagatt gcttcttttg atctttcttt tgatttttct gttgcttctt
     2101 ctacaccttc ttcgattact acatcgatgc ttacaattgc ttctgcctaa tattcccatg
     2161 catcgtcgat ttaagcctct gtagtcactg tgatcgtttc cacattttaa ctcctctgtc
     2221 gtggcttcag ttgattttgt gatcagttca gttgctaagc cagagtcaga acaacctgcc
     2281 attgctagcg ttaatattga tagtaaaaat agttttttta tcataaggtt ccccccctga
     2341 aaatagacta aaataataac gctttctttt tttgttttca gacgaaaatt aataatttct
     2401 aaatattctt taaaaaagct tataactatt atgccttttt taatatataa cacagttatt
     2461 tttaaaaata agaatgctaa atcactctac aataacggcg aaagcacaaa aaataaaaaa
     2521 attattacgt tattaaaaaa acagatgaaa gatcaggttt tattagatct cattctttca
     2581 tcacttgaag ggatttaatg aactactttt tcctctttgt cgtttttata tattcgtatt
     2641 ccttcaaaca gtaggatagc ctctttcaat gcttgatatt tgaactgtcc gttacttaaa
     2701 aatttagatg tgccgccctc ttcctagaac atattctttt ttactttttc attaattgac
     2761 agaccaatcg aacgaaactg taaatttcga taggataatg attggtcaaa ctcattcatt
     2821 ttcaagacat ctcgattcat attcaagata ggaatctttt tatcaacatc actttattcg
     2881 tattttcctc taccataaaa gcttcacgct ccacaaaatt gttttataca ctactattca
     2941 gcccattcaa cgaccaatga attttttcgt acaaaaaaac cgtcttttct ttcgaaaaga
     3001 cggttaaaag tcaattttta ttaagcttta acaatgtttg ttgcttgagg gccacgttga
     3061 ccatcttcaa catcgtaaga cactgcttgg ccttcttcta aagttttgaa gccgtcacct
     3121 tggatagctg aaaaatgtgc aaatacatca tttccatctt caccagtgat aaagccaaaa
     3181 cctttgtctg cgttgaacca ttttactgta ccgttattca taaataaatt cctcctgata
     3241 cgtatcaatg tacgtttttt cgttgcaata aatatttgat aggcaaaagg agttttatct
     3301 atgtagatat atcgctcaaa ttacgtttca aaatagatta ctcatttaat atatcatgct
     3361 ttttctaata acacaaggat aatgcaatga aatataaaat tcattgtctt ctattgtctt
     3421 cgaatcgaag aaaaagttga ataaccgcga ttgattcact ttttgttttc ttgtttattt
     3481 gcttttatct gcaacttttg actttaaaat ttcttaacta tgaaaacctt tcaattggtg
     3541 gtatacttat aaatggaatt tcgttatcaa cgcgttcgtt gcctttcagc aagtatttaa
     3601 agaaaggaga atggctatga cattctataa caaagatata caatatcgag taaccttgga
     3661 tacagatttg aatctatttg tcgtttttga caaagaaaat accaatcatg tagcgacagg
     3721 tgttacgatc gaacaagcag tgcaagaatt aaaaaaatct gcataaggag tggaaa
//
LOCUS       DACEZN010000202         3767 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEZN010000202 DACEZN010000000
VERSION     DACEZN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.202"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..912
                     /locus_tag="IXH60_002634"
     CDS             <1..912
                     /locus_tag="IXH60_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1991516.1"
                     /translation="VYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTI
                     LSALDELQKVKLLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQK
                     NLIVQNLNYGTNQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKE
                     SHNDDLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWY
                     KKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQK
                     DDYIFASLKNKALELIELQISEGIDKQ"
     gene            988..1206
                     /locus_tag="IXH60_002635"
     CDS             988..1206
                     /locus_tag="IXH60_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991517.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            1193..>3767
                     /locus_tag="IXH60_002636"
     CDS             1193..>3767
                     /locus_tag="IXH60_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991518.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RR"
BASE COUNT         1455 a          508 c          729 g         1075 t
ORIGIN      
        1 gtttatatgt tactaaaaga tagaaataga ctttcattga aaaataactt gtatgatgag
       61 aatcaaaaaa tttttttgat atttgaaatc aaaaatctcg tagaaatttc gggaatgtca
      121 aaaaatacaa tcttatcagc actagatgaa cttcaaaaag ttaaacttct tgtgaaaaga
      181 aaaaatggct tcaataaaag taaaatgcaa atgttcccaa atttttatta tttgttaaag
      241 cctgttctgt gcaaagatga tgtttataaa attaataaga taacccatca agaaaataga
      301 caaaaaaact tgatagttca aaatttgaac tatggaacaa atcaatattc tgttgataat
      361 tcaaaatttg aactatgcga cacatcaatg aaaacaagag acaaaaacaa ttttgctgat
      421 agttcaaaaa atgaactaga attatataag tcttataaag atagtaaaga tattaaagaa
      481 tcacataatg atgatcttct tgaatcactt tcaactaaaa ataataaatt agatgaagaa
      541 ttgatcgaaa catttattga agaaaatctt cttaaacaaa tatggggaga aagtattgtg
      601 aattgcttaa aactggctag caatagtgat tatcgtcaat tcgacaactg gtacaaaaaa
      661 ttcaaatatg cgattagatc tgctgaaaaa gaacaaaaaa ttcaactgaa aattatctat
      721 gaaatttgta ataacaagta ttttgtagat catgttagag aacagctatc aatgacttta
      781 cgtgacttga tacgaagagc aaaaacagat catcgtatca aacaaaaaga cgattatatc
      841 tttgcttctc taaaaaataa agcgttagaa ctaattgaat tgcaaatcag cgaaggaata
      901 gataagcaat aagaatagaa ttgagtcaaa tgaaatggag gggtatctcc acttcatttg
      961 agaactttga atataggagg ggttattttg attaaaaatc gtttaataga tcaagtttgg
     1021 tgtctagatc aaaatacaga tagtctcgaa ttaatcgaga ctatttgctt caacactata
     1081 gttctcgatt taagagctga aaatgataat tgtgtaactc atgtcataat taatcaaaaa
     1141 atggctcaga agctctctga gagcctcaga aaatgggcta agggaggtaa cgatgaaaat
     1201 aattgaaaaa aagattgcca ataaaaaaat aaattggttg gttgttaatt ttcttgttct
     1261 gatagctatg attattttta caagtaatat tgctcatgcg agtattttca attggtttga
     1321 tgacacaaaa agtcaaacag agtttttgaa taattctctt tatagacctt acctaaaaaa
     1381 gtacaatgga gatttcctta gtcagttcgg gatttggctc ggatgggctg tggtaaaagg
     1441 catgtttact gtgacagatt cgattcaaaa tatgattcca gatgtgctgg atttgttcaa
     1501 tttcattgaa agtacagggc taaataatgt ttatcaatca gccgtaaata caattgtcat
     1561 tggattaatg atcctttcat tgatgtttgt tggttacaaa atgattactg gaaaaggaac
     1621 agtggattta aaatcggttg gaatgaacgt agtgatgtct atagcattaa tacttttaat
     1681 gccaaccatg atttctagtg gtattcaatt tgctaaaaca ttttataagg actctactac
     1741 gattacaaat tcagatgacg gagtcgcttg gtcattgata aaacaaggag taacagattt
     1801 agcatatatt aataaaacag atcagtactc aagcattgat aaaacggagg atcgaaataa
     1861 tttaacacga aaaaattttc aacagacgga tctcacacaa gtgttaacgg ataaagtgat
     1921 tgataaattg gaaaaagaaa atccaattgc ggacaatctt cgttatgaat tagttgaaaa
     1981 ttctaataat gaatttgttg ctacaaagtt ttctgacaac ttcttatcaa cattttctga
     2041 tagcttgaaa tcaggctatt accgatacca agcaaatcta tgggggattt caattgggtt
     2101 gacagcgtta gcaatcgcct atgtgttcag cgcgtttgtg atcattacag caatactaga
     2161 acttgcattt aaacgtgtgt taggtgttct tgtttttgca acagatattg agacaggaca
     2221 gcgttcaaaa gttgttctat cagatattct acaatgttac ttaactgttg ggtttcaagg
     2281 atttgggtta tcaatgtttg ctatgtttat caattttctg aactcaggac aaggtatctc
     2341 aacaaatatt tttataaaaa caattgctta tatctgtgcc gtttttgttt taatcaaagg
     2401 cagtggaaca gtcatgagat actttggtgt tgatattgga ctcaaagaag gctatgggca
     2461 acttgcttct gcttttggaa tgggcgcaat gctgtttaga aaaggatcaa atggatttaa
     2521 tcgtgctaaa ggaagcggag aaggagaaga tcgaaaacca gaaaagaatt tcggcgaaac
     2581 tctatcaaaa aaagctggca aaactggaag agctttggga tatgcccatg aacgtggatt
     2641 atccggttta gcttcagatg gagctacaat ggctagtgaa agagcaacaa aaccatttaa
     2701 gagtatgcgt gatatagcta atgataccaa gaataagttt aaagaaggtt tagatgatgg
     2761 tacagtgtcc gctattaata aaaatagcaa acctatgtta gctaaaaata aagaagatga
     2821 aactggaaaa tatgctgatt ccgtgccaac acgtctttca gatagaaaag atggtgctaa
     2881 agttgaaaat gccgatagaa ttatgtctag ttctgaacgt atgcgtgaag caatgaaaaa
     2941 taatgctgaa tcgaataata atcctgcaag cgcaattcag caaaaagtcc aacaagatat
     3001 tgaagaacgt aaaaatgcta tgcacggtca agctaagtct gctgaagaat taattaatca
     3061 gaagcgtcat gaagcgaaat acacgcctga agcaatgaat cgcgaggaaa taatgttaag
     3121 gaaacgtgta gaagggcgga caggcgctaa tattgacgga aaagaagcgt tgaccaaaga
     3181 aagaattcaa gaagctaaaa attcaaatac aggattagaa aaattagtca aagaaaatcc
     3241 acaaaattct acaagtagtc atggtggccg atcagtagat gtccgagaaa atattcaagg
     3301 ttcactgaat ggtcatgttg gacggaatgt tgatgtaaga gagaatttac aaagttcttc
     3361 aaatgctcaa ggtactaaga caattgatgt tcgagaaaat ctgcaaagta gcagtacaag
     3421 tcaaccaaaa acagtagatg ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt
     3481 taaatcagtg gatgtccgag agaatgtgca aggtagcagt gcaagtcaac caaaaacagt
     3541 agatgtccga gaaaatgtac aaagttctac aagtagtcaa ggattcaaaa cagtagatgt
     3601 tcgggagaac attcaaaaag atagtggaga aatgaaagaa aaaacacaaa aaatcaatat
     3661 cgttgaagac agaaagtcag cgaaaaaatt tgattcaaat catgaaacag tgattattga
     3721 tagtgaaatc cgtgaaaatg ataagggatc aaaatcaaga cgaagat
//
LOCUS       DACEZN010000203         3749 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEZN010000203 DACEZN010000000
VERSION     DACEZN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.203"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            498..1538
                     /locus_tag="IXH60_002637"
     CDS             498..1538
                     /locus_tag="IXH60_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1991519.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2362..2694
                     /locus_tag="IXH60_002638"
     CDS             2362..2694
                     /locus_tag="IXH60_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1991520.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3313..3654
                     /locus_tag="IXH60_002639"
     CDS             3313..3654
                     /locus_tag="IXH60_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1991521.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1382 a          487 c          687 g         1193 t
ORIGIN      
        1 atcttattaa ataagttaat tttaatggta gtacataatt attatatata tctgacattg
       61 gtagtactta ttgtattttt tagtagtata taatctattg ttttctagcc taaagccttg
      121 tcttataaga ataatataat atatactact gaaataaggt agtatatatt actagtaatg
      181 gtagtatgtt ttatatgcat aaggtagttc ttaatttatt ttttgttttg acactggtag
      241 tacttaatta tctttaattt taaaactaaa ttttattcat acgtttttct ggtagtattt
      301 aattataaat aatatttatt taaagtagtt cttaatgact ttaaaggtag tacataattc
      361 attttatttc tgattttagg tagtatttaa ttaaaaaaca gttttaatgc gtttgcattt
      421 tttatttatc tttgctattt tcgaaaagag taggtaattt tacataaata aaacaacgtg
      481 aaagtagagg atctacaatg actgatttta aattttttaa ggcggataga gtttacaacg
      541 aattatttta tcaatttcca aaagtcttta ttgtttctga cgaatacaaa aaaatgaaag
      601 attcaactaa gattgcctat atgcttttaa aagcaagatt agagatcgca atcagcaaac
      661 ggcaaatcga tgaagaaggt aatgtttatt ttacttatac gacaaatgaa ctatgtagag
      721 tattaaactg ccaaaaacaa aaagcgatag caatcaaaaa agagttggaa tcctttggtt
      781 tattattaca aaagcagatg ggatttaaca aacagttagg gaaaaataat cctaatagac
      841 tatatctagc agaattaaaa gtctcagaaa atgatatcta cttactcgaa aaatttgata
      901 gagagaatag ggaaaacgtt gataaatcag agggtatgaa aatcataccc accctcgacg
      961 aaaaatcaga cgctgaatcc cttggggctc aagagggtat gaaaatcata ccgtgccaaa
     1021 acgttgataa atcagagggt atgaaaatca taccagaact taataataat atattagaca
     1081 ctaatagaca caatatagac actgaaaaag accgcctaca agatcaattg ttgttagaca
     1141 attttgagac aattatgaca aacgacagca ttgctacgtt tgtccctgaa cgagtattaa
     1201 atttgataaa aacattttct tcaagttaca gtgaagctca aaaaaccgtc cagactattc
     1261 ataatgcaaa gaaaaaagct gaaatagaaa gtggtatttc gatagttttt gaagaactcg
     1321 atagttatta tgtcaatgca gaacaagaat tatacacgac actgttaaaa gcctatcaaa
     1381 aattaaaaac cgaaaaagtc gaaaatatcc agaacctgat ttttgtctat gtaaaaaatt
     1441 ggtttatcga aaaaccaata gctgctaaag tatcaagtga aaaacgtttg aattatgaaa
     1501 gctccccaag cactattacg aaagactggt tagagtgaaa ctttactgac aaagtgattg
     1561 ttgcttcatg gagtaacatt aaaattaaaa attttttatt attctaatta tattacgaat
     1621 attacaaaat aatagctatt ttaatagaat ttataagtta aaatattatg ataaaaatca
     1681 caaacgtagc cttttacgtc ataagtgtta aaagaaataa ttaatttcaa atatagaaaa
     1741 gggaacgtat gtttgcatac tccttttttt tgagttataa taatacgcga agagagataa
     1801 tttcattatt taaacaagct aaaaatcttg atattacttc ttttcaggca atacgaatat
     1861 tattcgctta cattttttgt ataaatttaa ttgtctgcta tgcttgtgat gaattgattt
     1921 tcgttctgca cttttggatg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg
     1981 gtatgaactc ggaaggctgg taaccttcgt cagcgttcat acacggcatt aagatttggc
     2041 ccttaatgcg ttaacctttc agtgttacgg tattacccgc aatggattta gtataacaga
     2101 tattcaaaag tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg
     2161 atgaatctat gcctaagcac aatgttataa aaatgtccgt gtggctcttt gccgtatgac
     2221 gaacgctgaa attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg
     2281 aacgaaaatc aattctctat ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa
     2341 agaaattggg tggtttattt tatgtcatat acaggaattt tgagtctcaa agatatttgt
     2401 cactacggta aacggtgtac cgctacagag aaaatcacga agaaactatc aactggccaa
     2461 agtaaaaccg ttgtgcagtg taagaaatat ataatccaga aagacaaagt ctcagaggaa
     2521 atgatttatt acattgggaa acaaaagcaa ataattttaa aagatcctat ccccttaaaa
     2581 gagctatatc caacaatcaa acatgtttat gaccaaaacg gtgtactgat tggtcgaaga
     2641 aaaaatggag tcttgcgttg taccgcaaaa gggatggggc gtttgatcgg ctgaattgat
     2701 cattctcata acttgtattt tatcgttttt cgcttgcttg aaaatattgt cgagacttct
     2761 aaaggaactt agagtcacta gaaaaataag gagtagactt gatctatcaa caattccctt
     2821 cggggagtga cggggtaggt tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa
     2881 atgcgcttgt ttggatagtg gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca
     2941 agagggaatt tagaagttag acaagttaga atagcgtcta gattaatcat gagcagctac
     3001 taagggatct agactttgac gcaaactcat ggaataagga gtaggtcttc tcgtaatggg
     3061 gaggacgcac aagaaattgt gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt
     3121 gattattgga tcaatcgagc atttaccgct gtctactagg gtttctcgtt tcggacgcca
     3181 taaacgagtc gaaatttttt atgtattttt aagtaaacga aaaacgataa aatatgaaca
     3241 agtcctgaat aaagtgaaaa aattgatata aaacatagag aaaatgaaat ttacgtaatg
     3301 gaggaatgac aaatgaaaaa ttatgatccg aatatacgat tgggcacaca tactattaag
     3361 gtttctttcc aacgttggga ttacaaagga tttgtgacgt ttagaagagg cggaaattgt
     3421 aaaggattag atgttttagc attagacgag gatgatttat acgatcaaac attgactgat
     3481 aatccaattg gattcggctt gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg
     3541 acgcttatga atgataatgg agacgaatta tctgttgaag atacttggtc atatttgagt
     3601 gattatattg tcagctttga aatcattgaa tttgtagcgg ataaagaaga ataaaaactg
     3661 gaggaataaa ttttacagat aaaggcttag aaggagtcac gatgattaat aaaaaggtag
     3721 tattacctta ggcattgagt aaggaattt
//
LOCUS       DACEZN010000204         3724 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEZN010000204 DACEZN010000000
VERSION     DACEZN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.204"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(82..1029)
                     /gene="arcC"
                     /locus_tag="IXH60_002640"
     CDS             complement(82..1029)
                     /gene="arcC"
                     /locus_tag="IXH60_002640"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1991522.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(1144..2163)
                     /gene="argF"
                     /locus_tag="IXH60_002641"
     CDS             complement(1144..2163)
                     /gene="argF"
                     /locus_tag="IXH60_002641"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1991523.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(2254..3483)
                     /gene="arcA"
                     /locus_tag="IXH60_002642"
     CDS             complement(2254..3483)
                     /gene="arcA"
                     /locus_tag="IXH60_002642"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1991524.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         1007 a          776 c          645 g         1296 t
ORIGIN      
        1 aaaaatccag atggcctaaa ggagaagaaa taccacctgg atagctataa atcatttcgt
       61 aaatacgaaa gacttaacaa attaatcagc tacgataatc gttccttctt cagaagctaa
      121 aagattctcg atattttcta aagaagtgat gattgcctta gcgtttggtt ttgcttcaac
      181 aaatgcaata gctgcttcta ctttcggaag catgcttcct ggagcgaatt gcttttcatc
      241 aatgtattgt ttcatttctg aaacagtaac agtttctaat ttcttttgat ctggtttttg
      301 gtaattgaca tacacattgt ctactcctgt caatacgatc agcaaatccg catcgatgat
      361 ttcggctaat ttttctgaag caaaatcttt atcgattacg gcttctcgtc cttctaatcc
      421 agtagctgtt tctacaacag gaatcccacc gccaccaaca gagactgtga taactccttg
      481 atcgaccagc gtttcgatga cacgtgcttc tttaatcgag atgggtttag gggaagcgac
      541 tacttttctc catccacgtc cagcatcttc tttgaatgtc acagtggagt cagcattcat
      601 ttgttctttg gcttcttctt ccgtgtagaa tggaccgact ggtttacttg gatttttgaa
      661 tgaaggatca ttttcatcca caataacttg agtgactaaa gaaaccacat ctttatcgat
      721 ccctttttcc ttcagtactt cgcccatggc gttttgtaac cagtaaccga tagatccttc
      781 tgtcattgca acacatgtat ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg
      841 ctgctgaatc aataggttac cgacttgcgg accatttccg tgggagatga tcaattcatc
      901 tccttgttcg ataaatttca ctaaatactt tgctgtttcc attaacgctt cttgttgggc
      961 ttttgcactt gcatccgtag acaagatggc attgccccct aaagcgacaa ctacttttcg
     1021 tttggccatt tcattcgtcc tttcttcagt tctaccgaat tttctagata acaaaacgag
     1081 ccagcgtttt gtgattttcc ctcaatgctg actcgttttt tcgtgagatg aatgtggtta
     1141 tgcttaaacg cgagggataa acaagttacc taatgttgct gccatgatcg ctttgattga
     1201 gtgcatacga ttttctgctt ggtcaaattg acgagcatat ttgctgcgga agacttcatc
     1261 tgttacttcc atttcagtga tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt
     1321 tgtatcatgg aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt
     1381 ttctaccatt tccatattga tttggtaagg tttcaacaat ttgatacgtt cttcgaattt
     1441 atcttcttca cccattgata cccatacatc agaataaagg acgtctgctc cgtctacacc
     1501 tttatcaaca tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa agccttctgc
     1561 cattttgacg atttcttctt caggttgtaa ttcttttggt gcaacgatac gcatattcgc
     1621 tcctaaaatt gcacctgtga ctaataaaga gttagccata ttgtttcgac catctccgca
     1681 gtaagcaaca gtgatacctt ctactgttcc aaagttttct tggatagtta aaaagtcagc
     1741 gatcatttgt gttggatgcc attcatcagt caagccattc caaactggaa caccagagaa
     1801 ttctgctagt tcctctacca ttttttgact gaatccacgg aattcgattc cgtcaaacat
     1861 acgtcctagt actttagctg tatcttctgt ggattctttt ttacctaatt ggatatcatt
     1921 tgcacctaga tattcaggat gtgcacctag atcgatcgct gcagtagtaa atgcagaacg
     1981 agtacgtgta gatgtttttt caaataaaag cgcaatattt tttccttcta gatagtgatg
     2041 tgggatacca cgttttttca agtctttcaa gtgttctgaa aaatcgatca aatattgtaa
     2101 ttcttctttt gtaaagtctt tttctgctaa taagcttctt ccttggaata ctgattcttt
     2161 catattaaac attctccttt agattgtttt tatttaagat taaagaggaa atcccgacgg
     2221 cagctgacag taagactgcc gtcaggaaac aaattacttc aagtcttcac ggacaagtgg
     2281 ttggctcatg cagcgtggac caccacgacc tcgtgacaat tcacttgaat tgatttctaa
     2341 cactttgatg ccatagctac gaagcaattc gttcgataca taatttcggt tataagtgac
     2401 tacaacacct ggagcgattg ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc
     2461 aacgatctca tctccattac cagttgggat cagtacaaga tcatctaatc caagagcagc
     2521 tttcatcgtt tcgtgtaagt tatctgaatg agtaatcttg atatcatcac catctggttc
     2581 gattgtaaag acatccatct taccgtcttt actttgaatt gctggatgga tagtgaattt
     2641 atcgtgatct accattgtaa atacggtatc caaatgcatc attgcacggt tgttcggaat
     2701 tttgattgcc aacacttttt caaatcctga attttttgcg aataggtggc gtgctaattt
     2761 ttctaaagct ttcgcattag ttcgttgaga aatcccaaca gctactactt tatcgcttaa
     2821 gatcaattcg tctccgcctt cgatatgatc tggattttca cgatccaacc atacttcgat
     2881 tcctttattt gcaaatcttg gatgatgttt caaaatatat tctgtaaaca tcgattcacg
     2941 acgacgtgct tcaaaagtca tcttattgac tgtcatccca tttcccattg atgcggaagg
     3001 gtcgcgtgta aagtacaagt ttggcattgg gtccatgtaa aatggatagt catcgtctgc
     3061 tgacagatca tatagactgc tagaacgtac ttcgatatct tttgtacgaa ccccagccat
     3121 gattttatct accatttctt gcgtttccat tgatagaagg aactcatgaa gaccctctct
     3181 tacagcattc gatgcaatat gagattcatc caacatttta ttcaagaatt gttctttgac
     3241 attgcctgcg tcaatcgctt ctgccgctaa tttttccaag taaagagtct cgacgccttc
     3301 attttgcaat gtcttcgcaa agttgtcatg ttcttcttga gcaatcggaa gatatggaat
     3361 atcatcgaac aacagacgat ccataatatc tggtgtcaaa ttttccactt cttgtccagg
     3421 ccgtttcagt aaaactgtct ttaatttccc tatttcagag aaaacgtgaa taggtttatc
     3481 catgttgtgt tgcctccttc tttttgatta catgtatagg atatcctcct tttgtaatca
     3541 ctttcataat tttgtctcat tcaaaatgag acattcctca cattaatatt cgataattaa
     3601 ataattaaac gtttttctat ttttaagcgt aatttattca ttttttattc tgtatacact
     3661 ttcaaaaaaa tatttattca ttgaaaaaat ttttttgaat aaattaaaaa aataaattat
     3721 atta
//
LOCUS       DACEZN010000205         3706 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEZN010000205 DACEZN010000000
VERSION     DACEZN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.205"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            174..1478
                     /locus_tag="IXH60_002643"
     CDS             174..1478
                     /locus_tag="IXH60_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1991525.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1574..2443
                     /locus_tag="IXH60_002644"
     CDS             1574..2443
                     /locus_tag="IXH60_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1991526.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2555..2875)
                     /locus_tag="IXH60_002645"
     CDS             complement(2555..2875)
                     /locus_tag="IXH60_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1991527.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            3045..>3706
                     /locus_tag="IXH60_002646"
     CDS             3045..>3706
                     /locus_tag="IXH60_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1991528.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SIT"
BASE COUNT         1244 a          702 c          741 g         1019 t
ORIGIN      
        1 tatgaagatg aaaaattgaa aaaaagacaa gaattacttg aagaagtgta tacggaacgt
       61 tttggaacaa aagaagagag agaaacggtc cgtttttatt ctgtctccga agagaaaaat
      121 ctagatacga cattcattgc agatttgtat aaagaaaatg gggtggaact gaaatgatct
      181 caatcaccca tacaggtaat ttattcttag atacttgttt gtctattatg tatttttttc
      241 tcgtttctta tccgatttta ggcggatttg tatggtttat tggtgtctgg tgttatgtgt
      301 ttttatacaa acataagcaa aaagaatggg tagatgttcc cttgagtgtc gaaccgttca
      361 tcacgatcat ggtgccagcc cataatgaag aaattgtgat cgaagataca atcgagtatt
      421 tgatgacgaa attgaattat cacaactatg aggtattagt gacagacgat ggttcaacgg
      481 accaaacacc agaaatatta gctagactga tgaaaaaata cgctaatctt cgtgtggttc
      541 ggatcgaaaa aaataaagga aaagcccacg cgtttaatat cggacttgcc tttgcaaaag
      601 ggaaattgat tttaagcaat gatgcagaca cggtaccgga gccagatgca ctgattaggt
      661 atgtgaatta tttcattcgc cctggtgcaa gacatattgc tgcggtcact gcaaatatgg
      721 atgtccaaaa tcgtaccaaa ttgattgcaa aatctcaaac ggttgaattc tcaagtatcg
      781 tgggcatcat taaacgaaca cagtcagctg ttttcggtgg tctttatgcg tacagtggag
      841 caaatacgat gtatcgaaaa gaagccttga tcgatgtggg cggattcaga caagatcgag
      901 cgacagaaga catcagtatt gcttgggatc atcagttaaa tgactggtta tctgtttttg
      961 ctcctgagat tatctttttc atggaagtac ctgtgacgtt gaagatgcta tatcgacaca
     1021 gaaaacgttg ggcaaaaggt ggaacagaag tctggctgac gaatttcaaa aaagtgatgc
     1081 tccatccttt taaacatatt ggaagaacga tcatcttcat tgatcaaaca ttgagtattg
     1141 tctggtcaat attcttttgt atttccgtag ttctttttgc tggattaatc gggcattatg
     1201 tatatcaagg aaactacgaa cagatttata ttacttttac attcagtttt gtgttcatct
     1261 gctttgaaat ggtcgcagga tttttccagt tgctagcttc gctgattgta gatgatcgac
     1321 gaagaaaatt gaaatacctt cttttcgcac cactatatat gcttttgttc tggatagtga
     1381 acgcgataac gatcgtgacg actttcattc cagcagtcaa aacgatttta ggttatggaa
     1441 gcgggacatg gaaaagcccg gaacgtacaa aaaaataaaa gatgcaccgt tttttagaca
     1501 aacagcctgt tgtgtttatg gttcttccaa acatttttgc ttataataag aaattagtta
     1561 aaaagtagta aggatgggaa aagtgatgaa aaaaagaatg aagccgttaa tctggccaat
     1621 tgctgtttcc agtattttct tattgggaca gacagctttt gcggaaactt ctgaacctgc
     1681 tcaaacagaa ccttcaacaa ccataagctc ttctggtgct gaaaacggtg cctcggaaac
     1741 agaaatgact tccaatgtac cgacagataa ttcaggagaa acgatttcat cttcagaaga
     1801 tacatcaata aatagttcta cctcttctga ccaaatcgat catttgcaag acttggaaaa
     1861 taaaagaaaa gaactcgaag aagcattaga agctgcaaaa aacaaattga aagaagacca
     1921 agcagcaatc gatcaagatt tagctgatct taaaaataaa gtgggaaatg accgtgcaca
     1981 acttgagaaa aagcttgccg aagcgaaagc aaaagcggaa gctgtccaaa cagatcttga
     2041 cgggaagatc caacaagcaa aagaattcaa tcagcaatta aatcaggttg ctcaaacagc
     2101 ttctggaaag ctaagtcaaa ctcaagcgga tatcacagcg gccagtcaag caatccacgg
     2161 aaagatcagc gaagcacaaa cagttgctaa tgattttgaa gcaaaaaaag ctgacttagc
     2221 agctagtttc caacgaataa aagatatttt gcattcggca ggagaaaaat cagtgacctc
     2281 tggaagcacg acagaaacag ttcagcaaac gacagagcca tccaaatcaa caagtcagac
     2341 tttgccaaaa acaaatgaaa aaacagcttc tgtcagttat tccttagcag gggcgggtgt
     2401 gttagtcgca gcagcttacg gaatcatccg caaaaagaaa taaaaaagag aggatgggaa
     2461 ggtagagtac ctcgaagtta gactgaaaaa tctaattctt gagtgccact acatgtccca
     2521 tctctcttta gttattaaaa cagatgtccc ctacttatct cgtggagaca gctgttccac
     2581 tacatgtgac catcagcatg ccattgtcgc tgcctaatac ttcatagtcc atttttacgc
     2641 caatgatcgc atcagctcct aatgcttttg cacgttcggc catttcggca agtgcttctt
     2701 ctcttgcacc cattagttcg ttttcatagc tttttgagcg gccgccaaag atatttcgca
     2761 accccgcctc caagtcttta aatgcatgga tcccagtaat gacctcacca aaaacgatcc
     2821 ctttataagc tactacggct tttccctcta cactgttcgt tgttgttgta atcattcgat
     2881 cactcctctc ttttaattaa agtataacaa acttcaagaa gcactacaca tattttccca
     2941 aaattagaaa tcgccgattg taaaattaag ctagacaact aaaaaagtca tttgtgctac
     3001 actcaaaata gttccgtcga aagaattata aggagtgagt acaaatgacc tatacccatc
     3061 ttacaccaac tgaactcgta atgatagaag cttatttcaa tcaatcacac tctgtttcaa
     3121 aagtagccca tctggtacaa cgttctcgac aaaccattta taatgtctat cgttttctta
     3181 aatctggtgg aagcgcaata agctactatg aacaatataa gaaaaacaaa cgtaactgcg
     3241 gaagacgtcc gattgtgtta ccagatgggc aacaagaata cattcaaaaa aaggtggctc
     3301 aagggtggac tccagatgtc cttattggtc gagcagagtt tcctattgct tgttcggtac
     3361 gtacactata tcggatgttt aaagataaaa cctttgaccc tcacgattta ccaatgaaag
     3421 ggaaaagaaa accaaacgga cataaagaaa aaagaggaaa acaagctttt cgccgttcga
     3481 ttcaggatag aacaactgat tatatacagt tcaatcatga gtttggtcac ctagaaggtg
     3541 acaccattgt cggtggaaaa cataaaagtg cggtgatcac gttagttgag cgattatcta
     3601 aagtgatcat caccttgaaa cccgctggta ggcaagctat agatattgag aatagactga
     3661 atcaatggtt tcaatctgta ccgaaacact tgtttaaatc catcac
//
LOCUS       DACEZN010000206         3663 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEZN010000206 DACEZN010000000
VERSION     DACEZN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.206"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(298..660)
                     /locus_tag="IXH60_002647"
     CDS             complement(298..660)
                     /locus_tag="IXH60_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991529.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(664..972)
                     /locus_tag="IXH60_002648"
     CDS             complement(664..972)
                     /locus_tag="IXH60_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991530.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(981..1400)
                     /locus_tag="IXH60_002649"
     CDS             complement(981..1400)
                     /locus_tag="IXH60_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991531.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(1393..1626)
                     /locus_tag="IXH60_002650"
     CDS             complement(1393..1626)
                     /locus_tag="IXH60_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991532.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(1629..2597)
                     /locus_tag="IXH60_002651"
     CDS             complement(1629..2597)
                     /locus_tag="IXH60_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1991533.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(3044..>3663)
                     /locus_tag="IXH60_002652"
     CDS             complement(3044..>3663)
                     /locus_tag="IXH60_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1991534.1"
                     /translation="SDELRYISRQREENERDKALMASERAKIEEEKKNMLNVKKEMAV
                     IFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQN
                     TELNEYVATLEEERHLSGRKQLADLQVENKKLKEENRSLRQRLERLKNAFQKSITRLS
                     IRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRKLERSEDEIEW"
BASE COUNT         1054 a          835 c          514 g         1260 t
ORIGIN      
        1 atcagttaac tcacgattat tatgagaaat acttgtattc ttcgttcctt ttttaaatga
       61 aatactaaac cccaagttaa aaccctcctt cgcgctgtcg ctaataaaaa gattgtaaac
      121 ctttctgtta ctctttgtca aagagtaaaa attttcctaa ttgtgacatg gctgtcacaa
      181 ttccaaatgc tctccgaggg tattcgctgt cacaagtgac aacgaaaaaa ggagccgaaa
      241 gcgtgtcacg ctctcaactc cttttacgct ttattttgct tcacgcaaaa taatcgctta
      301 tcgtaaattc acgtcattaa atattgacgt aaatttactc tcactcatct gacttttctt
      361 attcggtatc actccgaata agccgtcaga ttcatcggga taatctcgtt cgcttggcgc
      421 tgatttttca tcagcaagcc actcattatc cactgatttt tcgtgttgct gatcgtcaga
      481 aatcgacgat agccaccgct caaaatcagc aaaagtttca aacccaaaac gcttgcgaag
      541 agccttgccc attgcagcat tttctttctc aatcaagcgt tttttctgtt ggcgaagttt
      601 cgcttgttct ttttttaatt tttcttcttg cgctttaata tctgctaaag tcattcccat
      661 aaactacatc tccttttctt tggaattaat aacggaaatt tggtttaact gcataacatg
      721 actcattaat ttcaaccaaa attctttttc tgattcatca gttgtttgcg ctaaatgctt
      781 atgaacaaat tcattagtca aaactgaaga ttttgctaga tcagcattga taaccgagtt
      841 gagaacatcc atcattcgtt caagcatttc actataatta tcttcatccg gcaaattcaa
      901 ataatcagct aaccgttcat acatcaattc aattctatcg actgtttctt ctctcaaatc
      961 aattttatcc atgtcacacc tcacttccag aattgccacc attttcgatt ttttaattct
     1021 tgattttcta gtaacaactg atcgtttttt tgttgctgaa gcaaagtcaa tttttgttgg
     1081 tcacgaatca tttccgttaa ttcagaaata tgcctatctt tttccgctaa ttgttcacgc
     1141 aaaaattcat tttctttttc taacgggaaa tcattgcctg catcattgac cggtaaatca
     1201 ccagtcaatt gaccaccttt tgaccgataa attgaccgca caacaccaat caattccttg
     1261 tctaacatga taaccccatt atctttatac cgagcaaggt cataacctgc attgaccagt
     1321 tgattgcgca ctgtattttt agatataccc aactcatcag caaggttttt tatggtcatt
     1381 ttttctggca aatcagtcat tatcttctcc gttactttca gctaccccat aaacatattt
     1441 aacagcatgc ttagggataa tgattgggga atcaatttta ttatttttca aaatcaactc
     1501 atactgcgtt acatcaagta acttaccgga catttttttg ctatccaaaa aaacaacgac
     1561 aacgttatca cctgttaaat tcaccaacca atttttctta ccaaaatgct tttgtttttc
     1621 tgccattctt attcctcctc cgttacatct aacccttgaa gccaagtgtc taattctttt
     1681 ttcaaaacat ctgctgattc cgaaaatgtt tctcgtaaat gattcgtcaa ctcatcattt
     1741 tgactttctc ttttcaaaat atctctcaat ttgtatttca ttctacgctc taaaaacccc
     1801 cacatttcag ggttagcaat cagtccaaaa atcatcacct tatcatttgt ttttacatca
     1861 agtggtagaa aatgagggct agcaaaacta tttaaacttt catgaaccat tgcatgccaa
     1921 tctgttgctt tcccacgcct taactgcaat tccagtcgcc accaagtttc tatttctggt
     1981 ggcacaattt tacgcttggt ttcctgttcc aattttttgt tgtatagccg aatctggcgt
     2041 tcgctggcac gactgcccca ataagctgtt tccagcttcc cagaccgccc ataaattggc
     2101 ttgaaagaaa caggatcaac cactcgatat tgtgtaataa actcatcagg cacatcgata
     2161 atatcgcaag caatatccgc acgtgagaaa tgtggttcta gaaataaatc atgaatgaaa
     2221 tttttcattg aactagctaa aaaatgacca atcttattcg ggttaaaatc aatccgccct
     2281 ttgccatctt gccatttcaa aatctcaata taagcaatat tttcttcgta ttggtctaat
     2341 aattgccagc cgttattccc aaccgctttt aaatagccat tgccttcatt cacccgcatt
     2401 aattgctcaa aatctaaaat caacacatca ttttgagtgt gataataaat attttccttc
     2461 agttttccaa cgatagtaat tcggtcaatt gaccactgca tttttaaggg ggtgtacata
     2521 ttttgtacat ttaaccgcct gttagatagg gctgttaaat tttcccactc atttacgatt
     2581 atgccatttt tactcaaatt ttgttcctcc tcaaagaaaa agagacttct ggaaagaatt
     2641 ttcaaaagtt cacgtaactc ttaaaaattc attaccaaaa aatctctagt cttgaagaaa
     2701 tctaaaaata ttcgtataat gattaagaag ttacggagct agtgcgatta gtttccgtaa
     2761 aggtcgagaa cgttggtagc gttgctcgac tattttttta ctcttttttc agttcatttg
     2821 tttgctaact caataatagc acttctcttt agctatgcaa atatccaaaa aataaaaccg
     2881 caccctttga aaagggcgcc cccaaacttc gctaacgcta ggttggccaa aaaatggact
     2941 ggccattttt tagcaaatgt cagcttaata ggctagtggt ttagccgtca actccttatc
     3001 ctcggcacaa accgcctccg gtaccgttga ctgcacatcg taactaccac tcgatctcat
     3061 cttcagaacg ctctaattta cgctgtgaga cgttttgacg ataaaataag gttttctcct
     3121 gctctaacac ttctaattcc tcagaaagcc ccatatcctt ttctgcaaga ccaaaacgga
     3181 tagacaaacg agtgatcgat ttttgaaatg cgttttttaa tcgctctaaa cgctggcgta
     3241 aagagcgatt ttcttctttc agctttttat tttccacttg taaatctgct aattgttttc
     3301 taccagacaa atgacgctct tcttccaatg tagctacata ctcatttaat tctgtatttt
     3361 gctcaagcat ctgtaaatat tgctcactca tgttatcagc atttctcttg gcatttgttg
     3421 ctctcgtagc tcgttgatac aaatcaacaa aatcattccc agataaatga tacgaaccat
     3481 caggaagcct cttgaaactt tctaaaggaa ccatatcttt ttggaaaatg accgccatct
     3541 ctttttttac attcagcata tttttcttct cttcttcaat cttcgctctc tcagaagcca
     3601 tcagagcctt atcacgctcg ttttcttctc tttgcctaga aatatacctc aactcatcag
     3661 aaa
//
LOCUS       DACEZN010000207         3571 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEZN010000207 DACEZN010000000
VERSION     DACEZN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.207"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            214..996
                     /gene="rpsB"
                     /locus_tag="IXH60_002653"
     CDS             214..996
                     /gene="rpsB"
                     /locus_tag="IXH60_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1991535.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1095..1976
                     /locus_tag="IXH60_002654"
     CDS             1095..1976
                     /locus_tag="IXH60_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1991536.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2112..2834
                     /locus_tag="IXH60_002655"
     CDS             2112..2834
                     /locus_tag="IXH60_002655"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1991537.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2837..3394
                     /gene="frr"
                     /locus_tag="IXH60_002656"
     CDS             2837..3394
                     /gene="frr"
                     /locus_tag="IXH60_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1991538.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1315 a          610 c          782 g          864 t
ORIGIN      
        1 ttctttttat ctttcaggta aactattgaa tgttgagcag aaaagtatat ttgctcttgc
       61 gatacggatc ttttgtctgt ataatagctg agggtgcttt atgcatcagc aaatccacat
      121 tcttattgga ttgtcggtag gtgcttgaaa aagtttgccg gcaaggtaga agataagaag
      181 aggaaaaata aaaccaaatc ggaggaaaca aacatggcag taatttctat gaaacaatta
      241 ctagaagccg gcgtacactt tggtcaccaa actcgtcgct ggaatccaaa aatgaagaaa
      301 tatatcttca cagaaagaaa cggaatctac atcattgact tgcaaaaaac agttaaatta
      361 gtagatgcag cttatgatta catgaagagc gttgctgaag aaggcggcgt agcattattc
      421 gtaggtacaa aaaaacaagc acaagaagca atcaaagaag aagcaactcg tgcaggacaa
      481 tacttcgtta accatcgttg gttaggcgga acattgacaa actgggatac tatccaaaaa
      541 cgtatcagcc gtttgaaaca aatcaatgca atggaagaag atggaacttt tgaagttcta
      601 cctaaaaaag aagttgctgg tttgaacaaa gaacgtgaac gtcttgaaaa attcttaggc
      661 ggtatcgctg atatgccaag aatcccagat gtaatgtaca tcgttgaccc acgtaaagaa
      721 cgtattgctg ttcaagaagc acacaaatta aatatcccaa tcgtagctat ggttgataca
      781 aactgcgatc cagatgaaat cgacgtagta atcccttcaa acgacgatgc aattcgtgcc
      841 gttaaattga ttacttcaaa aatggctgat gctttcatcg aaggaaacca aggtgaagat
      901 caagtagttg aagaagattt cacagaagaa aacacagcaa catcaatcga agaaatcgta
      961 gatgttgttg aaggcgacaa ttcttcagca gaataatttt gaatgatgga gctgtttcaa
     1021 aggctgggcg acaagcctca ctctcctttg aagcagcttt ttttaaaaag aaaaacacac
     1081 aggaggagtt aaacatggca gacgttacag ctaaaatggt aaaagaacta cgcgaaatga
     1141 ctggcgtagg aatgatggat gcgaaaaaag cattagttga agtagaaggc gacatggaaa
     1201 aagcagttga tcttttacgt gaaaaaggaa tggcaaaggc tgcgaagaaa aatgaccgca
     1261 ttgcagctga aggattggct tcagttgctg tgaaaggtaa cactgcagca atcgttgaag
     1321 taaactcaga aacagacttt gtttctaaaa acgaaatgtt ccaagattta gtaaaagaaa
     1381 ttgcagaact tgttgctgaa aacaaaccag cagacatgga agctgcaatg aaaatcaaaa
     1441 cagataaagg tacaattgaa tctgacttga tcgaagcaac tcaagttatc ggagaaaaaa
     1501 tcagcttccg tcgctttgaa gttgtagaaa aagacgacaa tgctgcattt ggtggatact
     1561 tgcatatggg cggacgtatt gccgtattga ctgttctaga tggaacaact gacgaaacag
     1621 ttgcaagaga cgtagctatg cacgtagcag ctatcaatcc tcgttatgta aacgaatcac
     1681 aaattccaga agctgaatta gaacatgaaa aaactgttct tacagaacaa gcattgaacg
     1741 aaggcaaacc agcgaatatc gttgaaaaaa tggtagaagg acgcttgaag aaattcaaag
     1801 cagaaatcgc tttggtggac caaccattcg ttaaagatcc tgatatgact gttgaaaaat
     1861 acgttgcatc aaaaggcgca actgttaaaa cattcgtacg tttcgaagtt ggcgaaggta
     1921 tcgaaaaacg cgaagataac tttgtagaag aagttatgag ccaagtgaaa aaataattct
     1981 gttgaataac agacgggaac gcacggtttg tgcgctccct ttttttaagc aaaacaagaa
     2041 aaaccagtct tagagtgtcg aaatcatcta agtatggtaa aatggcaata gaaaatattg
     2101 gaggaatgaa catggtcaca ccgaaatatc agcgtgtagt cttaaaattg agtggagaag
     2161 ctttagctgg ggacgcagga tttggaatca aaccaccagt aatcaaagaa atcatccaag
     2221 aaatcaaaga agttcacgag ctgggcgttg aaatggcaat cgttgtgggc ggcggaaata
     2281 tctggcgcgg acaaatcgga gcacaaatgg gcatggaacg tgctcaagct gattacatgg
     2341 gtatgttagc tacagttatg aatgccttag ctttacagga tacgttggaa aacgtgggtg
     2401 taccgacacg cgtgcaaact tcgattgaaa tgcgtcaaat cgcagagcca tatattcgcc
     2461 gtaaagcaga acgtcattta gaaaaaggac ggatcgttat ctttgcaggc ggtaccggca
     2521 acccgtactt ctcgacagac acaacagcag cattacgcgc ggcagaaatc ggcgcagatg
     2581 ttatcctgat ggctaaaaac aatgtagacg gtgtttattc tgctgatccg aaagtagatg
     2641 caaatgcagt aaaatttgaa gaactgactc atctggaagt tattgccaaa ggattacaag
     2701 tcatggattc aactgcaagt tcactaagca tggataatga tattccactg cttgtcttca
     2761 atttaaatga acacggaaat attcgccgtg caatcttagg cgaaaatatc ggaacaactg
     2821 taagggggaa ataaaaatgg cagatgcaat tatgacagaa gcaaaagaaa aaatgaaaaa
     2881 agcagcacaa aatcttcaac gtgaacttgg gcaaatccgc gcaggccgtg cgaacgctag
     2941 ccttttggac cgtattactg tgaactacta cggtgcgcca acaccattga accaaatggc
     3001 atcaatccaa attccagaag cacgtgtttt aatgatcaca ccttttgaca agagcattct
     3061 tcaagatgtt gaaaaagcta ttatggcaag tgatatcgga atcagtccaa ccaatgatgg
     3121 aaatgtcatt cgcttagtta tcccgcaact gacagaagaa cgcagaaaag aattagctaa
     3181 agatgtgaaa aaagaagcag aaaatgcaaa aatcgcgatt cgtaacatcc gtcgtgatgc
     3241 gatcgatgaa tataaaaaac aacaaaaaaa tggtgatatt accgaagatg atttgcgcgg
     3301 attagaaaaa gacgtacaaa cattgacaga taacagcatc aaagaagtag ataagatcgc
     3361 tgctgataaa gaaaaagaat tattggaagt ttaataaaat cataaaaaca gaggggcgga
     3421 tcattccgct tcctctgttt ttatttatat ttcttttatt taaaaattaa aaaaagaaaa
     3481 aattattcag tttttaaata aaaaaacaat gatttcatag gaaaaacagt tagatattgc
     3541 tataatactt aagatagtgg gaatctttta a
//
LOCUS       DACEZN010000208         3499 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEZN010000208 DACEZN010000000
VERSION     DACEZN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.208"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(92..3319)
                     /gene="fss3"
                     /locus_tag="IXH60_002657"
     CDS             complement(92..3319)
                     /gene="fss3"
                     /locus_tag="IXH60_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1991539.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVNCTVERHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT          906 a          737 c          630 g         1226 t
ORIGIN      
        1 ttagttttgg atacaaaaat agcgcgacta cttttttagg aggcggtcgc gcacgttggt
       61 tcttaggatt tacttgtatc atctcatcac cttagttccg gcgattccag aaataatagc
      121 ccgccgtcgc aaaaatcaaa gtaaaaccaa tgaacagtaa aacattggaa tttttctccc
      181 cggtttgagg gaaggtcttc actgtgggac tagattcttg agagccactt ggaacagttg
      241 gtgtttccgg ttgtttcggc gtacttagta tggttggaac ttcttttggt gttaaggttt
      301 gagatttttc tttcaaatct tcattgtgtt ttccattgat attcccatct ttatcgtaat
      361 tgatttccgc aaaagtaaac ttggttcctt ctggatattt tccggtatct acttttttcg
      421 caagcacagt tttggtgaat tctttatcat ttacttcata atcaatcttg ccagatttcc
      481 aaatttcttt gtttgtacca tccggtaata aagcatacag aattgtttcg aaagcttctt
      541 ttgagccatc cagtacatca tgagtaatcg acacatcatc aaacatatcc accacatcac
      601 catgagtaaa ggtctgagaa ccgttttcta ggtgggcctt tgtttggata gaaacatgac
      661 gttccaccgt acaattaaca gtttgggctt gattattcaa gctaacatcc ttcgctactg
      721 gcttatcgcc tgcttcaaaa gcttccttat tttcatacac atagttgaat aagacaatag
      781 ttttatttaa aacttgttcg gcggttaatt tatgctgaat tttccaagtt cctgttttct
      841 ctttgttcgc caacgttgcg cttgtttctg caatcaccac tggtttggct ttttcatctt
      901 tttcttgggc agcttttgtc gcatccacat caatggcttg cgctaccaca taccagtctt
      961 ctttgatttc atgtagattg taactcaatt tatcaactag ctcggtagaa tccagagaag
     1021 ttaattcttt gtttccgtct ttccaagtcg caagagaagt taaactatct tcttcctcag
     1081 gcaaatcata gagcattaaa cggttcaaac taacagaaaa tgctttgttc gtcagcttct
     1141 cgtaaggaac ggtcaccgtt ttaattggtt gtttttggtc ttgttcggta atggtaaaga
     1201 catactcact cttcacaaag tcttctttgt tttccttaaa agtagaacgg atttctaatg
     1261 gcgtaatctt ttgaaaacct tctggggctt ctacttcttc aagtaaataa tccccataag
     1321 gaagattttc aaacttgcca taaccatcaa accctaattg ctcgttataa gctgtggtcg
     1381 cttcatcttc ggcacctgtg atttcattgg tcccttccaa tggcgacact ttaaaggtca
     1441 gatcattgaa gccagtttcg gtagtgcccg cagctgatcc agcaaattta aagaaatcaa
     1501 agccaaagcg aatcacttgt tcttttgccg taacatttcg ggtaatcacg gcattttttt
     1561 cgctatcatc cacctttttg atggatacag gatactttgt ttcatcaagg ctatatcctt
     1621 cgggcgcttt tgtttcttgc caataatact cgttgatagc taaatgttta acggcaactt
     1681 ggttcttttc gtctaaagtc aaagtgaccg tttcatcaga acctttcgtt cccttcgcta
     1741 actctggttt aaaagcctca ctccatttaa cagcttttcc atccttagca gtaaacaagg
     1801 tgtattcagc accctcaaag actgccttac cttgtgcttt atccccagta tctttgtctt
     1861 ctttcgtcaa agtggtttcg ccagtgactt cttgattttg cccttttacg ttactggtaa
     1921 caagggctac ggtttgattg gcatacttca attcgacttt tacaggtttg aacgtattca
     1981 cgaacccatg actagcttta gtttcggtca cataataggt tcccaattcc aacgcattag
     2041 caatctcttt tggtgtttcc gcatgaccgt tttcatccgt ggtcatttct tgaacgattt
     2101 caccggttgg actgtctttg cgaatggcaa acgtattacc agctaaggaa taattgtcat
     2161 tccaaagatc actccctgtt tcaactccgc ttttatccag aatgatttgc cctttttccc
     2221 gtgcgttttt tgaagtcacg taaatggttt cgcctgcttt aatggtggta gtcatggggg
     2281 tggtatcaat cgtataaggc gccggtactg atttttcggt aatggttact tttgtaccat
     2341 gaggaatctc atccaatgta gcaatgcctt ctttgtcagt agtcacgtct tttgcaggta
     2401 aagtttttcc aaagtctaaa tggaaaaccg ttcctggtac aacatcaccc gattctttat
     2461 cgactttttt gatctttaaa gaaccctccg tttccacatc aattttgatt gtgtaggtat
     2521 taggtttatc aatcgccccg gccattaaag tctgttggcc agccttttta taggcgacag
     2581 gtgttccggt accagcagat ttcttaagcg tcagcacacc actttctttt gaattggcat
     2641 ttggggtgat tatcaactga ttaccattca cacgataatc aatgtttgcg gcattttcca
     2701 caacctcatc gaactctgac aaattcagat tattcgtatc cgtcacagta gtcgattgcc
     2761 ctaatactgt tttagccgta ctattatgga aggttggttt cttctgataa tcgtcaatgg
     2821 cttgatttat ttttgcctca atggctgcga tatttacggc actaccatct gatcgtttta
     2881 ttgagtgaag ggtataccca ttaacttctt gccagatcat cttctgtgcc acaatatgag
     2941 tatctgcatc tgtacctact tttttccata aaacggaaac taatttggct ttatcggaca
     3001 tatctggtaa cgggtttttc tcatagccag gagtgaactc attgtaaatc gggactcctg
     3061 gttcaataca gaaaatatct tgtttaaagc cgttgtacac tgcataatgc ggaacagtga
     3121 cttctgacat tggacgacca tcactaaaag ttccttcaac agcatattga tgtgctaaat
     3181 ctagttctac ggttaccgtt tgtggatggg taatttcatc agcaaaagta atcgtaggag
     3241 caattgctcc aacaattgct tgaacagcca aactcaaaat agttaataca cataatttct
     3301 ttttttgttt tttatacatg aaatgttctc ctttcaaatt aagcacaaaa aaataagatg
     3361 ctcgcttgaa catctttgtt tataaagtct ttttaaaatt tttattgact gaatagaatg
     3421 gatttgagaa tgaatgagcc taaaaattta atctaaaata cgaaatatac tgcctttaaa
     3481 ttagaacaca ttttagaac
//
LOCUS       DACEZN010000209         3498 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEZN010000209 DACEZN010000000
VERSION     DACEZN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.209"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(324..893)
                     /locus_tag="IXH60_002658"
     CDS             complement(324..893)
                     /locus_tag="IXH60_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1991540.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="IXH60_002659"
     CDS             complement(967..2256)
                     /locus_tag="IXH60_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991541.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="IXH60_002660"
     CDS             2610..3458
                     /locus_tag="IXH60_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1991542.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
BASE COUNT         1033 a          699 c          617 g         1149 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcatt
//
LOCUS       DACEZN010000210         3449 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEZN010000210 DACEZN010000000
VERSION     DACEZN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.210"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            83..970
                     /locus_tag="IXH60_002661"
     CDS             83..970
                     /locus_tag="IXH60_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1991543.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IXH60_002662"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IXH60_002662"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1991544.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IXH60_002663"
     CDS             2272..2718
                     /locus_tag="IXH60_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1991545.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..>3449
                     /locus_tag="IXH60_002664"
     CDS             2991..>3449
                     /locus_tag="IXH60_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1991546.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIV"
BASE COUNT         1179 a          548 c          735 g          987 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgtt
//
LOCUS       DACEZN010000211         3439 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEZN010000211 DACEZN010000000
VERSION     DACEZN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.211"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IXH60_002665"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IXH60_002665"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1991547.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IXH60_002666"
     CDS             complement(1098..2201)
                     /locus_tag="IXH60_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1991548.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IXH60_002667"
     CDS             complement(2291..3226)
                     /locus_tag="IXH60_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1991549.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
BASE COUNT          966 a          708 c          610 g         1155 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctata
//
LOCUS       DACEZN010000212         3433 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEZN010000212 DACEZN010000000
VERSION     DACEZN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.212"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IXH60_002668"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IXH60_002668"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1991550.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="IXH60_002669"
     CDS             2714..2938
                     /locus_tag="IXH60_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991551.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2950..>3433
                     /locus_tag="IXH60_002670"
     CDS             2950..>3433
                     /locus_tag="IXH60_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1991552.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFSPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPIGEVALEIQHAFFNSFEEMVEH
                     VDDIICYPDCNDMSDVAYYLIEETGAFGEVPTHLQNYIDYEAYGRDLELEGNYLVTNR
                     G"
BASE COUNT         1263 a          523 c          740 g          907 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 ccaatcttta gtcttattgg caattgtggt cattggttta tttttaggct tcaaacgtga
     2821 gttttccaag ctgattggtt ttttggtgat tgcgctgatt gcggtaggac tggtcttcaa
     2881 cgcttctggt gtgaaagatg tcttactcaa cttgtttaat cggattgtcg gtgcgtaagg
     2941 gggaccatta tggagcagat gcgtgtatat attgcgaacc ttggaaaata taacgaaggt
     3001 gaattggtgg gggcttggtt tagcccacct gtcgattttg acgaagtgaa agaacggatt
     3061 ggtttaaatg acgaatacga agagtatgcg atccatgatt atgagttgcc ttttgagatt
     3121 gatgaatata caccaattga ggaaatcaat cggttgtgtg gtttagcaga ggaactagaa
     3181 ggaacaccga ttggtgaagt tgctttggaa atccaacatg ctttcttcaa ctcttttgag
     3241 gaaatggtgg agcatgtgga tgatattatc tgttatcccg attgtaacga tatgagtgat
     3301 gtggcgtatt atttgattga agaaacaggt gcttttggag aggtgcctac acacttgcaa
     3361 aactatattg actatgaagc atatggacgt gacttggaac tagaaggaaa ttatcttgtg
     3421 acaaatagag gaa
//
LOCUS       DACEZN010000213         3422 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEZN010000213 DACEZN010000000
VERSION     DACEZN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.213"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..154
                     /locus_tag="IXH60_002671"
     CDS             <1..154
                     /locus_tag="IXH60_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAQ1991553.1"
                     /translation="YIENELTDESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYS
                     YARQYV"
     gene            284..742
                     /locus_tag="IXH60_002672"
                     /pseudo
     CDS             284..742
                     /locus_tag="IXH60_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
     gene            779..1414
                     /locus_tag="IXH60_002673"
     CDS             779..1414
                     /locus_tag="IXH60_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1991554.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            1527..2162
                     /locus_tag="IXH60_002674"
     CDS             1527..2162
                     /locus_tag="IXH60_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991555.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            2461..>3422
                     /locus_tag="IXH60_002675"
     CDS             2461..>3422
                     /locus_tag="IXH60_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1991556.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT         1380 a          498 c          570 g          974 t
ORIGIN      
        1 ctatattgag aacgaactga ctgatgaatc tcagtttaat ctcgaaaaat ttatggagta
       61 ttattctatt cagaataagc tcagatacac ttgttatgct gtctgccaat gctattttaa
      121 gaaaaaatat tcttacgcaa ggcaatatgt atgaatacat caagaattat taaaaaaacc
      181 gatttcagca aaacattaaa aaagcccgtg ctgcaacacg ggctctttcc tcatatatga
      241 gcttctacaa aaaaaatcat atcacagaaa tgaggaagaa aaaatggaaa aagagatttt
      301 aataaaatct ccaggaaaaa ccttgattaa agtaactgat gattctattt caattattag
      361 aaaaggattt ataaatttag ttaatcaagg tattaaagga gaaaaaacta tcccctttaa
      421 aaatatatca gccgtacaat taaaaaaacc aggtatgagt aatggttata tacaatttac
      481 cttgctaggc ggaaacgaaa gtcgcggagg tatattagcg gccacgaaag atgaaaacac
      541 cgttatgttt acaaaaaaat attggaatga aatggaaaat ttaaaaaaat atatagagaa
      601 gcaacaatct atattagata ataattccaa agaaacacaa atttcaagtg cagatgaaat
      661 taaaaagtat aaagagttgt tagatgaagg gattattaat caagaagaat tcgattttaa
      721 gaaaaaagaa ttgcttggtt tacaatattc aatatcaaaa gagaaagagg atctattaat
      781 ggacggtata tttggtttta ctggtattgt tttatttatt ataggtttaa tcatgttaat
      841 tgtaagattt atcaaaaaaa caaataagaa aacacctatg attctcctta ttatcgggat
      901 tatttttact accgtaggtt tctcgctttc ctcatcaaac gaggcaaatg acaataacaa
      961 acaggaaagc tcatcatctg atactcaaac tactacatct aacagcagca ataaaagcct
     1021 aactcaattt gaagaatttt tagagacaaa taatcatgac tggggtgctt tcttagatag
     1081 ttattattct attactccag ctactgagca aaatacagct tttactaaat atattagtgg
     1141 taaaacatat acttttgaag gaactgttat cgaatcaatg actactagaa tagccattat
     1201 agcagacaaa gagtacgatg ataaaagttg gaatgatatt tctgcaacac ctaaagtttc
     1261 atatgtaatt tttgcgaagg acttaaacaa tgctgatacc tttacaaagg gcgataaagt
     1321 aacttttaca ggagaaatta gttcaagagg atcgaacata gaaaaatcct atgctcaatg
     1381 ggatatgatt aatagtaaag taagtaaaaa ataaatgaac cacgccccac taagagttag
     1441 attgtgcaca ataataaaac ctatacaaaa gagtgccatc aggagtcaca atgcttgtaa
     1501 aggaaatgac aaaataggag gtaactatgg ataatagcaa tgaaaaacta accaaaaaag
     1561 aatatcttga attaatagtt gaaggcggta ttggagcaat tccttatatt ggtggcgttg
     1621 tgcagtcact ttattttggg tctaaaaacg agaagcgttt caaacgcatc gagaaattct
     1681 acaaaaattt aaacgaatca ttagaaaaca ttcgcgatca aattccagaa gatgttttca
     1741 acttagagaa caaggaccaa ctcattggaa tattcgaagg gataaatgac gaggtagaaa
     1801 aatcaaaggc acaaaataaa atagatatgt ataaaaatct ttataagaat tgtttacttc
     1861 gaattaacag cgcttcatgg gataacgagg agtattatct tcttgtactg aatcaattaa
     1921 catcgattga gattcaattg ttagcatgct taatgagtcg tggaaacgaa aatttcactg
     1981 gaaatatcag catgtctggc tattcacaag agctaataga tggtagctta aatagattat
     2041 ctgattttgg tctgttagaa aaaacgatta gctccatagt attaggtgat gtcggcaaac
     2101 aaaatatggg ctacaaaata tctcatttag gaattcaatt tattaactca actcttactt
     2161 aagttttctt tctaatatga catctagacc tatcagcaat gcaacaataa aaaaccagac
     2221 ataccaataa tcaacaaata atctaaactc tggaagttca gttattcttt taaaaatcac
     2281 tttaatcata ataaacacct ctgattggat gatctaatac acatccaatt ataccattaa
     2341 aacgacaaat agcacatcct tgcgccggca agcaaaaagg atgtgcaaaa aataataaaa
     2401 cccaaacaat gggcttcttt atagttccta ttgtatcaga gaaagagagc tgattcaatt
     2461 atggcaagat tagtcaaacg tggaaatagt tggcaatacg aaatttcata caaaaaagat
     2521 gacggaaaat acacgaagat aagaaaatca ggatttaaga caaaaggaga agcaaaagat
     2581 gccgccaacg aattagaata taacttgaac aaaggcctta aaggggatcg caaaaatcta
     2641 ttattatcag attactttga ggattggatg caactttata aagaaggaac agtatctcct
     2701 atcacttata gaaaatacga agatacgtta atgaacataa agaaatatat gccagcggta
     2761 ttgatttctg atttagatag agtgggatat caacgctttt taaataagta tgctaaagac
     2821 catgtaaaat ctaccgttat taagtttaat aaccatatta gagcgtcgtt gaaagatgcc
     2881 gtagaagaag gattaattcc gtttgatcca actagaaaag cagtaatcaa aggaaaagat
     2941 tcattgaagc caaaagaaga taaatattta gattatgatc aatttaaatc tttaatgaaa
     3001 ctcgtagaag aaaacctttc tgcgcagtac tcttctccta tgctcgtgtt agttgctggt
     3061 gccactggaa tgcgatttgc tgaacttcta ggattaacat gggaagatat cgatttcgaa
     3121 gatcaaatca tcactattaa taaaacatgg aattataaat taaatgaatg gggaaaaaca
     3181 aaaaacgaaa cttcaaatag gaaaatttcc attgataaac atacgattga tctcttaaaa
     3241 aagtttaaaa tcaatcaaaa agaattattc gagaattttg aaatcaaaaa ccctcataat
     3301 tttgtttttt tcaacttaaa aaatggatta gtttcatcaa acgccgtcag caaatatttg
     3361 cgcaaaaaat taaaagaatt agggattgaa aagcaattta ctttgcatgg actaaggcat
     3421 ac
//
LOCUS       DACEZN010000214         3376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEZN010000214 DACEZN010000000
VERSION     DACEZN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.214"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..550
                     /locus_tag="IXH60_002676"
     CDS             <1..550
                     /locus_tag="IXH60_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAQ1991557.1"
                     /translation="LELLDGYNSDLISEYKNEIETCVNEIRKLNWDIVSVKKEIQDKA
                     KVELEIEDLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDK
                     IDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNY
                     NEVSKRYQRGIKNLVKSYLKMN"
     gene            553..1449
                     /locus_tag="IXH60_002677"
     CDS             553..1449
                     /locus_tag="IXH60_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1991558.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1499..1888
                     /locus_tag="IXH60_002678"
     CDS             1499..1888
                     /locus_tag="IXH60_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991559.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1875..>3376
                     /locus_tag="IXH60_002679"
     CDS             1875..>3376
                     /locus_tag="IXH60_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1991560.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT         1186 a          506 c          694 g          990 t
ORIGIN      
        1 actagaatta ctagatggat ataactctga tttgatttca gagtataaaa atgagattga
       61 aacatgtgta aacgaaataa gaaaattaaa ttgggatatt gtttctgtga aaaaagaaat
      121 acaagataag gcgaaggtag agcttgaaat tgaagattta aaaaaacaag tagaaaagct
      181 atctcataag agttataaag atgtttatga tgagtattca aaagagacag atatttatag
      241 ggaactgaaa cgtgatgtgg aggcacttaa ggaaattccc aaaaatctaa ctgatagtgt
      301 tgacaaaatt gactttggca atggtataaa agttacagac gaaattgatg aacaccgcgg
      361 agaattgatt aagaatttag tgaccaatcg ggctaagatt caaacaataa ttaatgaaat
      421 gtcagatgaa attgatagtt atagagcaga gctgaataaa tcagattgga aagttaacta
      481 taatgaggtt tcaaagaggt atcaaagagg tatcaaaaac ttagtgaaga gttatctcaa
      541 gatgaattaa aaatgttaca aaaccttgat tctttatatc aatctttatc aaaaactgaa
      601 gagcgattgg aagaaattat tcaacaagaa ataaagctta aagattacca agttgaggtt
      661 gactcagcaa tgtcaagaat gaataaatca tatcaaaatt tctattctga gagaagtagt
      721 tcctcgcgcg aaattttttc tgatattaca ggaatagagg ctactattca accaaatagg
      781 gatttcaata attatgttaa agagctcaga gcttgtatta ataaggagga cacttttgac
      841 agtgagtttg gatctcttat tgataagctg acaaaaaatt ccattactaa tcaagatata
      901 tataatgaca tacttgatat aagagttagt ggggaaagta gtattttttc agataaaaaa
      961 ttaatcaatt ctataagaaa attgaatgat gcacaagttc ttgaattaca aacccttaag
     1021 ccaaatgatt tagtagttat ccgacttgaa ctaaatggaa aaaaagttag tttatcgaat
     1081 gctagtgctg gtcaaaagac atctgcaatt ctttctatga ttttggcgta tggaaattca
     1141 ccactcatat tggatcagcc tgaagatgat ttggatagtc aattaattaa taacttaatc
     1201 gtaagaagta tcattacaaa aaaggaaaac agacaaattt taattgtaac tcataatgca
     1261 aatatcccag ttaatggtga ttcagaatgg ttgattagca tgggagattc aaaagaaata
     1321 tcggctgatc agtgcgggtc ggtagatgaa cccaatatca agccaagaat atgtactgtg
     1381 atggaaggtg gcgaggatgc ttttacaaat agagcgaaac gctatggttt taaacaaatt
     1441 gtagaataac actagagggt gagacatatt attatgtccg ccctttttag gaggaaaaat
     1501 gaaaaaaata aaaagctata ccagtatctg ggcagttgaa aaagtcattt acgcaatcaa
     1561 tgattttcag ttgccttttc ctgtgacttt taaccaaatg gcttggttcg ttttatcttt
     1621 actgtttgtc atcgtatttg cccatgtgcc tccactatct atgatagagg aggtcttttt
     1681 aaaatactta ggcataccgg tggctgtcac ttggtttatg tcgcaaaaga cgtttgatgg
     1741 caagaaacca ctgggatttt tacgatcctt tatcagctat cacttgcgat ttaaggtgac
     1801 ttttgcagga aagaaagtca aagaacagaa gaaacgatgg gatgaaccca gtactcttgt
     1861 aaggagtgtg aactatgtat ccgattaagt atattgaaaa taatctcgtc tttaaccaag
     1921 aaggcgagtg ttttgcctac tatgaattag tgccttacaa ttactccttt ttgtcacccg
     1981 aacagaaatt tcaagtgcat gataactttc gccaactaat tgcgcaaaat cgtgaaggca
     2041 agatccatgc gttacaaatt gccacagaaa gtagtattcg cgccacacaa gaacgatcaa
     2101 aaaaagaaat taccggtaga ctcaaagaag tagcgaaaca acgaattgat atacagacag
     2161 atgcattagt atccatgatt ggcgatagtc agattgatta tcgctttttt attggcttta
     2221 aactaattgc gacagatgaa gaagtcaatc taaagagtct caagaaatct ttcttttctg
     2281 ggcttcaaga atttgtctat ggagtgaatc atcatttgat gggcgatttt gtttccttat
     2341 ccaatgaaga aattcgctgc tattctaagc ttgaaaaatt aatggaaagc aaattggctc
     2401 gaagatttaa agtcaggcga gtcacaccga gtgacctgac gtatttgatt gaacatattt
     2461 atggcgaaaa agggataccg tttgaagaat acgaatttca gctaccaaag aaaaagctaa
     2521 aatcagaaac actggtgaaa cgttatgact tattacgtcc aaatcgttgt ttaattgaag
     2581 aaaaaccaag gtgcttacgg atggaacatg agaatcatga gtcgtacgta gcgtatttaa
     2641 caattaatac aattgtgggg gagatggaat ttccttcctc agaacttttc tattaccagc
     2701 aacaacaatt tacattccca atcgacacaa gtatgaatgt agagattgtg acgaataaaa
     2761 aggctttagc taccgttcgt aataagaaaa aagaactgaa agatttagac aatcatgctt
     2821 accaatctga caatgaaacg aattccaatg tcttagatgc tttggattcc gtggatgaat
     2881 tggaaaccac cttggatcag tcaaaagaat ctatgtacaa actgagttat gtggttcgag
     2941 tgagtgccga atcagtggat gaactaaagc gacgctgtga tgaagtgctc gatttttatg
     3001 atgataccaa tgtaaaacta gtccggccct ttggcgatat gatggggcta catgaagaat
     3061 tcttaccctt aagtaaacga tatatgaacg actatattca gtatgttacg tctgattttt
     3121 tggcggggct tggttttggg gctactcaga tgctaggaga attagaaggg atttactttg
     3181 gctacaatgt ggatacaggt cgtaatgttt acctgaaacc ggcattggct tcacaaggag
     3241 tcaagggttc agtaaccaat gcgctggccg ccgctttcct gggttctctt ggtggaggta
     3301 aatcctttag taacaatctc ttagtctact atgctgtttt gttcggtggt caagctgtga
     3361 ttgtcgatcc aaaagg
//
LOCUS       DACEZN010000215         3368 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEZN010000215 DACEZN010000000
VERSION     DACEZN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.215"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXH60_002680"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXH60_002680"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1991561.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IXH60_002681"
     CDS             complement(2260..2511)
                     /locus_tag="IXH60_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1991562.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IXH60_002682"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IXH60_002682"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1991563.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          919 a          739 c          626 g         1084 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaa
//
LOCUS       DACEZN010000216         3362 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEZN010000216 DACEZN010000000
VERSION     DACEZN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.216"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(297..659)
                     /locus_tag="IXH60_002683"
     CDS             complement(297..659)
                     /locus_tag="IXH60_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991564.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(663..980)
                     /locus_tag="IXH60_002684"
     CDS             complement(663..980)
                     /locus_tag="IXH60_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991565.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQIGVIYRNKSKG
                     KEL"
     gene            complement(1081..1314)
                     /locus_tag="IXH60_002685"
     CDS             complement(1081..1314)
                     /locus_tag="IXH60_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991566.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(1311..2285)
                     /locus_tag="IXH60_002686"
     CDS             complement(1311..2285)
                     /locus_tag="IXH60_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1991567.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(2734..>3362)
                     /locus_tag="IXH60_002687"
     CDS             complement(2734..>3362)
                     /locus_tag="IXH60_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1991568.1"
                     /translation="SAEMRYISKQREKNEREKALVASEKAKIEEEKKSIIDLEKELAV
                     VFQKDPVPLNSFEKLSDGSYRLSSADFVDLYQKASNATNSKKYADMANESLGNTLDKN
                     IALFDKNMALEQENSFLKNQQKVDVSAVVAENKKLKEENRSLRRRLTQLKNAFQKSIT
                     RLSIRFGLVEKDMGISEELKILDQEKTLASQQNAPERNIGRSENEMEW"
BASE COUNT          971 a          739 c          472 g         1180 t
ORIGIN      
        1 atcagttaac tcacgattat tatgagaaat acttgtattc ttcgttcctt ttttaaatga
       61 aatactaaac cccaagttaa aaccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      121 ctttctgtta ctctttgtca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      181 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      241 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      301 cgtaaactca cgtcattaaa cattgacgta aatttactct cactcatctg acttttttta
      361 ctcggtatca ctccgagtaa gccgtcagat tcattgggat aatctcgttc gcttggcgct
      421 gatttttcat cagcaagcca ctcattatcc actgattttt cgtgttgctg atcgtcagaa
      481 attgacgata accaccgctc aaaatcatca aaagattcaa atccaaaatg cttccgaagt
      541 gccttaccca tttcagcatt ttccttctca atcaatcgtt ttttctgttg acgaagtttt
      601 gcctgttctt ttttcaattt ttcttcttgt gctttaatat ctgctaaagt cattcccaag
      661 atttacagct ccttgccttt tgatttattt cgataaataa caccaatttg atttaattgc
      721 ataacataag ttaaaatatt cagccaaaat tccttttcag attcatcttc tgtttcggct
      781 attttaccgt aaacaaattt atcagtaatc aaagaagaat ttgctaggcc tacattgatt
      841 gtagaattga gaacgtccat cattctctca agcatttcac tataattatc ttcatccggc
      901 aaattcaaat aatcagctaa tcgttcatac atcaattcaa ttctatcaac tgtctcttct
      961 cttaaatcaa ttttatccat gttacacctc atttccaaaa ctgccaccat ttccgattct
     1021 ttaattttcg atacacctaa ttcatcagca agatttttta tggtcatctt ttctgacaaa
     1081 tcagtcatta gcttctccac tactttcagc taccgcataa atatatttaa cggcgtgctt
     1141 agggataata atcggagagt caattttatt atttttcaaa atcaattcat attgcgttac
     1201 atcaagtaac ttaccggaca tttttttgct atccaaaaaa acaaccacaa cgtaatcacc
     1261 tgttaaattt accaaccaat ttttcttacc aaaatacttt tgtttttctg tcatttttct
     1321 tcctcctctg ttacgtctag ccctagaagc catgtgtcta attctttttt taaatcgtct
     1381 gccgattctg caaaggtttc tcgtaaatga ttcgtcaatt catcattttg actttcttgc
     1441 ttcagcaaat ttcgatattt atattttgtc tttcgattaa tttgccccca ataatcatgt
     1501 tctgttgtta aagcagtaat catcattttg tcagctacac ttgtatcagc aggcaagtaa
     1561 tgaggactag caaaactatc taagctttca taaaccatag catgccaatc agtcgctttc
     1621 cctcgtctta attgtaattc taaccgccac caactcacga tttcttttgg cacaattttt
     1681 cttttcttct cttgttccag ttttttatta tacatccgaa tttgacgttc agaagagcga
     1741 gatccccaat atgccgtctc taattttcca ttccgtccat aaataggctt aaaagaaact
     1801 ggatcaacta cccgatattg tgtaataaac tcatcaggta catcgataat gtcgcaagca
     1861 atatctgcac gtgaaaagtg aggttcgata aacaaatcat gaataaaatt tttcatggaa
     1921 ctagctaaga attgactaat cttattcgga ttgaagtcta tacgcccttt cccctcttgc
     1981 cattttaaaa tttcaatata agcaatattc tcttcatgtt ggtctaacaa ctgccaacca
     2041 ttattactaa cagatcttaa ataaccgtta ccttcattta ggcgcataag ctgttcaaaa
     2101 tctaaaatca acacatcatg tgtagtgtga taatagatat tctctttaag atttccaaca
     2161 atggtaattc ggtcgattga ccactgaatt tttagggggg tgtccataaa agggacactt
     2221 aaccgcctgt tagagggggc tgttaatttt tcccactcat ttatgatcgt gcctttttta
     2281 ctcaaattta gttcctcctc aaagaaaaaa gagattggtg gaaagaattt tccagagttc
     2341 acgtaactct aaaaaattca taaccagcaa tctctagtct tgaagaaacc tgaaattatt
     2401 cgtataatga atttgagtta cggagctagt gtgattagtt tccgtaaagg tcgagaacgt
     2461 gtccagcgtt gctcgacttt atttttttgc tcttttttca gtttatttat ttgataactc
     2521 aataatagca cttctcttta gctatgcaaa tatccaaaaa cataaaaccg caccctttga
     2581 aaagggcgcc cccaaacttc gctaacgcta agttggccaa aaaatggact tgccattttt
     2641 tagcaaatgt cagcttcagg gattagtggt ttagccgtca actccttatc ctcggcacag
     2701 accgcctccg gtaccgttga ctgcaccgta taactaccac tccatctcat tctcagaacg
     2761 ccctatatta cgttctgggg cattctgctg actagccaac gttttctctt gatctaatat
     2821 ttttagctct tcagatattc ccatatcttt ttctacaagc ccaaaacgaa ttgataaacg
     2881 agtgattgat ttttgaaatg cgtttttcag ttgagttaac cgccgacgca aagaacgatt
     2941 ttcttccttc agctttttgt tttcagcaac aaccgctgac acatcaacct tttgttgatt
     3001 tttcaaaaaa gaattttctt gttctaacgc catgttttta tcaaataaag cgatattttt
     3061 atctaatgta tttccaagcg attcattcgc catatcagca tattttttgc tgttcgtagc
     3121 attagacgct ttttgataca aatctacaaa atcagcacta gataagcgat aagaaccatc
     3181 tgataatttt tcaaaactat tgagtggcac aggatctttt tgaaatacaa cagccagctc
     3241 tttttccaaa tcgataatcg attttttctc ttcttcaatt ttcgccttct cagaagccac
     3301 cagagccttc tcacgctcgt ttttttctct ttgcttagaa atatacctca tttccgcaga
     3361 aa
//
LOCUS       DACEZN010000217         3325 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEZN010000217 DACEZN010000000
VERSION     DACEZN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.217"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..560
                     /locus_tag="IXH60_002688"
     CDS             <1..560
                     /locus_tag="IXH60_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1991569.1"
                     /translation="LGNLVGLLLTVLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLY
                     FGLPYQLSALTASVICFTITSSAFIGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYI
                     VLPQAFRISVPALSNVAMDLVKGTSLAATITVPEIFQKAKIIGGRTFDYFSLYILVAL
                     IYWVICIGIEKVQQYLEKRSMHQSI"
     gene            686..1375
                     /locus_tag="IXH60_002689"
     CDS             686..1375
                     /locus_tag="IXH60_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991570.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            1381..2337
                     /locus_tag="IXH60_002690"
     CDS             1381..2337
                     /locus_tag="IXH60_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1991571.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            2381..2893
                     /locus_tag="IXH60_002691"
     CDS             2381..2893
                     /locus_tag="IXH60_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991572.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(3149..>3325)
                     /locus_tag="IXH60_002692"
     CDS             complement(3149..>3325)
                     /locus_tag="IXH60_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1991573.1"
                     /translation="SRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSLLVVSNDGA
                     FQEAADKIKKGLLI"
BASE COUNT         1070 a          607 c          677 g          971 t
ORIGIN      
        1 ttttaggaaa tctggtaggg ttgcttttga cagttttagg tcttttggat tggctgcctc
       61 tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca ctagtcctgc
      121 tgtttctgtt atattttggg ttaccttatc aattaagtgc cttaacggct tcagtcatct
      181 gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca ctagcaggcg
      241 tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt ggggtattga
      301 aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt aacgtagcta
      361 tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa atatttcaaa
      421 aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt ttagttgcac
      481 tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta gaaaaacgtt
      541 ctatgcatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca ccatttctat
      601 tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc
      661 gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa tagaatcctc
      721 gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca cgatgcctga
      781 tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa gccgagaatg
      841 gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca aactgtcatt
      901 tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag taaaaactga
      961 aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag aggagatgcc
     1021 tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga
     1081 agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa aagcaaagca
     1141 aatggagcga ggcgtaaaat attttccaac agatcaattg tacaaagaat tgaaaagacg
     1201 ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt tagaggaaat
     1261 agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc atgattatga
     1321 atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg agtgaaaaat
     1381 atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa agaagcactg
     1441 tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg tgattccgct
     1501 acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atcgaatcgc tgtgatccaa
     1561 aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg gacagtcaaa
     1621 gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag tgaagtactg
     1681 gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga tacagaggtg
     1741 tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag tcgctttcgc
     1801 atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt
     1861 gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt tttccacgca
     1921 gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt cgataaaggg
     1981 ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg caatatacgc
     2041 gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt gaagtttctt
     2101 gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact cttgattact
     2161 tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg actgggagtg
     2221 ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct agatcatcca
     2281 aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat ccaatgatta
     2341 gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc ggcaaacaag
     2401 gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt tcattgcttc
     2461 gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga ttttgaacga
     2521 tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg gaacagctgc
     2581 attagtctca agcgtggatc ccgtttacac aaaaatagat ggtagttgga aagaatcaac
     2641 tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact
     2701 agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata tccaagatat
     2761 ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt ctgctggatt
     2821 cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag catatcaaat
     2881 aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca ctactgaaaa
     2941 aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa aaaacaagaa
     3001 agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt acgaaatagc
     3061 tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac gacctctcgg
     3121 cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt attttatctg
     3181 cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata gctttcaaac
     3241 gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc tgttcagatg
     3301 aatctccacg ttttttcagg cgcga
//
LOCUS       DACEZN010000218         3298 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEZN010000218 DACEZN010000000
VERSION     DACEZN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.218"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            234..2000
                     /locus_tag="IXH60_002693"
     CDS             234..2000
                     /locus_tag="IXH60_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1991574.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2102..>3298
                     /locus_tag="IXH60_002694"
     CDS             2102..>3298
                     /locus_tag="IXH60_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1991575.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKT"
BASE COUNT         1166 a          535 c          701 g          896 t
ORIGIN      
        1 cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag
       61 tacaggaagt agactaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa
      121 aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg
      181 cttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata
      241 cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat
      301 ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag
      361 atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc
      421 taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac
      481 agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa
      541 tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt
      601 ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa
      661 tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt
      721 ggggaagtaa agatccagac cgagaagact ttttcggggg agatttgcaa ggtgtgatgg
      781 atcatttgga ttacttgacg gaccttggca tcaatggcat ctatttttgt cccatattta
      841 aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg
      901 gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca
      961 tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca
     1021 aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt
     1081 cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt
     1141 ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg
     1201 atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta
     1261 atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag
     1321 acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat
     1381 tccatgcagt gatgaattat gcatttacag attcgattaa agattatttt gcaaaaaaga
     1441 aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc
     1501 aggtgaacga aggaacattc aacttattag attctcatga tactgccagg atcttgaccc
     1561 tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag
     1621 ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt
     1681 gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa
     1741 aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt
     1801 ggctaatagt aaatgatcgt gaagataagc tctatttcac acggaaacta gagggacaga
     1861 tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg
     1921 tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg
     1981 gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga
     2041 agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa
     2101 gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg
     2161 cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa
     2221 aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac
     2281 tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt
     2341 ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata
     2401 tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga
     2461 agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga
     2521 acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc
     2581 atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga
     2641 agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt
     2701 aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg
     2761 tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt
     2821 ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat
     2881 gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa
     2941 caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc
     3001 gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca
     3061 aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa
     3121 tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac
     3181 aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg
     3241 cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaaca
//
LOCUS       DACEZN010000219         3292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEZN010000219 DACEZN010000000
VERSION     DACEZN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.219"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            37..588
                     /locus_tag="IXH60_002695"
     CDS             37..588
                     /locus_tag="IXH60_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1991576.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1530
                     /locus_tag="IXH60_002696"
     CDS             1264..1530
                     /locus_tag="IXH60_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991577.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1588..2100
                     /locus_tag="IXH60_002697"
     CDS             1588..2100
                     /locus_tag="IXH60_002697"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1991578.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="IXH60_002698"
     CDS             2097..3134
                     /locus_tag="IXH60_002698"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1991579.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          653 g          871 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac ac
//
LOCUS       DACEZN010000220         3262 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEZN010000220 DACEZN010000000
VERSION     DACEZN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.223"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(259..1344)
                     /locus_tag="IXH60_002699"
     CDS             complement(259..1344)
                     /locus_tag="IXH60_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1991580.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIVKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(1664..2071)
                     /locus_tag="IXH60_002700"
     CDS             complement(1664..2071)
                     /locus_tag="IXH60_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991581.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(2090..2908)
                     /locus_tag="IXH60_002701"
     CDS             complement(2090..2908)
                     /locus_tag="IXH60_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1991582.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          986 a          506 c          436 g         1334 t
ORIGIN      
        1 attacctctc ttcatgaatt attataactc aaaaaaatac atatgcaaac actagttccc
       61 tgtttttttg aatgaaaaat aactaaaata tctaacgatt atgacattaa aacctacgtt
      121 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataataacta tttatttgta
      181 acatttgtaa taaaattaaa attgtaaaaa agttctaatt taaaattgat catttttata
      241 gtgttcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      301 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcaa taaaccagtt
      361 ttttacatac acaaaaatca aattctgcaa attatttact ttctcagtct tttgtttttg
      421 ataagctttc agcaacgtca tatataatcc tttatccgca tcaattccgt aagattcaag
      481 ttcttcatac acaactaccg tcttctcaag ttcctcggct ttatgttttg ctttgtgtat
      541 tgtggtgacg gtttgttggg cttcttcgta agtgttagaa aatgttttta tcaagcttaa
      601 taccttttct ggtacaaatg tgtttatgct gtcttctttc atgatttcaa cgaagttatc
      661 tagcaaaact tgattttgta attggtctgt ttcagtgtct tttaagtgtc ttttagtgtc
      721 taaagattta ttaatataat aatcttgtgc gatttccgca tttccagtgt tatcaagggt
      781 ttcagctgtt ttttctcgac gtgcgatttc cgcatttcca gtgttatcaa gggttttagc
      841 agtttcctct cgacgtgcga tttccgcatt ccgagaagag gttgatttaa caatagtttc
      901 gcttaaatcg tcatcgaaag aattttgaat aaaaagatca tcggcactta tttcaggttg
      961 aagtaaatag tatttatttt tttcattttt tttcgttttt gggttaaatt ctccttttac
     1021 gactgaaatc aaattatatg tttctaactc gtttaaaatg gtttttactt tgccttggta
     1081 gcaatttaat ttgtccatta ggtctgcttg tgtaaattcc aaatataata gaccatcatc
     1141 gtcgatccat ttttcatgta aggctctacg aaattcattt ttaaaaagca tataggctat
     1201 ttttgagctg tctttcattt tagagtattt agaattctga attaaaaaac taggaaattt
     1261 ataatatttt tcaatataaa cattcgttgc tttatatccc caatttttaa ttatcatttt
     1321 ttgagattga ttttctgtag acattaaatt tctccttctt cttagaaact tgtatatatt
     1381 caggattttc aacaagtgaa ctataccatg tattaagggt gaataaaaca ctttcagtat
     1441 ttaaggtata agttatatat taaaaaaata agtataagtt atactttaaa tacaaataat
     1501 gtataactta tactttaaat aataatttta taatgttgat ttaatcgcaa atttacacgt
     1561 tgtttattaa gttatacctt aaatatttaa tgtataactt atactttaat attcatttat
     1621 ctagtataac ttatacttta attgaatacc gcatgaaaaa aaattatttt ggtaattttt
     1681 caaactcttc ttgtatttct tctcttatgt gttcttcttt tgctactcct agtctatgct
     1741 ggatatatga gtctgctaat gtattaacaa ttttttcgaa agaaattctc cctttttcac
     1801 tttgtaactc cgtcatatag tcttgtaaaa ttgagagttt gctatttaat gccggagaga
     1861 tctttttagg aactgatacc ttgtttcttt tatcaggaat tgaaactggt cggcctcttt
     1921 ttttcaatgt aatatttctt gtgttactca atgcactatt gctaaagtta tattcaccgc
     1981 ttattttcac atcttggcgt ttgttattgg gtaataatgg ctcaaattca ccattctcca
     2041 tcattttagg aatatcaaaa tctttaggca ttacttatgc aactccttat tatttattta
     2101 aaaactcttg tctagcatct agttctgcca aaatatttat aaatacttgg tgtgctttat
     2161 aatcccattg atcaaaattt ccatttttgt attttcttaa atgtatacca attttagaat
     2221 accttctcaa tcttttttga taatgaatga gatttttgga taccacggtt ccgtattttt
     2281 caattgcttc ttctaggatt tcttcatcaa aatcatcatt tggttctacc atacaaggta
     2341 caatatctat aatttgcaaa tcaagatcgt atctatcgac catgaatttt tgataattta
     2401 catatttgtt gactccctct aaggattctt cagctgtttg aaatgaaatt atcgaatagt
     2461 ctgaagctgc catagcatta tcggaaaatt cgctaatggt tggtggtaca tcaataaata
     2521 tttcttgata attgttcttt attggaagaa gtaatttttt caagacagta acttggtcta
     2581 attcattttc atgtttatta gcaaaagcgg agaatgatcg aaaactagtg tcacatgcca
     2641 gaaggtcaag attttcattt accttaataa tactacttgc taattctcca tttgcgacac
     2701 catttgttat agtggttttg atatcggtta taccgaaagt tcttgctatg acttgtgtta
     2761 agttcgcttg agggtctaaa tcaataataa gcgtttttct atttcttatc attgcattgt
     2821 cataagcaag catgctgact atcttagttt tacctactcc tcctttaaaa ttgccaactg
     2881 taaatgtagt cccttgaaaa gtatccatgt aaatcctccc aatctttaat tgataattaa
     2941 agtataactt atatatcgga aatggtcaac attaaaagta taacttaaat aataatatct
     3001 aaaaacgttt ggatatagcg gtttttaata ataaaagtat aagttatacc atatatattt
     3061 atggtataac ttatactttt attattaaag gtataactta taccttaaat attgcttttc
     3121 gaaaatagct attctagtct atctgtttct ttacttttcg ctgctgattt gaattgaatt
     3181 gatcatattc attttctagt attaataact tttcgtatac atcggtcata ttcatactag
     3241 aagcaagact tagatcatat ac
//
LOCUS       DACEZN010000221         3199 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEZN010000221 DACEZN010000000
VERSION     DACEZN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.224"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            301..585
                     /locus_tag="IXH60_002702"
     CDS             301..585
                     /locus_tag="IXH60_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991583.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="IXH60_002703"
     CDS             588..1004
                     /locus_tag="IXH60_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991584.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="IXH60_002704"
     CDS             complement(1141..1860)
                     /locus_tag="IXH60_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1991585.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="IXH60_002705"
     CDS             2513..2608
                     /locus_tag="IXH60_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1991586.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="IXH60_002706"
     CDS             complement(2910..3155)
                     /locus_tag="IXH60_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991587.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DACEZN010000222         3131 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEZN010000222 DACEZN010000000
VERSION     DACEZN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.225"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            75..503
                     /locus_tag="IXH60_002707"
     CDS             75..503
                     /locus_tag="IXH60_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1991588.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="IXH60_002708"
     CDS             490..732
                     /locus_tag="IXH60_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1991589.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="IXH60_002709"
     CDS             complement(1032..1247)
                     /locus_tag="IXH60_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991590.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="IXH60_002710"
     CDS             1354..1668
                     /locus_tag="IXH60_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1991591.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2949
                     /locus_tag="IXH60_002711"
                     /pseudo
     CDS             1772..2949
                     /locus_tag="IXH60_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         1034 a          474 c          630 g          993 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa ttgttgcaag caatgacaag tttagtgttg taggtgagca atggttccaa
     1981 tattaccgtt cgttaaatga acaaaaggaa ataacctatg ataagcgaga ggaacaatta
     2041 aaaattattg ttcgatggat tggtgataaa aagttgtctg atttgacacc agagtgcctt
     2101 caagaattgc tttttacctt aaaggaatgt ggcgttaatg gaacaaatgt tggctatgcc
     2161 aaaaatagtt tacaatcact tattcaagtt ctaaatatga tttttaaata ttgtcaaaga
     2221 aagaatttga taagtgaaaa tccaatgaag tctgtgagaa tgccaaaata tcagttaacg
     2281 gtaactgatt taaaagaagc cataaataat attgaggaca agtttcttac tgtcgatgaa
     2341 ttgagaactt ttttgaatta tggcattgtt catgaggaat taccgatgtc tgtacttttc
     2401 catgttctat tttacactgg ctgccgagtc agcgaggcgc ttgctctaca gcctggagat
     2461 atagatttca aaagaaatga aattctgttc tataagcaaa cggcagttaa aggtaagagt
     2521 aaggattttc gaattgaaac tactaagaca gttagttctg ctagacgagt tctagtaact
     2581 cctctagtta tggaaaagct acaacagttg attaaggctt tgggtgagat gaggcaacat
     2641 tacaggttta ttatagaaga aacatatttg tttgtgtatc ttgattctag taaacgtggt
     2701 gttccttatc gtcgagagta tgtaaatgat cacgtcaaac ggtgcgtgaa tcgctcagga
     2761 attaataagc catttcatac tcatttagct cgtcatacaa tggcaagctt agtagcggaa
     2821 cactgtagct gggaagtctt gaaagatcgc ttaggccata ctgaccaaac aacttcgaaa
     2881 atttatcgcc acttaacgtc aaatgaaaag gtaaaacctc tgattgcttt tagttcttta
     2941 gagagttaaa aaaacggtga aagcactggt attacaggat tcttgaaaaa cgatttttta
     3001 acaaattagt caatatgtag tcagaggtaa taaaaatcct tttatatcaa tactttaacg
     3061 acttttcaca agtatcaaag aaatccgtcg ttaatcattg ttatcagact gagaaagaac
     3121 ccttggtatt c
//
LOCUS       DACEZN010000223         3101 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEZN010000223 DACEZN010000000
VERSION     DACEZN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.226"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(559..1599)
                     /locus_tag="IXH60_002712"
     CDS             complement(559..1599)
                     /locus_tag="IXH60_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1991592.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="IXH60_002713"
     CDS             complement(1948..2274)
                     /locus_tag="IXH60_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991593.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3049)
                     /locus_tag="IXH60_002714"
     CDS             complement(2261..3049)
                     /locus_tag="IXH60_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1991594.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          927 a          536 c          434 g         1204 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt g
//
LOCUS       DACEZN010000224         3055 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEZN010000224 DACEZN010000000
VERSION     DACEZN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.227"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(792..1133)
                     /locus_tag="IXH60_002715"
     CDS             complement(792..1133)
                     /locus_tag="IXH60_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1991595.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1133..1363)
                     /locus_tag="IXH60_002716"
     CDS             complement(1133..1363)
                     /locus_tag="IXH60_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1991596.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1650..1988
                     /locus_tag="IXH60_002717"
     CDS             1650..1988
                     /locus_tag="IXH60_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991597.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2193..2432)
                     /locus_tag="IXH60_002718"
     CDS             complement(2193..2432)
                     /locus_tag="IXH60_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1991598.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2408..2755)
                     /locus_tag="IXH60_002719"
     CDS             complement(2408..2755)
                     /locus_tag="IXH60_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1991599.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT          962 a          563 c          480 g         1050 t
ORIGIN      
        1 acaaaaagtg actcaaaata aaaaaaacct tacattatac tggaatgaac tgtattgata
       61 gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat tttattaatt
      121 atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt acattcataa
      181 ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat tttcgatcat
      241 tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt tttcagaatc
      301 tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca ttgtttttct
      361 tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct ttcctagaca
      421 gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg acattactca
      481 tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt tcttctttct
      541 gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt
      601 gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt aaaatgctat
      661 atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta gaatgaacga
      721 catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat tatagagttt
      781 acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat gtcttgcaat
      841 ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt taattgtgta
      901 ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt cggtaaattc
      961 tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt aaattcataa
     1021 cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga gggatcaaaa
     1081 tcaatccata caatatcacc ttttttagga atataaattc tttcttctga cactataaaa
     1141 tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac tcggcctctt
     1201 ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca tcatcagaat
     1261 acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa ggtaaagtta
     1321 ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc actcccttaa
     1381 taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa
     1441 attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt agccagttca
     1501 ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac
     1561 ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga
     1621 aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt
     1681 ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt
     1741 caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa
     1801 ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga
     1861 ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca
     1921 gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag
     1981 agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa
     2041 caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc
     2101 ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt
     2161 tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc
     2221 atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt
     2281 tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca
     2341 gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca
     2401 atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca
     2461 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     2521 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     2581 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     2641 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     2701 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     2761 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     2821 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     2881 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     2941 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     3001 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaag
//
LOCUS       DACEZN010000225         3025 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEZN010000225 DACEZN010000000
VERSION     DACEZN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.228"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            164..943
                     /locus_tag="IXH60_002720"
     CDS             164..943
                     /locus_tag="IXH60_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1991600.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            936..1253
                     /locus_tag="IXH60_002721"
     CDS             936..1253
                     /locus_tag="IXH60_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1991601.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1497..>3025
                     /locus_tag="IXH60_002722"
     CDS             1497..>3025
                     /locus_tag="IXH60_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1991602.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     G"
BASE COUNT          837 a          537 c          621 g         1030 t
ORIGIN      
        1 taaatatgac ccaaattatt ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat
       61 gagaaaaata taatcttgtt tttaaataaa acggctacaa tttagcgaat ttatgttaca
      121 atgagtccgc tgtttatttg ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag
      181 tgcgccatgg cttcaagctt tgcatgcagt gatgccactt tgtataagct atataccagt
      241 aggattagct tgcggagtat tgttgcaaaa agttggattt gatcctctct tatcaggact
      301 gttatcgatt ctggtctttt ccggtggcgc gcagttttta gttgcatcta tgcttacgac
      361 gcaagcatca tttgcaacga cattattgat ggttttcttt ttagaattac gctatacatt
      421 attaggagca agtcttgccg gttttatgaa aaaagaaaag cggacgtttt tggctgtctt
      481 ttctcaatca ctgaatgatg aaaactatgc ggtgaactac ttaaagtttt caactgaccc
      541 ttcctggaat aaaagaaagg ctttatatgt caattggttc tcaatgactg catgggccgg
      601 tagcaatatg atcggtaatt ttttcggctc ggtgattcga gtagatgctg accttgtcca
      661 ttttgctttg acggccatgt tcattttcat gtttatcatg cagatgaaaa gcgcactact
      721 gatctttaca ggtttattct caggcgtatt aggggtcgta ttcatggtat tattccaaaa
      781 tactttcgga ttgattgcag caacagtcat tgcttcattt gccggattta tgctggagaa
      841 ggcattcaaa aaagaaaaag ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc
      901 gctcttcaac ttcccggaac aggaggccaa tcaccatgac taaactatat ttgctacttt
      961 tagttgcttg tctgttcatc gtggcatata tcccgcgctt gttcccgatg ctttatttta
     1021 ctcatcgaaa agtgccatcc tggttcagtg actggatgaa atatgtaccg gtggcattat
     1081 ttgcggcgtt agcttttaaa gatgttttca ttactcatga acatctggat attgcttgga
     1141 atatcaaaat agctgcaatg attctagtag caggcgtggc ttacaaaaca cgttcgatgg
     1201 cgttgtcagt tttgacagga cttgcgtctg tctttttgct gtctatgctt taaactgttt
     1261 tattttatta aaaacagaga ttgcgacata agttgttcaa actgatctat ccgaactgca
     1321 tttgcaacag tcaattctgg atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg
     1381 cttatgatgc aagctctttt ttattttagg aattagagag ggatttgtcc ttctatgatt
     1441 ttttgctata atagggaaga tgtttatttt atgaaagaat agttgaggga atttaaatga
     1501 ataaaaaatt gatgcaagga acattttggc taacctttgc taatcttcta tgtaaagttt
     1561 taggggtagt ctatttgatt ccctggctaa gtatgatggg gaacaatcaa gatggtatgc
     1621 ttgctacgac attgtataat gttggctatc ttccttatgg attgttcctg atgcttggta
     1681 cagtcggatt tccaaatgcg attgcaaaaa aagtagctgt agccacaaaa gaaggagata
     1741 acgctgcttg tcggctgatt tttagaagta cgatcaatat catgtttgtc atcggtatcg
     1801 cctctgctgc actgatgtat ttatttgctc ctttactagc cggaatcagt ccgattgcta
     1861 acgttaataa tgggatacta gctatcagaa gtttatgccc atctttgatt gctatcccaa
     1921 ttcttagtgc aatgagagga tatttccaag gaaaaaatga tctgcggcct tatggaactt
     1981 ctttgattat cgaacaggct gtgcgagtga tcgttatctt ggctggtact tattacttgc
     2041 gcgtattgac tgacggaacc atattagaag ctgtattgat cagtacagtc gcttctttct
     2101 tcggtgggtt agctgcgatt atccatatgt atatcgtcgg acgcagaaaa gacttcttcg
     2161 aacttaaaga ttttttgatt tccagccgtt atttcgaaag agaaaaccga tctgcctctg
     2221 tctctatcat tcgggaaaca ctaccgttta tttttgtcgg ctcagtcatt actatcgttc
     2281 aaatgatcga ccaagtaacg atgaaaccac tgcttcactt tttccgtcca gagatcgcag
     2341 atcagcaact ggaattctta ttcagccgtg cttctgtcaa tccaaataaa ttgacattga
     2401 tcctgatctc catggtagga acagtagcta taagcagctt gccaatatta agcacgatga
     2461 agaaaaaaga tcggatacaa atcgaaaaaa cagtaggaga tagtttttcg attgcgttat
     2521 tgattcttct tccttcattg actggaatgt ctttattagc tggaccacta tacacattgt
     2581 tttttggcta tgatcctgaa agtgtcggat atttccaaat ggctttgcta gcttctttgt
     2641 ttttctcttt attcacgatc ctatctacga tgcttcaatc tttgaatcac catcgttttg
     2701 cgatccgatt aactgtagaa gcaattatcc tgaaagtcat cttccaagta atcggattag
     2761 gattagttgg cggatacggg atgagcttat caaatggctt agcattcggt attgtctttg
     2821 ttcgcggcta tcactttatg tgcaaagaat accgaatagc acctttagca aagatcagca
     2881 attttttctt aaaaactttc cgctccaccc tgattatgct tgcagtatgt tttgccgttt
     2941 tctttttgct gaatcagcga ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg
     3001 tggctgtagg aagtattggc ggctt
//
LOCUS       DACEZN010000226         2988 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEZN010000226 DACEZN010000000
VERSION     DACEZN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.229"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..696
                     /locus_tag="IXH60_002723"
     CDS             <1..696
                     /locus_tag="IXH60_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1991603.1"
                     /translation="YQRLSEETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVM
                     TKEPISVKKTMSIASVSHQMIWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQ
                     IADTISDQISGNIHTIDTDREGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVA
                     QKTMVMNQKRNILIEQVNLHYLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIV
                     SKAIVVCQVMERS"
     gene            711..1673
                     /locus_tag="IXH60_002724"
     CDS             711..1673
                     /locus_tag="IXH60_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1991604.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            1784..2122
                     /locus_tag="IXH60_002725"
     CDS             1784..2122
                     /locus_tag="IXH60_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1991605.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            2201..2908
                     /locus_tag="IXH60_002726"
     CDS             2201..2908
                     /locus_tag="IXH60_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1991606.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT          965 a          531 c          679 g          813 t
ORIGIN      
        1 tatcagcgtt tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat
       61 cgtttgatgg ggattgttac ggcaaaagat gttttaggga aatcgccaaa ccagatcatc
      121 gatcgggtga tgacgaaaga accaatcagt gtaaagaaaa caatgagtat tgcctctgtt
      181 agccatcaaa tgatctggga tgggctagaa gtcatgccgg tcgtttccga tgacttgaca
      241 ttggaaggct tgattacccg gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa
      301 ccgcagattg cagatacgat ttcggatcag atatctggaa atatccatac gattgatact
      361 gaccgtgaag gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc accacaaatg
      421 gtcaatggcg ttggaaccat ttcttttggc gtacttagcg aaatcatttc tgatgtcgca
      481 caaaaaacaa tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt gaacctacat
      541 tatttgcggc tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt attggaaatc
      601 ggcagacgtt ccgctaaact ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa
      661 gcaatcgttg tttgtcaagt aatggaacgt tcttagaaag caggagtgaa atgacagtac
      721 agcaagaaat agtagataca ataaaaaaat acgataaaat cattatccac aggcatttgc
      781 gccctgatcc tgatgcatta ggatcacaag taggattagc cgaactatta cgggaaagtt
      841 tcccagaaaa agaaatacgt caagtaggtg aaacgattga agggcttcgc tttttagcag
      901 aaatgcaaga agttgaagat gtcttctacc aaggagcgct agtcatcgtg actgatacag
      961 ctaattcacc cagaatcagt gatcaaagat atcagttggc agaaaaactg atcaaaatcg
     1021 accatcatcc caatgatgac ccttatggag atcttgtgtg ggtaaataca aaagcaagta
     1081 gctgtagtga gatgattgca gaatttgctt tgatgtttcc tgaatcttta aagatgaacg
     1141 ttaaggctgc acggttactt tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc
     1201 cctccacgac gtcccgtaca ttagagattg cttcgatatt gagaaattat ccatttgatg
     1261 caagtgcttt gaacagagag attgaacaaa tgccgatgaa agtcgctaaa ctttccggtt
     1321 atctttatca aaatatccag gtggatgaaa atggtgcagg aagaataatc cttccgcaag
     1381 aaattcttga taaatatgga atcgatgatt cagagacagc ttcgatcgtt tcacttcctg
     1441 gagtgattga cgaagtgctg gcttggggga tttttgttca gcagccttca gggtattacc
     1501 gcgtacgttt acgttctaag gggccgatca tcaatgaatt ggctaaacgc catcatggag
     1561 gaggccatcc attagcaagt ggtgcgaatg caaaagattt agcagaagta gaggaaatct
     1621 ataaagagat ccaagatata tgccaatcgt ataagagcag aaaaacagaa tgatacttcg
     1681 ttcgaaaacg tgccttttgt agtaaaaatc acaaatgtat cttttcaaga tcttcttttt
     1741 ccgttacaat agtgctcact aaagatttta aaggagaaag aagatgacat taccaaattg
     1801 cccagaatgt ggctcagagt acacatatga agatcgaggc ttgttgattt gcccagaatg
     1861 cgggaacgaa tggaacaaag aagaacaaga aactgtcata gaagacggat tagtggtatt
     1921 agatgcaaat ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta aagacttgaa
     1981 agtaaaaggt gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt
     2041 agaaggcgat cataatatcg attgcaaggt agaaggattt ggaccgatga aactgaaatc
     2101 ggaatttgtg aaaaagaatt aaagaatatt caaggtcttt tcacattctt ttcctactac
     2161 taatgataaa ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta
     2221 tggtttttta gatccgacat ttcttttggt cattcttgga ttagttattt ccggaattgc
     2281 gtcagcgtac gtcaatagta ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt
     2341 gactggtaca caagcagcac aatatatatt acaaagtcaa cagataaata atgtcggtgt
     2401 tcaacaaatt gccggagatc tcacagataa ctacaattca ggtaataaga tgttgagttt
     2461 gtctcaagca actgcacagt ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg
     2521 cggacatgcg gtacaagatg cttcgggtta tgtcccattg aagattcgtg cagcacttgt
     2581 tccagcagct aactttggtt cgatgatttc gttccccttg attatggtag gtgtattgct
     2641 tagctggaac tctacgttaa tcaatatcgg gattttcgct ttttctttag cattactttt
     2701 ccaattggtg acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag
     2761 tgaaggcgga ctactgacgg aagaagaagt accgatggca agacacgtat tatttgcagc
     2821 agcactgact tatgttgcag cagcgctttc gacattcctg cagctgttgc gcttgatctt
     2881 attgttcggc ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag cgctttgatc
     2941 tgagctaata ccgagagtta gctgattgaa taccatttat ttggaata
//
LOCUS       DACEZN010000227         2918 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEZN010000227 DACEZN010000000
VERSION     DACEZN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.230"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            204..1172
                     /locus_tag="IXH60_002727"
     CDS             204..1172
                     /locus_tag="IXH60_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1991607.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="IXH60_002728"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="IXH60_002728"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1991608.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="IXH60_002729"
     CDS             2202..2810
                     /locus_tag="IXH60_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1991609.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          709 g          787 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaag
//
LOCUS       DACEZN010000228         2904 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEZN010000228 DACEZN010000000
VERSION     DACEZN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.231"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            46..1374
                     /locus_tag="IXH60_002730"
     CDS             46..1374
                     /locus_tag="IXH60_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1991610.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            1576..2409
                     /locus_tag="IXH60_002731"
     CDS             1576..2409
                     /locus_tag="IXH60_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1991611.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2425..>2904
                     /locus_tag="IXH60_002732"
     CDS             2425..>2904
                     /locus_tag="IXH60_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1991612.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     "
BASE COUNT          965 a          447 c          650 g          842 t
ORIGIN      
        1 aagagtaaaa tacgaagtat aacttggatt tggaaggatg attggatgaa agctattacg
       61 cctgatatga caacaaaatt taacaaacag tttcaagcaa atatcaaaca gcaggcattg
      121 cagcgggcag ttgtgaagaa cggaattact gcttctgctg aaaatgtttc agcacatgtg
      181 gagaatacac cggtattttc tatagatttg gctacgggga aagtggctaa tcaaatgcaa
      241 tcggggcgtt gctggatgtt tgctgcactg aacacattcc gtcataagat gctgaatcaa
      301 ttccatttaa aagagtttga attatcacaa aattacactt ttttctggga taaatatgaa
      361 aaggcaaatt atttctatga aaacatcatt gcaacaggaa aagaggcaat cgatagccgg
      421 aaagtagctt tcttactcgc tactcctcaa caagatggcg gccaatggga tatgatcgtt
      481 tcattattcc aaaaatatgg cgttgtgcct aaatcggtga tgccggaaag tgcgaatagt
      541 tcaaattctc gagatttgaa taactacttg aacaaattac tgcgtaaaga tgcagtagtg
      601 ttacggaaaa tggttgctca aggagaaaca ttagacgcta ttgaagaaaa gaaagaagaa
      661 atgctagaaa gcatttataa cttcttagct atctcattag gaacacctcc aaaagaattc
      721 gattttgagt accgcgatga agaaaagaac tatcatcttg accgaggact gacaccacaa
      781 attttttatg ataaatacat cggtgtaaaa ttagatgatt atgttagcgt gatcaatgca
      841 ccaacaaaag acaaaccatt caatcgttct tatacagtag agatgctagg aaatgtcgtt
      901 ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa ctttcaaaaa attagcgatt
      961 gctcagcttg aagaaggaga atctgtttgg tttggttgtg atgtaggtca atcttctact
     1021 agaactagtg gcatcatggc cttagatgct tattcaatgg atgatctatt tgatacggat
     1081 tttacaatga ctaaagctga acgtttagat tttggagaaa gtctgatgac ccatgcgatg
     1141 gtattgaccg gtgttgactt agtagacggg caatcaacaa aatggaaagt cgaaaatagc
     1201 tggggcgaaa aagttggaaa gaatggcttc tttgttatga gtgatgcctg gatggacgaa
     1261 tatacgtacc aaatcgttgt aagaaaagat ttattgacga aagagcaatt aaatgcgttc
     1321 aatgaagaac caattgttct atctccgtgg gatccaatgg gtgctttagc atagacgagt
     1381 atttcaatga agagtatgta gtaaagataa aaagagctgg cttaagccag ctctttttat
     1441 ctttaaagtc cattccctgt tttttatggt ccctgtgcta taattctaaa taaaggataa
     1501 ggattggaaa taatcctttt atcattaaaa atatgatgac gaagtagaat aaaattggga
     1561 gtagggggga aagtcatgga tatccaaact gttttatctg atctgaaatt aaatggtaaa
     1621 gtgattccag ttgttggtgg cgatgttaat cagacctatc gtataaaaac cgaacatcgt
     1681 gcttattttt taaaaatcca tccgaatgta aaaaaaggat tttttgaagc agaagtagac
     1741 ggattaaaag aactgtcagc attcgtacgt gtaccggata cgtatatgct aggtgaaaca
     1801 agtgaaggcg cttatttgtt aatggaatgg atcgagccgg gaaaagggga tcaacgagat
     1861 cttgctgcag ctttagctaa tctccatcag caaacagcgc cacaatttgg ttttcgtaaa
     1921 gataactatt tgggtacact tgttcagaaa aattcttttg aagaagattg gtggactttt
     1981 ttctttaaag atcggcttga atcccagatt tctttggcag aagaaacgaa tcgatggaat
     2041 gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg tattgaaatc ggttgaacca
     2101 aaaaagatca ctccacgcct cttgcacgga gacctgtgga gtggtaatgt gttctttgac
     2161 cagcaagggc atccagtttt tgtcgaccca gctgtttctt atgggaatcg ggaacaagat
     2221 attgcaatga gccagttatt cggtggtttc cgaccggaat ttttagatgc ttatcaaact
     2281 atatttccat tagaaaaggg atggaaagac cgactgccga tttaccagtt atattatctt
     2341 ttggcacatt tgaatatgtt tggagaaagt tacggttccc aagtagatca attactagaa
     2401 aatttttaag aggagggcgt gtttttggca aaaaaatata ccaaaaagca tgaagtttca
     2461 tattatgagt gtgacatcaa ccaaacgatg acatttcctt cgatgttagg agtagttatc
     2521 aaaacgtctg aagatcaaag cgaccagttg ggaagaggca gtgaattcgt caatagcttt
     2581 ggacttactt gggtcatcac gaattattcc atggagatta atcgtctccc tagagtcggt
     2641 gaagtaatct cagtaacgac tcaagccatg gagtacaata agtttttctg ttatcggaac
     2701 ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa tagaatctgt tttcgtattg
     2761 atggattttg tgaatcgaaa aatgagcagt gttaatgaag agatcattgc tccttttgag
     2821 agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg aaaagattga ttcgggcttc
     2881 atgttgccat atcgtgttcg tttt
//
LOCUS       DACEZN010000229         2886 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEZN010000229 DACEZN010000000
VERSION     DACEZN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.232"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            67..1659
                     /locus_tag="IXH60_002733"
     CDS             67..1659
                     /locus_tag="IXH60_002733"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1991613.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            1652..>2886
                     /locus_tag="IXH60_002734"
     CDS             1652..>2886
                     /locus_tag="IXH60_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1991614.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQK"
BASE COUNT         1045 a          462 c          590 g          789 t
ORIGIN      
        1 aaaagggagt atgctaaaat aagagggtat atagcaactt taaataagag ataggagtgt
       61 atctttttgg gtgcagaatt aaatcaaaaa ttatttagtg cggcagataa tctccgtagc
      121 aaaatggatg cgtcagaata taaaaattat ttattgggac tgattttcta taaatattta
      181 tccgaccgtt tgttggaaca agtggttctt ttagcagatg aatccttaga ggaatatgat
      241 acggtaagca aacaaacgat gttgtatcgc gaattacttt cagatgaaga atcaaaagaa
      301 gatctgattg caacaatcgt tgatattttg gggtatgcga tttcgccaga gtatcttttc
      361 aacgtcttag cggatcaagc aaaacaagca acgttccaat tgaatgatct aaacaaagcc
      421 tttgtgcaat tggcatctac ctacaatcaa tttaatgggt tatttgatga tgtggatctc
      481 caatcaaaaa aactaggaac agatgagcaa caacgaaatg tgacgattac cgaagtcatt
      541 aagaaattga atgacgtgga agttttaggt cacgatgggg acgtgatcgg ggatgcttat
      601 gaatttctga tcagtcaatt tgcttcagaa gcagggaaga aagccggaga attttacacg
      661 cctcatatgg tttctgatat gatggcacaa atcgtgacgt tggatcaaaa agaacgacga
      721 ttctttagtg tttttgatcc aaccatgggt tcaggttcat tgatgttaaa tgtgcgtaac
      781 tacttaactc acccagataa cgttaaatat catgggcaag aattaaatac aacgacatac
      841 aatctagcaa aaatgaactt gattttacat ggggtagatg ccgaagaaat gaatctccgc
      901 aatggggata cgttgaataa ggattggcca acagatgaac cttacacatt tgatgcagta
      961 gtaatgaacc caccttattc ggctaattgg tcagcagata cgaccttctt agacgattct
     1021 cggtttaatc gttatggaaa attagcgcca aaatccaaag cagattttgc gtttcttctt
     1081 catggtttct atcatttgaa ggaaacaggg actatggcca tcgtcttgcc acacggagtt
     1141 ttattccgtg gcgctgcaga aggggtcatt cgtcaaaaat tactagaaga cggcagtatt
     1201 tatgctgtga tcggtatgcc tgcgaacttg ttcttcggga catcgattcc aacgacggtg
     1261 attgtattga aaaagaaccg ccaaactcga gacgtattat tcattgacgc tagtcgagaa
     1321 tttgtcaaag gaaaaaatca gaataaatta tcggaagaaa acattcaaaa aatcctcgaa
     1381 acctatgctg aaagaaaaga tgtcgagaaa tacgctcatt tggcaaactt tgatgaaatc
     1441 aaagaaaatg actataactt aaatattcca cgctatgtag atacgtttga agaagaagaa
     1501 ccaattgata tggttcatgt cggaaatgac atcaagaaga tacgacaaga acagcaagtg
     1561 ctagaaaaag aacttttaga agctatctct tccctgcaaa caacacctga aaatgaagcg
     1621 tggttacaag gtgcgctgga ggtgttcaaa catgagtaat gatatacaac cagaaattcg
     1681 tttcccagga tttacggaag cttgggaaca gcgtaagttt gaatgcttac ttgataaaaa
     1741 agatggagta aggaggggac catttgggag tgccttaaag aaagaattct ttgtatctaa
     1801 tagcaatttt gttgtctatg aacaacaaaa tgctatttat gataattacg aaacaagata
     1861 taaaattact gaaaaaaaat ataatgaatt aatcaaattt aaattagaac caggcgattt
     1921 tatcatgagt ggcgctggaa caattgggag aatttcaaga gtaccaaagc aaataaaacc
     1981 gggtgtattc aatcaggctt tgattagact tagaataaat aaagaaatca cagattcaga
     2041 atattttatt caatttattc gtgcagattt tatgcaaaga aaactcacag gagctaatcc
     2101 aggttcagca attacaaatc tcgtaccaat gtcagaagtg aaaaaatgga tagtacaatt
     2161 tccaatatta gaagaacaaa agaaaattgg gaacttcttc aaacaactgg acgacactat
     2221 cgctcttcaa caacgtaagc tagatttatt aaaggaaacg aaaaaaggat tcttacaaaa
     2281 aatgttccct aaaaacggag caaaagtgcc agaaattcgg tttcctggat ttacggaaga
     2341 ttgggaacag cgtaagttca aagagtttag taagaagact ggaaaaaaga acacgaaaga
     2401 tttagacttc cctgcctatt cggtaagtaa taaagcaggt ttaatttctc aaacagaaca
     2461 gtttgatggt agtaggttgg atgatttaga aaaaacaaat tataaattcg ttgaaccaaa
     2521 tgaattcgcc tataatccag caagagtaaa cgtaggatca attgcattta acaatcttgg
     2581 tatgacggtc attgttagct ctctttatgt agttgtgaaa atgagcgagg acttagataa
     2641 tgagttcata cttcagttta tcaagtcacc aacatttatc aaagaggtca agcgcaacac
     2701 tgaaggaagc gtacgagaat acttattcta tgagaatttc gctaatatca agtttccttt
     2761 tactaggaat aaagaggaac aacaaaaaat cggcgctttc ttcaaacaac tcgacgacac
     2821 gatcgctctt catcaacgta agttagattt gctgaaggaa acgaaaaaag gcttcttaca
     2881 aaagat
//
LOCUS       DACEZN010000230         2886 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEZN010000230 DACEZN010000000
VERSION     DACEZN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.233"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(195..521)
                     /locus_tag="IXH60_002735"
     CDS             complement(195..521)
                     /locus_tag="IXH60_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991615.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(533..733)
                     /locus_tag="IXH60_002736"
     CDS             complement(533..733)
                     /locus_tag="IXH60_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1991616.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            808..1458
                     /locus_tag="IXH60_002737"
     CDS             808..1458
                     /locus_tag="IXH60_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1991617.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1513..2679
                     /locus_tag="IXH60_002738"
     CDS             1513..2679
                     /locus_tag="IXH60_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1991618.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT         1014 a          491 c          419 g          962 t
ORIGIN      
        1 gccttgcttt tcaactaagc tttcaacttc tttttcattc attgtcacac ctgcttctga
       61 ttttatttgc tataatagat tcaaataaag ttttctcaga actttattat tgagtgttcc
      121 cccgatctgc aaactgacgg gaacactctt tttaattttt tctacttcat caaatttaaa
      181 aaacgaggtg ttttttaaat ttgatgactc aatatccatt ttttgcattc ttcttttgaa
      241 aacaactcca ttttttctaa taaaataact ggcaatccca ttcttttaaa tttcaaaaaa
      301 gttgtatatg atacattcaa atatttagcc atctcttttc cttttagaaa tggtctttct
      361 aatccggtat caattcttgc ttgatcaata gcctcttttg tcatttcaaa aacataattt
      421 ttcaattgat tcgtttgttg tttatctaac attactgtaa ttcctgcatc cattattttt
      481 ctcctctcat tttctatttc ttcaatcagc caactcgcca agttgtattt atttagattt
      541 tcaaatcatt aagtaaccaa tttaccaact tttgataagt agatgctcta atactttctt
      601 tgctcccatt ttcgattttg acatatgttt tccgatttat acctatcaat tttgctgcat
      661 cgtttttagt aagtataaga tcggctctat gtcttcttaa gcttctaatg aattgatcat
      721 taagagttat cattcagcgc ctcctttcaa attattcact ttgtggtttt tatcttaata
      781 agcacatttt aaaccacaaa acgagtaata tcaattaaaa tattactcaa tttgtggttt
      841 tcatgctata atagtttgga atttactata gggggcgtta ctttgaatag aataaaattt
      901 tatattgatt ccagtaaaaa gaccattaaa caaattagta cagaaactgg tattaactat
      961 acaactctta cgaactatta ccgtggtgag cgaaatccta gaaatactga aacatggaat
     1021 atccttgctg attatttcca tgttcctgta agttatttga tgggattaga cgaaacaaat
     1081 tccatagaac aacctaatct tgatagaata aaaactgctc gtttagaaaa aaaggacaaa
     1141 atagtcaaga atattgccag tcttgactca gattatttta ctgaattatc taatctatac
     1201 ttttccatga cttctttact tgaagtcaaa cttcaagaag atgactccga ctttcttttt
     1261 ttaaaaagta ttctagagga ctcctttgaa tacacgattc tatttgccaa acaaaagaag
     1321 tttattaata aagctttaaa cttaaatatc gatgaaagaa aagagttaga atctgagtta
     1381 tttggcaact taacccaagt acaggttaac aatctaaaaa gagagataac agctcgatta
     1441 atatcaaaac ttcaataaaa ctaattattc aatcagccaa ctcgccaagt tatcagattg
     1501 gagagattag ttatgtcaat acaaaagtac acaacaaaaa gcggtgcacg atatagtgtc
     1561 cgcttgtatg ttcgtaaaga tgaaaatgga aagcaagaat actatgtaaa aaaagggttt
     1621 aaaactgaaa aagaagccaa gttacacgaa gcaagaaaaa aggtcgagat tgaagatacc
     1681 ggctatcata agccttctaa tgacttgttt ataacagtgt atgaagaatg gttgccttct
     1741 tataggaatc gtgtacaaga aacgacatat caacgtacaa aagacctttt ccgcttgcat
     1801 atactaccaa aattcgggaa taagtccatt tctaagatta ctcctgttca ctgtcagaat
     1861 gcagtcaatg aatgggcttt aaaattcaag aatatcaaac agcttaagtc ttacagctct
     1921 caaatatttg agtatgccat ttttgcagag ctattacacc gaaatcctat ggcaaataca
     1981 aatctaccaa agcgggaaaa gaaagaaaat gaaaattact tttcattaga agaactaaaa
     2041 gctttttggg atattctcaa taaagaagag cctttaaaac atattttaat cttccagctt
     2101 ttgattacta ctggtatacg taaaggagag ctagctgctc ttcattggtc tgacatttat
     2161 tttgaagaac agctaatgta tgttagaaag agctatgcaa ctattcgaaa tgaagagaag
     2221 aatgcaagaa gaaaaactgt caggattcaa aaaaatacaa aaaatatatc ttccgaacga
     2281 atattaccaa ttgatgcaca aactattgaa atgttaaaaa tctggaaaaa agaacaagct
     2341 cgagagcttt tgcagtttgg cataaataca aatacaaaaa atcaacttat ctttacctat
     2401 gtaaatgcag acggtgacat taatcaacct ttgcatgcgg actattcaaa taatatattg
     2461 aaacgtttag agaagaaata taaatttaag cacgttacta ttcatggatt ccggcacact
     2521 cacgctactc ttctactaga aggtggagca agtatcaaag aaacacaaga tcgattgggt
     2581 cataaaaatg ctgaaactac ccttaacact tattctcatg ttactgaaaa agctcaacga
     2641 aatgcgttag aaaaattcat tgcttacact ggtttataat attgtcatta cctttctcat
     2701 taccagaacg aaaaaaagac accttctcgc aatgagaaag tgtctgaaat cgttggtata
     2761 acaacgatta acgttttgaa aactgtgaag ctttacgtgc tttcttaaga cctggttttt
     2821 tcttcgtatc attgaataag tttgaaaaac cttgatttat aaaggttttc ccgtaaatat
     2881 atttta
//
LOCUS       DACEZN010000231         2886 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEZN010000231 DACEZN010000000
VERSION     DACEZN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.234"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(96..827)
                     /locus_tag="IXH60_002739"
     CDS             complement(96..827)
                     /locus_tag="IXH60_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1991619.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(996..1475)
                     /gene="greA"
                     /locus_tag="IXH60_002740"
     CDS             complement(996..1475)
                     /gene="greA"
                     /locus_tag="IXH60_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1991620.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(1611..2786)
                     /gene="mltG"
                     /locus_tag="IXH60_002741"
     CDS             complement(1611..2786)
                     /gene="mltG"
                     /locus_tag="IXH60_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1991621.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT          844 a          591 c          441 g         1010 t
ORIGIN      
        1 gctggcataa aaataagtga aaagtgaaaa tagaataata aggaaagccg caatccccga
       61 ataaacagct agcatcgctc tggatatttt tcccatcata ttcgtatcac ctcgacatct
      121 ccaatcaagg tattagtaat aatcttcaat ctacgagggt tttcatcata atcactgctg
      181 tagattttga tttgttcatt tttcaaagaa aattgctctt cttcaaacaa gacatttcct
      241 actaatgtcg catgctctaa tcggatggct acgcccaaag gtactaagat ccgtgttctg
      301 cctatccctt ttcttacaat cacaatatta tcatcttttg gcaaaagcgt attgcccaaa
      361 tctatgatag tatcaccaga aatgacggcg atgttgatat cgtcccattc atagacgtcg
      421 tttccaatac gttggttacc gaataattgc tgtcttttcc gctcattatt atgtgttttt
      481 aattgctctg tttggatcat catgatctgt ttctttcgcc aaaaagcatt tttggttaaa
      541 tctatccctg aaatctctac acctttcaga ccaataaata aaacggcaaa tacagccatc
      601 atccataaag caggactgtt gactaagcta aaaaaaatga ccaaacttcc taatatcaat
      661 tgaaaatttt gaaatttagt acgaggatgt ttgcgcatag ctagatatat attgaaaata
      721 ccgaatatca ctaaaagcaa aagtccagta ttgtttacga tttgccaaac agcaaataac
      781 agcagtagtg cttcaactac cacaaaaaaa cgccaagaac tattcatcgc tctttctccc
      841 tcctgacaca ttctcatttc atcataccta tttttttaac tgttggctat aggactttag
      901 atgtaagatc gtttgttgaa atctaaaata cacaaagaga aaaagatcta gagtgtatca
      961 tcagtaagat gaactctcta gatctttcag tcagactacg cttgttctac tttgacgatt
     1021 ttaactaaca tatcgccacc tggagtagca atagttactt catcattttt acgtttgcca
     1081 atcaatgctt gcgcaatagg agaatcattg gaaattttgc cagaaaacgg atctgcttca
     1141 gcgcttccga caatcgtgta ctcttcttct tctccatctg gtagttcgat gaatgtaact
     1201 gttttaccaa ttgatacctc atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa
     1261 cgaatcatat tttcaattgt tgtgattctt ccttcaacaa atgcttgttc atctttcgct
     1321 gattcatatt cagagttttc ggaaagatct ccgaaaccgc gggcaatttt gattcgttca
     1381 atgatttctc cgcgtttgac tgtttttagt tcttctaatt ctttttctaa tttttcttta
     1441 ccttctagag tcattggata aactttctct accatgtgta acgtctcctt ttttcgataa
     1501 aataccgcaa aaggtaatct tttgaggaat atagtccaaa aaagattacc acgcttttct
     1561 tcaaatgtaa atgtttttct aaaaatgatc aatgtctaga gcctgttttt ctaagaatta
     1621 tttacatatt tttccactaa ttccatatgt tcttcataag ttttagcgaa ataaacattt
     1681 ccagtgctcg tatctgctac gaaataataa tagtcatttt ctgctggttc taatactgcc
     1741 ttgattgcct cttcgcttgg attgtcaaaa ggtccaggtc catagccggt attggtatac
     1801 aaattatatg gtgaatctac agctgtatct tcatacgtca ctaattcttt gtgttcacct
     1861 aatgcgtata aaacagagat atctgactga agtggcatat ttgctttgat tcggttaaag
     1921 aagacttgtg caatgttttt acgatcattt tcttttacgc cttctttttc aactagagaa
     1981 gctagagtta aaacttcttg tacagtcaaa tttttctgct tgatagctga ataatagttt
     2041 tgcatgaccg tattcgtctt gttcaccatt tcaatcacta gatccttaag cgaagtactc
     2101 gtgtaataat catatgtggc tgggaataag taaccttcca aacggtattt tgtgttctca
     2161 gctttagatg cactttccaa cagttctggg aatttttggt gcaactcgtt aaagaaagtc
     2221 tcatcattca ctaaatctaa aaattctttc ttgtctttac cagttacctt cgctacccgt
     2281 tcagcaatct gatcgatatc gtacccttct ggaatggcaa ttttcccatc tgcaactttt
     2341 gttggctcag aagtaccgcc ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta
     2401 ggcgctaact gataatagcc agcttgaaag ccagttaaat ttttaaattt tgtataataa
     2461 ttgaaaacaa tcccgctttt aataatattg tccttctcca aaatctcacc aatctgcttg
     2521 ttagaagagc cactcgggat ctcgacttgg actaattggt catctgattt atctaaaggc
     2581 ttcaacccgc tatctacata acgatacacg gtaaatccaa gaaatccgcc aataatcaat
     2641 aatgtaagag caatcactaa aatgatcttc ctcacaatct tatcttcttt tttacgcaga
     2701 tttctccgct tgtcgattat atctgctgta gctgccttac tggcttcttt tttatcttta
     2761 cttgggtgtt gtttgtttcc tgacaaaaca actcctcctt actatcaagt tcgtaacttt
     2821 attcattata catgatattt gaattggcgt gaatgatttt atttaaatat tctatcaaaa
     2881 atgtaa
//
LOCUS       DACEZN010000232         2866 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEZN010000232 DACEZN010000000
VERSION     DACEZN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.235"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..2860)
                     /locus_tag="IXH60_002742"
     rRNA            complement(<1..2860)
                     /locus_tag="IXH60_002742"
                     /product="23S ribosomal RNA"
BASE COUNT          603 a          866 c          591 g          806 t
ORIGIN      
        1 actcctgacc tatctacctg atcatctctc agggatctta ctttcttaaa gaaatgggaa
       61 atctcatctt gaggtgggct tcacacttag atgctttcag cgtttatccc ttccctacat
      121 agctacccag caatgccctt ggcagaacaa ctggtacacc agcggtaagt ccatcccggt
      181 cctctcgtac taaggacagc tcctctcaaa tttccaacgc ccgcgacgga tagggaccga
      241 actgtctcac gacgttctga acccagctcg cgtgccgctt taatgggcga acagcccaac
      301 ccttgggacc gactacagcc ccaggatgcg acgagccgac atcgaggtgc caaacctccc
      361 cgtcgatgtg gactcttggg ggagataagc ctgttatccc cagggtagct tttatccgtt
      421 gagcgatggc ccttccatgc ggaaccaccg gatcactaag cccgactttc gtccctgctc
      481 gacttgttgg tctcgcagtc aagctccctt ctgcctttac actcttcgaa tgatttccaa
      541 ccattctgag ggaacctttg ggcgcctccg ttacctttta ggaggcgacc gccccagtca
      601 aactgcccat ctgacactgt ctcccaccac gattagtggt gcgggttaga gtggccataa
      661 cgcaggggta gtatcccacc agcgcctcca tcgaaactag cgttccgatt tctacggctc
      721 ctacctatcc tgtacatgcg gtacagacac tcaatatcaa actacagtaa agctccatgg
      781 ggtctttccg tcctgtcgcg ggtaacctgc atcttcacag gtactaaaat ttcaccgagt
      841 ctctcgttga gacagtgccc aaatcgttac gcctttcgtg cgggtcggaa cttacccgac
      901 aaggaatttc gctaccttag gaccgttata gttacggccg ccgtttactg gggcttcaat
      961 tcgtaccttc gcttgcgcta agcactcctc ttaaccttcc agcaccgggc aggcgtcagc
     1021 ccctatactt catcttacga ttttgcagag acctgtgttt ttgataaaca gtcgcttggg
     1081 cctattcact gcggctgatc gtatgatcag caccccttct cccgaagtta cggggtcatt
     1141 ttgccgagtt ccttaacgag agttctctcg ctcaccttag gattctctcc tcgactacct
     1201 gtgtcggttt gcggtacggg ccgctgtttt ctcactagaa gcttttctcg gcagtgtgac
     1261 atcaggaact tcggtactat tatttccctc cccatcacag cttgtcctta agaaaagaag
     1321 catttgactc ctcttcagac ttactgcttg gacagacatt tccgatcgtc tgcattcctt
     1381 agcctcctgc gtccctccat tgctcaaaca aaaacaacgg gtacaggaat atcaacctgt
     1441 tatccatcgc ctacgcctgt cggcctcggc ttaggtcccg actaaccctg ggcggacgag
     1501 ccttccccag gaaaccttag tcattcggtg gacaggattc tcacctgtct ttcgctactc
     1561 ataccggcat tctcacttct aagcgctcca gcagtcctca cgatctgcct tcaacgccct
     1621 tagaacgctc tcctaccaat acacctaaag gtgtactcca cagcttcggt aatatgttta
     1681 gccccggtac attttcggcg cagggtcact cgactagtga gctattacgc actctttaaa
     1741 tggtggctgc ttctaagcca acatcctagt tgtctgtgca accccacatc cttttccact
     1801 taacatatat tttgggacct tagctggtgg tctgggctgt ttccctttcg actatggatc
     1861 ttatcactca cagtctgact cccggatatg aatgaatggc attcggagtt tatctgaatt
     1921 cggtaacccg agatgggccc ctagtccaaa cagtgctcta cctccatcat tctcaattcc
     1981 gaggctagcc ctaaagctat ttcggagaga accagctatc tccaagttcg tttggaattt
     2041 ctccgctacc cacacctcat ccccgcactt ttcaacgtac gtgggttcgg tcctccagtg
     2101 cgttttaccg caccttcaac ctggacatgg gtagatcaca tggtttcggg tctacgacta
     2161 catactcaaa cgccctattc agactcgctt tcgctgcggc tccgtctctt caacttaacc
     2221 tcgcatgcaa tcgtaactcg ccggttcatt ctacaaaagg cacgccatca cccattaacg
     2281 ggctttgact tgttgtaggc acacggtttc aggttctatt tcactcccct tccggggtgc
     2341 ttttcacctt tccctcacgg tactggttca ctatcggtca ctagggagta tttagccttg
     2401 ggagatggtc ctcccggatt ccgacggaat ttctcgtgtt ccgccgtact caggatcctc
     2461 ctaggtgtct tccacatttc gtctacgggg tttttaccct ctttgactga cttttccaag
     2521 tcattcgact atctgaaaga actaccatat tggagtccta caaccccaac aagcaagctt
     2581 gctggtttgg gcttttcccg tttcgctcgc cgctactcag ggaatcgaat tttctttctc
     2641 ttcctgcagg tacttagatg tttcagttct ctgcgtctac ctcgaatgtg ctatgtattc
     2701 acacaatcgt aacatcctat taaagatgtt gggttccccc attcggaaat ctctggatca
     2761 tagcttacgt acagctcccc aaagcatatc ggtgttagtc ccgtccttca tcggctccta
     2821 gtgccaaggc atccaccgtg cgcccttatt cacttaacct tatcaa
//
LOCUS       DACEZN010000233         2866 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEZN010000233 DACEZN010000000
VERSION     DACEZN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.236"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            29..685
                     /locus_tag="IXH60_002743"
     CDS             29..685
                     /locus_tag="IXH60_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1991622.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            685..2061
                     /locus_tag="IXH60_002744"
     CDS             685..2061
                     /locus_tag="IXH60_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1991623.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2347..>2866)
                     /locus_tag="IXH60_002745"
     CDS             complement(2347..>2866)
                     /locus_tag="IXH60_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAQ1991624.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1088 a          384 c          509 g          885 t
ORIGIN      
        1 cttatcaaaa ataagttagg agaaaataat ggatatatta gaaataaaga atgtagcata
       61 tagttatgca aattctaaag aaaaagtttt gtcaggagta aatcaaaaat ttgaacttgg
      121 gaagttttat gcgatagtag gaaagtcagg aacaggaaaa tctacacttc tttccttact
      181 tgctggactt gataaacctc aaacaggaaa aatattgttt aagaatgaag atatacaaaa
      241 taaaggatat agtaatcata gaaaaaataa tatatcttta gtatttcaaa actataattt
      301 aatagattat ttatcaccaa ttgaaaatat tagattagta aataaatcag cagatgaaag
      361 tatcttgttt gaactaggtt tagataaaaa acaaataaaa agaaatgtta tgaaattatc
      421 tggtggacag caacaaagag tagctattgc tagagcattg gtatcagatg ctccaataat
      481 actagctgat gagcctactg gtaatctaga cagtgttact gctggagaaa taattaatat
      541 attaaagaca ttagctaaag atagaaataa atgtgtaata gttgtaacac atagtaagga
      601 agtagcagat tctgcggata tcattttaga actaagtggt aaaaagttga aaaaagtaaa
      661 taaaatgaat ttggaggttg aataatgata aaaaatgcat ttgcttatgt aactagaaaa
      721 agtttaaaat cattaattat catattagtt attttatcta tgtcaacttt aagtctcatt
      781 agtttatcta ttaaagatgc tacggacaga gcttcaaaag aaacatttgc taatataaca
      841 aatagttttt ctatggagat aaatagacaa gtaaatccag gaacacctag aggtggggga
      901 aatgtaaaag gtgaagatat taaaaagata tctcaaacag atagtataga ctcctatgta
      961 aaaagaataa acagtgttgc agatttagtt gattatgata ttattgaaac tcaagagact
     1021 cttgcgaatc aatcacctga aagagcaaag aattttaaaa gaacagttat gttaactggg
     1081 gttaacgact caacaaaaga aacaaaattt gtatcagaag catataaatt agtagaagga
     1141 aagcatttag aaaatgaaga taaaaataaa atcttaatgc ataaagattt agctgaaaaa
     1201 aataacctta aagttggaga taagataaaa ataaaatcta atttatttga tgctgataat
     1261 gagaaaggtg cagatgaaac agtagaagta gaaattaaag gtctattcga tgggcataat
     1321 agcggtgtag taagtgcagc acaagaacta tacgaaaata cattaatcac tgatattcat
     1381 acagctgcta aagtttatgg taataccgaa gatacagctg tataccaaga tgcaacattc
     1441 tttgtaaaag gtgataagaa tcttgatagt gtaataaaag atcttgggaa attagatata
     1501 aattggagag aatataattt gattaaaagt tcatcaaatt accctgcatt acaacaatct
     1561 atatcaggta tttactcaat ttcaaataaa ttattttttg gctcattaat atttgcagga
     1621 gttgtagttt cattattatt attcttatgg atgaatgcta gaaagaaaga aatagcagta
     1681 ttactatcat taggtatatc aaaattagaa atttttggtc aattcctaat tgagatggta
     1741 tttatatcaa tccccgcatt cgttggttcc tatttcttag ctcaatatac agctgacaag
     1801 cttgggaata atatattgaa taaggttact ggtgatatag ctaaacaaat agctaaacaa
     1861 tctgcatcta gccaattagg tggtggggca gaagctgaag gatttaacaa gacattatca
     1921 agtttagata tcaatgtatt acctaaattc atgatttatg tagttatatt tatgagttta
     1981 gtacttcttg tatcattgat tatttcttca ttcaatatat taagaaggaa tccaaaagaa
     2041 ttattaattg acaataatta aaattttcgg tgctttttag caccaaaaat gagagtgtaa
     2101 tataaaccgt gtaagtaacc atcaatccta ggcgggtggt tgcttcacgg tttttatttt
     2161 tctaaaataa gatcaagagg tgattacacg atggctagaa agaaaagaaa tcctgatgct
     2221 gagaagttag ctgaatccat tttgaatact tatcaacccg aatctgtaga ggatatgcaa
     2281 gatgttttaa aagatgtgtt tggtccctta tttgaaaaaa tgcttcaagg agaattgaat
     2341 aaccatttag ggtatgacgc ccattctaaa gagccaaaga aacatgacaa tcgacgaaac
     2401 ggttatggca ataagacgct taaaacgagc tttggtaaag taaacattga tgttcccaga
     2461 gaccgggagg cttcttttga acctgagttg attcctaata gaaaacgaga tgtctccgac
     2521 atcgcagact gtagacaaaa gctgttccaa actcgtaact cgagcttgga acagcttttt
     2581 atctacttcg tttcgaattt ttgaacaaaa aaataaaggc tccttgcctt tccatagcca
     2641 ggtggctaat ttctttaaat tcattgcagc aaacactagc atcgtatgcg tcgcgacttt
     2701 ttccaagcct cgatacttgg tccaacgcat cccatgcttt tcttttgcat ccgcaaatat
     2761 tcgttcaatc gtttgtttcc gttttttgta aattgattta ttcagatctg ttaggcgaag
     2821 atgttccact tcatccatca gatctcccca aacatgtctc gtgatg
//
LOCUS       DACEZN010000234         2863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEZN010000234 DACEZN010000000
VERSION     DACEZN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.237"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            6..2378
                     /locus_tag="IXH60_002746"
     CDS             6..2378
                     /locus_tag="IXH60_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991625.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..>2863
                     /locus_tag="IXH60_002747"
     CDS             2439..>2863
                     /locus_tag="IXH60_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991626.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT         1144 a          456 c          621 g          642 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg cttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaattga gaagatgttt gaacaccaga
      121 gaaagaccaa cggtcagcca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagaacgttt agaaaacaaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggctataac aagtacggcg
      301 gcctggatat gacaatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gccgctacga tccgaaaata ccaacggaaa ttggaaacat
      421 tagaacagct gaaagaaaga tcagaaaaag gaaaagaaaa tcttcttcct gaagtacaag
      481 caattattga cagcggacga gtgacgcaat ggaaaaaaaa tccaaccatt tattttttga
      541 aagactatcg aaaagttgct ttagaactga atgaaaacgg gggatttgag gaatcaaaaa
      601 aataccaagc cacgtcggaa gaggaaaaag cagctgttcg tgaattactt gaagaggctg
      661 cgccaactga ttctttgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttagaattga
      781 tcgatcaact caaaacgttg gataaccaat acgccgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 aaaaggaaca aaaacaaacg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtgaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gctgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac agttaaaacc tgaagactat ccgaaaatct
     1501 ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg acagtcagaa cttcaagctg
     1561 ggattcaagc ggtagccaac aatattggaa tacaaattcg agatatgaca gaaagtgaag
     1621 tctatcaaag agaattagga caagcacggg gggcgtatgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagct acaagtattc
     1741 atgaattagc ccatgcaaga atgcacaaat tttcagaatt agataccgca actaaagaat
     1801 tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac acttcggaga
     1861 aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat gataaggaag
     1921 cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa ccagcagaag
     2041 tgaagcagga gcataaaaat actgtagaaa cggcagagaa taagaatatt gtgcccgttg
     2101 gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg ggtcgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagtg attttaaaag
     2221 gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa agactatctg
     2401 aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgaaaaa aaaatgagtc
     2461 ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat ggaatggctg
     2521 tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt tttgatcgaa
     2581 gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt catagacaaa gagatccaag actctaagca acaatttaaa gcagccacag
     2701 attttattct aaagaatgaa gacaaaacag aacgagtaga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga aggaattatc
     2821 tgaaggaaga acgaaaacta ccagagtggc tgattgatta tgc
//
LOCUS       DACEZN010000235         2777 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEZN010000235 DACEZN010000000
VERSION     DACEZN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.238"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..197
                     /locus_tag="IXH60_002748"
     CDS             <1..197
                     /locus_tag="IXH60_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991627.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IXH60_002749"
     CDS             314..811
                     /locus_tag="IXH60_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1991628.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IXH60_002750"
     CDS             898..1290
                     /locus_tag="IXH60_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991629.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2777
                     /locus_tag="IXH60_002751"
     CDS             1274..>2777
                     /locus_tag="IXH60_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1991630.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPK"
BASE COUNT          839 a          517 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaag
//
LOCUS       DACEZN010000236         2746 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEZN010000236 DACEZN010000000
VERSION     DACEZN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.239"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(56..349)
                     /locus_tag="IXH60_002752"
     CDS             complement(56..349)
                     /locus_tag="IXH60_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1991631.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(377..1042)
                     /locus_tag="IXH60_002753"
     CDS             complement(377..1042)
                     /locus_tag="IXH60_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1991632.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1182..1814)
                     /locus_tag="IXH60_002754"
     CDS             complement(1182..1814)
                     /locus_tag="IXH60_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1991633.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1821..>2746)
                     /locus_tag="IXH60_002755"
     CDS             complement(1821..>2746)
                     /locus_tag="IXH60_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1991634.1"
                     /translation="YVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSG
                     NYESKLLLLPIPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEE
                     ILELERHRLARELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINAS
                     QSEMRALLLHLRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLF
                     RIVQELLSNTLRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKE
                     RVAGIGGTVKIISFKGQGTSVEIKVPLMKEA"
BASE COUNT          761 a          596 c          416 g          973 t
ORIGIN      
        1 atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt
       61 gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca agtaaatcat
      121 atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca tcaatttcca
      181 ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa
      241 cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa
      301 tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct
      361 actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct
      421 ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga
      481 taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat
      541 ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag
      601 aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa ttatgcgcaa
      661 tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc ttttcaagta
      721 ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc atttccttta
      781 ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca attacatgat
      841 cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt gccacaacag
      901 catgagtggc tatattcata tattcattga cccgatcttc attactatca atagccagta
      961 cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt ccaagaccta
     1021 caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc tcagtataac
     1081 aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata
     1141 gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt
     1201 ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc caatatattg
     1261 gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat ttcctgatta
     1321 ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag ttcttcatgt
     1381 aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac ttctggttct
     1441 aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg tgcggtagag
     1501 gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt ttcgtcatca
     1561 ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat tgcttttgtt
     1621 gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc cggtcgaagt
     1681 gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac ttcaatgtct
     1741 tcttgaatag ataaataaga agagacgcct aagcgcacca tttcatggtc atctactaat
     1801 aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat ttctacactt
     1861 gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc tactcgttct
     1921 ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa ccctgttccg
     1981 tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac ctctaattca
     2041 ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg gaacaagtga
     2101 tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga aatctggatt
     2161 ttattttgca attccttcaa taactgctcg ataccttgct ttaaactttt ctcttctaaa
     2221 ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc
     2281 tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt ttctgcttgc
     2341 tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac tgaatcatgc
     2401 agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt ttgtccatct
     2461 acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat cattttttct
     2521 tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga tgcacttggg
     2581 atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg cagcttttct
     2641 tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac aattaatcct
     2701 acacctaagg aaatggctaa caaatggaag ataagcggaa catata
//
LOCUS       DACEZN010000237         2705 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEZN010000237 DACEZN010000000
VERSION     DACEZN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.240"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1225
                     /locus_tag="IXH60_002756"
     CDS             <1..1225
                     /locus_tag="IXH60_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1991635.1"
                     /translation="FFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGGKNTFQAAPD
                     ILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSFQYGLHDKPF
                     FQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETINGYFATANYL
                     DTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSSTWTNYDNAQM
                     QRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDLFSKDHEQLV
                     SFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQLAVSDQINN
                     GDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNGQSTVDLYKT
                     QTYQELHGTTTSSSE"
     gene            1392..>2705
                     /locus_tag="IXH60_002757"
     CDS             1392..>2705
                     /locus_tag="IXH60_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1991636.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTT"
BASE COUNT          892 a          554 c          508 g          751 t
ORIGIN      
        1 ctttttccat caagtaaaag caggaaaaac aagcgatgcg gaaacgctga tggaaaattc
       61 attgtttggc ttgaatcaag gctccttgtt cacacaatta ggcggaaaaa atactttcca
      121 agctgcacct gatattttaa gtcaaacaca aggttacatt agtgcggtat tccatgggaa
      181 cgctggtaca ttctggaatc gaaacgagac atacaagaat ttaggatatg attatttctt
      241 tgattcttct tattatgatg ttaatgatga caattcgttc caatacgggt tgcatgataa
      301 accatttttc caacaatccg tacaatactt ggaacgtctg caacaaccat tttattcaaa
      361 attcattgct gtatctaacc attatcctta ttcaaaattc accaatgacg aagcaggatt
      421 cccgttagcc aatacaaatg atgaaacgat caacggttac tttgcgacag ccaattattt
      481 agataccgcc gtacaagaat tcttcaatta tttgaaagaa agcggcttgt atgataattc
      541 agtcatcgtc ctttacggtg accattacgg tgtctcgaat tcaagaaata aaaatctagc
      601 tgaattagtt ggaaaaacaa gttctacttg gacaaactac gacaatgcac aaatgcagcg
      661 tgtcccttat atgatccata tccctggaca aacacagggc ggaatcaatc acacttacgg
      721 cggacaagtt gatgccttgc ctacattact tcacttacta ggtgtagata caaaaaatta
      781 tattcagtta ggacaagatc tattctcgaa agatcatgaa cagcttgttt ctttccgtga
      841 tggtgatttt gttacaccaa aatatactta ctacggtggt acgatctata gtaacgaaac
      901 tggtgaagct attactgaac ctactgagga agtacagaat gaaattgatt cactgaaaga
      961 aaaagttgat aaacagttag ctgtttctga ccaaatcaat aatggtgatc ttcttcggtt
     1021 ctatacgaat agtggattag acccaattga taccagtgat tatgactaca agaatggctt
     1081 agagaaatta aaagaagctg aaaaatctct tggtgacaag tcaactagtg tctttagtga
     1141 acacaacgga caatcaactg ttgatcttta taaaacgcaa acgtaccaag aacttcacgg
     1201 aacgaccaca agttcttccg aatgataaaa taaatttaaa aggagcagcg acaaaattag
     1261 gatctataat atctagtgtt ggtgacgcct tctgttgcca atactttttc ttttttctaa
     1321 taaacgcaaa cagacacctt gaaatccttt agaataaagt cgacgaaaac cattcaaagg
     1381 agtgtttcaa gatgtcctat tcagagttta ccaaagagtt actagatatt ctagacctaa
     1441 atcttacctt tcatgaagat gcctttcgca aagagcgcat taatgacgaa acatgttttg
     1501 tttttgatgg cacattgacc tatcaaccag aggaatgctt ccattgccac tatcaaaata
     1561 agcaaacgat cattaaatgg ggctggaaaa aagtatccat cttattgaat gatgtcagta
     1621 actacaagac cattgtgcgg attaacaaac agcgcttcaa atgtaaacac tgtggaaaga
     1681 cttttttagc agaagattca gttagcgatc gtcgctgctc cattgcccgc agagtcaaac
     1741 aagccattct tgagctatta agtgagccta tttctatgtc tttgattgca cgtatgaagc
     1801 acatttctcc gaccactgtc attcgaatac ttcgcagcct tcggcctaaa accgtttctt
     1861 tgaaccagcc gttaccagaa gtggtgtgtt tcgatgaatt caaatcggtg aaaaacgtct
     1921 cgggagccat gagctttgtc atgatggacg gcaagaccca tcaactgatt gatattgtgg
     1981 aaaatcggca actcaatcct ttacgcgact atttcttgcg ctaccctaga aaagtccgtg
     2041 aacaagtacg tctagtggtt tcagatttct atacacccta tcgcacattg gtgaaagagt
     2101 gttttccgaa tgcccgaatc gtcgccgatc gctttcatat tagccaacac atcggtcggg
     2161 cctttaccaa ccaccgaatt caagtaatga agacctttaa aaaaggggat cgtcggtcga
     2221 agcacttaaa gaaatactgg agactacttc aaaagaatgc gtgggaattg aatggtcagc
     2281 atcgctactg gcgtccgtca tttcgagatc atctgacaga agcggagata gtcgatcggc
     2341 tattatatta tgatgactcc ttaaagcgag gctacgaagt ctaccaggtt tttctttccg
     2401 ctatcagaag acaagatgtt cctgaattcg tcgcgctcct aaaagaagac tacaaggagt
     2461 tgccagagca ctatcaacca gtgttcacga ccttcaagaa atatcgaaca gaaatcaaac
     2521 gagcgttgcg agttccctat tccaacggcc ctatagaatg tttgaacaac catatcaaag
     2581 tactaaaacg aattgcctat ggctttagaa actttcaaaa ctaccgagaa cgaatctttt
     2641 tatatcgagg aaaatatttc aagaaaacta agaacactac ccaactgact aaagccagaa
     2701 caaca
//
LOCUS       DACEZN010000238         2677 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEZN010000238 DACEZN010000000
VERSION     DACEZN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.241"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..204
                     /locus_tag="IXH60_002758"
     CDS             <1..204
                     /locus_tag="IXH60_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAQ1991637.1"
                     /translation="IKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNV
                     LEIAMLKNINRDYSELEKYLGES"
     gene            216..1043
                     /gene="panB"
                     /locus_tag="IXH60_002759"
     CDS             216..1043
                     /gene="panB"
                     /locus_tag="IXH60_002759"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ1991638.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1058..1906
                     /locus_tag="IXH60_002760"
     CDS             1058..1906
                     /locus_tag="IXH60_002760"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ1991639.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            1916..2311
                     /locus_tag="IXH60_002761"
     CDS             1916..2311
                     /locus_tag="IXH60_002761"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ1991640.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            2513..>2677
                     /locus_tag="IXH60_002762"
     CDS             2513..>2677
                     /locus_tag="IXH60_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1991641.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
BASE COUNT         1054 a          278 c          561 g          784 t
ORIGIN      
        1 ataaaagaaa aaggaattat agaaagtttg acagggccag ttgaacgttg tgatatttca
       61 acaattaaag gtcactgtga agttttatca gatgaagaca gagctttata tatgctatta
      121 tcaaaaaatg tattagagat agctatgtta aaaaatatta atagagatta tagtgaatta
      181 gaaaagtatt taggagagag ttaggagaga aatagatgaa aaatacagca gttacattta
      241 aagaatctaa attaagaaat gaaaaattaa caatgcttac agcttatgac tattcaacag
      301 caaaaataat tgatgaagct gggataaatg gaatattagt aggagattct cttggtatgg
      361 tatgtctagg acatgaagat acactttcag taacaatgga agacatgatt catcatacta
      421 gagcagttac aagaggagct aagaacactt tagttgttgc agatatgcca tttatgtctt
      481 atcaaacttc agtatatgat tcagtagtta atgcaggaag attaataaaa gaaggacgag
      541 ctcaggttgt taaattagaa ggagggatag aagtatgcga taaaatagag gctatagtaa
      601 aggcttctat acctgttatg gcgcatatag gtttaacacc tcaatctgtt aatgcatttg
      661 gaggatttaa ggtacaagga aaggacaagg aagctgctaa agaattaata agagctgcta
      721 aagctgtaga aaaagcagga gcattcgctg tagtgttaga atgtgttcca actaagcttg
      781 cagagttaat ttctaaagag atatcaatac ctacaattgg tattggagca ggtgcagggt
      841 gtgatggaca aattcttgta tatcaagata tgttggggat gtatagtgat tttaccccta
      901 aatttgtgaa gaaatatgct aatttatcag aagagatgaa taaagcattt acaaaatata
      961 tagaggaagt aaaagatgga gtttttccag gtccagaaca tgggtttaaa ataagtgatg
     1021 atgttttaga aaaattatat taaaagggtc aagaaatatg gttatattta aaacagtgaa
     1081 tgatgttaga agtcaagtaa aagagtggaa aaaacaaggc ttaaaggtag gattagttcc
     1141 tactatggga tatttacatg aaggtcatga aagtttaatt agaaaagcat caaaagaaaa
     1201 tgataaagtt gttgtaagta tctttgtaaa tccaactcag tttgggaaaa atgaagattt
     1261 aggtagttat ccaagagact tagaaagaga tatagaagta tgcacaagag gtagagcaac
     1321 agcaatattt aatcctgaag ttgaagagat gtattgtgac aatgcaagta cttttgttaa
     1381 tataactgga ttaacagaag gtttatgtgg agctagtagg ccaatacatt ttagaggggt
     1441 ttgcactgtt gtaagtaaat tatttaacat aataccagca gatagagctt attttggaga
     1501 aaaggatgca caacagttag ctgtaattaa aagaatggtt agggacctta atattgatat
     1561 agacgttgta gggtgtccaa ttataaggga agaggatgga ctagctaaaa gttctagaaa
     1621 cacatactta agtttagagg aaagatcttc agctactatt ttaaataaat ctttaacttt
     1681 agctaaggaa gctttaaata atggagagag agacagctta aaaataattg agataataag
     1741 taaaaatata aatacttata atttggcaaa gattgattat gtagaagttg ttgattcttt
     1801 atcattacaa agagtaaatt atattgaaaa atcagtttta gttgcaatag cagtatttat
     1861 aggaaaaaca agattaatag ataattttac atttgaatta caataggggg ctataatgaa
     1921 agttacaatg ttaaaaggaa aaatacatag agctacagtt gaacaagctg agttagatta
     1981 tgtaggaagt attactgttg atatgaattt attagaagca gcaggaatat atgaatatga
     2041 aaaagtacaa atagttgatg ttaataatgg aaatagattt gaaacttata caatagctgg
     2101 agagagagga acagggatga tttgcttgaa tggagctgca gctagatgtg tatcaactgg
     2161 agataaaata attttaatgg catattgtga attaaatgaa aatgaggtta aggatcatag
     2221 acctaaggtt gtttttgtcg atgataataa taaagttgaa agagtaacat catatgaaaa
     2281 acatggaagg ttatcagata taattcttta ggttttgtaa tgttatttat atatagctag
     2341 actattattg aaatatttat tgattactaa ttggatagat gtcaataaag tagacacaaa
     2401 aagagaagat acagactgta gacaaagtcc attttatgtg aatcggctac actagactag
     2461 acagaaaaaa taaggtgtgt agaataataa aaaacacact ggaggatttt tcatgtcaag
     2521 acgtcaacga agaacctatt caaaagaatt caaacaacaa atcgtcaatc tctatctcgc
     2581 tggtaagcct cgcgcagaaa ttattcgaga gtatgagctt acgccttctt ctttcgataa
     2641 atggatgaag caagcacaat caacgggctc attcaaa
//
LOCUS       DACEZN010000239         2668 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEZN010000239 DACEZN010000000
VERSION     DACEZN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.242"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(23..637)
                     /locus_tag="IXH60_002763"
     CDS             complement(23..637)
                     /locus_tag="IXH60_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1991642.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            951..1373
                     /locus_tag="IXH60_002764"
     CDS             951..1373
                     /locus_tag="IXH60_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991643.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1383..1586
                     /locus_tag="IXH60_002765"
     CDS             1383..1586
                     /locus_tag="IXH60_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991644.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1797..2381
                     /locus_tag="IXH60_002766"
     CDS             1797..2381
                     /locus_tag="IXH60_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1991645.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2550..>2668
                     /locus_tag="IXH60_002767"
     CDS             2550..>2668
                     /locus_tag="IXH60_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1991646.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVV"
BASE COUNT          885 a          413 c          475 g          895 t
ORIGIN      
        1 ataataaaag cagcatgatc aactatttgt cattgaccat gctgtctttt tttattctat
       61 acacggtttg tctcgtaaca ttgtagtctt tagcaatctt agcaatcgca acaccatttt
      121 taagatcttc aacgatgctt ttgtaaacaa ggcgacgttg gggatctttt gcgtctgcac
      181 tgtataattt gggccgccct ttgtagacgc cgtttgcttt tgcgatctta attccttgtg
      241 cctggcgacg tttcgattca gtccgttctt gttcagcgat catggccaaa atttggatga
      301 tcagatcctt aatgaattta tccaggagag gattgccaat tgcttcagcc atgatcggta
      361 aactagtaat gatcagcttc acatttttct tcttcaagta attgactgaa gtgatgatct
      421 cgtcataatt tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga
      481 cgtaatctaa tgcttcttgg aaaacaggtc gatgagtaat cgttgcgcca gattgtttct
      541 cgatgaaaat tctctctgca ccgaattttt tcatttcttc aatctggcgt tgttcatttt
      601 gatcgatcga tgagacacgt atgtatgcaa ctctcaaaac ttttacctac tttctgcaac
      661 tatttaaaat acaccctaat catacacgag gaagccatat ttttcaatgt tataccagtg
      721 tctatttagg gtaaacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca
      781 cttttctttt taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg
      841 attatgacat taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta
      901 agatttatta agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata
      961 agtttatttt atctttaggt attttaacta ttggaattag tgtaactgga actacaattc
     1021 ctacatttgc tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa
     1081 ctcctttaga taggaacgga atagccattg gtaacccaac taacgactta actgttactg
     1141 aaaattattt aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga
     1201 caggaacgat gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt
     1261 ggaaagaaaa aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat
     1321 cagctggaaa caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt
     1381 ttatgccaga atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa
     1441 ttaatttacc gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa
     1501 aaattcaaaa atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc
     1561 aaaaaacata tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct
     1621 ttttttctaa ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct
     1681 ttatttttca aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca
     1741 gttaaactat atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga
     1801 aaataggata cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc
     1861 taaataaatt tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc
     1921 gaccaggatt agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc
     1981 gcttagatcg tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg
     2041 agcgtggtgt gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta
     2101 caggacagct catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc
     2161 tggaacgatc cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc
     2221 ctgaaaaatt gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac
     2281 ctattaagac tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg
     2341 caaaaatgga ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg
     2401 aaaatttttg ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct
     2461 actgataact tattctcaaa tatttagcta tttcttcttt acaacatcga acatatgttt
     2521 gtataatgtg gaagaatgga gtgttattta tgatggatta ttcaaaagag ccagtaaatg
     2581 attacttttt aatcgatatg aaaagctttt acgcaagtgt cgaatgtata gaaagaaatt
     2641 tagatccatt aacaacagaa cttgttgt
//
LOCUS       DACEZN010000240         2640 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEZN010000240 DACEZN010000000
VERSION     DACEZN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.243"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IXH60_002768"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IXH60_002768"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1991647.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          948 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
//
LOCUS       DACEZN010000241         2613 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEZN010000241 DACEZN010000000
VERSION     DACEZN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.244"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(58..1434)
                     /locus_tag="IXH60_002769"
     CDS             complement(58..1434)
                     /locus_tag="IXH60_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1991648.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1562..2608)
                     /locus_tag="IXH60_002770"
     CDS             complement(1562..2608)
                     /locus_tag="IXH60_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1991649.1"
                     /translation="MISSEINFIFEPIGTFFSTLFAPVLIAGFLYYLLNPIVKLLMKA
                     KIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDFIKQVESWLKEVAEL
                     PLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASATVVIITAPFILFYM
                     LKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIECLFVGTFTFLGYMV
                     IGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKALLCCVVVLIVQQLDG
                     NIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAICKTIISYIIKIVKE
                     DKQNENKKKIATTS"
BASE COUNT          817 a          537 c          431 g          828 t
ORIGIN      
        1 gaaaagacgt gttgcttgtt tattcaagca acacgtcttt cataaccaag tttccaatta
       61 tcttcttggc tcgtcatctt cgaattcttt tctgaagtat cttcgttctt cttcaggttc
      121 cgcttctgtt tcttccggat catgaaaaat gacacggatt ttaaggattc gagaaccttc
      181 catttcttca gaaaccagcg taatattatc gacatcaaac gataactttt cgccttcatc
      241 cggaatcatg cctaatgctg taatcaaata accagccatc gtatctacat cactcatatg
      301 cagatcactg tcaaaagctt cattgaactc atcaatcagc atacgtcctt ggatgatata
      361 ttcgtgttca tcgatttttt cataaagatt ctcaacttca tctgattcat catcgatttc
      421 tccgacgatt tcttctaaaa ggtcttctag tgttgctaac ccaacgacgc cgccatattc
      481 atctaataaa atagccatct gattctgcgt ctttttcatt tcatataaca gatcatcaat
      541 aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt ttcttgatat ctaaattatc
      601 aaagccgaat ttatgggcag cttttagtaa attctttgta tgaaggattc cgactacttt
      661 atctttgtct tcgttataaa cagggattct tgaatagttt tcggataaaa cttcattcac
      721 attttcttcc acagtgtcgt tgatatcaat cataaatgca tccgttcgtg ggaccatgac
      781 ttctcgggca actttcgtat caagagaaaa aaccccttgg agcatttcta attcttcatt
      841 attcaatacg ccttcgtttt caagcatata acgcatttca tctctggtca tttttgaatc
      901 ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa cttgtggaag cagaaagcag
      961 ccagacgaag ggcttagcta ctacaccaat caagcgtatc agccctgaag tgaactttgc
     1021 gacttcttct gatttattta aagcaatacg tttagggtac aattctccaa aaacaatcga
     1081 tacataggtc aaaatagcca gcacaatgat atttgcaata ttttttgccg ctgtgccgcc
     1141 acctaatacc ggagctaacc gttccgataa agtattagct agtgaagcac cagacaaaat
     1201 gttgaccaaa gtgattccta cttggattgt cgataggaaa ttatttggat tctgcaatat
     1261 ttttaaaagc ttttgagatt tcacatctcc ttcatcagct ttttgctcga ttcgattctt
     1321 attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa gcattgatca atgtcaaaac
     1381 gatcaataat aaaatctgcg ctaacagcga ctgactctca gggtcagcat tcataatcgc
     1441 attctctcct tataaaatta atttttagtt tacgatagaa aaaaacgtgg cactatagcc
     1501 acgttaaaat ggtatcacaa agcaattttc tttgcaataa aaacaagtgt actaaaacta
     1561 cctacgaggt cgtagcaatt ttcttcttgt tttcattttg cttgtcttct ttgacgattt
     1621 tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa aggaacacct aagaagatac
     1681 caagcaatcc ggcaatattt ccagctacta gaagaacgat aatgatcgtt aatggatgga
     1741 ttttcagtga tttcccaatt acattaggat aaatgatatt cccatccaac tgttggacga
     1801 ttagcacaac cacacaacaa agtaatgctt ttaccggatc attgaagact gttactagaa
     1861 atgcaggtgc tagtcctaga taaggtccta aatatggaat taggtttgtc aatcccgcaa
     1921 tgaccccaaa taagaaagca taatccacac caattaccat atatcctaaa aatgtgaagg
     1981 taccaacgaa taaacattcg attgcttgtc cgctaatgta attagccaat gttttattca
     2041 gttgccccaa taactccaca atttggtttc ttcttttttc tgggaaaaac tgttggatgt
     2101 ttggcactag tttttctccg tctttcaaca tatagaacaa gatgaatggt gctgtaatga
     2161 ttactacggt tgccgaagcg atcgttccaa cgatcgaacc taaactattg gatactccac
     2221 taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa ttgctcaaaa tatttattga
     2281 tatctacttc cttgaacaaa ggcaattcgg ctacctcttt taaccagctt tctacctgtt
     2341 tgatgaaatc aggcatatta gatgccaatg aggaaatttg ctgtactaga cttgggatga
     2401 cactaagtaa aatccaaata attgctgcaa ctaaaaggag cagcacaatt gcgacagccc
     2461 atattctttt gatttttgct ttcatcaaca gcttgacaat aggattcaat aaatagtaaa
     2521 gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa aaatgtcccg atcggttcga
     2581 aaataaagtt tatttctgat gaaatcatga tca
//
LOCUS       DACEZN010000242         2483 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEZN010000242 DACEZN010000000
VERSION     DACEZN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.246"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(1014..1304)
                     /locus_tag="IXH60_002771"
     CDS             complement(1014..1304)
                     /locus_tag="IXH60_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991650.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IXH60_002772"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IXH60_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACEZN010000243         2417 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEZN010000243 DACEZN010000000
VERSION     DACEZN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.247"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            345..1925
                     /locus_tag="IXH60_002773"
     CDS             345..1925
                     /locus_tag="IXH60_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1991651.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2123..2347
                     /locus_tag="IXH60_002774"
     CDS             2123..2347
                     /locus_tag="IXH60_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1991652.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          873 a          389 c          509 g          646 t
ORIGIN      
        1 cttttagtca gctgacaaaa aaatatttga ctcttatggt ttggtggctt tttcattcgc
       61 tgaatccatg ctatactgat tcagttgtag tagttgacaa aggttgtgaa aaagctatcc
      121 tgcattaagt aataagagca gccaagcaaa aacagctttt cgtatttttg gcttgggtgg
      181 gcttattgct gataggctgt ctctagaacg ccgtttactc ggaaaaagag actgctacac
      241 ggcttttgtc acagccctat atgaaattgt ctttctttaa gagatcggac aatctgtgcc
      301 tagagaaaat ataaataatt gaatggaaga aatggagtta ggttatgaat aatccaaaat
      361 taaagcaaca agtggacaat cgcagaactt ttgcgatcat ttcccatccg gatgctggga
      421 aaacaacgat tacagaacaa ttactattgt ttggtggggc tatccgtcaa gccgggaccg
      481 taaaaggcaa aaaaacaggt aattttgcca aatcagactg gatggaaatc gagaaacaac
      541 gtgggatttc tgttactagt tctgttatgc agtttgatta tcaagggaaa cgagtaaata
      601 tcttggatac accaggacat gaagattttt ctgaagatac ttatcgtact ttgatggccg
      661 tggatagtgc ggtcatggta atcgatagtg caaaagggat cgaagcacag acaaagaaat
      721 tattccaagt tgtaaaaaaa cgtggcatcc cgatatttac ttttatcaac aaactagatc
      781 gtgatggtcg tgaaccattg gaattgttag aagaattaga agagctatta gatatcgaat
      841 cttatccgat gaattggcca atcggtatgg gtaaagggct agaaggtctt tatgatatct
      901 accataatcg tgtagagttt tatcgtccgg aaaattatca ggatgaacga ttagtagaat
      961 tggatgaaga tggactgctt cccgaaaatc atcctctaac tgaaaattcc ttatatgaac
     1021 aagtcttaga agaagtagaa ctaatcaaag aggctggaga tgcatttaat caagaaaaaa
     1081 ttgctcgtgg cgatcagaca ccagtatttt ttggttctgc attgactaac ttcggtgtac
     1141 agactttttt agagactttc gttgaattgg caccagctcc ttatggacac aaaacacaag
     1201 atgatcagat ggtaagtcct tatgaggaag aattttcagg tttcgtattc aagatccaag
     1261 caaatatgaa tccagcccat cgtgaccgaa tcgcttttgt ccgaatctgt tctggaacat
     1321 tcgaacgagg aatggatgtc tggttggaac gtacaaataa aaaactgaaa ttaagcaatg
     1381 tcacacaatt tatggcagat tcgagagaaa acgtcgaaaa agcagtagca ggcgatatca
     1441 tcggtgtata tgatacaggc aattatcaga ttggggatac attgttcgaa ggaaaactaa
     1501 aagttgctta tgaagaactc ccttcattta ctccagagct gtttatgaaa gtgactgcaa
     1561 aaaatgtcat gaaacaaaaa tccttccaca aaggaatcta tcaattagtt caagaaggag
     1621 cgattcagct ttataaaaca tatctaacgg aagaatatat cattggagct gttggtcagc
     1681 tacaattcga agtattccaa tatcgtatgt taaatgagta taacgccgaa gtaatcatgt
     1741 ctccaatggg acacaaaatc gctcgctgga tcgatccaga agatttggat gagaagatga
     1801 gttcaagtcg caatatcttg gctcgtgacc gtttcgacca accgttgttc ttattcgaga
     1861 accaatttgc agaacgctgg tttgcggata aatatcccaa tgtaaaatta aaaagtttga
     1921 tgtaaagaag tcttgacaca agctgccctg catcaagtaa taagaatagc caaacaaaaa
     1981 cagcctctca tattttgttt agagccgtga cggattttcc gtgacggctc tttttactat
     2041 attaaaatca tagaaatgtc taatcaaaaa aatataaaaa ttattttgcg tacatatgat
     2101 taaagaaagg agagtgtgac acatggaaac agaatggcaa agacgtgaaa gaaaaaaaca
     2161 gaaaaaagaa gagatgttac gattatgtcg attattagat gtaaaagact gggacaaaaa
     2221 cgagcaaatt gcacaaaaag tacgatcaat catccataaa gaggaaaaag ttaaaaataa
     2281 agacacgcgt agaactgaaa atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa
     2341 aagctagaaa gaaaaagagg ctgagacaga agcgtttaac tccaagaaat aagctggaat
     2401 tcacaaaaat ttgaaaa
//
LOCUS       DACEZN010000244         2399 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEZN010000244 DACEZN010000000
VERSION     DACEZN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.248"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            206..2068
                     /locus_tag="IXH60_002775"
     CDS             206..2068
                     /locus_tag="IXH60_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1991653.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2399
                     /locus_tag="IXH60_002776"
     CDS             2068..>2399
                     /locus_tag="IXH60_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1991654.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DACEZN010000245         2367 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEZN010000245 DACEZN010000000
VERSION     DACEZN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.249"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..108)
                     /locus_tag="IXH60_002777"
     CDS             complement(<1..108)
                     /locus_tag="IXH60_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1991655.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAY"
     gene            complement(101..874)
                     /locus_tag="IXH60_002778"
     CDS             complement(101..874)
                     /locus_tag="IXH60_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1991656.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(1030..2064)
                     /locus_tag="IXH60_002779"
     CDS             complement(1030..2064)
                     /locus_tag="IXH60_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1991657.1"
                     /translation="MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNF
                     CKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPI
                     SHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIF
                     DFNALKKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDS
                     DSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINL
                     FLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYEL
                     IYTIFEQTIF"
BASE COUNT          782 a          450 c          308 g          827 t
ORIGIN      
        1 atacgcataa cccaaccctt ccattttttc ataatttaat gaagttgcac cattgagaat
       61 ccaccggaaa attacttgtt tgtcgatttt tgtcagtttc ttattcatct tcgtcaaaac
      121 ctcctgttgc agctggtgct acttgagtat tatttgatct atttttatat acgattagtg
      181 cgattgcagc gccaagtaac gaaatcccta aaattggtat ttggaaataa actgccaaaa
      241 cgaaaccaac taacaaatag gcaaagtaat cttttgtagg taaatattta agtagcattc
      301 ctatcccaac agcaggtaat agtccccctg ctacctttaa accaccggta acccattcag
      361 gaatataatt gattgcactt cccactaacg ttgggcctaa tatcacagtt aacaaaacag
      421 gaattccaga tacaaagctt gtattaattg atgccaaata ttgcatccac tctaattttt
      481 gataatcttc tttttgtgcg aatttttctg ccttttgttg aaaaaaaatg tttgttgacc
      541 actttagaac atctagttgt acaattaaca ttgctaccgg tattgctaaa gttattccta
      601 tagaagcatc ctggttagtt gagattgcta aataagtacc gatcaatgct gctgtttgat
      661 aatccggtac tgatgcacca ccaaaactag taatacctag tgtcattaat tgcaaagtcc
      721 caccgatata aagtccagtt tgaatatctc ccataataag tccggtcaag aaacctgcaa
      781 cgacaggttg ggaggttccg attttgggcc cttccttatc ccagttaatg aaaaatccat
      841 acaacacaac taaaaatgtt tgccaaaacg ccattttgtt ctctcctttc ttcttatact
      901 tttatataag caattactgt gccaactaac attatccaat aaagcgggaa aatacagtaa
      961 atattaatta aatgagcgtc taaatatact ttggacaccc tttcattttt ttagacgcta
     1021 gtatgctatt tagaaaatag tctgctcaaa aattgtatag attaactcat actctccttt
     1081 gggtatttca atacagtaaa tatctttgat ttctgaaaaa aaatcttttg atacatttaa
     1141 aaaagtatca aatagttgcg ctccctcttt caaatgttca tttgttattc catcaccaat
     1201 cataattcgt tcaatcattc cagaaagatg tagaaataga ttaattctca gaaaacttgg
     1261 gattttgaca tcgtaatatt tttcatacaa atatagtact ttttctatct cattgaatac
     1321 ttttttggaa tctaaaaagc gaagcttagt actagcgcct tcaatagtaa agagtttaac
     1381 taattcattt aagcagaact cacagctatc cgagtccata tacttattta atttttctaa
     1441 ggtttgattc ccattaatta gattttctaa atttaaacaa tcgattcctt gaatgtaaaa
     1501 ttcagtggtt gaaagaacac aaattgtatt cttaaaaatc atagtatctt tttcctttgc
     1561 tattttcttt aatgcgttaa aatcaaaaat caaaatctcc acttgattat cgagataccg
     1621 ttttaaaata tccttaactt tttcagaaat cccctcacca gagagacaag atactattac
     1681 atttttcttt tgtgataatc ctttataata ctgaacttta acttgaaacg aatcaaaatc
     1741 catctggtaa aaatgactaa ttggcttttt ttggcaaact tgaatcccac attcaatcgc
     1801 taaagctgta gaaacattgt ttaaaattac caaatcacct attacattgg attcaatttt
     1861 ttcatacatt tgttccaatg atcccatatc aactagcaga atgagtccat tcgtcgtatc
     1921 tatactttta caaaaattgt tgattaggtt aattaagtca ctagtctcac cttcaattgg
     1981 catatcgaaa gcctcataaa catatgtatt cagcaactta tttgtttccc ttgcgatcgc
     2041 agaagctgtt attttaccgt gcataactat cagacaatgg gtttctggta ttttcacata
     2101 gaacttcttt acaagaaaaa gtatccattt caacaaaagt tcttgtttac tttcttcgac
     2161 ttctaaacta ctccaaatct catttgccaa taatttttgt ttatcatttt tcatacgaaa
     2221 ttttctgaac ttagacagca aaaaatttct tgatgtttct tcaattacat caataaacat
     2281 tataaataga ctgatatccg ccaagaattc atctaataaa catagacctg tcgaatcaat
     2341 aattggtgat attttattaa caacata
//
LOCUS       DACEZN010000246         2320 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEZN010000246 DACEZN010000000
VERSION     DACEZN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.250"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..408)
                     /locus_tag="IXH60_002780"
     CDS             complement(<1..408)
                     /locus_tag="IXH60_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991658.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTK"
     gene            complement(463..1116)
                     /locus_tag="IXH60_002781"
     CDS             complement(463..1116)
                     /locus_tag="IXH60_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1991659.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            complement(1106..>2320)
                     /locus_tag="IXH60_002782"
     CDS             complement(1106..>2320)
                     /locus_tag="IXH60_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1991660.1"
                     /translation="HPERKVKTIHIAGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIE
                     TFNERIAINGKSIPDNDLIQLVKKYQPLIKQLDQFEAVAGITEFETLTAMAFDYFLMK
                     KVDVAIIEVGLGGLLDSTNVAVPMLTGITTIGLDHTEILGDTIEEIAAQKAGIIKQGV
                     PVVTGNIAPEAFTVIQQTAKAKNAPLYVYGENYKINYLHPDEMWGEVFEFFNEIGKIP
                     QLTTPLLGQHQAENAGVAIELYFVYCQLEKLPFREKDVLKGLKKSRWPGRMERLSNEP
                     LVVLDGAHNTHAMNRLVENLKKEFKGYQINILFSALRTKNVREMLERLQQVPNVHIYL
                     TTFEHPKAIELSEVSDLASERVSIVSLWQFGLADILEKMTNEDVLVVTGSLYFISEVR
                     HLLIQLGGDHEV"
BASE COUNT          649 a          502 c          396 g          773 t
ORIGIN      
        1 cttcgtgtcc aaataaatac aaaccaaatg ttcttgtttg tgatctttca tttccagaat
       61 caattgctgg gccaattcgt agctggtacg aaccggtgga gccgctcttt catgatccgt
      121 ctgaattcgt cgacctaatt cgatcaaggc tttgatttcg attgctttca cgcgtcctat
      181 ccctcgaatc tcttctaact catgcaaagt agcctgtcgt aaagaggcca gtcctccaaa
      241 cgtttttaac agatttccag caatcgacat cacactataa gggtgttgac cggtacgcag
      301 taagattgcc aaaagctcct gatccgataa agcttccgcg ccataaattt ccatacgttc
      361 ccttggtaaa gagctagtag gtacttcttt gatcaatcgt tttcccaaaa aaacatctcc
      421 tttctatctt atctaaaata agatacgaaa agagatggat ttttatttta acaatgcagg
      481 gatttcatgt aaagaagaaa caaccccagc tgtttttttg atcaattctt gggatggtgg
      541 aagcagatct ggtaccatga ctaccgggat ctcagccgca gaagctgcta aaataccatt
      601 ttgtgaatct tctaaaatca atgtttcttt cttcgctgta cctagcttct gattagctaa
      661 caagaaaatt tctgggtctg gttttgctcg ttgaacattt tctgcagaaa caatcgtctc
      721 aaatcgttca aacagattgt tcttcttcaa taaaagttca atgactgctc gttggttact
      781 ggaagcgaca actttgggaa tctgatgttc ttctaaaaaa gagaggagtt ccaacacacc
      841 gggcttcatt tgtaccgctc cttgcgaaaa acgttcaagc gtttcctggt atgaatcatc
      901 tatgaatctt tgtacacttg ttttgccaaa ttccgagaaa atcatatgat aattcgccca
      961 tacttcttca tctgaaacac ctaagaattt taaatacagg tctttatcat aaggaaaacc
     1021 catcttgtct gctactactt gtgtggcttc gtaatacact aactctgtgt cgaacagcaa
     1081 accatccatg tcaaaaatca ctgcattata cttcatgatc tcctcctaat tgtattaata
     1141 aatggcgaac ttctgaaatg aaataaagtg aaccggtcac aactaatacg tcttcatttg
     1201 tcatcttttc caatatatct gctaatccga attgccacaa tgaaacgatt gatactctct
     1261 cacttgctaa atctgatact tcacttaatt cgatggcttt tggatgttca aaggtcgtca
     1321 aataaatatg gacatttggt acttgctgca atcgttctag catttctcta acatttttag
     1381 ttcgtaaggc ggaaaacaaa atgttgattt gatatccttt gaattctttt ttcaaattct
     1441 caacaagccg gttcattgca tgcgtattat gggcaccatc taaaacaact aatggctcgt
     1501 tactcaaacg ctccattctt cctggccatc gagatttctt caatcccttc aaaacatctt
     1561 tttctcggaa aggcagcttt tccaattgac agtaaacaaa atacaattcg atggctactc
     1621 ctgcattttc agcttgatgt tgccctaaaa gcggagtggt taattgaggg atctttccta
     1681 tttcattaaa aaattcaaat acttctcccc acatctcatc aggatgcaga tagtttattt
     1741 tataattttc cccgtataca taaagcggtg cattttttgc tttagctgtt tgttggatga
     1801 cagtaaaagc ttctggggca atatttcctg ttactactgg aacgccttgt ttgatgattc
     1861 cagctttttg tgcagcgatt tcttcaattg tatctccaag tatttcggta tgatcaagtc
     1921 caatcgttgt tattcccgtc agcattggta cagctacatt ggtagaatcc aataagccgc
     1981 ctaagccaac ttcgataatc gctacgtcca ctttcttcat caaaaaataa tcaaatgcca
     2041 ttgctgttaa tgtctcgaat tcagtaatac cagctactgc ttcaaattga tcaagttgtt
     2101 tgatcagtgg ctgatatttt tttacaagct ggatcaaatc attgtctgga atgcttttgc
     2161 cattgattgc gatccgttca ttgaacgttt cgatataagg tgaagtaaat gtacctactg
     2221 tcagcccagc ctcttctaat aggcttctca aataagctac agtagaacct tttccgtttg
     2281 ttccagcgat atggatcgtt tttacttttc tctctggatg
//
LOCUS       DACEZN010000247         2286 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEZN010000247 DACEZN010000000
VERSION     DACEZN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.251"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            74..256
                     /locus_tag="IXH60_002783"
     CDS             74..256
                     /locus_tag="IXH60_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991661.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            240..425
                     /locus_tag="IXH60_002784"
     CDS             240..425
                     /locus_tag="IXH60_002784"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1991662.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            668..2188
                     /locus_tag="IXH60_002785"
     CDS             668..2188
                     /locus_tag="IXH60_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1991663.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          785 a          391 c          469 g          641 t
ORIGIN      
        1 ataggatagt atctcgtacg ccgattatct ttcttctata attaaactat caaatataga
       61 aggagaaaaa cgaatgaata aaaaagatat tgtaccagtt caattttcaa gtgcggctca
      121 aaaatcgcaa attgacacca agccaaaact agcttgcaga atcaaatcgg aagataagga
      181 aatatttatt tataatggaa tggataaata tcttttgtat accttgttaa aagagttgaa
      241 tgccaatgct cattgatgca actgcattga aacatatttt tatcgtttgt ggaaaaacag
      301 atcttcgaaa aggaattgat ggactagcag ctttcgttat agaagaatat gaccttgatg
      361 tttacgatca agcgcttttc ttattttgcg gtacgaagaa agatcgcttc aaggctcttt
      421 actaggatca aaatggattt gtattaatgt acaaaagatt tgaaagtggg taccttcaat
      481 ggccgcgttc cagaaaagaa gtcttactga ctggcctttc aatcgaagaa aaatgtacgc
      541 taaaagaaac gaaaaaaggt gcgatatatt agacgaaaac cttgttaaat caaggatttt
      601 gtctgttttt cgttgtcata aaatggtata atagatgtaa gaaaatcgct agaatggtgg
      661 tgtaaaaatg acaaaaatgg aatctgaact tcttgtacaa atcaagcaat taacacagtt
      721 gcttgaaatc cagcaacagg aaaatactct tcttagagaa caaattgctg aaatgaatcg
      781 acgcttgttt ggacgaaaaa aagaaacacc acccgtagat gggcaaatcg atttattaga
      841 cgactcaact tttaatgaac cagagcacac tggacaagaa agccaggaac ccattacggt
      901 atcttctttt aaacgcagaa aacgcaaggg actcaaagca ctttcattag aaggtctgcc
      961 tgaagttcct attcattatg aattaaaagg tgccgattgt ttgtgtgaag actgtggtca
     1021 tactttacat gatatcggtg cgacaaggct tagagaagag ccattgtatg ttcctgccca
     1081 tatagaaaaa caagtcatct accaacatgc ctatcattgc agagaatgtg aagcattagg
     1141 agaaacaaaa atcaaaaaag cgcctgttcc taaaccgttg atcaataata gtctaggttc
     1201 ctcttcaatt gtgacacaga gtatttggga aaaatttgtt aaaaaggtgc cggcatatcg
     1261 acaagaaaaa gaatggcatg cacttggttt ccctcttgat cgtaaaaaaa tcactaattg
     1321 gcatatcaag gtaagtgaaa tggttttaga gccaatctac aattgccttc atcaagaatt
     1381 aagcaatcag gaggtcttac acgcggatga gacaagctat cgggtattga atactcataa
     1441 agaaaagacc ttttactggt tatatgcttc aggtaaaagc gaagaaaaac aagttgtgct
     1501 ctacgagcac gcaaattcgc gttccgttga tgagccaaaa agttttttag aatcatttag
     1561 cggtttcttg catactgatg gttacgcggc ctatggaaag ctgccggtaa acaatgttta
     1621 ttgttgggcg catgttcgca gaaagttttt tgaagcattg ggtaaacaac cgtccccaaa
     1681 aagtcatgcc aaaataggat tagacttctg tgacaagatg tttcgaatcg aacggacctt
     1741 taataagctt agcgcagaag aaaggtttgt agcgaggaag gaaacgttgc agcctgtgat
     1801 gactgaattt tttcattggt gtgagagttt tgcttatttg ccaaaatcgc agcttggaaa
     1861 ggcggttcaa tacgcagtca atcataaaga cggacttcag attgtgttgc ttgatggacg
     1921 cttggaactt tcaaataacc gagcagagcg tgccatcaaa gagttagtga tcggtcgaaa
     1981 aaactggcta ttttctaaaa gcttaaaagg cgctagatca aatggaatta tattgagtat
     2041 tatccagaca gctgttgcga atgggttaaa catccgcaaa tatttaaatc atctttttac
     2101 agagattccc aatctgtctt cgatgacacc tgaagcgcta cgcgcttatt tgccatggaa
     2161 tcagcagata caagaaatct gtaaataata taagccacaa ataacctgat ttgagtgtat
     2221 caaatcgggc tatttgtgtt taggtaccat tctatgacac gcttacgctc aaaactactg
     2281 aattat
//
LOCUS       DACEZN010000248         2283 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEZN010000248 DACEZN010000000
VERSION     DACEZN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.252"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            12..>2283
                     /gene="esp"
                     /locus_tag="IXH60_002786"
     CDS             12..>2283
                     /gene="esp"
                     /locus_tag="IXH60_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1991664.1"
                     /translation="MISKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVKAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTDNWEIRLQLDERLAKYVENIQVDSKKVAGISYRRTF
                     VRINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKNLDEIFQEIGED
                     NLKSDRLMYRIYLVSHQDNDKIVPGIESTGYFITDQDNFYNKLDVSENNPDQFKHGSV
                     STKYEKPNVQTKDGSGSTGANGAIILDHKLTKEKNFSYSVSATGTPWYANYKIDERLV
                     PYVAGIQMHMVQADRVVYDVSFESGTKVADLAIERREGHENYGVGSITNNDLTKLIDF
                     SNASPRPVVIRYVLQLTEPLDEILAEMKTAAQVEGNEPFGEDFIFDSWLSDTNKKLIQ
                     NTYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAG
                     TGHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSNKLIAKDELKVQIVS
                     PGFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTS
                     NVADSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTIQSGKPTFD
                     KPDVPLANDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGETEPITGTIPIKIVYQ
                     DGSVGTTDLAVTVSKDI"
BASE COUNT          813 a          350 c          475 g          645 t
ORIGIN      
        1 tgatttgaat aatgattagc aagaataata agagagtatt tctagaaaaa acaaagaaac
       61 gagtattgaa gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt ttggttggtg
      121 ttggtcttgt cttgggaaca actgaactag ttaaagcaca agatgaaatt tctccaagca
      181 ccccacttga aacagcgata agctccgtac aagtaggtga caaagtagct tcggggaata
      241 cttttcagga gaatcctggt tatacgaaaa attataactt ttcggattta caatttagtc
      301 ctcaagaact aactggagac acactaaaag gcaatacaat tggatttgaa gtttacggaa
      361 aacataatat tgctgcttct accgataatt gggaaatccg cctccaattg gatgagcgat
      421 tagccaagta cgttgaaaac attcaagtag attctaaaaa ggttgcgggg attagttaca
      481 gaagaacttt tgtccgaatc aatgattcat taggtagacc cacgaacatt tggaaagtta
      541 attatattcg tgcaagcgat ggattatttg ctggggcaga aacaactgat acacaaactg
      601 ctcctaacgg ggtaattaca tttgaaaaga atctagatga gatttttcaa gaaattggtg
      661 aagataacct taagagtgat cgtttgatgt atcgaatcta tctggtaagt catcaagaca
      721 atgataaaat tgtacctggt atagaaagta ctggttactt tataacagat caagataatt
      781 tctataataa gttagacgta tctgaaaata atcctgatca atttaaacac ggatcggtaa
      841 gtaccaaata cgagaaacca aatgttcaaa cgaaagatgg atcggggtct actggtgcta
      901 atggcgctat aatcttggat cataagttaa caaaagagaa aaatttctct tactcggttt
      961 ctgcaacggg aacgccttgg tatgctaact acaaaattga tgaacgttta gttccgtatg
     1021 tagcgggtat acaaatgcat atggttcagg ctgatcgagt agtatatgac gtttcatttg
     1081 aatcaggaac aaaagtagct gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg
     1141 gtgtaggttc aataactaat aatgatttga ctaaacttat cgatttttct aatgcaagtc
     1201 cacgtccagt tgttattcgt tatgttcttc aattgactga accattagat gaaattttag
     1261 cagaaatgaa aacagctgca caagttgaag gaaatgaacc gtttggtgaa gacttcatct
     1321 ttgattcttg gttgtcggat acgaataaaa aattaatcca gaacacttat ggaacaggtt
     1381 attattattt acaagatatt gatggtgatg gaaaccctga cgataaagaa gagagtggag
     1441 acacgaatcc atatatcggg aaacctgaat tagaagaagt atatgatgtt gatacaacag
     1501 ttaaggggaa agtattcatc cacgagttag cgggaacagg tcacaaagct caacttgttg
     1561 ataaagaagg tactgtatta gcagaaaaaa ctattgctcc aaatgaaaaa gatggagctc
     1621 caatttcgga tactgtagaa tttgaattta cgggtgtgga ttcaaataaa ttaattgcga
     1681 aagatgaatt aaaagttcaa atcgtttctc caggttttga taaaccagaa gaaggttcaa
     1741 ccgttatcaa ggaatcacca aaagcggttg ataaacaaac cgtggtagtt ggatttaaac
     1801 cagatgctaa agaatcaatt cggaataata aaaacttacc tgaagatgca gagtattcat
     1861 ggaaaacaga gcctgatact tctaacgttg ctgatagtac gaaaggtatt gtaactgtta
     1921 aaatcggaaa tcgaactttt gacgtagatg tagagtttgc tgtaaaagct tctcaagcta
     1981 tggaaaatga tgcaacatac gtacctataa caacaacccc agaaacgaca attcaaagtg
     2041 gtaaacctac atttgataaa ccagatgttc ctctagctaa cgatgccttt tcaattttag
     2101 atgtttataa taaggacttc ggcaatgcaa gtgttgacgc aaatactggt attgttacat
     2161 tcactccagc caaaggggta ggagaaacgg aacctattac tggaacaatt cctatcaaaa
     2221 ttgtttacca agatggttct gtaggtacga ccgatttggc agtaactgta agtaaagata
     2281 ttt
//
LOCUS       DACEZN010000249         2208 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEZN010000249 DACEZN010000000
VERSION     DACEZN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.256"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(307..972)
                     /locus_tag="IXH60_002787"
     CDS             complement(307..972)
                     /locus_tag="IXH60_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1991665.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1098..>2208
                     /locus_tag="IXH60_002788"
     CDS             1098..>2208
                     /locus_tag="IXH60_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1991666.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIY"
BASE COUNT          660 a          361 c          396 g          791 t
ORIGIN      
        1 aaattgatct aaattatgtg cagcttgact acgtaccaat ctatattact tactagattg
       61 gtcgttaatc tattcggatc gaatgagcaa aatatttttg tgaatgtcca tccttgatta
      121 tgattccaaa aagaatcatt gggaggctgt ggcgtaaata ttatactata agccagaaat
      181 acctatcatt ctgaactttt ttatttagga actttcatct caaggctttt tcaattaatt
      241 tgtacccctg ttttttggat aagatcatgt gcattgattt tttctatctc tacgttcatc
      301 tcctacttaa tgcctatctg gctcctgagg gcttcttttt aaggcgtcgt catagtcttg
      361 gctgaggatt tcttggtaat ctgagggaat ggcttcccaa gtgagcaggc gaacgttcat
      421 gacgtgtttt cctttgtgtt tgattacagc atggtttttc tctttttcta cctctccagc
      481 gtgaaaagac agccatgtac catggccaaa gatatcatta catacccaac gttcgatgga
      541 attgtccacg tatcctacag ggagtgtggt atagccgatt tgtggtttca aaaaaggatt
      601 gaatcgatcg atcgtttcat ttttgtattg ataattgata agaaaaagat agaccaaaca
      661 aaagacgagc agaaagaaag atagtgtctt tttgagtgag aagggttgtt ttgatgaata
      721 agatttcttg ttaacaagag gttcatctgg caatgttggt tctattgatt cggtagctga
      781 ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga tgtaaaagtg acagcatttc
      841 aaggtctggc attgtacgag aattttccca actagaaatc gttttatcgg aaacaccaat
      901 tttttcgcct aattcttttt gcgtaagctt acaatttctg cggtaagttc gtattttttc
      961 tccggtgttc atcttcttct cctttcgatt ctacagtata tagatttatt ctacatgacc
     1021 tatacacttg atagatacgt ttttttgatg aaaaacggta tgattttatc aaagaatcat
     1081 ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac agttttactc acaatcatag
     1141 tgaccggtat catgagtggt tttgccttgg ttagtataaa taaacaggta ctctatcgga
     1201 caaatgaatt tttccatcaa acagatactt atactccgat tcgattgcca caacatttta
     1261 ttgggcaaga agaatatgag cagctaatca tggtatttga aaatgtctca tcagaaactg
     1321 agctttctta tatgaaaaaa acagtaaatg aatccaatga agtagataca tcttggaact
     1381 tcaagataca gccaaagctt gaaatgacat tgtattatca aggggatgtc aaatcttctc
     1441 acgagaaggt accctattct aatacctcct ttcgcgtaac gtccattgaa gaatcgatca
     1501 aaataaaaga ttttgaaggt gaatttttct tgatgacgag agaaaaagaa gcctatgaac
     1561 ggttcatttc actattcact gatcgattca atcaagcatt tcacactgat tatgccacta
     1621 atatttttga agactttgaa aatagctatt cgtattcggt tatttcaaat acagacgtat
     1681 ataattatcg agtgtattta actattggat tgattctttt attccttttg ttatcatcct
     1741 ttttcttatt gatgaataaa gaaatcagtt tattgttgaa taatgacttt tcattacatc
     1801 aagtattgtg gtgtttattg gggaaaaaga aaatcattat tacattcata attgcaggaa
     1861 tcattggtgg ttttattttt atgtattata aggagcaatt tctttatttg ttccagatgt
     1921 acgttttctt gttcagcgga atatatggta tctctttgat ttctctttta ttagttgaaa
     1981 aaatcgcttc gttaagagaa gcgttcaact atatccaata cattcttttt acatttggat
     2041 tgttgttttc tcttcttttg accgttgcca atgtcgaatt agcaactatc gttttttctt
     2101 cttcacagtt agctgtttcc catgaaccac caaaagaaat cgctggtgag gactatcatg
     2161 tattttatcc agtaaccatc gggaaaaacc acgtggattt tatttatt
//
LOCUS       DACEZN010000250         2194 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.258, whole genome shotgun sequence.
ACCESSION   DACEZN010000250 DACEZN010000000
VERSION     DACEZN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.258"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            278..802
                     /gene="infC"
                     /locus_tag="IXH60_002789"
     CDS             278..802
                     /gene="infC"
                     /locus_tag="IXH60_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1991667.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            874..1074
                     /gene="rpmI"
                     /locus_tag="IXH60_002790"
     CDS             874..1074
                     /gene="rpmI"
                     /locus_tag="IXH60_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1991668.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1127..1486
                     /gene="rplT"
                     /locus_tag="IXH60_002791"
                     /pseudo
     CDS             1127..1486
                     /gene="rplT"
                     /locus_tag="IXH60_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
     gene            1838..>2194
                     /locus_tag="IXH60_002792"
     CDS             1838..>2194
                     /locus_tag="IXH60_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1991669.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
BASE COUNT          735 a          384 c          478 g          597 t
ORIGIN      
        1 aatcactgct agaaagcctg ctaatctaat cattttcaga aaaggaagac aaactgtatg
       61 aaaaacattg acaaaaagat tagtttttgc tatactataa tggttgagaa ataaaagcag
      121 aagcacccgc ttctcgcctt agttgataat gtcactgggc tagatagttt tcactggttc
      181 tattaggtga agtacgcgcg ggttctgtaa gagaactcgt ttttttattg ccgtttattc
      241 ggtaagcttt tctttctctc ataatatttg gaggtgaatg accatagcaa aggatatgat
      301 ggttaacgac ggcattcgtg cacgagagtt acgattgatc ggttcagatg gtgagcaatt
      361 aggagttaag acaaaagcag aagcattgaa tattgcagaa caagcaaact tggatcttgt
      421 gttagttgcc cctggtgcga aaccaccagt tgcgcgaatc atggactacg gtaaataccg
      481 tttcgaacaa caaaagaaag accgcgaagc gcgtaaaaaa caaaaagtga tcaatgtaaa
      541 agaagttcgc ttaagtccta cgatcgactt gaatgacttc aatacaaaac ttcgtaatgc
      601 acgtaagttc ttggaaaagg gcgataaagt gaaagcttct atccgtttca aaggccgtgc
      661 cattacccat aaagaaattg gtcaaaaagt cttgaatcgc ttagctgaag aaactgcgga
      721 tattgcaaca gtggaacaaa aagcgaaaat ggacggacgc agcatgttct tgacgctggc
      781 accgaaaaac gacagtaagt aatttgaatg actaactggc tgttggacag ccgatagtcg
      841 gataacgaaa gattttgagg aggaaatata gtcatgccaa aacaaaaaac acaccgcgga
      901 tcagcaaaac gtttcaaacg tactggtaaa ggcggattga aacgcttccg cgcgtttaca
      961 agtcaccgtt tccacggcaa aacaaaaaaa caacgccgtc aattgcgtaa agcaagcatg
     1021 gtttctagtg gcgacttcaa acgtattcgc caacaactag caaaaatgaa ataagaagta
     1081 caaataacag aatctgaaga tttattttta ttaggaggaa ttatacatgg cacgtgttaa
     1141 aggttgaaca gtaactcgta aacgtcgtaa aagagtgcta aaattagcaa aaggttacta
     1201 cggttcaaaa cacactttat ttaaaacagc aaaagaacaa gtaatgaatt catacaacta
     1261 cgcatatcgc gatcgtcgtc aaaagaaacg cgacttccgt aaattgtgga ttgctcgtat
     1321 caacgcagca gctcgtatga atggcttgag ctactcaaaa ttaatgcacg gtttgaaagt
     1381 tgctgaaatc gacatcaacc gtaaaatgtt agctgacttg gctgttaacg atgcagcagc
     1441 atttactgca ttggctgacc aagcaaaaga agctttggct aaataagcta aacaattata
     1501 aagaggaaag aagataacga aaagttatgt tctttcctct ttttttattt acaatcctcc
     1561 atcaaatgaa acaggacaat aacgtataag tctaaaataa cataattaag gttttttaat
     1621 tgaaaactga acatttatgg tcatccttta atgaaagcgc taactacata agttagaatg
     1681 agtttgactg atatattagt ttgcttatca gaaaaacaaa atggacggag gaataacatg
     1741 agtgatatta ttattggtat tttgttagtt atctcattct ttttcatggt ctggtactgt
     1801 gtaaaaggct acaacttgat ggtcggcttt tgtcatcatg gctactgtat ggatgggatt
     1861 agcgctagtc ggaaacacgt tttcaccaaa ctcggcgatg gaagggcaag gagtaattga
     1921 tgtgttgacg catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa
     1981 tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct
     2041 gattcgaaag gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg
     2101 tattgtaacc gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc
     2161 agttattgtg ttgccgatct tgatgtcatt aggt
//
LOCUS       DACEZN010000251         2189 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.259, whole genome shotgun sequence.
ACCESSION   DACEZN010000251 DACEZN010000000
VERSION     DACEZN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.259"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(325..528)
                     /locus_tag="IXH60_002793"
     CDS             complement(325..528)
                     /locus_tag="IXH60_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991670.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="IXH60_002794"
     CDS             complement(987..1952)
                     /locus_tag="IXH60_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1991671.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DACEZN010000252         2169 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.261, whole genome shotgun sequence.
ACCESSION   DACEZN010000252 DACEZN010000000
VERSION     DACEZN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.261"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(310..807)
                     /locus_tag="IXH60_002795"
     CDS             complement(310..807)
                     /locus_tag="IXH60_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1991672.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IXH60_002796"
     CDS             complement(847..1656)
                     /locus_tag="IXH60_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1991673.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..>2169
                     /locus_tag="IXH60_002797"
     CDS             1990..>2169
                     /locus_tag="IXH60_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1991674.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETV"
BASE COUNT          661 a          376 c          388 g          744 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggta
//
LOCUS       DACEZN010000253         2099 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.262, whole genome shotgun sequence.
ACCESSION   DACEZN010000253 DACEZN010000000
VERSION     DACEZN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.262"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            169..1539
                     /locus_tag="IXH60_002798"
     CDS             169..1539
                     /locus_tag="IXH60_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAQ1991675.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IXH60_002799"
     CDS             1560..1844
                     /locus_tag="IXH60_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991676.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          728 a          307 c          444 g          620 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttc
//
LOCUS       DACEZN010000254         2096 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.263, whole genome shotgun sequence.
ACCESSION   DACEZN010000254 DACEZN010000000
VERSION     DACEZN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.263"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(106..534)
                     /locus_tag="IXH60_002800"
     CDS             complement(106..534)
                     /locus_tag="IXH60_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991677.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(562..1200)
                     /locus_tag="IXH60_002801"
     CDS             complement(562..1200)
                     /locus_tag="IXH60_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1991678.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1204..1974)
                     /locus_tag="IXH60_002802"
     CDS             complement(1204..1974)
                     /locus_tag="IXH60_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1991679.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT          657 a          399 c          297 g          743 t
ORIGIN      
        1 atatattttt atacaactaa aaatgaatat attaaaacta ccaaaaataa cttttaacgc
       61 tgtttcatta tatgtttttt ctattttata taaacttttg gatacttaat accaaccggt
      121 tggattgtac caacatgaga atgtgtcagt tctatttcct gtcgcacttg cataggccca
      181 tactcctttt gttgcatttg ctgatcgaat agagtttcca actttcgctt ttgcaccgtg
      241 acgattatag acacgactat agtggttaga atgacctgtt tttctcgtac aagttgtatc
      301 actataataa acccatcttc cagagctatg attaattgct gttctagaaa tttcccggtc
      361 tactggtaaa tttgacttaa taatattacc tttagcatca aaatgaatct ctcctggctt
      421 taaagatttg taatagattt caccgtgaac tgaatttgct tccacttcag atgacatagc
      481 gtttcctcca acaaaaccta tccctagtgc acaaataata actgcttttt tcataaaacg
      541 acatcctttc ctttttgatt tttaaagcat gatcttcttg tttcctattt tggtcagata
      601 tggatcatgc gtgactacga taactaacgt accttgggaa gcttgttgcg ataagatttc
      661 aactacttta tccttatttc ttttatccaa tgacccagtc ggttcatcgg ctaaaatgat
      721 tttgctattc ttcaataaga ctctcgcaca ggcgattctt tgcttctcac caccagataa
      781 tgtgtagata ggcgtactta gcttcttgtt tatatttact tttttcaatg cctcatactt
      841 tgctgttcgc ttatctgaaa catttaaatt cgaaaacttc aatccaatat ctaaattaaa
      901 atcgattgtt tcatattcaa tcaacccaaa atcttgaaaa agaaaagata tggtatttct
      961 ttttatcaag cgcttctctc tcccagttaa ttgtttcgtc tcttgatcga acaaatagaa
     1021 ttgtcctgaa ctgaactgat cattcaatcc taatatattc aataacgtac ttttacctgc
     1081 tccactttct ccaacaacag tcacaagatc cccagcatcc gcagtgaaac gatccaaatg
     1141 aagaatcgtt ttgtttcgaa tgattttttt cgcacctttt atcactatca acggctccaa
     1201 ttgttaacct ccattcatca gcaataactg acttttcttt tctgtagaca aaataaaaac
     1261 aactgaaaga ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc
     1321 tctttctata agaaaaagga agattacact tgtcgttccc catttgatga ggttcatcat
     1381 gaagaaatct ttatacgttt caaagagcga ataaccactt gaacgaaaga tagcgatttt
     1441 ccgacaattg tagacaaaat atatgcataa catgacatag ctcaacaaga agaaagaaaa
     1501 gaaagtgagt aaaaatatct gtatcaagta atgaaatgat ctgcttaaca ctttgacagc
     1561 tatctcttca gtctgagtat acggaaatga taactgcaag ttatcacgta actgactttc
     1621 ttcaatcagc tcaaataacc tttctttcga caaagagcct atcttgatct ttaacggtgg
     1681 atagatttct ccgctgagaa tatttctttc caaatacata ctgtttttca atgtcagtat
     1741 ttcaacaacc ggataatgtt cgatccaggg gatagaagga ataaatgtat aaatgggttg
     1801 attgtttttt ataaatagaa atttcggttc tttttcactg atttcttgat aatattctgt
     1861 tatttgcgcc aggtcatttt ttaaatctgt atgtctatat cgttcaggga tcaatataac
     1921 agggtgcgtt tctttttcgg ttatttggat caaccttcca gagacatcaa tcaaaggaaa
     1981 tttcttaaga taattcagat ttatttttat ccctctgcca aactgttcat tttcttcatt
     2041 caagtaatct gatacattga ctaatatact tccgttttta ttcaaagttt catata
//
LOCUS       DACEZN010000255         2094 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.265, whole genome shotgun sequence.
ACCESSION   DACEZN010000255 DACEZN010000000
VERSION     DACEZN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.265"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            185..703
                     /locus_tag="IXH60_002803"
     CDS             185..703
                     /locus_tag="IXH60_002803"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1991680.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            831..1514
                     /locus_tag="IXH60_002804"
     CDS             831..1514
                     /locus_tag="IXH60_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1991681.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1823..1896
                     /locus_tag="IXH60_002805"
     tRNA            1823..1896
                     /locus_tag="IXH60_002805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1857..1859,aa:Arg,seq:tct)
BASE COUNT          727 a          315 c          416 g          636 t
ORIGIN      
        1 tgttagggta aacgtttttt aaactaacgt caattattta taataaaaaa tgagataaag
       61 atgaggaaat agaattagaa acatataatt attaaaggga aacattttta cagctataat
      121 ttgaaaaaaa tttatcgtta ttatatgatt aagaaggatt gaataaagaa agaagttgaa
      181 aacgatacct gattacaaaa aaataacttt tataagttct ataacgttga ttatattttt
      241 aattcttatt cttctcgtcg tcttggttcc ccgaattttt ggtttgactc cttatattat
      301 tgaggacaaa gaaatgagtc catattatgc acaaggaaat ctgatatatg tacgaccgaa
      361 aaatacaaat gagattttag tcggagatat tattacatat tatgaaaatt caggaaaaaa
      421 aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac gaagattttt atacaaaagc
      481 tgatacaaag acttatattg agcctggagt agtaaaaaag agaaatatta tcggtagtcc
      541 catctttcag attccatata ttggactagt cctttcaaaa acggcatttg catggattca
      601 ggggttattc tttacgattg ctttttgcct aacaggaatc acagcatgga atacctttgt
      661 agagttgaaa aagaagcgga gacgaacaca gaaatttttg taagaatgtt aaaaacataa
      721 atgtattcca gagatccaac gactcttctg aacatgaaac acgagaaaac actcatgcag
      781 cttgtagatt tagtcagttg ttcgtataat gtgtgaagag gaggagtagt atgcgcaatg
      841 aaatgatgca tcgtcctgta gtcaaagaag aactattgga ttttatgcgt acccagcaaa
      901 aaaaattacc gggtgagcta ggcaaactgg aagaagaagc tcatgtagca gaagtaccag
      961 ttattcccca tgaaacagtt gtatttttga aatttttact tggacagctt cagcctgaac
     1021 gaatcttgga gatcggagca gctatcggtt tttcatcaag tgtcatggcc acgacaatcg
     1081 gagaaaacgc tcatgttacg acaattgatc gtttcgatgt catgatcgag aaagctaaaa
     1141 aaaactatga acgtctagga ctgacagata aagtgacttt gcttgaaggt caagcagcag
     1201 atatcttgcc ggagttgtct ggtccgtatg actttatttt tatggatagt gcaaaatcaa
     1261 aatatattga atttttacct gaatgcttac gtttgttaag aaaaggtggc gttttgatgg
     1321 tagatgacat cttccaagga ggaacgatct tgctcccaga tgaagagatt ccccgtggca
     1381 aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt tgtgatggat catcccgatt
     1441 tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt aatgacaaag gaatcagaaa
     1501 caatcgagtt gtgatacatg attatggcag cctgtagata atcgattgaa tcgagggctc
     1561 aattcgaact tgatgtaaaa gtttctatat ttgtaagtca attaaaggat acaggaaatc
     1621 taaataaaca ggagtagcat gaagtcaatg tagctcctgt tttcaattga taaccttaga
     1681 acagctgaag gagaatgtct cgttttaaaa gattgattta gtagttgggc caaaataatt
     1741 cattatttgc taattaaata actatttcgt attgacagaa gcaatttaat tatctataat
     1801 tattgatgtt ccgctgattc ttgtcatagt agctcagctg gatagagcat ccgccttcta
     1861 agcggacggt cgggggttcg aatccctcct gtgacgctaa aaaaccgtta gaaactgagg
     1921 tttctaacgg tttttctttg ttttttgtgc caatattgtt tgttttcaaa ggcaaataaa
     1981 ataaaaaaac atccttatcg agtacaatta taaggagcaa aaaaataaaa ctcatcagaa
     2041 aagaggaaga agatatgacg atactatcta gaattttagt gactctagtt gcaa
//
LOCUS       DACEZN010000256         2050 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.266, whole genome shotgun sequence.
ACCESSION   DACEZN010000256 DACEZN010000000
VERSION     DACEZN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.266"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..177
                     /locus_tag="IXH60_002806"
     CDS             <1..177
                     /locus_tag="IXH60_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991682.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IXH60_002807"
     CDS             190..690
                     /locus_tag="IXH60_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1991683.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>2050
                     /locus_tag="IXH60_002808"
     CDS             787..>2050
                     /locus_tag="IXH60_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ1991684.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQK"
BASE COUNT          660 a          280 c          463 g          647 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac
//
LOCUS       DACEZN010000257         2047 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.267, whole genome shotgun sequence.
ACCESSION   DACEZN010000257 DACEZN010000000
VERSION     DACEZN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.267"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(128..226)
                     /locus_tag="IXH60_002809"
                     /pseudo
     CDS             complement(128..226)
                     /locus_tag="IXH60_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(491..946)
                     /gene="tnpA"
                     /locus_tag="IXH60_002810"
     CDS             complement(491..946)
                     /gene="tnpA"
                     /locus_tag="IXH60_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1991685.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1189..2047
                     /locus_tag="IXH60_002811"
                     /pseudo
     CDS             1189..2047
                     /locus_tag="IXH60_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          646 a          387 c          371 g          643 t
ORIGIN      
        1 agtttatgat ccttttgatt tttatattaa tttatctgta tttaatacag attttattta
       61 ttagttcatc tgcctattgt attttatttt gttgccttac catctgaagt aaatatactc
      121 ttaattgtca aattaagtta gacagatcat caatacttac gtaactcaaa aatacttcca
      181 aaggtgttcg ataatccagt gattttctag gaagattatt tctgcgccag cattttctaa
      241 tgtttgaatt tgtgttttgt aatcttggtt gcttgtgcta attcttgaat agccgtaaat
      301 tgccaaaatt tcatcaccct ttctggacat aagtttctga caccagttaa aatcaagtct
      361 atcagtaacc catacacccg tgaggacggg tgtattttta atccccctag aaggggtcga
      421 cactcgctgc cccttaaaag gggattttta aaagaccgcc aactgctcat cttcactcac
      481 ttacttctct ttatttttta aatggttcta cgtactctct tttacttata atatatcgaa
      541 tgcgatcatc agcttcttgt tcacggatgt atttttcatt tgtcttttga ttcaatccga
      601 ctgtactcac atagtatctc ttcgaccaaa aggatttgtt tccgtattta tatttgagat
      661 ttgcgtgctg ctcgtggatt agtaccgcac tacgaccttt cagatacccc ataaaactgg
      721 atatacatat ttttggtggt actcgaacaa gcatgtgaat atgatctagc attgcatgtg
      781 cttctatgat ttccacatct ttcatttcgc acaacttcct aagtattttt ccaagatctt
      841 ttcgtaattg cccatagatt acttttcgtt gatattttgg tgtaaaaata atatgatact
      901 tacaattcca acgagtatat gctaaacttt tatcgtcatt tgacatgaaa aaactccttt
      961 cgatctgatg agcggttggt agccactcac tagtatatcg aaaggagttt ttatcgctaa
     1021 agatacttct tccacgcgta gaaacgcgcg gttttcttgt ttcgcacgct ttacgcacaa
     1081 aactgactga agtcattaag aaaataaaag ggtctaaaac ttttaagttt tgtaccgacc
     1141 cccaaaagtt agaccaaaaa atctaacttt tggaggtcgg tattttttat ggctaagtat
     1201 acttttgaac ttaaattaaa gatcgttcat gattacttgg atggtaaagg cggaagtgat
     1261 tatctggcaa agaaatattc cattaaggct ccatcacaag tgaagcgctg gatcaatgcc
     1321 tatcaagaat ttggtgaaga gggtctagta agaaagcgac aaaagaaaaa atattctgtt
     1381 caatttaagc tggatgcgat agagttatat ctaacaagtg agctgtccta tcgtgaagtt
     1441 gccaacacac ttaacatgaa caaccctaat ttgatcgcta gttggatgcg tcaattccgt
     1501 gagggtggga tcgatggact ctcaaaaacg aaaggatgtc cacctatctt gtctaagaaa
     1561 aatgaaccca aaaataagaa aaaatctaca actaaagcaa cgtctaaaga acgtgaacgc
     1621 atcgaggagt tggagaaacg agtccgttcc cttcaaattg aaaatgcctt tttaaaagaa
     1681 ttgaggaagc tgcgaaaaca ggaagcccaa caacgacgaa tgaagcaatc gcacgaatca
     1741 tcgcaagcct ccgaggaccg tttaaattag ttgagcttct agaaacttta aagttcccta
     1801 aggcaatctt tatgtactgg caaaaacgat tggaccgtaa aaatccagat caagagattg
     1861 aagcggaaat gcttaaagtt cgggagaagc acaaggatta tggctgttta cggatgacca
     1921 atgaactaag aaaccgcggt ttctctatca ataaaaagaa agttcagcgc ctgatcaaga
     1981 agctaggaat aaaagtaacg acatacacac gtaaatcacg gcgctacaat tcttatcgag
     2041 gacaagt
//
LOCUS       DACEZN010000258         2044 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.268, whole genome shotgun sequence.
ACCESSION   DACEZN010000258 DACEZN010000000
VERSION     DACEZN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.268"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            80..1249
                     /locus_tag="IXH60_002812"
     CDS             80..1249
                     /locus_tag="IXH60_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1991686.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1516..>2044)
                     /locus_tag="IXH60_002813"
     CDS             complement(1516..>2044)
                     /locus_tag="IXH60_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991687.1"
                     /translation="RRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEE
                     FENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTLKSSKGKTIVLPVF
                     RYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIV
                     LLFYCIKLKIRTSHE"
BASE COUNT          727 a          342 c          307 g          668 t
ORIGIN      
        1 taaaatatat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
       61 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
      121 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
      181 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
      241 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
      301 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
      361 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
      421 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
      481 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
      541 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
      601 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
      661 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
      721 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
      781 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
      841 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
      901 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
      961 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     1021 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     1081 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     1141 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     1201 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     1261 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     1321 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     1381 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
     1441 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
     1501 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
     1561 aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa atgtgtatga
     1621 atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa aaggacctcc
     1681 ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt tataccggaa
     1741 tacaggtaaa actattgttt tgccttttga agatttaagc gtaaatgtaa tttcatcttt
     1801 atttggagaa aagctactat aaataacttg accatcttct gtaacttgat ggtttttcaa
     1861 aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa attcttcaag
     1921 tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt tcaccatgtt
     1981 aactactgat gaataattta aaattgaaat agctaatatt aataatagta taaaatttct
     2041 tctc
//
LOCUS       DACEZN010000259         2034 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.269, whole genome shotgun sequence.
ACCESSION   DACEZN010000259 DACEZN010000000
VERSION     DACEZN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.269"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IXH60_002814"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IXH60_002814"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1991688.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IXH60_002815"
     CDS             complement(1431..1823)
                     /locus_tag="IXH60_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1991689.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACEZN010000260         2019 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.270, whole genome shotgun sequence.
ACCESSION   DACEZN010000260 DACEZN010000000
VERSION     DACEZN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.270"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            99..569
                     /locus_tag="IXH60_002816"
     CDS             99..569
                     /locus_tag="IXH60_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1991690.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            791..1894
                     /locus_tag="IXH60_002817"
     CDS             791..1894
                     /locus_tag="IXH60_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1991691.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          459 g          546 t
ORIGIN      
        1 aatctctgaa gaataaatca cgtgatcgtg acgataaaat acacttacgt caggtgataa
       61 aaaatacata agtagaaaac taagaggagg aacagaaaat gaaagaagtt caattgatca
      121 gaaaaagcga actatcagaa ggtggttgca atgcatgcgg cgtagtagaa gcaacaagct
      181 atacattaaa gcttggtgcc aacaaagcga taatctctga gttgacagta ggcggtcttg
      241 tggacagtct ggcgcttgct gaaggtttta ttggagaaga tatttatgag atgttttcag
      301 aaatccgtca gttaaaaaaa ggggaaaact gcatagaggt tcatcatgag agcccgaatg
      361 tgagattcaa acgtggtgat aatgagatga tcttcaataa tcatgtgtcc gatcacaccg
      421 agctatatgg aatcgtgaat caaatactaa ctgagctttt tggattggga ccttatgcgt
      481 tcaaggaaga aaacggtaat ccaaaattaa acgaagaatg gcaagaaacg attgaaatac
      541 aaagaaacaa cccgcactta tttcaatagg catccacagt atttcgtaaa agggactttg
      601 acacgacttt tgtcaaaatc ccatcgtttt ttatctattt gtgttctaaa catttctttc
      661 agagtagtaa caactggaat gactgaaatg taaacagatt atttgcctgt atcaatacgg
      721 aagagtaata taaagtgtgt aaacgctatc atattattca ttcaattttt tatagaggga
      781 aggcattgaa atggaaaaaa cgtatcaagc aggtacaaat gaaggaatag tggattttat
      841 caatatggaa gatttagaaa tagccgcttc acaagtgatt ccagctggtg gttatggtta
      901 catcagcagt ggtgctggcg acttatttac ttatcaagaa aatgaacggg cattcaatca
      961 tcagctgatt attcctcatg tattaagaga tgtagaatta cccgatacaa ctacacattt
     1021 tgacaaagaa acgctgactg caccaatcat catggcacca gttgctgctc atggcttagc
     1081 tcatgtgaag gcggagaaag cttctgcaaa aggtgtagca gattttggga cgatctatac
     1141 agcaagttct tacgcttctt gtacattaga agagataaga gaagcaggcg gggagaaagc
     1201 accgcaatgg tttcaatttt atatgagcaa agatgatggg atcaatttgg atattcttga
     1261 agtggccaag cgtaatggag caaaagcaat cgtattgaca gcggatgcaa cagttggcgg
     1321 aaaccgtgaa acagaccgca gaaatgggtt taccttccca ctgccaatgc cgatcgttca
     1381 ggcttatcaa tcaggagtag gccaaacgat ggatgctgtg tacaaatctt ccaaacagaa
     1441 gcttagtcca aaagatgtag aatttattgc tgctcattct gatttgccag tctatgtgaa
     1501 aggtgtccag tcagaagaag atgtctatcg ttcattagaa tctggtgctg gcggaatctg
     1561 ggtatctaat catggtggcc gtcaactaga tggtggtcca gcagcgtttg attcgttgca
     1621 atatgtggca gaagcagtag ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg
     1681 cggacagcat gttttcaaag caatcgcatc aggtgcagat cttgttgcta ttggcagacc
     1741 cgttatttat ggattgtctt taggaggaag tacaggagtc catcaagtgt ttgatttctt
     1801 taaaacagaa ttggaaatgg tcatgcaatt agctggaaca caaacagtag aagatatcaa
     1861 aaaaataaaa ttgcgtgaga atcgttttat ttaagaaaca aatgacgaaa aaaataggag
     1921 tgcggcagat gatgccgtac tcctattttt atagttcaag gatgtcacta atagaataat
     1981 ttgaaacgaa aaaataaaat tgaaatattt tttcatact
//
LOCUS       DACEZN010000261         2003 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.271, whole genome shotgun sequence.
ACCESSION   DACEZN010000261 DACEZN010000000
VERSION     DACEZN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.271"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            316..801
                     /locus_tag="IXH60_002818"
     CDS             316..801
                     /locus_tag="IXH60_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1991692.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            794..1477
                     /locus_tag="IXH60_002819"
     CDS             794..1477
                     /locus_tag="IXH60_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1991693.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1603..>2003
                     /locus_tag="IXH60_002820"
     CDS             1603..>2003
                     /locus_tag="IXH60_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1991694.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVV"
BASE COUNT          621 a          343 c          428 g          611 t
ORIGIN      
        1 gtactgtaat acaattactg cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa
       61 atcttgccaa gccccctttt ttgcaactta tgataaacgg tgttcaaaag cttgcatctg
      121 cggtaataag cccaatcaaa tcaaaaacat gagaagctgt tttttcttgg ctgttcttat
      181 tatttgatgc aggacagctt tttcacgacc tctgtaaact ataggaatct gttaaatcaa
      241 gggagcagaa cttgcctttt acgctacttt tggtacaata atgtatgatt aaaaataaac
      301 tggagggttt ctatcttgaa cataggtaag ccgagaaaac taggaaaaac aataaatgaa
      361 atcgaggaag gcgactcgtt atccttaaca gaatctattg aagataacca gcttttgctg
      421 tatttgggat tgacgaatga tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa
      481 acagaatatc aacagccaat cgttccatca gtgatgctga tggggatcat cacaagtgca
      541 atttcaaagc atcttcctgg accaggatca catgttgtca atttttctgt caattttatt
      601 gaaccggttt tccattatga aacactgact tttcagtttg aggtcataaa agtagataag
      661 atgaaggatg tggtgacgat ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg
      721 gatgctgtcg ttatggtcca accgccacaa gttcctgaat tggaaatagc aatcgaaagt
      781 gaaggtgaat tgaatgaata atccaagtac atcaatgtca ggtatcaatc gtttttatgc
      841 aaaagtttat ggatttttag gtatgggact tgcccttagt gcagtcactt cttacttggc
      901 tctttgggta tttcctttgc aattagcaac ttttatcaat aattttccac taggctttgt
      961 gggtctgtgg atcgttgaac tgattttagt aatggttcta ggcgtaaaag cacaaaagaa
     1021 tccgagctta gcaatcggtg gatttattgc ctactctctt ttgaatggca taacattagc
     1081 ggttactctt gcatactacg atattggaac agtaacgcaa gctttcttga cagctacttt
     1141 gatgttcatg gggatgtctg ttgtcggagt aacaacaaaa agagatctta gcggggttgg
     1201 aagagcagga caggcagctt taatcggagt gatcattgca ctagtcttga acatcttcat
     1261 tttgagaagt agcgtagttg agctgttcat ctccctactg ttagtcgtca tctttgcagg
     1321 gattacagct tatgataacc aaagcatccg taatatctat gtccagacaa acggaacagc
     1381 acatagtgga attgctgtat tcatggcatt gaagctgtat ttggacttta tcaacttgtt
     1441 cttggcgttt ctgagaatct ttggacggaa caactaaaaa agaaaaaaat tcatcagttg
     1501 gggctatacc gaattatttg gtatggtccc gctttttctt ttcgcattcc gcaggattct
     1561 ttgttagaat gaataagaac tttacagtaa ggagttaaga agatgacaaa aaataaatta
     1621 ttactcgttg atgggaacag tgtggctttt cgcgcattct ttgcgcttca caattctctt
     1681 gaacgtttca aaaatagaaa cggtcttcat acaaatgcaa tctatgcatt caatacgatg
     1741 tttgaaaatg ttatgcaaaa agagcaaccg actcatgttc tggttgcgtt cgatgcagga
     1801 aaaacgactt ttcggacaga aatgtatgcg gagtacaaag gtggaagatc caaaacacct
     1861 ggcgaattca aagaacaaat gccatatata cgtgatttgt tgacaggact aggggtacag
     1921 tattacgaat tgcccaatta tgaagcagat gatattatcg gtactttggc agaaaaagta
     1981 gacaaagatc aatttgatgt cgt
//
LOCUS       DACEZN010000262         2001 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.272, whole genome shotgun sequence.
ACCESSION   DACEZN010000262 DACEZN010000000
VERSION     DACEZN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.272"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..804
                     /locus_tag="IXH60_002821"
     CDS             <1..804
                     /locus_tag="IXH60_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1991695.1"
                     /translation="HREQETILRELKKEFSQNKFSHFLDLLIDAGLIKREERRYHLNF
                     PIFDPKDYLQQATSAAETIAEQLKRLSVDEQKLAMGEVIWAYCFEDERKEAYFYGVRN
                     SRETELLRTTAGNEKYRFITLSSIEHFPLTLANYFFVQKNQLPVTKAFKELAELIGDV
                     NESYFFDQIEVIVDRIRKNKYKNRRPSIFHQSLLVTNTIKEEESFTLELPIVEKNNFE
                     IELPTLDPSLTMEEMAFLKRQIFSELSKKFIPHAFSYIKEYGTVLVSKT"
     gene            938..1063
                     /locus_tag="IXH60_002822"
     CDS             938..1063
                     /locus_tag="IXH60_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991696.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1197..1829)
                     /locus_tag="IXH60_002823"
     CDS             complement(1197..1829)
                     /locus_tag="IXH60_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991697.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT          766 a          290 c          369 g          576 t
ORIGIN      
        1 catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca aaataaattt
       61 tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga acgaagatat
      121 catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac ttcggctgct
      181 gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt agcgatggga
      241 gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt ttatggagta
      301 agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa gtaccgtttt
      361 atcacacttt cctctataga acacttcccg cttactttgg ctaattactt ttttgtacag
      421 aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat tggagacgta
      481 aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg taagaataaa
      541 tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa tactataaag
      601 gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt tgagattgaa
      661 ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa acgacaaatc
      721 ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa agaatatgga
      781 acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg actcttgcag
      841 gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg cctatctacc
      901 acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg gtgagaaatg
      961 tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc aatattatga
     1021 ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga agtccatttg
     1081 ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc aatcttgggt
     1141 tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg taatagtcag
     1201 gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac acctagactg
     1261 acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg gttggtcagt
     1321 ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt gacaggaact
     1381 aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat aaaataagta
     1441 aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt attgtgatta
     1501 aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa tacgcttgaa
     1561 gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata aacagaaaac
     1621 agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc ttctgtgata
     1681 tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga ggtcttaagg
     1741 tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc taaatgtaaa
     1801 aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga taagtatata
     1861 aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat aataaataaa
     1921 aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt tgtgagttat
     1981 tttccttgtc gttttctcac t
//
LOCUS       DACEZN010000263         1954 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.273, whole genome shotgun sequence.
ACCESSION   DACEZN010000263 DACEZN010000000
VERSION     DACEZN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.273"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..225
                     /locus_tag="IXH60_002824"
     CDS             <1..225
                     /locus_tag="IXH60_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1991698.1"
                     /translation="FVRSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDT
                     YNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            720..1319
                     /locus_tag="IXH60_002825"
     CDS             720..1319
                     /locus_tag="IXH60_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1991699.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1383..>1954
                     /locus_tag="IXH60_002826"
     CDS             1383..>1954
                     /locus_tag="IXH60_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991700.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          705 a          310 c          369 g          570 t
ORIGIN      
        1 ttcgtacgaa gcatttactc aactaattta atcgaatctt tcaataaaca aatcaagaaa
       61 tacagccacc gcaaggaaca gttccaaaat gaagagtcga tggaacgttt cttagtctcg
      121 tcttttgata cttacaacca aaaattccta ggtcgcagtc ataaaggttt ccaacaggcc
      181 gaaggcgagc tcgaacaaat gctgagtcaa ccgatggaga attagaaaca atctacagta
      241 gggaagaacc attgacacaa aattattgac gctcccaact aaaagtgtca aaataatgat
      301 gaaatcattt agttcttata aattagaaaa gagaaaataa atattccata tctactttat
      361 tggatccgta acatgactaa attctgaata ctttttaaag cttatcattt ggtaaaactg
      421 aatataagtt aattatatag actgaaaatt ttgaattttc agaatagaat aacttaaaag
      481 tactgacaaa attttttaaa ggggcaatat taatgttctg agataatcaa atcctaccga
      541 aaatatcaaa aatccaatgt gacacaatca gattgtgtct cattcgaggg aatcacacaa
      601 tcccacgtgg ctctaggcag aattgaattt ttgcttgggg ctttttcctt atattgattt
      661 tgaatgagac ttattatatg ttataatgca attaagtatc attggagagg gcgaagaaaa
      721 tgaagacggt agaacctatt cgagacaaaa aaaagatcga tgcgatgaag gcaatcttgg
      781 cttcgggaaa atatggccaa cgaaatttgg ttcttttttc gattgggatc aacaccgcct
      841 atcgcatttc tgatttgcgt caattgaagc tggccgacgt gctggaaatt tcacgcggtc
      901 gcgtggtggt caaagatcga ttagcgatga aggaacaaaa aacagctaaa cataattcgg
      961 tctatatttc caacaaattg cgaaaaacga ttttggagta cgttcaacag gaatttcctg
     1021 atcaactcct tcagcaagat tttgacggtt atttgttccc tagtcgaaaa gggacgaatg
     1081 aaccgctcaa ccgccaaagt ctgtggcgaa tcattcatga tgctggcacg gcaatcggat
     1141 tgaaagacat tggcccacat tccatgcgaa aaacgttcgg ttactttctc tataaacagg
     1201 ggaccaaaac agaaatcatt cagtccttgt tgaaccattc ttctcagcgc gaaacattgc
     1261 ggtatattgg tattacacaa gaagacaaag atacagctgt aaaaagctta gatttataat
     1321 ataaaagtta agtttaaaca tggatagttt taaattagta gattgagtgt tgggaggaaa
     1381 gaatgagtac attatatatt ataggaaacg gttttgattt aagtcatggt ttaaaaacta
     1441 gatacactga tttttcaaaa tgggttgaaa aaagtcatag tgaaatattc gataaaatag
     1501 acacaatgat aaatgaaaat aatgaggtga gaagtacagc tgatgataag ataggttggt
     1561 ttaattttga aagtagtttg catgagttaa ttcaaagtcc agtctttgaa gattatgtag
     1621 aagagtctaa ggattatctc ataggttatg gacatgaaga ttggtcagat tctgcccatc
     1681 atgattttca aaatatggtt tatgattatg ttgtttctct agaagaatta caacaacttt
     1741 tcaaggaatg gatacgcaaa gaaatacagc cgacaataga acataaaatt ccaaaatacc
     1801 aattttcgtc cgatgtacta ttctttacat tcaattatac agatgttttg gaaattctat
     1861 acaagcaacc gaaagaaaac atatatcata ttcatggcag ttatgacgat ataataatcg
     1921 gccactccga gaattttgac aactttatag gaga
//
LOCUS       DACEZN010000264         1845 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.275, whole genome shotgun sequence.
ACCESSION   DACEZN010000264 DACEZN010000000
VERSION     DACEZN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.275"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..302)
                     /locus_tag="IXH60_002827"
     CDS             complement(<1..302)
                     /locus_tag="IXH60_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1991701.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            661..915
                     /locus_tag="IXH60_002828"
     CDS             661..915
                     /locus_tag="IXH60_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1991702.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            916..1233
                     /locus_tag="IXH60_002829"
     CDS             916..1233
                     /locus_tag="IXH60_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1991703.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          638 a          279 c          347 g          581 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatg gatgttaacg
      481 gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt tttcagcgtt
      541 ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga aacaccgttg
      601 gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga gtgtgataac
      661 atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat ccttccaagc
      721 tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa cttattcata
      781 ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga aagtttccaa
      841 gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt cggaaaatac
      901 ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt tcagaccagc
      961 cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc cagcataaaa
     1021 agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc aactatgcat
     1081 gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt gatcggcaaa
     1141 agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt aggacgtatt
     1201 ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag tcgctcatcc
     1261 tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt actcgaagat
     1321 tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa ggtctaaaga
     1381 tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat cgcttggtga
     1441 ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat tcccaaaaat
     1501 ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat tgacgtaact
     1561 tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa gaggaagtcc
     1621 aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat acttccctgc
     1681 tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa tgggataaaa
     1741 tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat agtgaacata
     1801 cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atatt
//
LOCUS       DACEZN010000265         1842 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.276, whole genome shotgun sequence.
ACCESSION   DACEZN010000265 DACEZN010000000
VERSION     DACEZN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.276"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>1842
                     /locus_tag="IXH60_002830"
     CDS             <1..>1842
                     /locus_tag="IXH60_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991704.1"
                     /translation="VENSNNEFVATKFSDNFLSTFSDSLKSGYYRYQANLWGISIGLT
                     ALAIAYVFSAFVIITAILELAFKRVLGVLVFATDIETGQRSKVVLSDILQCYLTVGFQ
                     GFGLSMFAMFINFLNAGQGISTNIFIKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEG
                     YGQLASAFGMGAMLFRKGSTGFNRAKGSGNGNGSDSGEGEDRKPEKNFGETLSKKAGK
                     TGRALGYAHERGLSGLASDGATMASERAIKPFKSMRDMANDTKNKFKEGLDDGTVSAI
                     NKNSKPMLAKNKEDETGKYADSMPTRLSDRKDGAKVENADRIMSSSERMREAMKNNAE
                     SNNNPVSAIQQKVQQDIEERKNAMHGQAKSAEELINQKRQEAKYTPEAMNREEMLRKR
                     VEGRTGANTDEKEALTKERIQEAKNSNTGLEKLVKENLQNSTSSQGGRSVDVRENIQG
                     SLNGHVGRNVDVRENLQSSSNGQGTKTIDVRENVQNSTSSQGFKSMDVRENVQNSTSS
                     QGFKSMDVRENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDS
                     NHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDNLL"
BASE COUNT          686 a          254 c          403 g          499 t
ORIGIN      
        1 tagttgaaaa ttctaataat gaatttgttg ccacaaagtt ttctgataac ttcttatcaa
       61 cattttctga tagcttgaaa tcaggttatt atcgatatca agcaaatctt tgggggattt
      121 cgattgggtt gacagcgttg gcaatcgcct atgtgttcag cgcgtttgtg atcattacag
      181 caatattaga acttgcattt aaacgtgtgt taggtgttct tgtttttgcg acagatattg
      241 aaacaggaca acgttcaaaa gttgttttat ccgatatttt acaatgttac ttaacagttg
      301 gatttcaagg gtttggacta tctatgtttg ctatgtttat caatttttta aatgctggac
      361 aaggtatctc cacaaatatt tttataaaaa caattgctta tatctgtgct gtgtttgtgc
      421 tgataaaagg cagtggaaca gtcatgagat actttggtgt tgatattgga ctcaaagaag
      481 gctatgggca acttgcttct gcttttggta tgggcgcaat gctgtttaga aaaggatcaa
      541 ctggatttaa tcgtgctaaa ggaagcggaa atggaaatgg atcagacagt ggagaaggag
      601 aagatcgaaa accagaaaag aatttcggcg aaactctatc aaaaaaagct ggcaaaactg
      661 gaagagcttt gggatatgcc catgaacgtg gattatcagg tttagcttca gatggagcta
      721 caatggctag tgaaagagca ataaaaccat ttaagagtat gcgtgatatg gctaatgata
      781 ccaagaataa gttcaaagaa ggtttagatg atggtacagt gtccgctatt aataaaaata
      841 gcaaacctat gttagctaaa aataaagaag atgaaactgg aaaatatgct gattccatgc
      901 caacacgtct ttcggataga aaagatggtg ctaaggttga aaatgccgat agaattatgt
      961 ctagttctga acgtatgcgt gaagcaatga aaaataatgc tgaatcgaat aataatcctg
     1021 taagtgcaat tcagcaaaaa gttcaacaag atattgaaga acgtaaaaat gctatgcacg
     1081 gtcaagctaa gtctgctgaa gaattaatta atcagaagcg acaagaagct aaatacacgc
     1141 ctgaagcaat gaatcgtgag gaaatgttaa ggaaacgtgt agaagggcgt acaggagcta
     1201 atacggacga aaaagaagcg ttgaccaaag aaagaatcca agaagctaaa aactcaaata
     1261 ccggattaga aaaattggtc aaagaaaatc tacaaaattc tacaagtagt caaggtggcc
     1321 gatcagtaga tgtccgagaa aatattcaag gttcattgaa tggtcatgtc ggacggaatg
     1381 ttgatgtaag agagaattta caaagctctt caaatggtca aggtactaag acaattgatg
     1441 ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt taaatcaatg gatgtaagag
     1501 agaatgtaca aaattctaca agtagtcaag ggtttaaatc aatggatgta agagagaatg
     1561 tacaaagttc tacaagtagt caaggattca aaacagtaga tgttcgggag aacgttcaaa
     1621 aagatagtgg agaaatgaaa gaaaaaacac aaaaaatcaa tatcgttgaa gacagaaagt
     1681 cagcgaaaaa atttgattca aatcatgaaa cagtgattat tgatagtgaa atccgtgaaa
     1741 atgataaggg atcaaaacca agacgaagat ttacctatga agacaacgag ttattcagag
     1801 atacacttaa tgatcctaat ccgcttttcg ataatctatt aa
//
LOCUS       DACEZN010000266         1832 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.277, whole genome shotgun sequence.
ACCESSION   DACEZN010000266 DACEZN010000000
VERSION     DACEZN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.277"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            553..885
                     /locus_tag="IXH60_002831"
     CDS             553..885
                     /locus_tag="IXH60_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1991705.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1476..1805
                     /locus_tag="IXH60_002832"
     CDS             1476..1805
                     /locus_tag="IXH60_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991706.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          630 a          262 c          379 g          561 t
ORIGIN      
        1 ctgatattac ttcttttcag gcagtgctgc taatattcaa ttacactttt ttgtataaat
       61 ttagttgttt taaatttaat tgtctgctat gcttgtgatg aattgatttt cgttctgcac
      121 ttttggacga taaatttgga caattgaaaa tacaaaaaaa agaaaggccc tgtgagactc
      181 ggaaggctac caaccttcgt cgacgtccac aggtggcatt aagtctatac cccttaatgc
      241 gtttaccttt cagtgttacg gtattacccg taacggattt agtataacag atattcaaaa
      301 gtgataccac tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta
      361 tgcctaagca taatgttata aaaatgtccg tggggctctt tgccgtatga cgaacgctga
      421 aattagaggg aacgcatgtt tgaatgcgtt ccctcttttt tgtttgtcat gaacgaaaat
      481 caattctcta tttttgaaaa ataacaagaa aaacttgtta aaaatattta aagtaagtgg
      541 gtggattatt ttatgtcata tacaggaatt ttgagtctcg aagatatttg tcactacggt
      601 aaacggtgta ccgctacaga aaagatcacg aagaaactat caactggtca aagtaaaacc
      661 gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagaaga aatgatttat
      721 tacattggga aacaaaaaca aataatttta aaagatccta tccctttaaa agagctatat
      781 ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga
      841 gtcttgcgtt gtaccgcaaa agggatgggg cgtttaatca gctgaattga tcattctcat
      901 aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaggaact
      961 tagaatcact agaaaaataa ggagtagact tgatctatca acaattccct tcggggagtg
     1021 acggggtagg tttttacgat tggtaacgta aaaaattggt caatcgagta aatgcccttg
     1081 tttggatagt ggtgaaatga atttgtgtaa ataatatgca cagagtaaac aagagggaat
     1141 ttagaagtta gacaagttag aatagcgcct agattaatca tgagcagcta ctaagggatc
     1201 taggctttga cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca
     1261 caagaaattg tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tgatcattgg
     1321 atcaatcgag catttaccgc tgtctactag ggtttctcgt ttcggacgcc ataaacgagt
     1381 cgatttattt tatatatttt tgagtgaacg aaaaacgata aagatattcg ttatgaagta
     1441 aaactaattt agtagatagc aaaggtggta gaggaatgat tcaaaaattt gcgaaaatga
     1501 gtaaaactgc atggattgaa aagcttgaaa atgacttgag tgtcatgcga ttagctccaa
     1561 agaaattcaa acgagagatt gcagaaaaag aagcaatttt gtcggtattg aatgcggtag
     1621 acgataaatg tccagtaaaa attagttttt cagatatagc aacaattatc gaaggtattt
     1681 attatggtga agttgatgaa gagatatctt ctacaaaaga tttattagat tttttggata
     1741 aaaacaaaga tacctttata gtactaactg attatgagga ggttacaaca aatgaaaaat
     1801 tatgatccga atatacgatt gggcacacat ac
//
LOCUS       DACEZN010000267         1825 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.278, whole genome shotgun sequence.
ACCESSION   DACEZN010000267 DACEZN010000000
VERSION     DACEZN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.278"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(51..1517)
                     /locus_tag="IXH60_002833"
     CDS             complement(51..1517)
                     /locus_tag="IXH60_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1991707.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          623 a          360 c          315 g          527 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tactt
//
LOCUS       DACEZN010000268         1818 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.279, whole genome shotgun sequence.
ACCESSION   DACEZN010000268 DACEZN010000000
VERSION     DACEZN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.279"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..325
                     /locus_tag="IXH60_002834"
     CDS             <1..325
                     /locus_tag="IXH60_002834"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1991708.1"
                     /translation="IVVPKTLPKRQMAFYEMDEQSVLVRSSFGVLEPQSEKLYLPEMI
                     DLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQLNEQWQPESFDQKIQ
                     QLFID"
     gene            345..1043
                     /locus_tag="IXH60_002835"
     CDS             345..1043
                     /locus_tag="IXH60_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1991709.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            1064..1276
                     /locus_tag="IXH60_002836"
     CDS             1064..1276
                     /locus_tag="IXH60_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1991710.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            1276..>1818
                     /locus_tag="IXH60_002837"
     CDS             1276..>1818
                     /locus_tag="IXH60_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1991711.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKR"
BASE COUNT          528 a          289 c          432 g          569 t
ORIGIN      
        1 aatagttgta cctaaaactt tgccgaaacg tcagatggcg ttttatgaaa tggatgaaca
       61 gtccgttctt gtacgttcga gttttggtgt tcttgagcca caatcagaga agctctattt
      121 gccggagatg atcgatctcc tcattgtacc aggtattatc tacaatagag ccggttatcg
      181 aatcggtttt ggcggtggtt attacgaccg gtatttaaat aattttcata aaaaaacatg
      241 tagccttgtt tttcgtgaac aattgaatga acaatggcaa ccggagagct ttgatcagaa
      301 gatccaacaa ttgtttattg actaaaatga aatgggaggt tttcatgaat tatcaacagc
      361 aaataaaatt gcgtgcatgg ttaagacgac cgttatggac ctatgctttt ttagggatac
      421 agacagcggt cttcctcatg atggaattat ttccgcgact tgagatccct tattatgccg
      481 gaatgtatgg tccttatctt gttcacttca atgaatggtg gcgtttagtg acaccaatct
      541 tcatccattt tggactgatg catttcgtaa tgaactcact gatactgtat tttatggggc
      601 agcaaatcga ggcaatctat ggccattggc gttttttctt gatttatatg ttcagcggga
      661 taatgggaaa tacagcaagc tttgctttta acgaagcgaa tgttctgtct ggtggtgcaa
      721 gtacttcgat attcggtttg ttcggtgcgt tgtttatctt agggtttcat tttaaacata
      781 acccagcgat ccagcagcta gtcagacatt ttcttttatt tattgtgttg acatttgttt
      841 ttggtcttct ggatacctct gtagatgttt ggggacatgt cggtgggcta attggcggat
      901 tagtgttagg gaatgttttg ggtctaccga aacaacagac aagctattcg atacaccaaa
      961 ggattctttc tacactagtt tttgtatttc tttttgtaat atgtatttta ctgggtttaa
     1021 aaaaatatgg tttacttgta taatatgaag gaagtgctta aagatggaga gcttatatga
     1081 tgtacagcag ctgttcaaaa aatttggcat ctttatctat gtgggtgcac gcatctatga
     1141 cattgaatta atgactatcg aattgcgtaa tctgtatgat agccgtttga ttgatcatga
     1201 tacatacatg acagcgtggc gcatattaaa aagagagcac cgaattgaag aaagccgaaa
     1261 gaaaggaaat ttgtaatgga caagaagatt attgggattg atttaggtgg aacaacagca
     1321 aaatttgcaa ttttaacacc agaaggagag attcagcaaa aatggagcat cgataccaat
     1381 attttggatg atggtaagca tatcgttcct gaaatcattg aatcaatcaa tcatcgtttg
     1441 aatttatata atatgaaagc cgaagacttt atcggaatcg gcatgggtac cccaggaagc
     1501 gtggacagcg aagctggaac ggttattggt gcgtataact taaactggac agaagttcaa
     1561 tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat tcgctatcga taatgacgcg
     1621 aatgttgctg ctttaggtga acgatggaaa ggtgctggag aaaacgatcc agacgttgta
     1681 ttcgttactc taggaacagg tgttggcgga ggtatcgttg ctggcggaaa tttgattcac
     1741 ggcgtgagcg gagctggagg agaaatcgga catatcactg tggatcctga agggttcgaa
     1801 tgtacttgcg gtaaacgc
//
LOCUS       DACEZN010000269         1799 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.281, whole genome shotgun sequence.
ACCESSION   DACEZN010000269 DACEZN010000000
VERSION     DACEZN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.281"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1029
                     /locus_tag="IXH60_002838"
     CDS             <1..1029
                     /locus_tag="IXH60_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1991712.1"
                     /translation="SFVISYIQSYFTINFNHFKYLILSFISTFLNIAISVALMQTLYF
                     DDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIV
                     LAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKV
                     KKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPV
                     NAEFYEKKTSFITIGTVASAIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFHYNIA
                     KKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTSLLIISLVIYFLKIKN
                     RIIDDYNS"
     gene            1042..>1799
                     /locus_tag="IXH60_002839"
     CDS             1042..>1799
                     /locus_tag="IXH60_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1991713.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRG"
BASE COUNT          629 a          222 c          307 g          641 t
ORIGIN      
        1 agttttgtaa ttagttatat ccagtcttat tttacgatta actttaacca ttttaaatat
       61 ttgattttat cttttatatc tacttttttg aatattgcaa tctctgtagc tttgatgcag
      121 acattatatt ttgatgataa atatgttggc agagtagtcg gcggaacact gtcatttgtg
      181 ttgataggtt tagtaattta tttaagaata atgataaagg gtgctcattt ttttgataaa
      241 aaacagtgga gatacgcttt aaaaatctct ttgccgctaa tcccacattc cttagcaaat
      301 atagttttag cacaattcga tagaattatg attaattctt atttaggtcc tgctgcgggg
      361 ggaatatatt cctacattag taacataggt attattctaa gcgtaatatg gatgtctact
      421 aataacgcct gggttccatg gttctatggt gagatgagta aaaataatga aaaaaaagtg
      481 aagaaagtta gtaactatta tttagtagtc tttactttga ttacgatgat tgtaatggta
      541 gtttcgattg atttagcaaa atttatggct ccgaaagact attattctgg gttatcttta
      601 gtggttccga taaccttagg ctatttcttt cagtttttat atagtttacc agttaatgct
      661 gaattttatg agaaaaaaac atcgtttatt acgattggta cagtagcaag tgctatagtt
      721 aatatactat taaacatgat tttcatacct aggttaggaa taattgctgc tggatacaca
      781 accgtattta cgtatttttt actttttatt tttcattata atatcgctaa aaaaattacg
      841 aataaacaat tatttgacac aaaaaaaata tgtatcataa taatttttgt atcaattttt
      901 tctttgatac ttcatatttt aatcaatcaa atgttgattc gttacgtttt gacatcatta
      961 ttgataatta gtttagttat atatttttta aagattaaga atcgaattat agatgactat
     1021 aattcatagg aggagatata agtgtttaaa ggaaaaacaa tattagtaac tggcggaact
     1081 ggttccattg gaagtgaaat tgttcgtcag cttttaaaat atgaaccaaa agctatcaga
     1141 atttatagta gaggtgaaga aaaacaattt tatatgcaac aagagctctc aaatcacact
     1201 aatttgagat ttttaattgg tgatatcaga gactctcaaa ggattgatta tgcatgtcgt
     1261 gatgtggata ttatttttca tgctgccgca atgaaacacg ttccagcaag tgaatataat
     1321 cctatggaag ctgtaaaaac aaatgtgatg ggaactcaaa atgtaataga tgcagcgatc
     1381 aaaaataatg taaaacattt tgtaggtatc agtacagata aagtagtgag tcctactaac
     1441 accatgggtg cgacaaagtt attgagtgaa aaattaataa tagctgccaa tcagtataaa
     1501 ggaaaaacag aaacaatatt ttcttgtgta aggtttggaa atgtcatggg gtcaagtgga
     1561 tctgttattc ctctttttag taaacaaata aaaaacaata aagatattac tttgacagat
     1621 gttgatatga cacgatttat gatgtcaata ccggatgctg caagattagt tctgtctgct
     1681 gctatgaatt caaaatttgg agaaacatat gtcttaaaaa tgccggcaat caatatttta
     1741 actttagctc aatcattaat aaaaatatat aatgatacac atgatgaaaa atatagagg
//
LOCUS       DACEZN010000270         1766 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.282, whole genome shotgun sequence.
ACCESSION   DACEZN010000270 DACEZN010000000
VERSION     DACEZN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.282"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..825)
                     /locus_tag="IXH60_002840"
     CDS             complement(<1..825)
                     /locus_tag="IXH60_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1991714.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEA"
     gene            complement(901..1293)
                     /locus_tag="IXH60_002841"
     CDS             complement(901..1293)
                     /locus_tag="IXH60_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1991715.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            complement(1274..>1766)
                     /locus_tag="IXH60_002842"
     CDS             complement(1274..>1766)
                     /locus_tag="IXH60_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991716.1"
                     /translation="SLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLSLKDALIVMS
                     LSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVCFFLAGMVYG
                     IGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFYNHSGGKNEK
                     NNL"
BASE COUNT          549 a          396 c          289 g          532 t
ORIGIN      
        1 agcttcattc tctttgttac caacgatatc tgcttttgct gcaactaaaa tctccgttgg
       61 acttaaatga actgttttta tatcgatcag ccgactgatc tcaggacgtt caaaagctaa
      121 cttgattttt gataaatcgc ttgctgtcac gctttcaccg atgatcagac tataaaattc
      181 ttttgctagg aaaatggcgg ctcccatcag caagaaaccg atcagcaatc cgctgatcgc
      241 atcaaatgca gcaatcccgg ttgcataagt caatattgtt cctgctaaag ccaagaccaa
      301 accgattaca gcacacaagt cttccgtaaa aataatcagt atttcactgt gtcgactttc
      361 gcgcaaaaag cgcatcgtgg aaagtttttc agtattcaat tctttgattt ctttcattgc
      421 cacgcgtaac gaacttcctt cgattaccaa accaaataac agtattgcta taacaatgat
      481 cggattaccc acttcatggg atggatgcgt caacttttca aaagcttcca tcaccccaag
      541 tgccccaccg ccgaagaaaa gcatcattgc gacgattgta ctgaaaaagt attttgctcg
      601 tccttcacca aattgatgca actcgctctg tcctttattg gcttgtttat caccgaataa
      661 cagcaaaatc tgattgctgc aatcaacgat actgtggatg ctttcgttta gcatggcggc
      721 actgccactg attgcatagc cgacaaattt gctgatagca actagaatat tagcccctag
      781 agcagcaatt acggaaaaca ttccgctttt tttaaaattt tccatgttat tttccttctt
      841 tcatcaactg aactcactga attatggcaa tattcgccct ttaacagttt tttatgaaac
      901 ttatagttta gacaaattga aaatgaccga ttgtccatca ttcaatttga tctctttggc
      961 accagtagta gcacttttcc cgtccacagt aaacatccaa tagagttgat ttttctcatc
     1021 ctgtgagtgc ccttcaagcg aagtgatgaa gccgttatct tctttgactt caaaatttcg
     1081 tttcaaaata ctcagtaaat catcgccttt tttgaagaac acttcttttt ccgaaaagtt
     1141 ttgaccattt tcttgtaacg tgattgttgc tttttgcgat gtttttactt cctccttttg
     1201 gttggaagcc gattgtgtat aggttgtact gcaacttgtc aaaatgagta tactgacact
     1261 cattgcaagc aaactataaa ttattttttt cattttttcc tcctgaatga ttgtaaaaac
     1321 gtttgacgac tggcagaaaa acaagaaaga acacaacatt gcctatactg tgcatcaaat
     1381 caaacgacac cccttgaagg taatagatcc aaggttgagg caaacgatat aacagcgtat
     1441 caaaaacggt catacctatt ccgtaaacca tgcccgccag aaaaaaacag actcccttta
     1501 accatagatt cctttgcact gtttttctca agcagacgat tgaaaacaaa ctcagaacaa
     1561 cagtatagct gatccattgg ccgatgaccc atggtccaac accaaaataa aaactgctga
     1621 tcgcaataga aagactcatg acaattaacg catcttttaa gcttaagtaa agtactataa
     1681 ataaaaaaat agcagtcatt ggctggaaat tcggaataaa ttgaaaagcg atcctacctg
     1741 ctacacaagc agcagacaat aagcta
//
LOCUS       DACEZN010000271         1750 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.283, whole genome shotgun sequence.
ACCESSION   DACEZN010000271 DACEZN010000000
VERSION     DACEZN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.283"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            383..1633
                     /locus_tag="IXH60_002843"
     CDS             383..1633
                     /locus_tag="IXH60_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1991717.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          577 a          335 c          309 g          529 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaatgacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
     1681 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
     1741 aatttcatct
//
LOCUS       DACEZN010000272         1745 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.284, whole genome shotgun sequence.
ACCESSION   DACEZN010000272 DACEZN010000000
VERSION     DACEZN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.284"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..150)
                     /locus_tag="IXH60_002844"
     CDS             complement(<1..150)
                     /locus_tag="IXH60_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1991718.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQS"
     gene            complement(554..853)
                     /locus_tag="IXH60_002845"
     CDS             complement(554..853)
                     /locus_tag="IXH60_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991719.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(859..1089)
                     /locus_tag="IXH60_002846"
     CDS             complement(859..1089)
                     /locus_tag="IXH60_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991720.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1339..1539)
                     /locus_tag="IXH60_002847"
     CDS             complement(1339..1539)
                     /locus_tag="IXH60_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1991721.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          594 a          286 c          268 g          597 t
ORIGIN      
        1 tgattgtgct tgcttcatcc atttatcgaa agaagaaggc gtaagctcat actctcgaat
       61 aatttctgcg cgaggcttac cagcgagata gagatcgacg atttgttgtt tgaattcttt
      121 tgaataggtt cttcgttgac gtcttgacat gaaaaatcct ccagtgtgtt ttttattatt
      181 ctacacacct tattttttct gtctagtcta gtgtagccga ttcagaaaga catttgggtt
      241 atattgtgag ataataataa agaagagttt aaagcgttcc ccaaaaacca ctgccccata
      301 agtgtgttac gctttaaact cttttatatt tgaagccatt aaaaagcata ccatattttt
      361 gaaaaaaagt gagaaaaaag gcttataatt ggagtggtag ttaattagtg acttattttt
      421 gattttatag cactgatact ataaaatata gatatcatca tattacacaa tcttaatact
      481 aacttaaaaa atatctcctt tcataagtat ggtgataaaa tccgttccgg gctacctttt
      541 taggtagcct actttaatct ttgtatcttt ctggatcaac gaaagtatac tttatatagt
      601 cataacgccg atgatcgctt cgagcgtcta gcacatcagt cacgatatca aacaaaaaat
      661 atacgtcttt cttcattcta gttttcgcag cagggatttt aaagtagttc ttattagaat
      721 agtagagatt aattaataag ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca
      781 ccttataaaa atctttgaca aatctattcg aaggatcaaa tttaaaccat aattgcgtct
      841 cattaaaaag cataaccatt actccgatct atttttaaac ttagtctcta cctctaatat
      901 atatcgagtt tttattttgc cttcagagaa tactgtttct ttttttgcag ttacaggttg
      961 tttattttcg gaaaaagcta atatagctaa aattgaaaca tccatctgga atttgtcttt
     1021 ttcgttgctt tgctcataaa aatctgcata ttcatcacta atattttttc taataaattc
     1081 ttccatcata aagatcacct cgaaaagagt atacgaacaa acgttctttt tgtaaaggtg
     1141 gaatttatta tactaaataa ataggtgaac aagtacttgt gccaaattat gtgccaaaaa
     1201 aaatcgaatt taataaaaca caaacaaaaa gaaatcctat tatgataaca tttcttataa
     1261 taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata acccctgtat
     1321 cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta aactgctatg
     1381 tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg
     1441 cgtatgtctt agtccatcaa taaacagtcg aagagaaaat agggaaaaat tgattactaa
     1501 agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaataga ttcgaattaa
     1561 acgatttcga ttggcttata ccagtcctat ccaacgaaag atatatcgtt cttaactcta
     1621 gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat gactttttat
     1681 tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa gtaccatata
     1741 aaaaa
//
LOCUS       DACEZN010000273         1733 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.285, whole genome shotgun sequence.
ACCESSION   DACEZN010000273 DACEZN010000000
VERSION     DACEZN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.285"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..107
                     /locus_tag="IXH60_002848"
     CDS             <1..107
                     /locus_tag="IXH60_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1991722.1"
                     /translation="MEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            162..1472
                     /locus_tag="IXH60_002849"
                     /pseudo
     CDS             162..1472
                     /locus_tag="IXH60_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
     gene            complement(1602..>1733)
                     /locus_tag="IXH60_002850"
     CDS             complement(1602..>1733)
                     /locus_tag="IXH60_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1991723.1"
                     /translation="RRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          703 a          165 c          300 g          565 t
ORIGIN      
        1 gcatggaagc catccgagga ttatataaga aaacccgaaa agaaggcact ctcttcgggt
       61 tttcggtctg tactgaaatc aaggtattat tgggaatccc agcttaaatc atagataccg
      121 taagggattt tattctttat ttaaaacttt gcaacagaac cgaatcatta aaaaaacatt
      181 atacagagcc ttgggaagat gaagttttta gagtaattat tgaatataac aatgttccta
      241 ttggatatgg acaaatatat aaaatgtatg atgagttata tactgattat cattatccaa
      301 aaactgatga gatagtctat ggtatggatc aatttatagg agagccaaat tattggagta
      361 aaggaattgg tacaagatat attaaattga tttttgaatt tttgaaaaaa gaaagaaatg
      421 ctaatgcagt tattttagac cctcataaaa ataatccaag agcaataagg gcataccaaa
      481 aatctggttt tagaattatt gaagatttgc cagaacatga attacacgag ggcaaaaaag
      541 aagattgtta tttaatggaa tatagatatg atgataatgc cacaaatgtt aaggcaatga
      601 aatatttaat tgagcattac tttgataatt tcaaagtaga tagtattgaa ataatcggta
      661 gtggttatga tagtgtggca tatttagtta ataatgaata catttttaaa acaaaattta
      721 gtactaataa gaaaaaaggt tatgcaaaag aaaaagcaat atataatttt ttaaatacaa
      781 atttagaaac taatgtaaaa attcctaata ttgaatattc gtatattagt gatgaattat
      841 ctatactagg ttataaagaa attaaaggaa cttttttaac accagaaatt tattctacta
      901 tgtcagaaga agaacaaaat ttgttaaaac gagatattgc cagtttttta agacaaatgc
      961 acggtttaga ttatacagat attagtgaat gtactattga taataaacaa aatgtattag
     1021 aagagtatat attgttgcgt gaaactattt ataatgattt aactgatata gaaaaagatt
     1081 atatagaaag ttttatggaa agactaaatg caacaacagt ttttgagggt aaaaagtgtt
     1141 tatgccataa tgattttagt tgtaatcatc tattgttaga tggcaataat agattaactg
     1201 gaataattga ttttggagat tctggaatta tagatgaata ttgtgatttt atatacttac
     1261 ttgaagatag tgaagaagaa ataggaacaa attttggaga agatatatta agaatgtatg
     1321 gaaatataga tattgagaaa gcaaaagaat atcaagatat agttgaagaa tattatccta
     1381 ttgaaactat tgtttatgga attaaaaata ttaaacagga atttatcgaa aatggtagaa
     1441 aagaaattta taaaaggact tataaagatt gattatataa tatatgaaaa gctattataa
     1501 aagacattag tattaaatag tttaaaaaaa tgaaaaataa taaaggaagt gagtcaagtc
     1561 cagactcctg tgtaaaatgc tatacaatgt ttttaccatt tctacttatc aaaattgatg
     1621 tattttcttg aagaataaat ccattcatca tgtaggtcca taagaacggc tccaattaag
     1681 cgattggctg atgtttgatt ggggaagatg cgaataatct tttctcttct gcg
//
LOCUS       DACEZN010000274         1724 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.286, whole genome shotgun sequence.
ACCESSION   DACEZN010000274 DACEZN010000000
VERSION     DACEZN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.286"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..679
                     /locus_tag="IXH60_002851"
     CDS             <1..679
                     /locus_tag="IXH60_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAQ1991724.1"
                     /translation="DDRIQRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSL
                     FNENNWVNCFYANTTFEIEFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQY
                     LERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKIL
                     LHTIDLYEETTELSALSASLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKI
                     LYAEVSK"
     gene            676..>1724
                     /locus_tag="IXH60_002852"
     CDS             676..>1724
                     /locus_tag="IXH60_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1991725.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYP"
BASE COUNT          671 a          227 c          293 g          533 t
ORIGIN      
        1 cgatgataga atacagagaa agtgtgctat tgtaactgat ttagatgaac aggcaatccc
       61 atcagaacat cgattgtata aaagcactgc acaagagaaa ggtcaagcaa gaaaagaaaa
      121 attatcctcc ctttttaatg aaaacaattg ggttaattgt ttttatgcta atacaacatt
      181 tgaaattgag tttttagatg tgaataaaga aaatattcta acttatgtca gtccaattat
      241 agatgacgca ttcaaagata aaaaaacaat aactgcttat aaagaagaaa tacaaagtga
      301 acaatattta gaaagaaatt tagcgatgtt aaaactagcc aatcatgttg gtaaggggtg
      361 gtttgcaatt gagttaggtg aaaaaattga ctttttaatc aaaattccta agtatattct
      421 taatgctatt gcttttgcat gtcaggaaat agtagatctc tcaatttata aaaaaattct
      481 actccataca atcgacttgt acgaggaaac tacagaattg tcagctttat cagctagttt
      541 aaaaacaagt gatacagctg atttgaatat agcaataatt tctttattag agtactttgg
      601 taaagatgag gatgttgtgt atctaatcaa tcagatagaa aattacacaa aaatacttta
      661 tgctgaggtt tcaaaatgac aaatatcttc gaaaaaatcg taaatgattt aaatttagaa
      721 caaaaagatg ttgtggaaag tgaagataac ctttatgtaa atgcatgtcc tgggagtggg
      781 aaaacaaggg tacttacaag gaaaattgct taccaagcaa ttaaacacga tggatcacta
      841 aaaagaataa ttgctatcac atacactaat agagctgctg aagaaattaa ggaacgccta
      901 agtttactta gtatagatga tgatattaat atttgggtgg ggacaataca tcagttttgt
      961 cttgaattta ttatttatcc ttttaaaatg aatctctcaa gagtatcaaa aggatttaca
     1021 attattgatg attatactca aagagcttac gtcagccaaa ttaataatat gctgaattta
     1081 aatctcaaaa cgtatgaact gaacaaaatt gatttgtcac tttctcatga ttttaaaatt
     1141 aatgaagttc agtatactca agtttctgaa atgtatcaca ctatgttgga aaaaaataaa
     1201 gaaattgatt ttgatatgat tttactagta gcgtataaaa ttttaactgt aaacagtaac
     1261 gttgcacgta atattgctag taatataagg gcgatttatg tggatgaatt tcaagatact
     1321 agagaattac aatacaatgt aatagctaaa ttacttcaaa acaaccctca aattcagtca
     1381 atgtttgttg gagatattga ccaagcaatt tatggatcat tagatggtat agctaaatca
     1441 gttgaagaac tagaaatgct aactagtcac acttttcaat caaagacgct acatggctgt
     1501 tacaggtcta atcagagatt agtagacttc tactctaatt ttcagagttg tccttacgag
     1561 attgaaagca gagggaataa taaaaacgac cgtggtttta taagttatga ttgcaaagtt
     1621 actaaggagg atttacccaa cataataaaa aatataataa aggaaaagat tgaaagcggc
     1681 attcatgaaa aagatatttg tgtacttgct cctcaacact atcc
//
LOCUS       DACEZN010000275         1709 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.287, whole genome shotgun sequence.
ACCESSION   DACEZN010000275 DACEZN010000000
VERSION     DACEZN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.287"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            287..694
                     /locus_tag="IXH60_002853"
     CDS             287..694
                     /locus_tag="IXH60_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991726.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IXH60_002854"
     CDS             708..1109
                     /locus_tag="IXH60_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991727.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IXH60_002855"
     CDS             1111..1482
                     /locus_tag="IXH60_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991728.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..>1709)
                     /locus_tag="IXH60_002856"
     CDS             complement(1518..>1709)
                     /locus_tag="IXH60_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991729.1"
                     /translation="DSLLINLYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSD
                     HRRYDYIKYTFVDPERYKD"
BASE COUNT          637 a          221 c          296 g          555 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aaattattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatc
//
LOCUS       DACEZN010000276         1679 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.288, whole genome shotgun sequence.
ACCESSION   DACEZN010000276 DACEZN010000000
VERSION     DACEZN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.288"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(135..1568)
                     /locus_tag="IXH60_002857"
     CDS             complement(135..1568)
                     /locus_tag="IXH60_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ1991730.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            complement(1561..>1679)
                     /locus_tag="IXH60_002858"
     CDS             complement(1561..>1679)
                     /locus_tag="IXH60_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1991731.1"
                     /translation="DQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          653 a          280 c          186 g          560 t
ORIGIN      
        1 acgctcacta tatcttctag cgttttcaaa ttcagaataa ataaataaat ctccttttga
       61 ttctcggtaa aaattttgat aaacatttaa tatattaatc aaatgtaaag gagtccaagc
      121 aatgaaagct gtcttcatat taattctcct ttttttaaat acaagtaaga aatgcttccc
      181 aaataatacc agaaaacaat tgaaatgaca tcgcgcttat tgaaaagtac atgagcctcc
      241 accatttcat ctataaaaat agatagtata aaaattaaaa caataccaag aaacgcataa
      301 ttcactttac ttctaaaaaa tgttttgagt aaactaaaga tatttaacac atagaaagca
      361 tagatacata taaaagcaat tataccactt gatacaagta cttgaagaaa tccattatgc
      421 atgtttccat gtgctctttt tagttcattc acatctgtag tatcaagatc tacttttttc
      481 aaaaattttg catcgctatt cccatagaaa tcggtctctc cataaccaaa taacaaagtt
      541 ggtcttataa ctttaagcgc agctttccaa atcgtaaaac gaccagctga aatttcggag
      601 ttatcactag aatgctgtaa tattacttct ttttctggaa gtggagtttt atctgattca
      661 tttgactcta taacttctac ttttctatca atcgatacag gaattgttgc taaaaaatcc
      721 ttagcataat tagtaccaaa agaaaaatat accaatagaa ctaataatat gcttaccatc
      781 catttgattt ttacaggtag tacttctcta atgctctttc taaattttga attggcaaac
      841 aacaaaagaa ataatactaa aaaagaacaa attactacct gggcacctct agacgaagaa
      901 agtgtaatat aaaatatttg tacgataaaa ttcatcatat aaaaaacacg aatcgttttt
      961 gttatttttg aaattaatac actaattaaa cttaaaataa tagaagtata tccaaacata
     1021 gctccaacat ttggacttgt atagacacca aataaccgat gttcaataaa accttgtctt
     1081 gcaagtaatc ctggcgttcc tgttgggaca tgataagaaa tcaaaaaaat aaattgatag
     1141 atagatatta gcgcagctaa aaaaattatg ataatgaaaa cattattaaa atacaccatt
     1201 tttttctttt tttcctcttc actttgtgaa aagtctgcag gaaaaattac tatcaaagta
     1261 atcagcaaat atccaaaatt ataaatagat ggaattaaaa aattttctct gttaatgatt
     1321 atagttaaga taaaactaat cattgctcca atcagtagcc aagaaaaacg cattttaaaa
     1381 aaatttttct ttataaaaac ctcatacaaa actataattc caccccaagc aaaagctatt
     1441 ttcatcggga tattagttac attaaaagta ctaggcataa aataaagcag acaaatgatt
     1501 aaatttatta ctcgaaacgc atctcgactt aataaactct tctctaaaac aggtactatt
     1561 ctattcatct atatttccaa ctttcaaaat ttctttatca attctattta atagtttatc
     1621 agagtaatcc gatttaactt ttcttacaaa taatttttca ctttctttta tttgatcta
//
LOCUS       DACEZN010000277         1678 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.289, whole genome shotgun sequence.
ACCESSION   DACEZN010000277 DACEZN010000000
VERSION     DACEZN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.289"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(365..1657)
                     /locus_tag="IXH60_002859"
     CDS             complement(365..1657)
                     /locus_tag="IXH60_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1991732.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          516 a          343 c          284 g          535 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatct
//
LOCUS       DACEZN010000278         1670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.290, whole genome shotgun sequence.
ACCESSION   DACEZN010000278 DACEZN010000000
VERSION     DACEZN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.290"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1565
                     /locus_tag="IXH60_002860"
     CDS             <1..1565
                     /locus_tag="IXH60_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1991733.1"
                     /translation="VTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNR
                     SDADKYTPMVEGEKVEIGGTVDLTDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEG
                     VIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPTLP
                     EGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEG
                     QKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDE
                     VEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDV
                     STAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAE
                     VTVTLPDGSQYHGTADKNGNFTVKVPKLEAGTKVRVTATDESGNTSEPTNVVVSSNKK
                     DSGKNGSKGSKTDNQDGNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLG
                     LLGYKNRKKENE"
BASE COUNT          659 a          257 c          363 g          391 t
ORIGIN      
        1 cagtaacaga tgttactcct ggcggtacaa ttgataccaa cacaccaggc aattatgaag
       61 gtgtcattga agtaacgtat ccagacggta cgaaagatac agtgaaagtt ccagtagaag
      121 tgacagataa tcgttctgac gccgataaat atacaccaat ggtagaaggc gaaaaagtag
      181 aaattggagg cacagtagat ttaacagata atgttactaa tttgccaaca ttaccagaag
      241 gaacaacagt aacagatgtt actcctggcg gtacaattga taccaataca ccaggcaatt
      301 atgaaggtgt cattgaagta acgtatccag acggtacgaa agatacagtg aaagttccag
      361 tagaagtgac agataatcgt tctgacgccg ataaatatga accaacagta gaaggcgaaa
      421 aagtagaaat tggaggcaca gtagatttaa cagataatgt tactaatttg ccaacattac
      481 cagaaggaac aacagtaaca gatgttactc ctggcggtac aattgatacc aacacaccag
      541 gcaattacga aggtgtcatt gaagtaacgt atccagacgg tacgaaagat acagtgaaag
      601 ttccagtaga agtgacagac aatcgttcta atgcagataa atacaatcct gaaggacaaa
      661 aagtgacgac agacttaaat aaagagccag atgcatctga gggaattaag aacaaagaag
      721 atttaccaaa aggaactact tatgcttgga aagagaaagt agatgttagc acagctggaa
      781 acaaaaaagg tactattgta gtgacatatc cagatggatc atctgatgaa gttgaagtag
      841 acgttacagt gacagatact cgttccgatg cagataaata caatcctgaa ggacaaaaag
      901 tgacgacaga cttaaataaa gagccagatg catctgaggg aattaagaac aaagaagatt
      961 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
     1021 aaaaaggcac tgttgtagta acatatccag atggatctaa agaagaagta gaagttacta
     1081 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
     1141 accaaattgt tactggtaaa actgaaccaa atgcagaggt aacagttaca ttaccagacg
     1201 gaagtcaata ccatgggaca gctgataaga atggtaattt tacagttaaa gttcctaaat
     1261 tagaggcagg gacaaaagtg agagtaactg caacggatga atctggtaat actagtgaac
     1321 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
     1381 gtaagacgga caatcaagat ggcaatagca atcaagacaa aaatagaggc aaaagccagt
     1441 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
     1501 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
     1561 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
     1621 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt
//
LOCUS       DACEZN010000279         1670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.291, whole genome shotgun sequence.
ACCESSION   DACEZN010000279 DACEZN010000000
VERSION     DACEZN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.291"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            185..382
                     /locus_tag="IXH60_002861"
     CDS             185..382
                     /locus_tag="IXH60_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991734.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            488..970
                     /locus_tag="IXH60_002862"
     CDS             488..970
                     /locus_tag="IXH60_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991735.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1028..1624)
                     /locus_tag="IXH60_002863"
     CDS             complement(1028..1624)
                     /locus_tag="IXH60_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1991736.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          503 a          258 c          341 g          568 t
ORIGIN      
        1 gttttaattt tttgaaaaaa attcttctaa atacaacagc tgttttaatt gtgaaaacga
       61 aaacagtacg ctataatgcc tgtaaggttg atttttaact gtaaataaaa atcaatgagt
      121 taaatctcca aagaaatagc agtggtaatg tggagaaaag ttccagcagt ttaggaggat
      181 tttgatgacg gaagaaatga ttaatttagg agaacaatat gcatgcaaac caattggttt
      241 tacaaaaacg gtcattggtg aagttgtatc aaaaatgaca aactgcgccg tggtcaaagt
      301 tgcccaatgt gctgcagagg accaagaatt attagatgaa aaagcaagca tggttgttgc
      361 aaaatatgat acatttgaat aaagaaaaca attggacgtg cggcgtgctg caagtccaat
      421 ttctttgaga aggtaaatca gtattctttt cctattttct ggagaagaaa aagcgaggcg
      481 agttgtcatg gtaacattct ttacggttct attgttttta ttcgttgttg tgatgttttt
      541 atttctttgg ctggttcgaa aagaaattat ttatcggact gtgcgaaatc gatgggtcta
      601 cttggtgata cccttcttag tgtcactagt catttggtat acactcatca gtcatcctac
      661 agcagatgaa ttggcaaaag ggatcttgtc agcaatgatt tttattagtt ttttgttgga
      721 cagtagagga atcacagaag aaggacttgt tttgaatagc tttgacaaaa aaggtgttcc
      781 tttttctgag atcaataaaa ttgtattata tcagccaaaa ggttctaaaa tcgtaaaaat
      841 gaattttttc cgaaacggat ggcgaggacc gatgctgaaa ttttctgcat cattagaaga
      901 attagttcca tttttgtccc aacgcttaaa cgatgaggca gagattgata ttatgattga
      961 tccagaataa aaacagaggt tgcgacaaga tgtttgtcac aacctctgtt ttttatttca
     1021 aataaaatta tccaaatacg cggaagcgat cggcatcgtt catgtattct ttttccccag
     1081 ccggtgtttc tggtgaaccg aatactaatt gcgaacgtag tttccagttt gaagggatat
     1141 tccattcttt tgcaactgct tcgtcaataa ctgggttgta atgttgcaag tttgcgccca
     1201 atcctttatc aactaatgct acccaagtat tagctgttgc gattccgttt gattgttctg
     1261 accaatctgg gaagttgtct gcatataatt caaattgttc ttgtagacct tttaccacgt
     1321 cagtatcttt gaagaataat actgtaccat aaccattttt gaaaccagct aatttgtttt
     1381 gtgtatttgg gaaagcttct gctggagtca atgggcgaag tgcttcttca gtgatttccc
     1441 aaagtttttc atgtgcatct ccgaaaagga taactgcgcg tgttgattga gcattgaaag
     1501 cagttgggct ttctttaatt gcttctttga tcaaagttgt taattcttca ttagaaagtg
     1561 aaacattacg tcctagatga tagattgaac ggcggttttt caaagtgtca gtaaaagttg
     1621 tcataataaa atttctcctt tgatttgcgt tttgcatttt tttaattgat
//
LOCUS       DACEZN010000280         1653 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.293, whole genome shotgun sequence.
ACCESSION   DACEZN010000280 DACEZN010000000
VERSION     DACEZN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.293"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            155..>1653
                     /locus_tag="IXH60_002864"
     CDS             155..>1653
                     /locus_tag="IXH60_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1991737.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIA"
BASE COUNT          613 a          233 c          267 g          540 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgc
//
LOCUS       DACEZN010000281         1631 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.294, whole genome shotgun sequence.
ACCESSION   DACEZN010000281 DACEZN010000000
VERSION     DACEZN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.294"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            165..359
                     /locus_tag="IXH60_002865"
     CDS             165..359
                     /locus_tag="IXH60_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991738.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            628..927
                     /locus_tag="IXH60_002866"
     CDS             628..927
                     /locus_tag="IXH60_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991739.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1024..1458)
                     /locus_tag="IXH60_002867"
     CDS             complement(1024..1458)
                     /locus_tag="IXH60_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991740.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT          591 a          255 c          237 g          548 t
ORIGIN      
        1 gaacaatagc cttttattat atatattatt caaattagtt atatcgagtg aaaaaaactt
       61 tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt tcacttacat
      121 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
      181 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      241 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      301 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      361 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      421 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      481 atgattattt caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat
      541 caaatatact tgtcattcat catgaaatat agagacttat aaaattgttt atttttataa
      601 tgtatcagta tagaaaggaa ataaaacatg gattatactg ttgaatttga cagtgaacta
      661 cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc ccacaaagtg
      721 acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa
      781 ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc atttgcttta
      841 aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat tgtatttgat
      901 aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga gtgatactaa
      961 gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt acgttattat
     1021 atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga cataagaaat
     1081 accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt ttgttttctc
     1141 agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct tctccaaagc
     1201 ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa tgaggatatc
     1261 ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga taatcttccc
     1321 ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta accttgcagt
     1381 gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct catcgatcat
     1441 aagggaatca atattcattt ttttcttcct ttcttataca attctccctc taataataca
     1501 gttctttgac tatttaatct atcacatata actctcctgt aaagattctc tcgattcctt
     1561 aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt tgtgataaat
     1621 tttcttcaca a
//
LOCUS       DACEZN010000282         1610 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.295, whole genome shotgun sequence.
ACCESSION   DACEZN010000282 DACEZN010000000
VERSION     DACEZN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.295"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(578..1192)
                     /locus_tag="IXH60_002868"
     CDS             complement(578..1192)
                     /locus_tag="IXH60_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1991741.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          528 a          299 c          252 g          531 t
ORIGIN      
        1 ttccttaaaa ttcttttttt atttttagat tagagaaaat ctcgtcctgt ttttttcatt
       61 ttacaatcat tgaaaaattg aatttcattg caaaaaaaat aataaacact tataaatatc
      121 cattatctat ccttcaattt ttgtgtattt tatttctatt agaaaaaggt atcctgttgg
      181 acaattttgt tatatcaggt aaacttcatt gcaataatga taacaaatgt actataatta
      241 ctttgtaaag agttttgggg aagactcttt catcaacgtg tattaagaga caagaaagaa
      301 tccatatgga ttctttcttg tctctttttc cactactttt tatttttctg acactacata
      361 ttcttataac tcatttctca taactgtgaa acaacttata aaaataacaa actttaaaca
      421 aaacaagctt aaaatcaaag atgatccatc ttaaaataac taaaacaaaa aaagtagtcg
      481 ttgttaatcc tcattaataa tcgttttctt tttttcaaac gctccatttt tatgtggaac
      541 attatttttt taaggaactt tgtcaacatg tggataacta ttttccactt gtgtcctgga
      601 ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg
      661 ctgttccgca gatcgctgga tctagatttg gcaaagtcaa gactgcatca tgtaaattgg
      721 cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg caaaccactg ctatgttgcg
      781 atttgatact tcgtgacaaa tcaaatccag taacaatgat taatggatga ccattttctt
      841 tgatccactg agctagttcg atgattgccg ggtttcttcc ttcattcgaa agaagaagaa
      901 aaatgtcttc tgatttgaca gaaagctgat caataattgc ctgtcctgca ccttctagcg
      961 tttcatagac acctccggct ggattttcaa tcaatatact tggtacaaga tccggggttt
     1021 gatcccaaaa ttcaaatgct acaccactta gtgtgcggct agcatatatc tgcaatctac
     1081 caccttgatt aatttgtttc gcgatccaag tgccagcttg aagcatcagc ggctcttcta
     1141 tccttaattt ctcttccaat tggtgataat agtcaaaata acataaactc atttatctct
     1201 cccgcctagc tatacttgac atgaatagaa acagctgcct aatctaagga agtacataaa
     1261 aaaacagtag ttagacagta acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat
     1321 tgaacgaata cgcccaatac cttgacatag caaataaaac aacgtgcaga ccatggaaaa
     1381 tatctacata ggggtagaat tttcacccta gattaggtac aataaattag gtcgatgcac
     1441 tgcattgact atggcaaaat gatcggtcag ggtgtatgtg tgtgactggt cattccctga
     1501 ttcttagcgc caactaaggg tcagggagcc tttttttatt cagctgtttc tatctgaatt
     1561 tattgtaagt aatctcttat ccaagtgcaa gcaagaaaat acaaaaaaac
//
LOCUS       DACEZN010000283         1606 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.296, whole genome shotgun sequence.
ACCESSION   DACEZN010000283 DACEZN010000000
VERSION     DACEZN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.296"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>1606)
                     /gene="nifJ"
                     /locus_tag="IXH60_002869"
     CDS             complement(<1..>1606)
                     /gene="nifJ"
                     /locus_tag="IXH60_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1991742.1"
                     /translation="DGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSAAGKENVF
                     GQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGELLPSVFH
                     VASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASIEGEVPFL
                     NFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTNQNPDIHF
                     QQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMGSVAQTIE
                     QTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGAGGEPLLL
                     DVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFTIGIVDDV
                     TYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQGYFYYDS
                     KKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGGIFLLNTL
                     WSDEQLETHLPLKLKRYLAENNIRFYT"
BASE COUNT          441 a          360 c          309 g          496 t
ORIGIN      
        1 tcgtatagaa tctaatgtta ttttcagcca agtaacgttt taattttaag ggcagatgcg
       61 tttctaattg ttcgtcgctc caaagagtat tcaataaaaa aatgccacct ggcttcaatc
      121 ctttgaccaa gtcatagcta tgcaggtaag ctggtgtgtg acaggcaaca aggtccgcat
      181 gctcgacaag atacgctgaa cgtatcggcg tatctccaaa acgaagatga gagacagtca
      241 gaccgccaga ttttttcgaa tcataataaa aatagccttg cgcatattta tcggtgtgat
      301 ctccaataat cttgatagct gatttatttg ctccaactgt tccgtcagat ccaaatcccc
      361 aaaacttggc ctggaatgtt tgcggttctg tcaaatccag actttccttt ggttccaaag
      421 attgataagt cacatcatcc acgatcccta tggtaaaccg ttttttgcgt atgcttgggt
      481 cttttttcaa ttcatcaaaa actgctgaga tttggtcggg agtcacatct ttagacccca
      541 atccgtagcg accaccaata acagccgggc gaagttcaga atcatataac gcactttgaa
      601 cgtctaataa gagaggttct ccacctgccc ctggttcttt acttcgatca agaacagcaa
      661 ttgtttttac tgtatgaggc aacttttgca cgaatgtttc cactggaaaa ggacgataaa
      721 gatggatatt caaaaaacct gttttccgtc cgtttgcatt caagtaatca atcgtttgtt
      781 caatcgtctg tgcaactgat cccatagaaa caatgacttc ttccgcatcg tctgctccgt
      841 aataagtcac caagtcataa gaagttcctc gtaatcggtt gatctctttc atatattttt
      901 gaacgatttc aggtaattta tcataatagg ggttgatcgt ttcccgttgt tgaaaatgga
      961 tgtctggatt ctggttcgta ccactaacag tcggatgatt gggattcatc cctcgtttac
     1021 gaaatgaagc taatttctcc tgattgatca gaggagctaa ttcttcatat tccaaaactt
     1081 cgattttttg aatctcgtga ctcgtacgga atccatcaaa gaaattcaaa aaagggactt
     1141 ctccttcaat agaagcaaga tgggcaacag gtgctaaatc catcacttct tgtacactgc
     1201 tttcagctaa catagcaaat cctgtttgtc ttgcagccat cacgtctccg tgatctccga
     1261 aaatattcaa cgcatttgtc gtcactgccc gactagcaac gtggaaaaca cttggcagta
     1321 gttccccagc aatcttgtac atattcggta tcatcaaaag cagcccttgt gaagcggtat
     1381 atgtagttgt cagtgcacca gttttcaatg atccatggac cacaccagct gctcctgctt
     1441 cggattgcat ttctactact tttactggtt gaccgaatac gttttctttt cctgctgctg
     1501 accattgatc tactaattca gccatggtcg aactgggtgt tattggatat attgcagcaa
     1561 tctcggtaaa tgcataagaa atatatgcgg ctgccgtatt gccatc
//
LOCUS       DACEZN010000284         1600 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.297, whole genome shotgun sequence.
ACCESSION   DACEZN010000284 DACEZN010000000
VERSION     DACEZN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.297"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            183..470
                     /locus_tag="IXH60_002870"
                     /pseudo
     CDS             183..470
                     /locus_tag="IXH60_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            484..915
                     /locus_tag="IXH60_002871"
     CDS             484..915
                     /locus_tag="IXH60_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991743.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            917..1054
                     /locus_tag="IXH60_002872"
     CDS             917..1054
                     /locus_tag="IXH60_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1991744.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1091..1384
                     /locus_tag="IXH60_002873"
     CDS             1091..1384
                     /locus_tag="IXH60_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991745.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1381..>1600
                     /locus_tag="IXH60_002874"
     CDS             1381..>1600
                     /locus_tag="IXH60_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1991746.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT          584 a          233 c          343 g          440 t
ORIGIN      
        1 aagacggagt tgctaaatac ttaaaaacgc atgtagaagc tattgatggt aaagaagcat
       61 tggtgcagac agatggagac caagccattg caggacataa gaatttttca ggttctgtaa
      121 caatcaataa taaacgtgtt ttgacgactg atgataacag atatgaagtg gtaaatctgg
      181 ttgtcactaa cggtaataca gggacagcaa agctttatcg tgaaggaaaa acagtttcta
      241 tatattttgt ggctttaaac ggaaaaagta gtggcgggaa tgattcggtt attttaactg
      301 ttccagaagg ctatcggaca ccaattagtt ttgaacaact ggttgggtca atagaccgtt
      361 ctactttgaa cagtgctcaa ctatctattg gcgcagacgg agccattaaa tggagaagaa
      421 attcaagtta tggatcagct tattcatttg ttatcactta ttcaatttaa gggaggaaat
      481 ctaatgaaag tagtttacaa atcaatcaag ccttacggat tcgagcaaat cattttgaac
      541 aatcaagaaa atatccctga aaactgtaca gagattaaac caccagttcc taactggaga
      601 ccaagatttg attttgataa aaaacagtgg gttgagttag ctactgaaga agagaaaaaa
      661 gggaatgctg ttgatagcgt agatgaattg gctaatatta aggcattata cgaaacgctg
      721 aaggcagaaa atgatgaatt gaaacagcta aattctaaag caatgctaaa caatgtagca
      781 atcaagcaag aaaatgtctt attgaaagaa aagtcagaaa gtttagcgca gttgaattca
      841 aaaacgatgc ttgcttctgt acaaaatacc aaggaaatcg aagaaatcaa aaaacaatta
      901 caaggtggga agtaacatgt attcatatga agatatcaaa ctaatgtatg actggggctg
      961 tttcactaac gaacaagtaa tggttttcgt tccattatgc attacagacg aagaagcaga
     1021 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1081 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1141 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1201 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1261 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1321 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1381 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     1441 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     1501 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     1561 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca
//
LOCUS       DACEZN010000285         1569 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.298, whole genome shotgun sequence.
ACCESSION   DACEZN010000285 DACEZN010000000
VERSION     DACEZN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.298"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            188..940
                     /locus_tag="IXH60_002875"
     CDS             188..940
                     /locus_tag="IXH60_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1991747.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            930..>1569
                     /locus_tag="IXH60_002876"
     CDS             930..>1569
                     /locus_tag="IXH60_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAQ1991748.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQS"
BASE COUNT          444 a          302 c          293 g          530 t
ORIGIN      
        1 gagcttgatt tcattaagca gctttctaaa gaaactttcg gttacttgta cattgcagcc
       61 actcgtttat cgcaaaaaaa gtatgcaggt tattttttcc atagcaaaaa agagaacttt
      121 ttctttacct ctccctcgtt tacaaatcgg cttcccagca atctttgcag aaaaaaaaca
      181 agagattttg attcttacaa ataaaccgca agaagtccaa ttgctttggc agccaaaaga
      241 aagaggatgc catgaaacat taactgtcat ttttacagcc tatgatagtc taggactttt
      301 cagaaaaagt gcaaaaagag agatctcttt tcctgtgact gttctgcctg ctttttgtaa
      361 gattcaagca gaaaaattac agagagttct ggaaaaaaaa cagcagctga acgcgtacca
      421 aagaggtctt gattttcggg aacatcgcgc ttatcgtcca ggtgattctt tcaaccgaat
      481 cgattggaaa ttgtctgctc atactcaaga gtggttatac cgtgaatacg attatcaaga
      541 agaagaatgt tctcctcttt tttgcttttg gggttcgcac agtccaaaat ttgaacctct
      601 gctagacttg tatttttctt tatggcattt gagaaaagaa aagcagccgg aacttctgat
      661 attaggcaac cgcgcctttc atggcaaaga ccccggtcat acctattttg cttctttgga
      721 agctggcgat gagaaagctt ttttcgcaca cctaaaaaaa tatgcaaaaa aacaaatcgt
      781 cctctttgtt cctgagaatt ctcctgaagt ctcagaagct attgaaacat tgtcaaaaga
      841 tcgaacggtt tatcttgttt atttcgtaga aaaacaactc atggtccaag aaaaagacaa
      901 gatatgttca ttgccaggag gtgaatggat tgacgattaa atgggaaaaa tggctgtgtg
      961 gcgtttactc ctttgtgata ctaagtgtag gtgctccgat atttgtagta gctaatcaat
     1021 tgcaaaacag ctaccttctt atcctaacta ttggtgtcct atgccttatt gtctgctgta
     1081 ttcctaaaaa atgggtacgt ttgctgcttt ctttgccagt aatgataggt tgtttgtttt
     1141 tgtattttcc ttcgaagaca ttttccttat tatggatctt acagtttttt tcgtcagtca
     1201 cagaggaatt cttccatcta tcccaaggta aactttatta tttgccagaa aagacagctt
     1261 tttttctcat aatgttgctg atctatttat ttattacatt gaccgtggat tacgactatt
     1321 tgtacgcacc ctttctttct ttgatggctt acttcatgat gcttaccgtt tttcgggaaa
     1381 aagatttact gtacgaaatg attgcggtag taactgtttt gttcgtgtat ctggcagcaa
     1441 ggcaattttt ctccatgcgg ttgttgtcgg gaaacactcg attccagtca ttgactacag
     1501 ttctgcttat ttgtttctta actattagcg ctatcttgcc tttatatctt ccgaaagtac
     1561 accaatctc
//
LOCUS       DACEZN010000286         1557 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.299, whole genome shotgun sequence.
ACCESSION   DACEZN010000286 DACEZN010000000
VERSION     DACEZN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.299"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(62..280)
                     /locus_tag="IXH60_002877"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IXH60_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IXH60_002878"
     CDS             complement(546..740)
                     /locus_tag="IXH60_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991749.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          548 a          237 c          209 g          563 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaac
//
LOCUS       DACEZN010000287         1497 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.302, whole genome shotgun sequence.
ACCESSION   DACEZN010000287 DACEZN010000000
VERSION     DACEZN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.302"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            100..750
                     /locus_tag="IXH60_002879"
     CDS             100..750
                     /locus_tag="IXH60_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991750.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            937..>1497
                     /locus_tag="IXH60_002880"
     CDS             937..>1497
                     /locus_tag="IXH60_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1991751.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          485 a          178 c          296 g          538 t
ORIGIN      
        1 tgataatcat aagttcataa atagtaattg ggatgtatgg ttggtatacg ttatacctga
       61 acttgaacta tcaagatttg gtttagaatt tgagaatcga tgtttataaa gggagggcaa
      121 aaaatgtgct tttttacaac ttttactatc attatgattg cagtactttt tatttttcta
      181 gatatagcaa agcgaaatac agcattttta ttatatcggg ttttacttcg agcaggtctc
      241 ataacattta tatcgatcgt tggatttttt ctattcacag ttattgtatt tatttggaga
      301 actacagcac caccattgcc agagattaca tatggagaat ttccgtttag tcttgaatat
      361 gaaatagatg aggaaagaat tttgattgaa gatacggtga ttgcagaatt tataggaagt
      421 tttagaggga atgtgacaac tcaacctaga cgaagttgga gtataaaact tttaaataat
      481 agtggagagt tgaaagcatt tcattatgaa ttcttaataa tagaaagtga agatgtaaca
      541 atcactttaa gtagtggctc tgctggatat tttatgaatg atggtggaag caagccacag
      601 ctcacagtcc gtgatcgtat tgcaggtttt atgcattttt ctcactatga cttagaagaa
      661 gctcgtaaaa tacttgcaga atatgggatc acattaatca attgggaata tgcgccaccg
      721 ataagaaatt ttttcaatga ttattattag tcttataaaa aacagaatgt aaaatttatg
      781 tgcgcgagta tttagttttc cattttttgt ttgatagata tatagcattg ttgaagcatg
      841 ggatagtggt gccaatttta tcaatgaggt tggtcaggca gttaacataa ttggtgatgc
      901 tataaatcca tttaattgga attggaggaa ttaggtgtgg aaacagtgag attatggata
      961 ttcatgaaaa attttcgttc ggctttgaga tatgttattc tggaatcaaa atcaggttat
     1021 tatctgattg acacagatca ctatactttt tggggaggct acctttttcc gatttttaat
     1081 tggataatcc cccataaggt tatgaaaatt aacttgcctg cagaagaaat aaatagatta
     1141 atactcaaca aagatttaca aaggaaaaga gagaaagaac attctaatgt tgcttggata
     1201 ggtccagcat ttgcagtagt gacttcttca attgtcggtc ctatgctaga gagtatttat
     1261 agtgattttt ctattggaat acgttattta tttgttactt taagtatagc tgttcttttg
     1321 tgcataaagg gttatgttag caagaagaat aaagatattt taacactaat cggtgtaaac
     1381 aatttatcta gtactaaaat tatactgttt cctgaatcta taaaagaagg atcaaggttg
     1441 ttgataattt ttataatgct tatactgttt tctgttttat ttggggtgat gtttata
//
LOCUS       DACEZN010000288         1445 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.303, whole genome shotgun sequence.
ACCESSION   DACEZN010000288 DACEZN010000000
VERSION     DACEZN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.303"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            67..222
                     /locus_tag="IXH60_002881"
                     /pseudo
     CDS             67..222
                     /locus_tag="IXH60_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            241..>1445
                     /locus_tag="IXH60_002882"
     CDS             241..>1445
                     /locus_tag="IXH60_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1991752.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIV"
BASE COUNT          587 a          127 c          244 g          487 t
ORIGIN      
        1 tccagtgtgt tttttattat tctacacacc ttattttttc tgtctagtct agtgtagccg
       61 attcaggata aggataagtg tattaattgt ggaaaatgta aaaaagtttg ccaaatgaat
      121 aaagattcag ttgagaattg caatcatctg gagtgtatca gatgtggacg atgtaaaaat
      181 gcttgtccag tggatgcaat tagttatgga gtaagaaaat agagaggagt agttaggaaa
      241 gtgataaaaa atgctatagc atatattaca agaaagaaaa atagaacttt aataattttt
      301 atcatattaa ctatagttct ttcttgcttg tattcttgtt taacaataat gaaatcaagc
      361 aatgaaatag aaaaggcttt atatgaaagt tctaattctt caatatcaat aacaaaaaaa
      421 gatggtaaat attttaatgt taatcaattt aaagatattg aaaaattaaa agaaattgaa
      481 gaaaaaataa ttcaatatga tggattagca aaattaaaag atgctaaagt agttagcgga
      541 gaacaaagaa taaatagaga agatttatct gacgaattta agaatgttgt ttcactcgaa
      601 gctacaaata atactaaaag aaatatttta tttagtagtg gagtatttac aattaaagaa
      661 ggtaaaaata tagaagaaaa tgataagaat tcaattattg ttcatgaaga atttgctaaa
      721 caaaacaatc taaaattagg tgatgaagtt gatcttgaat tactagatat tgaaaaaagt
      781 ggaaaaataa aaagtcataa atttaaaatt atagggatct tttctggtaa aaaacaggaa
      841 acatatacag gattatcttc tgattttagt gaaaatatgg tgtttgtaga ttattcaaca
      901 agccaagaga tattaaataa ttcagaaaat aatgaaattg caaataaaat tttaatgtat
      961 tctggtagtg cagaatctac agacttagcc ttaaacaaat tgaaagagct taaaattgat
     1021 gagtcaaagt attttgttga aaaagattct aatgcgtttg aagagtcttt agagtcagtg
     1081 agtggaataa aatatataat aaaagtaatg acttattcta ttatgttagg tgggatggtt
     1141 gttctttcat taatcttgat tctatggtta agggaaagaa tttatgaaat aggtatattc
     1201 ttatctattg gaacatctaa gatacaaatt ataatgcaat ttatattcga gttaatattc
     1261 atatcgatac caagtataat atcttcctta tttttaggaa atgtattact aaaagtaatt
     1321 gtagatggat tcattaactc agaggattca atgatttccg gtggaagttt aataaataat
     1381 agtagtttta tgtcaaatat aacaacactt ggacaaagtt atttaatatt aataagtatt
     1441 attgt
//
LOCUS       DACEZN010000289         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.304, whole genome shotgun sequence.
ACCESSION   DACEZN010000289 DACEZN010000000
VERSION     DACEZN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.304"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(150..1337)
                     /locus_tag="IXH60_002883"
     CDS             complement(150..1337)
                     /locus_tag="IXH60_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1991753.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEZN010000290         1413 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.305, whole genome shotgun sequence.
ACCESSION   DACEZN010000290 DACEZN010000000
VERSION     DACEZN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.305"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            67..1368
                     /locus_tag="IXH60_002884"
     CDS             67..1368
                     /locus_tag="IXH60_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1991754.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          464 a          286 c          257 g          406 t
ORIGIN      
        1 aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat caaaggagcg
       61 ttctgtatgc ctgatcttca tcatatcaaa ctactacttg gaatcaaaga taaaaacatt
      121 ttcatcacaa atgtggaatc gaagagcatt aagaatatca agagtcttgt tgtttctgcg
      181 acgttaagta aagaaattcg tcgctgtcct ttgtgtaaac agatgaatca tgaaggcatg
      241 atcgttaaga acggaaagaa aaaatcgctc attcaactta ataaatgtgc gaaccaactg
      301 acttatcttg ctttagcgaa acaacgctac cactgtcgtg gctgccacac ctattttacg
      361 gcgaatacct acattgtcga tagaaactgc tttatcgcta aacaagtgcg ctataaaatt
      421 ttagaagagc taactgaaaa acaagcaatg acgacaattg ctaagcattg cggcgtttct
      481 tggtcgaccg tttctcggac actcgcttct ctgatcccca tgacaaaagt aaaaagaaat
      541 tggttaccgc gttgtttatt aatggatgaa tttcgttcac tgaaaaatca agtcggtccc
      601 tactcctttt cttgtatgga tggcgatact ggaaaattac tggatattct tccttcacgg
      661 aagaaaaagg ctctcgtttc ttattttatg caatttgaac gacgcgcacg attgaaggta
      721 aaggtcatcg tgacggacat gaatgtttct tatgcctctc tgataaagga atgttttcca
      781 aaggcaaaac tcgttgtgga tcgttttcac attgtcaaac atctcattcg taaatttgag
      841 gatattcgcg tacgaatcat gaaaagcttt gatcgaaacg atccaacaca agccaaatat
      901 tatcgacaat taaaggcgtt gtctcggctt ctgatcaaac gacaggacaa gcttgtttat
      961 gacaaatgga ccaaatggcg caattttggt tgggcgtatc tcaccgaaag tgaagtggtg
     1021 gatcgtctgc tttcgatctc tgacgaatta cgaatcgcct atttttacta tcaagaaatt
     1081 cttcaagcgt ttcacgacaa agaggcggac actttcttta aacttgtaag aacgatgccc
     1141 aattccgttc caaaagaact gcattctata aaaaaagctt ttataaacta tgaatccggc
     1201 attcgtttag ccctcgaatt gccttattca aatgccaaaa tagaaaatct gcatacccac
     1261 atcaaagcat tgaaacgagt cgcttatggc tttcgaagct ttcggaaaat gaagaccaga
     1321 attttcttac taaataatct gattacctat gaatcaaaaa acatctaaag tagaaaagga
     1381 attctacctt agatgttctt atctcactct atc
//
LOCUS       DACEZN010000291         1377 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.307, whole genome shotgun sequence.
ACCESSION   DACEZN010000291 DACEZN010000000
VERSION     DACEZN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.307"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            56..1351
                     /locus_tag="IXH60_002885"
     CDS             56..1351
                     /locus_tag="IXH60_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1991755.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          432 a          274 c          262 g          409 t
ORIGIN      
        1 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
       61 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
      121 aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct
      181 tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg
      241 ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct
      301 ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac
      361 ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg
      421 cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc
      481 ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc
      541 aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt
      601 tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc
      661 ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat
      721 tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca
      781 gttgatcatc gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg
      841 agtgaaagaa accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa
      901 aaaattgaag cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa
      961 acgggtttgg cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact
     1021 gttagctgct tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc
     1081 ggtaaagaaa agagatcagt cactatttga ggattgttta actagagaca ttagcggtct
     1141 gccagaaacc tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa
     1201 tgcgcttcac tacagctatt caaacggacc attggaatgc ttaaacaacc acatcaaagt
     1261 attaaaacgc aatgcgtacg gctttcgcaa cttttacaat ttcaaactac gaattatgat
     1321 tcgtcatggc aaaacatttt taaccaaata aacagaacag aaagaggttt tcttctt
//
LOCUS       DACEZN010000292         1355 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.308, whole genome shotgun sequence.
ACCESSION   DACEZN010000292 DACEZN010000000
VERSION     DACEZN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.308"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..196
                     /locus_tag="IXH60_002886"
     CDS             <1..196
                     /locus_tag="IXH60_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1991756.1"
                     /translation="KLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPSGS
                     LDSHVQLVDLRPEKFSGIKQ"
     gene            complement(310..939)
                     /locus_tag="IXH60_002887"
     CDS             complement(310..939)
                     /locus_tag="IXH60_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1991757.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1073..1145)
                     /locus_tag="IXH60_002888"
     tRNA            complement(1073..1145)
                     /locus_tag="IXH60_002888"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1110..1112),aa:Thr,seq:ggt)
BASE COUNT          474 a          207 c          224 g          450 t
ORIGIN      
        1 taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt caaaaatgta
       61 catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta ctcttgaaag
      121 cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag aaaaattctc
      181 tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat caagctgtat
      241 tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat tttgtcgtct
      301 ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta tttctggctt
      361 ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat tcgttgaaag
      421 tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc ataaaatcaa
      481 tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa gtgaacgaag
      541 tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag aaggtacgca
      601 gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa attgtggagc
      661 gactaaaaca acctttttta aatatggaga aggagttgca agatagttta aagcaataac
      721 agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca attgtacttc
      781 gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat gattgaacaa
      841 tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt tccatgattg
      901 ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct ttttatagtt
      961 atcatataga ctttctttgt actattaaaa tataaacatt catttcttac gtcataaaat
     1021 aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag gaagccactt
     1081 gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag ctgagctaaa
     1141 gcggcattat cattaataca aataatagga taacgaatat taatgtattt gtcaacagaa
     1201 ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa ataaaaataa
     1261 caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa agtattgcgt
     1321 tatttagaag aaattgatat tatattcatg tactt
//
LOCUS       DACEZN010000293         1342 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.309, whole genome shotgun sequence.
ACCESSION   DACEZN010000293 DACEZN010000000
VERSION     DACEZN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.309"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            82..>1342
                     /locus_tag="IXH60_002889"
     CDS             82..>1342
                     /locus_tag="IXH60_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ1991758.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIR"
BASE COUNT          456 a          252 c          258 g          376 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aa
//
LOCUS       DACEZN010000294         1340 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.310, whole genome shotgun sequence.
ACCESSION   DACEZN010000294 DACEZN010000000
VERSION     DACEZN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.310"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(60..>1340)
                     /locus_tag="IXH60_002890"
     CDS             complement(60..>1340)
                     /locus_tag="IXH60_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1991759.1"
                     /translation="ISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEIVDSKVTI
                     EKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYFGVFNG
                     FSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPIGKRFF
                     SDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAFGLAEA
                     HFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFDFEMTS
                     FTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVGICPVS
                     YFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSDCLLEKFNK
                     EQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          439 a          241 c          195 g          465 t
ORIGIN      
        1 tttgaaaaaa acataaatca gctaataaat atgtgagaca aagctgccat ccataaatac
       61 tagctgattt tttcagttaa atgtctattt ttttctctga tgatggtatt tccaaaatat
      121 tcccagtcat ctggagtcgg tggatttttc caaattatct gttctttatt aaatttttca
      181 agtaaacaat cagataaata aatattcgta cgagaagtga ttcgactctc aatcacaata
      241 ttgtaatttt taaatccttt cacttgagaa ataatgtaat cggtataagc agatccttgc
      301 gaaaaatcaa tacagacatg aatcggtgct tcaaaatatg ataccggaca aataccgacc
      361 aataaaaaga gataatcata aaataagcga atcatgaaac gttcatcttt tcccaagaaa
      421 tagcgtttat tctcttgtat gaccttttgg attgctgtac tgaatattgg atacgtctcg
      481 aagaaaaact gtagctgctt tttagacgta aagctagtca tctcaaagtc gaatatccaa
      541 ttttttcgat tgacatgcat catacccata cgaagttctt ttcgtatata tagtctttct
      601 tcttcagaga tttcaatcga aaatggtaat tgatcaatca attccactga cattttttga
      661 ctaatcatgt ggatatcatg aaaatgagct tccgcaagtc caaatgcgcc atctaattca
      721 aattcttctt ccgtgatcaa aaacaacatg atataagaaa tctcatcaat gatctgctct
      781 ttagggatat gaagaaaaga aatatgtgat aaatccttga tgatcttcat tccttctgag
      841 ctcttaaagt attcatctga aaagaaacgt tttccgattg gttgattccc ataggccctc
      901 aacaaactaa tagtggttag caaattgaat ttgattcgtt ttgttgcaga cagttttaaa
      961 ttgaacagat attcaaaata ttgcacgata ctctttgctt cattcatgac gctttctcta
     1021 aatggcatag aaaaaccatt aaagaccccg aaatataccc caaataatac gttccgtatg
     1081 tttatttctt ccccaactaa tatgtttttc tttaacttta tttgatgctg ttcaagaaaa
     1141 gagactaatt ctcttctttt tacataggct cttgatatgc ttaaaaaatg attctctgca
     1201 aatttttcga tcgttacttt tgaatccacg atctccgtta acagtcgagc aatttttgat
     1261 cgttgaaagt agattcttct taaatttttg atatccgtca aattcagacg ttcgacatgg
     1321 atctctgctt cttctgaaat
//
LOCUS       DACEZN010000295         1317 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.311, whole genome shotgun sequence.
ACCESSION   DACEZN010000295 DACEZN010000000
VERSION     DACEZN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.311"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            8..>1317
                     /locus_tag="IXH60_002891"
     CDS             8..>1317
                     /locus_tag="IXH60_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1991760.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKL"
BASE COUNT          452 a          243 c          272 g          350 t
ORIGIN      
        1 gaaaagcatg tctttacaat ttattcttgg aaacgggaaa aaagatcacc gtcaagtctt
       61 gatcgacgaa gcatccgctt ggctcgacca ggatgcaagt aaccaagtat ttttccttgt
      121 tcccaactac agcaaattcg aacaggaaca agaaatttta gcggagatgc gaagaagaag
      181 cggaaaaaaa actttcagca caacaaacgt tcaagtattt agtttttatc gtctggcttg
      241 gtatttgctg caacagacaa ctcttcttac aggaaatgaa ttatcagaat caggatctgc
      301 aatgatcttg cgacagattt tagaaaaaaa tgaggaagaa ttaacaatct ttcgcggaga
      361 aatcaataaa agagggttta ttcgtcaatt acaagaactc tacagtgaat tgcgtacagg
      421 aaatattcaa ccagaagacc tgatgctttt atttggtgat tcacctaatg cagaagacca
      481 gcaacttaaa atgaaagact tgaagctgat tttttcagct tatgacagcg aattgactca
      541 acgtgcacta acaaatgaag atgcactatc tatactagca aattatatgc agacacaaga
      601 tttcaacaac gtcaaattta ttgtcagtgg tttttctcga atcaatggtc aagagtatca
      661 attactgcag gcaatgatgg aaaaggggca attggttgtc gatttattgc tggatcgtcc
      721 ttatacagcg gatcttccag aagtacttac gctctttcat gagactggaa agctttattt
      781 ccgtcttttt caaagtgcaa gagaacggaa agtccctata ttagtggacc gatttgcacc
      841 agagcagact cgtccgacag agcttcaact gctgcaaaca atgtgggaag agacatctag
      901 ccaaaagaaa gcggcagtac acaaaatagg gggacaagag catctccatt tgtgggaagc
      961 tgaaaatcca actgaagaag tccgacaatt agctgttgaa atcagacgac tagtcagtga
     1021 agaaaactac cgttatcgtg atatccaagt gctgacaaaa aatatgtctt tatatggaaa
     1081 tatccttcgg ccgatctttc aagaaatggc aattcctttt tatatcgatg aagaacaaat
     1141 gatggaaaat catccgctga tcgaatggat caacagtctg ttcgctcttg accgctattc
     1201 ttatcggcta agtgatatta tgcgtttttt caaaactgaa ttatttctgt cgacggaagc
     1261 agaaaccgaa cttgatgaat ggaaacaaga gcgagatgaa ttcaggaaaa aactgga
//
LOCUS       DACEZN010000296         1313 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.312, whole genome shotgun sequence.
ACCESSION   DACEZN010000296 DACEZN010000000
VERSION     DACEZN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.312"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            96..>1313
                     /locus_tag="IXH60_002892"
     CDS             96..>1313
                     /locus_tag="IXH60_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1991761.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT          429 a          270 c          239 g          375 t
ORIGIN      
        1 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
       61 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
      121 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
      181 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
      241 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
      301 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
      361 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
      421 gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
      481 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
      541 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
      601 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
      661 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
      721 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
      781 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
      841 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
      901 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
      961 aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg cgacctggct
     1021 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg agccctgctt
     1081 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     1141 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     1201 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     1261 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gca
//
LOCUS       DACEZN010000297         1306 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.313, whole genome shotgun sequence.
ACCESSION   DACEZN010000297 DACEZN010000000
VERSION     DACEZN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.313"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(226..711)
                     /locus_tag="IXH60_002893"
     CDS             complement(226..711)
                     /locus_tag="IXH60_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HAQ1991762.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(713..>1306)
                     /locus_tag="IXH60_002894"
     CDS             complement(713..>1306)
                     /locus_tag="IXH60_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991763.1"
                     /translation="KYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIV
                     SGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGIS
                     IFVFGNKLESGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYF
                     DDSLKSKFQALEKLDTEGEVDKKVEQIVSLINSIQED"
BASE COUNT          416 a          259 c          145 g          486 t
ORIGIN      
        1 caagttgcgt ggcgtgcttt tgctagaaaa tatataactg ttctttataa agaaatataa
       61 ggattaaaca ttttttatca tagagtttta cttatctata gaattatgtc tgttcctaat
      121 tcagtttgat gaattaactg ataaaaaaat atctactatt taaagataca gggataagta
      181 agtaaaaaaa taacatgtca ttagatagta gacagttcca actctttatt tacctttttt
      241 gtatgctgca ctgtcaatcc aagttcctaa attgttgtat ccatcatcac ttatataatc
      301 atattggcca tcaataattt cgtcaaacca caaatattct ccatctggtt tttgcccaat
      361 tattttaatt actgattgac tagtagttgt catctggtta aaatctttta ttttacctat
      421 attaacggcc aaaatagcat ttcctcgctt ataactctcc tggatttcat atttaacata
      481 ttctcgatca agagtttctt tacctatcag caccacagtc actgatgttc caattaattg
      541 attatctatc cagtttttta tagcaatatc tccattacgt ttaactttct caaattcagc
      601 cttatctaca aaaccagctt cctttgttcc tcgcaaagtc cagctgtttc taactaccat
      661 tgctcttgtt acatctgcat aatggaaact aaaaaaaacc cttctcacca tattaatcct
      721 cctgaatact atttattaaa ctgacaattt gttcaacttt tttatctact tctccttcag
      781 tatctaattt ttctagcgct tgaaatttac tttttaagga atcatcaaaa taatcactga
      841 aattctcttg aatcttatca aacaaaacct tacttgcacc tttggtaaat ccaatcggaa
      901 ctactatatt ttcattttga acagataatt caaattcttc aatcattcca tctgctgtaa
      961 ctatttcacc tgattcaagt ttgtttccaa aaacaaaaat actaatccca gactctgcaa
     1021 taatgttttc tcgatatctt ctccactcat gaggcatatc aaggggaagt ggtcttaaag
     1081 aaagtacgtc ttgtatgtta ttttttctta aatttcttct ctgctttaga actcctgaaa
     1141 caatactatt accaattcca agtccgtaac ctgatactat tctataaccg gattcagaaa
     1201 gcctatatgc taatttctcg acaaatttta atcctttcga ttcagatgta aatggagtat
     1261 aatcaacagc acttcccgaa ataaaaatag tttttctcaa atactt
//
LOCUS       DACEZN010000298         1293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.314, whole genome shotgun sequence.
ACCESSION   DACEZN010000298 DACEZN010000000
VERSION     DACEZN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.314"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..702
                     /locus_tag="IXH60_002895"
     CDS             <1..702
                     /locus_tag="IXH60_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1991764.1"
                     /translation="FKHLETLNVIVEKVDYIFLEKPAFSESIDLTQYNYDYKNIYVAA
                     PLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAFKSMGGGVELDC
                     IHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYFGRVPT
                     RKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWN
                     DLYHANEVLKIAEEK"
     gene            703..>1293
                     /locus_tag="IXH60_002896"
     CDS             703..>1293
                     /locus_tag="IXH60_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ1991765.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSN"
BASE COUNT          495 a          147 c          219 g          432 t
ORIGIN      
        1 tttaagcatc tggaaacact aaatgtaatt gttgaaaaag ttgattacat atttttagaa
       61 aaacctgctt tttctgagag tattgatctt actcaatata attatgatta caaaaatatt
      121 tatgttgctg ctccactgag gtataaaaat atcatggaag ttgttcgtaa tagtattcaa
      181 agtaaaaaag tatattctgt aagagctatt tgttctactt atcttcctga ttggagaaaa
      241 ggtactgatt atagaaataa ttattctgcc tttaaatcta tggggggagg agtagaatta
      301 gactgtatac atgaattgga ttatattata aacctatttg gtttccctaa atcagtaaaa
      361 aaacagtttg gtaaatattc gaatcttgaa attaacagta atgatttagc aacatatata
      421 ttgagatacg atgataaaat tatagaaatt catttagatt attttggaag agttcctaca
      481 agaaaatttc aattgtttac tttagatgaa tgtatcaatg ttgacttatt aaataataaa
      541 ataagttttg aagaaaacat tgttaaagaa atgcctgaat ctgctaatga tatgtatgag
      601 aaggagttga aatatttctt tgaaaacgta gtaacaggca aagaaaactg gaatgattta
      661 tatcatgcaa acgaagtatt gaaaatagct gaggagaaat aaatggaaat tttatttact
      721 atttgtggta gagctgggtc taaggggttt aaaaataaaa atttgaaata tttggcagga
      781 aagcccctag tccatttcgc tttagcaatg atagatatct atcaacaaaa acatcctgaa
      841 cataattgca cgacttgttt gaatacggac agtaatgagt tagtagaatt gacgactaag
      901 ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat tagctacaga tactgtaggg
      961 aaaattgaag tgatacgtga ttcattctta caactagaaa gagaagataa tatatttgat
     1021 ttaattatag atttagattt gacttcacct ttacgtgaag tttctaatct tgaagaatta
     1081 atagatgaac atctaaaaaa tttagataaa gacgtctact tttcagttgt ttctgcaaga
     1141 cgtaatccct attttaatat ggtaaaaaga attgaaaatg aagttactat agttaataaa
     1201 tcaagataca catctaggca acagacacct gaagtttttg aaatgaatgc ctcaatgtat
     1261 tcttttaaac ctgaatttct aaaaaaaagt aat
//
LOCUS       DACEZN010000299         1202 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.316, whole genome shotgun sequence.
ACCESSION   DACEZN010000299 DACEZN010000000
VERSION     DACEZN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.316"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..969
                     /locus_tag="IXH60_002897"
     CDS             <1..969
                     /locus_tag="IXH60_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1991766.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          403 a          217 c          242 g          340 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tc
//
LOCUS       DACEZN010000300         1192 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.317, whole genome shotgun sequence.
ACCESSION   DACEZN010000300 DACEZN010000000
VERSION     DACEZN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.317"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            82..>1192
                     /locus_tag="IXH60_002898"
     CDS             82..>1192
                     /locus_tag="IXH60_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1991767.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREK"
BASE COUNT          395 a          230 c          232 g          335 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gc
//
LOCUS       DACEZN010000301         1182 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.318, whole genome shotgun sequence.
ACCESSION   DACEZN010000301 DACEZN010000000
VERSION     DACEZN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.318"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..119)
                     /locus_tag="IXH60_002899"
     CDS             complement(<1..119)
                     /locus_tag="IXH60_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1991768.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVV"
     gene            569..>1182
                     /locus_tag="IXH60_002900"
     CDS             569..>1182
                     /locus_tag="IXH60_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1991769.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT          423 a          207 c          198 g          354 t
ORIGIN      
        1 acaacaagtt ctgttgttaa tggatctaaa tttctttcta tacattcgac acttgcgtaa
       61 aagcttttca tatcgattaa aaagtaatca tttactggct cttttgaata atccatcata
      121 aataacactc cattctttca cattatacaa acatatgttc gatattgtaa agaagaaata
      181 gctaaataca taaaaaaaga agcataggta tgctatcacc ctgcttcttc ttttgatttg
      241 tatgatcaat ttattctatc actttactaa ttaatttgaa aatctaataa aaactctcac
      301 tctaaaagag tgagagactg caaattagag tacggacttt tttggagttc cgttctttaa
      361 tattaacaaa tgaataaaat ccttacaaca tttgagaata agttatcagt agacacttac
      421 aaaaaattct catactattt cctccatcat tagtttttgt ctatataagg tataccctaa
      481 ctagacacga ccgcgacatt gaaaaatctg gttttctcgt gtatgattag ggtgtttttt
      541 aaatagttgc agaaagtagg taaaaatttt gagagttgca tacatacgtg tctcatccat
      601 cgatcaaaat gaacaacggc agattgaaga aatgaaaaaa ttcggtgcag agagaatttt
      661 cattgagaaa caatctggcg caacgattac tcatcgacct gtttttcaag aagcactgga
      721 tttcgtccga gatcaagata tttttattgt ggaagctatt gatcgacttg gaagaaatta
      781 cgacgagatc atcgcttcag tcaattactt gaagaagaaa aatgtgcagc tgatcatcac
      841 aagtctaccg atcatggccg aagcaattgg caatcctctc ctggataaat tcattaaaga
      901 tttgatcatt caaattttgg ccatgatcgc tgaacaagaa cgtaccgaat ctaaacgacg
      961 tcaggcacaa ggcattaaga ttgcaaaagc gaacggtgtt tataaaggac gtcctaagct
     1021 atacagtgcc aatgcaaaag atccccaacg tcgtcttgtt tataaaaaca tcgttgagga
     1081 tctaaaaaaa ggtgttgcga tcgctaagat tgcaaaagac tacaatgtta cgagacaaac
     1141 cgtatataga ataaaaaaag acagcatggt caatcacgaa ta
//
LOCUS       DACEZN010000302         1174 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.319, whole genome shotgun sequence.
ACCESSION   DACEZN010000302 DACEZN010000000
VERSION     DACEZN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.319"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            63..1111
                     /locus_tag="IXH60_002901"
                     /pseudo
     CDS             63..1111
                     /locus_tag="IXH60_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          489 a          123 c          234 g          328 t
ORIGIN      
        1 ataccatagt ttggaaaaat aaaaaattat atttcatcaa cgtttcataa aaggtgtgta
       61 gaatgttatt gaaagtagct ataaaatgct attgtgtgaa aaataaagga ggaataattt
      121 atgaaaaaat agtaccaaga ggagcatgcc tattacttgc attatgtttg agcttaagcg
      181 cagttgggtg tcaaaaaagt aatgaaaaca ataaaataaa agaaggacaa tccataagtt
      241 ctgaagagga aggcatgtca attaatgcaa atggcaaaaa tgaaactttt aagccatcag
      301 attatacatt ggaggcaaag aaagaatatg tttatgaata tttagggctt aaatttaaac
      361 tctcagacaa atttagaaat tatatagctg ataaaaagat agcgatgtta gatgatcaaa
      421 gtcctataga taaagaacta aagtatgcta ttttgacatt tgagaaaatg acagaagaac
      481 aaaagaatgc agttatagaa aagatgggcg atggatataa aaattggcaa aatgagcttg
      541 aaagaattgg gactattggt atatttgaaa aaaatacatc agaagaaaaa aatctaaaat
      601 tataaaatgt gatactcata ctaaaatagg agtttcaagt gatggaaaat acgattgcta
      661 tttaagcaca aatagcggag ctgaaagtaa tcttcttgat gaattaaaaa gaactgagat
      721 tcaaattata gataaaaaag aacgtcctaa aaatggtttt gtgctatcag aaaaaactga
      781 tttagaaaat acagaagcat ttaacaaaga atcggttaaa gatttacgta aactaccaac
      841 aaaggacata aatggcaagg attttacaag taaagatttt gagaagtatg atcttactat
      901 ggtaaatgta tttgcaactt ggtgtacagc ttgtgtaaag gaaattcctg atttggttga
      961 ggttcagaat gaaatgaaaa gcaagggtgt aaatatcgtt ggagttgtta cagatcctgt
     1021 tgatgataac ggtgaaaaca aggaagcaat tgaaaagtca aagttaatac atgaaaagac
     1081 aaaggcttca tatcctttct taatgccaga tggaatggca acactttttt tgacaaaaat
     1141 tataaggtgt tgtgaatgct gcggtgttat ggct
//
LOCUS       DACEZN010000303         1137 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.320, whole genome shotgun sequence.
ACCESSION   DACEZN010000303 DACEZN010000000
VERSION     DACEZN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.320"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            75..692
                     /locus_tag="IXH60_002902"
     CDS             75..692
                     /locus_tag="IXH60_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991770.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            696..1019
                     /locus_tag="IXH60_002903"
                     /pseudo
     CDS             696..1019
                     /locus_tag="IXH60_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          413 a          167 c          253 g          304 t
ORIGIN      
        1 ggagctttga ttattgcaac tgttgatgat ccaaaacaga atgttttcac tcttgagaaa
       61 gttggggtga tttgatggtt aaagtaaaga gaatgatgga gactgatgaa tctggtgtta
      121 aacgtcaatt tatgccaatt acacatgttt ctgcagtttt aggcttagaa aaaatgatat
      181 caggacaatc aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt ttaacgaaag
      241 ctgatttagg attagagaat gcaattactg aactacctta tgcaagcgag acaagcgatg
      301 gtattcttac tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga acatatattt
      361 tgccgattgc tactcctgaa caattaggag gtataaaagt tggggaactc ttagaagtta
      421 ctgaagaagg tgttttatct gcaactaagc aaacagactt caatttctct gaagaattaa
      481 agggaaaatt agaatctcta aaaatattaa aagcaggtgc aaatatatca attgcagaag
      541 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgttaatcag tcatatgtag
      601 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
      661 attttacttt tgaaaaaatc ggggaggtat agaaaatgac agatatcgta aaaataaaac
      721 aaagcggtgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
      781 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
      841 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
      901 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
      961 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1021 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1081 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtgggga
//
LOCUS       DACEZN010000304         1136 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.321, whole genome shotgun sequence.
ACCESSION   DACEZN010000304 DACEZN010000000
VERSION     DACEZN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.321"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            4..963
                     /locus_tag="IXH60_002904"
     CDS             4..963
                     /locus_tag="IXH60_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1991771.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          384 a          210 c          226 g          316 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggcttt
//
LOCUS       DACEZN010000305         1116 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.322, whole genome shotgun sequence.
ACCESSION   DACEZN010000305 DACEZN010000000
VERSION     DACEZN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.322"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            89..>1116
                     /locus_tag="IXH60_002905"
     CDS             89..>1116
                     /locus_tag="IXH60_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1991772.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEK"
BASE COUNT          370 a          208 c          259 g          279 t
ORIGIN      
        1 taatatatgg agattgtgta ataagtatca aactaaaaga attatcatat attcattata
       61 tagatagtct ataaggagcg gatgatcaat ggcaaagcaa aatattgtcg ttgtaggagc
      121 aggatatgcc ggtgtttcag ctacaaaata cttggcaaag aaactgaaaa aagaagatgt
      181 agcaattact ctgatcgatc gtcattcgta ccatactatg atgaccgagt tgcatgaagt
      241 agctggcgga cgtgtagaac cagaagcaat ccaatacgat ctgcagcatt tgtttgctcg
      301 tcaaaagaat gtacaacttg tcacagacac tgtaatcggt atcgataaag aacgaaaaat
      361 cgtcaagacc aaattaggtt cctatccttt tgatcagctg atccttggaa tgggcggaga
      421 gccgaatgac tttggtacac ctggtgtcaa agcacacgga ttcacccttt ggtcatttga
      481 agattctgta aggattcggg aacatattga gaagacagta gcaaaagctg cattggaacc
      541 agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc tgtggttctg gatttaccgg
      601 tattgaaatg attggggaac tgatcgactg gaaaaatcgc ctagccaaag attataaatt
      661 agatccaaac gaaatcacac tgatggtcgt agaggcaatg ccgacgatct tgaatatgtt
      721 ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt aagcacaacg tggaattatt
      781 attgaatgca ccaatcgtag aagtggctgc tgatcatatc aaattgaaag acggacgcaa
      841 cattccgact cacacactga tttggacagc tggtgtcaaa ggaacttctg acgctgctga
      901 ctttggatta gaatcagcac gcggacagcg gttgatcgcc aatgaatata tgcaggcaaa
      961 aggctacgaa gataaaaaca tctatatcat tggtgattta gtcttttatg aagaattccc
     1021 aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa acaggacaca cagccgcagc
     1081 gaatatcgtg gcttcgatca aaggcggaga aaagca
//
LOCUS       DACEZN010000306         1090 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.323, whole genome shotgun sequence.
ACCESSION   DACEZN010000306 DACEZN010000000
VERSION     DACEZN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.323"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(114..1061)
                     /locus_tag="IXH60_002906"
     CDS             complement(114..1061)
                     /locus_tag="IXH60_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ1991773.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
BASE COUNT          373 a          185 c          169 g          363 t
ORIGIN      
        1 acaagcttct caaaaaaagg gattttgata tccaataaaa agagtgaatc attgtactgc
       61 cctccaaaaa agtagtacca aaaatctaaa ttttttggag gattttacat gactcactct
      121 ttttttatca aagaaagtat tttccaagca gttttttcat ccaaaaataa atctgtaaaa
      181 aaattaccat tcaaggcgcc taaacaactc tccgctttga acttactttt acaaatacca
      241 attcgttttg gaatatcgaa aatttctttt aaagaaagac caaaagtctt atcctcagat
      301 gctttgatga attcaccatt gatattgaag ggtcttccgt aaagaaatcc aacagtatcc
      361 gtcatgcttg gaaataggat tttacgatgc ttgttccagg gttcgacaga atcaatagag
      421 gataaggtgc caattccaga aattaacata tctgcatgcg aagcggttgc taaggtatta
      481 gctaaagaag gttctagagc cattagttca cgtgcagttt cattgatgac atataaagga
      541 atactgagag tcaaataaga agactcataa cgttcagcag ctttttgaac taatcgtgtg
      601 gagccagcta aggaattata ttttccgatt tcgccaatga attgagtaaa aattaattct
      661 tcacgagcac ccattttaaa ctgatcaatc actttgtaaa cggtatctcc ccaagatagt
      721 gcaaccacat tggctttctt aattaattct tgaatgtaat cggcagcaaa ttttgaaaat
      781 aataaatcgt gttgtgataa gtcttcacta ctttttaata tatagatatt cgttttaaag
      841 taatttttga agatttcttc tagctcataa ttcctagcat acggagaatt aattgaaata
      901 ctaactaatt gtttttcacg tgcatcttca atatatttta aagctttata tctggtgatc
      961 ccatattttt tagaaatttc actaatattg atatcctcga gataatagtc ttcacaaatt
     1021 tttacaagga gtgattcata gtcagagaat tccagatgca tagtatagct cctaactttt
     1081 tcaaatagat
//
LOCUS       DACEZN010000307         1077 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.324, whole genome shotgun sequence.
ACCESSION   DACEZN010000307 DACEZN010000000
VERSION     DACEZN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.324"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(127..200)
                     /locus_tag="IXH60_002907"
     tRNA            complement(127..200)
                     /locus_tag="IXH60_002907"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ile,seq:cat)
     gene            complement(755..1069)
                     /locus_tag="IXH60_002908"
     CDS             complement(755..1069)
                     /locus_tag="IXH60_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991774.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          402 a          170 c          166 g          339 t
ORIGIN      
        1 ctttcattaa tccataaaag gatacataaa aaagccactc atttgagtgg caatgaagaa
       61 aagctttagc ttgtataata ctcttcaaaa aattctaaca cagaacgatt caattggcta
      121 cattaatgta ccctgtagga ctcgaaccta cgaccggacg gttatgagcc gtctgctctg
      181 accaactgag ctaagggtag tggttgttgc cacataaagc cataaacaat caaccagtag
      241 aatgcgtggc aacaaacctg ttatcgcata tcttggagtg tgactattta tgagtgatag
      301 tgaagatatg cgtctccata tatgtatggt tgagacagat aaacatgtat gaaaaacgtt
      361 gttaaatcaa cgttttttta ttttggtcaa ggagagaaaa gtagagaaat acaaaatttt
      421 ttgcattgat tttgcattca aaaaaacatt caaacattta gaaatcccca atacgtattc
      481 aggaaaatct attttaaggt ttacaaaaag aacttttgtt cgtacactct ttttgaggtg
      541 aacttttatg ctgatggaag gaaaaacaca attatggttt aagcatgttc cttcaaataa
      601 gttttttaat tataaactct agagaaatta attcatccta acataaaata attgttcact
      661 caaaagtttt tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga
      721 agtaataatt cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa
      781 acacaagaag tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat
      841 ttgacatgga aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg
      901 aaataacccc tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac
      961 ttcgtttggt tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta
     1021 gaagtaataa tattgctatt attaagataa aattactgta aaaatccaaa agctatc
//
LOCUS       DACEZN010000308         1073 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.325, whole genome shotgun sequence.
ACCESSION   DACEZN010000308 DACEZN010000000
VERSION     DACEZN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.325"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>1073
                     /locus_tag="IXH60_002909"
     CDS             <1..>1073
                     /locus_tag="IXH60_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1991775.1"
                     /translation="KRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFR
                     RYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIATV
                     GVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQ
                     MGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVL
                     PRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPI
                     TDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLD
                     KIIDKIRQKYGFTSIVHASSMLE"
BASE COUNT          382 a          179 c          229 g          283 t
ORIGIN      
        1 aaaagcggta tggtattaca aatgtgagta gaccacgtga cttaccacaa ccatttccta
       61 aaacactaca cgttgttcca ccacgtatga acctatatat caagcgaaat atgcaggtaa
      121 ataatatttt cagaagatat gtggctgatg aagatctact gatttactcg atcgatgaat
      181 cgattttaaa agtgacaaaa tcactgaatc tttttacgac tgaagaaaca cgaagtcagc
      241 gaaggaagaa gctcgctcaa atgatccaag aacgtattaa agaagagtta ggtttgattg
      301 ctacggtagg cgtcggcgat aatccccttt tggctaaatt agccttagac aatgaagcaa
      361 agcataatga aggatttatt gcggaatgga cgtacgaaaa cgttcctgag aaagtatgga
      421 atattcccga aatgacggac ttttggggaa ttggttcgcg aatgaaaaaa aggctcaacc
      481 aaatgggaat tttgagcatt agagaccttg ctaactggaa tccttatacg attaagaata
      541 gattaggtgt gattggatta cagctgtact tccatgctaa tgggatcgat cgaacagata
      601 tagcaatccc accagaaccg acaaaggaaa aatcatatgg aaattcacaa gtgttaccac
      661 gagattatac tagaagaaat gaaatcgagc tggtggtaaa agaaatggcg gaacaagtag
      721 cgatacgcat taggcagcat aactgtaaaa caggttgtgt ccatttacac attggcacgt
      781 cgatcttaga aacgagacca gggttttcac accagatgaa gatccctatc acagataata
      841 caaaggaatt gcagaattat tgtcttttct tgtttgataa gtattatgaa ggacaagaag
      901 taaggcatgt tggaataacc tatagcaagc ttgtgtacac cgattcgctt cagctggatt
      961 tatttagtga tccacaaaag caaattgacg aagaaaattt agataaaatt attgacaaga
     1021 ttcggcaaaa atatggtttt acgtcgattg tacatgcttc ttcaatgtta gaa
//
LOCUS       DACEZN010000309         1058 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.326, whole genome shotgun sequence.
ACCESSION   DACEZN010000309 DACEZN010000000
VERSION     DACEZN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.326"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(36..992)
                     /locus_tag="IXH60_002910"
     CDS             complement(36..992)
                     /locus_tag="IXH60_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991776.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACEZN010000310         1035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.327, whole genome shotgun sequence.
ACCESSION   DACEZN010000310 DACEZN010000000
VERSION     DACEZN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.327"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>1035)
                     /locus_tag="IXH60_002911"
     rRNA            complement(<1..>1035)
                     /locus_tag="IXH60_002911"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACEZN010000311         1018 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.328, whole genome shotgun sequence.
ACCESSION   DACEZN010000311 DACEZN010000000
VERSION     DACEZN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.328"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>1018
                     /locus_tag="IXH60_002912"
     CDS             <1..>1018
                     /locus_tag="IXH60_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1991777.1"
                     /translation="PKTLHVVPPRMNLYIKRNMQVNNIFRRYVADEDLLIYSIDESIL
                     KVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVGVGDNPLLAKLALDNEAK
                     HNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIK
                     NRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMA
                     EQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKY
                     YEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEENLDKIIDKIRQKYGFTSIVHA
                     SSMLE"
BASE COUNT          365 a          168 c          215 g          270 t
ORIGIN      
        1 tcctaaaaca ctacacgttg ttccaccacg tatgaaccta tatatcaagc gaaatatgca
       61 ggtaaataat attttcagaa gatatgtggc tgatgaagat ctactgattt actcgatcga
      121 tgaatcaatc ttaaaagtga cccgatcact gaatcttttt acgactgaag gaacacgaag
      181 tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag agctaggatt
      241 gattgctaca gtaggtgtcg gagataatcc ccttttggct aaactagcct tagacaacga
      301 agcaaagcat aatgaaggat ttgttgcgga atggacgtac gaaaacgttc ctgaaaaagt
      361 atggaatatt cctgaaatga cggacttttg gggaattggc tctcgaatga aaaaaagact
      421 caatcagatg gggattatga gcattagaga tcttgctaac tggaatcctt atacgattaa
      481 gaatagacta ggtgtgattg gcttacagct gtacttccat gctaacggaa tcgatcgaac
      541 agatatagca atcccgccag aaccgacaaa ggaaaaatct tatggtaatt cacaagtgtt
      601 accacgagat tatactagaa gaaatgaaat cgagctggtg gtaaaagaaa tggcggaaca
      661 agtagccatt cgtatcaggc agcataattg taaaacagga tgtgttcatt taaacattgg
      721 tacgtcgatc ttagaaacga gaccaggatt ttcacaccag atgaaaatac ctatcacaga
      781 taatacaaag gaattgcaga attattgtct tttcttgttt gataagtatt atgaaggaca
      841 agaagtaagg cacgttggaa taacctatag caagcttgtg tacaccgatt cgcttcagct
      901 ggatttattt agtgatccac aaaagcaaat taacgaagaa aatttagata aaattattga
      961 caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcaa tgttagaa
//
LOCUS       DACEZN010000312         1003 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.329, whole genome shotgun sequence.
ACCESSION   DACEZN010000312 DACEZN010000000
VERSION     DACEZN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.329"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            129..>1003
                     /locus_tag="IXH60_002913"
     CDS             129..>1003
                     /locus_tag="IXH60_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1991778.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDF"
BASE COUNT          360 a          153 c          189 g          301 t
ORIGIN      
        1 aatatcccat attttaaaat aaaagatagc gtaaaatatt ttgtgtaaat agaaatttag
       61 ggtagaaaaa aaagtccatt ctgtaaaatt aaagttacca cacagaaatt ttataggagg
      121 actttctcat gactaaattt actatagaaa ttatgcaaac actactcaat aaaggggatt
      181 tagatgaatt atttcaggat catttagagc gagcgattaa ttcacttcta caagcagagc
      241 taactgcttt tttagattac gaaaaatatg atcgaaatgg attcaattca ggaaattccc
      301 gtaacgggaa ttattcacgt acatttaaaa cagaatatgg cgagttgaat ttaactattc
      361 ctagagatcg gaatggcgaa ttttctcaac aaacactacc agcatataag cgaaccaatg
      421 attcattaga aacgaccatt atccaattat ttcaaaaagg cattactatg gctgaaatct
      481 ccaaattaat tgaaaaaatg tatggtcacc actatacgcc acaaacaatc tccaacatga
      541 gtaaattggt ggctgaagat gttttagctt ttaaagaaag aacgttagaa gccaattatt
      601 ccgttatatt tatggatgcg acgcatatcc ctgtgaagcg acagactgtt tcgaaggaag
      661 cagtgtatat tacgattggt attcgtttgg atggaaccaa agaagttctt gggtttacta
      721 ttgccccaac tgagtctgct tatatttgga aagaagttct tcaagatctt agaaaacgtg
      781 ggttagaaga agttttatta gtagtgacag acggattaag ctgtattgaa gaaagtatcc
      841 atagtgtgta tccgaatgcc caatttcaac aatgttgtgt gcatgtatct agaaatatcg
      901 ctcataaagt tcgtgttcga ggtcgaaaag aaatttgtga ggatttcaaa ttggtttacc
      961 aagcgaattc aaaagaagag gcattggatc acatcgactt tat
//
LOCUS       DACEZN010000313          999 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.330, whole genome shotgun sequence.
ACCESSION   DACEZN010000313 DACEZN010000000
VERSION     DACEZN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.330"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..912
                     /locus_tag="IXH60_002914"
     CDS             <1..912
                     /locus_tag="IXH60_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1991779.1"
                     /translation="IGSTLANHLGEEHVVIVVDDLSMGKVENLDMTKNITFIEGDVAE
                     KSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMILELIRKYQPDLK
                     RIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYCHLYDVPGSAVRF
                     FNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSRDFVYIDDVIQAL
                     LLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEERSGDIHDSLADI
                     TKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          326 a          154 c          212 g          307 t
ORIGIN      
        1 attggttcta ccttagccaa tcatttagga gaagaacatg tggtaatcgt cgtcgatgac
       61 ttatcaatgg gaaaagtaga aaatcttgat atgacgaaaa atatcacttt tattgaggga
      121 gatgtcgctg agaaatcctt gatggaagag atcatgaaag ctaataaatt tgattatatc
      181 tttcatttag cagcagttgc tagtgtggca gattcagttg cccgaccagt agaaacacat
      241 cgtgtgaatt ttgaaagtgt attaatgatt ttagaattga tccgcaagta tcaacctgat
      301 ttaaaacgga tcgtgtttag ttcttcagcg gctgtttatg gggatgaacc gactttacca
      361 aaaaaagaag agtccgtgat tcgcccattg acgccttatg cggttgataa attcgctgca
      421 gaacaatatg tgctagatta ttgtcattta tatgatgtac ccggtagcgc agttcgtttt
      481 ttcaatgtct atggtccaaa tcaaaatccg aattctccat acagtggtgt gatttctatt
      541 ctcgtagatc gctataagaa acaaatcgta ggagaagcaa cttcatttac attatttggt
      601 gatggaagcc agtctagaga ctttgtttat atagatgatg taatccaagc attactttta
      661 gttgcgaatg aggaagctgc actggggaaa caattcaatg ttggaacggg taaagcaacc
      721 acacttttgg aattgattca tgcaatcaat gaaattctcg gtgtagaatt gacacttgaa
      781 tacaaagaag agcgttcggg tgatatccat gactctctag cagatattac taagatccag
      841 tcaattggct atcaaccgaa atatggtatt ttaagtggaa tgactaatta tttgaaaact
      901 gaaatcaatt aaaacatact aagataagat tagagggtaa attcaggctc tttgtcaata
      961 aggactgatg agttttgtaa aatcaaatct gggatagaa
//
LOCUS       DACEZN010000314          993 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.331, whole genome shotgun sequence.
ACCESSION   DACEZN010000314 DACEZN010000000
VERSION     DACEZN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.331"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            36..>993
                     /locus_tag="IXH60_002915"
     CDS             36..>993
                     /locus_tag="IXH60_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1991780.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
BASE COUNT          354 a          178 c          189 g          272 t
ORIGIN      
        1 agttccgacg aaagaattat aaggagtgag tacaaatgac ctataaccat cttacaccaa
       61 ctgaactcgt aatgatagaa gcttatttca atcaatcaca acctgtttca aaagtagcca
      121 atctgttaca acgttctcga caaactattt ataaggtcta tcgttttctt aaatctggtg
      181 gaagcgcaat gagctactat aaacaatata agaaaaacaa acgtaactgc ggaagacatc
      241 cgattgtttt acctgatgag caacaggaat acattcaaaa aaaggttgtt caagggtggg
      301 ctcctgatgt ccttattggt cgagcagagt ttcctattgc ttgttcggta cgcacactgt
      361 atcgaatgtt taaagataaa acctttgaca ctcacgattt accaatgaag ggaaaaagaa
      421 aaccaaacgg tcataaagaa aaaagaggaa aacaagggtt tcgccggtca atccgagaca
      481 gaaagacaga ctatacccag tttgatcatg aatttggtca cctggaaggt gacactatcg
      541 tgggtggaaa acataaaagc gcggtcatca cattagttga acggctatct aaagtcatca
      601 ttaccttgaa acccgaaggc agacaggcta aagacattga aaataggctg aatcaatggt
      661 ttcgttgtgt cccaaaaaac ttatttaaat ccatcacatt tgattgtgga aaagaattct
      721 caaattggaa aaagattagt aataccaacg atatttcaat ttattttgcg gatccaggaa
      781 caccttctca acgaggattg aatgaacatt caaatggctt attacgtaaa gatggtttgt
      841 ataaagctat ggattttaat ggtgtatctg aaacctttat ccaatcggtg gcatcgaaac
      901 gaaatcatat tcctagaaaa tcattacact atcggacacc aatggaagta tttttgagtt
      961 acgtcacaaa agatgatttg tctaacttaa ttt
//
LOCUS       DACEZN010000315          941 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.332, whole genome shotgun sequence.
ACCESSION   DACEZN010000315 DACEZN010000000
VERSION     DACEZN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.332"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(629..922)
                     /locus_tag="IXH60_002916"
     CDS             complement(629..922)
                     /locus_tag="IXH60_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1991781.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          282 a          161 c          146 g          352 t
ORIGIN      
        1 ttgaagtatt gcttaaattt ggaagctatg agggaagaat aaaagagtta ggacgggatt
       61 atctactatt tttctttgtt ttaaatattt gttatctttg aacataaaag agaaatgtgt
      121 cgactgcagt gttgtgtccc tcatcacgaa taaacggtgt tcaactagct gatcttgtat
      181 tcacttaaaa aatggtaaag tacgagttgc tattccccat tttcaatttt ctaacgctca
      241 ggacaaaata ctgttttcac aacctcattg gaataaaagc atatatattg tttcaagaac
      301 gaaatcttgt tatcttttta aagggcatct gtccaaaatc taattttagg aggtacgttt
      361 tggtatttat tcgtacaaat aaaagagaat tatctttatt catgtaacgc atccaattga
      421 ttttttcatg tacgctcctg gaacgatttt actgacgatt gcttatttaa ctgcgaatcg
      481 ctcattagct tttatcatag ctatccatat tttgaataac gttcttggct ttgtattata
      541 gatacagatg acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc atacttgaac
      601 ttttttcaat gagtataaat taaaacttct aattttcaat tcccatcatc tgatatgccc
      661 gaccgtctga acttcttttg aaaaagccaa atgtgattag ataacgtctt tgttcgacat
      721 aatcgccact agaaaatacg ttcagcagta tcgaattaac ctctgtttct gtataagtca
      781 ctccatggtc aaagcgtttc aagacttctt tcaataggat gtatttcttt tttgttgaac
      841 ttggtacttg ttttactttt ccatcattca tgaatgtgtc taaaactttc atcttttctt
      901 gctccgtata agtataaacc atcttctttc tccttttgat t
//
LOCUS       DACEZN010000316          890 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.334, whole genome shotgun sequence.
ACCESSION   DACEZN010000316 DACEZN010000000
VERSION     DACEZN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.334"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..888
                     /locus_tag="IXH60_002917"
     CDS             <1..888
                     /locus_tag="IXH60_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1991782.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACEZN010000317          889 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.335, whole genome shotgun sequence.
ACCESSION   DACEZN010000317 DACEZN010000000
VERSION     DACEZN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.335"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            28..>889
                     /locus_tag="IXH60_002918"
     CDS             28..>889
                     /locus_tag="IXH60_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1991783.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVAS"
BASE COUNT          305 a          154 c          168 g          262 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatcta
//
LOCUS       DACEZN010000318          889 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.336, whole genome shotgun sequence.
ACCESSION   DACEZN010000318 DACEZN010000000
VERSION     DACEZN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.336"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..778
                     /gene="tnpB"
                     /locus_tag="IXH60_002919"
     CDS             <1..778
                     /gene="tnpB"
                     /locus_tag="IXH60_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1991784.1"
                     /translation="DSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYISLL
                     CKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRAL
                     LAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNT
                     KGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGK
                     KSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          332 a          138 c          160 g          259 t
ORIGIN      
        1 tgatagtaaa ttcatcaaag ttcctaaatt gaagtcatta gtaagaatta agcttcatag
       61 acaaccaaaa gggattatca aatctgctac aatatcacgt cattctagcg gtaaatacta
      121 tatttctttg ttgtgtaaag aagaaattat cgaattacct aaaactaatt ccacaattgg
      181 tattgactta ggtattatgg actttgcgat tctttctgat ggacaaaaaa ttgataataa
      241 taaattcacg tccaaaatgg aaaagaaact aaagcgtgaa caacgcaaat tgtctaggcg
      301 tgctttattg gctaaaaata aaggtaccaa tctatttgaa gctaaaaatt atcaaaaaca
      361 aaaacgcaaa gtagctagat tgcatgaaaa agtaatgaat caacgtactg attttctgaa
      421 taagttgagt acagaaatga tcaaaaatca cgatattatc tgtattgaag acttaaacac
      481 aaaaggtatg ttgcgaaatc ataaattagc taaaagtatt tctgatgttt cttggtcaaa
      541 gtttgtgact aaattacaat acaaggctga ctggtatgga cgaaaaatca tcaaagtaga
      601 taaatggttt ccgtctagtc agatttgttc agagtgcgga cataaagatg gcaagaaatc
      661 tcttgaaatt cgagaatgga cttgtcctgt ttgtcatact catcatgatc gtgatatcaa
      721 tgctagtatc aatattctga ccgaaggtct aagactacat tctatgggat tggcttagat
      781 ccaatcatca agaaccgtag gaactacggg gatagcttgg taaataagag acaccgctgt
      841 tagtaaagaa atacgctatc aagtatgctc tattcccaag aagctccca
//
LOCUS       DACEZN010000319          856 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.339, whole genome shotgun sequence.
ACCESSION   DACEZN010000319 DACEZN010000000
VERSION     DACEZN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.339"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            278..787
                     /locus_tag="IXH60_002920"
     CDS             278..787
                     /locus_tag="IXH60_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1991785.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
BASE COUNT          331 a          112 c          134 g          279 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaat
//
LOCUS       DACEZN010000320          854 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.340, whole genome shotgun sequence.
ACCESSION   DACEZN010000320 DACEZN010000000
VERSION     DACEZN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.340"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            223..854
                     /locus_tag="IXH60_002921"
                     /pseudo
     CDS             223..854
                     /locus_tag="IXH60_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          282 a          163 c          163 g          246 t
ORIGIN      
        1 acacacacat gctagcttgc ttttagcaag ctagtgcaaa tattaaagat gtgcaagagc
       61 gtttaggcca ctcttctatt caaatcatta tggatctata tattcatatt acagacaaaa
      121 gaaaagaaga aaccgtagcg caattcgcaa aatataggct ctataattta tgggactgat
      181 gaatcaaaat tgattcttca gtcccattgt tattttaggt atatgattcg tacattgaaa
      241 gagttgaaaa actatcttcc gaagccgtca aaaaggaccc tgccaaaagt attgatggtg
      301 gatgaatttc gttcgcacgc ttcaattgtg gataaaatga gctttatttg tgcggatggc
      361 gagactggaa aactcattga tattttgcct acacgaaaat tgtctcgatt aacacactat
      421 tttagtcgct gtgcaaatcc tgaagcggtg gaatatttag tgacagatat gaacgccgcc
      481 tacttccaac tgacaaaacg tgtcttacca aatgcgaaag tcgtcattga ttgttttcat
      541 atcatcaaac atttgaatca ggcgttcaat gattttcgtg tacaagaaat gaaaagatta
      601 caccagttag ggcaacgacg ggaagccgag aaagtgaaaa agaactggcg ctttcttcta
      661 aaaaaccgtc aaaaaatcaa tcactatgat tacaaaacat ggcgcagttt tcgtgcacct
      721 aaatacccgc tactcactga agcaatgatg attgatcgat tgctggcatt ttcaacgcct
      781 ttaaaggacg cctatacttt tttccatgag ctgaccgaag cctttagaga caaggaatca
      841 gacttatttt ttac
//
LOCUS       DACEZN010000321          846 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.341, whole genome shotgun sequence.
ACCESSION   DACEZN010000321 DACEZN010000000
VERSION     DACEZN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.341"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          298 a          116 c          149 g          283 t
ORIGIN      
        1 atgagctaat tttatgatta aatggtaagt gtttccttgt ttgttttagt ttaaactagt
       61 gtgtaattta aaaatctata tggaagggtc tgtggggtaa tacttaaata aaggctagga
      121 acctgataac atcaaggttc ctagcctttt tataataaaa tgaagcgaaa ataatttctt
      181 ttaaagagcc gagattatca gctatataag agtgaatttg atgcaaaaaa cagagccgaa
      241 acagtcttgg tgttcgtagc tctgcttata tgtttcgaat ttaagacaaa gagttggcaa
      301 aagatgttga taaggaagat aagataactg tgttatataa actaggtaac taactgtaac
      361 aagctaattt taagagattc ttattagaag acgtttagga atataactaa gaaaatgatt
      421 atacaggtaa ttgtcatttc aataatgttt tctaattttc gcgtctttgt tttaagaaaa
      481 aggccagaat cagttggaaa aaagttcgta cgatagtgaa gaggggcaaa atttcctact
      541 aatattccca ctaacaagag ataaaacggc caggttggtg tgattcctct ataaaaaaat
      601 gcccatgcta ccgaaatccc atagattaac aactgaacaa tttttacaat gaaaaaattg
      661 ttctgccatt actcaaagtc tcttcctcct tgtatcgttt atttgtttca ttttatcatt
      721 atgataggcg cttgaacaat ccattttgta ttttattgag tactcttgga taaactacca
      781 gcgattatca tatttattta tgttactgta agccatcata taaaagtaaa tgagcagatc
      841 atagaa
//
LOCUS       DACEZN010000322          828 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.343, whole genome shotgun sequence.
ACCESSION   DACEZN010000322 DACEZN010000000
VERSION     DACEZN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.343"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..589
                     /locus_tag="IXH60_002922"
     CDS             <1..589
                     /locus_tag="IXH60_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991786.1"
                     /translation="YCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKC
                     IFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPF
                     EVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLT
                     NIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            576..>828
                     /locus_tag="IXH60_002923"
     CDS             576..>828
                     /locus_tag="IXH60_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1991787.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINE"
BASE COUNT          301 a          110 c          123 g          294 t
ORIGIN      
        1 ctattgtccg gaattgttac tttatgatgt aaaaaatata aaaaaaattc ccaaaataga
       61 tgaaaacagt aaattgatga aaaacataca atcaatcttt gggatagaag aagtttttgt
      121 cggagataaa tgtatctttt ttgatcaacc tttattatct gatggttatg gcatagatga
      181 aacagatatt ttcgctaaag taaatagtat attttctgga aaaaaagaaa ctttaattaa
      241 gcttcatcct agagcaagga caaatcgtta tggtgaaaaa aacatattga attccagttt
      301 accgtttgaa gtcttgctac ttgaaaatga cttctcttca acacttttga ttagtcctat
      361 atctacaatt tctttttcac catctatgat gtttgaaaag acgatcaact ctattatgtt
      421 ggctaaatta attttaaatg aatatacagc attatctgaa gataaaaaag aaacattgac
      481 aaatattgtt cttttctgtg aacattataa ccgtctatca gaagaaaaaa ttttactccc
      541 agaaaactgg gcagaattaa gaaagttggt agagtatggg attatttaac acactttcca
      601 agtcaaaatt agttaagtcc agtttctggt atactatagg gaattttttt attaagggaa
      661 tttcattcat aactattcct atttttgtaa atattactac tcaagcagaa tatggtttga
      721 ttaataattt cacttcattt gtatcaattt tttcgattct tgttgggcta agcctaaatt
      781 cggcaattaa taatgcaagt tttgactata aagatgacat aaatgaat
//
LOCUS       DACEZN010000323          821 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.344, whole genome shotgun sequence.
ACCESSION   DACEZN010000323 DACEZN010000000
VERSION     DACEZN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.344"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..405
                     /locus_tag="IXH60_002924"
     CDS             <1..405
                     /locus_tag="IXH60_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1991788.1"
                     /translation="VIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMD
                     RIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEI
                     EKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            477..>821
                     /locus_tag="IXH60_002925"
     CDS             477..>821
                     /locus_tag="IXH60_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991789.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          346 a           98 c          136 g          241 t
ORIGIN      
        1 gttatacgtg aaaatgtatt acaaagagaa ttaaatacta gagccggtta tgtgtatgta
       61 attagtaata ttgggtcttt tggtgaagac atatataaaa ttggtatgac tagaagatta
      121 gaacctatgg atagaataaa agaattgagt agtgcatcgg ttccatttga atttgacgtt
      181 catgcgatga ttttttcaga ggacgctccg tctttggaaa ctaaattgca taatcatttt
      241 agaaaacaag aagttaataa aattaatcaa agaaaagaat ttttcaaagt ttcactagac
      301 gaaatagaga aagttgtttt agaaaattat aatggaacag taacatttac aaaattagct
      361 aaagctgaac aatatagacg atctttagaa ttgtcaaaag attaaatata taaaaagcct
      421 tcgggctttt tcttttaaca attaatagga acgtatattc gaaaggaata gaaataatgt
      481 ggttagaaaa aataaatgac aaaaaattta agtacaccga acgttatacg gatcccttaa
      541 ctgaaaaaaa gagaaaagtg tccgttactt tatctagcaa ttcacgccaa gcttggaatc
      601 aagctaactt acttttgaat gaaaaaatcg cagaaaaaat aaaacgaaat gaagaattac
      661 cactaacttt tggagaatta aaaactaaat gggatgaaaa atacaagcca actgtaaaag
      721 aaagttccta tcgaacaact caggtttatc tatcattaat ttcaaaatat ataaaagatg
      781 atgtacttgt aaaaaacgtt aatagtaatc ttatacaaga t
//
LOCUS       DACEZN010000324          820 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.345, whole genome shotgun sequence.
ACCESSION   DACEZN010000324 DACEZN010000000
VERSION     DACEZN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.345"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            19..>820
                     /locus_tag="IXH60_002926"
     CDS             19..>820
                     /locus_tag="IXH60_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991790.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          252 a          141 c          185 g          242 t
ORIGIN      
        1 ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct caacttgacg
       61 agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa aaagaaagac
      121 tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag tcgagacggg
      181 gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga tcaacggggg
      241 ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga aaatatcgcc
      301 gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc agacggctcg
      361 attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc ggatttggtt
      421 ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct tagtgtttcg
      481 atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg agaaatcttc
      541 aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg aaaaacgtta
      601 tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt cgtcactcaa
      661 aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa gtttgctttt
      721 cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt ggatcaggag
      781 gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DACEZN010000325          790 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.346, whole genome shotgun sequence.
ACCESSION   DACEZN010000325 DACEZN010000000
VERSION     DACEZN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.346"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..782
                     /locus_tag="IXH60_002927"
     CDS             <1..782
                     /locus_tag="IXH60_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1991791.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc
//
LOCUS       DACEZN010000326          774 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.347, whole genome shotgun sequence.
ACCESSION   DACEZN010000326 DACEZN010000000
VERSION     DACEZN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.347"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            350..748
                     /gene="spxA"
                     /locus_tag="IXH60_002928"
     CDS             350..748
                     /gene="spxA"
                     /locus_tag="IXH60_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1991792.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          289 a          107 c          137 g          241 t
ORIGIN      
        1 tttaaaatga cagaaaacat tactattaaa gaaataaaaa acataaaaaa tcttaagaaa
       61 tcggtttcat ttagcttttg agataaaata atagtacagt tttatacgct gtttcatatg
      121 tttagaaaat ttgaattaga tttgaaaact ctaattttta aatgataaaa aaagcgagaa
      181 tgctggtttt actgtatatt aaccttagat aactttcatt tttttaaaaa aattatgaaa
      241 aaaaggtagc tatttaaaag ttgattatgt ataatagaaa cagatgggag attattatga
      301 tcttctgtta caaaaaagtg attcttatca cgaaaaggag tggtgcacgt tgttaacatt
      361 atatacttcc ccaagttgta cttcctgcag aaaggctcgt gcgtggttgc aagaacatca
      421 gattccattt gttgaacgta atattttttc agaaccattg aacagttctg aattaaaagc
      481 gattttacaa atgacagaag acggaacaga agaaatcatc tcgacacgtt ccaaagtatt
      541 ccaaaaactg aacatggatt tagatgatct gccgttacaa gaattattgg aacttgtgca
      601 aaacaatcct ggattgcttc gccgtccaat catgattgat gataaacgtc tgcaagttgg
      661 ttttaatgaa gatgaaattc gtcggttcct accgcgcgat gtacgacaat tagaattacg
      721 tcaagcacaa ttgatggctg gattatagaa ggataatacg aaacttggaa aaga
//
LOCUS       DACEZN010000327          771 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.348, whole genome shotgun sequence.
ACCESSION   DACEZN010000327 DACEZN010000000
VERSION     DACEZN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.348"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            116..>771
                     /locus_tag="IXH60_002929"
     CDS             116..>771
                     /locus_tag="IXH60_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAQ1991793.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     "
BASE COUNT          282 a           97 c          126 g          266 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tatcaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa a
//
LOCUS       DACEZN010000328          763 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.349, whole genome shotgun sequence.
ACCESSION   DACEZN010000328 DACEZN010000000
VERSION     DACEZN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.349"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(237..761)
                     /locus_tag="IXH60_002930"
     CDS             complement(237..761)
                     /locus_tag="IXH60_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991794.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          261 a          101 c           85 g          316 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttacctttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
      541 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa aatctaaact attaaaaaca
      601 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
      661 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
      721 agctcagttt gtttatcatc ctcaataatt ttataaatca aac
//
LOCUS       DACEZN010000329          748 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.350, whole genome shotgun sequence.
ACCESSION   DACEZN010000329 DACEZN010000000
VERSION     DACEZN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.350"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            554..>748
                     /locus_tag="IXH60_002931"
     CDS             554..>748
                     /locus_tag="IXH60_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1991795.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT          271 a          136 c          115 g          226 t
ORIGIN      
        1 gacactcctc ttaatataca gtcctattgt ataaagtgtt actatataca tagtaactgt
       61 atacataaaa aaagtcaagt attagaaaaa agttcaaaag atcaagtgtg cgcttcgctc
      121 ccactcccct cgacgtttta gtagcctttc cctcacttcg ttcagtccaa gccaactaaa
      181 aagttttcgg gatacattct ccttctcttc ctaataatca attaaaattt taatctttat
      241 atttctgcta atcactcagg aaacagtaca gaaaaacaaa cacgtatcat agatataaat
      301 gtaatggcat agtgcgggtt ttattttcag cctgtatcat agctaaacaa atcgagttgt
      361 gtgtccgttt ttgggcgttc tgctagcttg tttaaagtct cttgaatgaa tgtatgttct
      421 aagtcaaaaa actctgacag cgcctttata tagataaagt ccgtataatt gtgtaaaagt
      481 aaaaaggcca tataacagtc cttttacggt acaatgtttt taacgacaaa aacataccca
      541 ggaggacttt tacatgaccc aagtacattt tacactgaaa agcgaagaga ttcaaagcat
      601 tattgaatat tctgtaaagg atgacgtttc taaaaatatt ttaacaacgg tatttaatca
      661 actaatggaa aatcaacgaa cagaatatat tcaagcaaaa gaatatgaac gaacagaaaa
      721 ccgacaaagt caacgaaatg gctattat
//
LOCUS       DACEZN010000330          706 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.352, whole genome shotgun sequence.
ACCESSION   DACEZN010000330 DACEZN010000000
VERSION     DACEZN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.352"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(437..600)
                     /locus_tag="IXH60_002932"
                     /pseudo
     CDS             complement(437..600)
                     /locus_tag="IXH60_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
BASE COUNT          237 a          123 c           78 g          268 t
ORIGIN      
        1 atcttaatta tctatccatt tcatcctacg cattcttgcg taaatacaat tcactctgtt
       61 gagctgatat aaatcattgg tggtacaaag ataaaatgat aactgtttct atacctccaa
      121 aaattccagt aaatatatct atagatagtt tcaaaaaaaa ttgtcttagt ttcataatat
      181 ttaagatagt ggtacatact ttataacaac gtacttttcc aaatattatt ttccgttatg
      241 aacatccaat atattacaaa gtttaaaagt ttagatgaac tataatcttc atttctgcac
      301 agtggataat aaattcttat agataaaaaa tagcctcttc taaagtcaca aataccttac
      361 tcccacattt taagtaaaca ctctgcagca ttctctttgt tataaaatga tagatattcc
      421 tcaattttat ctcttattat gttattatca gttgatacca ttccattaat gtttcagaat
      481 aattacaata ttttgggacc ggataactcc cttttgctcg taaccactca tatccgttag
      541 gattcactaa aagtttaccg tttatttttc gattcttttt ttgaaccgtc ccatgaacat
      601 accgattcaa caagctaaat tataaaaatt ggtgtgctgt cattattttt tggaaaattt
      661 attaatggct atcaatatac ttttgtatca tactaaatag ctcgtc
//
LOCUS       DACEZN010000331          706 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.353, whole genome shotgun sequence.
ACCESSION   DACEZN010000331 DACEZN010000000
VERSION     DACEZN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.353"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            1..316
                     /locus_tag="IXH60_002933"
                     /pseudo
     CDS             1..316
                     /locus_tag="IXH60_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            374..>706
                     /locus_tag="IXH60_002934"
     CDS             374..>706
                     /locus_tag="IXH60_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991796.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFIS"
BASE COUNT          229 a           99 c          101 g          277 t
ORIGIN      
        1 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
       61 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      121 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      181 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      241 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      301 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      361 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      421 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      481 agaattacta tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt
      541 taactttaaa aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat
      601 tctactttgt acaaaaatat ttccatggtt tgccttacaa aatactcctt taaatattat
      661 tcaagctcct tggcgtttta tgatattttc aacattgttc atatct
//
LOCUS       DACEZN010000332          702 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.354, whole genome shotgun sequence.
ACCESSION   DACEZN010000332 DACEZN010000000
VERSION     DACEZN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.354"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(17..538)
                     /locus_tag="IXH60_002935"
     CDS             complement(17..538)
                     /locus_tag="IXH60_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1991797.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT          193 a          131 c          117 g          261 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca ga
//
LOCUS       DACEZN010000333          680 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.355, whole genome shotgun sequence.
ACCESSION   DACEZN010000333 DACEZN010000000
VERSION     DACEZN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.355"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            39..644
                     /locus_tag="IXH60_002936"
     CDS             39..644
                     /locus_tag="IXH60_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1991798.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
BASE COUNT          227 a          118 c          163 g          172 t
ORIGIN      
        1 taagataaat ctcagcaaag gataatggag gaaagaacat gatttttgct tataacaaag
       61 cacacgtcgg agacactttg atggttatca tagccgacga taaaggaaca gaaaacactg
      121 tcgaacgtaa atggaatgtt gctcggatca aagatgaaca aggtcaagtt gttgcttgga
      181 actttttcca tatctccgat catctaacaa ttgaagggaa cggccaagta agcttaacag
      241 atgaagaatt agcgaaattg aatcgattga ttaaagaagc aggattcgaa gaacagcttg
      301 aagctgatca tgaaccgaag atcgttgtcg ggtatgtaaa aacatgcgtc ccgcatcctg
      361 actctgacca tttatcgatt actgagacag aagtggacaa tgggacagta ttgcaaattg
      421 tttgcggtgc accaaatatc gaagctggac aaaaagttgt cgttgcaaaa ccaggagcca
      481 tgatgccaga tggaatgatg atctggccag gaactttgcg aggggtagcc agctatggaa
      541 tgatttgttc tgctcgtgaa ttgcatttgc caaatgctcc agaaaaaaaa ggaatcttag
      601 tattgcctga ggatgccgta gtcggagaag tatttccaaa ataaagaggt tgtgacaagt
      661 ctttgtcaca acctcttttt
//
LOCUS       DACEZN010000334          668 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.356, whole genome shotgun sequence.
ACCESSION   DACEZN010000334 DACEZN010000000
VERSION     DACEZN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.356"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>668
                     /locus_tag="IXH60_002937"
     CDS             <1..>668
                     /locus_tag="IXH60_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1991799.1"
                     /translation="KHIDFTKSDENIYLEQTNLADKYEELFGEVVEKYNAKQKRADRK
                     IEDYLAKVRKDKKLEPQREFIVQIGILDDFQTNRADGSSTGISKQQAEQNRVIANKIL
                     VQYYKEFQERNPNLAVYNAVIHNDEISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQ
                     GIHADKDNGRVLFNNFRNQEVDSIERLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISE
                     LRQT"
BASE COUNT          252 a           96 c          144 g          176 t
ORIGIN      
        1 ataagcatat tgacttcacg aaatcggatg aaaatattta tttagagcag acgaatctag
       61 cagataaata tgaggaattg tttggcgaag tggtagaaaa atataatgcg aaacaaaaaa
      121 gagccgatag aaaaatcgag gactatttag cgaaagtgcg caaggataaa aagttggaac
      181 ctcaacgaga atttattgtg caaataggca ttttagatga tttccagaca aatcgagcag
      241 atggttcttc tactggcata tcgaaacaac aagctgaaca aaatcgagtg attgccaata
      301 aaattttggt tcagtattac aaggagtttc aagagagaaa ccctaatttg gctgtttata
      361 atgctgttat tcataatgac gaaataagtc cgcacctgca cttgaatatt gtgccggttg
      421 ctgaaggtta taaacggggt gttcaaaagc agccgagctt taataaagca ctggttcaac
      481 aagggataca cgctgataaa gataatggtc gagtgctttt caataatttt agaaatcaag
      541 aagttgactc tatagaacga ttaatgaatg attacggttg gaaaagggat attgtcggta
      601 ccaacaaaat taaagatatt cacgaataca aagaaatcat gagtgaaatt tctgaattgc
      661 gccaaacg
//
LOCUS       DACEZN010000335          660 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.357, whole genome shotgun sequence.
ACCESSION   DACEZN010000335 DACEZN010000000
VERSION     DACEZN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.357"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            240..653
                     /locus_tag="IXH60_002938"
     CDS             240..653
                     /locus_tag="IXH60_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ1991800.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          271 a           77 c           82 g          230 t
ORIGIN      
        1 cataaaaatt tattatttag attctataaa catgtgcaac ttatctaaca acaaagtata
       61 tattctacag aagtaaacaa atagcttata ttttaggatg tacacaaatt gacaagtata
      121 ataaaacttt gataaaatac aaatgttttt tatttttatg tgtgattagt tagaacttac
      181 ttaaatccta ttataatatt tttgttaatg attttaataa aatgattgga gagaaaatga
      241 tggatcaaaa agcgtttatc gaatctattg attctacaat cgatagatta aaaaaagaaa
      301 ttcgatttta taatagagta attggtatcg gaaatattat aaaaatagtc ttatcagcta
      361 gtatcccaat attaattgat caggcttccg aacacaggtc tttactattg attgtttcaa
      421 ttgcttcagc aataattaca attatacaaa gtgggatgtc tgcatttaat tatcaagata
      481 aagtacagac ttcgacagag ttattaatga aaattgaaaa agaaaagttg ttatatataa
      541 cgaaaacatc cccatataat aaaacagatg aagaaaattt ccatttaatc gttacaaccc
      601 ttcaaactga attaaatgat attatctctg attttaatca agttaataac taaaaattta
//
LOCUS       DACEZN010000336          654 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.358, whole genome shotgun sequence.
ACCESSION   DACEZN010000336 DACEZN010000000
VERSION     DACEZN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.358"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(273..563)
                     /locus_tag="IXH60_002939"
     CDS             complement(273..563)
                     /locus_tag="IXH60_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1991801.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          220 a           87 c           93 g          254 t
ORIGIN      
        1 gattattttg ttttaaagat atatatcggt tcggatataa agattattca tatgttattc
       61 gaaattaaaa taatcacttt ttattacttt aaaatttttt attaatgata agaaatatgt
      121 tttgcgagca gcaactagaa taatttaaac attactaaac tgtctatatt gttactaata
      181 attcccttct tatagtgcat tttcttagaa aaaaacaata ttacgatatc atgatattaa
      241 gatatcgtaa tattatacaa ttgtgattga tattatatat gtttttttat ttttttatca
      301 tgtttaatct tgaaggaatc atattctttt tctaaagaca ggagcttttc ataaacatca
      361 gacatgttta aactagaggc caggcttaaa tcataaacga atgaagcagg taatgatttt
      421 attctacgtt gtcgggttat ccagcttgca accgttcctt gtgggtaggc atataactca
      481 caaaattgtt ctacagtgat tcctaaattc ttgataatgt aaacattgat cgggtgcgag
      541 taaacaaatg tattgataga catggtgcgt aagcctactt tcttttatta ttgcgtttgc
      601 aataatagtt taacacacgc attattctag tatctactcc tatttgtaaa aaca
//
LOCUS       DACEZN010000337          649 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.359, whole genome shotgun sequence.
ACCESSION   DACEZN010000337 DACEZN010000000
VERSION     DACEZN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.359"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>649
                     /locus_tag="IXH60_002940"
     CDS             <1..>649
                     /locus_tag="IXH60_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HAQ1991802.1"
                     /translation="RETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKF
                     TKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYV
                     KGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVAD
                     AGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLA"
BASE COUNT          217 a          118 c          132 g          182 t
ORIGIN      
        1 tagagaaacg acggtgtttg aggatatttt tgcctatatt ttagaacaag cagttaaagc
       61 aggttttgta acagaggata atttgtatct ggattctacc cacatcaaag ctaacgccaa
      121 taaacataag tttacgaaag aaatgaccca tgatgaagcg aaagcctatc aggatgaatt
      181 agaagatgag atcaatcgtc aacgaataga ggctggaaaa cgccctttta ctttggactt
      241 agaaaaggaa gtgaagctga aagaaagaaa aataagtaaa gctgatccgg aaagcggcta
      301 ctacgtcaaa ggcgaacgag aaaaacaatt tgcttattct gcccatactt catgcgacga
      361 caatggattt attcttagca caatcatcac tcctggaaat attcatgaca gtcaagtcgc
      421 ctttcagctc gtcaaacaat cgaagagact ttttcctgaa atcaattgtg ttgtcgctga
      481 tgctgggtat aagacaccta agtttgttca ctttttgact catttgaacc ttcgaccttg
      541 tttgccttat tcaaggccca aagggaaaaa ggggctatta tcaaaaaatg agtttctcta
      601 tgacgagtat tttgattgtt atatttgtcc gcaagatcaa atgctcgct
//
LOCUS       DACEZN010000338          644 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.360, whole genome shotgun sequence.
ACCESSION   DACEZN010000338 DACEZN010000000
VERSION     DACEZN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.360"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..623
                     /locus_tag="IXH60_002941"
     CDS             <1..623
                     /locus_tag="IXH60_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1991803.1"
                     /translation="EDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGL
                     KGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTF
                     LSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRR
                     KEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          237 a          118 c          111 g          178 t
ORIGIN      
        1 gagaagacgg cactaaagaa gtactgagtt atgcgattgc tccaactgaa tcaacatacg
       61 tttggaatga gctgctacag gatattaact ccagaggagt tcaagaagtc ttgcttttta
      121 ttacggacgg cttaaaaggc atgaaagata ctatccatca aatttatcct aaagcaaaat
      181 atcagcattg ttgtatccat gtatctcgta acatcgctca taaagtacgt gtcaaagacc
      241 gaaaagaaat ctgtgatgac tttaaggctg tttatcaagc taactcaaaa gaagaagcga
      301 ataccttctt atccggcatg attgagaaat ggaagaaaaa ctatcctaaa gtgacgcagt
      361 cactcataga aaaccaagac ttattaactt tttatgattt tccacctagc attcgtagaa
      421 ccatttactc aaccaatcta atcgagtctt tcaataagca aattaaaaga tacagccgta
      481 gaaaagagca gtttcaaaat gaagaatcac tagaacgctt tctagtcagc atttttgata
      541 catacaatca aaaatttcta aacagaagcc ataaaggttt tcaacaggta accgatacat
      601 tagtttcaat gtttactgag taactaatta ttttgcagga ggac
//
LOCUS       DACEZN010000339          608 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.361, whole genome shotgun sequence.
ACCESSION   DACEZN010000339 DACEZN010000000
VERSION     DACEZN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.361"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..452)
                     /locus_tag="IXH60_002942"
     CDS             complement(<1..452)
                     /locus_tag="IXH60_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991804.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQ"
BASE COUNT          192 a          113 c          111 g          192 t
ORIGIN      
        1 ttttgagagc ttccaatgtg tttgatcgca tccagtattt ttccagtttc tcgattaact
       61 aagacgatcg tactatgacg tcccattaat tcaacgatca atatgatatt ttgtagatca
      121 cccaactcgt ttcgtttagc aaagtggaaa tggatcactc gatcattttc gatttgttcg
      181 atgtcttcta aaatagcgcc atccaaatat tttcgaagca tcatcacgaa gttaggcgga
      241 ttatcaggat tctgataatc gatttgggta atctgtacac gagcataact tgggtgcgca
      301 gataataata atcgatggtt tttgccgcgt gaacgaatca ccagcacgat ttcattttca
      361 taaggctgat ggattttcga gatccggcca gatagtaacg tttcacgcaa ttcgttgacc
      421 atcgcgtggg taaatacacc atcaaaggac atggttcatt cctcatttct tttcataagt
      481 atattcatta taacgaaaaa aagaagaaaa accaatttgg ccgaatagta aagcaaaagt
      541 ctagaaaaat cttggactaa ttacataaaa aacagtgttt ctatcgttga aatccaaatt
      601 tttaggta
//
LOCUS       DACEZN010000340          592 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.362, whole genome shotgun sequence.
ACCESSION   DACEZN010000340 DACEZN010000000
VERSION     DACEZN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.362"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..579
                     /locus_tag="IXH60_002943"
     CDS             <1..579
                     /locus_tag="IXH60_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1991805.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          203 a          105 c          122 g          162 t
ORIGIN      
        1 ggaatcaaat cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc
       61 ggttatcttt ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt
      121 tttacggatg ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca
      181 gtaattgatc caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac
      241 gcccacgttg atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac
      301 attgctaatt ataaatacga gtatattttc attatggact acaatactgg aaaagaacta
      361 gctagagtaa gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta
      421 ggaaatgttg gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac
      481 gtcatgatgc gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt
      541 catgacaagc gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DACEZN010000341          584 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.363, whole genome shotgun sequence.
ACCESSION   DACEZN010000341 DACEZN010000000
VERSION     DACEZN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.363"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(35..295)
                     /locus_tag="IXH60_002944"
     CDS             complement(35..295)
                     /locus_tag="IXH60_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991806.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          247 a           85 c           66 g          186 t
ORIGIN      
        1 attataagaa aaagaaactt ttggttaatt tatttcatcc cagtgcaaaa tataaaaaag
       61 taaaatagac cataggcaat agattaacaa ttaatataat acaagcaaat attctattca
      121 ttgtatttct gctttcaaaa agttctaata ttgaaagtat aattccacaa atgcctataa
      181 taagtatggg aataagatta gaaaataaca ccccgagcca atctgtgaaa attactatat
      241 ttactaatat ccctattaac atacctatca tatttattaa atcaattttt ttcattagat
      301 atttctcctt tattaaatct gaagtactta tattaaaata attctaaact atttacctca
      361 aaaagtgcaa taaattattt ctgaacaata aaatgatagt caaaacaaaa aaatattgtt
      421 aatcgttcag agccatccga gagtgtaaaa tattttgtgt aaatgaaaaa atccatacaa
      481 aaaaggaagg cgcttctgta gaataaagtt aacgacaacc aattcacaga aaagaggact
      541 tccctatgaa tgattttact acagaaattg tgcaaactct agtc
//
LOCUS       DACEZN010000342          583 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.364, whole genome shotgun sequence.
ACCESSION   DACEZN010000342 DACEZN010000000
VERSION     DACEZN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.364"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            98..>583
                     /locus_tag="IXH60_002945"
     CDS             98..>583
                     /locus_tag="IXH60_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1991807.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YA"
BASE COUNT          261 a           69 c           84 g          169 t
ORIGIN      
        1 tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt caaatataga
       61 agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa aagaaaagga
      121 atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag agaaaaataa
      181 tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac tcaataaact
      241 aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg ataaaattaa
      301 taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt ttaaacttca
      361 agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag aacaacaaaa
      421 agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg atcgtgaaaa
      481 gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat taatcgcata
      541 tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gca
//
LOCUS       DACEZN010000343          583 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.365, whole genome shotgun sequence.
ACCESSION   DACEZN010000343 DACEZN010000000
VERSION     DACEZN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.365"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(308..571)
                     /locus_tag="IXH60_002946"
     CDS             complement(308..571)
                     /locus_tag="IXH60_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ1991808.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          100 c           78 g          181 t
ORIGIN      
        1 tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt ttttcatgtt
       61 gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa cactaaatga
      121 aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg aaataaggga
      181 aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta tttgtttccg
      241 atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag tcgcctttag
      301 tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat gatttggctg
      361 tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca atagaaatga
      421 ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct gcataagaag
      481 aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca aaacctaaaa
      541 ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACEZN010000344          580 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.366, whole genome shotgun sequence.
ACCESSION   DACEZN010000344 DACEZN010000000
VERSION     DACEZN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.366"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..296
                     /locus_tag="IXH60_002947"
     CDS             <1..296
                     /locus_tag="IXH60_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1991809.1"
                     /translation="TRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEGFN
                     KQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          209 a          101 c           69 g          201 t
ORIGIN      
        1 tgactaggaa atggaaaaag cagtatccaa gagtcgtcaa tttactcttg aatcctgccc
       61 tattaacctt ttataatttc cctcacgcca tcagacgaac aatttattcg acgaacctga
      121 ttgaaggctt taataagcag ctaaaacgat atactcgaag aaaagaacaa ttccctaatg
      181 aagaatctct agagagattc ttcatttctc aatttaatca atataaccaa aaatttttag
      241 gtagaattca caagggattt aaagaaattc aggatacatt agagtcgatg atttaactgt
      301 aattaacaga atggattttc catttacata taattcttga cgctaccata atctaaataa
      361 ccatcatatt catctactac ttatataatt gagttttatt aaacttttta agtttctatt
      421 ctatcaaata tttaaacaat ctctgcatcc gaaaagacta cctcgtatag ttttactaac
      481 aatattttta ttagcgtgtt caaatcctga tttcttgtat caattatttt ctgttcactt
      541 tcaataagaa aaaagtcttt acttccatgg ataataataa
//
LOCUS       DACEZN010000345          560 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.367, whole genome shotgun sequence.
ACCESSION   DACEZN010000345 DACEZN010000000
VERSION     DACEZN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.367"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(281..478)
                     /locus_tag="IXH60_002948"
     CDS             complement(281..478)
                     /locus_tag="IXH60_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1991810.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          194 a          102 c           77 g          187 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aattttttct accgacataa
      241 acaaaaaaac cacctcatat gaggcggtta acgttcaaca ttattttacg atatttactg
      301 cttgaggacc acgttgtcct tcttcaatat cataagtaac tgcttggcct tcatctaaag
      361 acttaaagcc ttctccttgg attgctgaga aatgagcgaa tacgtcattt ccatcttcac
      421 cagtgatgaa accaaaacct ttatctgagt taaaccattt tactgtaccg ttattcatat
      481 atatttcctc ctgatacgta tataatacgt gttttattgc aattaatatt acttggtaaa
      541 aggagttttt atagtgtgaa
//
LOCUS       DACEZN010000346          548 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.368, whole genome shotgun sequence.
ACCESSION   DACEZN010000346 DACEZN010000000
VERSION     DACEZN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.368"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>548)
                     /locus_tag="IXH60_002949"
     CDS             complement(<1..>548)
                     /locus_tag="IXH60_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1991811.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DACEZN010000347          536 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.369, whole genome shotgun sequence.
ACCESSION   DACEZN010000347 DACEZN010000000
VERSION     DACEZN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.369"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(18..371)
                     /gene="tnpB"
                     /locus_tag="IXH60_002950"
     CDS             complement(18..371)
                     /gene="tnpB"
                     /locus_tag="IXH60_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1991812.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          155 a          119 c           92 g          170 t
ORIGIN      
        1 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
       61 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      121 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      181 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      241 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      301 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      361 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      421 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      481 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DACEZN010000348          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.370, whole genome shotgun sequence.
ACCESSION   DACEZN010000348 DACEZN010000000
VERSION     DACEZN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.370"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(5..90)
                     /locus_tag="IXH60_002951"
     tRNA            complement(5..90)
                     /locus_tag="IXH60_002951"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IXH60_002952"
     tRNA            complement(107..178)
                     /locus_tag="IXH60_002952"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IXH60_002953"
     tRNA            complement(190..262)
                     /locus_tag="IXH60_002953"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IXH60_002954"
     tRNA            complement(280..361)
                     /locus_tag="IXH60_002954"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IXH60_002955"
     tRNA            complement(369..441)
                     /locus_tag="IXH60_002955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IXH60_002956"
     tRNA            complement(445..517)
                     /locus_tag="IXH60_002956"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACEZN010000349          527 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.371, whole genome shotgun sequence.
ACCESSION   DACEZN010000349 DACEZN010000000
VERSION     DACEZN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.371"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            258..527
                     /locus_tag="IXH60_002957"
                     /pseudo
     CDS             258..527
                     /locus_tag="IXH60_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          181 a           94 c           76 g          176 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataac
//
LOCUS       DACEZN010000350          514 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.372, whole genome shotgun sequence.
ACCESSION   DACEZN010000350 DACEZN010000000
VERSION     DACEZN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.372"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          176 a           67 c          105 g          166 t
ORIGIN      
        1 ggtatgtggt taaaaaagaa aaatagctag gagatttttg agagatgact ggtaaattta
       61 aaaatgatag aaatggagag tatgtatgag taataaacaa tttttatggt cttcttttgc
      121 atttattgtt gttgatattt ttttccgata ttttaataac gatatgatat ttaactggca
      181 aagaattata atcgattact gtattttttg tataatatat tttttagtga aaacatttct
      241 aaaatacgca aaaaaataaa attgaagatg ttattaaatc gcgattattt acggagattt
      301 atataaataa aaaccccctg acgaagtcga agggggtttt tattttggtt tgaggtttgc
      361 atacctaccg aaaaggtagg gcagcaaatc gtcaaacagg tatcagctaa ttgcaccata
      421 agggtgcgct gatactcggc tcaaaagagc cgaaagagcc gtctgcaaga cccctcggcg
      481 agcccacact ttgcttgcaa agtatagtgg gtta
//
LOCUS       DACEZN010000351          511 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.373, whole genome shotgun sequence.
ACCESSION   DACEZN010000351 DACEZN010000000
VERSION     DACEZN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.373"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>511)
                     /locus_tag="IXH60_002958"
     CDS             complement(<1..>511)
                     /locus_tag="IXH60_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAQ1991813.1"
                     /translation="TRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTA
                     CNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEK
                     KDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDN
                     AVVESTYKSV"
BASE COUNT          143 a          119 c           93 g          156 t
ORIGIN      
        1 tgacagattt ataggttgat tccacaacgg cattatcata cggacaaccc ttcctactca
       61 atgaacgagt aattccaaaa ccattcagaa tctcatgaat ggtttggtta tcaaattcct
      121 ttccccggtc tgtatgaaaa agcttgacgt ctgttaaaga atacggtatc cgtccaaagg
      181 cttcttttac caatgaggca tctttcttct caccacagga ataaccaata atttctcgat
      241 taaacaaatc aagtattaag cagatataat gccacttttt ccccacgcga acataagtaa
      301 gatccgtaac gatggcttcc aatggctgtt cttgtgtaaa ggtccgatct aatacattcg
      361 ctgttttcgc ttcattacaa gctgttcgtt gccctttaaa atgagcgatc gtataggtag
      421 atgtcaatcc gcggcgtttc atgattcgac cgattcggcg ccgactgagt tgaagcccac
      481 gctttgctaa acacttcttc aattttcggg t
//
LOCUS       DACEZN010000352          504 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.374, whole genome shotgun sequence.
ACCESSION   DACEZN010000352 DACEZN010000000
VERSION     DACEZN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.374"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          226 a           70 c           81 g          127 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actc
//
LOCUS       DACEZN010000353          489 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.375, whole genome shotgun sequence.
ACCESSION   DACEZN010000353 DACEZN010000000
VERSION     DACEZN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.375"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..415)
                     /locus_tag="IXH60_002959"
     CDS             complement(<1..415)
                     /locus_tag="IXH60_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991814.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          186 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat
       61 ttttatattc aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag
      121 ttgtcaaaga gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat
      181 tatatcgtcc attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg
      241 cgttgacttg ttcttgatta gttaaaaata tcaataaagg aatacttacc caatatattt
      301 ttctatttat ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa
      361 atgctactgg ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg
      421 tcaatggtat taaacagtat atagagcaaa ccaataataa atttttaaca tttattttct
      481 caaaaacat
//
LOCUS       DACEZN010000354          487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.376, whole genome shotgun sequence.
ACCESSION   DACEZN010000354 DACEZN010000000
VERSION     DACEZN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.376"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>487)
                     /locus_tag="IXH60_002960"
     CDS             complement(<1..>487)
                     /locus_tag="IXH60_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ1991815.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACEZN010000355          469 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.378, whole genome shotgun sequence.
ACCESSION   DACEZN010000355 DACEZN010000000
VERSION     DACEZN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.378"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(45..>469)
                     /locus_tag="IXH60_002961"
     CDS             complement(45..>469)
                     /locus_tag="IXH60_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1991816.1"
                     /translation="DEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTA
                     LKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKE
                     NSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          166 a           71 c           24 g          208 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcat
//
LOCUS       DACEZN010000356          467 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.379, whole genome shotgun sequence.
ACCESSION   DACEZN010000356 DACEZN010000000
VERSION     DACEZN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.379"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            1..467
                     /locus_tag="IXH60_002962"
                     /pseudo
     CDS             1..467
                     /locus_tag="IXH60_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          191 a           84 c           87 g          105 t
ORIGIN      
        1 tctaagagaa cacgaagaac tttttcacaa gaattcaagc aacaaatcgt caatctttac
       61 ttagctggaa agccacgtgt agaaatcatt cgagaatatg aactaacggc ttcagcattt
      121 gacaaatggg taaagcaatc taaaacgagt ggttcattca aagaaaaaga taatcttacg
      181 cctgaacaaa aagaattgtt agaactacgt aaaagaaacc agcaattaga aatggaaaat
      241 gatattttaa agcaagcagc gctgatattc ggacgaagag acaagtaatc gatgcgaata
      301 agcatcttta ccctatatca gcgatgtgca gaatattagg tctatcacgt cagtcctatt
      361 attatcaatc aaaaccaaag aaagacgaat cagaacttga agaagtagtc gctgaagaat
      421 ttatccgcag ccgaaaggcc tacggctcaa gaaaaataaa aaaagcc
//
LOCUS       DACEZN010000357          462 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.380, whole genome shotgun sequence.
ACCESSION   DACEZN010000357 DACEZN010000000
VERSION     DACEZN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.380"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..430)
                     /locus_tag="IXH60_002963"
     CDS             complement(<1..430)
                     /locus_tag="IXH60_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1991817.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACEZN010000358          460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.381, whole genome shotgun sequence.
ACCESSION   DACEZN010000358 DACEZN010000000
VERSION     DACEZN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.381"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(20..91)
                     /locus_tag="IXH60_002964"
     tRNA            complement(20..91)
                     /locus_tag="IXH60_002964"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IXH60_002965"
     tRNA            complement(113..200)
                     /locus_tag="IXH60_002965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IXH60_002966"
     tRNA            complement(225..298)
                     /locus_tag="IXH60_002966"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IXH60_002967"
     tRNA            complement(312..382)
                     /locus_tag="IXH60_002967"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IXH60_002968"
     tRNA            complement(384..459)
                     /locus_tag="IXH60_002968"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACEZN010000359          453 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.382, whole genome shotgun sequence.
ACCESSION   DACEZN010000359 DACEZN010000000
VERSION     DACEZN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.382"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            15..317
                     /locus_tag="IXH60_002969"
     CDS             15..317
                     /locus_tag="IXH60_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1991818.1"
                     /translation="MYVAKAERKDRIKEEVDEIICWLTGYSQTELEYQLQKQVTFEQF
                     FAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMIDELAKGKKMEKILRS"
BASE COUNT          161 a           70 c           89 g          133 t
ORIGIN      
        1 caagcgttta tcctatgtat gtagcaaaag cggagagaaa agatcgtata aaagaggaag
       61 tagatgaaat catttgttgg ctgacgggtt attcccaaac agaactagaa tatcaactac
      121 aaaaacaagt gactttcgaa caattttttg cggaagcccc tgtccccaat ccttcacgtt
      181 ccttgattac aggtgttgtt tgtggtgtac gagtagaaga catcgaggat aacttgatga
      241 aagaaattcg ttatttagat aaaatgatcg atgagctagc aaaaggcaaa aaaatggaaa
      301 agatcctcag aagttgattt ttccagagat attcttctcc ttcgctcatt ttgtaacatt
      361 taatttggta aaatgactag ttcacgttaa aataagtcaa aggaggtttt cttatatgtt
      421 tgagacgatt aaacaaaata ttcaaaaata cgc
//
LOCUS       DACEZN010000360          434 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.383, whole genome shotgun sequence.
ACCESSION   DACEZN010000360 DACEZN010000000
VERSION     DACEZN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.383"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            132..>434
                     /locus_tag="IXH60_002970"
     CDS             132..>434
                     /locus_tag="IXH60_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991819.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVT"
BASE COUNT          156 a           47 c           49 g          182 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt ttaaaaaaat aaatgatact atatgtaata taaaaaaaca
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacg
//
LOCUS       DACEZN010000361          430 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.384, whole genome shotgun sequence.
ACCESSION   DACEZN010000361 DACEZN010000000
VERSION     DACEZN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.384"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          150 a           57 c           89 g          134 t
ORIGIN      
        1 tctatgagat aacgaaaatg gcctgtataa taaagaagag gcctagaaga acatcataca
       61 ataagatagc tttcaaaata ctcattgaaa gaaggaaata ctataacgta agaaaggaga
      121 tgaataaaat gaaaaacctg attgctggcg gctttttgtt tgtgggtggt ggcatactta
      181 tgaatacaga taactttggg aatgtcatcc cttttcttgg tgcagcttgt gctttgattg
      241 gtgtaggaat gctgatttat ttcgtattta gcgatgatgg gaagtatggt tgattatgag
      301 tagccaactc tactctttcg taaagattac aacatacgtg acaaaaacag aaagcttgat
      361 tcatcataat aataaaagaa aaagaaaccc aagtgattgg atttcttttt ctttgcataa
      421 gctttattta
//
LOCUS       DACEZN010000362          415 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.385, whole genome shotgun sequence.
ACCESSION   DACEZN010000362 DACEZN010000000
VERSION     DACEZN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.385"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..339)
                     /locus_tag="IXH60_002971"
     CDS             complement(<1..339)
                     /locus_tag="IXH60_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1991820.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACEZN010000363          406 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.386, whole genome shotgun sequence.
ACCESSION   DACEZN010000363 DACEZN010000000
VERSION     DACEZN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.386"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>406
                     /locus_tag="IXH60_002972"
     CDS             <1..>406
                     /locus_tag="IXH60_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1991821.1"
                     /translation="KAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYG
                     ELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHY
                     TPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFM"
BASE COUNT          149 a           73 c           68 g          116 t
ORIGIN      
        1 aaagcgataa acacactcct acggactgaa ttaacggctt ttttagatta cgaaaaatat
       61 gatcgcactg gttttaattc aggtaattcg agaaacggtt cttactttcg atcaatcaaa
      121 accgaatatg gtgaattaac attggaaata cctagagatc gtaatggtga gtttaaacaa
      181 caaactttac cagcctacaa aagaacaaac gatacattgg aaaccactat tatccattta
      241 ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga tcgaaaaaat gtacggtcat
      301 cactatactc cacaaaccat gtccaacatg actaaagttc tgactgaaga agtaaatgcc
      361 tttaaatcca gagccttaaa tgataagtat gtcgctattt ttatgg
//
LOCUS       DACEZN010000364          395 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.387, whole genome shotgun sequence.
ACCESSION   DACEZN010000364 DACEZN010000000
VERSION     DACEZN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.387"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          130 a           76 c           66 g          123 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgt
//
LOCUS       DACEZN010000365          392 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.388, whole genome shotgun sequence.
ACCESSION   DACEZN010000365 DACEZN010000000
VERSION     DACEZN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.388"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            2..370
                     /locus_tag="IXH60_002973"
     CDS             2..370
                     /locus_tag="IXH60_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1991822.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          127 a           59 c           87 g          119 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt tt
//
LOCUS       DACEZN010000366          381 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.389, whole genome shotgun sequence.
ACCESSION   DACEZN010000366 DACEZN010000000
VERSION     DACEZN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.389"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(21..94)
                     /locus_tag="IXH60_002974"
     tRNA            complement(21..94)
                     /locus_tag="IXH60_002974"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IXH60_002975"
     tRNA            complement(108..181)
                     /locus_tag="IXH60_002975"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IXH60_002976"
     tRNA            complement(209..281)
                     /locus_tag="IXH60_002976"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IXH60_002977"
     tRNA            complement(301..374)
                     /locus_tag="IXH60_002977"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DACEZN010000367          373 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.390, whole genome shotgun sequence.
ACCESSION   DACEZN010000367 DACEZN010000000
VERSION     DACEZN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.390"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            141..>373
                     /locus_tag="IXH60_002978"
     CDS             141..>373
                     /locus_tag="IXH60_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991823.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEF"
BASE COUNT          171 a           47 c           53 g          102 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaaat tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 acaaagaatt taa
//
LOCUS       DACEZN010000368          372 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.391, whole genome shotgun sequence.
ACCESSION   DACEZN010000368 DACEZN010000000
VERSION     DACEZN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.391"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            75..>372
                     /locus_tag="IXH60_002979"
     CDS             75..>372
                     /locus_tag="IXH60_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1991824.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRY"
BASE COUNT          132 a           67 c           58 g          115 t
ORIGIN      
        1 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
       61 tttgcaaaac cactttgctc aatgatatca ataacataaa aaaagaattt gaagaacaga
      121 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      181 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      241 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      301 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      361 tcgaccgcta ct
//
LOCUS       DACEZN010000369          362 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.393, whole genome shotgun sequence.
ACCESSION   DACEZN010000369 DACEZN010000000
VERSION     DACEZN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.393"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>362
                     /locus_tag="IXH60_002980"
     CDS             <1..>362
                     /locus_tag="IXH60_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1991825.1"
                     /translation="MQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSY
                     WQSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSW
                     FESIKDILKSIDPGGVMLA"
BASE COUNT          130 a           44 c           82 g          106 t
ORIGIN      
        1 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
       61 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      121 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      181 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      241 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      301 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      361 gc
//
LOCUS       DACEZN010000370          360 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.394, whole genome shotgun sequence.
ACCESSION   DACEZN010000370 DACEZN010000000
VERSION     DACEZN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.394"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..318
                     /locus_tag="IXH60_002981"
     CDS             <1..318
                     /locus_tag="IXH60_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991826.1"
                     /translation="KAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFIG
                     KRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITHL
                     VKK"
BASE COUNT          137 a           35 c           69 g          119 t
ORIGIN      
        1 aaagcaatca ttcctgagaa cttagatgag gttaaacttt atttatggga agataacgga
       61 gaaattgtcg gttttagtgg tgtaagtgca gaagaattag ttatgctatt tcttgatcca
      121 gattttattg gtaagcgata tggtagtcaa atattaattg agctgattga aactgaagat
      181 ataaaaagaa ttgatgttaa tagtcaaaat aaatatgcaa aacattttta tttaagccat
      241 ggatttgaaa ttgaatctga agaagactta gatggttttg gtaaaccata ccctataacg
      301 catttagtaa aaaaataatt catgaatggt ttattacctg attgaacatg aataaaagta
//
LOCUS       DACEZN010000371          337 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.395, whole genome shotgun sequence.
ACCESSION   DACEZN010000371 DACEZN010000000
VERSION     DACEZN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.395"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            122..328
                     /locus_tag="IXH60_002982"
     CDS             122..328
                     /locus_tag="IXH60_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1991827.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          120 a           43 c           82 g           92 t
ORIGIN      
        1 gataaaattg aacaactgga cgggcaaatt gatgccttgc tttcagaaag agaaatattg
       61 atagaagaaa acaagcgatt gaaaaagaaa gtagcccaac caatgaaggg aggtggatag
      121 tatgaacaat agaacctttg atattttgaa atggatcgct atagtagtta tccccgcttt
      181 agcgactttt gtcggtcttg tcggaaaagg tgttaactgg cggtacacag atataacggt
      241 gatgattatt acaggttttg gagcatttct agggagtatt ttaggtgtat caaatcgaac
      301 gtataagaaa atttcatcag ataaatagga ggataga
//
LOCUS       DACEZN010000372          330 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.396, whole genome shotgun sequence.
ACCESSION   DACEZN010000372 DACEZN010000000
VERSION     DACEZN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.396"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          125 a           56 c           43 g          106 t
ORIGIN      
        1 cgctatattt aagatttcaa acaactatat taataggaag aaaatttcat ttttcctctt
       61 agttctataa aaagtgtggc taatttgagg caaaatatta aattaaattt gccacaaatt
      121 aatggctcca ctattatttt aaatcaagaa gtagtaaata atcttatttt ttactaccag
      181 actgtcttta gatattagct tttcgtcacc cactacagtt gacatagacc cacaaaaaac
      241 ttaaaaacct tgcgatgtaa gactggcacg gagaagacca atctatcatc acaaggtaag
      301 agtattcaaa tcttattgaa gactcaaata
//
LOCUS       DACEZN010000373          316 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.397, whole genome shotgun sequence.
ACCESSION   DACEZN010000373 DACEZN010000000
VERSION     DACEZN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.397"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(48..>316)
                     /locus_tag="IXH60_002983"
     CDS             complement(48..>316)
                     /locus_tag="IXH60_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991828.1"
                     /translation="FDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLYYSNKNYFKIPA
                     TKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          101 a           61 c           48 g          106 t
ORIGIN      
        1 tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta atctttatac
       61 ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc gctccgtgcg
      121 tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat tctggttttt
      181 gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa taagctatct
      241 tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta
      301 ttcgaaggat caaatt
//
LOCUS       DACEZN010000374          313 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.398, whole genome shotgun sequence.
ACCESSION   DACEZN010000374 DACEZN010000000
VERSION     DACEZN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.398"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          102 a           51 c           47 g          113 t
ORIGIN      
        1 tcaccgcgaa tttactaaaa ttatgaataa ttatgttaat cttccgaaat ttttttccaa
       61 aattaaggga tcaagctggt gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt
      121 ttaaacaatt atagaaaacc gatactctgt taagttggtt gacacggtgt cgatttttat
      181 gactttctta ttctgaaact tcactaacgg acagtaccta aaaagtctat agtatggacg
      241 tttacgagta ctattttatc aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact
      301 acccccactt agc
//
LOCUS       DACEZN010000375          305 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.399, whole genome shotgun sequence.
ACCESSION   DACEZN010000375 DACEZN010000000
VERSION     DACEZN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.399"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..172
                     /locus_tag="IXH60_002984"
     CDS             <1..172
                     /locus_tag="IXH60_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HAQ1991829.1"
                     /translation="LNEIIYNLKRRNISATNSNTSTISSASEIRKYKELLDDGIITEE
                     EFNKKKQELLDL"
BASE COUNT          129 a           34 c           38 g          104 t
ORIGIN      
        1 tttaaacgaa attatttata atttaaaaag aagaaatatt tctgctacaa attccaatac
       61 atcaacaatt tcctcagctt cagaaataag aaaatataaa gaactattgg atgatggaat
      121 cattacagaa gaagaattta ataaaaagaa acaagaatta ttagatttat aatatctatc
      181 aaagaagatg ggcatttaat ttagctcatc tttttctatt tacaatacaa acatttgttc
      241 gtatactctt tttgaggtga actttatatg ttaatggaag gaaaaacaca attatggttt
      301 aaatt
//
LOCUS       DACEZN010000376          299 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.400, whole genome shotgun sequence.
ACCESSION   DACEZN010000376 DACEZN010000000
VERSION     DACEZN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.400"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>299
                     /locus_tag="IXH60_002985"
     CDS             <1..>299
                     /locus_tag="IXH60_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1991830.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DACEZN010000377          294 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.401, whole genome shotgun sequence.
ACCESSION   DACEZN010000377 DACEZN010000000
VERSION     DACEZN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.401"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>294)
                     /locus_tag="IXH60_002986"
     CDS             complement(<1..>294)
                     /locus_tag="IXH60_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1991831.1"
                     /translation="YISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVE
                     TVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT           87 a           53 c           50 g          104 t
ORIGIN      
        1 atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac
       61 actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag aacgtgagtt
      121 gaaattttga caacctaatt tttctaagga agaaaaaaca gtctcaactg tttttctttg
      181 ttttccaagt aagctgtggt ctagtttttc tgatttatcc atgtttttac gtggcggaac
      241 ggatatagta atgcccttaa gggcaagctt ttcatggatt ttatggctaa tata
//
LOCUS       DACEZN010000378          283 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.402, whole genome shotgun sequence.
ACCESSION   DACEZN010000378 DACEZN010000000
VERSION     DACEZN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.402"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>283
                     /locus_tag="IXH60_002987"
     CDS             <1..>283
                     /locus_tag="IXH60_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1991832.1"
                     /translation="EEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISIL
                     FSAIISLIYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQ"
BASE COUNT          129 a           27 c           35 g           92 t
ORIGIN      
        1 gaagaaagag aaataaagct taatgatggt aaaaaaacaa atctacaatt tgtttctaca
       61 gctgatatgc aaaaagatgc gaaagattta agccttaaat ttttaccata ttctttatta
      121 atatcaattt tattttctgc tattatttct ttaatttatg caaaatcaat aaaaaataat
      181 atacaagaaa taaaaattgt tactgataaa atgatgaaac ttgataaaaa aatgagttta
      241 aaagttagct ctaatgatga agttggagaa ttaaaacaac aaa
//
LOCUS       DACEZN010000379          274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.404, whole genome shotgun sequence.
ACCESSION   DACEZN010000379 DACEZN010000000
VERSION     DACEZN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.404"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>274)
                     /locus_tag="IXH60_002988"
     CDS             complement(<1..>274)
                     /locus_tag="IXH60_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1991833.1"
                     /translation="LPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFK
                     IGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSK"
BASE COUNT           88 a           37 c           45 g          104 t
ORIGIN      
        1 atttcgaagt ttgtaaagta gtttcttcaa tatttttcaa ttcaagtggt aaatctggtc
       61 ctccaataac atttttattt ttgaacgagg aattaaaaaa aagtgtatga aaaaattttt
      121 catctggggc aaacatacct attttaaaat tcaggtagtc ttgttgattt tgttcataca
      181 tttgtattaa ttctgttagg cattctttcg acaaagccca ccattgagaa ccatgataca
      241 aatcaaattt atggtgtcta atttttgggg gaag
//
LOCUS       DACEZN010000380          274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.405, whole genome shotgun sequence.
ACCESSION   DACEZN010000380 DACEZN010000000
VERSION     DACEZN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.405"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            93..>274
                     /locus_tag="IXH60_002989"
     CDS             93..>274
                     /locus_tag="IXH60_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1991834.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           57 c           44 g           89 t
ORIGIN      
        1 gcttctttta cctatgaaat attttcacgt gttaaagtat tcctcgttga acacaacaaa
       61 taaaactttt taacgaataa aggagatttt tcatgacatc acttaaaact ttactttttg
      121 gaacaacttt ggctgccggc gctgcattct tcatgggaac gactgctcat gcggacgaag
      181 cttataccgt tcaatcaggt gatactctat caacgatttc tcaaaaatac gttggtgata
      241 actccttgat caatgccatt gcagaatcaa attc
//
LOCUS       DACEZN010000381          274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.denovo.406, whole genome shotgun sequence.
ACCESSION   DACEZN010000381 DACEZN010000000
VERSION     DACEZN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.denovo.406"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>274
                     /locus_tag="IXH60_002990"
     CDS             <1..>274
                     /locus_tag="IXH60_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991835.1"
                     /translation="FVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFT
                     KILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNK"
BASE COUNT          107 a           33 c           37 g           97 t
ORIGIN      
        1 ttttgtgaat caccggaaaa tcggttcttc ctttaaacgt aaaattaact tgttaactaa
       61 ccataatcaa tttttctcgg atgatttatc cgtttatgat gaaattttta tacaggggga
      121 aaattatttc acaaaaatac ttttctccaa tgtttacaaa aaaaatccag atgtaaaact
      181 aaattatata gaagatggtt tgggtactta tttgaagaag agtatatttt caacaagtaa
      241 aaacgttcaa agagttttta aaatattaaa taaa
//
LOCUS       DACEZN010000382         3277 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.220, whole genome shotgun sequence.
ACCESSION   DACEZN010000382 DACEZN010000000
VERSION     DACEZN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.220"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_002991"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1991836.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>3277
                     /gene="tet"
                     /locus_tag="IXH60_002992"
     CDS             2039..>3277
                     /gene="tet"
                     /locus_tag="IXH60_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HAQ1991837.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLD"
BASE COUNT         1047 a          503 c          673 g         1054 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgat
//
LOCUS       DACEZN010000383         3277 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.221, whole genome shotgun sequence.
ACCESSION   DACEZN010000383 DACEZN010000000
VERSION     DACEZN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.221"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_002993"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1991838.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>3277
                     /gene="tet"
                     /locus_tag="IXH60_002994"
     CDS             2039..>3277
                     /gene="tet"
                     /locus_tag="IXH60_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HAQ1991839.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLD"
BASE COUNT         1047 a          502 c          674 g         1054 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctacattaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgat
//
LOCUS       DACEZN010000384         3277 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.222, whole genome shotgun sequence.
ACCESSION   DACEZN010000384 DACEZN010000000
VERSION     DACEZN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.222"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_002995"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1991840.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>3277
                     /gene="tet"
                     /locus_tag="IXH60_002996"
     CDS             2039..>3277
                     /gene="tet"
                     /locus_tag="IXH60_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HAQ1991841.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLD"
BASE COUNT         1046 a          503 c          674 g         1054 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgat
//
LOCUS       DACEZN010000385         2488 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.245, whole genome shotgun sequence.
ACCESSION   DACEZN010000385 DACEZN010000000
VERSION     DACEZN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.245"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            245..1213
                     /locus_tag="IXH60_002997"
     CDS             245..1213
                     /locus_tag="IXH60_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1991842.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IXH60_002998"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IXH60_002998"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1991843.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..>2488
                     /locus_tag="IXH60_002999"
     CDS             2243..>2488
                     /locus_tag="IXH60_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1991844.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQW"
BASE COUNT          729 a          475 c          612 g          672 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgg
//
LOCUS       DACEZN010000386         2282 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.253, whole genome shotgun sequence.
ACCESSION   DACEZN010000386 DACEZN010000000
VERSION     DACEZN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.253"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_003000"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1991845.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IXH60_003001"
     CDS             2039..2227
                     /locus_tag="IXH60_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991846.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
BASE COUNT          795 a          351 c          458 g          678 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ct
//
LOCUS       DACEZN010000387         2282 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.254, whole genome shotgun sequence.
ACCESSION   DACEZN010000387 DACEZN010000000
VERSION     DACEZN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.254"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_003002"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1991847.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IXH60_003003"
     CDS             2039..2227
                     /locus_tag="IXH60_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991848.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
BASE COUNT          795 a          350 c          459 g          678 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctacattaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ct
//
LOCUS       DACEZN010000388         2282 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.255, whole genome shotgun sequence.
ACCESSION   DACEZN010000388 DACEZN010000000
VERSION     DACEZN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.255"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_003004"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IXH60_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1991849.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IXH60_003005"
     CDS             2039..2227
                     /locus_tag="IXH60_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1991850.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
BASE COUNT          794 a          351 c          459 g          678 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ct
//
LOCUS       DACEZN010000389         2196 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.257, whole genome shotgun sequence.
ACCESSION   DACEZN010000389 DACEZN010000000
VERSION     DACEZN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.257"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1071)
                     /locus_tag="IXH60_003006"
     CDS             complement(<1..1071)
                     /locus_tag="IXH60_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1991851.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQ"
     gene            1624..2172
                     /gene="aac(6')"
                     /locus_tag="IXH60_003007"
     CDS             1624..2172
                     /gene="aac(6')"
                     /locus_tag="IXH60_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1991852.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          698 a          390 c          432 g          676 t
ORIGIN      
        1 ttgtgtagca tgataagttt ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat
       61 ttcataaggc acatgtttaa tatcatttgt tgccatggct ttatctaaga ttgcttctaa
      121 atcggtcgtg acattgagat tcatgtcggt taacgatact ggaagttggt tctttttcat
      181 aaacacatag attttatcaa acaaatcgaa ctgctgatcc atcatcaata aggtcagtac
      241 accataactg acagatgctc cgtgaaggta tccctccatt ttttctagaa cagtcgtacc
      301 gtaatagaat gagtgtgcca tagcagaatt atagttattg ttaactaaaa cagaaaccaa
      361 tccggtagta acaatgatat ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc
      421 ggcactatct ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc acatttttcc
      481 gatttggatt cctaatctat ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga
      541 ttcgacttct ttggataaag catcgccaat acctgcccat aagtatttat caggtgcttc
      601 ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc
      661 gtccattacg ccttcttcgt tatagatgac acataccgca gtgacgggcg aacagttcga
      721 accaatagtg ggaaaggtga agactggttt agctagtttt tcaccaatga cttttacagt
      781 atcgcaagca cggccaccac cgactgcaaa caggacgtct gctttttgga cactttcatg
      841 ctggatcaac tgttcaatat ttccataagt tgaattaccc ccgtaccaga gactggcgac
      901 aacctctatt tcctgtgtca aagctacttt tattttgttt tctgcctttg aaagtgcagt
      961 cttccctcca atcagcacta cttttgtccc atattctttg acgattccat ttgctttttc
     1021 atacgcatct gttccaatgg tataatttgg taagaaaaca ctttgactca tattcatttc
     1081 cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt
     1141 agatggagta gaggtgaacc taagacagga taagtgatga ataaggtggc atgaacacct
     1201 tgaaatcatc aataagtatc ctaaaaacga acaagtacaa aaacattgaa aacgacaata
     1261 atgaattttt gttcctctta gtatttctac cgattccctt tgcttttttg taattatcta
     1321 aaaattctaa aaattaagat tattatatga aaacaggttt tatttgtcaa ttcataattg
     1381 gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca
     1441 attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat
     1501 tttataagta agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg
     1561 agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga
     1621 cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat
     1681 ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa
     1741 atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt
     1801 attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa
     1861 agctcccgac gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta
     1921 gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg
     1981 ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt
     2041 cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca
     2101 aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca
     2161 aattctcaat aataaaaaga ggttgtgaca gaagta
//
LOCUS       DACEZN010000390         2172 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.260, whole genome shotgun sequence.
ACCESSION   DACEZN010000390 DACEZN010000000
VERSION     DACEZN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.260"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            4..741
                     /gene="erm(B)"
                     /locus_tag="IXH60_003008"
     CDS             4..741
                     /gene="erm(B)"
                     /locus_tag="IXH60_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1991853.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            911..1171
                     /locus_tag="IXH60_003009"
                     /pseudo
     CDS             911..1171
                     /locus_tag="IXH60_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1334..1999
                     /locus_tag="IXH60_003010"
                     /pseudo
     CDS             1334..1999
                     /locus_tag="IXH60_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2035..>2172
                     /locus_tag="IXH60_003011"
     CDS             2035..>2172
                     /locus_tag="IXH60_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1413 domain-containing protein"
                     /protein_id="HAQ1991854.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LL"
BASE COUNT          780 a          354 c          391 g          647 t
ORIGIN      
        1 tacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac
       61 caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt
      121 aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta
      181 gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaacattcg tgtcacttta
      241 attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg
      301 agtattcctt accatttaag cacacaaatt attaaaaaag tggtttttga aagccatgcg
      361 tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga
      421 acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg
      481 gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc
      541 acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat
      601 cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa
      661 gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa tagttatcta
      721 ttatttaacg ggaggaaata attctatgag tcgctttttt aaatttggaa agttacacgt
      781 tactaaaggg aatggagata aattattaga tatactactg acagcttcca agaagctaaa
      841 gaggtcccta gcgcctacgg ggaatttgta tcgataaggg gtacaaattc ccactaagcg
      901 ctcgggaccc cttgtaggaa aatgtcctaa gtgtggcaac aatattgtat taaaaaaatc
      961 gttttatggt tgttcaaatt atcctgaatg taagtttact ttagctgaac attttagaaa
     1021 gaaaaaacta accaaaacga atgtaaaaga attactggag ggaaaagaaa ccctggtaaa
     1081 aggaatcaaa aacaaagaga aaaagcccta caatgccgtt gtaaaaattg gggaaaaggg
     1141 atatattgat tttatctctt tttcaaaata aacatggggc tgacccaaaa gtagctccta
     1201 tatgaaaaat agaggtcttt ccatttagat agtagtggaa agacctctat ttatcttttg
     1261 aaagtgaata caaaaataaa gcacactctg ctataatata ggtgattaat ccatttgaga
     1321 gaggtgcttt atcatgtatc aaaattatac cataaaccaa ctttgtttgc ctatagatct
     1381 tgaaattaaa ttagaagaaa atgattttgc tcatgccatt gtgcagtttg ttgactctat
     1441 tcccgatgaa gtctttcttc cttattatca atctatgggt agaccccaat atcatcctcg
     1501 tatgctactt tccattattc tttgtgccta tattcaaggg gtttattcgg gccgccaaat
     1561 acaaaatatg ctgatagata gtattcggat gcgctactta tctcaagagc aattccctaa
     1621 ctttcgtact attaatcgtt ttcgcgttca tccgattatg aacgatattc tagatcattc
     1681 atttgttcaa tttcgtgagc tactggttca aagtggctta atcagtggca gtgctctata
     1741 cattgatggt acaaaaattg aagcggatgc gaataaatac tctttcgttt ggaaaaaaag
     1801 tattttaaag tataaggata gccttgatca aaaagccctt gaaaactacc aacaaatggt
     1861 ggaaacaaaa atcttacctg agttaataga tgatttaaag gatgaacttt ctatagaaga
     1921 gattgaaagg attcgtcaat ctttggaaga aaaggaaaat caattaattg aagcgattga
     1981 ccaaaccgaa acagtagaag ggggctgacc cattattaat aaggagtgga caacatgatt
     2041 gatgtaaatg agctattgga agaagctatt agagaaactg aaaatctaaa tgatggcgaa
     2101 gtttttcttg ttaaagattt attcaaggga tatgtgggga ataggatacc cagaaaggat
     2161 cgacttctgc tt
//
LOCUS       DACEZN010000391         2094 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.264, whole genome shotgun sequence.
ACCESSION   DACEZN010000391 DACEZN010000000
VERSION     DACEZN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.264"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..250
                     /locus_tag="IXH60_003012"
     CDS             <1..250
                     /locus_tag="IXH60_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1991855.1"
                     /translation="REEMNDVVKVDSSLAAMQFMLVARDHGYETNPIGGFEADQLAEA
                     FGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            604..2082
                     /gene="msr"
                     /locus_tag="IXH60_003013"
     CDS             604..2082
                     /gene="msr"
                     /locus_tag="IXH60_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1991856.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT          728 a          341 c          449 g          576 t
ORIGIN      
        1 aagagaagaa atgaacgatg tagtcaaagt cgattctagc ttagcggcta tgcaattcat
       61 gttagttgcg cgtgatcatg gctatgaaac aaatccaatt ggtggattcg aagcagatca
      121 gttggcagag gcatttggtt tggataaaga ccgttatgtt ccagttatta ttttatcaat
      181 aggaaaagcg gtagaagaag gttacgagtc tgttcgttta gcaccagaaa agatcactac
      241 gttcgaataa aaggttttca gttactattc agtagctatt gctttttttg aatcgtgtgt
      301 ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa
      361 cttttgaata acaactaaat aaattcaaaa gacatgtata aacaacaatt tcatggatgg
      421 agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa gctatgacag atttggtgat
      481 ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt ttgcaaacag
      541 atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa
      601 catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta
      661 gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa
      721 aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat
      781 gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa
      841 gcaaaggacc taaatttgga attagccagt catttccagt taagactgga agaaacttca
      901 gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct
      961 tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat
     1021 cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc
     1081 tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag
     1141 ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta
     1201 cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag
     1261 aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga
     1321 ccggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat
     1381 gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg
     1441 aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg
     1501 ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag
     1561 ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta
     1621 ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa
     1681 gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg
     1741 agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct
     1801 caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg
     1861 attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt
     1921 attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt
     1981 ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata
     2041 aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgat
//
LOCUS       DACEZN010000392         1851 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.274, whole genome shotgun sequence.
ACCESSION   DACEZN010000392 DACEZN010000000
VERSION     DACEZN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.274"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..331
                     /locus_tag="IXH60_003014"
     CDS             <1..331
                     /locus_tag="IXH60_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1991857.1"
                     /translation="AGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNE
                     VILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVR
                     YMFNKIT"
     gene            429..1802
                     /gene="tet(L)"
                     /locus_tag="IXH60_003015"
     CDS             429..1802
                     /gene="tet(L)"
                     /locus_tag="IXH60_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1991858.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          542 a          274 c          368 g          667 t
ORIGIN      
        1 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
       61 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
      121 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
      181 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
      241 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
      301 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
      361 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
      421 aattaattat gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc
      481 tttgcgtttt atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg
      541 atattgcaaa tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta
      601 tgttaacctt ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca
      661 aaaggttact cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg
      721 gccattcttt cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg
      781 catttccagc actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta
      841 aagcatttgg tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg
      901 gtggaatgat agcccattat attcattggt cctatcttct actcattcct atgataacaa
      961 ttatcactgt tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt
     1021 ttgatatcaa aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa
     1081 catcatatag catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac
     1141 atatcaggaa agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta
     1201 tgattggagt tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg
     1261 ttccttatat gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta
     1321 ttttccctgg aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata
     1381 gaagaggtcc tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa
     1441 ctgcttcctt tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt
     1501 taggtgggct ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac
     1561 agcaggaagc tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa
     1621 caggtattgc aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac
     1681 ctatggaagt tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa
     1741 tcattgtcat tagttggctg gttaccttga atgtatataa acattctcaa agggatttct
     1801 aaatcgttaa gggatcaact ttgggagaga gttcaaaatt gatccttttt t
//
LOCUS       DACEZN010000393         1806 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.280, whole genome shotgun sequence.
ACCESSION   DACEZN010000393 DACEZN010000000
VERSION     DACEZN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.280"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..452
                     /locus_tag="IXH60_003016"
     CDS             <1..452
                     /locus_tag="IXH60_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1991859.1"
                     /translation="AIEDVDFPEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNL
                     VFTVEHPVFTAHGTQDWYYNEKGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTY
                     LNTLLSNSFIINQIVEPQPPENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            485..1397
                     /gene="ant(6)"
                     /locus_tag="IXH60_003017"
                     /pseudo
     CDS             485..1397
                     /gene="ant(6)"
                     /locus_tag="IXH60_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            1394..>1806
                     /locus_tag="IXH60_003018"
     CDS             1394..>1806
                     /locus_tag="IXH60_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991860.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLET"
BASE COUNT          642 a          262 c          408 g          494 t
ORIGIN      
        1 gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg
       61 cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg
      121 ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag
      181 actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg
      241 agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga
      301 ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac
      361 agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga
      421 tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat
      481 gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga
      541 acgaacgtat tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag
      601 atgaatttca ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta
      661 atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg
      721 aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata
      781 aaattgatct taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat
      841 taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata
      901 tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt
      961 ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg
     1021 atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca
     1081 tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat
     1141 ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat
     1201 gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa
     1261 ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt
     1321 ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag
     1381 agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc
     1441 cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac
     1501 ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga
     1561 agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga taatatatct
     1621 ttactaccaa gacgataaat gcgtcggaaa agttaaactg cgaaaaaatt ggaaccggta
     1681 cgcttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc
     1741 gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga
     1801 aaccca
//
LOCUS       DACEZN010000394         1661 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.292, whole genome shotgun sequence.
ACCESSION   DACEZN010000394 DACEZN010000000
VERSION     DACEZN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.292"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            157..1659
                     /locus_tag="IXH60_003019"
     CDS             157..1659
                     /locus_tag="IXH60_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1991861.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
BASE COUNT          587 a          293 c          360 g          421 t
ORIGIN      
        1 agactgtacc ttgtggagac aaggaagcag tgtgcagatt ttcgggattg gcttggtttt
       61 ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca tcctctttag ccagttctga
      121 aaataggact ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga aataaaaaat
      181 ctgacattcg gctacgacag ccaaggcaca ttattatttg aacaagcaaa tctaaatttt
      241 gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac aactttactg
      301 aatattctac aaaacaaact accttatcaa gggcaagtaa tccatcagca agaatttgcc
      361 tatttcccgc aacagacaaa agataaagaa cgtttaacct attacgtgtt aaatgatatt
      421 acggattttg agatatggga aatcgaaaga gagctccaat tgatgcaaac agatcctgaa
      481 atcttatgga gagaattcag cacactatcg gggggagaga agacaaaagt cctactggca
      541 cttttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa tcatttggat
      601 atctctggta gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg
      661 gtcagccatg accggggatt tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa
      721 agtcaactgg aactttatca agggaatttc tctatctatg aagaacagaa aaaacttcgt
      781 gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa
      841 acagcagctg aaaaagccga atggtctcgt tcccgagaag gagataaaac aaagaaacaa
      901 gtcggattca tcgatactga atctagacga gtgaataaag gagcagtggg tgctgatgct
      961 gcacggacga tgaaacgatc caaagcaatc gtgaatcgga tggagaccca gatcagcgag
     1021 aaagaaaaac tattaaaaga tatcgaatat atcgattctt tgacgatgaa tagccaagcg
     1081 tctcaccata agcgactttt aagcgtagaa gatcttcaat tagggtatga aaatctgtta
     1141 ttcgagccaa ttcattttac aatcgagcct catcagcggg tggcgatttc aggtcctaac
     1201 ggtgcaggaa agtcatccat tatccattat cttctggggg cattcaacgg caaggttata
     1261 ggagaaaaat accagccaaa acatctgagc attagttatg caagccaaaa ttatgaagac
     1321 aatcgaggaa cgttggcgga atttgcagag aaaaaccaag tagactacca agcatttttg
     1381 aacaacctcc gaaagcttgg gatggaaaga gatgtttttc ataacaagat cgagcagatg
     1441 agtatgggcc aacggaaaaa agtggaattg gctaaatctt tatcacagcc agctgaacta
     1501 tatacatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca attagaacaa
     1561 ctgatcttga acgtgaaacc tgccatgtta ctagtggaac atgatcaaac cttcctggat
     1621 aaagtatcta ctgagattat ttctcttgag agaatctaac c
//
LOCUS       DACEZN010000395         1530 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.300, whole genome shotgun sequence.
ACCESSION   DACEZN010000395 DACEZN010000000
VERSION     DACEZN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.300"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            68..610
                     /gene="sat4"
                     /locus_tag="IXH60_003020"
     CDS             68..610
                     /gene="sat4"
                     /locus_tag="IXH60_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1991862.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            703..1497
                     /locus_tag="IXH60_003021"
                     /note="aph(3')-IIIa"
     CDS             703..1497
                     /locus_tag="IXH60_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1991863.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          510 a          256 c          378 g          386 t
ORIGIN      
        1 aatacactgg taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc
       61 gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga
      121 accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac
      181 agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga
      241 tgaggaggca gattgccttg aatatattga caatactgat aagataatat atctttacta
      301 ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta
      361 tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat
      421 caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca
      481 ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt
      541 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
      601 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
      661 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
      721 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
      781 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
      841 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
      901 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
      961 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     1021 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     1081 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     1141 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
     1201 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     1261 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     1321 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     1381 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     1441 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
     1501 ctagatttag atgtctaaaa agctttaact
//
LOCUS       DACEZN010000396         1530 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.301, whole genome shotgun sequence.
ACCESSION   DACEZN010000396 DACEZN010000000
VERSION     DACEZN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.301"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            68..610
                     /gene="sat4"
                     /locus_tag="IXH60_003022"
     CDS             68..610
                     /gene="sat4"
                     /locus_tag="IXH60_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1991864.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETHDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            703..1497
                     /locus_tag="IXH60_003023"
                     /note="aph(3')-IIIa"
     CDS             703..1497
                     /locus_tag="IXH60_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1991865.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          510 a          256 c          377 g          387 t
ORIGIN      
        1 aatacactgg taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc
       61 gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga
      121 accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac
      181 agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga
      241 tgaggaggca gattgccttg aatatattga caatactgat aagataatat atctttacta
      301 ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta
      361 tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat
      421 caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca
      481 tgacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt
      541 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
      601 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
      661 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
      721 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
      781 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
      841 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
      901 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
      961 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     1021 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     1081 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     1141 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
     1201 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     1261 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     1321 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     1381 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     1441 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
     1501 ctagatttag atgtctaaaa agctttaact
//
LOCUS       DACEZN010000397         1386 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.306, whole genome shotgun sequence.
ACCESSION   DACEZN010000397 DACEZN010000000
VERSION     DACEZN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.306"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..242
                     /locus_tag="IXH60_003024"
     CDS             <1..242
                     /locus_tag="IXH60_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAQ1991866.1"
                     /translation="KQLSLTPTEFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNN
                     TITVHIRHLREKMNDTIDNPKYIKTVWGVGYKIEK"
     gene            220..1374
                     /locus_tag="IXH60_003025"
     CDS             220..1374
                     /locus_tag="IXH60_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1991867.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          450 a          269 c          308 g          359 t
ORIGIN      
        1 agaagcagtt atcccttact cccaccgagt tttcaatact gcgaatcctc tgtgaaaaca
       61 aggggaatgt ggttagctcc gagctgctat ttcatgagat atggggcgac gaatatttca
      121 gcaagagcaa caacaccatc accgtgcata tccggcattt gcgcgaaaaa atgaacgaca
      181 ccattgataa tccgaaatat ataaaaacgg tatggggggt tggttataaa attgaaaaat
      241 aaaaaaaacg actattccaa actagaacga aaactttaca tgtatatcgt tgcaattgtt
      301 gtggtagcaa ttgtattcgt gttgtatatt cgttcaatga tccgagggaa acttggggat
      361 tggatcttaa gtattttgga aaacaaatat gacttaaatc acctggacgc gatgaaatta
      421 tatcaatatt ccatacggaa caatatagat atctttattt atgtggcgat tgtcattagt
      481 attcttattc tatgtcgcgt catgctttca aaattcgcaa aatactttga cgagataaat
      541 accggcattg atgtacttat tcagaacgaa gataaacaaa ttgagctttc tgcggaaatg
      601 gatgttatgg aacaaaagct caacacatta aaacggactc tggaaaagcg agagcaggat
      661 gcaaagctgg ccgaacaaag aaaaaatgac gttgttatgt acttggcgca cgatattaaa
      721 acgcccctta catccattat cggttatttg agcctgcttg acgaggctcc agacatgccg
      781 gtagatcaaa aggcaaagta tgtgcatatc acgttggaca aagcgtatcg actcgaacag
      841 ctaatcgacg agttttttga gattacacgg tataacctac aaacgataac gctaacaaaa
      901 acgcacatag acctatacta tatgctggtg cagatgaccg atgaatttta tcctcagctt
      961 tccgcacatg gaaaacaggc ggttattcac gcccccgagg atctgaccgt gtccggcgac
     1021 cctgataaac tcgcgagagt ctttaacaac attttgaaaa acgccgctgc atacagtgag
     1081 gataacagca tcattgacat taccgcgggc ctctccgggg atgtggtgtc aatcgaattc
     1141 aagaacactg gaagcatccc aaaagataag ctagctgcca tatttgaaaa gttctatagg
     1201 ctggacaatg ctcgttcttc cgatacgggt ggcgcgggac ttggattggc gattgcaaaa
     1261 gaaattattg ttcagcatgg agggcagatt tacgcggaaa gcaatgataa ctatacgacg
     1321 tttagggtag agcttccagc gatgccagac ttggttgata aaaggaggtc ctaagagatg
     1381 tatata
//
LOCUS       DACEZN010000398         1231 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.315, whole genome shotgun sequence.
ACCESSION   DACEZN010000398 DACEZN010000000
VERSION     DACEZN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.315"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..468
                     /locus_tag="IXH60_003026"
     CDS             <1..468
                     /locus_tag="IXH60_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAQ1991868.1"
                     /translation="ELDYAIESARQYDSKILIEQAVSGCEVGCAVLGNSAALAVGEVD
                     QIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETAKKIYKALGCRGLAR
                     VDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELIDRLIVLALKG"
     gene            474..1082
                     /locus_tag="IXH60_003027"
     CDS             474..1082
                     /locus_tag="IXH60_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1991869.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          363 a          239 c          306 g          323 t
ORIGIN      
        1 gaattggact acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag
       61 gctgtttcgg gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagctgtt
      121 ggcgaggtgg accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag
      181 ccggaaaaag gctctgaaaa cgcagttata accgttcccg cagacctttc agcagaggag
      241 cgaggacgga tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta
      301 gcccgtgtgg atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact
      361 ctgcccggtt tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca
      421 cttcccgaac tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa
      481 taggatttac ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca
      541 cttgggataa tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga
      601 catacgagtt ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg
      661 gattgcttct atgggacggt taccgtccta atcgtgctgt aaactgtttt atgcaatggg
      721 ctgcacagcc ggaaaataac ctgacaaagg aaagttatta tcccaatatt gaccgaactg
      781 agatgatttc aaaaggatac gtggcttcaa aatcaagcca tagccgcggc agtgccattg
      841 atcttacgct ttatcgatta gacacgggtg agcttgtacc aatggggagc cgatttgatt
      901 ttatggatga acgctctcat catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc
      961 gcagacgttt gcgctccatc atggaaaaca gtgggtttga agcatatagc ctcgaatggt
     1021 ggcactatgt attaagagac gaaccatacc ccaatagcta ttttgatttc cccgttaaat
     1081 aaacttttaa ccgttgcacg gacaaactat ataagctaac tctttcggca ggaaacccga
     1141 cgtatgtaac tggttcttag ggaatttata tatagtagat agtattgaag aggttctgtt
     1201 gcaaagtttt aaataaagaa taaaatccct t
//
LOCUS       DACEZN010000399          898 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.333, whole genome shotgun sequence.
ACCESSION   DACEZN010000399 DACEZN010000000
VERSION     DACEZN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.333"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..164)
                     /locus_tag="IXH60_003028"
     CDS             complement(<1..164)
                     /locus_tag="IXH60_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1991870.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IXH60_003029"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IXH60_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1991871.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          337 a          100 c          166 g          295 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgc
//
LOCUS       DACEZN010000400          880 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.337, whole genome shotgun sequence.
ACCESSION   DACEZN010000400 DACEZN010000000
VERSION     DACEZN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.337"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            12..707
                     /gene="vanR"
                     /locus_tag="IXH60_003030"
     CDS             12..707
                     /gene="vanR"
                     /locus_tag="IXH60_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1991872.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            685..>880
                     /locus_tag="IXH60_003031"
     CDS             685..>880
                     /locus_tag="IXH60_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1991873.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAM"
BASE COUNT          296 a          162 c          192 g          230 t
ORIGIN      
        1 gggtgataac tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt
       61 tggttgaatt atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag
      121 aagcattgga atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc
      181 ccggcacaag cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta
      241 tcatgctgac cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg
      301 atgattatat aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt
      361 tgcgccgata caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact
      421 ccggccttgt cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc
      481 ttactcccac cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta
      541 gctccgagct gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca
      601 ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga
      661 aatatataaa aacggtatgg ggggttggtt ataaaattga aaaataaaaa aaacgactat
      721 tccaaactag aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt agcaattgta
      781 ttcgtgttgt atattcgttc aatgatccga gggaaacttg gggattggat cttaagtatt
      841 ttggaaaaca aatatgactt aaatcacctg gacgcgatga
//
LOCUS       DACEZN010000401          865 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.338, whole genome shotgun sequence.
ACCESSION   DACEZN010000401 DACEZN010000000
VERSION     DACEZN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.338"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            69..542
                     /gene="dfrF"
                     /locus_tag="IXH60_003032"
     CDS             69..542
                     /gene="dfrF"
                     /locus_tag="IXH60_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1991874.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            669..>865
                     /locus_tag="IXH60_003033"
     CDS             669..>865
                     /locus_tag="IXH60_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1991875.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHS"
BASE COUNT          324 a           88 c          187 g          266 t
ORIGIN      
        1 agtttgttaa gatgtggtgc acttgatgga atcacaggta cgacatcata ttatggagga
       61 tgaaatagat gataggtttg attgttgcga ggtcaaagaa taatgttata ggcaagaatg
      121 gtaatatacc atggaaaata aagggagaac aaaagcaatt tagagagtta acaacgggta
      181 atgtggttat tatggggcga aagtcttatg aagaaatcgg tcatccgttg cctaatagaa
      241 tgaatattgt tgtttccacc acaacagagt atcaaggaga taatttagtt tcagttaaat
      301 cattagaaga tgcattatta ttggctaaag gacgagatgt atacatatct ggtggatatg
      361 gactatttaa ggaagctttg caaatagtag ataaaatgta tatcacagaa gtagatttaa
      421 atattgaaga tggagataca ttctttccag aatttgatat caatgatttt gaagttttga
      481 taggggaaac acttggtgag gaagtgaaat atacgagaac attttatgta aggaaaaaat
      541 gaattgagta aattttggat ttagggtgtt ttcataaata tattgctttt tatgcatata
      601 taaattgttg taagacaaat tccagtttgt atggggcgat aaaatcagaa tttgagagag
      661 gattgaatat gaataaggaa tggtctgaac ttaataaaac aatgcaggca caaataaaaa
      721 agaaagatac ttataagagg ggtattgata ctttacttac tttacgaagt cagttaatac
      781 aaaccttagt atctttcaaa gaagaattat gcagagaaga ttttaactca atccccttca
      841 taaacgctga tggttatcat agtaa
//
LOCUS       DACEZN010000402          834 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.342, whole genome shotgun sequence.
ACCESSION   DACEZN010000402 DACEZN010000000
VERSION     DACEZN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.342"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>834
                     /locus_tag="IXH60_003034"
     CDS             <1..>834
                     /locus_tag="IXH60_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1991876.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     Q"
BASE COUNT          349 a           77 c          134 g          274 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaat
//
LOCUS       DACEZN010000403          730 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.351, whole genome shotgun sequence.
ACCESSION   DACEZN010000403 DACEZN010000000
VERSION     DACEZN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.351"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            7..534
                     /locus_tag="IXH60_003035"
     CDS             7..534
                     /locus_tag="IXH60_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1991877.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(597..>730)
                     /locus_tag="IXH60_003036"
     CDS             complement(597..>730)
                     /locus_tag="IXH60_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1991878.1"
                     /translation="GGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT          246 a          130 c          161 g          193 t
ORIGIN      
        1 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
       61 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
      121 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
      181 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
      241 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
      301 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
      361 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
      421 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
      481 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
      541 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
      601 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
      661 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
      721 agcccgcctg
//
LOCUS       DACEZN010000404          482 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST412 isolate 1096
            SAMN10039047-rid9589173.guided.377, whole genome shotgun sequence.
ACCESSION   DACEZN010000404 DACEZN010000000
VERSION     DACEZN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039047
            Sequence Read Archive: SRR7839683
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705116.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 08:26:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,980
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 18 of 75
            Pseudo Genes (multiple problems)  :: 9 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039047-rid9589173.guided.377"
                     /strain="ST412"
                     /isolate="1096"
                     /isolation_source="Facility 1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..477
                     /locus_tag="IXH60_003037"
     CDS             <1..477
                     /locus_tag="IXH60_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HAQ1991879.1"
                     /translation="IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          207 a           26 c           87 g          162 t
ORIGIN      
        1 attgataata aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat
       61 gatttaactg atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca
      121 acagtttttg agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg
      181 ttagatggca ataatagatt aactggaata attgattttg gagattctgg aattatagat
      241 gaatattgtg attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt
      301 ggagaagata tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa
      361 gatatagttg aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa
      421 caggaattta tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta
      481 ta
//
